natgillin/translations-raw huggingface dataset:
Calibration solution of standard amino acids for use with hydrolysates, c = 5 nmol/50 μl of cysteic acid and methionine sulphone and c = 10 nmol/50 μl of the other amino acids.
Raztopina za umerjanje standardnih aminokislin, ki se uporablja s hidrolizati, c = 5 nmol/50 μl za cisteinsko kislino in metionin-sulfon ter c = 10 nmol/50 μl za druge aminokisline.
Transfer quantitatively to a 50 ml graduated flask and make up to the mark with citrate buffer (3.24) and mix.
Vsebina se prenese v 50-mililitrsko merilno bučko, dopolni se s citratnim pufrom (3.24) do oznake in premeša.
Store below 5 oC for not more than 3 months.
Hrani se največ 3 mesece pri temperaturi do 5 oC.
See also observation 9.1.
Glej tudi opombo 9.1.
Calibration solution of standard amino acids for use with hydrolysates prepared according to paragraph 5.3.3.1 and for use with extracts (5.2).
Raztopina za umerjanje standardnih aminokislin, ki se uporablja s hidrolizati, se pripravi v skladu s točko 5.3.3.1, za ekstrakte pa v skladu s točko 5.2.
The calibration solution is prepared according to 3.27.4 but omitting sodium chloride.
Raztopina za umerjanje se pripravi v skladu s točko 3.27.4, vendar brez natrijevega klorida.
100 or 250 ml round bottomed flask fitted with a reflux condenser.
100- ali 250-mililitrska bučka z okroglim dnom in kondenzatorjem povratnega toka
100 ml borosilicate glass bottle with screw cap with rubber/teflon liner (e.g. Duran, Schott) for use in the oven.
100-mililitrska borosilikatna steklena posoda z zamaškom z navojem in ovita v gumo/teflon (npr. Duran, Schott) za uporabo v sušilniku
Oven with forced ventilation and a temperature regulator with an accuracy better than ± 2 oC.
Sušilnik s prezračevanjem z umetnim dotokom zraka in regulatorjem temperature z natančnostjo, boljšo od ± 2 oC
pH-meter (three decimal places).
pH meter (na tri decimalke)
Membrane filter (0,22 μm).
Membranski filter (0,22 μm)
Centrifuge.
Centrifuga
Rotary vacuum evaporator.
Rotacijski vakuumski uparjalnik
Mechanical shaker or magnetic stirrer.
Mehanski stresalnik ali magnetno mešalo
Amino acid analyser or HPLC equipment with ion exchange column, device for ninhydrin, post column derivatisation and photometric detector.
Aminokislinski analizator ali oprema za HPLC s kolono z ionskim izmenjevalcem, pripravo za ninhidrin, derivatizacijo po koloni in fotometričnim detektorjem
The column is filled with sulfonated polystyrene resins capable of separating the amino acids from each other and from other ninhydrin-positive materials.
Kolona se napolni s smolo sulfoniranega polistirena, ki lahko loči aminokisline med seboj in od drugih materialov, ki vsebujejo ninhidrin.
The flow in the buffer and ninhydrin lines is provided by pumps having a flow stability of ±0,5 % in the period covering both the standard calibration run and the analysis of the sample.
Črpalki s stabilnim tokom ±0,5 % zagotavljata tok v pufrski in ninhidrinski črti pri standardnem umerjanju in analizi vzorca.
With some amino acid analysers hydrolysis procedures can be used in which the hydrolysate has a sodium concentration of c = 0,8 mol/l and contains all the residual formic acid from the oxidation step.
Za nekatere aminokisline se lahko uporabi hidroliza v analizatorju, pri čemer je koncentracija natrija v hidrolizatu c = 0,8 mol/l in v hidrolizatu je vsa mravljična kislina, ki nastane pri oksidaciji.
Others do not give a satisfactory separation of certain amino acids if the hydrolysate contains excess formic acid and/or high sodium ion concentrations.
Druge metode ne zagotavljajo zadovoljive ločitve nekaterih aminokislin, če je v hidrolizatu preveč mravljične kisline in/ali visoka koncentracija natrijevih ionov.
In this case the volume of acid is reduced by evaporation to approx. 5 ml after the hydrolysis and prior to pH adjustment.
V tem primeru se volumen kisline po hidrolizi in pred uravnavanjem pH zmanjša z uparevanjem na približno 5 ml.
The evaporation shall be performed under vacuum at 40 o C maximum.
Uparevanje se izvede pod vakuumom pri največ 40 oC.
The sample is ground to pass through a 0,5 mm sieve.
Vzorec se zdrobi, tako da preide skozi 0,5 mm sito.
Samples high in moisture must be either air-dried at a temperature not exceeding 50 oC or freeze dried prior to grinding.
Vzorce z visoko vsebnostjo vlage je treba pred drobljenjem osušiti na zraku pri temperaturi do 50 oC ali pa osušiti z zmrzovanjem.
Samples with a high fat content shall be extracted with light petroleum (3.12) prior to grinding.
Vzorci z visoko vsebnostjo maščobe se pred drobljenjem ekstrahirajo s petroletrom (3.12).
Determination of free amino acids in feed and premixtures
Določanje prostih aminokislin v krmi in premiksih
Weigh to the nearest 0,2 mg an appropriate amount (1-5 g) of the prepared sample (5.1), into a conical flask and add 100,0 ml of extraction mixture (3.21).
Primerna količina (1–5 g) pripravljenega vzorca (5.1) se natehta na 0,2 mg natančno in prenese v erlenmajerico, temu pa se doda 100,0 ml mešanice ekstrakcije (3.21).
Shake the mixture for 60 min. using a mechanical shaker or a magnetic stirrer (4.8).
Z mehanskim stresalnikom ali magnetnim mešalom (4.8) se mešanica stresa 60 minut.
Allow the sediment to settle and pipette 10,0 ml of the supernatant solution into a 100 ml beaker.
Pusti se, da se usedlina usede na dno, in odpipetira 10,0 ml supernatanta v 100-mililitrsko čašo.
Add 5,0 ml of sulfosalicylic acid solution (3.22), with stirring and continue to stir with the aid of magnetic stirrer for 5 min. Filter or centrifuge the supernatant in order to remove any precipitate.
Med mešanjem se doda 5,0 ml raztopine sulfosalicilne kisline (3.22) in 5 minut nadaljuje z mešanjem s pomočjo magnetnega mešala. Supernatant se filtrira ali centrifugira, da se odstrani ves precipitat.
Place 10,0 ml of the resulting solution into a 100 ml beaker and adjust the pH to 2,20 using sodium hydroxide solution (3.18), transfer to a volumetric flask of appropriate volume using citrate buffer (3.24), and make up to the mark with the buffer solution (3.24).
10,0 ml te raztopine se prenese v 100-mililitrsko čašo in pH uravnava na 2,20 z raztopino natrijevega hidroksida (3.18), s citratnim pufrom (3.24) se prenese v merilno bučko ustreznega volumna in dopolni z raztopino pufra (3.24) do oznake.
If an internal standard is being used add 1,00 ml of internal standard (3.27.3) for each 100 ml final solution and make up to the mark with the buffer solution (3.24).
Če se uporablja notranji standard, se za vsakih 100 ml končne raztopine doda 1,00 ml notranjega standarda (3.27.3) ter dopolni z raztopino pufra (3.24) do oznake.
Proceed to the chromatography step according to paragraph 5.4.
Nadaljuje se s kromatografijo v skladu s točko 5.4.
If the extracts are not being examined the same day, they must be stored below 5 oC.
Če se ekstrakti ne pregledajo isti dan, se morajo hraniti pri temperaturi do 5 oC.
Determination of total amino acids
Določanje celotnih aminokislin
Weigh to the nearest 0,2 mg from 0,1 to 1 g of the prepared sample (5.1) into:
0,1–1 g pripravljenega vzorca (5.1) se natehta na 0,2 mg natančno in prenese v:
a 100 ml round-bottomed flask (4.1) for open hydrolysis (5.3.2.3) or,
100-mililitrsko bučko z okroglim dnom (4.1) (za odprto hidrolizo 5.3.2.3) ali
a 250 ml round-bottomed flask (4.1) if a low sodium concentration is required (5.3.3.1) or,
250-mililitrsko bučko z okroglim dnom (4.1), če je zahtevana nizka koncentracija natrija (5.3.3.1), ali
a 100 ml bottle fitted with a screw cap (4.2), for closed hydrolysis (5.3.2.4).
100-mililitrsko steklenico z zamaškom z navojem (4.2) (za zaprto hidrolizo 5.3.2.4).
The weighed sample portion must have a nitrogen content of about 10 mg and a moisture content not exceeding 100 mg.
V natehtanem vzorčenem deležu mora biti vsebnost dušika približno 10 mg, vsebnost vlage pa ne sme preseči 100 mg.
Place the flask/bottle in an ice-water bath and cool to 0 oC, add 5 ml of oxidation mixture (3.23) and mix using a glass spatula with a bent tip.
Bučka/steklenica se postavi v ledeno kopel in ohladi na 0 oC, doda se 5 ml oksidacijske mešanice (3.23) in meša s stekleno lopatico z ukrivljeno konico.
Proceed to 5.3.2.1.
Nadaljuje se s točko 5.3.2.1.
Hydrolysis of oxidised samples
Hidroliza oksidiranih vzorcev
To the oxidised sample prepared according to 5.3.1 add 25 ml of hydrolysis mixture (3.20) taking care to wash down any sample residue adhering to the sides of the vessel and the spatula.
Oksidiranemu vzorcu, pripravljenemu v skladu s točko 5.3.1, se doda 25 ml hidrolizne mešanice (3.20), pri čemer je treba pazljivo sprati kakršne koli ostanke vzorca s stene posode in lopatice.
Depending on the hydrolysis procedure being used, proceed according to 5.3.2.3 or 5.3.2.4.
Nadaljuje se v skladu s točko 5.3.2.3 ali 5.3.2.4 odvisno od uporabljenega postopka hidrolize.
Hydrolysis of unoxidised samples
Hidroliza neoksidiranih vzorcev
Weigh into either a 100 ml or a 250 ml round-bottom flask (4.1) or a 100 ml bottle fitted with a screw cap (4.2), to the nearest 0,2 mg, from 0,1 to 1 g of the prepared sample (5.1).
V 100- ali 250-mililitrsko bučko z okroglim dnom (4.1) ali 100-mililitrsko steklenico z zamaškom z navojem (4.2) se natehta 0,1–1 g pripravljenega vzorca (5.1) na 0,2 mg natančno.
The weighed sample portion must have a nitrogen content of about 10 mg.
V natehtanem vzorčenem deležu mora biti vsebnost dušika približno 10 mg.
Add carefully 25 ml of hydrolysis mixture (3.20) and mix with the sample.
Pazljivo se doda 25 ml hidrolizne mešanice (3.20) in zmeša z vzorcem.
Proceed according to either 5.3.2.3 or 5.3.2.4.
Nadaljuje se v skladu s točko 5.3.2.3 ali 5.3.2.4.
Open hydrolysis
Odprta hidroliza
Disconnect the flask and cool it in an ice bath.
Bučka se odstrani in ohladi v ledeni kopeli.
Proceed according to 5.3.3.
Nadaljuje se v skladu s točko 5.3.3.
Closed Hydrolysis
Zaprta hidroliza
Place the bottle containing the mixture prepared in accordance with 5.3.2.1 or 5.3.2.2 in an oven (4.3) at 110 oC.
Steklenica, ki vsebuje mešanico, pripravljeno v skladu s točko 5.3.2.1 ali 5.3.2.2, se postavi v sušilnik (4.3) pri 110 oC.
During the first hour in order to prevent a build up of pressure (due to the evolution of gaseous substances) and to avoid explosion, place the screw cap over the top of the vessel.
V prvi uri se za preprečitev povečanja tlaka (zaradi nastanka plinastih snovi) in eksplozije zamašek z navojem postavi čez vrh steklenice.
Do not close the vessel with the cap.
Steklenica se ne sme zapreti z zamaškom.
Leave to cool.
Pusti se, da se ohladi.
Depending on the procedure for pH adjustment (5.3.3), quantitatively transfer the contents of the bottle to a 250 ml beaker or a 250 ml round-bottom flask, using citrate buffer (3.24).
Odvisno od postopka za uravnavanje pH (5.3.3) se vsebina steklenice z uporabo citratnega pufra (3.24) prenese v 250-mililitrsko čašo ali 250-mililitrsko bučko z okroglim dnom.
Adjustment of pH
Uravnavanje pH
Depending on the sodium tolerance of the amino acid analyser (4.9) proceed according to 5.3.3.1 or 5.3.3.2 for the pH adjustment.
Glede na odstopanje analizatorja aminokislin (4.9) za natrij se za uravnavanje pH nadaljuje v skladu točko 5.3.3.1 ali 5.3.3.2.
For Chromatographic Systems (4.9) requiring a low sodium concentration
Kromatografski sistemi (4.9), pri katerih je zahtevana nizka koncentracija natrija
It is advisable to use an internal stock standard solution (3.27.3) when amino acid analysers requiring a low sodium concentration are employed (when the acid volume has to be reduced).
Ko se uporabljajo analizatorji aminokislin, pri katerih je zahtevana nizka koncentracija natrija (ko je treba zmanjšati volumen kisline), je priporočljivo uporabiti raztopino notranjega osnovnega standarda (3.27.3).
In this case add 2,00 ml of the internal stock standard solution (3.27.3) to the hydrolysate before the evaporation.
V tem primeru se hidrolizatu pred uparevanjem dodata 2,00 ml raztopine notranjega osnovnega standarda (3.27.3).
Add 2 drops of 1-octanol (3.15) to the hydrolysate obtained in accordance with paragraph 5.3.2.3 or 5.3.2.4.
Hidrolizatu, pridobljenemu v skladu s točko 5.3.2.3 ali 5.3.2.4, se dodata 2 kaplji 1-oktanola (3.15).
Using a rotary evaporator (4.7) reduce the volume to 5-10 ml under vacuum at 40 oC.
Z rotacijskim uparilnikom (4.7) se pod vakuumom pri 40 oC volumen zmanjša na 5–10 ml.
If the volume is accidentally reduced to less than 5 ml the hydrolysate must be discarded and the analysis recommenced.
Če se volumen nenamerno zmanjša na manj kot 5 ml, se mora hidrolizat izločiti in analiza začeti znova.
Adjust the pH to 2,20 with sodium hydroxide solution (3.18) and proceed to paragraph 5.3.4.
pH se uravna na 2,20 z raztopino natrijevega hidroksida (3.18), nato pa se nadaljuje v skladu s točko 5.3.4.
For all other Amino Acid Analysers (4.9)
Za vse druge analizatorje aminokislin (4.9)
Take the hydrolysates obtained in accordance with 5.3.2.3 or 5.3.2.4 and partly neutralise them by carefully adding with stirring, 17 ml of sodium hydroxide solution (3.17), ensuring that the temperature is kept below 40 oC.
Hidrolizati, pridobljeni v skladu s točko 5.3.2.3 ali 5.3.2.4, se delno nevtralizirajo, tako da se med mešanjem pazljivo doda 17 ml raztopine natrijevega hidroksida (3.17), pri čemer se mora ohranjati temperatura pod 40 oC.
Adjust the pH to 2,20 at room temperature using sodium hydroxide solution (3.17) and finally sodium hydroxide solution (3.18).
Pri sobni temperaturi se pH uravna na 2,20 z raztopino natrijevega hidroksida (3.17) in nato raztopino natrijevega hidroksida (3.18).
Proceed to 5.3.4.
Nadaljuje se v skladu s točko 5.3.4.
Sample solution for chromatography
Raztopina vzorca za kromatografijo
Quantitatively transfer the pH adjusted hydrolysate (5.3.3.1 or 5.3.3.2) with citrate buffer (3.24) to a 200 ml graduated flask, and make up to the mark with buffer (3.24).
Hidrolizat (5.3.3.1 ali 5.3.3.2), uravnan na pH, se s citratnim pufrom (3.24) prenese v 200-mililitrsko merilno bučko in dopolni s pufrom (3.24) do oznake.
If an internal standard has not already been used, add 2,00 ml of internal standard (3.27.3) and make up to the mark with citrate buffer (3.24).
Če notranji standard še ni bil uporabljen, se dodata 2,00 ml notranjega standarda (3.27.3), nato pa se dopolni s citratnim pufrom (3.24) do oznake.
Mix thoroughly.
Temeljito se premeša.
Proceed to the chromatography step (5.4).
Nadaljuje se s kromatografijo (5.4).
If the sample solutions are not being examined the same day they must be stored below 5 oC.
Če se raztopine vzorca ne pregledajo še isti dan, se morajo shraniti pri temperaturi pod 5 oC.
Before chromatography bring the extract (5.2) or hydrolysate (5.3.4) to room temperature.
Pred kromatografijo mora biti temperatura ekstrakta (5.2) ali hidrolizata (5.3.4) enaka sobni temperaturi.
Shake the mixture and filter a suitable amount through a 0,22 μm membrane filter (4.5).
Mešanica se stresa, primerna količina pa se filtrira skozi membranski filter 0,22 μm (4.5).
The resulting clear solution is subjected to ion exchange chromatography, using an amino acid analyser (4.9).
S to bistro raztopino se opravi ionska izmenjevalna kromatografija z analizatorjem aminokislin (4.9).
The injection may be performed manually or automatically.
Vbrizgavanje je lahko ročno ali avtomatično.
It is important that the same quantity of solution ±0,5 % is added to the column for the analysis of standards and samples except when an internal standard is used, and that the sodium:amino acid ratios in the standard and sample solutions are as similar as is practicable.
Pomembno je, da se ista količina raztopine (±0,5 %) doda koloni za analizo standardov in vzorcev, razen ko se uporablja notranji standard, ter da je razmerje med natrijem in aminokislino v raztopinah standarda in vzorca takšno, kot se uporablja ponavadi.
In general the frequency of calibration runs depends on the stability of the ninhydrin reagent and the analytical system.
Na splošno je pogostost umerjanja odvisna od stabilnosti reagenta ninhidrina in analitskega sistema.
The standard or sample is diluted with citrate buffer (3.24) to give a peak area of the standard of 30 %-200 % of the sample amino acid peak area.
Standard ali vzorec se razredči s citratnim pufrom (3.24), da se doseže površina vrha standarda, ki je 30–200 % površine vrha aminokislin v vzorcu.
The chromatography of amino acids will vary slightly according to the type of analyser employed and resin used.
Kromatografija aminokislin se nekoliko razlikuje glede na vrsto uporabljenega analizatorja in smole.
The chosen system must be capable of separating the amino acids from each other and from the ninhydrin-positive materials.
Izbrani sistem mora imeti možnost ločitve aminokisline med seboj in od materialov, ki vsebujejo ninhidrin.
In the range of operation the chromatographic system must give a linear response to changes in the amounts of amino acids added to the column.
V delovnem območju se mora kromatografski sistem odzivati linearno na spremembe količin aminokislin, dodanih koloni.
During the chromatography step the valley:peak height ratios mentioned below apply, when an equimolar solution (of the amino acids being determined) is analysed.
Pri kromatografiji se uporabljajo spodaj navedena razmerja med najnižjimi in najvišjimi vrednostmi, ko se analizira istomolarna raztopina (aminokislin, ki se določajo).
This equimolar solution must contain at least 30 % of the maximum load of each amino acid which can be accurately measured with the amino acid analyser system (4.9).
Istomolarna raztopina mora vsebovati vsaj 30 % največje vsebnosti vsake aminokisline, ki se lahko natančno izmeri s sistemom za analizo aminokislin (4.9).
For separation of threonine-serine the valley:peak height ratio of the lower of the two overlapping amino acids on the chromatogram must not exceed 2:10.
Za ločevanje treonina od serina razmerje med najnižjo in najvišjo vrednostjo za aminokislino z nižjo vrednostjo izmed dveh prekrivajočih se aminokislin ne sme presegati 2:10.
(if only cyst(e)ine, methionine, threonine and lysine are determined, insufficient separation from adjoining peaks will adversely influence the determination).
(Če se določajo le cistein, metionin, treonin in lizin, bo neustrezna ločitev od sosednjih vrhov negativno vplivala na določanje.)
For all other amino acids the separation must be better than 1:10.
Za vse druge aminokisline mora biti ločevanje boljše od 1:10.
The system must ensure that lysine is separated from ‘lysine artifacts’ and ornithine.
Sistem mora zagotavljati, da se lizin loči od „lizinskih artefaktov“ in ornitina.
The area of the sample and standard peaks is measured for each individual amino acid and the amount (X), in g amino acid per kg sample, is calculated as follows:
Za vsako posamezno aminokislino se izračuna površina vzorca in najvišje vrednosti standarda ter količina (X), izražena v g aminokislin na kg vzorca.
If an internal standard is used multiply by: D
Če se uporablja notranji standard, se pomnoži z: D
A peak area, hydrolysate or extract
A površina vrha hidrolizata ali ekstrakta;
B peak area, calibration standard solution
B površina vrha standardne raztopine za umerjanje;
C peak area, internal standard in hydrolysate or extract
C površina vrha hidrolizata ali ekstrakta kot notranjega standarda;
D peak area, internal standard, calibration standard solution
D površina vrha standardne raztopine za umerjanje kot notranjega standarda;
M molar weight of the amino acid being determined
M molska masa aminokisline, ki se določa;
c concentration of standard in μmol/ml
c koncentracija standarda v μmol/ml;
m sample weight (g) (corrected to original weight if dried or defatted)
m masa vzorca v gramih (popravljena na prvotno maso, če je vzorec posušen ali razmaščen);
V ml total hydrolysate (5.3.4) or ml calculated total dilution volume of extract (6.1)
V ml celotnega hidrolizata (5.3.4) ali izračunani celotni volumen razredčitve ekstrakta (6.1) v ml.
Cystine and cysteine are both determined as cysteic acid in hydrolysates of oxidised sample, but calculated as cystine (C6H12N2O4S2, M 240,30 g/mol) by using M 120,15 g/mol (= 0,5 x 240,30 g/mol).
Cistin in cistein se določata kot cisteinska kislina v hidrolizatih oksidiranega vzorca, vendar se izračunata kot cistin (C6H12N2O4S2, M 240,30 g/mol) z uporabo molske mase 120,15 g/mol (= 0,5 x 240,30 g/mol).
Methionine is determined as methionine sulphone in hydrolysates of oxidised sample, but calculated as methionine by using M of methionine: 149,21 g/mol.
Metionin se določa kot metionin-sulfon v hidrolizatih oksidiranega vzorca, vendar se izračuna kot metionin, pri čemer se uporabi molska masa metionina: 149,21 g/mol.
Added free methionine is determined after extraction as methionine, for the calculation the same M is used.
Dodani prosti metionin se določa po ekstrakciji kot metionin, za izračun pa se uporabi ista molska masa.
The total dilution volume of extracts (F) for determination of free amino acids (5.2) is calculated as follows:
Celotni volumen razredčitve ekstraktov (F) za določanje prostih aminokislin (5.2) se izračuna po naslednji formuli:
V Volume of final extract
V volumen končnega ekstrakta.
Evaluation of the method
Ocena metode
The method has been tested in an intercomparison made at international level in 1990 using four different feed (mixed pig feed, broiler compound, protein concentrate, premixture).
Metoda je bila preskušena v mednarodni medlaboratorijski primerjavi, ki je bila izvedena leta 1990 s štirimi različnimi vrstami krme (mešana prašičja krma, mešanica za pitovne piščance, beljakovinski koncentrat, premiks).
The results, after elimination of outliers, of mean and standard deviation are given in the tables in this point:
Rezultati, brez izjem, s srednjimi vrednostmi in standardnimi odmiki so prikazani v preglednici v tej točki:
Means in g/kg
Srednje vrednosti v g/kg
n Number of participating laboratories.
n število sodelujočih laboratorijev.
Reference material
Referečni material
Amino Acid
Aminokislina
Cyst(e)ine
Cist(e)in
Mixed Pig Feed
Mešana prašičja krma
Broiler Compound
Mešanica za pitovne piščance
Protein Concentrate
Beljakovinski koncentrat
The repeatability expressed as ‘within laboratory standard deviation’ of the abovementioned intercomparison is given in the tables below:
Ponovljivost zgoraj navedene primerjave, izražena kot „standardni odmik v laboratoriju“, je prikazana v spodnji preglednici:
Within Laboratory Standard Deviation (Sr) in g/kg
Standardni odmik (Sr) v laboratoriju, izražen v g/kg
Coefficient of Variation (%) for Within Laboratory Standard Deviation (Sr)
Koeficient variacije (%) standardnega odmika (Sr) v laboratoriju
The results for between laboratory standard deviation by the abovementioned intercomparison are given in the table below:
Rezultati zgoraj navedene primerjave, izraženi kot standardni odmik med laboratoriji, so prikazani v spodnji preglednici:
Between Laboratory Standard Deviation (SR) in g/kg
Standardni odmik (SR) med laboratoriji, izražen v g/kg
Coefficient of Variation (%) for Between Laboratory Standard Deviation (SR)
Koeficient variacije (%) standardnega odmika (SR) med laboratoriji
Use of reference materials
Uporaba referenčnih materialov
The correct application of the method shall be verified by making replicate measurements of certified reference materials when available.
Pravilna uporaba metode se preveri s ponovljenimi merjenji na potrjenih referenčnih materialih, kadar so na voljo.
Calibration with certified amino acid calibration solution is recommended.
Priporočeno je umerjanje s potrjeno raztopino za umerjanje aminokislin.
Because of differences between amino acid analysers the final concentrations of the calibration solutions of standard amino acids (see 3.27.4 and 3.27.5) and of the hydrolysate (see 5.3.4) shall be taken as a guideline.
Zaradi razlik med analizatorji aminokislin se kot smernica obravnavajo končne koncentracije raztopin standardnih aminokislin za umerjanje (glej 3.27.4 in 3.27.5) in hidrolizata (glej 5.3.4).
The range of linear response of the apparatus has to be checked for all amino acids.
Za vse aminokisline je treba preveriti območje naprave, v katerem je odziv linearen.