natgillin/translations-raw huggingface dataset:
A predetermined optimum concentration is prepared in PBST containing 10 % normal bovine serum and 5 % normal rabbit serum.
Vnaprej določeno optimalno koncentracijo pripravimo v PBST, ki vsebuje 10 % normalni bovini serum in 5 % normalni kunčji serum.
Rabbit anti-guinea-pig immunoglobulin conjugated to horseradish peroxidase is used at a predetermined optimum concentration in PBST containing 10 % normal bovine serum and 5 % normal rabbit serum.
Kunčji protibudrin imunoglobulin, konjugiran na hrenovo peroksidazo (HRP), uporabimo v vnaprej določeni optimalni koncentraciji v PBST, ki vsebuje 10 % normalnega bovinega seruma in 5 % normalnega kunčjega seruma.
Test sera are diluted in PBST.
Preiskovane serume razredčimo v PBST.
ELISA plates are coated with 50 μl of rabbit antiviral sera overnight in a humidity chamber at room temperature.
Mikroplošče ELISA prekrijemo s 50 μl kunčjih antiserumov in jih pustimo stati čez noč v vlažni komori pri sobni temperaturi.
Fifty microlitres of a duplicate, twofold series of each test serum starting at 1/4 are prepared in U-bottomed multiwell plates (carrier plates).
Petdeset mikrolitrov v dveh ponovitvah dvakratnih razredčin vsakega testnega seruma z začetno razredčitvijo 1:4 pripravimo na večjamičnih mikroploščah z dnom v obliki črke U (nosilnih ploščah).
Fifty microlitres of a constant dose of antigen are added to each well and the mixtures are left overnight at 4 °C.
Petdeset mikrolitrov konstantnega odmerka antigena dodamo v vsako jamico in mešanice pustimo stati čez noč pri 4 °C.
The addition of the antigen reduces the starting serum dilution to 1/8.
Z dodatkom antigena razredčimo začetno razredčino seruma na 1:8.
The ELISA plates are washed five times with PBST.
Mikroplošče ELISA petkrat speremo s PBST.
Fifty microlitres of serum/antigen mixtures are then transferred from the carrier plates to the rabbit-serum-coated ELISA plates and incubated at 37 °C for one hour on a rotary shaker.
Petdeset mikrolitrov mešanic serum/antigen prenesemo z nosilnih plošč na mikroplošče ELISA, prekrite s kunčjim serumom, in jih inkubiramo pri 37 °C eno uro na orbitalnem stresalniku.
After washing, 50 μl of guinea-pig antiserum to the antigen used in point 4 is added to each well.
Po spiranju dodamo v vsako jamico po 50 μl budrinega antiseruma proti antigenu v skladu s točko 4.
The plates are incubated at 37 °C for one hour a rotary shaker.
Mikroplošče inkubiramo pri 37 °C eno uro na orbitalnem stresalniku.
The plates are washed and 50 μl of rabbit anti-guinea-pig immunoglobulin conjugated to horseradish peroxidase is added to each well.
Mikroplošče speremo in v vsako jamico dodamo po 50 μl kunčjega antibudrinega imunoglobulina, konjugiranega na hrenovo peroksidazo (HRP).
The plates are incubated at 37 °C for one hour on a rotary shaker.
Mikroplošče inkubiramo pri 37 °C eno uro na orbitalnem stresalniku.
The plates are washed and 50 μl of orthophenylene diamine containing 0,05 % H2O2 (30 %) w/v is added to each well.
Mikroplošče speremo in v vsako jamico dodamo po 50 μl ortofenilen-diamina z 0,05 % H2O2 (30 %) vol:vol.
The reaction is stopped after 15 minutes with 1,25M H2SO4.
Reakcijo ustavimo po 15 minutah z 1,25M H2SO4.
The plates are read spectrophotometrically at 492 nm on an ELISA reader linked to a microcomputer.
Mikroplošče odčitamo spektrofotometrično pri 492 nm na čitalniku testa ELISA, povezanem z mikroračunalnikom.
Controls For each antigen used 40 wells contain no serum but contain antigen diluted in PBST.
Kontrole Za vsak uporabljeni antigen 40 jamic brez seruma, ki vsebujejo antigen, razredčen v PBST.
A duplicated twofold dilution series of homologous bovine reference antiserum.
Dvakratne razredčine v dveh ponovitvah homolognega bovinega referenčnega antiseruma.
A duplicate twofold dilution series of negative bovine serum.
Dvakratne razredčine v dveh ponovitvah govejega seruma.
Interpretation Antibody titres are expressed as the final dilution of tests serum giving 50 % of the mean OD value recorded in the virus control wells where test serum is absent.
Razlaga Titri protiteles so izraženi kot končna razredčitev testnih serumov, ki dosežejo 50 % srednje vrednosti OD, ugotovljene v jamicah s kontrolami virusa brez testnega seruma.
Titres in excess of 1/40 are considered positive.
Vrednosti titrov nad 1:40 se štejejo za pozitivne.
References Hamblin C, Barnett ITR and Hedger RS (1986) ‘A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus.
Sklici Hamblin C, Barnett ITR in Hedger RS (1986) „A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus.
I. Development and method of ELISA.’ Journal of Immunological Methods, 93, 115 to 121.11.
I. Development and method of ELISA.“ Journal of Immunological Methods, 93, 115 do 121.11.
Aujeszky's disease (AJD)
Bolezen Aujeszkega (BA)
Procedure The constant virus-varying serum neutralisation test on microtitre plates employs Vero or other sensitive cell systems.
Postopek Za serum-nevtralizacijski test na mikroploščah, pri katerem se virus konstantno spreminja, uporabimo celično linijo Vero ali druge dovzetne celice.
Aujeszky's disease virus is used at 100 TCID50 per 0,025 ml; inactivated undiluted serum samples are mixed with an equal volume (0,025 ml) of virus suspension.
Virus bolezni Aujeszkega uporabimo pri 100 TCID50 na 0,025 ml; inaktivirane nerazredčene vzorce seruma zmešamo z enako količino (0,025 ml) suspenzije virusa.
The virus/serum mixtures are incubated for two hours at 37 °C in the microtitre plates before the appropriate cells are added.
Mešanice virus/serum inkubiramo dve uri pri 37 °C na mikroploščah, preden jim dodamo ustrezne celice.
Interpretation The results of the neutralisation test and the titre of the virus used in the test are recorded after three to seven days incubation at 37 °C.
Razlaga Rezultate serum-nevtralizacijskega testa in titer virusa, uporabljenega v testu, zabeležimo po treh do sedmih dneh inkubacije pri 37 °C.
Serum titres less than 1/2 (undiluted serum) are considered negative.
Titri seruma, manjši od 1:2 (nerazredčeni serum), se štejejo za negativne.
Any other test recognised in the framework of Decision 2008/185/EC [11].Transmissible gastro-enteritis (TGE)
Kateri koli drug test v okviru Odločbe 2008/185/ES [11].Transmisivni gastroenteritis (TGE)
Procedure The constant virus-varying serum neutralisation test on microtitre plates employs A72 (dog tumour) cells or other sensitive cell systems.
Postopek Za serum-nevtralizacijski test na mikroploščah, pri katerem se virus konstantno spreminja, uporabimo celično linijo A72 (celice pasjega tumorja) ali druge dovzetne celice.
TGE virus is used at 100 TCID50 per 0,025 ml; inactivated undiluted serum samples are mixed with an equal volume (0,025 ml) of virus suspension.
Virus TGE uporabimo pri 100 TCID50 na 0,025 ml; inaktivirane nerazredčene vzorce seruma zmešamo z enako količino (0,025 ml) suspenzije virusa.
The virus/serum mixtures are incubated for 30 to 60 minutes at 37 °C in the microtitre plates before the appropriate cells are added.
Mešanice virus/serum inkubiramo 30 do 60 minut pri 37 °C na mikroploščah, preden jim dodamo ustrezne celice.
Each cell receives 0,1 ml of cell suspension.
Vsaka celica sprejme 0,1 ml celične suspenzije.
Interpretation The results of the neutralisation test and the titre of the virus used in the test are recorded after three to five days incubation at 37 °C.
Razlaga Rezultate serum-nevtralizacijskega testa in titer virusa, uporabljenega v testu, zabeležimo po treh do petih dneh inkubacije pri 37 °C.
Serum titres less than 1/2 (final dilution) are considered negative.
Titri seruma, manjši od 1:2 (končna razredčitev), se štejejo za negativne.
Serum titres of less than 1/4 (final dilution) are considered negative in these cases.
V teh primerih se titri seruma, manjši od 1:4 (končna razredčitev), štejejo za negativne.
Swine vesicular disease (SVD)
Vezikularna bolezen prašičev (SVD)
Tests for swine vesicular disease (SVD) shall be carried out according to Decision 2000/428/EC [12].Classical swine fever (CSF)
Preskusi za vezikularno bolezen prašičev (SVD) se izvajajo v skladu z Odločbo 2000/428/ES [12].Klasična prašičja kuga (KPK)
Tests for classical swine fever (CSF) shall be carried out according to Decision 2002/106/EC [13].The performance of tests for CSF must follow the guidelines set out in the relevant chapter of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
Preskusi za klasično prašičjo kugo (KPK) se izvajajo v skladu z Odločbo 2002/106/ES [13].Preskusi za KPK morajo potekati v skladu s smernicami iz ustreznega poglavja Poslovnika standardnih postopkov za diagnostične preskuse in cepiva za kopenske živali – OIE.
The evaluation of sensitivity and specificity of the serological test for CSF must be carried out in a national laboratory with a quality assurance scheme in place.
Oceno občutljivosti in specifičnosti serološkega testa za KPK se mora določiti v nacionalnem laboratoriju z uvedenim sistemom zagotavljanja kakovosti.
Tests employed must be shown to recognise a range of weak and strong positive reference sera and allow detection of antibody in early phase and convalescence.
Uporabljeni testi morajo biti dokazano sposobni prepoznavati razpone šibko in močno pozitivnih referenčnih serumov ter omogočati dokazovanje protiteles v zgodnji fazi bolezni in fazi rekonvalescence.
Import and quarantine animal health conditions for animals imported into St. Pierre and Miquelon within a period of less than six months prior to introduction into the Union
Pogoji zdravstvenega varstva za uvoz in karanteno za živali, uvožene v St. Pierre in Miquelon v obdobju, krajšem od šest mesecev pred vnosom v Unijo
Animal species covered
Zajete živalske vrste
ORDER
RED
FAMILY
DRUŽINA
GENUS AND SPECIES
ROD IN VRSTA
Residence and quarantine
Bivanje in karantena
Animals imported into St. Pierre and Miquelon must reside in an authorised quarantine station for a minimum period of 60 days before being dispatched for introduction into the Union.
Živali, uvožene v St. Pierre in Miquelon, morajo bivati v odobreni karantenski postaji najmanj 60 dni pred odpremo za vnos v Unijo.
This period may be increased due to testing requirements for individual species.
To obdobje se lahko podaljša glede na zahteve testov za posamezne vrste.
In addition the animals must comply with the following requirements:
Poleg tega morajo živali izpolnjevati naslednje zahteve:
Separate consignments may enter the quarantine station.
v karantensko postajo lahko pridejo različne pošiljke živali.
However, upon entry in the quarantine station all animals of the same species in the quarantine facility must be considered as a single group, and referred to as such.
Vendar je treba po prihodu v karantensko postajo vse živali iste vrste šteti za eno skupino in jih tako tudi obravnavati.
The quarantine period must commence for the whole group at the time when the last animal entered the quarantine facility.
V tem primeru se začne obdobje karantene za celotno skupino na dan, ko je zadnja žival prišla v karanteno;
Within the quarantine station each specific group of animals must be maintained in isolation, with no direct or indirect contact with any other animals, including those from other consignments that may be present.
v karantenski postaji je treba posamezne skupine živali ločiti tako, da ne pridejo neposredno ali posredno v stik z drugimi živalmi, vključno z morebitno prisotnimi živalmi iz drugih pošiljk.
Each consignment must be kept in the approved quarantine station and protected from vector insects.
Posamezne pošiljke živali morajo biti nastanjene v odobreni karantenski postaji in zaščitene pred vektorskimi insekti;
If, during the period of quarantine, the isolation of a group of animals is not maintained and contact is made with other animals, the quarantine period must begin again for the same duration as initially prescribed on entry into the quarantine station.
če se v času karantene ne upošteva izolacija skupine živali in te pridejo v stik z drugimi živalmi, se mora začeti novo obdobje karantene v enakem trajanju, kakor je bilo prvotno predpisano pri vstopu v karantensko postajo;
Animals to be introduced into the Union which pass through the quarantine station must be loaded and dispatched directly to the Union:
živali, namenjene za vnos v Unijo, ki gredo skozi karantensko postajo, morajo biti natovorjene in odpremljene neposredno v Unijo:
without coming into contact with animals other than animals which fulfil the health conditions established for the introduction of the relevant category of animal into the Union;
ne da bi prišle v stik z živalmi, ki ne izpolnjujejo pogojev glede zdravstvenega stanja živali, predpisanih za vnos zadevne kategorije živali v Unijo;
segregated into consignments so that no consignment can came in contact with animals not eligible for importation into the Union;
razdeljene na pošiljke tako, da nobena pošiljka ne more priti v stik z živalmi, ki ne izpolnjujejo pogojev za uvoz v Unijo;
in transport vehicles or containers which have first been cleansed and disinfected with a disinfectant officially authorised in St. Pierre and Miquelon as effective in the control of the diseases referred to in Chapter 2 and which are so constructed that faeces, urine, litter or fodder cannot flow or fall out of the vehicle or container during transportation.
v prevoznih sredstvih ali zabojnikih, prej očiščenih in razkuženih z razkužilom, ki je v St. Pierru in Miquelonu uradno odobreno kot učinkovito pri obvladovanju bolezni iz poglavja 2, in zgrajenih tako, da iztrebki, urin, stelja ali krma med prevozom ne morejo iztekati ali izpadati iz vozila ali zabojnika.
The quarantine premises must at least meet the minimum standards laid down in Annex B to Directive 91/496/EEC [14], and the following conditions:(a) they must be supervised by an official veterinarian;
Prostori karantene morajo ustrezati vsaj minimalnim standardom iz Priloge B k Direktivi 91/496/EGS [14] in izpolnjevati naslednje pogoje:(a) morajo biti pod nadzorom uradnega veterinarja;
they must be situated at the centre of an area of at least 20 km in diameter in which, according to official findings, for at least 30 days prior to their use as a quarantine station there has been no case of foot-and-mouth disease;
morajo ležati sredi območja s premerom vsaj 20 km, na katerem po uradnih ugotovitvah vsaj 30 dni pred uporabo kot odobrena karantenska postaja ni bilo nobenega primera slinavke in parkljevke;
they must, before being used as a quarantine station, be cleansed and disinfected with a disinfectant officially authorised in St Pierre et Miquelon as effective in the control of the diseases referred to in Chapter 2;
pred uporabo kot karantenska postaja morajo biti očiščeni in razkuženi z razkužilom, ki ga St. Pierre in Miquelon uradno prizna za učinkovitega pri obvladovanju bolezni iz poglavja 2;
they must operate, taking into account their animal capacity:
ob upoštevanju zmogljivosti glede števila živali morajo zajemati:
a facility dedicated exclusively for the quarantine of animals, including adequate housing to a suitable standard for the animals;
prostor, namenjen izključno za karanteno živali, vključno s primernim bivališčem ustreznega standarda za živali;
appropriate facilities, that:
primerne prostore, ki:
are easy to thouroughly clean and disinfect,
so enostavni za temeljito čiščenje in razkuževanje,
include facilities for safe loading and unloading,
vsebujejo prostore za varno natovarjanje in raztovarjanje,
are able to fulfil all watering and feeding requirements for the animals,
izpolnjujejo vse zahteve glede napajanja in hranjenja živali,
allow any necessary veterinary treatment to be easily administered;
omogočajo enostavno opravljanje nujnega veterinarskega zdravljenja;
appropriate equipment for cleaning and disinfecting rooms and transport vehicles;
primerno opremo za čiščenje in razkuževanje prostorov in prevoznih sredstev;
an appropriate system for collecting waste water;
primeren sistem za zbiranje odpadne vode;
an office for the official veterinarian;
pisarno za uradnega veterinarja;
when operating, they must have sufficient veterinarians to carry out all duties;
kadar obratujejo, mora biti na voljo dovolj veterinarjev za izvajanje vseh nalog;
they must only admit animals that are individually identified so as to guarantee traceability.
sprejemati smejo le posamezno označene živali, da je zagotovljena sledljivost.
To this end, when animals are admitted the owner or the person in charge of the quarantine station must ensure that the animals are properly identified and accompanied by health certificates for the species and categories involved.
V ta namen mora lastnik karantenske postaje ali oseba, odgovorna za karantensko postajo, ob sprejemanju živali zagotoviti, da so pravilno označene in jih spremljajo spričevala o zdravstvenem stanju za zadevne živalske vrste ali kategorije.
In addition, the owner or the person in charge of the quarantine station must record on a register or in a data base, and retain for at least three years, the name of the owner, the origin of the animals in the consignment, the dates of entry and exit of the animals in the consignment, the identification number of the animals in the consignment and their place of destination;
Poleg tega mora lastnik karantenske postaje ali oseba, odgovorna za karantensko postajo, v registru ali podatkovni bazi evidentirati in vsaj tri leta hraniti ime lastnika, izvor živali v pošiljki, datum vstopa in izstopa živali iz pošiljke, identifikacijsko številko živali v pošiljki in njihov namembni kraj;
the competent authority must determine the procedure for official supervision of the quarantine station and must ensure that such supervision is carried out; this supervision must include regular inspections in order to ascertain that the requirements for approval continue to be fulfilled.
pristojni organ mora določiti postopek uradnega nadzora karantenske postaje in zagotoviti, da se tak nadzor izvaja; nadzor mora vključevati redne preglede, da se preveri, ali so zahteve za odobritev še izpolnjene.
In case of failure and suspension, the approval may only be restored when the competent authority is satisfied that the quarantine premises are in full compliance with all the conditions set out in points (a) to (g).
V primeru neizpolnjevanja zahtev in začasnega preklica odobritve lahko pristojni organ prostore karantene ponovno odobri šele, ko se prepriča, da v celoti izpolnjuje vse pogoje iz točk (a) do (g).
Animal health tests
Testi zdravstvenega stanja živali
The animals must be subjected to the following tests carried out on samples of blood taken, if not specified otherwise, not earlier than 21 days from the date of commencement of the isolation period.
Pri živalih je treba opraviti naslednje teste, ki se izvajajo na vzorcih krvi, odvzetih ne prej kot 21 dni po začetku obdobja izolacije, če ni določeno drugače.
The laboratory tests must be carried out in an approved laboratory in the Union and all laboratory tests and their results, vaccinations and treatments must be enclosed with the health certificate.
Laboratorijski testi morajo biti opravljeni v odobrenem laboratoriju v Uniji ter vsi laboratorijski testi in njihovi rezultati, cepljenja in zdravljenja morajo biti priloženi veterinarskemu spričevalu
In order to keep animal interventions to a minimum, sampling, tests and any vaccinations must be grouped as far as is possible whilst respecting the minimum time intervals required by the testing protocols set out in Part 2 of this Chapter.
Da se posegi na živalih ohranijo na kar najnižji ravni, se mora odvzem vzorcev, testi in kakršna koli cepljenja združiti kolikor najbolj mogoče ob upoštevanju najmanjših časovnih intervalov, ki se zahtevajo za protokole testiranja v delu 2 tega poglavja.
CAMELIDAE
DRUŽINA KAMEL (CAMELIDAE)
Test to be used: comparative intradermal reaction test using Bovine purified protein derivative (PPD) and Avian PPD conforming to the standards for the manufacture of bovine and avian tuberculins as described in point 2.1.2 of Annex B of Directive 64/432/EEC.
Uporabi se test: intradermalni primerjalni preskus z bovinim prečiščenim proteinskim derivatom (PPD) in aviarnim PPD, ki ustrezajo standardom za proizvodnjo bovinih in aviarnih tuberkulinov, kot je opisano v točki 2.1.2 Priloge B k Direktivi 64/432/EGS.
The test must be executed in the area behind the shoulder (axillary region) following the technique described in point 2.2.4 of Annex B of Directive 64/432/EEC.
Test je treba opraviti na področju za ramo (aksilarno področje) po postopku iz točke 2.2.4 Priloge B k Direktivi 64/432/EGS.
Timing: the animals must be tested within two days from the date of arrival in the quarantine station and 42 days from the date of the first test.
Časovna razporeditev: na živalih je treba opraviti teste v dveh dneh po prihodu živali v karantensko postajo in 42 dni od datuma prvega testa.
Interpretation of tests:
Razlaga testov:
the reaction shall be considered:
reakcijo je treba šteti za:
inconclusive if the increased skin thickness to the bovine PPD is between 2mm and 4 mm, or more than 4 mm but less then the reaction to the avian PPD.
nedoločno, če je povečanje debeline kože na bovini PPD med 2 mm in 4 mm ali večje kot 4 mm, vendar manjše od reakcije na aviarni PPD.
Options for action following testing:
Možni ukrepi po testiranju:
If an animal presents a positive result to the intradermal-reaction to the bovine PPD, that animal shall be excluded from the group and the other animals shall be re-tested starting at least 42 days from the date of the first positive test was administered and this shall be considered as the first test described in (b).
Če se pri živali ugotovi pozitivna reakcija na intradermalno reakcijo bovinega PPD, je treba to žival izključiti iz skupine, na ostalih živalih pa je treba test ponoviti ne prej kot 42 dni od datuma prvega pozitivnega testa, ta test pa se šteje kot prvi test iz točke (b).
If more than one animal of the group presents a positive result, the whole group shall be rejected for exportation to the Union.
Če se za več živali iz skupine ugotovi pozitivna reakcija, se celotna skupina izključi iz izvoza v Unijo.
If one or more animals of the same group present an inconclusive reaction, the whole group shall be re-tested starting at least 42 days from the date of the first test was administered and it shall be considered as the first test described in (b).
Če se pri eni ali več živali iz iste skupine ugotovi nedoločna reakcija, bo na celi skupini test ponovljen ne prej kot 42 dni od datuma prvega testa in se šteje kot prvi test iz točke (b).
Test to be used:
Uporabi se test:
Brucella abortus: Rose Bengal test (RBT) and Serum agglutination test (SAT) as described respectively in points 2.5 and 2.6 of Annex C to Directive 64/432/EEC.
Brucella abortus: rose bengal test (RBT) in test serumske aglutinacije (SAT), kot sta opisana v točkah 2.5 in 2.6 Priloge C k Direktivi 64/432/EGS.
In the case of a positive result, a complement-fixation test shall be performed for confirmation as described in Part 6 of Annex I to Regulation (EU) No 206/2010 (SANCO/4787/2009).
Pri pozitivnem rezultatu je treba zaradi potrditve opraviti test reakcije vezanja komplementa, kot je opisan v delu 6 Priloge I k Uredbi (EU) št. 206/2010 (SANCO/4787/2009).
Brucella melitensis: RBT and SAT as described respectively in points 2.5 and 2.6 of Annex C to Directive 64/432/EEC.
Brucella melitensis: RBT in SAT, kot sta opisana v točkah 2.5 in 2.6 Priloge C k Direktivi 64/432/EGS.
In the case of a positive result, a complement-fixation test following the method described in Annex C to Directive 91/68/EEC shall be performed for confirmation.
Pri pozitivnem rezultatu je treba zaradi potrditve opraviti test reakcije vezanja komplementa po postopku iz Priloge C k Direktivi 91/68/EGS.
Brucella ovis: Complement fixation test as described in Annex D to Directive 91/68/EEC
Brucella ovis: reakcija vezanja komplementa, kot je opisana v Prilogi D k Direktivi 91/68/EGS.