natgillin/translations-raw huggingface dataset:
Wilting symptoms are very different from those of brown rot; wilting is usually slow and initially limited to the leaf margins.
Simptomi venenja so precej različni od simptomov rjave gnilobe; venenje je običajno počasno in na začetku omejeno na robove listov.
Young infected leaves often continue to expand although, less so in the infected zones.
Mladi okuženi listi se še naprej razvijajo, čeprav manj na okuženih območjih.
This creates odd shaped leaves.
To povzroča čudno oblikovane liste.
Leaves affected by blocking of the vascular tissues further down the stem often develop chlorotic, yellow to orange, intercostal areas.
Listi, prizadeti z blokado vaskularnega tkiva na spodnjem delu stebla, imajo pogosto klorotične, rumeno oranžne vmesne dele.
Infected leaflets, leaves and even stems may eventually die.
Okuženi lističi, listi in celo stebla lahko sčasoma odmrejo.
Often leaves and tubers are simply reduced in size.
Pogosto so listi in gomolji samo manjše velikosti.
Occasionally plants are stunted.
Včasih so rastline okrnjene.
Coloured pictures of a range of symptoms can be found on the web site http://forum.europa.eu.int/Public/irc/sanco/Home/main
Barvne slike razpona simptomov so dostopne na spletni strani: http://forum.europa.eu.int/Public/irc/sanco/Home/main
Potato tubers
Gomolji krompirja
The earliest symptoms are a slight glassiness or translucence of the tissue without softening around the vascular system, particularly near the heel end.
Prvi simptomi so rahlo steklast videz ali prosojnost tkiva brez omehčanja okoli vaskularnega sistema, predvsem v bližini stolona.
The vascular ring at the heel end may be slightly darker in colour than normal.
Vaskularni obroček na stolonu je lahko rahlo temnejše barve kot ponavadi.
The first readily identifiable symptom is one whereby the vascular ring has a yellowish coloration and when the tuber is gently squeezed, pillars of cheese-like material emerge from the vessels.
Prvi lahko prepoznavni simptom predstavlja rumenkasto obarvan vaskularni obroček in če gomolj rahlo stisnemo, se iz žil pocedijo majhne količine siraste mase.
This exudation contains millions of bacteria.
Ta izcedek vsebuje na milijone bakterij.
Browning of the vascular tissue may develop and tuber symptoms at this stage are similar to those of brown rot caused by Ralstonia solanacearum.
V tej fazi se lahko vaskularno tkivo obarva rjavo in simptomi gomolja so podobni simptomom rjave gnilobe, ki jo povzroča Ralstonia solanacearum.
At first, these symptoms may be restricted to one part of the ring, not necessarily close to the heel end and may gradually extend to the whole ring.
Ti simptomi so lahko sprva omejeni le na del obročka, ki ni nujno v bližini stolona, in se lahko postopoma razširijo na celoten obroček.
As the infection progresses, destruction of the vascular tissue occurs; the outer cortex may become separated from the inner cortex.
Z napredovanjem okužbe se pojavi uničenje vaskularnega tkiva; zunanji korteks se lahko oddvoji od notranjega korteksa.
In advanced stages of infection, cracks appear on the surface of the tuber, which are often reddish-brown at their margins.
V poznejših fazah okužbe se na površini gomolja pojavijo razpoke, ki so ob robu pogosto rdečkastorjave barve.
Recently in Europe several cases have occurred where the central cortex rots at the same time as the vascular ring resulting in secondary invasion with internal hollowing and necrosis.
V zadnjem času se je v Evropi pojavilo nekaj primerov, ko je osrednji korteks gnil istočasno kot vaskularni obroček, kar je pripeljelo do sekundarnega napada z notranjim votlenjem in nekrozo.
Secondary fungal or bacterial invasion may mask the symptoms and it may be difficult, if not impossible, to distinguish advanced ring rot symptoms from other tuber rots.
Sekundarni napad gliv ali bakterij lahko prikrije simptome in tako oteži ali onemogoči razločevanje simptomov obročkaste gnilobe v poznem stadiju od ostalih gnilob gomoljev.
Atypical symptoms may be possible.
Možni so neznačilni simptomi.
SAMPLE PREPARATION
PRIPRAVA VZORCEV
The standard sample size is 200 tubers per test.
Standardna velikost vzorca je 200 gomoljev na test.
More intensive sampling requires more tests on samples of this size.
Intenzivnejše vzorčenje zahteva več testov na vzorcih te velikosti.
Larger numbers of tubers in the sample will lead to inhibition or difficult interpretation of the results.
Večje število gomoljev v vzorcu bo povzročilo inhibicijo ali otežilo interpretacijo rezultatov.
However, the procedure can be conveniently applied for samples with less than 200 tubers where fewer tubers are available.
Vendar pa se postopek lahko ustrezno uporabi za vzorce z manj kot 200 gomolji, če je na voljo manj gomoljev.
Validation of all detection methods described below is based on testing of samples of 200 tubers.
Potrditev vseh spodaj opisanih metod odkrivanja temelji na testiranju vzorca z 200 gomolji.
The potato extract described below can also be used for detection of the potato brown rot bacterium, Ralstonia solanacearum.
Spodaj opisani krompirjev ekstrakt se lahko uporabi tudi za odkrivanje bakterije krompirjeve rjave gnilobe, Ralstonia solanacearum.
Optional pre-treatment in advance to sample preparation:
Izbirna vnaprejšnja predobdelava pred pripravo vzorca:
Wash the tubers.
Operemo gomolje.
Use appropriate disinfectants (chlorine compounds when PCR-test is to be used in order to remove eventual pathogen DNA) and detergents between each sample.
Uporabimo ustrezna razkužila (klorove spojine, kadar se uporabi test PCR, da se odstrani morebitno patogeno DNK) in detergente med vsakim vzorcem.
Air dry the tubers.
Gomolje posušimo na zraku.
This washing procedure is particularly useful (but not required) for samples with excess soil and if a PCR-test or direct isolation procedure is to be performed.
Ta postopek pranja je zlasti koristen (vendar ni obvezen) pri vzorcih z odvečno zemljo in če se bosta izvedla test PCR ali postopek neposredne izolacije.
Remove with a clean and disinfected scalpel or vegetable knife the skin at the heel end of each tuber so that the vascular tissue becomes visible.
S čistim in razkuženim skalpelom ali nožem za zelenjavo odstranimo povrhnjico na območju stolona vsakega gomolja, tako da vaskularno tkivo postane vidno.
Carefully cut out a small core of vascular tissue at the heel end and keep the amount of non-vascular tissue to a minimum (see web site: http://forum.europa.eu.int/Public/irc/sanco/Home/main)
Previdno izrežemo majhno jedro vaskularnega tkiva na območju stolona in pazimo, da je nevaskularnega tkiva čim manj (glej spletno stran: http://forum.europa.eu.int/Public/irc/sanco/Home/main)
Set aside any tubers with suspected ring rot symptoms and test separately.
Ločimo vse gomolje s sumljivimi simptomi obročkaste gnilobe in posebej testiramo.
If during removal of the heel end core suspect symptoms of ring rot are observed, the tuber should be visually inspected after cutting near the heel end.
Če med odstranitvijo jedra stolona opazimo sumljive simptome obročkaste gnilobe, je treba ta gomolj prerezati blizu stolona in ga vizualno pregledati.
Any cut tuber with suspected symptoms should be suberised at room temperature for two days and stored under quarantine (at 4 to 10 °C) until all tests have been completed.
Vsak prerezan gomolj s sumljivimi simptomi je treba suberizirati na sobni temperaturi dva dni in nato shraniti v karanteni (pri 4 do 10 °C), dokler niso vsi testi končani.
All tubers in the sample (including those with suspicious symptoms) should be kept according to Annex II.
Vsi gomolji v vzorcu, vključno tisti s sumljivimi simptomi, se shranjujejo v skladu s Prilogo II.
Process the heel end cores by one of the following procedures: either,
Jedra stolonov obdelamo po enem od naslednjih postopkov:
cover the cores with sufficient volume (approximately 40 ml) of extraction buffer (Appendix 3) and agitate on a rotary shaker (50 to 100 rpm) for four hours below 24 °C or for 16 to 24 hours refrigerated,
Prekrijemo jedra z zadostno količino (približno 40 ml) ekstrakcijskega pufra (Dodatek 3) in pretresemo na rotacijskem stresalniku (50-100 vrt/min) 4 ure pri temperaturi pod 24 °C in 16 do 24 ur pri temperaturi hladilnika,
homogenise the cores with sufficient volume (approximately 40 ml) of extraction buffer (Appendix 3), either in a blender (e.g. Waring or Ultra Thurax) or by crushing in a sealed disposable maceration bag (e.g. Stomacher or Bioreba strong guage polythene, 150 mm × 250 mm; radiation sterilised) using a rubber mallet or suitable grinding apparatus (e.g. Homex).
homogeniziramo jedra z zadostno količino (približno 40 ml) ekstrakcijskega pufra (Dodatek 3), bodisi v mešalcu (npr. Waring ali Ultra Thurax) ali pa jih zmeljemo v zaprti meceracijski vrečki za enkratno uporabo (npr. močan tesnilni politen Stomacher ali Bioreba, 150 mm × 250 mm; steriliziran z obsevanjem), pri čemer uporabimo gumijast bat ali ustrezno aparaturo za mletje (npr. Homex).
The risk of cross-contamination of samples is high when samples are homogenized using a blender.
Če se vzorci homogenizirajo z mešalcem, je tveganje navzkrižne okužbe vzorcev veliko.
Take precautions to avoid aerosol generation or spillage during the extraction process.
Z ustreznimi ukrepi se ognemo nastajanju pršenja ali razlitju med postopkom ekstrakcije.
Ensure that freshly sterilised blender blades and vessels are used for each sample.
Za vsak vzorec moramo uporabiti sveže razkužena rezila in posodo mešalca.
If the PCR test is to be used, avoid carry-over of DNA on containers or grinding apparatus.
Če uporabljamo test PCR, se moramo izogibati prenosu DNK na posodah ali aparaturah za mletje.
Crushing in disposable bags and use of disposable tubes is recommended where PCR is to be used.
Mletje v vrečkah za enkratno uporabo in uporaba cevk za enkratno uporabo se priporoča pri testu PCR.
Decant the supernatant.
Odlijemo supernatant.
If excessively cloudy, clarify either by slow speed centrifugation (at not more than 180 g for 10 minutes at a temperature between 4 to 10 °C) or by vacuum filtration (40 to 100 µm), washing the filter with additional (10 ml) extraction buffer (Appendix 3).
V primeru prevelike motnosti, sčistimo s počasnim centifugiranjem (ne več kot 180 g za 10 min pri temperaturi 4 do 10 °C) ali z vakumsko filtracijo (40 do 100 µm), pri čemer filter operemo z dodatnim (10 ml) ekstrakcijskim pufrom (Dodatek 3).
Concentrate the bacterial fraction by centrifugation at 7000 g for 15 minutes (or 10000 g for 10 minutes) at a temperature between 4 to 10 °C and discard the supernatant without disturbing the pellet.
Skoncentriramo bakterijsko frakcijo s centrifugiranjem na 7000 g 15 minut (ali 10000 g 10 minut) pri temperaturi 4 do 10 °C in odlijemo supernatant brez premešavanja pelete.
Resuspend the pellet in 1,5 ml pellet buffer (Appendix 3).
Resuspendiramo peleto v 1,5 ml peletnega pufra (Dodatek 3).
Use 500 µl to test for C. m. subsp. sepedonicus, 500 µl for Ralstonia solanacearum and 500 µl for reference purposes.
Uporabimo 500 µl za testiranje C. m. subsp. sepedonicus, 500 µl za Ralstonia solanacearum in 500 µl za referenčne namene.
Add sterile glycerol to final concentration of 10 to 25 % (v/v) to the 500 µl of the reference aliquot and to the remaining test aliquot, vortex and store at –16 to –24 °C (weeks) or at 68 to –86 °C (months).
Dodamo sterilni glicerol končni koncentraciji 10-25 % (v/v) 500 µl referenčnega alikvota in preostanku testnega alikvota, premešamo z vrtinčenjem in shranimo pri –16 do –24 °C (tedni) ali pri –68 do –86° C (meseci).
Preserve the test aliquots at 4 to 10 °C during testing.
Testne alikvote hranimo pri 4 do 10 °C med testiranjem.
Repeated freezing and thawing is not advisable.
Večkratno zamrzovanje in tajanje ni priporočljivo.
If transport of the extract is required, ensure delivery in a cool box within 24 to 48 hours.
Če je potreben transport ekstrakta, zagotovimo dostavo v hladilni skrinji v 24-ih do 48-ih urah.
It is imperative that all C. m. subsp. sepedonicus positive controls and samples are treated separately to avoid contamination.
Zelo pomembno je, da se pozitivne kontrole in vzorci C. m. subsp. sepedonicus obravnavajo ločeno, da se izognemo okužbi.
This applies to IF slides and to all tests.
To velja za stekelca IF in vse teste.
For detection of latent C. m. subsp. sepedonicus populations it is advised to test composite samples.
Za odkrivanje latentnih populacij C. m. subsp. sepedonicus, je priporočljivo testirati sestavljene vzorce.
The procedure can be conveniently applied for composite samples of up to 200 stem parts.
Postopek se lahko ustrezno uporabi za sestavljene vzorce z do 200 delov stebel.
(Where surveys are performed they should be based on a statistically representative sample of the plant population under investigation.)
(Kadar se opravljajo raziskave, morajo temeljiti na statistično reprezentativnem vzorcu rastlinske populacije, ki jo proučujemo.)
With a clean disinfected knife or pruning shears, remove a 1 to 2 cm segment from the base of each stem, just above the soil level.
S čistim razkuženim nožem ali vrtnarskimi škarjami odstranimo 1-2 centimetrski del iz spodnjega dela stebla, tik nad površino zemlje.
Disinfect stem segments briefly with ethanol 70 % and immediately blot dry on tissue paper.
Na kratko razkužimo dele stebel s 70 % etanolom in nemudoma popivnamo s pivnim papirjem.
Collect stem segments in a closed sterile container according to the following sampling procedures:
Dele stebel damo v zaprto sterilno posodo v skladu z naslednjimi postopkom vzorčenja:
Process the stem segments by one of the following procedures: either,
Dele stebel obdelamo po enem od naslednjih postopkov:
cover the segments with sufficient volume (approximately 40 ml) of extraction buffer (Appendix 3) and agitate on a rotary shaker (50 to 100 rpm) for four hours below 24 °C or for 16 to 24 hours refrigerated,
Prekrijemo delce z zadostno količino (približno 40 ml) ekstrakcijskega pufra (Dodatek 3) in pretresemo na rotacijskem stresalniku (50-100 vrt/min) 4 ure pri temperaturi pod 24 °C in 16 do 24 ur pri temperaturi hladilnika;
process immediately. By crushing the segments in a strong maceration bag (e.g. Stomacher or Bioreba) with an appropriate volume of extraction buffer (Appendix 3) using a rubber mallet or appropriate grinding apparatus (e.g. Homex).
nemudoma obdelamo z mletjem delov v močni maceracijski vrečki (npr. Stomacher ali Bioreba) z ustrezno količino ekstrakcijskega pufra (Dodatek 3) in gumijastim batom ali ustrezno aparaturo za mletje (npr. Homex).
If this is not possible, store the stem segments refrigerated for not longer than 72 hours or for not longer than 24 hours at room temperature.
Če to ni mogoče, shranimo dele stebel v hladilniku za največ 72 ur ali za največ 24 ur na sobni temperaturi.
Decant the supernatant after settling for 15 minutes.
Odlijemo supernatant, potem ko pustimo 15 minut, da se usedlina usede.
Further clarification of the extract or concentration of the bacterial fraction are not usually required but may be achieved by filtration and/or centrifugation as described in section 3.1.4 to 3.1.6.
Nadaljnje čiščenje ekstrakta ali koncentracije bakterijske frakcije navadno ni potrebno, lahko pa ga dosežemo s filtriranjem in/ali centrifugiranjem, kot je opisano v oddelku 3.1.4 do 3.1.6.
Divide the neat or concentrated sample extract into 2 equal parts.
Razdelimo čisti ali koncentriran ekstrakt vzorca na dva enaka dela.
Maintain one half at 4 to 10 °C during testing and store the other half with 10 to 25 % (v/v) sterile glycerol at –16 to –24 °C (weeks) or at –68 to –86 °C (months) in case further testing is required.
Eno polovico med testiranjem hranimo pri 4 do 10 °C, drugo pa shranimo z 10-25 % (v/v) sterilnim glicerolom pri –16 do –24 °C (tedni) ali pri –68 do –86 °C (meseci), če je potrebno nadaljnje testiranje.
The use of the IF test as the principal screening test is recommended because of its proven robustness to achieve the required thresholds.
Uporaba testa IF kot glavnega presejalnega testa je priporočena, ker je dokazano obstojen pri doseganju zahtevanih pragov.
When the IF test is used as the principal screening test and the IF reading is positive, the PCR or FISH test must be performed as a second screening test.
Kadar se uporabi test IF kot glavni presejalni test in je odčitek IF pozitiven, se kot drugi presejalni test opravita testa PCR ali FISH.
When the IF test is used as the second screening test and the IF reading is positive, further testing according to the flow scheme is required to complete the analysis.
Kadar se test IF uporabi kot drugi presejalni test in je odčitek IF pozitiven, je za dokončanje analize potrebno nadaljnje testiranje v skladu z diagramom poteka.
Always use a polyclonal antibody, when the IF test is used as the principal screening test.
Vedno uporabimo poliklonsko protitelo, kadar se test IF uporabi kot glavni presejalni test.
In case of a positive IF reading with a polyclonal antibody further screening of the sample with a monoclonal antibody may provide more specificity but can be less sensitive.
V primeru pozitivnega odčitka IF pri poliklonskem protitelesu nadaljnje preverjanje vzorca z monoklonskim protitelesom lahko poskrbi za večjo natančnost vendar je lahko manj občutljivo.
Use antibodies to a reference strain of C. m. subsp. sepedonicus.
Uporabimo protitelesa na referenčni sev C. m. subsp. sepedonicus.
It is recommended that the titre is determined for each new batch of antibodies.
Priporočljivo je, da se titer določi za vsako novo serijo protiteles.
The titre is defined as the highest dilution at which optimum reaction occurs when testing a suspension containing 105 to 106 cells per ml of the homologous strain of C. m. subsp. sepedonicus and using an appropriate fluorescein isothiocyanate (FITC) conjugate according to the manufacturer's recommendations.
Titer je opredeljen kot največja razredčina, pri kateri se doseže optimalna reakcija, kadar testiramo suspenzijo z 105 do 106 celic na ml homolognega seva C. m. subsp. sepedonicus in uporabljamo ustrezno razredčino fluorescein izotiocinatovega (FITC) konjugata v skladu z navodili proizvajalca.
The crude polyclonal or monoclonal antibodies should have an IF titre of at least 1:2000.
Surova poliklonska ali monoklonska protitelesa morajo imeti titer IF vsaj 1:2000.
During testing, the antibodies should be used at a working dilution(s) (WD) close to or at the titre.
Med testiranjem je treba protitelesa uporabljati na delovni(-ih) razredčini(-ah), ki je (so) blizu ali enaka(-e) titru.
Use validated antibodies (see web site http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Uporabimo potrjena protitelesa (glej spletno stran http://forum.europa.eu.int/Public/irc/sanco/Home/main).
The test should be performed on freshly-prepared sample extracts.
Test je treba opravi na sveže pripravljenih ekstraktih vzorcev.
If necessary, it can be successfully performed on extracts stored at –68 to –86 °C under glycerol.
Če je potrebno, se lahko uspešno opravi na ekstraktih, hranjenih pri –68 do –86 °C pod glicerolom.
Glycerol can be removed from the sample by addition of 1 ml pellet buffer (Appendix 4), re-centrifugation for 15 minutes at 7000 g and resuspension in an equal volume of pellet buffer.
Glicerol se lahko odstrani iz vzorca z dodajanjem 1 ml peletnega pufra (Dodatek 4), ponovnim 15-minutnim centrifugiranjem na 7000 g in resuspenzijo v enaki prostornini peletnega pufra.
This is often not necessary, especially if slides samples are fixed to the slides by flaming (see 2.2).
To pogosto ni potrebno, zlasti če so vzorci na objektna stekelca fiksirani nad plamenom (glej 2.2).
Prepare separate positive control slides of the homologous strain or any other reference strain of C. m. subsp. sepedonicus, suspended in potato extract, as specified in Appendix 2, and optionally in buffer.
Pripravimo ločena objektna stekelca pozitivne kontrole homolognega seva ali katerega koli referenčnega seva C. m. subsp. sepedonicus, suspendiranega v ekstraktu krompirja, kot je določeno v Dodatku 2, ali po izbiri v pufru.
Naturally infected tissue (maintained by lyophilization or freezing at –16 to –24 °C) should be used where possible as a similar control on the same slide.
Če je mogoče, uporabimo naravno okuženo tkivo (ohranjeno z liofilizacijo ali zamrznitvijo pri temperaturi –16 do –24 °C) kot podobno kontrolo na istem objektnem stekelcu.
As negative controls, use aliquots of sample extract which previously tested negative.
Za negativne kontrole uporabimo alikvote ekstrakta vzorca, ki je bil predhodno testiran z negativnim rezultatom.
Use multiwell microscope slides with preferably 10 windows of at least 6 mm diameter.
Uporabimo mikroskopska objektna stekelca z več vdolbinicami z po možnosti 10 okenci premera vsaj 6 mm.
Test control material in an identical manner as the sample(s).
Kontrolni material testiramo na enak način kot vzorec ali vzorce.
Prepare the test slides by one of the following procedures:
Testna objektna stekelca pripravimo po enem od naslednjih postopkov:
For pellets with relatively little starch sediment:
Za pelete z relativno majhno škrobno usedlino:
Pipette a measured standard volume (15 µl is appropriate for 6 mm window diameter-scale up volume for larger windows) of a 1/100 dilution of the resuspended potato pellet onto the first window.
S pipeto kanemo odmerjeno standardno količino (15 µl je primerno za okenca s premerom 6 mm - povečamo količino za večja okenca) razredčine 1/100 resuspendirane krompirjeve pelete na prvo okence.
Subsequently pipette a similar volume of undiluted pellet (1/1) onto the remaining windows on the row.
Nato s pipeto kanemo podobno količino nerazredčene pelete (1/1) na preostala okenca v vrsti.
The second row can be used as duplicate or for a second sample as presented in Figure 1.
Druga vrsta se lahko uporabi kot ponovitev ali za drugi vzorec, kot je prikazano na sliki 1.
For other pellets:
Za druge pelete:
Prepare decimal dilutions (1/10 and 1/100) of the resuspended pellet in pellet buffer.
Pripravimo desetkratne razredčine (1/10 in 1/100) resuspendirane pelete v peletnem pufru.
Pipette a measured standard volume (15 µl is appropriate for 6 mm window diameter-scale up volume for larger windows) of the resuspended pellet and each dilution on a row of windows.
S pipeto kanemo odmerjeno standardno količino (15 µl je primerno za okenca s premerom 6 mm - povečamo količino za večja okenca) resuspendirane pelete in vsake razredčine na vrsto okenc.
The second row can be used as duplicate or for a second sample as presented in Figure 2.
Druga vrsta se lahko uporabi kot ponovitev ali za drugi vzorec, kot je prikazano na sliki 2.
Dry the droplets at ambient temperatures or by warming at temperature of 40 to 45 °C.
Kapljice posušimo na sobni temperaturi ali s segrevanjem pri temperaturah od 40 do 45 °C.
Fix the bacterial cells to the slide either by heating (15 minutes at 60 °C), flaming, with 95 % ethanol or according to specific instructions from the suppliers of the antibodies.
Fiksiramo bakterijske celice na objektno stekelce bodisi s segrevanjem (15 min pri 60 °C), nad plamenom, s 95-odstotnim etanolom ali v skladu s posebnimi navodili dobavitelja protiteles.
If necessary, fixed slides may then be stored frozen in a desiccated box for as little time as necessary (up to a maximum of 3 months) prior to further testing.
Po potrebi se fiksirana objektna stekelca lahko shranijo zmrznjena v sušilni posodi, za kolikor je mogoče kratek čas (do največ 3 mesece) pred nadaljnjim testiranjem.
IF procedure:
Postopek IF:
According to test slide preparation in 4.1(i):
V skladu s pripravo objektnih stekelc v 4.1(i):
Prepare a set of twofold dilutions of the antibody in IF buffer.
Pripravimo niz dvakratnih razredčin protitelesa v pufru IF.
The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).
Prva vdolbinica mora imeti 1/2 titra (T/2), druge pa 1/4 titra (T/4), 1/2 titra (T/2), titer (T) in dvakratni titer (2T).
According to test slide preparation in 4.1(ii):
V skladu s pripravo objektnih stekelc v 4.1(ii):
Prepare the working dilution (WD) of the antibody in IF buffer.
Pripravimo delovno razredčino (WD) protitelesa v pufru IF.