natgillin/translations-raw huggingface dataset:
Directive 2003/99/EC of the European Parliament and of the Council of 17 November 2003 on the monitoring of zoonoses and zoonotic agents, amending Council Decision 90/424/EEC and repealing Council Directive 92/117/EEC (OJ L 325, 12.12.2003, p. 31).
pladnji, razdeljeni na 50 kvadratov, od katerih vsak lahko drži vzorec po približno 2 g mesa, ali druga orodja, ki zagotavljajo enaka jamstva v zvezi s sledljivostjo vzorcev;
ANNEX I
mešalec z ostrim rezilom za sekljanje.
Detection methods
Če so vzorci težji od 3 g, je treba uporabiti strojček za mletje mesa z luknjami premera med 2 in 4 mm ali škarje.
REFERENCE METHOD OF DETECTION
V primeru zamrznjenega mesa ali jezika (po odstranitvi površinske plasti, ki ni prebavljiva) je potreben strojček za mletje mesa, velikost vzorca pa je treba znatno povečati;
Magnetic stirrer method for pooled sample digestion
magnetni mešalci s termostatsko nadzorovano grelno ploščo in približno 5 cm dolgimi teflonskimi mešalnimi paličicami;
Apparatus and reagents
konični stekleni ločilni lijaki s prostornino najmanj 2 litrov, po možnosti opremljeni s teflonskimi varnostnimi čepi;
Knife or scissors and tweezers for cutting specimens.
Stojala, vijaki in objemke.
Trays marked off into 50 squares, each of which can hold samples of approximately 2 g of meat, or other tools giving equivalent guarantees as regards the traceability of the samples.
sita s 180-mikronsko mrežo iz nerjavečega jekla in zunanjim premerom 11 cm;
A blender with a sharp chopping blade.
Lijaki z notranjim premerom najmanj 12 cm za podporo sit.
Where the samples are larger than 3 g, a meat mincer with openings of 2 to 4 mm or scissors must be used.
steklene čaše s 3-litrsko prostornino;
In the case of frozen meat or tongue (after removal of the superficial layer, which cannot be digested), a meat mincer is necessary and the sample size will need to be increased considerably.
stekleni merilni valji s prostornino od 50 do 100 ml ali centrifugalne epruvete;
Magnetic stirrers with thermostatically controlled heating plate and Teflon-coated stirring rods approximately 5 cm long.
trihinoskop s horizontalno ploščo ali stereomikroskop z nastavljivim svetlobnim virom;
Conical glass separation funnels, capacity of at least 2 litres, preferably fitted with Teflon safety plugs.
več petrijevk s premerom 9 cm (za uporabo s stereomikroskopom), katerih spodnja stran se s koničastim orodjem označi v kvadratna preiskovalna polja po 10 × 10 mm;
Stands, rings and clamps.
posoda za štetje ličink (za uporabo s trihinoskopom), ki je narejena iz 3 mm debelih akrilnih plošč, in sicer:
Sieves, mesh size 180 microns, external diameter 11 cm, with stainless steel mesh.
dno posode mora znašati 180 × 40 mm, razdeljeno na kvadrate;
Funnels, internal diameter not less than 12 cm, to support the sieves.
daljše stranice morajo biti 230 × 20 mm;
Glass beakers, capacity 3 litres.
krajše stranice morajo biti 40 × 20 mm.
Glass measuring cylinders, capacity 50 to 100 ml, or centrifuge tubes.
Dno in krajše stranice morajo biti vstavljene med daljše stranice tako, da tvorijo posodo z dvema ročicama na obeh koncih.
A trichinoscope with a horizontal table or a stereo-microscope, with a substage transmitted light source of adjustable intensity.
Zgornji del dna se mora dvigniti za 7 do 9 mm od dna ogrodja, ki ga tvorijo daljše in krajše stranice.
A number of 9 cm diameter petri dishes (for use with a stereo-microscope), marked on their undersides into 10 × 10 mm square examination areas using a pointed instrument.
Sestavne dele je treba zlepiti skupaj z lepilom, primernim za ta material;
A larval counting basin (for use with a trichinoscope), made of 3 mm thick acrylic plates as follows:
aluminijasta folija;
the bottom of the basin to be 180 × 40 mm, marked off into squares,
25 % klorovodikova kislina;
the sides to be 230 × 20 mm,
voda iz pipe, segreta na 46 do 48 °C;
the end to be 40 × 20 mm.
tehtnica z natančnostjo vsaj 0,1 g;
The bottom and the ends must be inserted between the sides, to form two small handles at the ends.
kovinski pladnji s prostornino od 10 do 15 litrov za zbiranje odvečne digestivne tekočine;
The upper side of the bottom must be raised 7 to 9 mm from the base of the frame formed by the sides and the ends.
pipete različnih velikosti (1, 10 in 25 ml) in držala za pipete;
The components must be stuck together with glue suitable for the material.
termometer z natančnostjo 0,5 °C v območju od 1 do 100 °C;
Aluminium foil.
sifon za vodo iz pipe.
Pepsin, strength: 1:10000 NF (US National Formulary) corresponding to 1:12500 BP (British Pharmacopoeia) and to 2000 FIP (Fédération internationale de pharmacie), or stabilised liquid pepsin with minimum 660 European Pharmacopoeia units/ml.
Pri celih trupih domačih prašičev je treba iz preponskega stebra pri prehodu v mišični del vzeti vsaj 1 g vzorca.
Tap water heated to 46 to 48 °C.
Uporabijo se lahko posebne klešče za odvzem vzorca za preiskavo na trihinele, pod pogojem, da se lahko zagotovi natančnost med 1,00 in 1,15 g.
A balance accurate to at least 0,1 g.
Pri plemenskih svinjah in merjascih je treba iz preponskega stebra pri prehodu v mišični del vzeti večji vzorec, ki tehta vsaj 2 g.
Metal trays, capacity 10 to 15 litres, to collect the remaining digestive juice.
Če ni preponskih stebrov, se enkrat večji vzorec, kot sta 2 g (ali 4 g pri plemenskih svinjah in merjascih), vzame iz rebrnega dela ali prsnega dela prepone, iz žvekalke, jezične mišice ali trebušnih mišic.
Pipettes of different sizes (1, 10 and 25 ml) and pipette holders.
Od vsakega kosa mesa se vzame vsaj 5 g težek vzorec prečno progaste mišice z malo maščobe, če je mogoče, v bližini kosti ali kit.
A thermometer accurate to 0,5 °C within the range 1 to 100 °C.
Vzorec iste velikosti se vzame od mesa, za katerega ni predvideno temeljito kuhanje ali kakršna koli druga vrsta predelave po zakolu.
Siphon for tap water.
Pri zamrznjenih vzorcih je treba za analizo vzeti vsaj 5 g težek vzorec tkiva prečno progaste mišice.
Collecting of specimens and quantity to be digested
Teža vzorcev mesa velja za vzorec mesa, ki je brez maščob in fascij.
In the case of whole carcasses of domestic swine, a specimen weighing at least 1 g is to be taken from a pillar of the diaphragm at the transition to the sinewy part.
Posebno pozornost je treba nameniti jemanju vzorcev mišic iz jezika, da se prepreči okužba s površinsko plastjo jezika, ki ni prebavljiva in lahko onemogoči pregled sedimenta.
Special trichinae forceps can be used provided an accuracy of between 1,00 and 1,15 g can be guaranteed.
Postopek
In the case of breeding sows and boars, a larger sample weighing at least 2 g is to be taken from a pillar of the diaphragm at the transition to the sinewy part.
I. Popolni skupki (100 g vzorcev hkrati)
In the absence of diaphragm pillars, a specimen of twice the size 2 g (or 4 g in the case of breeding sows and boars) is to be taken from the rib part or the breastbone part of the diaphragm, or from the jaw muscle, tongue or abdominal muscles.
16 ± 0,5 ml klorovodikove kisline se vlije v trilitrsko čašo, ki vsebuje 2,0 litra vode iz pipe, ki je bila predhodno segreta na 46 do 48 °C;
For cuts of meat, a sample weighing at least 5 g of striated muscle, containing little fat is to be taken, where possible from close to bones or tendons.
mešalna paličica se vstavi v čašo, čaša pa se postavi na predhodno segreto ploščo in postopek mešanja se začne;
A sample of the same size is to be collected from meat that is not intended to be cooked thoroughly or other types of post-slaughter processing.
Doda se 10 ± 0,2 g pepsina ali 30 ± 0,5 ml raztopine pepsina.
For frozen samples, a sample weighing at least 5 g of striated muscle tissue is to be taken for analysis.
100 g vzorcev, vzetih v skladu s točko 2, se seseklja v mešalcu.
The weight of meat specimens relates to a sample of meat that is free of all fat and fascia.
sesekljano meso se prenese v trilitrsko čašo, ki vsebuje vodo, pepsin in klorovodikovo kislino;
Special attention must be paid when collecting muscle samples from the tongue in order to avoid contamination with the superficial layer of the tongue, which is indigestible and can prevent reading of the sediment.
rezilni nastavek mešalca se večkrat potopi v digestivno tekočino v čaši, posoda mešalca pa se splakne z manjšo količino digestivne tekočine, da se odstrani vse meso, ki se še drži;
Procedure
čaša se pokrije z aluminijasto folijo;
I. Complete pools (100 g of samples at a time)
magnetni mešalec je treba nastaviti tako, da med delovanjem vzdržuje stalno temperaturo med 44 °C in 46 °C.
16 ± 0,5 ml of hydrochloric acid is added to a 3 litre beaker containing 2,0 litre of tap water, preheated to 46 to 48 °C;
Med mešanjem mora digestivna tekočina krožiti dovolj hitro, da ustvari globok vrtinec brez pljuskanja;
a stirring rod is placed in the beaker, the beaker is placed on the preheated plate and the stirring is started.
digestivna tekočina se meša, dokler mesni delci ne izginejo (približno 30 minut).
10 ± 0,2 g of pepsin or 30 ± 0,5 ml liquid pepsin is added.
Nato se mešalec izklopi in digestivna tekočina se skozi sito vlije v sedimentacijski lijak.
100 g of samples collected in accordance with point 2 is chopped in the blender.
Pri obdelavi nekaterih vrst mesa (jezik, divjačinsko meso itd.) je lahko potreben daljši čas digestije (a ne več kot 60 minut);
The chopped meat is transferred to the 3 litre beaker containing the water, pepsin and hydrochloric acid.
digestivni postopek se šteje za zadovoljivega, če na situ ne ostane več kot 5 % začetne teže vzorca;
The mincing insert of the blender is immersed repeatedly in the digestion fluid in the beaker and the blender bowl is rinsed with a small quantity of digestion fluid to remove any meat still adhering.
Digestivna tekočina se pusti stati v lijaku 30 minut.
The beaker is covered with aluminium foil.
Po 30 minutah se vzorec digestivne tekočine (40 ml) hitro odplakne v merilni valj ali centrifugalno cevko.
The magnetic stirrer must be adjusted so that it maintains a constant temperature of 44 to 46 °C throughout the operation.
Digestivne tekočine in drugi tekoči odpadki se obdržijo na pladnju, dokler se odčitavanje rezultatov ne konča.
During stirring, the digestion fluid must rotate at a sufficiently high speed to create a deep whirl without splashing.
40 ml vzorca se sme pustiti stati 10 minut.
The digestion fluid is stirred until the meat particles disappear (approximately 30 minutes).
Nato se s pipetiranjem previdno odvzame 30 ml supernatanta (bistre tekočine), da se odstranijo zgornji sloji do prostornine največ 10 ml.
The stirrer is then switched off and the digestion fluid is poured through the sieve into the sedimentation funnel.
Preostalih 10 ml vzorca sedimenta se vlije v posodo za štetje ličink ali petrijevko.
Longer digestion times may be necessary (not exceeding 60 minutes) in the processing of certain types of meat (tongue, game meat, etc.).
Merilni valj ali centrifugalna epruveta se splakne z največ 10 ml vode iz pipe, to pa se skupaj doda vzorcu v posodi za štetje ličink ali petrijevki.
The digestion process is considered satisfactory if not more than 5 % of the starting sample weight remains on the sieve.
Vzorec se nato preuči s trihinoskopom ali stereomikroskopom pri 15- do 20-kratni povečavi.
The digestion fluid is allowed to stand in the funnel for 30 minutes.
Ponazoritev z uporabo drugih tehnik je dovoljena, če se je izkazalo, da preiskava pozitivnih kontrolnih vzorcev poda enak ali boljši rezultat od tradicionalnih metod ponazoritve.
After 30 minutes, a 40 ml sample of digestion fluid is quickly run off into the measuring cylinder or centrifuge tube.
Pri vseh primerih sumljivih delov ali oblik, podobnih parazitom, je treba uporabiti večjo povečavo (od 60- do 100-kratno).
The digestion fluids and other liquid waste are kept in a tray until reading of the results is completed.
Vzorci se morajo pregledati takoj, ko so pripravljeni.
The 40 ml sample is allowed to stand for 10 minutes.
V nobenem primeru se pregled ne sme prestaviti na naslednji dan.
30 ml of supernatant is then carefully withdrawn by suction to remove the upper layers and leave a volume of not more than 10 ml.
Če vzorci niso pregledani v 30 minutah po pripravi, jih je treba razbistriti na naslednji način.
The remaining 10 ml sample of sediment is poured into a larval counting basin or petri dish.
Približno 40 ml končnega vzorca se vlije v merilni valj in pusti stati 10 minut.
The cylinder or centrifuge tube is rinsed with not more than 10 ml of tap water, which has to be added to the sample in the larval counting basin or petri dish.
Nato se odvzame 30 ml supernatanta (bistre tekočine) do prostornine 10 ml.
Subsequently, the sample is examined by trichinoscope or stereo-microscope at a 15 to 20 times magnification.
Tej se do prostornine 40 ml doda voda iz pipe.
Visualisation using other techniques is allowed, provided examination of positive control samples has been shown to give an equal or better result than traditional visualisation methods.
Če je sediment pri pregledu moten, se vzorec vlije v merilni valj in doda se voda iz pipe do 40 ml, nato pa se ravna v skladu s postopkom opisanem v tem oddelku.
In all cases of suspect areas or parasite-like shapes, higher magnifications of 60 to 100 times must be used.
Ta postopek se lahko ponovi 2- do 4-krat, dokler ni tekočina dovolj čista za zanesljivo odčitavanje.
Digests are to be examined as soon as they are ready.
Skupki z manj kot 100 g
Under no circumstances should examination be postponed until the following day.
Če je potrebno, se lahko k 100 g skupka doda 15 g, ki se pregleda skupaj s temi vzorci v skladu z oddelkom 3(I).
Where the digests are not examined within 30 minutes of preparation, they must be clarified as follows.
V primeru več kot 15 g se to pregleda kot samostojni skupek.
The final sample of about 40 ml is poured into a measuring cylinder and allowed to stand for 10 minutes.
Pri skupkih do 50 g se lahko digestivno tekočino in sestavine zmanjša na 1 liter vode, 8 ml klorovodikove kisline in 5 g pepsina.
30 ml of the supernatant fluid is then removed, leaving a volume of 10 ml.
Pozitivni ali nejasni rezultati
After a further settling period of 10 minutes, 30 ml of the supernatant fluid is withdrawn by suction, leaving a volume of no more than 10 ml for examination in a petri dish or larval counting basin.
Vzorci po 20 g, vzeti petim prašičem, se združijo in pregledajo po v tem poglavju opisani metodi.
The measuring cylinder is washed with no more than 10 ml of tap water and these washings are added to the sample in the petri dish or the larval counting basin for examination.
Na ta način bodo pregledani vzorci 20 skupin po pet prašičev.
If the sediment is found to be unclear on examination, the sample is poured into a measuring cylinder and made up to 40 ml with tap water and then the procedure described in this Section is followed.
Če se v zbirnem vzorcu, vzetim petim prašičem, odkrije prisotnost trihinel, se od posameznih prašičev iz skupine odvzame nadaljnjih 20 g vzorcev, pri čemer se vsakega pregleda ločeno po v tem poglavju opisani metodi.
The procedure can be repeated 2 to 4 times until the fluid is clear enough for a reliable reading.
Vzorci parazitov se hranijo v 90 % etanolu, da se ohranijo in da lahko referenčni laboratorij EU ali nacionalni referenčni laboratorij identificira vrsto parazitov.
Pools of less than 100 g
Po odvzemu parazitov je treba pozitivne tekočine (digestivno tekočino, supernatant, izpiralno vodo itd.) razkužiti s segrevanjem na najmanj 60 °C.
Where needed, up to 15 g can be added to a total pool of 100 g and examined together with these samples in accordance with Section I. More than 15 g must be examined as a complete pool.
Postopek čiščenja in razkuževanja po pozitivnem ali nejasnem rezultatu
For pools of up to 50 g, the digestion fluid and the ingredients may be reduced to 1 litre of water, 8 ml of hydrochloric acid and 5 g of pepsin.
Kadar je pri pregledu zbirnega ali posameznega vzorca rezultat pozitiven ali nejasen, je treba ves material, ki je bil v stiku z mesom (posodo mešalca in rezilo, čašo, mešalno paličico, senzor temperature, stožčasti lijak za filtriranje, sito in klešče) temeljito razkužiti tako, da se ga umije v topli vodi (65 °C do 90 °C).
Positive or doubtful results
Priporoča se, da se vsak kos temeljito spere, da se odstrani detergent, če se uporablja med pranjem.
Where examination of a collective sample produces a positive or uncertain result, a further 20 g sample is taken from each pig in accordance with point 2(a).
ENAKOVREDNE METODE
The 20 g samples from five pigs are pooled and examined using the method described in this Chapter.
A. Mehanska metoda prebave zbirnega vzorca/sedimentacijska tehnika
In this way samples from 20 groups of five pigs will be examined.
nož ali škarje za rezanje vzorcev;
When Trichinella is detected in a pooled sample from five pigs, further 20 g samples are collected from the individual pigs in the group and each is examined separately using the method described in this Chapter.
pladnji, razdeljeni na 50 kvadratov, od katerih lahko vsak drži vzorec po približno 2 g mesa, ali druga orodja, ki zagotavljajo enaka jamstva v zvezi s sledljivostjo vzorcev;
Parasite samples are to be kept in 90 % ethyl alcohol for conservation and identification at species level at the EU or national reference laboratory.
Strojček za mletje mesa ali električni mešalec.
After parasite collection, positive fluids (digestive juice, supernatant fluid, washings, etc.) are to be decontaminated by heating to at least 60 °C.
Mešalec Stomacher Lab 3 500 Thermo model.
Cleaning and decontamination procedure after a positive or doubtful result
Plastične vrečke za mešalec Stomacher Lab.
When the examination of a collective or individual sample produces a positive or doubtful result, all material in contact with meat (blender bowl and blade, beaker, stirring rod, temperature sensor, conical filtration funnel, sieve and forceps) must be carefully decontaminated by washing in warm water (65 to 90 °C).
Konični ločilni lijaki s prostornino 2 litrov, po možnosti opremljeni s teflonskimi varnostnimi čepi.
It is recommended to rinse each piece thoroughly to remove the detergent if a detergent is used during washing.
Sita s 180-mikrometrsko mrežo iz nerjavečega jekla ali medenine in zunanjim premerom 11 cm.
EQUIVALENT METHODS
Stekleni merilni valji s prostornino 100 ml.
A. Mechanically assisted pooled sample digestion method/sedimentation technique
Vibrator, npr. električni brivnik brez glave.
Knife or scissors for cutting specimens.
Rele, ki se vklaplja in izklaplja v časovnih razmakih ene minute.
Trays marked off with 50 squares, each of which can hold samples of approximately 2 g of meat, or other tools giving equivalent guarantees as regards the traceability of the samples.
Trihinoskop s horizontalno ploščo ali stereomikroskop z nastavljivim svetlobnim virom.
Meat mincer or electrical blender.
Posoda za štetje ličink in več petrijevk s premerom 9 cm, kot je navedeno v poglavju I(1), točki (l) in (m).
A Stomacher lab-blender 3 500 thermo model.
17,5 % klorovodikova kislina.
Plastic bags suitable for the Stomacher lab-blender.
pepsin z močjo:
Conical separation funnels, capacity 2 litres, preferably fitted with Teflon safety plugs.
1:10000 NF (US National Formulary), kar ustreza 1:12500 BP (British Pharmacopoeia) in 2000 FIP (Fédération internationale de pharmacie), ali stabilizirana raztopina pepsina z najmanj 660 enot Evropske farmakopeje/ml;
Sieves, mesh size 180 microns, external diameter 11 cm, with stainless steel or brash mesh.
več 10-litrskih posod za razkužitev opreme, npr. s formalinom, in za preostalo digestivno tekočino, če so vzorci pozitivni;
100 ml glass measuring cylinders.
tehtnica z natančnostjo 0,1 g;
A vibrator, e.g. an electric shaver with the head removed.
Kot je določeno v Poglavju I(2).
A relay which will switch on and off at one-minute intervals.
I. Mletje
A trichinoscope with a horizontal table or a stereo-microscope, with a sub-stage transmitted light source of adjustable intensity.
Če se vzorci mesa predhodno zmeljejo v strojčku za mletje mesa, se izboljša kakovost prebave.
A larval counting basin and a number of 9 cm diameter petri dishes as in Chapter I(1), points (l) and (m).
Če se uporabi električni mešalec, ga je treba vključiti tri- do štirikrat, tako da vsakič deluje približno po eno sekundo.
17,5 % hydrochloric acid.
Postopek prebave
Pepsin, strength:
Postopek lahko vključuje popolne skupke (100 g vzorcev hkrati) ali skupke manj kot 100 g.
1:10000 NF (US National Formulary) corresponding to 1:12500 BP (British Pharmacopoeia) and to 2000 FIP (Fédération internationale de pharmacie), or stabilised liquid pepsin with minimum 660 European Pharmacopoeia units/ml.
Popolni skupki (100 vzorcev hkrati):
A number of 10 litre bins to be used for decontamination of apparatus, e.g. with formol, and for digestive juice remaining where specimens test positive.
Mešalec Stomacher Lab 3 500 je opremljen z dvojno plastično vrečko, temperaturno kazalo pa je nastavljeno na 40 do 41 °C.
A balance accurate to 0,1 g.
V notranjo plastično vrečko se vlije liter in pol vode, segrete na 40 do 41 °C.
As stipulated in Chapter I(2).
Vodi v mešalcu Stomacher se doda 25 ml 17,5 % klorovodikove kisline.
I. Grinding
Nato se doda 100 vzorcev po približno 1 g (pri 25 do 30 °C), ki so bili vzeti od posameznih vzorcev v skladu s točko 2.
Grinding the meat samples in a meat mincer beforehand will improve the digestion quality.
Nazadnje se doda 6 g pepsina ali 18 ml raztopina pepsina.
If an electrical blender is used, the blender must be operated three to four times for approximately one second each time.
Ta vrstni red je treba strogo upoštevati, da se prepreči razkroj pepsina.
Digestion procedure
Stomacher nato 25 minut melje vsebino vrečke.
This procedure may involve complete pools (100 g of samples at a time) or pools of less than 100 g.
Plastična vrečka se odstrani iz Stomacherja, digestivna tekočina pa se prefiltrira skozi sito v 3-litrsko čašo.
Complete pools (100 samples at a time):
Plastična vrečka se splakne s približno 100 ml vode, s katero se nato splakne sito in nazadnje doda filtratu v čašo.
The Stomacher lab-blender 3 500 is fitted with a double plastic bag and the temperature control set at 40 to 41 °C.
K skupku 100 vzorcev se lahko doda do 15 posameznih vzorcev, ki se pregledajo skupaj s temi vzorci.
One and a half litres of water preheated to 40 to 41 °C is poured into the inner plastic bag.
Manjši skupki (manj kot 100 vzorcev):
25 ml of 17,5 % hydrochloric acid is added to the water in the Stomacher.
Digestivno tekočino se pripravi tako, da se zmeša en liter in pol vode ter 25 ml 17,5 % klorovodikove kisline.
100 samples weighing approximately 1 g each (at 25 to 30 °C) taken from each individual sample in accordance with point 2 are added.
Doda se 6 g pepsina in vse skupaj zmeša pri temperaturi med 40 in 41 °C.
Lastly, 6 g pepsin or 18 ml liquid pepsin is added.
Od digestivne tekočine se odmeri količina, ki ustreza 15 ml na gram vzorca (npr. za 30 vzorcev je potrebna količina 30 × 15 ml = 450 ml), ki se doda v obe plastični vrečki skupaj z vzorci mesa po približno 1 g (pri 25 do 30 °C), vzetih od posameznih vzorcev v skladu s točko 2.
This order must be followed strictly to avoid decomposition of the pepsin.
Voda pri temperaturi približno 41 °C se vlije v zunanjo vrečko, dokler v obeh vrečkah skupna prostornina ne znaša en liter in pol.
The Stomacher is then allowed to pound the content of the bag for 25 minutes.
Plastična vrečka se splakne s približno 100 ml vode (pri 25 do 30 °C), s katero se nato splakne sito in nazadnje doda filtratu v čašo.
The plastic bag is removed from the Stomacher and the digestion fluid is filtered through the sieve into a 3 litre beaker.
Ugotavljanje prisotnosti ličink s sedimentacijo
The plastic bag is washed with approximately 100 ml of water, which is then used to rinse the sieve and lastly added to the filtrate in the beaker.
Digestivni tekočini se doda led (300 do 400 g ledenih ploščic, ledenega praška ali zdrobljenega ledu), ki njen volumen poveča na približno 2 litra.
Up to 15 individual samples can be added to a total pool of 100 samples and examined together with these samples.
Digestivna tekočina se nato meša, dokler se led ne raztopi.