natgillin/translations-raw huggingface dataset:
Assume the absence of whey if the relative area of peak III, SIII [E] expressed in grams of rennet whey per 100 g of the product is ≤ 2,0 + (SIII[0] – 0,9)
Pretpostavlja se odsutnost sirutke ako je relativna površina pika III., SIII [E], izražena u gramima slatke sirutke na 100 g proizvoda, ≤ 2,0 + (SIII [0] – 0,9),
is the maximum value allowed for the relative area of peak III taking into account the relative average area of peak III, i.e. 1,3, the uncertainty due to variations in the composition of skimmed milk powder and the reproducibility of the method (9.3.2),
maksimalna vrijednost dopuštena za relativnu površinu pika III., uzimajući u obzir relativnu prosječnu površinu pika III., tj. 1,3, nesigurnost zbog promjena u sastavu obranog mlijeka u prahu i obnovljivost metode (9.3.2.);
is the correction to be made when the area SIII [0] is different from 0,9 (see point 9.2)
korekcija koju treba napraviti ako je površina SIII [0] različita od 0,9 (vidjeti točku 9.2.).
If the relative area of peak III, SIII [E] is > 2,0 + (SIII[0] – 0,9) and the relative area of peak II, SII [E] ≤ 160, determine the rennet whey content as indicated in point 9.2.
Ako je relativna površina pika III., SIII [E] > 2,0 + (SIII [0] – 0,9), a relativna površina pika II., SII [E] ≤ 160, utvrdite sadržaj slatke sirutke kako je navedeno u točki 9.2.
If the relative area of peak III, SIII [E] is > 2,0 + (SIII[0] – 0,9) and the relative area of peak II, SII [E] ≤ 160, determine the total protein content (P %); then examine graphs 1 and 2.
Ako je relativna površina pika III., SIII [E] > 2,0 + (SIII [0] – 0,9), a relativna površina pika II., SII [E] ≤ 160, utvrdite ukupni sadržaj bjelančevina (P %) te potom analizirajte dijagrame 1. i 2.
The data obtained after analysis of samples of unadulterated skimmed milk powders with a high total protein content have been assembled in graphs 1 and 2.
Podaci dobiveni analizom uzoraka nepatvorenog obranog mlijeka u prahu s visokim ukupnim sadržajem bjelančevina sabrani su u dijagramima 1. i 2.
The continuous line represents the linear regression, the coefficients of which are calculated by the least squares method.
Puna linija predstavlja lineranu regresiju za koju se koeficijenti računaju metodom najmanjih kvadrata.
The dashed straight line fixes the upper limit of the relative area of peak III with a probability of not being exceeded in 90 % of cases.
Isprekidana ravna linija označava gornju graničnu vrijednost relativne površine pika III., uz vjerojatnost da u 90 % slučajeva nije prekoračena.
The equations for the dashed straight lines of graphs 1 and 2 are:
Jednadžbe isprekidanih ravnih linija u dijagramima 1. i 2. jesu sljedeće:
(graph 1),
(dijagram 1.)
(graph 2),
(dijagram 2.)
respectively where:
gdje je:
is the relative area of peak III calculated either according to total protein content or according to the relative area of peak SII [E],
relativna površina pika III. izračunana ili prema ukupnom sadržaju bjelančevina ili prema relativnoj površini pika SII [E];
P % is the total protein content expressed as a percentage, by weight,
P % ukupni sadržaj bjelančevina izražen kao maseni postotak;
is the relative area of sample calculated in point 9.1.2.
relativna površina uzorka izračunana u točki 9.1.2.
These equations are equivalent to the figure of 1,3 mentioned in point 9.2.
Ove su jednadžbe ekvivalentne vrijednosti 1,3 spomenutoj u točki 9.2.
The discrepancy (T1 and T2) between the relative area SIII [E] found and the relative area SIII is given by means of the following: T1 = SIII[E] – [(0,376 P% – 10,7) + (SIII[0] – 0,9)]T2 = SIII[E] – [(0,0123 SII[E] + 0,93) + (SIII[0] – 0,9)]
Razlika (T1 i T2) između relativne površine SIII [E] i relativne površine SIII prikazana je sljedećom formulom: T1 = SIII[E] – [(0,376 P% – 10,7) + (SIII[0] – 0,9)]T2 = SIII[E] – [(0,0123 SII[E] + 0,93) + (SIII[0] – 0,9)]
If T1 and/or T2
Ako su T1 i/ili T2
are zero or less, the presence of rennet whey cannot be determined.
jednaki ili manji od nule, prisutnost slatke sirutke nije moguće utvrditi.
If T1 and T2
Ako su T1 i T2
exceed zero, rennet whey is present.
veći od nule, slatka sirutka je prisutna.
The rennet whey content is calculated according to the following formula: W = T2 + 0,91
Sadržaj slatke sirutke računa se prema sljedećoj formuli: W = T2 + 0,91
0,91 is the distance on the vertical axis between the continuous and dotted straight lines.
0,91 udaljenost na okomitoj osi između pune i isprekidane ravne linije.
Peak II
Pik II.
total protein content (%)
ukupni sadržaj bjelančevina (%)
Peak III
Pik III.:
DETERMINING RENNET WHEY SOLIDS IN SKIMMED MILK POWDER
ODREĐIVANJE SUHE TVARI SLATKE SIRUTKE U OBRANOM MLIJEKU U PRAHU
PURPOSE: DETECTING THE ADDITION OF RENNET WHEY SOLIDS TO SKIMMED MILK POWDER
SVRHA: DETEKCIJA DODANE SUHE TVARI SLATKE SIRUTKE U:
REFERENCES: INTERNATIONAL STANDARD ISO 707
REFERENCA: MEĐUNARODNA NORMA ISO 707
The content of rennet whey solids is defined as the percentage by mass as determined by caseinomacropeptide content by the procedure described.
Udio suhe tvari slatke sirutke definira se kao maseni postotak kazeino-makropeptida prema opisanom postupku.
Samples are analysed for caseinomacropeptide A by a reversed-phase high-performance liquid chromatography procedure (HPLC procedure).
Uzorci se analiziraju na prisutnost kazeino-makropeptida A metodom reverzno-fazne tekućinske kromatografije visoke djelotvornosti (HPLC postupak).
Evaluation of the result is obtained by reference to standard samples consisting of skimmed milk powder with and without a known percentage of whey powder.
Dobiveni se rezultat ocjenjuje uspoređivanjem sa standardnim uzorcima koji se sastoje od obranog mlijeka u prahu sa i bez dodatka poznatog postotka sirutke u prahu.
Results higher than 1 % (m/m) show that rennet whey solids are present.
Rezultati iznad 1 % (m/m) ukazuju na prisutnost suhe tvari slatke sirutke.
Acetonitrile should be of spectroscopic or HPLC quality.
Acetonitril treba biti spektroskopske ili HPLC čistoće.
Eluents A and B
Eluensi A i B
Eluent A: 150 ml of acetonitrile (CH3CN), 20 ml of isopropanol (CH3CHOHCH3), and 1,00 ml of trifluoroacetic acid (TFA, CF3COOH) are placed in a 1000 ml volumetric flask.
Eluens A: U odmjernu tikvicu od 1000 ml stavite 150 ml acetonitrila (CH3CN), 20 ml izopropanola (CH3CHOHCH3) i 1,00 ml trifluorooctene kiseline (TFA, CF3COOH). Dopunite vodom do 1000 ml.
Make up to 1000 ml with water.
Dopunite vodom do 1000 ml.
Conservation of the column
Konzerviranje kolone
After the analyses the column is flushed with eluent B (via a gradient) and subsequently flushed with acetonitrile (via a gradient for 30 minutes).
Nakon analize kolona se najprije ispere eluensom B (gradijentno ispiranje), a potom i acetonitrilom (gradijentno ispiranje u trajanju od 30 minuta).
The column is stored in acetonitrile.
Kolona se čuva u acetonitrilu.
Skimmed milk powder meeting the requirements for public storage (i.e. [0]).
Obrano mlijeko u prahu koje ispunjava zahtjeve za javno skladištenje (tj. [0]).
The same skimmed milk powder adulterated with 5 % (m/m) rennet-type whey powder of standard composition (i.e. [5]).
Isto mlijeko u prahu s dodatkom 5 % (m/m) slatke sirutke u prahu standardnog sastava (tj. [5]).
The same skimmed milk powder adulterated with 50 % (m/m) rennet-type whey powder of standard composition (i.e. [50])
Isto mlijeko u prahu s dodatkom 50 % (m/m) slatke sirutke u prahu standardnog sastava (tj. [50]).
Graduated pipettes, allowing delivery of 10 ml (ISO 648, Class A, or ISO/R 835), or a dispensing system capable of delivering 10,0 ml in two minutes
Graduirane pipete koje omogućuju prijenos 10 ml (ISO 648, Klasa A ili ISO/R 835) ili dozirni sustav koji omogućuje unos 10,0 ml u dvije minute
Binary gradient pumping system
Pumpni sustav s binarnim gradijentom
Injector, hand or automatic, with a 100 μl capacity
Injektor, ručni ili automatski, zapremine 100 μl
Agilent Technologies Zorbax 300 SB-C3 column (length 25 cm, 0,46 cm internal diameter) or an equivalent wide-pore silica based reversed-phase column
Kolona Agilent Technologies Zorbax 300 SB-C3 (duljine 25 cm, unutarnjeg promjera 0,46 cm) ili ekvivalentna reverzno-fazna kolona sa širokim porama na bazi silicijeva dioksida
Variable wavelength UV detector, permitting measurements at 210 nm (if necessary, a higher wavelength up to 220 nm may be used) with a sensitivity of 0,02 Å
UV detektor promjenjive valne duljine koji omogućuje mjerenja na 210 nm (prema potrebi se mogu koristiti i veće valne duljine do 220 nm), osjetljivosti od 0,02 Å
Integrator capable of setting the integration to common baseline or valley-to-valley
Integrator koji omogućuje integraciju na zajedničkoj baznoj liniji ili na dva minimuma („dolina–dolina”).
Note: Operation of the column at room temperature is possible, provided that the room temperature does not fluctuate more than 1 °C, otherwise too much variation in the retention time of CMPA takes place.
Napomena: Kolona se može upotrebljavati na sobnoj temperaturi, uz uvjet da temperatura u prostoriji ne varira za više od 1 °C; u protivnom dolazi do prevelikog variranja vremena zadržavanja CMPA.
Store the sample in conditions which preclude any deterioration or change in composition.
Uzorke čuvajte u uvjetima koji onemogućavaju kvarenje ili promjene u njihovu sastavu.
Weigh 2,00 ± 0,001 g of test sample into a centrifuge tube (6.2) or suitable stoppered flask (50 ml).
Odvagnite 2,00 + 0,001 g ispitnog uzorka u epruvetu za centrifugiranje (6.2.) ili u odgovarajuću tikvicu s čepom (50 ml).
Note: In the case of mixtures, weigh such an amount of the test sample that the defatted sample portion corresponds to 2,00 g.
Napomena: Kad se radi o smjesi, odvagnite takvu količinu ispitnog uzorka da odmašćeni uzorak za analizu iznosi 2,00 g.
Add 10,0 ml of the trichloroacetic acid solution of ca. 25 °C (5.1) constantly over two minutes, while stirring vigorously with the aid of the magnetic stirrer (6.4).
Dodajte 10,0 ml otopine triklorooctene kiseline zagrijane na oko 25 °C (5.1.), postupno u dvije minute, uz snažno miješanje magnetskom miješalicom (6.4.).
Centrifuge (6.2) 2200 g for 10 minutes, or filter through paper (6.6), discarding the first 5 ml of filtrate
Centrifugirajte (6.2.) 10 minuta na 2200 g ili profiltrirajte kroz filtar-papir (6.6.), pri čemu prvih 5 ml filtrata bacite.
The reversed-phase HPLC method excludes the possibility false-positive results due to the presence of acid buttermilk powder.
Reverzno-fazna HPLC metoda isključuje mogućnost lažno pozitivnih rezultata zbog prisutnosti kisele mlaćenice u prahu.
Before the reversed phase HPLC-analysis is carried out, the gradient conditions should be optimised.
Prije reverzno-fazne HPLC analize treba optimizirati uvjete gradijenta.
A retention time of 26 ± 2 minutes for CMPA is optimal for gradient systems having a dead volume of about 6 ml (volume from the point where the solvents come together to the volume of the injector loop, inclusive).
Vrijeme zadržavanja od 26 ± 2 minute za CMPA optimalno je za gradijentne sustave s mrtvim volumenom od oko 6 ml (volumen od točke gdje se otopine pomiješaju do volumena petlje injektora, uključujući i njega).
Gradient systems having a lower dead volume (e.g. 2 ml) should use 22 minutes as an optimal retention time
Optimalno vrijeme zadržavanja za gradijentne sustave s nižim mrtvim volumenom (npr. 2 ml) iznosi 22 minute.
Take solutions of the standard samples (5.4) without and with 50 % rennet whey.
Uzmite otopine standardnih uzoraka (5.4.) sa 50 %-tnom slatkom sirutkom i bez nje.
Inject 100 μl of supernatant or filtrate (8.3.3) into the HPLC apparatus operating at the scouting gradient conditions given in Table 1.
Ubrizgajte 100 μl supernatanta ili filtrata (8.3.3.) u HPLC uređaj koji funkcionira u uvjetima referentnog gradijenta navedenim u tablici 1.
Scouting gradient conditions for optimisation of the chromatography
Uvjeti referentnog gradijenta za optimizaciju kromatografije
linear
linearna
Comparison of the two chromatograms should reveal the location of the peak of CMPΑ.
Usporedba dvaju kromatograma trebala bi otkriti položaj pika CMPA.
Using the formula given below, the initial solvent composition to be used for the normal gradient (see 8.4.3) can be calculated % B = 10 – 2,5 + (13,5 + (RTcmpA – 26) / 6) * 30 / 27 % B = 7,5 + (13,5 + (RTcmpA – 26) / 6) * 1,11
Sastav otopine koju treba koristiti za normalni gradijent (vidjeti 8.4.3.) može se izračunati prema sljedećoj formuli: % B = 10 – 2,5 + (13,5 + (RTcmpA – 26) / 6) × 30 / 27 % B = 7,5 + (13,5 + (RTcmpA – 26) / 6) × 1,11
RT cmpA : retention time of CMPΑ in the scouting gradient
RT cmpA : vrijeme zadržavanja CMPA u referentnom gradijentu;
10 : the initial % B of the scouting gradient
10 : početni % B referentnog gradijenta;
2,5 : % B at midpoint minus % B at initial in the normal gradient
2,5 : % B u središnjoj točki minus % B na početku u normalnom gradijentu;
13,5 : midpoint time of the scouting gradient
13,5 : vrijeme u središnjoj točki referentnog gradijenta;
26 : required retention time of CMPΑ
26 : potrebno vrijeme zadržavanja CMPA;
6 : ratio of slopes of the scouting and normal gradient
6 : omjer nagiba referentnog gradijenta i normalnog gradijenta;
30 : % B at initial minus % B at 27 minutes in the scouting gradient
30 : % B na početku minus % B nakon 27 minuta u referentnom gradijentu;
27 : run-time of the scouting gradient.
27 : vrijeme primjene referentnog gradijenta.
Take solutions of the test samples
Uzmite otopine ispitnih uzoraka.
Inject 100 μl of accurately measured supernatant or filtrate (8.3.3) into the HPLC apparatus operating at a flow rate of 1,0 ml of eluent solution (5.2) per minute.
Ubrizgajte 100 μl točno izmjerenog supernatanta ili filtrata (8.3.3.) u HPLC uređaj koji radi uz brzinu protoka od 1,0 ml otopine eluensa (5.2.) u minuti.
The composition of the eluent of the start of the analysis is obtained from 8.4.2.
Sastav eluensa na početku analize dobiva se na način opisan u točki 8.4.2.
It is normally close to A:B = 76:24 (5.2).
On obično približno odgovara omjeru A:B = 76:24 (5.2.).
Immediately after the injection a linear gradient is started, which results in a 5 % higher percentage of B after 27 minutes.
Odmah nakon ubrizgavanja pokreće se linearni gradijent, posljedica čega je 5 % viši postotak od B nakon 27 minuta.
Subsequently a linear gradient is started, which brings the eluent composition to 90 % B in five minutes.
Potom se pokreće linearni gradijent koji u pet minuta mijenja sastav eluensa u 90 % B.
This composition is maintained for five minutes, after which the composition is changed, via a linear gradient in five minutes to the initial composition.
Taj se sastav održava pet minuta, nakon čega se pomoću linearnog gradijenta mijenja, da bi se nakon pet minuta ponovo vratio na početni sastav.
Depending on the internal volume of the pumping system, the next injection can be made 15 minutes after reaching the initial conditions.
Ovisno o unutarnjem volumenu pumpnog sustava, sljedeće se ubrizgavanje može izvršiti 15 minuta nakon postizanja početnih uvjeta.
This can be achieved by varying the initial and end conditions of the first gradient.
To je moguće postići mijenjanjem početnih i konačnih uvjeta prvog gradijenta.
However, the difference in the % B for the initial and end conditions of the first gradient shall remain 5 % B.
Međutim, razlika u % B između početnih i konačnih uvjeta prvog gradijenta mora ostati 5 % B.
This is essential for proper functioning of the gradient pumping system.
To je od ključne važnosti za pravilan rad gradijentnog pumpnog sustava.
The standard deviation for the retention time of the CMPA peak should be smaller than 0,1 minutes (n = 10).
Standardna devijacija vremena zadržavanja pika CMPA mora biti manja od 0,1 minute (n = 10).
The results of the chromatographic analysis of the test sample (E) are obtained in the form of a chromatogram in which the CMPA peak is identified by its retention time of about 26 minutes
Rezultati kromatografske analize ispitnog uzorka (E) dobiju se u obliku kromatograma u kojem se pik CMPA utvrđuje na temelju vremena zadržavanja koje iznosi oko 26 minuta.
The integrator (6.11.6) automatically calculates the peak height H of the CMPA peak.
Integrator (6.11.6.) automatski izračunava visinu H pika CMPA.
The baseline location should be checked in every chromatogram.
Na svakom kromatogramu treba provjeriti položaj bazne linije.
The analysis or the integration should be repeated if the baseline was incorrectly located.
Analizu ili integriranje treba ponoviti ako bazna linija nije pravilno smještena.
Note: If the CMPA peak is sufficiently separated from other peaks valley-to-valley baseline allocation should be used, otherwise use dropping perpendiculars to a common baseline, which should have starting point close to the CMPA peak (thus not at t = 0 min!).Use for the standard and the samples the same type integration type and check in case of common baseline its consistency for the samples and the standard.
Napomena: Ako je pik CMPA dovoljno odvojen od drugih pikova, treba koristiti alokaciju bazne linije dolina-dolina, u suprotnom slučaju koristite padajuće okomice na baznu liniju, koje trebaju imati ishodište blizu pika CMPA (prema tome ne kad je t = 0 min!). Za standard i uzorke primjenjujte istu vrstu integracije te u slučaju zajedničke bazne linije provjerite njezinu konzistentnost za uzorke i standard.
It is essential to examine the appearance of each chromatogram prior to quantitative interpretation, in order to detect any abnormalities due either to malfunctioning of the apparatus or the column, or to the origin and nature of the sample analysed.
Prije kvantitativne interpretacije od ključne je važnosti analizirati izgled svakog kromatograma radi otkrivanja bilo kakvih nepravilnosti nastalih zbog neispravnog rada uređaja ili kolona, ili zbog podrijetla i vrste analiziranog uzorka.
Apply exactly the procedure described from point 8.2 to point 8.4.4 to the standard samples (5.4.1 to 5.4.2).
Na standardne uzorke (od 5.4.1. do 5.4.2.) točno primijenite postupak opisan od točke 8.2. do točke 8.4.4.
Use freshly prepared solutions, because CMP degrades in an 8 % trichloroacetic acid environment at room temperature.
Upotrebljavajte svježe pripremljene otopine budući da se u 8-postotnoj triklorooctenoj kiselini na sobnoj temperaturi CMP razgrađuje.
At 4 °C the solution remains stable for 24 hours.
Na temperaturi od 4 °C otopina ostaje stabilna 24 sata.
In the case of long series of analyses the use of a cooled sample tray in the automatic injector is desirable
U slučaju velike serije analiza, poželjna je uporaba ohlađenog stalka za uzorke u automatskom injektoru.
Note: 8.4.2. may be omitted if the % B at initial conditions is known from previous analyses.
Napomena: točka 8.4.2. može se izostaviti ako je % B u početnim uvjetima poznat iz prethodnih analiza.
The chromatogram of the reference sample [5] should be analogous to Figure.
Kromatogram referentnog uzorka [5] mora biti analogan slici 1.
In this figure the CMPA peak is preceded by two small peaks.
Na toj slici piku CMPA prethode dva manja pika.
It is essential to obtain a similar separation.
Ključno je dobiti slično razdvajanje.
Prior to chromatographic determination of the samples inject 100 μl of the standard sample without rennet whey [0] (5.4.1)
Prije kromatografskog određivanja uzoraka ubrizgajte 100 μl standardnog uzorka bez slatke sirutke [0] (5.4.1.).
The chromatogram should not show a peak at the retention time of the CMPA peak.
Kromatogram ne smije pokazivati pik u vremenu zadržavanja pika CMPA.
Determine the response factors R by injecting the same volume of filtrate (8.5.1) as used for the samples.
Odredite faktore odziva R ubrizgavanjem količine filtrata (8.5.1.) jednake količini koja je korištena za uzorke.
Calculation of the response factor R:
Izračun faktora odziva R:
CMPA peak: R = W/H
Pik CMPA: R = W/H
R = the response factor of the CMPA peak
R = faktor odziva za pik CMPA;
H = the height of the CMPA peak
H = visina pika CMPA;
W = the quantity of whey in the standard sample [5].
W = količina sirutke u standardnom uzorku [5].
Calculation of the percentage of rennet whey powder in the sample
Izračun postotka slatke sirutke u prahu u uzorku
W(E) = the percentage (m/m) of rennet whey in the sample (E).
W(E) = postotak (m/m) slatke sirutke u uzorku (E);
R = the response factor of the CMPA peak (9.1.1)
R = faktor odziva za pik CMPA (9.1.1.);
H(E) = the height of the CMPA peak of the sample (E)
H(E) = visina pika CMPA uzorka (E).
If W(E) is greater than 1 % and the difference between the retention time and that of the standard sample [5] is smaller than 0,2 minutes then rennet whey solids are present.
Ako je W(E) veći od 1 % i ako je razlika između vremena zadržavanja i vremena standardnog uzorka [5] manja od 0,2 minute, to ukazuje na prisutnost suhe tvari slatke sirutke.
Not determined.
Nije određena.
From 0 to 16 % of rennet whey a linear relationship should be obtained with a coefficient of correlation > 0,99.
Od 0 do 16 % slatke sirutke linearni se odnos treba dobiti s korelacijskim koeficijentom > 0,99.
The 1 % limit includes the uncertainty due to reproducibility.
Granica od 1 % uključuje nesigurnost zbog obnovljivosti.
Ni—4.6 standard
Ni—4.6 standard vrijeme (u minutama)
Precision characteristics of analytical methods.
Obilježja preciznosti metoda analize.
Outline of collaborative study procedure.’
Prikaz postupka međulaboratorijske studije.”
the following Annexes are added:
Dodaju se sljedeći prilozi:
Methods of analysis of butter under private storage
Metode analize maslaca u privatnom skladištenju
Non Fat Solids (excluding salt)
Bezmasna suha tvar (bez soli)
Salt
Sol
Methods of analysis of skimmed milk powder under private storage
Metode analize obranog mlijeka u prahu u privatnom skladištenju
Methods of analysis of cheeses under private storage
Metode analize sira u privatnom skladištenju
The method of analysis laid down in the Appendix shall be used to ensure that cheese made exclusively from ewe's milk, goat's milk or buffalo milk or from a mixture of ewe's milk, goat's milk and buffalo milk does not contain cow's milk casein.
Metoda analize utvrđena u Dodatku upotrebljava se kako bi se osiguralo da sir napravljen isključivo od ovčjeg, kozjeg ili bivoljeg mlijeka ili od mješavine ovčjeg, kozjeg ili bivoljeg mlijeka ne sadržava kazein kravljeg mlijeka.
Cow's milk casein is considered to be present if the cow's milk casein content of the analysed sample is equal to or higher than the content of the reference sample containing 1 % cow's milk as laid down in the Appendix.
Ako je udio kazeina kravljeg mlijeka u analiziranom uzorku jednak udjelu u referentnom uzorku koji sadržava 1 % kravljeg mlijeka ili viši od njega, kako je utvrđeno u Dodatku, smatra se da je kazein kravljeg mlijeka prisutan.
Methods for detecting cow's milk casein in cheeses referred to in paragraph 1 may be used provided that:
Metode za detekciju kazeina kravljeg mlijeka u sirevima iz stavka 1. mogu se primijeniti pod uvjetom da:
the detection limit is maximum 0,5 % and
granica detekcije iznosi maksimalno 0,5 %;
there are no false-positive results and
nema lažno pozitivnih rezultata;
cow's milk casein is detectable with the required sensitivity even after long ripening periods, as may occur in usual commercial conditions.
kazein kravljeg mlijeka može se detektirati uz potrebnu osjetljivost čak i nakon dužih razdoblja zrenja, što se može dogoditi u uobičajenim trgovinskim uvjetima.
If any of the above mentioned requirements is not met, the methods laid down in the Appendix shall be used.
Ako bilo koji od gore navedenih zahtjeva nije ispunjen, upotrebljavaju se referentne metode utvrđene u Dodatku.
METHOD FOR THE DETECTION OF COW'S MILK AND CASEINATE IN CHEESES FROM EWE'S MILK, GOAT'S MILK OR BUFFALO MILK OR MIXTURES OF EWE'S MILK, GOAT'S MILK AND BUFFALO MILK
METODA ZA DETEKCIJU KRAVLJEG MLIJEKA I KAZEINATA U SIREVIMA OD OVČJEG, KOZJEG ILI BIVOLJEG MLIJEKA ILI OD MJEŠAVINA OVČJEG, KOZJEG I BIVOLJEG MLIJEKA