natgillin/translations-raw huggingface dataset:
Serological agglutination tests on bacterial ooze or extracts from symptomatic tissue are described in Section VI.A.3.
Testi serološke aglutinacije na bakterijskem izcedku ali izvlečki iz simptomatskega tkiva so opisani v oddelku VI.A.3.
IF test on bacterial ooze suspended in water or symptomatic tissue extracts is described in Section VI.A.5.
IF test na bakterijskem izcedku, suspendiranem v vodi, ali na izvlečku simptomatskega tkiva je opisan v oddelku VI.A.5.
FISH test on bacterial ooze suspended in water or symptomatic tissue extracts is described in Section VI.A.7.
Test FISH na bakterijskem izcedku, suspendiranem v vodi, ali na izvlečku simptomatskega tkiva je opisan v oddelku VI.A.7.
ELISA test on bacterial ooze suspended in water or symptomatic tissue extracts is described in Section VI.A.8.
Test ELISA na bakterijskem izcedku, suspendiranem v vodi, ali na izvlečku simptomatskega tkiva je opisan v oddelku VI.A.8.
PCR test on bacterial ooze suspended in water or symptomatic tissue extracts is described in Section VI.A.6.
Test PCR na bakterijskem izcedku, suspendiranem v vodi, ali na izvlečku simptomatskega tkiva je opisan v oddelku VI.A.6.
The pathogen is usually easily isolated from symptomatic plant material by dilution plating (Section II.3).
Patogen je običajno preprosto izolirati od simptomatskega rastlinskega materiala z redčitvenim razmazom (oddelek II.3.).
Typical colony morphology is described in Section II.3.d.
Značilna morfologija kolonije je opisana v oddelku II.3.d.
Culturing may fail from advanced stages of infection due to competition or overgrowth by saprophytic bacteria.
Gojenje na naprednih stopnjah infekcije lahko spodleti zaradi tekmovanja s saprofitskimi bakterijami, ki lahko prerastejo patogen.
If disease symptoms are typical, but the isolation test is negative, then the isolation must be repeated, preferably using a selective plate test.
Če so simptomi bolezni značilni, vendar je izolacijski test negativen, je treba izolacijo ponoviti, po možnosti s testom selektivnega razmaza.
Reliable identification of pure cultures of presumptive R. solanacearum isolates is achieved using the tests described in Section VI.B.
Zanesljivo identifikacijo čistih kultur domnevnega izolata bakterije R. solanacearum dosežemo s testi, opisanimi v oddelku VI.B.
Sub-specific characterisation is optional but recommended for each new case.
Dodatna možnost je podspecifična opredelitev, vendar je za vsak nov primer priporočljiva.
The pathogenicity test is described in Section VI.C.
Test patogenosti je opisan v oddelku VI.C.
Scheme for detection and identification of Ralstonia solanacearum in samples of asymptomatic potato tubers
Shema za detekcijo in identifikacijo bakterije R. solanacearum v vzorcih asimptomatskih gomoljev krompirja
Principle:
Načelo:
The testing procedure is intended for detection of latent infections in potato tubers.
Postopek testiranja je namenjen detekciji latentnih okužb gomoljev krompirja.
A positive result from at least two screening tests (3), based on different biological principles, must be complemented by the isolation of the pathogen; followed by, in case of isolation of typical colonies, confirmation of a pure culture as R. solanacearum A positive result from only one of the screening tests is not sufficient to consider the sample suspect.
Pozitivni rezultat vsaj dveh presejalnih testov3, ki sta osnovana na različnih bioloških načelih, mora biti dopolnjen z izolacijo patogena, ki mu v primeru izolacije značilnih kolonij sledi potrditev čiste kulture kot R. solanacearum. Pozitiven rezultat samo enega od presejalnih testov ne zadošča za sum okuženosti vzorca.
Screening tests and isolation tests must permit detection of 103 to 104 cells/ml of resuspended pellet, included as positive controls in each series of tests.
Presejalni testi in izolacijski testi morajo dovoljevati detekcijo 103 do 104 celic/ml resuspendirane pelete, vključene kot pozitivne kontrole v vsaki seriji testov.
Sample of potato tubers (1)
Vzorec krompirjevih gomoljev (1)
Extraction and concentration of the pathogen (2)
Ekstrakcija in koncentracija patogena (2)
CORE SCREENING TESTS (3)
JEDRNI PRESEJALNI TESTI (3)
IF test (4) / selective isolation (5) / PCR test (6) / FISH test (7)
IF test (4) / selektivna izolacija (5) / PCR test (6) / FISH test (7)
Optional auxiliary test : ELISA (8)
Dodatni pomožni test: ELISA (8)
First screening test (3)
Prvi presejalni test (3)
sample not infected by R. solanacearum
Vzorec ni okužen z R. solanacearum
Negative
negativen
Positive
pozitiven
Second screening test (3)
Drugi presejalni test (3)
Selective isolation (5) and/or bioassay (9)
Selektivna izolacija (5) in/ali biološki test (9)
Colonies with typical morphology (10)
Kolonije z značilno morfologijo (10)
Purify by subculture
Prečistiti s subkulturo
IDENTIFICATION TESTS (12)
IDENTIFIKACIJSKI TESTI (12)
PATHOGENICITY TEST (13)
TEST PATOGENOSTI (13)
The standard sample size is 200 tubers, although the procedure can be used with smaller samples if 200 tubers are not available.
Velikost standardnega vzorca je 200 gomoljev, čeprav postopek lahko uporabimo z manjšimi vzorci, če 200 gomoljev ni na voljo.
Pathogen extraction and concentration methods are described in Section III.1.1.
Metode ekstrakcije in koncentracije patogena so opisane v oddelku III.1.1.
If at least two tests based on different biological principles are positive, isolation and confirmation have to be done Perform at least one screening test.
Če sta pozitivna vsaj dva testa, ki temeljita na različnih bioloških načelih, je treba opraviti izolacijo in potrditev. Opravite vsaj en presejalni test.
When this test is negative the sample is considered to be negative In case this test is positive a second or more screening tests based on different biological principles are required to verify the first positive result.
Če je ta test negativen, velja, da je vzorec negativen. Če je ta test pozitiven, je treba opraviti drugi presejalni test oziroma več testov, ki temeljijo na drugačnih bioloških načelih, da se preveri prvi pozitivni rezultat.
If the second or other tests are negative the sample is considered negative.
Če so drugi test oziroma ostali testi negativni, velja, da je vzorec negativen.
The selective isolation test is described in Section VI.A.4.
Test selektivne izolacije je opisan v oddelku VI.A.4.
PCR tests are described in Section VI.A.6.
Testi PCR so opisani v oddelku VI.A.6.
The FISH test is described in Section VI.A.7.
Test FISH je opisan v oddelku VI.A.7.
ELISA tests are described in Section VI.A.8.
Testi ELISA so opisani v oddelku VI.A.8.
The bioassay is described in Section VI.A.9.
Biološki test je opisan v oddelku VI.A.9.
Culturing or bioassays can fail due to competition or inhibition by saprophytic bacteria.
Gojenje ali biološki test lahko spodleti zaradi tekmovanja ali inhibicijskega delovanja saprofitskih bakterij.
If clear positive results are obtained in screening tests, but the isolation tests are negative, then repeat the isolation tests from the same pellet or by taking additional vascular tissue near the heel end from cut tubers of the same sample and, if necessary, test additional samples.
Če presejalni testi vrnejo jasne pozitivne rezultate, vendar so izolacijski testi negativni, ponovite izolacijske teste iz iste pelete ali tako, da vzamete dodatno žilno tkivo blizu stolona odrezanega gomolja istega vzorca, in po potrebi testirajte dodatne vzorce.
Scheme for detection and identification of Ralstonia solanacearum in samples of asymptomatic potato, tomato or other host plants
Shema za detekcijo in identifikacijo bakterije R. solanacearum v vzorcih asimptomatskih rastlin krompirja, paradižnika in drugih gostiteljskih rastlin
Sample of stem segments (1)
Vzorec delov stebel (1)
selective isolation (4) / IF test (5) / PCR test (6) / FISH test (7)
Selektivna izolacija (4) / IF test (5) / PCR test (6) / FISH test (7)
optional auxiliary test: ELISA (8), bioassay (9)
Dodatni pomožni test: ELISA (8), biološki test (9)
Colonies with typical morphology (10) after isolation
Kolonije z značilno morfologijo (10) po izolaciji
All tests done negative
Vsi opravljeni testi negativni
Sample not infected by
Vzorec ni okužen z
Sample infected by
Vzorec je okužen z
See Section III.2.1. for recommended sample sizes.
Glej oddelek III.2.1 za priporočene velikosti vzorcev.
Pathogen extraction and concentration methods are described in Section III.2.1.
Metode ekstrakcije in koncentracije patogena so opisane v oddelku III.2.1.
If at least two tests based on different biological priciples are positive, isolation and confirmation have to be done Perform at least one screening test.
Če sta pozitivna vsaj dva testa, ki temeljita na različnih bioloških načelih, je treba opraviti izolacijo in potrditev. Opravite vsaj en presejalni test.
The IF test is described in Section VI.A.5.
IF test je opisan v oddelku VI.A.5.
If positive results are obtained in screening tests, but the isolation tests are negative, then repeat the isolation tests.
Če pri presejalnih testih dobite pozitivne rezultate, vendar so izolacijski testi negativni, ponovite izolacijske teste.
Reliable identification of pure presumptive R. solanacearum cultures is achieved using the tests described in Section VI.B.
Zanesljivo identifikacijo čistih kultur domnevne R. solanacearum dosežemo s testi, opisanimi v oddelku VI.B.
DETAILED METHODS FOR DETECTION OF RALSTONIA SOLANACEARUM IN POTATO TUBERS AND POTATO, TOMATO OR OTHER HOST PLANTS WITH SYMPTOMS OF BROWN ROT OR BACTERIAL WILT
PODROBNE METODE ZA DETEKCIJO BAKTERIJE R. SOLANACEARUM V GOMOLJIH KROMPIRJA TER NA RASTLINAH KROMPIRJA, PARADIŽNIKA IN DRUGIH GOSTITELJSKIH RASTLINAH S SIMPTOMI RJAVE GNILOBE ALI BAKTERIJSKEGA VENENJA
Symptoms (see website http://forum.europa.eu.int/Public/irc/sanco/Home/main)
Simptomi (glej spletno mesto http://forum.europa.eu.int/Public/irc/sanco/Home/main)
Symptoms on potato
Simptomi na krompirju
The potato plant.
Rastlina krompirja.
The early stage of infection in the field is recognised by wilting of the leaves towards the top of the plant at high temperatures during the day with recovery at night.
Zgodnja stopnja okužbe se kaže kot venenje listov proti vrhu rastline pri visokih dnevnih temperaturah, ponoči pa si rastlina opomore.
In early stages of wilting leaves remain green, but later yellowing and brown necrosis develops.
V zgodnjih stopnjah venenja listi ostanejo zeleni, kasneje pa začnejo rumeneti in razvije se rjava nekroza.
Epinasty also occurs.
Pojavi se tudi epinastija.
Wilting of one shoot or whole plants becomes rapidly irreversible and results in the collapse and death of the plant.
Venenje enega poganjka ali cele rastline hitro postane nepovratno in se konča s sesedanjem in z odmrtjem rastline.
The vascular tissue of transversely cut stems from wilted plants usually appears brown and a milky bacterial ooze exudes from the cut surface or can be expressed by squeezing.
Žilno tkivo prečno prerezanih stebel uvelih rastlin je običajno rjavo, na površini prereza pa je mogoče opaziti ali z lahkoto iztisniti mlečni bakterijski izcedek.
When a cut stem is placed vertically in water, threads of slime will stream from the vascular bundles.
Če prerezano steblo postavimo navpično v vodo, iz žilnih snopov pritečejo sluzaste niti.
The potato tuber.
Gomolj krompirja.
Potato tubers must be cut transversely close to the heel (stolon end) or longitudinally over the stolon end.
Gomolje krompirja je treba prečno prerezati blizu območja stolona ali po dolžini čez stolon.
The early stage of infection is recognised by a glassy yellow to light brown discolouration of the vascular ring from which a pale cream bacterial ooze emerges spontaneously after some minutes.
Zgodnja stopnja okužbe se kaže kot steklasto rumeno do svetlo rjavo razbarvanje žilnega obroča, iz katerega se po nekaj minutah spontano pocedi bled, kremast bakterijski izcedek.
Later, the vascular discolouration becomes a more distinct brown and necrosis can extend into the parenchymatous tissue.
Pozneje postane razbarvanost žilnega tkiva bolj izrazito rjava, nekroza pa lahko zajame parenhimatično tkivo.
In advanced stages, infection breaks outwards from the heel end and the eyes from which bacterial slime may ooze causing soil particles to adhere.
V napredovalih stopnjah okužba izbruhne navzven od stolona in očes, iz katerih lahko mezi bakterijski izcedek, na katerega se začnejo lepiti grudice zemlje.
Reddish-brown slightly sunken lesions may appear on the skin due to collapse of vascular tissues internally.
Lahko se pojavijo rdečkasto-rjave, nekoliko udrte razpoke na povrhnjici zaradi notranjega odmrtja žilnih tkiv.
Secondary development of fungal and bacterial soft rots is common in the advanced stages of the disease.
V napredovalih stopnjah bolezni je značilen sekundarni razvoj glivičnih in bakterijskih mehkih gnilob.
Symptoms on tomato
Simptomi na paradižniku
The tomato plant.
Rastlina paradižnika.
The first visible symptom is the flaccid appearance of the youngest leaves.
Prvi vidni simptom je uvel videz najmlajših listov.
Under favourable environmental conditions for the pathogen (soil temperatures of approximately 25 °C; saturated humidity), epinasty and wilting of one side or of the whole plant follows within a few days leading to total plant collapse.
V ugodnih okoljskih pogojih za patogen (temperatura tal okrog 25 °C, nasičenost z vlago) sledita v nekaj dneh epinastija in venenje ene strani ali cele rastline, kar pripelje do njenega popolnega odmrtja.
Under less favourable conditions (soil temperature below 21 °C), less wilting occurs, but large numbers of adventitious roots may develop on the stem.
V manj ugodnih pogojih (temperatura tal pod 21 °C) je venenja manj, vendar lahko iz stebla požene večje število adventivnih korenin.
It is possible to observe watersoaked streaks from the base of the stem which is evidence of necrosis in the vascular system.
Mogoče je opaziti z vodo zasičene proge vzdolž stebla, ki so dokaz nekroze žilnega sistema.
When the stem is cut crosswise, discoloured brown vascular tissues exude white or yellowish bacterial ooze.
Če steblo prečno prerežemo, iz razbarvanega rjavega žilnega tkiva stebla pritečejo kaplje belega ali rumenkastega bakterijskega izcedka.
Symptoms on other hosts
Simptomi na drugih gostiteljih
Solanum dulcamara and S. nigrum plants.
Rastline Solanum dulcumara in S. nigrum.
Under natural conditions, wilting symptoms are rarely observed in these weed hosts unless soil temperatures exceed 25 °C or inoculum levels are extremely high (e.g. as for S. nigrum growing adjacent to diseased potato or tomato plants).
V naravnih pogojih na teh plevelnih gostiteljih simptome venenja redko opazimo, razen če temperatura tal presega 25 °C ali če je raven inokuluma izredno visoka (npr. če S. nigrum raste zraven bolnih rastlin krompirja ali paradižnika).
When wilting does occur, the symptoms are as described for tomato.
Ko pride do venenja, so simptomi enaki kot pri paradižniku.
Non-wilting S. dulcamara plants growing with stems and roots in water may show internal light brown discolouration of vascular tissues on transverse section of the stem base or underwater stem parts.
Rastline S. dulcamara, ki ne venejo in ki rastejo s steblom in koreninami v vodi, lahko v notranjosti razvijejo rahlo rjavo obarvanost žilnih tkiv na prečnem delu dna stebla ali na potopljenem delu stebla.
Bacteria may ooze from cut vascular tissues or form threads of slime if the cut stem is placed vertically in water, even in the absence of wilting symptoms.
Iz prerezanih žilnih tkiv lahko priteče bakterijski izcedek, oziroma če postavimo prerezano steblo navpično v vodo, nastanejo sluzaste niti, tudi če ni simptomov venenja.
Rapid screening tests may facilitate presumptive diagnosis but are not essential.
Hitri presejalni testi pospešijo domnevno diagnozo, vendar niso bistveni.
Use one or more of the following validated tests:
Uporabite enega ali več od spodnjih potrjenih testov:
(See Section VI.A.1.)
(glej oddelek VI.A.1)
Detection of poly-ß-hydroxybutyrate (PHB) granules
Detekcija zrnc poli-β-hidroksibutirata (PHB)
Characteristic PHB granules in the cells of R. solanacearum are visualised by staining heat-fixed smears of bacterial ooze from infected tissue on a microscope slide with Nile Blue A or Sudan Black (See Section VI.A.2.).
Značilna zrnca PHB v celicah R. solanacearum postanejo vidna po obarvanju toplotno fiksiranih madežev bakterijskega izcedka iz okuženega tkiva na stekelcu mikroskopa z nilsko modrim A ali sudan črnim (glej oddelek VI.A.2).
(See Section VI.A.3.)
(glej oddelek VI.A.3)
Other tests
Drugi testi
Further appropriate rapid screening tests include the IF test (see Section VI.A.5.), FISH test (see Section VI.A.7.), ELISA tests (see Section VI.A.8.) and PCR tests (see Section VI.A.6).
Drugi ustrezni hitri presejalni testi so IF-test (oddelek VI.A.5), test FISH (oddelek VI.A.7), testi ELISA (oddelek VI.A.8) in testi PCR (oddelek VI.A.6).
Remove ooze or sections of discoloured tissue from the vascular ring in the potato tuber or from the vascular strands in stems of potato, tomato or other wilting host plants.
Odvzamemo izcedek ali dele razbarvanega tkiva z žilnega obroča v gomolju krompirja ali z žilnih trakov v steblu rastlin krompirja, paradižnika ali drugih venečih gostiteljskih rastlin.
Suspend in a small volume of sterile distilled water or 50mM phosphate buffer (Appendix 4) and leave for 5 to 10 minutes.
Suspendiramo v majhni količini sterilne destilirane vode ali 50 mM fosfatnega pufra (Dodatek 4) in pustimo 5–10 minut.
Prepare a series of decimal dilutions of the suspension.
Pripravimo niz desetkratnih razredčin suspenzije.
Transfer 50-100 µl of the suspension and dilutions to a general nutrient medium (NA, YPGA or SPA; see Appendix 2) and/or to Kelman’s tetrazolium medium (Appendix 2) and/or a validated selective medium (e.g. SMSA; see Appendix 2).
50-100 µl suspenzije in razredčin prenesemo na splošno hranilno gojišče (NA, YPGA ali SPA, Dodatek 2) in/ali tetrazolno gojišče po Kelmanu (npr. SMSA, glej Dodatek 2).
Spread or streak with an appropriate dilution plating technique.
Razmažemo ali načrtamo z ustrezno tehniko razmaza razredčine.
If useful, prepare separate plates with a diluted cell suspension of R. solanacearum biovar 2 as a positive control.
Če se zdi uporabno, pripravimo posamezne plošče z razredčeno celično suspenzijo bakterije R. solanacearum biovar 2/biotip 3 za pozitivno kontrolo.
Incubate the plates for two to six days at 28 °C.
Plošče 2–6 dni inkubiramo pri 28 °C.
On the general nutrient media, virulent isolates of R. solanacearum develop pearly cream-white, flat, irregular and fluidal colonies often with characteristic whorls in the centre.
Na splošnem hranilnem gojišču virulentni izolati bakterije R. solanacearum tvorijo biserno kremno bele, ploske, nepravilne in tekoče kolonije, ki imajo v sredini pogosto značilne spirale.
Avirulent forms of R. solanacearum form small round non-fluidal, butyrous colonies which are entirely cream-white.
Nevirulentne oblike bakterije R. solanacearum tvorijo majhne, okrogle, netekoče, maslaste kolonije, ki so povsem smetanaste barve.
On Kelman’s tetrazolium and SMSA media, the whorls are blood red in colour.
Na tetrazolnem gojišču po Kelmanu in SMSA gojiščih so spirale krvavo rdeče.
Avirulent forms of Ralstonia solanacearum form small round non-fluidal, butyrous colonies which are entirely deep red.
Nevirulentne oblike bakterije R. solanacearum tvorijo majhne, okrogle, netekoče, maslaste kolonije, ki so povsem temno rdeče.
Tests to confirm identity of presumptive isolates of R. solanacearum are shown in Section VI.B.
Testi za potrjevanje identitete domnevnih izolatov bakterije R. solanacearum so prikazani v oddelku VI.B.
Detailed methods for detection and identification of Ralstonia solanacearum in samples of asymptomatic potato tubers
Podrobne metode za detekcijo in identifikacijo bakterije R. solanacearum v vzorcih asimptomatskih krompirjevih gomoljev
Larger numbers of tubers in the sample will lead to inhibition or difficult interpretation of the results.
Večje število gomoljev v vzorcu bo privedlo do inhibicije in otežilo razlago rezultatov.
However, the procedure can be conveniently applied for samples with less than 200 tubers where fewer tubers are available.
Postopek pa lahko prikladno uporabite za vzorce z manj kot 200 gomolji, če je na voljo manj gomoljev.
Validation of all detection methods described below is based on testing of samples of 200 tubers.
Potrjevanje vseh metod detekcije, ki so opisane spodaj, temelji na testiranju vzorcev z 200 gomolji.
The potato extract described below can also be used for detection of the potato ring rot bacterium, Clavibacter michiganensis subsp. sepedonicus.
Krompirjev izvleček, ki je opisan spodaj, lahko uporabite tudi za detekcijo bakterije krompirjeve obročkaste gnilobe, Clavibacter michiganensis podv. sepedonicus.
Optional pre-treatment in advance to sample preparation:
Dovoljena je tudi vnaprejšnja obdelava še pred pripravo vzorca.
Incubation of samples at 25 to 30 °C, for up to two weeks before testing, to encourage multiplication of any R. solanacearum populations.
Vzorec inkubiramo pri 25–30 °C do dva tedna pred testiranjem, da spodbudimo množenje majhnih populacij bakterije R. solanacearum.
Wash the tubers.
Gomolje operemo.
Use appropriate disinfectants (chlorine compounds when PCR-test is to be used in order to remove pathogen DNA) and detergents between each sample.
Med vsakim vzorcem uporabimo ustrezna razkužila (klorove spojine, kadar izvajamo test PCR, da odstranimo patogeno DNK) in detergente.
This washing procedure is particularly useful (but not required) for samples with excess soil and if a PCR-test or direct isolation procedure is to be performed.
Postopek pranja je še posebej koristen (vendar ni obvezen) za vzorce z odvečno zemljo in če bomo izvajali test PCR ali neposredno izolacijo.
Remove with a clean and disinfected scalpel or vegetable knife the skin at the heel (stolon) end of each tuber so that the vascular tissues become visible.
S čistim in sterilnim skalpelom ali nožem za zelenjavo odstranimo povrhnjico gomolja na območju stolona, tako da najprej postanejo vidna žilna tkiva.
Note: Set aside any (rotting) tubers with suspected brown rot symptoms and test separately.
Opomba: Če so v vzorcu (gnijoči) sumljivi gomolji s simptomi rjave gnilobe, jih umaknemo in jih testiramo posebej.
All tubers including those with suspicioussymptoms should be kept according to Annex III.
Vse gomolje, vključno tiste s sumljivimi simptomi, je treba hraniti v skladu s Prilogo III.
Collect the heel end cores in unused disposable containers which can be closed and/or sealed (in case containers are reused they should be thoroughly cleaned and disinfected using chlorine compounds).
Jedra stolona zberemo v neuporabljene posode, ki jih je mogoče zapreti in/ali hermetično zapreti (če so posode večkrat uporabljene, jih je najprej treba temeljito očistiti in razkužiti s klorovimi spojinami).
Preferably, the heel end cores should be processed immediately.
Zaželeno je, da se stoloni obdelajo takoj.
If this is not possible, store them in the container, without addition of buffer, refrigerated for not longer than 72 hours or for not longer than 24 hours at room temperature.
Če to ni možno, jih shranimo v posodo brez dodatka pufra, v hladilniku za največ 72 ur oziroma pri sobni temperaturi ne dlje kot 24 ur.
Process the heel end cores by one of the following procedures: either,
Jedra stolonov obdelamo po enem od naslednjih postopkov, in sicer bodisi tako, da:
Cover the cores with sufficient volume (approximately 40 ml) of extraction buffer (Appendix 4) and agitate on a rotary shaker (50-100 rpm) for 4 hours below 24 °C or for 16 to 24 hours refrigerated,
dodamo zadostno količino (pribl. 40 ml) ekstrakcijskega pufra (Dodatek 4) in stresamo na rotacijskem stresalniku (50-100 vrt/min) 4 ure pod temperaturo 24 °C ali 16–24 ur pri temperaturi hladilnika;