natgillin/translations-raw huggingface dataset:
The calibration solution ‘0’ must not be used as the blank.
Raztopina za umerjanje „0“ se ne sme uporabljati kot slepa raztopina.
Measurement of the atomic absorption
Merjenje atomske absorpcije
Measure the atomic absorption of the calibration solutions and of the solution to be analysed using an oxidising air-acetylene flame at the following wavelengths:
Atomska absorpcija raztopin za umerjanje in raztopine, ki se analizira, se izmeri z uporabo oksidnega plamena zrak-acetilen pri naslednjih valovnih dolžinah:
Carry out each measurement four times.
Vsako merjenje se izvede štirikrat.
Mineral feed
Mineralna krma
If the sample contains no organic matter, prior ashing is unnecessary.
Če vzorec ne vsebuje organskih snovi, predhodno upepeljevanje ni potrebno.
Proceed as described in point 5.1.1.1 starting from the second paragraph.
Nadaljuje se v skladu z drugim odstavkom točke 5.1.1.1.
Evaporation with hydrofluoric acid may be omitted.
Uparevanje s klorovodikovo kislino se lahko izpusti.
Using a calibration curve, calculate the trace element concentration in the solution to be analysed and express the result in milligrams of trace element per kilogram of sample (ppm).
Koncentracija elementa v sledovih v raztopini, ki se analizira, se izračuna s pomočjo umeritvene krivulje, rezultat pa se izrazi v miligramih elementa v sledovih na kilogram vzorca (ppm).
5 mg/kg, in absolute value, for contents of the trace element concerned up to 50 mg/kg,
5 mg/kg absolutne vrednosti za vsebnosti zadevnega elementa v sledovih do 50 mg/kg,
10 % of the higher result for contents of the trace element concerned from 50 and up to 100 mg/kg,
10 % višjega rezultata za vsebnosti zadevnega elementa v sledovih 50–100 mg/kg,
10 mg/kg, in absolute value, for contents of the trace element concerned from 100 and up to 200 mg/kg,
10 mg/kg absolutne vrednosti za vsebnosti zadevnega elementa v sledovih 100–200 mg/kg,
5 % of the higher result for contents of the trace element concerned above 200 mg/kg.
5 % višjega rezultata za vsebnosti zadevnega elementa v sledovih nad 200 mg/kg.
The presence of large quantities of phosphates may interfere with the determination of iron, manganese and zinc.
Če je količina prisotnih fosfatov visoka, lahko moti določanje železa, mangana in cinka.
Such interference must be corrected by addition of lanthanum chloride solution (3.11).
To se mora popraviti z dodajanjem raztopine lantanovega klorida (3.11).
If, however, in the sample the weight ratio Ca + Mg/P is > 2, addition of lanthanum chloride solution (3.11) to the solution for analysis and to the calibration solutions may be omitted.
Če pa je masno razmerju v vzorcu (Ca + Mg/P) > 2, se lahko izpusti dodajanje raztopine lantanovega klorida (3.11) raztopini za analizo in raztopinam za umerjanje.
DETERMINATION OF HALOFUGINONE
DOLOČANJE HALOFUGINONA
DL-trans-7-bromo-6-chloro-3- [3-(3-hydroxy-2-piperidyl)acetonyl]-quinazolin-4-(3H)-one hydrobromide
DL-trans-7-bromo-6-kloro-3-[3-(3-hidroksi-2-piperidil)acetonil]-kvinazolin-4-(3H)-ena hidrobromid
The method makes it possible to determine the level of halofuginone in feed.
Ta metoda omogoča določanje ravni halofuginona v krmi.
The limit of quantification is 1 mg/kg.
Meja kvantifikacije je 1 mg/kg.
After treatment with hot water, halofuginone is extracted as the free base into ethyl acetate and subsequently partitioned as the hydrochloride into an aqueous acid solution.
Po obdelavi z vročo vodo se halofuginon ekstrahira v obliki proste baze v etilni acetat in se nato kot hidroklorid loči v vodno raztopino kisline.
The extract is purified by ion-exchange chromatography.
Ekstrakt se prečisti z ionsko izmenjevalno kromatografijo.
The content of halofuginone is determined by reversed-phase high performance liquid chromatography (HPLC) using an UV detector.
Vsebnost halofuginona se določi s tekočinsko kromatografijo visoke ločljivosti (HPLC) z reverzno fazo in z UV-detektorjem.
Acetonitrile, equivalent to HPLC grade.
Acetonitril, za HPLC
Amberlite XAD-2 resin.
Izmenjevalec Amberlite XAD-2
Ammonium acetate.
Amonijev acetat
Ethyl acetate.
Etilni acetat
Acetic acid, glacial.
Ledocetna kislina
Halofuginone standard substance (DL-trans-7-brome-6-chloro-3-[3-hydroxy-2-piperidyl)acetonyl] quinazoline-4-(3H)-one hydrobromide, E 764).
Standardna snov halofuginon (DL-trans-7-bromo-6-kloro-3-[3-hidroksi-2-piperidil)acetonil]-kvinazolin-4-(3H)-ena hidrobromid, E 764)
Halofuginone stock standard solution, 100 μg/ml
Osnovna standardna raztopina halofuginona, 100 μg/ml
Weight to the nearest 0,1 mg, 50 mg of halofuginone (3.6) in a 500 ml graduated flask, dissolve in ammonium acetate buffer solution (3.18), make up to the mark with the buffer solution and mix.
50 mg halofuginona (3.6) se natehta na 0,1 mg natančno in prenese v 500-mililitrsko merilno bučko ter raztopi v pufrski raztopini amonijevega acetata (3.18), dopolni se s pufrsko raztopino do oznake in premeša.
This solution is stable for three weeks at 5 oC if stored in the dark.
Ta raztopina je stabilna tri tedne pri 5 oC, če se hrani na temnem.
Calibration solutions
Raztopine za umerjanje
Into a series of 100 ml graduated flasks transfer 1,0, 2,0, 3,0, 4,0 and 6,0 ml of the stock standard solution (3.6.1).
V serijo 100-mililitrskih merilnih bučk se prenese 1,0, 2,0, 3,0, 4,0 in 6,0 ml osnovne standardne raztopine (3.6.1).
Make up to the mark with mobile phase (3.21) and mix.
Dopolni se z mobilno fazo (3.21) do oznake in premeša.
These solutions have concentrations of 1,0, 2,0, 3,0, 4,0 and 6,0 μg/ml of halofuginone respectively.
V teh raztopinah je koncentracija halofuginona 1,0, 2,0, 3,0, 4,0 in 6,0 μg/ml.
These solutions must be freshly prepared before use.
Te raztopine je treba pripraviti sveže pred uporabo.
Hydrochloric acid (ρ20 approximately 1,16 g/ml).
Klorovodikova kislina, (ρ20 približno 1,16 g/ml)
Silver nitrate.
Srebrov nitrat
Sodium ascorbate.
Natrijev askorbat
Sodium carbonate.
Natrijev karbonat
EDTA (ethylenediaminetetraacetic acid, disodium salt).
EDTA (etilendiamintetraocetna kislina, dinatrijeva sol)
Water, equivalent to HPLC grade.
Voda, za HPLC
Sodium carbonate solution, c = 10 g/100 ml.
Raztopina natrijevega karbonata, c = 10 g/100 ml
Sodium chloride-saturated sodium carbonate solution, c = 5 g/100 ml.
Raztopina natrijevega klorida in nasičenega natrijevega karbonata, c = 5 g/100 ml
Dissolve 50 g of sodium carbonate (3.11) in water, dilute to 1 litre and add sodium chloride (3.12) until the solution is saturated.
50 g natrijevega karbonata (3.11) se raztopi v vodi, razredči do 1 litra, nato pa se doda natrijev klorid (3.12), dokler raztopina ni nasičena.
Hydrochloric acid, approximately 0,1 mol/l.
Klorovodikova kislina, približno 0,1 mol/l
Dilute 10 ml of HCI (3.7) with water to 1 litre.
10 ml HCl (3.7) se razredči z vodo do 1 litra.
Ammonium acetate buffer solution, approximately 0,25 mol/l.
Pufrska raztopina amonijevega acetata, približno 0,25 mol/l
Dissolve 19,3 g of ammonium acetate (3.3) and 30 ml of acetic acid (3.5) in water (3.14) and dilute to 1 1itre.
19,3 g amonijevega acetata (3.3) in 30 ml ocetne kisline (3.5) se raztopi v vodi (3.14) ter razredči do 1 litra.
Amberlite XAD-2 resin preparation.
Priprava izmenjevalca Amberlite XAD-2
Wash an appropriate quantity of Amberlite (3.2) with water until all chloride ions have been removed, as indicated by a silver nitrate (3.20) test performed on the discarded aqueous phase.
Ustrezna količina Amberlita (3.2) se spira z vodo, dokler niso odstranjeni vsi kloridni ioni, kar dokazuje preskus s srebrovim nitratom (3.20) v zavrženi vodni fazi.
Then wash the resin with 50 ml of methanol (3.8), discard the methanol and store the resin under fresh methanol.
Nato se izmenjevalec spere s 50 ml metanola (3.8), metanol se zavrže, izmenjevalec pa hrani v svežem metanolu.
Silver nitrate solution, approximately 0,1 mol/l.
Raztopina srebrovega nitrata, približno 0,1 mol/l
Dissolve 0,17 g of silver nitrate (3.9) in 10 ml of water.
0,17 g srebrovega nitrata (3.9) se raztopi v 10 ml vode.
HPLC Mobile phase.
Mobilna faza HPLC
Filter through a 0,22 μm filter (4.8) and degas the solution (e.g. by ultrasonification for 10 minutes).
Filtrira se skozi filter 0,22 μm (4.8) in raztopina se razplini (npr. 10 minut v ultrazvočni kopeli).
This solution is stable for one month, if stored in the dark in a closed container.
Ta raztopina je stabilna en mesec, če se hrani na temnem v zaprti posodi.
Rotary film evaporator
Rotacijski uparjalnik
HPLC equipment with variable wavelength ultraviolet detector or diode-array detector
Oprema za HPLC z ultravijoličnim detektorjem z nastavljivo valovno dolžino ali detektorjem s serijo diod
Liquid chromatographic column, 300 mm x 4 mm, C18, 10 μm packaging, or an equivalent column
Kolona za tekočinsko kromatografijo, 300 mm × 4 mm, C18, polnitev 10 μm ali enakovredna kolona
Glass column (300 mm x 10 mm) fitted with a sintered-glass filter and a stopcock
Steklena kolona (300 mm × 10 mm) s filtrom iz sintranega stekla in petelinčkom
Glass-fibre filters, diameter 150 mm
Filtri iz steklenih vlaken, premera 150 mm
Membrane filters, 0,45 μm
Membranski filtri, 0,45 μm
Membrane filters, 0,22 μm
Membranski filtri, 0,22 μm
Halofuginone as the free base is unstable in alkaline and ethyl acetate solutions.
Halofuginon kot prosta baza je nestabilen v bazičnih in etilacetatnih raztopinah.
It shall not remain in ethyl acetate for more than 30 minutes.
V etilacetatu se ne pusti več kot 30 minut.
A blank feed shall be analysed to check that neither halofuginone nor interfering substances are present.
Z analizo slepe krme se preveri, da niso prisotni niti halofuginon niti moteče snovi.
A recovery test shall be carried out by analysing the blank feed which has been fortified by addition of a quantity of halofuginone, similar to that present in the sample.
Preskus izkoristka se izvede z analizo slepe krme, ki se ji doda količina halofuginona, podobna količini v vzorcu.
To fortify at a level of 3 mg/kg, add 300 μl of the stock standard solution (3.6.1) to 10 g of the blank feed, mix and wait for 10 minutes before proceeding with the extraction step (5.2).
Za koncentracijo 3 mg/kg se k 10 g slepe krme doda 300 μl osnovne standardne raztopine (3.6.1), premeša se in počaka 10 minut, preden se začne postopek ekstrakcije (5.2).
for the purpose of this method, the blank feed shall be similar in type to that of the sample and on analysis halofuginone shall not be detected.
Pri tej metodi je vrsta slepe krme podobna vrsti vzorca, pri analizi pa se dokaže odsotnost halofuginona.
Weigh to the nearest 0,1 g, 10 g of the prepared sample, into a 200 ml centrifuge tube, add 0,5 g of sodium ascorbate (3.10), 0,5 g of EDTA (3.13) and 20 ml of water and mix.
10 g pripravljenega vzorca se natehta na 0,1 g natančno in prenese v 200-mililitrsko centrifugirno epruveto, doda se 0,5 g natrijevega askorbata (3.10), 0,5 g EDTA (3.13) in 20 ml vode ter premeša.
Place the tube for 5 minutes in a water bath (80 oC).
Epruveta se postavi za 5 min v vodno kopel (80 oC).
After cooling down to room temperature, add 20 ml of sodium carbonate solution (3.15) and mix.
Po ohladitvi na sobno temperaturo se doda 20 ml raztopine natrijevega karbonata (3.15) in premeša.
Add immediately 100 ml of ethyl acetate (3.4) and shake vigorously by hand for 15 seconds.
Takoj se doda 100 ml etilacetata (3.4) in 15 sekund ročno močno stresa.
Then place the tube for three minutes in the ultrasonic bath (4.1) and loosen the stopper.
Nato se epruveta postavi za 3 minute v ultrazvočno kopel (4.1), zamašek pa zrahlja.
Centrifuge for two minutes and decant the ethyl acetate phase through a glass fibre filter (4.6), into a 500 ml separating funnel.
Centrifugira se 2 minuti, faza etilacetata pa se oddekantira skozi filter iz steklenih vlaken (4.6) v 500-mililitrski lij ločnik.
Repeat the extraction of the sample with a second portion of 100 ml of ethyl acetate.
Ekstrakcija vzorca se ponovi z nadaljnjimi 100 ml etilacetata.
Wash the combined extracts for one minute with 50 ml of sodium chloride saturated sodium carbonate solution (3.16) and discard the aqueous layer.
Sestavljeni ekstrakti se spirajo 1 minuto s 50 ml raztopine nasičenega natrijevega klorida-natrijevega karbonata (3.16), vodna plast pa se zavrže.
Extract the organic layer for 1 min. with 50 ml of hydrochloric acid (3.17).
Organska plast se ekstrahira 1 minuto s 50 ml klorovodikove kisline (3.17).
Run the lower acid layer into a 250 ml separating funnel.
Spodnja kislinska plast se prenese v 250-mililitrski lij ločnik.
Re-extract the organic layer for 1,5 minutes with a further 50 ml of hydrochlorid acid and combine with the first extract.
Organska plast se ponovno ekstrahira 1,5 minute z nadaljnjimi 50 ml klorovodikove kisline, ekstrakt pa se združi s prvim ekstraktom.
Wash the combined acid extracts by swirling for approximately 10 seconds with 10 ml of ethyl acetate (3.4).
Združena ekstrakta se približno 10 sekund spirata z 10 ml etilacetata (3.4) z vrtenjem.
Quantitatively transfer the aqueous layer into a 250 ml round-bottomed flask and discard the organic phase.
Vodna plast se prenese v 250-mililitrsko bučko z okroglim dnom, organska faza pa se zavrže.
Evaporate all the remaining ethyl acetate from the acid solution using a rotary film evaporator (4.2).
Ves preostali etilacetat iz kisle raztopine se upari v rotacijskem uparjalniku (4.2).
The temperature of the water bath must not exceed 40 oC.
Temperatura vodne kopeli ne sme preseči 40 oC.
Under a vacuum of approximately 25 mbar all of the residual ethyl acetate will be removed within 5 minutes at 38 oC.
Ves preostali etilacetat se odstrani v 5 minutah v vakuumu pri približno 25 milibarih in 38 oC.
Clean up
Čiščenje
Preparation of the Amberlite column
Priprava kolone z Amberlitom
An XAD-2 column is prepared for each sample extract.
Kolona XAD-2 se pripravi za vsak ekstrakt vzorca.
Transfer 10 g of prepared Amberlite (3.19) into a glass column (4.5) with methanol (3.8).
10 g pripravljenega Amberlita (3.19) se z metanolom (3.8) prenese v stekleno kolono (4.5).
Add a small plug of glass-wool to the top of the resin bed.
Na vrh posteljice izmenjevalca se položi majhen zamašek iz steklene volne.
Drain the methanol from the column and wash the resin with 100 ml of water, stopping the flow as the liquid reaches the top of the resin bed.
Metanol se izpusti iz kolone, izmenjevalec pa se spere s 100 ml vode tako, da se tok vode ustavi, ko vrh posteljice doseže izmenjevalec.
Allow the column to equilibrate for 10 minutes before use.
Pred uporabo se pusti 10 minut, da se kolona uravnoteži.
Never allow the column to run dry.
Kolona se ne sme nikoli izsušiti.
Sample clean up
Čiščenje vzorca
Transfer the extract (5.2) quantitatively to the top of the prepared Amberlite column (5.3.1) and elute, discarding the eluate.
Ekstrakt (5.2) se prenese na vrh pripravljene kolone z Amberlitom (5.3.1) in eluira, eluat pa se zavrže.
The rate of elution must not exceed 20 ml/min.
Hitrost eluiranja ne sme biti večja od 20 ml/min.
Rinse the round-bottomed flask with 20 ml of hydrochlorid acid (3.17) and use this to wash the resin column.
Bučka z okroglim dnom se spere z 20 ml klorovodikove kisline (3.17) in vsebina se uporabi za spiranje izmenjalne kolone.
Blow through any remaining acid solution with a stream of air.
Kakršna koli preostala raztopina kisline se prepiha z zrakom.
Discard the washings.
Ostanek po izpiranju se zavrže.
Add 100 ml of methanol (3.8) to the column and allow 5 to 10 ml to elute, collecting the eluate in a 250 ml round-bottomed flask.
V kolono se doda 100 ml metanola (3.8), 5–10 ml se eluira, eluat pa se zbere v 250-mililitrsko bučko z okroglim dnom.
Leave the remaining methanol for 10 minutes to equilibrate with the resin and continue the elution at a rate not exceeding 20 ml/min. collecting the eluate in the same round-bottomed flask.
Preostali metanol se pusti 10 minut, da se uravnoteži z izmenjevalcem, eluiranje se nadaljuje s hitrostjo, ki ne presega 20 ml/min, eluat pa se zbira v isto bučko z okroglim dnom.
Evaporate the methanol on the rotary film evaporator (4.2), the temperature of the water bath must not exceed 40 oC.
Metanol se upari na rotacijskem uparjalniku (4.2), pri čemer temperatura vodne kopeli ne sme preseči 40 oC.
Transfer the residue quantitatively into a 10 ml calibrated flask using the mobile phase (3.21).
Ostanek se z mobilno fazo (3.21) prenese v 10-mililitrsko merilno bučko.
Make up to the mark with mobile phase and mix.
Dopolni se z mobilno fazo do oznake in premeša.
An aliquot is filtered through a membrane filter (4.7).
Alikvot se prefiltrira skozi membranski filter (4.7).
Reserve this solution for the HPLC determination (5.4).
Raztopina se shrani za določanje s HPLC (5.4).
The following conditions are offered for guidance, other conditions may be used provided they yield equivalent results.
Naslednji pogoji se lahko uporabljajo kot smernice, lahko pa se uporabljajo drugi pogoji, če zagotavljajo enakovredne rezultate.
Liquid chromatographic column (4.4.1)
Kolona za tekočinsko kromatografijo (4.4.1)
HPLC Mobile phase (3.21)
Mobilna faza HPLC (3.21)
Flow rate: 1,5 to 2 ml/min.
Hitrost pretoka: 1,5–2 ml/min
Detection wavelength: 243 nm
Detekcijska valovna dolžina: 243 nm
Injection volume: 40 to 100 μl.
Volumen, ki se vbrizga: 40 do 100 μl
Check the stability of the chromatographic system, injecting the calibration solution (3.6.2) containing 3,0 μg/ml several times, until constant peak heights (or areas) and retention times are achieved.
Stabilnost kromatografskega sistema se preveri z večkratnim vbrizgavanjem raztopine za umerjanje s koncentracijo 3,0 μg/ml (3.6.2), dokler niso dosežene konstantne višine (ali površine) vrhov in konstantni retencijski časi.
Inject each calibration solution (3.6.2) several times and measure the peak heights (areas) for each concentration.
Vsaka raztopina za umerjanje (3.6.2) se vbrizga večkrat, za vsako koncentracijo pa se izmerijo srednje vrednosti višin (površin) vrhov.
Plot a calibration graph using the mean peak heights or areas of the calibration solutions as the ordinates and the corresponding concentrations in μg/ml as the abscissae.
Umeritvena krivulja se pripravi tako, da se na ordinato nanesejo srednje vrednosti višin ali površin vrhov raztopin za umerjanje, na absciso pa ustrezne koncentracije v μg/ml.
Sample solution
Raztopina vzorca
Inject the sample extract (5.3.2) several times, using the same volume as taken for the calibration solutions and determine the mean peak height (area) of the halofuginone peaks.
Ekstrakt vzorca (5.3.2) se vbrizga večkrat, pri čemer se uporabijo enaki volumni kot za raztopino za umerjanje, nato pa se določi srednja vrednost višin (površin) vrhov halofuginona.
Determine the concentration of the sample solution in μg/ml, from the mean height (area) of the halofuginone peaks of the sample solution by reference to the calibration graph (5.4.2).
Koncentracija raztopine v μg/ml se določi iz srednjih vrednosti višin (površin) vrhov halofuginona v raztopini vzorca iz umeritvene krivulje (5.4.2).
The content of halofuginone w (mg/kg) of the sample is given by the following formula:
Vsebnost halofuginona (w) (mg/kg) v vzorcu se izračuna po naslednji formuli:
c halofuginone concentration of the sample solution in μg/ml,
c koncentracija halofuginona v raztopini vzorca v μg/ml;
m weight of the test portion in grams.
m masa preskušanega vzorca v gramih.
Validation of the results
Potrditev rezultatov
The identity of the analyte can be confirmed by co-chromatography, or by using a diode-array detector by which the spectra of the sample extract and the calibration solution (3.6.2) containing 6,0 μg/ml are compared.
Identiteta analita se lahko potrdi z dodatno kromatografijo ali z detektorjem s serijo diod, s katerim se primerjata spekter ekstrakta vzorca in spekter raztopine za umerjanje (3.6.2) s koncentracijo 6,0 μg/ml.
Co-chromatography
Dodatna kromatografija
A sample extract is fortified by addition of an appropriate amount of a calibration solution (3.6.2).
Ekstraktu vzorca se doda ustrezna količina raztopine za umerjanje (3.6.2).
The amount of added halofuginone must be similar to the estimated amount of halofuginone found in the sample extract.
Količina dodanega halofuginona mora biti podobna ocenjeni količini halofuginona v ekstraktu vzorca.
Only the height of the halofuginone peak shall be enhanced after taking into account both the amount added and the dilution of the extract.
Povišan je le vrh halofuginona ob upoštevanju dodane količine in razredčitve ekstrakta.
The peak width, at half of its maximum height, must be within ± 10 % of the original width.
Širina vrha na polovici maksimalne višine mora biti znotraj ± 10 % prvotne širine.
Diode-array detection
Detekcija s serijo diod
The results are evaluated according to the following criteria:
Rezultati se ocenijo glede na naslednja merila:
the wavelength of maximum absorption of the sample and of the standard spectra, recorded at the peak apex on the chromatogram, must be the same within a margin determined by the resolving power of the detection system.
valovni dolžini maksimalne absorpcije spektra vzorca in spektra standarda, zabeleženi na najvišji točki vrha na kromatogramu, morata biti znotraj meja, določenih z ločljivostjo sistema za detekcijo.
For diode-array detection, this is typically within ± 2 nm;
Za detekcijo s serijo diodo je to ponavadi znotraj ± 2 nm;
between 225 and 300 nm, the sample and standard spectra recorded at the peak apex on the chromatogram, must not be different for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
med 225 in 300 nm se spekter vzorca in spekter standarda, zabeležena na najvišji točki kromatografskega vrha, za navedene dele spektra ne smeta razlikovati znotraj območja 10–100 % relativne absorbance.
This criterion is met when the same maxima are present and at no observed point the deviation between the two spectra exceeds 15 % of the absorbance of the standard analyte;
To merilo je izpolnjeno, ko so prisotne iste najvišje vrednosti in ko odmik med dvema spektroma na nobeni opazovani točki ne preseže 15 % absorbance standardnega analita;
between 225 and 300 nm, the spectra of the upslope, apex and downslope of the peak produced by the sample extract must not be different from each other for those parts of the spectrum within the range 10 % to 100 % of relative absorbance.
med 225 in 300 nm se spektri naraščanja, najvišje točke vrha in padanja vrha, pridobljeni iz ekstrakta vzorca, za navedene dele spektra ne smejo razlikovati znotraj območja 10–100 % relativne absorbance.
This criterion is met when the same maxima are present and when at all observed points the deviation between the spectra does not exceed 15 % of the absorbance of the spectrum of the apex.
To merilo je izpolnjeno, ko so prisotni iste najvišje točke in ko odmik med spektri na nobeni od opazovanih točk ne preseže 15 % absorbance spektra najvišje točke.
If one of these criteria is not met the presence of the analyte has not been confirmed.
Če eno od teh meril ni izpolnjeno, prisotnost analita ni potrjena.
The difference between results of two parallel determinations carried out on the same sample must not exceed 0,5 mg/kg for halofuginone contents up to 3 mg/kg.
Razlika med rezultatoma dveh vzporednih določanj, izvedenih na istem vzorcu, ne sme presegati 0,5 mg/kg za vsebnost halofuginona do 3 mg/kg.
For the fortified blank sample the recovery shall be at least 80 %.
Za slepi vzorec z dodatkom je izkoristek najmanj 80 %.
(blank)
(slepi)
On receipt
ob prejemu
Sample B (Meal)
Vzorec B (v prahu)
Sample C (Pellets)
Vzorec C (v briketih)
After two months
po 2 mesecih
Mean [mg/kg]
Srednja vrednost [mg/kg]
ND not detected
ND ni zaznano;
coefficient of variation of reproducibility (%)
koeficient variacije obnovljivosti (%);
Rec. recovery (%)
Rec. izkoristek (%).
DETERMINATION OF ROBENIDINE
DOLOČANJE ROBENIDINA
1,3-bis [(4-chlorobenzylidene)amino]guanidine — hydrochloride
1,3-bis [(4-klorobenziliden)amino]gvanidin – hidroklorid
This method makes it possible to determine the levels of robenidine in feed.
Ta metoda omogoča določanje ravni robenidina v krmi.
The limit of quantification is 5 mg/kg.
Meja kvantifikacije je 5 mg/kg.
The sample is extracted with acidified methanol.
Vzorec se ekstrahira z nakisanim metanolom.