natgillin/translations-raw huggingface dataset:
preparation of dietary, drinking water or inhalation formulations;
Elektroporacija
analytical determinations on formulations (e.g. stability, homogeneity, nominal concentrations);
uporaba električnih impulzov za povečanje prepustnosti celičnih membran.
species/strain used and justification for the choice;
Endogeni gen
number, age and sex of animals;
gen, ki izvira iz genoma.
source, housing conditions, diet, etc.;
Povečana binomska porazdelitev
individual weight of the animals at the start of the test, including body weight range, mean and standard deviation for each group;
večja variabilnost pri ponovitvi ocene deleža populacije, kot bi bila pričakovana, če bi imela populacija binomsko porazdelitev.
positive and negative (vehicle/solvent) control data;
Premik bralnega okvira
data from the range-finding study;
genetska mutacija, ki jo povzročijo insercije ali delecije števila nukleotidov, ki ni enakomerno deljivo s tri v zaporedju DNA, ki kodira za proteine/peptide.
details of test chemical preparation;
Insercija
rationale for route of administration;
v zaporedje DNA je dodan najmanj en nukleotidni bazni par.
methods for measurement of animal toxicity, including, where available, histopathological or haematological analyses and the frequency with which animal observations and body weights were taken;
veliko število mutantov, ki s klonalno ekspanzijo nastanejo iz ene same mutacije.
methods for verifying that the test chemical reached the target tissue, or general circulation, if negative results are obtained;
Obsežne delecije
actual dose (mg/kg body weight/day) calculated from diet/drinking water test chemical concentration (ppm) and consumption, if applicable;
delecije v DNA, ki obsegajo več kot nekaj kilobaz (učinkovito se jih odkriva v preskusih s selekcijo Spi– in plazmidom lacZ).
details of food and water quality;
Ligacija
detailed description of treatment and sampling schedules and justifications for the choices;
kovalentno povezovanje dveh koncev molekul DNA z ligazo DNA.
method of euthanasia;
kemikalija, ki celico spodbudi k celični delitvi, sproži mitozo (tj. celično delitev).
procedures for isolating and preserving tissues;
Nevtralni gen
methods for isolation of rodent genomic DNA, rescuing the transgene from genomic DNA, and transferring transgenic DNA to a bacterial host;
gen, na katerega ne vplivajo pozitivni ali negativni selektivni pritiski.
source and lot numbers of all cells, kits and reagents (where applicable);
Pakiranje
methods for enumeration of mutants;
sinteza infektivnih bakteriofagnih delcev od priprave kapside bakteriofaga in proteinov repa in konkatemer molekul DNA bakteriofaga.
methods for molecular analysis of mutants and use in correcting for clonality and/or calculating mutation frequencies, if applicable;
Običajno se uporablja za pakiranje DNA, klonirano na vektor lambda (ločeno s kohezivnimi konci) v infekciozne delce lambde.
Results:
Učinkovitost pakiranja
animal condition prior to and throughout the test period, including signs of toxicity;
učinkovitost, s katero so pakirani bakteriofagi integrirani v bakteriji gostiteljici.
body and organ weights at sacrifice;
Enote, ki delajo plake
for each tissue/animal, the number of mutants, number of plaques or colonies evaluated, mutant frequency;
merilo števila bakteriofagov, sposobnih za razmnoževanje.
for each tissue/animal group, number of packaging reactions per DNA sample, total number of mutants, mean mutant frequency, standard deviation;
Točkovna mutacija
dose-response relationship, where possible;
splošni izraz za mutacijo, ki vpliva le na majhno zaporedje DNA, vključno z majhnimi insercijami, delecijami in zamenjavami baznih parov.
for each tissue/animal, the number of independent mutants and mean mutation frequency, where molecular analysis of mutations was performed;
Pozitivna selekcija
concurrent and historical negative control data with ranges, means and standard deviations;
metoda, ki preživetje omogoči samo mutantom.
concurrent positive control (or non-concurrent DNA positive control) data;
Poročevalski gen
analytical determinations, if available (e.g. DNA concentrations used in packaging, DNA sequencing data);
gen, pri katerem se hitro odkrije mutirani genski produkt.
statistical analyses and methods applied;
Vzorčenje
Conclusion
konec obdobja, pred usmrtitvijo, med katerim žival ne prejema kemikalije in med katerim se nepopravljene poškodbe DNA fiksirajo v stabilne mutacije.
Skopek (1987), “Statistical Test for the Comparison of Samples from Mutational Spectra”, J. Mol.
Vektor za prenos
, “A more Efficient Big Blue® Protocol Improves Transgene Rescue and Accuracy in an Adduct and Mutation Measurement”, Mutation Res., 518:
vektor, ki je zgrajen tako, da se lahko razširi v dveh različnih vrstah gostiteljev;
Gossen and J. Vijg (1995), “Plasmid-based Transgenic Mouse Model for Studying in vivo Mutations”Nature, 377(6550):
zato je mogoče DNA, vstavljeno v prenosljivi vektor, preskušati ali jo obravnavati v dveh različnih vrstah celic ali dveh različnih organizmih.
Gorelick (1995), “Statistical Design and Analysis of Mutation Studies in Transgenic Mice”, Environ.
Transgensko
Soper(1995), “Temporal and Molecular Characteristics of Mutations Induced by Ethylnitrosourea in Germ Cells Isolated from Seminiferous Tubules and in Spermatozoa of lacZ Transgenic Mice”, Proc.
V tej tabeli je predstavljeno najmanjše število meritev, ki jih je treba izvesti.
Soper and J. Jiao (1997), “Toward an Understanding of the Use of Transgenic Mice for the Detection of Gene Mutations in Germ Cells”, Mutation Res., 388(2-3):
Glede na pričakovane učinke in rezultate začetnih meritev se lahko priporoča dodajanje dodatnih časovnih točk (npr. stare živali) ali opravljanje meritev v drugih razvojnih fazah.
Tindall (2000), “Bayesian Analysis of Mutational Spectra”, Genetics, 156:
Priporoča se, da se preskusi na mladičih ne izvajajo dva dni po odstavitvi od sesanja (glej odstavek 32).
Knook and J. Vijg(1989), “Efficient Rescue of Integrated Shuttle Vectors from Transgenic Mice: a Model for Studying Mutations in vivo”, Proc.
Priporočeni starosti za preskuse v adolescenci sta:
Gossen, J.A. and J. Vijg (1993), “A Selective System for lacZ-Phage using a Galactose-sensitive E. coli Host”, Biotechniques, 14(3):
učenje in spominjanje = PPD 25 ± 2;
, “Somatic Gene Mutation and Human Disease other than Cancer”, Mutation Res., 543:
motorična in senzorična funkcija = PPD 25 ± 2.
, “Somatic Gene Mutation and Human Disease other than Cancer: an Update”, Mutation Res., 705:
Priporočena starost za preskuse na mladih odraslih je PPD 60–70.
Douglas and D. Krewski (1998), “Statistical Analysis of lacZ Mutant Frequency Data from Muta™Mouse Mutagenicity Assays”, Mutagenesis, 13(3):
Telesno težo je treba v primeru neposrednega odmerjanja izmeriti vsaj dvakrat na teden, da se odmerki prilagodijo v času hitrega pridobivanja telesne teže.
Douglas (2003), “Treatment and Sampling Protocols for Transgenic Mutation Assays”, Environ.
Druge razvojne znake, poleg telesne teže (npr. odpiranje oči), je treba zabeležiti, kadar je primerno (glej odstavek 31).
Muller, B. Paul, S. Adhya and S. Garges (1996), “Analysis of Genetic Instability during Mammary Tumor Progression using a novel Selection-based Assay for in vivo Mutations in a Bacteriophage λ Transgene Target”, Proc.
Glej odstavek 35.
Short (1990), “The Use of Transgenic Mice for Short-term, in vivo Mutagenicity Testing”, Genet.
Mejni koeficient variacij za zadevno tkivo je bil določen s pomočjo grafa vrednosti koeficienta variacij – zaporedno podanih od najnižje do najvišje – za vse srednje vrednosti iz vseh preskusov v validaciji z določenim modelom (z agonistom ali antagonistom).
Burns (2008), “Comparison of Induced and Cancer-associated Mutational Spectra using Multivariate Data Analysis”, Carcinogenesis, 29(4):
Mejna vrednost koeficienta variacij je bila odčitana s točke, pri kateri so vmesna povečanja do naslednjega najvišjega koeficienta variacij v seriji precej večja kot prejšnjih nekaj koeficientov variacij – 'točka preloma'.
Nohmi, T., M. Katoh, H. Suzuki, M. Matsui, M. Yamada, M. Watanabe, M. Suzuki, N. Horiya, O. Ueda, T. Shibuya, H. Ikeda and T. Sofuni (1996), “A new Transgenic Mouse Mutagenesis Test System using Spi– and 6-thioguanine Selections”, Environ.
Opozoriti je treba, da so bile s to analizo sicer opredeljene relativno zanesljive 'točke preloma' za preskus z antagonistom, vendar so krivulje koeficienta variacij za preskus z agonistom pokazale bolj enakomerno povečanje, zaradi česar je opredelitev mejne vrednosti koeficienta variacij s to metodo do neke mere arbitrarna.
Nohmi, T., M. Suzuki, K. Masumura, M. Yamada, K. Matsui, O. Ueda, H. Suzuki, M. Katoh, H. Ikeda and T. Sofuni (1999), “Spi– Selection: an Efficient Method to Detect γ-ray-induced Deletions in Transgenic Mice”, Environ.
Raziskave so pokazale, da je mlečna žleza, zlasti v zgodnji fazi svojega razvoja, občutljiva končna točka za estrogensko delovanje.
Masumura (2000), “Recent Advances in the Protocols of Transgenic Mouse Mutation Assays”, Mutation Res., 455(1–2):
Priporoča se, da se pri validaciji v to metodo vključijo končne točke v zvezi z mlečnimi žlezami obeh spolov.
OECD (2000), Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpoints for Experimental Animals Used in Safety Evaluation, Series on Testing and Assessment, No 19, ENV/JM/MONO(2000)7, OECD, Paris.
‚Nova serija‘ pomeni svežo serijo celic, prejetih od dobavitelja ATCC.
OECD (2009), Detailed Review Paper on Transgenic Rodent Mutation Assays, Series on Testing and Assessment, No 103, ENV/JM/MONO(2009)7, OECD, Paris.
‚Zamrznjena serija‘ pomeni celice, ki so že bile gojene in nato zamrznjene v laboratoriju, ki ni laboratorij dobavitelja ATCC.
Tindall (1995), “Study Design and Sample Sizes for a lacI Transgenic Mouse Mutation Assay”, Environ.
Če je potrebna ekstrakcija, se za posamezen ekstrakt opravijo tri ponovne meritve.
Gorelick (1997), “Sources of Variability in Data from a Positive Selection lacZ Transgenic Mouse Mutation Assay: an Interlaboratory Study”, Mutation.
Vsak vzorec se ekstrahira samo enkrat.
Yauk (2006), “Detection of Induced Male Germline Mutation: Correlations and Comparisons between Traditional Germline Mutation Assays, Transgenic Rodent Assays and Expanded Simple Tandem Repeat Instability Assays”, Mutation.
Meje meritve metode temeljijo na vrednostih osnovne proizvodnje hormonov iz tabele 5 in učinkovitosti.
Kurata, M. Takamune, M. Yamada, H. Sanada, T. Umemura, A. Nishikawa and T. Nohmi (2010), “Integration of in vivo Genotoxicity and Short-term Carcinogenicity Assays using F344 gpt delta Transgenic Rats: in vivo Mutagenicity of 2,4-diaminotoluene and 2,6-diaminotoluene Structural Isomers”, Toxicol.
Če se lahko doseže večje osnovno proizvajanje hormonov, je lahko meja višja.
Martus, J.C. Mirsalis, T. Suzuki and N. Yajima (2003), “In vivo Transgenic Mutation Assays”, Mutation Res., 540:
Nekatera protitelesa za T in E2 so lahko pri večjih deležih navzkrižno reaktivna z androstendionom in estronom.
Douglas (1996), “Bacteriophage λ and Plasmid lacZ Transgenic Mice for studying Mutations in vivo” in:
V takih primerih ni mogoče točno določiti učinkov na 17β-HSD.
Douglas (2005), “A lacZ Transgenic Mouse Assay for the Detection of Mutations in Follicular Granulosa Cells”, Mutation Res., 578(1-2):
Vendar lahko podatki kljub temu zagotavljajo uporabne informacije o učinkih na proizvajanje estrogena ali androgena na splošno.
Martus, V. Thybaud (2011), “Compilation and Use of Genetic Toxicity Historical Control Data”, Mutation Res., doi:10.1016/j.mrgentox.2010.09.007.
V takih primerih je treba podatke izraziti kot odzive androgena/estrogena in ne kot E2 in T.
OECD (2011), Retrospective Performance Assessment of OECD Test Guideline on Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays, Series on Testing and Assessment, No 145, ENV/JM/MONO(2011)20, OECD, Paris.
Ti vključujejo:
, “Kinetics of spermatogenesis in mammals seminiferous epithelium cycle and spermatogonial renewal”.
holesterol, pregnenolon, progesteron, 11-deoksikortikosteron, kortikosteron, aldosteron, 17α-pregnenolon,17α-progesteron, deoksikortizol, kortizol, DHEA, androstendion, estron.
, “The Epididymis”, in Neil, J.D., Pfaff, D.W., Chalis, J.R.G., de Kretser, D.M., Richards, J.S., and P. M, Wassarman (eds.), Physiology of Reproduction, Elsevier, the Netherlands, pp. 1071-1148.
Kontrolni vzorec (0) s topilom (DMSO), 1 μl DMSO/jamico.
, “Effects of male germ-cell stage on the frequency, nature, and spectrum of induced specific-locus mutations in the mouse”, Genetica, 122:
+ : pozitivno.
Administration period
Celice v praznih jamicah prejmejo le gojišče (tj. nič topila).
the total period during which an animal is dosed.
Metanol (MeOH) se doda po koncu izpostavljenosti in ko je gojišče odstranjeno iz teh jamic.
Base pair substitution
Kontrolni vzorec s topilom DMSO (1 μl/jamico).
a type of mutation that causes the replacement of a single DNA nucleotide base with another DNA nucleotide base.
Potrditev prejšnjega poteka preskusa z uporabo enake zasnove preskusa.
Capsid
Ponovitev preskusa pri razporeditvi koncentracij z 1/2-logaritmom (omejitev koncentracij, ki so se v predhodnem preskusu izkazale za znatno različne).
the protein shell that surrounds a virus particle.
Razmerje spremembe pri eni koncentraciji se statistično znatno razlikuje od kontrolnega vzorca z raztopino.
a substance or a mixture.
Nanaša se na ponovljene meritve istega vzorca.
Clonal expansion
Študija razvojne nevrotoksičnosti se lahko izvede kot ločena študija, lahko je vključena v študijo strupenosti za razmnoževanje in/ali nevrotoksičnosti pri odraslih (npr. preskusne metode B.34 (14), B.35 (15) in B.43 (16)) ali dodana študiji strupenosti za predrojstveni razvoj (npr. preskusna metoda B.31 (17)).
the production of many cells from a single (mutant) cell.
Če je študija razvojne nevrotoksičnosti vključena v drugo študijo ali ji je dodana, je treba ohraniti celovitost obeh vrst študij.
a measure of viable bacterial numbers.
Preskuševalni laboratorij mora pred začetkom izvajanja študije preučiti vse razpoložljive informacije o preskusni kemikaliji.
Concatamer
Takšne informacije vključujejo vrsto in strukturo kemikalije, njene fizikalno-kemijske lastnosti, rezultate katerih koli drugih preskusov strupenosti kemikalije in vitro ali in vivo, toksikološke podatke o strukturno sorodnih kemikalijah in pričakovane uporabe kemikalije.
a long continuous biomolecule composed of multiple identical copies linked in series.
Te informacije so potrebne, da se vsem, ki jih to zadeva, zagotovi, da je preskus pomemben za zaščito zdravja ljudi in da bo v pomoč pri izbiri ustreznega začetnega odmerka.
Cos site
učinke na absorpcijo, distribucijo, presnovo ali retencijo preskusne kemikalije, učinke na kemijske lastnosti preskusne kemikalije, ki lahko spremenijo njene strupene lastnosti, ter učinke na porabo hrane ali vode ali status prehranjenosti živali.
a 12-nucleotide segment of single-stranded DNA that exists at both ends of the bacteriophage lambda's double-stranded genome.
dodatna navodila so navedena v Smernici št. 43 (8).
a mutation in which one or more (sequential) nucleotides is lost by the genome.
Pri potomcih, usmrčenih na PPD 22, je treba pregledati možganska tkiva, pri živalih, usmrčenih ob koncu, pa je treba pregledati tkiva centralnega živčnega sistema in perifernega živčnega sistema.
Electroporation
Copenhagen, Denmark, 13–14 June 1995.
the application of electric pulses to increase the permeability of cell membranes.
Na voljo na:
Endogenous gene
Health Perspect., 109:79–91.
a gene native to the genome.
Health Perspect., 109:101–111.
Extrabinomial variation
Health Perspect., 109:93–100.
greater variability in repeat estimates of a population proportion than would be expected if the population had a binomial distribution.
Washington D.C., US, 23–25 October 2000.
Frameshift mutation
July 2008.
a genetic mutation caused by insertions or deletions of a number of nucleotides that is not evenly divisible by three within a DNA sequence that codes for a protein/peptide.
Na voljo na:
Insertion
Experimental and Clinical Neurotoxicology, 2nd Edition, ISBN 0195084772, Oxford University Press, New York.
the addition of one or more nucleotide base pairs into a DNA sequence.
Handbook of Developmental Neurotoxicology, 1stEdition, ISBN 0126488606, Academic Press, New York.
Jackpot
WHO (1986) Principles and Methods for the Assessment of Neurotoxicity Associated with Exposure to Chemicals (Environmental Health Criteria 60), Albany, New York:
a large number of mutants that arose through clonal expansion from a single mutation.
Approaches and Methods, 1stEdition, ISBN 012168055X, Academic Press, New York.
Large deletions
Teratol., 19:499–509.
deletions in DNA of more than several kilobases (which are effectively detected with the Spi- selection and the lacZ plasmid assays).
Toxicol., 38:2–6.
Ligation
Animal Sci., 27:110–112.
the covalent linking of two ends of DNA molecules using DNA ligase.
Toxicol., 13:118–136.
Mitogen
Int.
a chemical that stimulates a cell to commence cell division, triggering mitosis (i.e. cell division).
J. Toxicol., 24:87–94.
Neutral gene
Toxicol., 6:433–439.
a gene that is not affected by positive or negative selective pressures.
Teratol., 20:449–457.
Packaging
Toxicol., 13:383–390.
the synthesis of infective phage particles from a preparation of phage capsid and tail proteins and a concatamer of phage DNA molecules.
Sci., 58:350–365.
Commonly used to package DNA cloned onto a lambda vector (separated by cos sites) into infectious lambda particles.
Teratol., 7:579–586.
Packaging efficiency
Reprod., 17:298–303.
the efficiency with which packaged bacteriophages are recovered in host bacteria.
Teratol., 12:489–95.
Plaque forming unit (pfu)
Behav., 23:896–920.
a measure of viable bacteriophage numbers.
Toxicol., 1:53–66.
Point mutation
A critique of the methods available for the measurement of spontaneous motor activity, Handbook of Psychopharmacology, Vol.
a general term for a mutation affecting only a small sequence of DNA including small insertions, deletions, and base pair substitutions.
7, Iverson, L.L., Iverson, D.S., Snyder, S.H., (eds.)
Positive selection
Plenum Press, New York, str. 37–82.
a method that permits only mutants to survive.
Teratol., 15:117–129.
Reporter gene
Psychobiol., 18:247–260.
a gene whose mutant gene product is easily detected.
Teratol., 13:599–609.
Sampling time
405–411.
the end of the period of time, prior to sacrifice, during which the chemical is not administered and during which unprocessed DNA lesions are fixed into stable mutations.
A force plate system for measuring low-magnitude reaction forces in small laboratory animals.
Shuttle vector
Physiol.
a vector constructed so that it can propagate in two different host species;
Behav., 64:661–669.
accordingly, DNA inserted into a shuttle vector can be tested or manipulated in two different cell types or two different organisms.
Pharmacol., 40:589–591.
Transgenic
Neurobiol., 59:107–128.
of, relating to, or being an organism whose genome has been altered by the transfer of a gene or genes from another species.’
Toxicol., 8:199–211.
Depending on the sensitivity of cognitive function tests, investigation of a large higher number of animals should be considered e.g. up to 1 male and 1 female per litter (for animal assignments see Appendix 1) (further guidance on sample size is provided in the OECD Guidance Document 43 (8)).
Res., 80:25–30.
This table presents the minimum number of times when measurements should be performed.
Pharmacol., 105:321–332.
Depending on the anticipated effects, and the results of the initial measurements, it may be advisable to add additional time points (e.g. aged animals) or to perform the measurements in other developmental stages.
Rev., 6:247–296.
It is recommended that pups not be tested during the two days after weaning (see paragraph 32).
Teratol., 10:237–244.
Recommended ages for adolescent testing are:
Neurosci., 103:98–105.
learning and memory = PND 25 ± 2;
Psychobiol., 17:465–479.
motor and sensory function = PND 25 ± 2.
Methods, 11:47–60.
Recommended ages for testing young adults is PND 60-70.
Int.
Body weights should be measured at least twice weekly when directly dosing pups for adjustment of doses at a time of rapid body weight gain.
J. Neurosci., 48:29–69.
Brain weights and neuropathology may be assessed at some earlier time (e.g. PND 11), if appropriate (see paragraph 39).
Teratol., 9:235–241.
Other developmental landmarks in addition to the body weight (e.g. eye opening) should be recorded when appropriate (see paragraph 31).
Toxicol., 20:387–399.
See paragraph 35.
Teratol., 10:327–332.
The threshold CV for a given tissue was identified from a graph of CV values — arranged from smallest sequentially to largest — for all means from all experiments in the validation exercise using a specific model (agonist or antagonist).
Pharmacol., 71:342–352.
The threshold CV was read from the point at which the increments between to the next highest CVs in the series are dramatically larger than the preceding few CVs- the “breakpoint”.
Laboratory Methods in Histotechnology, American Registry of Pathology, Washington, DC, str. 84–107.
It should be noted that although this analysis identified relatively reliable “breakpoints” for the antagonist model of the assay, CV curves for the agonist assay showed a more uniform increase making identification of a threshold CV by this method somewhat arbitrary.
Brain Res., 874:123–130.
Research has shown the mammary gland, especially in early life mammary gland development, to be a sensitive endpoint for oestrogen action.
Pathol., 53:365–372.
It is recommended that endpoints involving pup mammary glands of both sexes be included in this test method, when validated.
Teratol., 16, 545–561.
“New batch” refers to a fresh batch of cells received from ATCC.
Pharmacol., 19:745–755.
“Frozen batch” refers to cells that have been previously cultured and then frozen at a laboratory other than ATCC.
Toxicol., 20:417–432.
If extraction is required, three replicate measurements are made for each extract.
J. Neurocytol., 26:707–710.
Method measurement limits are based on the basal hormone production values provided in Table 5, and are performance based.
Teratology, 49:113–121.
If greater basal hormone production can be achieved the limit can be greater.
Brain Res., 84:294–298.
Some T and E2 antibodies may cross-react with androstendione and oestrone, respectively, at a greater percentage.
Science, 283:70–74.
In such cases it is not possible to accurately determine effects on 17β-HSD.
Let., 63:127–133.
However, the data can still provide useful information regarding the effects on oestrogen or androgen production in general.
Health Perspect., 104:401–405.
In such cases data should be expressed as androgen/oestrogen responses rather than E2 and T.
56274–56322.
These include:
Neurotoxicology, 5:113–126.
cholesterol, pregnenolone, progesterone, 11-deoxycorticosterone, corticosterone, aldosterone, 17α-pregnenolone,17α-progesterone, deoxycortisol, cortisol, DHEA, androstenedione, oestrone.
Toxicol., 8:21–27.
Cells in Blank wells receive medium only (i.e. no solvent).
Teratol., 7:587–90.
DMSO solvent control (1 μl/well).
34:296–313.
Re-run assay at 1/2-log concentration spacing (bracketing the concentration that tested significantly different in the preceding experiment).
Biometrics, 41:295–301.
Refers to replicate measures of the same sample
Neurotoxicology and Teratology, 30(4):288–325.
A developmental neurotoxicity study can be conducted as a separate study, incorporated into a reproductive toxicity and/or adult neurotoxicity study (e.g. test methods B.34 (14), B.35 (15), B.43 (16)), or added onto a prenatal developmental toxicity study (e.g. test method B.31 (17)).
a report from the ILSI Research Foundation/Risk Science Institute expert working group on neurodevelopmental endpoints Neurotoxicology and Teratology, 30(4):349–381.
All testing should comply with applicable legislation or government and institutional guidelines for the use of laboratory animals in research (e.g. 18).
Če študija osnovne pozitivne kontrole ne prinese pričakovanih rezultatov, je treba pogoje preskusa preveriti in spremeniti.