natgillin/translations-raw huggingface dataset:
I, the undersigned, certify that the animal/s described above meet/s the following requirements:
Spodaj podpisani potrjujem, da živali, opisane zgoraj, izpolnjujejo naslednje zahteve:
it/they has/have been examined today and show/s no clinical sign of disease;
so bile danes pregledane in ne kažejo kliničnih znakov bolezni;
it/they is/are not intended for slaughter under a national programme of contagious or infectious disease eradication;
niso namenjene za zakol po nacionalnem programu za izkoreninjanje kužnih ali infekcijskih bolezni;
it/they does/do not come from the territory or part of the territory of a Member State, which is the subject of restrictions for reasons of African horse sickness;]
ne izvirajo z ozemlja ali dela ozemlja države članice, za katerega zaradi konjske kuge veljajo omejitve;]
it/they come/s from the territory or part of the territory of a Member State, which is the subject of restrictions for reasons of African horse sickness, have remained for at least 40 days prior to dispatch in the vector proved quarantine station of … and has/have undergone a test for the detection of antibodies to the African horse sickness virus as described in Annex IV to Directive 2009/156/EC carried out simultaneously on blood samples taken on two occasions with an interval of between 21 and 30 days on … (insert date) and during the 10 days prior to dispatch on … (insert date);
izvirajo z ozemlja ali dela ozemlja države članice, za katerega zaradi konjske kuge veljajo omejitve, so bile vsaj 40 dni pred nakladanjem v karantenskem centru z dokazano zaščito pred vektorji … in imajo opravljen test za odkrivanje protiteles proti virusu konjske kuge, kot je opisan v Prilogi IV k Direktivi 2009/156/ES, ki je bil izveden istočasno na dveh krvnih vzorcih, odvzetih v razmiku 21 do 30 dni, in sicer dne … (vstaviti datum) ter dne … (vstaviti datum), pri čemer je bil drugi vzorec odvzet največ 10 dni pred nakladanjem;
either (3)
bodisi (3)
[with negative result in each case if it/they was/were not vaccinated against African horse sickness;]
[z negativnim rezultatom v obeh primerih, če niso bile cepljene proti konjski kugi;]
[without increase in antibody count, if it/they was/were vaccinated against African horse sickness;]
[brez povečanja števila protiteles, če so bile cepljene proti konjski kugi;]
it/they is/are not vaccinated against African horse sickness;]
niso bile cepljene proti konjski kugi;]
it/they was/were vaccinated against African horse sickness on … (insert date),
so bile cepljene proti konjski kugi dne … (vstaviti datum),
[at least two months prior to certification;]
[vsaj dva meseca pred izdajo spričevala;]
[at least two months prior to entry into the quarantine station;]
[vsaj dva meseca pred vstopom v karantenski center;]
it/they does/do not come from (a) holding(s) which was/were subject to prohibition order(s) for animal health reasons which laid down at least of one the following conditions:
ne izvirajo iz gospodarstva, za katerega je veljala prepoved zaradi zdravstvenega stanja živali, v kateri je bil določen vsaj eden od naslednjih pogojev:
[not all the animals on the holding of species susceptible to the diseases mentioned in points (a) to (g) hereinafter were slaughtered and the prohibition lasted at least for:
[niso bile zaklane vse živali na gospodarstvu iz vrst, dovzetnih za bolezni iz točk (a) do (g) v nadaljevanju, prepoved pa je veljala vsaj:
in the case of equidae suspected of having contracted dourine,
za kopitarje s sumom na spolno kugo konj:
[six months beginning on the date of the last actual or possible contact with a sick or infected with Trypanosoma equiperdum animal;]
[šest mesecev od datuma zadnjega dejanskega ali morebitnega stika z bolno živaljo ali živaljo, okuženo s Trypanosoma equiperdum;]
[in the case of a stallion, until the animal is castrated;]
[za žrebce: dokler ni žival kastrirana;]
in the case of glanders, six months beginning on the day on which the equidae suffering from the disease or subjected with positive result to a test for the detection of the causative pathogen Burkholderia mallei or antibodies to that pathogen, were killed and destroyed;
pri smrkavosti: šest mesecev od datuma, ko so bili kopitarji, ki so zboleli za to boleznijo ali so imeli pozitiven rezultat pri testu za odkrivanje povzročitelja bolezni Burkholderia mallei ali protiteles za tega povzročitelja, usmrčeni in uničeni;
in the case of equine encephalomyelitis of any type, six months beginning on the day on which the equidae suffering from the disease have been slaughtered, except in case of West Nile virus infection where the period of six months begins on the day the infected equidae died, have been removed from the holding or fully recovered;
pri konjskem encefalomelitisu katere koli vrste: šest mesecev od datuma, ko so bili usmrčeni kopitarji, ki so zboleli za to boleznijo, razen pri okužbi z virusom zahodnega Nila, ko se šestmesečno obdobje prične na datum, ko je okuženi kopitar poginil, bil odstranjen z gospodarstva ali je popolnoma okreval;
Part II: Certification
Del II: Izdaja spričeval
in the case of infectious anaemia, until the date on which, the infected animals having been slaughtered, the remaining animals have shown a negative reaction to a Coggins test carried out on blood samples collected on two occasions three months apart;
pri infekciozni anemiji: do datuma, ko je bila reakcija živali, preostalih po usmrtitvi okuženih živali, na dva cogginsova testa, opravljena na dveh krvnih vzorcih, odvzetih v razmiku treh mesecev, negativna;
in the case of vesicular stomatitis, six months from the last case;
pri vezikularnem stomatitisu: šest mesecev od zadnjega primera;
in the case of rabies, one month from the last case;
pri steklini: en mesec od zadnjega primera;
in the case of anthrax, 15 days from the last case;]
pri antraksu: 15 dni od zadnjega primera;]
[following cases of dourine, glanders, equine encephalomyelitis of all types, equine infectious anaemia, vesicular stomatitis, anthrax or rabies all animals on the holding of species susceptible to the disease in question were slaughtered or killed and the prohibition lasted for 30 days, or 15 days in the case of anthrax, beginning on the day on which, following the destruction of the animals, the disinfection of the premises was satisfactorily completed;]
[po pojavu spolne kuge konj, smrkavosti, konjskega encefalomelitisa katere koli vrste, infekciozne anemije kopitarjev, vezikularnega stomatitisa, antraksa ali stekline so bile usmrčene vse živali na gospodarstvu iz vrst, dovzetnih za zadevno bolezen, prepoved pa je veljala 30 dni oziroma 15 dni pri antraksu, s pričetkom na datum, ko so bili po usmrtitvi živali prostori ustrezno razkuženi;]
to the best of my knowledge, it/they has/have not been in contact with equidae suffering from an infectious or contagious disease in the 15 days prior to this declaration;
kolikor mi je znano, 15 dni pred to izjavo niso bile v stiku s kopitarji, ki so zboleli za nalezljivo ali kužno boleznijo;
at the time of the inspection it/they was/were fit to be transported on the intended journey in accordance with the provisions of Regulation (EC) No 1/2005 (3).
v času pregleda so bile sposobne za načrtovani prevoz v skladu z določbami Uredbe (ES) št. 1/2005 (3).
Box I.6: shall correspond to the CITES permit number in case of equidae listed in the Washington Convention on protected species and products thereof.
Rubrika I.6: za kopitarje, navedene v Washingtonski konvenciji o zaščitenih živalskih vrstah in proizvodih iz njih, se vstavi številka dovoljenja CITES.
Box I.16: Registration number (railway wagons or container and lorries), flight number (aircraft) or name (ship).
Rubrika I.16: registrska številka (železniških vagonov ali kontejnerjev in tovornjakov), številka leta (letala) ali ime (ladje).
Box I.19: Use the appropriate Harmonised System (HS) code of the World Customs Organisation: 01.01.01 or 01.01.06.19
Rubrika I.19: uporabite ustrezno oznako harmoniziranega sistema (HS) Svetovne carinske organizacije: 01.01.01 ali 01.01.06.19.
Box I.31: Species: horse, ass, mule, hinny, zebra (including their crossings).
Rubrika I.31: vrsta: konj, osel, mula, mezeg, zebra (vključno s križanci).
Identification system: Until 31 December 2009 shall correspond to an identification number as described in Article 2 of Commission Decision 2000/68/EC, and as of 1 January 2010 to the unique life number as described in Article 2(2)(d) of and Section 1(A)(4) of Annex I to Commission Regulation (EC) No 504/2008.
Identifikacijski sistem: do 31. decembra 2009 ustreza identifikacijski številki iz člena 2 Odločbe Komisije 2000/68/ES, od 1. januarja 2010 pa enotni življenjski številki iz člena 2(d) in oddelka 1(A)(4) Priloge I k Uredbi Komisije (ES) št. 504/2008.
The information in points II.1. to II.6. is not required where there is a bilateral agreement in accordance with Article 6 of Directive 2009/156/EC.
Podatkov iz točk II.1. do II.6. se ne zahteva, če obstaja dvostranski sporazum v skladu s členom 6 Direktive 2009/156/ES.
No of the related LVU
Št. pristojne LVE:
Reagents for the enzyme-linked immunosorbent assays (ELISA) described below may be obtained from the European Community Reference Laboratory or the OIE Reference Laboratories for African horse sickness.
Reagente za spodaj opisane encimskoimunske teste (ELISA) je mogoče dobiti v referenčnem laboratoriju Evropske unije ali Referenčnih laboratorijih OIE za konjsko kugo.
COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST)
KOMPETITIVNI TEST ELISA ZA ODKRIVANJE PROTITELES PROTI VIRUSU KONJSKE KUGE (VKK) (PREDPISANI TEST)
Competitive ELISA is used to detect specific AHSV antibodies in sera from any species of equidae.
Kompetitivni test ELISA se uporablja za odkrivanje specifičnih protiteles proti VKK v serumu katere koli vrste kopitarjev.
The broad spectrum, polyclonal, immune anti-AHSV guinea-pig serum (hereinafter referred to as ‘guinea-pig antiserum’) is serogroup specific and is able to detect all known serotypes of AHS virus.
Polispecifičen, poliklonski imunski anti-VKK serum buder (v nadaljevanju: budrin antiserum) je specifičen za serološko skupino in je z njim možno prepoznati vse znane serotipe virusa konjske kuge.
The principle of the test is the interruption of the reaction between AHSV antigen and a guinea-pig antiserum by a test serum sample.
Princip testa je prekinitev reakcije med antigeni VKK in budrinim antiserumom z vzorcem preiskovanega seruma.
AHSV antibodies in the test serum sample will compete with those in the guinea-pig antiserum resulting in a reduction in the expected colour (following the addition of enzyme labelled anti-guinea-pig antibody and substrate).
Protitelesa proti VKK v preiskovanem serumu bodo tekmovala s tistimi iz budrinega antiseruma, kar bo posledično povzročilo manj intenzivno obarvanost od pričakovane (po dodatku z encimom označenih protiteles proti budrinim protitelesom in substrata).
Sera can be tested at a single dilution of 1 in 5 (spot test method) or may be titrated (serum titration method) to give dilution end-points.
Serumi se lahko testirajo bodisi pri redčenju 1:5 (metoda točkovnega testa) ali pa se titrirajo (metoda titracije seruma) kjer se določijo končne točke razredčin.
Inhibition values higher than 50 % may be regarded as positive.
Vrednosti inhibicije, ki so večje kot 50 %, se lahko obravnavajo kot pozitivne.
The test protocol described hereinafter is used in the Regional Reference Laboratory for African horse sickness in Pirbright, United Kingdom.
Spodaj opisani testni protokol se uporablja v Regionalnem referenčnem laboratoriju za konjsko kugo v Pirbrightu, Združeno kraljestvo.
Preparation of plates
Priprava plošč
Coat ELISA plates with AHSV antigen extracted from infected cell cultures and diluted in carbonate/bicarbonate buffer, pH 9,6.
V ELISA plošče nanesemo antigen VKK, ekstrahiran iz okuženih celičnih kultur in razredčen v karbonatnem/bikarbonatnem pufru, pH 9,6.
Incubate the ELISA plates overnight at 4 °C.
ELISA plošče inkubiramo čez noč pri 4 °C.
Wash plates three times by flooding and emptying the wells with phosphate buffered saline (PBS), pH 7, 2 to 7,4 pH, and blot dry on adsorbent paper.
Plošče trikrat speremo s polnjenjem in praznjenjem vdolbinic s fosfatnim pufrom (PBS), pH 7,2 do 7,4, in osušimo s pivnanjem na vpojnem papirju.
Control wells
Kontrolne vdolbinice
Titrate the positive control sera in a twofold dilution series, from 1 in 5 to 1 in 640, across column 1 in blocking buffer (PBS containing 0,05 % (v/v) Tween-20, 5,0 % (w/v) skimmed-milk powder (Cadbury’s MarvelTM) and 1 % (v/v) adult bovine serum) to give a final volume of 50 μl/well.
Pozitivne kontrolne serume titriramo v serijah dvakratnih redčenj, od 1: 5 do 1: 640, v stolpcu 1 v pufru za blokiranje (PBS z 0,05 % (volumski delež) Tween-20, 5,0 % (utežni delež) posnetega mleka v prahu (Cadbury’s MarvelTM) in 1 % (volumski delež) govejega seruma), tako da dobimo končni volumen 50 μl/vdolbinico.
Add 50 μl of the negative control serum at a dilution of 1 in 5 (10 μl serum + 40 μl blocking buffer) to wells A and B of column 2.
V vdolbinici A in B stolpca 2 dodamo 50 μl negativnega kontrolnega seruma v razredčini 1: 5 (10 μl seruma + 40 μl pufra za blokiranje).
Add 100 μl/well of blocking buffer to wells C and D of column 2 (blank).
V vdolbinici C in D stolpca 2 (slepa kontrola) dodamo po 100 μl pufra za blokiranje.
Add 50 μl of blocking buffer to wells E, F, G and H of column 2 (guinea pig control).
V vdolbinice E, F, G in H stolpca 2 (budrina kontrola) dodamo po 50 μl pufra za blokiranje.
Spot test method
Metoda točkovnega testa
Add a 1 in 5 dilution of each test serum in blocking buffer to duplicate wells of columns 3 to 12 (10 μl sera + 40 μl blocking buffer).
V po dve vdolbinici stolpcev 3 do 12 dodamo redčenje vsakega preiskovanega seruma v razmerju 1:5 v pufru za blokiranje (10 μl seruma + 40 μl pufra za blokiranje).
Serum titration method
Metoda titracije seruma
Prepare a twofold dilution series of each test sample (1 in 5 to 1 in 640) in blocking buffer across eight wells of single columns (3 to 12).
Pripravimo serije dvakratnih razredčin vsakega preiskovanega vzorca (1: 5 do 1: 640) v pufru za blokiranje preko osmih vdolbinic posameznega stolpca (3 do 12).
then
potem
Add 50 μl of guinea pig antisera, pre-diluted in blocking buffer, to all wells except the blank wells of the ELISA plate (all wells now contain a final volume of 100 μl).
V vse vdolbinice razen v vdolbinice slepe kontrole ELISA plošče dodamo 50 μl budrinega antiseruma, razredčenega v pufru za blokiranje, (vse vdolbinice sedaj vsebujejo končni volumen 100 μl).
Incubate for 1 hour at 37 °C on an orbital shaker.
Inkubiramo 1 uro pri 37 °C na rotacijskem tresalniku.
Wash plates three times and blot dry as before.
Plošče trikrat speremo in osušimo kot prej.
Add 50 μl of rabbit anti-guinea-pig horseradish peroxidase (HRP) conjugate pre-diluted in blocking buffer to each well.
V vsako vdolbinico dodamo po 50 μl kunčjega anti-budrinega konjugata, označenega s hrenovo peroksidazo (HRP), redčenega v pufru za blokiranje.
Chromogen
Kromogen
Prepare the chromogen OPD (OPD = ortho-phenyldiamine) solution according to the manufacturers’ instructions (0,4 mg/ml in sterile distilled water) just before use.
Pripravimo raztopino OPD (OPD = orto-fenildiamin) kromogena po navodilih proizvajalca (0,4 mg/ml v sterilni destilirani vodi) neposredno pred uporabo.
Add substrate (hydrogen peroxide = H2O2) to give a final concentration of 0,05 % (v/v) (1 in 2000 of a 30 % solution of H2O2).
Dodamo substrat (vodikov peroksid = H2O2), da dobimo končno koncentracijo 0,05 % (volumski delež) (redčenje 1: 2000 30 % raztopine H2O2).
Add 50 μl of the OPD solution to each well and leave plates on the bench for 10 minutes at ambient temperature.
Dodamo po 50 μl OPD raztopine v vsako vdolbinico in plošče za 10 minut pustimo na pultu pri temperaturi okolja.
Stop the reaction by the addition of 50 μl/well of 1M sulphuric acid (H2SO4).
Reakcijo prekinemo z dodatkom 50 μl 1 M žveplene kisline (H2SO4)/vdolbinico.
Reading
Odčitavanje
Read spectrophotometrically at 492 nm.
Spektofotometrično odčitavamo pri valovni dolžini 492 nm.
Expression of results
Prikaz rezultatov
Using a software package print out the optical density (OD) values, and the percentage inhibition (PI) for test and control sera based on the mean value recorded in the four guinea pig control wells.
S pomočjo programskega paketa izpišemo vrednosti absorbance (A) in procent inhibicije (PI) za preiskovane in kontrolne serume na podlagi povprečne vrednosti budrinih kontrol, zabeleženih v štirih vdolbinicah.
The data expressed as OD and PI values are used to determine whether the test has performed within acceptable limits.
Podatki, prikazani kot A in PI vrednosti, se uporabijo za ugotavljanje, ali je uspešnost testa v dovoljenih mejah.
The upper control limits (UCL) and lower control limits (LCL) for the guinea pig control are between OD values 1,4 and 0,4 respectively.
Zgornje kontrolne meje (ZKM) in spodnje kontrolne meje (SKM) za budrino kontrolo so med vrednostmi A = 1,4 oziroma A = 0,4.
The end-point titre for the positive control based on 50 % PI should be 1 in 240 (within a range from 1 in 120 to 1 in 480).
Končni titer pozitivne kontrole na temelju 50 % PI bi moral biti pri redčenju 1: 240 (v razponu od 1: 120 do 1: 480).
Any plate that fails to conform to the above criteria must be rejected.
Katera koli plošča, ki ne ustreza zgoraj navedenim kriterijem, mora biti zavržena.
However, if the positive control serum titre is greater than 1 in 480 and the test samples are still negative then the negative test samples can be accepted.
Vendar pa se lahko negativni vzorci sprejmejo, če je titer pozitivne kontrole večji kot 1: 480 in so preiskovani vzorci še vedno negativni.
The duplicate negative control serum wells and the duplicate blank wells should record PI values between + 25 % and – 25 %, and between + 95 % and + 105 %, respectively.
Dvojniki vdolbinic z negativno kontrolo seruma in dvojniki vdolbinic slepe kontrole bi morali doseči vrednost PI med + 25 % in – 25 % oziroma med + 95 % in + 105 %.
Failure to be within these limits does not invalidate the plate but does suggest that background colour is developing.
Če vrednosti niso znotraj predpisanih mej, plošča ni neveljavna, da pa slutiti, da se razvija barva ozadja.
The diagnostic threshold (cut-off value) for test sera is 50 % (PI 50 %).
Diagnostični prag (mejna vrednost) preiskovanih serumov je 50 % (PI 50 %).
Samples recording PI values greater than 50 % are recorded as positive.
Vzorci, ki imajo vrednosti PI večje od 50 %, se vrednotijo kot pozitivni.
Samples recording PI values lower than 50 % are recorded as negative.
Vzorci, ki imajo vrednosti PI manjše od 50 %, se vrednotijo kot negativni.
Samples that record PI values above and below the threshold for the duplicate wells are considered doubtful.
Vzorci z vrednostmi PI nad in pod pragom v dvojnikih vdolbinic se obravnavajo za dvomljive.
Such samples may be re-tested in the spot test and by titration.
Takšne vzorce bi bilo treba ponovno testirati s točkovnim testom in s titracijo.
Positive samples may also be titrated to provide an indication of the degree of positivity.
Pozitivne vzorce se lahko tudi titrira, da se dobi stopnjo pozitivnosti.
Spot test layout
Načrt točkovnega testa
–ve cont negative control.
kont. negativna kontrola
+ ve cont positive control.
+ kont. pozitivna kontrola
GP cont guinea pig control.
GP kont. budrina kontrola
Test sera
Preiskovani serumi
Blank
Slepa kont.
INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST)
INDIREKTNI TEST ELISA ZA ODKRIVANJE PROTITELES PROTI VIRUSU KONJSKE KUGE (VKK) (PREDPISANI TEST)
The test described hereinafter is in accordance with the test description in Chapter 2.1.11 of the OIE Manual of Standards for Diagnostic Tests and Vaccines, fourth edition, 2000.
Spodaj opisani test je v skladu z opisom testa iz Poglavja 2.1.11 OIE Priročnika standardov za diagnostične teste in cepiva, četrta izdaja, 2000.
The recombinant VP7 protein has been used as antigen for AHS virus antibody determination with a high index of sensitivity and specificity.
Rekombinantni VP7 protein se je uporabljal kot antigen za določanje protiteles proti VKK z visokim indeksom občutljivosti in specifičnosti.
Other advantages are that it is stable and not infective.
Drugi prednosti sta, da je stabilen in da ni kužen.
Solid phase
Trdna faza
ELISA plates are coated with recombinant AHSV-4 VP7 diluted in carbonate/bicarbonate buffer, pH 9,6.
V ELISA plošče nanesemo rekombinantni VKK-4 VP7, razredčen v karbonatnem/bikarbonatnem pufru, pH 9,6.
Incubate plates overnight at 4 °C.
Plošče inkubiramo čez noč pri 4 °C.
Wash the plates five times with distilled water containing 0,01 % (v/v) Tween 20 (washing solution).
Plošče petkrat speremo z destilirano vodo, ki vsebuje 0,01 % (volumski delež) Tween 20 (spiralna raztopina).
Gently tap the plates onto absorbent material to remove any residual wash.
S ploščo nežno potolčemo po vpojenem materialu, da odstranimo ostanke spiralne tekočine.
Block the plates with phosphate buffered saline (PBS) + 5 % (w/v) skimmed milk (Nestlé Dry Skim MilkTM), 200 μl/well, for 1 hour at 37 °C.
Plošče blokiramo s fosfatnim pufrom (PBS) + 5 % (utežni delež) posnetega mleka v prahu (Nestlé Dry Skim MilkTM), 200 μl/vdolbinico, za 1 uro pri 37 °C.
Remove the blocking solution and gently tap the plates onto absorbent material.
Odstranimo pufer za blokiranje in nežno potolčemo s ploščo po vpojenem materialu.
Test samples
Preiskovani vzorci
Serum samples to be tested, and positive and negative control sera, are diluted 1 in 25 in PBS + 5 % (w/v) skimmed milk + 0,05 % (v/v) Tween 20, 100 μl per well.
Serumski vzorci, pozitivni in negativni kontrolni serum se redčijo 1: 25 v PBS + 5 % (utežni delež) posnetega mleka + 0,05 % (volumski delež) Tween 20, 100 μl na vdolbinico.
Incubate for 1 hour at 37 °C.
Inkubiramo 1 uro pri 37 °C.
For titration, make a twofold dilution series from 1 in 25 (100 μl/well), one serum per plate column, and do the same with positive and negative controls.
Za titracijo pripravimo serije dvakratnih razredčin od 1: 25 (100 μl/vdolbinico), en vzorec seruma na stolpec plošče, isto naredimo s pozitivnim in negativnim kontrolnim serumom.
Wash the plates as described in step 2.1.1.2.
Plošče speremo, kot je opisano v koraku 2.1.1.2.
Dispense 100 μl/well of horseradish-peroxidase (HRP) -conjugated anti-horse gamma-globulin diluted in PBS + 5 % milk + 0,05 % Tween 20, pH 7,2.
V vdolbinice dodamo po 100 μl anti-konjskih gama globulinov, označenih s hrenovo peroksidazo (HRP), razredčenih v PBS + 5 % mleka + 0,05 % Tween 20, pH 7,2.
Cromogen/Substrate
Kromogen/substrat
Add 200 μl/well of chromogen/substrate solution (10 ml of 80,6 mM DMAB (dimethyl aminobenzaldehyde) + 10 ml of 1,56 mM MBTH (3-methyl-2-benzo-thiazoline hydrazone hydrochlorid) + 5 μl H2O2)
V vdolbinice dodamo po 200 μl raztopine kromogena/substrata (10 ml 80,6 mM DMAB (dimetil aminobezaldehid) + 10 ml 1,56 mM MBTH (3-metil-2-benzo-tiazolin hidrazon hidroklorid) + 5 μl H2O2)
Colour development is stopped by adding 50 μl of 3N H2SO4 after approximately 5 to 10 minutes (before the negative control begins to be coloured).
Razvijanje barve se ustavi z dodatkom 50 μl 3N H2SO4 po približno 5 do 10 minutah (preden se začne barvati negativna kontrola).
Other chromogens such as ABTS (2,2'-Azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]), TMB (tetramethyl benzidine), or OPD (ortho-phenyldiamine) can also be used.
Lahko se uporabijo tudi drugi kromogeni, kot so ABTS (2,2’-azino-bis-[3-etilbenzotiazolin-6-sulfonska kislina]), TMB (tetra-metil benzidin) ali OPD (orto-fenildiamin).
Read the plates at 600 nm (or 620 nm).
Plošče odčitamo pri 600 nm (ali 620 nm).
Interpretation of the results
Interpretacija rezultatov
Calculate the cut-off value by adding 0,6 to the value of the negative control (0,6 is the standard deviation derived with a group of 30 negative sera).
Izračunamo mejno vrednost, tako da vrednosti negativne kontrole dodamo 0,6 (0,6 je standardna deviacija, dobljena s skupino 30 negativnih serumov).