natgillin/translations-raw huggingface dataset:
Alizarin Red solution (dilute 2,5 ml 1M hydrochloric acid in 100 ml water and add 200 mg Alizarin Red to this solution)
Raztopina rdečega alizarina (2,5 ml 1M klorovodikove kisline se raztopi v 100 ml vode in tej raztopini se doda 200 mg rdečega alizarina.)
Mounting media
Sredstva za pripravo preparatov
Lye (NaOH 2,5 % w/v or KOH 2,5 % w/v)
Lug (NaOH 2,5 % m/v ali KOH 2,5 % m/v)
Glycerol (undiluted, viscosity: 1490 cP)
Glicerol (nerazredčen, viskoznost: 1490 cP)
Norland ® Optical Adhesive 65 (viscosity: 1200 cP) or a resin with equivalent properties for permanent slide preparation
Norland Optical Adhesive 65® (viskoznost: 1200 cP) ali smola z enakovrednimi lastnostmi za pripravo trajnih preparatov
Mounting media with staining properties
Sredstva za pripravo preparatov z lastnostmi obarvanja
Lugol solution (dissolve 2 g potassium iodide in 100 ml water and add 1 g iodine while frequently shaking)
Lugolova raztopina (2 g kalijevega jodida se raztopi v 100 ml vode in med pogostim stresanjem se doda 1 g joda)
Cystine reagent (2 g lead acetate, 10 g NaOH/100 ml water)
Cistinski reagent (2 g svinčevega acetata, 10 g NaOH/100 ml vode)
Fehling’s reagent (prepared before use from equals parts (1/1) of two stock solutions A and B. Solution A: dissolve 6,9 g copper (II) sulphate pentahydrate in 100 ml water. Solution B: dissolve 34,6 g potassium sodium tartrate tetrahydrate and 12 g NaOH in 100 ml water)
Fehlingov reagent (pripravi se pred uporabo iz enakih delov (1/1) osnovnih raztopin A in B. Raztopina A: 6,9 g bakrovega (II) sulfata pentahidrata se raztopi v 100 ml vode. Raztopina B: 34,6 g kalij-natrijevega tartrata tetrahidrata in 12 g NaOH se raztopi v 100 ml vode)
Ethanol ≥ 96 % (technical grade)
Etanol ≥ 96 % (tehnično čist)
Acetone (technical grade)
Aceton (tehnično čist)
Commercial sodium hypochlorite solution (9 - 14 % active chlorine)
Komercialna raztopina natrijevega hipoklorita (9–14 % aktivnega klora)
Analytical balance with an accuracy of 0,001 g
Analitska tehtnica, natančna na 0,001 g
Grinding equipment:
Oprema za mletje:
mill - or mortar
mlin ali terilnica
Sieves with square meshes of 0,25 mm and 1 mm width
Siti z mrežico s kvadratnimi odprtinami širine 0,25 mm in 1 mm
Conical glass separation funnel with a content of 250 ml with Teflon or ground glass stopcock at the base of the cone.
Konični stekleni lij ločnik s prostornino 250 ml in teflonskim petelinčkom ali petelinčkom iz brušenega stekla na dnu konusa.
Stopcock opening diameter shall be ≥ 4mm.
Premer odprtine petelinčka mora biti ≥ 4 mm.
Alternatively, a conical bottomed settling beaker may be used provided the laboratory has demonstrated that detection levels are equivalent to that obtained using the conical glass separation funnel.
Namesto tega se lahko uporabi konična čaša za usedlino, če laboratorij dokaže, da je meja zaznavnosti enakovredna tisti pri uporabi koničnega steklenega lija ločnika.
Separation funnel
Lij ločnik
Stereomicroscope covering at least a 6,5× to 40× final magnification range
Stereomikroskop, ki zajema vsaj končno območje povečave 6,5× do 40×.
Compound microscope covering at least a 100× to 400× final magnification range with transmitted light bright field.
Sestavljeni mikroskop, ki zajema vsaj končno območje povečave 100× do 400× in ima svetlo polje za presevno osvetlitev.
Polarised light and differential interferential contrast can additionally be used
Dodatno se lahko uporabita polarizirana svetloba in diferenčni interferenčni kontrast.
Standard laboratory glassware
Standardna laboratorijska steklovina
Equipment for slide preparation:
Oprema za pripravo preparata:
classical microscope slides, hollow slides, coverslips (20 × 20 mm), tweezers, fine spatula
klasična mikroskopska objektna stekelca, stekelca z vdolbino, krovna stekelca (20 × 20 mm), pincete, fina lopatica
Sampling and sample preparation
Vzorčenje in priprava vzorcev
A representative sample, taken in accordance with the provisions laid down in Annex I shall be used.
Uporabi se reprezentativni vzorec, ki je bil odvzet v skladu z določbami iz Priloge I.
In order to avoid laboratory cross-contamination, all reusable equipment shall be carefully cleaned before use.
Za preprečitev navzkrižnega onesnaženja je treba laboratorijsko opremo za večkratno uporabo temeljito očistiti pred uporabo.
Separation funnel pieces shall be disassembled before cleaning.
Dele lija ločnika je treba pred čiščenjem razstaviti.
Separation funnel pieces and glassware shall be pre-washed manually and then washed in a washing machine.
Sestavine lija ločnika in vso drugo steklovino je treba najprej očistiti ročno in nato še v pomivalnem stroju.
Sieves shall be cleaned by using a brush with stiff synthetic hairs.
Sita je treba očistiti s krtačo s trdimi sintetičnimi ščetinami.
A final cleaning of sieves with acetone and compressed air is recommended after sieving of fatty material like fishmeal.
Končno čiščenje sit z acetonom in stisnjenim zrakom se priporoča po sejanju mastnega materiala, kot je ribja moka.
Preparation of samples other than fat or oil
Priprava vzorcev, razen vzorcev, ki vsebujejo maščobe ali olja
Sample drying:
Sušenje vzorca:
samples with a moisture content > 14 % shall be dried prior to handling.
vzorci z vsebnostjo vlage več kot 14 % se pred obdelavo posušijo.
Sample pre-sieving:
Predhodno presejanje vzorca:
it is recommended to pre-sieve at 1 mm pelleted feeds and kernels and to subsequently prepare and analyse the two resulting fractions as distinct samples.
priporoča se, da se krma v briketih in zrnu predhodno preseje na 1 mm, nato pa se dobljeni frakciji analizirata kot ločena vzorca.
Sub-sampling and grinding:
Podvzorčenje in mletje:
at least 50 g of the sample shall be sub-sampled for analysis and subsequently ground.
iz najmanj 50 g vzorca se pripravi podvzorec za analizo, ki se nato zmelje.
Extraction and preparation of the sediment:
Ekstrakcija in priprava usedline:
a portion of 10 g (accurate to 0,01 g) of the ground sub-sample shall be transferred into the separation funnel or conical bottomed settling beaker and 50 ml of tetrachloroethylene shall be added.
ramski vzorec (na 0,01 g natančno) iz zmletega podvzorca se prenese v lij ločnik ali konično čašo za usedlino in se mu doda 50 ml tetrakloroetilena.
The portion transferred into the funnel shall be limited to 3 g in case of fishmeal or other pure animal products, mineral ingredients or premixes which generate more than 10 % of sediment.
Vzorec, ki se prenese v lij, tehta največ 3 g, kadar vzorec tvorijo ribja moka ali drugi čisti živalski proizvodi, mineralne sestavine ali premiksi, ki tvorijo več kot 10 % usedline.
The mixture shall be vigorously shaken for at least 30 s and at least 50 ml more of tetrachloroethylene shall be added cautiously while washing down the inside surface of the funnel to remove any adhering particles.
Mešanica naj se močno stresa vsaj 30 sekund in nato previdno doda še najmanj 50 ml tetrakloroetilena med spiranjem notranje površine lija za odstranitev vseh pritrjenih delcev.
The resulting mixture shall be left to stand for at least 5 minutes before the sediment is separated off by opening the stopcock.
Tako dobljena mešanica se pusti stati vsaj 5 minut, nato se usedlino loči z odprtjem petelinčka.
If a conical bottomed settling beaker is used then the mixture shall be vigorously stirred for at least 15 s and any particles adhering to the side of the beaker shall be carefully washed down the inside surface with at least 10 ml of clean tetrachloroethylene.
Če se uporabi konična čaša za usedlino, se mešanica močno meša vsaj 15 sekund in vsi delci, ki se držijo čaše, se skrbno sperejo po notranji površini z najmanj 10 ml čistega tetrakloroetilena.
The mixture shall be left to stand for 3 minutes and then stirred again for 15 seconds and any particles adhering to the side of the beaker shall be carefully washed down the inside surface with at least 10 ml of clean tetrachloroethylene.
Mešanica se pusti stati 3 minute in nato se ponovno meša 15 sekund, vsi delci, ki se držijo čaše, pa se skrbno sperejo po notranji površini z najmanj 10 ml čistega tetrakloroetilena.
The resulting mixture shall be left to stand for at least 5 minutes and then the liquid fraction is removed and discarded by careful decanting, taking care not to lose any of the sediment.
Tako dobljena mešanica se pusti stati vsaj 5 minut, nato se tekoča frakcija pazljivo dekantira in zavrže, da se usedlina v celoti ohrani.
The sediment shall be dried and subsequently weighed (accurate to 0,001 g).
Usedlina se posuši in nato stehta (na 0,001 g natančno).
If more than 5 % of the sediment consists of particles > 0,50 mm, it shall be sieved at 0,25 mm and the two resulting fractions shall be examined.
Če več kot 5 % usedline sestavljajo delci velikosti > 0,50 mm, se preseje skozi sito 0,25 mm, tako pridobljeni frakciji se nato analizirata.
Extraction and preparation of the flotate:
Ekstrakcija in priprava flotata:
after recovery of the sediment with the method described above, two phases should remain in the separation funnel:
po pridobitvi usedline z opisano metodo ostaneta v liju ločniku dve fazi:
a liquid one consisting of tetrachloroethylene and a solid one made of floating material.
tekoča faza, ki jo tvori tetrakloroetilen, in trdna faza, ki jo tvori plavajoči material.
This solid phase is the flotate and shall be recovered by pouring off completely tetrachloroethylene from the funnel by opening the stopcock.
Ta trdna faza je flotat, ki se izolira tako, da se tetrakloroetilen v celoti odlije iz lija z odprtjem petelinčka.
By inverting the separation funnel, the flotate shall be transferred into a large Petri dish and air dried in a fumehood.
Z obrnitvijo lija ločnika na glavo se flotat prenese v veliko petrijevko in posuši na zraku v digestoriju.
If more than 5 % of the flotate consists of particles > 0,50 mm, it shall be sieved at 0,25 mm and the two resulting fractions shall be examined.
Če več kot 5 % flotata sestavljajo delci velikosti > 0,50 mm, se preseje skozi sito 0,25 mm, tako pridobljeni frakciji se nato analizirata.
Preparation of raw material:
Priprava osnovnega materiala:
a portion of at least 5 g of the ground sub-sample shall be prepared.
pripravi se vzorec iz najmanj 5 g zmletega podvzorca.
If more than 5 % of the material consists of particles > 0,50 mm, it shall be sieved at 0,25 mm and the two resulting fractions shall be examined.
Če več kot 5 % materiala sestavljajo delci velikosti > 0,50 mm, se preseje skozi sito 0,25 mm, tako pridobljeni frakciji se nato analizirata.
Preparation of samples consisting of fat or oil
Priprava vzorcev, ki vsebujejo maščobe ali olja
The following protocol shall be followed for the preparation of samples consisting of fat or oil:
Za pripravo vzorcev, ki vsebujejo maščobe ali olja, se uporabi naslednji protokol:
if the fat is solid, it shall be warmed in a oven until it is liquid.
če je maščoba trdna, se segreje v pečici, dokler ne postane tekoča,
by using a pipette, 40 ml of fat or oil shall be transferred from the bottom of the sample to a centrifugation tube.
40 ml maščobe ali olja z dna vzorca se odpipetira v centrifugirko,
centrifuge during 10 minutes at 4000 r.p.m.
centrifugira se 10 minut pri 4000 obr./min.,
if the fat is solid after centrifugation, it shall be warmed in an oven until it is liquid.
če je maščoba po centrifugiranju trdna, se segreje v pečici, dokler ne postane tekoča,
repeat the centrifugation during 5 minutes at 4000 r.p.m.
ponovno se centrifugira 5 minut pri 4000 obr./min.,
by using a small spoon or a spatula, one half of the decanted impurities shall be transferred to microscopic slides for examination, Glycerol is recommended as mounting medium.
z majhno žličko ali lopatico se polovica dekantirane nečistoče prenese na mikroskopska objektna stekelca za pregled, kot sredstvo za pripravo preparata pa se priporoča glicerol,
the remaining impurities shall be used for preparing the sediment as described in point 2.1.3.3.
preostale nečistoče se uporabijo za pripravo usedline, kot je opisano v točki 2.1.3.3.
Use of staining reagents
Uporaba reagentov za obarvanje
In order to facilitate the correct identification of the constituents of animal origin, the operator may use staining reagents during the sample preparation in accordance with guidelines issued by the EURL-AP and published on its website.
Da se omogoči pravilna identifikacija sestavin živalskega izvora, lahko izvajalec med pripravo vzorca uporabi reagente za obarvanje v skladu s smernicami, ki jih je izdal in na svoji spletni strani objavil EURL-AP.
In case Alizarin Red solution is used to colour the sediment, the following protocol shall apply:
Če se za barvanje usedline uporabi raztopina rdečega alizarina, se uporabi naslednji protokol:
the dried sediment shall be transferred into a glass test tube and rinsed twice with approximately 5 ml of ethanol (each time a vortex of 30 s shall be used, the solvent shall be let settle about 1 min 30 s and poured off).
posušena usedlina se prenese v stekleno epruveto in dvakrat spere s približno 5 ml etanola (vsakič se uporabi vorteks mešalnik za 30 sekund, topilo se pusti stati približno 1 minuto in 30 sekund ter se nato odtoči),
the sediment shall be bleached by adding at least 1 ml sodium hypochlorite solution.
usedlina se pobeli z dodatkom najmanj 1 ml raztopine natrijevega hipoklorita.
The reaction shall be allowed to continue for 10 min. The tube shall be filled with water, the sediment shall be let settle 2-3 min, and the water and the suspended particles shall be poured off gently.
Čas reakcije je 10 minut. Epruveta se napolni z vodo, usedlina se pusti stati 2 do 3 minute, voda in suspendirani delci pa se previdno odtočijo,
the sediment shall be rinsed twice more with about 10 ml of water (a vortex shall be used for 30 s, let settle, and pour off the water each time).
usedlina se dvakrat spere z 10 ml vode (vsakič se za 30 sekund uporabi vorteks mešalnik, pusti se stati in odtoči se voda),
2 to 10 drops of the Alizarin Red solution shall be added and the mixture shall be vortexed.
doda se 2 do 10 kapljic raztopine rdečega alizarina in mešanica se premeša v vorteks mešalniku.
The reaction shall be let occur for 30 s and the coloured sediment shall be rinsed twice with approximately 5 ml ethanol followed by one rinse with acetone (each time a vortex of 30 s shall be used, the solvent shall be let settle about 1 min and poured off).
Čas reakcije je 30 sekund, obarvana usedlina se dvakrat spere s približno 5 ml etanola, nato pa enkrat z acetonom (vsakič se uporabi vorteks mešalnik za 30 sekund, topilo se pusti stati približno 1 minuto ter se nato odtoči),
the coloured sediment shall be dried.
obarvana usedlina se posuši.
Slide preparation
Priprava preparata
Microscopic slides shall be prepared from the sediment and, depending of operator’s choice, from either the flotate or the raw material.
Mikroskopski preparat se pripravi iz sedimenta in, odvisno od izbire izvajalca, flotata ali osnovnega materiala.
In case sieving has been used during the sample preparation, the two resulting fractions (the fine and the coarse one) shall be prepared.
Če je bilo med pripravo vzorca uporabljeno sejanje, se pripravita obe tako pridobljeni frakciji (fina in groba).
Test portions of fractions spread on slides shall be representative of the whole fraction.
Testni vzorci frakcij, ki se nanesejo na objektna stekelca, so reprezentativni za celotno frakcijo.
A sufficient number of slides shall be prepared in order to ensure that a complete examination protocol as laid down in point 2.1.4.2 can be carried-out.
Pripraviti je treba zadostno število preparatov, da se lahko zagotovi izvedba celotnega protokola pregleda, kot je določen v točki 2.1.4.2.
Microscopic slides shall be mounted with the adequate mounting medium in accordance with the SOP established by the EURL-AP and published on its website.
Mikroskopski preparati se pripravijo s primernim sredstvom za pripravo preparata v skladu s standardnimi operativnimi postopki, ki jih je določil in na svoji spletni strani objavil EURL-AP.
The slides shall be covered with coverslips.
Preparati se pokrijejo s krovnimi stekelci.
Observation protocols for the detection of animal particles in compound feed and feed material
Protokol pregleda za določanje živalskih delcev v krmnih mešanicah in posamičnih krmilih
The prepared microscopic slides shall be observed in accordance with the observation protocols laid down in diagram 1 for compound feed and feed materials other than pure fishmeal, or in diagram 2 for pure fishmeal.
Pripravljeni mikroskopski preparati se pregledajo v skladu s protokolom pregleda, prikazanim v diagramu 1 za krmne mešanice in posamična krmila, razen za čisto ribjo moko, za katero je prikazan v diagramu 2.
The microscopic observations shall be conducted using the compound microscope on the sediment and, depending of the operator’s choice, either on the flotate or on the raw material.
Mikroskopski preparat sedimenta in, odvisno od izbire izvajalca, flotata ali osnovnega materiala se pregleda s sestavljenim mikroskopom.
The stereomicroscope may be used in addition to the compound microscope for the coarse fractions.
Za grobe frakcije se lahko poleg sestavljenega mikroskopa uporabi stereomikroskop.
Each slide shall be screened entirely at various magnifications.
Vsak preparat se pregleda v celoti pri različnih povečavah.
The minimum numbers of slides to be observed at each step of the observation protocol shall be strictly respected unless the entire fraction material does not permit to reach the stipulated slide number.
Pri vsakem koraku protokola pregleda je treba pregledati določeno minimalno število preparatov, razen če celotna frakcija materiala ne omogoča, da se pripravi zahtevano število preparatov.
No more than 6 slides per determination shall be observed.
Pri vsakem določanju se pregleda največ šest preparatov.
In order to facilitate the identification of the particles’ nature and origin, the operator may use support tools like decision support systems, image libraries and reference samples.
Za lažjo identifikacijo narave in izvora delcev lahko izvajalec uporabi podporne elemente, na primer sisteme za podporo pri odločanju, slikovne knjižnice in referenčne vzorce.
Diagram 1
Diagram 1:
Observation protocol for the detection of animal particles in compound feed and feed material other than fishmeal
protokol pregleda za določanje živalskih delcev v krmnih mešanicah in posamičnih krmilih, razen v ribji moki
Grinding process of sample insures 95 % of particles ≤ 0.50 mm
postopek mletja vzorca zagotavlja, da je 95 % delcev ≤ 0,50 mm
Sieving on 0.25 mm mesh
sejanje na sitih z mrežico s kvadratnimi odprtinami širine 0,25 mm
3 slides from sediment + 1 slide from flotate or raw material
3 preparati iz sedimenta + 1 preparat iz flotata ali osn. materiala
3 slides from < 0.25 mm sediment + 1 slide < 0.25 mm flotate or raw material
3 preparati iz sedimenta z delci < 0,25 mm + 1 preparat iz flotata ali osn. materiala z delci < 0,25 mm
> 5 animal particles of same nature detected?
> 5 ugotovljenih živalskih delcev iste narave?
sample declared according to point 2.1.5.3 for FISH
vzorec določen v skladu s točko 2.1.5.3 za RIBO
repetition of analysis according to point 2.1.4.3
ponovitev analize v skladu s točko 2.1.4.3
Number of fish particles?
število ribjih delcev?
1 slide from sediment
1 preparat iz sedimenta
FISH
RIBA
1 slide from > 0.25 mm sediment
1 preparat iz sedimenta z delci > 0,25 mm
nature of animal particles?
narava živalskih delcev?
TERRESTRIAL
KOPENSKA ŽIVAL
1 slide from rotate or raw material
1 preparat iz flotata ali osn. materiala
Number of terrestrial particles?
število delcev kopenskih živali?
1 slide from > 0.25 mm flotate or raw material
1 preparat iz flotata ali osn. materiala z delci > 0,25 mm
animal particles detected?
ugotovljeni živalski delci?
sample declared according to point 2.1.5.3 for TERRESTRIAL ANIMAL
vzorec določen v skladu s točko 2.1.5.3 za KOPENSKO ŽIVAL
sample declared according to point 2.1.5.1
vzorec določen v skladu s točko 2.1.5.1
Diagram 2
Diagram 2:
Observation protocol for the detection of animal particles in fishmeal
protokol pregleda za določanje živalskih delcev v ribji moki
Grinding process insures 95% of particles ≤ 0.50 mm
postopek mletja vzorca zagotavlja, da je 95 % delcev ≤ 0,50 mm
3 slides from sediment
3 preparati iz sedimenta
3 slides from < 0.25 mm sediment
3 preparati iz sedimenta z delci < 0,25 mm
> 5 terrestrial particles detected?
> 5 ugotovljenih delcev kopenskih živali?
2 slides from sediment
2 preparata iz sedimenta
2 slides from > 0.25 mm sediment
2 preparata iz sedimenta z delci > 0.25 mm
1 slide from flotate or raw material
1 preparat iz flotata ali osn. materiala
1 slide from < 0.25 mm flotate or raw material
1 preparat iz flotata ali osn. materiala z delci < 0,25 mm
terrestrial particles detected?
ugotovljeni delci kopenskih živali?
sample declared according to point 2.1.5.1 for TERRESTRIAL ANIMAL
vzorec določen v skladu s točko 2.1.5.1 za KOPENSKO ŽIVAL
If following a first determination carried out in accordance with the observation protocol laid down in diagram 1 or diagram 2 as relevant, no animal particle of a given nature (i.e. terrestrial animal or fish) is detected, no additional determination is necessary and the result of the analysis shall be reported using the terminology laid down in point 2.1.5.1.
Če pri prvem določanju, izvedenem v skladu s protokolom iz diagrama 1 ali diagrama 2, ni ugotovljen noben živalski delec danega izvora (tj. od kopenskih živali ali rib), ni potrebno dodatno določanje, rezultate analize pa je treba sporočiti z uporabo terminologije iz točke 2.1.5.1.
If, following a first determination carried out in accordance with the observation protocols laid down in diagram 1 or in diagram 2 as relevant, the total number of animal particles of a given nature (i.e. terrestrial animal or fish) detected ranges from 1 to 5, a second determination shall be performed from a new 50 g sub-sample.
Če je bilo pri prvem določanju, izvedenem v skladu s protokolom iz diagrama 1 ali diagrama 2, skupno ugotovljenih od 1 do 5 živalskih delcev danega izvora (tj. od kopenskih živali ali rib), se izvede drugo določanje na novem 50-gramskem podvzorcu.
If, following this second determination, the number of animal particles of this given nature detected ranges from 0 to 5, the result of the analysis shall be reported using the terminology laid down in point 2.1.5.2., else a third determination shall be carried out from a new 50 g sub-sample.
Če je bilo pri drugem določanju ugotovljenih od 0 do 5 živalskih delcev danega izvora, je treba rezultat analize sporočiti z uporabo terminologije iz točke 2.1.5.2., sicer se izvede tretje določanje na novem 50-gramskem podvzorcu.
Nevertheless, if following the first and the second determination, the sum of the particles of a given nature detected over the two determinations is higher than 15, no additional determination is necessary and the result of the analysis shall be directly reported using the terminology laid down in point 2.1.5.3.
Če pa je po prvem in drugem določanju vsota ugotovljenih delcev danega izvora višja od 15, dodatno določanje ni potrebno, rezultate analize pa je treba neposredno sporočiti z uporabo terminologije iz točke 2.1.5.3.
If, following the third determination, the sum of the animal particles of a given nature detected over the three determinations is higher than 15, the result of the analysis shall be reported using the terminology laid down in point 2.1.5.3.
Če je po tretjem določanju vsota ugotovljenih živalskih delcev danega izvora višja od 15, je treba rezultate analize sporočiti z uporabo terminologije iz točke 2.1.5.3.
Otherwise, the result of the analysis shall be reported using the terminology laid down in point 2.1.5.2.
Sicer je treba rezultate analize sporočiti z uporabo terminologije iz točke 2.1.5.2.