natgillin/translations-raw huggingface dataset:
Observe for characteristic spontaneous streaming of threads of bacterial slime from the cut vascular bundles after a few minutes.
Po nekaj minutah lahko opazimo, da iz prerezanih žilnih snopov spontano pritečejo sluzaste niti bakterijskega izcedka.
Prepare a smear of bacterial ooze from infected tissue or from a 48-hour culture on YPGA or SPA medium (Appendix 2) on a microscope slide.
Pripravimo razmaz bakterijskega izcedka iz okuženega tkiva na objektnem stekelcu ali razmaz 48-urne kulture na YPGA ali SPA (Dodatek 2).
Prepare positive control smears of a biovar 2 strain of R. solanacearum and, if considered useful, a negative control smear of a known PHB negative sp.
Pripravimo razmaze seva biovar 2 bakterije R. solanacearum za pozitivno kontrolo in, če se zdi uporabno, razmaz znanega PHB negativnega seva za negativno kontrolo.
Allow to air dry and pass the lower surface of each slide rapidly above a flame to fix the smears.
Razmaze pustimo, da se posušijo. Spodnjo površino stekelca hitro povlečemo prek plamena, da se razmaz fiksira.
Stain preparation with either Nile Blue or Sudan Black and observe microscopically as described below:
Preparat obarvamo ali z nilsko modrim ali s sudan črnim, in opazujemo pod mikroskopom, kot je opisano spodaj:
Nile blue test:
Test z nilsko modrim:
Flood each slide with 1 % aquous solution of Nile Blue A and incubate for 10 minutes at 55 °C.
Fiksiran razmaz zalijemo z 1-odstotno vodno raztopino nilsko modrega A. Inkubiramo 10 minut pri 55 °C.
Drain off the staining solution.
Odcedimo raztopino barvila.
Wash briefly in gently running tap water.
Na hitro speremo pod majhnim curkom vodovodne vode.
Remove excess water with tissue paper.
Odstranimo odvečno vodo z vpojnim papirjem.
Flood the smear with 8 % aqueous acetic acid and incubate for one minute at ambient temperature.
Razmaz zalijemo z 8-odstotno vodno raztopino ocetne kisline. Inkubiramo 1 minuto pri sobni temperaturi.
Re-moisten with a drop of water and apply a coverslip.
Ponovno navlažimo s kapljo vode. Pokrijemo s krovnim stekelcem.
Examine the stained smear with an epifluorescence microscope at 450 nm under oil immersion at a magnification of 600 to 1000 using an oil- or water-immersion objective.
Obarvani razmaz opazujemo pod epifluorescenčnim mikroskopom pri 450 nm v imerzijskem olju pri povečavi 600–1000. Uporabljamo imerzijski objektiv za olje ali za vodo.
Observe for bright orange fluorescence of PHB granules.
Iščemo svetlo oranžna fluorescenčna zrnca PHB.
Also observe under transmitted normal light to ensure that the granules are intracellular and that cell morphology is typical of R. solanacearum.
Opazujemo tudi pri dnevni svetlobi, da se prepričamo, da so zrnca znotraj celic in da je morfologija celic značilna za bakterijo R. solanacearum.
Sudan Black test:
Test s sudan črnim:
Flood each slide with 0,3 % Sudan Black B solution in 70 % ethanol and incubate for 10 minutes at ambient temperature.
Fiksiran razmaz zalijemo z 0,3-odstotno raztopino sudan črnega B v 70-odstotnem etanolu. Inkubiramo 10 minut pri sobni temperaturi.
Drain off the staining solution and wash briefly in tap water, removing excess water with tissue paper.
Odcedimo raztopino barvila. Na hitro speremo pod tekočo vodo. Odstranimo odvečno vodo z vpojnim papirjem.
Dip the slides briefly in xylol and blot dry on tissue paper.
Razmaz na kratko potopimo v ksilol. Popivnamo z vpojnim papirjem.
Caution: Xylol is harmful, take necessary safety precautions and work in a fume cupboard.
Pozor: Ksilol je zdravju škodljiv. Upoštevajte varnostne ukrepe in delajte v digestoriju.
Flood the slides with 0,5 % (w/v) aqueous safranin and leave for 10 seconds at ambient temperature.
Razmaz zalijemo z 0,5-odstotno (w/v) vodno raztopino safranina in pustimo 10 sekund pri sobni temperaturi.
Caution: Safranin is harmful, take necessary safety precautions and work in a fume cupboard.
Pozor: Safranin je zdravju škodljiv. Upoštevajte varnostne ukrepe in delajte v digestoriju.
Wash in gently running tap water, blot dry on tissue paper and apply a coverslip.
Speremo pod majhnim curkom vodovodne vode. Popivnamo z vpojnim papirjem. Pokrijemo s krovnim stekelcem.
Examine stained smears with a light microscope using transmitted light under oil immersion at a magnification of 1000 using an oil-immersion objective.
Obarvani razmaz opazujemo pod svetlobnim mikroskopom s presevno svetlobo v imerzijskem olju pri povečavi 1000. Uporabljamo imerzijski objektiv za olje.
Observe for blue-black staining of PHB granules in cells of R. solanacearum with pink-stained cell walls.
Zrnca PHB v celicah bakterije R. solanacearum se obarvajo modro-črno. Celične stene se obarvajo rožnato.
Agglutination of R. solanacearum cells in bacterial ooze or symptomatic tissue extracts is best observed using validated antibodies (see Appendix 3) labelled with appropriate coloured markers such as red Staphylococcus aureus cells or coloured latex particles.
Aglutinacijo celic R. solanacearum v bakterijskem izcedku ali izvlečku simptomatskega tkiva najlažje opazujemo s potrjenimi protitelesi (glej Dodatek 3), označenimi z ustreznimi barvnimi oznakami, kot so npr. rdeče celice Staphylococcus aureus ali obarvani delci lateksa.
If using a commercially available kit (see Appendix 3), follow the manufacturers instructions.
Če uporabljamo komercialni pribor (glej Dodatek 3), sledimo navodilom proizvajalca.
Otherwise perform the following procedure:
Sicer sledimo naslednjemu postopku:
Mix drops of a suspension of labelled antibody and bacterial ooze (approximately 5 µl each) on windows of multiwell test slides.
Zmešamo kapljice suspenzije označenega protitelesa in bakterijskega izcedka (vsak približno 5 µl) na okencih objektnih stekelc z več vdolbinicami.
Prepare positive and negative controls using suspensions of R. solanacearum biovar 2 and a heterologous strain.
Pripravimo pozitivno in negativno kontrolo s suspenzijo R. solanacearum biovar 2 in heterolognega seva.
Observe for agglutination in positive samples after gentle mixing for 15 seconds.
Po rahlem mešanju, ki traja 15 sekund, opazujemo aglutinacijo v pozitivnih vzorcih.
Note: Before using this method for the first time, perform preliminary tests to ensure reproducible detection of 103 to 104 colony-forming units of R. solanacearum per ml added to extracts from samples which previously tested negative.
Opomba: Preden prvič uporabimo to metodo, opravimo predhodne teste za zagotovitev ponovljive detekcije 103 do 104 enot, ki tvorijo kolonije bakterije R. solanacearum na ml, dodane ekstraktom iz vzorcev, ki so bili prej v testih negativni. Uporabimo primerno potrjeno selektivno gojišče, npr. SMSA (prilagojeno po Elphinstone in sod.
Use an appropriately validated selective medium such as SMSA (as modified by Elphinstone et al., 1996; see Appendix 2).
1996, glej Dodatek 2).
Care is required to differentiate R. solanacearum from other bacteria able to develop colonies on the medium.
Potrebno je pozorno razlikovati med R. solanacearum in drugimi bakterijami, ki so sposobne na tem gojišču razviti kolonije.
Furthermore, colonies of R. solanacearum may show atypical morphology if plates are overcrowded or antagonistic bacteria are also present.
Kolonije R. solanacearum lahko izkazujejo tudi atipično morfologijo, če so plošče prenaseljene ali če so prisotne antagonistične bakterije.
Where effects of competition or antagonism are suspected, the sample should be re-tested using a different test.
Kjer sumimo na učinke tekmovanja ali antagonizma, moramo vzorec ponovno testirati z drugačnim testom.
Highest sensitivity of detection by this method can be expected when using freshly prepared sample extracts.
Največjo občutljivost detekcije s to metodo lahko pričakujemo, če uporabljamo sveže pripravljene ekstrakte vzorcev.
However, the method is also applicable for use with extracts which have been stored under glycerol at –68 to –86 °C.
Metoda pa je uporabna tudi za ekstrakte, ki so bili shranjeni pod glicerolom pri –68 do –86 °C.
As positive controls, prepare decimal dilutions from a suspension of 106 cfu per ml of a virulent biovar 2 strain of R. solanacearum (e.g. NCPPB 4156 = PD 2762 = CFBP 3857).
Za pozitivno kontrolo pripravimo desetkratne razredčine iz suspenzije 106 enot, ki tvorijo kolonije, na ml virulentnega biovarja 2 seva R. solanacearum (npr. NCPPB 4156 = PD 2762 = CFBP 3857).
To avoid any possibility of contamination, prepare positive controls totally separately from samples to be tested.
Da ne bi prišlo do kontaminacije, pripravimo pozitivne kontrole povsem ločeno od vzorcev, ki jih nameravamo testirati.
For each newly prepared batch of a selective medium its suitability for growth of the pathogen should be tested before it is used to test routine samples.
Za vsako novo pripravljeno serijo selektivnega gojišča je treba preveriti, ali je ustrezno za rast patogena, preden ga uporabimo za testiranje rutinskih vzorcev.
Test control material in an identical manner as the sample(s).
Kontrolni material testiramo povsem enako kot vzorce.
Perform an appropriate dilution plating technique aiming to ensure that any background saprophytic colony-forming populations are diluted out.
Izvedemo ustrezno tehniko redčitvenega razmaza z namenom, da zagotovimo razredčenje morebitnih populacij saprofitskih bakterij, ki tvorijo kolonije, v ozadju.
Spread 50 - 100 µl per plate of sample extract and each dilution.
Razmažemo 50-100 µl na ploščo ekstrakta vzorca in vsako razredčino.
Incubate plates at 28 °C.
Plošče inkubiramo pri 28 °C.
Read plates after 48 hours and daily thereafter up to six days.
Plošče odčitamo po 48 urah in do 6 dni zatem vsak dan.
Typical R. solanacearum colonies on SMSA medium are milky white, flat, irregular and fluidal and after three days incubation develop pink to blood-red coloration in the centre with internal streaking or whorling. (see website http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Tipične kolonije R. solanacearum na gojišču SMSA so mlečno bele, ploske, nepravilnih oblik in tekoče, in po treh dneh inkubacije razvijejo rožnato do krvavo rdečo obarvanost v sredini, z notranjimi progami ali spiralami (glej spletno mesto http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Note: Atypical colonies of R. solanacearum sometimes form on this medium.
Opomba: Na tem gojišču se včasih razvijejo atipične kolonije bakterije R. solanacearum.
These may be small, round, entirely red in colour and non-fluidal or only partially fluidal and therefore difficult to distinguish from saprophytic colony-forming bacteria.
Te so lahko majhne, okrogle, povsem rdeče barve in netekoče ali samo delno tekoče, zato jih je težko razlikovati od saprofitskih bakterij, ki tvorijo kolonije.
Purify presumptive R. solanacearum colonies after streaking or dilution plating onto a general nutrient medium to obtain isolated colonies (see Appendix 2).
Prečistimo domnevne kolonije R. solanacearum, potem ko smo jih načrtali ali redčitveno razmazali na splošno hranilno gojišče. Tako dobimo izolirane kolonije (glej Dodatek 2).
Store cultures short-term in sterile water (pH 6 to 8, chlorine free) at room temperature in the dark, or long term in a suitable cryoprotectant medium at –68 to –86 °C or lyophilised.
Kratkoročno shranimo kulture v sterilni vodi (pH 6-8, brez klora) pri sobni temperaturi v temi, dolgoročno pa v primernem kriogenskem zaščitnem sredstvu pri temperaturi –68 do –86 °C ali liofilizirane.
Identify presumptive cultures (see Section VI.B.) and perform a pathogenicity test (see Section VI.
Identificiramo domnevne kulture (glej oddelek VI. B) in opravimo test patogenosti (glej oddelek VI.
Interpretation of selective plating test results
Interpretacija testa z razmazom na selektivno gojišče
The selective plating test is negative if no bacterial colonies are observed after six days or if no presumptive colonies typical of R. solanacearum are found, provided that no inhibition is suspected due to competition or antagonism by other bacteria and that typical R. solanacearum colonies are found in the positive controls.
Test z razmazom na selektivno gojišče je negativen, če po šestih dneh ne opazimo nobenih bakterijskih kolonij ali nobenih domnevnih kolonij, značilnih za bakterije R. solanacearum, pod pogojem, da inhibicija s strani kolonij drugih bakterij ni verjetna in da smo pri pozitivni kontroli našli značilne kolonije bakterije R. solanacearum.
The selective plating test is positive if presumptive R. solanacearum colonies are isolated.
Test z razmazom na selektivno gojišče je pozitiven, če izoliramo kolonije, značilne za bakterije R. solanacearum.
Use a validated enrichment medium such as modified Wilbrink broth (see Appendix 2).
Uporabimo potrjeno sredstvo za obogatitev, npr. modificiran bujon Wilbrink (Dodatek 2).
This procedure can be used to selectively increase R. solanacearum populations in sample extracts and increase sensitivity of detection.
S tem postopkom lahko selektivno povečamo populacijo R. solanacearum v ekstraktih vzorcev in povečamo občutljivost detekcije.
The procedure also effectively dilutes inhibitors of the PCR reaction (1:100).
Postopek tudi učinkovito razredči inhibitorje reakcije PCR (1:100).
It should be noted, however, that enrichment of R. solanacearum can fail due to competition or antagonism by saprophytic organisms which are often simultaneously enriched.
Treba pa se je zavedati, da lahko obogatitev R. solanacearum spodleti zaradi tekmovanja in antagonizma saprofitskih organizmov, ki se pogosto hkrati obogatijo.
For this reason, isolation of R.solanacearum from enriched broth cultures may be difficult.
Zato je lahko izolacijaR. solanacearum iz obogatenih kultur bujonov težavna.
In addition, since populations of serologically related saprophytes can be increased, the use of specific monoclonal antibodies rather than polyclonal antibodies is recommended where the ELISA test is to be used.
Ker je populacije serološko povezanih saprofitov mogoče povečati, se priporoča tudi uporaba specifičnih monoklonskih protiteles namesto poliklonskih protiteles, če uporabljamo test ELISA.
For enrichment-PCR, transfer 100 µl of sample extract into 10 ml of enrichment broth (Appendix 2) previously aliquoted into DNA-free tubes or flasks.
Za obogatitveni PCR prenesemo 100 µl ekstrakta vzorca v 10 ml obogatitvenega bujona (Dodatek 2), ki smo ga prej alikvotirali v epruvete ali stekleničke brez DNK.
For enrichment-ELISA, higher proportions of sample extract to broth can be used (e.g. 100 µl in 1,0 ml of enrichment broth).
Za obogatitveni test ELISA lahko uporabimo večji delež ekstrakta vzorca v bujonu (npr. 100 µl v 1,0 ml obogatitvenega bujona).
Incubate for 72 hours at 27 to 30 °C in shaking culture or static culture with caps loosely- fitted to permit aeration.
Inkubiramo 72 ur pri 27 do 30 °C v stresalni ali statični kulturi, pri čemer so pokrovi rahlo zaprti, da omogočimo zračenje.
Mix well before using in ELISA or PCR tests.
Pred uporabo v testu ELISA ali PCR dobro premešamo.
Treat enriched broth in an identical manner as the sample(s) in the above tests.
Z obogatenim bujonom ravnamo enako kot z vzorci v zgornjih testih.
Note: If inhibition of enrichment of R. solanacearum is anticipated, due to high populations of certain competing saprophytic bacteria, enrichment of sample extracts before any centrifugation or other concentration steps may give better results.
Opomba: Če pričakujemo inhibicijo obogatitve R. solanacearum zaradi velikih populacij nekaterih tekmujočih saprofitskih bakterij, bomo boljše rezultate morda dobili, če ekstrakte vzorcev obogatimo pred centrifugiranjem oziroma pred drugimi postopki koncentriranja.
The use of the IF test as the principal screening test is recommended because of its proven robustness to achieve the required thresholds.
Uporaba IF testa kot glavnega presejalnega testa je priporočljiva, ker je dokazano obstojen pri doseganju zahtevanih pragov.
When the IF test is used as the principal screening test and the IF reading is positive, the Isolation, PCR or FISH test must be performed as a second screening test.
Če IF test uporabljamo kot glavni presejalni test in je odčitek IF pozitiven, je treba kot drugi presejalni test opraviti izolacijski test, test PCR ali FISH.
When the IF test is used as the second screening test and the IF reading is positive, further testing according to the flow scheme is required to complete the analysis.
Če IF test uporabljamo kot drugi presejalni test in je odčitek IF pozitiven, je za zaključek analize potrebno nadaljnje testiranje glede na diagram poteka.
Note: Use a validated source of antibodies to R. solanacearum (see web site http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Opomba: Uporabimo potrjen vir protiteles za R. solanacearum (glej spletno mesto http://forum.europa.eu.int/Public/irc/sanco/Home/main).
The titre is defined as the highest dilution at which optimum reaction occurs when testing a suspension containing 105 to 106 cells per ml of the homologous strain of R. solanacearum and using an appropriate fluorescein isothiocyanate (FITC) conjugate according to the manufacturer’s recommendations.
Titer je definiran kot najvišja razredčina, pri kateri pride do optimalne reakcije, če testiramo suspenzijo, ki vsebuje 105 do 106 celic na ml homolognega seva bakterije R. solanacearum, z ustrezno razredčino flourescein izotiocianatovega (FITC) konjugata v skladu z navodili proizvajalca.
Validated polyclonal antisera all had an IF titre of at least 1:2000.
Potrjeni poliklonalni antiserumi so imeli vsi titer IF vsaj 1:2000.
During testing, the antibodies should be used at a working dilution(s) close to or at the titre.
Med testiranjem je treba protitelesa uporabiti z delovno razredčino (razredčinami), ki je blizu ali enaka titru.
The test should be performed on freshly-prepared sample extracts.
Test je treba opraviti na sveže pripravljenih ekstraktih vzorcev.
If necessary, it can be successfully performed on extracts stored at –68 to –86 °C under glycerol.
Po potrebi ga je mogoče uspešno opraviti na ekstraktih, shranjenih pri –68 do –86 °C pod glicerolom.
Glycerol can be removed from the sample by addition of 1 ml pellet buffer (Appendix 4), re-centrifugation for 15 minutes at 7000 g and re-suspension in an equal volume of pellet buffer.
Glicerol odstranimo iz vzorca z dodatkom 1 ml peletnega pufra (Dodatek 4), ponovnim 15-minutnim centrifugiranjem pri 7000 g in resuspendiranjem v enakem volumnu peletnega pufra.
This is often not necessary, especially if samples are fixed to the slides by flaming.
To pogosto ni potrebno, še posebej če so vzorci na stekelcih fiksirani s plamenom.
Prepare separate positive control slides of the homologous strain or any other reference strain of R. solanacearum, suspended in potato extract, as specified in Appendix 3 B, and optionally in buffer.
Pripravimo ločena stekelca za pozitivno kontrolo s homolognim sevom ali katerimkoli drugim referenčnim sevom bakterije R. solanacearum, suspendiranim v ekstraktu krompirja, kot je navedeno v Dodatku 3 B, lahko pa tudi v pufru.
Naturally infected tissue (maintained by lyophilisation or freezing at –16 to –24 °C) should be used where possible as a similar control on the same slide.
Naravno okuženo tkivo (ohranjeno z liofilizacijo ali zamrzovanjem pri –16 do –24 °C) je treba uporabiti, kjer je mogoče, kot podobno kontrolo na istem stekelcu.
As negative controls, aliquots of sample extract which previously tested negative for R. solanacearum can be used.
Kot negativne kontrole lahko uporabimo alikvote ekstrakta vzorca, ki so bili prej testirani kot negativni za R. solanacearum.
Standardised positive and negative control materials available for use with this test are listed in Appendix 3.
Standardizirani pozitivni in negativni kontrolni materiali, ki so na voljo za uporabo s tem testom, so navedeni v Dodatku 3.
Use multiwell microscope slides with preferably 10 windows of at least 6 mm diameter.
Uporabimo objektna stekelca z več vdolbinicami, po možnosti z 10 okenci po vsaj 6 mm v premeru.
Prepare the test slides by one of the following procedures:
Testna stekelca pripravimo po enem od naslednjih postopkov:
For pellets with relatively little starch sediment:
Za pelete z relativno malo škroba:
Pipette a measured standard volume (15 µl is appropriate for 6 mm window diameter – scale up volume for larger windows) of a 1/100 dilution of the resuspended potato pellet onto the first window.
V prvo okence odpipetiramo standardno količino (za okence s premerom 6 mm ustreza 15 µl –za večja okenca količino povečamo) razredčine 1/100 resuspendirane krompirjeve pelete.
Subsequently pipette a similar volume of undiluted pellet (1/1) onto the remaining windows on the row.
Nato odpipetiramo podobno količino nerazredčene pelete (1/1) na preostala okenca v vrstici.
Prepare decimal dilutions (1/10, 1/100) of the resuspended pellet in pellet buffer.
Pripravimo desetkratne razredčine (1/10, 1/100) resuspendirane pelete v peletnem pufru.
Pipette a measured standard volume (15 µl is appropriate for 6 mm window diameter – scale up volume for larger windows) of the resuspended pellet and each dilution on a row of windows.
V eno vrstico okenc odpipetiramo standardno količino (za okence s premerom 6 mm ustreza 15 µl –za večja okenca količino povečamo) resuspendirane pelete in vsake posamezne razredčine.
Dry the droplets at ambient temperature or by warming to temperatures of 40 to 45 °C.
Počakamo, da se kapljice posušijo pri sobni temperaturi, ali jih segrejemo do temperature 40-45 °C.
Fix the bacterial cells to the slide either by heating (15 minutes at 60 °C), flaming, with 95 % ethanol or according to specific instructions from the suppliers of the antibodies.
Celice bakterij fiksiramo na stekelce bodisi s segrevanjem (15 minut pri 60 °C), plamenom, 95-odstotnim etanolom, ali po specifičnih navodilih dobaviteljev protiteles.
If necessary, fixed slides may then be stored frozen in a desiccated box for as little time as necessary (up to a maximum of three months) prior to further testing.
Fiksirana stekelca lahko po potrebi shranimo zamrznjena v sušilni škatli, čim manj časa je potrebno (največ 3 mesece), preden jih spet testiramo.
IF procedure
Postopek IF
According to test slide preparation in 5.1(i):
V skladu s pripravo testnih objektnih stekelc v 5.1(i): Pripravimo niz dvakratnih razredčin.
Prepare a set of twofold dilutions The first well should have 1/2 of the titre (T/2), the others 1/4 of the titre (T/4), 1/2 of the titre (T/2), the titre (T) and twice the titre (2T).
Prva vdolbinica mora imeti 1/2 titra (T/2), druge pa 1/4 titra (T/4), 1/2 titra (T/2), titer (T) in dvakratni titer (2T).
According to test slide preparation in 5.1(ii):
V skladu s pripravo testnih objektnih stekelc v 5.1(ii):
Prepare the working dilution (WD) of the antibody in IF buffer.
Pripravimo delovno razredčino (DR) protitelesa v pufru IF.
Preparation of the test slide according to 5.1(i) and 5.3(i)
V skladu s pripravo testnih objektnih stekelc v 5.1(i) in 5.3(i)
Arrange the slides on moist tissue paper.
Objektna stekelca razporedimo po vlažnem vpojnem papirju.
Cover each test window completely with the antibody dilution(s).
Testna okenca prekrijemo z razredčino oziroma razredčinami protiteles.
The volume of antibody applied on each window must be at least the volume of extract applied.
Količina protiteles, nanesenih na okenca, mora biti vsaj enaka količini nanesenega ekstrakta.
The following procedure should be carried out in the absence of specific instructions from the suppliers of the antibodies:
Temu postopku sledimo, če ni specifičnih navodil dobaviteljev protiteles.
Incubate the slides on moist paper under a cover for 30 minutes at ambient temperature (18 to 25 °C).
Stekelca inkubiramo pokrita na vlažnem papirju 30 minut pri sobni temperaturi (18-25 °C).
Shake the droplets off each slide and rinse carefully with IF buffer.
S stekelc otresemo kapljice in stekelca previdno speremo s pufrom IF.
Wash by submerging for five minutes in IF buffer-Tween (Appendix 4) and subsequently in IF buffer.
Za pet minut potopimo v pufru IF-Tween (Dodatek 4) in nato še v pufru IF.
Avoid causing aerosols or droplet transfer that could result in cross-contamination.
Izogibamo se rabi pršil ali prenosu kapljic, kar bi lahko privedlo do navzkrižne kontaminacije.
Carefully remove excess moisture by blotting gently.
Previdno popivnamo odvečno vlago.