natgillin/translations-raw huggingface dataset:
To fortify at a level of 50 mg/kg, transfer 5,0 ml of the stock standard solution (3.11.1) to a 200 ml conical flask.
Za koncentracijo 50 mg/kg se 5,0 ml osnovne standardne raztopine (3.11.1) prenese v 200-mililitrsko erlenmajerico.
Evaporate the solution to approximately 0,5 ml in a stream of nitrogen.
Raztopina se upari na približno 0,5 ml v toku dušika.
Add 10 g of the blank feed, mix and wait for 10 minutes before proceeding with the extraction step (5.2).
Doda se 10 g slepe krme, premeša se in pusti 10 minut, nato pa se nadaljuje z ekstrakcijo (5.2).
In this case, the sample is fortified with a quantity of carbadox, similar to that already present in the sample.
V tem primeru se vzorcu za analizo doda količina karbadoksa, podobna količini v vzorcu.
Weigh to the nearest 0,01 g, 10 g of the sample and transfer to a 200 ml conical flask.
10 g vzorca se natehta na 0,01 g natančno in prenese v 200-mililitrsko erlenmajerico.
Add 15,0 ml of water, mix, and equilibrate for 5 min. Add 35,0 ml of methanol-acetonitrile (3.5), stopper and shake for 30 min. on the shaker or stir on the magnetic stirrer (4.1).
Doda se 15,0 ml vode, premeša se in pusti 5 minut, da se uravnoteži. Doda se 35,0 ml metanol-acetonitrila (3.5), zamaši se in stresa 30 minut na stresalniku ali meša z magnetnim mešalnikom (4.1).
Filter the solution through a glass fibre filter paper (4.2).
Raztopina se filtrira skozi filtrirni papir iz steklenih vlaken (4.2).
Premixtures (0,1 %-2,0 %)
Premiksi (0,1–2,0 %)
Weigh to the nearest 0,001 g, 1 g of the unground sample and transfer to a 200 ml conical flask.
1 g nezdrobljenega vzorca se natehta na 0,001 g natančno in prenese v 200-mililitrsko erlenmajerico.
Pipet an aliquot of filtrate into a 50 ml calibrated flask.
Alikvot filtrata se odpipetira v 50-mililitrsko merilno bučko.
Add 15,0 ml of water, make up to the mark with methanol-acetonitrile (3.5) and mix.
Doda se 15,0 ml vode, dopolni se z metanol-acetonitrilom (3.5) do oznake in premeša.
The carbadox concentration of the final solution shall be approximately 10 μg/ml.
Koncentracija karbadoksa v končni raztopini je približno 10 μg/ml.
An aliquot is filtered through a 0,45 μm filter (4.6).
Alikvot se filtrira skozi filter 0,45 μm (4.6).
Proceed to the HPLC determination (5.4).
Nadaljuje se z določanjem s HPLC (5.4).
Preparations (> 2 %)
Pripravki (> 2 %)
Weigh to the nearest 0,001 g, 0,2 g of the unground sample and transfer to a 250 ml conical flask.
0,2 g nezdrobljenega vzorca se natehta na 0,001 g natančno in prenese v 250-mililitrsko erlenmajerico.
Add 45,0 ml of water, mix, and equilibrate for 5 min. Add 105,0 ml of methanol-acetonitrile (3.5), stopper and homogenise.
Doda se 45,0 ml vode, premeša se in pusti 5 minut, da se uravnoteži. Doda se 105,0 ml metanol-acetonitrila (3.5), zamaši se in homogenizira.
Sonicate (4.7) the sample for 15 min. followed by shaking or stirring for 15 min. (4.1).
Vzorec se pusti v ultrazvočni kopeli (4.7) 15 minut, nato pa se 15 minut stresa ali meša (4.1).
Dilute an aliquot of filtrate with the mixture of water-methanol-acetonitrile (3.12) to a final carbadox concentration of 10-15 μg/ml (for a 10 % preparation, the dilution factor is 10).
Alikvot filtrata se razredči z mešanico vode, metanola in acetonitrila (3.12), da je končna koncentracija karbadoksa 10–15 μg/ml (za 10-odstotni preparat je faktor razredčitve 10).
Preparation of the aluminium oxide column
Priprava kolone iz aluminijevega oksida
Weigh 4 g of aluminium oxide (3.4) and transfer it to the glass column (4.3).
Natehta se 4 g aluminijevega oksida (3.4) in prenese v stekleno kolono (4.3).
Apply 15 ml of the filtered extract (5.2.1) to the aluminium oxide column and discard the first 2 ml of eluate.
15 ml filtriranega ekstrakta (5.2.1) se nanese na kolono iz aluminijevega oksida, prva 2 ml eluata pa se zavržeta.
Collect the next 5 ml and filter an aliquot through a 0,45 μm filter (4.6).
Zbere se naslednjih 5 ml, alikvot pa se filtrira skozi filter 0,45 μm (4.6).
column (4.4.1):
kromatografijo (4.4.1):
300 mm × 4 mm, C18, 10 μmpacking or equivalent
300 mm × 4 mm, C18, polnitev 10 μm ali enakovredna
Mobile phase (3.10):
Mobilna faza (3.10):
Mixture of acetate buffer solution (3.9) and acetonitrile (3.2), 825 + 175 (v+v)
Mešanica raztopine acetatnega pufra (3.9) in acetonitrila (3.2), 825 + 175 (v+v)
Check the stability of the chromatographic system, injecting the calibration solution (3.11.2) containing 5,0 μg/ml several times, until constant peak heights (areas) and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizgavanjem raztopine za umerjanje (3.11.2) s koncentracijo 5,0 μg/ml, dokler se ne dosežejo konstantne višine vrhov (površin) in konstantni retencijski časi.
Inject each calibration solution (3.11.2) several times and measure the peak heights (areas) for each concentration.
Vsaka raztopina za umerjanje (3.11.2) se večkrat vbrizga in za vsako koncentracijo se določijo srednje vrednosti višin (površin) vrhov.
Plot a calibration curve using the mean peak heights or areas of the calibration solutions as the ordinates and corresponding concentrations in μg/ml as the abscissae.
Umeritvena krivulja se pripravi tako, da se na ordinato nanesejo srednje vrednosti višin ali površin vrhov raztopin za umerjanje, na absciso pa ustrezne koncentracije v μg/ml.
Inject the sample extract [(5.3.2) for feed, (5.2.2) for premixtures and (5.2.3) for preparations] several times and determine the mean peak height (area) of the carbadox peaks.
Ekstrakt vzorca [(5.3.2) za krmo, (5.2.2) za premikse in (5.2.3) za pripravke] se vbrizga večkrat, nato pa se določijo srednje vrednosti višin (površin) vrhov karbadoksa.
From the mean height (area) of the carbadox peaks of the sample solution determine the carbadox concentration of the sample solution in μg/ml by reference to the calibration graph (5.4.2).
Koncentracija karbadoksa v raztopini vzorca v μg/ml se določi iz srednje vrednosti višin (površin) vrhov karbadoksa iz umeritvene krivulje (5.4.2).
The content of carbadox w (mg/kg) in the sample is given by the following formula:
Vsebnost karbadoksa (w) (mg/kg) v vzorcu se izračuna po naslednji formuli:
c carbadox concentration of the sample extract (5.3.2) in μg/ml
c koncentracija karbadoksa v ekstraktu vzorca (5.3.2) v μg/ml;
extraction volume in ml (i.e. 50)
volumen ekstrakta v ml (tj. 50);
Premixtures and preparations
Premiksi in pripravki
c carbadox concentration of the sample extract (5.2.2 or 5.2.3) in μg/ml
c koncentracija karbadoksa v ekstraktu vzorca (5.2.2 ali 5.2.3) v μg/ml;
extraction volume in ml (i.e. 50 for premixtures; 150 for preparations)
volumen ekstrakta v ml (tj. 50 za premikse; 150 za pripravke);
f dilution factor according to 5.2.2 (premixtures) or 5.2.3 (preparations)
f faktor razredčitve v skladu s točko 5.2.2 (premiksi) ali 5.2.3 (pripravki);
The identity of the analyte can be confirmed by co-chromatography, or by using a diode-array detector by which the spectra of the sample extract and the calibration solution (3.11.2) containing 10,0 μg/ml are compared.
Identiteta analita se lahko potrdi z dodatno kromatografijo ali detektorjem s serijo diod, s katerim se primerjata spekter ekstrakta vzorca in spekter raztopine za umerjanje (3.11.2) s koncentracijo 10,0 μg/ml.
A sample extract is fortified by addition of an appropriate amount of calibration solution (3.11.2).
Ekstraktu vzorca se doda primerna količina raztopine za umerjanje (3.11.2).
The amount of added carbadox must be similar to the estimated amount of carbadox found in the sample extract.
Količina dodanega karbadoksa mora biti podobna ocenjeni količini karbadoksa v ekstraktu vzorca.
Only the height of the carbadox peak shall be enhanced after taking into account both the amount added and the dilution of the extract.
Povišan je le vrh karbadoksa ob upoštevanju dodane količine in razredčitve ekstrakta.
For diode-array detection, this is typically within + 2 nm;
Za detekcijo s serijo diod je to ponavadi znotraj ± 2 nm;
between 225 and 400 nm, the sample and standard spectra recorded at the peak apex on the chromatogram, must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
med 225 in 400 nm se spekter vzorca in spekter standarda na najvišji točki kromatografskega vrha za navedene dele spektra ne smeta razlikovati znotraj območja 10–100 % relativne absorbance.
between 225 and 400 nm, the spectra of the upslope, apex and downslope of the peak produced by the sample extract must not be different from each other for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
med 225 in 400 nm se spektri naraščanja, najvišje točke vrha in padanja vrha, pridobljeni iz ekstrakta vzorca, za navedene dele spektra ne smejo razlikovati med seboj v območju 10–100 % relativne absorbance.
For contents of 10 mg/kg and higher, the difference between the results of two parallel determinations carried out on the same sample must not exceed 15 % relative to the higher result.
Razlika med rezultatoma dveh vzporednih določanj, izvedenih na istem vzorcu, za vsebnosti 10 mg/kg in več ne sme presegati 15 % višjega rezultata.
A collaborative study was arranged in which 6 feed, 4 premixtures and 3 preparations were analysed by 8 laboratories.
Organizirana je bila medlaboratorijska študija, pri kateri je 8 laboratorijev analiziralo 6 vzorcev krme, 4 vzorce premiksov in 3 vzorce pripravkov.
(More detailed information on this collaborative study can be found in the Journal of the AOAC, Volume 71, 1988, p. 484-490).
(Za natančnejše informacije o medlaboratorijski študiji glej Journal of AOAC, knjiga 71, št. 1988, 484, str. 484–490.)
The results (excluding outliers) are shown below:
Rezultati (razen izjem) so prikazani v spodnji preglednici:
Table 1
Preglednica 1.
Results of the collaborative study for feed
Rezultati medlaboratorijske študije za krmo
Mean (mg/kg)
Srednja vrednost (mg/kg)
Table 2
Preglednica 2.
Results of the collaborative study for premixtures and preparations
Rezultati medlaboratorijske študije za premikse in pripravke
Mean (g/kg)
Srednja vrednost (g/kg)
Nominal content (g/kg)
Nominalna vsebnost (g/kg)
Sr standard deviation of repeatability
S r standardni odmik ponovljivosti;
CVr coefficient of variation of repeatability
CV r koeficient variacije ponovljivosti;
SR standard deviation of reproducibility
S R standardni odmik obnovljivosti;
CVR coefficient of variation of reproducibility.
CV R koeficient variacije obnovljivosti.
CORRELATION TABLES REFERRED TO IN ARTICLE 6
KORELACIJSKE TABELE IZ ČLENA 6
Directive 71/250/EEC
Direktiva 71/250/EGS
Article 1 first subparagraph
Člen 1, prvi pododstavek
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Annex, part 1
Priloga, del 1
Annex, part 2
Priloga, del 2
Annex, part 3
Priloga, del 3
Annex, part 4
Priloga, del 4
Annex III, part O
Priloga III, del O
Annex, part 5
Priloga, del 5
Annex III, part M
Priloga III, del M
Annex, part 6
Priloga, del 6
Annex III, part N
Priloga III, del N
Annex, part 7
Priloga, del 7
Annex III, part Q
Priloga III, del Q
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Priloga III, del K
Annex, part 10
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Annex, part 11
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Each aggregate sample gives the final samples on reduction.
Iz vsakega skupnega vzorca se po zmanjšanju oblikuje končni vzorec.
Analysis of at least one final sample per aggregate sample is required.
Zahteva se analiza vsaj enega končnega vzorca na vzorčeni delež.
Where a fully automatic distillation unit is employed, the automatic titration of the ammonia can also be carried out with end-point detection using a potentiometric pH system.
Pri popolnoma avtomatizirani destilacijski enoti se lahko izvaja avtomatična titracija amonijaka, ekvivalentna točka pa se lahko določa s potenciometričnim pH sistemom.
Add 50 ml of trichloroacetic acid solution (3.1), make up to volume with water, shake vigorously and filter through a pleated filter.
Doda se 50 ml raztopine trikloroocetne kisline (3.1), dopolni z vodo do oznake, močno pretrese in filtrira skozi nagubani filter.
Dissolve 19,61 g sodium citrate (3.8), 5 ml thiodiglycol (3.9), 1 g phenol (3.3) and 16,50 ml HCl (3.7) in approximately 800 ml water. Adjust pH to 2,20.
v približno 800 ml vode se raztopi 19,61 g natrijevega citrata (3.8), 5 ml tiodiglikola (3.9), 1 g fenola (3.3) in 16,50 ml HCl (3.7). pH se uravna na 2,20.
Dissolve 2,2 g sodium chloride (3.10) in 100 ml beaker with 30 ml citrate buffer (3.24).
V 100-mililitrski čaši se raztopi 2,2 g natrijevega klorida (3.10) s 30 ml citratnega pufra (3.24).
Add 4,00 ml stock standard solution of amino acids (3.27.1), 4,00 ml stock standard solution of cysteic acid and methionine sulphone (3.27.2) and 0,50 ml stock standard solution of internal standard (3.27.3) if used. Adjust pH to 2,20 with sodium hydroxide (3.18).
Dodajo se 4,00 ml osnovne standardne raztopine aminokisline (3.27.1), 4,00 ml osnovne standardne raztopine cisteinske kisline in metionin-sulfona (3.27.2) ter 0,50 ml osnovne standardne raztopine notranjega standarda (3.27.3), če se uporablja. pH se uravna na 2,20 z natrijevim hidroksidom (3.18).
Mobile phase for HPLC: 3,00 g acetic acid (3.18) + 900 ml water (3.1) +50,0 ml solution (3.21) of 1,1,1-trichloro-2-methyl-2-propanol (3.19) in methanol (3.8) (1g/100ml). Adjust pH to 5,00 using ethanolamine (3.20).
Mobilna faza za HPLC: 3,00 g ocetne kisline (3.18) + 900 ml vode (3.1) +50,0 ml raztopine (3.21) 1,1,1-trikloro-2-metil-2-propanola (3.19) v metanolu (3.8) (1 g/100 ml). pH se uravna na 5,00 z etanolaminom (3.20).
Add 5 ml ortho-phosphoric acid (3.14). Adjust the pH to 3 using sodium hydroxide (3.10).
Doda se 5 ml ortofosforne kisline (3.14). pH raztopine se z natrijevim hidroksidom (3.10) uravna na 3.
Transfer to an autoclave (4.6) with boiling water and steam for 30-60 minutes.
Prenese se v avtoklav (4.6), kjer je vrela voda, in pusti v pari 30–60 minut.
Close the autoclave and autoclave at 110 (± 2) oC for 20 hours.
Zapre se avtoklav in 20 ur avtoklavira pri 110 (± 2) oC.
3,00 g acetic acid (3.18) + 900 ml water (3.1) +50,0 ml solution (3.21) of 1,1,1-trichloro-2- methyl-2-propanol (3.19) in methanol (3.8) (1 g/100 ml). Adjust pH to 5,00 using ethanolamine (3.20).
3,00 g ocetne kisline (3.18) + 900 ml vode (3.1) +50,0 ml raztopine (3.21) 1,1,1-trikloro-2-metil-2-propanola (3.19) v metanolu (3.8) (1 g/100 ml). pH raztopine se z etanolaminom (3.20) uravna na 5,00.
Replicate (5) analyses were performed on each sample. The results are given in the following table:
Rezultati so prikazani v spodnji preglednici:
Pre-extraction by Procedure A prior to hydrolysis and re-extraction by Procedure B is recommended if the crude oil/fat content is greater than 15 %.
Če je vsebnost surovih olj/maščob višja od 15 %, se pred hidrolizo po postopku A priporoča predhodna ekstrakcija, pri postopku B pa ponovna ekstrakcija.
To some extent this depends on the nature of the feed and the nature of the oil/fat in the feed.
Do določene mere je to odvisno od vrste krme ter vrste olj in maščob v krmi.
Bring the liquid to boiling point within 5 ± 2 minutes and boil vigorously for exactly 30 minutes.
Tekočina se zavre v 5 ± 2 minutah ter pusti močno vreti natančno 30 minut.
Filter and repeat the washing procedure used for the sulphuric acid step.
To se filtrira, postopek spiranja pa se ponovi, kakor je bil uporabljen za žveplovo kislino.
If the feed contains over 5 % of carbonates, expressed as calcium carbonate, connect the crucible (4.2) with the weighed sample to the heating unit (4.1).
Če krma vsebuje več kot 5 % karbonatov, izraženih kot kalcijev karbonat, se lonček (4.2) z natehtanim vzorcem poveže z enoto za segrevanje (4.1).
Wash the sample three times with 30 ml hydrochloric acid (3.6).
Vzorec se trikrat spere s po 30 ml klorovodikove kisline (3.6).
Check the concentration of the reagent thus obtained (Cu 0,05 mol/litre; Na2 CO3 1 mol/litre), see (5.4) last paragraph. The solution's pH shall be approximately 9,4.
Preveri se koncentracija tako dobljenega reagenta (Cu 0,05 mol/l; Na2 CO3 1 mol/l), glej zadnji odstavek točke 5.4. pH raztopine je približno 9,4.
For an accurate calculation of reducing sugars, excluding lactose, the same sample must be used for the two final determinations.
za natančen izračun reducirajočih sladkorjev, razen laktoze, je treba za končno določitev uporabiti isti vzorec.
One of the analyses is carried out on part of the solution obtained under 5.1, the other on part of the solution obtained during the determination of lactose by the method laid down for that purpose (after fermenting the other types of sugar and clarifying).
Ena od analiz se izvede na delu raztopine, ki nastane pri postopku iz točke 5.1, druga pa na delu raztopine, ki nastane pri določanju laktoze z metodo, določeno za navedeni namen (po fermentiranju drugih vrst sladkorja in zbistritve).
Check the concentration of the reagent thus obtained (Cu 0,05 mol/litre; Na2 CO3 1 mol/litre). The solution's pH shall be approximately 9,4.
Preveri se koncentracija tako dobljenega reagenta (Cu 0,05 mol/l, Na2 CO3 1 mol/l). pH raztopine je približno 9,4.
Carbonise by setting light to the substance with a strip of ash-free filter paper.
Upepeli se tako, da se snov zažge s trakom iz filtrirnega papirja brez pepela.
After combustion, moisten with as little water as possible.
Po sežigu se navlaži s čim manj vode.
Place the test sample in a Kjeldahl flask, add 20 ml of sulphuric acid (3.5), shake to impregnate the substance completely with acid and to prevent it from sticking to the sides of the flask, heat and keep at boiling point for 10 minutes.
Vzorec se prenese v Kjeldahlovo bučko, doda se 20 ml žveplove kisline (3.5), pretrese se, da se vzorec popolnoma prepoji s kislino in da se ne sprijema sten bučke, nato se segreje in pusti vreti 10 minut.
Leave to cool slightly, add 2 ml of nitric acid (3.4), heat gently, leave to cool slightly, add a little more nitric acid (3.4) and bring back to boiling point.
Pusti se, da se nekoliko ohladi, dodata se 2 ml dušikove kisline (3.4), zmerno se segreje, pusti se, da se nekoliko ohladi, doda se še nekaj dušikove kisline (3.4) in ponovno segreje do vretja.
Take 10 ml of each of these solutions and add thereto 10 ml of molybdovanadate reagent (3.6).
V 10 ml vsake izmed teh raztopin se doda 10 ml molibdovanadatnega reagenta (3.6).
Light petroleum layer
Plast petroletra
Mix 500 ml of acetonitrile (3.1) with 300 ml of ammonium acetate buffer solution (3.18) and 1200 ml of water (3.14). Adjust the pH to 4,3 using acetic acid (3.5).
500 ml acetonitrila (3.1) se zmeša s 300 ml pufrske raztopine amonijevega acetata (3.18) in 1200 ml vode (3.14). pH se uravna na 4,3 z ocetno kislino (3.5).
A collaborative study was arranged in which three samples were analysed by eight laboratories.
Organizirana je bila medlaboratorijska študija, v kateri je osem laboratorijev analiziralo tri vzorce.
Stopper and shake for approximately 20 minutes.
Zamaši se in stresa približno 20 minut.
Store in a well stoppered container.
Hrani se v dobro zaprti posodi.
Add 7,5 g molecular sieve (3.4), stopper and shake for five minutes.
Doda se 7,5 g molekularnega sita (3.4), zamaši se in stresa pet minut.
Filter immediately through a glass-fibre filter paper.
Takoj se filtrira skozi filtrirni papir iz steklenih vlaken.
In the first flask place 10 ml of solvent B (3.3), and in the others 2,0, 4,0, 6,0, 8,0 and 10,0 ml of standard gossypol solution B (3.6) respectively.
V prvo bučko se prenese 10 ml topila B (3.3), v druge pa 2,0, 4,0, 6,0, 8,0 in 10,0 ml standardne raztopine gosipola B (3.6).
Make the contents of each flask up to 10 ml with solvent B (3.3).
Vsaka bučka se dopolni do 10 ml s topilom B (3.3).
High sensitivity and low limits of detection.
visoka občutljivost in nizke meje zaznavnosti.
Add 100,0 ml of methanesulfonic acid solution (3.4) and shake mechanically (4.1) for 30 minutes.
Doda se 100,0 ml raztopine metansulfonske kisline (3.4) in mehansko stresa (4.1) 30 minut.
Filter the solution through a filter paper and retain the filtrate for the liquid-liquid partition step (5.3).
Raztopina se filtrira skozi papirni filter, filtrat pa se shrani za postopek ločevanja tekočina-tekočina (5.3).
Transfer the eluate obtained in (5.4.2) into a 1 litre separating funnel.
Eluat, ki nastane pri postopku iz točke 5.4.2, se prenese v litrski lij ločnik.
Rinse the conical flask with 5 to 10 ml methanol (3.2) and combine the washings with the contents of the separating funnel.
Erlenmajerica se spere s 5–10 ml metanola (3.2), izpirek pa se združi z vsebino v liju ločniku.
Observation) Proceed to the HPLC determination (5.3).
Nadaljuje se z določanjem s HPLC (5.3).
Place the flask in the ultrasonic bath (4.4) and leave for 15 minutes.
Erlenmajerica se postavi v ultrazvočno kopel (4.4) in se pusti 15 minut.
Remove the flask from the ultrasonic bath and shake it for 1 h on the shaker or stir on the magnetic stirrer (4.5).
Odstrani se iz ultrazvočne kopeli in 1 uro stresa s stresalnikom ali meša z magnetnim mešalom (4.5).
In the case of products with a high lactose content, such as powdered milk serum or skimmed milk powder, proceed as follows after adding 80 ml of ethanol (3.5). Fit a reflux condenser to the flask and immerse the latter in a water bath at 50 oC for 30 minutes.
V primeru proizvodov, ki imajo visoko vsebnost laktoze, kot je serum mleka v prahu ali posneto mleko v prahu, se po dodatku 80 ml etanola (3.5) izvaja naslednje: bučka se spoji s kondenzatorjem povratnega toka in se za 30 minut potopi v vodno kopel pri 50 oC.
Aqueous layer
Vodna plast
Dissolve 1,36 g of KH2PO4 (3.1) in 500 ml of water (3.11), add 3,5 ml of orthophosphoric acid (3.2) and 10,0 ml of 6-methyl-2-heptylamine (3.4). Adjust the pH to 4,0 with orthophosphoric acid solution (3.3) and dilute to 1000 ml with water (3.11).
1,36 g KH2PO4 (3.1) se raztopi v 500 ml vode (3.11), doda se 3,5 ml ortofosforne kisline (3.2) in 10,0 ml 6-metil-2-heptilamina (3.4). pH se uravna na 4,0 z raztopino ortofosforne kisline (3.3) in razredči z vodo (3.11) do 1000 ml.