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A range-finding study should last a minimum of 5 days and generally no more than 28 days. The rationale for the selection of concentrations for the main study should be provided in the study report. The objective of the main study is to demonstrate a concentration-response relationship based on what is anticipated to be the most sensitive endpoint.
Un studiu de stabilire a intervalului durează cel puțin 5 zile și, în general, nu mai mult de 28 de zile.
If the LOEC/NOEC are to be estimated, five test concentrations with at least four replicates should be used and the factor between concentrations should not be greater than two. The number of replicates should be sufficient to ensure adequate statistical power to detect a 20 % difference from the control at the 5 % level of significance (p = 0,05).
Atunci când urmează să se estimeze CMEO sau CFEO, trebuie să se utilizeze cinci concentrații de testare cu cel puțin patru probe duplicat, iar factorul dintre concentrații nu trebuie să fie mai mare de doi.
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Ensure that sufficient oxygen is always available (> 2 mg/l) and that the temperature is kept in the required range (normally 20 °C to 25 °C). Keep the pH at 7,5 ± 0,5 by dosing small amounts of inorganic base or acid into the aeration vessel or into the influent, or by increasing the buffering capacity of the organic medium (see paragraph 27).
Se asigură că există întotdeauna suficient oxigen (> 2 mg/l) și că temperatura este menținută în intervalul necesar (în mod normal, între 20 și 25 °C). pH-ul se menține la 7,5 ± 0,5 prin dozarea unor cantități mici de bază sau acid anorganic în vasul de aerare sau prin creșterea capacității de tamponare a mediului organic (a se vedea punctul 27).
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Avertizare: Nămolul activ, apa uzată și efluentul de apă uzată conțin organisme patogene și trebuie manipulate cu precauție.
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În cadrul unui studiu tradițional, grupurile de animale sunt expuse la o substanță chimică de testare pentru o perioadă fixă (în general, 4 ore) într-o incintă de expunere în zona nasului sau a întregului corp. Animalele sunt expuse la o concentrație-limită (test la valori-limită) sau la minimum trei concentrații într-o procedură secvențială (studiu principal).
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The organs and tissues that have lesions in the high concentration group should be examined in all groups. The study director may choose to perform histopathological evaluations for additional groups to demonstrate a clear concentration response.
Organele și țesuturile care prezintă leziuni în grupul de concentrație ridicată se examinează la toate grupurile Conducătorul studiului poate alege să efectueze evaluările histopatologice pentru grupuri suplimentare, pentru a demonstra o relație clară concentrație-răspuns.
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