natgillin/translations-raw huggingface dataset:
If an electrical blender is used, the blender must be operated three to four times for approximately one second each time.
Če se uporabi električni mešalec, ga je treba vključiti tri- do štirikrat, tako da vsakič deluje približno po eno sekundo.
Digestion procedure
Postopek prebave
This procedure may involve complete pools (100 g of samples at a time) or pools of less than 100 g.
Postopek lahko vključuje popolne skupke (100 g vzorcev hkrati) ali skupke manj kot 100 g.
Complete pools (100 samples at a time)
Popolni skupki (100 vzorcev hkrati)
The stomacher lab-blender 3500 is fitted with a double plastic bag and the temperature control set at 40 to 41 oC.
Mešalec Stomacher Lab 3500 je opremljen z dvojno plastično vrečko, temperaturno kazalo pa je nastavljeno na 40 do 41 oC.
One and a half litres of water preheated to 40 to 41 oC is poured into the inner plastic bag.
V notranjo plastično vrečko se vlije liter in pol vode, segrete na 40 do 41 oC.
25 ml of 17,5 % hydrochloric acid is added to the water in the stomacher.
Vodi v mešalcu Stomacher se doda 25 ml 17,5 % klorovodikove kisline.
100 samples weighing approximately 1 g each (at 25 to 30 oC) taken from each individual sample in accordance with 2 are added.
Nato se doda 100 vzorcev po približno 1 g (pri 25 do 30 oC), ki so bili vzeti od posameznih vzorcev v skladu s točko 2.
Lastly, 6 g pepsin is added.
Nazadnje se doda 6 g pepsina.
This order must be followed strictly to avoid decomposition of the pepsin.
Ta vrstni red je treba strogo upoštevati, da se prepreči razkroj pepsina.
The stomacher is then allowed to pound the content of the bag for 25 minutes.
Stomacher nato 25 minut melje vsebino vrečke.
The plastic bag is removed from the stomacher and the digestion fluid is filtered through the sieve into a 3 litre beaker.
Plastična vrečka se odstrani iz Stomacherja, digestivna tekočina pa se prefiltrira skozi sito v 3-litrsko čašo.
The plastic bag is washed with approximately 100 ml of water, which is then used to rinse the sieve and lastly added to the filtrate in the beaker.
Plastična vrečka se splakne s približno 100 ml vode, s katero se nato splakne sito in nazadnje doda filtratu v čašo.
Up to 15 individual samples can be added to a total pool of 100 samples and examined together with these samples.
K skupku 100 vzorcev se lahko doda do 15 posameznih vzorcev, ki se pregledajo skupaj s temi vzorci.
Smaller pools (less than 100 samples)
Manjši skupki (manj kot 100 vzorcev)
A digestion fluid is prepared by mixing about one and a half litres of water and 25 ml of 17,5 % hydrochloric acid.
Digestivno tekočino se pripravi tako, da se zmeša en liter in pol vode ter 25 ml 17,5 % klorovodikove kisline.
6 g of pepsin is added and the whole mixed at a temperature of 40 to 41 oC.
Doda se 6 g pepsina in vse skupaj zmeša pri temperaturi med 40 in 41 oC.
Of the digestion fluid, a volume corresponding to 15 ml per gram of sample is measured (e.g. for 30 samples the volume required is 30 × 15 ml = 450 ml) and transferred to the inner of the two plastic bags, together with the meat samples weighing approximately 1 g (at 25 to 30 oC) taken from each individual sample in accordance with 2.
Od digestivne tekočine se odmeri količina, ki ustreza 15 ml na gram vzorca (npr. za 30 vzorcev je potrebna količina 30 x 15 ml = 450 ml), ki se doda v obe plastični vrečki skupaj z vzorci mesa po približno 1 g (pri 25 do 30 oC), vzetih od posameznih vzorcev v skladu s točko 2.
Water at a temperature of approximately 41 oC is poured into the outer bag to make up a total volume in the two bags of one and a half litres.
Voda pri temperaturi približno 41 oC se vlije v zunanjo vrečko, dokler v obeh vrečkah skupna prostornina ne znaša en liter in pol.
The plastic bag is washed with approximately 100 ml of water (at 25 to 30 oC), which is then used to rinse the sieve and lastly added to the filtrate in the beaker.
Plastična vrečka se splakne s približno 100 ml vode (pri 25 do 30 oC), s katero se nato splakne sito in nazadnje doda filtratu v čašo.
Recovery of larvae by sedimentation
Ugotavljanje prisotnosti ličink s sedimentacijo
Ice (300 to 400 g of ice flakes, scaly ice or crushed ice) is added to the digestion fluid to bring its volume up to about 2 litres.
Digestivni tekočini se doda led (300 do 400 g ledenih ploščic, ledenega praška ali zdrobljenega ledu), ki njen volumen poveča na približno 2 litra.
The digestion fluid is then stirred until the ice has melted.
Digestivna tekočina se nato meša, dokler se led ne raztopi.
In the case of smaller pools (see II(b)), the amount of ice must be reduced correspondingly.
Pri manjših skupkih (glej II(b)) je treba količino ledu ustrezno zmanjšati.
The chilled digestion fluid is transferred to a 2 litre separation funnel, equipped with a vibrator in an extra clamp.
Ohlajena digestivna tekočina se prenese v dvolitrski ločilni lijak, ki je opremljen z vibratorjem, pritrjenim s posebno objemko.
Sedimentation is allowed to proceed for 30 minutes, during which time the sedimentation funnel is vibrated intermittently, i.e. one minute vibration followed by a one-minute pause.
Sedimentacija lahko poteka 30 minut, medtem pa sedimentacijski lijak prekinjeno vibrira, tj. enominutna vibracija, kateri sledi enominutni premor.
After 30 minutes, a 60 ml sample of the sediment is quickly run off into a 100 ml measuring cylinder (the funnel is rinsed with detergent solution after use).
Po 30 minutah se 60 ml vzorec sedimenta hitro odplakne v 100 ml merilni valj. (Lijak se po uporabi splakne z raztopino detergenta).
The 60 ml sample is allowed to stand for at least 10 minutes, after which time the supernatant is withdrawn by suction to leave a volume of 15 ml, to be examined for presence of larvae.
60 ml vzorec se pusti stati najmanj 10 minut, nato se supernatant odvzame s pipetiranjem, da znaša preostala prostornina 15 ml, ki se pregleda na prisotnost ličink.
For suction, a disposable syringe, equipped with a plastic tube, can be used.
Za pipetiranje se lahko uporabi brizgalka za enkratno uporabo, opremljena s plastično cevko.
The length of the tube must be such that 15 ml remains in the measuring cylinder when the flanges of the syringe rest on the cylinder's rim.
Dolžina cevke mora biti tolikšna, da 15 ml ostane v merilnem valju, ko po pipetiranju brizgalka ostane na obroču valja.
The remaining 15 ml is poured into a larval counting basin or two petri dishes and examined using a trichinoscope or stereo-microscope.
Preostalih 15 ml se odlije v posodo za štetje ličink ali dve petrijevki ter pregleda s trihinoskopom ali stereomikroskopom.
The measuring cylinder is washed with 5 to 10 ml of tap water and the washings are added to the sample.
Merilni valj se splakne z 5 do 10 ml vode iz pipe, to pa se doda k vzorcu.
Under no circumstances is examination to be postponed until the following day.
V nobenem primeru se pregled ne sme prestaviti na naslednji dan.
Where the digests are unclear or they are not examined within 30 minutes of their preparation, they must be clarified as follows:
Če so vzorci motni ali pa niso bili pregledani v 30 minutah po njihovi pripravi, jih je treba razbistriti na naslednji način:
the final sample of 60 ml is poured into a measuring cylinder and allowed to stand for 10 minutes; 45 ml of supernatant fluid is then removed by suction and the remaining 15 ml is made up to 45 ml with tap water,
končni vzorec 60 ml se vlije v merilni valj in pusti stati 10 minut; nato se 45 ml supernatanta (bistre tekočine) odvzame s pipetiranjem, preostalim 15 ml pa se dolije voda iz pipe do 45 ml,
after a further settling period of 10 minutes, 30 ml of supernatant fluid is removed by suction and the remaining 15 ml is poured into a petri dish or larval counting basin for examination,
po nadaljnjem usedanju, ki traja 10 minut, se 30 ml supernatanta (bistre tekočine) odvzame s pipetiranjem, preostalih 15 ml za pregled pa se vlije v petrijevko ali posodo za štetje ličink,
the measuring cylinder is washed with 10 ml of tap water and these washings are added to the sample in the petri dish or the larval counting basin for examination.
merilni valj se splakne z 10 ml vode iz pipe, to pa se skupaj doda k vzorcu v petrijevki ali posodi za štetje ličink, ki je pripravljen za pregled.
Where the result is positive or uncertain, the provisions laid down in Chapter I(3)(III) shall apply.
Če je rezultat pozitiven ali nejasen, se uporabljajo določbe iz poglavja I(3)(III).
Mechanically assisted pooled sample digestion method/‘on filter isolation’ technique
Mehanska metoda prebave zbirnega vzorca/tehnika „izolacije na filtru“
As stipulated in Chapter II(A)(1).
Kot je določeno v poglavju II(A)(1).
1 litre Gelman funnel, complete with filter holder (diameter 45 mm);
1-litrski Gelman lijak skupaj z držalom za filter (premer 45 mm);
filter discs, consisting of a circular stainless steel mesh with an aperture of 35 microns (disc diameter: 45 mm), two rubber rings 1 mm thick (external diameter: 45 mm; internal diameter: 38 mm), the circular mesh being placed between the two rubber rings and bonded to them using a two-component glue suitable for the two materials;
filtrirni diski, ki so sestavljeni iz okrogle mreže iz nerjavečega jekla z odprtino 35 mikronov (premer diska: 45 mm), dveh gumijastih obročev, debelih 1 mm, (zunanji premer: 45 mm, notranji premer: 38 mm), okrogla mreža se položi med oba gumijasta obroča in nanju pritrdi z uporabo dvokomponentnega lepila, ki je primerno za oba materiala;
an Erlenmeyer flask, capacity 3 litres, fitted with a side tube for suction;
erlenmajerica s prostornino treh litrov, ki je opremljena s stransko cevko za pipetiranje;
a filter pump;
filtrirna črpalka;
plastic bags, capacity at least 80 ml;
plastične vrečke s prostornino najmanj 80 ml;
equipment for sealing the plastic bags;
oprema za zapiranje plastičnih vrečk;
rennilase, strength 1: 150000 soxhlet units per gram.
rennilase z močjo 1: 150000 soxhlet enot na gram.
Collecting of specimens
Odvzem vzorcev
See Chapter II(A)(3)(II)(a).
Glej poglavje II(A)(3)(II)(a).
See Chapter II(A)(3)(II)(b).
Glej poglavje II(A)(3)(II)(b).
Recovery of larvae by filtration
Ugotavljanje prisotnosti ličink s filtracijo
In the case of smaller pools, the amount of ice must be reduced correspondingly.
Pri manjših skupkih je treba količino ledu ustrezno zmanjšati.
The digestion fluid is stirred until the ice has melted.
Digestivna tekočina se meša, dokler se led ne raztopi.
The chilled digestion fluid is then left for at least three minutes to let the larvae coil.
Ohlajeno digestivno tekočino se nato pusti stati najmanj tri minute, da se ličinke zvijejo.
The Gelman funnel, fitted with a filter holder and filter disc, is mounted on an Erlenmeyer flask connected to a filter pump.
Gelman lijak, opremljen z držalom za filter in filtrirnim diskom, se pritrdi na erlenmajerico, ki je povezana s filtrirno črpalko.
The digestion fluid is poured into the Gelman funnel and filtered.
Digestivna tekočina se vlije v Gelman lijak in prefiltrira.
Towards the end of filtration, the digestion fluid can be helped to pass through the filter by applying suction with the filter pump.
Proti koncu filtracije se lahko pretok digestivne tekočine skozi filter olajša z uporabo filtrirne črpalke.
Suction must cease before the filter becomes dry, i.e. when 2 to 5 ml of fluid is left in the funnel.
Pipetiranje se mora zaključiti, preden se filter osuši, to pomeni, ko v lijaku ostane 2 do 5 ml tekočine.
Once all the digestion fluid has been filtered, the filter disc is removed and placed in an 80 ml capacity plastic bag, together with 15 to 20 ml of rennilase solution.
Ko je vsa digestivna tekočina prefiltrirana, se filtrirni disk odstrani in položi v plastično vrečko s prostornino 80 ml skupaj s 15 do 20 ml rennilase raztopine.
The rennilase solution is made by adding 2 g of rennilase to 100 ml of tap water.
Rennilase raztopina se naredi tako, da se 2 g rennilase doda k 100 ml vode iz pipe.
The plastic bag is sealed twice and placed between the inner and outer bags in the stomacher.
Plastična vrečka se dvakrat zapečati in vloži v Stomacher med notranjo in zunanjo vrečko.
The stomacher is allowed to pound for three minutes, e.g. while it is working on a complete or incomplete pool.
Stomacher naj melje tri minute, npr. popoln ali nepopoln vzorec.
After three minutes, the plastic bag, complete with filter disc and rennilase solution, is removed from the stomacher and opened with scissors.
Po treh minutah se plastična vrečka skupaj s filtrirnim diskom in rennilase raztopino odstrani iz Stomacherja in odpre s škarjami.
The liquid contents are poured into a larval counting basin or petri dish.
Tekoča vsebina se vlije v posodo za štetje ličink ali petrijevko.
The bag is washed out with 5 to 10 ml of water, which is then added to the larval counting basin for examination by trichinoscope or to the petri dish for examination by stereo-microscope.
Vrečka se splakne s 5 do 10 ml vode, ki se nato doda v posodo za štetje ličink za pregled pod trihinoskopom ali v petrijevko za pregled s stereomikroskopom.
Digests must be examined as soon as they are ready.
Vzorci se morajo pregledati takoj, ko so pripravljeni.
Filter discs must never be used when not completely clean.
Filtrirni diski se ne smejo uporabljati, če niso popolnoma čisti.
Unclean discs must never be allowed to dry out.
Diski se nikoli ne smejo posušiti v nečistem stanju.
Filter discs can be cleaned by leaving them in rennilase solution overnight.
Filtrirni diski se lahko očistijo tako, da se čez noč pustijo v rennilase raztopini.
Before use, they must be washed in fresh rennilase solution using the stomacher.
Pred uporabo jih je treba očistiti v sveži rennilase raztopini z uporabo Stomacherja.
Automatic digestion method for pooled samples of up to 35 g
Avtomatska metoda umetne prebave zbirnih vzorcev do 35 g
A Trichomatic 35® blender with filtration insert
mešalec Trichomatic 35® s filtrskim vložkom;
Hydrochloric acid 8,5 ± 0,5 % weight
klorovodikova kislina z 8,5 % ± 0,5 % maso;
Transparent polycarbonate membrane filters with a diameter of 50 mm and a pore size of 14 microns
prozorni polikarbonatni membranski filtri s premerom 50 mm in velikostjo pore 14 mikronov;
Pepsin, strength 1: 10000 NF (US National Formulary) corresponding to 1: 12500 BP (British Pharmacopoeia) and to 2000 FIP (Fédération internationale de pharmacie)
pepsin z močjo 1: 10000 NF (US National Formulary), kar ustreza 1: 125000 BP (British Pharmacopoea) in 2000 FIP (Fédération Internationale de Pharmacie);
A balance accurate to 0,1 g
tehtnica z natančnostjo do 0,1 g;
Tweezers with a flat tip
pinceta s ploščato konico;
A number of microscope slides with a side-length of at least 5 cm or a number of petri dishes at least 6 cm in diameter, marked on their undersides into 10 × 10 mm square areas using a pointed instrument
objektna stekelca za mikroskopiranje z dolžino stranice najmanj 5 cm ali petrijevke s premerom najmanj 6 cm, katerih dno je s koničastim instrumentom razdeljeno na kvadrate velikosti 10 x 10 mm;
A (stereo-)microscope with transmitted light (magnification 15 to 60 times) or a trichinoscope with a horizontal table
nastavljiv (stereo-) mikroskop (15 do 60-kratna povečava) ali trihinoskop s horizontalno ploščo;
A bin for collection of waste liquids
posoda za zbiranje tekočih odpadkov;
a thermometer accurate to 0,5 oC within the range 1 to 100 oC.
termometer z natančnostjo do 0,5 oC v obsegu od 1 do 100 oC.
I. Digestion procedure
I. Postopek prebave
Place the blender with the filtration insert, connect the waste tube and place the tube so it drains into the waste bin.
Namestite mešalec s filtrirnim vložkom, povežite odvodno cevko in jo napeljite v posodo za zbiranje tekočih odpadkov.
When the blender is switched on, heating will start.
Po vključitvi mešalca se začne segrevanje.
Before this is done, the bottom valve located below the reaction chamber must be opened and closed.
Pred tem je treba odpreti in zapreti spodnji ventil, ki se nahaja pod reakcijsko komoro.
Up to 35 samples weighing approximately 1 g each (at 25 to 30 oC) taken from each individual sample in accordance with point 2 are then added.
Nato se doda največ 35 vzorcev po približno 1 g (pri 25 do 30 oC), ki so bili vzeti od posameznih vzorcev v skladu s točko 2.
Ensure that larger pieces of tendons are removed as they may clot the membrane filter.
Preverite, da so odstranjeni večji kosi kit, ker lahko zamašijo membranski filter.
Pour water up to the edge of a liquid chamber connected to the blender (approximately 400 ml).
Nalijte vodo do roba komore za tekočino, povezane z mešalcem (približno 400 ml).
Pour about 30 ml hydrochloric acid ((8,5 %) to the edge of the smaller, connected liquid chamber.
Nalijte okrog 30 ml klorovodikove kisline (8,5 %) do roba manjše komore za tekočino, ki je povezana z mešalcem.
Place a membrane filter under the coarse filter in the filter holder in the filter insert.
Pod grobi filter v držalu filtrirnega vložka namestite membranski filter.
Lastly, add 7 g of pepsin.
Na koncu dodajte 7 g pepsina.
Close the lids of the reaction and liquid chambers.
Zaprite pokrova reakcijske komore in komore za tekočino.
Select the period of digestion.
Nastavite čas prebave.
A short digestion period (5 minutes) must be set for pigs at the normal slaughter age and a longer time (8 minutes) for other samples.
Kratek čas prebave (5 minut) je treba nastaviti za prašiče običajne starosti za zakol in podaljšani čas prebave (8 minut) za druge vzorce.
When the start button on the blender is turned on, the process of dispensing and digestion starts automatically, followed by filtration After 10 to 13 minutes the process is completed and stops automatically.
Z vklopom gumba za start na mešalcu se avtomatsko začne postopek dispenzacije in prebave, nato sledi filtriranje. Po 10 do 13 minutah je postopek končan in mešalec se avtomatsko ustavi.
Open the lid of the reaction chamber after checking that the chamber is empty.
Ko ste preverili, ali je reakcijska komora prazna, odprite njen pokrov.
If there is foam or any digestion liquid remaining in the chamber, repeat the procedure in accordance with V.
Če so v komori pena ali ostanki digestivne tekočine, ponovite postopek v skladu s točko V.
Recovery of larvae
Ugotavljanje prisotnosti ličink
Remove the filter holder and transfer the membrane filter to a slide or Petri dish.
Odstranite držalo filtra in prenesite membranski filter na objektno stekelce ali petrijevko.
Examine the membrane filter using a (stereo-) microscope or a trichinoscope.
Preglejte membranski filter s (stereo) mikroskopom ali trihinoskopom.
Cleaning equipment
Čiščenje opreme
Where the result is positive, fill the blender reaction chamber with boiling water until it is two-thirds full.
Če je rezultat pozitiven, napolnite 2/3 reakcijske komore v mešalcu z vrelo vodo.
Ordinary tap water is poured into the connecting liquid chamber until it covers the lower sensor.
Komoro za tekočino napolnite z vodo iz pipe do ravni spodnjega kazalca.
Automatic cleaning then takes place.
Nato se začne avtomatsko čiščenje.
Decontaminate the filter-holder and any other equipment, e.g. using formol.
Razkužite držalo filtra in preostalo opremo, na primer z uporabo formalina.
After work is completed for the day, fill the blender liquid chamber with water and put it through a standard cycle.
Pred koncem delavnika napolnite komoro za tekočino v mešalcu z vodo in izvedite standardni program.
Use of membrane filters
Uporaba membranskih filtrov
Each polycarbonate membrane filter may be used no more than five times.
Vsak polikarbonatni membranski filter se lahko uporabi največ petkrat.
The filter is to be turned between each use.
Filter je treba po vsaki uporabi obrniti.
In addition, the filter must be checked after each use for any damage which would make it unsuitable for further use.
Poleg tega je treba po vsaki uporabi preveriti, ali je filter morda poškodovan, zaradi česar ne bi bil več primeren za nadaljnjo uporabo.
V. Method to be applied when digestion is incomplete and filtration cannot be carried out
V. Metoda, ki se uporabi, kadar prebava ni popolna in zato ni mogoče opraviti filtracije
Once the blender has been put through an automatic cycle in accordance with C(3)(I), open the lid of the reaction chamber and check whether there is foam or any liquid remaining in the chamber.
Ko ste v mešalcu izvedli avtomatski postopek v skladu s točko C(3)(I), odprite pokrov reakcijske komore in preverite, ali je v komori ostala pena ali tekočina.
If this is the case, proceed as follows:
Če je tako, opravite naslednji postopek:
close the bottom valve below the reaction chamber;
Zaprite spodnji ventil pod reakcijsko komoro.
remove the filter holder and transfer the membrane filter to a slide or Petri dish;
Odstranite držalo filtra in prenesite membranski filter na objektno stekelce ali petrijevko.
put a new membrane filter in the filter holder and attach the filter holder;
Vložite nov membranski filter v držalo filtra in pritrdite držalo filtra.
fill the blender liquid chamber with water until the lower sensor is covered;
Komoro mešalca za tekočino napolnite z vodo do ravni spodnjega kazalca.
carry out the automatic cleaning cycle;
Izvedite avtomatski program čiščenja.
after the cleaning cycle has ended, open the lid of the reaction chamber and check whether any liquid remains;
Ko je program čiščenja končan, odprite pokrov reakcijske komore in preverite, ali je v komori še kaj tekočine.
if the chamber is empty, remove the filter holder and transfer the membrane filter to a slide or Petri dish with tweezers;
Če je komora prazna, odstranite držalo filtra in s pinceto prenesite membranski filter na objektno stekelce ali petrijevko.
examine the two membrane filters in accordance with C(3)(II).
Oba membranska filtra preglejte v skladu s točko C(3)(II).
If the filters cannot be examined, repeat the entire digestion process with a longer digestion time in accordance with C(3)(I).
Če filtrov ni mogoče pregledati, ponovite celoten postopek prebave s podaljšanim časom prebave v skladu s točko C(3)(I).
TRICHINOSCOPIC EXAMINATION
TRIHINOSKOPSKE PREISKAVE
An incandescent-lamp trichinoscope with 30 to 40 times and 80 to 100 times magnification or a stereomicroscope with a substage transmitted light source of adjustable intensity
Trihinoskop z žarnico s 30- do 40-kratno in 80- do 100-kratno povečavo ali stereomikroskop z nastavljivim svetlobnim virom;
A compressorium being a pressure glass consisting of two glass plates (one of which is divided into equal fields)
kompresorij, tj. stiskalo, sestavljeno iz dveh steklenih plošč (od katerih je ena razdeljena na enaka polja);
Small curved scissors
majhne zakrivljene škarje;
Small forceps
majhne klešče;
A knife for cutting specimens
nož za rezanje vzorcev;
Small numbered containers for storing the specimens separately
oštevilčene posodice za ločeno hranjenje vzorcev;
A dropping pipette
kapalka;
A glass of acetic acid and a glass of potassium hydroxide solution for brightening any calcifications and softening dried meat.
kozarec ocetne kisline in kozarec raztopine kalijevega hidroksida za razbistritev nastalih poapnitev ali mehčanje suhega mesa.
In the case of whole carcases, several hazelnut-size samples are taken from each animal:
Pri celih trupih je treba od vsake živali vzeti več vzorcev v velikosti lešnika:
in domestic swine, such samples are taken from both diaphragm pillars at the transition of the sinewy part;
Pri domačih prašičih se takšni vzorci vzamejo iz obeh preponskih stebrov pri prehodu v mišični del.
in wild boar samples are taken from both diaphragm pillars at the transition of the sinewy part and in addition from the jaw, the muscles of the lower leg, the intercostal muscles and the tongue muscles, giving a total of six samples from each individual animal;
Pri divjih merjascih se vzorci vzamejo iz obeh preponskih stebrov pri prehodu v mišični del, poleg tega pa tudi iz čeljusti, mišic spodnjega dela noge, medrebrnih mišic in jezika, kar je skupaj šest vzorcev od vsake posamezne živali.
if certain muscles are not available for sampling, a total of four samples are taken from the muscles that are available;
Če določene mišice niso na voljo za vzorčenje, se skupaj vzame štiri vzorce iz mišic, ki so na voljo.