natgillin/translations-raw huggingface dataset:
Slurry 100 g resin with 500 ml hydrochloric acid solution (3.5) and heat on a hot plate to boiling, stirring continuously.
100 g smole se suspendira v 500 ml raztopine klorovodikove kisline (3.5) in se med neprestanim mešanjem segreva na vroči plošči do vretja.
Allow to cool and decant off the acid.
Ohladi se in kislina se oddekantira.
Filter through a filter paper under vacuum.
Filtrira se skozi papirni filter pod vakuumom.
Wash the resin twice with 500 ml portions of water and then with 250 ml of methanol (3.2).
Smola se dvakrat spere s po 500 ml vode, nato pa še z 250 ml metanola (3.2).
Rinse the resin with a further 250 ml portion of methanol and dry by passing air through the filter cake.
Nato se še enkrat spere s 250 ml metanola in osuši z zrakom, ki prehaja skozi filtrirano pogačo.
Store the dried resin in a stoppered bottle.
Suha smola se hrani v zaprti steklenici.
Standard substance: pure methyl benzoquate (7-benzyloxy-6-butyl-3-methoxycarbonyl-4-quinolone)
Standardna snov: čisti metil benzokvat (7-benziloksi-6-butil-3-metoksikarbonil-4-kinolon)
Methyl benzoquate stock standard solution, 500 μg/ml
Standardna osnovna raztopina metil benzokvata, 500 μg/ml
Weigh to the nearest 0,1 mg, 50 mg of standard substance (3.7), dissolve in methanesulfonic acid solution (3.4) in a 100 ml graduated flask, make up to the mark and mix.
50 mg standardne snovi (3.7) se natehta na 0,1 mg natančno in raztopi v raztopini metansulfonske kisline (3.4) v 100-mililitrski merilni bučki, dopolni se do oznake in premeša.
Methyl benzoquate intermediate standard solution, 50 μg/ml
Vmesna standardna raztopina metil benzokvata, 50 μg/ml
Transfer 5,0 ml of methyl benzoquate stock standard solution (3.7.1) into a 50 ml graduated flask, make up to the mark with methanol (3.2) and mix.
5,0 ml osnovne standardne raztopine metil benzokvata (3.7.1) se prenese v 50-mililitrsko merilno bučko, dopolni do oznake z metanolom (3.2) in premeša.
Transfer 1,0, 2,0, 3,0, 4,0 and 5,0 ml of methyl benzoquate intermediate standard solution (3.7.2) into a series of 25 ml graduated flasks.
V serijo 25-mililitrskih merilnih bučk se prenese 1,0, 2,0, 3,0, 4,0 in 5,0 ml vmesne standardne raztopine metil benzokvata (3.7.2).
Make up to the mark with the mobile phase (3.3) and mix.
Dopolni se do oznake z mobilno fazo (3.3) in premeša.
These solutions have concentrations of 2,0, 4,0, 6,0, 8,0 and 10,0 μg/ml methyl benzoquate respectively.
Koncentracija metil benzokvata v teh raztopinah je 2,0, 4,0, 6,0, 8,0 in 10,0 μg/ml.
Laboratory shaker
Laboratorijski stresalnik
Glass column (250 mm × 15 mm) fitted with a stopcock and reservoir of approximately 200 ml capacity
Steklena kolona (250 mm × 15 mm) s petelinčkom in kapaciteto rezervoarja približno 200 ml
Liquid chromatographic column: 300 mm × 4 mm, C18, 10 μm packing or equivalent
Kolona za tekočinsko kromatografijo: 300 mm × 4 mm, C18, polnitev 10 μm ali enakovredna
A blank feed shall be analysed to check that neither methyl benzoquate nor interfering substances are present.
Z analizo slepe krme se zagotovi, da niso prisotni niti metil benzokvat niti moteče snovi.
A recovery test shall be carried out by analysing the blank feed which has been fortified by addition of a quantity of methyl benzoquate, similar to that present in the sample.
Preskus izkoristka se izvede z analizo slepe krme, ki se ji doda količina metil benzokvata, podobna količini v vzorcu.
To fortify at a level of 15 mg/kg, add 600 μl of the stock standard solution (3.7.1) to 20 g of the blank feed, mix and wait for 10 minutes before proceeding with the extraction step (5.2).
Za koncentracijo 15 mg/kg se k 20 g slepe krme doda 600 μl osnovne standardne raztopine (3.7.1), premeša se in počaka 10 minut, preden se začne postopek ekstrakcije (5.2).
Note for the purpose of this method, the blank feed shall be similar in type to that of the sample and on analysis methyl benzoquate must not be detected.
Opomba: Pri tej metodi je vrsta slepe krme podobna vrsti vzorca in pri analizi se dokaže odsotnost metil benzikvata.
Weigh to the nearest 0,01 g, approximately 20 g of the prepared sample and transfer to a 250 ml conical flask.
Približno 20 g pripravljenega vzorca se natehta na 0,01 g natančno in prenese v 250-mililitrsko erlenmajerico.
Liquid-liquid partition
Ločevanje tekočina-tekočina
Transfer into a 500 ml separating funnel containing 100 ml of hydrochloric acid solution (3.5), 25,0 ml of the filtrate obtained in (5.2).
V 500-mililitrski lij ločnik, v katerem je 100 ml raztopine klorovodikove kisline (3.5), se prenese 25,0 ml filtrata, ki nastane pri postopku iz točke 5.2.
Add 100 ml dichloromethane (3.1) to the funnel and shake for one minute.
V lij se doda 100 ml diklorometana (3.1) in stresa eno minuto.
Allow the layers to separate and run off the lower (dichloromethane) layer into a 500 ml round-bottomed flask.
Počaka se, da se plasti ločijo, spodnja (diklorometanska) plast pa se odlije v 500-mililitrsko bučko z okroglim dnom.
Repeat the extraction of the aqueous phase with two further 40-ml portions of dichloromethane and combine these with the first extract in the round-bottomed flask.
Ekstrakcija vodne faze se dvakrat ponovi s po 40 ml diklorometana, ki se priključijo prvemu ekstraktu v bučki z okroglim dnom.
Evaporate the dichloromethane extract down to dryness on the rotary evaporator (4.2) operating under reduced pressure at 40 oC.
Ekstrakt diklorometana se upari do suhega v rotacijskem uparjalniku (4.2) pod znižanim tlakom ter pri 40 oC.
Dissolve the residue in 20 to 25 ml methanol (3.2), stopper the flask and retain the whole of the extract for ion-exchange chromatography (5.4).
Ostanek se raztopi v 20–25 ml metanola (3.2), bučka se zamaši, celotni ekstrakt pa se shrani za ionsko izmenjevalno kromatografijo (5.4).
Ion-exchange chromatography
Ionska izmenjevalna kromatografija
Preparation of the cation-exchange column
Priprava kationske izmenjevalne kolone
Insert a plug of glass wool into the lower end of a glass column (4.3).
V spodnji del steklene kolone (4.3) se vstavi zamašek iz steklene volne.
Prepare a slurry of 5,0 g of the treated cation-exchange resin (3.6) with 50 ml of hydrochloric acid (3.5), pour into the glass column and allow to settle.
Pripravi se suspenzija s 5,0 g obdelane kationske izmenjevalne smole (3.6) in 50 ml klorovodikove kisline (3.5), nato pa se vlije v stekleno kolono in počaka, da se usede.
Run out the excess acid to just above the resin surface and wash the column with water until the effluent is neutral to litmus.
Presežek kisline se odstrani do višine tik nad površino smole, nato pa se kolona spira z vodo do nevtralne reakcije eluata na lakmusov papir.
Transfer 50 ml methanol (3.2) onto the column and allow to drain down to the resin surface.
50 ml metanola (3.2) se prenese v kolono in pusti, da prodre na površino smole.
By means of a pipette, carefully transfer the extract obtained in (5.3) onto the column.
Nastali ekstrakt (5.3) se pazljivo odpipetira na kolono.
Rinse the round-bottomed flask with two portions of 5 to 10 ml methanol (3.2) and transfer these washings to the column.
Bučka z okroglim dnom se dvakrat spere s po 5–10 ml metanola (3.2), izpirka pa se preneseta na kolono.
Run the extract down to the resin surface and wash the column with 50 ml methanol, ensuring that the flow rate does not exceed 5 ml per minute.
Ekstrakt se spusti do površine smole, kolona pa se spere s 50 ml metanola, pri čemer hitrost pretoka ne sme preseči 5 ml na minuto.
Discard the effluent.
Eluat se zavrže.
Elute the methyl benzoquate from the column using 150 ml of methanesulfonic acid solution (3.4) and collect the column eluate in a 250 ml conical flask.
Metil benzokvat se iz kolone eluira s 150 ml raztopine metansulfonske kisline (3.4), eluat s kolone pa se zbere v 250-mililitrsko erlenmajerico.
Add 300 ml of hydrochloric acid solution (3.5) and 130 ml of dichloromethane (3.1).
Doda se 300 ml raztopine klorovodikove kisline (3.5) in 130 ml diklorometana (3.1).
Shake for 1 minute and allow the phases to separate.
Stresa se 1 minuto in pusti, da se fazi ločita.
Run off the lower (dichloromethane) layer into a 500 ml round bottomed flask.
Spodnja (diklorometanska) plast se odtoči v 500-mililitrsko bučko z okroglim dnom.
Repeat the extraction of the aqueous phase with two further 70 ml portions of dichloromethane and combine these extracts with the first in the round-bottomed flask.
Ekstrakcija vodne faze se dvakrat ponovi s po 70 ml diklorometana, oba ekstrakta pa se združita s prvim v bučki z okroglim dnom.
Dissolve the residue in the flask with approximately 5 ml of methanol (3.2) and transfer this solution quantitatively to a 10 ml graduated flask.
Ostanek v bučki se raztopi v približno 5 ml metanola (3.2), raztopina pa se prenese v 10-mililitrsko merilno bučko.
Rinse the round-bottomed flask with a further two portions of 1 to 2 ml of methanol and transfer these to the graduated flask.
Bučka z okroglim dnom se dvakrat spere s po 1–2 ml metanola, izpirka pa se preneseta v merilno bučko.
Make up to the mark with methanol and mix.
Dopolni se z metanolom do oznake in premeša.
An aliquot portion is filtered through a membrane filter (4.6).
Alikvot se filtrira skozi membranski filter (4.6).
Reserve this solution for HPLC-determination (5.6).
Ta raztopina se shrani za določanje s HPLC (5.6).
The following conditions are offered for guidance, other conditions may be used provided that they give equivalent results:
Naslednji pogoji se lahko uporabljajo kot smernica, lahko pa se uporabljajo drugi pogoji, če zagotavljajo enakovredne rezultate:
liquid chromatographic column (4.4.1),
kolona za tekočinsko kromatografijo (4.4.1),
HPLC mobile phase: methanol-water mixture (3.3),
HPLC-mobilna faza: mešanica metanola in vode (3.3),
flow rate: 1 to 1,5 ml/minute,
hitrost pretoka: 1–1,5 ml/min,
detection wavelength: 265 nm,
valovna dolžina detekcije: 265 nm,
Check the stability of the chromatographic system, injecting the calibration solution (3.7.3) containing 4 μg/ml several times, until constant peak heights or areas and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizganjem raztopine za umerjanje (3.7.3) s koncentracijo 4 μg/ml, dokler se ne dosežejo konstantne višine (ali površine) vrhov in konstantni retencijski časi.
Inject each calibration solution (3.7.3) several times and measure the peak heights (areas) for each concentration.
Vsaka raztopina za umerjanje (3.7.3) se večkrat vbrizga in za vsako koncentracijo se izmerijo višine (površine) vrhov.
Inject the sample extract (5.5) several times, using the same volume as taken for the calibration solutions and determine the mean peak height (area) of the methyl benzoquate peaks.
Ekstrakt vzorca (5.5) se večkrat vbrizga, pri tem se uporabijo enaki volumni kakor pri raztopini za umerjanje, ter določijo srednje vrednosti višin (površin) vrhov metil benzokvata.
Determine the concentration of the sample solution in μg/ml from the mean height (area) of the methyl benzoquate peaks of the sample solution by reference to the calibration graph (5.6.2).
Koncentracija raztopine vzorca v μg/ml se določi iz srednjih vrednosti vrhov (površin) metil benzokvata iz umeritvene krivulje (5.6.2).
The content of methyl benzoquate w (mg/kg) of the sample is given by the following formula:
Vsebnost metil benzokvata (w) (mg/kg) v vzorcu se izračuna po naslednji formuli:
c methyl benzoquate concentration of the sample solution in μg/ml,
c koncentracija metil benzokvata v raztopini vzorca v μg/ml;
The identity of the analyte can be confirmed by co-chromatography, or by using a diode-array detector by which the spectra of the sample extract and the calibration solution (3.7.3) containing 10 μg/ml are compared.
Identiteta analita se lahko potrdi z dodatno kromatografijo ali z detektorjem s serijo diod, s katerim se primerjata spekter ekstrakta vzorca in spekter raztopine za umerjanje (3.7.3) s koncentracijo 10 μg/ml.
A sample extract is fortified by addition of an appropriate amount of the intermediate standard solution (3.7.2).
Ekstraktu vzorca se doda ustrezna količina vmesne standardne raztopine (3.7.2).
The amount of added methyl benzoquate must be similar to the estimated amount of methyl benzoquate in the sample extract.
Količina dodanega metil benzokvata mora biti podobna ocenjeni količini metil benzokvata v ekstraktu vzorca.
Only the height of the methyl-benzoquate peak shall be enhanced after taking into account both the amount added and the dilution of the extract.
Povišan je le vrh metil benzokvata ob upoštevanju dodane količine in razredčitve ekstrakta.
the wavelength of maximum absorption of the sample and of the standard spectra recorded at the peak apex on the chromatogram must be the same within a margin determined by the resolving power of the detection system.
valovni dolžini maksimalne absorpcije spektra vzorca in spektra standarda, zabeleženi na najvišji točki vrha kromatograma, morata biti enaki znotraj meja, določenih z ločljivostjo sistema za detekcijo.
between 220 and 350 nm, the sample and standard spectra recorded at the peak apex on the chromatogram must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
med 220 in 350 nm se spekter vzorca in spekter standarda na najvišji točki kromatografskega vrha za navedene dele spektra ne smeta razlikovati znotraj območja od 10 do 100 % relativne absorbance.
between 220 and 350 nm, the spectra of the upslope, apex and downslope of the peak produced by the sample extract must not be different from each other for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
med 220 in 350 nm se spektri naraščanja, najvišje točke vrha in padanja vrha, pridobljeni iz ekstrakta vzorca, za navedene dele spektra ne smejo razlikovati med seboj v območju 10 do 100 % relativne absorbance.
This criterion is met when the same maxima are present and when at no observed points the deviation between the spectra does not exceed 15 % of the absorbance of the spectrum of the apex.
To merilo je izpolnjeno, ko so prisotne iste najvišje točke in ko odmik med spektri na nobeni od opazovanih točk ne preseže 15 % absorbance spektra najvišje točke.
The difference between the results of two parallel determinations carried out on the same sample must not exceed: 10 % relative to the higher result for methyl benzoquate contents between 4 and 20 mg/kg.
Razlika med rezultatoma dveh vzporednih določanj, izvedenih na istem vzorcu, ne sme presegati 10 % višjega rezultata za vsebnosti metil benzokvata 4–20 mg/kg.
For a fortified blank sample the recovery shall be at least 90 %.
Za slepi vzorec z dodatkom je izkoristek najmanj 90-odstoten.
Five samples were analysed by 10 laboratories.
10 laboratorijev je analiziralo 5 vzorcev.
Blank
Slepi preskus
Meal 1
Obrok 1
Pellet 1
Briketi 1
Meal 2
Obrok 2
Pellet2
Briketi 2
ND Not detected
ND ni bilo ugotovljeno;
DETERMINATION OF OLAQUINDOX
DOLOČANJE OLAKVINDOKSA
2-[N-2'-(hydroxyethyl)carbamoyl]-3-methylquinoxaline-N1,N4-dioxide
2-[N-2'-(hidroksietil)karbamoil]-3-metilkvinoksalin-N1,N4-dioksid
This method makes it possible to determine the level olaquindox in feed.
Ta metoda omogoča določanje ravni olakvindoksa v krmi.
The sample is extracted by a water-methanol mixture.
Vzorec se ekstrahira z mešanico vode in metanola.
The content of olaquindox is determined by reversed-phase high-performance liquid chromatography (HPLC) using an UV detector.
Vsebnost olakvindoksa se določi s tekočinsko kromatografijo visoke ločljivosti (HPLC) z reverzno fazo z uporabo UV-detektorja.
Mobile phase for HPLC.
Mobilna faza za HPLC
Water (3.3)-methanol (3.2) mixture, 900 +100 (V + V).
Mešanica vode (3.3) in metanola (3.2), 900 + 100 (V + V)
Standard substance: pure olaquindox 2-[N-2'-(hydroxyethyl)carbamoyl]-3-methylquinoxaline-N1,N4-dioxide, E 851.
Standardna snov: čisti olakvindoks 2-[N-2'-(hidroksietil)karbamoil]-3-metilkvinoksalin-N1,N4-dioksid, E 851
Olaquindox stock standard solution, 250 μg/ml
Osnovna standardna raztopina olakvindoksa, 250 μg/ml
Weigh to the nearest 0,1 mg 50 mg of olaquindox (3.5) in a 200 ml graduated flask and add ca. 190 ml water.
50 mg olakvindoksa (3.5) se natehta na 0,1 mg natančno in prenese v 200-mililitrsko merilno bučko, nato pa se doda približno 190 ml vode.
Then place the flask for 20 min. into an ultrasonic bath (4.1).
Bučka se nato postavi za 20 minut v ultrazvočno kopel (4.1).
After ultrasonic treatment bring the solution to room temperature, make up to the mark with water and mix.
Po ultrazvočni obdelavi se raztopina segreje na sobno temperaturo, dopolni se z vodo do oznake in premeša.
Wrap the flask with aluminium foil and store in a refrigerator.
Bučka se ovije v aluminijasto folijo in shrani v hladilniku.
This solution must be prepared fresh each month.
To raztopino je treba pripraviti svežo vsak mesec.
Olaquindox intermediate standard solution, 25 μg/ml
Vmesna standardna raztopina olakvindoksa, 25 μg/ml
Transfer 10,0 ml of the stock standard solution (3.5.1) into a 100 ml graduated flask, make up to the mark with the mobile phase (3.4) and mix.
10,0 ml osnovne standardne raztopine (3.5.1) se prenese v 100-mililitrsko merilno bučko, dopolni z mobilno fazo (3.4) do oznake in premeša.
This solution must be prepared fresh each day.
To raztopino je treba pripraviti svežo vsak dan.
Into a series of 50 ml graduated flasks transfer 1,0, 2,0, 5,0, 10,0, 15,0 and 20,0 ml of the intermediate standard solution (3.5.2).
V serijo 50-mililitrskih merilnih bučk se prenese 1,0, 2,0, 5,0, 10,0, 15,0 in 20,0 ml vmesne standardne raztopine (3.5.2).
Make up to the mark with the mobile phase (3.4) and mix.
Dopolni se z mobilno fazo (3.4) do oznake in premeša.
Wrap the flasks with aluminium foil.
Bučke se ovijejo v aluminijasto folijo.
These solutions correspond to 0,5, 1,0, 2,5, 5,0, 7,5 and 10,0 μg of olaquindox per ml respectively.
Te raztopine ustrezajo 0,5, 1,0, 2,5, 5,0, 7,5 in 10,0 μg olakvindoksa na ml.
These solutions must be prepared fresh each day.
Te raztopine je treba pripraviti sveže vsak dan.
HPLC equipment with variable wavelength ultraviolet detector or diode array detector
Oprema za HPLC z detektorjem ultravijolične svetlobe z nastavljivo valovno dolžino ali detektorjem s serijo diod
Liquid chromatographic column, 250 mm × 4 mm, C18, 10 μm packing, or equivalent
Kolona za tekočinsko kromatografijo, 250 mm × 4 mm C18, 10 μm polnitev ali enakovredna
Olaquindox is light sensitive.
Olakvindoks je občutljiv na svetlobo.
Carry out all procedures under subdued light or use amber glassware.
Vsi postopki se izvajajo v temnem prostoru ali pa se uporabijo posode iz rjavega stekla.
A blank feed shall be analysed to check that neither olaquindox nor interfering substances are present.
Z analizo slepe krme se zagotovi, da niso prisotni niti olakvindoks niti moteče snovi.
A recovery test shall be carried out by analysing the blank feed which has been fortified by addition of a quantity of olaquindox, similar to that present in the sample.
Preskus izkoristka se izvede z analizo slepe krme, ki se ji doda količina olakvindoksa, podobna količini v vzorcu.
To fortify at a level of 50 mg/kg, transfer 10,0 ml of the stock standard solution (3.5.1) to a 250 ml conical flask and evaporate the solution to ca. 0,5 ml.
Za koncentracijo 50 mg/kg se 10,0 ml osnovne standardne raztopine (3.5.1) prenese v 250-mililitrsko erlenmajerico, raztopina pa se upari do približno 0,5 ml.
Add 50 g of the blank feed, mix thoroughly and leave for 10 min. mixing again several times before proceeding with the extraction step (5.2).
Doda se 50 g slepe krme, dobro se premeša in pusti 10 minut ter vmes nekajkrat ponovno premeša, nato pa se nadaljuje s postopkom ekstrakcije (5.2).
For the purpose of this method the blank feed shall be similar in type to that of the sample and olaquindox must not be detected.
Pri tej metodi je vrsta slepe krme podobna tisti iz vzorca in pri analizi se dokaže odsotnost olakvindoksa.
Weigh to the nearest 0,01 g, approximately 50 g of the sample.
Približno 50 g vzorca se natehta na 0,01 g natančno.
Transfer to a 1000 ml conical flask, add 100 ml of methanol (3.1) and place the flask for 5 min. in the ultrasonic bath (4.1).
Prenese se v 1000-mililitrsko erlenmajerico, doda se 100 ml metanola (3.1), erlenmajerica pa se postavi za 5 minut v ultrazvočno kopel (4.1).
Add 410 ml water and leave in the ultrasonic bath for further 15 min. Remove the flask from the ultrasonic bath, shake it for 30 min. on the shaker (4.2) and filter through a folded filter.
Doda se 410 ml vode in se pusti še 15 minut v ultrazvočni kopeli. Bučka se odstrani iz ultrazvočne kopeli, 30 minut se stresa s stresalnikom (4.2), nato pa se filtrira skozi naguban filter.
Transfer 10,0 ml of the filtrate into a 20 ml graduated flask, make up to the mark with water and mix.
10,0 ml filtrata se prenese v 20-mililitrsko merilno bučko, dopolni se z vodo do oznake in premeša.
Analytical column (4.3.1)
Analitska kolona (4.3.1)
Mobile Phase (3.4):
Mobilna faza (3.4):
water (3.3)-methanol (3.2) mixture, 900 + 100 (V + V)
mešanica vode (3.3) in metanola (3.2), 900 + 100 (V + V)
Check the stability of the chromatographic system, injecting several times the calibration solution (3.5.3) containing 2,5 μg/ml, until constant peak heights and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizgavanjem raztopine za umerjanje (3.5.3) s koncentracijo 2,5 μg/ml, dokler se ne dosežejo konstantne višine vrhov in konstantni retencijski časi.
Inject each calibration solution (3.5.3) several times and determine the mean peak heights (areas) for each concentration.
Za vsako koncentracijo se vsaka raztopina za umerjanje (3.5.3) večkrat vbrizga, nato pa se določijo se srednje vrednosti višin (površin) vrhov.
Plot a calibration graph using the mean peak heights (areas) of the calibration solutions as the ordinates and the corresponding concentrations in μg/ml as the abscissae.
Umeritvena krivulja se pripravi tako, da se na ordinato nanesejo srednje vrednosti višin (površin) vrhov raztopin za umerjanje, na absciso pa ustrezne koncentracije v μg/ml.
Inject the sample extract (5.2) several times using the same volume as taken for the calibration solutions and determine the mean peak height (area) of the olaquindox peaks.
Ekstrakt vzorca (5.2) se vbrizga večkrat, pri čemer se uporabijo enaki volumni kakor pri raztopini za umerjanje, nato pa se določijo srednje vrednosti višin (površin) vrhov olakvindoksa.
From the mean height (area) of the olaquindox peaks of the sample solution determine the concentration of the sample solution in μg/ml by reference to the calibration graph (5.3.2).
Koncentracija raztopine vzorca v μg/ml se določi iz srednje vrednosti višin (površin) vrhov olakvindoksa iz umeritvene krivulje (5.3.2).
The olaquindox content w in mg/kg of the sample is given by the following formula:
Vsebnost olakvindoksa v vzorcu (w) v mg/kg se izračuna po naslednji formuli:
c olaquindox concentration of the sample extract (5.2) in μg/ml
c koncentracija olakvindoksa v ekstraktu vzorca (5.2) v μg/ml;
m weight of the test portion in g (5.2).
m masa preskusnega vzorca v g (5.2).
The identity of the analyte can be confirmed by co-chromatography, or by using a diode-array detector by which the spectra of the sample extract (5.2) and the calibration solution (3.5.3) containing 5,0 μg/ml are compared.
Identiteta analita se lahko potrdi z dodatno kromatografijo ali z detektorjem s serijo diod, s katerim se primerjata spekter ekstrakta vzorca (5.2) in spekter raztopine za umerjanje (3.5.3) s koncentracijo 5,0 μg/ml.
A sample extract (5.2) is fortified by addition of an appropriate amount of calibration solution (3.5.3).
Ekstraktu vzorca (5.2) se doda ustrezna količina raztopine za umerjanje (3.5.3).
The amount of added olaquindox must be similar to the amount of olaquindox found in the sample extract.
Količina dodanega olakvindoksa mora biti podobna količini olakvindoksa v ekstraktu vzorca.
Only the height of the olaquindox peak shall be enhanced after taking into account both the amount added and the dilution of the extract.
Povišan je le vrh olakvindoksa ob upoštevanju dodane količine in razredčitve ekstrakta.