natgillin/translations-raw huggingface dataset:
‘Determination of free fatty acids, cold method’
„Določevanje prostih maščobnih kislin, metoda v hladnem“;
the title of Annex VII is replaced by the following:
naslov Priloge VII se nadomesti z naslednjim:
‘Determination of the percentage of 2-glyceryl monopalmitate’;
„Določevanje odstotnega deleža 2-gliceril monopalmitata“;
OLIVE OIL CHARACTERISTICS
LASTNOSTI OLJČNEGA OLJA
The results of the analyses must be expressed to the same number of decimal places as used for each characteristic.
Rezultate analiz je treba navesti podati na enako isto število decimalk natančno, kot je za določeno za posamezno lastnost.
The last digit must be increased by one unit if the following digit is greater than 4.
Zadnjo števko v številki je treba povečati za eno enoto, če je naslednja številka večja od prekorači 4.
If just a single characteristic does not match the values stated, the category of an oil can be changed or the oil declared impure for the purposes of this Regulation.
Če samo ena lastnost značilnost ni v skladu z navedenimi vrednostmi, se olje razvrsti v drugo kategorijo ali se šteje za neustrezno glede čistost ini deklarirano kategorijo.
If a characteristic is marked with an asterisk (*), referring to the quality of the oil, this means the following:
Značilnosi, označene z zvezdico (*), nanašajoč se na kakovost olja, pomenijo:
for lampante olive oil, it is possible for both the relevant limits to be different from the stated values at the same time;
za oljčno olje lampante so lahko ustrezne mejne vrednosti hkrati različne od navedenih vrednosti,
for virgin olive oils, if at least one of these limits is different from the stated values, the category of the oil will be changed, although they will still be classified in one of the categories of virgin olive oil.
za deviška oljčna olja je nespoštovanje samo ene od mejnih vrednosti vzrok za spremembo kategorije, vendar olje ostane razvrščeno v eno od kategorij deviških oljčnih olj.
If a characteristic is marked with two asterisks (**), referring to the quality of the oil, this means that for all types of olive-residue oil, it is possible for both the relevant limits to be different from the stated values at the same time.’
Če je značilnost označena z dvema zvezdicama (**), nanašajoč se na kakovost olja, to pomeni, da so lahko za vsa olja iz oljčnih tropin mejne vrednosti hkrati različne od navedenih vrednosti.“
Peroxide index
Peroksidno število
Waxes
Voski
2-glyceril monopalmitate
2-gliceril monopalmitat
Stigmastadiene
Stigmastadiena
Difference: ECN42 (HPLC) and ECN42 (theoretical calculation)
Razlika med HPLC ECN42 in teoretičnim izračunom ECN42
Organoleptic evaluation median defect
Organoleptično ocenjevanje Mediana napak
Organoleptic evaluation fruity median
Organoleptično ocenjevanje Mediana sadežnosti
Extra virgin olive oil
Ekstra deviško oljčno olje
≤ 0,9 if total palmitic acid % ≤ 14
≤ 0,9 če je skupni % palmitinske kisline ≤ 14%
≤ 1,0 if total palmitic acid % > 14
≤1,0 če je skupni % palmitinske kisline > 14 %
Lampante olive oil
Lampante oljčno olje
≤ 1,1 if total palmitic acid % > 14
≤ 1,1 če je skupni % palmitinske kisline > 14%
Refined olive oil
Rafinirano oljčno olje
Olive oil composed of refined and virgin olive oils
Oljčno olje - mešanica rafiniranih oljčnih olj in deviških oljčnih olj
Crude olive-residue oil
Surovo olje iz oljčnih tropin
Refined olive-residue oil
Rafinirano olje iz oljčnih tropin
Olive-residue oil
Olje iz oljčnih tropin
Acid content [5]
Vsebnost kislin [5]
Total transoleic isomers
Vsota translinolnih izomer
Total translinoleic + translinolenic isomers
Vsote translinolenih in translinolenskih izomer
Sterols composition
Sestava sterolov
Total sterols
Skupni steroli
Erythrodiol and uvaol
Eritrodiol in uvaol
Arachidic
Arašidova
Eicosenoic
Eikozanojska
Total isomers which could (or could not) be separated by capillary column.
Vsota skupnih ali posameznih izomerov, ki jih določimo s kapilarno kolono.
Or where the median defect is less than or equal to 2,5 and the fruity median is equal to 0.
Ali če je mediana napake manjša ali enaka 2,5 in je mediana sadežnosti 0.
Oils with a wax content of between 300 mg/kg and 350 mg/kg are considered to be lampante olive oil if the total aliphatic alcohol content is less than or equal to 350 mg/kg or if the erythrodiol and uvaol content is less than or equal to 3,5 %.
Olja z vsebnostjo voskov med 300 mg/kg in 350 mg/kg se uvrščajo med lampante oljčna olja, če je vsebnost alifatskih alkoholov manjša ali enaka 350 mg/kg ali če je delež eritrodiola in uvaola manjši ali enak 3,5 %.
Oils with a wax content of between 300 mg/kg and 350 mg/kg are considered to be crude olive-residue oil if the total aliphatic alcohol content is above 350 mg/kg and if the erythrodiol and uvaol content is greater than 3,5 %.
Olja z vsebnostjo voskov med 300 mg/kg in 350 mg/kg se uvrščajo med surova olja iz oljčnih tropin, če je skupna vsebnost alifatskih alkoholov večja od 350 mg/kg in če je delež eritrodiola in uvaola večji od 3,5 %.
Other fatty acids content (%): palmitic: 7,5-20,0; palmitoleic: 0,3-3,5; heptadecanoic: ≤ 0,3; heptadecenoic: ≤ 0,3; stearic: 0,5-5,0; oleic: 55,0-83,0; linoleic: 3,5-21,0.
Vsebnost drugih maščobnih kislin (%): palmitinske: 7,5–20,0; palmitoleinske: 0,3–3,5; heptadekanojske ≤ 0,3; heptadecenojske: ≤ 0,3; stearinske: 0,5–5,0; oleinske: 55,0–83,0; linolenske: 3,5–21,0.
Total: Delta-5,23-stigmastadienol+chlerosterol+beta-sitosterol+sitostanol+delta-5-avenasterol+delta-5,24-stigmastadienol.
Vsota: delta-5,23-stigmastadienola+klerosterola+beta-sitosterola+sitostanola+delta-5-avenasterola+delta-5,24-stigmastadienola.
Oils with a wax content of between 300 mg/kg and 350 mg/kg are considered to be lampante olive oil if the total aliphatic alcohol content is less than or equal to 350 mg/kg or if the erythrodiol and uvaol content is less than or equal to 3,5 %.
Olja z vsebnostjo voskov med 300 mg/kg in 350 mg/kg se uvrščajo med lampante oljčna olja, če je skupna vsebnost alifatskih alkoholov manjša ali enaka 350 mg/kg ali če je delež eritrodiola in uvaola manjši ali enak 3,5 %.
Determination of free fatty acids, cold method’
Določevanje prostih maščobnih kislin, metoda v hladnem“;
the thirteenth indent is replaced by the following:
trinajsta alinea se nadomesti z naslednjim:
Saturated fatty acids in position 2
Nasičene maščobne kisline na položaju 2
Determination of the percentage of 2-glyceryl monopalmitate’;
Določevanje odstotnega deleža 2-gliceril monopalmitata“;
‘DETERMINATION OF FREE FATTY ACIDS, COLD METHOD’;
„DOLOČEVANJE PROSTIH MAŠČOBNIH KISLIN, METODA V HLADNEM“;
DETERMINATION OF WAX CONTENT BY CAPILLARY COLUMN GAS CHROMATOGRAPHY
DOLOČEVANJE VSEBNOSTI VOSKOV S KAPILARNO PLINSKO KROMATOGRAFIJO
SUBJECT
NAMEN
This method describes a process for determining the wax content of olive oils.
Ta metoda opisuje postopek za določitev vsebnosti voskov v oljčnem olju.
Waxes are separated according to the number of their carbon atoms.
Voski se ločijo glede na število ogljikovih atomov.
The method may be used in particular to distinguish between olive oil obtained by pressing and that obtained by extraction (olive-residue oil).
Metodo lahko uporabimo zlasti za razlikovanje med oljčnim oljem, pridobljenim s stiskanjem, in oljčnim oljem, pridobljenim z ekstrakcijo (olje iz oljčnih tropin).
25 ml Erlenmeyer flask.
Erlenmajerica prostornine 25 ml.
Glass column for gas chromatography, internal diameter 15,0 mm, length 30 to 40 cm, fitted with a stopcock.
Steklena kolona za plinsko kromatografijo, z notranjim premerom 15,0 mm, dolžine 30 do 40 cm in opremljena s petelinčkom.
Suitable gas chromatograph with a capillary column, equipped with a system for direct introduction into the column comprising the following:
Plinski kromatograf, primeren za uporabo s kapilarno kolono in opremljen s sistemom za neposredno injiciranje v kolono, ki obsega:
Thermostatic chamber for the columns, equipped with a temperature programmer.
termostatsko kontrolirano komoro za kolone, s programatorjem temperature;
Cold injector for direct introduction into the column.
hladen injektor za injiciranje neposredno v kolono;
Flame ionisation detector and converter-amplifier.
plamensko ionizacijski detektor in konverter-ojačevalec;
Recorder-integrator capable of working with the converter-amplifier (3.3.3), rate of response no slower than 1 second, with variable paper speed.
rekorder-integrator, primeren za uporabo s konverterjem-ojačevalcem (3.3.3), z odzivnim časom, manjšim od 1 sekunde, in nastavljivo hitrostjo pomika papirja.
(It is also possible to use computerised systems that allow the acquisition of gas chromatography data via a PC.)
(Uporabimo lahko tudi računalniške sisteme, pri katerih je predvideno zajemanje podatkov plinske kromatografije prek osebnega računalnika.);
Glass or fused silica capillary column 8 to 12 m long and with an internal diameter of 0,25 to 0,32 mm, with liquid phase, with a uniform film thickness between 0,10 and 0,30 μm.
Steklena ali kvarčna kapilarna kolona dolžine 8 do 12 m, z notranjim premerom 0,25 do 0,32 mm, znotraj pokrita s tekočo stacionarno fazo in enotne debeline 0,10 do 0,30 μm.
(There are liquid phases suitable for the purpose of type SE-52 or SE-54 available on the market.)
(Tekoče stacionarne faze, namenjene uporabi, v prodaji vrste SE 52 ali SE 54.)
10 μl microsyringe for on-column injection, equipped with a hardened needle.
10 μl-mikrobrizgalka s kaljeno iglo in z možnostjo neposrednega injiciranja v kolono.
Electrovibrator.
Električni vibrator.
Rotary evaporator.
Rotavapor.
Muffle furnace.
Žarilna peč.
Analytical balance with guaranteed precision of ± 0,1 mg.
Analitska tehtnica, ki zagotavlja ± 0,1 mg natančnost.
Normal laboratory glassware.
Običajna laboratorijska steklovina.
REAGENTS
REAGENTI
Silica gel with a granule size of between 60 and 200 μm.
Silikagel z velikostjo delcev med 60 in 200 μm.
Place the gel in the furnace at 500 °C for at least four hours.
Silikagel vsaj 4 ure žarimo pri 500 °C.
After cooling, add 2 % water in relation to the quantity of sampled silica gel.
Nato ga ohladimo in dodamo 2 % vode glede na količino silikagela.
Shake well to homogenise the slurry.
Dobro premešamo, da se zmes homogenizira.
Keep in darkness for at least 12 hours prior to use.
Pred uporabo hranimo vsaj 12 ur v temi.
n-hexane, for chromatography.
n-heksan, kromatografske čistoče.
Ethyl ether, for chromatography.
Dietil eter, kromatografske čistoče.
n-heptane, for chromatography.
n-heptan, kromatografske čistoče.
Standard solution of lauryl arachidate, at 0,1 % (m/v) in hexane (internal standard).
Standardna raztopina lauril arahidata koncentracije 0,1 % (m/V) v heksanu (interni standard).
(It is also possible to use palmityl palmitate or myristyl stearate.)
(Lahko uporabimo tudi -palmitil palmitat ali miristil stearat.)
Sudan 1 (1-phenyl-azo-2-naphthol).
Sudan 1 (1-fenilazo-2-naftol).
Carrier gas: hydrogen or helium, gas-chromatographic purity.
Nosilni plin: vodik ali helij, čist, plinsko-kromatografske čistoče.
Auxiliary gases:
Pomožni plini:
pure hydrogen for gas chromatography,
vodik, čist, plinsko-kromatografske čistoče,
pure air for gas chromatography.
zrak, čist, plinsko-kromatografske čistoče.
Preparation of the chromatographic column.
Priprava kromatografske kolone
Complete settling with the aid of an electrovibrator (3.5) to make the chromatographic layer more homogeneous.
Homogenost posedanja, ki poveča homogenost kromatografskih pasov, lahko dosežemo z električnim vibratorjem (3.5).
Percolate 30 ml of n-hexane to remove any impurities.
Spiramo s 30 ml n-heksana, da odstranimo vse morebitne nečistoče.
Using the balance (3.8) weigh exactly 500 mg of the sample into the 25 ml Erlenmeyer flask (3.1), add the appropriate quantity of the internal standard (4.5) according to the presumed wax content.
S tehtnico (3.8) v erlenmajerico prostornine 25 ml (3.1) natehtamo natanko 500 mg vzorca in dodamo ustrezno količino internega standarda (4.5), ki je odvisna od pričakovane vsebnosti voskov.
For example, add 0,1 mg of lauryl arachidate for olive oil, and 0,25 to 0,5 mg for olive-residue oil.
Na primer, pri oljčnem olju dodamo 0,1 mg, pri olju iz oljčnih tropin pa 0,25 do 0,5 mg lauril arahidata.
Transfer the prepared sample to the chromotography column using two 2 ml portions of n-hexane (4.2).
Tako pripravljen vzorec prenesemo v kromatografsko kolono, in sicer z dvema 2-ml odmerkoma n-heksana (4.2).
Allow the solvent to flow away until it reaches 1 mm above the upper level of the absorbant then percolate a further 70 ml of n-hexane in order to eliminate the n-alkanes naturally present.
Topilo izpuščamo iz kolone tako dolgo, da doseže 1 mm nad zgornjim robom absorbenta, zatem spiramo z dodatnimi 70 ml n-heksana, da odstranimo morebitne naravno prisotne n-alkane.
Then start the chromatographic elution by collecting 180 ml of the mixture of n-hexane/ethyl ether (ratio 99:1), keeping a rate of flow of approximately 15 drops every 10 seconds.
Nato začnemo kromatografsko eluiranje, in sicer zberemo 180 ml mešanice n-heksan/etilni eter v razmerju 99:1 in pri pretoku približno 15 kapljic na 10 sekund.
Elution of the sample must be carried out at a room temperature of 22 ± 4 °C.
Eluiranje vzorca mora potekati pri sobni temperaturi 22 ± 4 °C.
The n-hexane/ethyl ether mixture (99:1) must be prepared every day.
Dnevno je treba pripraviti svežo mešanico n-heksana/etilnega etra (99:1).
For a visual check on the correct elution of the waxes 100 μl of 1 % Sudan in the elution mixture can be added to the sample in solution.
Za opazovanje pravilnega eluiranja voskov lahko vzorcu v raztopini dodamo 100 μl 1-odstotnega sudana v elucijski mešanici.
Since the colourant has an intermediate retention, between waxes and triglycerides, when the coloration has reached the bottom of the column the elution should be suspended because all the waxes will have been eluted.
Ker ima barvilo vmesno retencijo med voski in trigliceridi, je treba elucijo suspendirati, ko obarvanje doseže dno kromatografske kolone, ker so se eluirali vsi voski.
Dry the fraction thus obtained in a rotary evaporator (3.6.) until virtually all the solvent has been eliminated.
Iz tako dobljene frakcije na rotavaporju (3.6) odparimo skoraj vse topilo.
Eliminate the final 2 ml of solvent with the aid of a weak current of nitrogen; then add 2-4 ml n-heptane.
Preostala 2 ml topila odstranimo z uporabo blagega toka dušika, nato dodamo 2-4 ml n-heptana.
Analysis by gas chromatography
Plinsko-kromatografska analiza
Preparatory work
Predpriprava
Fit the column to the gas chromatograph (3.3) by connecting the inlet port to the on-column system and the outlet port to the detector.
Kolono pritrdimo na plinski kromatograf (3.3), in sicer vstopni del na injektor, izhodnega pa na detektor.
Perform a general check on the GC apparatus (operation of gas circuits, detector and recorder efficiency, etc.).
Izvedemo splošno preverjanje plinskega kromatografa (tesnjenje plinskih povezav, pravilno delovanje detektorja in rekorderja itd.).
If the column is being used for the first time it should be conditioned first.
Če kolono uporabljamo prvič, je priporočljivo, da jo kondicioniramo.
Pass a little gas through the column, then turn on the GC apparatus.
Pri majhnem pretoku plina skozi kolono vključimo plinski kromatograf.
Heat gradually until 350 °C is reached after about four hours.
Postopoma segrevamo, tako da po približno 4 urah dosežemo temperaturo 350 °C.
Maintain that temperature for at least two hours then regulate the apparatus to operating conditions (set gas flow, light flame, connect to the electronic recorder (3.3.4), set temperature of column chamber, detector, etc.) and record the signal at a sensitivity at least twice as high as that required for the analysis.
Pri tej temperaturi kolono kondicioniramo vsaj dve uri, nato naravnamo instrument v skladu s pogoji delovanja (naravnamo pretok, prižgemo plamen, povežemo z elektronskim rekorderjem (3.3.4), naravnamo delovno temperaturo peči za kolono, naravnamo detektor itd.). Pri občutljivosti, ki je vsaj dvakrat večja od delovne, posnamemo bazno linijo.
The baseline must be linear, with no peaks of any kind, and must not show any deviation.
Le-ta mora biti linearna, brez kakršnih koli vrhov in odklonov.
A negative straight-line drift indicates that the column connections are not tight; a positive drift that the column has not been sufficiently conditioned.
Negativen premočrtni odklon kaže na slabo tesnjenje med kolono in instrumentom, pozitiven odklon pa na slabo kondicionirano kolono.
Choice of operating conditions
Izbira delovnih pogojev
The operating conditions are generally as follows:
Splošni delovni pogoji, ki jih je treba upoštevati, so naslednji:
column temperature:
temperatura kolone:
20 °C/minute
20 °C/minuto
5 °C/minute
5 °C/minuto
Initially 80 °C
na začetku 80 °C
detector temperature: 350 °C;
temperatura detektorja: 350 °C,
quantity of substance injected: 1 μl of the n-heptane solution (2-4 ml);
količina injicirane snovi: 1 μl raztopine (2–4 ml) n-heptana,
carrier gas: helium or hydrogen at the correct linear velocity for the gas selected (see Appendix);
nosilni plin: helij ali vodik z optimalno linearno hitrostjo izbranega plina (glej Dodatek),
instrument sensitivity: suitable for the following conditions:
občutljivost instrumenta: taka, da izpolnjuje spodnje pogoje:
The conditions may be modified according to the characteristics of the column and the GC apparatus to obtain separation of all the waxes and a satisfactory peak resolution (see figure); the internal standard C32 retention time must be 18 ± 3 minutes.
Te pogoje lahko spremenimo glede na značilnosti kolone in plinskega kromografa, da bi dosegli ločevanje vseh voskov, zadostno resolucijo vrhov (glej sliko) in retencijski čas internega standarda C32, ki mora biti 18 ± 3 minute.
The most representative wax peak must be at least 60 % of the full scale.
Najznačilnejši vrh voskov mora biti izmerjen najmanj 60 % od spodnjega dela skale.
The peak integration parameters must be established so as to obtain a correct evaluation of the areas of the peaks in question.
Integracijske parametre moramo določiti tako, da dobimo pravilno vrednotenje površin vrhov.
Given the high final temperature, a positive drift of no more than 10 % of the full scale is permitted.
Opomba: Glede na visoko končno temperaturo je dovoljen pozitivni odklon, ki pa ne sme biti večji od 10 % od spodnjega dela skale.
Performance of the analysis
Izvedba analize
Sample 1 μl of the solution using the 10 μl microsyringe; withdraw the syringe plunger so that the needle is empty.
Z 10-μl mikrobrizgalko odvzamemo 1 μl raztopine; bat izvlečemo, da se igla izprazni.
Place the needle in the injector and after 1-2 seconds inject quickly; remove the needle slowly after about five seconds.
Iglo vbodemo v injektor in po eni ali dveh sekundah hitro vbrizgnemo. Po približno petih sekundah iglo pazljivo izvlečemo.
Record until the waxes are completely eluted.
Kromatogram snemamo, dokler ne eluirajo vsi voski.
The base line must always satisfy the required conditions.
Bazna linija mora ves čas izpolnjevati zahtevane pogoje.
Identification of peaks
Identifikacija vrhov
Identification of the different peaks should be based on retention time by comparison with wax mixtures of known retention times analysed under the same conditions.
Različne vrhove identificiramo na podlagi retencijskih časov in s primerjavo med mešanicami voskov z znanimi retencijskimi časi, ki so bile analizirane pri enakih pogojih.
The figure is a chromatogram of the waxes of a virgin olive oil.
Slika prikazuje kromatogram voskov deviškega oljčnega olja.
Evaluation of quantity
Kvantitativna analiza
Calculate the areas of the peaks of the internal standard and the aliphatic esters of C40 to C46 using the integrator.
Z integratorjem izračunamo površine vrhov alifatskih estrov od C40 do C46 in površino vrha internega standarda.
Calculate the wax content of each of the esters in mg/kg fat using the formula:
Na podlagi naslednje formule izračunamo vsebnost voskov posameznega estra v mg/kg maščobe:
area of each ester’s peak, in square millimetres;
površina vrha posameznega estra, v kvadratnih milimetrih;
area of the internal standard’s peak, in square millimetres;
površina vrha internega standarda, v kvadratnih milimetrih;
mass of added internal standard, in milligrams;
masa dodanega internega standarda, v miligramih;
m mass of sample for analysis, in grams.
m masa odvzetega vzorca za določitev, v gramih.
EXPRESSION OF RESULTS
PODAJANJE REZULTATOV
Indicate the total of the contents of the various C40 to C46 waxes in mg/kg fat (ppm).
Navedemo vsoto vsebnosti posameznih sestavin različnih voskov od C40 do C46, v mg/kg maščobe (ppm).
The components to be quantified refer to the peaks with carbon pair numbers between esters C40 and C46, using the example of the olive oil wax chromatogram shown in the figure below.
Opomba: Spojine, ki jih je treba količinsko določiti, se nanašajo na vrhove s sodim številom ogljikovih atomov med estri C40 do C46, tako kot je to prikazano na kromatograma voskov oljčnega olja.
If ester C46 appears twice, it is recommended that to identify it the fraction of the waxes of an olive-residue oil should be analysed where the C46 peak is easy to identify because it is in the clear majority.
Če sta za ester C46 dva vrhova, je za njegovo identifikacijo priporočljiva analiza frakcije voskov olja iz oljčnih tropin, kjer je vrh C46 dobro viden, ker je razločno večji.
The results should be expressed to one decimal place.
Rezultate je treba izraziti na eno decimalno mesto natančno.
Chromatogram of the waxes of an olive oil
Kromatogram voskov v oljčnem olju
Lauryl arachidate
lauril arahidat
Diterpenic esters
diterpenski estri
2 + 2′ C40 esters
2 + 2′ estri C40
3 + 3′ C42 esters
3 + 3′ estri C42
4 + 4′ Esters C44
4 + 4′ estri C44
esters
estri C46
Sterol esters and triterpenic alcohol
sterolni estri in triterpenski alkoholi.
After elution of the sterol esters the chromatogram trace must not show any significant peaks (triglycerides).
Po eluciji sterolnih estrov kromatografska linija ne sme imeti značilnih vrhov (trigliceridi).
Determination of the linear velocity of the gas
Določanje linearne hitrosti plina
Inject 1-3 μl methane (or propane) into the GC apparatus after it has been regulated to normal operating conditions.
V plinski kromatograf, naravnan na običajne delovne pogoje, injiciramo 1 do 3 μl metana (ali propana).
Measure the time it takes for the gas to flow through the column from the time it is injected to the time the peak appears (tM).
Merimo čas, ki ga plin potrebuje za pot skozi kolono od trenutka vbrizga do trenutka, ko se pojavi vrh (tM).
The linear velocity in cm/s is given by the formula L/tM, where L is the length of the column in cm and tM the time measured in seconds.
Linearna hitrost v cm/s je izražena s formulo L/tM, kjer je L dolžina kolone v cm, tM pa izmerjeni čas v sekundah.
DETERMINATION OF THE PERCENTAGE OF 2-GLYCERYL MONOPALMITATE
DOLOČANJE ODSTOTNEGA DELEŽA 2-GLICERIL MONOPALMITATA
PURPOSE AND SCOPE
PREDMET UREJANJA IN PODROČJE UPORABE
This method describes the analysis procedure for determining the percentage of palmitic acid in position 2 of the triglycerides by evaluating 2-glyceryl monopalmitate.
S to metodo je opisan analitski postopek za določevanje odstotnega deleža palmitinske kisline na položaju 2 v trigliceridih z ovrednotenjem 2-gliceril monopalmitata.
This method can be applied to liquid vegetable oils at ambient temperature (20 °C).
To metodo uporabljamo za rastlinska olja, tekoča pri sobni temperaturi (20 °C).
After preparation the oil sample is subjected to the action of pancreatic lipase: partial and specific hydrolysis in positions 1 and 3 of the triglyceride molecule causes monoglycerides to appear in position 2.
Po pripravi pustimo, da na vzorec olja učinkuje pankreatična lipaza: poteče delna hidroliza, specifična za položaja 1 in 3 v molekuli triglicerida, ki povzroči nastanek 2-monoacilglicerolov.
The percentage of 2-glyceryl monopalmitate in the monoglyceride fraction is determined after silylation by capillary-column gas chromatography.
Odstotni delež 2-gliceril monopalmitata v monoacilglicerolni frakciji se po sililiranju določi s kapilarno plinsko kromatografijo.
APPARATUS AND MATERIALS
APARATURE IN OBIČAJNA LABORATORIJSKA OPREMA
25 ml Erlenmeyer flask
Erlenmajerica, 25 ml