natgillin/translations-raw huggingface dataset:
The standard solution is diluted with citrate buffer to give peak areas in the middle of the range.
Standardna raztopina se razredči s citratnim pufrom, da je površina vrha na sredini tega območja.
Where high performance liquid chromatographic equipment is used to analyse the hydrolysates, the experimental conditions must be optimised in accordance with the manufacturer's recommendations.
Kadar se za analizo hidrolizatov uporablja oprema za tekočinsko kromatografijo visoke ločljivosti, se morajo eksperimentalni pogoji optimizirati v skladu s priporočili proizvajalca.
By applying the method to feed containing more than 1 % chloride (concentrate, mineral feeds, supplementary feeds) underestimation of methionine could occur and special treatment has to be done.
Pri uporabi metode za krmo, ki vsebuje več kot 1 % klorida (koncentrat, mineralna krma, nadomestna krma), se lahko pojavi prenizka vrednost vsebnosti metionina in je zato potrebna posebna obdelava.
DETERMINATION OF TRYPTOPHAN
DOLOČANJE TRIPTOFANA
The method makes the determination possible of the total and free tryptophan in feed.
Ta metoda omogoča določanje celotnega in prostega triptofana v krmi.
It does not distinguish between D- and L- forms.
Ne omogoča pa ločevanja med oblikama D in L.
For the determination of the total tryptophan, the sample is hydrolysed under alkaline conditions with saturated barium hydroxide solution and heated to 110 oC for 20 hours.
Za določanje celotnega triptofana se vzorec hidrolizira pri bazičnih pogojih z nasičeno raztopino barijevega hidroksida in 20 ur segreva do 110 oC.
After hydrolysis internal standard is added.
Po hidrolizi se doda notranji standard.
For the determination of free tryptophan, the sample is extracted under mild acidic conditions in the presence of internal standard.
Za določanje prostega triptofana se vzorec ekstrahira pri rahlo kislih pogojih v prisotnosti notranjega standarda.
The tryptophan and the internal standard in the hydrolysate or in the extract are determined by HPLC with fluorescence detection.
Triptofan in notranji standard v hidrolizatu ali ekstraktu se določata s HPLC s fluorescenčno detekcijo.
Double distilled water or water of equivalent quality must be used (conductivity < 10 μS/cm).
Uporabljati je treba dvakrat destilirano vodo ali vodo enake kakovosti (prevodnost < 10 μS/cm).
Standard substance: tryptophan (purity/content ≥ 99 %) dried under vacuum over phosphorous pentoxide.
Standardna snov: triptofan (čistoča/vsebnost ≥ 99 %), osušen pod vakuumom nad fosforjevim pentoksidom
Internal standard substance: α-methyl-tryptophan (purity/content ≥ 99 %), dried under vacuum over phosphorous pentoxide.
Snov, ki je notranji standard: α-metil-triptofan (čistoča/vsebnost ≥ 99 %), osušen pod vakuumom nad fosforjevim pentoksidom.
Barium hydroxide octa-hydrate (care shall be taken not to expose the Ba(OH)2 .8 H2O excessively to air in order to avoid formation of BaCO3, which could disturb the determination) (see observation 9.3).
Barijev hidroksid oktahidrat (paziti je treba, da Ba(OH)2 .8 H2O ni pretirano izpostavljen zraku, da se ne bi tvoril BaCO3, ki lahko moti določanje) (glej opombo 9.3).
Ortho-phosphoric acid, w (w/w) = 85 %.
Ortofosforna kislina, m (m/m) = 85 %
Hydrochloric acid, ρ201,19 g/ml.
Klorovodikova kislina, ρ201,19 g/ml
Methanol, equivalent to HPLC grade.
Metanol, za HPLC
Dissolve 40,0 g NaOH (3.5) in water and make up to 1 litre with water (3.1).
40,0 g NaOH (3.5) se raztopi v vodi in dopolni z vodo do 1 litra (3.1).
Hydrochloric acid, c = 6 mol/l:
Klorovodikova kislina, c = 6 mol/l:
Take 492 ml HCl (3.7) and make up to 1 litre with water.
492 ml HCl (3.7) se dopolni z vodo do 1 litra.
Hydrochloric acid, c = 1 mol/l:
Klorovodikova kislina, c = 1 mol/l:
Take 82 ml HCl (3.7) and make up to 1 litre with water.
82 ml HCl (3.7) se dopolni z vodo do 1 litra.
Hydrochloric acid, c = 0,1 mol/l:
Klorovodikova kislina, c = 0,1 mol/l:
Take 8,2 ml HCl (3.7) and make up to 1 litre with water.
8,2 ml HCl (3.7) se dopolni z vodo do 1 litra.
Ortho-phosphoric acid, c = 0,5 mol/l:
Ortofosforna kislina, c = 0,5 mol/l:
Take 34 ml ortho-phosphoric acid (3.6) and make up to 1 litre with water (3.1).
34 ml ortofosforne kisline (3.6) se dopolni z vodo (3.1) do 1 litra.
Concentrated solution of tryptophan (3.2), c = 2,50 μmol/ml:
Koncentrirana raztopina triptofana (3.2), c = 2,50 μmol/ml:
In a 500 ml volumetric flask dissolve 0,2553 g tryptophan (3.2) in hydrochloric acid (3.13) and make up to the mark with hydrochloric acid (3.13).
v 500-mililitrski merilni bučki se raztopi 0,2553 g triptofana (3.2) v klorovodikovi kislini (3.13) in dopolni s klorovodikovo kislino (3.13) do oznake.
Store at - 18 oC for a maximum of 4 weeks.
Pri –18 oC se shrani največ 4 tedne.
Concentrated internal standard solution, c = 2,50 μmol/ml:
Koncentrirana raztopina notranjega standarda, c = 2,50 μmol/ml:
In a 500 ml volumetric flask dissolve 0,2728 g α-methyl-tryptophan (3.3) in hydrochloric acid (3.13) and make up to the mark with hydrochloric acid (3.13).
v 500-mililitrski merilni bučki se raztopi 0,2728 g α-metil-triptofana (3.3) v klorovodikovi kislini (3.13) in dopolni se s klorovodikovo kislino (3.13) do oznake.
Calibration standard solution of tryptophan and internal standard:
Standardna raztopina triptofana in notranjega standarda za umerjanje:
Take 2,00 ml concentrated solution of tryptophan (3.15), and 2,00 ml of concentrated internal standard (α-methyl-tryptophan) solution (3.16).
pripravi se 2,00 ml koncentrirane raztopine triptofana (3.15) in 2,00 ml koncentrirane raztopine notranjega standarda (α-metil-triptofan) (3.16).
Dilute with water (3.1) and methanol (3.8) to approximately the same volume and to approximately the same concentration of methanol (10 %-30 %) as the finished hydrolysate.
Razredči se z vodo (3.1) in metanolom (3.8) na približno enak volumen in približno enako koncentracijo metanola (10–30 %) kot pri končnem hidrolizatu.
This solution must be prepared freshly before use.
Ta raztopina se mora pripraviti sveža pred uporabo.
Protect from direct sunlight during preparation.
Med pripravo jo je treba zaščititi pred neposredno sončno svetlobo.
1,1,1-trichloro-2-methyl-2-propanol.
1,1,1-trikloro-2-metil-2-propanol
Ethanolamine w (w/w) > 98 %.
Etanolamin, m (m/m) > 98 %
Solution of 1 g 1,1,1-trichloro-2-methyl-2-propanol (3.19) in 100 ml methanol (3.8).
1 g 1,1,1-trikloro-2-metil-2-propanola (3.19) se raztopi v 100 ml metanola (3.8).
Make up to 1000 ml with water (3.1).
Dopolni se z vodo do 1000 ml (3.1).
HPLC equipment with a spectrofluorometric detector.
Oprema HPLC s spektrofluorometričnim detektorjem
Liquid chromatographic column, 125 mm x 4 mm, C18, 3 μm packing, or equivalent.
Kolona za tekočinsko kromatografijo, 125 mm x 4 mm C18, polnitev 3 μm ali enakovredna
Polypropylene flask, capacity 125 ml, with wide neck and screw cap.
Bučka iz polipropilena, volumna 125 ml, s širokim vratom in zamaškom z navojem
Membrane filter, 0,45 μm.
Membranski filter, 0,45 μm
Autoclave, 110 (± 2) oC, 1,4 (±0,1) bar.
Avtoklav, 110 (± 2) oC, 1,4 (±0,1) bar
Vortex mixer.
Vorteks mešalnik
Samples with high fat content shall be extracted with light petroleum (3.9) prior to grinding.
Vzorci z visoko vsebnostjo maščobe se pred drobljenjem ekstrahirajo s petroletrom (3.9).
Determination of free tryptophan (extract)
Določanje prostega triptofana (ekstrakt)
Weigh to the nearest 1 mg an appropriate amount (1-5 g) of the prepared sample (5.1), into a conical flask.
Primerna količina (1–5 g) pripravljenega vzorca (5.1) se natehta na 1 mg natančno in prenese v erlenmajerico.
Add 100,0 ml hydrochloric acid, (3.13) and 5,00 ml concentrated internal standard solution (3.16).
Doda se 100,0 ml klorovodikove kisline (3.13) in 5,00 ml koncentrirane raztopine notranjega standarda (3.16).
Shake or mix for 60 min. using a mechanical shaker or a magnetic stirrer (4.7).
Z mehanskim stresalnikom ali magnetnim mešalom (4.7) se stresa ali meša 60 minut.
Allow the sediment to settle and pipette 10,0 ml of the supernatant solution into a beaker.
Pusti se, da se usedlina usede na dno, nato pa se odpipetira 10,0 ml supernatanta v čašo.
Add sufficient methanol (3.8) to give a concentration of between 10 % and 30 % of methanol in the final volume.
Doda se dovolj metanola (3.8), da je v končnem volumnu koncentracija metanola 10–30 %.
Transfer to a volumetric flask of appropriate volume and dilute with water to a volume necessary for the chromatography (approx. the same volume as the calibration standard solution (3.17)).
Prenese se v merilno bučko primernega volumna in razredči z vodo na volumen, potreben za kromatografijo (približno enak volumen kot pri standardni raztopini za umerjanje (3.17)).
Filter a few ml of the solution through a 0,45 μm membrane filter (4.5) before injection on the HPLC column.
Pred vbrizganjem na HPLC kolono se nekaj ml raztopine prefiltrira skozi 0,45 μm membranski filter (4.5).
Protect standard solution and extracts against direct sunlight.
Standardna raztopina in ekstrakti se zaščitijo pred neposredno sončno svetlobo.
If it is not possible to analyse the extracts the same day, the extracts may be stored at 5 oC for a maximum of 3 days.
Če ni možno analizirati ekstraktov istega dne, se lahko hranijo največ 3 dni pri 5 oC.
Determination of total tryptophan (hydrolysate)
Določanje celotnega triptofana (hidrolizat)
Weigh to the nearest 0,2 mg from 0,1 to 1 g of the prepared sample (5.1) into the polypropylene flask (4.4).
0,1–1 g pripravljenega vzorca (5.1) se natehta na 0,2 mg natančno in prenese v bučko iz polipropilena (4.4).
The weighed sample portion shall have a nitrogen content of about 10 mg.
Vsebnost dušika v natehtanem delu vzorca je približno 10 mg.
Add 8,4 g barium hydroxide octa-hydrate (3.4) and 10 ml water.
Doda se 8,4 mg barijevega hidroksid oktahidrata (3.4) in 10 ml vode.
Mix on a vortex mixer (4.8) or magnetic stirrer (4.7).
Meša se na vorteks mešalniku (4.8) ali z magnetnim mešalom (4.7).
Leave the teflon coated magnet in the mixture.
S teflonom prevlečeni magnet se pusti v mešanici.
Wash down the walls of the vessel with 4 ml water.
Stene posode se sperejo s 4 ml vode.
Put on the screw cap and close the flask loosely.
Zapre se z zamaškom z navojem, bučka pa se rahlo zapre.
Before opening the autoclave reduce the temperature to just under 100 oC.
Preden se avtoklav odpre, se temperatura zmanjša nekoliko pod 100 oC.
In order to avoid crystallisation of Ba(OH)2 · 8 H2O, add to the warm mixture 30 ml water which is at room temperature.
Da se prepreči kristalizacija Ba(OH)2 ×8 H2O, se topli mešanici doda 30 ml vode sobne temperature.
Shake or stir gently.
Rahlo se pretresa ali meša.
Add 2,00 ml concentrated internal standard (α-methyl-tryptophan) solution (3.16).
Doda se 2,00 ml koncentrirane raztopine notranjega standarda (α-metil-triptofana) (3.16).
Cool the vessels on water/ice bath for 15 minutes.
Posode se 15 minut hladijo na vodni/ledeni kopeli.
Then, add 5 ml ortho-phosphoric acid (3.14).
Nato se doda 5 ml ortofosforne kisline (3.14).
Keep the vessel in the cooling bath and neutralise with HCl (3.11) whilst stirring and adjust the pH to 3,0 using HCl (3.12).
Posoda se zadrži v hladilni kopeli, med mešanjem nevtralizira s HCl (3.11), pH pa se s HCl (3.12) uravna na 3,0.
Add sufficient methanol to give a concentration of between 10 % and 30 % of methanol in the final volume.
Doda se dovolj metanola, da je koncentracija metanola v končnem volumnu 10–30 %.
Transfer to a volumetric flask of appropriate volume and dilute with water to the defined volume necessary for the chromatography (for example 100 ml).
Prenese se v merilno bučko primernega volumna in razredči z vodo do volumna, potrebnega za kromatografijo (na primer 100 ml).
The addition of methanol shall not cause precipitation.
Dodatek metanola ne povzroči obarjanja.
Protect standard solution and hydrolysates against direct sunlight.
Standardna raztopina in hidrolizati se zaščitijo pred neposredno sončno svetlobo.
If it is not possible to analyse the hydrolysates the same day, they may be stored at 5 oC for a maximum of 3 days.
Če ni možno analizirati ekstraktov istega dne, se lahko hranijo največ 3 dni pri 5 oC.
HPLC determination
Določanje s HPLC
The following conditions for isocratic elution are offered for guidance; other conditions may be used, provided they yield equivalent results (see also observations 9.1 and 9.2):
Za izokratsko eluiranje se lahko kot smernica uporabljajo naslednji pogoji; lahko se uporabljajo tudi drugi pogoji, če zagotavljajo enakovredne rezultate (glej tudi opombi 9.1 in 9.2).
Liquid chromatographic column (4.2):
Kolona za tekočinsko kromatografijo (4.2):
125 mm x 4 mm, C18, 3 μm packing or equivalent
125 mm × 4 mm, C18, polnitev 3 μm ali enakovredna
Column temperature:
Temperatura kolone:
Room temperature
sobna temperatura
Mobile phase (3.22):
Mobilna faza (3.22):
Make up to 1000 ml with water (3.1)
Dopolni se z vodo (3.1) do 1000 ml.
Flow rate:
Hitrost pretoka:
Total run time:
Skupni čas izvedbe:
approx. 34 min.
približno 34 min
Detection wavelength:
Detekcijska valovna dolžina:
excitation: 280 nm, emission: 356 nm.
ekscitacija: 280 nm, emisija: 356 nm.
Injection volume
Volumen, ki se vbrizga:
The amount of tryptophane (X), in g per 100g sample, is calculated as follows:
Količina triptofana (X), izražena v g na 100 g vzorca, se izračuna po naslednji formuli:
A peak area of internal standard, calibration standard solution (3.17)
A površina vrha notranjega standarda, standardne raztopina za umerjanje (3.17);
B peak area of tryptophan, extract (5.2) or hydrolysate (5.3)
B površina vrha triptofana, ekstrakta (5.2) ali hidrolizata (5.3);
volume in ml (2 ml) of concentrated tryptophan solution (3.15) added to the calibration solution (3.17)
volumen, v ml (2 ml), koncentrirane raztopine triptofana (3.15), dodane raztopini za umerjanje (3.17);
c concentration in μmol/ml (= 2,50) of concentrated tryptophan solution (3.15) added to calibration solution (3.17)
c koncentracija, v μmol/ml (= 2,50), koncentrirane raztopine triptofana (3.15), dodane raztopini za umerjanje (3.17);
volume in ml of concentrated internal standard solution (3.16) added at the extraction (5.2) (= 5,00 ml) or to the hydrolysate (5.3) (= 2,00 ml)
volumen, v ml, koncentrirane raztopine notranjega standarda (3.16), dodane pri ekstrakciji (5.2) (= 5,00 ml) ali hidrolizatu (5.3) (= 2,00 ml);
C peak area of internal standard, extract (5.2) or hydrolysate (5.3)
C površina vrha notranjega standarda, ekstrakta (5.2) ali hidrolizata (5.3);
D peak area of tryptophan, calibration standard solution (3.17)
D površina vrha triptofana, standardne raztopine za umerjanje (3.17);
volume in ml (= 2,00 ml) of concentrated internal standard solution (3.16) added to calibration standard solution (3.17)
volumen, v ml (= 2,00 ml), koncentrirane raztopine notranjega standarda (3.16), dodane standardni raztopini za umerjanje (3.17);
m sample weight in g (corrected to original weight if dried and/or defatted)
m masa vzorca v g (popravljena na prvotno maso, če je vzorec posušen in/ali razmaščen);
M molar weight of tryptophan (= 204,23 g/mol)
M molska masa triptofana (= 204,23 g/mol).
The difference between the results of two parallel determinations carried out on the same sample must not exceed 10 % relative to the highest result.
Razlika med rezultatoma dveh vzporednih določanj, izvedenih na istem vzorcu, ne sme presegati 10 % najvišjega rezultata.
Results of a collaborative study
Rezultati medlaboratorijske študije
An EC collaborative study (4th intercomparison) was arranged in which three samples were analysed by up to 12 laboratories to certify the method for hydrolysis.
Organizirana je bila medlaboratorijska študija ES (četrta medlaboratorijska primerjava), pri kateri je 12 laboratorijev analiziralo 3 vzorce, da se potrdi metoda za hidrolizo. Vsak vzorec je bil analiziran petkrat.
Replicate (5) analyses were performed on each sample.
Vsak vzorec je bil analiziran petkrat.
The results are given in the following table:
Rezultati so navedeni v spodnji preglednici:
Pig feed
Prašičja krma
Pig feed supplemented with L-tryptophan
Prašičja krma z dodanim L-triptofanom
Sample 3
Vzorec 3
Feed concentrate for pigs
Koncentrat prašičje krme
Mean [g/kg]
Srednja vrednost [g/kg]
L number of laboratories submitting results
L število laboratorijev, ki so predložili rezultate;
n number of single results retained eliminating outliers (identified by Cochran, Dixon outlier test)
n število uporabljenih posameznih rezultatov, razen izjem (identificiranih s testom za izjeme po Cochranu in Dixonu);
standard deviation of repeatability
standardni odmik ponovljivosti;
standard deviation of reproducibility
standardni odmik obnovljivosti;
r repeatability
r ponovljivost;
R reproducibility
R obnovljivost;
coefficient of variation of repeatability, %
koeficient variacije ponovljivosti v %;
coefficient of variation of reproducibility, %
koeficient variacije obnovljivosti v %.
Another EC collaborative study (3rd intercomparison) was arranged in which two samples were analysed by up to 13 laboratories to certify the method for extraction of free tryptophan.
Organizirana je bila še ena medlaboratorijska študija ES (tretja medlaboratorijska primerjava), v kateri je 13 laboratorijev analiziralo 2 vzorca, da se potrdi metoda za ekstrakcijo prostega triptofana. Vsak vzorec je bil analiziran petkrat.
Sample 4
Vzorec 4
Wheat and soya mixture
Mešanica pšenice in soje
Sample 5
Vzorec 5
Wheat and soya mixture (= sample 4) with added tryptophan (0,457g/kg1)
Mešanica pšenice in soje (= vzorec 4) z dodanim triptofanom (0,457 g/kg1)
n number of single results retained after eliminating outliers (identified by Cochran, Dixon outlier test)
n število uporabljenih posameznih rezultatov, po izločitvi izjem (identificiranih s testom za izjeme po Cochranu in Dixonu);
Another EC intercomparison study was arranged in which four samples were analysed by up to 7 laboratories with the aim of a tryptophan certification for hydrolysis.
Organizirana je bila še ena medlaboratorijska študija ES, v kateri je do 7 laboratorijev analiziralo 4 vzorce, da se potrdi metoda za hidrolizo triptofana.
The results are given below.
Rezultati so prikazani spodaj.
Mixed pig feed
Mešana prašičja krma
Low fat fish meal
Ribja moka z nizko vsebnostjo maščob
Soybean meal
Sojina moka
Following special chromatographic conditions may give better separation between tryptophan and α-methyl-tryptophan.
Ločevanje triptofana in α-metil-triptofana lahko izboljšajo naslednji posebni kromatografski pogoji.
Isocratic elution followed by gradient column cleaning:
Izokratsko eluiranje, ki mu sledi gradientno čiščenje kolone:
Liquid chromatographic column:
Kolona za tekočinsko kromatografijo:
125 mm x 4 mm, C18, 5 μm packing or equivalent
125 mm x 4 mm, C18, polnitev 5 μm ali enakovredna
Mobile phase:
Mobilna faza:
0,01 mol/l KH2PO4/méthanol, 95+5 (V+V).
0,01 mol/l KH2PO4/Metanol, 95+5 (V+V).
Gradient program:
Gradientni program:
approx. 33 min.
približno 33 min
The chromatography will vary according to the type of HPLC and column packing material used.
Kromatografija se spreminja glede na vrsto HPLC in uporabljeni material za polnitev kolone.
The chosen system must be capable of giving baseline separation between the tryptophan and the internal standard.
Izbrani sistem mora zagotavljati ločevanje bazne linije triptofana in notranjega standarda.
Moreover it is important that degradation products are well separated from the tryptophan and the internal standard.
Poleg tega je pomembno, da se produkti razgradnje dobro ločijo od triptofana in notranjega standarda.
Hydrolysates without internal standard shall be run in order to check the base line under the internal standard for impurities.
Izvede se postopek s hidrolizatom brez notranjega standarda, da se preveri bazna linija notranjega standarda za nečistoče.
It is important that the run time is sufficiently long for the elution of all the degradation products, otherwise late eluting peaks may interfere with subsequent chromatographic runs.
Pomembno je, da je čas izvedbe dovolj dolg, da se eluirajo vsi produkti razgradnje, sicer lahko zapozneli vrhovi eluiranja motijo nadaljnje kromatografiranje.
In the range of operation, the chromatographic system shall give linear response.
Kromatografski sistem se v delovnem območju odziva linearno.
The linear response shall be measured with a constant (the normal) concentration of the internal standard and varying concentrations of tryptophan.
Linearni odziv se izmeri s konstantno (običajno) koncentracijo notranjega standarda in različnimi koncentracijami triptofana.
It is of importance that the size of both the tryptophan and internal standard peaks are within the linear range of the HPLC/fluorescence system.
Zelo pomembno je, da so velikosti vrhov triptofana in notranjega standarda v linearnem območju HPLC/fluorescenčnega sistema.
If either the tryptophan and/or the internal standard peak(s) is (are) too small or too high the analysis shall be repeated with another sample size and/or a changed final volume.
Če so vrhovi triptofana in/ali notranjega standarda prenizki ali previsoki, se analiza ponovi z drugačno količino vzorca in/ali drugačnim končnim volumnom.
Barium hydroxide
Barijev hidroksid
With age barium hydroxide becomes more difficult to dissolve.
Topnost barijevega hidroksida se s staranjem zmanjša.
This results in an unclear solution for the HPLC determination, which may produce low results for tryptophan.
Posledica tega je motna raztopina za določitev HPLC, kar lahko povzroči nizke rezultate za triptofan.
DETERMINATION OF CRUDE OILS AND FATS
DOLOČANJE SUROVIH OLJ IN MAŠČOB
This method is for the determination of crude oils and fats in feed.
Ta metoda omogoča določanje vsebnosti surovih olj in maščob v krmi.
It does not cover the analysis of oil seeds and oleaginous fruit.
Ne omogoča analize oljnih semen in oljnatega sadja.
The use of the two procedures described below depends on the nature and composition of the feed and the reason for carrying out the analysis.
Vrsta in sestava krme ter namena izvajanja analize vplivata na uporabo spodaj opisanih postopkov.
Procedure A — Directly extractable crude oils and fats
Postopek A – Surova olja in maščobe, ki se ekstrahirajo neposredno
This method is applicable to feed materials of plant origin, except those included within the scope of Procedure B.
Ta metoda se uporablja za krmo rastlinskega izvora, razen tiste, ki je zajeta v področju uporabe postopka B.
Procedure B — Total crude oils and fats
Postopek B – Celotna surova olja in maščobe
This method is applicable to feed materials of animal origin and to all compound feeds.
Ta metoda se uporablja za krmo živalskega izvora in za vse krmne mešanice.
It is to be used for all materials from which the oils and fats cannot be completely extracted without prior hydrolysis (e.g. glutens, yeast, potato proteins and products subjected to processes such as extrusion, flaking and heating).
Uporablja se za vso krmo, iz katere ni mogoče povsem ekstrahirati olj in maščob brez predhodne hidrolize (npr. gluteni, kvas, krompirjeve beljakovine in izdelki, pri katerih se izvaja izrivanje, kosmičenje in segrevanje).
In all cases where a higher result is obtained by using Procedure B than by Procedure A, the result obtained by Procedure B shall be accepted as the true value.
Vedno kadar je rezultat pri postopku B višji od rezultata pri postopku A, se kot prava vrednost sprejme rezultat, dobljen pri postopku B.
Procedure A
Postopek A
The sample is extracted with light petroleum.
Vzorec se ekstrahira s petroletrom.
The solvent is distilled off and the residue dried and weighed.
Topilo se oddestilira, ostanek pa osuši in stehta.
Procedure B
Postopek B
The sample is treated under heating with hydrochloric acid.
Vzorec se med segrevanjem obdela s klorovodikovo kislino.
The mixture is cooled and filtered.
Mešanica se ohladi in filtrira.
The residue is washed and dried and submitted to the determination according to Procedure A.
Ostanek se spere in osuši, nato pa se izvede določanje v skladu s postopkom A.
Light petroleum, boiling range: 40 to 60 oC.
Petroleter, vrelišče 40–60 oC.
The bromine value must be less than 1 and the residue on evaporation less than 2 mg/100 ml.
Bromno število mora biti nižje od 1 in ostanek pri uparevanju nižji od 2 mg/100 ml.
Sodium sulfate, anhydrous.
Natrijev sulfat, brezvodni