natgillin/translations-raw huggingface dataset:
In that case, the temperature should vary by less than ± 5 °C in any one range of analyses.
V tem primeru mora biti obseg temperature manjši od ± 5 °C v kateri koli stopnji analize.
Samples must be taken in accordance with the procedure laid down in International Standard ISO 707.
Vzorci morajo biti vzeti v skladu z mednarodnim standardom ISO 707.
However, Member States may use another method of sampling provided that it complies with the principles of the abovementioned standard.
Vendar lahko države članice uporabijo drugo metodo vzorčenja, če je skladna z navedenim standardom.
Store the sample in conditions which preclude any deterioration or change in composition.
Vzorec hranite pri pogojih, ki izključujejo kakršno koli kvarjenje ali spremembo sestave.
Transfer the milk powder into a container with a capacity of about twice the volume of the powder, fitted with an airtight lid.
Prenesite mlečni prah v posodo s približno dvakrat večjo prostornino, kakor je prostornina prahu, ki ima nepredušen pokrov.
Close the container immediately.
Posodo takoj zaprite.
Mix the milk powder well by means of repeated inversion of the container.
Zmešajte mlečni prah s ponavljajočim obračanjem posode.
Test portion
Preskusni delež
Weight 2,000 ± 0,001 g of test sample into a centrifuge tube (6.2) or a suitable stoppered flask (50 ml).
Odtehtajte 2,000 ± 0,001 g preskusnega vzorca v epruveto za centrifugiranje (6.2) ali ustrezno zaprto bučko (50 ml).
Removal of fat and proteins
Odstranitev maščob in beljakovin
Add 20,0 ml of warm water (50 °C) to the test portion.
Odtehtanemu vzorcu dodajte 20,0 ml tople vode (50 °C).
Dissolve the powder by shaking for five minutes using a mechanical shaker (6.3).
Raztopite prah s petminutnim stresanjem na mehanskem stresalniku (6.3).
Place the tube into the water bath (6.10) and allow to equilibrate to 25 °C
Postavite epruveto v vodno kopel (6.10) in pustite, da se temperatura uravna na 25 °C.
Add 10,0 ml of the trichloroacetic acid solution (5.1) of ca. 25 °C in two minutes, while stirring vigorously with the aid of the magnetic stirrer (6.4).
V dveh minutah dodajte 10,0 ml raztopine trikloroocetne kisline (5.1) s temperaturo približno 25 °C in pri tem intenzivno mešajte z magnetnim mešalnikom (6.4).
Place the tube in a water bath (6.10) and leave for 60 minutes
Postavite epruveto v vodno kopel (6.10) in pustite 60 minut.
Centrifuge (6.2) for 10 minutes at 2200 g, or filter through paper (6.6), discarding the first 5 ml of filtrate
Centrifugirajte (6.2) 10 minut pri 2200 g, ali filtrirajte skozi filter (6.6) in zavrzite prvih 5 ml filtrata.
Inject 15 to 30 μl of accurately measured supernatant or filtrate (8.3.3) into the HPLC apparatus (6.11) operating at a flow rate of 1,0 ml of eluent solution (5.2) per minute
Vbrizgajte 15 do 30 μl natančno odmerjenega supernatanta ali filtrata (8.3.3) v HPLC-opremo (6.11), ki deluje s pretokom 1,0 ml raztopine eluenta (5.2) na minuto.
Note 1. Another flow rate may be used, dependent of the internal diameter of the columns used or the instructions of the manufacturer of the column.
Opomba 1: Uporabiti je mogoče tudi drugačen pretok, odvisno od notranjega premera kolon, ki so uporabljene, ali navodil proizvajalca kolon.
Note 2. Keep the eluent solution (5.2) at 85 °C throughout the chromatographic analysis in order to keep the eluent degassed and to prevent bacterial growth.
Opomba 2: Raztopina eluenta (5.2) naj ima med kromatografsko analizo 85 °C, kar omogoča vzdrževanje razplinjenja eluenta in preprečuje rast bakterij.
Never leave the eluent solution in them (5.2).
Nikoli ne pustite v njih raztopine eluenta (5.2).
Prior to any interruption of more than 24 hours, rinse the columns with water then wash them with solution (5.3) for at least three hours at a flow rate of 0,2 ml per minute.
Pred kakršno koli prekinitvijo, ki traja več kot 24 ur, spirajte kolone z vodo in nato z raztopino (5.3) pri pretoku 0,2 ml na minuto najmanj tri ure.
The results of chromatographic analysis of the test sample [E] are obtained in the form of chromatogram in which each peak is identified by its retention time RT as follows:
Rezultati kromatografske analize preskusnega vzorca [E] so dobljeni v obliki kromatogramov, pri katerih je vsak vrh opredeljen z retenzijskim časom RT, kakor sledi:
Peak II:
Vrh II:
The second peak of the chromatogram having an RT of about 12,5 minutes.
Drugi vrh kromatograma z retenzijskim časom približno 12,5 minute.
Peak III:
Vrh III:
The third peak of the chromatogram, corresponding to the CMP, having an RT of 15,5 minutes.
Tretji vrh kromatograma, ki ustreza CMP z retenzijskim časom približno 15,5 minute.
The choice of the column(s) can effect the retention times of the individual peaks considerably.
Izbira kolone ali kolon lahko bistveno vpliva na retenzijski čas posameznih vrhov.
The integrator (6.11.6) automatically calculates the area A of each peak:
Integrator (6.11.6) samodejno izračuna površino A vsakega vrha:
area of peak II,
površina vrha II,
area of peak III,
površina vrha III,
It is essential to examine the appearance of each chromatogram prior to quantitative interpretation, in order to detect any abnormalities due either to malfunctioning of the apparatus or the columns, or to the origin and nature of the sample analysed.
Pred kvantitativno interpretacijo je treba pregledati videz vsakega kromatograma, da bi tako lahko izsledili kakršne koli nepravilnosti kot posledice slabega delovanja bodisi kromatografa ali kolon ali pa kot posledico izvora in narave analiziranega vzorca.
If in doubt, repeat the analysis.
V primeru dvoma analizo ponovite.
Apply exactly the procedure described from point 8.2 to point 8.4.2 to the standard samples (5.4)
Za standardne vzorce (5.4) uporabite postopek, opisan od točke 8.2 do točke 8.4.2.
Use freshly prepared solutions, because CMP degrade in an 8 % trichloroacetic environment.
Uporabite sveže pripravljene raztopine, ker se CMP razgradi v 8-odstotnem trikloroocetnem okolju.
The loss is estimated at 0,2 % per hour at 30 °C.
Izguba je ocenjena na 0,2 % na uro pri 30 °C.
Prior to chromatographic determination of the samples, condition the columns by repeatedly injecting the standard sample (5.4.2) in solution (8.5.1) until the area and retention time of the peak corresponding to the CMP are constant
Pred kromatografskim določanjem vzorcev pripravite kolone s ponavljajočim vbrizgavanjem standardnega vzorca (5.4.2) v raztopino (8.5.1), dokler nista površina in retenzijski čas vrha, ki ustreza CMP, stalna.
Determine the response factors R by injecting the same volume of filtrates (8.5.1) as used for the samples
Z vbrizganjem enake prostornine filtrata (8.5.1), kakor je uporabljen pri vzorcih, določite odzivni faktor R.
Method of calculation and formulae
Metoda za izračun in enačbe
Calculation of the response factors R:
Izračun odzivnih faktorjev R:
the response factors of peaks II,
odzivni faktorji vrhov II,
the areas of peaks II of the standard sample [0] obtained in 8.5.3.
površine vrhov II standardnega vzorca [0], pridobljene v 8.5.3.
the response factor of peak III,
odzivni faktor vrha III,
A III [0] and AIII [5]
A III [0] in AIII [5]
the areas of peak III in standard samples [0] and [5] respectively obtained in 8.5.3,
površine vrha III standardnega vzorca [0] in [5], pridobljene v 8.5.3,
W the quantity of whey in standard sample [5], i.e. 5.
W količina sirotke v standardnem vzorcu [5], tj. 5.
Calculation of the relative area of the peaks in the sample [E]
Izračun relativnih površin vrhov vzorca [E]
the relative areas of peaks II, III and IV respectively in the sample [E],
relativne površine vrhov II, III in IV v vzorcu [E],
the areas of peaks II and III respectively in the sample [E] obtained in 8.4.2,
relativne površine vrhov II in III v vzorcu [E], pridobljene v 8.4.2,
the response factors calculated in 9.1.1.
odzivni faktorji, izračunani v 9.1.1.
Calculation of the relative retention time of peak III in sample [E]: RRTIII[E] = (RTIII[E])/(RTIII[5])
Izračun relativnega retenzijskega časa vrha III v vzorcu [E]: RRTIII[E] = (RTIII[E])/(RTIII[5])
the relative retention time of peak III in sample [E],
relativni retenzijski čas vrha III v vzorcu [E],
the retention time of peak III in sample [E] obtained in 8.4.2,
retenzijski čas vrha III v vzorcu [E], pridobljen v 8.4.2,
the retention time of peak III in control sample [5] obtained in 8.5.3.
retenzijski čas vrha III v kontrolnem vzorcu [5], pridobljen v 8.5.3,
Experiments have shown that there is a linear relation between the relative retention time of peak III, i.e. RRTIII [E] and the percentage of whey powder added up to 10 %
Preskusi so pokazali, da obstaja linearna povezava med relativnim retenzijskim časom vrha III, npr. RRTIII [E] in odstotkom dodane sirotke v prahu do 10 %
The RRTIII [E] is < 1,000 when the whey content is > 5 %;
RRTIII [E] je < 1,000, ko je vsebnost sirotke > 5 %,
the RRTIII [E] is ≥ 1,000 when the whey content is ≤ 5 %.
RRTIII [E] je ≥ 1,000, ko je vsebnost sirotke ≤ 5 %.
The uncertainty allowed for the values of RRTIII is ±0,002.
Dovoljena negotovost za vrednosti RRTIII je ±0,002.
Normally the value of RRTIII [0] deviates little from 1,034.
Običajno se vrednost RRTIII [0] giblje malo okoli 1,034.
Depending on the condition of the columns, the value may approach 1,000, but it must always be greater.
Odvisno od stanja kolon se vrednost lahko približa 1,000, ampak mora biti vedno večja od 1,000.
Calculation of the percentage of rennet whey powder in the sample:
Izračun vsebnosti siriščne sirotke v prahu v vzorcu:
W the percentage m/m of rennet whey in the sample [E];
W odstotek m/m siriščne sirotke v vzorcu [E];
the relative area of peak III of test sample [E] obtained as in 9.1.2;
relativna površina vrha III preskusnega vzorca [E] pridobljena v 9.1.2;
1,3 represents the relative average area of peak III expressed in grams of rennet whey per 100 g determined in non-adulterated skimmed-milk powder of various origins.
1,3 predstavlja relativno povprečno površino vrha III, izraženo v gramih siriščne sirotke na 100 g določene v posnetem mleku v prahu brez primesi različnega izvora.
This figure was obtained experimentally;
Ta podatek je bil pridobljen eksperimentalno;
represents the relative area of peak III which is equal to RIII × AIII [0].
predstavlja relativno površino vrha III, ki je enaka RIII × AIII [0].
These values are obtained in 9.1.1 and 8.5.3 respectively;
Te vrednosti so pridobljene v 9.1.1 in 8.5.3;
represents the correction to be made to the relative average area 1,3 when SIII [0] is not equal to 0,9.
predstavlja popravek za relativno povprečno površino 1,3, ko SIII [0] ni enako 0,9.
Experimentally the relative average area of peak III of the control sample [0] is 0,9.
Eksperimentalno je relativna povprečna površina vrha III kontrolnega vzorca [0] 0,9.
Accuracy of the procedure
Zanesljivost postopka
The difference between the results of two determinations carried out simultaneously or in rapid succession by the same analyst using the same apparatus on identical test material shall not exceed 0,2 % m/m.
Razlika med rezultatoma dveh določitev, ki ju izvede simultano ali po kratkem času isti analitik z isto opremo in na enakem preskusnem materialu, ne sme presegati 0,2 % m/m.
The difference between two single and independent results, obtained in two different laboratories on identical test material shall not exceed 0,4 % m/m.
Razlika med dvema posamičnima in neodvisnima rezultatoma, dobljenima v dveh različnih laboratorijih na enakem preskusnem materialu, ne sme presegati 0,4 % m/m.
Assume the absence of whey if the relative area of peak III, SIII [E] expressed in grams of rennet whey per 100 g of the product is ≤ 2,0 + (SIII[0] – 0,9) where
Če je relativna površina vrha III, SIII [E], izražena v gramih siriščne sirotke na 100 g proizvoda ≤ 2,0 + (SIII[0] – 0,9), je treba zanikati obstoj sirotke, kjer je
2,0 is the maximum value allowed for the relative area of peak III taking into account the relative area of peak III, i.e. 1,3, the uncertainty due to variations in the composition of skimmed-milk powder and the reproducibility of the method (9.3.2),
2,0 največja dovoljena vrednost relativne površine vrha III ob upoštevanju relativne površine vrha III, npr. 1,3, negotovost zaradi sprememb v sestavi posnetega mleka v prahu in obnovljivost metode (9.3.2),
is the correction to be made when the area SIII [0] is different from 0,9 (see point 9.2)
popravek, ki ga je treba upoštevati, ko se površina SIII [0] razlikuje od 0,9 (glej točko 9.2)
If the relative area of peak III, SIII [E] is > 2,0 + (SIII[0] – 0,9) and the relative area of peak II, SII [E] ≤ 160, determine the rennet whey content as indicated in point 9.2.
Če je relativna površina vrha III, SIII [E] > 2,0 + (SIII[0] – 0,9) in relativna površina vrha II, SII [E] ≤ 160, določite vsebnost siriščne sirotke, kot je navedeno v točki 9.2.
If the relative area of peak III, SIII [E] is > 2,0 + (SIII[0] – 0,9) and the relative area of peak II, SII [E] ≤ 160, determine the total protein content (P %); then examine graphs 1 and 2.
Če je relativna površina vrha III, SIII [E] > 2,0 + (SIII[0] – 0,9) in relativna površina vrha II, SII [E] ≤ 160, določite vsebnost skupnih beljakovin (P %); nato preučite diagrama 1 in 2.
The data obtained after analysis of samples of unadulterated skimmed-milk powders with a high total protein content have been assembled in graphs 1 and 2.
Podatki, dobljeni po analizi nepotvorjenih vzorcev posnetega mleka v prahu z visoko vsebnostjo skupnih beljakovin, so zbrani v grafih 1 in 2.
The continuous line represents the linear regression, the coefficients of which are calculated by the least squares method.
Neprekinjena črta predstavlja linearno regresijo, katere koeficienti so izračunani z metodo najmanjših kvadratov.
The dashed straight line fixes the upper limit of the relative area of peak III with a probability of not being exceeded in 90 % of cases.
Prekinjena ravna črta označuje zgornjo mejo relativne površine vrha III z verjetnostjo, da v 90 % primerov ni presežena.
The equations for the dashed straight lines of graphs 1 and 2 are:
Enačbi prekinjenih ravnih črt v grafih 1 in 2 sta:
(graph 1),
(graf 1),
(graph 2),
(graf 2),
respectively where:
kjer je:
the relative area of peak III calculated either according to total protein content or according to the relative area of peak SII [E],
relativna površina vrha III izračunana bodisi v skladu z vsebnostjo skupnih beljakovin bodisi v skladu z relativno površino vrha SII [E],
P % the total protein content expressed as a percentage, by weight,
P % vsebnost skupnih beljakovin, izražena v masnih odstotkih,
the relative area of sample calculated in point 9.1.2.
relativna površina vzorca, izračunana v točki 9.1.2.
These equations are equivalent to the figure of 1,3 mentioned in point 9.2.
Te enačbe so enakovredne vrednosti 1,3, navedeni v točki 9.2.
The discrepancy (T1 and T2) between the relative area SIII [E] found and the relative area SIII is given by means of the following: T1 = SIII[E] – [(0,376 P% – 10,7) + (SIII[0] – 0,9)]T2 = SIII[E] – [(0,0123 SII[E] + 0,93) + (SIII[0] – 0,9)]
Odstopanje (T1 in T2) med relativno površino SIII [E] in relativno površino SIII je prikazano z naslednjimi vrednostmi: T1 = SIII[E] – [(0,376 P% – 10,7) + (SIII[0] – 0,9)]T2 = SIII[E] – [(0,0123 SII[E] + 0,93) + (SIII[0] – 0,9)]
If T 1 and/or T2
Če je T 1 in/ali T2
are zero or less, the presence of rennet whey cannot be determined.
nič ali manj, prisotnosti siriščne sirotke ni mogoče določiti.
If T 1 and T2
Če sta T 1 in T2
exceed zero, rennet whey is present.
večji kot nič, je siriščna sirotka prisotna.
The rennet whey content is calculated according to the following formula: W = T2+0,91
Vsebnost siriščne sirotke se izračuna v skladu z naslednjo enačbo: W = T2+0,91
0,91 is the distance on the vertical axis between the continuous and dotted straight lines.
0,91 razdalja navpičnih osi med neprekinjenimi in pikčastimi ravnimi črtami.
Peak III
Vrh III
total protein content (%)
skupna vsebnost beljakovin (%)
Peak II
Vrh II
DETERMINING RENNET WHEY SOLIDS IN SKIMMED-MILK POWDER AND THE MIXTURES REFERRED TO IN REGULATION (EC) No 2799/1999
DOLOČANJE SUHIH SNOVI SIRIŠČNE SIROTKE V POSNETEM MLEKU V PRAHU IN MEŠANIC IZ UREDBE (ES) ŠT. 2799/1999
PURPOSE: DETECTING THE ADDITION OF RENNET WHEY SOLIDS TO:
NAMEN: UGOTAVLJANJE DODATKA SUHE SNOVI SIRIŠČNE SIROTKE K:
skimmed-milk powder as defined in Article 2 of Regulation (EC) No 2799/1999; and
posnetemu mleku v prahu, kakor je opredeljeno v členu 2 Uredbe (ES) št. 2799/1999, in
mixtures as defined in Article 4 of Regulation (EC) No 2799/1999.
mešanicam iz člena 4 Uredbe (ES) št. 2799/1999.
REFERENCES: INTERNATIONAL STANDARD ISO 707
SKLICI: MEDNARODNI STANDARD ISO 707
The content of rennet whey solids is defined as the percentage by mass as determined by caseinomacropeptide content by the procedure described.
Vsebnost trdnih snovi siriščne sirotke je opredeljena kot masni odstotek, ki se določi z vsebnostjo makropeptidov kazeina v skladu z opisanim postopkom.
Caseinomacropeptides content is determined in accordance with Annex XII.
Vsebnost makropeptidov kazeina se določi skladno s Prilogo XII.
Samples giving positive results are analysed for caseinomacropeptide A by a reversed-phase high-performance liquid chromatography procedure (HPLC procedure).
Pri vzorcih, ki dajejo pozitivne rezultate, se analizira vsebnost makropeptida kazeina A s tekočinsko kromatografijo visoke ločljivosti na reverzni fazi (HPLC-postopek).
Alternatively samples are directly analysed by the reversed-phase HPLC procedure.
Druga možnost je, da se vzorci neposredno analizirajo z reverzno fazo HPLC-postopka.
Evaluation of the result is obtained by reference to standard samples consisting of skimmed-milk powder with and without a known percentage of whey powder.
Rezultati se vrednotijo glede na standardne vzorce posnetega mleka v prahu z znanim dodatkom in brez znanega dodatka sirotke v prahu.
Results higher than 1 % (m/m) show that rennet whey solids are present.
Rezultati, ki presegajo 1 % masnega deleža, kažejo na prisotnost trdnih snovi siriščne sirotke.
Acetonitrile should be of spectroscopic or HPLC quality.
Kakovost acetonitrila mora biti primerna za spektroskop ali HPLC.
Reagents needed for the procedure are described in Annex XII to this Regulation.
Reagenti, ki so potrebni za postopek, so opisani v Prilogi XII k tej uredbi.
Reagents for reversed phase HPLC.
Reagenti za HPLC na reverzni fazi.
Eluents A and B
Eluenta A in B
Conservation of the column
Shranitev kolone
After the analyses the column is flushed with eluent B (via a gradient) and subsequently flushed with acetonitrile (via a gradient for 30 minutes).
Po analizi kolono sperete z eluentom B (gradientno) in potem še z acetonitrilom (gradientno v 30 minutah.).
The column is stored in acetonitrile.
Kolona se shrani v acetonitrilu.
Skimmed-milk powder meeting the requirements for public storage (i e. [0]).
Posneto mleko v prahu, ki ustreza zahtevam za javno skladiščenje (npr. [0])
The same skimmed-milk powder adulterated with 50 % (m/m) rennet-type whey powder of standard composition (i.e. [50]) [1].
Enako posneto mleko v prahu z dodatkom 50 % (m/m) siriščne sirotke v prahu s standardno sestavo (tj. [50]) [1].
Apparatus needed for the procedure described is described in Annex XII to this Regulation.
Oprema, ki je potrebna za omenjeni postopek, je opisana v Prilogi XII k tej uredbi.
Graduated pipettes, allowing delivery of 10 ml (ISO 648, Class A, or ISO/R 835), or a dispensing system capable of delivering 10,0 ml in two minutes
Graduirana pipeta, ki prepušča 10 ml (ISO 648, razred A, ali ISO/R 835), ali razdelilni sistem, ki lahko prepušča 10,0 ml v dveh minutah
Binary gradient pumping system
Binarni gradientni sistem črpalk
Injector, hand or automatic, with a 100 μl capacity
Injektor, ročni ali avtomatski, s prostornino 100 μl
Agilent Technologies Zorbax 300 SB-C3 column (length 25 cm, 0,46 cm internal diameter) or an equivalent wide-pore silica based reversed-phase column
Kolona Agilent Technologies Zorbax 300 SB-C3 (dolžina 25 cm, notranji premer 0,46 cm) ali enakovredna silicijeva kolona s širokimi porami za reverzno kromatografijo
Variable wavelength UV detector, permitting measurements at 210 nm (if necessary, a higher wavelength up to 220 nm may be used) with a sensitivity of 0,02 Å
UV-detektor z nastavljivo valovno dolžino in z možnostjo merjenja pri 210 nm (če je potrebno, se lahko uporabi višja valovna dolžina do 220 nm) z občutljivostjo 0,02 Å.
Integrator capable of setting the integration to common baseline or valley-to-valley
Integrator, ki lahko določi integracijo na splošni bazni liniji ali dolina–dolina
Note: Operation of the column at room temperature is possible, provided that the room temperature does not fluctuate more than 1 °C, otherwise too much variation in the retention time of CMPA takes place.
Opomba: Delo s kolonami je mogoče tudi pri sobni temperaturi, če temperatura niha manj kot ± 1 °C, sicer nastane preveliko nihanje retenzijskega časa CMPA.
However, Member States may use another method of sampling provided that it complies with the principles of the abovementioned standard
Vendar lahko države članice uporabijo drugo metodo vzorčenja, če je skladna z navedenim standardom.
Mix the milk powder well by means of repeated inversion of the container
Zmešajte mlečni prah s ponavljajočim obračanjem posode.
Weigh 2,00 ± 0,001 g of test sample into a centrifuge tube (6.2) or suitable stoppered flask (50 ml).
Odtehtajte 2,00 ± 0,001 g preskusnega vzorca v epruveto za centrifugiranje (6.2) ali ustrezno zaprto bučko (50 ml).
Note: In the case of mixtures, weigh such an amount of the test sample that the defatted sample portion corresponds to 2,00 g.
Opomba: V primeru mešanic odtehtajte takšno količino preskusnega vzorca, da razmaščeni vzorec deleža ustreza 2,00 g.
Dissolve the powder by shaking for five minutes or 30 minutes in the case of acid buttermilk using a mechanical shaker (6.3).
Raztopite prah s petminutnim stresanjem na mehanskem stresalniku ali tridesetminutnim stresanjem v primeru kislega pinjenca (6.3).
Add 10,0 ml of the trichloroacetic acid solution of ca. 25 °C (5.1) constantly over two minutes, while stirring vigorously with the aid of the magnetic stirrer (6.4).
V dveh minutah dodajte 10,0 ml raztopine triklorocetne kisline s temperaturo približno 25 °C (5.1) in pri tem intenzivno mešajte z magnetnim mešalnikom (6.4).
Centrifuge (6.2) 2200 g for 10 minutes, or filter through paper (6.6), discarding the first 5 ml of filtrate
Centrifugirajte (6.2) 10 minut pri 2200 g ali filtrirajte skozi filter in zavrzite prvih 5 ml filtrata.
Perform HPLC-analysis as described in Annex XII.
Izvedite HPLC-analizo, kakor je opisano v Prilogi XII.
If a negative result is obtained, the sample analysed does not contain rennet-whey solids in detectable amounts.
Če je dobljeni rezultat negativen, analizirani vzorec ne vsebuje suhe snovi siriščne sirotke v zaznavnih količinah.
If the result is positive, the reversed-phase HPLC procedure described below must be applied.
Če je rezultat pozitiven, je treba uporabiti HPLC-kromatografijo na reverzni fazi.
Alternatively the reversed-phase HPLC method can directly be applied.
V nasprotnem primeru se lahko neposredno uporabi metoda HPLC na reverzni fazi.
The presence of acid buttermilk powder may give rise to false-positive results using the method described in Annex XII.
Prisotnost kislega pinjenca lahko privede do lažno pozitivnih rezultatov z uporabo metode iz Priloge XII.
The reversed-phase HPLC method excludes this possibility
Metoda HPLC na reverzni fazi to možnost izključuje.
Before the reversed phase HPLC-analysis is carried out, the gradient conditions should be optimised.
Pred izvedbo HPLC-analize na reverzni fazi je treba optimizirati gradientne pogoje.
A retention time of 26 ± 2 minutes for CMPA is optimal for gradient systems having a dead volume of about 6 ml (volume from the point where the solvents come together to the volume of the injector loop, inclusive).
Retenzijski čas CMPA 26 ± 2 minuti je optimalen za gradientne sisteme z mrtvo prostornino približno 6 ml (prostornina od točke, na kateri pridejo skupaj topila, do vključno s prostornino injekcijske zanke).