natgillin/translations-raw huggingface dataset:
Gradient systems having a lower dead volume (e.g. 2 ml) should use 22 minutes as an optimal retention time
Gradientni sistemi z manjšo mrtvo prostornino (npr. 2 ml) naj uporabijo 22 minut kot optimalen retenzijski čas.
Take solutions of the standard samples (5.4) without and with 50 % rennet whey.
Odvzem raztopine standardnih vzorcev (5.4) brez siriščne sirotke in s 50 % siriščne sirotke.
Inject 100 μl of supernatant or filtrate (8.3.3) into the HPLC apparatus operating at the scouting gradient conditions given in Table 1.
Vbrizgajte 100 μl supernatanta ali filtrata (8.3.3) v instrument HPLC, ki deluje pri predlaganih gradientnih pogojih, danih v tabeli 1.
Scouting gradient conditions for optimisation of the chromatography
Predlagani gradientni pogoji za optimizacijo kromatografije
Flow
Pretok
Initial
začetek
Comparison of the two chromatograms should reveal the location of the peak of CMPΑ.
Iz primerjave dveh kromatogramov se razbere lokacija vrha CMPΑ.
Using the formula given below, the initial solvent composition to be used for the normal gradient (see 8.4.3) can be calculated % B = 10 – 2,5 + (13,5 + (RTcmpA – 26) / 6) × 30 / 27 % B = 7,5 + (13,5 + (RTcmpA – 26) / 6) × 1,11
Po navedeni enačbi se lahko izračuna sestava začetnega topila, ki se uporabi za normalni gradient (glej 8.4.3): % B = 10 – 2,5 + (13,5 + (RTcmpA – 26)/6) × 30/27 % B = 7,5 + (13,5 + (RTcmpA – 26)/6) × 1,11
retention time of CMPΑ in the scouting gradient
retenzijski čas CMPΑ v predlaganem gradientu
10 the initial % B of the scouting gradient
10 začetni % B v predlaganem gradientu
2,5 % B at midpoint minus % B at initial in the normal gradient
2,5 % B na sredini minus % B na začetku normalnega gradienta
13,5 midpoint time of the scouting gradient
13,5 srednji čas predlaganega gradienta
26 required retention time of CMPΑ
26 zahtevani retenzijski čas CMPΑ
6 ratio of slopes of the scouting and normal gradient
6 razmerje naklonov predlaganega in normalnega gradienta
30 % B at initial minus % B at 27 minutes in the scouting gradient
30 % B na začetku minus % B pri 27 minutah predlaganega gradienta
27 run-time of the scouting gradient.
27 celotni čas predlaganega gradienta.
Take solutions of the test samples
Odvzem raztopin preskusnih vzorcev
Inject 100 μl of accurately measured supernatant or filtrate (8.3.3) into the HPLC apparatus operating at a flow rate of 1,0 ml of eluent solution (5.2) per minute.
Vbrizgajte 100 μl natančno odmerjenega supernatanta ali filtrata (8.3.3) v instrument HPLC, ki deluje pri pretoku 1,0 ml raztopine eluenta (5.2) na minuto.
The composition of the eluent of the start of the analysis is obtained from 8.4.2.
Sestavo eluenta na začetku analize dobite iz 8.4.2.
It is normally close to A:B = 76:24 (5.2).
Ponavadi je blizu A:B = 76:24 (5.2).
Depending on the internal volume of the pumping system, the next injection can be made 15 minutes after reaching the initial conditions.
Odvisno od notranje prostornine sistema črpalk lahko naslednje vbrizgavanje sledi 15 minut zatem, ko dosežete začetno sestavo.
Note 1. The retention time of the CMPA should be 26 ± 2 minutes.
Opomba 1: Retenzijski čas CMPA naj bi bil 26 ± 2 minuti.
This can be achieved by varying the initial and end conditions of the first gradient.
To je mogoče doseči s spreminjanjem začetnih in končnih pogojev prvega gradienta.
This is essential for proper functioning of the gradient pumping system.
To je nujno za pravilno delovanje gradientnega sistema črpalk.
The standard deviation for the retention time of the CMPA peak should be smaller than 0,1 minutes (n = 10).
Standardni odmik retenzijskega časa CMPA vrha naj bo manjši od 0,1 minute (n = 10).
Note 3. Every five samples the reference sample (5) should be injected and used to calculate a new response factor R. (9.1.1).
Opomba 3: Vsakih pet vzorcev je treba vbrizgati referenčni vzorec (5) in ga uporabiti za izračun novega odzivnega faktorja R (9.1.1).
The results of the chromatographic analysis of the test sample (E) are obtained in the form of a chromatogram in which the CMPA peak is identified by its retention time of about 26 minutes
Rezultati kromatografske analize preskusnega vzorca (E) so dobljeni v obliki kromatograma, v katerem je vrh CMPA prepoznan z retenzijskim časom približno 26 minut.
The integrator (6.11.6) automatically calculates the peak height H of the CMPA peak.
Integrator (6.11.6) samodejno izračuna višino vrha H vrha CMPA.
The baseline location should be checked in every chromatogram.
Na vsakem kromatogramu je treba preveriti položaj bazne linije.
The analysis or the integration should be repeated if the baseline was incorrectly located.
Analizo ali integracijo je treba ponoviti, če je bazna linija nepravilno določena.
Note: If the CMPA peak is sufficiently separated from other peaks valley-to-valley baseline allocation should be used, otherwise use dropping perpendiculars to a common baseline, which should have starting point close to the CMPA peak (thus not at t = 0 min!).
Opomba: Če je vrh CMPA dovolj ločen od drugih vrhov, je treba uporabiti bazno linijo dolina–dolina, v nasprotnem primeru uporabite padajoče navpičnice za splošno bazno linijo, ki mora imeti izhodišče blizu vrha CMPA (torej ne pri t = 0 min).
Use for the standard and the samples the same type integration type and check in case of common baseline its consistency for the samples and the standard.
Za standard in vzorec uporabite enako vrsto integracije in v primeru splošne bazne linije preverite skladnost vrste za vzorce in standard.
It is essential to examine the appearance of each chromatogram prior to quantitative interpretation, in order to detect any abnormalities due either to malfunctioning of the apparatus or the column, or to the origin and nature of the sample analysed.
Pred kvantitativno interpretacijo je treba pregledati videz vsakega kromatograma, da bi tako lahko izsledili kakršne koli nepravilnosti kot posledice slabega delovanja bodisi kromatografa ali kolone ali pa kot posledice izvora in narave analiziranega vzorca.
Apply exactly the procedure described from point 8.2 to point 8.4.4 to the standard samples (5.4.1 to 5.4.2).
Za standardne vzorce uporabite postopek, opisan od točke 8.2 do točke 8.4.4 (od 5.4.1 do 5.4.2).
Use freshly prepared solutions, because CMP degrades in an 8 % trichloroacetic acid environment at room temperature.
Uporabite sveže pripravljene raztopine, ker se CMP razgradi v 8-odstotni triklorocetni kislini pri sobni temperaturi.
In this figure the CMPA peak is preceded by two small peaks.
Na tej sliki sta pred vrhom CMPA dva majhna vrhova.
It is essential to obtain a similar separation.
Nujno je, da dobite podobno ločevanje.
Prior to chromatographic determination of the samples inject 100 μl of the standard sample without rennet whey [0] (5.4.1)
Pred kromatografskim določanjem vzorcev vbrizgajte 100 μl standardnega vzorca brez siriščne sirotke [0] (5.4.1).
The chromatogram should not show a peak at the retention time of the CMPA peak.
Kromatogram ne sme imeti vrha pri retenzijskem času vrha CMPA.
Determine the response factors R by injecting the same volume of filtrate (8.5.1) as used for the samples.
Z vbrizganjem enake prostornine filtrata (8.5.1), kakor je uporabljena pri vzorcih, določite odzivni faktor R.
Calculation of the response factor R:
Izračun odzivnega faktorja R:
CMPA peak: R = W/H
vrh CMPA: R = W/H
R the response factor of the CMPA peak
R odzivni faktor vrha CMPA
H the height of the CMPA peak
H višina vrha CMPA
W the quantity of whey in the standard sample [5].
W količina sirotke v standardnem vzorcu [5].
Calculation of the percentage of rennet whey powder in the sample
Izračun deleža siriščne sirotke v prahu v vzorcu
W(E) the percentage (m/m) of rennet whey in the sample (E).
W(E) masni delež (m/m) siriščne sirotke v vzorcu [E].
R the response factor of the CMPA peak (9.1.1)
R odzivni faktor vrha CMPA (9.1.1)
H(E) the height of the CMPA peak of the sample (E)
H(E) višina vrha CMPA iz vzorca (E)
If W(E) is greater than 1 % and the difference between the retention time and that of the standard sample [5] is smaller than 0,2 minutes then rennet whey solids are present.
Če je W(E) večji od 1 % in razlika med retenzijskim časom pri vzorcu in standardnem vzorcu manjša od 0,2 minute, potem je suha snov siriščne sirotke prisotna.
Not determined.
Ni določena.
From 0 to 16 % of rennet whey a linear relationship should be obtained with a coefficient of correlation > 0,99.
Linearno zvezo s korelacijskim koeficientom > 0,99 je treba dobiti v območju od 0 do 16 % siriščne sirotke.
The 1 % limit is fixed in agreement with the provisions of points 9.2 and 9.4.1 of Annex XIX to Regulation (EEC) No 214/2001, which includes the uncertainty due to reproducibility.
Meja 1 % je postavljena v skladu z zahtevami točk 9.2 in 9.4.1 Priloge XIX k Uredbi (EGS) št. 214/2001, ki vključuje negotovost zaradi obnovljivosti.
Absorbance (220 nm)
Absorbanca (220 nm)
times (Minutes)
Čas (v minutah)
Rennet-type whey powder of standard composition and also the adulterated skimmed-milk powder are available from NIZO, Kernhemseweg 2, PO Box 20 — NL-6710 BA Ede.
Prah siriščne sirotke standardne sestave in potvorjeno posneto mleko v prahu sta razpoložljiva pri NIZO, Kernhemseweg 2, PO Box 20 – NL-6710 BA Ede.
However, powders giving equivalent results to the NIZO powders may also be used.
Vendar se lahko uporabi tudi drug prah, ki daje enake rezultate kot prah NIZO.
(Article 10)
(Člen 10)
SKIMMED-MILK POWDER: QUANTITATIVE DETERMINATION OF PHOSPHATIDYLSERINE AND PHOSPHATIDYLETHANOLAMINE
POSNETO MLEKO V PRAHU: KOLIČINSKO DOLOČANJE FOSFATIDILSERINA IN FOSFATIDILETANOLAMINA
Method: reversed-phase HPLC
Metoda: HPLC na reverzni fazi
PURPOSE AND FIELD OF APPLICATION
NAMEN IN PODROČJE UPORABE
The method describes a procedure for the quantitative determination of phosphatidylserine (PS) and phosphatidylethanolamine (PE) in skimmed-milk powder (SMP) and is suitable for detecting buttermilk solids in SMP.
Metoda navaja postopek za količinsko določanje fosfatidilserina (PS) in fosfatidiletanolamina (PE) v posnetem mleku v prahu (SMP) in je primerna za ugotavljanje trdnega pinjenca v SMP.
PS + PE content: the mass fraction of substance determined using the procedure here specified.
Vsebnost PS + PE: masni delež snovi, določen s tukaj opisanim postopkom.
The result is expressed as milligrams of phosphatidylethanolamine dipalmitoyl (PEDP) per 100 g powder.
Rezultat se izrazi v miligramih fosfatidiletanolamina dipalmitola (PEDP) na 100 g prahu.
Extraction of aminophospholipids by methanol from reconstituted milk powder.
Ekstrakcija aminofosfolipidov iz rekonstruiranega mleka v prahu.
Determination of PS and PE as o-phthaldialdehyde (OPA) derivatives by reversed-phase (RP) HPLC and fluorescence detection.
Določitev PS in PE kot derivatov o-ftaldialdehida (OPA) s HPLC na reverzni fazi (RP) in fluorescenčno detekcijo.
Quantification of PS and PE content in the test sample by reference to a standard sample containing a known amount of PEDP.
Količinska opredelitev vsebnosti PS in PE v preskusnem vzorcu glede na standardni vzorec, ki vsebuje znano količino PEDP.
Water must be distilled or water or water of at least equivalent purity, unless otherwise specified.
Uporabljena voda mora biti destilirana ali vsaj primerljive čistosti, če ni navedeno drugače.
Standard material: PEDP, at least 99 % pure
Standardni material: PEDP s čistostjo najmanj 99 %
Note: Standard material must be stored at –18 °C.
Opomba: Standardni material je treba hraniti pri –18 °C.
Reagents for standard sample and test sample preparation
Reagenti za pripravo standardnega in preskusnega vzorca
HPLC-grade methanol
Metanol, HPLC-čistost
HPLC-grade chloroform
Kloroform, HPLC-čistost
Reagents for o-phthaldialdehyde derivatisation
Reagenti za o-ftalaldehidno derivatizacijo
Sodium hydroxide, 12 M water solution
Natrijev hidroksid, 12 M vodna raztopina
Boric acid, 0,4 M water solution adjusted to pH 10,0 with sodium hydroxide (4.3.1)
Borna kislina, 0,4 M vodna raztopina, uravnana na pH 10,0 z natrijevim hidroksidom (4.3.1)
o-phthaldialdehyde (OPA)
o-ftalaldehid (OPA)
HPLC elution solvents
Reagenti za HPLC (eluenti)
Elution solvents must be prepared using HPLC-grade reagents.
Eluente je treba pripraviti z uporabo reagentov HPLC-čistost.
HPLC-grade water
Voda, HPLC-čistost
Methanol of fluorimetric tested purity
Metanol, čistost za fluorimetrijo
Acetic acid.
Ocetna kislina
Analytical balance, capable of weighing to the nearest 1 mg, with a readability of 0,1 mg
Analitska tehtnica, ki lahko tehta na 1 mg natančno, s čitljivostjo 0,1 mg.
Beakers, 25 and 100 ml capacity
Čaše s prostornino 25 in 100 ml
Pipettes, capable of delivering 1 and 10 ml
Pipete, ki lahko prepuščajo 1 ml in 10 ml
Graduated pipettes, capable of delivering 0,2, 0,5 and 5 ml
Polnilne pipete, ki lahko prepuščajo 0,2, 0,5 in 5 ml
Volumetric flasks, 10, 50 and 100 ml capacity
Merilne bučke s prostornino 10, 50 in 100 ml
Syringes, 20 and 100 μl capacity
Brizgalke s prostornino 20 in 100 μl
Centrifuge, capable of operating at 27000 × g
Centrifuga, delujoča pri 27000 × g
Glass vials, about 5 ml capacity
Reakcijske stekleničke s prostornino približno 5 ml
Graduated cylinder, 25 ml capacity
Merilni valj s prostornino 25 ml
pH-meter, accurate to 0,1 pH units
pH-meter z natančnostjo do 0,1 pH-enot
HPLC equipment
HPLC-oprema
Gradient pumping system, capable of operating at 1,0 ml/min at 200 bar
Gradientni sistem črpalk z možnostjo delovanja pri pretoku 1,0 ml/min pri 200 barih
Autosampler with derivatisation capability
Avtomatski vzorčevalnik z možnostjo izvajanja derivatizacije
Column heater, capable of maintaining the column at 30 °C ± 1 °C
Grelnik kolone, ki lahko temperaturo ohranja na 30 °C ± 1 °C
Fluorescence detector, capable of operating at 330 nm excitation wavelength and 440 nm emission wavelength
Fluorescenčni detektor z valovno dolžino vzbujanja 330 nm in valovno dolžino emisije 440 nm
Integrator or data processing software capable of peak area measurement
Integrator ali programska oprema za obdelavo podatkov, ki lahko meri površino vrha
A Lichrosphere — 100 column (250 × 4,6 mm) or an equivalent column packed with octadecylsilane (C 18), 5 μm particle size.
Kolona Lichrosphere – 100 (250 × 4,6 mm) ali enakovredna kolona, polnjena z oktadecilsilanom (C 18), velikost delcev 5 μm
Sampling must be carried out in accordance with ISO Standard 707.
Vzorčenje mora biti izvedeno v skladu s standardom ISO 707.
Preparation of the internal standard solution
Priprava raztopine internega standarda
Weigh 30,0 ± 0,1 mg of tryptamine-monohydrochloride (4.2.3) into a 100 ml volumetric flask (5.6) and make up to the mark with methanol (4.2.1)
Natehtajte 30,0 ± 0,1 mg triptamin-monohidroklorida (4.2.3) v 100-mililitrsko merilno bučko (5.6) in dopolnite do oznake z metanolom (4.2.1).
Pipette 1 ml (5.3) of this solution into a 10 ml volumetric flask (5.6) and make up to the mark with methanol (4.2.1) in order to obtain a 0,15 mM tryptamine concentration
Odpipetirajte 1 ml (5.3) te raztopine v 10-mililitrsko merilno bučko (5.6) in dopolnite do oznake z metanolom (4.2.1), da dobite raztopino s koncentracijo 0,15 mM triptamina.
Preparation of the test sample solution
Priprava raztopine preskusnega vzorca
Weigh 1,000 ± 0,001 g of the SMP sample into a 25 ml beaker (5.2).
Odtehtajte 1,000 ± 0,001 g vzorca SMP v 25-mililitrsko čašo (5.2).
Add 10 ml of distilled water at 40 °C ± 1 °C by a pipette (5.3) and stir with a magnetic stirrer (5.4) for 30 minutes in order to dissolve any lumps
S pipeto (5.3) dodajte 10 ml destilirane vode pri 40 °C ± 1 °C in mešajte 30 minut z magnetnim mešalnikom (5.4), da raztopite morebitne grudice.
Pipette 0,2 ml (5.5) of the reconstituted milk into a 10 ml volumetric flask (5.6), add 100 μl of the 0,15 mM tryptamine solution (7.1) using a syringe (5.7) and make up to the volume with methanol (4.2.1).
Odpipetirajte 0,2 ml (5.5) rekonstruiranega vzorca mleka v 10-mililitrsko merilno bučko (5.6), dodajte 100 μl 0,15 mM raztopine triptamina (7.1) z brizgalko (5.7) in dopolnite do oznake z metanolom (4.2.1).
Mix carefully by inversion and sonicate (5.8) for 15 min
Previdno zmešajte z obračanjem bučke in postavite v ultrazvočno kopel (5.8) za 15 min.
Centrifuge (5.9) at 27000 g × g for 10 minutes and collect the supernatant in a glass vial (5.10)
Centrifugirajte (5.9) 10 minut pri 27000 g in zberite bistro tekočino v stekleno vialo (5.10).
Note: Test sample solution should be stored at 4 °C until the HPLC analysis is performed.
Opomba: Raztopino preskusnega vzorca je treba do HPLC-analize hraniti pri 4 °C.
Preparation of the external standard solution
Priprava raztopine eksternega standarda
Weigh 55,4 mg PEDP (4.1) into a 50 ml volumetric flask (5.6) and add about 25 ml of chloroform (4.2.2) using a graduated cylinder (5.11).
Odtehtajte 55,4 mg PEDP (4.1) v 50-mililitrsko merilno bučko (5.6) in dodajte z merilnim valjem (5.11) približno 25 ml kloroforma (4.2.2).
Heat the stoppered flask to 50 °C ± 1 °C and mix carefully till the PEDP dissolves.
Zaprto bučko segrejte do 50 °C ± 1 °C in previdno premešajte, da se PEDP raztopi.
Cool the flask to 20 °C, make up to the volume with methanol (4.2.1) and mix by inversion
Ohladite bučko na 20 °C in dopolnite do oznake z metanolom (4.2.1) ter zmešajte z obračanjem.
Pipette 1 ml (5.3) of this solution into a 100 ml volumetric flask (5.6) and make up to the volume with methanol (4.2.1).
Odpipetirajte 1 ml (5.3) te raztopine v 100-mililitrsko merilno bučko (5.6) in dopolnite do oznake z metanolom (4.2.1).
Pipette 1 ml (5.3) of this solution into a 10 ml volumetric flask (5.6), add 100 μl (5.7) of 0,15 mM tryptamine solution (7.1) and make up to the volume with methanol (4.2.1).
Odpipetirajte 1 ml (5.3) te raztopine v 10-mililitrsko merilno bučko (5.6), dodajte 100 μl (5.7) 0,15 mM raztopine triptamina (7.1) in dopolnite do oznake z metanolom (4.2.1).
Mix by inversion
Zmešajte z obračanjem bučke.
Note: Reference sample solution should be stored at 4 °C until the HPLC analysis is performed.
Opomba: Raztopino referenčnega vzorca je treba do HPLC-analize hraniti pri 4 °C.
Preparation of the derivatising reagent
Priprava reagenta za derivatizacijo
Weigh 25,0 ± 0,1 mg of OPA (4.3.4) into a 10 ml volumetric flask (5.6), add 0,5 ml (5.5) of methanol (4.2.1) and mix carefully to dissolve the OPA.
Odtehtajte 25,0 ± 0,1 mg OPA (4.3.4) v 10-mililitrsko merilno bučko (5.6), dodajte 0,5 ml (5.5) metanola (4.2.1) in previdno premešajte, da se OPA raztopi.
Make up to the mark with boric acid solution (4.3.2) and add 20 μl of 2-mercaptoethanol (4.3.3) by syringe (5.7).
Dopolnite do oznake z raztopino borne kisline (4.3.2) in dodajte z brizgalko (5.7) 20 μl 2-merkaptoetanola (4.3.3).
Note: The derivatising reagent should be stored at 4 °C in a brown glass vial and is stable for one week.
Opomba: Reagent za derivatizacijo je treba hraniti v temni viali pri 4 °C in je stabilen en teden.
Elution solvents (4.4)
Topila (eluenti) (4.4)
Solvent A: Solution of 0,3 mM sodium dihydrogen phosphate and 3 mM sodium acetate solution (adjusted to pH 6,5 ± 0,1 with acetic acid): methanol: tetrahydrofuran = 558:440:2 (v/v/v)
Topilo A: 0,3 mM raztopina natrijevega dihidrogen fosfata in 3 mM natrijevega acetata (pH uravnan na 6,5 ± 0,1 z ocetno kislino):metanol:tetrahidrofuran = 558:440:2 (v/v/v)
Solvent B: methanol
Topilo B: metanol.
Suggested eluting gradient:
Predlagani elucijski gradient
Solvent A
Topilo A
Solvent B
Topilo B
Flow rate
Pretok
0Note: The eluting gradient may require slight modification in order to achieve the resolution shown in figure 1.
0Opomba: Elucijski gradient je morda treba rahlo spremeniti, da bi dobili ločitev, kakor je prikazana na sliki 1.
Injection volume: 50 μl derivatising reagent and 50 μl sample solution
Prostornina vbrizganja: 50 μl reagenta za derivatizacijo in 50 μl raztopine vzorca
Column equilibration
Vzpostavitev ravnotežja v koloni
Starting up the system on a daily basis, flush the column with 100 % solvent B for 15 minutes, then set at A:B = 40:60 and equilibrate at 1 ml/min for 15 minutes.
Če se sistem začne dnevno, se kolona spira 15 minut s 100-odstotnim topilom B, potem nastavi A:B = 40:60 in 15 minut vzpostavlja ravnotežje pri pretoku 1 ml/min.
Perform a blank run by injecting methanol (4.2.1).
Slepa določitev se izvede z vbrizganjem metanola (4.2.1).
Note: Before long-term storage flush the column with methanol: chloroform = 80:20 (v/v) for 30 minutes.
Opomba: Pred daljšim hranjenjem je treba kolono spirati 30 minut z mešanico metanol:kloroform = 80:20 (v/v).
Determine the PS + PE content in the test sample
Določitev vsebnosti PS + PE v preskusnem vzorcu
Perform the sequence of the chromatographic analyses keeping constant the run-to-run time in order to obtain constant retention times.
Izvedite zaporedje kromatografskih analiz s konstantnim časom med dvema analizama, da s tem zagotovite konstanten retenzijski čas.
Inject the external standard solution (7.3) every 5-10 test sample solutions in order to calculate the response factor 0
Vsakih 5–10 preskusnih vzorcev vbrizgajte raztopino eksternega standarda (7.3) za ovrednotenje odzivnega faktorja0
Note: The column must be cleaned by flushing with 100 % solvent B (7.5.1) for at least 30 minutes every 20-25 runs.
Opomba: Na vsakih 20–25 analiz je nujno kolono najmanj 30 minut spirati s 100-odstotnim topilom B.
Integration mode
Način integracije
PEDP peak
Vrh PEDP
PEDP is eluted as a single peak.
Vrh PEDP se pojavi samostojno.
Determine the peak area by valley-to-valley integration.
Njegova površina se določi z integracijo dolina-dolina.
Tryptamine peak
Vrh triptamina
Tryptamine is eluted as a single peak (Figure 1).
Vrh triptamina se pojavi samostojno (slika 1).
PS and PE peaks groups
Skupine vrhov PS in PE
Under the described conditions (Figure 1), PS elutes as two main partially unresolved peaks preceded by a minor peak.
Pri opisanih pogojih (slika 1) se PS eluira v obliki dveh delno neločenih vrhov, pred katerima je še en manjši vrh.
PE elutes as three main partially unresolved peaks.
PE se eluira v obliki treh glavnih delno neločenih vrhov.
Determine the whole area of each peak cluster setting the baseline as reported in Figure 1.
Površina vsake skupine vrhov se določi z nastavitvijo bazne linije, kakor je prikazano na sliki 1.
CALCULATION AND EXPRESSION OF RESULTS
IZRAČUN IN IZRAŽANJE REZULTATOV
PS and PE content in the test sample shall be calculated as follows: C = 55,36 × ((A2)/(A1)) × ((T1)/(T2))
Vsebnost PS in PE v preskusnem vzorcu se izračuna, kakor sledi: C = 55,36 × ((A2)/(A1)) × ((T1)/(T2))
C PS or PE content (mg/100 g powder) in the test sample
C vsebnost PS ali PE (v mg/100 g prahu) v preskusnem vzorcu
PEDP peak area of the standard sample solution (7.3)
površina vrha PEDP raztopine standardnega vzorca (7.3)
PS or PE peak area of the test sample solution (7.2)
površina vrha PS ali PE raztopine preskusnega vzorca (7.2)
Tryptamine peak area of the standard sample solution (7.3)
površina vrha triptamina v raztopini standardnega vzorca (7.3)
Tryptamine peak area of the test sample solution (7.2).
površina vrha triptamina v raztopini preskusnega vzorca (7.2)
Note: The values for repeatability were calculated according to the IDF International Standard [1].
Opomba: Vrednosti za ponovljivost so bile izračunane v skladu z mednarodnim standardom IDF [1].
The provisional reproducibility limit was calculated according to the procedure defined in Annex III(b) hereto.
Predpisana meja za obnovljivost je bila izračunana v skladu s postopkom iz Priloge III(b).
The relative standard derivation of the repeatability, which expresses the variability of independent analytical results obtained by the same operator using the same apparatus under the same conditions on the same test sample and in a short interval of time, should not exceed 2 % relative.
Relativni standardni odmik ponovljivosti, izražajoč variabilnost neodvisnih analitskih rezultatov, ki jih dobi isti analitik z isto opremo pri enakih pogojih na istem preskusnem vzorcu v kratkem časovnem intervalu, ne sme presegati vrednosti 2 % relativno.