natgillin/translations-raw huggingface dataset:
Note: The column shall be cleaned by flushing with 100 % solvent B (7.5.1) for at least 30 minutes every 20-25 runs.
Otapala za eluaciju (4.4.)
Integration mode
Otapalo A: Otopina 0,3 mM natrijeva dihidrogen fosfata i 3 mM otopine natrijeva acetata (pH vrijednosti podešene octenom kiselinom na 6,5 ± 0,1): metanol: tetrahidrofuran = 558:440:2 (v/v/v).
PEDP peak
Otapalo B: metanol
PEDP is eluted as a single peak.
Preporučeni gradijent eluacije:
Determine the peak area by valley-to- valley integration.
Vrijeme (min)
Tryptamine peak
Otapalo A (%):
Tryptamine is eluted as a single peak (Figure 1).
Otapalo B (%):
Determine the peak area by valley-to-valley integration.
Brzina protoka (ml/min)
PS and PE peaks groups
Početak
Under the described conditions (Figure 1), PS elutes as two main partially unresolved peaks preceded by a minor peak.
0Napomena: Da bi se postigla rezolucija prikazana na slici 1., gradijent eluacije možda treba malo modificirati.
PE elutes as three main partially unresolved peaks.
Temperatura kolone: 30 °C.
Determine the whole area of each peak cluster setting the baseline as reported in Figure 1.
Volumen injektiranja: 50 μl reagensa za derivatizaciju i 50 μl otopine uzorka.
CALCULATION AND EXPRESSION OF RESULTS
Uspostavljanje ravnoteže u koloni
PS and PE content in the test sample shall be calculated as follows:
Svakog dana pri pokretanju sustava kolonu treba ispirati 15 minuta 100 %-tnim otapalom B, a potom podesiti na A:B = 40:60 da bi se tijekom 15 minuta uspostavila ravnoteža uz brzinu protoka od 1 ml/min.
where:
Napravite slijepu probu ubrizgavanjem metanola (4.2.1.).
C = PS or PE content (mg/100 g powder) in the test sample
Napomena: Prije nego kolonu pohranite na duže vrijeme, ispirite je 30 minuta mješavinom metanol: kloroform = 80:20 (v/v).
A 1 = PEDP peak area of the standard sample solution (7.3)
Odredite sadržaj fosfatidilserina (PS) i fosfatidiletanolamina (PE) u ispitnom uzorku.
A 2 = PS or PE peak area of the test sample solution (7.2)
Izvedite niz kromatografskih analiza pazeći da vrijeme između ciklusa bude konstantno radi postizanja konstantnih vremena zadržavanja.
T 1 = Tryptamine peak area of the standard sample solution (7.3)
Nakon svakih 5-10 otopina ispitnog uzorka ubrizgajte vanjsku standardnu otopinu (7.3.) radi izračuna faktora odziva.
T 2 = Tryptamine peak area of the test sample solution (7.2).
Napomena: Nakon svakih 20-25 analiza kolona se mora ispirati 100 %-tnim otapalom B (7.5.1.) najmanje 30 minuta.
ACCURACY OF THE METHOD
Integracija
Note: The values for repeatability were calculated according to the IDF International Standard (*).
Pik fosfatidiletanolamin dipalmitoila (PEDP)
Repeatability
PEDP se pojavljuje kao jedan pik.
If two determinations are obtained under these conditions, the relative difference between the two results should not be greater than 6 % of the arithmetic mean of the results.
Pik triptamina
Reproducibility
Triptamin se pojavljuje kao jedan pik (slika 1.).
If two determinations are obtained by operators in different laboratories using different apparatus under different conditions for the analysis on the same test sample, the relative difference between the two results should not be greater than 11 % of the arithmetic mean of the results.
Odredite površinu pika integracijom dolina-dolina.
REFERENCES
Grupe pikova fosfatidilserina (PS) i fosfatidiletanolamina (PE)
Resmini P., Pellegrino L., Hogenboom J.A., Sadini V., Rampilli M., ‘Detection of buttermilk solids in skimmilk powder by HPLC quantification of aminophospholipids’.
U opisanim se uvjetima (slika 1.)
Figure 1
PS pojavljuje kao dva glavna djelomično nerazdvojena pika, kojima prethodi jedan manji pik.
HPLC pattern of OPA-derivatives of phosphatidylserine (PS) and phosphatidylethanolamine (PE) in methanol extract of reconstituted skim-milk powder.
PE sa javlja kao tri glavna djelomično nerazdvojena pika.
Integration mode for the peaks of PS, PE and tryptamine (internal standard) is reported
Odredite ukupnu površinu svake grupe pikova postavljajući baznu liniju kako je prikazano na slici 1.
Fluorescence (arbitrary units)
IZRAČUN I PRIKAZ REZULTATA
Appendix II
Sadržaj fosfatidilserina (PS) i fosfatidiletanolamina (PE) u ispitnom uzorku izračunava se s pomoću sljedeće formule:
DETECTION OF RENNET WHEY IN SKIMMED MILK POWDER FOR PUBLIC STORAGE BY DETERMINATION OF CASEINOMACROPEPTIDES HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
gdje je:
SCOPE AND FIELD OF APPLICATION
C = sadržaj fosfatidilserina (PS) ili fosfatidiletanolamina (PE) (mg/100 g praha) u ispitnom uzorku;
This method allows detection of rennet whey in skimmed milk powder intended for public storage by determination of the caseinomacropeptides.
A 1 = površina pika fosfatidiletanolamin dipalmitoila (PEDP) u standardnoj otopini uzorka (7.3.);
REFERENCE
A 2 = površina pika fosfatidilserina (PS) ili fosfatidiletanolamina (PE) u ispitnoj otopini uzorka (7.2.);
International Standard ISO 707 - Milk and Milk Products - Guidance on sampling.
T 1 = površina pika triptamina u standardnoj otopini uzorka (7.3.);
The content of rennet whey solids is defined as the percentage by mass as determined by the caseinomacropeptide content by the procedure described.
T 2 = površina pika triptamina u ispitnoj otopini uzorka (7.2.).
PRINCIPLE
PRECIZNOST METODE
Reconstitution of the skimmed milk powder, removal of fat and proteins with trichloroacetic acid, followed by centrifugation or filtration;
Napomena: Vrijednosti ponovljivosti računaju se u skladu s međunarodnom normom IDF (*).
Determination of the quantity of caseinomacropeptides (CMP) in the supernatant by high-performance liquid chromatography (HPLC);
Ponovljivost
Evaluation of the result obtained for the samples by reference to standard samples consisting of skimmed milk powder with or without the addition of a known percentage of whey powder.
Relativna standardna devijacija ponovljivosti, koja prikazuje varijabilnost nezavisnih analitičkih rezultata koje dobije isti analitičar koristeći se istom opremom u istim uvjetima i na istom ispitnom uzorku u kratkom vremenskom intervalu, ne smije biti veća od 2 % relativno.
The water used shall be distilled water or water of at least equivalent purity.
Ako se dva određivanja izvrše pod navedenim uvjetima, relativna razlika između dvaju rezultata ne bi smjela biti veća od 6 % aritmetičke sredine rezultata.
Trichloroacetic acid solution
Obnovljivost
Dissolve 240 g of trichloroacetic acid (CCl3COOH) in water and make up to 1000 ml.
Ako su dva određivanja izvršili analitičari u različitim laboratorijima, u različitim uvjetima, koristeći se različitom opremom, na istom ispitnom uzorku, relativna razlika između dvaju rezultata ne smije biti veća od 11 % aritmetičke sredine rezultata.
The solution should be clear and colourless.
REFERENTNA LITERATURA
Eluent solution, pH 6,0
Slika 1.
Dissolve 1,74 g of dipotassium hydrogen phosphate (K2HPO4), 12,37 g of potassium dihydrogen phosphate (KH2PO4) and 21,41 g of sodium sulphate (Na2SO4) in about 700 ml of water.
HPLC struktura OPA-derivata fosfatidilserina (PS) i fosfatidiletanolamina (PE) u metanolnom ekstraktu rekonstituiranog obranog mlijeka u prahu.
Adjust, if necessary, to pH 6,0, using a solution of phosphoric acid or potassium hydroxide.
Prikazan je način integracije pikova fosfatidilserina (PS), fosfatidiletanolamina (PE) i triptamina (interni standard).
Make up to 1000 ml with water and homogenise.
Fluorescencija (proizvoljno odabrane jedinice)
Note: The composition of the eluent can be updated to comply with the certificate of the standards or the recommendations of the manufacturer of the column packing material.
Dodatak II.
Filter the eluent solution, prior to use, through a membrane filter with a 0,45 μm pore diameter.
DETEKCIJA SLATKE SIRUTKE U OBRANOM MLIJEKU U PRAHU ZA JAVNO SKLADIŠTENJE ODREĐIVANJEM SADRŽAJA KAZEINO-MAKROPEPTIDA METODOM TEKUĆINSKE KROMATOGRAFIJE VISOKE DJELOTVORNOSTI (HPLC)
Flushing solvent
OPSEG I PODRUČJE PRIMJENE
Mix one volume acetonitrile (CH3CN) with nine volumes water.
Ova metoda omogućuje detekciju slatke sirutke u obranom mlijeku u prahu za javno skladištenje određivanjem sadržaja kazeino-makropeptida.
Filter the mixture prior to use through a membrane filter with a 0,45 μm pore diameter.
REFERENCA
Note: Any other flushing solvent with a bactericidal effect which does not impair the columns' resolution efficiency may be used.
Međunarodna norma ISO 707 – Mlijeko i mliječni proizvodi – Smjernice za uzorkovanje.
Standard samples
Sadržaj suhe tvari u slatkoj sirutki definira se kao maseni postotak kazeino-makropeptida prema opisanom postupku.
Skimmed milk powder meeting the requirements of this Regulation (i.e. [0])
NAČELO
The same skimmed milk powder adulterated with 5 % (m/m) rennet-type whey powder of standard composition (i.e. [5])
Rekonstitucija obranog mlijeka u prahu, uklanjanje masti i bjelančevina pomoću triklorooctene kiseline, iza čega slijedi centrifugiranje ili filtracija.
Analytical balance
Utvrđivanje količine kazeino-makropeptida (CMP) u supernatantu metodom tekućinske kromatografije visoke učinkovitosti (HPLC).
Optional centrifuge capable of attaining a centrifugal force of 2200 g, fitted with stoppered or capped centrifuge tubes of about 50 ml capacity
Ocjenjivanje rezultata dobivenih za uzorke na temelju usporedbe sa standardnim uzorcima koji se sastoje od obranog mlijeka u prahu s dodatkom poznatog postotka sirutke u prahu ili bez njega.
Mechanical shaker
Mora se upotrebljavati destilirana voda ili voda barem jednake čistoće.
Glass funnels, diameter about 7 cm
Otopina triklorooctene kiseline
Filter papers, medium filtration, diameter about 12,5 cm
Otopite 240 g triklorooctene kiseline (CCl3CCOOH) u vodi i dopunite do 1000 ml.
Glass filtration equipment with 0,45 μm pore diameter membrane filter
Otopina bi trebala biti bistra i bezbojna.
Graduated pipettes allowing delivery of 10 ml (ISO 648, Class A, or ISO/R 835) or a dispensing system capable of delivering 10,0 ml in two minutes
Otopina eluensa, pH 6,0
Dispensing system capable of delivering 20,0 ml water at ca.
Otopite 1,74 g dikalijeva hidrogen fosfata (K2HPO4), 12,37 g kalijeva dihidrogen fosfata, (KH2PO4) i 21,41 g natrijeva sulfata (Na2SO4) u oko 700 ml vode.
50 °C
Prema potrebi podesiti pH na 6,0 pomoću otopine fosforne kiseline ili kalijeva hidroksida.
Thermostatic water bath, set at 25 ± 0,5 °C
Dopunite vodom do 1000 ml i homogenizirajte.
HPLC equipment, consisting of:
Napomena: Sastav eluensa može se prilagoditi tako da bude u skladu s potvrdom o sukladnosti sa standardima ili preporukama proizvođača materijala za punjenje kolona.
Pump
Otopinu eulensa prije uporabe profiltrirajte kroz membranski filtar s promjerom pora od 0,45 μm.
Injector, hand or automatic, with a 15 to 30 μl capacity
Otopina za ispiranje
Two TSK 2000-SW columns in series (length 30 cm, internal diameter 0,75 cm) or equivalent columns (e.g. single TSK 2000-SWxl, single Agilent Technologies Zorbax GF 250) and a precolumn (3 cm × 0,3 cm) packed with I 125 or material of equivalent effectiveness
Pomiješajte jedan volumen acetonitrila (CH3CN) i devet volumena vode.
Thermostatic column oven, set at 35 ± 1 °C
Prije uporabe profiltrirajte mješavinu kroz membranski filtar s porama promjera 0,45 μm.
Variable wavelength UV detector, permitting measurements at 205 nm with a sensitivity of 0,008 Å
Napomena: Može se upotrebljavati bilo koja druga baktericidna otopina za ispiranje koja ne utječe na djelotvornost kolone u smislu rezolucije.
Integrator capable of valley-to-valley integration
Standardni uzorci
Note: Working with columns kept at room temperature is possible, but their power of resolution is slightly lower.
Obrano mlijeko u prahu koje ispunjava zahtjeve ove Uredbe (tj.
In that case, the temperature should vary by less than ± 5 °C in any one range of analyses.
[0]).
Samples shall be taken in accordance with the procedure laid down in International Standard ISO 707.
Isto mlijeko u prahu s dodatkom 5 % (m/m) slatke sirutke u prahu standardnog sastava (tj.
However, Member States may use another method of sampling provided that it complies with the principles of the abovementioned standard
[5]).
Store the sample in conditions which preclude any deterioration or change in composition
Analitička vaga
Preparation of the test sample
Centrifuga, neobvezna, koja može postići centrifugalnu silu od 2200 g, opremljena epruvetama za centrifugiranje s čepom ili navojnim čepom, zapremine oko 50 ml
Transfer the milk powder into a container with a capacity of about twice the volume of the powder, fitted with an airtight lid.
Mehanička tresilica
Close the container immediately.
Stakleni lijevci promjera oko 7 cm
Mix the milk powder well by means of repeated inversion of the container.
Filtar-papiri za srednju brzinu filtracije, promjera oko 12,5 cm
Test portion
Staklena oprema za filtriranje s membranskim filtrom s promjerom pora 0,45 μm
Weight 2,000 ± 0,001 g of test sample into a centrifuge tube (6.2) or a suitable stoppered flask (50 ml).
Graduirane pipete od 10 ml (ISO 648, Klasa A ili ISO/R 835) ili dozirni sustav kapaciteta dobave 10,0 ml u dvije minute
Removal of fat and proteins
Dozirni sustav koji omogućuje unos 20,0 ml vode na oko 50 °C
Add 20,0 ml of warm water (50 °C) to the test portion.
Vodena kupelj s termostatom, zagrijana na 25 ± 0,5 °C
Dissolve the powder by shaking for five minutes using a mechanical shaker (6.3).
Oprema za HPLC koja se sastoji od sljedećeg:
Place the tube into the water bath (6.10) and allow to equilibrate to 25 °C
Pumpa
Add 10,0 ml of the trichloroacetic acid solution (5.1) of ca.
Injektor, ručni ili automatski, zapremine 15 do 30 μl
25 °C in two minutes, while stirring vigorously with the aid of the magnetic stirrer (6.4).
Dvije kolone u nizu (duljine 30 cm, unutarnjeg promjera 0,75 cm) ili ekvivalentne kolone (npr. jednostruka TSK 2000-SWxl, jednostruka Agilent Technologies Zorbax GF 250) i pretkolona (3 cm × 0,3 cm) napunjena s I 125 ili materijalom jednake djelotvornosti
Place the tube in a water bath (6.10) and leave for 60 minutes
Grijač kolone s termostatom podešen na 35 ± 1 °C
Centrifuge (6.2) for 10 minutes at 2200 g, or filter through paper (6.6), discarding the first 5 ml of filtrate
UV detektor prilagodljive valne duljine koji omogućuje mjerenja na 205 nm uz osjetljivost od 0,008 Å
Chromatographic determination
Integrator s mogućnošću integriranja dolina-dolina.
Inject 15 to 30 μl of accurately measured supernatant or filtrate (8.3.3) into the HPLC apparatus (6.11) operating at a flow rate of 1,0 ml of eluent solution (5.2) per minute
Napomena: Rad s kolonama na sobnoj temperaturi moguć je iako je u tim uvjetima njihova rezolucija niža.
Note 1.
U tom slučaju temperatura ne smije varirati za više od ± 5 °C u bilo kojem rasponu analiza.
Another flow rate may be used, dependent of the internal diameter of the columns used or the instructions of the manufacturer of the column.
Uzorci se uzimaju u skladu s postupkom utvrđenim u međunarodnoj normi ISO 707.
Note 2.
Međutim, države članice mogu primijeniti neku drugu metodu uzorkovanja pod uvjetom da je ona u skladu s načelima prethodno naveden norme.
Rinse the columns with water during each interruption.
Uzorke čuvajte u uvjetima koji onemogućavaju kvarenje ili promjene u njihovu sastavu.
Never leave the eluent solution in them (5.2).
Priprema ispitnog uzorka
Prior to any interruption of more than 24 hours, rinse the columns with water then wash them with solution (5.3) for at least three hours at a flow rate of 0,2 ml per minute.
Stavite mlijeko u prahu u posudu dvostruko većeg volumena od volumena praha, koja ima hermetički poklopac.
The results of chromatographic analysis of the test sample [E] are obtained in the form of chromatogram in which each peak is identified by its retention time RT as follows:
Posudu odmah zatvorite.
Peak II:
Mlijeko u prahu dobro promiješajte uzastopnim preokretanjem posude.
The second peak of the chromatogram having an RT of about 12,5 minutes.
Uzorak za analizu
Peak III:
Odvagnite 2,000 + 0,001 g ispitnog uzorka u epruvetu za centrifugiranje (6.2.) ili u odgovarajuću tikvicu s čepom (50 ml).
The third peak of the chromatogram, corresponding to the CMP, having an RT of 15,5 minutes.
Odstranjivanje masti i bjelančevina
The choice of the column(s) can affect the retention times of the individual peaks considerably.
Uzorku za analizu dodajte 20,0 ml zagrijane vode (50 °C).
The integrator (6.11.6) automatically calculates the area A of each peak:
Otopite prah protresajući ga pet minuta pomoću mehaničke tresilice (6.3.).
area of peak II,
Epruvetu stavite u vodenu kupelj (6.10.) i pustite da se temperatura uravnoteži na 25 °C.
area of peak III,
U dvije minute dodajte 10,0 ml otopine triklorooctene kiseline zagrijane na oko 25 °C (5.1.) uz snažno miješanje magnetskom miješalicom (6.4.).
It is essential to examine the appearance of each chromatogram prior to quantitative interpretation, in order to detect any abnormalities due either to malfunctioning of the apparatus or the columns, or to the origin and nature of the sample analysed.
Epruvetu stavite u vodenu kupelj (6.10.) i ostavite 60 minuta.
If in doubt, repeat the analysis.
Centrifugirajte (6.2.) 10 minuta na 2200 g ili filtrirajte kroz filtar-papir (6.6.), pri čemu prvih 5 ml filtrata bacite.
Calibration
Kromatografsko određivanje
Apply exactly the procedure described from point 8.2 to point 8.4.2 to the standard samples (5.4)
Ubrizgajte 15 do 30 μl točno izmjerenog supernatanta ili filtrata (8.3.3.) u uređaj za HPLC (6.11.), koji radi uz brzinu protoka od 1,0 ml otopine eluensa (5.2.) u minuti.
Use freshly prepared solutions, because CMP degrade in an 8 % trichloroacetic environment.
Napomena 1.: Mogu se koristiti različite brzine protoka, ovisno o unutarnjem promjeru kolona koje se koriste ili o uputama proizvođača kolona.
The loss is estimated at 0,2 % per hour at 30 °C.
Napomena 2.: Kod svakog prekida kolone isperite vodom.
Prior to chromatographic determination of the samples, condition the columns by repeatedly injecting the standard sample (5.4.2) in solution (8.5.1) until the area and retention time of the peak corresponding to the CMP are constant
Nikada u njima ne ostavljajte otopinu eluensa (5.2.).
Determine the response factors R by injecting the same volume of filtrates (8.5.1) as used for the samples
Prije svakog prekida koji traje više od 24 sata isperite kolone vodom i zatim ih najmanje tri sata perite otopinom (5.3.) uz brzinu protoka od 0,2 ml u minuti.
EXPRESSION OF RESULTS
Rezultati kromatografske analize ispitnog uzorka [E] dobivaju se u obliku kromatograma kod kojeg se pikovi identificiraju prema vremenu zadržavanja (RT) kako slijedi:
Method of calculation and formulae
Pik II.:
Calculation of the response factors R:
Drugi pik kromatograma s vremenom zadržavanja (RT) od oko 12,5 minuta.
R II = the response factors of peaks II,
Pik III.:
A II [0] = the areas of peaks II of the standard sample [0] obtained in 8.5.3.
Treći pik kromatograma koji odgovara CMP-u, s vremenom zadržavanja (RT) od 15,5 minuta.
R III = the response factor of peak III,
Odabir kolone ili kolona može bitno utjecati na vrijeme zadržavanja pojedinačnih pikova.
A III [0] and AIII [5] = the areas of peak III in standard samples [0] and [5] respectively obtained in 8.5.3,
Integrator (6.11.6.) automatski izračunava površinu A svakog pika:
S II [E], SIII [E], SIV [E] = the relative areas of peaks II, III and IV respectively in the sample [E],
Važno je prije kvantitativne interpretacije analizirati izgled svakog kromatograma radi otkrivanja bilo kakvih nepravilnosti nastalih zbog neispravnog rada uređaja ili kolona, ili zbog podrijetla i vrste analiziranog uzorka.
A II [E], AIII [E] = the areas of peaks II and III respectively in the sample [E] obtained in 8.4.2,
U slučaju dvojbe ponovite analizu.
R II, RIII = the response factors calculated in 9.1.1.
Kalibracija
Calculation of the relative retention time of peak III in sample [E]:
Na standardne uzorke (5.4.) točno primijenite postupak opisan od točke 8.2. do točke 8.4.2.
RRT III [E] = the relative retention time of peak III in sample [E],
Upotrebljavajte svježe pripremljene otopine budući da se u 8-postotnoj triklorooctenoj kiselini CMP razgrađuje.
RT III [E] = the retention time of peak III in sample [E] obtained in 8.4.2,
Gubitak se procjenjuje na 0,2 % na sat na temperaturi od 30 °C.
RT III [5] = the retention time of peak III in control sample [5] obtained in 8.5.3.
Prije kromatografske analize uzoraka pripremite kolone ubrizgavanjem standardnog uzorka (5.4.2.) u otopinu (8.5.1.), ponavljajući postupak sve dok se površina i vrijeme zadržavanja pika koji odgovara CMP-u ne ustale.
Experiments have shown that there is a linear relation between the relative retention time of peak III, i.e.
Faktore odziva R utvrdite ubrizgavanjem količine filtrata (8.5.1.) koja je jednaka količini uporabljenoj za uzorke.
RRTIII [E] and the percentage of whey powder added up to 10 %
PRIKAZ REZULTATA
The RRTIII [E] is < 1,000 when the whey content is > 5 %;
Metoda izračuna i formule
The RRTIII [E] is ≥ 1,000 when the whey content is ≤ 5 %.
Izračun faktora odziva R:
The uncertainty allowed for the values of RRTIII is ± 0,002.
R II = faktori odziva pikova II.;
Normally the value of RRTIII [0] deviates little from 1,034.
A II [0] = površine pikova II. standardnog uzorka [0], dobivene u 8.5.3.
Depending on the condition of the columns, the value may approach 1,000, but it shall always be greater.
R III = faktor odziva pika III.;
Calculation of the percentage of rennet whey powder in the sample:
A III [0] i AIII [5] = površine pika III. standardnih uzoraka [0] i [5], dobivene u 8.5.3.;
W = SIII[E] – [1, 3 + (SIII[0] – 0,9)]
W = količina sirutke u standardnom uzorku [5], tj.
W = the percentage m/m of rennet whey in the sample [E];
5.
S III [E] = the relative area of peak III of test sample [E] obtained as in 9.1.2;
Izračun relativne površine pikova u uzorku [E]
1,3 = represents the relative average area of peak III expressed in grams of rennet whey per 100 g determined in non-adulterated skimmed milk powder of various origins.
S II [E], SIII [E], SIV [E] = relativne površine pikova II., III. odnosno IV. u uzorku [E];
This figure was obtained experimentally;
A II [E], AIII [E] = površine pikova II. odnosno III. u uzorku [E] dobivene u 8.4.2.;
S III [0] = represents the relative area of peak III which is equal to RIII × AIII [0].
R II, RIII = faktori odziva izračunani u 9.1.1.
These values are obtained in 9.1.1 and 8.5.3 respectively;
Izračun relativnih vremena zadržavanja pika III. u uzorku [E]:
(S III [0] – 0,9) = represents the correction to be made to the relative average area 1,3 when SIII [0] is not equal to 0,9.
RRT III [E] = relativno vrijeme zadržavanja pika III. u uzorku [E];
Experimentally the relative average area of peak III of the control sample [0] is 0,9.
RT III [E] = vrijeme zadržavanja pika III. u uzorku [E] dobiveno u 8.4.2.;
Accuracy of the procedure
RT III [5] = vrijeme zadržavanja pika III. u kontrolnom uzorku [5] dobiveno u 8.5.3.
The difference between the results of two determinations carried out simultaneously or in rapid succession by the same analyst using the same apparatus on identical test material shall not exceed 0,2 % m/m.
RRTIII [E] je < 1,000 ako je sadržaj sirutke > 5 %;
The difference between two single and independent results, obtained in two different laboratories on identical test material shall not exceed 0,4 % m/m.
RRTIII [E] je ≥ 1,000 ako je sadržaj sirutke ≤ 5 %.
Interpretation
Dopuštena nepouzdanost za vrijednosti RRTIII iznosi ± 0,002.
Assume the absence of whey if the relative area of peak III, SIII [E] expressed in grams of rennet whey per 100 g of the product is ≤ 2,0 + (SIII[0] – 0,9)
U normalnim okolnostima vrijednost RRTIII [0] ne odstupa mnogo od 1,034.
where
Ovisno o stanju kolona, navedena se vrijednost može približiti 1,000, ali uvijek mora biti viša.
is the maximum value allowed for the relative area of peak III taking into account the relative average area of peak III, i.e. 1,3, the uncertainty due to variations in the composition of skimmed milk powder and the reproducibility of the method (9.3.2),
Izračun postotka slatke sirutke u prahu u uzorku:
is the correction to be made when the area SIII [0] is different from 0,9 (see point 9.2)
W = SIII[E] – [1, 3 + (SIII[0] – 0, 9)]