natgillin/translations-raw huggingface dataset:
If the relative area of peak III, SIII [E] is > 2,0 + (SIII[0] – 0,9) and the relative area of peak II, SII [E] ≤ 160, determine the rennet whey content as indicated in point 9.2.
W = maseni postotak (m/m) slatke sirutke u uzorku [E];
If the relative area of peak III, SIII [E] is > 2,0 + (SIII[0] – 0,9) and the relative area of peak II, SII [E] ≤ 160, determine the total protein content (P %); then examine graphs 1 and 2.
S III [E] = relativna površina pika III. ispitnog uzorka [E] dobivena kao u 9.1.2.;
The data obtained after analysis of samples of unadulterated skimmed milk powders with a high total protein content have been assembled in graphs 1 and 2.
1,3 = predstavlja relativnu prosječnu površinu pika III. izraženu u gramima slatke sirutke na 100 g, utvrđenu u nepatvorenom obranom mlijeku u prahu različitog podrijetla.
The continuous line represents the linear regression, the coefficients of which are calculated by the least squares method.
Ova je vrijednost dobivena eksperimentalno;
The dashed straight line fixes the upper limit of the relative area of peak III with a probability of not being exceeded in 90 % of cases.
S III [0] = predstavlja relativnu površinu pika III. koja je jednaka RIII × AIII [0].
The equations for the dashed straight lines of graphs 1 and 2 are:
Ove su vrijednosti dobivene u 9.1.1. odnosno 8.5.3.;
(graph 1),
(S III [0] – 0,9) = predstavlja korekciju relativne prosječne površine 1,3 ako SIII [0] ne iznosi 0,9.
(graph 2),
Eksperimentalno dobivena vrijednost relativne prosječne površine pika III. kontrolnog uzorka [0] iznosi 0,9.
respectively where:
Preciznost postupka
is the relative area of peak III calculated either according to total protein content or according to the relative area of peak SII [E],
Razlika između rezultata dvaju određivanja koja istodobno ili brzo jedan za drugim izvede isti analitičar koristeći se istom opremom na jednakom ispitnom materijalu ne smije biti veća od 0,2 % m/m.
P % is the total protein content expressed as a percentage, by weight,
Razlika između dva zasebna i neovisna rezultata dobivena u dva različita laboratorija na jednakom ispitnom materijalu ne smije biti veća od 0,4 % (m/m).
is the relative area of sample calculated in point 9.1.2.
Tumačenje
These equations are equivalent to the figure of 1,3 mentioned in point 9.2.
Pretpostavlja se odsutnost sirutke ako je relativna površina pika III., SIII [E], izražena u gramima slatke sirutke na 100 g proizvoda, ≤ 2,0 + (SIII [0] – 0,9),
The discrepancy (T1 and T2) between the relative area SIII [E] found and the relative area SIII is given by means of the following: T1 = SIII[E] – [(0,376 P% – 10,7) + (SIII[0] – 0,9)]T2 = SIII[E] – [(0,0123 SII[E] + 0,93) + (SIII[0] – 0,9)]
gdje je:
If T1 and/or T2
maksimalna vrijednost dopuštena za relativnu površinu pika III., uzimajući u obzir relativnu prosječnu površinu pika III., tj.
are zero or less, the presence of rennet whey cannot be determined.
1,3, nesigurnost zbog promjena u sastavu obranog mlijeka u prahu i obnovljivost metode (9.3.2.);
If T1 and T2
korekcija koju treba napraviti ako je površina SIII [0] različita od 0,9 (vidjeti točku 9.2.).
exceed zero, rennet whey is present.
Ako je relativna površina pika III., SIII [E] > 2,0 + (SIII [0] – 0,9), a relativna površina pika II., SII [E] ≤ 160, utvrdite sadržaj slatke sirutke kako je navedeno u točki 9.2.
The rennet whey content is calculated according to the following formula: W = T2 + 0,91
Ako je relativna površina pika III., SIII [E] > 2,0 + (SIII [0] – 0,9), a relativna površina pika II., SII [E] ≤ 160, utvrdite ukupni sadržaj bjelančevina (P %) te potom analizirajte dijagrame 1. i 2.
0,91 is the distance on the vertical axis between the continuous and dotted straight lines.
Podaci dobiveni analizom uzoraka nepatvorenog obranog mlijeka u prahu s visokim ukupnim sadržajem bjelančevina sabrani su u dijagramima 1. i 2.
Peak II
Puna linija predstavlja lineranu regresiju za koju se koeficijenti računaju metodom najmanjih kvadrata.
Skimmed milk powder
Isprekidana ravna linija označava gornju graničnu vrijednost relativne površine pika III., uz vjerojatnost da u 90 % slučajeva nije prekoračena.
total protein content (%)
Jednadžbe isprekidanih ravnih linija u dijagramima 1. i 2. jesu sljedeće:
Peak III
(dijagram 1.)
Appendix III
(dijagram 2.)
DETERMINING RENNET WHEY SOLIDS IN SKIMMED MILK POWDER
gdje je:
PURPOSE: DETECTING THE ADDITION OF RENNET WHEY SOLIDS TO SKIMMED MILK POWDER
relativna površina pika III. izračunana ili prema ukupnom sadržaju bjelančevina ili prema relativnoj površini pika SII [E];
The content of rennet whey solids is defined as the percentage by mass as determined by caseinomacropeptide content by the procedure described.
relativna površina uzorka izračunana u točki 9.1.2.
Samples are analysed for caseinomacropeptide A by a reversed-phase high-performance liquid chromatography procedure (HPLC procedure).
Ove su jednadžbe ekvivalentne vrijednosti 1,3 spomenutoj u točki 9.2.
Evaluation of the result is obtained by reference to standard samples consisting of skimmed milk powder with and without a known percentage of whey powder.
Razlika (T1 i T2) između relativne površine SIII [E] i relativne površine SIII prikazana je sljedećom formulom: T1 = SIII[E] – [(0,376 P% – 10,7) + (SIII[0] – 0,9)]T2 = SIII[E] – [(0,0123 SII[E] + 0,93) + (SIII[0] – 0,9)]
Results higher than 1 % (m/m) show that rennet whey solids are present.
Ako su T1 i/ili T2
Acetonitrile should be of spectroscopic or HPLC quality.
jednaki ili manji od nule, prisutnost slatke sirutke nije moguće utvrditi.
Eluents A and B
Ako su T1 i T2
Eluent A: 150 ml of acetonitrile (CH3CN), 20 ml of isopropanol (CH3CHOHCH3), and 1,00 ml of trifluoroacetic acid (TFA, CF3COOH) are placed in a 1000 ml volumetric flask.
veći od nule, slatka sirutka je prisutna.
Make up to 1000 ml with water.
Sadržaj slatke sirutke računa se prema sljedećoj formuli: W = T2 + 0,91
Eluent B: 550 ml of acetonitrile, 20 ml of isopropanol and 1,00 ml of TFA are placed in a 1000 ml volumetric flask.
0,91 udaljenost na okomitoj osi između pune i isprekidane ravne linije.
Conservation of the column
Pik II.
After the analyses the column is flushed with eluent B (via a gradient) and subsequently flushed with acetonitrile (via a gradient for 30 minutes).
Obrano mlijeko u prahu
The column is stored in acetonitrile.
ukupni sadržaj bjelančevina (%)
Skimmed milk powder meeting the requirements for public storage (i.e. [0]).
Pik III.:
The same skimmed milk powder adulterated with 5 % (m/m) rennet-type whey powder of standard composition (i.e. [5]).
Dodatak III.
The same skimmed milk powder adulterated with 50 % (m/m) rennet-type whey powder of standard composition (i.e. [50])
ODREĐIVANJE SUHE TVARI SLATKE SIRUTKE U OBRANOM MLIJEKU U PRAHU
Graduated pipettes, allowing delivery of 10 ml (ISO 648, Class A, or ISO/R 835), or a dispensing system capable of delivering 10,0 ml in two minutes
SVRHA: DETEKCIJA DODANE SUHE TVARI SLATKE SIRUTKE U:
Binary gradient pumping system
REFERENCA: MEĐUNARODNA NORMA ISO 707
Injector, hand or automatic, with a 100 μl capacity
Udio suhe tvari slatke sirutke definira se kao maseni postotak kazeino-makropeptida prema opisanom postupku.
Agilent Technologies Zorbax 300 SB-C3 column (length 25 cm, 0,46 cm internal diameter) or an equivalent wide-pore silica based reversed-phase column
Uzorci se analiziraju na prisutnost kazeino-makropeptida A metodom reverzno-fazne tekućinske kromatografije visoke djelotvornosti (HPLC postupak).
Variable wavelength UV detector, permitting measurements at 210 nm (if necessary, a higher wavelength up to 220 nm may be used) with a sensitivity of 0,02 Å
Dobiveni se rezultat ocjenjuje uspoređivanjem sa standardnim uzorcima koji se sastoje od obranog mlijeka u prahu sa i bez dodatka poznatog postotka sirutke u prahu.
Integrator capable of setting the integration to common baseline or valley-to-valley
Rezultati iznad 1 % (m/m) ukazuju na prisutnost suhe tvari slatke sirutke.
Note: Operation of the column at room temperature is possible, provided that the room temperature does not fluctuate more than 1 °C, otherwise too much variation in the retention time of CMPA takes place.
Acetonitril treba biti spektroskopske ili HPLC čistoće.
Store the sample in conditions which preclude any deterioration or change in composition.
Eluensi A i B
Weigh 2,00 ± 0,001 g of test sample into a centrifuge tube (6.2) or suitable stoppered flask (50 ml).
Eluens A: U odmjernu tikvicu od 1000 ml stavite 150 ml acetonitrila (CH3CN), 20 ml izopropanola (CH3CHOHCH3) i 1,00 ml trifluorooctene kiseline (TFA, CF3COOH).
Note: In the case of mixtures, weigh such an amount of the test sample that the defatted sample portion corresponds to 2,00 g.
Dopunite vodom do 1000 ml.
Add 10,0 ml of the trichloroacetic acid solution of ca.
Dopunite vodom do 1000 ml.
25 °C (5.1) constantly over two minutes, while stirring vigorously with the aid of the magnetic stirrer (6.4).
Konzerviranje kolone
Centrifuge (6.2) 2200 g for 10 minutes, or filter through paper (6.6), discarding the first 5 ml of filtrate
Nakon analize kolona se najprije ispere eluensom B (gradijentno ispiranje), a potom i acetonitrilom (gradijentno ispiranje u trajanju od 30 minuta).
The reversed-phase HPLC method excludes the possibility false-positive results due to the presence of acid buttermilk powder.
Kolona se čuva u acetonitrilu.
Before the reversed phase HPLC-analysis is carried out, the gradient conditions should be optimised.
Obrano mlijeko u prahu koje ispunjava zahtjeve za javno skladištenje (tj.
A retention time of 26 ± 2 minutes for CMPA is optimal for gradient systems having a dead volume of about 6 ml (volume from the point where the solvents come together to the volume of the injector loop, inclusive).
[0]).
Gradient systems having a lower dead volume (e.g. 2 ml) should use 22 minutes as an optimal retention time
Isto mlijeko u prahu s dodatkom 5 % (m/m) slatke sirutke u prahu standardnog sastava (tj.
Take solutions of the standard samples (5.4) without and with 50 % rennet whey.
[5]).
Inject 100 μl of supernatant or filtrate (8.3.3) into the HPLC apparatus operating at the scouting gradient conditions given in Table 1.
Isto mlijeko u prahu s dodatkom 50 % (m/m) slatke sirutke u prahu standardnog sastava (tj.
Table 1
[50]).
Scouting gradient conditions for optimisation of the chromatography
Graduirane pipete koje omogućuju prijenos 10 ml (ISO 648, Klasa A ili ISO/R 835) ili dozirni sustav koji omogućuje unos 10,0 ml u dvije minute
Time
Pumpni sustav s binarnim gradijentom
Flow
Injektor, ručni ili automatski, zapremine 100 μl
Curve
Kolona Agilent Technologies Zorbax 300 SB-C3 (duljine 25 cm, unutarnjeg promjera 0,46 cm) ili ekvivalentna reverzno-fazna kolona sa širokim porama na bazi silicijeva dioksida
linear
UV detektor promjenjive valne duljine koji omogućuje mjerenja na 210 nm (prema potrebi se mogu koristiti i veće valne duljine do 220 nm), osjetljivosti od 0,02 Å
Comparison of the two chromatograms should reveal the location of the peak of CMPΑ.
Integrator koji omogućuje integraciju na zajedničkoj baznoj liniji ili na dva minimuma („dolina–dolina”).
Using the formula given below, the initial solvent composition to be used for the normal gradient (see 8.4.3) can be calculated % B = 10 – 2,5 + (13,5 + (RTcmpA – 26) / 6) * 30 / 27 % B = 7,5 + (13,5 + (RTcmpA – 26) / 6) * 1,11
Napomena: Kolona se može upotrebljavati na sobnoj temperaturi, uz uvjet da temperatura u prostoriji ne varira za više od 1 °C; u protivnom dolazi do prevelikog variranja vremena zadržavanja CMPA.
Where:
Uzorke čuvajte u uvjetima koji onemogućavaju kvarenje ili promjene u njihovu sastavu.
RT cmpA : retention time of CMPΑ in the scouting gradient
Odvagnite 2,00 + 0,001 g ispitnog uzorka u epruvetu za centrifugiranje (6.2.) ili u odgovarajuću tikvicu s čepom (50 ml).
10 : the initial % B of the scouting gradient
Napomena: Kad se radi o smjesi, odvagnite takvu količinu ispitnog uzorka da odmašćeni uzorak za analizu iznosi 2,00 g.
2,5 : % B at midpoint minus % B at initial in the normal gradient
Dodajte 10,0 ml otopine triklorooctene kiseline zagrijane na oko 25 °C (5.1.), postupno u dvije minute, uz snažno miješanje magnetskom miješalicom (6.4.).
13,5 : midpoint time of the scouting gradient
Centrifugirajte (6.2.) 10 minuta na 2200 g ili profiltrirajte kroz filtar-papir (6.6.), pri čemu prvih 5 ml filtrata bacite.
26 : required retention time of CMPΑ
Reverzno-fazna HPLC metoda isključuje mogućnost lažno pozitivnih rezultata zbog prisutnosti kisele mlaćenice u prahu.
6 : ratio of slopes of the scouting and normal gradient
Prije reverzno-fazne HPLC analize treba optimizirati uvjete gradijenta.
Take solutions of the test samples
2 ml) iznosi 22 minute.
Inject 100 μl of accurately measured supernatant or filtrate (8.3.3) into the HPLC apparatus operating at a flow rate of 1,0 ml of eluent solution (5.2) per minute.
Uzmite otopine standardnih uzoraka (5.4.) sa 50 %-tnom slatkom sirutkom i bez nje.
Immediately after the injection a linear gradient is started, which results in a 5 % higher percentage of B after 27 minutes.
Uvjeti referentnog gradijenta za optimizaciju kromatografije
Subsequently a linear gradient is started, which brings the eluent composition to 90 % B in five minutes.
Vrijeme
Note 1.
linearna
The retention time of the CMPA should be 26 ± 2 minutes.
Usporedba dvaju kromatograma trebala bi otkriti položaj pika CMPA.
This can be achieved by varying the initial and end conditions of the first gradient.
Sastav otopine koju treba koristiti za normalni gradijent (vidjeti 8.4.3.) može se izračunati prema sljedećoj formuli: % B = 10 – 2,5 + (13,5 + (RTcmpA – 26) / 6) × 30 / 27 % B = 7,5 + (13,5 + (RTcmpA – 26) / 6) × 1,11
However, the difference in the % B for the initial and end conditions of the first gradient shall remain 5 % B.
gdje je:
Note 2.
RT cmpA : vrijeme zadržavanja CMPA u referentnom gradijentu;
The eluents should be degassed sufficiently and should also remain degassed.
10 : početni % B referentnog gradijenta;
This is essential for proper functioning of the gradient pumping system.
2,5 : % B u središnjoj točki minus % B na početku u normalnom gradijentu;
The standard deviation for the retention time of the CMPA peak should be smaller than 0,1 minutes (n = 10).
13,5 : vrijeme u središnjoj točki referentnog gradijenta;
Note 3.
26 : potrebno vrijeme zadržavanja CMPA;
Every five samples the reference sample [5] should be injected and used to calculate a new response factor R. (9.1.1).
6 : omjer nagiba referentnog gradijenta i normalnog gradijenta;
The results of the chromatographic analysis of the test sample (E) are obtained in the form of a chromatogram in which the CMPA peak is identified by its retention time of about 26 minutes
30 : % B na početku minus % B nakon 27 minuta u referentnom gradijentu;
The integrator (6.11.6) automatically calculates the peak height H of the CMPA peak.
27 : vrijeme primjene referentnog gradijenta.
The baseline location should be checked in every chromatogram.
Uzmite otopine ispitnih uzoraka.
The analysis or the integration should be repeated if the baseline was incorrectly located.
Ubrizgajte 100 μl točno izmjerenog supernatanta ili filtrata (8.3.3.) u HPLC uređaj koji radi uz brzinu protoka od 1,0 ml otopine eluensa (5.2.) u minuti.
Note: If the CMPA peak is sufficiently separated from other peaks valley-to-valley baseline allocation should be used, otherwise use dropping perpendiculars to a common baseline, which should have starting point close to the CMPA peak (thus not at t = 0 min!).Use for the standard and the samples the same type integration type and check in case of common baseline its consistency for the samples and the standard.
Sastav eluensa na početku analize dobiva se na način opisan u točki 8.4.2.
It is essential to examine the appearance of each chromatogram prior to quantitative interpretation, in order to detect any abnormalities due either to malfunctioning of the apparatus or the column, or to the origin and nature of the sample analysed.
On obično približno odgovara omjeru A:B = 76:24 (5.2.).
Apply exactly the procedure described from point 8.2 to point 8.4.4 to the standard samples (5.4.1 to 5.4.2).
Odmah nakon ubrizgavanja pokreće se linearni gradijent, posljedica čega je 5 % viši postotak od B nakon 27 minuta.
Use freshly prepared solutions, because CMP degrades in an 8 % trichloroacetic acid environment at room temperature.
Potom se pokreće linearni gradijent koji u pet minuta mijenja sastav eluensa u 90 % B.
At 4 °C the solution remains stable for 24 hours.
Taj se sastav održava pet minuta, nakon čega se pomoću linearnog gradijenta mijenja, da bi se nakon pet minuta ponovo vratio na početni sastav.
In the case of long series of analyses the use of a cooled sample tray in the automatic injector is desirable
Ovisno o unutarnjem volumenu pumpnog sustava, sljedeće se ubrizgavanje može izvršiti 15 minuta nakon postizanja početnih uvjeta.
Note: 8.4.2. may be omitted if the % B at initial conditions is known from previous analyses.
To je moguće postići mijenjanjem početnih i konačnih uvjeta prvog gradijenta.
The chromatogram of the reference sample [5] should be analogous to Figure.
Međutim, razlika u % B između početnih i konačnih uvjeta prvog gradijenta mora ostati 5 % B.
In this figure the CMPA peak is preceded by two small peaks.
To je od ključne važnosti za pravilan rad gradijentnog pumpnog sustava.
It is essential to obtain a similar separation.
Standardna devijacija vremena zadržavanja pika CMPA mora biti manja od 0,1 minute (n = 10).
Prior to chromatographic determination of the samples inject 100 μl of the standard sample without rennet whey [0] (5.4.1)
Rezultati kromatografske analize ispitnog uzorka (E) dobiju se u obliku kromatograma u kojem se pik CMPA utvrđuje na temelju vremena zadržavanja koje iznosi oko 26 minuta.
The chromatogram should not show a peak at the retention time of the CMPA peak.
Integrator (6.11.6.) automatski izračunava visinu H pika CMPA.
Determine the response factors R by injecting the same volume of filtrate (8.5.1) as used for the samples.
Na svakom kromatogramu treba provjeriti položaj bazne linije.
Calculation of the response factor R:
Analizu ili integriranje treba ponoviti ako bazna linija nije pravilno smještena.
CMPA peak: R = W/H
Napomena: Ako je pik CMPA dovoljno odvojen od drugih pikova, treba koristiti alokaciju bazne linije dolina-dolina, u suprotnom slučaju koristite padajuće okomice na baznu liniju, koje trebaju imati ishodište blizu pika CMPA (prema tome ne kad je t = 0 min!).
R = the response factor of the CMPA peak
Za standard i uzorke primjenjujte istu vrstu integracije te u slučaju zajedničke bazne linije provjerite njezinu konzistentnost za uzorke i standard.
H = the height of the CMPA peak
Prije kvantitativne interpretacije od ključne je važnosti analizirati izgled svakog kromatograma radi otkrivanja bilo kakvih nepravilnosti nastalih zbog neispravnog rada uređaja ili kolona, ili zbog podrijetla i vrste analiziranog uzorka.
W = the quantity of whey in the standard sample [5].
Na standardne uzorke (od 5.4.1. do 5.4.2.) točno primijenite postupak opisan od točke 8.2. do točke 8.4.4.
H(E) = the height of the CMPA peak of the sample (E)
Napomena: točka 8.4.2. može se izostaviti ako je % B u početnim uvjetima poznat iz prethodnih analiza.
If W(E) is greater than 1 % and the difference between the retention time and that of the standard sample [5] is smaller than 0,2 minutes then rennet whey solids are present.
Kromatogram referentnog uzorka [5] mora biti analogan slici 1.
Not determined.
Na toj slici piku CMPA prethode dva manja pika.
Linearity
Ključno je dobiti slično razdvajanje.
From 0 to 16 % of rennet whey a linear relationship should be obtained with a coefficient of correlation > 0,99.
Prije kromatografskog određivanja uzoraka ubrizgajte 100 μl standardnog uzorka bez slatke sirutke [0] (5.4.1.).
The 1 % limit includes the uncertainty due to reproducibility.
Kromatogram ne smije pokazivati pik u vremenu zadržavanja pika CMPA.
Ni—4.6 standard
Odredite faktore odziva R ubrizgavanjem količine filtrata (8.5.1.) jednake količini koja je korištena za uzorke.
Absorbance (220 nm)
Izračun faktora odziva R:
time (Minutes)
Pik CMPA: R = W/H
International IDF Standard 135B/1991.
R = faktor odziva za pik CMPA;
Milk and milk products.
H = visina pika CMPA;
Precision characteristics of analytical methods.
W = količina sirutke u standardnom uzorku [5].
Outline of collaborative study procedure.’
Izračun postotka slatke sirutke u prahu u uzorku
the following Annexes are added:
W(E) = postotak (m/m) slatke sirutke u uzorku (E);
ANNEX VI
R = faktor odziva za pik CMPA (9.1.1.);
Methods of analysis of butter under private storage
H(E) = visina pika CMPA uzorka (E).
Non Fat Solids (excluding salt)
Ako je W(E) veći od 1 % i ako je razlika između vremena zadržavanja i vremena standardnog uzorka [5] manja od 0,2 minute, to ukazuje na prisutnost suhe tvari slatke sirutke.
Salt
Nije određena.
The method to be applied shall be approved by the paying agency.
Linearnost
ANNEX VII
Od 0 do 16 % slatke sirutke linearni se odnos treba dobiti s korelacijskim koeficijentom > 0,99.
Methods of analysis of skimmed milk powder under private storage
Granica od 1 % uključuje nesigurnost zbog obnovljivosti.
ANNEX VIII
Ni—4.6 standard vrijeme (u minutama)
Methods of analysis of cheeses under private storage
Obilježja preciznosti metoda analize.
The method of analysis laid down in the Appendix shall be used to ensure that cheese made exclusively from ewe's milk, goat's milk or buffalo milk or from a mixture of ewe's milk, goat's milk and buffalo milk does not contain cow's milk casein.
Prikaz postupka međulaboratorijske studije.”
Cow's milk casein is considered to be present if the cow's milk casein content of the analysed sample is equal to or higher than the content of the reference sample containing 1 % cow's milk as laid down in the Appendix.
Dodaju se sljedeći prilozi:
Methods for detecting cow's milk casein in cheeses referred to in paragraph 1 may be used provided that:
PRILOG VI.
the detection limit is maximum 0,5 % and
Metode analize maslaca u privatnom skladištenju
there are no false-positive results and
Bezmasna suha tvar (bez soli)
cow's milk casein is detectable with the required sensitivity even after long ripening periods, as may occur in usual commercial conditions.
Sol
If any of the above mentioned requirements is not met, the methods laid down in the Appendix shall be used.
Metodu koja se primjenjuje odobrava agencija za plaćanja.
Appendix
PRILOG VII.
METHOD FOR THE DETECTION OF COW'S MILK AND CASEINATE IN CHEESES FROM EWE'S MILK, GOAT'S MILK OR BUFFALO MILK OR MIXTURES OF EWE'S MILK, GOAT'S MILK AND BUFFALO MILK
Metode analize obranog mlijeka u prahu u privatnom skladištenju
SCOPE
PRILOG VIII.
Detection of cow's milk and caseinate in cheeses made from ewe's milk, goat's milk, buffalo milk or mixtures of ewe's, goat's and buffalo milk by isoelectric focusing of γ-caseins after plasminolysis.
Metode analize sira u privatnom skladištenju
FIELD OF APPLICATION
Metoda analize utvrđena u Dodatku upotrebljava se kako bi se osiguralo da sir napravljen isključivo od ovčjeg, kozjeg ili bivoljeg mlijeka ili od mješavine ovčjeg, kozjeg ili bivoljeg mlijeka ne sadržava kazein kravljeg mlijeka.
The method is suitable for sensitive and specific detection of native and heat-treated cow's milk and caseinate in fresh and ripened cheeses made from ewe's milk, goat's milk, buffalo milk or mixtures of ewe's, goat's and buffalo milk.
Ako je udio kazeina kravljeg mlijeka u analiziranom uzorku jednak udjelu u referentnom uzorku koji sadržava 1 % kravljeg mlijeka ili viši od njega, kako je utvrđeno u Dodatku, smatra se da je kazein kravljeg mlijeka prisutan.
It is not suitable for the detection of milk and cheese adulteration by heat-treated bovine whey protein concentrates.
Metode za detekciju kazeina kravljeg mlijeka u sirevima iz stavka 1. mogu se primijeniti pod uvjetom da:
Isolation of caseins from cheese and the reference standards
granica detekcije iznosi maksimalno 0,5 %;
Dissolving of the isolated caseins and submitting to plasmin (EC.3.4.21.7) cleavage
nema lažno pozitivnih rezultata;