natgillin/translations-raw huggingface dataset:
The following negative controls should be included in the PCR test:
V test PCR se morajo vključiti naslednje negativne kontrole:
buffer controls used for extracting the bacterium and the DNA from the sample,
Kontrole pufrov, ki se uporabljajo za ekstrakcijo bakterije in DNK iz vzorca.
PCR-reaction mix.
Reakcijska zmes PCR.
The following positive controls should be included:
Morajo se vključiti naslednje pozitivne kontrole:
aliquots of resuspended pellets to which C. m. subsp. sepedonicus has been added (preparation see Appendix 2),
Alikvoti resuspendiranih pelet, ki jim je bila dodana C. m. subsp. sepedonicus (za pripravo glej Dodatek 2).
a suspension of 106 cells per ml of C. m. subsp. sepedonicus in water from a virulent isolate (e.g. NCPPB 2140 or NCPPB 4053),
Suspenzija 106 celic na ml C. m. subsp. sepedonicus v vodi iz virulentnega izolata (npr. NCPPB 2140 ali NCPPB 4053).
if possible also use DNA extracted from positive control samples in the PCR test.
Po možnosti se uporabi tudi DNKA, izvlečen iz pozitivnih kontrolnih vzorcev v testu PCR.
To avoid potential contamination prepare positive controls in a separate environment from samples to be tested.
Da se ognemo potencialni okužbi, pripravimo pozitivne kontrole v okolju, ločenem od vzorcev, ki jih nameravamo testirati.
Sample extracts should be as free as possible from soil.
Na ekstraktih vzorca mora biti čim manj zemlje.
It could therefore in certain cases advisible to prepare extractions from washed potatoes if PCR protocols are to be used.
Zato bi bilo v nekaterih primerih priporočljivo pripraviti ekstrakte iz umitih krompirjev, če se uporabijo protokoli PCR.
DNA purification methods
Metode prečiščevanja DNK
Use positive and negative control samples as described above.
Uporabimo vzorce pozitivne in negativne kontrole, kot je opisano zgoraj.
Prepare control material in an identical manner as the sample(s).
Kontrolni material pripravimo na enak način kot vzorec ali vzorce.
A variety of methods are available for purification of target DNA from complex sample substrates, thus removing inhibitors of PCR and other enzymatic reactions and concentrating target DNA in the sample extract.
Na voljo je več metod prečiščevanja ciljne DNK iz kompleksnih substratov vzorca, pri čimer se odstrani inhibitorje PCR in druge encimske reakcije in v ekstraktu vzorca skoncentrira ciljna DNK.
The following method has been optimised for use with the validated PCR method shown in Appendix 6.
Naslednja metoda je bila optimizirana za uporabo s potrjeno metodo PCR, prikazano v Dodatku 6.
6.1.(a) Method according to Pastrik (2000)
6.1(a) Pastrikova metoda (2000):
Pipette 220 µl of lysis buffer (100 mM NaCl, 10 mM Tris-HCl [pH 8,0], 1 mM EDTA [pH 8,0]) into a 1,5 ml Eppendorf tube.
S pipeto kanemo 220 µl lizirnega pufra (100 mM NaCl, 10 mM Tris-HCl [pH 8,0], 1 mM EDTA [pH 8,0]) v 1,5 ml Eppendorfovo epruveto.
Add 100 µl sample extract and place in a heating block or waterbath at 95 °C for 10 minutes.
Dodamo 100 µl ekstrakta vzorca in položimo na grelno površino ali v vodno kopel pri 95 °C za 10 minut.
Put tube on ice for five minutes.
Za 5 minut položimo epruveto na led.
Add 80 µl Lysozyme stock solution (50 mg lysozyme per ml in 10 mM Tris HCl, pH 8,0) and incubate at 37 °C for 30 minutes.
Dodamo 80 µl osnovne raztopine lizocima (50 mg lizocima na ml v 10 mM Tris HCl, pH 8,0) in 30 minut inkubiramo pri 37 °C.
Add 220 µl of Easy DNA® solution A (Invitrogen), mix well by vortexing and incubate at 65 °C for 30 minutes.
Dodamo 220 µl Easy DNA® raztopine A (Invitrogen), dobro premešamo z vrtinčenjem in 30 minut inkubiramo pri 65 °C.
Add 100 µl of Easy DNA® solution B (Invitrogen), vortex vigorously until the precipitate runs freely in the tube and the sample is uniformly viscous.
Dodamo 100 µl Easy DNA® raztopine B (Invitrogen), močno premešamo z vrtinčenjem, da oborina prosto plava v epruveti in je vzorec enotno viskozen.
Add 500 µl of chloroform and vortex until the viscosity decreases and the mixture is homogeneous.
Dodamo 500 µl kloroforma in premešamo z vrtinčenjem, dokler se viskoznost ne zmanjša in zmes ne postane homogena.
Centrifuge at 15000 g for 20 minutes at 4 °C to separate phases and form the interphase.
20 minut centrifugiramo pri 15000 g pri 4 °C, da ločimo faze in oblikujemo interfazo.
Transfer the upper phase into a fresh Eppendorf tube.
Zgornjo fazo prenesemo v svežo Eppendorfovo epruveto.
Add 1 ml of 100 % ethanol (–20 °C) vortex briefly and incubate on ice for 10 minutes.
Dodamo 1 ml 100 % etanola (–20 °C), na kratko premešamo z vrtinčenjem in 10 minut inkubiramo na ledu.
Centrifuge at 15000 g for 20 minutes at 4 °C and remove ethanol from pellet.
10 minut centrifugiramo pri 15000 g pri 4 °C in odstranimo etanol iz pelete.
Add 500 µl 80 % ethanol (–20 °C) and mix by inverting the tube.
Dodamo 500 µl 80 % etanola (–20 °C) in premešamo tako, da obračamo epruveto.
Centrifuge at 15000 g for 10 minutes at 4 °C, save the pellet and remove ethanol.
10 minut centrifugiramo pri 15000 g pri 4 °C, ohranimo peleto in odstranimo etanol.
Allow the pellet to dry in air or in a DNA speed vac.
Pustimo, da se peleta posuši na zraku ali v vakumski centrifugi.
Resuspend the pellet in 100 µl sterile UPW and leave at room temperature for at least 20 minutes.
Resuspendiramo peleto v 100 µl sterilne UPW in vsaj 20 minut pustimo na sobni temperaturi.
Store at –20 °C until required for PCR.
Shranimo pri –20 °C dokler je ne potrebujemo za PCR.
Spin down any white precipitate by centrifugation and use 5 µl of the supernatant containing DNA for the PCR.
S centrifugiranjem odvrtimo vso belo oborino in uporabimo 5 µl supernatanta, ki vsebuje DNK za PCR.
Prepare test and control templates for PCR according to the validated protocol (Appendix 6).
Pripravimo testne in kontrolne matrice za PCR v skladu z potrjenim protokolom (Dodatek 6).
Prepare one decimal dilution of sample DNA extract (1:10 in UPW).
Pripravimo desetkratno razredčino ekstrakta vzorca DNK (1:10 v UPW).
Prepare the appropriate PCR reaction mix in a contamination-free environment according to the published protocol (Appendix 6).
Pripravimo ustrezno reakcijsko zmes PCR v neokuženem okolju v skladu z objavljenim protokolom (Dodatek 6).
The validated PCR protocol is a multiplex reaction that also incorporates an internal PCR control.
Potrjeni protokol PCR je multipla reakcija, ki vključuje tudi notranjo kontrolo PCR.
Add 5 µl of DNA extract per 25 µl PCR reaction in sterile PCR tubes.
Dodamo 5 µl ekstrakta DNK na 25 µl reakcijske zmesi PCR v sterilne epruvete PCR.
Incorporate a negative control sample containing only PCR reaction mix and add the same source of UPW as used in the PCR mix in place of sample.
Vključimo vzorec negativne kontrole, ki vsebuje samo reakcijsko zmes PCR, in dodamo isti vir UPW, kot je bil uporabljen v zmesi PCR pri vzorcu.
Place tubes in the same thermal cycler which was used in preliminary testing and run the appropriately optimised PCR programme (Appendix 6).
Namestimo epruvete v isti ciklični termostat, kot je bil uporabljen pri predhodnem testiranju, in zaženemo ustrezno optimiziran program PCR. (Dodatek 6).
Analysis of the PCR product
Analiza produkta PCR
Resolve PCR amplicons by agarose gel electrophoresis.
Odkrijemo pomnožke PCR z elektroforezo v agaroznem gelu.
Run at least 12 µl of amplified DNA reaction mixture from each sample mixed with 3 µl loading buffer (Appendix 6) in 2,0 % (w/v) agarose gels in tris-acetate-EDTA (TAE) buffer (Appendix 6) at 5 to 8 V per cm.
Odlijemo vsaj 12 µl pomnožene reakcijske zmesi DNK iz vsakega vzorca, zmešane s 3 µl nanašalnega pufra (Dodatek 6) v 2,0 % (w/v) agarozne gele v tris-acetat-EDTA (TAE) pufru (Dodatek 6) pri 5–8 V na cm.
Use an appropriate DNA marker, e.g. 100 bp ladder.
Uporabimo ustrezni označevalec DNK, npr. lestvico 100 bp.
Reveal DNA bands by staining in ethidium bromide (0,5 mg per L) for 30 to 45 min taking appropriate precautions for handling this mutagen.
Razkrijemo proge DNK z obarvanjem v etidijevem bromidu (0,5 mg na L) 30 do 45 minut, pri čemer se ustrezno zavarujemo pri ravnanju s to mutageno snovjo.
Observe stained gel under short wave UV transillumination (e.g. λ = 302 nm) for amplified PCR products of the expected size (Appendix 6) and document.
Opazujemo obarvani gel pod kratkovalovno UV presvetlitvijo. (npr. λ = 302 nm) in iščemo pomnožene produkte PCR pričakovane velikosti (Dodatek 6) ter dokumentiramo.
For all new findings/cases verify authenticity of the PCR amplicon by performing restriction enzyme analysis on a sample of the remaining amplified DNA by incubating at the optimum temperature and time with an appropriate enzyme and buffer (see Appendix 6).
Za vse nove ugotovitve/primere preverimo avtentičnost pomnožka PCR, tako da opravimo analizo restrikcije encima na vzorcu preostale pomnožene DNK z inkubacijo pri optimalni temperaturi in času z ustreznim encimom in pufrom (glej Dodatek 6).
Resolve the digested fragments by agarose gel electrophoresis as before and observe characteristic restriction fragment pattern under UV transillumination after ethidium bromide staining and compare with the undigested and digested positive control.
Odkrijemo raztopljene delce z elektroforezo v agaroznem gelu kot prej in opazujemo značilne vzorce restrikcijskih delcev pod UV presvetlitvijo po obarvanju z etidijevim bromidom in primerjamo z neraztopljeno in raztopljeno pozitivno kontrolo.
Interpretation of the PCR test result:
Interpretacija rezultatov testa PCR:
The PCR test is negative if the C. m. subsp. sepedonicus-specific PCR amplicon of expected size is not detected for the sample in question but is detected for all positive control samples (in case of multiplex PCR with plant specific internal control primers: a second PCR-product of expected size must be amplified with the sample in question).
Test PCR je negativen, če v zadevnem vzorcu ne opazimo za C. m. subsp. sepedonicus značilnega pomnožka PCR pričakovane velikosti, pojavi pa se v vseh pozitivnih kontrolnih vzorcih (v primeru multple PCR z za rastlino značilnimi začetniki za notranje kontrole: drugi produkt PCR se mora povečati pri zadevnem vzorcu).
The PCR test is positive if the C. m. subsp. sepedonicus-specific PCR amplicon of expected size and restriction pattern (when required) is detected, providing that it is not amplified from any of the negative control samples.
Test PCR je pozitiven, če odkrijemo za C. m. subsp. sepedonicus značilen pomnožek PCR pričakovane velikosti in vzorca restrikcije (če je potrebno), pod pogojem, da ni pomnožen iz nobenega negativnega kontrolnega vzorca. Zanesljiva potrditev pozitivnega rezultata se lahko pridobi s ponovitvijo testa z drugim nizom začetnikov PCR.
Reliable confirmation of a positive result can also be obtained by repeating the test with a second set of PCR primers (section 9.3).
(oddelek 9.3).
Inhibition of the PCR may be suspected if the expected amplicon is obtained from the positive control sample containing C. m. subsp. sepedonicus in water but negative results are obtained from positive controls with C. m. subsp. sepedonicus in potato extract.
Inhibicija PCR se lahko pričakuje, če se pričakovani pomnožek pridobi iz vzorca pozitivne kontrole, ki vsebuje C. m. subsp. sepedonicus v vodi, vendar so negativni rezultati pridobljeni iz pozitivnih kontrol C. m. subsp. sepedonicus v ekstraktih krompirja.
In multiplex PCR protocols with internal PCR controls, inhibition of the reaction is indicated when neither of the two amplicons are obtained.
V multiplih protokolih PCR z notranjo kontrolo PCR kaže na inhibicijo reakcije, kadar ni pridobljen nobeden od obeh pomnožkov.
Contamination may be suspected if the expected amplicon is obtained from one or more of the negative controls.
Na okužbo se sumi, če je pričakovani pomnožek pridobljen iz ene ali več negativnih kontrol.
BIOASSAY TEST
BIOLOŠKI TEST
Preliminary testing with this method should permit reproducible detection of 103 to 104 colony-forming units of C. m. subsp. sepedonicus per ml added to sample extracts that previously tested negative (preparation see Appendix 2).
Predhodno testiranje s to metodo mora omogočati ponovljivo odkrivanje vsaj 103 do 104 enot C. m. subsp. sepedonicus, ki ustvarjajo kolonije na ml, dodano ekstraktom vzorcev, ki so bili prej testirani z negativnim rezultatom (za pripravo glej Dodatek 2).
Highest sensitivity of detection can be expected when using freshly prepared sample extract and optimal growth conditions.
Največjo občutljivost pri odkrivanju lahko pričakujemo pri uporabi sveže pripravljenih ekstraktov vzorca in v optimalnih rastnih pogojih.
However, the method can be successfully applied to extracts that have been stored under glycerol at –68 to –86 °C.
Vendar pa se metoda lahko uspešno uporabi tudi pri ekstraktih, ki so bili shranjeni v glicerolu pri –68 do –86 °C.
Some varieties of eggplant provide an excellent selective enrichment medium for the growth of C. m. subsp. sepedonicus even in the absence of symptoms and also provide an excellent confirmatory host test.
Nekatere sorte jajčevca so odlično obogatitveno gojišče za rast C. m. subsp. sepedonicus tudi kadar ni simptomov in so prav tako tudi odličen potrditveni gostiteljski test.
Growth conditions should be optimal to reduce the risk of false negative test results.
Rastni pogoji morajo biti optimalni, tako da se zmanjša nevarnost napačnih negativnih rezultatov teta.
For cultural details, see Appendix 8.
Za podrobnosti o kulturi glej Dodatek 8.
Distribute the whole of the remaining test aliquot of the resuspended pellet from section 3.1.6 or 3.2.5 between eggplants by one of the methods given below (7.3 or 7.4).
Med jajčevce porazdelimo ves preostali testni alikvot resuspendirane pelete iz oddelka 3.1.6 ali 3.2.5, z uporabo ene od spodnjih metod (7.3 ali 7.4).
Use only plants at leaf stage two to three up to full expansion of the third true leaf.
Uporabimo samo rastline v stadiju olistanosti 2–3 do popolne razvitosti tretjega pravega lista.
In order to ensure complete utilisation of the resuspended pellet as well as effective inoculation the procedures outlined below will require 15 to 25 eggplants per sample.
Da bi zagotovili popolno uporabo resuspendirane pelete, kakor tudi učinkovito inokulacijo, se za spodnje postopke zahteva 15–25 jajčevcev na vzorec.
Do not water eggplants for one to two days prior to inoculation to reduce turgor pressure.
1 do 2 dni pred inokulacijo ne zalivamo jajčevcev, da se zmanjša turgor.
Slit inoculation
Inokulacija z zarezo
Holding the plant between two fingers, pipette a drop (approximately 5 to 10 µl) of the suspended pellet on the stem between the cotyledons and the first leaf.
Ko držimo rastlino med dvema prstoma, s pipeto kanemo kapljico (približno 5 do 10 µl) suspendirane pelete na steblo med kličnima lista in prvim listom.
Using a sterile scalpel, make a diagonal slit, about 1,0 cm long and approximately 2/3 of the stem thickness deep, starting the cut from the pellet drop.
S sterilnim skalpelom diagonalno zarežemo od peletne kapljice naprej, pri čemer je zareza dolga približno 1,0 cm in globoka 2/3 debeline stebla.
Seal the cut with sterile vaseline from a syringe.
Zarezo zapremo s sterilnim vazelinom iz brizgalke.
Syringe inoculation
Inakulacija z brizgalko
Inoculate the eggplant stems just above the cotyledons using a syringe fitted with a hypodermic needle (not less than 23 G).
Inakuliramo steblo jajčevca tik nad kličnim listom z uporabo brizgalke, ki ima hipodermično iglo (ne manj kot 23G).
Distribute the sample between the eggplants.
Porazdelimo vzorec med jajčevce.
As the positive controls, inoculate 5 plants with an aqueous suspension of 105 to 106 cells per ml of a known culture of C. m. subsp. sepedonicus and, where possible, with naturally infected tuber tissue (see section 4) by the same inoculation method (7.3 or 7.4).
Za pozitivno kontrolo inokuliramo 5 rastlin z vodno raztopino 105 do 106 celic na ml znane kulture C. m. subsp. sepedonicus in, kadar je mogoče, z naravno okuženim tkivom gomolja (glej Oddelek 4) z isto inokulacijsko metodo (7.3 ali 7.4).
As the negative control, inoculate 5 plants with sterile pellet buffer by the same inoculation method (7.3 or 7.4).
Za negativno kontrolo inokuliramo 5 rastlin s sterilnim peletnim pufrom z isto inokulacijsko metodo (7.3 ali 7.4).
Incubate plants in quarantine facilities for up to four weeks at 18 to 24 °C.
Inkubiramo rastline v karantenskih prostorih do 4 tedne pri 18 do 24 °C.
Incubate plants with sufficient light and high humidity (70 to 80 %) and water to prevent water logging or wilting through water deficiency.
Inkubiramo rastline z zadostno svetlobo in visoko vlažnostjo (70–80 %) ter zalivamo, da preprečimo nalaganje vode ali venenje.
sepedonicus cells are killed at temperatures above 30 °C and the optimum temperature is 21 °C.
Celice C. m. sepedonicus umrejo pri temperaturi nad 30 °C, optimalna temperatura pa je 21 °C.
To avoid contamination incubate positive control and negative control plants on clearly separated benches in a glasshouse or growth chamber or, in case space is limited, ensure strict separation between treatments.
Da se izognemo okužbi, inkubiramo rastline pozitivne in negativne kontrole na jasno ločenih klopeh v rastlinjaku ali rastnem prostoru ali v primeru omejenega prostora zagotovimo strogo ločitev med obravnavanjem.
If plants for different samples must be incubated close together, separate them with appropriate screens.
Če morajo biti rastline za različne vzorce inkubirane tesno skupaj, jih ločimo z ustreznimi zasloni.
When fertilising, watering, inspecting and any other manipulations take great care to avoid cross-contamination.
Pri gnojenju, zalivanju, preverjanju in drugih posegih moramo močno paziti da ne pride do navzkrižne okužbe.
It is essential to keep glasshouses and growth chambers free of all insect pests since they may transmit the bacterium from sample to sample.
Bistvenega pomena je, da v rastlinjaku ali rastnem prostoru ni insektov, saj ti lahko prenesejo bakterije iz vzorca na vzorec.
Examine regularly for symptoms starting after a week.
Po enem tednu redno preverjamo simptome.
Count the number of plants showing symptoms.
Preštejemo število rastlin, ki kažejo simptome.
subsp. sepedonicus causes leaf wilting in eggplants which may commence as a marginal or interveinal flaccidity.
subsp. sepedonicus povzroča venenje listov jajčevcev, kar se lahko začne kot obrobna ali medžilna mlahavost.
Wilted tissue may initially appear dark green or mottled but turns paler before becoming necrotic.
Ovenelo tkivo je lahko sprva temno zeleno ali lisasto, vendar postane bledo preden postane nekrotično.
Interveinal wilts often have a greasy water-soaked appearance.
Medžilno venenje ima pogosto masten, razmočen videz.
Necrotic tissue often has a bright yellow margin.
Nekrotično tkivo ima pogosto svetlo rumen rob.
Plants are not necessarily killed; the longer the period before symptoms develop, the greater the chance of survival.
Ni nujno, da rastline odmrejo; čim kasneje se simptomi razvijejo, večja je možnost preživetja.
Plants may outgrow the infection.
Rastline lahko okužbo prebolijo.
Young eggplants are much more susceptible to low populations of C. m. subsp. sepedonicus than are older plants, hence the necessity to use plants at or just before leaf stage 3.
Mlade rastline so veliko bolj dovzetne za manjše populacije C. m. subsp. sepedonicus kot starejše rastline, zato je potrebno uporabiti rastline na ali stadijem olistanosti 3.
Wilts may also be induced by populations of other bacteria or fungi present in the tuber tissue pellet.
Do venenja lahko pride tudi zaradi populacij drugih bakterij ali gliv v peleti gomoljnjega tkiva.
These include Ralstonia solanacearum, Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica, Erwinia chrysanthemi, Phoma exigua var. foveata, as well as large populations of saprophytic bacteria.
Sem so vključene Ralstonia solanacearum, Erwinia carotovora subsp. carotovora in E. carotovora subsp. atroseptica, Erwinia chrysanthemi, Phoma exigua var. foveata, kakor tudi velike populacije saprofitskih bakterij.
In particular Erwinia chrysanthemi can cause leaf symptoms and wilt that is very similar to symptoms of C. m. sepedonicus.
Zlasti Erwinia chrysanthemi lahko povzroči listne simptome in venenje, ki so zelo podobni simptomom C. m. sepedonicus.
The only difference is blackening of the stems in case in Erwinia chrysanthemi infections.
Edina razlika je črnenje stebel pri okužbah z Erwinia chrysanthemi.
Other wilts can be distinguished from those caused by C. m. subsp. sepedonicus since whole leaves or whole plants wilt rapidly.
Drugo venenje je moč razlikovati od tistega, ki ga povzroča C. m. subsp. sepedonicus, saj celi listi ali cela rastlina hitro venijo.
Also a Gram stain can be prepared: this test will differentiate C. m. subsp. sepedonicus from Erwinia spp.
Lahko se pripravi tudi obarvanje po Gramu: s tem testom razločimo med C. m. subsp. sepedonicus in Erwinia spp.
As soon as symptoms in eggplants are observed reisolation should be performed, using sections of wilted leaf tissue or stem tissue from plants (see 3.1. 3 for tissue maceration).
Kakor hitro pri jajčevcih opazimo simptome, je treba opraviti ponovno izolacijo, z uporabo delov ovenelega listnega tkiva ali stebelnega tkiva od rastlin (glej 3.1.3 za maceracijo tkiva).
Surface disinfect the eggplant leaves and stems by wiping with 70 % ethanol.
Površinsko razkužimo liste in stebla jajčevca, tako da jih obrišemo s 70 % etanolom.
Perform an IF test or PCR on the eggplant sap and isolate on to suitable (selective) media (see section 8).
Opravimo test IF ali PCR na soku jajčevca in izoliramo na primerno (selektivno) gojišče (glej oddelek 8).
A Gram stain (Appendix 9) may also be prepared.
Lahko se pripravi tudi obarvanje po Gramu (Dodatek 9).
Identify purified cultures of presumptive C. m. subsp. sepedonicus and confirm pathogenicity (see section 9 and 10).
Identificiramo prečiščene kulture domnevne C. m. subsp. sepedonicus in potrdimo patogenost (glej oddelek 9 in 10).
Under certain circumstances, in particular where growing conditions are not optimal, it may be possible for C. m. subsp. sepedonicus to exist as a latent infection within eggplants even after incubation periods up to 4 weeks.
V nekaterih okoliščinah, zlasti kadar rastni pogoji niso optimalni, lahko C. m. subsp. sepedonicus obstaja kot latentna okužba v jajčevcih, tudi po izteku inkubacijskega obdobja do 4 tedne.
If no symptoms are observed after 4 weeks perform IF/PCR on a composite sample of 1 cm stem sections of each test plant taken above the inoculation site.
Če se simptomi po 4 tednih ne pojavijo, opravimo test IF/PCR na sestavljenem vzorcu 1 centimetrskih delcev stebla vsake testne rastline, ki se odvzame nad mestom inokulacije.
If the test is positive reisolation on suitable (selective) media should be performed following the procedure under section 8.
Če je test pozitiven, se mora opraviti ponovna izolacija na primernem (selektivnem) gojišču, po postopku iz oddelka 8.
Identify purified cultures of presumptive C. m. subsp. sepedonicus and confirm pathogenicity (section 9 and 10).
Identificiramo prečiščene kulture domnevne C. m. subsp. sepedonicus in potrdimo patogenost (glej oddelek 9 in 10).
Interpretation of the bioassay test result.
Interpretacija rezultatov biološkega testa
Valid Bioassay test results are obtained when plants of the positive control show typical symptoms, the bacteria can be reisolated from these plants and no symptoms are found on the negative controls.
Veljavne rezultate biološkega testa pridobimo, če rastline pozitivne kontrole kažejo značilne simptome, če se bakterija lahko ponovno izolira iz teh rastlin in če negativne kontrole ne kažejo nobenih simptomov.
The bioassay test is negative if test plants are not infected by C. m. subsp. sepedonicus, and provided that C. m. subsp. sepedonicus is detected in positive controls.
Biološki test je negativen, če testne rastline niso okužene z bakterijo C. m. subsp. sepedonicus, pod pogojem da smo v pozitivni kontroli odkrili bakterije C. m. subsp. sepedonicus.
The bioassay test is positive if the test plants are infected by C. m. subsp. sepedonicus.
Biološki test je pozitiven, če so testne rastline okužene z bakterijo C. m. subsp. sepedonicus.
ISOLATION OF C. M. SUBSP.
IZOLACIJA C. M. SUBSP.