natgillin/translations-raw huggingface dataset:
To improve the pump performance and to obtain a stable baseline, degas the mobile phase daily by selecting one of the available techniques such as sparging by helium, sonication, vacuum or in-line degassing system.
Za izboljšanje delovanja črpalke in doseganje stabilnega izhodišča vsak dan razplinite mobilno fazo s tehniko, ki je dostopna, kot je pršenje s helijem, sonikacija, sistem vakuumskega ali neposrednega razplinjevanja.
Note: In order to prolong column life it is essential that the carbon dioxide content of the eluent is as low as possible and that re-uptake is prevented.
Opomba: Da je kolona uporabna dlje časa, je bistveno, da je vsebnost ogljikovega dioksida v eluentu čim nižja ter da se prepreči ponoven vnos.
Usual laboratory equipment and, in particular, the following:
Običajna laboratorijska oprema in zlasti:
HPLC ion exchange resin column
Kolona HPLC z ionsko izmenjevalno smolo
Column packing: 8 % cross-linked polystyrene-divinylbenzene copolymer functionalised with cation-exchange groups in the lead form.
Polnjenje kolone: 8 % navzkrižno vezanega polistiren-divinilbenzenskega kopolimera, na katerega so vezane skupine za izmenjavo kationov svinca.
Column dimensions: Length 300 mm, internal diameter ca. 8 mm.
Mere kolone: dolžina 300 mm, notranji premer pribl. 8 mm.
Use of other diameters is possible provided that the flow-rate is adjusted accordingly.
Mogoče je uporabiti drugačen premer, ob upoštevanju ustrezno prilagojenega razmerja pretoka.
Guard column
Varovalna kolona
The guard column is a combination of a separate cation exchanger (H+) and an anion exchanger (CO3-), each packed in columns of ca. 30 mm × 4,6 mm (L × ID) (e.g. micro-guard columns in a micro-guard holder) and connected in series or in the form of a mixed bed consisting of AG 50W-X4, -400 mesh (H+) and AG3-X4A, 200–400 mesh (OH-) in the ratio of 35:65 (m/m) manually packed in a column of ca. 20 × 9 mm (L × ID).
Varovalna kolona je kombinacija ločenih izmenjevalnikov kationov (H+) in anionov (CO3–), ki so polnjeni v koloni pribl. 30 mm x 4,6 mm (L x ID) (tj. mikrovarovalne kolone v mikrovarovalnem nosilcu) ter povezani v nizu ali obliki mešane naprave, sestavljene iz AG 50W–X4, – 400 mesh (H+) in AG3-X4A, 200–400 mesh (OH–) v razmerju 35:65 (m/m), ročno polnjeni v koloni pribl. 20 x 9 mm (L x ID).
Column oven
Grelnik kolone
Oven capable of maintaining a constant temperature of 85 °C ± 1 °C.
Grelnik lahko ohranja temperaturo na 85 °C ± 1 °C.
HPLC pump
HPLC-črpalka
Pump capable of generating a constant flow-rate (< 0,5 % fluctuations) at 0,2-1,0 m/min.
Črpalka lahko ohranja stalni pretok (< 0,5 % nihanja) pri 0,2–1,0 m/min.
HPLC injection device
HPLC-vbrizgalna naprava
Auto sampler capable of injecting 25 µL and having a repeatability < 0,5 %.
Avtomatski vzorčevalnik z zmožnostjo vbrizgavanja 25 µL s ponovljivostjo < 0,5 %.
Alternatively a manual device may be used (same requirements as the auto sampler).
Kot druga možnost se lahko uporablja ročna naprava (enake zahteve kot za avtomatski vzorčevalnik).
Highly sensitive refractive index detector having a noise < 5,10-9 RI units.
Visoko občutljiv detektor za refrakcijski indeks, občutljiv do < 5.10–9 RI enot.
Integrator
Integrator
Software or a dedicated integrator to perform data acquisition, processing and generating peak areas and peak heights, which can be converted to lactose concentrations.
Programska oprema ali integrator za pridobivanje in obdelavo podatkov ter prikazovanje površin in višin vrhov, ki se lahko pretvorijo v koncentracije laktoze.
Water purification unit
Enota za čiščenje vode
System capable to provide ultra pure water (type 1) having a resistivity >14 MΩ.cm.
Sistem, ki lahko zagotovi ultra čisto vodo (tip 1) z odpornostjo > 14 MΩ.cm.
Solvent filtration unit
Enota za filtriranje topil
System that enables filtration of the water using 0,45 µm pore size membrane filter.
Sistem, ki omogoča filtracijo vode z uporabo membranskih filtrov z velikostjo por 0,45 µm.
Note: Many water purification units (6.8) have a built in 0,45 or 0,2 µm filtration.
Opomba: Mnoge enote za čiščenje vode (6.8) imajo vgrajene filtre z velikostjo por 0,45 ali 0,2 µm.
Additional filtration can be omitted if this water is directly used.
Dodatnemu filtriranju se lahko izognemo, če se voda neposredno uporabi.
Balance having a read-out of 0,1 mg.
Tehtnica z odčitavanjem 0,1 mg.
Water bath
Vodna kopel
Water bath capable of maintaining a temperature at 40 °C (±0,5).
Vodna kopel, ki lahko temperaturo ohranja na 40 °C (±0,5).
Having a capacity of generating at least 3000 g for Eppendorf vials or equivalent or larger type of vials.
Centrifuga lahko razvije silo najmanj 3000 g z Eppendorfovimi stekleničkami, njihovim ekvivalentom ali večjimi stekleničkami.
Volumetric flask 50 mL
50-mililitrske merilne bučke
Capacity of 50 mL, class A.
Prostornina 50 ml, razred A.
Note: Flasks of other capacity can be used by taking into account the volume factor.
Opomba: Bučke z drugačno prostornino se lahko uporabijo ob upoštevanju volumskega dejavnika.
Volumetric flask 100 mL
100-mililitrske merilne bučke
Capacity of 100 mL, class A.
Prostornina 100 ml, razred A.
Graduated pipette
Graduirana pipeta
Graduated pipette of 10 mL
Graduirana pipeta za 10 ml.
Note: Alternatively, a handpipettor having a capacity of 5 mL can be used by adding twice a volume of 5 mL of reagent (5.3).
Opomba: Kot druga možnost se lahko uporabi ročna pipeta s prostornino 5 ml, tako da dvakrat dodamo 5 ml reagenta (5.3).
It is important that the laboratory receives a sample taken according to ISO 707/IDF 50, which is truly representative and has not been damaged during transport or storage.
Laboratorij mora prejeti vzorec v skladu s standardi ISO 707/IDF 50, ki je resnično reprezentativen in ni bil poškodovan med prevozom ali skladiščenjem.
PREPARATION OF LACTOSE STANDARD SOLUTION
PRIPRAVA STANDARDNE RAZTOPINE LAKTOZE
Standard 1
Standard 1
Dissolve an accurately (read-out at 0,1 mg) weighed amount of ca. 50 mg lactose monohydrate (5.2) in a volumetric flask of 100 mL (6.14) and make up to the mark with water.
Raztopite natančno odtehtano količino (odčitavanje pri 0,1 mg) pribl. 50 mg monohidrata laktoze (5.2) v 100-mililitrski merilni bučki (6.14) in jo dopolnite z vodo do oznake.
Standard 2
Standard 2
Dissolve an accurately (read-out at 0,1 mg) weighed amount of ca. 100 mg lactose monohydrate (5.2) in a volumetric flask of 100 mL (6.14) and make up to the mark with water.
Raztopite natančno odtehtano količino (odčitavanje pri 0,1 mg) pribl. 100 mg monohidrata laktoze (5.2) v 100-mililitrski merilni bučki (6.14) in jo dopolnite z vodo do oznake.
Note: The standard solutions can be stored for maximal 1 week at ca. 5 °C.
Opomba: Standardna raztopina se lahko hrani največ en teden pri pribl. 5 °C.
PREPARATION OF THE TEST SAMPLE
PRIPRAVA PRESKUSNEGA VZORCA
Reconstitution of the sample
Rekonstitucija vzorca
Weigh ca. 5 g of the powder into a flask of 50 mL (6.13) and note the weight at 1 mg accuracy (W1, (11)).
Odtehtajte pribl. 5 g prahu v 50-mililitrsko merilno bučko (6.13) in označite težo z natančnostjo 1 mg (W1, (11)).
Let it subsequently cool to room temperature.
Počakajte, da se ohladi na sobno temperaturo.
Sample treatment
Obdelava vzorca
Take ca. 1 g of this solution and deposit it in a 50 mL volumetric flask (6.13), note the weight at 1 mg accuracy (W3 (11)), add 20 mL of water followed by the addition of 10 mL diluted Biggs/Szijarto reagent (5.4), make up to the mark with water.
Vzemite pribl. 1 g raztopine in jo dajte v 50-mililitrsko merilno bučko (6.13), označite težo z natančnostjo 1 mg (W3 (11)), dodajte 20 ml vode, nato dodajte 10 ml raztopljenega Biggs/Szijartovega reagenta (5.4) in dopolnite z vodo do oznake.
Gently invert the flask 5 times during the first 30 min.
V prvih 30 minutah petkrat nežno obrnite bučko.
After 1 hour take an aliquot and centrifuge (6.12) at 3000 g for 10 min (higher g can be used at correspondingly shorter time).
Po eni uri vzemite alikvotni delež in ga centrifugirajte (6.12) na 3000 g za 10 minut (ob ustreznem krajšem času se lahko uporabi več g).
Use an aliquot of the supernatant for HPLC analysis.
Uporabite alikvotni delež supernatanta za HPLC-analizo.
HPLC DETERMINATION
HPLC-DETEKTOR
Preliminary preparation of HPLC
Predhodna priprava HPLC
Installation of the column and pre-column
Postavitev kolone in predkolone
Install the pre-column (6.2) outside the column oven (6.3) and the column (6.1) in the oven.
Predkolono (6.2) postavite zunaj grelnika kolone (6.3), medtem ko kolono (6.1) postavite v grelnik.
Note: If the oven does not contain tubing to preheat the eluent, it is necessary that the eluent passes ca. 15 cm stainless steel tubing in the oven before entering the column (it is absolutely necessary that the eluent has heated up before entering the column, otherwise peak broadening shall occur).
Opomba: Če grelnik ne vsebuje cevke za ogrevanje eluenta, mora eluent nujno teči skozi približno 15-centimetrsko cevko iz nerjavečega jekla v grelniku, preden pride v kolono (eluent mora biti nujno ogret, preden pride v kolono, v nasprotnem primeru se pojavilo širjenje vrhov).
Detector and initial flow
Detektor in začetni tok
In order to get a stable baseline, turn on the detector (6.6) at least 24 h before starting the analysis.
Da bi dobili stabilno izhodišče, morate detektor (6.6) vključiti najmanj 24 ur pred začetkom analize.
Set the internal temperature of the detector at 35 °C.
Notranjo temperaturo detektorja nastavite na 35 °C.
Set the flow rate at 0,2 ml/min (6.4) for at least 20 min while the column oven (6.3) is set to room temperature.
Pretok nastavite na 0,2 ml/min (6.4) za najmanj 20 minut, medtem ko je grelnik kolone (6.3) nastavljen na sobno temperaturo.
Column oven and final flow-rate
Grelnik kolone in končni pretok
Set the column oven (6.3) at 85 °C.
Grelnik kolone nastavite na (6.3) 85 °C.
When that temperature is reached, increase after 30 minutes gradually the flow rate from 0,2 ml/min to 0,6 ml/min (6.4).
Ko je ta temperatura dosežena, po 30 minutah postopno povečajte pretok z 0,2 ml/min na 0,6 ml/min (6.4).
Allow the system to equilibrate with that flow rate and at 85 °C for 2 h or until a stable baseline is obtained.
S tem pretokom naj sistem deluje 2 uri na 85 °C ali dokler se ne doseže stabilno izhodišče.
Carefully chose the acquisition and integration parameters (6.7) such as data rate, sensitivity, time constant, peak width and threshold.
Previdno izberite parametre za pridobivanje in integracijo (6.7) kot so podatkovna stopnja, občutljivost, časovna konstanta, širina vrhov in pragov.
The retention time of lactose is ca. 11 min.
Retenzijski čas laktoze je pribl. 11 min.
Note: Many data acquisition software programs (6.7) afford easy measurement of the theoretical plate count.
Opomba: Veliko programske opreme za pridobivanje podatkov (6.7) omogoča enostavno merjenje teoretičnega števila mikroorganizmov.
Measure the theoretical plate count of standard 1 (8.1) regularly and replace the column (6.1) when the plate count is 25 % lower than that of the initial value of a new column.
Redno je treba meriti teoretično število mikroorganizmov pri standardu 1 (8.1) in zamenjati kolono (6.1), ko je število mikroorganizmov za 25 % nižje od začetne vrednosti nove kolone.
Guard column test
Preskus varovalne kolone
Check regularly (at least once in every sequence) the ability of the guard column (6.2) to eliminate salts from the sample by injecting 25 µL of a 0,05 % sodium chloride solution.
Redno je treba preveriti (vsaj enkrat na vsako zaporedje) sposobnost varovalne kolone (6.2), da odpravlja soli iz vzorca, z vbrizgavanjem 25 µl 0,05-odstotne raztopine natrijevega klorida.
Whenever peaks are appearing, the guard column should be replaced.
Ko se pojavijo vrhovi, je treba varovalno kolono zamenjati.
Running standards
Standardi delovanja
Inject at the beginning of each series of analyses 25 µL (6.5) of standard 1 (8.1) and subsequently of standard 2 (8.2).
Na začetku vsake vrste analiz je treba vbrizgati 25 µl (6.5) standarda 1 (8.1) in nato standarda 2 (8.2).
Repeat this every 10 to 20 samples and apply this also at the end of the sequence.
Postopek je treba ponoviti na vsakih 10 do 20 vzorcev in na koncu zaporedja.
Running samples
Obravnavani vzorci
Inject 25 µL of the supernatant (9.2) of the sample.
Vbrizgajte 25 µl supernatanta (9.2) vzorca.
CALCULATION AND EXPRESSION OF THE RESULTS
IZRAČUN IN NAVAJANJE REZULTATOV
Normally peak heights are used to calculate the results, however, if the signal contains to much noise peak area can be used (quantitation by peak height is less influenced by peaks of components in low concentration and which are partly, but insufficiently separated from the lactose peak).
Običajno se višine vrhov uporabljajo za izračun rezultatov, vendar če signal vsebuje preveč motenj, uporabimo površino vrhov (na določanje količine z višinami vrhov manj vplivajo vrhovi komponent v manjših koncentracijah in tistih, ki so delno, vendar ne zadostno ločene od vrhov laktoze).
The software (6.7) should calculate a linear calibration curve forced through the origin.
Programska oprema (6.7) mora izračunavati linearno kalibracijsko krivuljo od izhodišča.
Check the curve for possible non linearity (apparent non linearity is most likely caused by a mistake in preparing the standards 1 (8.1) or 2 (8.2), bad integration and, less likely, by a mall functioning injector).
Preverite morebitno nelinearnost krivulje (nelinearnost je verjetno posledica napake pri pripravi standarda 1 (8.1) ali 2 (8.2), slabe vezave in slabo delujočega injektorja, kar je manj verjetno).
Use as input the calculated lactose concentrations in mg/mL of the standards 1 (8.1) and 2 (8.2) as water free lactose.
Kot dovedeno količino uporabite izračunano koncentracijo laktoze v mg/ml standardov 1 (8.1) in 2 (8.2) kot brezvodno laktozo.
The slope (RF) of the calibration line is defined by area/concentration in mg/mL.
Strmina (RF) kalibracijske krivulje je določena s površino/koncentracijo v mg/ml.
Samples
Vzorci Rezultat analize je izražen kot g/100 g in izračunan z uporabo programske opreme (6.7) ali po naslednji formuli: C = H × W1 + W2 × 50 RF × W1 × W3 × 0,1 pri čemer je:
The result of the analysis is obtained as g/100 g and calculated using the software (6.7) or using the following formula:
C koncentracija laktoze v g/100 g prahu H višina vrhov vzorca laktoze
C = H × W1
RF faktor odziva (ali nagib) kalibracijskega grafa v mV/mg/ml W 1 vzorčna masa vzorca prahu v g (9.1) W 2 masa dodane vode vzorcu prahu v g (9.1) W 3
The values derived from this inter laboratory test may not be applicable to concentration ranges and matrices other than those given.
Vrednosti, ki izhajajo iz tega medlaboratorijskega preskusa, mogoče ne bodo ustrezale razponom koncentracij in matricam, ki se od danih razlikujejo.
The values for the repeatability and reproducibility will be derived from the result of an inter laboratory test carried out in accordance with ISO 5725
Vrednosti za ponovljivost in obnovljivost bodo izhajale iz rezultata medlaboratorijskega preskusa v skladu z ISO 5725.
The absolute difference between two single test results, obtained using the same method on identical test material in the same laboratory with the same operator using the same equipment within a short time of interval, will in not more than 5 % of cases be greater than xxx (to be determined by a collaborative trial).
Absolutna razlika med rezultatoma dveh posameznih preskusov, ki sta bila pridobljena po isti metodi na enakem preskusnem materialu v istem laboratoriju pri istem izvajalcu, ki je uporabljal enako opremo, v kratkem časovnem obdobju, bo le v največ 5 % primerov večja od xxx (vrednost bo določena z medlaboratorijskim preskušanjem).
The absolute difference between two single test results, obtained using the same method on identical test material in different laboratories with different operators using different equipment, will in not more than 5 % of cases be greater than 0,5 g/100g (to be determined by a collaborative trial)
Absolutna razlika med rezultatoma dveh posameznih preskusov, ki sta bila pridobljena po enaki metodi na enakem preskusnem materialu v različnih laboratorijih pri različnih izvajalcih, ki so uporabljali različno opremo, bo le v največ 5 % primerov večja od 0,5 g/100 g (vrednost bo določena z medlaboratorijskim preskušanjem).
Koops en C. Olieman, Netherlands Milk and Dairy Journal, 39 (1985) 89-106.
Koops in C. Olieman, Netherlands Milk and Dairy Journal, 39, 1985, 89–106.
ISO 707 (IDF 50), Milk and milk products — Methods of sampling.
ISO 707 (IDF 50), Mleko in mlečni proizvodi – metode vzorčenja.
ISO 5725-1, Accuracy (trueness and precision) of measurement methods and results.
ISO 5725–1, Točnost (pravilnost in natančnost) merilnih metod in rezultatov.
Part 1: General principles and definitions.
Del 1: Splošna načela in definicije.
ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results.
ISO 5725–2, Točnost (pravilnost in natančnost) merilnih metod in rezultatov.
Part 2: A basic method for the determination of repeatability and reproducibility of a standard measurement method.
Del 2: Osnovna metoda za določitev ponovljivosti in obnovljivosti standardne merilne metode.
(Article 9)
(Člen 9)
DETECTION OF RENNET WHEY IN SKIMMED-MILK POWDER FOR PUBLIC STORAGE BY DETERMINATION OF CASEINOMACROPEPTIDES HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
UGOTAVLJANJE SIRIŠČNE SIROTKE V POSNETEM MLEKU V PRAHU ZA JAVNO SKLADIŠČENJE Z DOLOČITVIJO MAKROPEPTIDOV KAZEINA S TEKOČINSKO KROMATOGRAFIJO VISOKE ZMOGLJIVOSTI (HPLC)
This method allows detection of rennet whey in skimmed-milk powder intended for public storage by determination of the caseinomacropeptides.
Ta metoda omogoča zaznavanje siriščne sirotke v posnetem mleku v prahu, ki je namenjeno javnemu skladiščenju z ugotavljanjem makropeptidov kazeina.
International Standard ISO 707 — Milk and Milk Products — Methods of sampling, conforming to the guidelines contained in Annex I(2)I last paragraph.
Mednarodni standard ISO 707 – mleko in mlečni proizvodi – metode vzorčenja, v skladu z navodili iz zadnjega odstavka Priloge I(2)(c).
The content of rennet whey solids is defined as the percentage by mass as determined by the caseinomacropeptide content by the procedure described.
Vsebnost trdnih snovi siriščne sirotke je opredeljena kot masni odstotek, ki se določi z vsebnostjo makropeptidov kazeina v skladu z opisanim postopkom.
Reconstitution of the skimmed-milk powder, removal of fat and proteins with trichloroacetic acid, followed by centrifugation or filtration.
Rekonstitucija posnetega mleka v prahu, odstranitev maščob in beljakovin s trikloroocetno kislino, ki ji sledi centrifugacija ali filtracija.
Determination of the quantity of caseinomacropeptides (CMP) in the supernatant by high-performance liquid chromatography (HPLC).
Določitev količine makropeptidov kazeina (CMP) v supernatantu se izvede s tekočinsko kromatografijo visoke ločljivosti (HPLC).
Evaluation of the result obtained for the samples by reference to standard samples consisting of skimmed-milk powder with or without the addition of a known percentage of whey powder.
Ovrednotenje rezultatov, dobljenih za vzorce, glede na standardne vzorce posnetega mleka v prahu z dodatkom znanega odstotka sirotke v prahu ali brez njega.
Trichloroacetic acid solution
Raztopina trikloroocetne kisline
Dissolve 240 g of trichloroacetic acid (CCl3COOH) in water and make up to 1000 ml.
Raztopite 240 g trikloroocetne kisline (CCl3CCOOH) v vodi in dopolnite do prostornine 1000 ml.
The solution should be clear and colourless.
Raztopina mora biti bistra in brezbarvna.
Eluent solution, pH 6,0
Raztopina eluenta, pH 6,0
Dissolve 1,74 g of dipotassium hydrogen phosphate (K2HPO4), 12,37 g of potassium dihydrogen phosphate (KH2PO4) and 21,41 g of sodium sulphate (Na2SO4) in about 700 ml of water.
Raztopite 1,74 g dikalijevega hidrogenfosfata (K2HPO4), 12,37 g monokalijevega fosfata (KH2PO4) in 21,41 g natrijevega sulfata (Na2SO4) v približno 700 ml vode.
Adjust, if necessary, to pH 6,0, using a solution of phosphoric acid or potassium hydroxide.
Če je potrebno, uravnajte pH na vrednost 6,0 z uporabo raztopin fosforjeve kisline ali kalijevega hidroksida.
Make up to 1000 ml with water and homogenise.
Dopolnite z vodo do 1000 ml in homogenizirajte.
Note: The composition of the eluent can be updated to comply with the certificate of the standards or the recommendations of the manufacturer of the column packing material.
Opomba: Sestava eluenta se lahko posodobi tako, da ustreza spričevalu v skladu s standardi ali priporočili proizvajalca materiala za polnitev kolone.
Filter the eluent solution, prior to use, through a membrane filter with a 0,45 μm pore diameter.
Raztopino topila pred uporabo filtrirajte skozi membranski filter, ki ima premer por 0,45 μm.
Flushing solvent
Raztopina za izpiranje
Mix one volume acetonitrile (CH3CN) with nine volumes water.
Zmešajte eno volumsko enoto acetonitrila (CH3CN) z devetimi volumskimi enotami vode.
Filter the mixture prior to use through a membrane filter with a 0,45 μm pore diameter.
Pred uporabo filtrirajte zmes z membranskimi filtrom s premerom por 0,45 μm.
Note: Any other flushing solvent with a bactericidal effect which does not impair the columns’ resolution efficiency may be used.
Opomba: Uporabi se lahko katera koli druga raztopina za izpiranje z baktericidnim učinkom, ki ne poslabša ločljivosti kolone.
Standard samples
Standardni vzorci
Skimmed-milk powder meeting the requirements of this Resolution (i.e. [0])
Posneto mleko v prahu, ki ustreza zahtevam te resolucije (npr. [0])
The same skimmed-milk powder adulterated with 5 % (m/m) rennet-type whey powder of standard composition (i.e. [5]).
Enako posneto mleko v prahu z dodatkom 5 % (m/m) siriščne sirotke v prahu s standardno sestavo (tj. [5]).
Optional centrifuge capable of attaining a centrifugal force of 2200 g, fitted with stoppered or capped centrifuge tubes of about 50 ml capacity
Neobvezna centrifuga, ki lahko razvije centrifugalno silo 2200 g, z epruvetami za centrifugiranje z zamaški ali pokrovčki s prostornino približno 50 ml
Mechanical shaker
Mehanski stresalnik
Glass funnels, diameter about 7 cm
Stekleni lijaki s premerom približno 7 cm
Filter papers, medium filtration, diameter about 12,5 cm
Filtrirni papir, srednje porozen, s premerom približno 12,5 cm
Glass filtration equipment with 0,45 μm pore diameter membrane filter
Oprema za filtriranje iz stekla s premerom por membranskega filtra 0,45 μm
Graduated pipettes allowing delivery of 10 ml (ISO 648, Class A, or ISO/R 835) or a dispensing system capable of delivering 10,0 ml in two minutes
Graduirana pipeta, ki prepušča 10 ml (ISO 648, razred A, ali ISO/R 835) ali razdelilni sistem, ki lahko prepušča 10,0 ml v dveh minutah
Dispensing system capable of delivering 20,0 ml water at ca. 50 °C
Razdelilni sistem, ki lahko prepušča 20,0 ml vode pri pribl. 50 °C
Thermostatic water bath, set at 25 ± 0,5 °C
Termostatska vodna kopel, nastavljena na 25 ± 0,5 °C
HPLC equipment, consisting of:
HPLC-oprema, ki jo sestavljajo:
Pump
Črpalka
Injector, hand or automatic, with a 15 to 30 μl capacity
Injektor, ročni ali avtomatski, s prostornino 15 do 30 μl
Two TSK 2000-SW columns in series (length 30 cm, internal diameter 0,75 cm) or equivalent columns (e.g. single TSK 2000-SWxl, single Agilent Technologies Zorbax GF 250) and a precolumn (3 cm × 0,3 cm) packed with I 125 or material of equivalent effectiveness
Dve serijski koloni TSK 2000-SW (dolžina 30 cm, notranji premer 0,75 cm) ali istovrstni koloni (tj. enojna TSK 2000-SWxl, enojna Agilent Technologies Zorbax GF 250) in predkolona (3 cm × 0,3 cm), polnjena z I 125 ali enako učinkovitim materialom
Thermostatic column oven, set at 35 ± 1 °C
Termostatiran grelnik kolone, nastavljen na 35 ± 1 °C
Variable wavelength UV detector, permitting measurements at 205 nm with a sensitivity of 0,008 A
UV-detektor z nastavljivo valovno dolžino, ki omogoča meritve pri 205 nm z občutljivostjo 0,008 A
Integrator capable of valley-to-valley integration
Integrator, ki lahko poveže doline
Note: Working with columns kept at room temperature is possible, but their power of resolution is slightly lower.
Opomba: Mogoče je delo s kolonami pri sobni temperaturi, vendar je njihova moč resolucije nekoliko manjša.