natgillin/translations-raw huggingface dataset:
Foot-and-mouth disease: ELISA test for the detection of antibodies and a virus neutralizaton test within two days after arrival and after at least 42 days
Slinavka in parkljevka: test ELISA za odkrivanje protiteles in nevtralizacijski test za ugotavljanje virusa v dveh dneh po prihodu in nato po najmanj 42 dneh.
Rinderpest: competitive ELISA test within two days after arrival and after at least 42 days
Goveja kuga: kompetitivni test ELISA v dveh dneh po prihodu in nato po najmanj 42 dneh.
Vesicular stomatitis: ELISA or virus- neutralisation test within two days after arrival and after at least 42 days
Vezikularni stomatitis: test ELISA ali nevtralizacijski test za odkrivanje virusa v dveh dneh po prihodu in nato po najmanj 42 dneh.
Rift valley fever: an ELISA test or a virus neutralisation test within two days after arrival and after at least 42 days
Mrzlica doline Rift: test ELISA ali nevtralizacijski test za odkrivanje virusa v dveh dneh po prihodu in nato po najmanj 42 dneh.
Lumpy skin disease: ELISA or virus neutralisation test within two days after arrival and after at least 42 days
Vozličasti dermatitis: test ELISA ali nevtralizacijski test za odkrivanje virusa v dveh dneh po prihodu in nato po najmanj 42 dneh.
Crimean Congo haemorrhagic fever: ELISA or virus neutralisation test within two days after arrival and after at least 42 days
Krimsko-kongoška hemoragična mrzlica: test ELISA ali nevtralizacijski test za odkrivanje virusa v dveh dneh po prihodu in nato po najmanj 42 dneh.
Surra: blood microscopy within two days after arrival and after at least 42 days
Tripanosomiaza: mikroskopski pregled krvi v dveh dneh po prihodu in nato po najmanj 42 dneh.
Malignant catarrhal fever: immunofluorescence test within two days after arrival and after at least 42 days
Maligna kataralna mrzlica: test imunofluorescence v dveh dneh po prihodu in nato po najmanj 42 dneh.
Bovine leukosis: AGID test or ELISA within two days after arrival and after at least 42 days (When required by the EU Member State of destination) (5)
Goveja levkoza: test AGID ali ELISA v dveh dneh po prihodu in nato po najmanj 42 dneh (če to zahteva namembna država članica EU) (5).
either [a treatment with streptomycin 25mg/kg]
ali [zdravljenje s streptomicinom 25 mg/kg]
a vaccination against rabies (if requested) on … (dd/mm/yyyy) using vaccine … (type, producer and lot), and with the test result …]
(5)[II.3.3. cepljenje proti steklini (če se zahteva) dne … (dd/mm/llll) s cepivom …(tip, proizvajalec in serija) in z naslednjim rezultatom testa …]
This certificate is meant for live animals of the family Camelidae.
To spričevalo se uporablja za žive živali iz družine kamel (Camelidae).
Box reference I.28: Species: Select amongst ‘Camelus spp.’, ‘Lama spp.’, ‘Vicugna spp.’ as appropriate.
Rubrika 1.28: Vrsta: ustrezno izberite „Camelus spp.“, „Lama spp.“ ali „Vicugna spp.“.
Animal health certificate for non domestic animals other than Suidae, consigned to the Union (model ‘RUM’) as laid down in Part 2 of Annex I to Regulation (EU) No 206/2010 (SANCO/4787/2009).
Veterinarsko spričevalo za nedomače živali razen družine prašičev (Suidae), ki se odpremljajo v Unijo (vzorec „RUM“), kot je opredeljeno v delu 2 Priloge I k Uredbi (EU) št. 206/2010 (SANCO/4787/2009).
Tests performed in accordance with the methods described in Chapter 2 of Part 7 of Annex I to Regulation (EU) No 206/2010 (SANCO/4787/2009).
Testi, opravljeni v skladu z metodami iz dela 7 poglavja 2 Priloge I k Uredbi (EU) št. 206/2010 (SANCO/4787/2009).
NB: Sampling and testing procedures must be grouped as much as possible while respecting the minimum time intervals to avoid excessive handling and manipulation of the animals.
Opomba:zaradi izogibanja čezmernemu ravnanju in postopkom z živalmi je treba postopke vzorčenja in testiranja izvajati čim bolj združeno ob upoštevanju najmanjših časovnih presledkov.
Addendum for transport of animals by sea
Dodatek za prevoz živali po morju
(To be completed and attached to the veterinary certificate when transport to the Union frontier includes, even for part of the journey, transportation by ship.)
(Izpolnite in priložite veterinarskemu spričevalu, če prevoz na zunanjo mejo Unije zajema ladijski prevoz, čeprav le na delu potovanja.)
(Date of departure)
(Datum odhoda)
(signature of captain)
(podpis vodje)
Conditions for the approval of assembly centres (referred to in Article 4)
Pogoji za odobritev zbirnih centrov (iz člena 4)
In order to be approved, assembly centres must meet the following requirements:
Za odobritev morajo zbirni centri izpolnjevati naslednje zahteve:
I. They must be supervised by an official veterinarian.
I. Morajo biti pod nadzorom uradnega veterinarja.
They must each be situated at the centre of an area of at least 20 km in diameter in which, according to official findings, there has been no case of foot-and-mouth disease for at least a period of 30 days prior to their use as approved assembly centres.
Zbirni center mora ležati sredi območja s premerom vsaj 20 km, na katerem po uradnih ugotovitvah vsaj 30 dni pred uporabo kot odobreni zbirni center ni bilo nobenega primera slinavke in parkljevke.
They must, before each use as approved assembly centres, be cleansed and disinfected with a disinfectant officially authorised in the exporting country as effective for the control of foot-and-mouth disease.
Pred vsako uporabo kot odobreni zbirni center, morajo biti zbirni centri očiščeni in razkuženi z razkužilom, ki je v državi izvoznici uradno odboreno kot učinkovito pri obvladovanju slinavke in parkljevke.
They must have, taking into account their animal capacity:
Ob upoštevanju njihovih zmogljivosti za nastanitev živali morajo imeti:
a facility dedicated exclusively for use as an assembly centre;
prostor, predviden izključno za uporabo kot zbirni center;
appropriate facilities, that are easy to clean and disinfect, for loading, unloading and adequate housing of a suitable standard for the animals, for watering and feeding them, and for giving them any necessary treatment;
ustrezne objekte in naprave, enostavne za čiščenje in razkuževanje, za natovarjanje in raztovarjanje ter opremo primernega standarda za nastanitev živali, za napajanje in hranjenje živali ter potrebno oskrbo;
appropriate facilities for inspection and isolation;
primerne prostore za veterinarske inšpekcijske preglede in izolacijo živali;
appropriate equipment for cleaning and disinfecting rooms and trucks;
ustrezno opremo za čiščenje in razkuževanje prostorov in tovornjakov;
an appropriate storage area for fodder, litter and manure;
primerne skladiščne prostore za krmo, steljo in gnoj;
an appropriate system for collecting and disposal of waste water;
ustrezen sistem za zbiranje in odstranjevanje odpadne vode;
an office for the official veterinarian.
pisarno za uradnega veterinarja.
V. When operating, they must have sufficient veterinarians to carry out all duties set out in Part 5;
V. Kadar obratujejo, mora biti na voljo dovolj veterinarjev za izvajanje vseh nalog iz dela 5.
They must only admit animals that are individually identified so as to guarantee traceability.
Sprejemati smejo le posamezno označene živali, da je zagotovljena sledljivost.
To this end, when animals are admitted the owner or the person in charge of the centre must ensure that the animals are properly identified and accompanied by health documents or certificates for the species and categories involved.
V ta namen mora lastnik centra ali oseba, odgovorna za center, ob sprejemanju živali zagotoviti, da so pravilno označene in jih spremljajo zdravstveni dokumenti ali spričevala za zadevne živalske vrste ali kategorije.
In addition, the owner or the person in charge of the assembly centre must record on a register or in a data base, and retain for at least three years the name of the owner, the origin of the animals, the dates of entry and exit, the identification number of the animals or registration number of the herd of origin and the holding of destination, and, the registration number of the carrier and the registration number of the lorry delivering or collecting animals from that assembly centre.
Poleg tega mora lastnik zbirnega centra ali oseba, odgovorna za zbirni center, v registru ali podatkovni bazi evidentirati in vsaj tri leta hraniti ime lastnika, izvor živali, datum vpisa in izpisa, identifikacijsko številko živali ali številko registracije črede izvora in namembno gospodarstvo ter številko registracije prevoznika in registrsko številko tovornjaka, ki živali pripelje v zbirni center ali jih odpelje iz njega.
All animals passing through the assembly centre must fulfil the health conditions established for the introduction of the relevant category of animal into the Union.
Vse živali, ki potujejo prek zbirnega centra, morajo izpolnjevati zdravstvene pogoje, predpisane za vnos ustrezne kategorije živali v Unijo.
Animals to be introduced into the Union which pass through an assembly centre must, within six days of arrival at the assembly centre, be loaded and dispatched directly to the border of the exporting country:
Živali, namenjene za vnos v Unijo, ki potujejo prek zbirnega centra, morajo biti v šestih dneh po prispetju v zbirni center natovorjene in odposlane neposredno na zunanjo mejo države izvoznice:
without coming into contact with cloven-hoofed animals other than animals which fulfil the health conditions established for the introduction of the relevant category of animal into the Union;
pri tem ne smejo priti v stik s parkljarji, razen tistimi, ki izpolnjujejo zdravstvene pogoje, predpisane za vnos ustrezne kategorije živali v Unijo;
segregated into consignments so that no consignment contains both animals for breeding or production and animals for immediate slaughter;
po pošiljkah so ločene tako, da nobena pošiljka ne vsebuje hkrati živali za vzrejo ali proizvodnjo ter živali za takojšnji zakol;
in transport vehicles or containers which have first been cleansed and disinfected with a disinfectant officially authorised in the exporting country as effective for the control of foot-and-mouth disease and which are so constructed that faeces, urine, litter or fodder cannot flow or fall out during transportation.
v prevoznih sredstvih ali zabojnikih, prej očiščenih in razkuženih z razkužilom, ki je v državi izvoznici uradno odobreno kot učinkovito pri obvladovanju slinavke in parkljevke in zgrajenih tako, da iztrebki, urin, stelja ali krma ne morejo iztekati ali izpadati med prevozom.
Where the conditions for the export of animals to the Union require that a test is carried out within a specified period before loading, that period must include any period of assembly, up to six days, from the date of arrival of the animals at the approved assembly centre.
Kadar pogoji za izvoz živali v Unijo zahtevajo, da se živali testirajo v določenem obdobju pred natovarjanjem, mora to obdobje zajemati tudi obdobje bivanja v zbirnem centru, do šest dni, po prispetju živali v odobreni zbirni center.
The exporting third country must designate the centres which are approved for animals for breeding and production and those centres which are approved for animals for slaughter and must notify the Commission and the competent central authorities of the Member States of the names and addresses of such premises.
Tretja država izvoznica mora imenovati centre, ki so odobreni za živali za vzrejo in proizvodnjo, in centre, ki so odobreni za živali za zakol, ter mora obveščati Komisijo in glavne pristojne organe držav članic o imenih in naslovih navedenih centrov.
That information must be updated regularly.
Te informacije je treba redno posodabljati.
The exporting third country shall determine the procedure for official supervision of approved assembly centres and shall ensure that such supervision is carried out.
Tretja država izvoznica določi postopek uradnega nadzora odobrenih zbirnih centrov in zagotovi, da se tak nadzor izvaja.
The approved assembly centres must be regularly inspected by the competent authority of the third country in order to check that the requirements for approval set out in points I to XI continue to be fulfilled.
Odobrene zbirne centre mora redno pregledovati pristojni organ tretje države zaradi zagotovitve, da so zahteve v zvezi z odobritvijo iz točk I do XI še naprej izpolnjujejo.
If those inspections show that those conditions are no longer complied with, the approval of the centre must be suspended.
Če navedeni pregledi pokažejo, da se pogoji ne upoštevajo več, se mora odobritev za center začasno preklicati.
The approval may be restored only when the competent authority of the third country is satisfied that the centre fully complies with the conditions set out in points I to XI.
Odobritev se lahko obnovi le, če je pristojni organ tretje države prepričan, da center v celoti izpolnjuje pogoje iz točk I do XI.
Protocols for the standardisation of materials and testing procedures
Protokoli za standardizacijo snovi in postopkov testiranja
Tuberculosis (TBL)
Tuberkuloza (TBC)
The single intradermal tuberculin test using bovine tuberculin shall be carried out according to Annex B to Directive 64/432/EEC.
Intradermalni tuberkulinski monotest z bovinim tuberkulinom se izvede v skladu s Prilogo B k Direktivi 64/432/EGS.
In the case of Suidae animals, the single intradermal tuberculin test using avian tuberculin shall be carried out according to Annex B to 64/432/EEC, except that the site of injection shall be the loose skin at the base of the ear.
V primeru živali iz družine Suidae se intradermalni tuberkulinski monotest z aviarnim tuberkulinom izvede v skladu s Prilogo B k Direktivi 64/432/EGS, s to razliko, da je mesto vbrizganja ohlapna koža ob korenu uhlja.
The serum agglutination test, complement fixation test, buffered brucella antigen test and enzyme linked immuno-absorbent assays tests (ELISA) shall be carried out according to Annex C to Directive 64/432/EEC.
Test serumske aglutinacije, reakcija vezanja komplementa, test s puferiranim antigenom in encimskoimunska metoda (test ELISA) se izvajajo v skladu s Prilogo C k Direktivi 64/432/EGS.
Tests shall be carried out according to Annex C to Directive 91/68/EEC.
Testi se izvajajo v skladu s Prilogo C k Direktivi 91/68/EGS.
Enzootic Bovine Leukosis (EBL)
Enzootska goveja levkoza (EGL)
The agar gel immuno-diffusion test and the enzyme linked immuno-absorbent assay test (ELISA) shall be carried out according to paragraphs A and C of Chapter II of Annex D to Directive 64/432/EEC.
Agar gel imunodifuzijski test in encimskoimunska metoda (test ELISA) se izvajata v skladu z odstavkoma A in C poglavja II Priloge D k Direktivi 64/432/EGS.
Bluetongue (BTG)
Bolezen modrikastega jezika (BT)
The blocking or competitive ELISA test shall be carried out according to the following protocol:
Blocking ali kompetitivna ELISA se izvaja v skladu z naslednjim protokolom:
The competitive ELISA using monoclonal antibody 3-17-A3 is capable of identifying antibodies to all known serotypes of bluetongue virus (BTV).
Kompetitivna ELISA z monoklonskim protitelesom 3-17-A3 se uporablja za ugotavljanje protiteles za vse znane serotipe virusa bolezni modrikastega jezika (BTV).
The principle of the test is the interruption of the reaction between BTV antigen and a group-specific monoclonal antibody (3-17-A3) by the addition of test serum.
Načelo testa je prekinitev reakcije med antigenom virusa bolezni modrikastega jezika (BTV) ter grupno specifičnim monoklonskim protitelesom (3-17-A3) ob dodatku preiskovanega seruma.
Antibodies to BTV present in the test serum block the reactivity of the monoclonal antibody (Mab) and result in a reduction in the expected colour development after the addition of enzyme labelled anti-mouse antibody, and chromogen/ substrate.
Protitelesa proti BTV, prisotna v preiskovanem serumu, ustavijo reaktivnost monoklonskega protitelesa (Mab), kar povzročijo zmanjšanje pričakovanega obarvanja po dodatku z encimom označenega protimišjega protitelesa in kromogena/substrata.
Sera can be tested at a single dilution of 1:5 (spot test – Appendix 1) or may be titrated (serum titration – Appendix 2) to give dilution end-point.
Serumi se lahko testirajo v enkratni razredčitvi 1:5 (enkratna redčitev – Dodatek 1) ali pa se titrirajo (titracija seruma – Dodatek 2), da dobimo končne titre razredčitev seruma.
Material and Reagents:
Materiali in reagenti:
Appropriate ELISA microtitre plates.
Ustrezne mikroplošče za test ELISA.
Antigen: supplied as a cell extracted concentrate, prepared as described below, and stored at either –20 °C or –70 °C.
Antigen: koncentriran ekstrakt celic, ki se pripravi, kakor je opisano v nadaljevanju, in se hrani pri –20 °C ali –70 °C.
Blocking buffer: phosphate buffered saline (PBS) containing 0,3 % BTV negative adult bovine serum, 0,1 % (v/v) Tween-20 (supplied as polyoxyethylene sorbiton monolaurate syrup) in PBS.
Bloking pufer: fosfatni pufer s soljo (PBS), ki vsebuje 0,3 % BTV negativnega bovinega seruma (odraslih govedi), 0,1 % (vol:vol) Tween-20 (na voljo kot sirup polioksietilen sorbiton monolaurata) v PBS.
Monoclonal antibody: 3-17-A3 (supplied as hybridoma tissue-culture supernatant) directed against the group-specific polypeptide VP7, stored at -20 °C or freeze-dried and diluted 1/100 with blocking buffer before use.
Monoklonalno protitelo: 3-17-A3 (na voljo kot supernatant kulture tkiva hibridoma), usmerjeno na grupno specifični polipeptid VP7, shranjeno pri –20 °C ali liofilizirano in pred uporabo razredčeno v razmerju 1:100 s pufrom za ustavitev reakcije.
Test Sera
Testni serumi
APPENDIX 2:
DODATEK 2
Serum titration format (10 sera/plate)
Titracija seruma (10 serumov/ploščo)
Test protocol:
Testni protokol:
Conjugate control (Cc) Wells 1A and 1B are a blank control consisting of BTV antigen and conjugate.
Kontrola konjugata (Cc) jamici 1A in 1B sta slepa kontrola, ki je sestavljena iz antigena proti BTV in konjugata.
This may be used to blank the ELISA reader.
Uporabi se lahko za izravnavo čitalnika testa ELISA.
Mab control (Cm) Columns 1 and 2, rows G and H are the monoclonal antibody control and contain BTV antigen, monoclonal antibody and conjugate.
Kontrola Mab (Cm) stolpca 1 in 2, vrstici G in H, sta kontrola z monoklonskim protitelesom in vsebujeta antigen proti BTV, monoklonsko protitelo in konjugat.
These wells represent maximum colour.
V teh jamicah je obarvanje najbolj intenzivno.
The mean of the optical density readings from this control represents the 0 % inhibition value.
Srednja vrednost odčitanj optične gostote iz te kontrole je vrednost inhibicije 0 %.
Positive control (C++, C+) Columns 1 and 2, rows C-D-E-F.
Pozitivna kontrola (C++, C+) stolpca 1 in 2, vrstice C-D-E-F.
These wells contain BTV antigen, BTV strong and weak positive antiserum respectively, Mab and conjugate.
Te jamice vsebujejo antigen proti BTV, močno pozitivno serumsko kontrolo proti BTV oziroma šibko pozitivno serumsko kontrolo proti BTV, Mab in konjugat.
Negative control (C-) Wells 2A and 2B are the negative controls, which contain BTV antigen, BTV negative antiserum, Mab and conjugate.
Negativna kontrola (C-) jamici 2A in 2B sta negativni kontroli, ki vsebujeta antigen proti BTV, negativni serum proti BTV, Mab in konjugat.
This will give some indication of the titre of antibody in the test sera.
Tako lahko približno določimo titer protitelesa v preiskovanem serumu.
Dilute BTV antigen to pre-titrated concentration in PBS, sonicate briefly to disperse aggregated virus (if sonicator is not available, pipette vigorously) and add 50 μl to all wells of the ELISA plate.
Antigen proti BTV razredčimo na predtitrirano koncentracijo v PBS, ga damo za kratek čas v sonikator, da se nakopičeni virus porazdeli (če sonikator ni na voljo, antigen porazdelimo s pipetiranjem), in dodamo po 50 μl v vse jamice na mikroplošči za test ELISA.
Tap sides of plate to disperse antigen.
Mikroploščo stresamo levo in desno, da se antigen enakomerno porazdeli.
Incubate at 37 °C for 60 minutes on an orbital shaker.
Inkubiramo pri 37 °C 60 minut na orbitalnem stresalniku.
Wash plates three times by flooding and emptying the wells with non-sterile PBS and blot dry on absorbent paper.
Mikroplošče trikrat speremo s polnjenjem in praznjenjem jamic z nesterilnim PBS in jih osušimo na vpojnem papirju.
Control wells: Add 100 μl of blocking buffer to Cc wells.
Kontrolne jamice: dodamo 100 μl bloking pufra v vse jamice Cc.
Add 50 ul of positive and negative control sera, at a dilution of 1:5 (10 μ l sera + 40 μl blocking buffer), to respective wells C-, C+ and C++.
Dodamo 50 μl pozitivnega in negativnega kontrolnega seruma v razredčitvi 1:5 (10 μl serumov +40 μl pufra za ustavitev reakcije), v vsako posamezno jamico C-, C + in C++.
Add 50μl blocking buffer to Mab control wells.
Dodamo 50 μl bloking pufra v jamice za kontrolo Mab.
Spot titration method: Add a 1:5 dilution of each test serum in blocking buffer to duplicate wells of columns 3 to 12 (10 μl sera +40 μl blocking buffer),
Metoda enkratne redčitve: testni serum, razredčen 1:5 v bloking pufru, damo v paralelne jamice v stolpcih 3 do 12 (10 μl serumov +40 μl pufra za ustavitev reakcije),
Serum titration method: Prepare a two-fold dilution series of each test sample (1:5 to 1:640) in blocking buffer across eight wells of single columns 3 to 12.
metoda serumske titracije: pripravimo zaporedje dvojnih redčitev vsakega preiskovanega serumskega vzorca (1:5 do 1:640) v pufru za ustavitev reakcije, v osmih jamicah enojnih stolpcev 3 do 12.
Immediately after the addition of the test sera, dilute Mab 1:100 in blocking buffer and add 50 μl to all wells of the plate except for the blank control.
Takoj po dodatku preiskovanih serumov razredčimo Mab 1:100 v bloking pufru in ga dodamo po 50 μl v vsako jamico na mikroplošči, razen slepe kontrole.
Wash three times with PBS and blot dry.
Trikrat speremo s PBS in osušimo na vpojnem papirju.
Dilute rabbit anti-mouse concentrate to 1/5000 in blocking buffer and add 50 μl to all wells of the plate.
Razredčimo kunčji protimišji koncentrat na 1:5000 v bloking pufru in ga dodamo po 50 μl v vsako jamico na mikroplošči.
Thaw the O-Phenylenediamine dihydrochloride (OPD) and immediately before use add 5 μl of 30 % hydrogen peroxide to each 10 ml of OPD.
Odtajamo O-fenilendiamin dihidroklorid (OPD) in tik pred uporabo dodamo 5 μl 30-odstotnega vodikovega peroksida v vsakih 10 ml OPD.
Add 50 μl to all wells of the plate.
Dodamo po 50 μl v vsako jamico na mikroplošči.
Allow colour to develop for approximately 10 minutes and stop the reaction with 1 Molar sulphuric acid (50 μl per well).
Počakamo približno 10 minut, da se jamice obarvajo, in ustavimo reakcijo z 1 molarno žvepleno kislino (50 μl na jamico).
Colour should develop in the Mab control wells and in those wells containing sera with no antibody to BTV.
Obarvati se morajo kontrolne jamice Mab ter jamice, v katerih so serumi brez protitelesa proti BTV.
Examine and record the plates either visually or using a spectrophotometric reader.
Mikroplošče pregledamo vidno ali s spektrofotometričnim čitalnikom in zapišemo rezultate.
Analysis of results:
Analiza rezultatov:
Using the software package print out the optical density (OD) values, and the percentage inhibition (PI) for test and control sera based on the mean value recorded in the antigen control wells.
Z ustreznim računalniškim programskim paketom izpišemo vrednosti optične gostote (OD) in odstotke inhibicije (PI) za testne in kontrolne serume na podlagi srednje vrednosti, ugotovljene v kontrolnih jamicah za antigen.
The date expressed as OD and PI values are used to determine whether the test has performed within acceptable limits.
Podatke, izražene kot vrednosti OD in PI, uporabimo za ugotavljanje, ali je test opravljen znotraj sprejemljivih kriterijev.
If a computer software package is not available print out the OD values using the ELISA printer.
Če računalniški programski paket ni na voljo, izpišemo vrednosti OD na tiskalniku testa ELISA.
Calculate the mean OD value for the antigen control wells, which is equivalent to the 100 % value.
Izračunamo srednjo vrednost OD za kontrolne jamice za antigen, ki je enaka 100-odstotni vrednosti.
Determine the 50 % OD value and manually calculate the positivity and negativity of each sample.
Določimo 50-odstotno vrednost OD in ročno izračunamo pozitivnost in negativnost vsakega vzorca.