natgillin/translations-raw huggingface dataset:
Smaller pools (less than 100 samples):
Pri manjših skupkih (glej oddelek 3II(b)) je treba količino ledu ustrezno zmanjšati.
A digestion fluid is prepared by mixing about one and a half litres of water and 25 ml of 17,5 % hydrochloric acid.
Ohlajena digestivna tekočina se prenese v dvolitrski ločilni lijak, ki je opremljen z vibratorjem, pritrjenim s posebno objemko.
6 g of pepsin is added and the whole mixed at a temperature of 40 to 41 °C.
Sedimentacija lahko poteka 30 minut, medtem pa sedimentacijski lijak prekinjeno vibrira, tj. enominutna vibracija, kateri sledi enominutni premor.
Of the digestion fluid, a volume corresponding to 15 ml per gram of sample is measured (e.g. for 30 samples the volume required is 30 × 15 ml = 450 ml) and transferred to the inner of the two plastic bags, together with the meat samples weighing approximately 1 g (at 25 to 30 °C) taken from each individual sample in accordance with point 2.
Po 30 minutah se 60 ml vzorec sedimenta hitro odplakne v 100 ml merilni valj.
Water at a temperature of approximately 41 °C is poured into the outer bag to make up a total volume in the two bags of one and a half litres.
(Lijak se po uporabi splakne z raztopino detergenta).
The plastic bag is washed with approximately 100 ml of water (at 25 to 30 °C), which is then used to rinse the sieve and lastly added to the filtrate in the beaker.
60 ml vzorec se pusti stati najmanj 10 minut, nato se supernatant odvzame s pipetiranjem, da znaša preostala prostornina 15 ml, ki se pregleda na prisotnost ličink.
Recovery of larvae by sedimentation
Za pipetiranje se lahko uporabi brizgalka za enkratno uporabo, opremljena s plastično cevko.
Ice (300 to 400 g of ice flakes, scaly ice or crushed ice) is added to the digestion fluid to bring its volume up to about 2 litres.
Dolžina cevke mora biti tolikšna, da 15 ml ostane v merilnem valju, ko po pipetiranju brizgalka ostane na obroču valja.
The digestion fluid is then stirred until the ice has melted.
Preostalih 15 ml se odlije v posodo za štetje ličink ali dve petrijevki ter pregleda s trihinoskopom ali stereomikroskopom.
In the case of smaller pools (see Section II(b)), the amount of ice must be reduced correspondingly.
Merilni valj se splakne z 5 do 10 ml vode iz pipe, to pa se doda k vzorcu.
The chilled digestion fluid is transferred to a 2 litre separation funnel, equipped with a vibrator in an extra clamp.
V nobenem primeru se pregled ne sme prestaviti na naslednji dan.
Sedimentation is allowed to proceed for 30 minutes, during which time the sedimentation funnel is vibrated intermittently, i.e. one minute vibration followed by a one-minute pause.
Če so vzorci motni ali pa niso bili pregledani v 30 minutah po njihovi pripravi, jih je treba razbistriti na naslednji način:
After 30 minutes, a 60 ml sample of the sediment is quickly run off into a 100 ml measuring cylinder (the funnel is rinsed with detergent solution after use).
končni vzorec 60 ml se vlije v merilni valj in pusti stati 10 minut;
The 60 ml sample is allowed to stand for at least 10 minutes, after which time the supernatant is withdrawn by suction to leave a volume of 15 ml, to be examined for presence of larvae.
nato se 45 ml supernatanta (bistre tekočine) odvzame s pipetiranjem, preostalim 15 ml pa se dolije voda iz pipe do 45 ml,
For suction, a disposable syringe, equipped with a plastic tube, can be used.
po nadaljnjem usedanju, ki traja 10 minut, se 30 ml supernatanta (bistre tekočine) odvzame s pipetiranjem, preostalih 15 ml za pregled pa se vlije v petrijevko ali posodo za štetje ličink,
The length of the tube must be such that 15 ml remains in the measuring cylinder when the flanges of the syringe rest on the cylinder's rim.
merilni valj se splakne z 10 ml vode iz pipe, to pa se skupaj doda k vzorcu v petrijevki ali posodi za štetje ličink, ki je pripravljen za pregled.
The remaining 15 ml is poured into a larval counting basin or two petri dishes and examined using a trichinoscope or stereo-microscope.
Če je rezultat pozitiven ali nejasen, se uporabljajo določbe iz poglavja I(3)(III).
The measuring cylinder is washed with 5 to 10 ml of tap water and the washings are added to the sample.
B. Mehanska metoda prebave zbirnega vzorca/tehnika „izolacije na filtru“
Under no circumstances is examination to be postponed until the following day.
Kot je določeno v oddelku A(1).
Where the digests are unclear or they are not examined within 30 minutes of their preparation, they must be clarified as follows:
Dodatna oprema:
the final sample of 60 ml is poured into a measuring cylinder and allowed to stand for 10 minutes;
itrski Gelman lijak skupaj z držalom za filter (premer 45 mm);
45 ml of supernatant fluid is then removed by suction and the remaining 15 ml is made up to 45 ml with tap water,
filtrirni diski, ki so sestavljeni iz okrogle mreže iz nerjavečega jekla z odprtino 35 mikronov (premer diska: 45 mm), dveh gumijastih obročev, debelih 1 mm, (zunanji premer: 45 mm, notranji premer: 38 mm), okrogla mreža se položi med oba gumijasta obroča in nanju pritrdi z uporabo dvokomponentnega lepila, ki je primerno za oba materiala;
after a further settling period of 10 minutes, 30 ml of supernatant fluid is removed by suction and the remaining 15 ml is poured into a petri dish or larval counting basin for examination,
erlenmajerica s prostornino treh litrov, ki je opremljena s stransko cevko za pipetiranje;
the measuring cylinder is washed with 10 ml of tap water and these washings are added to the sample in the petri dish or the larval counting basin for examination.
filtrirna črpalka;
Where the result is positive or uncertain, the provisions laid down in Chapter I(3)(III) shall apply.
plastične vrečke s prostornino najmanj 80 ml;
B. Mechanically assisted pooled sample digestion method/'on filter isolation' technique
oprema za zapiranje plastičnih vrečk;
As stipulated in Section A(1).
rennila se z močjo 1:150000 Soxhlet enot na gram.
Additional equipment:
Odvzem vzorcev
1 litre Gelman funnel, complete with filter holder (diameter 45 mm);
Popolni skupki (100 vzorcev hkrati)
filter discs, consisting of a circular stainless steel mesh with an aperture of 35 microns (disc diameter: 45 mm), two rubber rings 1 mm thick (external diameter: 45 mm; internal diameter: 38 mm), the circular mesh being placed between the two rubber rings and bonded to them using a two-component glue suitable for the two materials;
Glej oddelek A(3)(II)(a).
an Erlenmeyer flask, capacity 3 litres, fitted with a side tube for suction;
Manjši skupki (manj kot 100 vzorcev)
a filter pump;
Glej oddelek A(3)(II)(b).
plastic bags, capacity at least 80 ml;
Ugotavljanje prisotnosti ličink s filtracijo
equipment for sealing the plastic bags;
Pri manjših skupkih je treba količino ledu ustrezno zmanjšati.
rennilase, strength 1:150000 Soxhlet units per gram.
Digestivna tekočina se meša, dokler se led ne raztopi.
Collecting of specimens
Ohlajeno digestivno tekočino se nato pusti stati najmanj tri minute, da se ličinke zvijejo.
Complete pools (100 samples at a time)
Gelman lijak, opremljen z držalom za filter in filtrirnim diskom, se pritrdi na erlenmajerico, ki je povezana s filtrirno črpalko.
See Section A(3)(II)(a).
Digestivna tekočina se vlije v Gelman lijak in prefiltrira.
Smaller pools (less than 100 samples)
Proti koncu filtracije se lahko pretok digestivne tekočine skozi filter olajša z uporabo filtrirne črpalke.
See Section A(3)(II)(b).
Pipetiranje se mora zaključiti, preden se filter osuši, to pomeni, ko v lijaku ostane 2 do 5 ml tekočine.
Recovery of larvae by filtration
Ko je vsa digestivna tekočina prefiltrirana, se filtrirni disk odstrani in položi v plastično vrečko s prostornino 80 ml skupaj s 15 do 20 ml rennilase raztopine.
In the case of smaller pools, the amount of ice must be reduced correspondingly.
Rennilase raztopina se naredi tako, da se 2 g rennilase doda k 100 ml vode iz pipe.
The digestion fluid is stirred until the ice has melted.
Plastična vrečka se dvakrat zapečati in vloži v Stomacher med notranjo in zunanjo vrečko.
The chilled digestion fluid is then left for at least three minutes to let the larvae coil.
Stomacher naj melje tri minute, npr. popoln ali nepopoln vzorec.
The Gelman funnel, fitted with a filter holder and filter disc, is mounted on an Erlenmeyer flask connected to a filter pump.
Po treh minutah se plastična vrečka skupaj s filtrirnim diskom in rennilase raztopino odstrani iz Stomacherja in odpre s škarjami.
The digestion fluid is poured into the Gelman funnel and filtered.
Tekoča vsebina se vlije v posodo za štetje ličink ali petrijevko.
Towards the end of filtration, the digestion fluid can be helped to pass through the filter by applying suction with the filter pump.
Vrečka se splakne s 5 do 10 ml vode, ki se nato doda v posodo za štetje ličink za pregled pod trihinoskopom ali v petrijevko za pregled s stereomikroskopom.
Suction must cease before the filter becomes dry, i.e. when 2 to 5 ml of fluid is left in the funnel.
Vzorci se morajo pregledati takoj, ko so pripravljeni.
Once all the digestion fluid has been filtered, the filter disc is removed and placed in an 80 ml capacity plastic bag, together with 15 to 20 ml of rennilase solution.
Opomba:
The rennilase solution is made by adding 2 g of rennilase to 100 ml of tap water.
Filtrirni diski se ne smejo uporabljati, če niso popolnoma čisti.
The plastic bag is sealed twice and placed between the inner and outer bags in the Stomacher.
Diski se nikoli ne smejo posušiti v nečistem stanju.
The Stomacher is allowed to pound for three minutes, e.g. while it is working on a complete or incomplete pool.
Filtrirni diski se lahko očistijo tako, da se čez noč pustijo v rennilase raztopini.
After three minutes, the plastic bag, complete with filter disc and rennilase solution, is removed from the Stomacher and opened with scissors.
Pred uporabo jih je treba očistiti v sveži rennilase raztopini z uporabo Stomacherja.
The liquid contents are poured into a larval counting basin or petri dish.
C. Avtomatska metoda umetne prebave zbirnih vzorcev do 35 g
The bag is washed out with 5 to 10 ml of water, which is then added to the larval counting basin for examination by trichinoscope or to the petri dish for examination by stereo-microscope.
mešalec Trichomatic 35® s filtrskim vložkom;
Digests must be examined as soon as they are ready.
klorovodikova kislina z 8,5 % ± 0,5 % maso;
Note:
prozorni polikarbonatni membranski filtri s premerom 50 mm in velikostjo pore 14 mikronov;
Filter discs must never be used when not completely clean.
pepsin z močjo 1:10000 NF (US National Formulary), kar ustreza 1:12500 BP (British Pharmacopoeia) in 2000 FIP (Fédération internationale de pharmacie), ali stabilizirana raztopina pepsina z najmanj 660 enot Evropske farmakopeje/ml;
Unclean discs must never be allowed to dry out.
pinceta s ploščato konico;
Filter discs can be cleaned by leaving them in rennilase solution overnight.
objektna stekelca za mikroskopiranje z dolžino stranice najmanj 5 cm ali petrijevke s premerom najmanj 6 cm, katerih dno je s koničastim instrumentom razdeljeno na kvadrate velikosti 10 × 10 mm;
Before use, they must be washed in fresh rennilase solution using the Stomacher.
nastavljiv (stereo-) mikroskop (15 do 60-kratna povečava) ali trihinoskop s horizontalno ploščo;
C. Automatic digestion method for pooled samples of up to 35 g
posoda za zbiranje tekočih odpadkov;
A Trichomatic 35® blender with filtration insert.
I. Postopek prebave
Hydrochloric acid 8,5 ± 0,5 % weight.
Namestite mešalec s filtrirnim vložkom, povežite odvodno cevko in jo napeljite v posodo za zbiranje tekočih odpadkov.
Transparent polycarbonate membrane filters with a diameter of 50 mm and a pore size of 14 microns.
Po vključitvi mešalca se začne segrevanje.
Pepsin, strength 1:10000 NF (US National Formulary) corresponding to 1:12500 BP (British Pharmacopoeia) and to 2000 FIP (Fédération internationale de pharmacie), or stabilised liquid pepsin with minimum 660 European Pharmacopoeia units/ml.
Pred tem je treba odpreti in zapreti spodnji ventil, ki se nahaja pod reakcijsko komoro.
Tweezers with a flat tip.
Nato se doda največ 35 vzorcev po približno 1 g (pri 25 do 30 °C), ki so bili vzeti od posameznih vzorcev v skladu s točko 2.
A number of microscope slides with a side-length of at least 5 cm or a number of petri dishes at least 6 cm in diameter, marked on their undersides into 10 × 10 mm square areas using a pointed instrument.
Preverite, da so odstranjeni večji kosi kit, ker lahko zamašijo membranski filter.
A (stereo-)microscope with transmitted light (magnification 15 to 60 times) or a trichinoscope with a horizontal table.
Nalijte vodo do roba komore za tekočino, povezane z mešalcem (približno 400 ml).
A bin for collection of waste liquids.
Nalijte okrog 30 ml klorovodikove kisline (8,5 %) do roba manjše komore za tekočino, ki je povezana z mešalcem.
I. Digestion procedure
Pod grobi filter v držalu filtrirnega vložka namestite membranski filter.
Place the blender with the filtration insert, connect the waste tube and place the tube so it drains into the waste bin.
Na koncu dodajte 7 g pepsina ali 21 ml raztopine pepsina.
When the blender is switched on, heating will start.
Zaprite pokrova reakcijske komore in komore za tekočino.
Before this is done, the bottom valve located below the reaction chamber must be opened and closed.
Nastavite čas prebave.
Up to 35 samples weighing approximately 1 g each (at 25 to 30 °C) taken from each individual sample in accordance with point 2 are then added.
Kratek čas prebave (5 minut) je treba nastaviti za prašiče običajne starosti za zakol in podaljšani čas prebave (8 minut) za druge vzorce.
Ensure that larger pieces of tendons are removed as they may clot the membrane filter.
Z vklopom gumba za start na mešalcu se avtomatsko začne postopek dispenzacije in prebave, nato sledi filtriranje.
Pour water up to the edge of a liquid chamber connected to the blender (approximately 400 ml).
Po 10 do 13 minutah je postopek končan in mešalec se avtomatsko ustavi.
Pour about 30 ml hydrochloric acid (8,5 %) to the edge of the smaller, connected liquid chamber.
Ko ste preverili, ali je reakcijska komora prazna, odprite njen pokrov.
Place a membrane filter under the coarse filter in the filter holder in the filter insert.
Če so v komori pena ali ostanki digestivne tekočine, ponovite postopek v skladu z oddelkom V.
Lastly, add 7 g of pepsin or 21 ml liquid pepsin.
Ugotavljanje prisotnosti ličink
Close the lids of the reaction and liquid chambers.
Odstranite držalo filtra in prenesite membranski filter na objektno stekelce ali petrijevko.
Select the period of digestion.
Preglejte membranski filter s (stereo) mikroskopom ali trihinoskopom.
A short digestion period (5 minutes) must be set for pigs at the normal slaughter age and a longer time (8 minutes) for other samples.
Čiščenje opreme
When the start button on the blender is turned on, the process of dispensing and digestion starts automatically, followed by filtration.
Če je rezultat pozitiven, napolnite 2/3 reakcijske komore v mešalcu z vrelo vodo.
After 10 to 13 minutes the process is completed and stops automatically.
Komoro za tekočino napolnite z vodo iz pipe do ravni spodnjega kazalca.
Open the lid of the reaction chamber after checking that the chamber is empty.
Nato se začne avtomatsko čiščenje.
If there is foam or any digestion liquid remaining in the chamber, repeat the procedure in accordance with Section V.
Razkužite držalo filtra in preostalo opremo, na primer z uporabo formalina.
Recovery of larvae
Pred koncem delavnika napolnite komoro za tekočino v mešalcu z vodo in izvedite standardni program.
Remove the filter holder and transfer the membrane filter to a slide or petri dish.
Uporaba membranskih filtrov
Examine the membrane filter using a (stereo-)microscope or a trichinoscope.
Vsak polikarbonatni membranski filter se lahko uporabi največ petkrat.
Cleaning equipment
Filter je treba po vsaki uporabi obrniti.
Where the result is positive, fill the blender reaction chamber with boiling water until it is two-thirds full.
Poleg tega je treba po vsaki uporabi preveriti, ali je filter morda poškodovan, zaradi česar ne bi bil več primeren za nadaljnjo uporabo.
Ordinary tap water is poured into the connecting liquid chamber until it covers the lower sensor.
V. Metoda, ki se uporabi, kadar prebava ni popolna in zato ni mogoče opraviti filtracije
Automatic cleaning then takes place.
Ko ste v mešalcu izvedli avtomatski postopek v skladu z oddelkom I, odprite pokrov reakcijske komore in preverite, ali je v komori ostala pena ali tekočina.
Decontaminate the filter-holder and any other equipment, e.g. using formol.
Če je tako, opravite naslednji postopek:
After work is completed for the day, fill the blender liquid chamber with water and put it through a standard cycle.
Zaprite spodnji ventil pod reakcijsko komoro.
Use of membrane filters
Odstranite držalo filtra in prenesite membranski filter na objektno stekelce ali petrijevko.
Each polycarbonate membrane filter may be used no more than five times.
Vložite nov membranski filter v držalo filtra in pritrdite držalo filtra.
The filter is to be turned between each use.
Komoro mešalca za tekočino napolnite z vodo do ravni spodnjega kazalca.
In addition, the filter must be checked after each use for any damage which would make it unsuitable for further use.
Izvedite avtomatski program čiščenja.
V. Method to be applied when digestion is incomplete and filtration cannot be carried out
Ko je program čiščenja končan, odprite pokrov reakcijske komore in preverite, ali je v komori še kaj tekočine.
Once the blender has been put through an automatic cycle in accordance with Section I, open the lid of the reaction chamber and check whether there is foam or any liquid remaining in the chamber.
Če je komora prazna, odstranite držalo filtra in s pinceto prenesite membranski filter na objektno stekelce ali petrijevko.
If this is the case, proceed as follows:
Oba membranska filtra preglejte v skladu z oddelkom II.
close the bottom valve below the reaction chamber;
Če filtrov ni mogoče pregledati, ponovite celoten postopek prebave s podaljšanim časom prebave v skladu z oddelkom I.
remove the filter holder and transfer the membrane filter to a slide or petri dish;
D. Metoda prebave zbirnega vzorca z magnetnim mešanjem/„izolacijo na filtru“ in odkrivanjem ličink s testom lateksne aglutinacije
put a new membrane filter in the filter holder and attach the filter holder;
Ta metoda se šteje kot enakovredna le za testiranje mesa domačih prašičev.
fill the blender liquid chamber with water until the lower sensor is covered;
jeklena naprava za filtriranje s filtri z 20-mikronsko mrežo z jeklenim lijem;
carry out the automatic cleaning cycle;
vakuumska črpalka;
after the cleaning cycle has ended, open the lid of the reaction chamber and check whether any liquid remains;
kovinske ali plastične posode z 10- do 15-litrsko prostornino za zbiranje digestivne tekočine;
if the chamber is empty, remove the filter holder and transfer the membrane filter to a slide or petri dish with tweezers;
rotacijski stresalnik;
examine the two membrane filters in accordance with Section II.
pipete različnih velikosti (1, 10 in 25 ml), mikropipete v skladu z navodili proizvajalca glede lateksne aglutinacije in držala za pipete;
If the filters cannot be examined, repeat the entire digestion process with a longer digestion time in accordance with Section I.
filtri z 20-mikronsko najlonsko mrežo, premera, ki ustreza filtracijskemu sistemu;
D. Magnetic stirrer method for pooled sample digestion/'on filter isolation' and larva detection by a latex agglutination test
plastične ali jeklene klešče velikosti 10 do 15 cm;
This method is only considered equivalent for the testing of meat of domestic swine.
koničaste epruvete s prostornino 15 ml;
Steel filtration apparatus for 20 μm mesh filters with a steel funnel.
pestilo s teflonsko ali jekleno stožčasto konico, ki ustreza koničastim epruvetam;
Vacuum pump.
kartice za lateksno aglutinacijo iz kompleta za dokaz antigena Trichin-L, potrjenega pod oznako št.
Metal or plastic tanks, capacity 10 to 15 litres, to collect the digestive juice.
EURLP_D_001/2011;
A 3D gyratory rocker.
puferska raztopina s konzervansom (vzorec razredčila) iz kompleta za dokaz antigena Trichin-L, potrjenega pod oznako št.
Pipettes of different sizes (1, 10 and 25 ml), micropipettes according to the latex agglutination manufacturer's instructions and pipette holders.
EURLP_D_001/2011;
20 microns nylon mesh filters of a diameter that fits with the filtration system.
puferska raztopina s konzervansom (negativna kontrola) iz kompleta za dokaz antigena Trichin-L, potrjenega pod oznako št.
Plastic or steel forceps of 10 to 15 cm.
EURLP_D_001/2011;
Conical vials of 15 ml.
puferska raztopina z antigeni Trichinella spiralis in konzervansom (pozitivna kontrola) iz kompleta za dokaz antigena Trichin-L, potrjenega pod oznako št.
A pestle with a Teflon or steel conical tip to fit in the conical vials.
EURLP_D_001/2011;
Latex agglutination cards of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.
puferska raztopina s polistirenskimi delci, prekritimi s protitelesi, in konzervansom (kroglice iz lateksa) iz kompleta za dokaz antigena Trichin-L, potrjenega pod oznako št.
Buffer solution with preservative (sample diluent) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.
EURLP_D_001/2011;
Buffer supplemented with preservative (negative control) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.
(aa) paličice za enkratno uporabo.
Buffer supplemented with Trichinella spiralis antigens and preservative (positive control) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.
I. Pri popolnih skupkih (100 g vzorca hkrati)
Buffer with polystyrene particles coated with antibodies supplemented with preservative (latex beads) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.
16 ± 0,5 ml 25-odstotne klorovodikove kisline (končna vrednost 0,2 %) se vlije v trilitrsko čašo, ki vsebuje 2,0 litra ± 200 ml vode iz pipe, ki je bila predhodno segreta na 46 °C do 48 °C;
(aa) Disposable sticks.
doda se 10 ± 1 g pepsina v prahu (ali 30 ± 3 ml tekočega pepsina);
I. For complete pools (100 g of samples at a time)
100–115 g vzorcev, odvzetih v skladu s točko 2, se seseklja v mešalcu, skupaj s 150 ml ± 15 ml predhodno segretega digestivnega pufra;
16 ± 0,5 ml of 25 % hydrochloric acid (0,2 % final) is added to a 3 litre beaker containing 2,0 litres ± 200 ml of tap water, preheated to 46 to 48 °C;
na stojalo za filtriranje se postavi filter z 20-mikronsko najlonsko mrežo.
10 ± 1 g of powder pepsin (or 30 ± 3 ml of liquid pepsin) is added.
Stožčasti jekleni lijak za filtriranje se namesti na stojalo s sistemom za blokiranje, jekleno sito s 180-mikronsko mrežo pa se postavi na lijak.
100-115 g of samples collected in accordance with point 2 are chopped in the blender, with 150 ± 15 ml of preheated digestion buffer.
Vakuumska črpalka se poveže s stojalom za filtriranje ter s kovinsko ali plastično posodo za zbiranje digestivne tekočine;
The 20 microns nylon mesh filter is placed on the filtration support.
preneha se z mešanjem, digestivna tekočina pa se preko sita vlije v lijak za filtriranje.
The conical filtration steel funnel is fixed to the support with the block system and the steel sieve of 180 microns mesh size is placed on the funnel.
Čaša se splakne s približno 250 ml tople vode.
The vacuum pump is connected with the filtration support and with the metal or plastic tank, to collect the digestive fluid.
Ko se digestivna tekočina uspešno filtrira, je treba tekočino za izpiranje vliti v filtracijsko enoto;
Stirring is stopped and the digestion fluid is poured into the filtration funnel through the sieve.
s kleščami se prime rob filtracijske membrane.
The beaker is rinsed with approximately 250 ml of warm water.
Filtracijska membrana se najmanj štirikrat prepogne in vloži v 15-mililitrsko stožčasto cev.
The rinsing liquid is poured into the filtration ramp after the digested fluid has been successfully filtrated.
Stožčasta cev se izbere glede na pestilo;