natgillin/translations-raw huggingface dataset:
The competent authority may lift the control measures as regards the containment areas established in accordance with Article 2(2) and the placing on the market restrictions provided for in Article 3 after it has carried out two consecutive inspections 15 days apart that show that the increased mortality has ceased.
Pristojni organ lahko odpravi nadzorne ukrepe pri zaprtih sistemih, vzpostavljenih v skladu s členom 2(2), in omejitve pri dajanju na trg iz člena 3, potem ko je opravil dva zaporedna pregleda s 15-dnevnim presledkom, ki kažeta, da je povečani pogin prenehal.
Placing on the market requirements for Crassostrea gigas oysters originating from a compartment previously subjected to control measures due to increased mortalities in Crassostrea gigas oysters in connection with OsHV-1 μvar
Zahteve za dajanje na trg ostrig Crassostrea gigas iz kompartmenta, v katerem so se prej izvajali nadzorni ukrepi zaradi povečanega pogina ostrig Crassostrea gigas v zvezi z OsHV-1 μvar
Crassostrea gigas oysters that are placed on the market and originating from a compartment which has been subject to containment measures either in 2009 or 2010 due to increased mortalities in Crassostrea gigas oysters in connection with OsHV-1 μvar shall:
Ostrige Crassostrea gigas, ki se dajejo na trg in izvirajo iz kompartmenta, v katerem so se prej izvajali zadrževalni ukrepi leta 2009 ali leta 2010 zaradi povečanega pogina ostrig Crassostrea gigas v zvezi z OsHV-1 μvar:
be accompanied by an animal health certificate completed in accordance with the model set out in Annex II to this Regulation and the explanatory notes set out in Annex V to Regulation (EC) No 1251/2008, if the animals:
spremlja veterinarsko spričevalo, izpolnjeno po vzorcu iz Priloge II k tej uredbi in pojasnjevalnih opombah iz Priloge V k Uredbi (ES) št. 1251/2008, če so živali:
are intended for Member States or compartments which have established a programme for the early detection of OsHV-1 μvar, and in which OsHV-1 μvar is not detected; and
namenjene v države članice ali kompartmente, v katerih so vzpostavili program za zgodnje odkrivanje OsHV-1 μvar in OsHV-1 μvar ni bil odkrit, ter
are intended for farming or relaying areas;
so namenjene za območja gojenja ali za ponovno nasaditev;
originate from a compartment where the absence of OsHV-1 μVar is demonstrated by sampling and testing carried out in accordance with Part A of Annex I; and
izvirajo iz kompartmenta, za katerega je bilo z vzorčenjem in preskušanjem v skladu z delom A Priloge I dokazano, da je prost OsHV-1 μvar; in
comply with the animal health requirements set out in the model certificate, referred to in point (a).
izpolnjujejo zahteve za zdravstveno varstvo živali iz vzorca spričevala iz točke (a).
A programme for the early detection of OsHV-1 μvar referred to in paragraph 1(a)(i) shall comply with the following requirements:
Program za zgodnje odkrivanje OsHV-1 μvar iz odstavka 1(a)(i) izpolnjuje naslednje zahteve:
the programme must be declared to the Standing Committee on the Food Chain and Animal Health;
program je treba priglasiti Stalnemu odboru za prehranjevalno verigo in zdravje živali;
such declaration must be in conformity with point 1, points 5.1, 5.2, 5.3, 5.5, and 5.9, and points 6 and 7 of the model form set out in Annex II to Decision 2009/177/EC;
priglasitev mora biti v skladu s točko 1, točkami 5.1, 5.2, 5.3, 5.5 in 5.9 ter točkama 6 in 7 vzorca iz Priloge II k Odločbi 2009/177/ES;
the programme must include:
program mora vključevati:
sampling in accordance with Part A of Annex I;
vzorčenje v skladu z delom A Priloge I;
testing for the presence of OsHV-1 μvar in accordance with the diagnostic methods set out in Part B of Annex I.
preskušanje na OsHV-1 μvar z diagnostičnimi metodami iz dela B Priloge I.
Paragraph 1 shall apply one week from the date of the meeting of the Standing Committee on the Food Chain and Animal Health at which the programme referred to in paragraph 1(a)(i) was declared.
Odstavek 1 se uporablja en teden po dnevu srečanja Stalnega odbora za prehranjevalno verigo in zdravje živali, na katerem je bil priglašen program iz odstavka 1(a)(i).
Member States shall make available to the Commission and the other Member States:
Države članice Komisiji in drugim državam članicam omogočijo dostop do:
a list of containment areas and the factors that have been taken into account to define such areas, including a description of the geographical boundaries of the relevant area, established in accordance with Article 2(2);
seznama zaprtih sistemov in dejavnikov, ki so bili upoštevani pri opredelitvi takih sistemov, vključno z opisom zemljepisnih mej zadevnega območja, ki je bilo vzpostavljeno v skladu s členom 2(2);
a list of compartments including a description of the geographical boundaries of the relevant area:
seznama kompartmentov, vključno z opisom zemljepisnih mej zadevnega območja:
which have been subject to containment measures in 2009 due to increased mortalities in Crassostrea gigas oysters in connection with OsHV-1 μvar;
v katerih so se izvajali zadrževalni ukrepi leta 2009 zaradi povečanega pogina ostrig Crassostrea gigas v zvezi z OsHV-1 μvar;
where the absence of OsHV-1 μvar has been demonstrated by a testing carried out in accordance with Parts A and B of Annex I in samples taken in the containment area;
za katere je bilo s preskušanjem v skladu z deloma A in B Priloge I v vzorcih, odvzetih v zaprtem sistemu, dokazano, da so prosti OsHV-1 μvar;
declarations of programmes referred to in Article 5(2), including a description of the geographical boundaries of the relevant area.
priglasitev programov iz člena 5(2), vključno z opisom zemljepisnih mej zadevnega območja.
The information provided for in paragraph 1 shall be kept up-to-date and made available through the internet-based information pages established in accordance with Article 10 of Decision 2009/177/EC.
Informacije iz odstavka 1 se posodabljajo in so dostopne na informacijskih spletnih straneh, vzpostavljenih v skladu s členom 10 Odločbe 2009/177/ES.
By 1 October 2010 at the latest, Member States shall submit a report to the Commission on programmes declared in accordance with Article 5(2).
Najpozneje do 1. oktobra 2010 države članice predložijo Komisiji poročilo o programih, priglašenih v skladu s členom 5(2).
The report shall be in conformity with the model form set out in Annex VI to Decision 2009/177/EC.
Poročilo je v skladu z vzorčnim obrazcem iz Priloge VI k Odločbi 2009/177/ES.
It shall apply from 15 March 2010 to 31 December 2010.
Uporablja se od 15. marca 2010 do 31. decembra 2010.
Sampling for the purposes of Article 2
Vzorčenje za namene člena 2
Samples as provided for in Article 2 shall consist of at least 12 individuals of Crassostrea gigas oysters.
Vzorci iz člena 2 sestojijo iz najmanj 12 osebkov ostrig Crassotrea gigas.
When selecting those animals weak, gaping or freshly dead (not decomposed) individuals shall be sampled and they shall be collected from the compartment where the mortality is observed.
Pri izboru navedenih živali je treba vzorčiti slabotne, zevajoče ali pred kratkim poginule (nerazpadle) osebke, nabrane v kompartmentu, v katerem je bil opažen pogin.
Sampling for the purposes of Article 3(2)(b), 5(1)(b) and 5(2)
Vzorčenje za namene člena 3(2)(b) ter člena 5(1)(b) in 5(2)
Sampling for the purposes of Article 3(2)(b) shall consist of:
Vzorčenje iz člena 3(2)(b) sestoji:
in the case of larvae, five pools of at least 50 mg of whole animals collected between 4 and 8 days after fecundation including shell per consignment;
pri ličinkah: iz petih zbirov na pošiljko po najmanj 50 mg celih živali, nabranih med 4 in 8 dnevi po oploditvi, vključno z lupino;
in the case of spat smaller than 6 mm, 30 pools of 300 mg of whole animals including shell per consignment;
pri mladicah, manjših od 6 mm: iz 30 zbirov na pošiljko po 300 mg celih živali, vključno z lupino;
in the case of oysters bigger than 6 mm, 150 individuals per consignment.
pri ostrigah, večjih od 6 mm: iz 150 osebkov na pošiljko.
When selecting those animals, all parts of the consignment must be proportionally represented in the sample.
Pri izboru navedenih živali je treba v vzorcu sorazmerno upoštevati vse dele pošiljke.
If weak, gaping or freshly dead (not decomposed) animals are present, primarily such animals shall be selected.
Če so navzoče slabotne, zevajoče ali pred kratkim poginule (nerazpadle) živali, se najprej izberejo take živali.
Sampling for the purposes of Article 5(2) shall consist of at least 150 individuals of Crassostrea gigas per sampling points.
Vzorčenje iz člena 5(2) sestoji iz najmanj 150 osebkov ostrige Crassotrea gigas na točko odvzema vzorcev.
All farms or mollusc farming areas in the Member State or compartment covered by the programme shall be sampled.
Vzorčijo se vse ribogojnice ali območja gojenja mehkužcev v državah članicah ali kompartmentih, ki jih zajema program.
Sampling for the purposes of Article 5(1)(b) shall consist of at least 150 individuals of Crassostrea gigas oysters per compartment.
Vzorčenje iz člena 5(1)(b) sestoji iz najmanj 150 osebkov ostrige Crassotrea gigas na kompartment.
When selecting those animals, the following criteria shall be taken into account:
Pri izboru navedenih živali je treba upoštevati naslednja merila:
If such animals are not present, the animals selected shall include healthy molluscs less than 12 months old.
Če takih živali ni, izbrane živali vključujejo zdrave mehkužce, mlajše od 12 mesecev;
When sampling in farms in which more than one water source is utilised for production, animals representing all water sources must be included for sampling in such a way that all parts of the farm are proportionally represented in the sample.
pri vzorčenju v ribogojnicah, v katerih se za proizvodnjo uporablja več kot en vodni vir, je treba živali, ki predstavljajo vse vodne vire, vključiti za vzorčenje tako, da so vsi deli ribogojnice sorazmerno upoštevani v vzorcu;
When sampling in mollusc farming areas, animals from a sufficient number of sampling points, at least three sampling points, shall be included in the sample in such a way that all parts of the mollusc farming area are proportionally represented in the sample, including natural beds present in the mollusc farming area.
pri vzorčenju na območjih gojenja mehkužcev je treba živali iz zadostnega števila (najmanj treh) točk odvzema vključiti v vzorec tako, da so vsi deli območja gojenja mehkužcev sorazmerno upoštevani v vzorcu, vključno z naravnimi školjčišči na območju gojenja mehkužcev.
The main factors to be considered for the selection of these sampling points are: previous detection of OsHV-1 μvar in the area, stocking density, water flows, bathymetry and management practices.
Glavni dejavniki, ki jih je treba upoštevati za izbor teh točk odvzema, so: predhodno odkritje OsHV-1 μvar na območju, gostota živali, vodni tokovi, batimetrija in upravljavskih praks.
The sampling provided for in Article 5(2) shall be carried out in the period of the year when prevalence of OsHV-1 μvar in the Member State or compartment is known to be maximal.
Vzorčenje iz člena 5(2) je treba opravi v obdobju leta, za katerega je znano, da je razširjenost OsHV-1 μvar v državi članici ali kompartmentu največja.
When such data is not available, sampling shall be carried out just after the period when the water temperature exceeds 16 °C or at the time of the year when the temperature normally reaches its yearly maximum.
Ko tak podatek ni na voljo, se vzorčenje opravi tik po obdobju, ko temperatura vode preseže 16 °C, ali v obdobju leta, ko temperatura običajno doseže letni višek.
The sampling provided for in Article 5(1)(b) shall preferably be carried out in the period of the year described in point c. If samples are collected outside that period of the year, the sampled oysters must be maintained under conditions equivalent to those described in point c for a period suitable for the detection of OsHV-1 μvar, before being tested.
Vzorčenje iz člena 5(1)(b) se najraje opravi v obdobju leta iz točke (c). Če se vzorci nabirajo zunaj navedenega obdobja leta, je treba vzorčene ostrige pred preskušanjem vzdrževati pod pogoji, enakovrednim pogojem iz točke (c), in sicer za čas, ki je primeren za odkritje OsHV-1 μ var.
Diagnostic methods of detecting OsHV-1 μvar
Diagnostične metode za odkrivanje OsHV-1 μ var
This procedure explains a standard diagnostic method to be used for OsHV-1 μvar detection and identification by Polymerase Chain Reaction (hereinafter PCR).
Ta postopek razlaga standardno diagnostično metodo, ki se uporablja za odkrivanje OsHV-1 μ var in identifikacijo z verižno reakcijo s polimerazo (v nadaljnjem besedilu: PCR).
It allows distinguishing between OsHV-1 and OsHV-1 μvar.
Metoda omogoča razločevanje OsHV-1 od OsHV-1 μ var.
When appropriate, in order to optimise the reaction conditions and to suit the equipment and conditions in their own laboratory, the laboratories may apply modifications to the methods described in this Annex, provided that an equal sensitivity and specificity can be demonstrated.
Po potrebi lahko laboratoriji spremenijo metode iz te priloge za optimizacijo pogojev za reakcijo ter prilagajanje laboratorijski opremi in razmeram, če je mogoče dokazati enako občutljivost in specifičnost.
OsHV-1 μvar is defined in Article 1 of this Regulation.
OsHV-1 μ var je opredeljen v členu 1 te uredbe.
Equipment and environmental conditions
Oprema in razmere okolja
The diagnostic test used for OsHV-1 μvar detection and identification by PCR requires the equipment and environmental conditions classically used for PCR assays as follows:
Diagnostični preskus, ki se uporablja za odkrivanje OsHV-1 μ var in identifikacijo s PCR, zahteva, da so oprema in razmere okolja, ki se klasično uporabljajo za teste PCR, naslednje:
A closed hood equipped with an UV producing system to eliminate potential contaminations when preparing PCR mix.
zaprt pokrov, opremljen s sistemom za UV sevanje za preprečitev morebitne kontaminacije pri pripravi mešanice za PCR,
Two complete sets of pipettes (2 μl; 20 μl; 200 μl and 1000 μl), the first one for DNA extraction, and the second one for PCR mix preparation.
dve popolni seriji pipet (2 μl; 20 μl; 200 μl in 1000 μl), prva za ekstrakcijo DNK, druga za pripravo mešanice za PCR,
Three different pipettes: one pipette (2 μl) to dispense samples in PCR mix, one pipette (20 μl) for EB sampling and another pipette (20 μl) to load PCR products in agarose gels.
tri različne pipete: ena pipeta (2 μl) za pipetiranje vzorcev v mešanico za PCR, ena pipeta (20 μl) za vzorčenje EB in druga pipeta (20 μl) za nanašanje produktov PCR v agarozne gele,
Filter pipette tips (2 μl; 20 μl; 200 μl and 1000 μl) for DNA extraction, PCR mix preparation and sample dispensing.
pipetne konice s filtrom (2 μl; 20 μl; 200 μl in 1000 μl) za ekstrakcijo DNK, pripravo mešanice za PCR in pipetiranje vzorcev,
Pipette tips (20 μl) to collect EB and to load amplification products in agarose gel.
pipetne konice (20 μl) za zbiranje EB in nanašanje pomnoževalnih produktov v agarozni gel,
A thermal cycler to perform amplifications.
ciklični termostat za izvajanje pomnoževanja,
A horizontal electrophoresis system for PCR products electrophoresis.
vodoravni elektroforezni sistem za elektroforezo produktov PCR,
An UV table to observe PCR products after agarose gel electrophoresis.
UV transiluminator za opazovanje produktov PCR po agarozni gelski elektroforezi,
A system to acquire pictures of the gels.
sistem za slikanje gelov.
The manipulator must wear a lab coat and some gloves during all the different steps described bellow.
Izvajalec mora nositi zaščitno haljo in rokavice pri vseh različnih korakih, ki so opisani v nadaljevanju.
Lab coat and gloves must be changed preferably after each main step: DNA extraction, preparation of PCR mix, sample dispensing, amplification and gel loading.
Po možnosti je treba zamenjati zaščitno haljo in rokavice po vsakem večjem koraku: ekstrakciji DNK, pripravi mešanice za PCR, pipetiranju vzorcev, pomnoževanju in nanašanju v gel.
It is recommended to perform these different steps in different rooms.
Priporoča se, da se ti različni koraki opravijo v različnih prostorih.
More particularly, amplification and gel loading/electrophoresis should take place in a room separate from DNA extraction, PCR mix preparation and DNA dispensing.
Natančneje, pomnoževanje in nanašanje v gel/elektroforeza morata potekati v prostoru, ki je ločen od ekstrakcije DNK, priprave mešanice za PCR in dodajanja DNK.
Live or freshly dead (not decomposed) oysters, which can be previously frozen, are processed for DNA extraction.
Žive ali pred kratkim poginule (nerazpadle) ostrige, ki jih je mogoče prej zamrzniti, se pripravijo za ekstrakcijo DNK.
Samples are processed differently according to their size:
Vzorci se glede na velikost pripravijo različno:
For larvae, pools of 50 mg of the whole animals (including the shell) completed with 200 μl of distilled water are crushed and centrifuged at 1000 g for 1 minute.
za ličinke: se zbiri po 50 mg celih živali (vključno z lupino), ki se jim doda 200 μl destilirane vode, strejo in 1 minuto centrifugirajo pri 1000 g;
For spat smaller than or of 6 mm, pools of 300 mg of the whole animals (including the shell) completed with 1200 μl of distilled water are crushed and centrifuged at 1000 g for 1 minute.
za mladice, manjše od 6 mm: se zbiri po 300 mg celih živali (vključno z lupino), ki se jim doda 1200 μl destilirane vode, strejo in 1 minuto centrifugirajo pri 1000 g;
For spat between 6 and 15 mm in size, all the soft tissues of each animal are crushed individually.
za mladice, velike med 6 in 15 mm: se vsa mehka tkiva vsake živali strejo posamezno;
For animals bigger than 15 mm, pieces of gills and mantle are isolated.
za živali, večje od 15 mm: se izolirajo deli škrg in plašča.
DNA extraction is performed using the QIAamp® DNA Mini Kit (QIAGEN) and following the instructions for Tissue Test Protocol.
Ekstrakcija DNK se opravi z uporabo QIAamp® DNA Mini Kita (QIAGEN) in upoštevanjem navodil iz postopka za preskušanje tkiva.
The further sample preparation is performed in the following order:
Nadaljnja priprava vzorca poteka v naslednjem vrstem redu:
Place 100 μl of supernatant for samples referred to in (a) and (b) or 10 to 50 mg of tissues for samples referred to point (c) and (d) in a 1,5 ml microcentrifuge tube and add 180 μl of Buffer ATL.
Položimo 100 μl supernatanta za vzorce iz točk (a) in (b) ali 10 do 50 mg tkiv za vzorce iz točk (c) in (d) v 1,5-mililitrsko epruvetko za mikrocentrifugiranje ter dodamo 180 μl pufra ATL.
Add 20 μl Proteinase K, mix by vortexing and incubate at 56 °C until the tissue is completely lysed (overnight).
Dodamo 20 μl proteinaze K, zmešamo z vrtinčenjem in inkubiramo pri 56 °C, dokler tkivo ni popolnoma lizirano (čez noč).
Vortex occasionally during incubation to disperse sample.
Občasno vrtinčimo med inkubacijo, da se vzorec razprši.
Briefly centrifuge the 1,5 ml microcentrifuge tube to remove drops from the lid.
Na kratko centrifugiramo 1,5-mililitrsko epruvetko za mikrocentrifugiranje, da odstranimo kapljice s pokrovčka.
Add 200 μl Buffer AL to the sample, mix by pulse-vortexing for 15 s and incubate at 70 °C for 10 minutes.
Vzorcu dodamo 200 μl pufra AL, mešamo 15 sekund s pulznim vrtinčenjem in 10 minut inkubiramo pri 70 °C.
Add 200 μl ethanol (96-100 %) to the sample, and mix by pulse-vortexing for 15 s. Briefly centrifuge the 1,5 ml microcentrifuge tube to remove drops from the lid.
Vzorcu dodamo 200 μl etanola (96–100 %) in mešamo 15 sekund s pulznim vrtinčenjem. Na kratko centrifugiramo 1,5-mililitrsko epruvetko za mikrocentrifugiranje, da odstranimo kapljice s pokrovčka.
Carefully apply the mixture from step 4 to the QIAamp Spin Column (in a 2 ml collection tube) without wetting the rim.
Previdno prelijemo mešanico iz koraka 4 v kolono QIAamp Spin Column (v 2-mililitrsko zbiralno epruvetko), ne da bi zmočili rob.
Close the cap and centrifuge at 10000 rpm for 1 min. Place the QIAamp Spin Column in a clean 2 ml collection tube (provided in the kit) and discard the tube containing the filtrate.
Zapremo pokrovček in centrifugiramo 1 minuto pri 10000 obratih na minuto. Položimo kolono QIAamp Spin Column v čisto 2-mililitrsko zbiralno epruvetko (priloženo v kitu) in zavržemo epruvetko, ki vsebuje filtrat.
Carefully open the QIAamp Spin Column and add 500 μl Buffer AW1 without wetting the rim.
Previdno odpremo kolono QIAamp Spin Column in dodamo 500 μl pufra AW1, ne da bi zmočili rob.
Close the cap and centrifuge at 10000 rpm for 1 min. Place the QIAamp Spin Column in a clean 2 ml collection tube (provided in the kit) and discard the collection tube containing the filtrate.
Zapremo pokrovček in centrifugiramo 1 minuto pri 10000 obratih na minuto. Položimo kolono QIAamp Spin Column v čisto 2-mililitrsko zbiralno epruvetko (priloženo v kitu) in zavržemo zbiralno epruvetko, ki vsebuje filtrat.
Carefully open the QIAamp Spin Column and add 500 μl Buffer AW2 without wetting the rim.
Previdno odpremo kolono QIAamp Spin Column in dodamo 500 μl pufra AW2, ne da bi zmočili rob.
Close the cap and centrifuge at full speed (14000 rpm) for 3 min.
Zapremo pokrovček in centrifugiramo 3 minute pri polni hitrosti (14000 obratov na minuto).
(Optional) Place the QIAamp Spin Column in a new 2ml collection tube (not provided in the kit) and discard the collection tube containing the filtrate.
(Neobvezno) položimo kolono QIAamp Spin Column v novo 2-mililitrsko zbiralno epruvetko (ki ni priložena v kitu) in zavržemo zbiralno epruvetko, ki vsebuje filtrat.
Centrifuge at full speed (14000 rpm) for 1 min.
Centrifugiramo 1 minuto pri polni hitrosti (14000 obratov na minuto).
Place the QIAamp Spin Column in a clean 1,5 ml microcentrifuge tube (not provided in the kit) and discard the collection tube containing the filtrate.
Položimo kolono QIAamp Spin Column v čisto 1,5-mililitrsko epruvetko za mikrocentrifugiranje (ki ni priložena v kitu) in zavržemo zbiralno epruvetko, ki vsebuje filtrat.
Carefully open the QIAamp Spin Column and add 100 μl of distilled water.
Previdno odpremo kolono QIAamp Spin Column in dodamo 100 μl destilirane vode.
Incubate 5 minutes at room temperature and centrifuge at 10000 rpm for 1 min.
Inkubiramo 5 minut na sobni temperaturi in centrifugiramo 1 minuto pri 10000 obratih na minuto.
Control the quality and efficacy of the extraction (for example by measuring OD (260 nm) under spectrophotometer or after electrophoresis in agarose gel).
Pregledamo kakovost in učinkovitost ekstrakcije (na primer z merjenjem vsebnosti raztopljenega kisika (260 nm) pod spektrofotometrom ali po agarozni gelski elektroforezi).
Prepare dilution of your samples in order to have a final DNA concentration of 50-100 ng/μl.
Pripravimo razredčino vzorcev, da je končna koncentracija DNK 50–100 ng/μl.
DNA solutions are kept at 4 °C until PCR analyses are performed.
Raztopine DNK se hranijo pri 4 °C do opravljene analize PCR.
Other commercial kits may be used for the DNA extractions provided they have been demonstrated to give similar results.
Za ekstrakcijo DNK se lahko uporabljajo drugi komercialni kiti, če je dokazano, da dajo podobne rezultate.
Polymerase Chain Reaction (PCR)
Verižna reakcija s polimerazo (PCR)
Reactives
Reagenti
10 X Buffer (furnished with the Taq DNA polymerase)
10 X pufer (priložen s Taq DNA polimerazo)
MgCl2 (furnished with the DNA polymerase) (25 mM)
MgCl2 (priložen z DNA polimerazo) (25 mM)
Taq DNA Polymerase (Goldstar, Eurogentec) 5 U/μl
Taq DNA polimeraza (Goldstar, Eurogentec) 5 U/μl
dNTP (dATP, dCTP, dGTP, dTTT) Master Mix (20mM) must be diluted 10 fold (at 2 mM) before use
dNTP (dATP, dCTP, dGTP, dTTT) Master Mix (20 mM), pred uporabo je treba 10-kratno razredčiti (pri 2 mM)
d H2O (distilled H2O free of DNA and RNA)
d H2O (destilirana H2O brez DNK in RNK)
The following primers [1] must be used:
Uporabiti je treba naslednje temeljne premaze [1]:
PCR mix
Mešanica za PCR
PCR mix for each tube is:
Mešanica za PCR za vsako epruvetko je:
Volume per tube
Prostornina epruvete
Buffer (10 X)
pufer (10 X)
Taq polymérase (5U/μl)
Taq polimeraza (5 U/μl)
49 μl of this PCR mix is dispensed in each PCR tube
49 μl te mešanice za PCR se da v vsako epruvetko za PCR,
1 μl of extracted DNA (50-100 ng/μl) is added to each tube
1 μl ekstrahirane DNK (50–100 ng/μl) se doda v vsako epruvetko.