natgillin/translations-raw huggingface dataset:
concentration of the hydrochloric acid solution (M)
koncentracija raztopina klorovodikove kisline (M),
if CB is 0,0125 M and CA is 0,05 M, the titration for 100 ml barium hydroxide is 50 ml and the weight of CO2 is given by:
če je CB enak 0,0125 M in CA enak 0,05 M, se za titracijo 100 ml barijevega hidroksida porabi 50 ml, je masa CO2:
× 44 × ml HCI titrated = 1,1 × ml HCI
× 44 × ml HCI porabljeni pri titraciji = 1,1 × ml HCI
Thus, in this case, to convert volume of HCl titrated to mg CO2 produced the factor is 1,1.
V tem primeru je torej faktor za pretvorbo pri titraciji porabljene HCl v mg CO2 enak 1,1.
Calculate the weights of CO2 produced from the inoculum alone and from the inoculum plus test chemical using the respective titration values and the difference is the weight of CO2 produced from the test chemical alone.
Iz ustreznih vrednosti, dobljenih pri titraciji, izračunamo maso CO2, proizvedenega samo iz inokuluma ter iz inokuluma in preskusne snovi; razlika je masa CO2, proizvedenega iz preskusne snovi.
For example, if the inoculum alone gives a titration of 48 ml and inoculum plus test chemical gives 45 ml,
Če na primer pri vzorcu z inokulumom porabimo za titracijo 48 ml ter pri vzorcu z inokulumom in preskusno snovjo 45 ml,
from inoculum = 1,1 × (50-48) = 2,2 mg
iz inokuluma = 1,1 × (50 - 48) = 2,2 mg
from inoculum plus test chemical = 1,1 × (50-45) = 5,5 mg
iz inokuluma in preskusne snovi skupaj = 1,1 × (50 - 45) = 5,5 mg
and thus the weight of CO2 produced from the test chemical is 3,3 mg.
torej je masa CO2, proizvedenega iz preskusne snovi, enaka 3,3 mg.
The percentage biodegradation is calculated from:
Odstotek biorazgradnje se izračuna po enačbi:
% degradation=mg CO2
% razgradnje=mg proizvedenega CO2
ThCO2
TCO2
× mg test chemical added ,
× mg dodane preskusne snovi
% degradation =mg CO2
% razgradnje =mg proizvedenega CO2
mg TO added in test × 3,67
mg Tv preskusu dodanega TCO × 3,67
3,67 being the conversion factor (44/12) for carbon to carbon dioxide.
pri čemer je 3,67 faktor pretvorbe (44/12) ogljika v ogljikov dioksid.
Obtain the percentage degradation after any time interval by adding the percentage of ThCO2 values calculated for each of the days, up to that time, on which it was measured.
Odstotek razgradnje po vsakem časovnem intervalu določimo tako, da seštejemo odstotke TCO2, izračunane za vsak dan do dneva meritve.
For sodium hydroxide absorbers, calculate the amount of carbon dioxide prodllced, expressed as IC (mg), by multiplying the concentration of IC in the absorbent by the volume of the absorbent.
Pri absorberjih z natrijevim hidroksidom, količino proizvedenega ogljikovega dioksida, izraženega kot anorganski ogljik IC (mg), izračunamo tako, da koncentracijo IC v absorbentu pomnožimo z volumnom absorbenta.
Calculate the percentage degradation from:
Odstotek razgradnje izračunamo po enačbi:
% of ThCO2
% TCO2
=mg IC flask - mg IC blank
=mg IC v preskusni steklenici – mg IC v slepem vzorcu
MG TOC added as test chemical
MG TOC dodanega kot preskusna snov
Calculate DOC removals (optional) as described under I.7.
Odstranitev DOC (neobvezno) izračunamo, kakor je opisano v I.7.
Record these and all other results on the data sheets provided.
Te in vse druge rezultate zapišemo na predvidene podatkovne liste.
The IC content of the test chemical suspension in the mineral medium at the beginning of the test must be less than 5 % of the TC, and the total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/1 medium.
Vsebnost IC v suspenziji preskusne snovi v mineralnem mediju mora biti na začetku preskusa manj kot 5 % TC in skupni CO2, razvit v slepem vzorcu z inokulumom, na koncu preskusa ne sme preseči 40 mg/l medija.
If values greater than 70 mg CO2/litre are obtained, the data and experimental technique should be examined critically.
Če dobljene vrednosti presežejo 70 mg CO2/liter, je treba kritično preveriti podatke in tehniko preskušanja.
See also I.5.2.
Glej tudi 1.5.2.
CARBON DIOXIDE EVOLUTION TEST
PRESKUS RAZVIJANJA OGLJIKOVEGA DIOKSIDA
Initial conc. in medium: … mg/litre as chemical
Začetna konc. v mediju: ... mg/l kot snov
Total C added to flask: … mg C
Skupni C, dodan v steklenico: ... mg C
ThCO2: mg CO2
TCO2: ... mg CO2
Pre-conditioning if any:
Aklimatizacija, če je bila opravljena:
Concentration of suspended solids in reaction mixture: mg/litre
PROIZVODNJA OGLJIKOVEGA DIOKSIDA IN RAZGRADLJIVOST Metoda Ba(OH)/NaOH/drugo
(day)
(dan)
mean
srednja vrednost
Note: similar formats may be used for the reference chemical and toxicity controls.
Opomba: za referenčno snov in vzorce za kontrolo strupenosti se lahko uporabi podobna oblika.
CARBON ANALYSIS (optional)
ANALIZA OGLIJIKA (neobvezno):
Time (day)
Čas (dan)
Blank mg/l
Slepi vzorec mg/l
Test chemical mg/l
Preskusna snov mg/l
% DOC removed = 1 - Ct
% odstranjenega DOC = 1 - Ct
ABIOTIC DEGRADATION (optional)
ABIOTSKA RAZGRADNJA (neobvezno)
% abiotic degradation = CO2
% abiotske razgradnje = CO2
A measured volume of inoculated mineral medium, containing a known concentration of test chemical (100 mg/litre of the test substance, to give at least 50-100 mg ThOD/litre) as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature (± 1o C or closer) for up to 28 days.
Izmerjeni volumen inokuliranega mineralnega medija, ki vsebuje znano koncentracijo preskusne snovi (100 mg preskusne snovi/l, da dobimo najmanj 50–100 mg TPK/l) kot edinega vira organskega ogljika, se do 28 dni meša v zaprti steklenici pri konstantni temperaturi (± 1 oC ali manj).
The consumption of oxygen is determined either by measuring the quantity of oxygen (produced electrolytically) required to maintain constant gas volume in the respirometer flask, or from the change in volume or pressure (or a combination of the two) in the apparatus.
Poraba kisika se določi bodisi z merjenjem količine kisika (elektrolitsko proizvedenega), potrebne za ohranjanje konstantnega volumna plina v respirometrski steklenici, bodisi iz spremembe volumna ali tlaka (ali kombinacije obeh) v aparaturi.
Evolved carbon dioxide is absorbed in a solution of potassium hydroxide or another suitable absorbent.
Razviti ogljikov dioksid se absorbira v raztopini kalijevega hidroksida ali drugega ustreznega absorbenta.
The amount of oxygen taken up by the test chemical (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD or COD.
Količina kisika, ki jo porabi preskusna snov (popravljena glede na sprejem pri slepem vzorcu z inokulumom, preskušenem vzporedno) se izrazi kot odstotek TPK ali KPK.
Optionally, primary biodegradation may also be calculated from supplemental specific analysis made at the beginning and end of incubation, and ultimate biodegradation by DOC analysis.
Po izbiri se lahko izračunata primarna biorazgradnja iz dopolnilne specifične analize, opravljene na začetku in na koncu inkubacije, ter popolna biorazgradnja z analizo DOC.
suitable respirometer;
ustrezni respirometer;
temperature control, maintaining ± 1 oC or better;
termostat, ki vzdržuje konstantno temperaturo do ± 1 oC ali bolj natančno;
membrane-filtration assembly (optional);
naprava za membransko filtracijo (neobvezno);
carbon analyser (optional).
analizator ogljika (neobvezno).
Mix 10 ml of solution (a) with 800 ml dilution water, add 1 ml of solutions (b) to (d) and make up to 1 litre with dilution water.
Zmešamo 10 ml raztopine (a) in 800 ml vode za redčenje, dodamo po 1 ml raztopin (b) do (d) in dopolnimo z vodo za redčenje do 1 litra.
The inoculum may be derived from a variety of sources: activated sludge; sewage effluents; surface waters and soils or from a mixture of these.
Inokulum se lahko pridobi iz različnih virov: aktivnega blata, komunalnih odplak, površinskih vod in prsti ali zmesi teh virov.
Prepare solutions of the test and reference chemicals, in separate batches, in mineral medium equivalent to a concentration, normally, of 100 mg chemical/litre (giving at least 50-100 mg ThOD/litre), using stock solutions.
Iz osnovnih raztopin pripravimo ločene serije raztopin preskusne in referenčne snovi v mineralnem mediju, ki ustrezajo koncentraciji 100 mg snovi/l (da dobimo najmanj 50–100 mg TPK/l).
Calculate the ThOD on the basis of formation of ammonium salts unless nitrification is anticipated, when the calculation should be based on nitrate formation (see Appendix 2.2)
TPK izračunamo na podlagi tvorbe amonijevih soli, razen če pričakujemo nitrifikacijo, takrat mora izračun temeljiti na tvorbi nitrata (glej Dodatek 2.2).
Determine the pH values and if necessary adjust to 7,4±0,2.
Določimo pH vrednost in jo po potrebi uravnamo na 7,4±0,2.
Poorly soluble substances should be added at a later stage (see below).
Slabo topne snovi se dodajo pozneje (glej spodaj).
If the toxicity of the test chemical is to be determined, prepare a further solution in mineral medium containing both test and reference chemicals at the same concentrations as in the individual solutions.
Če bomo določali strupenost preskusne snovi, pripravimo dodatno raztopino v mineralnem mediju, ki vsebuje preskusno in referenčno snov v istih koncentracijah, kakor posamezne raztopine.
If measurement of the physico-chemical uptake of oxygen is required, prepare a solution of the test chemical at, normally, 100 mg ThOD/litre which has been sterilised by the addition of a suitable toxic substance (see I.6.6).
Če potrebujemo meritev fizikalno-kemijskega sprejema kisika, pripravimo raztopino preskusne snovi, navadno v koncentraciji 100 mg TPK/l, ki jo steriliziramo z dodatkom ustrezne strupene snovi (glej I.6.6).
Introduce the requisite volume of solutions of test and reference chemicals, respectively, into at least duplicate flasks.
V steklenice, najmanj po dve za vsako vrsto vzorca, damo potrebne volumne raztopin preskusne in referenčne snovi.
Add to further flasks mineral medium only (for inoculum controls) and, if required, the mixed test/reference chemical solution and the sterile solution.
Pripravimo dodatne steklenice z le mineralnim medijem (za kontrolo inokuluma) ter, če je potrebno, z zmesjo raztopin preskusne in referenčne snovi in s sterilno raztopino.
If the test chemical is poorly soluble, add it directly at this stage on a weight or volume basis or handle it as described in Appendix 3.
Če je preskusna snov slabo topna, jo dodamo v tej fazi, neposredno v steklenico, na podlagi mase ali volumna, ali ravnamo z njo, kakor je opisano v Dodatku 3.
Add potassium hydroxide, soda lime pellets or other absorbent to the CO2-absorber compartments.
V razdelke CO2-absorberja damo kalijev hidroksid, tablete zmesi natrijevega ali kalijevega hidroksida in kalcijevega oksida ali kak drug absorbent.
preferably, and when necessary:
po možnosti in kadar je potrebno:
Flask 6: sterile control
Steklenica 6: Sterilni kontrolni vzorec
Flask 7: toxicity control
Steklenica 7: Vzorec za kontrolo strupenosti
Allow the vessels to reach the desired temperature and inoculate appropriate vessels with prepared activated sludge or other source of inoculum to give a concentration of suspended solids not greater than 30 mg/litre.
Počakamo, da steklenice dosežejo želeno temperaturo, in ustrezne steklenice inokuliramo s pripravljenim aktivnim blatom ali inokulumom iz drugega vira, tako da bo koncentracija neraztopljenih trdnih snovi največ 30 mg/l.
Assemble the equipment, start the stirrer and check for air-tightness, and start the measurement of oxygen uptake.
Sestavimo aparaturo, vklopimo mešalnik, preverimo nepredušnost in začnemo meriti sprejem kisika.
Usually no further attention is required other than taking the necessary readings and making daily checks to see that the correct temperature and adequate stirring are maintained.
Običajno nam ni treba storiti nič drugega, kot opraviti potrebne odčitke in dnevno preverjati, ali se vzdržujeta prava temperatura in ustrezno mešanje.
Calculate the oxygen uptake from the readings taken at regular and frequent intervals, using the methods given by the manufacturer of the equipment.
Sprejem kisika izračunamo iz vrednosti, odčitanih v rednih in pogostih intervalih po metodah, ki jih navaja proizvajalec opreme.
At the end of incubation, normally 28 days, measure the pH of the contents of the flasks, especially if oxygen uptakes are low or greater than ThODNH4 (for nitrogen-containing compounds).
Ob koncu inkubacije, navadno po 28 dneh, izmerimo pH vsebine steklenic, zlasti če je sprejem kisika manjši ali večji od ThODNH4 (za snovi, ki vsebujejo dušik).
If required, withdraw samples from the respirometer flasks, initially and finally, for analysis of DOC or specific chemical (see Appendix 2.4).
Če je potrebno, iz respirometrskih steklenic na začetku in na koncu odvzamemo vzorce za analizo DOC ali specifično kemijsko analizo (glej Dodatek 2.4).
At the initial withdrawal, ensure that the volume of test suspension remaining in the flask is known.
Ob prvem odvzemu zagotovimo, da je volumen preostale preskusne suspenzije v steklenici znan.
'When oxygen is taken up by N-containing test substance, determine the increase in concentration of nitrite and nitrate over 28 days and calculate the correction for the oxygen consumed by nitrification (Appendix 5).
Kadar kisik sprejema preskusna snov, ki vsebuje dušik, določimo povečanje koncentracije nitrita in nitrata v 28 dneh ter izračunamo popravek za kisik, porabljen pri nitrifikaciji (Dodatek 5).
Divide the oxygen uptake (mg) of the test chemical after a given time (corrected for that by the blank inoculum control after the same time) by the weight of the test chemical used.
Količino kisika (mg), ki ga sprejme preskusna snov po določenem času (popravljeno glede na sprejem v slepem vzorcu za kontrolo inokuluma v istem času), delimo z uporabljeno maso preskusne snovi.
This yields the BOD expressed as mg oxygen/mg test chemical, that is:
Tako dobimo BPK, izražen v mg kisika/mg preskusne snovi, to je:
BOD=mg O2
BPK=mg sprejetega O2
uptake by test chemical-mg O2
pri preskusni snovi – mg sprejetega O2
uptake by blank
pri slepem vzorcu
mg test chemical in flask
mg preskusne snovi v steklenici
= mg O2 per mg test chemical
= mg O2 na mg preskusne snovi
calculate the percentage biodegradation either from:
odstotek razgradnje izračunamo po enačbi:
% biodegradation = % ThOD =BODmg O2
% biorazgradnje = % TPK =BPKmg O2
/mg chemical
/mg snovi
chemical
snovi
or form
ali po enačbi
CODmg O2
KPKmg O2
It should be noted that these two methods do not necessarily give the same value; it is preferable to use the former method.
Opozoriti je treba, da ti dve metodi ne dasta nujno enakega rezultata; priporočena je uporaba prve metode.
For test substances containing nitrogen, use the appropriate ThOD (NH4 or NO3) according to what is known or expected about the occurrence of nitrification (Appendix 2.2).
Pri snoveh, ki vsebujejo dušik, uporabimo ustrezni TPK (NH4 ali NO3), glede na to kaj vemo ali pričakujemo glede nitrifikacije (Dodatek 2.2).
If nitrification occurs but is not complete, calculate a correction for the oxygen consumed by nitrification from the changes in concentration of nitrite and nitrate (Appendix 5).
Če pride do nitrifikacije, ki pa je nepopolna, izračunamo popravek za kisik, porabljen pri nitrifikaciji, iz sprememb v koncentraciji nitrita in nitrata (Dodatek 5).
When optional determinations of organic carbon and/or specific chemical are made, calculate the percentage degradation, as described under I.7.
Kadar določimo organski ogljik in/ali izvedemo specifično kemično analizo, odstotek razgradnje izračunamo, kakor je opisano v I.7.
Record all results on the data sheets attached.
Vse rezultate zapišemo na priložene podatkovne liste.
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/litre and should not be greater than 60 mg/litre in 28 days.
Sprejem kisika v slepem vzorcu z inokulumom je navadno 20–30 mg O2/l in ne sme biti večji od 60 mg/l v 28 dneh.
Values higher than 60 mg/litre require critical examination of the data and experimental techniques.
Pri vrednostih nad 60 mg/l je treba kritično preveriti podatke in tehniko preskušanja.
If the pH value is outside the range 6-8,5 and the oxygen consumption by the test chemical is less than 60% , the test should be repeated with a lower concentration of test chemical.
Če je pH vrednost izven območja 6–8,5 in poraba kisika pri preskusni snovi pod 60 %, je treba preskus ponoviti z manjšo koncentracijo preskusne snovi.
MANOMETRIC RESPIROMETRY TEST
PRESKUS MANOMETRIČNE RESPIROMETRIJE
Stock solution concentration: … mg/litre
Koncentracija osnovne raztopine: ... mg/liter
Initial concentration in medium, Co: … mg/litre
Začetna koncentracija v mediju, Co: ... mg/liter
Volume in test flask (V): … ml
Volumen v preskusni steklenici (V): ... ml
ThOD or COD: … mg O2/mg test substance (NH4 or NO3)
TPK ali KPK: ... mg O2/mg preskusne snovi (NH4 or NO3)
Concentration of suspended solids in reaction mixture: … mg/1
Koncentracija neraztopljenih trdnih snovi v reakcijski zmesi: ... mg/l
OXYGEN UPTAKE: BIODEGRADABILITY
SPREJEM KISIKA: BIORAZGRADLJIVOST
V = volume of medium in test flask
V = volumen medija v preskusni steklenici.
Time (Days)
Čas (dan)
upt. (mg) test chemical
(mg) preskusna snov
upt. (mg) blank
(mg) slepi vzorec
b, mean
b, srednja vrednost
Corrected BOD (mg)
Popravljeni spr. O2 (mg)
BOD per mg test chemical
BPK na mg preskusne snovi
% degradation
% razgradnje
Mean [5]
srednja vrednost [5]
N.B.: Similar formats may be used for the reference chemical and the toxicity controls.
Opomba: za referenčno snov in vzorce za kontrolo strapenosti se lahko uporabi podobna oblika.
CORRECTION FOR NITRIFICATION (see Annex V)
POPRAVEK ZA NITRIFIKACIJO (glej Prilogo V)
Concentration of nitrate (mg N/litre)
Koncentracija nitrata (mg N/l)
Oxygen equivalent (4,57 × N × V) (mg)
Ekvivalent kisika (4,57 × N × V) (mg)
Concentration of nitrite (mg N/litre)
Koncentracija nitrita (mg N/l)
Oxygen equivalent (3,43 × N × V) (mg)
Ekvivalent kisika (3,43 × N × V) (mg)
(ii + iv) Total oxygen equivalent
(ii + iv) Skupni ekvivalent kisika
Blank mg/litre
Slepi vzorec (mg/l)
Test chemical mg/litre
Preskusna snov mg/l
% DOC removed =1-Ct
% odstranjenega DOC =1-Ct
SPECIFIC CHEMICAL (optional)
SPECIFIČNA ANALIZA (neobvezno)
concentration in physico-chemical (sterile) control at 28 days
Koncentracija v fizikalno-kemijskem (sterilnem) kontrolnem vzorcu po 28 dneh
concentration in inoculated flask at 28 days,
Koncentracija v inokulirani steklenici po 28 dneh,
% biodegradation = Sb
% biorazgradnje = Sb
a oxygen consumption in sterile flasks after 28 days, (mg)
a poraba kisika v sterilnih steklenicah po 28 dneh,
oxygen consumption per mg test chemical = a×100
poraba kisika na mg preskusne snovi = a
(see Sections 1 and 3)
(glej oddelka 1 in 3)
% abiotic degradation = a×100
% abiotske razgradnje = a×100
The solution of the test chemical in mineral medium, usually at 2-5 mg/litre, is inoculated with a relatively small number of micro-organisms from a mixed population and kept in completely full, closed bottles in the dark at constant temperature.
Raztopina preskusne snovi v mineralnem mediju, običajno 2–5 mg/l, se inokulira z relativno majhnim številom mikroorganizmov iz mešane populacije in hrani v do vrha napolnjenih zaprtih steklenicah v temi pri konstantni temperaturi.
Degradation is followed by analysis of dissolved oxygen over a 28-day period.
Razgradnja se spremlja 28 dni prek analize raztopljenega kisika.
The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum run in parallel, is expressed as a percentage of ThOD or COD.
Količina kisika, ki jo porabi preskusna snov (popravljena glede na sprejem pri slepem vzorcu z inokulumom, preskušenem vzporedno), se izrazi kot odstotek TPK ali KPK.
BOD bottles, with glass stoppers, e.g. 250-300 ml;
BPK-steklenice s steklenimi zamaški, npr. 250–300 ml;
water bath or incubator, for keeping bottles at constant temperature (± 1 oC or better) with the exclusion of light;
vodna kopel ali inkubator, za hranjenje steklenic pri konstantni temperaturi (± 1 oC ali bolj natančno) in brez svetlobe;
large glass bottles (2-5 litres) for the preparation of media and for filling the BOD bottles;
velike steklenice (2–5 litrov) za pripravo medijev in polnjenje BPK-steklenic;
oxygen electrode and meter, or equipment and reagents for Winkler titration.
kisikova elektroda in merilnik ali oprema in reagenti za titracijo po Winklerju.
Mix 1 (one) ml of solution (a) to (d) and make up to 1 litre with dilution water.
Zmešamo po 1 ml raztopin (a) do (d) in dopolnimo do 1 litra z vodo za redčenje.
Preparation of the inoculum
Priprava inokuluma
The inoculum is normally derived from the secondary effluent of a treatment plant or laboratory-scale unit receiving predominantly domestic sewage.
Inokulum se navadno pridobi iz sekundarnega odtoka iz čistilne naprave ali laboratorijske enote, ki prejema predvsem gospodinjske odplake.
An alternative source for the inoculum is surface water.
Drugi vir inokuluma je površinska voda.
Normally use from one drop (0,05 ml) to 5 ml of filtrate per litre of medium; trials may be needed to discover the optimum volume for a given effluent (See I.6.4.2 and I.6.5).
Navadno uporabimo od ene kapljice (0,05 ml) do 5 ml filtrata na liter medija; morda bo treba s poskusi ugotoviti najprimernejši volumen za določen odtok (glej I.6.4.2 in I.6.5).
Strongly aerate mineral medium for at least 20 min. Carry out each test series with mineral medium derived from the same batch.
Mineralni medij močno prezračujemo najmanj 20 minut. Vsak niz preskusov izvedemo z mineralnim medijem iz iste serije.
Generally, the medium is ready for use after standing for 20 h, at the test temperature.
Na splošno je medij pripravljen za uporabo po 20 h pri temperaturi preskusa.
Determine the concentration of dissolved oxygen for control purposes; the value should be about 9 mg/litre at 20 oC.
Za namene kontrole določimo koncentracijo raztopljenega kisika; vrednost mora biti približno 9 mg/l pri 20 oC.
Conduct all transfer and filling operations of the air-saturated medium bubble-free, for example, by the use of siphons.
Vse postopke prenašanja in polnjenja z medijem, nasičenim z zrakom, opravimo tako, da v njem ne nastajajo mehurčki, na primer z uporabo sifonov.
Prepare parallel groups of BOD bottles for the determination of the test and reference chemicals in simultaneous experimental series.
Pripravimo vzporedne skupine BPK-steklenic za določanje preskusne in referenčne snovi v sočasnih preskusnih nizih.
Assemble a sufficient number of BOD bottles, including inoculum blanks, to allow at least duplicate measurements of oxygen consumption to be made at the desired test intervals, for example, after 0, 7, 14, 21 and 28 days.
Pripravimo zadostno število BPK-steklenic, vključno s slepimi vzorci z inokulumom, da omogočimo podvojene meritve porabe kisika v želenih časovnih intervalih, npr. po 0, 7, 14, 21 in 28 dneh.
To ensure being able to identify the 10-day window, more bottles may be required.
Lahko bo potrebnih več steklenic, da se omogoči določitev 10-dnevnega okna.
Add fully aerated mineral medium to large bottles so that they are about one-third full.
V velike steklenice damo popolnoma prezračen mineralni medij, tako da so napolnjene do približno ene tretjine.
Then add sufficient of the stock solutions of the test chemical and reference chemical to separate large bottles so that the final concentration of the chemicals is normally not greater than 10 mg/litre.
Nato v posamezne steklenice dodamo dovolj osnovne raztopine preskusne snovi ali referenčne snovi, da je končna koncentracija snovi največ 10 mg/l.
Add no chemicals to the blank control medium contained in a further large bottle.
V dodatno steklenico za slepi vzorec z medijem ne dodamo nobene snovi.
In order to ensure that the inoculum activity is not limited, the concentration of dissolved oxygen must not fall below 0,5 mg/litre in the BOD bottles.
Da je zagotovljeno, da aktivnost inokuluma ni omejena, koncentracija raztopljenega kisika v BPK-steklenicah ne sme pasti pod 0,5 mg/l.
This limits the concentration of test chemical to about 2 mg/litre.
To omejuje koncentracijo preskusne snovi na približno 2 mg/l. Pri slabo razgradljivih snoveh in tistih z majhnim TPK, lahko uporabimo tudi 5–10 mg/l.