natgillin/translations-raw huggingface dataset:
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For the assays covered in this test method using cell lines, cytotoxicity is identified as a reduction in relative population doubling (RPD) or relative increase in cell count (RICC) of the treated cells as compared to the negative control (see paragraph 17 and Appendix 2).
za testove obuhvaćene ovom ispitnom metodom u kojima se upotrebljavaju stanične linije citotoksičnost je utvrđena kao smanjenje relativnog udvostručenja populacije (RPD) ili relativnog povećanja broja stanica (RICC) tretiranih stanica u odnosu na negativnu kontrolu (vidjeti stavak 17. i Dodatak 2.).
For the assays covered in this test method using primary cultures of lymphocytes, cytotoxicity is identified as a reduction in mitotic index (MI) of the treated cells as compared to the negative control (see paragraph 18 and Appendix 2).
Za testove obuhvaćene ovom ispitnom metodom u kojima se upotrebljavaju primarne kulture limfocita citotoksičnost je utvrđena kao smanjenje mitotskog indeksa tretiranih stanica u odnosu na negativnu kontrolu (vidjeti stavak 18. i Dodatak 2.).
for short-term studies: individual weight of the animals at the start and end of the test; for studies longer than one week: individual body weights during the study and food consumption.
za kratkotrajna istraživanja: pojedinačna tjelesna masa životinja na početku i na kraju ispitivanja; za istraživanja dulja od tjedan dana: pojedinačna tjelesna masa tijekom istraživanja i unos hrane.
Body weight range, mean and standard deviation for each group should be included.
Trebalo bi uključiti i raspon tjelesne mase te srednju izmjerenu vrijednost i standardno odstupanje za svaku skupinu.
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for short term studies: individual weight of the animals at the start and end of the test; for studies longer than one week: individual body weights during the study and food consumption.
za kratkotrajna istraživanja: pojedinačna tjelesna masa životinja na početku i na kraju ispitivanja; za istraživanja dulja od tjedan dana: pojedinačna tjelesna masa tijekom istraživanja i unos hrane.
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, A rapid in vivo test for chromosomal damage, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 18/2, str. 187. – 190.
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Environmental Protection Agency Gene-Tox Program, Mutation Research/Reviews in Genetic Toxicology, Vol. 123/1, pp. 61-118.
Environmental Protection Agency Gene-Tox Program, Mutation Research/Reviews in Genetic Toxicology, Vol. 123/1, str. 61. – 118.
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Environmental Protection Agency Gene-Tox Program, Mutation Research/Reviews in Genetic Toxicology, Vol. 239/1, str. 29. – 80.
CSGMT/JEMS.MMS — The Mammalian Mutagenesis Study Group of the Environmental Mutagen Society of Japan (1995), Protocol recommended by the CSGMT/JEMS.MMS for the short-term mouse peripheral blood micronucleus test.
CSGMT/JEMS.MMS – The Mammalian Mutagenesis Study Group of the Environmental Mutagen Society of Japan (1995), Protocol recommended by the CSGMT/JEMS.MMS for the short-term mouse peripheral blood micronucleus test.
The Collaborative Study Group for the Micronucleus Test (CSGMT) (CSGMT/JEMS.MMS, The Mammalian Mutagenesis Study Group of the Environmental Mutagen Society of Japan), Mutagenesis, Vol. 10/3, pp. 153-159.
The Collaborative Study Group for the Micronucleus Test (CSGMT) (CSGMT/JEMS.MMS, The Mammalian Mutagenesis Study Group of the Environmental Mutagen Society of Japan), Mutagenesis, Vol. 10/3, str. 153. – 159.
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Mavournin (1994), Bone marrow micronucleus assay: a review of the mouse stocks used and their published mean spontaneous micronucleus frequencies, Environmental and Molecular Mutagenesis, Vol. 23/4, str. 239. – 273.
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Galloway (1999), Rapid method for improving slide quality in the bone marrow micronucleus assay; an adapted cellulose column procedure, Mutation Research, Vol. 439/1, str. 121. – 126.
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The latter represents the protocol used for the European Community-sponsored prevalidation study conducted in 1997-1998.
Potonji protokol primjenjivao se u predvalidacijskom istraživanju koje je naručila Europska zajednica i koje je provedeno 1997. i 1998.
Test Method Evaluation Report — In Vitro Ocular Toxicity Test Methods for Identifying Ocular Severe Irritants and Corrosives.
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ICCVAM (2010).
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UN (2011).
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U: ICCVAM Test Method Evaluation Report – in vitro Ocular Toxicity Test Methods for Identifying Ocular Severe Irritants and Corrosives.
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For any laboratory initially establishing this assay, the proficiency chemicals provided in Appendix 2 should be used.
Svi laboratoriji koji su započeli s uspostavljanjem ove analize trebali bi upotrebljavati kemikalije za ispitivanje osposobljenosti navedene u Dodatku 2.
A laboratory can use these chemicals to demonstrate their technical competence in performing the ICE test method prior to submitting ICE data for regulatory hazard classification purposes.
Laboratorij može upotrebljavati te kemikalije kako bi dokazao svoju tehničku osposobljenost za provedbu ICE ispitne metode prije dostave podataka o ICE ispitnoj metodi u svrhu regulatornog razvrstavanja s obzirom na opasnost.
Interpretation of corneal swelling (Table 3), opacity (Table 4), and fluorescein retention (Table 5) using four ICE classes is done according to the scales shown below.
Oticanje rožnice (tablica 3.), zamućenje rožnice (tablica 4.) i zadržavanje fluoresceina (tablica 5.) tumače se primjenom četiriju ICE razreda u skladu s ljestvicama navedenima u nastavku.
It is important to note that the corneal swelling scores shown in Table 3 are only applicable if thickness is measured with a slit-lamp microscope (for example Haag-Streit BP900) with depth-measuring device no. 1 and slit-width setting at 91
Važno je napomenuti da su vrijednosti oticanja rožnice prikazane u tablici 3. primjenjive samo ako se debljinu mjeri mikroskopom (npr. procjepnom svjetiljkom Haag-Streit BP900) s uređajem za mjerenje dubine br. 1. i širinom procjepa podešenom na 9 1
0,0-0,5
0,0 – 0,5
0,6-1,5
0,6 – 1,5
1,6-2,5
1,6 – 2,5
2,6-4,0
2,6 – 4,0
2,6-3,0
2,6 – 3,0
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However, for thorough evaluation, which would be needed to conclude a negative outcome, all three following experimental conditions should be conducted using a short term treatment with and without metabolic activation and long term treatment without metabolic activation (see paragraphs 56, 57 and 58):
Međutim, za temeljitu procjenu, koja će biti potrebna za donošenje zaključka o negativnom ishodu, potrebno je primijeniti sva tri sljedeća pokusna uvjeta primjenom kratkotrajnog tretiranja s metaboličkom aktivacijom i bez nje te dugotrajnog tretiranja bez metaboličke aktivacije (vidjeti stavke 56., 57. i 58.):
Cells should be exposed to the test chemical without metabolic activation for 3-6 hours, and sampled at a time equivalent to about 1,5 - 2,0 normal cell cycle lengths after the beginning of treatment (19),
stanice je potrebno izlagati ispitivanoj kemikaliji bez metaboličke aktivacije od tri do šest sati te ih uzorkovati po isteku razdoblja koje otprilike odgovara trajanju od 1,5 do 2,0 normalna stanična ciklusa nakon početka tretiranja (19.),
Appropriate techniques should be used to control any bias or drift when using an automated scoring system, for instance, flow cytometry, laser scanning cytometry or image analysis.
Kad se primjenjuju automatski sustavi za brojenje, na primjer protočna citometrija, laserska skenirajuća citometrija ili slikovna analiza, trebalo bi primijeniti odgovarajuće tehnike za kontrolu mogućeg utjecaja ili pomaka.
Regardless of the automated platform is used to enumerate micronuclei, CBPI, RI, RPD, or RICC should be assessed concurrently.
Bez obzira na to koja se automatska platforma upotrebljava za brojenje mikronukleusa, potrebno je istodobno procijeniti CBPI, RI, RPD i RICC.
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