natgillin/translations-raw huggingface dataset:
The results should be interpreted with caution where measured toxicant concentrations in test solutions occur at levels near the detection limit of the analytical method.
Rezultate je treba razlagati previdno, kadar so merjene koncentracije toksičnih snovi v preskusnih raztopinah blizu meje detekcije analitske metode.
The interpretation of results for concentrations above the water solubility of the substance should also be made with care.
Prav tako pazljivo je treba razložiti rezultate za koncentracije nad topnostjo snovi v vodi.
THE TEST REPORT
POROČILO O PRESKUSU
chemical identification data, including purity and analytical method for quantification of the tests substance where appropriate.
identifikacijski podatki o kemikaliji, vključno s čistostjo in analitsko metodo za količinsko določanje preskusne snovi, kjer je to ustrezno.
scientific name, strain, numbers of parental fish (i.e. how many females were used for providing the required numbers of eggs in the test), source and method of collection of the fertilised eggs and subsequent handling.
znanstveno ime, sev, število starševskih rib (t.j. koliko samic je bilo uporabljenih za zagotovitev potrebnega števila iker v preskusu), vir in metoda zbiranja oplojenih iker in nadaljnje ravnanje z njimi.
test procedure used (e.g. semi-static or flow-through, time period from fertilisation to start the test, loading, etc),
uporabljeni preskusni postopek (npr. semi-statični ali pretočni, časovno obdobje od oploditve do začetka preskusa, obremenitev itn.),
photoperiod(s),
fotoperioda,
test design (e.g. number of test chambers and replicates, number of embryos per replicate),
načrt preskusa (npr. število preskusnih komor in ponovitev, število embriev na ponovitev),
the nominal test concentrations, the measured values, their means and their standard deviations in the test vessels and the method by which these were attained and, if the test substance is soluble in water at concentrations below those tested, evidence should be provided that the measurements refer to the concentrations of the test substance in solution,
nominalne preskusne koncentracije, izmerjene vrednosti, njihove srednje vrednosti in standardni odkloni v preskusnih posodah in metoda določitve, ter če je preskusna snov topna v vodi v koncentracijah pod preskušenimi, je treba priskrbeti dokaze, da se meritve nanašajo na koncentracije preskusne snovi v raztopini,
dilution water characteristics: pH, hardness, temperature, dissolved oxygen concentration, residual chlorine levels (if measured), total organic carbon, suspended solids, salinity of the test medium (if measured) and any other measurements made,
značilnosti vode za redčenje: pH, trdota, temperatura, koncentracija raztopljenega kisika, koncentracija sledi klora (če so bile merjene), skupni organski ogljik, suspendirani trdni delci, slanost preskusnega medija (če je bila merjena) in katere koli druge izvedene meritve,
water quality within test vessels: pH, hardness, temperature and dissolved oxygen concentration.
kakovost vode v preskusnih posodah: pH, trdota, temperatura in koncentracija raztopljenega kisika.
results from any preliminary studies on the stability of the test substance,
rezultati kakršnih koli uvodnih študij stabilnosti preskusne snovi,
evidence that controls met the overall survival acceptability standard of the test species (Appendices 2 and 3),
dokazi, da kontrole ustrezajo standardom sprejemljivega preživetja za to preskusno vrsto (Dodatka 2 in 3),
data on mortality/survival at embryo and larval stages and overall mortality/survival,
podatki o smrtnosti/preživetju v embrionalnem stadiju in stadiju ličink ter skupna smrtnost/preživetje,
days to hatch and numbers hatched,
dnevi do izvalitve in število izvaljenih ličink,
data for length (and weight),
podatki o dolžini (in teži),
incidence and description of morphological abnormalities, if any,
pojav in opis morebitnih morfoloških nepravilnosti,
incidence and description of behavioural effects, if any,
pojav in opis morebitnih učinkov na vedenje,
statistical analysis and treatment of data,
statistična analiza in obdelava podatkov,
for tests analysed using ANOVA, the lowest observed effect concentration (LOEC) at p=0,05 and the no observed effect concentration (NOEC) for each response assessed, including a description of the statistical procedures used and an indication of what size of effect could be detected,
za preskuse, analizirane z metodo ANOVA, ocena najnižje koncentracije z opaznim učinkom (LOEC) pri p = 0,05 in koncentracije brez opaznih učinkov (NOEC) za vsak obravnavan odziv, vključno z opisom uporabljenih statističnih postopkov in obseg opaženega učinka,
for tests analysed using regression techniques, the LC/ECx and confidence intervals and a graph of the fitted model used for its calculation,
za preskuse, analizirane z regresijskimi tehnikami, LC/ECx in intervali zaupanja ter graf prilagojenega modela („graf of fitted model“), uporabljenega za izračun,
explanation for any deviation from this testing method.
pojasnilo morebitnih odstopanj od te preskusne metode.
Kristensen P., (1990) Evaluation of the Sensitivity of Short Term Fish Early Life Stage Tests in Relation to other FELS Test Methods.
Kristensen P. (1990) Evaluation of the Sensitivity of Short Term Fish Early Life Stage Tests in Relation to other FELS Test Methods.
Final report to the Commission of the European Communities, p. 60.
Final report to the Commission of the European Communities, str 60.
E 1241-88. p. 26
Str. 26.
Brauhn J.L. and Schoettger R.A., (1975) Acquisition and Culture of Research Fish: Rainbow trout, Fathead minnows, Channel Catfish and Bluegills. p. 54, Ecological Research Series, EPA-660/3-75-011, Duluth, Minnesota.
Brauhn J.L. and Schoettger R.A. (1975). Acquisition and Culture of Research Fish: Rainbow trout, Fathead minnows, Channel Catfish and Bluegills. p.54, Ecological Research Series, EPA-660/3-75-011, Duluth, Minnesota.
Brungs W.A. and Jones B.R., (1977) Temperature Criteria for Freshwater Fish: Protocol and Procedures p. 128, Ecological Research Series EPA-600/3-77-061, Duluth, Minnesota.
Brungs W.A. and Jones B.R. (1977) Temperature Criteria for Freshwater Fish: Protocol and Procedures p. 128, Ecological Research Series EPA-600/3-77-061, Duluth, Minnesota.
Laale H.W., (1977) The Biology and Use of the Zebrafish (Brachydanio rerio) in Fisheries Research.
Laale H.W. (1977) The Biology and Use of the Zebrafish (Brachydanio rerio) in Fisheries Research.
Legault R. (1958) A Technique for Controlling the Time of Daily Spawning and Collecting Eggs of the Zebrafish, Brachydanio rerio (Hamilton-Buchanan) Copeia, 4, p. 328-330.
Legault R. (1958) A Technique for Controlling the Time of Daily Spawning and Collecting Eggs of the Zebrafish, Brachydanio rerio (Hamilton-Buchanan) Copeia, 4, str. 328–330.
Dave G., Damgaard B., Grande M., Martelin J.E., Rosander B. and Viktor T., (1987) Ring Test of an Embryo-larval Toxicity Test with Zebrafish (Brachydanio rerio) Using Chromium and Zinc as Toxicants.
Dave G., Damgaard B., Grande M., Martelin J.E., Rosander B. and Viktor T. (1987). Ring Test of an Embryo-larval Toxicity Test with Zebrafish Brachydanio rerio) Using Chromium and Zinc as Toxicants.
Environmental Toxicology and Chemistry, 6, p. 61-71.
Environmental Toxicology and Chemistry , 6, str. 61–71.
Birge J.W., Black J.A. and Westerman A.G., (1985) Short-term Fish and Amphibian Embryo-larval Tests for Determining the Effects of Toxicant Stress on Early Life Stages and Estimating Chronic Values for Single Compounds and Complex Effluents.
Birge J.W., Black J.A. and Westerman A.G. (1985). Short-term Fish and Amphibian Embryo-larval Tests for Determining the Effects of Toxicant Stress on Early Life Stages and Estimating Chronic Values for Single Compounds and Complex Effluents.
Environmental Toxicology and Chemistry 4, p. 807-821.
Environmental Toxicology and Chemistry 4, str. 807–821.
Aquatic Toxicology, 9, p. 129-145.
Aquatic Toxicology, 9, 129–145.
Carolina Biological Supply Company, North Carolina. p. 36.
Str. 36.
Dunnett C.W., (1955) A Multiple Comparisons Procedure for Comparing Several Treatments with Control.
Dunnett C.W. (1955) A Multiple Comparisons Procedure for Comparing Several Treatments with Control.
Mc Clave J.T., Sullivan J.H. and Pearson J.G., (1980) Statistical Analysis of Fish Chronic Toxicity Test Data.
Mc Clave J.T., Sullivan J.H. and Pearson J.G. (1980).
Van Leeuwen C.J., Adema D.M.M. and Hermes J., (1990) Quantitative Structure-Activity Relationships for Fish Early Life Stage Toxicity.
Van Leeuwen C.J., Adema D.M.M. and Hermes J. (1990). Quantitative Structure-Activity Relationships for Fish Early Life Stage Toxicity.
Aquatic Toxicology, 16, p. 321-334.
Aquatic Toxicology, 16, str. 321–334.
Report EPS 1/RM/28, December 1992, p. 81.
Report EPS l/RM/28, December 1992, str. 81.
Arch. of Environmental Contamination and Toxicology, 21, p. 126-134.
Arch. of Environmental Contamination and Toxicology, 21, 126–134.
Meyer A., Bierman C.H. and Orti G., (1993) The phylogenetic position of the Zebrafish (Danio rerio), a model system in developmental biology — an invitation to the comperative methods.
Meyer A., Bierman C.H. and Orti G. (1993). The phylogenetic position of the Zebrafish (Danio rerio), a model system in developmental biology – an invitation to the comperative methods.
Royal Society of London, Series B, 252: 231-236.
Royal Society of London, Series B, 252: 231–236.
Ecotoxicology and Environmental Safety 32, p. 19-28.
Ecotoxicology and Environmental Safety 32, 19–28.
De Graeve G.M., Cooney J.D., McIntyre D.O., Poccocic T.L., Reichenbach N.G., Dean J.H. and Marcus M.D., (1991) Validity in the performance of the seven-day Fathead minnow (Pimephales promelas) larval survival and growth test: an intra- and interlaboratory study.
De Graeve G.M., Cooney J.D., Mclntyre DO., Poccocic T.L., Reichenbach N.G., Dean J.H. and Marcus M.D. (1991).
Tox.
Environ. Tox.
Balon E.K., (1985) Early life history of fishes: New developmental, ecological and evolutionary perspectives, Junk Publ., Dordrecht, p. 280.
Balon E.K. (1985). Early life history of fishes: New developmental, ecological and evolutionary perspectives, Junk Publ., Dordrecht, str. 280.
Blaxter J.H.S., (1988) Pattern and variety in development, In: W.S.
Blaxter J.H.S. (1988).
Hoar and D.J.
Pattern and variety in development, In: W.S.
Randall Eds., Fish Physiology, vol. XIA, Academic press, p. 1-58.
Hoar and D.J. Randall Eds., Fish Physiology, vol. XIA, Academic press, str. 1–58.
Table 1A
Tabela 1A
FRESHWATER
SLADKOVODNE
Rainbow trout (9)(16)
Šarenka (9) (16)
Zebrafish (7)(17)(18)
Cebrica (7) (17) (18)
Common carp (8)(19)
Krap (8) (19)
Fathead minnow (8)(22)
Črnoglavi pisanec (8) (22)
Table 1B
Tabela 1B
Examples of other well-documented species which have also been used
Primeri drugih dobro dokumentiranih vrst, ki so bile tudi uporabljene
SALTWATER
MORSKE
Tidewater silverside (23)(24)(25)
Bibavični glavun (23) (24) (25)
Sheepshead minnow (23)(24)(25)
Bisernasti krapič (23) (24) (25)
GUIDANCE ON PERFORMANCE OF A TOXICITY TEST ON EMBRYOS AND SAC-FRY OF ZEBRAFISH (BRACHYDANIO RERIO)
SMERNICE ZA IZVEDBO PRESKUSA TOKSIČNOSTI NA EMBRIIH IN MLADICAH S HRANILNIM MEŠIČKOM CEBRICE (Brachydanio rerio)
The zebrafish originates from the Coromandel coast of India where it inhabits fast-flowing streams.
Cebrica izvira iz Koromandijske obale Indije, kjer naseljuje hitro tekoče vode.
It is a common aquarium fish of the carp family, and information about procedures for its care and culture can be found in standard reference books on tropical fish.
To je pogosta akvarijska riba iz družine krapov, tako da lahko podatke o njeni negi in gojenju dobimo v standardni referenčni literaturi o tropskih ribah.
Its biology and use in fishery research have been reviewed by Laale (1).
Njeno biologijo in uporabo v ribogojniških raziskavah, je podal Laale (1).
The fish rarely exceeds 45 mm in length.
Ta riba redko doseže dolžino več kot 45 mm.
The body is cylindrical with 7-9 dark-blue horizontal silvery stripes.
Njeno telo je cilindrične oblike s 7–9 temno modrimi vodoravnimi srebrnkastimi progami.
These stripes run into the caudal and anal fins.
Proge se stekajo v repno in predrepno plavut.
The back is olive-green.
Hrbet je olivnozelen.
Males are slimmer than females.
Samci so vitkejši od samic.
Females are more silvery and the abdomen is distended, particularly prior to spawning.
Samice so bolj srebrnkaste in njihov trebuh je napihnjen, zlasti pred drstenjem.
Adult fishes are able to tolerate large fluctuations in temperature, pH and hardness.
Odrasle ribe lahko prenesejo velika nihanja v temperaturi, pH in trdoti vode.
However, in order to get healthy fish which produce eggs of good quality, optimal conditions should be provided.
Vendar je treba zagotoviti optimalne pogoje, da bi dobili zdrave ribe, ki proizvajajo kvalitetne ikre.
During spawning the male pursues and butts the female, and as the eggs are expelled they are fertilised.
Med drstenjem samec zasleduje samico in se zaletava vanjo, in ko samica izloča ikre, jih samec oplaja.
The eggs, which are transparent and non-adhesive, fall to the bottom where they may be eaten by the parents.
Ikre, ki so prozorne in nelepljive, padejo na dno, kjer jih starši včasih pojedo.
Spawning is influenced by light.
Na drstenje vpliva svetloba.
If the morning light is adequate, the fish usually spawns in the early hours following daybreak.
Če je jutranja svetloba primerna, se ribe navadno drstijo v zgodnjih jutranjih urah takoj po zori.
A female can produce batches of several hundreds of eggs at weekly intervals.
Samica lahko proizvede serije več sto iker v tedenskih razmikih.
CONDITIONS OF PARENTAL FISH, REPRODUCTION AND EARLY-LIFE STAGES
POGOJI ZA STARŠEVSKE RIBE. REPRODUKCIJO IN ZGODNJE RAZVOJNE STADIJE
Select a suitable number of healthy fish and keep these in suitable water (e.g. Annex 4) for at least two weeks prior to the intended spawning.
Izberite primerno število zdravih rib in jih vsaj dva tedna pred nameravanim drstenjem hranite v ustrezni vodi (npr. Dodatek 4).
The group of fish should be allowed to breed at least once before producing the batch of eggs used in the test.
Skupini rib je treba omogočiti, da se razmnožujejo vsaj enkrat, preden naredijo serijo iker, ki bo uporabljena v preskusu.
The density of fish during this period should not exceed 1 gram of fish per litre.
Gostota rib v tem času ne sme preseči 1 gram rib na liter.
Regular changes of water or the use of purification systems will enable the density to be higher.
Redno menjavanje vode ali uporaba prečiščevalnih sistemov bosta omogočila večjo gostoto.
The temperature in the holding tanks should be maintained at 25 ± 2 oC.
V bazenih je treba ohranjati temperaturo 25 ± 2 oC.
A number of ‘spawning-trees’, consisting of untwisted nylon rope, are attached to the coarse net of the frame.
Na grobo mrežo na okvirju pritrdimo več „drstnih debel“, ki so iz ravne najlonske vrvice.
The spawning tray will prevent the fish from eating the eggs and at the same time permit an easy collection of the eggs.
Drstitveni pladenj bo ribam onemogočil, da bi pojedle ikre, hkrati pa omogočil, da se jih brez težav pobere.
The group of fish should have spawned at least once before the spawning from which eggs are used for testing.
Skupina rib se mora drstiti vsaj enkrat pred tistim drstenjem, katerega ikre so uporabljene za preskušanje.
Five to 10 male and female fish are housed individually at least two weeks prior to the intended spawning.
Pet do deset ribjih samcev in samic nastanimo posamič vsaj dva tedna pred načrtovanim drstenjem.
After 5-10 days, the abdomens of the females will be distended and their genital papillae visible.
Po 5–10 dneh se trebuhi samic napihnejo in njihove genitalne papile postanejo vidne.
The water temperature is gradually increased one degree higher than the acclimatisation temperature.
Postopno povečujemo temperaturo vode do ene stopinje nad prilagoditveno temperaturo.
The light is turned off and the tank is left as undisturbed as possible.
Ugasnemo svetilko in pustimo bazen čim bolj pri miru.
If larger batches of eggs are needed than can be obtained from one female, a sufficient number of spawning tanks may be set-up in parallel.
Če potrebujemo večje skupke iker, kot jih lahko dobimo od ene samice, lahko hkrati postavimo dovolj veliko število drstnih bazenov.
By recording the reproduction success of the individual females prior to the test (size of batch and quality), those females with highest reproduction success may be selected for breeding.
Če si zapisujemo produktivnost posameznih samic pred preskusom (velikost in kakovost skupka), lahko izberemo tiste, ki so najbolj uspešne pri reprodukciji.
The eggs should be transferred to the test vessels by means of glass tubes (inner diameter not less than 4 mm) provided with a flexible suction bulb.
Ikre moramo prenesti v preskusne posode s steklenimi cevkami (z notranjim premerom, ki ni manjši od 4 mm), ki imajo namestljivo sesalno bučo.
The amount of water accompanying the eggs on their transfer should be as small as possible.
Količina vode pri prenosu iker mora biti čim manjša.
The eggs are heavier than water and sink out of the tube.
Ikre so težje od vode in se odtočijo iz cevke.
Care should be taken to prevent eggs (and larvae) coming into contact with the air.
Paziti moramo, da ikre (in ličinke) ne bi prišle v stik z zrakom.
Also the development rate and the rate of hatching vary from one batch to another.
Serije se razlikujejo tudi v hitrosti razvoja in stopnja izvalitve.
The successfully fertilised eggs and the yolk sac larvae survive well, normally above 90 %.
Preživetje uspešno oplojenih iker in ličink s hranilnim mešičkom je dobro, večinoma 90 %.
At 25 oC the eggs will hatch three-five days after fertilisation and the yolk sac will be absorbed approximately 13 days after fertilization.
Pri 25 oC se ličinke izležejo 3–5 dni po oploditvi in absorbirajo hranilni mešiček približno 13 dni po oploditvi.
The embryonic development has been well defined by Hisaoka and Battle (2).
Embrionalni razvoj sta dobro opisala Hisaoka in Battle (2).
Due to the transparency of the eggs and post-hatch larvae, the development of the fish may be followed and the presence of malformations may be observed.
Zaradi prosojnosti iker in ličink po izvalitvi lahko sledimo razvoju ribe in opazimo prisotnost deformnosti.
Approximately four hours after spawning, the non-fertilized eggs may be distinguished from the fertilized (3).
Približno štiri ure po drstenju lahko ločimo neoplojene ikre od oplojenih (3).
For this examination, eggs and larvae are placed in test vessels of small volume and studied under a microscope.
Za ta pregled položimo ikre v preskusne posode majhne prostornine in jih proučujemo pod mikroskopom.
The test conditions, which apply to the early life stages, are listed in Appendix 2.
Preskusni pogoji, ki se nanašajo na zgodnje razvojne stadije, so našteti v Dodatku 2.
Then, for those concentrations at which no adverse effects on any of these parameters have been detected, the body length is statistically evaluated.
Nato se statistično ovrednoti dolžina telesa pri tistih koncentracijah, pri katerih ne opazimo škodljivih učinkov na katerega koli od teh parametrov.
This approach is advisable since the toxicant may selectively kill smaller fish, delay hatching-time and induce gross malformations, thus leading to biased length measurements.
Ta pristop je priporočljiv, ker lahko toksična snov selektivno ubija manjše ribe, odloži čas izvalitve in povzroča velike deformnosti in so zato meritve dolžine lahko pristranske.
Furthermore, there will be roughly the same number of fish to be measured per treatment, ensuring the validity of the test statistics.
Poleg je treba pri vsakem tretiranju izmeriti približno enako število rib, da zagotovimo veljavnost preskusne statistike.
Therefore multiple comparison procedures should be utilised (6)(7)(8)(9)(10).
Zato moramo uporabiti postopke multiple primerjave (6) (7) (8) (9) (10).
Hisaoka K.K. and Battle H.I., (1958).
Hisaoka K.K. and Battle H.I. (1958).
The Normal Development Stages of the Zebrafish Brachydanio rerio (Hamilton-Buchanan) J. Morph., 102, p. 311.
The Normal Development Stages of the Zebrafish Brachydanio rerio (Hamilton-Buchanan) J. Morph., 102, str. 311.
Journal of Applied Ichthyology, 2, p. 173-181.
Journal of Applied Ichthyology, 2, str. 173–181.
Finney D.J., (1971).
Finney D.J. (1971).
Probit Analysis, 3rd ed., Cambridge University Press, Great Britain, p. 1-333.
Probit Analysis, 3rd ed., Cambridge University Press, Great Britain, str. 1–333.
Stephan C.E., (1982).
Stephan C.E. (1982).
Bishop, Eds., American Society for Testing and Materials, p. 69-81.
Bishop, Eds., American Society for Testing and Materials, str. 69–81.
Dunnett C.W., (1964) New Tables for Multiple Comparisons with a Control.
Dunnett C.W. (1964) New Tables for Multiple Comparisons with a Control.
Williams D.A., (1971).
Williams D.A. (1971).
Biometrics, 27, p. 103-117.
Biometrics, 27, str. 103–117.
Williams D.A., (1972).
Williams D.A. (1972).
Biometrics 28, p. 519-531.
Biometrics 28, str. 519–531.
Sokal R.R. and Rohlf F.J., (1981).
Sokal R.R. and Rohlf F.J. (1981).
TEST CONDITIONS, DURATION AND SURVIVAL CRITERIA FOR RECOMMENDED SPECIES
PRESKUSNI POGOJI, TRAJANJE IN MERILA PREŽIVETJA ZA PRIPOROČENE VRSTE
Salinity
SLANOST
Photo-period
FOTO-PERIODA
Duration of stages
TRAJANJE STADIJEV
Typical duration of test
TIPIČNO TRAJANJE PRESKUSA
Survival of control,
PREŽIVETJE V KONTROLAH,
(minimum %)
(NAJMANJŠI %)
Hatching success
uspeh izvalitve
Post-hatch
po izvalitvi
As soon as possible after fertilisation (early gastrula stage) to 5 days post-hatch (8-10 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 5 dni po izvalitvi (8–10 dni)
Rainbow trout
šarenka
As soon as possible after fertilisation (early gastrula stage) to 20 days post-hatch (50-55 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 20 dni po izvalitvi (50–55 dni)
As soon as possible after fertilisation (early gastrula stage) to 4 days post-hatch (8-9 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 4 dni po izvalitvi (8–9 dni)
As soon as possible after fertilisation (early gastrula stage) to 5 days post-hatch (13-16 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 5 dni po izvalitvi (13–16 dni)
Fathead minnow
črnoglavi pisanec
For embryos.
Za embrie.
For larvae.
Za ličinke.
Darkness for embryo and larvae until one week after hatching except when they are being inspected.
Tema ze embrie in ličinke, dokler ne mine en teden po izvalitvi, razen kadar se jih pregleduje.
Then subdued lighting throughout the test.
Nato blaga svetloba skozi celoten preskus
Test conditions, duration and survival criteria for other well documented species
Preskusni pogoji, trajanje in merila preživetja za druge dobro dokumentirane vrste
Salinity (0/00)
SLANOST (0/00)
Photo-period (hrs)
FOTOPERIODA (ure)
Duration of stages (days)
TRAJANJE STADIJEV (dnevi)
Typical duration of embryo and sac-fry test
TIPIČNO TRAJANJE PRESKUSA
Survival of control (minimum %)
PREŽIVETJE V KONTROLAH, (NAJMANJŠI %)
Sac-fry test
MLADICA S HRANILNIM MEŠIČKOM
Goldfish
zlata ribica
As soon as possible after fertilisation (early gastrula stage) to 4 days post-hatch (7 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 4 dni po izvalitvi (7 dni)
Blugill sunfish
vrsta ostriža
Tidewater silverside
bibavični glavun
As soon as possible after fertilisation (early gastrula stage) to 5 days post-hatch (6-7 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 5 dni po izvalitvi (6–7 dni)
As soon as possible after fertilisation (early gastrula stage) to 3 days post-hatch (23-27 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 3 dni po izvalitvi (23–27 dni)
As soon as possible after fertilisation (early gastrula stage) to 3 days post-hatch (18 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 3 dni po izvalitvi (18 dni)
As soon as possible after fertilisation (early gastrula stage) to 4/7 days post-hatch (28 days)
Čim prej po oploditvi (zgodnji stadij gastrule) do 4/7 dni po izvalitvi (28 dni)
This acute toxicity test method is a replicate of the OECD TG 213 (1998).
Ta preskusna metoda akutne toksičnosti ustreza metodi OECD TG 213 (1998).