natgillin/translations-raw huggingface dataset:
The peak width, at half of its height, must be within ± 10 % of the original width of the olaquindox peak of the unfortified sample extract.
Širina vrha na polovici maksimalne višine mora biti znotraj ± 10 % prvotne širine vrha, ki nastane pri ekstraktu vzorca brez dodanega olakvindoksa.
Diode array detection
Določanje s serijo diod
For diode-array detection this is typically within ± 2 nm.
Pri določanju s serijo diod je to ponavadi ± 2 nm;
Between 220 and 400 nm, the sample and standard spectra recorded at the peak apex of the chromatogram, must not be different for those parts of the spectrum within the range 10 %-100 % of relative absorbance.
med 220 in 400 nm se spekter vzorca in spekter standarda, zabeležena na najvišji točki vrha na kromatogramu, za navedene dele spektra ne smeta razlikovati v območju 10–100 % relativne absorbance.
Between 220 and 400 nm, the spectra of the upslope, apex and downslope of the peak produced by the sample extract must not be different from each other for those parts of the spectrum within the range 10 %-100 % of relative absorbance.
med 220 in 400 nm se spektri naraščanja, najvišje točke vrha in padanja vrha, pridobljeni iz ekstrakta vzorca, za navedene dele spektra ne smejo razlikovati med seboj v območju 10–100 % relativne absorbance.
The difference between the results of two parallel determinations carried out on the same sample must not exceed 15 % relative to the higher result for olaquindox contents between 10 and 200 mg/kg.
Razlika med rezultatoma dveh vzporednih določanj, izvedenih na istem vzorcu, ne sme presegati 15 % višjega rezultata za vsebnosti olakvindoksa 10–200 mg/kg.
An EC collaborative study was arranged in which four piglet feed samples including one blank feed were analysed by up to 13 laboratories.
Organizirana je bila medlaboratorijska študija, pri kateri je 13 laboratorijev analiziralo 4 vzorce krme v briketih, vključno z enim slepim vzorcem.
The results are given below:
Rezultati so prikazani v spodnji preglednici:
Nominal content
Nominalna vsebnost
recovery %
Izkoristek %
Although the method has not been validated for feeds containing more than 100 mg/kg of olaquindox, it may be possible to obtain satisfactory results by taking a smaller sample weight and/or diluting the extract (5.2) to reach a concentration within the range of the calibration graph (5.3.2).
Čeprav metoda ni potrjena za krmo, ki vsebuje več kot 100 mg/kg olakvindoksa, je mogoče dobiti zadovoljive rezultate tako, da se uporabi manjša masa vzorca in/ali razredčitev ekstrakta (5.2), da se doseže koncentracija v območju umeritvene krivulje (5.3.2).
DETERMINATION OF AMPROLIUM
DOLOČANJE AMPROLIUMA
1-[(4-amino-2-propylpyrimidin-5-yl)methyl]-2-methyl-pyridinium chloride hydrochloride
1-[(4-amino-2-propilpirimidin-5-il)metil]-2-metil-piridin klorid hidroklorid
This method makes it possible to determine the level of amprolium in feed and premixtures.
Ta metoda omogoča določanje ravni amproliuma v krmi in premiksih.
The detection limit is 1 mg/kg, the limit of quantification is 5 mg/kg.
Meja določanja je 1 mg/kg, meja kvantifikacije pa 5 mg/kg.
The sample is extracted with a methanol-water mixture.
Vzorec se ekstrahira z mešanico metanola in vode.
After dilution with the mobile phase and membrane filtration the content of amprolium is determined by cation exchange high performance liquid chromatography (HPLC) using a UV detector.
Po redčenju z mobilno fazo in membranski filtraciji se vsebnost amproliuma določi s kationsko izmenjevalno tekočinsko kromatografijo visoke ločljivosti (HPLC) z uporabo UV-detektorja.
Sodium dihydrogen phosphate solution, c = 0,1 mol/l.
Raztopina natrijevega dihidrogen fosfata, c = 0,1 mol/l
Dissolve 13,80 g of sodium dihydrogen phosphate monohydrate in water (3.3) in a 1000 ml graduated flask, make up to the mark with water (3.3) and mix.
V 1000-mililitrski merilni bučki se v vodi (3.3) raztopi 13,80 g natrijevega dihidrogen fosfat monohidrata, dopolni se z vodo (3.3) do oznake in premeša.
Sodium perchlorate solution, c = 1,6 mol/l.
Raztopina natrijevega perklorata, c = 1,6 mol/l
Dissolve 224,74 g of sodium perchlorate monohydrate in water (3.3) in a 1000 ml graduated flask, make up to the mark with water (3.3) and mix.
V 1000-mililitrski merilni bučki se v vodi (3.3) raztopi 224,74 g natrijevega perklorat monohidrata, dopolni se z vodo (3.3) do oznake in premeša.
Mobile phase for HPLC (see observation 9.1).
Mobilna faza za HPLC (glej opombo 9.1).
Mixture of acetonitrile (3.2), sodium dihydrogen phosphate solution (3.4) and sodium perchlorate solution (3.5), 450+450+100 (v+v+v).
Mešanica acetonitrila (3.2), raztopine natrijevega dihidrogen fosfata (3.4) in raztopine natrijevega perklorata (3.5), 450+450+100 (v+v+v)
Prior to use filter through a 0,22 μm membrane filter (4.3) and degas the solution (e.g. in the ultrasonic bath (4.4) for at least 15 minutes).
Pred uporabo se filtrira z membranskim filtrom 0,22 μm (4.3), raztopina pa se razplini (npr. najmanj 15 minut v ultrazvočni kopeli (4.4)).
Standard substance: pure amprolium, 1-[(4-amino-2-propylpyrimidin-5-yl)methyl]-2-methyl-pyridinium chloride hydrochloride, E 750 (see 9.2).
Standardna snov: čisti amprolium, l-[(4-amino-2-propilpirimidin-5-il)metil]-2-metil-piridin klorid hidroklorid, E 750 (glej opombo 9.2)
Amprolium stock standard solution, 500 μg/ml
Osnovna standardna raztopina amproliuma, 500 μg/ml
Weigh to the nearest 0,1 mg, 50 mg of amprolium (3.7) in a 100 ml graduated flask, dissolve in 80 ml methanol (3.1) and place the flask for 10 min. in an ultrasonic bath (4.4).
50 mg amproliuma (3.7) se natehta na 0,1 mg natančno in prenese v 100-mililitrsko merilno bučko ter raztopi v 80 ml metanola (3.1), bučka pa se za 10 minut postavi v ultrazvočno kopel (4.4).
Amprolium intermediate standard solution, 50 μg/ml
Vmesna standardna raztopina amproliuma, 50 μg/ml
Pipette 5,0 ml of the stock standard solution (3.7.1) into a 50 ml graduated flask, make up to the mark with the extraction solvent (3.8) and mix.
5,0 ml osnovne standardne raztopine (3.7.1) se odpipetira v 50-mililitrsko merilno bučko, dopolni se z ekstrakcijskim topilom (3.8) do oznake in premeša.
Transfer 0,5, 1,0 and 2,0 ml of the intermediate standard solution (3.7.2) into a series of 50 ml graduated flasks.
V serijo 50-mililitrskih merilnih bučk se prenese 0,5, 1,0, in 2,0 ml vmesne standardne raztopine (3.7.2).
Make up to the mark with the mobile phase (3.6) and mix.
Dopolni se z mobilno fazo (3.6) do oznake in premeša.
These solutions correspond to 0,5, 1,0 and 2,0 μg of amprolium per ml respectively.
Te raztopine ustrezajo 0,5, 1,0 in 2,0 μg amproliuma na ml.
Extraction solvent.
Ekstrakcijsko topilo
Methanol (3.1)-water mixture 2+1 (v+v).
Mešanica metanola (3.1) in vode 2+1 (v+v)
HPLC equipment with injection system, suitable for injection volumes of 100 μl.
Oprema za HPLC s sistemom za vbrizgavanje, primernim za vbrizgavanje volumnov 100 μl
Liquid chromatographic column 125 mm × 4 mm, cation exchange Nucleosil 10 SA, 5 or 10 μm packing, or equivalent.
Kolona za tekočinsko kromatografijo, 125 mm × 4 mm, kationski izmenjevalec Nucleosil 10 SA, polnitev 5 ali 10 μm ali enakovredna
UV detector with variable wavelength adjustment or diode array detector.
UV-detektor z nastavljivo valovno dolžino ali detektor s serijo diod
Membrane filter, PTFE material, 0,45 μm.
Membranski filter, material PTFE, 0,45 μm
Membrane filter, 0,22 μm.
Membranski filter, 0,22 μm
Ultrasonic bath.
Ultrazvočna kopel
For the performance of the recovery test (5.1.2) a blank feed shall be analysed to check that neither amprolium nor interfering substances are present.
Za učinkovitost preskusa izkoristka (5.1.2) se z analizo slepe krme zagotovi, da niso prisotni niti amprolium niti moteče snovi.
The blank feed shall be similar in type to that of the sample and amprolium or interfering substances must not be detected.
Vrsta slepe krme je podobna vrsti vzorca in dokaže se odsotnost amproliuma ali motečih snovi.
A recovery test shall be carried out by analysing the blank feed which has been fortified by addition of a quantity of amprolium, similar to that present in the sample.
Preskus izkoristka se izvede z analizo slepe krme, ki se ji doda količina amproliuma, podobna količini v vzorcu.
To fortify at a level of 100 mg/kg, transfer 10,0 ml of the stock standard solution (3.7.1) to a 250 ml conical flask and evaporate the solution to approximately 0,5 ml.
Za koncentracijo 100 mg/kg se 10,0 ml osnovne standardne raztopine (3.7.1) prenese v 250-mililitrsko erlenmajerico, raztopina pa se upari do približno 0,5 ml.
In this case, the sample to be analysed is fortified with a quantity of amprolium similar to that already present in the sample.
V tem primeru se vzorcu za analizo doda toliko amproliuma, kolikor ga je v vzorcu.
This sample is analysed together with the unfortified sample and the recovery can be calculated by subtraction.
Ta vzorec se analizira skupaj z vzorcem brez dodanega amproliuma, izkoristek pa se izračuna z odštevanjem.
Premixtures (content < 1 % amprolium) and feed
Premiksi (vsebnost amproliuma < 1 %) in krma
Weigh to the nearest 0,01 g, 5-40 g of the sample depending on the amprolium content into a 500 ml conical flask and add 200 ml extraction solvent (3.8).
V 500-mililitrsko erlenmajerico se natehta na 0,01 g natančno 5–40 g vzorca, odvisno od vsebnosti amproliuma, in doda 200 ml ekstrakcijskega topila (3.8).
Dilute an aliquot of the extract with the mobile phase (3.6) to an amprolium content of 0,5-2 μg/ml and mix (see observation 9.3).
Alikvot ekstrakta se razredči z mobilno fazo (3.6), da nastane vsebnost amproliuma 0,5–2 μg/ml, ter premeša (glej opombo 9.3).
Filter 5-10 ml of this diluted solution on a membrane filter (4.2).
Odstrani se iz ultrazvočne kopeli in 1 uro stresa s stresalnikom ali meša z magnetnim mešalom (4.5). Alikvot ekstrakta se razredči z mobilno fazo (3.6), da nastane vsebnost amproliuma 0,5–2 μg/ml, ter premeša. 5–10 ml tako razredčene raztopine se filtrira skozi membranski filter (4.2).
Premixtures (content ≥ 1 % amprolium)
Premiksi (vsebnost amproliuma ≥ 1 %)
Liquid chromatographic
Kolona za tekočinsko
column (4.1.1):
kromatografijo (4.1.1):
125 mm × 4 mm, cation exchange Nucleosil 10 SA, 5 or 10 μm packing, or equivalent
125 mm × 4 mm, kationski izmenjevalec Nucleosil 10 SA, polnitev 5 ali 10 μm ali enakovredna
Mobile phase (3.6):
Mobilna faza (3.6):
Check the stability of the chromatographic system, injecting several times the calibration solution (3.7.3) containing 1,0 μg/ml, until constant peak heights and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizgavanjem raztopine za umerjanje (3.7.3) s koncentracijo 1,0 μg/ml, dokler se ne dosežejo konstantne višine vrhov in konstantni retencijski časi.
Inject each calibration solution (3.7.3) several times and determine the mean peak heights (areas) for each concentration.
Za vsako koncentracijo se vsaka raztopina za umerjanje (3.7.3) vbrizga večkrat, nato pa se določijo srednje vrednosti višin (površin) vrhov.
Inject the sample extract (5.2) several times using the same volume as taken for the calibration solutions and determine the mean peak height (area) of the amprolium peaks.
Ekstrakt vzorca (5.2) se večkrat vbrizga, pri čemer se uporabijo enaki volumni kakor pri raztopini za umerjanje, nato pa se določijo srednje vrednosti višin (površin) vrhov amproliuma.
From the mean height (area) of the amprolium peaks of the sample solution determine the concentration of the sample solution in μg/ml by reference to the calibration graph (5.3.2).
Koncentracija raztopine vzorca v μg/ml se določi iz srednje vrednosti višin (površin) vrhov amproliuma iz umeritvene krivulje (5.3.2).
The amprolium content w in mg/kg of the sample is given by the following formula:
Vsebnost amproliuma (w) v mg/kg vzorca se izračuna po naslednji formuli:
V volume of the extraction solvent (3.8) in ml according to 5.2 (i.e. 200 ml)
V volumen ekstrakcijskega topila (3.8) v ml v skladu s točko 5.2 (tj. 200 ml);
c amprolium concentration of the sample extract (5.2) in μg/ml
c koncentracija amproliuma v ekstraktu vzorca (5.2) v μg/ml;
f dilution factor according to 5.2
f faktor razredčitve v skladu s točko 5.2;
m weight of the test portion in g.
m masa preskusnega vzorca v gramih.
The identity of the analyte can be confirmed by co-chromatography, or by using a diode-array detector by which the spectra of the sample extract (5.2) and the calibration solution (3.7.3) containing 2,0 μg/ml are compared.
Identiteta analita se lahko potrdi z dodatno kromatografijo ali z detektorjem s serijo diod, s katerim se primerjata spekter ekstrakta vzorca (5.2) in spekter raztopine za umerjanje (3.7.3) s koncentracijo 2,0 μg/ml.
A sample extract (5.2) is fortified by addition of an appropriate amount of calibration solution (3.7.3).
Ekstraktu vzorca (5.2) se doda primerna količina raztopine za umerjanje (3.7.3).
The amount of added amprolium must be similar to the amount of amprolium found in the sample extract.
Količina dodanega amproliuma mora biti podobna količini amproliuma v ekstraktu vzorca.
Only the height of the amprolium peak shall be enhanced after taking into account both the amount added and the dilution of the extract.
Povišan je le vrh amproliuma ob upoštevanju dodane količine in razredčitve ekstrakta.
The peak width, at half of its height, must be within ± 10 % of the original width of the amprolium peak of the unfortified sample extract.
Širina vrha na polovici maksimalne višine mora biti znotraj ± 10 % prvotne širine vrha, ki nastane pri ekstraktu vzorca brez dodanega amproliuma.
Between 210 and 320 nm, the sample and standard spectra recorded at the peak apex of the chromatogram, must not be different for those parts of the spectrum within the range 10 %-100 % of relative absorbance.
med 210 in 320 nm se spekter vzorca in spekter standarda, zabeležena na najvišji točki vrha na kromatogramu, za navedene dele spektra ne smeta razlikovati v območju 10–100 % relativne absorbance.
Between 210 and 320 nm, the spectra of the upslope, apex and downslope of the peak produced by the sample extract must not be different from each other for those parts of the spectrum within the range 10 %-100 % of relative absorbance.
med 210 in 320 nm se spektri naraščanja, najvišje točke vrha in padanja vrha, pridobljeni iz ekstrakta vzorca, za navedene dele spektra ne smejo razlikovati med seboj v območju 10–100 % relativne absorbance.
If one of these criteria is not met, the presence of the analyte has not been confirmed.
Če eno izmed teh meril ni izpolnjeno, prisotnost analita ni potrjena.
15 % relative to the higher value for amprolium contents from 25 mg/kg to 500 mg/kg,
15 % višje vrednosti za vsebnosti amproliuma 25–500 mg/kg,
75 mg/kg for amprolium contents between 500 mg/kg and 1000 mg/kg,
75 mg/kg za vsebnosti amproliuma 500–1000 mg/kg,
7,5 % relative to the higher value for amprolium contents of more than 1000 mg/kg.
7,5 % višje vrednosti za vsebnosti amproliuma nad 1000 mg/kg.
For a fortified (blank) sample the recovery shall be at least 90 %.
Pri vzorcu z dodatkom (slepem vzorcu) je izkoristek najmanj 90-odstoten.
A collaborative study was arranged in which three poultry feeds (sample 1-3), one mineral feed (sample 4) and one premix (sample 5) were analysed.
Organizirana je bila medlaboratorijska študija, pri kateri so bili analizirani trije vzorci perutninske krme (vzorci 1–3), en vzorec mineralne krme (vzorec 4) in en vzorec premiksa (vzorec 5).
Sample 1 (blank feed)
Vzorec 1 (slepa krma)
nominal content [mg/kg]
Nominalna vsebnost [mg/kg]
If the sample contains thiamine, the thiamine peak in the chromatogram appears shortly before the amprolium peak.
Če vzorec vsebuje tiamin, se vrh tiamina v kromatogramu pojavi malo pred vrhom amproliuma.
Following this method amprolium and thiamine must be separated.
Pri tej metodi se morata amprolium in tiamin ločiti.
If the amprolium and thiamine are not separated by the column (4.1.1) used in this method, replace up to 50 % of the acetonitrile portion of the mobile phase (3.6) by methanol.
Če se amprolium in tiamin ne ločita s kolono (4.1.1), ki se uporablja pri tej metodi, se do 50 % acetonitrila v mobilni fazi (3.6) zamenja z metanolom.
According to the British Pharmacopoeia, the spectrum of an amprolium solution (c = 0,02 mol/l) in hydrochloric acid (c = 0,1 mol/l) shows maxima at 246 nm and 262 nm.
Po britanski farmakopeji sta najvišji točki spektra raztopine amproliuma (c = 0,02 mol/l) v klorovodikovi kislini (c = 0,1 mol/l) pri 246 nm in 262 nm.
The absorbance shall amount to 0,84 at 246 nm and 0,80 at 262 nm.
Absorbanca znaša 0,84 pri 246 nm in 0,80 pri 262 nm.
The extract must always be diluted with the mobile phase, because otherwise the retention time of the amprolium peak may shift significantly, due to changes in the ionic strength.
Ekstrakt se mora vedno razredčiti z mobilno fazo, ker se sicer retencijski čas vrha amproliuma bistveno premakne zaradi sprememb v ionski moči.
DETERMINATION OF CARBADOX
DOLOČANJE KARBADOKSA
Methyl 3-(2-quinoxalinylmethylene)carbazate N1,N4-dioxide
Metil 3-(2-kvinoksalinilmetilen)karbazat N1,N4-dioksid
This method makes it possible to determine the level of carbadox in feed, premixtures and preparations.
Ta metoda omogoča določanje ravni karbadoksa v krmi, premiksih in pripravkih.
The detection limit is 1 mg/kg. The limit of quantification is 5 mg/kg.
Meja določanja je 1 mg/kg, meja kvantifikacije pa 5 mg/kg.
The sample is equilibrated with water and extracted with methanol-acetonitrile.
Vzorec se uravnoteži z vodo in ekstrahira z metanol-acetonitrilom.
For feed, an aliquot portion of the filtered extract is subjected to clean-up on an aluminium oxide column.
Pri krmi se alikvot filtriranega ekstrakta prečisti na koloni iz aluminijevega oksida.
For premixtures and preparations an aliquot portion of the filtered extract is diluted to an appropriate concentration with water, methanol and acetonitrile.
Pri premiksih in pripravkih se alikvot filtriranega ekstrakta z vodo, metanolom in acetonitrilom razredči do primerne koncentracije.
The content of carbadox is determined by reversed-phase high-performance liquid chromatography (HPLC) using a UV detector.
Vsebnost karbadoksa se določi s tekočinsko kromatografijo visoke ločljivosti (HPLC) z reverzno fazo z uporabo UV-detektorja.
Acetic acid, w = 100 %.
Ocetna kislina, m = 100 %
Aluminium oxide: neutral, activity grade I.
Aluminijev oksid: nevtralni, stopnja aktivnosti I
Mix 500 ml of methanol (3.1) with 500 ml of acetonitrile (3.2).
500 ml metanola (3.1) se zmeša s 500 ml acetonitrila (3.2).
Acetic acid, σ = 10 %.
Ocetna kislina, σ = 10 %
Dilute 10 ml acetic acid (3.3) to 100 ml with water.
10 ml ocetne kisline (3.3) se razredči z vodo do 100 ml.
Sodium acetate.
Natrijev acetat
Acetate buffer solution, c = 0,01 mol/l, pH = 6,0.
Raztopina acetatnega pufra, c = 0,01 mol/l, pH = 6,0
Dissolve 0,82 g of sodium acetate (3.7) in 700 ml of water (3.8) and adjust the pH to 6,0 with acetic acid (3.6).
0,82 g natrijevega acetata (3.7) se raztopi v 700 ml vode (3.8), pH pa se z ocetno kislino (3.6) uravna na 6,0.
Transfer to a 1000 ml graduated flask, make up to the mark with water (3.8) and mix.
Prenese se v 1000-mililitrsko merilno bučko, dopolni se z vodo (3.8) do oznake in premeša.
Mix 825 ml of acetate buffer solution (3.9) with 175 ml of acetonitrile (3.2).
825 ml raztopine acetatnega pufra (3.9) se zmeša s 175 ml acetonitrila (3.2).
Pure carbadox: Methyl 3-(2-quinoxalinylmethylene)carbazate N1,N4-dioxide, E 850.
Čisti karbadoks: metil 3-(2-kvinoksalinilmetilen)karbazat N1,N4-dioksid, E 850
Carbadox stock standard solution, 100 μg/ml (see Note 5. Procedure):
Osnovna standardna raztopina karbadoksa, 100 μg/ml (glej točko 5 Postopek):
Weigh to the nearest 0,1 mg, 25 mg of carbadox standard substance (3.11) into a 250 ml graduated flask.
25 mg standardne snovi karbadoksa (3.11) se natehta na 0,1 mg natančno in prenese v 250-mililitrsko merilno bučko.
Dissolve in methanol-acetonitrile (3.5) by ultrasonification (4.7).
V ultrazvočni kopeli (4.7) se raztopi v metanol-acetonitrilu (3.5).
After ultrasonic treatment bring the solution to room temperature, make up to the mark with methanol-acetonitrile (3.5) and mix.
Po ultrazvočni obdelavi se raztopina segreje na sobno temperaturo, dopolni se z metanol-acetonitrilom (3.5) do oznake in premeša.
Wrap the flask with aluminium foil or use amber glassware and store in a refrigerator.
Bučka se ovije v aluminijasto folijo ali pa se uporabi rjava bučka ter shrani v hladilniku.
Transfer 2,0, 5,0, 10,0, and 20,0 ml of the stock standard solution (3.11.1) into a series of 100 ml calibrated flasks.
2,0, 5,0, 10,0 in 20,0 ml osnovne standardne raztopine (3.11.1) se prenese v serijo 100-mililitrskih merilnih bučk.
Add 30 ml of water, make up to the mark with methanol-acetonitrile (3.5) and mix.
Doda se 30 ml vode, dopolni se z metanol-acetonitrilom (3.5) do oznake in premeša.
These solutions correspond to 2,0, 5,0, 10,0 and 20,0 μg/ml of carbadox respectively.
Te raztopine ustrezajo koncentracijam karbadoksa 2,0, 5,0, 10,0, in 20,0 μg/ml.
Calibration solutions must be freshly prepared before use.
Raztopine za umerjanje je treba pripraviti sveže pred uporabo.
For the determination of carbadox in feed containing less than 10 mg/kg, calibration solutions with a concentration below 2,0 μg/ml must be prepared.
Za določanje karbadoksa v krmi, ki vsebuje manj kot 10 mg/kg karbadoksa, se morajo pripraviti raztopine za umerjanje s koncentracijo, nižjo od 2,0 μg/ml.
Water-[methanol-acetonitrile] (3.5) mixture, 300 + 700 (v + v).
Mešanica voda-[metanol-acetonitril] (3.5), 300 + 700 (v + v)
Mix 300 ml of water with 700 ml of the mixture of methanol-acetonitrile (3.5).
300 ml vode se zmeša s 700 ml mešanice metanola in acetonitrila (3.5).
Laboratory shaker or magnetic stirrer.
Laboratorijski stresalnik ali magnetno mešalo
Glass column (length 300 to 400 mm, internal diameter approximately 10 mm) with sintered glass frit and draw-off valve.
Steklena kolona (dolžina 300 do 400 mm, notranji premer približno 10 mm) s sintrano stekleno frito in odtočnim ventilom
a glass column fitted with a stopcock or a glass column with a tapered end may also be used; in this case, a small glass-wool plug is inserted into the lower end and it is tamped down using a glass rod.
Lahko se uporablja tudi steklena kolona s petelinčkom ali zamaškom; v tem primeru se v spodnji konec vstavi majhen zamašek iz steklene volne in se potlači s stekleno palčko.
HPLC equipment with injection system, suitable for injection volumes of 20 μl.
Oprema za HPLC s sistemom za vbrizgavanje, primernim za vbrizgavanje volumnov 20 μl
Liquid chromatographic column: 300 mm x 4 mm, C18, 10 μm packing or equivalent.
Kolona za tekočinsko kromatografijo: 300 mm x 4 mm, C18, polnitev 10 μm ali enakovredna
UV detector with variable wavelength adjustment or diode array detector operating in the range of 225 to 400 nm.
UV-detektor z nastavljivo valovno dolžino ali detektor s serijo diod v območju 225 do 400 nm
Carbadox is light-sensitive.
Karbadoks je občutljiv na svetlobo.
Carry out all procedures under subdued light or use amber glassware or glassware wrapped in aluminium foil.
Vsi postopki se izvajajo pri zmanjšani svetlobi ali pa se uporabi posoda iz rjavega stekla ali se bučka ovije v aluminijasto folijo.
For the performance of the recovery test (5.1.2) a blank feed shall be analysed to check that neither carbadox nor interfering substances are present.
Za učinkovitost preskusa izkoristka (5.1.2) se z analizo slepe krme zagotovi odsotnost karbadoksa in motečih snovi.
The blank feed shall be similar in type to that of the sample and on analysis carbadox or interfering substances must not be detected.
Vrsta slepe krme je podobna vrsti vzorca in pri analizi se dokaže odsotnost karbadoksa ali motečih snovi.
A recovery test shall be carried out by analysing the blank feed (5.1.1) which has been fortified by the addition of a quantity of carbadox, similar to that present in the sample.
Preskus izkoristka se izvede z analizo slepe krme (5.1.1) z dodatkom količine karbadoksa, podobne količini v vzorcu.