natgillin/translations-raw huggingface dataset:
Percentage inhibition (PI) value = 100 – (OD of each test control/Mean OD of Cm) × 100.
Vrednost odstotka inhibicije (PI) = 100 – (OD vsake preiskovane kontrole/srednja vrednost OD kontrole za monoklonsko protitelo (Cm)) × 100.
The duplicate negative control serum wells and the duplicate blank wells must record PI values between +25 % and –25 %, and between +95 % and +105 %, respectively.
Dvojniki jamic z negativnim kontrolnim serumom in dvojniki praznih jamic morajo prikazati vrednosti PI med +25 % in –25 % oziroma med +95 % in +105 %.
Failure to be within these limits does not invalidate the plate but does suggest that background colour is developing.
Če vrednosti niso znotraj predpisanih meja, plošča ni neveljavna, da pa slutiti, da se razvija barva ozadja.
The strong and weak positive control sera must record PI values between +81 % and +100 %, and between +51 % and +80 %, respectively.
Močno in šibko pozitivni kontrolni serumi morajo prikazati vrednosti PI med +81 % in +100 % oziroma med +51 % in +80 %.
The diagnostic threshold for test sera is 50 % (PI 50 % or OD 50 %).
Diagnostični prag za testne serume je 50 % (PI 50 % ali OD 50 %).
Samples recording PI values >50 % are recorded negative.
Vzorci, ki prikažejo vrednosti PI < 50 %, se ocenijo kot negativni.
Samples that record PI values above and below the threshold for the duplicate wells are considered doubtful; such samples may be re-tested in the spot test and/or titration.
Vzorci z vrednostmi PI nad pragom dvojnikov jamice in pod njim se ocenijo kot dvomljivi; takšne vzorce se mora ponovno testirati z enojnim redčenjem in/ali titracijo.
Visual reading: Positive and negative samples are easily discernible by eye; weakly positive or strong negative samples may be more difficult to interpret by eye.
Vizualno odčitanje: pozitivne in negativne vzorce lahko enostavno razločimo s prostim očesom; šibko pozitivne ali močno negativne vzorce pa je težje oceniti s prostim očesom.
Preparation of BTV ELISA antigen:
Priprava antigena proti BTV za test ELISA:
Wash 40-60 roux of confluent BHK-21 cells three times with serum-free Eagle's medium and infect with bluetongue virus serotype 1 in serum-free Eagle's medium.
40-60 rouxovih stekleničk celic strnjene linije BHK-21 trikrat speremo z Eagle’s Medium brez seruma in jih inficiramo z virusom bolezni modrikastega jezika, serotip 1, v Eagle’s Medium brez seruma.
Incubate at 37 °C and examine daily for cytopathic effect (CPE).
Inkubiramo pri 37 °C in vsak dan preverimo citopatski učinek (CPE).
When CPE are complete in 90 % to 100 % of the cell sheet of each roux, harvest the virus by shaking any still-attached cells from the glass.
Ko so citopatski učinki (CPE) prisotni v 90 do 100 odstotkih v vsaki Rouxovi steklenički celične linije BHK-21, zberemo virus s stresanjem še vseh pritrjenih celic s stekla.
Centrifuge at 2000 to 3000 rpm to pellet the cells.
Centrifugiramo pri 2000 do 3000 obr./min., da skoncentriramo celice.
Discard the supernatant and re-suspend the cells in approximately 30 ml of PBS containing 1 % ‘Sarkosyl’ and 2 ml phenylmethylsulphonyl fluoride (lysis buffer).
Supernatant zavržemo in resuspendiramo celice v približno 30 ml PBS, ki vsebuje 1 % „Sarkosyla“ in 2 ml fenilmetilsulfonil fluorida (pufer za lizo celic).
This may cause the cells to form a gel and more lysis buffer may be added to reduce this effect.
Pri tem se lahko iz celic tvori gel in dodati je treba več pufra za lizo, da se zmanjša ta učinek.
(NB: phenylmethylsulphonyl fluoride is harmful - handle with extreme caution.)
(Opomba: fenilmetilsulfonil fluorid je škodljiv – z njim ravnamo izjemo previdno.)
Disrupt the cells for 60 seconds using an ultrasonic probe at an amplitude of 30 microns.
Celice razbijamo 60 sekund z ultrazvočno sondo pri amplitudi 30 mikronov.
Centrifuge at 10000 rpm for 10 minutes.
Centrifugiramo 10 minut pri 10000 vrt/min.
Store the supernatant at +4 °C and re-suspend the remaining cell pellet in 10 to 20 ml of lysis buffer.
Supernatant shranimo pri +4 °C in pripravimo resuspenzijo ostanka celičnega centrifugata v 10 do 20 ml pufra za liziranje.
Sonicate and clarify, storing the supernatant at each stage, a total of three times.
Trikrat sonificiramo in centrifugiramo ter na vsaki stopnji shranimo supernatant.
Pool the supernatants and centrifuge at 24000 rpm (100,000 g) for 120 minutes at +4 °C over a 5 ml cushion of 40 % sucrose (w/v in PBS) using 30 ml Beckmann centrifuge tubes and an SW 28 rotor.
Pripravimo zbirni vzorec supernatantov in ga centrifugiramo na Beckmannovi centrifugi (s 30-mililitrskimi epruvetami in rotorjem SW 28) pri 24000 obr./min. (100,000 g) 120 minut, pri +4 °C, na 5 mililitrski podlagi 40-odstotne raztopine saharoze (vol:vol v PBS).
Discard the supernatant, drain the tubes thoroughly and re-suspend the pellet in PBS by sonication.
Supernatant zavržemo, epruvete temeljito odcedimo in resuspendiramo centrifugat v PBS s sonificiranjem.
Store the antigen in aliquots at –20 °C.
Antigen shranimo v enakih delih pri –20 °C.
Titration of BTV ELISA antigen:
Titriranje antigena proti BTV za test ELISA:
Bluetongue ELISA antigen is titrated by the indirect ELISA.
Za titriranje antigena proti BTV se uporabi indirektna ELISA.
Twofold dilutions of antigen are titrated against a constant dilution (1/100) monoclonal antibody 3-17-A3.
Dvojne razredčine antigena titriramo glede na konstantno razredčino (1/100) monoklonalnih protiteles 3-17-A3.
The protocol is as follows:
Protokol je naslednji:
Titrate a 1:20 dilution of BTV antigen in PBS across the microtitre plate in a twofold dilution series (50 μl/well) using a multichannel pipette.
Antigen proti BTV, razredčen 1:20 v PBS, titriramo z večkanalno pipeto po vsej mikroplošči v dvojnih padajočih razredčitvah (50 μl/jamico).
Incubate for one hour at 37 °C on an orbital shaker.
Inkubiramo 1 uro pri 37 °C na orbitalnem stresalniku.
Wash plates three times with PBS.
Plošče trikrat speremo s PBS.
Add 50 μl of monoclonal antibody 3-17-A3 (diluted 1/100) to each well of the microtitre plate.
Dodamo 50 μl monoklonalnih protiteles 3-17-A3 (razredčenih 1:100) v vsako jamico na mikroplošči.
Add 50 μl of rabbit anti-mouse globulin conjugated to horseradish peroxidase, diluted to a pre-titrated optimal concentration, to each well of the microtitre plate.
Dodamo 50 μl kunčjega protimišjega globulina, konjugiranega s hrenovo peroksidazo (HRP), razredčeno na optimalno koncentracijo, v vsako jamico na mikroplošči.
Add substrate and chromogen as described previously.
Dodamo substrat in kromogen, kakor je opisano zgoraj.
Stop the reaction after 10 minutes by the addition of 1 Molar sulphuric acid (50 μl/well).
Reakcijo ustavimo po 10 minutah z dodatkom 1 molarne žveplene kisline (50 μl/jamico).
In the competitive assay, the monoclonal antibody must be in excess, therefore a dilution of antigen is chosen which falls on the titration curve (not on the plateau region) which gives approximately 0,8 OD after 10 minutes.
Pri kompetitivni ELISA morajo biti monoklonska protitelesa prisotna v pribitku in zato izberemo razredčitev antigena, katere vrednost leži na titracijski krivulji (ne na ravnini) in da približno OD vrednost 0,8 po 10 minutah.
The agar gel immuno-diffusion test shall be carried out according to the following protocol:
Agar gel imunodifuzijski test se izvaja v skladu z naslednjim protokolom:
BHK or Vero cells are recommended.
Priporočljivo je uporabiti celice BHK ali Vero.
Antigen is present in the supernatant fluid at the end of virus growth but requires 50 to 100-fold concentration to be effective. This may be achieved by any standard protein concentration procedure; virus in the antigen may be inactivated by the addition of 0,3 % (v/v) beta-propiolactone. Known positive control serum: Using the international reference serum and antigen a national standard serum is produced, standardised for optimal proportion against the international reference serum, freeze-dried and used as the known control serum in each test. Test serum Procedure 1 % agarose prepared in borate or sodium barbitol buffer, pH 8,5 to 9,0, is poured into a petri dish to a minimum depth of 3,0 mm. A test pattern of seven moisture-free wells, each 5,0 mm in diameter, is cut in the agar.
Po končani rasti virusa je antigen prisoten v tekočem supernatantu, vendar je za njegovo učinkovitost potrebna 50- do 100-kratna koncentracija.
The pattern consists of one centre well and six wells arranged round it in a circle of radius 3 cm.
To lahko dosežemo s katerim koli standardnim postopkom koncentracije proteinov; virus v antigenu lahko inaktiviramo z dodatkom 0,3 % (vol:vol) beta-propiolaktona. Znani pozitivni kontrolni serum: Ob uporabi mednarodnega referenčnega seruma in antigena pripravimo nacionalni standardni serum, standardiziran v optimalnem volumenskem razmerju glede na mednarodni referenčni serum, ga suho zamrznemo in uporabljamo kot znani kontrolni serum v vsakem testu. Testni serum
The central well is filled with the standard antigen.
Postopek 1 % agarozo, pripravljeno v boratu ali pufru natrijevega barbitola, pH 8,5 do 9,0, zlijemo v petrijevko do višine vsaj 3,0 mm. V agar vrežemo testni vzorec sedmih popolnoma suhih jamic, vsako s premerom 5,0 mm. Vzorec sestoji iz ene osrednje jamice in šestih jamic okoli nje v krogu s polmerom 3 cm. V osrednjo jamico odpipetiramo standardni antigen.
Peripheral wells 2, 4 and 6 are filled with known positive serum, wells 1, 3 and 5 are filled with test sera.
V obrobne jamice 2, 4 in 6 odpipetiramo znani pozitivni serum, testne serume pa v jamice 1, 3 in 5.
The system is incubated for up to 72 hours at room temperature in a closed humid chamber.
Tako pripravljeno ploščo inkubiramo do 72 ur pri sobni temperaturi v zaprti vlažni komori.
Interpretation A test serum is positive if it forms a specific precipitin line with the antigen and forms a complete line of identity with the control serum.
Razlaga Testni serum je pozitiven, če z antigenom oblikuje tanko specifično precipitacijsko črto in s kontrolnim serumom popolnoma ujemajočo se črto.
Epizootic haemorrhagic disease (EHD)
Epizootska hemoragična bolezen (EHD)
Precipitating antigen is prepared in any cell culture system that supports the rapid multiplication of the appropriate serotype(s) of epizootic haemorrhagic disease virus.
Precipitirajoči antigen pripravimo v kateri koli celični kulturi, v kateri se ustrezni serotip(-i) virusa epizootske hemoragične bolezni lahko hitro namnožuje(-jo).
Infectious bovine rhinotracheitis (IBR) / infectious pustular vulvo-vaginitis (IPV)
Infekciozni bovini rinotraheitis (IBR) / infekciozni pustularni vulvovaginitis (IPV)
The serum neutralisation test shall be carried out according to the following protocol:
Serum-nevtralizacijski test se izvaja v skladu z naslednjim protokolom:
Serum All sera are heat-inactivated at 56 °C for 30 minutes before use.
Serum Pred uporabo vse serume inaktiviramo 30 minut pri 56 °C.
Procedure The constant virus-varying serum neutralisation test on microtitre plates employs MDBK or other susceptible cells.
Postopek Za serum-nevtralizacijski test na mikroploščah, pri katerem se virus konstantno spreminja, uporabimo celično linijo MDBK ali druge dovzetne celice.
The Colorado, Oxford or any other reference strain of the virus is used at 100 TCID50 per 0,025 ml; inactivated undiluted serum samples are mixed with an equal volume (0,025 ml) of virus suspension.
Uporabimo Colorado, Oxford ali kateri koli drug referenčni sev virusa, pri 100 TCID50 na 0,025 ml; inaktivirane nerazredčene vzorce seruma zmešamo z enako količino (0,025 ml) suspenzije virusa.
The virus/serum mixtures are incubated for 24 hours at 37 °C in the microtitre plates before the MDBK cells are added.
Mešanice virus/serum inkubiramo 24 ur pri 37 °C na mikroploščah, preden jim dodamo MDBK celice.
Cells are used at a concentration which forms a complete monolayer after 24 hours.
Celice uporabimo v koncentraciji, ki zagotavlja, da se po 24 urah oblikuje popolna enoslojna celična kultura.
Controls (i) virus infectivity assay, (ii) serum toxicity controls, (iii) uninoculated cell culture controls, (iv) reference antisera.
Kontrole (i) preverjanje infektivnosti virusa, (ii) kontrole toksičnosti seruma, (iii) kontrole nenacepljenih celičnih kultur, (iv) referenčni antiserumi.
Interpretation The results of the neutralisation test and the titre of the virus used in the test are recorded after three to six days incubation at 37 °C.
Razlaga Rezultate serum-nevtralizacijskega testa ter titer virusa, uporabljenega v testu, odčitamo po treh do šestih dneh inkubacije pri 37 °C.
Serum titres are considered negative if there is no neutralisation at a dilution of 1/2 (undiluted serum).
Titri seruma se štejejo za negativne, če pri razredčitvi 1:2 (nerazredčeni serum) ni nevtralizacijske reakcije.
Any other test recognised in the framework of Decision 2004/558/EC [10].Foot-and-mouth disease (FMD)
Kateri koli drug test v okviru Odločbe 2004/558/ES [10].Slinavka in parkljevka (SIP)
Collecting oesophageal/pharyngeal samples and testing shall be carried out according to the following protocol:
Zbiranje ezofagealnih/faringealnih vzorcev in preskušanje se izvajata v skladu z naslednjim protokolom:
Reagents Prior to sampling, transport medium is prepared.
Reagenti Pred vzorčenjem pripravimo transportni medij.
Two ml volumes are dispensed in as many containers as there are animals to be sampled.
Po 2 ml medija damo v toliko epruvet, kolikor živali bomo vzorčili.
The containers used must withstand freezing over solid CO2 or liquid nitrogen.
Epruvete morajo biti odporne proti zamrzovanju na trdnem CO2 ali v tekočem dušiku.
Samples are obtained by the use of a specially-designed sputum collector or ’probang’.
Vzorce ezofagealnega/faringealnega sputuma zbiramo s posebno sondo, imenovano „probang“.
To obtain a sample the probang cup is passed through the mouth, over the dorsum of the tongue and down into the upper part of the oesophagus.
Za odvzem vzorca vstavimo zajemalko sonde probang skozi gobec, prek hrbta jezika, navzdol v zgornji del požiralnika.
Attempts are made to scrape the surface epithelium of the upper oesophagus and pharynx by movements directed laterally and dorsally.
Površinski epitel zgornjega dela požiralnika in žrela postrgamo lateralno in dorzalno.
The probang is then withdrawn, if possible after the animal has swallowed.
Če je mogoče, sondo probang izvlečemo po tem, ko je žival pogoltnila.
The cup must be full and contain a mixture of mucus, saliva, oesophageal fluid and cellular debris.
Zajemalka sonde probang mora biti polna in vsebovati mešanico sluzi, sline, ezofagealne tekočine in celičnega drobirja.
Care must be taken to ensure that each specimen contains some visible cellular material.
Paziti je treba, da vsak vzorec vsebuje nekaj vidnega celičnega tkiva.
Very rough handling which causes bleeding must be avoided.
Izogibati se je treba zelo grobemu ravnanju, ki povzroči krvavitev.
Samples from some animals may be heavily contaminated with ruminal contents.
Vzorci nekaterih živali so lahko močno kontaminirani z vampovo vsebino.
Such samples must be discarded and the mouth of the animal flushed with water, or preferably physiological saline, before repeat sampling.
Te vzorce je treba zavreči in gobec živali spirati z vodo ali po možnosti s fiziološko raztopino pred ponovnim vzorčenjem.
Treatmentof samples: Each sample collected in the probang cup is examined for quality and 2 ml added to an equal volume of transport medium in a container which can withstand freezing.
Priprava vzorcev Preverimo kakovost vsakega vzorca, odvzetega s sondo probang, in 2 ml vzorca z enako količino transportnega medija prelijemo v epruveto, odporno proti zamrzovanju.
The containers are tightly closed, sealed, disinfected and labelled.
Epruvete z navojem trdno zapremo, zapečatimo, razkužimo in označimo z nalepko.
The samples are kept cool (+4 °C) and examined within three to four hours or placed over dry ice (-69 °C) or liquid nitrogen and kept frozen until examined.
Vzorce ohladimo (+4 °C) in jih pregledamo po treh do štirih urah ali pa jih do pregleda hranimo zamrznjene na suhem ledu (–69 °C) ali v tekočem dušiku.
Between animals the probang is disinfected and washed in three changes of clean water.
Pred vzorčenjem vsake naslednje živali sondo probang razkužimo in trikrat speremo s čisto vodo.
Testing for FMD virus: Samples are inoculated into cultures of primary bovine thyroid cell cultures using at least three tubes per sample.
Preskusi na virus SIP Vzorce vcepimo v primarne kulture iz celic goveje ščitnice in uporabimo vsaj tri epruvete na vzorec.
Other susceptible cells such as primary bovine or porcine kidney cells can be used but it must be kept in mind that for some strains of FMD virus they are less sensitive.
Lahko uporabimo tudi druge dovzetne celice, kot so primarne celice govejih ali prašičjih ledvic, vendar je treba vedeti, da so te manj občutljive za nekatere seve virusa SIP.
The tubes are incubated at 37 °C on a roller apparatus and examined daily for 48 hours for the presence of a cytopathic effect (CPE).
Epruvete inkubiramo pri 37 °C na orbitalnem stresalniku in vsak dan v 48 urah preverjamo prisotnost citopatskega učinka (CPE).
If negative, cultures are blind passaged onto new cultures and re-examined for 48 hours.
Če je negativen, izvedemo slepo pasažo kultur na novih kulturah in ponovno preverjamo učinek v 48 urah.
The specificity of any CPE must be confirmed.
Potrditi je treba specifičnost vsakega CPE.
Recommended transport media:
Priporočeni transportni mediji:
0,08M phosphate buffer pH 7,2 containing 0,01 % bovine serum albumin, 0,002 % phenol red and antibiotics.
0,08M fosfatni pufer, pH 7,2, z 0,01 % bovinega serumskega albumina, 0,002 % fenol rdečega barvila in antibiotiki.
Tissue culture medium (such as Eagle's MEM) containing 0,04 M Hepes buffer, 0,01 % bovine serum albumin and antibiotics, pH 7,2.
Medij za tkivno kulturo (npr. Eagle’s MEM) z 0,04M pufra Hepes, 0,01 % bovinega serumskega albumina in antibiotiki, pH 7,2.
Antibiotics (per ml final) must be added to the transport medium such as penicillin1000 IU, neomycin sulphate100 IU, polymyxin B sulphate50 IU, mycostatin100 IU.
Transportnemu mediju je treba dodati antibiotike (na ml končne raztopine), npr. penicilin 1000 IE, neomicin sulfat 100 IE, polimiksin B sulfat 50 IE, mikostatin 100 IE.
The virus neutralisation test shall be carried out according to the following protocol:
Test za nevtralizacijo virusa se izvaja v skladu z naslednjim protokolom:
Reagents Stock FMDV antigen is prepared in cell cultures or on cattle tongues and stored at -70 °C or less or at -20 C after the addition of 50 % glycerol.
Reagenti Standardni antigen proti FMDV pripravimo v celičnih kulturah ali na celicah govejega jezika in ga shranimo pri –70 °C ali manj ali pri –20 °C po dodatku 50 % glicerola.
This is the stock antigen.
To je standardni antigen.
FMDV is stable under these conditions and titres vary little over a period of months.
FMDV je stabilen pri teh pogojih in titer se le malo spremeni v večmesečnem obdobju.
Procedure The test is carried out in flat-bottomed tissue culture grade microtitre plates using susceptible cells such as IB-RS-2, BHK-21 or calf kidney cells.
Postopek Test izvedemo na graduiranih mikroploščah z ravnim dnom za tkivno kulturo, v katere damo dovzetne celice, kakor so na primer IB-RS-2, BHK-21 ali celice telečje ledvice.
Sera for the test are diluted 1/4 in serum-free cell culture medium with the addition of 100 IU/ml neomycin or other suitable antibiotics.
Testne serume razredčimo v razmerju 1:4 v mediju celične kulture brez seruma, z dodatkom 100 IE/ml neomicina ali drugih primernih antibiotikov.
Sera are inactivated at 56 °C for 30 minutes and 0.05 ml amounts are used to prepare a twofold series on microtitre plates using 0,05 ml diluting loops.
Serume inaktiviramo pri 56 °C 30 minut in s količinami po 0,05 ml pripravimo na mikroploščah dvojno zaporedje razredčitvenih zank s po 0,05 ml.
Pre-titrated virus also diluted in serum-free culture medium and containing 100 TCID50/0.05 ml is then added to each well.
V vsako jamico dodamo predtitrirani virus, prav tako razredčen v mediju celične kulture brez seruma, ki vsebuje 100 TCID50 na 0,05 ml.
Following incubation at 37 °C for one hour to allow neutralisation to take place, 0,05 ml of suspension cells containing 0,5 to 1.0 × 106 cells per 1 ml in cell culture medium containing serum free of FMD antibody is added to each well and the plates are sealed.
Po inkubaciji pri 37 °C eno uro, da pride do nevtralizacije, v vsako jamico dodamo po 0,05 ml suspenzije celic s po 0,5 do 1,0 × 106 celic na 1 ml v mediju celične kulture, ki vsebuje serum brez protiteles proti SIPV, in zapečatimo plošče.
Plates are incubated at 37 °C.
Plošče inkubiramo pri 37 °C.
Monolayers are normally confluent within 24 hours.
Enojne plasti navadno konfluirajo v 24 urah.
CPE is usually sufficiently advanced at 48 hours for a microscopic reading of the test.
Po 48 urah je CPE navadno dovolj izrazit, da je test mogoče mikroskopsko odčitati.
At this time a final microscopic reading may be made or the plates may be fixed and stained for macroscopic reading, for instance using 10 % formol-saline and 0.05 % methylene blue.
Po tem času dobimo tudi končno mikroskopsko odčitanje ali pa mikroplošče fiksiramo in obarvamo za makroskopsko odčitanje, na primer z 10-odstotno solno raztopino formola in 0,05-odstotnim metilenskim modrilom.
Controls Controls in each test include homologous antiserum of known titre, a cell control, a serum toxicity control, a medium control, and a virus titration from which the actual amount of virus in the test is calculated.
Kontrole Kontrole pri vsakem testu zajemajo homologni antiserum z znanim titrom, celično kontrolo, kontrolo toksičnosti seruma, kontrolo medija in titracijo virusa, iz katere izračunamo dejansko količino virusa pri testu.
Tests are considered to be valid when the actual amount of virus used per well in the test is between 101,5 and 102,5 TCID50 and when the titre of the reference serum is within twofold of its expected titre, estimated from the mode of previous titrations.
Testi se štejejo za veljavne, če je dejanska količina virusa, uporabljenega na jamico v testu, med 101,5 in 102,5 TCID50 in če ima titer referenčnega seruma vsaj dvakratno vrednost njegovega pričakovanega titra, ocenjeno po predhodnih titracijah.
When the controls are outside these limits the tests are repeated.
Če so kontrole zunaj teh meja, je treba test ponoviti.
An end point titre of 1/11 or less is taken as negative.
Končni titer 1:11 ali manj se šteje za negativnega.
The detection and quantification of antibody by ELISA shall be carried out according to the following protocol:
Dokazovanje in kvantificiranje protiteles s testom ELISA se izvaja v skladu z naslednjim protokolom:
Reagents Rabbit antisera to 146S antigen of seven types of foot-and-mouth disease virus (FMDV) used at a predetermined optimum concentration in carbonate/bicarbonate buffer, pH 9,6.
Reagenti Kunčji antiserumi proti antigenu 146S sedmih tipov virusa slinavke in parkljevke (SIPV), uporabljeni v vnaprej določeni optimalni koncentraciji v pufru karbonat/bikarbonat, pH 9,6.
Antigens are prepared from selected strains of virus grown on monolayers of BHK-21 cells.
Antigene pripravimo iz izbranih sevov virusa, namnoženih na enoslojnih BHK-21 celičnih linijah.
The unpurified supernatants are used and pretitrated according to the protocol but without serum, to give a dilution which after the addition of an equal volume of PBST (phosphate buffered saline containing 0,05 % Tween-20 and phenol red indicator) would give an optical density reading of between 1,2 and 1,5.
Uporabimo neprečiščene supernatante in jih predtitriramo v skladu s protokolom, vendar brez seruma, da dobimo razredčino, ki po dodatku enake količine PBST (fosfatnega pufra s soljo z 0,05 % Tween-20 in fenol rdečim indikatorjem) privede do odčitanja optične gostote med 1,2 in 1,5.
The viruses can be used inactivated.
Viruse lahko uporabimo inaktivirane.
PBST is used as a diluent.
PBST uporabimo kot razredčilo.
Guinea-pig antisera are prepared by inoculating guinea pigs with 146S antigen of each serotype.
Antiserume budre pripravimo tako, da budre (morske prašičke) inokuliramo z antigenom 146S vsakega serotipa.