natgillin/translations-raw huggingface dataset:
Tubercle height is often more jagged but can be somewhat rounded at times.
Koncentracija raztopljenega kisika se v negativnih kontrolah in najvišji preskusni koncentraciji z zarodki, sposobnimi preživetja, izmeri na koncu preskusa, pri čemer mora biti skladna z merili za veljavnost preskusa (glej odstavek 8f).
Rating 3 — pronounced, is usually quite large and rounded with less definition in structure.
Če obstaja dvom, da se temperatura med ploščami s 24 jamicami razlikuje, se temperatura izmeri v treh naključno izbranih posodah.
At times these tubercles will run together forming a single mass along an individual or combination of areas (B, C and D, described below).
Najbolje je, če se temperatura med preskusom beleži stalno oziroma vsaj dnevno.
Coloration and design are similar to rating 2 but at times are fairly indiscriminate.
Pri statičnemu sistemu je treba koncentracijo preskusne kemikalije izmeriti vsaj v najvišji in najnižji preskusni koncentraciji na začetku in na koncu preskusa, vendar je najbolje, če se izmeri pri vseh tretiranjih.
Using this rating system generally will result in overall tubercle scores of < 50 in a normal control male possessing a tubercle count of 18 to 20 (Jensen et al.
Pri polstatičnih preskusih (zamenjavah), pri katerih bi morala koncentracija preskusne kemikalije ostati v območju ± 20 % nominalnih vrednosti, se priporoča, da se analizirata vsaj najvišja in najnižja preskusna koncentracija, ko sta sveže pripravljeni in takoj pred zamenjavo.
2001).
Pri preskusih, pri katerih se pričakuje, da koncentracija preskusne kemikalije ne bo ostala v območju ± 20 % nominalne vrednosti, je treba analizirati vse preskusne koncentracije, ko so sveže pripravljene in takoj pred zamenjavo vode.
The actual number of tubercles in some fish may be greater than the template boxes for a particular rating area.
V primeru nezadostne količine za analizo je lahko koristna združitev testnih raztopin ali uporaba nadomestnih komor iz enakega materiala in z enakim razmerjem med prostornino in površino kot pri ploščah s 24 jamicami.
If this happens, additional rating numbers may be marked within, to the right or to the left of the box.
Zelo se priporoča, naj rezultati temeljijo na izmerjenih koncentracijah.
The template therefore does not need to display symmetry.
Če koncentracije ne ostanejo v območju 80–120 % nominalne koncentracije, je treba učinek koncentracij izraziti glede na geometrijsko sredino izmerjenih koncentracij (glej poglavje 5 v Smernicah OECD za preskušanje toksičnosti zahtevnih snovi in zmesi v vodnem okolju (28)).
An additional technique for mapping tubercles which are paired or joined vertically along the horizontal plane of the mouth could be done by double-marking two tubercle rating points in a single box.
MEJNI PRESKUS
Mapping regions:
S postopki, opisanimi v tej preskusni metodi, se lahko izvede mejni preskus s 100 mg/l preskusne kemikalije ali pri meji topnosti v preskusnem gojišču (pri čemer se uporabi nižja od obeh vrednosti), da se dokaže, da je vrednost LC50 višja od te koncentracije.
A — Tubercles located around eye.
Mejni preskus je treba izvesti pri 20 zarodkih v tretiranju, pozitivni kontroli in po potrebi kontroli s topilom ter 24 zarodkih v negativni kontroli.
Mapped dorsal to ventral around anterior rim of eye.
Če je odstotek smrtnosti v preskušani koncentraciji za 10 % večji od odstotka umrljivosti v negativni kontroli (ali kontroli s topilom), je treba izvesti celotno študijo.
Commonly multiple in mature control males, not present in control females, generally paired (one near each eye) or single in females exposed to androgens.
Vse opažene učinke je treba zabeležiti.
B — Tubercles located between nares, (sensory canal pores).
Če je umrljivost za 10 % večja v negativni kontroli (ali kontroli s topilom), je preskus neveljaven in ga je treba ponoviti.
Normally in pairs for control males at more elevated levels (2- enlarged or 3- pronounced) of development.
Pri tem preskusu se za statistično analizo posamezne jamice štejejo za neodvisne ponovljene vzorce.
Not present in control females with some occurrence and development in females exposed to androgens.
Odstotki zarodkov, pri katerih je pozitivno vsaj eno opažanje na višjem nivoju biološke organizacije pri 48 urah in/ali 96 urah, se lahko prikažejo v odvisnosti od preskusnih koncentracij.
C — Tubercles located immediately anterior to nares, parallel to mouth.
Za izračun naklonov krivulje, vrednosti LC50 in mej zaupanja (95 %) je treba uporabiti ustrezne statistične metode (38) in upoštevati dokument OECD z naslovom „Sodobni pristopi pri statistični analizi podatkov o ekotoksičnosti: smernice za uporabo“ (39).
Generally enlarged or pronounced in mature control males.
kemijski identifikacijski podatki, kot so ime po nomenklaturi IUPAC ali ime CAS, številka CAS, koda po sistemu SMILES ali identifikatorju InChI, strukturna formula, čistost, kemijska identiteta nečistoč, kot je ustrezno in praktično izvedljivo itd. (vključno z vsebnostjo organskega ogljika, če je to ustrezno).
Present or enlarged in less developed males or androgen-treated females.
Preskusni organizmi:
D — Tubercles located parallel along mouth line.
uporabljeni preskusni postopek (npr. polstatična zamenjava);
Generally rated developed in control males.
obdobje osvetljenosti;
Absent in control females but present in androgen-exposed females.
načrt preskusa (npr. število preskusnih komor, vrste kontrol);
E — Tubercles located on lower jaw, close to mouth, usually small and commonly in pairs.
lastnosti kakovosti vode pri vzdrževanju rib (npr. vrednost pH, trdota, temperatura, prevodnost, raztopljeni kisik);
Varying in control or treated males, and treated females.
koncentracija raztopljenega kisika, vrednost pH, skupna trdota, temperatura in prevodnost testnih raztopin na začetku in po 96 urah;
F — Tubercles located ventral to E. Commonly small and paired.
metoda priprave osnovnih raztopin in testnih raztopin ter pogostost zamenjave;
Present in control males and androgen-exposed females.
utemeljitev uporabe in izbire topila, če to ni voda;
Tubercle Template:
nominalne preskusne koncentracije in rezultat vseh analiz za določanje koncentracije preskusne kemikalije v preskusnih posodah; poročati je treba o izkoristku učinkovitosti metode in meji določljivosti (LOQ);
Numerical Rating
dokazi, da so kontrole izpolnjevale merila za veljavnost v zvezi s skupnim preživetjem;
Date
stopnja oplojenosti iker;
resent
odstotek izvalitve v tretiranih in kontrolnih skupinah.
Total Score
najvišja koncentracija, ki med preskusom ne povzroči umrljivosti;
nlarged
najnižja koncentracija, ki med preskusom povzroči 100-odstotno umrljivost;
ronounced
skupna umrljivost za vsako koncentracijo ob priporočenih časih opazovanja;
Appendix 5B
vrednosti LC50 po 96 urah (in neobvezno po 48 urah) za umrljivost s 95-odstotnimi mejami zaupanja, če je to mogoče;
ASSESSMENT OF SECONDARY SEX CHARACTERISTICS IN MEDAKA FOR THE DETECTION OF CERTAIN ENDOCRINE ACTIVE CHEMICALS
graf s krivuljo koncentracija–umrljivost na koncu preskusa;
Below is a description of the measurement of papillary processes [1], which are the secondary sex characteristics in medaka (Oryzias latipes).
umrljivost v kontrolah (v negativnih kontrolah, notranjih kontrolah plošč, pozitivni kontroli in morebitni uporabljeni kontroli s topilom);
After the excision of the liver (Appendix 6), the carcass is placed into a conical tube containing about 10 ml of 10 % neutral buffered formalin (upside: head, downside: tail).
podatki o rezultatih vseh štirih opažanj na višjem nivoju biološke organizacije;
If the gonad is fixed in a solution other than 10 % neutral buffered formalin, make a transverse cut across the carcass between anterior region of anal fin and anus using razor, taking care not to harm the gonopore and gonad itself (Fig.3).
pogostost in opis morfoloških in fizioloških anomalij, če obstajajo (glej primere na sliki 2 v Dodatku 5);
Place the cranial side of the fish body into the fixative solution to preserve the gonad, and the tail side of the fish body into the 10 % neutral buffered formalin as described above.
incidenti med preskusom, ki lahko vplivajo na rezultate;
After placing the fish body into 10 % neutral buffered formalin, grasp the anterior region of the anal fin with tweezers and fold it for about 30 seconds to keep the anal fin open.
statistična analiza in obdelava podatkov (probit analiza, model logistične regresije in geometrijska sredina za LC50);
When grasping the anal fin with tweezers, grasp a few fin rays in the anterior region with care not to scratch the papillary processes.
naklon in meje zaupanja regresije (transformirane) krivulje koncentracija–odziv.
After keeping the anal fin open for about 30 seconds, store the fish body in 10 % neutral buffered formalin at room temperature until the measurement of the papillary processes (measurement should be conducted after fixing for at least 24 hours).
Vsa odstopanja od preskusne metode in ustrezna pojasnila.
Measurement
Razprava in razlaga rezultatov.
After fixing the fish body in the 10 % neutral buffered formalin for at least 24 hours, pick up the fish carcass from the conical tube and wipe the formalin on the filter paper (or paper towel).
OECD (2011).
Place the fish abdomen side up.
Validation Report (Phase 1) for the Zebrafish Embryo Toxicity Test: Part I and Part II.
Then cut the anal fin using small dissection scissors carefully (it is preferable to cut the anal fin with small amount of pterygiophore).
Series on Testing and Assessment No.
Grasp the anterior region of the severed anal fin with tweezers and put it on a glass slide with a several drops of water.
157, OECD, Pariz.
Then cover the anal fin with a cover glass.
OECD (2012).
Be careful not to scratch the papillary processes when grasping the anal fin with tweezers.
Validation Report (Phase 2) for the Zebrafish Embryo Toxicity Test: Part I and Part II (Annexes).
Count the number of the joint plate with papillary processes using the counter under a biological microscope (upright microscope or inverted microscope).
Series on Testing and Assessment No.
The papillary processes are recognised when a small formation of processes is visible on the posterior margin of joint plate.
179, OECD, Pariz.
Write the number of joint plate with papillary processes in each fin ray to the worksheet (e.g. first fin ray: 0, second fin ray: 10, third fin ray: 12, etc.) and enter the sum of this number on the Excel spreadsheet by individual fish.
Environmental Toxicology and Chemistry 32: 1768–1783.
If necessary, take a photograph of the anal fin and count the number of joint plate with papillary processes on the photograph.
Pharmacol.: 149 (2), 196–209
After the measurement, put the anal fin into the conical tube described in (1) and store it.
Poglavje C.4 te priloge: Dobra biološka razgradljivost.
Fig.1.
Poglavje C.29 te priloge: Dobra biološka razgradljivost – CO2 v zaprtih posodah.
Diagram showing sexual difference in shape and size of the anal fin.
Poglavje C.48 te priloge: Kratkotrajni preskus razmnoževanja rib.
A, male; B, female.
Glej Dodatek 4a.
Fig.2.
Dostopen na: [http://www.iso.org].
A, Processes on joint plates of anal fin-ray.
23, OECD, Pariz.
J.P., joint plate; A.S., axial space; P., process.
Evropska komisija (2007).
B, Distal extremity of fin-ray.
Priporočilo Komisije 2007/526/ES z dne 18. junija 2007 o smernicah za bivališča in oskrbo živali, ki se uporabljajo za poskusne in druge znanstvene namene (notificirano pod dokumentarno številko C(2007) 2525) [http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2007:197:0001:0089:SL:PDF]
Actinotrichia (Act.) are on the tip.
Evropska unija (2010).
Fig.3.
Direktiva 2010/63/EU Evropskega parlamenta in Sveta z dne 22. septembra 2010 o zaščiti živali, ki se uporabljajo v znanstvene namene.
Photograph of fish body showing the cut site when the gonad is fixed in the fixing solution other than 10 % neutral buffered formalin.
UL L 276, 20.10.2010, str. 33.
In that case, the remaining body will be cut off between anterior region of anal fin and anal using razor (red bar), and the head side of fish body will be put into the fixing solution for gonad and the tail side of the fish body will be put into the 10 % neutral buffered formalin.
http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033:0079:SL:PDF
Papillary processes normally appear only in adult males and are found on fin rays from the second to the seventh or eighth counting from the posterior end of the anal fin (Fig.1 and 2).
Poglavje C.2 te priloge: Preskus akutne imobilizacije vodnih bolh (Daphnia sp.)
However, processes rarely appear on the first fin ray from the posterior end of the anal fin.
Series on Testing and Assessment No.
This SOP covers the measurement of processes on the first fin ray (the fin ray number refers to the order from the posterior end of the anal fin in this SOP).
OECD, Pariz.
RECOMMENDED PROCEDURES FOR SAMPLE COLLECTION FOR VITELLOGENIN ANALYSIS
Detailed review paper „Fish embryo toxicity assays“.
Care should be taken to avoid cross-contamination between VTG samples of males and females.
UBA report under contract no.
Procedure 1A:
20385422 German Federal Environment Agency, Berlin.
Fathead Minnow, Blood Collection from the Caudal Vein/Artery
Končna točka na višjem nivoju biološke organizacije
After anaesthetisation, the caudal peduncle is partially severed with a scalpel blade and blood is collected from the caudal vein/artery with a heparinised microhematocrit capillary tube.
povzroča učinke na ravni populacije.
After the blood has been collected, the plasma is quickly isolated by centrifugation for 3 min at 15000 g (or alternatively for 10 min. at 15000 g at 4 °C).
celična tvorba okoli animalnega pola, ki prekriva določen del rumenjaka.
If desired, percent haematocrit can be determined following centrifugation.
snov ali zmes.
The plasma portion is then removed from the microhematocrit tube and stored in a centrifuge tube with 0,13 units of aprotinin (a protease inhibitor) at – 80 °C until determination of VTG can be made.
Epibolija
Depending on the size of the fathead minnow (which is sex-dependent), collectable plasma volumes generally range from 5 to 60 microliters per fish (Jensen et al.
je velika proliferacija zlasti epidermalnih celih v fazi gastrulacije zarodka in njihovo gibanje od dorzalne proti ventralni strani, s katerim se entodermalne celične plasti internalizirajo pri procesu, podobnem invaginaciji, rumenjak pa se vključi v zarodek.
2001).
Pretočni preskus
Procedure 1B:
preskus, pri katerem se testne raztopine med izpostavljenostjo neprekinjeno pretakajo skozi preskusni sistem.
Fathead Minnow, Blood Collection from Heart
Notranja kontrola plošč
Alternatively, blood may also be collected by cardiac puncture using a heparinised syringe (1000 units of heparin per ml).
notranja kontrola, ki jo sestavljajo štiri jamice, napolnjene z vodo za redčenje, na vsaki plošči s 24 jamicami, da se ugotovi, ali je proizvajalec ali raziskovalec med postopkom morda kontaminiral plošče in ali lahko katera koli plošča vpliva na rezultat preskusa (npr. zaradi temperaturnega gradienta).
The blood is transferred into Eppendorf tubes (held on ice) and then centrifuged (5 min, 7000 g, room temperature).
Mednarodna zveza za čisto in uporabno kemijo.
The plasma should be transferred into clean Eppendorf tubes (in aliquots if the volume of plasma makes this feasible) and promptly frozen at – 80 °C, until analysed (Panter et al., 1998).
Voda za vzdrževanje
Procedure 2A:
voda, v kateri poteka oskrba odraslih rib.
Test fish should be removed from the test chamber using the small spoon-net.
Preskus polstatične zamenjave
Be careful not to drop the test fish into other test chambers.
preskus z redno zamenjavo testnih raztopin po določenih obdobjih (npr. vsakih 24 ur).
In principle, the test fish should be removed in the following order: control, solvent control (where appropriate), lowest concentration, middle concentration, highest concentration and positive control.
sistem za poenostavljen zapis strukture molekul.
In addition, all males should be removed from one test chamber before the remaining females are removed.
Somit
The sex of each test fish is identified on the basis of external secondary sex characteristics (e.g. the shape of the anal fin).
v razvijajočem se zarodku vretenčarja so somiti množice mezoderma, ki je razporejen prečno glede na živčno cev, in ki bodo sčasoma razvili usnjico (dermatom), skeletno mišico (miotom) in vretenca (sklerotom).
Place the test fish in a container for transport and carry it to the workstation for excision of the liver.
Statični preskus
If the sex cannot be identified by the fish's external appearance, remove all fish from the test chamber.
vsaka snov ali zmes, preskušena z uporabo te preskusne metode.
In this case, the sex should be identified by observing the gonad or secondary sex characteristics under a stereoscopic microscope.
snovi z neznano ali spremenljivo sestavo, kompleksni reakcijski produkti ali biološki materiali.
Excision of the liver
VZDRŽEVANJE, RAZMNOŽEVANJE IN OBIČAJNI POGOJI ZA PRESKUSE AKUTNE TOKSIČNOSTI ZARODKA CEBRICE
Transfer the test fish from the container for transport to the anaesthetic solution using the small spoon-net.
Cebrica (Danio rerio)
After the test fish is anesthetised, transfer the test fish on the filter paper (or a paper towel) using tweezers (commodity type).
Izvor živalske vrste
When grasping the test fish, apply the tweezers to the sides of the head to prevent breaking the tail.
Indija, Mjanmar, Malaka, Sumatra
Wipe the water on the surface of the test fish on the filter paper (or the paper towel).
Spolni dimorfizem
Then make a small transverse incision partway between the ventral neck region and the mid-abdominal region using dissection scissors.
Samice: izstopajoč trebuh, ko nosijo ikre.
Insert the dissection scissors into the small incision, and incise the abdomen from a point caudal to the branchial mantle to the cranial side of the anus along the midline of the abdomen.
Samci: vitkejši, oranžni odtenek med modrimi vzdolžnimi črtami (opazen zlasti pri predrepni plavuti).
Be careful not to insert the dissection scissors too deeply so as to avoid damaging the liver and gonad.
Suha hrana v kosmičih (največ 3 % teže ribe na dan) 3–5-krat na dan; dodatno navpliji morskih rakcev (vrste Artemia) in/ali majhne vodne bolhe ustrezne velikosti, pridobljene iz nekontaminiranega vira.
Conduct the following operations under the stereoscopic microscope.
S hranjenjem z živo hrano se obogati okolje, zato je treba cebrice hraniti z živo hrano, če je to le mogoče.
Place the test fish abdomen side up on the paper towel (glass Petri dish or slide glass are also available).
Za zagotovitev optimalne kakovosti vode je treba čezmerno količino hrane in iztrebke odstraniti približno eno uro po hranjenju.
Extend the walls of the abdominal cavity with precision tweezers and exteriorise the internal organs.
Približna teža odrasle ribe
It is also acceptable to exteriorise the internal organs by removing one side of the wall of the abdominal cavity if necessary.
Samice: 0,65 ± 0,13 g.
Expose the connected portion of the liver and gallbladder using another pair of precision tweezers.
Samci: 0,5 ± 0,1 g.
Then grasp the bile duct and cut off the gallbladder.
Vzdrževanje starševskih rib
Be careful not to break the gallbladder.
Osvetlitev
Grasp the oesophagus and excise the gastrointestinal tract from the liver in the same way.
Fluorescenčne sijalke (široki spekter), 10–20 μE/m2/s, 540–1080 luksov ali 50–100 ft-c (ravni v laboratoriju); 12–16-urno obdobje osvetljenosti.
Be careful not to leak the contents of the gastrointestinal tract.
26 °C ± 1 °C
Excise the caudal gastrointestinal tract from the anus and remove the tract from the abdominal cavity.
Kakovost vode
Trim the mass of fat and other tissues from the periphery of the liver.
O2 ≥ 80 % nasičenosti, trdota: npr. ~30–300 mg/l CaCO3, NO3-: ≤ 48 mg/l, NH4+ in NO2-: < 0,001 mg/l, preostali klor < 10 μg/l, skupni organski klor < 25 ng/l, pH = 6,5–8,5.
Using the precision tweezers, remove any additional fat and extraneous tissue (e.g. abdominal lining), if needed, from the surface of the liver.
Npr. 180 l, 1 riba/l.
Measure the liver weight with 1,5 ml microtube as a tare using an electronic analytical balance.
Čiščenje vode
Record the value on the worksheet (read: 0,1 mg).
Stalno (prečiščevanje z ogljem), druge možnosti pa so med drugim kombinacija z vzdrževanjem s polstatično zamenjavo ali pretočnim sistemom z neprekinjeno zamenjavo vode.
Close the cap of the microtube containing the liver.
2: 1 (ali množično drstenje).
Store it in a cooling rack (or ice rack).
Akvariji za drstenje
When the livers are donated for pre-treatment shortly after the excision, the specimens are carried to the next workstation in a cooling rack (or ice rack).
Hitrost drstenja
Following liver excision, the fish carcass is available for gonad histology and measurement of secondary sex characteristics.
Ena odrasla samica odloži vsaj 50–80 iker na dan.
Specimen
Hitrost drstenja je lahko bistveno večja, kar je odvisno od vrste.
Store the liver specimens taken from the test fish at ≤ – 70 °C if they are not used for the pre-treatment shortly after the excision.
Stopnja oplojenosti mora znašati ≥ 70 %.
A cut is made just anterior to pectoral fins with scissors
Pri ribah, ki se prvič drstijo, je lahko stopnja oplojenosti iker nižja pri prvih nekaj drstenjih.
The midline of abdomen is incised with scissors to a point approximately 2 mm cranial to the anus
Statični preskus, polstatična zamenjava, pretočni preskus, 26 °C ± 1 °C, preskusne komore, ki so se kondicionirale 24 ur (npr. plošče s 24 jamicami s 2,5–5 ml na jamico)
The abdominal walls are spread with forceps for exposure of the liver and other internal organs
NORMALEN RAZVOJ CEBRIC PRI 26 °C
(Alternatively, the abdominal walls may be pinned laterally).
Slika 1: Izbrane faze zgodnjega razvoja cebric (Danio rerio): 0,2–1,75 ure po oploditvi (na podlagi Kimmel in drugi., 1995 (35)).
Arrow shows liver
Za diagnozo oploditve in sposobnosti preživetja iker se lahko uporabi časovno zaporedje normalnega razvoja (glej odstavek 26: Izbira oplojenih iker).
The liver is bluntly dissected and excised using forceps
Slika 2: Izbrane faze poznega razvoja cebric (Danio rerio) (zarodek, pri katerem se odstrani horij, da se optimizira vidljivost): 22–48 ur po oploditvi (na podlagi Kimmel in drugi., 1995 (35)).
Figure 5
Slika 3: Normalen razvoj zarodkov cebric (Danio rerio): (1) 0,75 ure, faza dveh celic; (2) 1 ura, faza štirih celic; (3) 1,2 ure, faza osmih celic; (4) 1,5 ure, faza šestnajstih celic (5) 4,7 ure, začetek epibolije; (6) 5,3 ure, približno 50 % epibolije (na podlagi Braunbeck in Lammer, 2006 (40)).
The intestines are gently retracted using forceps
Razporeditev plošč s 24 jamicami
Figure 6
1–5 pet preskusnih koncentracij/kemikalijo,
Both ends of the intestines and any mesenteric attachments are severed using scissors
nC negativna kontrola (voda za redčenje),
Figure 7 (female)
iC notranja kontrola plošč (voda za redčenje),
The procedure is identical for the female
pC pozitivna kontrola (4 mg/l 3,4-dikloroanilina),
Figure 8
sC kontrola s topilom
The completed procedure
24 ur predh. kondic. odpadki
Procedure 2 B:
n oplojenih iker na preskusno koncentracojo/ kontrolo Izbira oplojenih iker in določanje stopnje oplojenosti
Japanese Medaka (Oryzias latipes), Liver Pre-treatment for Vitellogenin Analysis
ATLAS SMRTONOSNIH KONČNIH TOČK PRI PRESKUSU AKUTNE TOKSIČNOSTI ZARODKA CEBRICE
Take the bottle of homogenate buffer from the ELISA kit and cool it with crushed ice (temperature of the solution: ≤ 4°C).
Naslednje končne točke na višjem nivoju biološke organizacijo pomenijo akutno toksičnost in posledično smrt zarodka: koagulacija zarodka, odsotnost ločenosti repa, odsotnost oblikovanja somitov in odsotnost srčnega utripa.
If homogenate buffer from EnBio ELISA system is used, thaw the solution at room temperature, and then cool the bottle with crushed ice.
Za prikaz teh končnih točk so bili izbrani mikrografi v nadaljevanju.
Calculate the volume of homogenate buffer for the liver on the basis of its weight (add 50 μl of homogenate buffer per mg liver weight).
Koagulacija zarodka: