natgillin/translations-raw huggingface dataset:
The working dilution affects the specificity.
Delovna razredčina vpliva na specifičnost.
Preparation of the test slide according to 4.1(i) and 4.3(i)
Priprava testnega objektnega stekelca v skladu s 4.1(i) in 4.3(i)
Dilutions of resuspended pellet
Razredčine resuspendirane pelete
Dilution of resuspended pellet
Razredčina resuspendirane pelete
Twofold dilutions of antiserum/antibody
Dvakratne razredčine antiseruma/protitelea
Sample 1
Vzorec 1
Duplicate of sample 1 or sample 2
Ponovitev vzorca 1 ali vzorec 2
Preparation of the test slide according to 4.1(ii) and 4.3(ii)
Priprava testnega objektnega stekelca v skladu s 4.1(ii) in 4.3(ii)
Working dilution of antiserum/antibody
Delovna razredčina antiseruma/protitelesa
Decimal dilution of resuspended pellet
Desetkratna razredčina resuspendirane pelete
Arrange the slides on moist paper.
Objektna stekelca razporedimo po vlažnem papirju.
Cover each test window completely with the antibody dilution(s).
Vsako testno okence popolnoma prekrijemo z razredčino ali razredčinami protiteles.
The volume of antibody applied on each window must be at least the volume of extract applied.
Količina protiteles, ki se nanese na vsako okence, mora biti vsaj enaka količini uporabljenega ekstrakta.
The following procedure should be carried out in the absence of specific instructions from the suppliers of the antibodies:
Kadar ni posebnih navodil od dobavitelja protiteles, se izvede naslednji postopek:
Incubate the slides on moist paper under a cover for 30 minutes at ambient temperature (18 to 25 °C).
Inkubiramo objektna stekelca na vlažnem papirju v komori pri sobni temperaturi (18 do 25 °C) 30 minut.
Shake the droplets off each slide and rinse carefully with IF buffer.
Otresemo kapljice z vsakega objektnega stekelca in pazljivo splaknemo s pufrom IF.
Wash by submerging for 5 minutes in IF buffer-Tween (Appendix 3) and subsequently for 5 minutes in IF buffer.
Operemo, tako da potopimo za 5 minut v pufer IF-Tween (Dodatek 3) in nato za 5 minut v pufer IF.
Avoid causing aerosols or droplet transfer that could result in cross-contamination.
Izogibamo se povzročanju pršenja in prenašanju kapljic, ki bi lahko povzročilo navzkrižno okužbo.
Carefully remove excess moisture by blotting gently.
Pazljivo odstranimo odvečno vlago z nežnim pivnanjem.
Cover the test windows with the dilution of FITC conjugate used to determine the titre.
Testna okenca prekrijemo z razredčino konjugata FITC, ki se uporablja za določitev titra.
The volume of conjugate applied on the windows must be identical to the volume of antibody applied.
Količina konjugata, nanesenega na okenca, mora biti enaka količini nanesenega protitelesa.
Shake the droplets of conjugate off the slide.
Otresemo kapljice konjugata z objektnega stekelca.
Rinse and wash as before (4.3.3).
Splaknemo in operemo tako kot prej (4.3.3).
Carefully remove excess moisture.
Pazljivo odstranimo odvečno vlago.
Pipette 5 to 10 µl of 0,1M phosphate-buffered glycerol (Appendix 3) or a commercially antifading mountant on each window and apply a coverslip.
S pipeto kanemo 5 do 10 µl 0,1 M glicerola s fosfatnim pufrom (Dodatek 3) ali komercialno prekrivno sredstvo proti bledenju in pokrijemo s krovnim stekelcem.
Reading the IF test:
Branje testa IF:
Examine test slides on an epifluorescence microscope with filters suitable for excitation of FITC, under oil or water immersion at a magnification of 500 to 1000.
Pregledamo objektna stekelca pod mikroskopom, opremljenim z virom epiflourescentne svetlobe in filtri, ki so primerni za vzbujanje FITC, v imerzijskem olju ali vodi, pri povečavi 500–1000.
Scan windows across two diameters at right angles and around the perimeter.
Okenca pregledamo po črtah dveh pravokotnih premerov in po obodu.
For samples showing no or low number of cells observe at least 40 microscope fields.
Pri vzorcih, ki nimajo ali imajo majhno število celic, pregledamo vsaj 40 mikroskopskih polj.
Check the positive control slide first.
Najprej pregledamo objektno stekelce pozitivne kontrole.
Cells must be bright fluorescent and completely stained at the determined antibody titre or working dilution.
Celice morajo biti svetlo fluorescentne in popolnoma obarvane pri določenem titru protiteles ali delovni razredčini.
The IF test (section 4) must be repeated if the staining is aberrant.
Test IF (oddelek 4) se mora ponoviti v primeru odstopanja pri obarvanju.
Observe for bright fluorescing cells with characteristic morphology of C. m. subsp. sepedonicus in the test windows of the test slides (see web site http://forum.europa.eu.int/Public/irc/sanco/Home/main).
Iščemo svetlo fluorescentne celice z značilno morfologijo C. m. subsp. sepedonicus v testnih okencih testnih objektnih stekelc (glej spletno stran http://forum.europa.eu.int/Public/irc/sanco/Home/main).
The fluorescence intensity must be equivalent or better to the positive control strain at the same antibody dilution.
Intenzivnost fluorescence mora biti enaka ali večja kot pri sevu pozitivne kontrole pri enaki razredčini protitelesa.
Cells with incomplete staining or with weak fluorescence must be disregarded.
Celice, ki niso popolnoma obarvane, ali s šibko fluorescenco se ne upoštevajo.
If any contamination is suspected the test must be repeated.
Če se sumi na okužbo, se test ponovi.
This may be the case when all slides in a batch show positive cells due to the contamination of buffer or if positive cells are found (outside of the slide windows) on the slide coating.
To se lahko zgodi v primeru, ko vsa objektna stekelca v seriji kažejo pozitivne celice zaradi okužbe pufra ali če odkrijemo pozitivne celice (zunaj okenca objektnega stekelca) na zgornji plasti objektnega stekelca.
There are several problems inherent to the specificity of the immunofluorescence test.
S specifičnostjo imunoflourescentnega testa je povezanih nekaj težav.
Background populations of fluorescing cells with atypical morphology and cross reacting saprophytic bacteria with size and morphology similar to C. m. sepedonicus are likely to occur in potato heel end core and stem segment pellets.
V peletah jedra stolona in delov stebel krompirja se bodo verjetno pojavile populacije fluorescentnih celic ozadja z neznačilno morfologijo in navzkrižno reagirajoče saprofitske bakterije, podobne velikosti in morfologije kot C. m. sepedonicus.
Consider only fluorescing cells with typical size and morphology at the titre or working dilution of the antibodies as in 4.3.
Upoštevamo samo fluorescentne celice značilne velikosti in morfologije v titru ali delovni razredčini protiteles kot v 4.3.
Interpretation of the IF reading:
Interpretacija odčitka IF:
If bright fluorescing cells with characteristic morphology are found, estimate the average number of typical cells per microscope field and calculate the number of typical cells per ml of resuspended pellet (Appendix 4).
Če najdemo svetlo fluorescentne celice z značilno morfologijo, ocenimo povprečno število značilnih celic na mikroskopsko polje in izračunamo število značilnih celic na ml resuspendirane pelete (Dodatek 4).
The IF reading is positive for samples with at least 5 × 103 typical cells per ml of resuspended pellet.
Odčitek IF je pozitiven pri vzorcih z vsaj 5 × 103 značilnih celic na ml resuspendirane pelete.
The sample is considered potentially contaminated. and further testing is required.
Vzorec se šteje za potencialno okuženega in potrebno je nadaljnje testiranje.
The IF reading is negative for samples with less than 5 × 103 cells per ml resuspended pellet and the sample is considered negative.
Odčitek IF je negativen pri vzorcih z manj kot 5 × 103 celic na ml resuspendirane pelete in vzorec se šteje za negativnega.
Further testing is not required.
Nadaljnje testiranje ni potrebno.
When the FISH test is used as the first screening test and found to be positive, the IF test must be performed as a second compulsory screening test.
Kadar se uporabi test FISH kot glavni presejalni test in je rezultat pozitiven, se kot obvezni drugi presejalni test opravi test IF.
When the FISH test is used as the second screening test and found to be positive, further testing according to the flow scheme is required to complete the diagnosis.
Kadar se test FISH uporabi kot drugi presejalni test in je rezultat pozitiven, je za končno diagnozo potrebno nadaljnje testiranje v skladu z diagramom poteka.
Use validated C. m. subsp. sepedonicus-specific oligo-probes (Appendix 7).
Uporabimo potrjene oligo-sonde, specifične za C. m. subsp. sepedonicus (Dodatek 7).
Preliminary testing with this method should permit reproducible detection of at least 103 to 104 cells of C. m. subsp. sepedonicus per ml added to sample extracts which previously tested negative.
Predhodno testiranje s to metodo mora omogočati ponovljivo odkrivanje vsaj 103 do 104 celic C. m. subsp. sepedonicus na ml dodano ekstraktom vzorcev, ki so bili prej testirani z negativnim rezultatom.
The following procedure should preferably be performed on freshly prepared sample extract but can also be successfully performed on sample extract that has been stored under glycerol at –16 to –24 °C or –68 to –86 °C.
Naslednji postopek je najbolje izvesti na sveže pripravljenem ekstraktu vzorca, vendar ga prav tako lahko uspešno izvedemo na ekstraktu vzorca, ki je bil predhodno shranjen pri –16 do –24 °C ali –68 do –86 °C pod glicerolom.
As negative controls, use aliquots of sample extract that previously tested negative for C. m. subsp. sepedonicus.
Za negativne kontrole uporabimo alikvote ekstrakta vzorca, ki je bil predhodno testiran z negativnim rezultatom na C. m. subsp. sepedonicus.
As positive controls prepare suspensions containing 105 to 106 cells per ml of C. m. subsp. sepedonicus (e.g. strain NCPPB 4053, or PD 406) in 0,01M phosphate buffer (PB) from a three to five day culture (preparation see Appendix 2).
Za pozitivne kontrole pripravimo suspenzije, ki vsebujejo 105 do 106 celic na ml C. m. subsp. sepedonicus (npr. sev NCPPB 4053 ali PD 406) v 0,01 M fosfatnega pufra (PB) iz 3- do 5-dnevne kulture (za pripravo glej Dodatek 2).
Prepare separate positive control slides of the homologous strain or any other reference strain of C. m. subsp. sepedonicus, suspended in potato extract, as specified in Appendix 2.
Pripravimo ločena objektna stekelca pozitivne kontrole homolognega seva ali katerega drugega referenčnega seva C. m. subsp. sepedonicus, suspendiranega v ekstraktu krompirja, kot je določeno v Dodatku 2.
The use of the FITC-labelled eubacterial oligo-probe offers a control for the hybridisation process, since it will stain all eubacteria that are present in the sample.
Uporaba evbakterijske oligo-sonde z oznako FITC nudi kontrolo za proces hibridizacije, saj obarva vse evbakterije, ki se nahajajo v vzorcu.
Potato extract fixation
Fiksacija ekstrakta krompirja
Prepare fixative solution (see Appendix 7).
Pripravimo fiksirno raztopino (glej Dodatek 7).
Pipette 100 µl of each sample extract into an Eppendorf tube and centrifuge for eight min at 7000 g.
S pipeto kanemo 100 µl vsakega ekstrakta vzorca v Eppendorfovo epruveto in centrifugiramo 8 min na 7000 g.
Remove the supernatant and dissolve the pellet in 500 µl of fixative prepared < 24 hours previously.
Odlijemo supernatant in raztopimo peleto v 500 µl preparata za fiksiranje, pripravljenega manj kot 24 ur pred tem.
Vortex and incubate overnight at 4 °C.
Premešamo z vrtinčenjem in inkubiramo čez noč pri 4 °C.
An alternative fixative is 96 % ethanol.
Alternativni preparat za fiksiranje je 96 % etanol.
To use this dissolve the pellet from step 5.1.2 in 50 µl 0,01M PB and 50 µl 96 % ethanol.
Pri tem je treba raztopiti peleto iz stopnje 5.1.2 v 50 µl 0,01 M PB in 50 µl 96 % etanola.
Vortex mix and incubate at 4 °C for 30 to 60 minutes.
Premešamo z vrtinčenjem in inkubiramo 30 do 60 minut pri 4 °C.
Centrifuge for 8 min. at 7000 g, remove the supernatant and resuspend the pellet in 75 µl 0,01M PB (see Appendix 3).
Centrifugiramo 8 minut pri 7000 g, odlijemo supernatant in resuspendiramo peleto v 75 µl 0,01 M PB (glej Dodatek 3).
The remaining sample solution (49 µl) can be stored at –20 °C after addition of 1 volume of 96 % ethanol.
Preostala raztopina vzorca (49 µl) se lahko shrani pri –20 °C, potem ko dodamo eno količino 96 % etanola.
In case the FISH assay requires repeating, remove the ethanol by centrifugation and add an equal volume of 0,01M PB (mix by vortexing).
V primeru, ko je treba preskušanje FISH ponoviti, odstranimo etanol s centrifugiranjem in dodamo enako količino 0,01 M PB (premešamo z vrtinčenjem).
Sample 2
Vzorec 2
window 1
okence 1
window 2
okence 2
window 3
okence 3
window 4
okence 4
window 5
okence 5
window 6
okence 6
window 7
okence 7
window 8
okence 8
window 9
okence 9
window 10
okence 10
Coverslip 1
Krovno stekelce 1
Coverslip 2
Krovno stekelce 2
Air-dry the slides (or on slide dryer at 37 °C) and fix them by flaming.
Objektna stekelca posušimo na zraku (ali v sušilniku za stekelca pri 37 °C) in jih fiksiramo nad plamenom.
At this stage the procedure may be interrupted and the hybridisation continued the following day.
Na tej stopnji se postopek lahko prekine in nadaljuje s hibridizacijo naslednji dan.
Slides should be stored dust-free and dry at room temperature.
Objektna stekelca se shranijo v prostoru brez prahu in na suhem pri sobni temperaturi.
Pre-hybridisation and hybridisation
Predhibridizacija in hibridizacija
Prepare a lysozyme solution containing 10 mg lysozyme (Sigma L–6876) in 10 ml buffer (100 mM Tris-HCl, 50 mM EDTA, pH8.0).
Pripravimo raztopino lizocima, ki vsebuje 10 mg lizocima (Sigma L-6876) v 10 ml pufra (100 mM Tris-HCl, 50 mM EDTA, pH 8,0).
This solution can be stored but it should only be freeze-thawed once.
Ta raztopina se lahko shrani, vendar jo lahko samo enkrat zamrznemo in odtalimo.
Cover each sample well with approximately 50 µl of lysozyme solution and incubate for 10 minutes at room temperature.
Vsak vzorec dobro prekrijemo s približno 50 µl raztopine lizocima in 10 minut inkubiramo pri sobni temperaturi.
Then dip the slides in demineralised water, once only and dry with filter paper.
Nato samo enkrat pomočimo objektna stekelca v demineralizirano vodo in osušimo s filtrirnim papirjem.
Alternatively, instead of lysozyme add 50 µl of 40 to 400µg ml–1 proteinase K in buffer (20 mM Tris-HCl, 2 mM CaCl2, pH 7,4) to each well and incubate at 37 °C for 30 minutes.
Alternativno namesto lizocima dodamo 50 µl 40-400 ug ml–1 proteinaze K v pufru (20 mM Tris-HCl, 2 mM CaCl2, pH 7,4) na vsako vdolbinico in inkubiramo 30 minut pri 37 °C.
Dehydrate the cells in a graded ethanol series of 50 %, 80 % and 96 % for one minute each.
Dehidriramo celice v stopenjski seriji 50 %, 80 % in 96 % etanola, v vsakem po eno minuto.
Air dry the slides in a slide-holder.
Objektna stekelca posušimo v stojalu za stekelca.
Prepare a moist incubation chamber by covering the bottom of an air-tight box with tissue or filter paper soaked in 1x hybmix (Appendix 7).
Pripravimo vlažno inkubacijsko komoro, tako da pokrijemo dno hermetične posode z pivnim ali filtrirnim papirjem, prepojenim z 1x hybmix. (Dodatek 7).
Pre-incubate the box in the hybridisation oven at 55 °C for at least 10 minutes.
Predinkubiramo posodo v hibridizacijski pečici vsaj 10 minut pri 55 °C.
Prepare the hybridisation solution (Appendix 7) allowing 45 µl per slide, and preincubate for five minutes at 55 °C.
Pripravimo hibridizacijsko raztopino (Dodatek 7), pri čemer nanesemo 45 µl na objektno stekelce in predinkubiramo 5 minut pri 55 °C.
Place slides on a hot plate at 45 °C and apply 10 µl of hybridization solution to each of the four wells on the slide(s).
Položimo objektna stekelce na vročo ploščo pri 45 °C in nanesemo 10 µl hibradizacijske raztopine na vse štiri vdolbinice na objektnem stekelcu ali stekelcih.
Apply two coverslips (24 × 24 mm) to each slide without trapping air.
Nanesemo 2 krovni stekelci (24 × 24 mm) na vsako objektno stekelce in pazimo, da se ne napravijo zračni mehurčki.
Place the slides in the pre-warmed moist chamber and hybridise overnight in the oven at 55 °C in the dark.
Objektna stekelca položimo v prej ogreto vlažno komoro in čez noč v temi hibridiziramo v pečici pri 55 °C.
Prepare three beakers containing 1 l of Ultra pure water (UPW), 1 l of 1x hybmix (334 ml 3x hybmix and 666 ml UPW) and 1 l of 1/2x hybmix (167 ml 3x hybmix and 833 ml UPW).
Pripravimo 3 čaše, ki vsebujejo 1 l ultra čiste vode (UPW), 1 l 1x hybmixa (334 ml 3x hybmixa in 666 ml UPW) in 1 l 1/2x hybmixa (167 ml 3x hybmixa in 833 ml UPW).
Pre-incubate each in a waterbath at 55 °C.
Vsako predinkubiramo v vodni kopeli pri 55 °C.
Remove the coverslips from the slides and place the slides in a slide holder.
Odstranimo krovna stekelca z objektnih stekelc in namestimo objektna stekelca v stojalo za stekelca.
Wash away excess probe by incubation for 15 mins. in the beaker with 1x hybmix at 55 °C.
Izperemo odvečno sondo z inkubiranjem v čaši z 1x hybmixom pri 55 °C 15 minut.
Transfer the slide holder to 1/2 hybmix washing solution and incubate for a further 15 mins.
Prenesemo stojalo za stekelca v pralno raztopino 1/2 hybmix in inkubiramo nadaljnjih 15 minut.
Dip the slides briefly in UPW and place them on filter paper.
Objektna stekelca na hitro pomočimo v UPW in jih položimo na filtrirni papir.
Remove excess moisture by covering the surface gently with filter paper.
Odstranimo odvečno vlago, tako da površino nalahno prekrijemo s filtrirnim papirjem.
Pipette 5 to 10 μl of anti-fading mountant solution (e.g. Vectashield, Vecta Laboratories, CA, USA or equivalent) on each window and apply a large coverslip (24 × 60 mm) over the whole slide.
S pipeto kanemo 5 do 10 μl prekrivne raztopine proti bledenju (npr. Vectashield, Vecta Laboratories, CA, USA ali enakovredni) na vsako okence in položimo široko krovno stekelce (24 × 60 mm) čez celo objektno stekelce.
Reading the FISH test
Branje testa FISH
Observe the slides immediately with a microscope fitted for epifluorescence microscopy at 630 or 1000x magnification under immersions oil.
Nemudoma pregledamo objektna stekelca pod mikroskopom, opremljenim z virom epiflourescentne svetlobe, pri povečavi 630 ali 1000x v imerzijskem olju.
With a filter suitable for fluorescein isothiocyanate (FITC) eubacterial cells (including most gram negative cells) in the sample are stained fluorescent green.
S filtrom, primernim za fluorescein izotiocinat (FITC), se evbakterijske celice (vključno z večino gram negativnih celic) v vzorcu obarvajo fluorescentno zeleno.
Using a filter for tetramethylrhodamine-5-isothiocyanate, Cy3-stained cells of C. m. subsp. sepedonicus appear fluorescent red.
Z uporabo filtra za tetrametilrodamin-5-izotiocinat, izgledajo celice C. m. subsp. sepedonicus, obarvane s Cy3, fluorescentno rdeče. Primerjamo morfologijo celic s tistimi iz pozitivnih kontrol.
Compare cell morphology with that of the positive controls.
Celice morajo biti svetlo fluorescentne in popolnoma obarvane.
Cells must be bright fluorescent and completely stained The FISH test (section 9.4) must be repeated if the staining is aberrant.
V primeru odstopanja pri obarvanju se mora ponoviti test FISH (oddelek 9.4).
The fluorescence intensity must be equivalent or better than that of the positive control strain.
Intenzivnost fluorescence mora biti enaka ali večja kot pri sevu pozitivne kontrole.
There are several problems inherent to the specificity of the FISH test.
S specifičnostjo testa FISH je povezanih nekaj težav.
Background populations of fluorescing cells with atypical morphology and cross reacting saprophytic bacteria with size and morphology similar to C. m. subsp. sepedonicus may occur, although much less frequent than in the IF test, in potato heel end core and stem segment pellets.
V peletah jedra stolona in delov stebel krompirja se lahko pojavijo populacije fluorescentnih celic ozadja z neznačilno morfologijo in navzkrižno reagirajoče saprofitske bakterije, podobne velikosti in morfologije kot C. m. sepedonicus, čeprav veliko manj pogosto kot pri testu IF.
Consider only fluorescing cells with typical size and morphology, see in 5.3.2.
Upoštevamo samo fluorescentne celice značilne velikosti in morfologije, glej 5.3.2.
Interpretation of the FISH test result:
Interpretacija rezultatov testa FISH:
Valid FISH test results are obtained if bright green fluorescent cells of size and morphology typical of C. m. subsp. sepedonicus are observed using the FITC filter and if bright red fluorescent cells using the rhodamine filter in all positive controls and not in any of the negative controls.
Veljavne rezultate testa FISH dobimo, če z uporabo filtra FITC opazimo svetlo zeleno fluorescentne celice, velikosti in morfologije značilne za C. m. subsp. sepedonicus, in svetlo rdeče fluorescentne celice z uporabo filtra rodamin, pri vseh pozitivnih kontrolah in pri nobeni od negativnih kontrol.
Samples with at least 5 × 103 typical cells per ml of resuspended pellet are considered potentially contaminated.
Vzorci z vsaj 5 × 103 značilnih celic na ml resuspendirane pelete se štejejo za potencialno okužene.
Further testing is required.
Potrebno je nadaljnje testiranje.
Samples with less than 5 × 103 typical cells per ml of resuspended pellet are considered negative.
Vzorci z manj kot 5 × 103 značilnih celic na ml resuspendirane pelete se štejejo za negativne.
The FISH test is negative if bright red fluorescent cells with size and morphology typical of C. m. subsp. sepedonicus are not observed using the rhodamine filter, provided that typical bright red fluorescent cells are observed in the positive control preparations when using the rhodamine filter.
Test FISH je negativen, če z uporabo filtra rodamin ne opazimo svetlo rdeče fluorescentnih celic, velikosti in morfologije značilne za C. m. subsp. sepedonicus, če z uporabo filtra rodamin opazimo značilne svetlo rdeče fluorescentne celice pri preparatih pozitivnih kontrol.
When the PCR test is used as the principal screening test and found to be positive, the IF test must be performed as a second compulsory screening test.
Kadar se uporabi test PCR kot glavni presejalni test in je rezultat pozitiven, se kot obvezni drugi presejalni test opravi test IF.
When the PCR test is used as the second screening test and found to be positive, further testing according to the flow scheme is required to complete the diagnosis.
Kadar se test PCR uporabi kot drugi presejalni test in je rezultat pozitiven, je za končno diagnozo potrebno nadaljnje testiranje v skladu z diagramom poteka.
Full exploitation of this method as principal screening test is only recommended when specialised expertise has been acquired.
Popolna uporaba te metode kot glavnega presejalnega testa je priporočena samo ob pridobitvi specialističnega strokovnega znanja.
Preliminary testing with this method should permit reproducible detection of 103 to 104 cells of C. m. subsp. sepedonicus per ml added to sample extracts which previously tested negative.
Predhodno testiranje s to metodo mora omogočati ponovljivo odkrivanje vsaj 103 do 104 celic C. m. subsp. sepedonicus na ml dodano ekstraktom vzorcev, ki so bili prej testirani z negativnim rezultatom.
Optimisation experiments may be required to achieve maximum levels of sensitivity and specificity in all laboratories.
Lahko so potrebni optimizacijski poskusi za dosego maksimalnih stopenj občutljivosti in specifičnosti v vseh laboratorijih.
Use validated PCR reagents and protocols.
Uporabimo potrjene reagente in protokole PCR.
Preferably select a method with an internal control.
Raje izberemo metodo z notranjo kontrolo.
Use appropriate precautions to avoid contamination of sample with target DNA.
Uporabimo ustrezno previdnostne ukrepe, da se ognemo okužbi vzorca s ciljno DNK.
The PCR test should be performed by experienced technicians, in dedicated molecular biology laboratories, in order to minimise the possibility of contamination with target DNA.
Test PCR morajo opravljati izkušeni tehniki v namenskih laboratorijih za molekularno biologijo, da bi čim bolj zmanjšali možnost okužbe s ciljno DNK.
Negative controls (for DNA extraction and PCR procedures) should always be handled as final samples in the procedure, to make evident whether any carry over of DNA has occurred.
Negativne kontrole (za ekstrakcijo DNK in postopke PCR) morajo biti vedno obravnavane kot končni vzorci v postopku, da je očitno, ali se je zgodil kak prenos DNK.