natgillin/translations-raw huggingface dataset:
Done at Brussels, 12 June 2006.
V Bruslju, 12. junija 2006
Commission Directive 2006/56/EC
Direktiva Komisije 2006/56/ES
amending the Annexes to Council Directive 93/85/EEC on the control of potato ring rot
o spremembi prilog k Direktivi Sveta 93/85/EGS o obvladovanju krompirjeve obročkaste gnilobe
Having regard to Council Directive 93/85/EEC of 4 October 1993 [1] on the control of potato ring rot, and in particular Article 12 thereof,
ob upoštevanju Direktive Sveta 93/85/EGS z dne 4. oktobra 1993 [1] o obvladovanju krompirjeve obročkaste gnilobe in zlasti člena 12 Direktive,
One of the important organisms harmful to potatoes is Clavibacter michiganensis (Smith) Davis et al. ssp. sepedonicus (Spieckermann et Kotthoff) Davis et al., the pathogenic agent of the potato ring rot disease (hereinafter referred to as ‘the organism’).
Eden izmed pomembnih škodljivih organizmov za krompir bakterija Clavibacter michiganensis (Smith) Davis et al. ssp. sepedonicus (Spieckermann et Kotthoff) Davis et al., povzročiteljica krompirjeve obročkaste gnilobe (v nadaljnjem besedilu „organizem“).
The organism still occurs in some parts of the Community.
Organizem se še vedno pojavlja ponekod v Skupnosti.
Council Directive 93/85/EEC has laid down detailed measures to be taken within the Member States against the organism in order to locate it and determine its distribution; prevent its occurrence and spread; and if found, to prevent its spread and to control it with the aim of eradication.
Direktiva Sveta 93/85/EGS je določila podrobne ukrepe, ki jih morajo države članice sprejeti proti temu organizmu, da se določi kraj in obseg njegove razširjenosti, preprečijo njegovo pojavljanje in širjenje; in če se odkrije, prepreči njegovo širjenje in da se obvladuje z namenom izkoreninjenja.
Since then, there have been significant developments in the understanding of the biology, the detection and identification procedures of the organism; moreover practical experiences gained in controlling the organism calls for review of several technical provisions related to control measures.
Od takrat je prišlo do bistvenega napredka pri razumevanju biologije ter postopkih odkrivanja in identifikacije organizma; poleg tega praktične izkušnje, pridobljene pri obvladovanju organizma, zahtevajo ponovni pregled nekaterih tehničnih določb, ki se nanašajo na ukrepe za obvladovanje.
As a result of such developments, it appears necessary to review and update the measures included in the Annexes to Directive 93/85/EEC.
Kot rezultat tega razvoja, je treba ponovno preveriti in posodobiti ukrepe, vključene v priloge k Direktivi 93/85/EGS.
As regards the detection and identification procedures, recently developed procedures like fluorescent in situ hybridization (FISH) and polymerase chain reaction (PCR) are incorporated as well as improvements of various technical elements of the current detection and identification procedure.
Glede postopkov odkrivanja in identifikacije so vključeni tudi nedavno razviti postopki, kot sta fluorescentna in-situ hibridizacija (FISH) in verižna reakcija s polimerazo (PCR), prav tako pa tudi izboljšave različnih tehničnih elementov trenutnih postopkov odkrivanja in identifikacije.
As regards the technical elements of the control measures, improved provisions are made for: the way of conservation of tested samples in order to ensure trace back of the organism, the elements needed to determine the extent of the probable contamination, the details of the notification of any confirmed presence of the organism and of the relevant contaminated zone, measures to implement in places of production designated as contaminated and within the demarcated zones.
Izboljšane določbe glede tehničnih elementov ukrepov za obvladovanje so uvedene v zvezi z: načinom konzerviranja testiranih vzorcev za zagotavljanje izsleditve organizma, elementov za ugotavljanje obsega verjetne okužbe, podrobnostmi v zvezi z obveščanjem o potrjeni navzočnosti organizma in ustreznem okuženem območju, izvedbenimi ukrepi na mestih pridelave, ki so bila določena za okužena in v razmejenih območjih.
The Annexes to Directive 93/85/EEC are hereby replaced by the corresponding texts in Annex to this Directive.
Priloge k Direktivi 93/85/EGS se nadomestijo z ustreznim besedilom Priloge k tej direktivi.
Member States shall adopt and publish, by 31 March 2007 at the latest, the laws, regulations and administrative provisions necessary to comply with this Directive.
Države članice sprejmejo in objavijo zakone in druge predpise, potrebne za uskladitev s to direktivo, najpozneje do 31. marca 2007.
They shall forthwith communicate to the Commission the text of those provisions and a correlation table between those provisions and the Directive.
Komisiji takoj sporočijo besedilo navedenih določb in primerjalno tabelo med navedenimi določbami in Direktivo.
Member States shall immediately communicate to the Commission the text of the main provisions of national law which they adopt in the field covered by this Directive.
Države članice Komisiji takoj predložijo besedila temeljnih predpisov nacionalne zakonodaje, sprejetih na področju, ki ga ureja ta direktiva.
OJ L 259, 18.10.1993, p. 2.
UL L 259, 18.10.1993, str. 2.
TEST SCHEME FOR DIAGNOSIS, DETECTION AND IDENTIFICATION OF THE RING ROT BACTERIUM, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp.
TESTNA SHEMA ZA DIAGNOSTICIRANJE, ODKRIVANJE IN IDENTIFIKACIJO BAKTERIJE KROMPIRJEVE OBROČKASTE GNILOBE, CLAVIBACTER MICHIGANENSIS (Smith) Davis et al. ssp.
SCOPE OF THE TEST SCHEME
PODROČJE UPORABE TESTNE SHEME
The presented scheme describes the various procedures involved in:
Ta shema opisuje različne postopke za:
Diagnosis of ring rot in potato tubers and plants;
Diagnosticiranje krompirjeve obročkaste gnilobe v gomoljih in rastlinah krompirja;
Detection of Clavibacter michiganensis ssp. sepedonicus in samples of potato tubers and plants;
Odkrivanje bakterije Clavibacter michiganensis ssp. sepedonicus v vzorcih gomoljev in rastlin krompirjeva;
Identification of Clavibacter michiganensis ssp. sepedonicus (C. m. subsp. sepedonicus).
Identifikacija Clavibacter michiganensis ssp. sepedonicus (C. m. subsp. sepedonicus).
Optimized protocols for the various methods, validated reagents and details for the preparation of test and control materials are provided in the Appendices.
V dodatkih so določeni optimizirani protokoli različnih metod, potrjenih reagentov in podrobnosti za pripravo testnih in kontrolnih materialov.
A list of the laboratories that were included in optimization and validation of protocols is provided in Appendix 1.
V Dodatku 1 je naveden seznam laboratorijev, ki so vključeni v optimizacijo in potrjevanje protokolov.
Since the protocols involve detection of a quarantine organism and will include the use of viable cultures of C. m. subsp. sepedonicus as control materials, it will be necessary to perform the procedures under suitable quarantined conditions with adequate waste disposal facilities and under the conditions of appropriate licences as issued by the official plant quarantine authorities.
Ker protokoli vključujejo odkrivanje karantenskega organizma in bodo vključevali uporabo kultur C. m. subsp. sepedonicus kot kontrolnih materialov, ki so sposobne preživeti, bo treba postopke opravljati v primernih karantenskih pogojih z ustreznimi napravami za odstranjevanje odpadkov in na podlagi ustreznih dovoljenj, ki jih izdajo uradni organi za rastlinsko karanteno.
Testing parameters must assure consistent and reproducible detection of levels of C. m. subsp. sepedonicus at the set thresholds of the selected methods.
Parametri testiranja morajo zagotoviti dosledno in ponovljivo odkrivanje stopenj C. m. subsp. sepedonicus na določenih pragovih izbranih metod.
Precise preparation of positive controls is imperative.
Nujna je natančna priprava pozitivnih kontrol.
Testing according to the required thresholds also implies correct settings, maintenance and calibration of equipment, careful handling and preservation of reagents and all measures to prevent contamination between samples, e.g. separation of positive controls from test samples.
Za testiranje po zahtevanih pragih so potrebne pravilne nastavitve, vzdrževanje in umerjanje opreme, previdnost pri ravnanju z reagenti in njihovo ohranjanje ter vsi ukrepi za preprečevanje okužbe med vzorci, npr. ločitev pozitivnih kontrol od testnih vzorcev.
Quality control standards must be applied to avoid administrative and other errors, especially concerning labelling and documentation.
Uporabljati se morajo standardi obvladovanja kakovosti, da bi se lahko izognili upravnim in drugim napakam, zlasti pri označevanju in dokumentaciji.
A suspected occurrence, as referred to in article 4(2) in directive 93/85/EEC implies a positive result in diagnostic or screening tests performed on a sample as specified in flow charts.
Domnevi pojav, kot je določeno v členu 4(2) Direktive 93/85/EGS, pomeni pozitivni rezultat pri diagnostičnih ali presejalnih testih, ki se opravijo na vzorcu, kot je določeno v puščičnih diagramih.
If the first screening test (IF or PCR/FISH) is positive, then contamination with Cms is suspected and a second screening test must be done.
Če je prvi presejalni test (IF ali PCR/FISH) pozitiven, potem se sumi na okužbo z bakterijo Cms in opraviti se mora drugi presejalni test.
If the second screening test is positive, then the suspicion is confirmed (suspected occurrence) and the testing according to the scheme must be continued.
Če je drugi presejalni test pozitiven, potem je sum potrjen (domnevni pojav) in testiranje v skladu s shemo se mora nadaljevati.
If the second screening test is negative, then the sample is considered not contaminated with Cms.
Če je drugi presejalni test negativen, potem se šteje, da vzorec ni okužen z bakterijo Cms.
Therefore a positive IF test as referred to in article 4(2) is defined by a positive IF reading confirmed by a second screening test (PCR/FISH).
Torej je pozitivni IF test, kot je določen v členu 4(2) Direktive 93/85/EGS, opredeljen kot pozitivni rezultat IF, ki je potrjen z drugim presejalnim testom (PCR/FISH).
Confirmed presence as referred to in article 5(1) in directive 93/85/EEC implies the isolation and identification of a pure culture of C. m. subsp. sepedonicus with confirmation of pathogenicity.
Potrjena navzočnost, kot je določena v členu 5(1) Direktive 93/85/EGS pomeni izolacijo in identifikacijo čiste kulture C. m. subsp. sepedonicus s potrditvijo patogenosti.
FLOW CHART DIAGRAM PRESENTATION
PREDSTAVITEV S PUŠČIČNIMI DIAGRAMI
Detection scheme for the diagnosis of Ring Rot in potato tubers and potato plants with symptoms of ring rot
Shema odkrivanja za diagnosticiranje obročkaste gnilobe v gomoljih in rastlinah krompirja s simptomi obročkaste gnilobe
The testing procedure is intended for potato tubers and plants with symptoms typical or suspect of ring rot.
Postopek testiranja je namenjen gomoljem in rastlinam krompirja z značilnimi simptomi obročkaste gnilobe ali simptomi, ki vzbujajo sum na obročkasto gnilobo.
It involves a rapid screening test, isolation of the pathogen from infected vascular tissue on diagnostic media and, in case of a positive result, identification of the culture as C. m. subsp. sepedonicus. potato tuber(s) or potato plant(s) with symptoms
Vključuje hitri presejalni test, izolacijo patogena iz okuženega vaskularnega tkiva na diagnostičnem gojišču in v primeru pozitivnega rezultata identifikacijo kulture kot bakterije C. m. subsp. sepedonicus.
suspect of ring rot (1)
Gomolj(-i) ali rastlina(-e) krompirja s simptomi, po katerih se sumi na krompirjevo gnilobo (1)
RAPID DIAGNOSTIC TESTS (2)
HITRI DIAGNOSTIČNI TESTI (2)
CORE ISOLATION TEST (3)
IZOLACIJSKI TEST JEDER (3)
colonies with typical morphology (4)
Kolonije z značilno morfologijo (4)
subsp. sepedonicus
z C.m. subsp. sepedonicus
purify by subculturing
Prečistimo s precepljanjem
IDENTIFICATION TESTS (6)
IDENTIFIKACIJSKI TESTI (6)
PATHOGENICITY TEST (7)
TESTI PATOGENOSTI (7)
both tests confirm pure culture as C.m. subsp. sepedonicus
Oba testa potrjujeta čisto kulturo kot C.m. subsp. sepedonicus
sample not infected by
Vzorec ni okužen z
sample infected by
Vzorec je okužen z
Description of symptoms is provided in section 2.
Opis simptomov je naveden v oddelku 2.
Appropriate tests are:
Primerni testi so:
IF-test (section 4),
test IF (oddelek 4),
PCR test (section 6),
test PCR (oddelek 6),
FISH test (section 5).
test FISH (oddelek 5).
Although isolation of the pathogen from plant material with typical symptoms by dilution plating is straightforward, culturing may fail from advanced stages of infection.
Čeprav je izolacija patogena iz rastlinskega materiala z značilnimi simptomi z razmazom razredčine preprosta, lahko kultiviranje spodleti pri naprednih fazah okužbe.
Saprophytic bacteria which grow on diseased tissue may outgrow or inhibit the pathogen on the isolation medium.
Saprofitske bakterije, ki rastejo na obolelem tkivu, lahko prerastejo ali inhibirajo patogene na izolacijskem gojišču.
Therefore it is recommended to use both non selective and selective media, preferably MTNA (section 8) or Bioassay test (section 7).
Zato je priporočljivo uporabiti tako neselektivno kot selektivno gojišče, po možnosti MTNA (oddelek 8) ali biološki test (oddelek 7).
Description of typical colony morphology is provided in section 8.
Opis značilne morfologije kolonije je naveden v oddelku 8.
If the isolation test is negative, but disease symptoms are typical, then isolation must be repeated.
Če je izolacijski test negativen, simptomi bolezni pa so značilni, je treba postopek izolacije ponoviti.
Reliable identification of a pure culture of C. m. subsp. sepedonicus is achieved by using the tests listed in section 9.
Zanesljiva identifikacija čiste kulture C.m. subsp. sepedonicus se doseže z uporabo testov, naštetih v oddelku 9.
The pathogenicity test is described in section 10.
Test patogenosti je opisan v oddelku 10.
Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato tubers
Shema za odkrivanje in identifikacijo bakterije Clavibacter michiganensis ssp. sepedonicus v vzorcih asimptomatičnih gomoljev krompirja
The testing procedure is intended for detection of latent infections in potato tubers.
Postopek testiranja je namenjen odkrivanju latentnih okužb v gomoljih krompirja.
A positive result from at least two screening tests, based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as C. m. subsp. sepedonicus.
Pozitiven rezultat vsaj dveh presejalnih testov, ki temeljita na različnih bioloških načelih, mora biti dopolnjen z izolacijo patogena, ki ji v primeru izolacije tipičnih kolonij sledi potrditev čiste kulture kot C. m. subsp. sepedonicus.
A positive result from only one of the screening tests is not sufficient to consider the sample suspect.
Pozitiven rezultat samo enega presejalnega testa ni dovolj, da bi vzorec obravnavali kot sumljiv.
Screening tests and isolation tests must permit a detection threshold of 103 to 104 cells/ml resuspended pellet, included as positive controls in each series of tests.
Presejalni testi in izolacijski testi morajo dopuščati prag odkrivanja 103 do 104 celic/ml resuspendirane pelete, vključene kot pozitivne kontrole v vsaki seriji testov.
sample of potato tubers (1)
Vzorec delov gomolja krompirja (1)
extraction and concentration of the pathogen (2)
Ekstrakcija in koncentracija patogena (2)
CORE SCREENING TESTS (3)
PRESEJALNI TESTI JEDER (3)
first screening test (3)
Prvi presejalni test (3)
subsp. sepedonicus not detected
subsp. sepedonicus ni odkrita
positive
pozitiven
second screening test (3)
Drugi presejalni test (3)
selective isolation (7) and bioassay (8)
Selektivna izolacija (7) in biološki test (8)
colonies with typical morphology (9)
Kolonija z značilno morfologijo (9)
purify by subculture
Prečistimo s precepljanjem
IDENTIFICATION TESTS (11)
IDENTIFIKACIJSKI TESTI (11)
PATHOGENICITY TEST (12)
TESTI PATOGENOSTI (12)
both tests confirm pure culture as
Oba testa potrjujeta čisto kulturo kot
The standard sample size is 200 tubers although the procedure can be used with smaller samples if 200 tubers are not available.
Standardna velikost je 200 gomoljev. Vendar je ta postopek mogoče ustrezno uporabiti tudi za vzorce z manj kot 200 gomolji, če jih toliko ni na voljo.
Pathogen extraction and concentration methods are described in section 3.1.
Metode ekstrakcija in koncentracije patogena so opisane v oddelku 3.1.
If at least two tests based on different biological principles are positive, isolation and confirmation have to be done.
Če sta vsaj dva testa, ki temeljita na različnih bioloških načelih, pozitivna, je treba opraviti izolacijo in potrditev.
Perform at least one screening test.
Opravimo vsaj en presejalni test.
When this test is negative the sample is considered to be negative In case this test is positive a second or more screening tests based on different biological principles are required to verify the first positive result.
Če je ta test negativen, se vzorec šteje za negativnega. Če je ta test pozitiven, je treba opraviti drugi ali več presejalnih testov, ki temeljijo na različnih bioloških načelih, da se preveri prvi pozitivni rezultat.
If the second or other tests are negative the sample is considered negative.
Če so drugi test ali ostali testi negativni, se vzorec šteje za negativnega.
Further tests are not necessary.
Nadaljnji testi niso potrebni.
Immunofluorescence (IF) test.
Vedno uporabimo poliklonsko protitelo za presejanje IF, dodatna monoklonska protitelesa lahko zagotovijo večjo specifičnost
Always use a polyclonal antibody for IF screening, additional monoclonal antibodies may provide more specifity (see section 4).
(glej oddelek 4).
Use appropriately validated PCR reagents and protocols (see section 6).
Uporabimo ustrezno potrjene reagente in protokole PCR (glej oddelek 6).
Use validated reagents and protocols (see section 5).
Uporabimo potrjene reagente in protokole (glej oddelek 5).
Selective isolation.
Selektivna izolacija.
With the MTNA medium or NCP-88 medium and a 1/100 dilution of the resuspended pellet, this is in many cases a suitable method for direct isolation of C. m. subsp. sepedonicus.
Z gojiščem MTNA ali NCP-88 in 1/100 razredčino resuspendirane pelete je to v mnogih primerih ustrezna metoda za neposredno izolacijo C. m. subsp. sepedonicus.
Typical colonies can be obtained 3 to10 days after planting.
Značilne kolonije se lahko pridobijo 3–10 dni po pripravi vzorca.
The pathogen then can be purified and identified.
Patogen se potem lahko prečisti in identificira.
For full exploitation of its potential, the test requires careful preparation of heel end cores to avoid secondary bacteria associated with the potato tuber which are competitors with C. m. subsp. sepedonicus on the medium and may overgrow the pathogen.
Za popolnoma izkoriščeno zmogljivost testa je potrebna natančna priprava jedra stolona, da se izognemo sekundarnim bakterijam, povezanim z gomoljem krompirja, ki na gojišču tekmujejo z bakterijo C. m. subsp. sepedonicus in prerastejo patogene.
If the plate test fails isolation must be done from plants used for the bio assay (see section 8).
Če test z razmazom na gojišče spodleti, se mora izolacija opraviti iz rastlin, ki se uporabijo za biološki test (glej oddelek 8).
The bioassay test is used for isolation of C. m. subsp. sepedonicus from potato extract pellets by selective enrichment in eggplants (Solanum melongena).
Biološki test se uporablja za izolacijo bakterije C. m. subsp. sepedonicus iz pelet krompirjevega ekstrakta s selektivno obogatitvijo v jajčevcih (Solanum melongena).
The test requires optimal incubation conditions as specified in this method.
Test zahteva optimalne pogoje inkubacije, kakor jih opredeljuje metoda.
Bacteria inhibitory to C. m. subsp. sepedonicus on the MTNA or NCP-88 medium will most likely not interfere in this test (see section 7).
Bakterije, ki inhibirajo bakterijo C. m. subsp. sepedonicus na gojišču MTNA ali NCP-88, testa najverjetneje ne bodo motile (glej oddelek 7).
Typical colony morphology is described in section 8.
Značilna morfologija kolonije je opisana v oddelku 8.
Culturing or bioassays can fail due to competition or inhibition by saprophytic bacteria.
Kultiviranje ali biološki testi lahko spodletijo zaradi tekmovanja ali inhibicije sprofitskih bakterij.
If positive results are obtained in screening tests, but the isolation tests are negative, then repeat the isolation tests from the same pellet or by taking additional vascular tissue near the heel end from cut tubers of the same sample and, if necessary, test additional samples.
Če s presejalnimi testi dobimo pozitivne rezultate, izolacijski testi pa so negativni, ponovimo izolacijski test iz iste pelete ali z odvzemom dodatnega vaskularnega tkiva blizu stolona razrezanih gomoljev istega vzorca in po potrebi testiramo dodatne vzorce.
Reliable identification of pure presumptive C. m. subsp. sepedonicus cultures is achieved using the tests described in section 9.
Zanesljiva identifikacija čistih kultur domnevne C. m. subsp. sepedonicus se doseže z uporabo testov, opisanih v oddelku 9.
Scheme for detection and identification of Clavibacter michiganensis ssp. sepedonicus in samples of asymptomatic potato plants
Shema za odkrivanje in identifikacijo bakterije Clavibacter michiganensis ssp. sepedonicus v vzorcih asimptomatičnih rastlin krompirja
sample of stem segments (1)
Vzorec delov stebla krompirja (1)
selective isolation (4)/IF test (5)/PCR test (6)/FISH test (7)
selektivna izolacija (3)/test IF (5)/test PCR (6)/test FISH (7)
optional auxiliary test: bioassay (8)
izbirni dopolnilni test: biološki preskus (8)
colonies with typical morphology (4) after isolation
Kolonije z značilno morfologijo (4) po izolaciji
all tests done negative
Vsi opravljeni testi negativni
IDENTIFICATION TESTS (10)
IDENTIFIKACIJSKI TESTI (10)
PATHOGENICITY TEST (11)
TEST PATOGENOSTI (11)
See section 3.2 for recommended sample sizes.
Za priporočene velikosti vzorcev glej oddelek 3.2.
Pathogen extraction and concentration methods are described in section 3.2.
Metode ekstrakcije in koncentracije patogena so opisane v oddelku 3.2.
When this test is negative the sample is considered to be negative.
Če je ta test negativen, se vzorec šteje za negativnega.
In case this test is positive a second or more screening tests based on different biological principles are required to verify the first positive result.
Če je ta test pozitiven, je treba opraviti drugi ali več presejalnih testov, ki temeljijo na različnih bioloških načelih, da se preveri prvi pozitivni rezultat.
The selective isolation test and typical colony morphology are described in section 8.
Test selektivne izolacije in značilna morfologija kolonije je opisana v oddelku 8.
The IF test is described in section 4.
Test IF je opisan v oddelku 4.
PCR tests are described in section 6.
Testi PCR so opisani v oddelku 6.
The FISH test is described in section 5.
Test FISH je opisan v oddelku 5.
The bioassay is described in section 7.
Biološki test je opisan v oddelku 7.
If positive results are obtained in screening tests, but the isolation tests are negative, then repeat the isolation tests and, if necessary, test additional samples.
Če s presejalnimi testi dobimo pozitivne rezultate, izolacijski testi pa so negativni, ponovimo izolacijski test in po potrebi testiramo dodatne vzorce.
VISUAL EXAMINATION FOR RING ROT SYMPTOMS
VIZUALNI PREGLED ZA ODKRIVANJE SIMPTOMOV OBROČKASTE GNILOBE
Potato plants
Rastline Krompirja
Under European climatic conditions symptoms are rarely found in the field and often only at the end of the season.
V evropskih vremenskih razmerah redko najdemo simptome na polju in pogosto samo ob koncu sezone.
Moreover the symptoms are frequently masked or confused by/with other diseases, senescence or mechanical damages.
Poleg tega so simptomi pogosto prikriti ali se zamenjujejo z drugimi boleznimi, staranjem ali mehanskimi poškodbami.
Therefore it may be easy to miss symptoms in field inspections.
Zato lahko zlahka spregledamo simptome na terenskih uradnih pregledih.