natgillin/translations-raw huggingface dataset:
(notified under document number C(2008) 7642)
(notificirano pod dokumentarno številko C(2008) 7642)
Having regard to Council Directive 64/432/EEC of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine [1], and in particular Article 6(2)(b) and the second subparagraph of Article 16 thereof,
ob upoštevanju Direktive Sveta 64/432/EGS z dne 26. junija 1964 o problemih v zvezi z zdravstvenim varstvom živali, ki vplivajo na trgovino z govedom in prašiči znotraj Skupnosti [1], ter zlasti člena 6(2)(b) in drugega pododstavka člena 16(1) Direktive,
Annex C to Directive 64/432/EEC sets out the diagnostic methods for bovine brucellosis to be used for the control and eradication of that disease and for surveillance and monitoring, as well as for the establishment and maintenance of an officially brucellosis–free herd status and certification required for intra-Community trade in bovine animals.
Priloga C k Direktivi Sveta 64/432/EGS določa diagnostične metode za govejo brucelozo za uporabo pri kontroli in izkoreninjenju navedene bolezni ter nadzoru in spremljanju, kakor tudi za ugotovitev in vzdrževanje statusa črede, uradno proste bruceloze, ter za zahteve certificiranja za trgovino z govedom znotraj Skupnosti.
Commission Decision 2004/226/EC of 4 March 2004 approving tests for the detection of antibodies against bovine brucellosis within the framework of Council Directive 64/432/EEC [2] approves certain tests for bovine brucellosis that may be used as an alternative to the mandatory serum agglutination test (SAT) for certification of bovine animals in accordance with Article 6(2)(b) of Directive 64/432/EEC.
V Odločbi Komisije 2004/226/ES z dne 4. marca 2004 o odobritvi testov za odkrivanje protiteles proti goveji brucelozi v okviru Direktive Sveta 64/432/EGS [2] so odobreni nekateri testi za govejo brucelozo, ki se lahko uporabijo kot druga možnost za obvezen seroaglutinacijski test (SAT) za certificiranja goveda v skladu s členom 6(2)(b) Direktive 64/432/EGS.
The fluorescence polarisation assay (FPA) is a new diagnostic test that has been included as a prescribed test for international trade in Chapter 2.4.3 (bovine brucellosis) of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008 of the World Organisation for Animal Health (OIE).
Test s fluorescentnimi označevalci (FPA) je novi diagnostični test, ki je bil vključen kot predpisan test za mednarodno trgovino v poglavju 2.4.3 (goveja bruceloza) Priročnika za diagnostične teste in cepiva za kopenske živali, šesta izdaja, 2008 Svetovne organizacije za zdravje živali (OIE).
The Commission requested the European Food Safety Authority (EFSA) to provide a scientific opinion on the suitability of the FPA for inclusion in Annex C to Directive 64/432/EEC.
Komisija je Evropsko agencijo za varnost hrane (EFSA) zaprosila za znanstveno mnenje o ustreznosti FPA za vključitev v Prilogo C k Direktivi 64/432/EGS.
In addition, the Commission asked EFSA to assess the suitability of the FPA and the tests listed in Article 1 of Decision 2004/226/EC for the purpose of certification of bovine animals for intra-Community trade.
Poleg tega je Komisija agencijo EFSA zaprosila, naj oceni ustreznost FPA in teste, navedene v členu 1 Odločbe 2004/226/ES, za namen certificiranja goveda za trgovino znotraj Skupnosti.
On 11 December 2006, the Panel on animal health and welfare adopted a scientific opinion on brucellosis diagnostic methods for bovines [3], in which it concluded that, except the SAT, the diagnostic tests for bovine brucellosis, included in Annex C to Directive 64/432/EEC are suitable to remain as standard tests for the purpose of certification of individual bovine animals for intra-Community trade.
Komisija za zdravje in dobro počutje živali je 11. decembra 2006 sprejela znanstveno mnenje o diagnostičnih metodah za govejo brucelozo [3], v katerem je ugotovila, da razen SAT, diagnostični testi za govejo brucelozo, vključeni v Prilogo C k Direktivi 64/432/EGS, še naprej ustrezajo kot standardni testi za namen certificiranja posameznega goveda za trgovino znotraj Skupnosti.
However, as the SAT is the pre-movement test for trade in cattle directly prescribed in Article 6(2)(b) of Directive 64/432/EEC, a technical specification must be available in Annex C to that Directive.
Ker pa je SAT za trgovino z govedom pred premikom izrecno predpisan v členu 6(2)(b) Direktive 64/432/EGS, mora biti tehnična specifikacija na voljo v Prilogi C k navedeni direktivi.
In addition, the scientific opinion of 11 December 2006 concluded that the sensitivity and specificity of the FPA are comparable to those of tests included in Annex C to Directive 64/432/EEC and was also found to be suitable for inclusion in that Annex as a standard test for brucellosis diagnosis in such animals for intra-Community trade.
Poleg tega je bilo v znanstvenem mnenju z dne 11. decembra 2006 ugotovljeno, da je FPA, kar zadeva občutljivost in specifičnost, primerljiv s testi, vključenimi v Prilogo C k Direktivi 64/432/EGS, in je bil ugotovljen za ustreznega za vključitev v navedeno prilogo kot standardni test za diagnozo bruceloze pri takšnih živalih za trgovino znotraj Skupnosti.
The recently developed polymerase chain reaction methods as described in Section 1(d) of Chapter 2.4.3 of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008 of the OIE provide additional means of detection and identification of Brucella spp. and should therefore be included in Annex C to Directive 64/432/EEC.
Nedavno razvite metode verižne reakcije s polimerazo, kot so opisane v oddelku 1(d) poglavja 2.4.3 Priročnika za diagnostične teste in cepiva za kopenske živali OIE, šesta izdaja, 2008, zagotavljajo dodatne preskuse za odkrivanje in identifikacijo Brucella spp. in jih je zato treba vključiti v Prilogo C k Direktivi 64/432/EGS.
Annex C to Directive 64/432/EEC and Decision 2004/226/EC should therefore be amended accordingly.
Prilogo C k Direktivi 64/432/EGS in Odločbo 2004/226/ES je zato treba ustrezno spremeniti.
Annex C to Directive 64/432/EEC is replaced by the text in the Annex to this Decision.
Priloga C k Direktivi 64/432/EGS se nadomesti z besedilom v Prilogi k tej odločbi.
Article 1 of Decision 2004/226/EC is replaced by the following:
Člen 1 Odločbe 2004/226/ES se nadomesti z naslednjim:
The complement fixation test, the buffered Brucella antigen test (rose Bengal test (RBT)), the ELISA tests and the fluorescence polarisation assay (FPA) carried out in accordance with Annex C to Directive 64/432/EEC are hereby approved for certification purposes.’
Test reakcije vezanja komplementa, Brucella antigen test v pufru (rose bengal test (RBT), ELISA testi in test s fluorescentnimi označevalci (FPA), opravljeni v skladu z določbami Priloge C k Direktivi 64/432/EGS, se odobrijo za namene certificiranja.“
Done at Brussels, 10 December 2008.
V Bruslju, 10. decembra 2008
OJ L 68, 6.3.2004, p. 36.
UL L 68, 6.3.2004, str. 36.
Annex C to Directive 64/432/EEC is replaced by the following:
Točke 1, 2 in 3 Priloge C k Direktivi 64/432/EGS se nadomestijo z naslednjim:
IDENTIFICATION OF THE AGENT
DOLOČITEV POVZROČITELJA
The demonstration by modified acid-fast or immunospecific staining of organisms of Brucella morphology in abortion material, vaginal discharges or milk provides presumptive evidence of brucellosis, especially if supported by serological tests.
Dokaz mikroorganizmov z morfologijo brucel z modificiranim barvanjem za acidorezistentne bakterije ali imunskospecifičnim barvanjem v zavrženem materialu, vaginalnih izcedkih ali mleku daje dokaz za domnevo bruceloze, zlasti če je podprt s serološkimi testi.
The polymerase chain reaction (PCR) methods provide additional means of detection.
Metode verižne reakcije s polimerazo (PCR) zagotavljajo dodatne preskuse za odkrivanje.
Whenever possible, Brucella spp. should be isolated using plain or selective media by culture from uterine discharges, aborted foetuses, udder secretions or selected tissues, such as lymph nodes and male and female reproductive organs.
Kadar koli je možno, je treba Brucella spp. prek kulture iz vaginalnih izcedkov, zavrženih zarodkov, izcedkov iz vimena ali izbranih tkiv, kot so bezgavke ter moški in ženski reproduktivni organi, osamiti z uporabo običajnih ali selektivnih gojišč.
After isolation, the species and biovar shall be identified by phage lysis and/or oxidative metabolism tests, cultural, biochemical and serological criteria.
Po osamitvi se določita vrsta in biovar z lizo z bakteriofagom in/ali testi oksidativnega metabolizma, gojiščno preiskavo ter biokemičnimi in serološkimi merili.
PCR can provide both a complementary and biotyping method based on specific genomic sequences.
PCR lahko s pomočjo posebnih genomskih sekvenc služi kot dopolnilna metoda in kot metoda ribotipizacije.
The techniques and media used, their standardisation and the interpretation of results must conform to that specified in the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008, Chapter 2.4.3 (bovine brucellosis), Chapter 2.7.2 (caprine and ovine brucellosis) and Chapter 2.8.5 (porcine brucellosis).
Uporabljene tehnike in sredstva, njihova standardizacija in razlaga rezultatov morajo biti v skladu s tistimi, opredeljenimi v Priročniku za diagnostične teste in cepiva za kopenske živali OIE, šesta izdaja, 2008, poglavje 2.4.3. (goveja bruceloza), poglavje 2.7.2 (ovčja in kozja bruceloza) in poglavje 2.8.5 (prašičja bruceloza).
IMMUNOLOGICAL TESTS
IMUNOLOŠKI TESTI
The Brucella abortus biovar 1 Weybridge strain No 99 or USDA strain 1119-3 must be used for the preparation of all antigens used in the rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT) and the milk ring test (MRT).
Za pripravo vseh antigenov, uporabljanih v rose bengal testu (RBT), seroaglutinacijskem testu (SAT), reakciji vezanja komplementa (RVK) in mlečnem prstanastem preskusu (MPP), se mora uporabljati Brucella abortus biovar 1 Weybridge sev št. 99 ali USDA sev 1119-3.
The standard reference serum for the RBT, SAT, CFT and MRT shall be the OIE international reference standard serum (OIEISS) formerly named WHO second international anti-Brucella abortus Serum (ISAbS).
Standardni referenčni serum za RBT, SAT, RVK in MPP je mednarodni standardni referenčni serum OIE (OIEISS), prej imenovan drugi mednarodni standardni serum proti Brucella abortus WHO (ISAbS).
The standard reference sera for enzyme-linked immunosorbent assays (ELISAs) shall be:
Standardni referenčni serumi za encimskoimunske teste (teste ELISA) so:
the weak positive OIE ELISA standard serum (OIEELISAWPSS),
šibek pozitivni OIE ELISA standardni serum (OIEELISAWPSS),
the strong positive OIE ELISA standard serum (OIEELISASPSS),
močan pozitivni OIE ELISA standardni serum (OIEELISASPSS),
the negative OIE ELISA standard serum (OIEELISANSS).
negativni OIE EISA standardni serum (OIEELISANSS).
The standard reference sera for fluorescence polarisation assays (FPAs) shall be:
Standardni referenčni serumi za teste s fluorescentnimi označevalci (teste FPA) so:
The standard sera listed in 2.1.3 and 2.1.4 are available from the Community reference laboratory for brucellosis or the Veterinary Laboratories Agency (VLA), Weybridge, United Kingdom.
Standardni serumi, navedeni v 2.1.3 in 2.1.4, so na voljo pri Referenčnem laboratoriju Skupnosti za brucelozo ali Veterinary Laboratories Agency (VLA), Weybridge, Združeno kraljestvo.
The OIEISS, the OIEELISAWPSS, the OIEELISASPSS and the OIEELISANSS are international primary standards from which secondary reference national standards serum (working standards) must be established for each test referred to in 2.1.1 in each Member State.
OIEISS, OIEELISAWPSS, OIEELISASPSS in OIEELISANSS so primarni mednarodni standardi, iz katerih se morajo določiti sekundarni nacionalni referenčni standardi (‚delovni standardi‘) za vsak test iz 2.1.1 v vsaki državi članici.
Enzyme-linked immunosorbent assays (ELISAs) or other binding assays for the detection of bovine brucellosis in serum or milk
Encimskoimunski testi (ELISA testi) ali drugi testi z markiranimi protitelesi za odkrivanje goveje bruceloze v serumu ali mleku
Material and reagents
Material in reagenti
The technique used and the interpretation of results must have been validated in accordance with the principles laid down in Chapter 1.1.4 of the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Sixth Edition, 2008, and must include at least laboratory and diagnostic studies.
Uporabljena tehnika in razlaga rezultatov morata biti validirani v skladu z načeli iz poglavja 1.1.4 Priročnika za diagnostične teste in cepiva za kopenske živali OIE, šesta izdaja, 2008, in morata vključevati najmanj laboratorijske in diagnostične študije.
Standardisation of the test
Standardizacija testa
Standardisation of the test procedure for individual serum samples:
Standardizacija postopka testa za posamezne vzorce seruma:
a 1/150 pre-dilution [1] of the OIEISS or a 1/2 pre-dilution of the OIEELISAWPSS or a 1/16 pre-dilution of the OIEELISASPSS made up in a negative serum (or in a negative pool of sera) must give a positive reaction;
predrazredčina 1/150 [1] OIEISS, predrazredčina 1/2 OIEELISAWPSS ali predrazredčina 1/16 OIEELISASPSS morajo v negativnem serumu (ali negativnem skupinskem vzorcu serumov) dati pozitivno reakcijo;
a 1/600 pre-dilution of the OIEISS or a 1/8 pre-dilution of the OIEELISAWPSS or a 1/64 pre-dilution of the OIEELISASPSS made up in a negative serum (or in a negative pool of sera) must give a negative reaction;
predrazredčina 1/600 OIEISS, predrazredčina 1/8 OIEELISAWPSS ali predrazredčina 1/64 OIEELISASPSS morajo v negativnem serumu (ali negativnem skupinskem vzorcu serumov) dati negativno reakcijo;
the OIEELISANSS must always give a negative reaction.
OIEELISANSS mora vedno dati negativno reakcijo.
Standardisation of the test procedure for pooled serum samples:
Standardizacija postopka testa za skupinske vzorce serumov:
a 1/150 pre-dilution of the OIEISS or a 1/2 pre-dilution of the OIEELISAWPSS or a 1/16 pre-dilution of the OIEELISASPSS made up in a negative serum (or in a negative pool of sera) and again diluted in negative sera by the number of samples making up the pool must give a positive reaction;
predrazredčina 1/150 OIEISS, predrazredčina 1/2 OIEELISAWPSS ali predrazredčina 1/16 OIEELISASPSS morajo v negativnem serumu (ali negativnem skupinskem vzorcu serumov) in ponovno razredčene v negativnih serumih s številom vzorcev, ki sestavljajo skupinski vzorec, dati pozitivno reakcijo;
the OIEELISANSS must always give a negative reaction;
OIEELISANSS mora vedno dati negativno reakcijo;
the test must be adequate to detect evidence of infection in a single animal of the group of animals, of which samples of serum have been pooled.
test mora zadostovati za odkrivanje okužbe ene same živali v skupini živali, iz katere so zbrani vzorci seruma v skupinski vzorec.
Standardisation of the test procedure for pooled milk or whey samples:
Standardizacija postopka testa za skupinske vzorce mleka ali sirotke:
a 1/1000 pre-dilution of the OIEISS or a 1/16 pre-dilution of the OIEELISAWPSS or a 1/125 pre-dilution of the OIEELISASPSS made up in a negative serum (or in a negative pool of sera) and again diluted 1/10 in negative milk must give a positive reaction;
predrazredčina 1/1000 OIEISS, predrazredčina 1/16 OIEELISAWPSS ali predrazredčina 1/125 OIEELISASPSS morajo v negativnem serumu (ali negativnem skupnem vzorcu serumov) in ponovno razredčene 1/10 v negativnem mleku dati pozitivno reakcijo;
the OIEELISANSS diluted 1/10 in negative milk must always give a negative reaction;
OIEELISANSS, razredčen 1/10 v negativnem mleku, mora vedno dati negativno reakcijo;
the test must be adequate to detect evidence of infection in a single animal of the group of animals, of which samples of milk or whey have been pooled.
test mora zadostovati za odkrivanje okužbe ene same živali v skupini živali, iz katere so zbrani vzorci mleka ali sirotke za skupinski vzorec.
Conditions for use of the ELISAs for diagnosis of bovine brucellosis:
Pogoji za uporabo ELISA testov za diagnozo goveje bruceloze:
Using the calibrating conditions for ELISAs set out in point 2.2.2.1 and 2.2.2.2 on serum samples, the diagnostic sensitivity of ELISA shall be equal or greater than the RBT or CFT taking into account the epidemiological situation under which it is employed.
Z uporabo pogojev za kalibracijo ELISA testov vzorcev seruma iz točk 2.2.2.1 in 2.2.2.2 je diagnostična občutljivost ELISA testa enaka ali večja kot občutljivost RBT ali RVK, ob upoštevanju epidemiološke situacije, pri kateri je uporabljen.
Using the calibrating conditions for ELISA set out in point 2.2.2.3 on pooled milk samples, the diagnostic sensitivity of ELISA shall be equal or greater than the MRT taking into account not only the epidemiological situation but also the average and expected extreme husbandry systems.
Z uporabo pogojev za kalibracijo ELISA testa skupinskih vzorcev mleka iz točke 2.2.2.3 je diagnostična občutljivost ELISA testa enaka ali večja kot občutljivost MPP, ob upoštevanju ne samo epidemiološke situacije, temveč tudi povprečnih in pričakovanih ekstremnih sistemov reje.
Where ELISAs are used for certification purposes in accordance with Article 6(1) or for the establishment and maintenance of a herd status in accordance with Annex A(II)(10), pooling of samples of serum must be carried out in such a way that the test results can be undoubtedly related to the individual animal included in the pool.
Če se ELISA testi uporabljajo za certificiranje v skladu s členom 6(1) ali za ugotavljanje in vzdrževanje statusa črede v skladu s Prilogo A(II)(10), je treba zbiranje vzorcev seruma v skupinski vzorec izvesti tako, da bodo rezultati testa nesporno povezani s posamezno živaljo, vključeno v skupinski vzorec.
Any confirmatory test must be carried out on samples of serum taken from individual animals.
Kateri koli test za potrditev se mora opraviti na vzorcih seruma, odvzetih posameznim živalim.
The ELISAs may be used on a sample of milk taken from the milk collected from a farm with at least 30 % of dairy cows in milk.
ELISA testi se lahko uporabljajo na vzorcu mleka iz mleka, ki ga odda kmetija in v katerem je zajetih vsaj 30 % krav mlekaric, ki niso presušene.
If that method is used, measures must be taken to ensure that the samples taken for examination can be undoubtedly related to the individual animals from which the milk derived.
Če se uporablja navedena metoda, je treba z ukrepi zagotoviti, da je mogoče preiskovane vzorce nesporno povezati s posameznimi živalmi, od katerih je mleko pridobljeno.
Complement fixation test (CFT)
Reakcija vezanja komplementa (RVK)
The antigen represents a bacterial suspension in phenol-saline (NaCl 0,85 % (m/v) and phenol at 0,5 % (v/v)) or in a veronal buffer.
Antigen je suspenzija bakterij v fiziološki raztopini s fenolom (koncentracija NaCl 0,85 % in volumski delež fenola 0,5 %) ali v veronalnem pufru.
Antigens may be delivered in the concentrated state provided the dilution factor to be used is indicated on the bottle label.
Koncentrirane antigene se lahko dobavi, če je na etiketi stekleničke naveden faktor razredčenja, ki ga je treba uporabiti.
The antigen must be stored at 4 °C and not frozen.
Antigen je treba hraniti na 4 °C in ne zamrznjen.
Serums must be inactivated as follows:
Serume je treba inaktivirati na naslednji način:
bovine serum: 56 to 60 °C for 30 to 50 minutes,
goveji serum: 56 do 60 °C za 30 do 50 minut,
porcine serum: 60 °C for 30 to 50 minutes.
prašičji serum: 60 °C za 30 do 50 minut.
In order to carry out the genuine reaction within the test procedure, a complement dose higher than the minimum necessary for total haemolysis shall be used.
Da bi se lahko v postopku testa izvedla prava reakcija, se uporabi doza komplementa, ki je višja od minimalno potrebne za popolno hemolizo.
In carrying out the complement fixation test, the following controls must be made each time:
Pri izvajanju reakcije vezanja komplementa je treba vedno opraviti naslednje kontrole:
control of the anti-complementary effect of the serum;
kontrola antikomplementarnega delovanja seruma;
control of the antigen;
kontrola antigena;
control of sensitised red blood cells;
kontrola senzibiliziranih eritrocitov;
control of the complement;
kontrola komplementa;
control using a positive serum of sensitivity at the start of the reaction;
kontrola občutljivosti s pozitivnim serumom na začetku reakcije;
control of the specificity of the reaction using a negative serum.
kontrola specifičnosti reakcije z negativnim serumom.
The OIEISS contains 1000 international CFT units (ICFTU) per ml.
OIEISS vsebuje 1000 internacionalnih RVK enot (ICFTU) na mililiter.
If the OIEISS is tested in a given method the result is given as a titre (i.e. highest direct dilution of the OIEISS giving 50 % haemolysis, TOIEISS).
Če se v dani metodi testira OIEISS, se rezultat poda kot titer (tj. višje neposredno razredčenje OIEISS da 50 % hemolizo, TOIEISS).
The test result for the test serum given as titre (TTESTSERUM) must be expressed in ICFTU per ml.
Rezultat testa za testirani serum se poda kot titer (TTESTSERUM) in mora biti podan v ICFTU na mililiter.
In order to convert the expression of a titre into ICFTU, the factor (F) necessary to convert a titre of an unknown test serum (TTESTSERUM) tested by that method into the ICFTU expression can be found from the formula:
Za pretvorbo vrednosti titra v ICFTU se lahko faktor (F), potreben za pretvorbo titra neznanega, s to metodo testiranega seruma (TTESTSERUM) v ICFTU določi s formulo:
and the content of international CFT units per ml of test serum (ICFTUTESTSERUM) from the formula:
in vsebnost internacionalnih RVK enot na ml testiranega seruma (ICFTUTESTSERUM) s formulo:
Interpretation of results
Razlaga rezultatov
A serum containing 20 or more ICFTU per ml shall be considered to be positive.
Serum, ki vsebuje 20 ali več ICFTU na ml, se pojmuje kot pozitiven.
Milk ring test (MRT)
Mlečni prstanasti preskus (MPP)
The antigen represents a bacterial suspension in phenol-saline (NaCl 0,85 % (m/v) and phenol at 0,5 % (v/v)) stained with haematoxylin.
Antigen je suspenzija bakterij v fiziološki raztopini s fenolom (koncentracija NaCl 0,85 % in volumski delež fenola 0,5 %), obarvana s hematoksilinom.
The antigen sensitivity must be standardised in relation to the OIEISS in such a way that the antigen produces a positive reaction with a 1/500 dilution of the OIEISS in negative milk, while a 1/1000 dilution must be negative.
Občutljivost antigena je treba standardizirati glede na OIEISS, tako da antigen povzroči pozitivno reakcijo z razredčino 1/500 OIEISS v negativnem mleku, medtem ko mora biti razredčina 1/1000 negativna.
The ring test must be made on samples representing the contents of each milk churn or the content of each bulk tank from the farm.
Prstanasti preskus je treba opraviti na vzorcih, ki so reprezentativni za vsebino vsake posode za mleko ali večjega rezervoarja s kmetije.
The milk samples must not have been frozen, heated or subjected to violent shaking.
Vzorcev mleka se ne sme zamrzniti, greti ali močno stresati.
The reaction must be carried out using one of the following methods:
Reakcija mora biti opravljena z uporabo ene od naslednjih metod:
on a column of milk of at least 25 mm height and on a volume of milk of 1 ml to which either 0,03 ml or 0,05 ml of one of the standardised stained antigens has been added,
s stolpcem mleka višine vsaj 25 mm in volumnom mleka 1 ml, čemur se doda 0,03 ali 0,05 ml enega izmed standardiziranih obarvanih antigenov,
on a column of milk of at least 25 mm height and on a volume of milk of 2 ml to which 0,05 ml of one of the standardised stained antigens has been added,
s stolpcem mleka višine vsaj 25 mm in z volumnom mleka 2 ml, čemur se doda 0,05 ml enega izmed standardiziranih obarvanih antigenov,
on a volume of milk of 8 ml to which 0,08 ml of one of the standardised stained antigens has been added.
z volumnom mleka 8 ml, čemur se doda 0,08 ml enega izmed standardiziranih obarvanih antigenov.