natgillin/translations-raw huggingface dataset:
If the flask of the distillation apparatus is not fitted with a dropping funnel, add the sodium hydroxide immediately before connecting the flask to the condenser, pouring the liquid slowly down the side.
Če bučka naprave za destilacijo ni opremljena s kapalnim lijakom, se natrijev hidroksid doda tik pred povezavo bučke s kondenzatorjem, pri čemer se tekočina vliva počasi ob steni bučke.
If the digest solidifies, recommence the determination using a larger amount of sulphuric acid (3.4) than that specified above.
Če se vsebina bučke strjuje, se določanje začne znova, vendar z večjo količino žveplove kisline (3.4), kakor je navedeno zgoraj.
For products with a low nitrogen content, the volume of sulphuric acid (3.7) to be placed in the collecting flask may be reduced, if necessary, to 10 or 15 ml and made up to 25 ml with water.
Pri proizvodih z nizko vsebnostjo dušika se lahko volumen žveplove kisline (3.7), ki se doda v zbirno bučko, po potrebi zmanjša na 10 ali 15 ml, bučka pa se dopolni do 25 ml z vodo.
For routine analysis, alternative methods of analysis can be applied for the determination of crude protein but the Kjeldahl method described in this Part C is the reference method.
Pri redni analizi se lahko za določanje surovih beljakovin uporabi nadomestna analitska metoda, vendar je Kjeldahlova metoda, opisana v tem delu C, referenčna metoda.
The equivalence of the results obtained with the alternative method (e.g. DUMAS) compared to the reference method must be demonstrated for each matrix individually.
Za vsako matrico posebej je treba dokazati, da so rezultati, pridobljeni z nadomestno metodo (npr. DUMAS), enakovredni rezultatom, pridobljenim z referenčno metodo.
As the results obtained with an alternative method, even after having verified the equivalency, might deviate slightly from the results obtained with the reference method, it is necessary to mention in the analytical report the method of analysis used for the determination of crude protein.
V poročilu o analizi je treba navesti analitsko metodo, uporabljeno za določanje surovih beljakovin, saj lahko rezultati, pridobljeni z nadomestno metodo, kljub potrditvi enakovrednosti, odstopajo od rezultatov, pridobljenih z referenčno metodo.
DETERMINATION OF UREA
DOLOČANJE SEČNINE
This method makes it possible to determine the level of urea in feed.
Ta metoda omogoča določanje ravni sečnine v krmi.
The sample is suspended in water with a clarifying agent.
Vzorec se suspendira v vodi s snovjo za zbistritev.
The suspension is filtered.
Suspenzija se filtrira.
The urea content of the filtrate is determined after the addition of 4-dimethylaminobenzaldehyde (4-DMAB) by measuring the optical density at a wavelength of 420 nm.
Doda se 4-dimetilaminobenzaldehid (4-DMAB), nato pa se določi vsebnost sečnine v filtratu z merjenjem optične gostote pri valovni dolžini 420 nm.
Solution of 4-dimethylaminobenzaldehyde: dissolve 1,6 g of 4-DMAB in 100 ml of 96 % ethanol and add 10 ml of hydrochloric acid (ρ201,19 g/ml).
Raztopina 4-dimetilaminobenzaldehida: 1,6 g 4-DMAB se raztopi v 100 ml 96-odstotnega etanola in doda 10 ml klorovodikove kisline (ρ201,19 g/ml).
This reagent keeps for a maximum period of two weeks.
Reagent se lahko hrani največ dva tedna.
Carrez solution I: dissolve in water 21,9 g of zinc acetate, Zn(CH3COO)2 2H2O and 3 g of glacial acetic acid.
Raztopina Carrez I: 21,9 g cinkovega acetata Zn(CH3COO)2 2H2O in 3 g ledoceta se raztopi v vodi.
Make up to 100 ml with water.
Dopolni se z vodo do 100 ml.
Carrez solution II: dissolve in water 10,6 g of potassium ferrocyanide, K4 Fe (CN)6 3H2O.
Raztopina Carrez II: 10,6 g kalijevega ferocianida K4 Fe (CN)6 3H2O se raztopi v vodi.
Active carbon which does not absorb urea (to be checked).
Aktivno oglje, ki ne absorbira sečnine (treba je preveriti).
Urea, 0,1 % solution (w/v).
Sečnina, 0,1-odstotna raztopina (m/v)
Mixer (tumbler): approximately 35 to 40 r.p.m.
Mešalnik: približno 35 do 40 obr./min.
Test tubes: 160 × 16 mm with ground-glass stoppers.
Epruvete: 160 × 16 mm z brušenimi zamaški
Spectrophotometer.
Spektrofotometer
Analysis of sample
Analiza vzorca
Weigh out 2 g of the sample to the nearest mg and place with 1 g of active carbon (3.4) in a 500 ml volumetric flask.
2 g vzorca se natehta na 1 mg natančno in skupaj z 1 g aktivnega oglja (3.4) prenese v 500-mililitrsko merilno bučko.
Add 400 ml of water and 5 ml of Carrez solution I (3.2), mix for approximately 30 seconds and add 5 ml of Carrez solution II (3.3).
Doda se 400 ml vode in 5 ml raztopine Carrez I (3.2), meša se približno 30 sekund in doda 5 ml raztopine Carrez II (3.3).
Mix for 30 minutes in the tumbler.
Meša se 30 minut v mešalniku.
Make up to volume with water, shake and filter.
Dopolni se z vodo do oznake, pretrese in filtrira.
Remove 5 ml of the transparent colourless filtrates, place in test tubes with ground-glass stoppers, add 5 ml of 4-DMAB solution (3.1) and mix.
5 ml prozornega brezbarvnega filtrata se odstrani in prenese v epruvete z brušenimi zamaški, doda se 5 ml raztopine 4-DMAB (3.1) ter premeša.
Place the tubes in a water bath at 20 oC (+/- 4 oC).
Epruvete se postavijo v kopel z vodo pri 20 oC (+/- 4 oC).
After 15 minutes measure the optical density of the sample solution with the spectrophotometer at 420 nm.
Po 15 minutah se izmeri optična gostota raztopine vzorca s spektrofotometrom pri 420 nm.
Compare with the blank test solution of the reagents.
Rezultat se primerja z raztopino reagentov iz slepega preskusa.
Calibration curve
Umeritvena krivulja
Remove volumes of 1, 2, 4, 5 and 10 ml of the urea solution (3.5), place in 100 ml volumetric flasks and make up the volume with water.
V 100-mililitrske merilne bučke se prenese 1, 2, 4, 5 in 10 ml raztopine sečnine (3.5), ki se dopolnijo z vodo do oznake.
Remove 5 ml from each solution, add 5 ml of 4-DMAB solution (3.1) to each of them, homogenise and measure the optical density as shown above in comparison with a control solution containing 5 ml of 4-DMAB and 5 ml of water free from urea.
Od vsake raztopine se odpipetira 5 ml, vsaki se doda 5 ml raztopine 4-DMAB (3.1), homogenizira se in izmeri optična gostota, kakor je opisano zgoraj s primerjavo s kontrolno raztopino, ki vsebuje 5 ml 4-DMAB in 5 ml vode brez sečnine.
Plot the calibration curve.
Pripravi se umeritvena krivulja.
Determine the amount of urea in the sample using the calibration curve.
Količina sečnine v vzorcu se določi iz umeritvene krivulje.
Express the result as a percentage of the sample.
Rezultat se izrazi kot masni delež vzorca.
In the case of contents of urea exceeding 3 %, reduce the sample to 1 g or dilute the original solution so that there are not more than 50 mg of urea in 500 ml.
Če vsebnost sečnine presega 3 %, se vzorec zmanjša na 1 g ali se prvotna raztopina razredči, tako da v 500 ml ni več kot 50 mg sečnine.
In the case of low contents of urea, increase the sample as long as the filtrate remains transparent and colourless.
Če je vsebnost sečnine nizka, se vzorec poveča, dokler je filtrat še prozoren in brezbarven.
If the sample contains simple nitrogenous compounds such as amino acids, the optical density shall be measured at 435 nm.
Če vzorec vsebuje preproste dušikove spojine, kot so aminokisline, se optična gostota meri pri 435 nm.
DETERMINATION OF VOLATILE NITROGENOUS BASES
DOLOČANJE HLAPNIH DUŠIKOVIH BAZ
I. BY MICRODIFFUSION
I. Z MIKRODIFUZIJO
This method makes it possible to determine the content of volatile nitrogenous bases, expressed as ammonia, in feed.
Ta metoda se uporablja za določanje vsebnosti hlapnih dušikovih baz v krmi, izraženih kot amonijak.
The sample is extracted with water and the solution clarified and filtered.
Vzorec se ekstrahira z vodo, raztopina pa zbistri in filtrira.
The volatile nitrogenous bases are displaced by microdiffusion using a solution of potassium carbonate, collected in a solution of boric acid and titrated with sulphuric acid.
Hlapne dušikove baze se ločijo z mikrodifuzijo z uporabo raztopine kalijevega karbonata, zberejo v raztopino borove kisline in titrirajo z žveplovo kislino.
Trichloroacetic acid, solution 20 % (w/v).
Trikloroocetna kislina, 20-odstotna raztopina (m/v)
Indicator: dissolve 33 mg of bromocresol green and 65 mg of methyl red in 100 ml of 95 %-96 % (v/v) of ethanol.
Indikator: 33 mg bromokrezol zelenega in 65 mg metil rdečega se raztopi v 100 ml 95–96-odstotnega (v/v) etanola.
Boric acid solution: in a 1 litre graduated flask dissolve 10 g of boric acid in 200 ml of 95 %-96 % (v/v) ethanol and 700 ml of water.
Raztopina borove kisline: v litrski merilni bučki se v 200 ml 95–96-odstotnega (v/v) etanola in 700 ml vode raztopi 10 g borove kisline.
Add 10 ml of indicator (3.2).
Doda se 10 ml indikatorja (3.2).
Mix and, if necessary, adjust the colour of the solution to light red by adding a solution of sodium hydroxide.
Premeša se, po potrebi pa se barva raztopine spremeni v svetlo rdečo z dodajanjem raztopine natrijevega hidroksida.
1 ml of this solution will fix a maximum of 300 μg of NH3.
1 ml te raztopine ustreza največ 300 μg NH3.
Saturated potassium carbonate solution: dissolve 100 g of potassium carbonate in 100 ml of boiling water.
Nasičena raztopina kalijevega karbonata: v 100 ml vrele vode se raztopi 100 g kalijevega karbonata.
Leave to cool, filter.
Pusti se, da se ohladi, ter nato filtrira.
Sulphuric acid 0,01 mol/litre.
Žveplova kislina, 0,01 mol/l
Glass or plastic Conway cells (see diagram).
Steklene ali plastične Conwayeve celice (glej diagram)
Microburettes graduated in 1/100 ml.
Mikrobirete z označenim merilom 1/100 ml
Weigh 10 g of sample to the nearest 1 mg and place with 100 ml of water in a 200 ml graduated flask.
10 g vzorca se natehta na 1 mg natančno in se s 100 ml vode prenese v 200-mililitrsko merilno bučko.
Using a pipette, introduce 1 ml of boric acid solution (3.3) into the central part of the Conway cell and 1 ml of the sample filtrate into the crown of the cell.
S pipeto se doda 1 ml raztopine borove kisline (3.3) v srednji del Conwayeve celice in 1 ml filtrata vzorca v zgornji del celice.
Cover partially with the greased lid.
Delno se pokrije z namaščenim pokrovom.
Drop 1 ml of saturated potassium carbonate solution (3.4) quickly into the crown and close the lid so that the cell is airtight.
V zgornji del celice se hitro nakapa 1 ml nasičene raztopine kalijevega karbonata (3.4), nato se neprepustno zapre.
Turn the cell carefully rotating it in a horizontal plane so that the two reagents are mixed.
Celica se pazljivo obrača v vodoravni ravnini, da se reagenta premešata.
Leave to incubate either for at least four hours at room temperature or for one hour at 40 oC.
Pusti se najmanj štiri ure pri sobni temperaturi ali eno uro pri temperaturi 40 oC.
Using a microburette (4.3), titrate the volatile bases in the boric acid solution with sulphuric acid (3.5).
Z mikrobireto (4.3) se hlapne baze v raztopini borove kisline titrirajo z žveplovo kislino (3.5).
Carry out a blank test using the same procedure but without a sample to be analysed.
Slepi preskus se izvede po istem postopku, vendar brez vzorca, ki se analizira.
1 ml of H2SO40,01 mol/litre corresponds to 0,34 mg of ammonia.
1 ml H2SO40,01 mol/l ustreza 0,34 mg amonijaka.
The difference between the results of two parallel determinations carried out on the same sample shall not exceed:
Razlika med rezultatoma dveh vzporednih določanj, izvedenih na istem vzorcu, ne presega:
10 %, in relative value, for ammonia contents of less than 1,0 %,
10 % relativne vrednosti, če je vsebnost amonijaka nižja od 1,0 %,
0,1 %, in absolute value, for ammonia contents of 1,0 % or more.
0,1 % absolutne vrednosti, če je vsebnost amonijaka 1,0 % ali več.
If the ammonia content of the sample exceeds 0,6 %, dilute the initial filtrate.
Če vsebnost amonijaka v vzorcu presega 0,6 %, se začetni filtrat razredči.
Cross-section of the lid S2 S3
Prerez celice S S1
Cross-section of the cell S S1
Tloris celice
Plan view of the cell
Tloris brušenega zamaška
Plan view of the ground-glass lid
CONWAYEVA CELICA
BY DISTILLATION
Z DESTILACIJO
This method makes it possible to determine the content of volatile nitrogenous bases, expressed as ammonia, in fish-meal containing practically no urea.
Ta metoda se uporablja za določanje vsebnosti hlapnih dušikovih baz, izraženih kot amonijak, v ribji moki, ki praktično ne vsebuje sečnine.
It is applicable only to ammonia contents of less than 0,25 %.
Uporablja se le za vsebnost amonijaka, ki je nižja od 0,25 %.
The volatile nitrogenous bases are displaced at boiling point by adding magnesium oxide and collected in a specific quantity of sulphuric acid, the excess of which is back-titrated with a solution of sodium hydroxide.
Hlapne dušikove baze se izločijo pri temperaturi vrelišča z dodatkom magnezijevega oksida in zberejo v določeno količino žveplove kisline, katere presežek se titrira z raztopino natrijevega hidroksida.
Magnesium oxide.
Magnezijev oksid
Anti-foaming emulsion (e.g. silicone).
Emulzija proti penjenju (npr. silikon)
Sulphuric acid 0,05 mol/litre.
Žveplova kislina, 0,05 mol/l
Sodium hydroxide solution 0,1 mol/litre.
Raztopina natrijevega hidroksida, 0,1 mol/l
Methyl red solution 0,3 % in 95 %-96 % (v/v) ethanol.
0,3-odstotna raztopina metil rdečega v 95–96-odstotnem (v/v) etanola
Distilling apparatus of the Kjeldahl type.
Naprava za destilacijo po Kjeldahlu
Weigh 10 g of the sample to the nearest 1 mg and place with 100 ml of water in a 200 ml graduated flask.
10 g vzorca se natehta na 1 mg natančno in s 100 ml vode prenese v 200-mililitrsko merilno bučko.
Take a quantity of clear filtrate appropriate for the presumed content of volatile nitrogenous bases (100 ml is usually suitable).
Odpipetira se volumen bistrega filtrata, ki ustreza pričakovani vsebnosti hlapnih dušikovih baz (ponavadi zadošča 100 ml).
Dilute to 200 ml and add 2 g of magnesium oxide (3.2) and a few drops of anti-foaming emulsion (3.3).
Razredči se na 200 ml, doda se 2 g magnezijevega oksida (3.2) in nekaj kapljic emulzije proti penjenju (3.3).
The solution must be alkaline to litmus paper; otherwise add some magnesium oxide (3.2).
Raztopina mora biti alkalna pri preskusu z lakmusovim papirjem, če ni, se doda magnezijev oksid (3.2).
Proceed according to 5.2 and 5.3 of the method of analysis for the determination of the crude protein content (Part C of this Annex).
Nadaljevati je treba v skladu s točkama 5.2 in 5.3 dela C te priloge, tj. Analitske metode za določanje vsebnosti surovih beljakovin.
1 ml of H2SO40,05 mol/litre corresponds to 1,7 mg of ammonia.
1 ml H2SO40,05 mol/l ustreza 1,7 mg amonijaka.
The difference between the results of two parallel determinations carried out on the same sample shall not exceed, in relative value, 10 % of ammonia.
Relativna razlika med rezultatoma dveh vzporednih določitev, izvedenih na istem vzorcu, ne presega 10 % amonijaka.
DETERMINATION OF AMINO ACIDS (EXCEPT TRYPTOPHANE)
DOLOČANJE AMINOKISLIN (RAZEN TRIPTOFANA)
This method makes the determination possible of free (synthetic and natural) and total (peptide bound and free) amino acids in feed, using an amino acid analyser.
Ta metoda se uporablja za določanje prostih (sintetičnih in naravnih) in celotnih (vezanih s peptidi in prostih) aminokislin v krmi z uporabo analizatorja aminokislin.
It is applicable to the following amino acids: cyst(e)ine, methionine, lysine, threonine, alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, isoleucine, leucine, phenylalanine, proline, serine, tyrosine and valine.
Uporablja se za naslednje aminokisline: cist(e)in, metionin, lizin, treonin, alanin, arginin, aspartat, glutaminsko kislino, glicin, histidin, izolevcin, levcin, fenilalanin, prolin, serin, tirozin in valin.
The method does not distinguish between the salts of amino acids and it cannot differentiate between D and L forms of amino acids.
S metodo ni možno ločiti soli aminokislin ter D in L oblik aminokislin.
It is not valid for the determination of tryptophan or hydroxy analogues of amino acids.
Ne uporablja se za določanje triptofana ali hidroksi-analogov aminokislin.
Co-extracted nitrogenous macromolecules are precipitated with sulfosalicylic acid and removed by filtration.
Proste aminokisline se ekstrahirajo z razredčeno klorovodikovo kislino.
The filtered solution is adjusted to pH 2,20.
Soekstrahirane dušikove makromolekule se oborijo s sulfosalicilno kislino in odstranijo s filtriranjem. pH filtrirane raztopine se uravna na 2,20.
The amino acids are separated by ion exchange chromatography and determined by reaction with ninhydrin with photometric detection at 570 nm.
Aminokisline se ločijo z ionsko izmenjevalno kromatografijo in določijo z reakcijo z ninhidrinom s fotometrično detekcijo pri 570 nm.
Total amino acids
Celotne aminokisline
The procedure chosen depends on the amino acids under investigation.
Postopek se izbere glede na preiskovane aminokisline.
Cyst(e)ine and methionine must be oxidised to cysteic acid and methionine sulphone respectively prior to hydrolysis.
Cist(e)in in metionin se morata pred hidrolizo oksidirati v cisteinsko kislino in metionin-sulfon.
Tyrosine must be determined in hydrolysates of unoxidised samples.
Tirozin se mora določiti v hidrolizatih neoksidiranih vzorcev.
All the other amino acids listed in paragraph 1 can be determined in either the oxidised or unoxidised sample.
Vse druge aminokisline, navedene v točki 1, se lahko določajo v oksidiranem ali neoksidiranem vzorcu.
Oxidation is performed at 0 oC with a performic acid/phenol mixture.
Oksidacija se izvede pri 0 oC z mešanico peroksimravljične kisline in fenola.
Excess oxidation reagent is decomposed with sodium disulphite.
Presežek oksidacijskega reagenta se razgradi z natrijevim disulfitom.
The amino acids are separated by ion exchange chromatography and determined by reaction with ninhydrin using photometric detection at 570 nm (440 nm for proline).
Aminokisline se ločijo z ionsko izmenjevalno kromatografijo in določijo z reakcijo z ninhidrinom s fotometrično detekcijo pri 570 nm (440 nm za prolin).
Double distilled water or water of equivalent quality must be used (conductivity < 10 μS).
Uporabiti je treba dvojno destilirano vodo ali vodo enakovredne kakovosti (prevodnost < 10 μS)
Hydrogen peroxide, w (w/w) = 30 %.
Vodikov peroksid, m (m/m) = 30 %
Formic acid, w (w/w) = 98 %-100 %.
Mravljična kislina, m (m/m) = 98–100 %
Phenol.
Fenol
Sodium disulphite.
Natrijev disulfit
Sodium hydroxide.
Natrijev hidroksid
5-Sulfosalicylic acid dihydrate.
5-sulfosalicilat dihidrat
Hydrochloric acid, density approximately 1,18 g/ml.
Klorovodikova kislina, približno 1,18 g/ml
tri-Sodium citrate dihydrate.
Tri-natrijev citrat dihidrat
2,2'-Thiodiethanol (thiodiglycol).
2,2'-tiodietanol (tiodiglikol)
Sodium chloride.
Natrijev klorid
Ninhydrin.
Ninhidrin
Light petroleum, boiling range 40-60 oC.
Petroleter, vrelišče 40–60 oC
Norleucine, or other compound suitable for use as internal standard.
Norlevcin ali druga spojina, primerna za uporabo kot notranji standard
Nitrogen gas (< 10 ppm oxygen).
Plinasti dušik (< 10 ppm kisika)
1-Octanol.
1-oktanol
Amino acids.
Aminokisline
Standard substances listed under paragraph 1.
Standardne snovi iz točke 1.
Pure compounds containing no water of crystallisation.
Čiste spojine, ki ne vsebujejo kristalne vode.
Dry under vacuum over P2O5 or H2SO4 for 1 week prior to use.
Pred uporabo se suši 1 teden pod vakuumom s P2O5 ali H2SO4.
Cysteic acid.
Cisteinska kislina
Methionine sulphone.
Metionin-sulfon
Sodium hydroxide solution, c = 7,5 mol/l:
Raztopina natrijevega hidroksida, c = 7,5 mol/l:
Dissolve 300 g NaOH (3.5) in water and make up to 1 litre.
300 g NaOH (3.5) se raztopi v vodi in dopolni z vodo do 1 litra.
Sodium hydroxide solution, c = 1 mol/l:
Raztopina natrijevega hidroksida, c = 1 mol/l:
Dissolve 40 g NaOH (3.5) in water and make up to 1 litre.
40 g NaOH (3.5) se raztopi v vodi in dopolni z vodo do 1 litra.
Formic acid — phenol solution:
Mravljična kislina – raztopina fenola:
Mix 889 g formic acid (3.2) with 111 g water and add 4,73 g phenol (3.3).
889 g mravljične kisline (3.2) se zmeša s 111 g vode ter se doda 4,73 g fenola (3.3).
Hydrolysis mixture, c = 6 mol HCl/l containing 1 g phenol/l:
Hidrolizna mešanica, c = 6 mol HCl/l, ki vsebuje 1 g fenola/l:
Add 1 g phenol (3.3) to 492 ml HCl (3.7) and make up to 1 litre with water.
1 g fenola (3.3) se doda k 492 ml HCl (3.7) in dopolni z vodo do 1 litra.
Extraction mixture, c = 0,1 mol HCl/l containing 2 % thiodiglycol: Take 8,2 ml HCl (3.7), dilute with approximately 900 ml water, add 20 ml thiodiglycol (3.9) and make up to 1 litre with water, (do not mix 3.7 and 3.9 directly).
Mešanica ekstrakcije, c = 0,1 mol HCl/l, ki vsebuje 2 % tiodiglikola: 8,2 ml HCl (3.7) se razredči s približno 900 ml vode, doda se 20 ml tiodiglikola (3.9) in dopolni z vodo do 1 litra (3.7 in 3.9 se ne smeta zmešati neposredno).
5-Sulfosalicylic acid, ß = 6 %:
5-sulfosalicilna kislina, ß = 6 %:
Dissolve 60 g 5-sulfosalicylic acid (3.6) in water and make up to 1 l with water.
60 g 5-sulfosalicilne kisline (3.6) se raztopi v vodi in dopolni z vodo do 1 litra.
Oxidation mixture (Performic acid — phenol):
Oksidacijska mešanica (peroksimravljična kislina – fenol):
Mix 0,5 ml hydrogen peroxide (3.1) with 4,5 ml formic acid-phenol solution (3.19) in a small beaker.
0,5 ml vodikovega peroksida (3.1) se zmeša s 4,5 ml raztopine mravljične kisline in fenola (3.19) v majhni čaši.
Incubate at 20-30 oC for 1 hour in order to form performic acid, then cool on an ice-water bath (15 min.) before adding to the sample.
Inkubira se 1 uro pri 20–30 oC, da nastane peroksimravljična kislina, nato se ohladi v ledeni kopeli (15 min), preden se doda vzorcu.
Caution: Avoid contact with skin and wear protective clothing.
Opozorilo: Izogibati se je treba stiku s kožo in nositi je treba zaščitna oblačila.
Citrate buffer, c = 0,2 mol Na+/l, pH 2,20:
Citratni pufer, c = 0,2 mol Na+/l, pH 2,20:
Make up to 1 litre with water.
Dopolni se z vodo do 1 litra.
Elution buffers, prepared according to conditions for the analyser used (4.9).
Pufri za eluiranje, pripravljeni v skladu z navodili za uporabljeni analizator (4.9)
Ninhydrin reagent, prepared according to conditions for the analyser used (4.9).
Reagent ninhidrin, pripravljen v skladu z navodili za uporabljeni analizator (4.9)
Standard solutions of amino acids.
Standardne raztopine aminokislin.
These solutions shall be stored below 5 oC.
Te raztopine se hranijo pri temperaturi do 5 oC.
Stock standard solution of amino acids (3.16.1).
Osnovna standardna raztopina aminokislin (3.16.1).
c = 2,5 μmol/ml of each in hydrochloric acid.
c = 2,5 μmol/ml za vsako v klorovodikovi kislini.
May be obtained commercially.
Lahko se priskrbi iz proste prodaje.
Stock standard solution of cysteic acid and methionine sulphone, c = 1,25 μmol/ml.
Osnovna standardna raztopina cisteinske kisline in metionin-sulfona, c = 1,25 μmol/ml.
Dissolve 0,2115 g cysteic acid (3.16.2) and 0,2265 g methionine sulphone (3.16.3) in citrate buffer (3.24) in a 1 litre graduated flask and make up to mark with citrate buffer.
V litrski merilni bučki se raztopi 0,2115 g cisteinske kisline (3.16.2) in 0,2265 g metionin-sulfona (3.16.3) v citratnem pufru (3.24) ter dopolni s citratnim pufrom do oznake.
Store below 5 oC for not more than 12 months.
Hrani se največ 12 mesecev pri temperaturi do 5 oC.
This solution is not used if the stock standard solution (3.27.1) contains cysteic acid and methionine sulphone.
Ta raztopina se ne uporablja, če osnovna standardna raztopina (3.27.1) vsebuje cisteinsko kislino in metionin-sulfon.
Stock standard solution of the internal standard e.g. norleucine, c = 20 μmol/ml.
Osnovna standardna raztopina notranjega standarda, npr. norlevcina, c = 20 μmol/ml.
Dissolve 0,6560 g norleucine (3.13) in citrate buffer (3.24) in a graduated flask and make up to 250 ml with citrate buffer.
V merilni bučki se raztopi 0,6560 g norlevcina (3.13) v citratnem pufru (3.24) in dopolni s citratnim pufrom do 250 ml.
Store below 5 oC for no more than 6 months.
Hrani se največ 6 mesecev pri temperaturi do 5 oC.