natgillin/translations-raw huggingface dataset:
To fortify at a level of 1 mg/kg add 0,1 ml of the stock standard solution (3.8.1) to 50 g of a blank feed, mix thoroughly and leave for 10 min. mixing again several times before proceeding (5.2).
Za koncentracijo l mg/kg se doda 0,1 ml osnovne standardne raztopine (3.8.1) v 50 g slepe krme, dobro se premeša in pusti 10 minut, pred nadaljevanjem (5.2) pa se večkrat ponovno premeša.
Alternatively, if a blank feed similar in type to that of the sample is not available (see 5.1.1), a recovery test can be performed by means of the standard addition method.
Če pa vrsta slepe krme, podobna vrsti vzorca, ni na voljo (glej točko 5.1.1), se lahko preskus izkoristka izvede po metodi standardnega dodatka.
In this case, the sample to be analysed is fortified with a quantity of diclazuril, similar to that already present in the sample.
V tem primeru se vzorcu za analizo doda količina diklazurila, podobna tisti v vzorcu.
This sample is analysed, together with the unfortified sample and the recovery can be calculated by subtraction.
Ta vzorec se analizira skupaj z vzorcem brez dodanega karbadoksa, izkoristek pa se izračuna z odštevanjem.
Weigh to the nearest 0,01 g approximately 50 g of the sample.
Približno 50 g vzorca se natehta na 0,01 g natančno.
Transfer to a 500 ml conical flask, add 1,00 ml internal standard solution (3.9.2), 200 ml extraction solvent (3.12) and stopper the flask.
Prenese se v 500-mililitrsko erlenmajerico, doda se 1,00 ml raztopine notranjega standarda (3.9.2) in 200 ml ekstrakcijskega topila (3.12), nato pa se erlenmajerica zamaši.
Shake the mixture on the shaker (4.1) overnight.
Mešanica se prek noči stresa v stresalniku (4.1).
Allow to settle for 10 minutes.
Pusti se 10 minut, da se usede.
Transfer a 20 ml aliquot of the supernatant to a suitable glass container and dilute with 20 ml water.
20-mililitrski alikvot supernatanta se prenese v primerno stekleno posodo in razredči z 20 ml vode.
Transfer this solution on an extraction cartridge (3.11), and pass through by applying vacuum (4.5).
Ta raztopina se prenese na ekstrakcijsko kartušo (3.11), skoznjo pa se spusti z vakuumom (4.5).
Wash the cartridge with 25 ml of a mixture of extraction solvent (3.12) and water, 65 + 35 (V + V).
Kartuša se spere s 25 ml mešanice ekstrakcijskega topila (3.12) in vode, 65 + 35 (V + V).
Discard the collected fractions and elute the compounds with 25 ml of a mixture of extraction solvent (3.12) and water, 80 + 20 (V + V).
Zbrane frakcije se zavržejo, spojine pa se eluirajo s 25 ml mešanice ekstrakcijskega topila (3.12) in vode, 80 + 20 (V + V).
Evaporate this fraction until it had just reached dryness by means of the rotary evaporator (4.3) at 60 oC.
Ta frakcija se upari v rotacijskem uparjalniku (4.3) pri 60 oC do prve sušine.
Dissolve the residue in 1,0 ml DMF (3.6), add 1,5 ml of water (3.1) and mix.
Ostanek se raztopi v 1,0 ml DMF (3.6), doda se 1,5 ml vode (3.1) in premeša.
Filter through a membrane filter (4.4).
Filtrira se skozi membranski filter (4.4).
Proceed to the HPLC determination (5.3).
Nadaljuje se z določanjem s HPLC (5.3).
Weigh to the nearest 0,001 g approximately 1 g of the sample.
Približno 1 g vzorca se natehta na 0,001 g natančno.
Transfer to a 500 ml conical flask, add 1,00 ml internal standard solution (3.9.3), 200 ml extraction solvent (3.12) and stopper the flask.
Prenese se v 500-mililitrsko erlenmajerico, doda se 1,00 ml raztopine notranjega standarda (3.9.3) in 200 ml ekstrakcijskega topila (3.12), nato pa se erlenmajerica zamaši.
Shake the mixture overnight on the shaker (4.1).
Mešanica se prek noči stresa v stresalniku (4.1).
Transfer an aliquot of 10000/p ml (p = nominal content of diclazuril in the premix in mg/kg) of the supernatant to a round bottomed flask of suitable size.
Alikvot (10000/p ml, p = nominalna vsebnost diklazurila v mg/kg v premiksu) supernatanta se prenese v primerno veliko bučko z okroglim dnom.
Evaporate until it had just reached dryness, under reduced pressure at 60 oC by means of the rotary evaporator (4.3).
Do prve sušine se upari pri znižanem tlaku in 60 oC v rotacijskem uparjalniku (4.3).
Redissolve the residue in 10,0ml DMF (3.6), add 15,0 ml water (3.1) and mix.
Ostanek se ponovno raztopi v 10,0 ml DMF (3.6), doda se 15,0 ml vode (3.1) in premeša.
The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results.
Naslednji pogoji se lahko uporabljajo kot smernice, lahko pa se uporabljajo drugi pogoji, če zagotavljajo enakovredne rezultate.
Liquid chromatographic column (4.2.1)
Kolona za tekočinsko kromatografijo (4.2.1):
100 mm × 4,6 mm, Hypersil ODS, 3 μm packing, or equivalent
100 mm × 4,6 mm, Hypersil ODS, polnitev 3 μm ali enakovredna
Aqueous solution of ammonium acetate and tetrabutyl-ammonium hydrogen sulphate
vodna raztopina amonijevega acetata in tetrabutil amonijevega hidrogen sulfata
Elution mode:
Način eluiranja:
linear gradient
linearni gradient
initial conditions: A + B + C = 60 + 20 + 20 (V + V + V)
začetni pogoji: A + B + C = 60 + 20 + 20 (V + V + V)
after 10 min. gradient elution during 30 min. to: A + B + C = 45 + 20 + 35 (V + V + V)
po 10 minutah se elucijski gradient v 30 minutah spremeni na: A + B + C = 45 + 20 + 35 (V + V + V).
Flush with B during 10 min.
V 10 minutah se spere z B.
Injection volume:
Volumen, ki se vbrizga:
Detector wavelength:
Valovna dolžina detekcije:
Check the stability of the chromatographic system, injecting several times the calibration solution (3.10), containing 2 μg/ml, until constant peak heights and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizgavanjem raztopine za umerjanje s koncentracijo 2 μg/ml (3.10), dokler se ne dosežejo konstantne višine vrhov in konstantni retencijski časi.
Calibration solution
Raztopina za umerjanje
Inject 20 μl of the calibration solution (3.10) several times and determine the mean peak height (area) of the diclazuril and internal standard peaks.
Večkrat se vbrizga 20 μl raztopine za umerjanje (3.10) ter določi srednja vrednost višine (površine) vrhov diklazurila in vrhov raztopine notranjega standarda.
Inject 20 μl of the sample solution (5.2.1 or 5.2.2) several times and determine the mean peak height (area) of the diclazuril and internal standard peaks.
Večkrat se vbrizga 20 μl raztopine vzorca (5.2.1 ali 5.2.2) ter določi srednja vrednost višine (površine) vrha diklazurila in raztopine notranjega standarda.
Feeds
Krma
The diclazuril content w (mg/kg) in the sample is given by the following formula:
Vsebnost diklazurila (w) (mg/kg) v vzorcu se izračuna po naslednji formuli:
peak height (area) of diclazuril in the sample solution (5.2.1)
višina (površina) vrha diklazurila v raztopini vzorca (5.2.1);
peak height (area) of the internal standard in the sample solution (5.2.1)
višina (površina) vrha notranjega standarda v raztopini vzorca (5.2.1);
peak height (area) of diclazuril in the calibration solution (3.10)
višina (površina) vrha diklazurila v raztopini za umerjanje (3.10);
peak height (area) of the internal standard in the calibration solution (3.10)
višina (površina) vrha notranjega standarda v raztopini za umerjanje (3.10);
diclazuril concentration in the calibration solution in μg/ml (3.10)
koncentracija diklazurila v raztopini za umerjanje v μg/ml (3.10);
V volume of the sample extract according to 5.2.1 (i.e. 2,5 ml)
V volumen ekstrakta vzorca v skladu s točko 5.2.1 (tj. 2,5 ml).
peak height (area) of diclazuril in the sample solution (5.2.2)
višina (površina) vrha diklazurila v raztopini vzorca (5.2.2);
peak height (area) of the internal standard in the sample solution (5.2.2)
višina (površina) vrha notranjega standarda v raztopini vzorca (5.2.2);
V volume of the sample extract according to 5.2.2 (i.e. 25 ml)
V volumen ekstrakta vzorca v skladu s točko 5.2.2 (tj. 25 ml);
p nominal content of diclazuril in mg/kg in the premixture
p nominalna vsebnost diklazurila v mg/kg v premiksu.
The identity of the analyte can be confirmed by co-chromatography, or by using a diode-array detector by which the spectra of the sample extract (5.2.1 or 5.2.2) and the calibration solution (3.10) are compared.
Identiteta analita se lahko potrdi z dodatno kromatografijo ali z detektorjem s serijo diod, s katerim se primerjata spekter ekstrakta vzorca (5.2.1 ali 5.2.2) in spekter raztopine za umerjanje (3.10).
A sample extract (5.2.1 or 5.2.2) is fortified by addition of an appropriate amount of calibration solution (3.10).
Ekstraktu vzorca (5.2.1 ali 5.2.2) se doda primerna količina raztopine za umerjanje (3.10).
The amount of added diclazuril must be similar to the amount of diclazuril found in the sample extract.
Količina dodanega diklazurila mora biti podobna količini diklazurila v ekstraktu vzorca.
Only the height of the diclazuril peak and the internal standard peak shall be enhanced after taking into account both the amount added and the dilution of the extract.
Povišana sta le vrh diklazurila in vrh notranjega standarda ob upoštevanju dodane količine in razredčitve ekstrakta.
The peak width, at half of its height, must be within ± 10 % of the original width of the diclazuril peak or the internal standard peak of the unfortified sample extract.
Širina vrha na polovici višine mora biti znotraj ± 10 % prvotne širine vrha diklazurila ali notranjega standardnega vrha v vzorcu, ki mu diklazuril ni bil dodan.
The wavelength of maximum absorption of the sample and of the standard spectra, recorded at the peak apex on the chromatogram, must be the same within a margin determined by the resolving power of the detection system.
valovni dolžini maksimalne absorpcije spektra vzorca in spektra standarda, zabeleženi na najvišji točki vrha na kromatogramu, morata biti znotraj meja, določenih z ločljivostjo sistema za detekcijo.
For diode-array detection this is typically within ± 2 nm.
Pri detekciji s serijo diod je to ponavadi ± 2 nm;
Between 230 and 320 nm, the sample and standard spectra recorded at the peak apex of the chromatogram, must not be different for those parts of the spectrum within the range 10 % 100 % of relative absorbance.
med 230 in 320 nm se spekter vzorca in spekter standarda, zabeležena na najvišji točki vrha na kromatogramu, za navedene dele spektra ne smeta razlikovati v območju 10–100 % relativne absorbance.
This criterion is met when the same maxima are present and at no observed point the deviation between the two spectra exceeds 15 % of the absorbance of the standard analyte.
To merilo je izpolnjeno, ko so prisotne iste najvišje vrednosti in ko odmik med dvema spektroma na nobeni opazovani točki ne preseže 15 % absorbance standardnega analita;
Between 230 and 320 nm, the spectra of the upslope, apex and downslope of the peak produced by the sample extract must not be different from each other for those parts of the spectrum within the range 10 % 100 % of relative absorbance.
med 230 in 320 nm se spektri naraščanja, najvišje točke vrha in padanja vrha, pridobljeni iz ekstrakta vzorca, za navedene dele spektra ne smejo razlikovati v območju 10–100 % relativne absorbance.
This criterion is met when the same maxima are present and when at all observed points the deviation between the spectra does not exceed 15 % of the absorbance of the spectrum of the peak apex.
To merilo je izpolnjeno, ko so prisotne iste najvišje točke in ko odmik med spektri na nobeni od opazovanih točk ne preseže 15 % absorbance spektra najvišje točke.
30 % relative, to the higher value for diclazuril contents from 0,5 mg/kg to 2,5 mg/kg,
30 % višje vrednosti za vsebnosti diklazurila 0,5–2,5 mg/kg,
0,75 mg/kg for diclazuril contents between 2,5 mg/kg and 5 mg/kg,
0,75 mg/kg za vsebnosti diklazurila 2,5–5 mg/kg,
15 % relative to the higher value for diclazuril contents of more than 5 mg/kg.
15 % višje vrednosti za vsebnosti diklazurila nad 5 mg/kg.
For a fortified (blank) sample the recovery shall be at least 80 %.
Pri vzorcu z dodatkom (slepem vzorcu) je izkoristek najmanj 80 %.
A collaborative study was arranged in which 5 samples were analysed by 11 laboratories.
Organizirana je bila medlaboratorijska študija, pri kateri je 11 laboratorijev analiziralo 5 vzorcev.
These samples consisted of two premixtures; one was mixed with an organic matrix (O 100) and the other with an inorganic matrix (A 100).
Te vzorce sta sestavljala dva premiksa; eden je bil mešan z organsko matrico (O 100), drug pa z anorgansko matrico (A 100).
The theoretical content is 100 mg diclazuril per kg.
Teoretična vsebnost je 100 mg diklazurila na kg.
The three mixed feeds for poultry were made by 3 different producers (NL) (L1/Z1/K1).
Trije različni proizvajalci (NL) (L1/Z1/K1) so proizvedli 3 mešane perutninske krme.
The theoretical content is 1 mg diclazuril per kg.
Teoretična vsebnost je 1 mg diklazurila na kg.
The laboratories were instructed to analyse each of the samples once or in duplicate.
Laboratorijem je bilo naročeno, naj vsak vzorec analizirajo enkrat ali dvakrat.
(More detailed information on this collaborative study can be found in the Journal of AOAC International, Volume 77, No 6, 1994, p. 1359-1361).
(Podrobnejše informacije o navedeni medlaboratorijski študiji so v Journal of AOAC International, knjiga 77, št. 6, 1994, str. 1359–1361).
The results are given in the following table.
Rezultati so navedeni v spodnji preglednici.
Nominal content (mg/kg)
Nominalna vsebnost (mg/kg)
The diclazuril response must have been previously demonstrated to be linear over the range of concentrations being measured.
Predhodno je treba dokazati, da je odziv diklazurila linearen v območju koncentracij, ki se merijo.
DETERMINATION OF LASALOCID SODIUM
DOLOČANJE LASALOCID NATRIJA
Sodium salt of a polyether monocarboxylic acid produced by Streptomyces lasaliensis
Natrijeva sol polieter monokarboksilne kisline, proizvedena s Streptomyces lasaliensis
The method makes it possible to determine the level of lasalocid sodium in feed and premixtures.
Ta metoda omogoča določanje ravni lasalocid natrija v krmi in premiksih.
The limit of detection is 5 mg/kg, the limit of quantification is 10 mg/kg.
Meja določanja je 5 mg/kg, meja kvantifikacije pa 10 mg/kg.
Lasalocid sodium is extracted from the sample into acidified methanol and determined by reversed-phase high performance liquid chromatography (HPLC) using a spectrofluorometric detector.
Lasalocid natrij se ekstrahira iz vzorca v nakisani metanol in določi s tekočinsko kromatografijo visoke ločljivosti (HPLC) z reverzno fazo z uporabo spektrofluorimetričnega detektorja.
Potassium dihydrogen phosphate (KH2PO4).
Kalijev dihidrogen fosfat (KH2PO4)
Orthophosphoric acid, w (w/w) = 85 %.
Ortofosforna kislina, m (m/m) = 85 %
Orthophosphoric acid solution, c = 20 %.
Raztopina ortofosforne kisline, c = 20 %
Dilute 23,5 ml of orthophosphoric acid (3.2) to 100 ml with water.
23,5 ml ortofosforne kisline (3.2) se razredči s 100 ml vode.
6-Methyl-2-heptylamine (1,5-dimethylhexylamine), w (w/w) = 99 %.
6-metil-2-heptilamin (1,5-dimetilheksilamin), m (m/m) = 99 %
Hydrochloric acid, density = 1,19 g/ml.
Klorovodikova kislina, gostota = 1,19 g/ml
Phosphate buffer solution, c = 0,01 mol/l.
Raztopina fosfatnega pufra, c = 0,01 mol/l
Transfer 5,0 ml of hydrochloric acid (3.6) into a 1000 ml graduated flask, make up to the mark with methanol (3.5) and mix.
5,0 ml klorovodikove kisline (3.6) se prenese v litrsko merilno bučko, dopolni se z metanolom (3.5) do oznake in premeša.
HPLC mobile phase, phosphate buffer-methanol solution 5 + 95 (V + V).
Mobilna faza za tekočinsko kromatografijo visoke ločljivosti (HPLC), raztopina fosfatnega pufra in metanola 5 + 95 (V + V)
Mix 5 ml of phosphate buffer solution (3.7) with 95 ml of methanol (3.5).
5 ml raztopine fosfatnega pufra (3.7) se zmeša s 95 ml metanola (3.5).
Lasalocid sodium standard substance with guaranteed purity, C34H53O8Na (sodium salt of a polyether monocarboxylic acid produced by Streptomyces lasaliensis), E763.
Lasalocid natrij standardna snov, zajamčene čistosti, C34H53O8Na (natrijeva sol polieter monokarboksilne kisline, proizvedena s Streptomyces lasaliensis), E763
Lasalocid sodium stock standard solution, 500 μg/ml
Osnovna standardna raztopina lasalocid natrija, 500 μg/ml
Weigh to the nearest 0,1 mg, 50 mg of lasalocid sodium (3.10) into a 100 ml graduated flask, dissolve in acidified methanol (3.8), make up to the mark with the same solvent and mix.
50 mg lasalocid natrija (3.10) se natehta na 0,1 mg natančno in prenese v 100-mililitrsko merilno bučko, raztopi se v nakisanem metanolu (3.8) ter z istim topilom dopolni do oznake in premeša.
This solution must be freshly prepared before use.
To raztopino je treba pripraviti svežo pred uporabo.
Lasalocid sodium intermediate standard solution, 50 μg/ml
Vmesna standardna raztopina lasalocid natrija, 50 μg/ml
Pipette 10,0 ml of stock standard solution (3.10.1) into a 100 ml graduated flask, make up to the mark with acidified methanol (3.8) and mix.
10,0 ml osnovne standardne raztopine (3.10.1) se odpipetira v 100-mililitrsko merilno bučko, z nakisanim metanolom (3.8) se dopolni do oznake in premeša.
Into a series of 50 ml graduated flasks transfer 1,0, 2,0, 4,0, 5,0 and 10,0 ml of the intermediate standard solution (3.10.2).
V serijo 50-mililitrskih merilnih bučk se prenese 1,0, 2,0, 4,0, 5,0 in 10,0 ml vmesne standardne raztopine (3.10.2).
Make up to the mark with acidified methanol (3.8) and mix.
Z nakisanim metanolom se dopolni do oznake (3.8) in premeša.
These solutions correspond to 1,0, 2,0, 4,0, 5,0 and 10,0 μg of lasalocid sodium per ml respectively.
Te raztopine ustrezajo 1,0, 2,0, 4,0, 5,0 in 10,0 μg lasalocid natrija na ml.
These solutions must be prepared freshly before use.
Te raztopine je treba pripraviti sveže pred uporabo.
Ultrasonic bath (or shaking water-bath) with temperature control.
Ultrazvočna kopel (ali stresalna vodna kopel) z nadzorom temperature
HPLC equipment with injection system, suitable for injecting volumes of 20 μl.
Oprema za tekočinsko kromatografijo visoke ločljivosti (HPLC) s sistemom za vbrizgavanje, primernim za vbrizgavanje volumnov 20 μl
Liquid chromatographic column 125 mm x 4 mm, reversed-phase C18, 5 μm packing or equivalent.
Kolona za tekočinsko kromatografijo 125 mm x 4 mm, reverzna faza C18, polnitev 5 μm ali enakovredna
Spectrofluorometer with variable wavelength adjustment of excitation and emission wavelengths.
Spektrofluorimeter s spremenljivo nastavitvijo valovnih dolžin ekscitacije in emisije
For the performance of the recovery test (5.1.2) a blank feed shall be analysed to check that neither lasalocid sodium nor interfering substances are present.
Za učinkovitost preskusa izkoristka (5.1.2) se z analizo slepe krme zagotovi, da niso prisotni niti lasalocid natrij niti moteče snovi.
The blank feed shall be similar in type to that of the sample and lasalocid sodium or interfering substances shall not be detected.
Slepa krma je podobne vrste kot vzorec in dokaže se odsotnost lasalocid natrija ali motečih snovi.
A recovery test shall be carried out by analysing the blank feed which has been fortified by addition of a quantity of lasalocid sodium, similar to that present in the sample.
Preskus izkoristka se izvede z analiziranjem slepe krme, ki se ji doda količina lasalocid natrija, podobna količini v vzorcu.
To fortify at a level of 100 mg/kg, transfer 10,0 ml of the stock standard (3.10.1) to a 250 ml conical flask and evaporate the solution to approximately 0,5 ml.
Za koncentracijo 100 mg/kg se 10,0 ml osnovnega standarda (3.10.1) prenese v 250-mililitrsko erlenmajerico, raztopina pa se upari do približno 0,5 ml.
Add 50 g of the blank feed, mix thoroughly and leave for 10 minutes mixing again several times before proceeding with the extraction step (5.2).
Doda se 50 g slepe krme, dobro se premeša in pusti 10 minut, preden se nadaljuje s postopkom ekstrakcije (5.2) pa se nekajkrat ponovno premeša.
In this case the sample to be analysed is fortified with a quantity of lasalocid sodium similar to that already present in the sample.
V tem primeru se vzorcu za analizo doda količina lasalocid natrija, podobna tisti v vzorcu.
This sample is analysed together with the unfortified sample and the recovery calculated by subtraction.
Ta vzorec se analizira skupaj z vzorcem brez dodanega lasalocid natrija, izkoristek pa se izračuna z odštevanjem.
Weigh to the nearest 0,01 g, from 5 g to 10 g of the sample into a 250 ml conical flask with stopper.
5–10 g vzorca se natehta na 0,01 g natančno in prenese v 250-mililitrsko erlenmajerico z zamaškom.
Add 100,0 ml of acidified methanol (3.8) by pipette.
S pipeto se doda 100,0 ml nakisanega metanola (3.8).
Stopper loosely and swirl to disperse.
Zamašek se rahlo zapre, erlenmajerica pa se zavrti, da se delci razpršijo.
Place the flask in an ultrasonic bath (4.1) at approximately 40 oC for 20 minutes, then remove and cool to room temperature.
Erlenmajerica se postavi v ultrazvočno kopel (4.1) pri približno 40 oC za 20 minut, nato se odstrani iz kopeli in ohladi na sobno temperaturo.
Allow to stand for about 1 hour until the suspended matter has settled, then filter an aliquot portion through a 0,45 μm membrane filter (4.2) into a suitable vessel.
Pusti se stati približno 1 uro, dokler se suspenzija ne usede, nato pa se alikvot filtrira skozi 0,45 μm membranski filter (4.2) v primerno posodo.
Weigh to the nearest 0,001 g about 2 g of the unground premix into a 250 ml graduated flask.
Približno 2 g nezmletega premiksa se natehta na 0,001 g natančno in prenese v 250-mililitrsko merilno bučko.
Add 100,0 ml of acidified methanol (3.8) and swirl to disperse.
Doda se 100,0 ml nakisanega metanola (3.8) in bučka se zavrti, da se delci razbijejo.
Place the flask and contents in an ultrasonic bath (4.1) at approximately 40 oC for 20 minutes, then remove and cool to room temperature.
Erlenmajerica z vsebino se postavi v ultrazvočno kopel (4.1) pri približno 40 oC za 20 minut, nato se odstrani iz kopeli in ohladi na sobno temperaturo.
Dilute to the mark with acidified methanol (3.8) and mix thoroughly.
Z nakisanim metanolom (3.8) se razredči do oznake in dobro premeša.
Allow to stand for 1 hour until the suspended matter has settled, then filter an aliquot portion through a 0,45 μm membrane filter (4.2).
Pusti se stati 1 uro, dokler se suspenzija ne obori, nato se alikvot filtrira skozi 0,45 μm membranski filter (4.2).
Dilute an appropriate volume of the clear filtrate with acidified methanol (3.8) to produce a final test solution containing about 4 μg/ml of lasalocid sodium.
Primeren volumen čistega filtrata se razredči z nakisanim metanolom (3.8), da nastane končna preskusna raztopina s koncentracijo približno 4 μg/ml lasalocid natrija.
The following conditions are offered for guidance; other conditions may be used, provided they yield equivalent results:
Naslednji pogoji se lahko uporabljajo kot smernice; lahko se uporabljajo tudi drugi pogoji, če zagotavljajo enakovredne rezultate:
Liquid chromatographic column (4.3.1):
Kolona za tekočinsko kromatografijo (4.3.1):
125 mm × 4 mm, reversed-phase C18, 5 μm packing or equivalent
125 mm × 4 mm, reverzna faza C18, polnitev 5 μm ali enakovredna
Mixture of phosphate buffer solution (3.7) and methanol (3.5), 5+95 (V+V)
Mešanica raztopine fosfatnega pufra (3.7) in metanola (3.5), 5+95 (V+V)
Detection wavelengths:
Detekcijska valovna dolžina:
Excitation:
ekscitacija:
Emission:
emisija
Check the stability of the chromatographic system, injecting the calibration solution (3.10.3) containing 4,0 μg/ml several times, until constant peak heights (or areas) and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizgavanjem raztopine za umerjanja s koncentracijo 4,0 μg/ml (3.10.3), dokler niso dosežene konstantne višine (ali površine) vrhov in konstantni retencijski časi.
Inject each calibration solution (3.10.3) several times and determine the mean peak heights (areas) for each concentration.
Vsaka raztopina za umerjanje (3.10.3) se večkrat vbrizga in za vsako koncentracijo se določijo srednje vrednosti višin (površin) vrhov.
Plot a calibration graph using the mean peak heights (areas) as the ordinates and the corresponding concentrations in μg/ml as the abscissae.
Umeritvena krivulja se pripravi tako, da se na ordinato nanesejo srednje vrednosti višin (površin) vrhov, na absciso pa ustrezne koncentracije v μg/ml.