natgillin/translations-raw huggingface dataset:
Colouring with staining reagents (6.3) can additionally be applied for the identification.
Za identifikacijo se dodatno lahko uporabi barvanje z reagenti za barvanje (6.3).
In order to indicate the nature of the animal proteins and the origin of the particles, a decision support system such as ARIES can be used and reference samples can be documented.
Za opredelitev narave živalskih beljakovin in izvora delcev se lahko uporabi sistem podpore pri določanju, kot je ARIES, in dokumentirajo referenčni vzorci.
Identification of constituents of animal origin in the sieve fractions
Identifikacija sestavin živalskega izvora v presejanih frakcijah
At least 5 g of the sample is sieved through the sieve (5.3) in two fractions
Najmanj 5 g vzorca se preseje skozi sito (5.3) v dve frakciji.
The sieve fraction(s) with the large particles (or a representative part of the fraction) is applied as a thin layer to a suitable support and screened systematically under the stereomicroscope (5.5) at various magnifications for constituents of animal origin.
Presejane frakcije z velikimi delci (ali reprezentativni del frakcije) se v tankem sloju nanesejo na primerno podlago ter se pri različnih povečavah sistematično pregledajo pod stereomikroskopom (5.5), da se ugotovijo sestavine živalskega izvora.
Slides made with the sieve fraction(s) with the fine particles are screened systematically under the compound microscope (5.6) at various magnifications for constituents of animal origin.
Mikroskopski preparati iz presejanih frakcij z drobnimi delci se sistematično pregledajo pod sestavljenim mikroskopom (5.6) pri različnih povečavah, da se ugotovijo sestavine živalskega izvora.
Identification of constituents of animal origin from the concentrated sediment
Opredelitev sestavin živalskega izvora v koncentrirani usedlini
At least 5 g (accurate to 0,01 g) of the sample shall be transferred into a separation funnel or conical bottomed settling beaker and treated with at least 50 ml of tetrachloroethylene (4.3.1).
V lij ločnik ali konično čašo za usedlino se prenese najmanj 5 g (na 0,01 g natančno) vzorca, ki se obdela z najmanj 50 ml tetrakloroetilena (4.3.1).
The mixture shall be shaken or stirred repeatedly.
Mešanica se večkrat pretrese ali premeša.
If a closed separation funnel is used the sediment shall be left to stand for a sufficient time (at least 3 minutes) before the sediment is separated off. Shaking shall be repeated and the sediment shall be left to stand again at least 3 minutes.
Če se uporabi zaprt lij ločnik, se usedlina pusti stati tako dolgo (najmanj 3 minute), dokler se ne loči. Usedlina se ponovno pretrese in ponovno pusti stati najmanj 3 minute.
The sediment shall be separated off again.
Usedlina se ponovno loči.
If an open beaker is used, the sediment shall be left to stand for at least 5 minutes before the sediment is separated off.
Če se uporabi odprta čaša, se usedlina pusti stati vsaj 5 minut, dokler se ne loči.
The total sediment shall be dried and subsequently weighed (accurate to 0,001 g).
Celotna usedlina se osuši in nato stehta (na 0,001 g natančno).
The weighing is only necessary in case an estimation is required.
Tehtanje je potrebno le, če se zahteva ocena.
If the sediment consists of many large particles it may be sieved through a sieve (5.3) in two fractions.
Če usedlina vsebuje mnogo velikih delcev, se lahko skozi sito (5.3) preseje v dve frakciji.
The dried sediment shall be examined for bone constituents under the stereomicroscope (5.5) and the compound microscope (5.6).
Posušena usedlina se pregleda pod stereomikroskopom (5.5) in sestavljenim mikroskopom (5.6), da se ugotovijo kostne sestavine.
Use of embedding agents and staining reagents.
Uporaba sredstev za pripravo preparatov in reagentov za obarvanje
The microscopic identification of the constituents of animal origin can be supported by the use of special embedding agents and staining reagents.
Mikroskopska identifikacija sestavin živalskega izvora se lahko izboljša tudi s pomočjo posebnih sredstev za pripravo preparatov in reagentov za obarvanje.
Chloral hydrate (4.1.1): By carefully heating, cell structures can be seen more clearly because starch grains gelatinise and unwanted cell contents are removed.
Kloralhidrat (4.1.1): Ob previdnem segrevanju so lahko celične strukture jasnejše vidne, ker se škrobne verige želatinizirajo, nezaželene celične sestavine pa odstranijo.
Lye (4.1.2): either sodiumhydroxide or potassiumhydroxide clears the material of the feed, assisting the detection of muscle fibres, hairs and other keratin structures.
Lug (4.1.2): krmni material se očisti z natrijevim hidroksidom ali kalijevim hidroksidom, kar olajša določanje mišičnih vlaken, ščetin in drugih keratinskih struktur.
Paraffin oil and glycerol (4.1.3): Bone constituents can be well identified in this embedding agent because most lacunae remain filled with air and appear as black holes about 5-15 μm .
Parafinsko olje in glicerol (4.1.3): Kostne sestavine se lahko dobro identificirajo v tem sredstvu za pripravo preparatov, ker je v večini kostnih votlinic (lacunae) še vedno zrak in so zato vidne kot črne luknje, velikosti približno 5–15 μm.
Iodine/potassium Iodide solution (4.4.1): Is used for the detection of starch (Blue-violet colour) and protein (yellow-orange colour).
Raztopina joda/kalijevega jodida (4.4.1): uporablja se za določanje škroba (modrovijolična barva) in beljakovin (rumenooranžna barva).
Solutions may be diluted if required.
Raztopine se lahko po potrebi razredčijo.
Alizarin red solution (4.4.2): Red/pink colouring of bones, fish-bones and scales.
Raztopina rdečega alizarina (4.4.2): rdečerožnato obarvanje kosti, ribjih kosti in luskin.
Before drying the sediment (see section 6.2), the total sediment shall be transferred into a glass test tube and rinsed twice with approximately 5 ml alcohol (4.2.1) (each time a vortex shall be used, the solvent shall be let settle about one minute and poured off).
Pred sušenjem sedimenta (glej točko 6.2) se celotni sediment prenese v stekleno epruveto in dvakrat spere s približno 5 ml alkohola (4.2.1) (pri vsaki uporabi vorteks mešalnika se topilo pusti stati približno 1 minuto ter se nato odtoči).
Before using this staining reagent, the sediment shall be bleached by adding at least 1 ml sodium hypochlorite solution (4.5.1).
Pred uporabo tega reagenta za obarvanje se sediment pobeli z dodatkom najmanj 1 ml raztopine natrijevega hipoklorita (4.5.1).
The reaction shall be allowed continue during 10 minutes.
Čas reakcije je 10 minut.
The tube shall be filled with water, the sediment shall be let settle 2-3 minutes, and the water and the suspended particles shall be poured off. The sediment shall be rinsed twice more with about 10 ml of water (a vortex shall be used, let settle, and pour off the water each time).
Epruveta se napolni z vodo, usedlina se pusti stati 2–3 minute, voda in raztopljeni delci pa se odtočijo. Usedlina se dvakrat spere z 10 ml vode (uporabi se vorteks mešalnik, pusti se stati in vsakič se odtoči voda).
Two to 10 or more drops (depending on the amount of residue) of the alizarine red solution shall be added.
Doda se 2–10 ali več kapljic (glede na količino ostanka) raztopine rdečega alizarina.
The mixture shall be shaken and the reaction shall be let occur a few seconds.
Mešanica se stresa, reakcija pa poteka nekaj sekund.
The coloured sediment shall be rinsed twice with approximately 5 ml alcohol (4.2.1) followed by one rinse with acetone (4.2.2) (each time a vortex shall be used, the solvent shall be let settle about one minute and poured off).
Obarvana usedlina se dvakrat spere s približno 5 ml alkohola (4.2.1), nato pa enkrat z acetonom (4.2.2) (vsakič ko se uporabi vorteks mešalnik, se topilo pusti stati približno 1 minuto ter se nato odtoči).
The sediment is then ready to be dried.
Usedlina je takrat pripravljena za sušenje.
Cystin reagent: (4.4.3): By carefully heating, cystin-containing constituents (hair, feathers, etc.) become black-brown.
Cistinski reagent (4.4.3): Pri previdnem segrevanju se sestavine, ki vsebujejo cistin (ščetine, perje itd.), obarvajo črnorjavo.
Examination in feed possibly containing fishmeal
Pregled krme zaradi morebitne vsebnosti ribje moke
At least one slide shall be examined from the fine sieve fraction and from the fine fraction of the sediment under the compound microscope (see sections 6.1 and 6.2).
Pod sestavljenim mikroskopom se pregleda najmanj en mikroskopski preparat iz drobno presejane frakcije in drobne frakcije usedline (glej točki 6.1 in 6.2).
Where the label indicates that the ingredients include fishmeal, or if the presence of fishmeal is suspected or detected in the initial examination, at least two additional slides of the fine sieve fraction from the original sample, and the total sediment fraction shall be examined.
Kadar oznaka navaja, da sestavine vsebujejo ribjo moko, ali kadar obstaja sum na vsebnost ribje moke ali kadar se odkrije vsebnost ribje moke že pri začetni preiskavi, se iz izvirnega vzorca pregledata najmanj dva dodatna mikroskopska preparata iz drobno presejane frakcije ter frakcije celotne usedline.
Calculation and evaluation
Izračun in ocena
Member States shall ensure that the procedures described in this point are used where an official analysis is carried out with a view to estimate the amount (and not simply the presence) of animal constituents.
Države članice zagotovijo, da se postopki, opisani v tej točki, uporabljajo pri izvajanju uradne analize, da se oceni količina (in ne zgolj vsebnost) sestavin živalskega izvora.
The calculation can only be made if the constituents of animal origin contain bone fragments.
Izračun je možno narediti le, če sestavine živalskega izvora vsebujejo delce kosti.
Bone fragments of terrestrial warm-blooded species (i.e. mammals and birds) can be distinguished from the different types of fish bone on the microscopic slide by means of the typical lacunae.
Delci kosti toplokrvnih kopenskih vrst (tj. sesalcev in ptic) se lahko v mikroskopskih preparatih ločijo od različnih vrst ribjih kosti na podlagi tipičnih kostnih votlinic (lacunae).
The proportion of constituents of animal origin in the sample material is estimated taking into consideration:
Delež sestavin živalskega izvora se v vzorčenem materialu oceni glede na:
the estimated proportion (weight %) of bone fragments in the concentrated sediment, and
ocenjeni delež (masni %) kostnih delcev v koncentrirani usedlini in
the proportion (weight %) of bone in the constituents of animal origin.
delež (masni %) kosti v sestavinah živalskega izvora.
The estimate has to be based on at least three (if possible) slides and at least five fields per slide.
Ocena mora temeljiti na najmanj treh mikroskopskih preparatih (če je možno) in na najmanj petih poljih na preparat.
In compound feed, the concentrated sediment as a rule contains not only terrestrial animal bone and fish bone fragments, but also other particles of high specific weight, e.g. minerals, sand, lignified plant fragments and the like.
Koncentrirana usedlina v krmnih mešanicah praviloma ne vsebuje samo delcev kosti kopenskih živali in rib, ampak tudi druge delce z visoko specifično maso, npr. minerale, pesek, delce olesenelih rastlin in podobno.
Estimated value of the percentage of bone fragments
Ocenjena vrednost odstotnega deleža delcev kosti
% terrestrial bone fragments = (S × c)/W
% delcev kosti kopenskih živali = (S × c)/W
% fish bone and scale fragments = (S × d)/W
% delcev ribjih kosti in luskin = (S × d)/W
sediment weight (mg), c = correction factor (%) for the estimated portion of terrestrial animal bones in the sediment, d = correction factor (%) for the estimated portion of fish bones and scale fragments in the sediment, W = weight of the sample material for the sedimentation (mg)).
masa usedline (mg), c = korekcijski faktor (%) za ocenjeni delež kosti kopenskih živali v usedlini, d = korekcijski faktor (%) za ocenjeni delež delcev ribjih kosti in luskin v usedlini, W = masa materiala vzorca za sedimentacijo (mg)).
Estimated value of constituents of animal origin
Ocenjena vrednost sestavin živalskega izvora
The proportion of bone in animal products can vary greatly.
Delež kosti se v živalskih proizvodih lahko močno razlikuje.
(The percentage of bone in the case of bone meals is of the order of 50 %-60 % and in the case of meat meals of the order of 20 %-30 %; in case of fish meals bone and scale contents vary according the category and origin of fish meal, normally in the order of 10-20 %).
(Delež kosti pri kostni moki je 50–60 %, pri mesni moki pa 20–30 %; pri ribji moki se vsebnost kosti in luskin razlikuje glede na kategorijo in izvor ribje moke, ponavadi pa je 10–20 %).
If the type of animal meal present in the sample is known, it is possible to estimate the content:
Če je znana vrsta živalske moke, prisotne v vzorcu, je možno oceniti vsebnost:
Estimated content of constituents of terrestrial animal products (%)= (S × c)/(W × f) × 100
ocenjena vsebnost sestavin proizvodov kopenskih živali (%) = (S × c)/(W × f) × 100;
Estimated content of constituents of fish products (%) = (S × d)/(W × f) × 100
ocenjena vsebnost sestavin ribjih proizvodov (%) = (S × d)/(W × f) × 100
sediment weight (mg), c = correction factor (%) for the estimated portion of terrestrial animal bone constituents in the sediment, d = correction factor (%) for the estimated portion of fish bones and scale fragments in the sediment, f = correction factor for the proportion of bone in the constituents of animal origin in the sample examined, W = weight of the sample material for the sedimentation (mg)).
masa usedline (mg), c = korekcijski faktor (%) za ocenjeni delež kostnih sestavin kopenskih živali v usedlini, d = korekcijski faktor (%) za ocenjeni delež delcev ribjih kosti in luskin v usedlini, f = korekcijski faktor za delež kosti v sestavinah živalskega izvora v pregledanem vzorcu, W = masa materiala vzorca za sedimentacijo (mg)).
Expression of the result of the examination
Prikaz rezultatov preiskave
The report shall at least contain information on the presence of constituents derived from terrestrial animals and from fishmeal.
Poročilo vsebuje vsaj informacije o prisotnosti sestavin iz kopenskih živali in ribje moke.
The different cases shall be reported in the following way:
O različnih primerih se poroča na naslednji način:
With regard to the presence of constituents derived from terrestrial animals:
za prisotnost sestavin iz kopenskih živali:
as far as was discernible using a microscope, no constituents derived from terrestrial animals were found in the submitted sample,
kolikor je bilo razvidno iz mikroskopske preiskave, v dostavljenem vzorcu niso bile ugotovljene nobene sestavine iz kopenskih živali
as far as was discernible using a microscope, constituents derived from terrestrial animals were found in the submitted sample.
kolikor je bilo razvidno iz mikroskopske preiskave, so bile v dostavljenem vzorcu ugotovljene sestavine iz kopenskih živali.
and, with regard to the presence of fishmeal:
za prisotnost ribje moke:
as far as was discernible using a microscope, no constituents derived from fish were found in the submitted sample,
kolikor je bilo razvidno iz mikroskopske preiskave, v dostavljenem vzorcu niso bile ugotovljene nobene sestavine ribjega izvora
as far as was discernible using a microscope, constituents derived from fish were found in the submitted sample.
kolikor je bilo razvidno iz mikroskopske preiskave, so bile v dostavljenem vzorcu ugotovljene sestavine ribjega izvora.
In case constituents derived from fish or terrestrial animals are found, the report of the examination result, if required, can further indicate an estimation of the amount of constituents detected (x %, < 0,1 %, 0,1-0,5 %, 0,5-5 % or > 5 %), further specification of the type of terrestrial animal if possible and the animal constituents identified (muscle fibres, cartilage, bones, horn, hair, bristles, feathers, blood, egg shells, fish bones, scales).
V primeru ugotovljenih sestavin ribjega izvora ali izvora kopenskih živali se lahko v poročilu o rezultatih pregleda, če se zahteva, nadalje navede ocena količine ugotovljenih sestavin (x %, < 0,1 %, 0,1–0,5 %, 0,5–5 % ali > 5 %), podrobno opredeli vrsta kopenske živali, če je možno, in ugotovi živalske sestavine (mišična vlakna, hrustanec, kosti, roževina, ščetine, perje, kri, jajčne lupine, ribje kosti, luskine).
For the case where the amount of animal ingredients is estimated the correction factor f used shall be mentioned.
Kadar se ocenjuje količina sestavin živalskega izvora, se navede uporabljeni korekcijski faktor f.
For the cases where bone constituents from terrestrial animals are identified, the report shall contain the additional clause:
Kadar se identificirajo kostne sestavine kopenskih živali, poročilo vsebuje dodatni stavek:
‘The possibility that the above constituents are derived from mammals cannot be excluded.’
„Možnost, da so bile zgornje sestavine pridobljene iz sesalcev, ne more biti izključena.“
This additional clause is not necessary in cases where the bone fragments from terrestrial animals have been specified as bone fragments from poultry or mammals.
Ta dodatni stavek ni potreben, kadar so delci kosti kopenskih živali opredeljeni kot delci kosti perutnine ali sesalcev.
Optional protocol for analysing fat or oil
Izbirni protokol za analizo maščobe ali olja
The following protocol may be used for the analysis of fat or oil:
Za analizo maščobe ali olja se lahko uporabi naslednji protokol:
If the fat is solid, it is warmed for example in a microwave oven until it is liquid.
če je maščoba trdna, se segreva v na primer mikrovalovni pečici, dokler ne postane tekoča,
By using a pipette, 40 ml of fat is transferred from the bottom of the sample to a centrifugation tube.
40 ml maščobe z dna vzorca se odpipetira v centrifugirko,
Centrifugate during 10 minutes at 4000 r.p.m.
centrifugira se 10 minut pri 4000 obr./min.,
If the fat is solid after centrifugation, it is warmed once more in an oven until it is liquid.
če je maščoba trdna po centrifugiranju, se ponovno segreva v pečici, dokler ne postane tekoča.
Repeat the centrifugation during 5 minutes at 4000 r.p.m.
Ponovno se centrifugira 5 minut pri 4000 obr./min.,
By using a small spoon or a spatula one half of the decanted impurities is transferred to a small Petri dish or a microscopic slide for microscopic identification of a possible content of animal constituents (meat fibres, feathers, bone fragments, …).
z majhno žličko ali lopatico se polovica dekantirane nečistoče prenese v majhno petrijevko ali na mikroskopsko stekelce za mikroskopsko identifikacijo morebitne vsebnosti sestavin živalskega izvora (mesnih vlaken, perja, delcev kosti itd.).
As embedding agent for microscopy paraffin oil or glycerol is recommended.
Kot sredstvo za pripravo preparatov za mikroskopijo se priporoča parafinsko olje ali glicerol,
The remaining impurities are used for sedimentation as described in point 6.2.
ostanki nečistoče se uporabijo za usedlino, kakor je opisano v točki 6.2.
OJ L 165, 30.4.2004 p. 1; corrected by OJ L 191, 28.5.2004, p. 1.
UL L 165, 30.4.2004, str. 1.
METHOD OF CALCULATING THE ENERGY VALUE OF POULTRYFEED
METODA IZRAČUNA ENERGIJSKE VREDNOSTI KRME ZA PERUTNINO
Method of calculation and expression of energy value
Metoda izračuna in prikaza energijske vrednosti
The energy value of compound poultry feed must be calculated in accordance with the formula set out below on the basis of the percentages of certain analytical components of the feed.
Energijska vrednost krme za perutnino mora biti izračunana v skladu s formulo, določeno v nadaljnjem besedilu na podlagi odstotkov nekaterih analitskih sestavin krme.
This value is to be expressed in megajoules (MJ) of metabolisable energy (ME), corrected for nitrogen, per kilogram of compound feed:
Ta vrednost se izrazi v megajoulih (MJ) presnovne energije (ME), popravljeno za dušik, na kilogram krmne mešanice:
MJ/kg of ME = 0,1551 × % crude protein +0,3431 × % crude fat +0,1669 × % starch +0,1301 × % total sugar (expressed as sucrose).
MJ/kg ME = 0,1551 × % surovih beljakovin +0,3431 × % surovih maščob +0,1669 × % škroba +0,1301 × % celotnega sladkorja (izraženega kot saharoza).
Tolerances applicable to declared values
Odstopanja, ki se uporabljajo za označene vrednosti
If the official inspection reveals a discrepancy (increased or reduced energy value of the feed) between the result of the inspection and the declared energy value, a minimum tolerance of 0,4 MJ/kg of ME shall be permitted.
Če se v okviru uradnega pregleda odkrije razlika (povečana ali zmanjšana energijska vrednost krme) med rezultatom pregleda in označeno energijsko vrednostjo, je dovoljeno minimalno odstopanje 0,4 MJ/kg ME.
After application of the above formula, the result obtained must be given to one decimal place.
Po uporabi zgoraj navedene formule je treba dobljeni rezultat izraziti z eno decimalko.
Sampling and analysis methods
Metode vzorčenja in analitske metode
Sampling of the compound feed and determination of the content of analytical components indicated in the method of calculation must be performed in accordance with the Community sampling methods and analysis methods for the official control of feed respectively.
Vzorčenje krmnih mešanic in določanje vsebnosti analitskih sestavin, navedenih v metodi izračuna, je treba izvesti v skladu z metodami Skupnosti za vzorčenje ali analitskimi metodami Skupnosti za uradni pregled krme.
The following are to be applied:
Uporablja se naslednje:
for determining the crude fat content: procedure B of the method for the determination of crude oils and fats, laid down in Part H of Annex III.
za določanje vsebnosti surove maščobe: postopek B metode za določanje surovih olj in maščob iz dela H Priloge III,
for determining the starch content: the polarimetric method, laid down in Part L of Annex III.
za določanje vsebnosti škroba: polarimetrična metoda iz dela L Priloge III.
METHODS OF ANALYSIS TO CONTROL ILLEGAL PRESENCE OF NO LONGER AUTHORISED ADDITIVES IN FEED
ANALITSKE METODE ZA NADZOR KRMNIH DODATKOV, KI NISO VEČ DOVOLJENI
Important notes:
Pomembno obvestilo:
More sensitive methods of analysis than the methods of analysis mentioned in this Annex can be used to detect the illegal presence of no longer authorised additives in feed.
Za določanje krmnih dodatkov, ki niso več dovoljeni, se lahko uporabljajo analitske metode, ki so občutljivejše od analitskih metod, navedenih v tej prilogi.
The methods of analysis mentioned in this Annex shall be used for confirmatory purposes.
Za potrditev se uporabljajo analitske metode iz te priloge.
DETERMINATION OF METHYL BENZOQUATE
DOLOČANJE METIL BENZOKVATA
7-benzyloxy-6-butyl-3-methoxycarbonyl-4-quinolone
7-benziloksi-6-butil-3-metoksikarbonil-4-kinolon
This method makes it possible to determine the level of methyl benzoquate in feed.
Ta metoda omogoča določanje ravni metil benzokvata v krmi.
Methyl benzoquate is extracted from the sample with methanolic methanesulfonic acid solution.
Metil benzokvat se ekstrahira iz vzorca z raztopino metanol metansulfonske kisline.
The extract is purified with dichloromethane, by ion-exchange chromatography and then again with dichloromethane.
Ekstrakt se prečisti z diklorometanom z ionsko izmenjevalno kromatografijo in nato ponovno z diklorometanom.
The methyl benzoquate content is determined by reversed-phase high-performance liquid chromatography (HPLC) with an UV detector.
Vsebnost metil benzokvata se določi s tekočinsko kromatografijo visoke ločljivosti (HPLC) z reverzno fazo in UV-detektorjem.
Mixture of methanol (3.2) and water (equivalent to HPLC grade) 75 + 25 (v + v).
mešanica metanola (3.2) in vode (za HPLC) 75 + 25 (v + v)
Filter through a 0,22 μm filter (4.5) and degas the solution (e.g. by ultrasonification for 10 minutes).
Filtrira se skozi filter 0,22 μm (4.5), raztopina pa se razplini (npr. 10 minut v ultrazvočni kopeli).
Methanesulfonic acid solution, c = 2 %
Raztopina metansulfonske kisline, c = 2 %
Dilute 20,0 ml methanesulfonic acid to 1000 ml with methanol (3.2).
20,0 ml metansulfonske kisline se razredči z metanolom (3.2) do 1000 ml.
Hydrochloric acid solution, c = 10 %
Raztopina klorovodikove kisline, c = 10 %
Dilute 100 ml hydrochloric acid (ρ201,18 g/ml) to 1000 ml with water.
100 ml klorovodikove kisline (ρ201,18 g/ml) se razredči z vodo do 1000 ml.