natgillin/translations-raw huggingface dataset:
the nominal test concentrations, the means of the measured values and their standard deviations in the test vessels and the method by which these were attained,
nominalne preskusne koncentracije, povprečje izmerjenih vrednosti in njihovih normalnih odklonov v preskusnih posodah ter metoda, s pomočjo katere so bili izmerjeni,
source of the dilution water, description of any pre-treatment, results of any demonstration of the ability of test fish to live in the water, and water characteristics: pH, hardness, temperature, dissolved oxygen concentration, residual chlorine levels (if measured), total organic carbon, suspended solids, salinity of the test medium (if appropriate) and any other measurements made,
vir vode za pripravo raztopine, opis kakršnih koli predobdelav, rezultati katerih koli predstavitev sposobnosti preskusnih rib, da živijo v tej vodi, in značilnosti vode: pH, trdota, temperatura, koncentracija raztopljenega kisika, stopnje ostanka klora (če so izmerjene), skupni organski ogljik, suspendirane suhe snovi, slanost preskusnega gojišča (če je to primerno) in katere koli druge opravljene meritve,
water quality within test vessels, pH, hardness, TOC, temperature and dissolved oxygen concentration,
kakovost vode v preskusnih posodah, pH, trdota, TOC, temperatura in koncentracija raztopljenega kisika,
detailed information on feeding (e.g. type of food, source, composition — at least lipid and protein content if possible, amount given and frequency),
podrobni podatki o hranjenju (na primer vrsta hrane, vir, sestava – najmanj vsebnost lipida in proteina, če je možno, količina dane hrane in pogostost),
information on the treatment of fish and water samples, including details of preparation, storage, extraction and analytical procedures (and precision) for the test substance and lipid content (if measured).
podatki o ravnanju z ribjimi in vodnimi vzorci, vključno s podrobnostmi o pripravi, shranjevanju, ekstrakciji in analitičnih postopkih (in natančnost) za preskusno snov in vsebnost lipida (če je izmerjena).
RESULTS:
REZULTATI:
results from any preliminary study performed,
rezultati vseh poprej izvedenih študij,
mortality of the control fish and the fish in each exposure chamber and any observed abnormal behaviour,
smrtnost kontrolnih rib in rib v vsaki posodi s preskusno snovjo ter kakršno koli zabeleženo nenavadno obnašanje,
the lipid content of the fish (if determination on testing occasion),
vsebnost lipida v ribah (če je določena med preskusom),
curves (including all measured data) showing the uptake and depuration of the test chemical in the fish, the time to steady-state,
krivulje (vključno z vsemi izmerjenimi podatki) iz katerih je razvidna absorpcija in očiščenje preskusne kemikalije v ribi ter čas, potreben za dosego stabilnega stanja,
the steady-state bioconcentration factor (BCFss) and/or kinetic concentration factor (BCFK) and if applicable, 95 % confidence limits for the uptake and depuration (loss) rate constants (all expressed in relation to the whole body and the total lipid content, if measured, of the animal or specified tissues thereof), confidence limits and standard deviation (as available) and methods of computation/data analysis for each concentration of test substance used,
biokoncentracijski faktor stabilnega stanja (BKFSS) in/ali kinetični koncentracijski faktor (BKFk) ter, če je to primerno, 95 % meje zaupanja za konstante stopenj absorpcije in očiščenja (izgube) (vse izraženo v odnosu do celega telesa in skupne vsebnosti lipida v živali ali določenih tkiv le-te, če je izmerjena), meje zaupanja in standardna deviacija (če je na razpolago) in metode analize računavanja/analize podatkov za vsako koncentracijo uporabljene preskusne snovi,
where radio-labelled substances are used, and if it is required, the accumulation of any detected metabolites may be presented,
kadar so uporabljene radioaktivno označene snovi, in če je to potrebno, naj se predloži kopičenje vseh zaznanih metabolitov,
anything unusual about the test, any deviation from these procedures, and any other relevant information,
kar koli nenavadnega v zvezi s preskusom, kakršno koli odstopanje od teh postopkov in kateri koli drugi pomembni podatki,
minimise results as ‘not detected at the limit of detection’ by pre-test method development and experimental design, since such results cannot be used for rate constant calculations.
z razvojem metode pred preskusom in s preskusnim načrtom je treba zmanjšati rezultate v smislu „neopaženo znotraj mej zaznavnosti“, saj se takšni rezultati ne morejo uporabiti za izračun konstante.
SAR and QSAR in Environmental Research, 1, p. 29-390.
SAR and QSAR in Environmental Research, 1, str. 29–390.
OECD, Paris (1996) Direct Phototransformation of chemicals in water.
OECD, Pariz (1996). Direct phototransformation of chemicals in water.
Pesticide analytical manual, 1, 5600 Fisher's Lane, Rockville, MD 20852, July 1975.
Pesticide analytical manual, 1, 5600 Fisher's Lane, Rockville, MD 20852, Juli 1975.
Authors: P. Kristensen and N. Nyholm.
Avtorja: P. Kristensen in N. Nyholm.
Chemical characteristics of an acceptable dilution water
Kemične lastnosti sprejemljive razredčene vode
Limit concentration
MEJNA KONCENTRACIJA
Particulate Matter
Delci
Total Organic Carbon
Skupni organski ogljik
Un-ionised ammonia
Neionizirani amoniak
Total organophosphorous pesticides
Skupni organofosforni pesticidi
Total organochlorine pesticides plus polychlorinated biphenyls
Skupni organoklorni pesticidi in poliklorirani bifenili
Danio rerio [1] (Teleostei, Cyprinidae) (Hamilton-Buchanan) Zebra — fish
Danio rerio [1] (Teleostei, Cyprinidae) (Hamilton-Buchanan) navadna cebrica
Cyprinus carpio (Teleostei, Cyprinidae) (Linnaeus) Common Carp
Cyprinus carpio (Teleostei, Cyprinidae) (Linnaeus) Navadni krap
Oryzias latipes (Teleostei, Poeciliidae) (Temminck and Schlegel) Ricefish
Oryzias latipes (Teleostei, Poeciliidae) (Temminck and Schlegel) medaka
Poecilia reticulata (Teleostei, Poeciliidae) (Peters) Guppy
Poecilia reticulata (Teleostei, Poeciliidae) (Peters) gupi
Oncorhynchus mykiss (Teleostei, Salmonidae) (Walbaum) Rainbow trout
Oncorhynchus mykiss (Teleostei, Salmonidae) (Walbaum) šarenka
Gasterosteus aculeatus (Teleostei, Gasterosteidae) (Linnaeus) Three-spined stickleback
Gasterosteus aculeatus (Teleostei, Gasterosteidae) (Linnaeus) zet
Various estuarine and marine species have been used in different countries, for example:
Različne estuarske in morske vrste se uporabljajo v različnih državah, na primer:
Sheepshead minnow
biserjasti krapič
Shiner perch
svetlikavi ostriž
English sole
angleška iverka
Three-spined stikleback
Leptocottus armatus zet
Bleak
zelenika
The fresh water fish listed in the table above are easy to rear and/or are widely available throughout the year, whereas the availability of marine and estuarine species is partially confined to the respective countries.
Sladkovodne ribe, navedene v tabeli, se gojijo brez posebnosti in/ali so zlahka na voljo vse leto, razpoložljivost morskih in estuarskih vrst pa je deloma omejena na posamezne države.
They are capable of being bred and cultivated either in fish farms or in the laboratory, under disease-and parasite-controlled conditions, so that the test animal will be healthy and of known parentage.
Vzgojijo in gojijo se lahko ali v ribogojnicah ali laboratoriju, pod pogoji, ki nadzirajo bolezni in parazite, tako da je preskusna žival zdrava in znanega porekla.
Meyer A., Orti G. (1993) Proc.
Meyer A., Orti G. (1993) Proc. Royal Society of London, Series B., zv.
Royal Society of London, Series B., Vol. 252, p. 231.
252, str. 231
Prediction of the duration of the uptake and depuration phases
Napoved trajanja faz absorpcije in očiščenja
Prediction of the duration of the uptake phase
Napoved trajanje faze absorpcije
Before performing the test, an estimate of k2 and hence some percentage of the time needed to reach steady-state may be obtained from empirical relationships between k2 and the n-octanol/water partition coefficient (Pow) or k2 and the aqueous solubility (s).
Pred izvedbo preskusa se lahko ocena k2 in posledično nekaj odstotkov časa, potrebnega za dosego stabilnega stanja, pridobi na osnovi empiričnih razmerij med k2 in koeficientom porazdelitve n-oktanol/voda (Pow) ali k2 in topnostjo v vodi (s).
An estimate of k2 (day-1) may be obtained, for example from the following empirical relationship (1):
Ocena k2 (dan-1) se lahko pridobi, na primer, na podlagi naslednjega empiričnega razmerja (1):
= 0,414 log10(Pow) + 1,47 (r2 = 0,95)
= 0,414 log10(Pow) + 1,47(r2 = 0,95)
[equation 1]
[enačba 1]
For other relationships see Ref. (2).
Za druga razmerja glej literaturo (2).
If the partition coefficient (Pow) is not known, an estimate can be made (3) from a knowledge of the aqueous solubility (s) of the substance using:
Če koeficient porazdelitve (Pow) ni znan, se ocena lahko pridobi (3) na podlagi poznavanja topnosti snovi v vodi (s):
[equation 2]
[enačba 2]
s = solubility (moles/l) : (n=36)
s = topnost (mol/l) : (n=36)
These relationships apply only to chemicals with log Pow values between 2 and 6,5 (4).
Ta razmerja veljajo samo za kemikalije z log Pow vrednostmi med 2 in 6,5 (4).
The time to reach some percentage of steady-state may be obtained, by applying the k2-estimate, from the general kinetic equation describing uptake and depuration (first-order kinetics):
Čas, potreben za dosego določenega odstotka stabilnega stanja, se lahko izračuna s pomočjo ocene k2 na podlagi splošne kinetične enačbe, ki opisuje absorpcijo in očiščenje (kinetika prvega reda):
or if Cw is constant:
ali če je Cw konstanta:
[equation 3]
[enačba 3]
When steady-state is approached (t → ∞), equation 3 may be reduced (5) (6) to:
Kadar se približamo stabilnemu stanju (t→∞), se enačba 3 lahko okrajša (5) (6):
Then k1/k2 · Cw is an approach to the concentration in the fish at ‘steady-state’ (Cf,s).
Potem je k1/k2 .Cw približek koncentraciji v ribi pri „stabilnem stanju“ (Cf, s).
Equation 3 may be transcribed to:
Enačba 3 se lahko zapiše kot:
or Cf
aliCf
[equation 4]
[enačba 4]
Applying equation 4, the time to reach some percentage of steady-state may be predicted when k2 is pre-estimated using equation 1 or 2.
Z uporabo enačbe 4 se čas za dosego določenega odstotka stabilnega stanja lahko predvidi, kadar je k2 predhodno ocenjen z uporabo enačb 1 ali 2.
As a guideline, the statistically optimal duration of the uptake phase for the production of statistically acceptable data (BCFK) is that period which is required for the curve of the logarithm of the concentration of the test substance in fish plotted against linear time to reach its mid-point, or 1,6/k2, or 80 % of steady-state but not more than 3,0/k2 or 95 % of steady-state (7).
Kot napotek: statistično najugodnejše trajanje faze absorpcije za pridobitev statistično sprejemljivih podatkov (BKFk) je tisto obdobje, ki se zahteva za krivuljo logaritma koncentracije preskusne snovi v ribah, načrtano proti linearnemu času, da doseže razpolovišče, ali 1,6/k2, ali 80 % stabilnega stanja, ne pa več kot 3,0/k2 ali 95 % stabilnega stanja (7).
The time to reach 80 % of steady-state is (equation 4):
Čas, potreben za dosego 80 % stabilnega stanja je (enačba 4):
[equation 5]
[enačba 5]
Similarly 95 percent of steady-state is:
Podobno je 95 % stabilnega stanja:
[equation 6]
[enačba 6]
For example, the duration of the uptake phase (up) for a test substance with log Pow = 4 would be (using equations 1,5,6):
Na primer, trajanje faze absorpcije (up) za preskusno snov z log Pow = 4 bi bilo (ob uporabi enačb 1, 5 in 6):
= 0,652 days-1
= 0,652 dni-1
up (80 %) = 1,6/0,652, i.e. 2,45 days (59 hours)
fa (80 %) = 1,6/0,652, t.y. tj. 2,45 dni (59 ur)
or up (95 %) = 3,0/0,652, i.e. 4,60 days (110 hours)
ali fa (95 %) = 3,0/0,652, t.y. tj. 4,60 dni (110 ur)
Similarly, for a test substance with s = 10-5 mol/l (log(s) = -5,0), the duration of up would be (using equations 1,2,5,6):
Prav tako bi faza absorpcije za preskusno snov, ki ima s = 10-5 mol/l (log(s) = -5,0), trajala (ob uporabi enačb 1, 2, 5 in 6):
= 0,246 days -1
= 0,246 dni -1
up (80 %) = 1,6/0,246, i.e. 6,5 days (156 hours)
fa (80 %) = 1,6/0,246, tj. 6,5 dni (156 ur)
or up (95 %) = 3,0/0,246, i.e. 12,2 days (293 hours)
ali fa (95 %) = 3,0/0,246, tj. 12,2 dni (293 ur)
Alternatively, the expression:
Uporabi se lahko tudi izraz:
teq = 6,54 × 103 Pow + 55,31 (hours)
teq = 6,54 × 103 Pow + 55,31 (ur)
may be used to calculate the time for effective steady-state to be reached (4).
za izračun časa, potrebnega za dosego efektivnega stabilnega stanja (4).
Prediction of the duration of the depuration phase
Napoved trajanja faze očiščenja
A prediction of the time needed to reduce the body burden to some percentage of the initial concentration may also be obtained from the general equation describing uptake and depuration (first order kinetics) (1) (8).
Napoved časa, potrebnega za zmanjšanje obremenitve telesna na nekaj odstotkov začetne koncentracije, se lahko prav tako izračuna iz splošne enačbe, ki opisuje absorpcijo in očiščenje (kinetika prvega reda) (1) (8).
For the depuration phase, Cw is assumed to be zero.
Za fazo očiščenja se predvideva, da je Cw enak nič.
The equation may be reduced to:
Enačba se lahko okrajša na:
where Cf, o is the concentration at the start of the depuration period.
kjer je Cf,0 koncentracija na začetku faze očiščenja.
50 % depuration will then be reached at the time (t50):
50 % očiščenja bo tako opravljenega v času (t50):
or t50
alit50
Similarly 95 % depuration will be reached at:
Podobno se bo 95 % očiščenja doseglo pri:
If 80 % uptake is used for the first period (1,6/k2) and 95 % loss in the depuration phase (3,0/k2), then the depuration phase is approximately twice the duration of the uptake phase.
Če se 80 % absorpcije uporabi za prvo obdobje 1,6/k2) in 95 % izgube v fazi očiščenja (3,0/k2), potem je faza očiščenja približno dvakrat daljša od faze absorpcije.
It is important to note, however, that the estimations are based on the assumption that uptake and depuration patterns will follow first order kinetics.
Pomembno pa je opozoriti, da ocene temeljijo na predvidevanju, da fazi absorpcije in očiščenja sledita kinetiki prvega reda.
If first order kinetics are obviously not obeyed, more complex models should be employed (e.g. ref (1)).
Če kinetika prvega reda očitno ne ustreza, je treba uporabiti bolj zapletene modele (npr. literatura (1)).
Literature (of Appendix 3)
Literatura (Dodatek 3)
Res. 22 (6), pp 701-707.
22 (6), str. 701–707.
Transactions of the American Fisheries Society, 104 (4), pp 785-792.
Transactions of the American Fisheries Society, 104 (4). str. 785–792.
Part 4.4 pp 243-255.
Part 4.4 str. 243–255.
Könemann H. and Van Leeuwen K. (1980) Toxicokinetics in fish: Accumulation and Elimination of six Chlorobenzenes by Guppies.
Konemann H. and Van Leeuwen K. (1980) Toxicokinetics in fish: Accumulation and Elimination of six Chlorobenzenes by Guppies.
Chemosphere, 9, pp 3-19.
Chemosphere, 9, str. 3–19.
Theoretical example of sampling schedule for bioconcentration tests of substances with log Pow = 4
Teoretični primer vzorčne razporeditve biokoncentracijskih preskusov snovi z log Pow = 4
Values in brackets are numbers of samples (water, fish) to be taken if additional sampling is carried out.
Vrednosti v oklepajih so števila vzorcev (vode, rib), ki naj se upoštevajo, če se izvede dodatno vzorčenje.
pre-test estimate of k2 for log Pow of 4,0 is 0,652 days-1.
Predhodna ocena k2 za log Pow = 4,0 je 0,652 dni-1.
The total duration of the experiment is set to 3 × up = 3 × 4,6 days, i.e. 14 days.
Skupno trajanje preskusa znaša 3 × up = 3 × 4,6 dni, t.j. 14 dni.
For the estimation of ‘up’ refer to Appendix 3.
Za oceno „up“ glej Dodatek 3.
Fish sampling
Vzročenje rib
Sample time schedule
Časovni razpored vzorčenja
No of water samples
Število vodnih vzorcev
No of fish per sample
Število rib na vzorec
Minimal required frequency (days)
Minimalna zahtevana pogostnost (v dnevih)
Additional sampling
Dodatno vzorčenje
Uptake phase
Faza absorpcije
add 45-80 fish
dodati 45–80 rib
Sample water after minimum of 3 ‘chamber-volume’ have been delivered.
Vzorčna voda po najmanj treh zamenjanih vsebinah vode.
Model discrimination
Razlikovanje modelov
Most bioconcentration data have been assumed to be ‘reasonably’ well described by a simple two-compartment/two-parameter model, as indicated by the rectilinear curve which approximates to the points for concentrations in fish, during the depuration phase, when these are plotted on semi-log paper.
Za večino biokoncentracijskih podatkov se predvideva, da se „razmeroma“ dobro opišejo s preprostim dvodelnim/dvoparametrskim modelom, kot je nakazano s premočrtno krivuljo, ki se približuje točkam za koncentracijo v ribah med fazo očiščenja, kadar so le-te grafično prikazane na pollogaritemskem papirju.
(Where these points cannot be described by a rectilinear curve then more complex models should be employed, see, for example, Spacie and Hamelink, Ref 1 in Appendix 3).
(Kadar te točke ne morejo biti načrtane s premočrtno krivuljo, je treba uporabiti bolj zapletene modele, glej na primer Spacie in Hamelink, literatura 1 v Dodatku 3).
Graphical method for determination of depuration (loss) rate constant k2
Grafična metoda za določanje konstante stopnje očiščenja (izgube) k2
Plot the concentration of the test substance found in each sample of fish against sampling time on semi-log paper.
Koncentracija preskusne snovi, ki se nahaja v vsakem vzorcu rib, naj se načrta proti času vzorčenja na pollogaritemskem papirju.
The slope of that line is k2.
Nagib te linije je k2.
Note that deviations from straight line may indicate a more complex depuration pattern than first order kinetics.
Odstopanje od ravne linije lahko kaže na bolj zapleten vzorec očiščenja kot je kinetika prvega reda.
A graphical method may be applied for resolving types of depuration deviating from first order kinetics.
Grafična metoda se lahko uporabi za ugotavljanje tipov očiščenja, ki odstopajo od kinetike prvega reda.
Graphical method for determination of uptake rate constant k1
Grafična metoda za določanje konstante stopnje absorpcije k1
Given K2, calculate k1 as follows:
Če je k2 podan, se k1 izračuna po naslednji enačbi:
The value of Cf is read from the midpoint of the smooth uptake curve produced by the data when log concentration is plotted versus time (on an arithmetical scale).
Vrednost Cf se razbere iz razpolovišča enakomerno padajoče krivulje absorpcije na podlagi podatkov, ko je logaritemska koncentracija načrtana proti času (na aritmetični lestvici).
Computer method for calculation of uptake and depuration (loss) rate constants
Računalniška metoda za izračun konstant absorpcije in očiščenja (izgube)
The preferred means for obtaining the bioconcentration factor and k1 and k2 rate constants is to use non-linear parameter estimation methods on a computer.
Zaželeno je, da se za izračun biokoncentracij skega faktorja ter konstant stopenj k1 in k2 uporabijo računalniške nelinearne parametrične metode ocenjevanja.
These programs find values for k1 and k2 given a set of sequential time concentration data and the model:
Ti programi najdejo vrednosti za k1 in k2, če imajo dovolj podatkov o koncentraciji zaporednega časa in model:
where tc = time at the end of the uptake phase.
kjer je tc = čas na koncu faze absorpcije.
This approach provides standard deviation estimates of k1 and k2.
Ta pristop omogoča ocene standardne deviacije k1 in k2.
As k2 in most cases can be estimated from the depuration curve with relatively high precision, and because a strong correlation exists between the two parameters k1 and k2 if estimated simultaneously, it may be advisable first to calculate k2 from the depuration data only, and subsequently calculate k1 from the uptake data using non-linear regression.
Ker se k2 v večini primerov lahko oceni na podlagi krivulje očiščenja z relativno visoko natančnostjo in ker med parametroma k1 in k2 obstaja močna soodvisnost, če ju določamo sočasno, je priporočljivo, da se najprej izračuna k2 zgolj na podlagi podatkov očiščenja in šele nato k1 na osnovi podatkov absorpcije, pri čemer se uporabi nelinearna regresija.
This growth toxicity test method is a replicate of the OECD TG 215 (2000).
Ta metoda preskušanja toksičnosti za rast ustreza metodi OECD TG 215 (2000).
This test is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish.
Ta preskus je namenjen ocenjevanju vplivov daljše izpostavljenosti kemikalijam na rast ribjih mladic.
It is based on a method, developed and ring-tested (1)(2) within the European Union, for assessing the effects of chemicals on the growth of juvenile rainbow trout (Oncorynchus mykiss) under flow-through conditions.
Temelji na metodi, ki je bila razvita in krožno preskušena (1) (2) v Evropski uniji, za ocenjevanje vplivov kemikalij na rast mladic šarenke (Oncorhynchus mykiss) v pretočnih pogojih.
Other well documented species may be used.
Lahko se uporabljajo tudi druge dobro dokumentirane vrste.
For example, experience has been gained from growth tests with zebrafish (Danio rerio) (3)(4) and ricefish (medaka, Oryzias latipes) (5)(6)(7).
Veliko izkušenj je bilo na primer pridobljenih s preskusi rasti na cebrici (Danio rerio) (3) (4) in medaki (medaka, Oryzias latipes) (6) (7) (8).
See also General introduction Part C.
Glej tudi Splošni uvod Del C.
Lowest observed effect concentration (LOEC): is the lowest tested concentration of a test substance at which the substance is observed to have a significant effect (at p < 0,05) when compared with the control.
Najnižja koncentracija z opaznim učinkom (Lowest observed effect concentration – LOEC): je najnižja preskušena koncentracija preskusne snovi, pri kateri je bilo opaženo, da ima snov pomemben učinek (pri p < 0,05) v primerjavi s kontrolo.
However, all test concentrations above the LOEC must have a harmful effect equal to or greater than those observed at the LOEC.
Vendar morajo imeti vse preskusne koncentracije nad LOEC škodljiv učinek, ki je enak ali večji kot učinek, opažen pri LOEC.
No observed effect concentration (NOEC): is the test concentration immediately below the LOEC.
Koncentracija brez opaznih učinkov (No observed effect concentration – NOEC): je preskusna koncentracija tik pod LOEC.
ECx: in this test method is the concentration of the test substance which causes a x % variation in growth rate of the fish when compared with controls.
ECx: v tej preskusni metodi je to koncentracija preskusne snovi, ki povzroča x % variacije v stopnji rasti rib, kadar jo primerjamo s kontrolo.
Loading rate: is the wet weight of fish per volume of water.
Stopnja obremenitve: je mokra teža rib na prostornino vode.
Stocking density: is the number of fish per volume of water.
Gostota rib: je število rib na prostornino vode.
Individual fish specific growth rate: expresses the growth rate of one individual based on its initial weight.
Specifična stopnja rasti posameznih rib: izraža stopnjo rasti posamezne ribe na osnovi njene začetne teže.
Tank-average specific growth rate: expresses the mean growth rate of a tank population at one concentration.
Povprečna specifična stopnja rasti v bazenu: izraža srednjo stopnjo rasti populacije bazena pri eni koncentraciji.
Pseudo specific growth rate: expresses the individual growth rate compared to the mean initial weight of the tank population.
Psevdo-specifična stopnja rasti: izraža stopnjo rasti posamezne ribe v primerjavi s srednjo začetno težo populacije bazena.
Juvenile fish in exponential growth phase are placed, after being weighted, in test chambers and are exposed to a range of sublethal concentrations of the test substance dissolved in water preferably under flow-through, or, if not possible, under appropriate semi-static (static-renewal) conditions.
Mladice v fazi eksponentne rasti se po tehtanju dajo v preskusne komore in izpostavijo nizu subletalnih koncentracij preskusne snovi, ki je raztopljena v vodi, po možnosti s pretokom, ali, če to ni mogoče, pod ustreznimi polstatičnimi pogoji (statični pogoji s periodičnim obnavljanjem).
The test duration is 28 days.
Preskus traja 28 dni.
Fish are fed daily.
Ribe se hranijo vsak dan.
The food ration is based on initial fish weights and may be recalculated after 14 days.
Obrok hrane temelji na začetni teži rib in se lahko ponovno preračuna po 14 dneh.