natgillin/translations-raw huggingface dataset:
Calculate the mean peak heights (areas) of several injections of the same sample solution and the mean peak heights (areas) of several injections of the calibration solutions (5.6.2).
Izračunajo se srednje vrednosti višin (površin) vrhov za več vbrizgov iste raztopine vzorca in srednje vrednosti višin (površin) vrhov za več vbrizgov raztopin za umerjanje (5.6.2).
Mobile phase (3.8):
Mobilna faza (3.8):
Fluorescence detector
Fluorescečni detektor:
(excitation: 295 nm/emission: 330 nm) or UV detector (292 nm)
(ekscitacija: 295 nm/emisija: 330 nm) ali UV-detektor (292 nm)
Calibration (DL-α-tocopherol acetate or DL-α-tocopherol)
Umerjanje (acetat DL-α-tokoferola ali DL-α-tokoferol)
DL-α-tocopherol acetate standard
Standardni acetat DL-α-tokoferola
Preparation of the working standard solution
Priprava delovne standardne raztopine
Transfer by pipette 25 ml of the DL-α-tocopherol acetate stock solution (3.10.1) into a 500 ml flat bottom or conical flask (4.2.1) and hydrolyse as described under 5.2.
25 ml osnovne raztopine acetata DL-α-tokoferola (3.10.1) se odpipetira v 500-mililitrsko bučko z ravnim dnom ali erlenmajerico (4.2.1) in hidrolizira, kakor je opisano v točki 5.2.
Subsequently extract with light petroleum (3.2) according to 5.3 and make up to 500 ml with light petroleum.
Nato se ekstrahira s petroletrom (3.2) v skladu s točko 5.3 in dopolni do 500 ml s petroletrom.
Evaporate 25 ml of this extract on the rotary evaporator (see 5.4) nearly to dryness, remove the remaining solvent with a stream of nitrogen (3.9) and redissolve the residue in 25,0 ml of methanol (3.3).
25 ml tega ekstrakta se upari v rotacijskem uparjalniku (glej 5.4) skoraj do suhega, odstrani se preostalo topilo s tokom dušika (3.9), ostanek pa se ponovno raztopi v 25,0 ml metanola (3.3).
The nominal concentration of this solution is 45,5 μg DL-α-tocopherol per ml, equivalent to 50 μg DL-α-tocopherol acetate per ml.
Nominalna koncentracija te raztopine je 45,5 μg DL-α-tokoferola na ml, kar je enakovredno 50 μg acetata DL-α-tokoferola na ml.
Transfer 1,0, 2,0, 4,0 and 10,0 ml of the working standard solution into a series of 20 ml graduated flasks, make up to the mark with methanol (3.3) and mix.
1,0, 2,0, 4,0 in 10,0 ml delovne standardne raztopine se prenese v serijo 20-mililitrskih merilnih bučk, dopolni se do oznake z metanolom (3.3) in premeša.
The nominal concentrations of these solutions are 2,5, 5,0, 10,0 and 25,0 μg/ml DL-α-tocopherol acetate, i.e. 2,28, 4,55, 9,10 μg/ml and 22,8 μg/ml DL-α-tocopherol.
Nominalne koncentracije teh raztopin so 2,5, 5,0, 10,0 in 25,0 μg/ml acetata DL-α-tokoferola, tj. 2,28, 4,55, 9,10 in 22,8 μg/ml DL-α-tokoferola.
Using the mean peak heights (areas) plot a calibration graph.
Iz srednjih vrednosti višin (površin) vrhov se pripravi umeritvena krivulja.
UV standardisation of the DL-α-tocopherol acetate stock solution (3.10.1)
UV-standardizacija osnovne raztopine acetata DL-α-tokoferola (3.10.1)
Dilute 5,0 ml of the DL-α-tocopherol acetate stock solution (3.10.1) to 25,0 ml with ethanol and measure the UV spectrum of this solution against ethanol (3.1) in the spectrophotometer (4.6) between 250 nm and 320 nm.
5,0 ml osnovne raztopine acetata DL-α-tokoferola (3.10.1) se razredči na 25,0 ml z etanolom, UV-spekter te raztopine pa se izmeri proti etanolu (3.1) v spektrofotometru (4.6) med 250 in 320 nm.
The absorption maximum shall be at 284 nm:
Maksimalna absorpcija je pri 284 nm:
= 43,6 at 284 nm in ethanol
= 43,6 pri 284 nm v etanolu
At this dilution an extinction value of 0,84 to 0,88 must be obtained.
Pri tem razredčenju mora biti vrednost ekstinkcije 0,84 do 0,88.
Transfer by pipette 2 ml of the DL-α-tocopherol stock solution (3.11.1) into a 50 ml graduated flask, dissolve in methanol (3.3) and make up to the mark with methanol.
2 ml osnovne raztopine DL-α-tokoferola (3.11.1) se odpipetira v 50-mililitrsko merilno bučko, raztopi v metanolu (3.3) in dopolni z metanolom do oznake.
The nominal concentration of this solution is 40 μg DL-α-tocopherol per ml, equivalent to 44,0 μg DL-α-tocopherol acetate per ml.
Nominalna koncentracija te raztopine je 40 μg DL-α-tokoferola na ml, kar je enakovredno 44,0 μg acetata DL-α-tokoferola na ml.
The nominal concentrations of these solutions are 2,0, 4,0, 8,0 and 20,0 μg/ml DL-α-tocopherol, i.e. 2,20, 4,40, 8,79 μg/ml and 22,0 μg/ml DL-α-tocopherol acetate.
Nominalne koncentracije teh raztopin so 2,0, 4,0, 8,0 in 20,0 μg/ml DL-α-tokoferola, tj. 2,20, 4,40, 8,79 in 22,0 μg/ml acetata DL-α-tokoferola.
UV standardisation of the DL-α-tocopherol stock solution (3.11.1)
UV-standardizacija osnovne raztopine DL-α-tokoferola (3.11.1)
Dilute 2,0 ml of the DL-α-tocopherol stock solution (3.11.1) to 25,0 ml with ethanol and measure the UV spectrum of this solution against ethanol (3.1) in the spectrophotometer (4.6) between 250 nm and 320 nm.
2,0 ml osnovne raztopine DL-α-tokoferola (3.11.1) se razredči na 25,0 ml z etanolom, UV-spekter te raztopine pa se izmeri proti etanolu (3.1) v spektrofotometru (4.6) med 250 in 320 nm.
The absorption maximum shall be at 292 nm:
Maksimalna absorpcija je pri 292 nm:
= 75,8 at 292 nm in ethanol
= 75,8 pri 292 nm v etanolu
At this dilution an extinction value of 0,6 must be obtained.
Pri tem razredčenju mora biti vrednost ekstinkcije 0,6.
From the mean height (area) of the vitamin E peaks of the sample solution determine the concentration of the sample solution in μg/ml (calculated as α-tocopherol acetate) by reference to the calibration graph (5.6.1.2 or 5.6.2.2).
Iz srednje vrednosti višine (površine) vrhov vitamina E iz raztopine vzorca se s pomočjo umeritvene krivulje (5.6.1.2 ali 5.6.2.2) določi koncentracija raztopine vzorca v μg/ml (izračunana kot acetat DL-α-tokoferola).
The vitamin E content w in mg/kg of the sample is given by the following formula:
Vsebnost vitamina E (w) v mg/kg v vzorcu se izračuna po naslednji formuli:
c vitamin E concentration (as α-tocopherol acetate) of the sample solution (5.4) in μg/ml
c koncentracija vitamina E (kot acetata α-tokoferola) v raztopini vzorca (5.4) v μg/ml;
volume of sample solution (5.4), in ml
volumen raztopine vzorca (5.4) v ml;
volume of aliquot taken in (5.4), in ml
volumen alikvota, uporabljenega pri točki 5.4, v ml;
For samples with low vitamin E concentration it may be useful to combine the light petroleum-extracts of two saponification-charges (amount weighed: 25 g) to one sample solution for HPLC-determination.
Pri vzorcih z nizko koncentracijo vitamina E se ekstrakti petroletra iz dveh umilitvenih šarž (natehtana množina: 25 g) združijo v eno raztopino vzorca za določanje s HPLC.
After the spectrophotometric measurement of the DL-α-tocopherol acetate or DL-α-tocopherol solution according to 5.6.1.3 or 5.6.2.3 respectively add approximately 10 mg BHT (3.12) to the solution (3.10.1 or 3.10.2) and keep the solution in a refrigerator (storage life max. 4 weeks).
Po spektrofotometrični meritvi raztopine acetata DL-α-tokoferola ali raztopine DL-α-tokoferola v skladu s točko 5.6.1.3 ali pa 5.6.2.3 se doda približno 10 mg BHT (3.12) v raztopino (3.10.1 ali 3.10.2), raztopina pa se hrani v hladilniku (hrani se lahko največ 4 tedne).
Using a normal phase-column the separation of α-, β-, γ- and δ-tocopherol is possible.
Z običajno fazno kolono je možno ločiti α-, β-, γ- in δ-tokoferol.
Vitamin E acetate hydrolyses very fast under alkaline conditions and is therefore very sensitive to oxidation, especially in the presence of trace elements like iron or copper.
Acetat vitamina E se v bazičnih pogojih hidrolizira zelo hitro, zato je zelo občutljiv na oksidacijo, zlasti v navzočnosti elementov v sledovih, kot sta železo in baker.
Repeatability·
Ponovljivost
mean [mg/kg]
Srednja vrednost [mg/kg]
coefficient of variation of reproducibility
koeficient variacije obnovljivosti.
DETERMINATION OF THE TRACE ELEMENTS IRON, COPPER, MANGANESE AND ZINC
DOLOČANJE ŽELEZA, BAKRA, MANGANA IN CINKA V SLEDOVIH
The method makes it possible to determine the trace elements iron, copper, manganese and zinc in feed.
Ta metoda omogoča določanje železa, bakra, mangana in cinka v sledovih v krmi.
The limits of quantification are:
Meje za določanje so:
iron (Fe): 20 mg/kg
železo (Fe): 20 mg/kg,
copper (Cu): 10 mg/kg
baker (Cu): 10 mg/kg,
manganese (Mn): 20 mg/kg
mangan (Mn): 20 mg/kg,
zinc (Zn): 20 mg/kg.
cink (Zn): 20 mg/kg.
The sample is brought into solution in hydrochloric acid after destruction of organic matter, if any.
Po razkroju morebitnih organskih snovi se vzorec raztopi v klorovodikovi kislini.
The elements iron, copper, manganese and zinc are determined, after appropriate dilution, by atomic absorption spectrometry.
Po primernem razredčenju se z atomsko absorpcijsko spektrometrijo določijo železo, baker, mangan in cink.
Introductory comments
Uvodne opombe
For preparation of the reagents and analytical solutions use water free from the cations to be determined, obtained either by double distilling water in a borosilicate glass or quartz still or by double treatment on ion exchange resin.
Za pripravo reagentov in raztopin za analizo se uporabi voda brez kationov, ki jih je treba določiti, ki se pripravi z dvojnim destiliranjem vode v destilatorju iz borosilikatnega ali kremenovega stekla ali z dvojno ionsko izmenjavo.
The reagents must be of at least analytical grade.
Reagenti morajo biti najmanj analitske čistoče.
Freedom from the element to be determined must be checked in a blank experiment.
Odsotnost elementa, ki se določa, se preveri s slepim preskusom.
If necessary, the reagents must be further purified.
Po potrebi se morajo reagenti dodatno prečistiti.
In place of the standard solutions described below, commercial standard solutions may be used provided that they are guaranteed and have been checked before use.
Namesto spodaj navedenih standardnih raztopin se lahko uporabijo komercialne standardne raztopine, če imajo jamstvo in so bile pred uporabo preverjene.
Hydrochloric acid (d:1,19 g/ml).
Klorovodikova kislina (g: 1,19 g/ml)
Hydrochloric acid (6 mol/litre).
Klorovodikova kislina (6 mol/l)
Hydrochloric acid (0,5 mol/litre).
Klorovodikova kislina (0,5 mol/l)
Hydrofluoric acid 38 % to 40 % (v/v) having an iron (Fe) content of less than 1 mg/litre and a residue after evaporation of less than 10 mg (as sulphate)/litre.
38–40-odstotna (v/v) klorovodikova kislina z vsebnostjo železa (Fe) manj kot 1 mg/l in z manj kot 10 mg/l ostanka (v obliki sulfata) po uparevanju
Sulphuric acid (d: 1,84 g/ml).
Žveplova kislina (g: 1,84 g/ml)
Hydrogen peroxide (approximately 100 volumes of oxygen (30 % by weight)).
Vodikov peroksid a.č. (približno 100 volumnov kisika (30 % po masi))
Standard iron solution (1000 μg Fe/ml) prepared as follows or equivalent commercially available solution: dissolve 1 g of iron wire in 200 ml of 6 mol/litre hydrochloric acid (3.2), add 16 ml of hydrogen peroxide (3.6) and make up to one litre with water.
Standardna raztopina železa (1000 μg Fe/ml), pripravljena na naslednji način, ali enakovredna komercialno dostopna raztopina: 1 g železne žice se raztopi v 200 ml klorovodikove kisline 6 mol/l (3.2), doda se 16 ml vodikovega peroksida (3.6) in dopolni z vodo do 1 litra.
Working standard iron solution (100 μg Fe/ml) prepared by diluting one part of the standard solution (3.7) with 9 parts of water.
Standardna delovna raztopina železa (100 μg Fe/ml), pripravljena z redčenjem 1 dela standardne raztopine (3.7) z 9 deli vode.
Standard copper solution (1000 μg Cu/ml) prepared as follows or equivalent commercially available solution:
Standardna raztopina bakra (1000 μg Cu/ml), pripravljena na naslednji način, ali enakovredna komercialno dostopna raztopina:
dissolve 1 g of copper in powder form in 25 ml of 6 mol/litre hydrochloric acid (3.2), add 5 ml of hydrogen peroxide (3.6) and make up to one litre with water.
1 g bakra v prahu se raztopi v 25 ml klorovodikove kisline 6 mol/l (3.2), doda se 5 ml vodikovega peroksida (3.6) in dopolni z vodo do 1 litra.
Working standard copper solution (10 μg Cu/ml) prepared by diluting 1 part of the standard solution (3.8) with 9 parts of water and then diluting 1 part of the resulting solution with 9 parts of water.
Standardna delovna raztopina bakra (10 μg Cu/ml), pripravljena z redčenjem 1 dela standardne raztopine (3.8) z 9 deli vode, nato pa z redčenjem 1 dela te raztopine z 9 deli vode.
Standard manganese solution (1000 μg Mn/ml) prepared as follows or equivalent commercially available solution:
Standardna raztopina mangana (1000 μg Mn/ml), pripravljena na naslednji način, ali enakovredna komercialno dostopna raztopina:
dissolve 1 g of manganese in powder form in 25 ml of 6 mol/litre hydrochloric acid (3.2) and make up to one litre with water.
1 g mangana v prahu se raztopi v 25 ml klorovodikove kisline 6 mol/l (3.2) in dopolni z vodo do 1 litra.
Working standard manganese solution (10 μg Mn/ml) prepared by diluting 1 part of the standard solution (3.9) with 9 parts of water and then diluting 1 part of the resulting solution with 9 parts of water.
Standardna delovna raztopina mangana (10 μg Mn/ml), pripravljena z redčenjem 1 dela standardne raztopine (3.9) z 9 deli vode, nato pa z redčenjem 1 dela te raztopine z 9 deli vode.
Standard zinc solution (1000 μg Zn/ml) prepared as follows or equivalent commercially available solution:
Standardna raztopina cinka (1000 μg Zn/ml), pripravljena na naslednji način, ali enakovredna komercialno dostopna raztopina:
dissolve 1 g of zinc in strip or leaf form in 25 ml of 6 mol/litre hydrochloric acid (3.2) and make up to one litre with water.
1 g cinka v foliji ali granulah se raztopi v 25 ml klorovodikove kisline 6 mol/l (3.2) in dopolni z vodo do 1 litra.
Working standard zinc solution (10 μg Zn/ml) prepared by diluting 1 part of the standard solution (3.10) with 9 parts of water and then diluting 1 part of the resulting solution with 9 parts of water.
Standardna delovna raztopina cinka (10 μg Zn/ml), pripravljena z redčenjem 1 dela standardne raztopine (3.10) z 9 deli vode, nato pa z redčenjem 1 dela te raztopine z 9 deli vode.
Lanthanum chloride solution: dissolve 12 g of lanthanum oxide in 150 ml of water, add 100 ml of 6 mol/litre hydrochloric acid (3.2) and make up to one litre with water.
Raztopina lantanovega klorida: 12 g lantanovega klorida se raztopi v 150 ml vode, doda se 100 ml klorovodikove kisline 6 mol/l (3.2) in dopolni z vodo do 1 litra.
Muffle furnace with temperature regulation and preferably recorder.
Žarilna peč z regulacijo temperature in po možnosti rekorderjem
Glassware must be of resistant borosilicate type and it is recommended to use apparatus which is reserved exclusively for trace element determinations.
Stekleni deli morajo biti iz odpornega borosilikata, priporočljiva pa je uporaba opreme, ki je namenjena izključno določanju elementov v sledovih.
Atomic absorption spectrophotometer meeting the requirements of the method with regard to sensitivity and precision in the required range.
Atomski absorpcijski spektrofotometer, ki ustreza zahtevam metode glede občutljivosti in natančnosti zahtevanega območja.
Samples containing organic matter
Vzorci, ki vsebujejo organske snovi
Ashing and preparation of the solution for analysis
Upepeljevanje in priprava raztopine za analizo
Place 5 to 10 g of sample weighed to the nearest 0,2 mg in a quartz or platinum crucible (see Note (b)), dry in an oven at 105 oC and introduce the crucible into the cold muffle furnace (4.1).
5–10 g vzorca se natehta na 0,2 mg natančno in prenese v žarilni lonček iz kvarca ali platine (glej opombo (b)), osuši v sušilniku pri 105 oC in postavi v hladno žarilno peč (4.1).
Close the furnace (see Note (c)) and gradually raise the temperature to 450 to 475 oC over about 90 minutes.
Peč se zapre (glej opombo (c)), temperatura pa se približno 90 minut postopoma viša na 450–475 oC.
Maintain this temperature for 4 to 16 hours (e.g. overnight) to remove carbonaceous material and then open the furnace and allow to cool (see Note (d)).
Ta temperatura se ohranja 4–16 ur (npr. čez noč), da se odstranijo zoglenele snovi, nato pa se peč odpre in ohladi (glej opombo (d)).
Moisten the ashes with water and transfer these in a beaker of 250 ml.
Pepel se navlaži z vodo in prenese v 250-mililitrsko čašo.
Wash the crucible out with a total of about 5 ml of hydrochloric acid (3.1) and add the latter slowly and carefully to the beaker (there may be a vigorous reaction due to CO2 formation).
Lonček se spere s skupaj približno 5 ml klorovodikove kisline (3.1), vsebina pa se počasi in previdno prenese v čašo (lahko pride do burne reakcije zaradi nastanka CO2).
Add hydrochloric acid (3.1) dropwise with agitation until all effervescence has stopped.
Po kapljicah se dodaja klorovodikova kislina (3.1), meša pa se, dokler penjenje ne poneha.
Evaporate to dryness, occasionally stirring with a glass rod.
Upareva se do suhega, pri čemer se občasno premeša s stekleno palčko.
Next add 15 ml of 6 mol/litre hydrochloric acid (3.2) to the residue followed by about 120 ml of water.
Nato se ostanku doda 15 ml klorovodikove kisline 6 mol/l (3.2) in približno 120 ml vode.
Stir with the glass rod, which shall be left in the beaker, and cover the beaker with a watch-glass.
Premeša se s stekleno paličico, ki se pusti v čaši, čaša pa se pokrije z urnim steklom.
Bring gently to the boil and maintain at boiling point until no more ash can be seen to dissolve.
Počasi se segreva do vretja in pusti vreti do popolne raztopitve pepela.
Filter on ash-free filter paper and collect the filtrate in a 250 ml volumetric flask.
Skozi filter, ki ne vsebuje pepela, se filtrira v 250-mililitrsko merilno bučko.
Wash the beaker and filter with 5 ml of hot 6 mol/litre hydrochloric acid (3.2) and twice with boiling water.
Čaša in filter se spereta s 5 ml vroče klorovodikove kisline 6 mol/l (3.2) in dvakrat z vrelo vodo.
Fill the volumetric flask up to the mark with water (HCl concentration about 0,5 mol/litre).
Merilna bučka se napolni z vodo do oznake (koncentracija HCl približno 0,5 mol/l).
If the residue in the filter appears black (carbon), put it back in the furnace and ash again at 450 to 475 oC.
Če je ostanek na filtru videti črn (ogljik), se ponovno postavi v peč in upepeli pri 450–475 oC.
This ashing, which only requires a few hours (about three to five hours), is complete when the ash appears white or nearly white.
Ta upepelitev, ki zahteva le nekaj ur (približno 3–5 ur), je končana, ko je pepel videti bel ali skoraj bel.
Dissolve the residue with about 2 ml of hydrochloric acid (3.1), evaporate to dryness and add 5 ml of 6 mol/litre hydrochloric acid (3.2).
Ostanek se raztopi s približno 2 ml klorovodikove kisline (3.1), upari do suhega in doda 5 ml klorovodikove kisline 6 mol/l (3.2).
Heat, filter the solution into the volumetric flask and make up to the mark with water (HCl concentration about 0,5 mol/litre.
Segreje se, raztopina se filtrira v merilno bučko in dopolni z vodo do oznake (koncentracija HCl približno 0,5 mol/l).
In determining trace elements it is important to be alert to the risks of contamination, particularly by zinc, copper and iron.
Pri določanju elementov v sledovih je pomembno paziti zaradi tveganja onesnaženja, predvsem s cinkom, bakrom in železom.
For this reason, the equipment used in preparing the samples must be free of these metals.
Zato oprema, ki se uporablja pri pripravi vzorcev, ne sme vsebovati teh kovin.
To reduce the general risk of contamination, work in a dust-free atmosphere with scrupulously clean equipment and carefully washed glassware.
Za zmanjšanje splošnega tveganja onesnaženja je treba delati v brezprašnem prostoru s popolnoma čisto opremo in dobro pomitimi steklenimi posodami.
The determination of zinc is particularly sensitive to many types of contamination, e.g. from glassware, reagents, dust, etc.
Določanje cinka je posebej občutljivo na več vrst onesnaženja, npr. s stekleno posodo, reagenti, prahom itd.
The weight of sample to be ashed is calculated from the approximate trace element content of the feed in relation to the sensitivity of the spectrophotometer used.
Masa vzorca, ki se upepeli, se izračuna iz približne vsebnosti elementov v sledovih v krmi glede na občutljivost uporabljenega spektrofotometra.
For certain feed low in trace elements it may be necessary to start with a 10 to 20 g sample and make up the final solution to only 100 ml.
Pri nekateri krmi z nizko vsebnostjo elementov v sledovih je morda potrebno začeti z 10–20 g vzorca ter končno raztopino dopolniti do samo 100 ml.
Ashing must be carried out in a closed furnace without injection of air or oxygen.
Upepeljevanje se mora izvajati v zaprti peči brez dovajanja zraka ali kisika.
The temperature indicated by the pyrometer must not exceed 475 oC.
Temperatura na pirometru ne sme preseči 475 oC.
Spectrophotometric determination
Določanje s spektrofotometrijo
Preparation of calibration solutions
Priprava raztopin za umerjanje
For each of the elements to be determined, prepare from the working standard solutions given in points 3.7.1, 3.8.1, 3.9.1 and 3.10.1 a range of calibration solutions, each calibration solution having an HCl concentration of about 0,5 mol/litre (and (in the cases of iron, manganese and zinc) a lanthanum chloride concentration equivalent to 0,1 % La (w/v).
Za vsak element, ki se določa, se iz standardnih delovnih raztopin iz točk 3.7.1, 3.8.1, 3.9.1 in 3.10.1 pripravi serija raztopin za umerjanje, pri čemer je koncentracija HCl v vsaki približno 0,5 mol/l (in (pri železu, manganu in cinku) koncentracija lantanovega klorida, ki ustreza 0,1 % La (m/v)).
The trace element concentrations selected must lie within the range of sensitivity of the spectrophotometer used.
Izbrane koncentracije elementov v sledovih morajo ustrezati območju občutljivosti uporabljenega spektrofotometra.
The tables below show, by way of example, the compositions of typical ranges of calibration solutions; depending, however, on the type and sensitivity of spectrophotometer used it may be necessary to select other concentrations.
V spodnjih preglednicah so kot primer navedena tipična območja raztopin za umerjanje glede na vrsto in občutljivost uporabljenega spektrofotometra, zato je morda treba izbrati drugačne koncentracije.
ml working standard solution (3.7.1) (1 ml = 100 μg Fe)
ml delovne standardne raztopine (3.7.1) (1 ml = 100 μg Fe)
+ 10 ml of lanthanum chloride solution (3.11) and make up to 100 ml with water
+ 10 ml raztopine lantanovega klorida (3.11) in dopolniti z vodo do 100 ml
ml working standard solution (3.8.1) (1 ml = 10 μg Cu)
ml delovne standardne raztopine (3.8.1) (1 ml = 10 μg Cu)
ml working standard solution (3.9.1) (1 ml = 10 μg Mn)
ml delovne standardne raztopine (3.9.1) (1 ml = 10 μg Mn)
ml working standard solution (3.10.1) (1 ml = 10 μg Zn)
ml delovne standardne raztopine (3.10.1) (1 ml = 10 μg Zn)
Preparation of solution for analysis
Priprava raztopine za analizo
For the determination of copper, the solution prepared from point 5.1.1 can normally be used directly.
Za določanje bakra se lahko raztopina, pripravljena v skladu s točko 5.1.1, ponavadi uporabi neposredno.
If necessary to bring its concentration within the range of the calibration solutions, an aliquot portion may be pipetted into a 100 ml volumetric flask and made up to the mark with 0,5 mol/litre hydrochloric acid (3.3).
Če je treba koncentracijo prilagoditi območju umeritve, se ustrezen alikvot odpipetira v 100-mililitrsko merilno bučko in dopolni s klorovodikovo kislino 0,5 mol/l (3.3) do oznake.
For the determination of iron, manganese and zinc, pipette an aliquot portion of the solution prepared from point 5.1.1 into a 100 ml volumetric flask, add 10 ml of lanthanum chloride solution (3.11) and make up to the mark with 0,5 mol/litre hydrochloric acid (3.3) (see also point 8 ‘Observation’).
Za določanje železa, mangana in cinka se odpipetira ustrezen alikvot raztopine, pripravljene v skladu s točko 5.1.1, v 100-mililitrsko merilno bučko, doda se 10 ml raztopine lantanovega klorida (3.11) in dopolni do oznake s klorovodikovo kislino 0,5 mol/l (3.3) (glej tudi točko 8 „Opomba“).
Blank experiment
Slepi preskus
The blank experiment must include all the prescribed steps of the procedure except that the sample material is omitted.
Slepi preskus mora vključevati vse predpisane korake postopka, le da se izpusti vzorec.