natgillin/translations-raw huggingface dataset:
Reference solution for thin-layer chromatography: cholesterol or phytosterols, and erythrodiol 5 % solution in ethyl acetate (point 4.11).
Referenčna raztopina za tankoplastno kromatografijo: holesterol ali fitosteroli in 5-odstotna raztopina eritrodiola v etilacetatu (točka 4.11).
2,7-dichlorofluorescein, 0,2 % in ethanolic solution.
2,7-diklorofluorescein, 0,2-odstotna etanolna raztopina.
Make slightly basic by adding a few drops of 2 N alcoholic potassium hydroxide solution (point 4.2).
Naredite rahlo bazično z dodatkom nekaj kapelj alkoholne raztopine 2 N kalijevega hidroksida (točka 4.2).
Anhydrous pyridine, for chromatography quality (see Note 5).
Brezvodni piridin kromatografske čistoče (glej opombo 5).
Hexamethyl disilazane of analytical quality.
Heksametil disilazan čistoče za analizo.
Trimethylchlorosilane of analytical quality.
Trimetilklorosilan čistoče za analizo.
Sample solutions of sterol trimethylsilyl ethers.
Vzročne raztopine sterolnih trimetilsililnih etrov.
To be prepared at the time of use from sterols and erythrodiol obtained from oils containing them.
Pripravite jih tik pred uporabo iz sterolov in eritrodiola iz olj, ki jih vsebujejo.
α-cholestanol, purity more than 99 % (purity must be checked by GC analysis).
α-holestanol več kot 99-odstotne čistoče (čistost je treba preveriti s plinskokromatografsko analizo).
α-cholestanol internal standard solution, 0,2 % solution (m/V) in ethyl acetate (point 4.11).
0,2-odstotna interna standardna raztopina α-holestanola (m/V) v etilacetatu (točka 4.11).
Phenolphthalein solution, 10 g/L in ethanol (point 4.10).
Raztopina 10 g/l fenolftaleina v etanolu (točka 4.10).
Carrier gas: hydrogen or helium, gas-chromatographic purity.
Nosilni plin: vodik ali helij plinskokromatografske čistoče.
Auxiliary gases: hydrogen, helium, nitrogen and air, of gas-chromatographic purity.
Pomožni plini: vodik, helij, dušik in zrak plinskokromatografske čistoče.
n-Hexane (point 4.9)/ethyl ether (point 4.10) mixture 65:35 (V/V).
Mešanica n-heksana (točka 4.9) in etilnega etra (točka 4.10) v razmerju 65: 35 (V/V).
Silylation reagent, consisting of a 9:3:1 (V/V/V) mixture of pyridine/hexamethyl disilazane/trimethylchlorosilane.
Reagent za sililiranje, sestavljen iz mešanice piridina, heksametildisilizana in trimetilklorosilana v razmerju 9: 3: 1 (V/V/V).
Preparation of the unsaponifiable matter.
Priprava neumiljivih snovi
Using a 500 μl microsyringe (point 3.6) introduce into the 250 ml flask (point 3.1) a volume of the α-cholestanol internal standard solution (point 4.20) containing an amount of cholestanol corresponding to approximately 10 % of the sterol content of the sample.
S 500 μl mikrobrizgalko (točka 3.6) vnesite v 250 ml bučko (točka 3.1) interno standardno raztopino α-holestanola (točka 4.20), ki vsebuje količino holestanola, ki ustreza približno 10 % vsebnosti sterolov v vzorcu.
For example, for 5 g of olive oil sample add 500 μl of the α-cholestanol solution (point 4.20) and 1500 μl for olive-pomace oil.
Na primer za 5 g vzorca oljčnega olja dodajte 500 μl raztopine α-holestanola (točka 4.20), za olje iz oljčnih tropin pa 1500 μl.
Evaporate until dryness with a gentle current of nitrogen in a warm water bath, after cooling the flask, weigh 5 ± 0,01 g of the dry filtered sample into the same flask.
Po ohladitvi bučke raztopino odparite do suhega z blagim tokom dušika na parni kopeli, nato pa v isto bučko natehtajte 5 ± 0,01 g suhega filtriranega vzorca.
Animal or vegetable oils and fats containing appreciable quantities of cholesterol may show a peak having a retention time close to cholestanol.
Olja in masti živalskega ali rastlinskega izvora, ki vsebujejo precejšnje količine holesterola, lahko dajo vrh, ki ima retenzijski čas podoben holestanolu.
If this occurs, the sterol fraction will have to be analysed in duplicate with and without internal standard.
V takem primeru je treba sterolno frakcijo analizirati dvakrat, in sicer z internim standardom in brez njega.
Add 50 ml of 2 N ethanolic potassium hydroxide solution (point 4.2) and some pumice, fit the reflux condenser and heat to gentle boiling until saponification takes place (the solution becomes clear).
Dodajte 50 ml 2 N etanolne raztopine kalijevega hidroksida (točka 4.2) in nekaj plovca, namestite povratni hladilnik in segrejte na rahlo vretje do končanega umiljenja (raztopina postane bistra).
Continue heating for a further 20 minutes, then add 50 ml of distilled water from the top of the condenser, detach the condenser and cool the flask to approximately 30 °C.
S segrevanjem nadaljujte še nadaljnjih 20 minut in potem z vrha hladilnika dodajte 50 ml destilirane vode, snemite hladilnik in ohladite bučko na približno 30 °C.
Transfer the contents of the flask quantitatively into a 500 ml separating funnel (point 3.2) using several portions of distilled water (50 ml).
Kvantitativno prenesite vsebino bučke v 500 ml lij ločnik (točka 3.2), in sicer z večkratnim spiranjem z destilirano vodo (50 ml).
Add approximately 80 ml of ethyl ether (point 4.10), shake vigorously for approximately 60 seconds, periodically releasing the pressure by inverting the separating funnel and opening the stopcock.
Dodajte približno 80 ml etilnega etra (točka 4.10), močno tresite približno 60 sekund ter postopoma sproščajte pritisk, tako da obrnete lij ločnik in odprete petelinčka.
Allow standing until there is complete separation of the two phases (Note 2).
Pustite mirovati, dokler fazi nista popolnoma ločeni (opomba 2).
Then draw off the soap solution as completely as possible into a second separating funnel.
Nato milnico v celoti prelijte v drug lij ločnik.
Perform two further extractions on the water-alcohol phase in the same way using 60 to 70 ml of ethyl ether (point 4.10).
Iz vodno-alkoholne faze še dvakrat ekstrahirajte, in sicer z uporabo 60 do 70 ml etilnega etra (točka 4.10).
Note 2: Any emulsion can be destroyed by adding small quantities of ethanol (point 4.11).
Opomba 2: Emulzijo je mogoče odstraniti z dodajanjem majhnih količin etanola (točka 4.11).
Combine the three ether extracts in one separating funnel containing 50 ml of water.
Združite etrske ekstrakte v skupnem liju ločniku, ki vsebuje 50 ml vode.
Continue to wash with water (50 ml) until the wash water no longer gives a pink colour on the addition of a drop of phenolphthalein solution (point 4.21).
Nadaljujte z izpiranjem (50 ml), dokler izpiralna voda ob dodatku kapljice raztopine fenolftaleina ni več rožnato obarvana (točka 4.21).
When the wash water has been removed, filter on anhydrous sodium sulphate (point 4.5) into a previously weighed 250 ml flask, washing the funnel and filter with small quantities of ethyl ether (point 4.10).
Potem ko je izpiralna voda odstranjena, filtrirajte skozi brezvodni natrijev sulfat (točka 4.5) v predhodno stehtani 250 ml bučki, pri tem pa lij in filter izperite z majhnimi količinami etilnega etra (točka 4.10).
Evaporate the solvent by distillation on a rotary evaporator at 30 °C under vacuum.
Topilo odparite z destilacijo na rotacijskem izparilniku pri 30 °C v vakuumu.
Add 5 ml of acetone and remove the volatile solvent completely in a gentle current of air.
Dodajte 5 ml acetona in z blagim tokom zraka v celoti odstranite hlapno topilo.
Dry the residue in the oven at 103±2 °C for 15 min. Cool in desiccators and weigh to the nearest 0,1 mg.
Ostanek sušite v peči 15 minut pri 103 ± 2 °C. Po ohladitvi v eksikatorju ga stehtajte z natančnostjo 0,1 mg.
Separation of the sterol and triterpene dialcohols fraction (erythrodiol + uvaol)
Ločevanje sterolne in triterpenske dialkoholne frakcije (eritrodiol + uvaol)
Remove from the oven and keep in a calcium chloride desiccator (point 3.13) until required for use (plates treated in this way must be used within 15 days).
Vzemite jih iz peči in jih do uporabe hranite v eksikatorju, napolnjenem s kalcijevim kloridom (točka 3.13) (na ta način obdelane plošče je treba uporabiti v 15 dneh).
When basic silica gel plates are used to separate the sterol fraction there is no need to treat the unsaponifiable fraction with alumina.
Kadar se za ločevanje sterolnih frakcij uporabljajo z lugom obdelane silikagelne plošče, neumiljivih frakcij ni treba tretirati z aluminijevim oksidom.
In this way all compounds of an acid nature (fatty acids and others) are retained on the spotting line and the sterols band is clearly separated from the aliphatic and triterpene alcohols band.
Na ta način se vse sestavine, ki so kisle narave (maščobne kisline in druge), obdržijo na mestu napršitve, pas sterolov pa se jasno loči od pasov alifatskih in triterpenskih alkoholov.
Place hexane/ethyl ether mixture (point 4.24) (Note 4) into the development chamber, to a depth of approximately 1 cm.
Mešanico heksana in etilnega etra (točka 4.24) (opomba 4) vstavite v razvijalno komoro na globino približno 1 cm.
Close the chamber with the appropriate cover and leave thus for at least half an hour, in a cool place, so that liquid-vapour equilibrium is established strips of filter paper dipping into the eluent may be placed on the internal surfaces of the chamber.
Komoro zaprite z ustreznim pokrovom in jo tako pustite najmanj pol ure na hladnem, da se lahko med hlapi in tekočino ustvari ravnotežje. Trakovi filtrirnega papirja, namočeni v eluent, se lahko položijo na notranje površine komore.
This reduces developing time by approximately one-third and brings about more uniform and regular elution of the components.
To skrajša razvijalni čas za približno eno tretjino ter omogoča enotnejšo in bolj homogeno elucijo sestavin.
Note 4:
Opomba 4: Razvijalno mešanico je treba zamenjati za vsak preskus, zato da se dosežejo popolnoma ponovljivi pogoji elucije.
The developing mixture needs to be replaced for every test, in order to achieve perfectly reproducible elution conditions, alternative solvent 50:50 (V/V) n-hexane/ethyl ether may be used.
Kot alternativa se lahko uporabi mešanica n-heksana in etilnega etra v razmerju 50: 50 (V/V).
Prepare an approximately 5 % solution of the unsaponifiable (point 5.1.5) in ethyl acetate (point 4.12) and, using the 100 μl microsyringe, depose 0,3 ml of the solution on a narrow and uniform streak on the lower end (2 cm) of the chromatographic plate (point 5.2.1).
Pripravite približno 5-odstotno raztopino neumiljivih snovi (točka 5.1.5) v etilacetatu (točka 4.12) ter s pomočjo 100 μl mikrobrizgalke nanesite 0,3 ml raztopine na tanko in homogeno progo na nižjem koncu (2 cm) kromatografske plošče (točka 5.2.1).
In line with the streak, place 2 to 3 μl of the material reference solution (point 4.13), so that the sterol and triterpene dialcohols band can be identified after developing.
Vzporedno s progo nanesite 2 do 3 μl materialne referenčne raztopine (točka 4.13), tako da se po razvijanju sterolna in triterpenska dialkoholna pasova lahko identificirata.
Place the plate in the developing chamber prepared as specified in point 5.2.2.
Ploščo vstavite v razvijalno komoro, pripravljeno, kot je navedeno v točki 5.2.2.
The ambient temperature should be maintained between 15 and 20 °C (Note 5).
Temperaturo okolja je treba vzdrževati med 15 in 20 °C (opomba 5).
Immediately close the chamber with the cover and allow eluting until the solvent front reaches approximately 1 cm from the upper edge of the plate.
Komoro takoj zaprite s pokrovom in pustite, da poteka eluiranje, dokler fronta topila ne doseže razdalje 1 cm od vrhnjega roba plošče.
Remove the plate from the developing chamber and evaporate the solvent in a flow of hot air or by leaving the plate for a short while, under a hood.
Vzemite ploščo iz razvijalne komore in odparite topilo s tokom vročega zraka ali tako, da ploščo nekaj časa pustite v digestoriju.
Note 5: Higher temperature could worsen the separation.
Opomba 5: Višja temperatura lahko negativno vpliva na ločevanje.
Spray the plate lightly and uniformly with the 2,7-dichlorofluorescein solution (point 4.14) and then leave to dry.
Rahlo in enakomerno napršite ploščo z raztopino 2,7-diklorofluorosceina (točka 4.14) ter pustite, da se posuši.
When the plate is observed under ultraviolet light, the sterols and triterpene dialcohols bands can be identified through being aligned with the spots obtained from the reference solution (point 4.13).
Če se plošča opazuje pod ultravijolično svetlobo, se sterolna in triterpenska dialkoholna pasova lahko identificirata tako, da se poravnata s pikami, dobljenimi iz referenčne raztopine (točka 4.13).
Mark the limits of the bands along the edges of the fluorescence with a black pencil (see TLC plate figure 3).
S črnim svinčnikom označite meje pasov vzdolž roba fluorescence (glej tankoplastno kromatografijo na sliki 3).
By using a metal spatula, scrape off the silica gel of the marked area.
S kovinsko žličko odstrgajte silikagel na označeni površini.
Place the finely comminuted material removed into the filter funnel (point 3.7).
Odstranjen, v fin prah zdrobljen material dajte v filtrirni lij (točka 3.7).
Add 10 ml of hot ethyl acetate (point 4.12), mix carefully with the metal spatula and filter under vacuum, collecting the filtrate in the conical flask (point 3.8.) attached to the filter funnel.
Dodajte 10 ml vročega etilacetata (točka 4.12), s kovinsko žličko previdno zmešajte in v vakuumu filtrirajte, tako da filtrat zberete v erlenmajerici (točka 3.8), ki je pritrjena na filtrirni lij.
Wash the residue in the flask three times with ethyl ether (point 4.3) (approximately 10 ml each time), collecting the filtrate in the same flask attached to the funnel, evaporate the filtrate to a volume of 4 to 5 ml, transfer the residual solution to the previously weighed 10 ml test tube (point 3.9), evaporate to dryness by mild heating, in a gentle flow of nitrogen, make up again using a few drops of acetone (point 4.8), evaporate again to dryness,
Ostanek v erlenmajerici trikrat izperite z etilnim etrom (točka 4.3) (vsakokrat približno z 10 ml), tako da zberete filtrat v isti erlenmajerici, ki je pritrjena na lij. Odparite filtrat do prostornine 4 do 5 ml, prenesite preostanek raztopine v predhodno stehtano 10 ml epruveto (točka 3.9), odparite do suhega z rahlim segrevanjem v blagem toku dušika, ponovno dolijte nekaj kapelj acetona (točka 4.8) in spet odparite do suhega.
The residue contained in the test tube must consist of the sterol and triterpene dialchols fractions.
Ostanek v epruveti mora biti sestavljen iz sterolnih in triterpenskih dialkoholnih frakcij.
Preparation of the trimethylsilyl ethers.
Priprava trimetilsililnih etrov
Add the silylation reagent (point 4.25) (Note 6), in the ratio of 50 μl for every milligram of sterols and triterpene dialcohols, in the test tube containing the sterol and triterpene fraction, avoiding any uptake of moisture (Note 7).
Reagent za sililiranje (točka 4.25) (opomba 6), v razmerju 50 μl za vsak miligram sterolov in triterpenskih dialkoholov, dodajte v epruveto, ki vsebuje sterolsko in triterpensko dialkoholno frakcijo, tako da se izognete kakršnemu koli vnosu vlage (opomba 7).
Ready for use solutions are available commercially.
Raztopine, pripravljene za uporabo, se dobijo v prodaji.
Other silylation reagents, such as, for example, bistrimethylsilyl trifluor acetamide + 1 % trimethylchlorosilane, which has to be diluted with an equal volume of anhydrous pyridine, are also available.
Na voljo so tudi drugi reagenti za sililiranje, kot je na primer bis-trimetilsilil trifluoroacetamid + 1-odstoten trimetilklorosilan, ki ga je treba razredčiti z enako količino brezvodnega piridina.
Pyridine can be replaced by the same amount of acetonitrile.
Piridin se lahko zamenja z enako količino acetonitrila.
Stopper the test tube, shake carefully (without overturning) until the compounds are completely dissolved.
Zamašite epruveto in jo previdno pretresite (ne da bi jo prevračali), dokler niso sestavine popolnoma raztopljene.
Leave to stand for at least 15 minutes at ambient temperature and then centrifuge for a few minutes.
Pustite jo pri sobni temperaturi najmanj 15 minut, nato pa nekaj minut centrifugirajte.
The clear solution is ready for gas chromatographic analysis.
Prozorna raztopina je pripravljena za plinskokromatografsko analizo.
The slight opalescence, which may form, is normal and does not cause any anomaly.
Rahla opalescenca, ki lahko nastane, je normalna in ne povzroča nobenih nepravilnost.
The formation of a white flock or the appearance of a pink colour is indicative of the presence of moisture or deterioration of the reagent.
Tvorjenje belega flokulata ali pojav rožnate obarvanosti sta znaka prisotnosti vlage ali pokvarjenosti reagenta.
If this occurs, the test must be repeated (only if hexamethyldisilazane/trimethylchlorosilane is used).
V takem primeru je treba preskus ponoviti (le če se uporabi heksametildisilizan/trimetilklorosilan).
Gas chromatographic analysis.
Plinskokromatografska analiza
Preliminary operations, capillary column conditioning.
Predhodni postopki, kondicioniranje kapilarne kolone
Fit the column (point 3.11) in the gas chromatograph, by attaching the inlet end to the split injector and the outlet end to the detector.
Kolono (točka 3.11) pritrdite na plinski kromatograf, in sicer vstopni del na injektor z deljenjem vzorca, izhodnega pa na detektor.
Carry out general checks on the gas chromatograph unit (leaks from the gas circuits, detector efficiency, efficiency of the splitting system and recording system, etc.).
Opravite splošne preglede plinskega kromatografa (tesnjenje tokokroga plinov, učinkovitost detektorja, učinkovitost sistema za vbrizgavanje z deljenjem vzorca in sistema beleženja itd.).
If the column is being used for the first time, it is recommended that it be subjected to conditioning: passing a gentle flow of gas through the column itself, then switch on the gas chromatography unit and begin a gradual heating, up to a temperature of at least 20 °C above the operating temperature (Note 8).
Če kolono uporabljate prvič, je priporočljivo, da jo kondicionirate, in sicer tako, da skozi kapilarno kolono spustite blag tok plina, nato pa vključite plinski kromatograf in začnete postopno segrevanje do temperature najmanj 20 °C nad delovno temperaturo (opomba 8).
Hold this temperature for at least two hours, then place the entire unit in operating mode (adjustment of gas flows and splitting, ignition of the flame, connection with the computing system, adjustment of the column, detector and injector temperature, etc.) and then record the signal with a sensitivity at least two times greater than that one intended for the analysis.
To temperaturo vzdržujte najmanj dve uri, nato pa vzpostavite delovne pogoje (nastavitev pretoka plina in deljenja vzorca, vžig plamena, priključitev na računalniški sistem, prilagoditev temperature kolone, detektorja in injektorja itd.) in beležite signal z občutljivostjo, ki je najmanj dvakrat večja od tiste, ki je predvidena za analize.
The course of the base line must be linear, without peaks of any kind, and must not show drift.
Potek bazne linije mora biti linearen, brez kakršnih koli vrhov, in ne sme imeti odklona.
A negative straight-line drift indicates leakage from the column connections; a positive drift indicates inadequate conditioning of the column.
Negativen premočrtni odklon kaže na slabo tesnjenje med kolono in instrumentom, pozitiven odklon pa na slabo kondicionirano kolono.
Note 8:
Opomba 8:
The conditioning temperature must always be at least 20 °C less than the maximum temperature specified for the stationary phase used.
Temperatura kondicioniranja mora vedno biti najmanj 20 °C nižja od najvišje temperature, določene za uporabljeno stacionarno fazo.
Choice of operating conditions.
Izbor delovnih pogojev
The operating conditions are as follows:
Delovni pogoji so naslednji:
Column temperature: 260 ± 5 °C;
temperatura kolone: 260 ± 5 °C,
Injector temperature: 280-300 °C;
temperatura injektorja: 280–300 °C,
Detector temperature: 280-300 °C;
temperatura detektorja: 280–300 °C,
Linear velocity of the carrier gas: helium 20 to 35 cm/s; hydrogen 30 to 50 cm/s;
linearna hitrost nosilnega plina: 20 do 35 cm/s za helij, 30 do 50 cm/s za dušik,
Splitting ratio: from 1:50 to 1:100;
delilno razmerje: od 1: 50 do 1: 100,
Instrument sensitivity: from 4 to 16 times the minimum attenuation;
občutljivost instrumenta: 4- do 16-kratna minimalna atenuacija,
Recording sensitivity: 1 to 2 mV full scale;
občutljivost beleženja: 1 do 2 mV celotne skale,
Amount of substance injected: 0,5 to 1 μl of TMSE solution.
količina vbrizgane snovi: 0,5 do 1 μl raztopine TMSE.
These conditions may be changed according to the characteristics of the column and gas chromatograph, so as to obtain chromatograms, which meet the following requirements:
Ti pogoji se lahko spremenijo glede na značilnosti kolone in plinskega kromatografa, tako da se dobijo kromatogrami, ki izpolnjujejo naslednje zahteve:
The retention time for the ß-sitosterol peak should be at 20 ± 5 min;
retenzijski čas β-sitosterola mora biti 20 ± 5 minut,
The campesterol peak should be: for olive oil (mean content 3 %) 20 ± 5 % of full scale; for soybean oil (average content 20 %) 80 ± 10 % of full scale;
vrh za kampesterol mora biti: za oljčno olje (povprečna vsebnost 3 %) 20 ± 5 % celotne skale; za sojino olje (povprečna vsebnost 20 %) 80 ± 10 % celotne skale,
All the present sterols must be separated.
vsi prisotni steroli morajo biti ločeni.
In addition to being separated the peaks, they must also be completely resolved, i.e. the peak trace should return to the base line before leaving for the next peak.
Poleg tega, da so ločeni, morajo biti vrhovi tudi popolnoma razdvojeni, tj. signal vrha se mora pred začetkom novega vrha vrniti na bazno linijo.
Incomplete resolution is, however, tolerated, provided that the peak at RRT 1,02 (Sitostanol) can be quantified using the perpendicular.
Vendar je nepopolna resolucija sprejemljiva pod pogojem, da se vrh pri TRR 1.02 (sitostanol) lahko količinsko opredeli s pomočjo pravokotnice.
Analytical procedure
Analizni postopek
By using the 10 μl microsyringe, take 1 μl of hexane, draw in 0,5 μl of air and then 0,5 to 1 μl of the sample solution.
Z 10 μl mikrobrizgalko odmerite 1 μl heksana, 0,5 μl zraka in 0,5 do 1 μl vzorčne raztopine.
Raise the plunger of the syringe further, so the needle is emptied.
Dvignite bat mikrobrizgalke, tako da se igla izprazni.
Push the needle through the membrane of the injector and after one to two seconds, inject rapidly, and then slowly remove the needle after around five seconds.
Iglo potisnite skozi membrano injektorja in po eni do dveh sekundah raztopino hitro vbrizgajte in potem po približno petih sekundah iglo počasi izvlecite.
An automatic injector can be used as well.
Uporabi se lahko tudi avtomatski injektor.
Carry out the recording until the TMSE of the present triterpene dialcohols are completely eluted.
Beležite, dokler TMSE prisotnih triterpenskih dialkoholov popolnoma ne eluirajo.
The base line must continue to meet the requirements (point 5.4.1.2).
Bazna linija mora vedno ustrezati zahtevam (točka 5.4.1.2).
Identify individual peaks on the basis of retention times and by comparison with the mixture of sterol and triterpene dialcohols TMSE, analysed under the same conditions (see Appendix).
Identificirajte posamezne vrhove na podlagi retenzijskih časov in s primerjavo z mešanico sterolnih in triterpenskih dialkoholnih TMSE, analiziranih v enakih pogojih (glej Dodatek).
The sterols and triterpene dialcohols are eluted in the following order: cholesterol, brassicasterol, ergosterol, 24-methylen-cholesterol, campesterol, campestanol, stigmasterol, Δ7-campesterol, Δ5,23-stigmastadienol, clerosterol, ß-sistosterol, sitostanol, Δ5-avenasterol, Δ5,24-stigmastadienol, Δ7-stigmastenol, Δ7-avenasterol, erythrodiol and uvaol.
Steroli in triterpenski dialkoholi eluirajo v naslednjem zaporedju: holesterol, brasikasterol, ergosterol, 24-metilen holesterol, kampesterol, kampestanol, stigmasterol, Δ7-kampesterol, Δ5,23-stigmastadienol, klerosterol, ß-sistosterol, sitostanol, Δ5-avenasterol, Δ5,24-stigmastadienol, Δ7-stigmastenol, Δ7-avenasterol, eritrodiol in uvaol.
The retention times for ß-sitosterol, for SE-52 and SE-54 columns, are shown in Table 1.
Retenzijska časa za ß-sitosterol za koloni SE-52 in SE-54 sta prikazana v tabeli 1.
Figures 1 and 2 show typical chromatograms for some oils.
Sliki 1 in 2 prikazujeta tipične kromatograme nekaterih olj.