natgillin/translations-raw huggingface dataset:
Remove 200 ml of the filtrate and evaporate to approximately half volume in order to eliminate most of the ethanol.
Odstrani se 200 ml filtrata in upari približno do polovice, da se odstrani skoraj ves etanol.
Transfer the evaporation residue quantitatively to a 200 ml volumetric flask using warm water, cool, bring up to volume with water, homogenise and filter if necessary.
Ostanek po uparevanju se s toplo vodo prenese v 200-mililitrsko merilno bučko, ohladi se, dopolni z vodo do oznake, homogenizira in po potrebi filtrira.
This solution will be used to determine the amount of reducing sugars and, after inversion, of total sugars.
Ta raztopina se uporabi za določanje količine reducirajočih sladkorjev, po inverziji pa celotnih sladkorjev.
Determination of reducing sugars
Določanje reducirajočih sladkorjev
Using a pipette, remove not more than 25 ml of the solution containing less than 60 mg of reducing sugars expressed as glucose.
Odpipetira se največ 25 ml raztopine, ki vsebuje manj kot 60 mg reducirajočih sladkorjev, izraženih kot glukoza.
If necessary, make up to 25 ml with distilled water and determine the content of reducing sugars by the Luff-Schoorl method.
Po potrebi se dopolni z destilirano vodo do 25 ml in določi vsebnost reducirajočih sladkorjev po Luff-Schoorlovi metodi.
The result is expressed as the percentage content of glucose in the sample.
Rezultat se izrazi kot masni delež glukoze v vzorcu.
Determination of total sugars after inversion
Določanje celotnih sladkorjev po inverziji
Using a pipette take 50 ml of the solution and transfer to a 100 ml volumetric flask.
50 ml raztopine se odpipetira v 100-mililitrsko merilno bučko.
Add a few drops of methyl orange solution (3.4) then, carefully and stirring continuously, add hydrochloric acid (3.5) until the liquid turns a definite red.
Doda se nekaj kapljic raztopine metiloranža (3.4), nato pa se previdno in ob neprestanem mešanju doda klorovodikova kislina (3.5), dokler tekočina na postane močno rdeča.
Add 15 ml of hydrochloric acid (3.6), immerse the flask in a fast boiling water bath and keep there for 30 minutes.
Doda se 15 ml klorovodikove kisline (3.6), bučka se potopi v skoraj vrelo vodno kopel in v njej pusti 30 minut.
Cool rapidly to approximately 20 oC and add 15 ml of sodium hydroxide solution (3.7).
Hitro se ohladi na približno 20 oC, nato pa se doda 15 ml raztopine natrijevega hidroksida (3.7).
Make up to 100 ml with water and homogenise.
Dopolni se z vodo do 100 ml in homogenizira.
Remove not more than 25 ml containing less than 60 mg of reducing sugars expressed as glucose.
Odpipetira se največ 25 ml raztopine, ki vsebuje manj kot 60 mg reducirajočih sladkorjev, izraženih kot glukoza.
The result is expressed as the percentage of glucose or, where appropriate, sucrose, by multiplying by the factor 0,95.
Rezultat se izrazi kot masni delež glukoze ali, kadar je to primerno, saharoze tako, da se pomnoži s faktorjem 0,95.
Titration by the Luff-Schoorl method
Titracija po Luff-Schoorlovi metodi
Using a pipette, take 25 ml of Luff-Schoorl reagent (3.8) and transfer to a 300 ml Erlenmeyer flask; add exactly 25 ml of the clarified sugar solution.
25 ml Luff-Schoorlovega reagenta (3.8) se odpipetira v 300-mililitrsko erlenmajerico; doda se natančno 25 ml zbistrene raztopine sladkorja.
Add 2 granules of pumice stone (3.13), heat, stirring by hand, over a free flame of medium height and bring the liquid to the boil in approximately two minutes.
Dodata se 2 zrni plovca (3.13) in med ročnim mešanjem se segreva nad prostim plamenom srednje višine tako, da raztopina zavre v približno dveh minutah.
Place the Erlenmeyer immediately on an asbestos-coated wire gauze with a hole approximately 6 cm in diameter under which a flame has been lit.
Erlenmajerica se takoj postavi na žično mrežico, prevlečeno z azbestom, ki ima odprtino s premerom 6 cm, pod katero je prižgan plamen.
The flame shall be regulated in such a way that only the base of the Erlenmeyer is heated.
Plamen se naravna tako, da se segreva le dno erlenmajerice.
Fit a reflux condenser to the Erlenmeyer flask.
Na erlenmajerico se namesti kondenzator povratnega toka.
Boil for exactly 10 minutes.
Pusti se vreti natančno 10 minut.
Cool immediately in cold water and after approximately five minutes titrate as follows:
Takoj se ohladi v mrzli vodi in čez približno 5 minut titrira na naslednji način:
Add 10 ml of potassium iodide solution (3.12) and immediately afterwards (carefully, because of the risk of abundant foaming), add 25 ml of sulphuric acid (3.11).
doda se 10 ml raztopine kalijevega jodida (3.12) in takoj nato (previdno zaradi možnosti močnega penjenja) 25 ml žveplove kisline (3.11).
Titrate with sodium thiosulphate solution (3.9) until a dull yellow colour appears, add the starch indicator (3.10) and complete titration.
Z raztopino natrijevega tiosulfata (3.9) se titrira do svetlorumene barve, doda se indikator škrob (3.10), nato pa se titracija zaključi.
Carry out the same titration on an accurately measured mixture of 25 ml of Luff-Schoorl reagent (3.8) and 25 ml of water, after adding 10 ml of potassium iodide solution (3.12) and 25 ml of sulphuric acid (3.11) without boiling.
Enaka titracija se izvede brez vretja z natančno izmerjeno mešanico 25 ml Luff-Schoorlovega reagenta (3.8) in 25 ml vode, potem ko se ji doda 10 ml raztopine kalijevega jodida (3.12) in 25 ml žveplove kisline (3.11).
Using the table establish the amount of glucose in mg which corresponds to the difference between the values of the two titrations, expressed in mg of sodium thiosulphate 0,1 mol/litre.
S pomočjo preglednice se določi količina glukoze v mg, ki ustreza razliki med vrednostma obeh titracij, izraženi v mg natrijevega tiosulfata 0,1 mol/l.
In the case of feed which are rich in molasses and other feed which are not particularly homogeneous, weigh out 20 g and place with 500 ml of water in a 1 litre volumetric flask.
Pri krmi, ki vsebuje veliko melase, in drugi krmi, ki ni zelo homogena, se natehta 20 g vzorca in s 500 ml vode prenese v litrsko merilno bučko.
Mix for one hour in the tumbler.
V mešalniku se meša 1 uro.
Clarify using Carrez 1 (3.2) and II (3.3) reagents as described under 5.1, this time however using four times the quantities of each reagent.
Raztopina se zbistri z uporabo reagentov Carrez I (3.2) in II (3.3), kakor je opisano v točki 5.1, vendar se uporabi štirikratna količina vsakega reagenta.
Bring up to volume with 80 % ethanol (v/v).
Bučka se dopolni z 80-odstotnim etanolom (v/v) do oznake.
Homogenise and filter.
Homogenizira se in filtrira.
Eliminate the ethanol as described under 5.1.
Etanol se odstrani, kakor je opisano v točki 5.1.
If there is no dextrinised starch, bring up to volume with distilled water.
Če ni dekstriniranega škroba, se dopolni do oznake z destilirano vodo.
In the case of molasses and feed materials which are rich in sugar and almost starch-free (carobs, dried beetroot cossettes etc.), weigh out 5 g, place in a 250 ml volumetric flask, add 200 ml of distilled water and mix in the tumbler for one hour, or more if necessary.
V primeru melas in posamične krme, ki vsebujejo veliko sladkorja in so skoraj brez škroba (rožiči, posušene rezine pese itd.), se natehta 5 g in prenese v 250-mililitrsko merilno bučko, doda se 200 ml destilirane vode in eno uro ali po potrebi več meša v mešalniku.
Clarify using Carrez I (3.2) and II (3.3) reagents as described under 5.1.
Raztopina se zbistri z uporabo reagentov Carrez I (3.2) in II (3.3), kakor je opisano v točki 5.1.
Bring up to volume with cold water, homogenise and filter.
Dopolni se z mrzlo vodo do oznake, homogenizira in filtrira.
In order to determine the amount of total sugars, continue as described under 5.3.
Za določanje količine celotnih sladkorjev se nadaljuje, kakor je opisano v točki 5.3.
In order to prevent foaming it is advisable to add (irrespective of the volume) approximately 1 ml of 3-methylbutan-l-ol (3.14) before boiling with Luff-Schoorl reagent.
Za preprečevanje penjenja je priporočljivo, da se pred vretjem z Luff-Schoorlovim reagentom doda (ne glede na volumen) približno 1 ml 3-metilbutan-l-ola (3.14).
The difference between the content of total sugars after inversion, expressed as glucose, and the content of reducing sugars, expressed as glucose, multiplied by 0,95, gives the percentage content of sucrose.
Razlika med vsebnostjo celotnih sladkorjev po inverziji, izraženih kot glukoza, in vsebnostjo reducirajočih sladkorjev, izraženih kot glukoza, pomnoženo z 0,95, je masni deleži saharoze.
In order to determine the content of reducing sugars, excluding lactose, two methods may be adopted:
Za določanje vsebnosti reducirajočih sladkorjev, razen laktoze, se lahko uporabljata dve metodi:
For an approximate calculation, multiply by 0,675 the lactose content established by a different method of analysis and subtract the result obtained from the content of reducing sugars.
za približen izračun se vsebnost laktoze, določena z drugo analitsko metodo, pomnoži z 0,675, dobljeni rezultat pa se odšteje od vsebnosti reducirajočih sladkorjev;
In both cases the amount of sugar present is determined by the Luff-Schoorl method and calculated in mg of glucose.
V obeh primerih se količina sladkorja določi z Luff-Schoorlovo metodo in izračuna v mg glukoze.
One of the values is subtracted from the other and the difference is expressed as a percentage of the sample.
Ena vrednost se odšteje od druge in razlika se izrazi kot masni delež vzorca.
The two volumes taken correspond, for each determination, to a sample of 250 mg.
Za vsako določanje oba uporabljena volumna ustrezata 250 mg vzorca.
In the first case 17 ml of sodium thiosulphate solution 0,1 mol/litre corresponding to 44,2 mg of glucose is consumed; in the second, 11 ml, corresponding to 27,6 mg of glucose.
V prvem primeru se porabi 17 ml raztopine natrijevega tiosulfata 0,1 mol/l, kar ustreza 44,2 mg glukoze, v drugem pa 11 ml, kar ustreza 27,6 mg glukoze.
The difference is 16,6 mg of glucose.
Razlika je 16,6 mg glukoze.
The content of reducing sugars (excluding lactose), calculated as glucose, is therefore:
Vsebnost reducirajočih sladkorjev (razen laktoze), izračunana kot glukoza, je zato:
Table of values for 25 ml of Luff-Schoorl reagent
Preglednica vrednosti za 25 ml Luff-Schoorlovega reagenta
ml of Na2 S2 O30,1 mol/litre, two minutes' heating, 10 minutes' boiling
ml Na2 S2 O30,1 mol/l, 2 minuti segrevanja, 10 minut vretja
Glucose, fructose invert sugars
Glukoza, fruktoza, sladkorji po inveriziji
difference
razlike
DETERMINATION OF LACTOSE
DOLOČANJE LAKTOZE
This method makes it possible to determine the level of lactose in feed containing more than 0,5 % of lactose.
Ta metoda omogoča določanje ravni laktoze v krmi, ki vsebuje več kot 0,5 % laktoze.
The sugars are dissolved in water.
Sladkorji se raztopijo v vodi.
The solution is subjected to fermentation by the yeast Saccharomyces cerevisiae which leaves the lactose intact.
Raztopina se fermentira s kvasovkami vrste Saccharomyces cerevisiae, ki ne spreminjajo laktoze.
After clarification and filtration the lactose content of the filtrate is determined by the Luff-Schoorl method.
Po zbistritvi in filtriranju se vsebnost laktoze v filtratu določi po Luff-Schoorlovi metodi.
Suspension of Saccharomyces cerevisiae: suspend 25 g of fresh yeast in 100 ml of water.
Suspenzija kvasovk vrste Saccharomyces cerevisiae: 25 g svežih kvasovk se raztopi v 100 ml vode.
The suspension will keep for a maximum period of one week in a refrigerator.
Suspenzija je ob hranjenju v hladilniku uporabna največ en teden.
Carrez solution I: dissolve in water 21,9 g of zinc acetate, Zn (CH3 COO)2 2H2O and 3 g of glacial acetic acid.
Raztopina Carrez I: 21,9 g cinkovega acetata Zn (CH3 COO)2 2H2O in 3 g ledoceta se raztopijo v vodi.
Stirring carefully, pour the citric acid solution (3.4.2) into the sodium carbonate solution (3.4.3).
med previdnim mešanjem se v raztopino natrijevega karbonata (3.4.3) vlije raztopina citronske kisline (3.4.2).
Add the copper sulphate solution (3.4.1) and make up to 1 litre with water.
Doda se raztopina bakrovega sulfata (3.4.1), nato pa se dopolni z vodo do 1 litra.
Copper sulphate solution: dissolve 25 g of copper sulphate Cu SO4 5H2O, free from iron, in 100 ml of water.
Raztopina bakrovega sulfata: v 100 ml vode se raztopi 25 g bakrovega sulfata (Cu SO4 × 5H2O), ki ne vsebuje železa.
Citric acid solution: dissolve 50 g of citric acid C6H8O7·H2O in 50 ml of water.
Raztopina citronske kisline: v 50 ml vode se raztopi 50 g citronske kisline (C6H8O7×H2O).
Solution of sodium thiosulphate 0,1 mol/litre.
Raztopina natrijevega tiosulfata, 0,1 mol/l
Boil for three minutes, leave to cool, and if necessary add 10 mg of mercuric iodide as a preservative.
Pusti se vreti 3 minute, nato pa se ohladi in po potrebi doda 10 mg živosrebrovega jodida kot konzervansa.
Water bath with thermostat set at 38-40 oC.
Vodna kopel s termostatom, nastavljenim na 38–40 oC
Weigh 1 g of the sample to the nearest mg and place this portion of the sample in a 100 ml volumetric flask.
1 g vzorca se natehta na 1 mg natančno in prenese v 100-mililitrsko merilno bučko.
Add 25 to 30 ml of water.
Doda se 25–30 ml vode.
Place the flask in a boiling water bath for 30 minutes and then cool to approximately 35 oC.
Bučka se za 30 minut postavi v vrelo vodno kopel, nato ohladi na približno 35 oC.
Add 5 ml of yeast suspension (3.1) and homogenise.
Doda se 5 ml suspenzije kvasovk (3.1) in homogenizira. Bučka se pusti 2 uri v vodni kopeli pri temperaturi 38–40 oC.
Leave the flask to stand for two hours in a water bath, at a temperature of 38-40o C. Cool to approximately 20 oC.
Ohladi se na približno 20 oC.
Add 2,5 ml of Carrez solution I (3.2) and stir for 30 seconds, then add 2,5 ml of Carrez solution II (3.3) and again stir for 30 seconds.
Doda se 2,5 ml raztopine Carrez I (3.2) in meša 30 sekund, nato se doda 2,5 ml raztopine Carrez II (3.3) in ponovno meša 30 sekund.
Make up to 100 ml with water, mix and filter.
Dopolni se z vodo do 100 ml, premeša in filtrira.
Using a pipette, remove an amount of filtrate which does not exceed 25 ml and which preferably contains from 40 to 80 mg of lactose and transfer it to a 300 ml Erlenmeyer flask.
Del filtrata, ki ne presega 25 ml in ki priporočljivo vsebuje 40 do 80 mg laktoze, se odpipetira in prenese v 300-mililitrsko erlenmajerico.
If necessary, make up to 25 ml with water.
Po potrebi se dopolni z vodo do 25 ml.
Carry out a blank test in the same way with 5 ml of yeast suspension (3.1).
Slepi preskus s 5 ml suspenzije kvasovk (3.1) se izvede pri enakih pogojih.
Determine the lactose content according to Luff-Schoorl, as follows: add exactly 25 ml of Luff-Schoorl reagent (3.4) and two granules of pumice stone (3.5).
Vsebnost laktoze se določi po Luff-Schoorlovi metodi na naslednji način: doda se natančno 25 ml Luff-Schoorlovega reagenta (3.4) in 2 zrni plovca (3.5).
Stir by hand-while heating over a free flame of medium height and bring the liquid to the boil in approximately two minutes.
Med ročnim mešanjem se segreva nad prostim plamenom srednje jakosti tako, da tekočina zavre v približno 2 minutah.
Add 10 ml of potassium iodide solution (3.6) and immediately afterwards (carefully, because of the risk of abundant foaming) add 25 ml of sulphuric acid (3.7).
doda se 10 ml raztopine kalijevega jodida (3.6) in takoj nato (previdno zaradi možnosti močnega penjenja) 25 ml žveplove kisline (3.7).
Titrate with sodium thiosulphate solution (3.8) until a dull yellow colour appears, add the starch indicator (3.9) and complete titration.
Z raztopino natrijevega tiosulfata (3.8) se titrira do svetlorumene barve, doda se indikator škrob (3.9), nato pa se titracija zaključi.
Carry out the same titration on an accurately measured mixture of 25 ml of Luff-Schoorl reagent (3.4) and 25 ml of water, after adding 10 ml of potassium iodide solution (3.6) and 25 ml of sulphuric acid (3.7) without boiling.
Enaka titracija se izvede brez vretja z natančno izmerjeno mešanico 25 ml Luff-Schoorlovega reagenta (3.4) in 25 ml vode, potem ko se ji doda 10 ml raztopine kalijevega jodida (3.6) in 25 ml žveplove kisline (3.7).
Using the attached table, establish the amount of lactose in mg which corresponds to the difference between the results of the two titrations, expressed in ml of sodium thiosulphate 0,1 mol/litre.
S pomočjo priložene preglednice se določi količina laktoze v mg, ki ustreza razliki med rezultatoma dveh titracij, izraženih v ml natrijevega tiosulfata 0,1 mol/l.
Express the result of anhydrous lactose as a percentage of the sample.
Rezultat se izrazi kot masni delež brezvodne laktoze v vzorcu.
For products containing more than 40 % of fermentable sugar, use more than 5 ml of yeast suspension (3.1).
Pri proizvodih, ki vsebujejo več kot 40 % fermentiranega sladkorja, se uporabi več kot 5 ml suspenzije kvasovk (3.1).
DETERMINATION OF STARCH
DOLOČANJE ŠKROBA
POLARIMETRIC METHOD
POLARIMETRIČNA METODA
This method makes it possible to determine the levels of starch and of high molecular weight starch degradation products in feed for the purpose of checking compliance with the declared energy value (provisions in Annex VII) and Council Directive 96/25/EC.2.
Ta metoda omogoča določanje ravni škroba in visokomolekularnih produktov razgradnje škroba v krmi za preverjanje skladnosti z označeno energijsko vrednostjo (določbe iz Priloge VII) in Direktivo Sveta 96/25/ES.2.
The method comprises two determinations.
Metoda vključuje dve določanji.
In the first, the sample is treated with dilute hydrochloric acid.
Pri prvi se vzorec obdela z razredčeno klorovodikovo kislino.
After clarification and filtration the optical rotation of the solution is measured by polarimetry.
Po zbistritvi in filtriranju se izmeri optična rotacija raztopine s polarimetrijo.
In the second, the sample is extracted with 40 % ethanol.
Pri drugi se vzorec ekstrahira s 40-odstotnim etanolom.
After acidifying the filtrate with hydrochloric acid, clarifying and filtering, the optical rotation is measured as in the first determination.
Po nakisanju filtrata s klorovodikovo kislino ter zbistritvi in filtriranju se izmeri optična rotacija tako kot pri prvem določanju.
The difference between the two measurements, multiplied by a known factor, gives the starch content of the sample.
Razlika med dvema meritvama, pomnožena z znanim faktorjem, je vsebnost škroba v vzorcu.
Hydrochloric acid, solution 25 % (w/w) density: 1,126 g/ml.
Klorovodikova kislina, 25-odstotna raztopina (m/v), gostota: 1,126 g/ml
Hydrochloric acid. solution 1,13 % (w/v)
Trikloroocetna kislina, 1,13-raztopina raztopina (m/v)
The concentration must be checked by titration using a sodium hydroxide solution 0,1 mol/litre in the presence of 0,1 % (w/v) methyl red in 94 % (v/v) ethanol.
Koncentracija se mora preveriti s titracijo z 0,1 mol/l raztopino natrijevega hidroksida v prisotnosti 0,1-odstotnega (m/v) metil rdečega v 94-odstotnem (v/v) etanolu.
For the neutralisation of 10 ml, 30,94 ml of NaOH 0,1 mol/litre is needed.
Za nevtralizacijo 10 ml je potrebno 30,94 ml NaOH 0,1 mol/l.
Carrez solution I: dissolve 21,9 g of zinc acetate Zn(CH3COO)2 2H2O and 3 g of glacial acetic acid in water.
Raztopina Carrez I: 21,9 g cinkovega acetata Zn(CH3COO)2 2H2O in 3 g ledoceta se raztopi v vodi.
Carrez solution II: dissolve 10,6 g of potassium ferrocyanide K4 Fe(CN)6 3H2O in water.
Raztopina Carrez II: 10,6 g kalijevega ferocianida K4(Fe(CN)6 3H2O se raztopi v vodi.
Ethanol, solution 40 % (v/v), density: 0,948 g/ml at 20 oC.
Etanol, 40-odstotna raztopina (v/v), gostota: 0,948 g/ml pri 20 oC
250 ml Erlenmeyer flask with standard ground-glass joint and with reflux condenser.
250-mililitrska erlenmajerica s standardnim brušenim vratom in kondenzatorjem povratnega toka
Polarimeter or saccharimeter.
Polarimeter ali saharometer
Crush the sample until it is fine enough for all of it to pass through a 0,5 mm round-meshed sieve.
Vzorec se zdrobi tako, da vsi delci preidejo skozi sito z okroglimi luknjicami premera 0,5 mm.
Determination of the total optical rotation (P or S) (see observation 7.1)
Določanje celotne optične rotacije (P ali S) (glej opombo 7.1)
Weigh 2,5 g of the crushed sample to the nearest mg and place in a 100 ml graduated flask.
2,5 g zdrobljenega vzorca se natehta na 1 mg natančno in prenese v 100-mililitrsko merilno bučko.
Add 25 ml of hydrochloric acid (3.2), shake to obtain even distribution of the test sample and add a further 25 ml of hydrochloric acid (3.2).
Doda se 25 ml klorovodikove kisline (3.2), pretrese se, da nastane enakomerna porazdelitev preskusnega vzorca, nato pa se doda nadaljnjih 25 ml klorovodikove kisline (3.2).
Immerse the flask in a boiling water bath shaking vigorously and steadily for the first three minutes to prevent the formation of agglomerates.
Bučka se potopi v vrelo vodno kopel in prve 3 minute močno in enakomerno stresa, da se prepreči nastanek aglomeratov.
The quantity of water in the water bath must be sufficient for the bath to remain at boiling point when the flask is introduced into it.
V vodni kopeli mora biti toliko vode, da kopel ostane na točki vrelišča, ko se vanjo potopi bučka.
The flask must not be taken out of the bath whilst being shaken.
Bučke se med stresanjem ne sme odstraniti iz kopeli.
After exactly 15 minutes, remove from the bath, add 30 ml of cold water and cool immediately to 20 oC.
Po natanko 15 minutah se bučka odstrani iz kopeli, doda se 30 ml hladne vode, nato pa se takoj ohladi na 20 oC.
Add 5 ml of Carrez solution I (3.3) and shake for approximately 30 seconds.
Doda se 5 ml Raztopine Carrez I (3.3) in stresa približno 30 sekund.
Then add 5 ml of Carrez solution II (3.4) and shake again for approximately 30 seconds.
Nato se doda 5 ml raztopine Carrez II (3.4) in ponovno stresa 30 sekund.
Make up to volume with water, mix and filter.
Dopolni se z vodo do oznake, premeša in filtrira.
If the filtrate is not perfectly clear (which is rare), repeat the determination using a larger quantity of Carrez solutions I and II, for example 10 ml.
Če filtrat ni popolnoma bister (kar je redko), se ponovi določanje z uporabo večje količine raztopin Carrez I in II, na primer 10 ml.
Measure the optical rotation of the solution in a 200 mm tube with the polarimeter or saccharimeter.
Optična rotacija raztopine v 200-milimetrski epruveti se izmeri s polarimetrom ali saharimetrom.
Determination of the optical rotation (P' or S') of substances soluble in 40 % ethanol
Določanje optične rotacije (P' ali S') snovi, ki so topne v 40-odstotnem etanolu
Weigh 5 g of the sample to the nearest mg, place in a 100 ml graduated flask and add about 80 ml of ethanol (3.5) (see observation 7.2).
5 g vzorca se natehta na 1 mg natančno, prenese v 100-mililitrsko merilno bučko in doda se približno 80 ml etanola (3.5) (glej opombo 7.2).
Leave the flask to stand for 1 hour at room temperature; during this time, shake vigorously on six occasions so that the test sample is thoroughly mixed with the ethanol.
Bučka se pusti stati 1 uro na sobni temperaturi; v tem času se šestkrat močno pretrese, da se preskusni vzorec temeljito premeša z etanolom.
Make up to volume with ethanol (3.5), mix and filter.
Dopolni se z etanolom (3.5) do oznake, premeša in filtrira.
Pipette 50 ml of the filtrate (corresponds to 2,5 g of the sample) into a 250 ml Erlenmeyer flask, add 2,1 ml of hydrochloric acid (3.1) and shake vigorously.
50 ml filtrata (kar ustreza 2,5 g vzorca) se odpipetira v 250-mililitrsko erlenmajerico, doda se 2,1 ml klorovodikove kisline (3.1) in močno pretrese.
Fit a reflux condenser to the Erlenmeyer flask and immerse the latter in a boiling water bath.
Erlenmajerica se spoji s kondenzatorjem povratnega toka in potopi v vrelo vodno kopel.
After exactly 15 minutes, remove the Erlenmeyer flask from the bath, transfer the contents to a 100 ml graduated flask, rinsing with a little cold water, and cool to 20 oC.
Po natanko 15 minutah se erlenmajerica odstrani iz kopeli, vsebina se prenese v 100-mililitrsko merilno bučko, spere se z malo hladne vode in ohladi na 20 oC.