natgillin/translations-raw huggingface dataset:
The BCOP test method is also recommended to identify chemicals that do not require classification for eye irritation or serious eye damage, as defined by the UN GHS (UN GHS No Category) (4) within a testing strategy such as the Bottom-up approach (5). However, a chemical that is not predicted as causing serious eye damage or as not classified for eye irritation/serious eye damage with the BCOP test method would require additional testing (in vitro and/or in vivo) to establish a definitive classification.
BCOP ispitna metoda preporučuje se i za utvrđivanje kemikalija koje nije potrebno razvrstati s obzirom na nadraživanje oka ili tešku ozljedu oka, kako je utvrđeno sustavom UN GHS (bez kategorije prema sustavu UN GHS (4.) u okviru ispitne strategije kao što je pristup odozdo prema gore (5.) Međutim, za kemikaliju za koju nije predviđeno da će uzrokovati tešku ozljedu oka ili da će biti nerazvrstana s obzirom na nadraživanje oka / tešku ozljedu oka na temelju BCOP ispitne metode bit će potrebno dodatno ispitivanje (in vitro i/ili in vivo) za utvrđivanje konačnog razvrstavanja.
However, the MNvit test as described in this test method does not allow for the differentiation of chemicals inducing changes in chromosome number and/or ploidy from those inducing clastogenicity without special techniques such as FISH mentioned under paragraph 4. The MNvit test is robust and can be conducted in a variety of cell types, and in the presence or absence of cytoB.
Međutim, mikronukleus-testom in vitro opisanim u ovoj ispitnoj metodi ne omogućuje se razlikovanje kemikalija koje uzrokuju promjene u broju kromosoma i/ili ploidiju od onih koje uzrokuju klastogenost bez primjene posebnih tehnika, poput metode FISH navedene u stavku 4.
If it is known or suspected that the test chemical affects the cell cycling time (e.g. when testing nucleoside analogues), especially for p53 competent cells (35) (36) (77), sampling or recovery times may be extended by up to a further 1,5 - 2,0 normal cell cycle lengths (i.e. total 3,0 to 4,0 cell cycle lengths after the beginning of short-term and long-term treatments). These options address situations where there may be concern regarding possible interactions between the test chemical and cytoB.
Ako je poznato da ispitivana kemikalija utječe na duljinu staničnog ciklusa ili se na to sumnja (npr. pri ispitivanju analognih nukleozida), posebno za stanice kompetentne u pogledu p53 (35., 36., 77.), uzorkovanje ili obnavljanje može se produljiti za dodatno razdoblje koje odgovara trajanju od 1,5 do 2,0 normalna stanična ciklusa (tj. ukupno od 3,0 do 4,0 stanična ciklusa nakon početka kratkotrajnih i dugotrajnih tretiranja).
Cells containing more than two main nuclei should not be analysed for micronuclei, as the baseline micronucleus frequency may be higher in these cells (84). Scoring of mononucleate cells is acceptable if the test chemical is shown to interfere with cytoB activity. A repeat test without CytoB might be useful in such cases.
Stanice koje imaju više od dviju glavnih jezgri ne analiziraju se na mikronukleuse jer osnovna učestalost mikronukleusa u tim stanicama može biti veća (84.).
Brookmire L., J.J. Chen, D.D.
Brookmire L., J.J.
Levy (2013).
Chen, D.D. Levy (2013).
Reference control C: included in the analysis sequence to verify that the solvent used to dissolve the test chemical does not impact the percent peptide depletion. Calibration standards and reference controls STD1 STD2 STD3 STD4 STD5 STD6 Dilution buffer Reference control A, rep 1 Reference control A, rep 2 Reference control A, rep 3 Co-elution controls
referentna kontrola C: uključuje se u analitički ciklus kako bi se provjerilo da otapalo koje je upotrijebljeno za otapanje ispitivane kemikalije ne utječe na postotak potrošnje peptida.
Co-elution control 1 for test chemical 1 Co-elution control 2 for test chemical 2 Reference controls
Kalibracijski standardi i referentne kontrole
Reference control B, rep 1 Reference control B, rep 2 Reference control B, rep 3 First set of replicates
Standard 1.
Reference control C, rep 1 Cinnamic aldehyde, rep 1 Sample 1, rep 1 Sample 2, rep 1
Standard 2.
Second set of replicates Reference control C, rep 2 Cinnamic aldehyde, rep 2 Sample 1, rep 2 Sample 2, rep 2 Third set of replicates Reference control C, rep 3 Cinnamic aldehyde, rep 3 Sample 1, rep 3 Sample 2, rep 3 Reference controls
Standard 3.
NAFTA (North American Free Trade Agreement) (2012). Technical Working Group on Pesticides — (Quantitative) Structure Activity Relationship ((Q)SAR) Guidance Document.
NAFTA (North American Free Trade Agreement) (2012).
Scott, L. et al. (2010), A proposed eye irritation testing strategy to reduce and replace in vivo studies using Bottom-Up and Top-Down approaches, Toxicol.
Scott, L. et al. (2010), A proposed eye irritation testing strategy to reduce and replace in vivo studies using Bottom-Up and Top-Down approaches, Toxicol.
Proficiency Chemicals A sub-set of the list of Reference Chemicals that can be used by a naïve laboratory to demonstrate proficiency with the validated reference test method.
skupina kemikalija koje se nalaze na popisu referentnih kemikalija i koje laboratorij koji još nikada nije primijenio validiranu referentnu metodu može upotrijebiti kako bi dokazao tehničku osposobljenost za provođenje te metode.
General clinical observations related to the health of the animals should be made and recorded at least once a day preferably at the same time(s) each day and considering the peak period of anticipated effects after dosing (54). At least twice daily, all animals should be observed for morbidity and mortality.
Opća klinička opažanja zdravlja životinja trebalo bi provoditi i evidentirati najmanje jednom dnevno, po mogućnosti svaki dan u isto vrijeme, vodeći računa o razdoblju najvećeg intenziteta očekivanih učinaka nakon primjene doze (54.) Najmanje dva puta dnevno treba provjeravati ima li slučajeva pobolijevanja ili smrti životinja.
staining techniques used; and methods for scoring and measuring comets.
primijenjene tehnike bojenja i metode pregledavanja i mjerenja kometa.
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Kushwaha, S. et al. (2010), Evaluation of multi-organ DNA damage by Comet assay from 28 days repeated dose oral toxicity test in mice: A practical approach for test integration in regulatory toxicity testing, Regulatory Toxicology and Pharmacology, Vol. 58/1, str. 145.–54.
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Toyoizumi, T. et al. (2011), Use of the in vivo skin Comet assay to evaluate the DNA-damaging potential of chemicals applied to the skin, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 726/2, pp. 175-80.
Toyoizumi, T. et al. (2011), Use of the in vivo skin Comet assay to evaluate the DNA-damaging potential of chemicals applied to the skin, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 726/2, str. 175.–80.
Wada, K. et al. (2012), A comparison of cell-collecting methods for the Comet assay in urinary bladders of rats, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 742/1-2, pp. 26-30.
Wada, K. et al. (2012), A comparison of cell-collecting methods for the Comet assay in urinary bladders of rats, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 742/1-2, str. 26.–30.
Wang, A. et al. (2007), Measurement of DNA damage in rat urinary bladder transitional cells: improved selective harvest of transitional cells and detailed Comet assay protocols, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 634/ 1-2, pp. 51-9.
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Jackson, P. et al. (2012), Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring, Nanotoxicology, Vol. 6/5, pp. 486-500.
Jackson, P. et al. (2012), Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: effects on liver DNA strand breaks in dams and offspring, Nanotoxicology, Vol. 6/5, str. 486.–500.
Sekihashi, K. et al. (2002), Comparative investigations of multiple organs of mice and rats in the Comet assay, Mutation Research, Vol. 517/1-2, pp. 53-74.
Sekihashi, K. et al. (2002), Comparative investigations of multiple organs of mice and rats in the Comet assay, Mutation Research, Vol. 517/1-2, str. 53.–74.
Cordelli, E. et al. (2003), Evaluation of DNA damage in different stages of mouse spermatogenesis after testicular X irradiation, Journal of Radiation Research, Vol. 160/4, pp. 443-451.
Cordelli, E. et al. (2003), Evaluation of DNA damage in different stages of mouse spermatogenesis after testicular X irradiation, Journal of Radiation Research, Vol. 160/4, str. 443.–451.
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Kumaravel, T.S. et al. (2009), Comet Assay measurements: a perspective, Cell Biology and Toxicology, Vol. 25/1, str. 53.–64.
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Møller, P. et al. (2010), Assessment and reduction of Comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group, Mutagenesis, Vol. 25/2, str. 109.–11.
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Forchhammer, L. et al. (2010), Variation in the measurement of DNA damage by Comet assay measured by the ECVAG inter-laboratory validation trial, Mutagenesis, Vol. 25/2, str. 113.–23.
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Azqueta, A. et al. (2011), Towards a more reliable comet assay: Optimising agarose concentration, unwinding time and electrophoresis conditions, Mutation Research, Vol. 724/1-2, str. 41.–45.
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Hayashi, M. et al. (2011), Compilation and use of genetic toxicity historical control data, Mutation Research, Vol. 723/2, str. 87.–90.
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Bright, J. et al. (2011), Recommendations on the statistical analysis of the Comet assay, Pharmaceutical Statistics, Vol. 10/6, str. 485.–93.
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The duration can be lengthened if necessary (cf. paragraph 18), or shortened if it is demonstrated that steady-state has been reached earlier (see Appendix 1, definitions and units). A prediction of the length of the uptake phase and the time to steady-state can be made from equations in Appendix 5.
Trajanje se može produljiti prema potrebi (vidjeti stavak 18.) ili skratiti ako se dokaže da je stabilno stanje postignuto ranije (vidjeti Dodatak 1. o definicijama i jedinicama.) Trajanje faze apsorpcije i vrijeme potrebno da se postigne stabilno stanje može se predvidjeti uz pomoć jednadžbi iz Dodatka 5.
In such a case it is necessary that at least two, but preferably more, concentrations are tested (cf. paragraph 49) which are environmentally relevant. Also for substances where the concentrations tested have to be near the solubility limit for practical reasons, testing at least two concentrations is recommended, because this can give insight into the reliability of the exposure concentrations.
U tim je slučajevima potrebno ispitati najmanje dvije koncentracije koje su relevantne za okoliš, a ako je moguće i više njih (vidjeti stavak 49.) Za tvari kod kojih ispitivane koncentracije moraju iz praktičnih razloga biti blizu granice topljivosti isto se tako preporučuje ispitivanje s najmanje dvije koncentracije jer se tako može dobiti uvid u pouzdanost koncentracija pri izlaganju.
Alternatively, k1 and k2 can be determined sequentially (see Appendix 5 for a description and comparison of these methods). The depuration rate constant (k2) may need correction for growth dilution (cf. paragraphs 72 and 73).
Druga je mogućnost sekvencijalno utvrđivanje k1 i k2 (opis i usporedba tih metoda navedeni su u Dodatku 5.) Konstanta brzine izlučivanja (k2) možda će trebati biti ispravljena u odnosu na razrjeđenje zbog rasta (vidjeti stavke 72. i 73.).
Typically, the minimised test is not run over a shorter period than the standard BCF test, but comprises less fish sampling (see Appendix 6 for the rationale). However, the depuration period may be shortened for rapidly depurating substances to avoid concentrations in the fish falling below the limit of detection/quantification before the end of the test.
Uobičajeno trajanje skraćenog ispitivanja nije kraće od trajanja standardnog ispitivanja vrijednosti BCF-a, ali je u njega uključeno manje uzorkovanja riba (obrazloženje se nalazi u Dodatku 6.) Međutim, razdoblje izlučivanja može se skratiti kod tvari koje se brzo izlučuju kako bi se izbjeglo da koncentracije u ribama padnu ispod granice detekcije/kvantifikacije prije kraja ispitivanja.
3,0 ± 0,5
3,0 ± 0,5
5,0 ± 2,0
5,0 ± 2,0
8,0 ± 4,0 [3]
8,0 ± 4,0 [3]
(Teleostei, Poecilliidae)
(Teleostei, Poeciliidae)
4,0 ± 1,0
4,0 ± 1,0
3,0 ± 1,0
3,0 ± 1,0
8,0 ± 4,0
8,0 ± 4,0
The minimised BCFSS is the BCF calculated at the end of the uptake phase, assuming that steady-state has been reached. This can only be assumed, as the number of sampling points is not sufficient for proving this.
Vrijednost BCFSS iz skraćenog ispitivanja vrijednost je BCF-a izračunana na kraju faze apsorpcije, pod pretpostavkom da je postignuto stabilno stanje To je moguće samo pretpostaviti jer dokazivanje nije moguće zbog nedovoljnog broja točki uzorkovanja. BCFSS
t duration of the feeding period (day) However, the feeding rate, I, used in the calculation may need to be adjusted for fish growth to give an accurate assimilation efficiency, a. In a test where fish grow significantly during the uptake phase (in which no correction of feed quantities is made to maintain the set feeding rate), the effective feeding rate as the uptake phase progresses will be lower than that set, resulting in a higher 'real' assimilation efficiency.
t trajanje razdoblja hranjenja (u danima)
Any water in which the test species shows suitable long-term survival and growth may be used as test water. It should be of constant quality during the period of the test.
Kao ispitna voda može se koristiti svaka voda koja je prikladna za preživljavanje i rast ispitne vrste u dovoljno dugom razdoblju.
In setting the range of test concentrations, care should be taken not to exceed the maximum tolerated concentration to allow a meaningful interpretation of the data. It is important to have at least one treatment where there are no signs of toxic effects.
Pri određivanju raspona ispitnih koncentracija potrebno je paziti da se ne premaši maksimalna tolerirana koncentracija kako bi se moglo valjano tumačiti podatke, Važno je da postoji najmanje jedna tretirana skupina u kojoj se ne pojavljuju znakovi toksičnih učinaka.
In the first case the test must be repeated. In the second one the loss of an entire treatment group(s) due to internal control mortality may limit the ability to evaluate effects and determine LC50 values.
U drugom slučaju gubitak cijele tretirane skupine / tretiranih skupina zbog smrtnosti u unutarnjoj kontroli može za posljedicu imati smanjivanje mogućnosti procjene učinaka i određivanja vrijednosti LC50.
Preparation of this media is described in the Appendix 1. The pH of the media (water phase) at test initiation should be between 7,5 and 8,0 for optimum plant growth.
Sastav tog medija naveden je u Dodatku 1. pH-vrijednost medija (vodena faza) na početku ispitivanja treba biti između 7,5 i 8,0 za optimalni rast biljaka.
Shoot fresh and dry weight, Visual assessment of roots
Svježa i suha masa izdanka, vizualna procjena korijenja
Arts, G.H.P. et al. (2008), Sensitivity of submersed freshwater macrophytes and endpoints in laboratory toxicity tests, Environmental Pollution, Vol. 153, pp. 199-206.
Arts, G.H.P. et al. (2008), Sensitivity of submersed freshwater macrophytes and endpoints in laboratory toxicity tests, Environmental Pollution, Vol. 153, str. 199-206.
Knauer, K. et al. (2006), Methods for assessing the toxicity of herbicides to submersed aquatic plants, Pest Management Science, Vol. 62/8, pp. 715-722.
Knauer, K. et al. (2006), Methods for assessing the toxicity of herbicides to submersed aquatic plants, Pest Management Science, Vol. 62/8, str. 715-722.
Norberg-King, T.J. (1988), An interpolation estimate for chronic toxicity: The ICp approach, National Effluent Toxicity Assessment Center Technical Report 05-88.
Norberg-King, T.J. (1988), An interpolation estimate for chronic toxicity: The ICp approach, National Effluent Toxicity Assessment Center Technical Report 05-88.
Commission Implementing Regulation (EU) 2017/736
Provedbena uredba Komisije (EU) 2017/736
amending Annex VIII to Regulation (EC) No 999/2001 of the European Parliament and of the Council as regards the approval of Slovenia's national control programme for classical scrapie
o izmjeni Priloga VIII. Uredbi (EZ) br. 999/2001 Europskog parlamenta i Vijeća u pogledu odobrenja slovenskog nacionalnog programa kontrole klasičnoga grebeža
Having regard to Regulation (EC) No 999/2001 of the European Parliament and of the Council of 22 May 2001 laying down rules for the prevention, control and eradication of certain transmissible spongiform encephalopathies [1], and in particular point 3.1(b) of Section A of Chapter A of Annex VIII thereof,
uzimajući u obzir Uredbu (EZ) br. 999/2001 Europskog parlamenta i Vijeća od 22. svibnja 2001. o utvrđivanju pravila za sprečavanje, kontrolu i iskorjenjivanje određenih transmisivnih spongiformnih encefalopatija [1], a osobito njezinu točku 3.1. podtočku (b) odjeljka A poglavlja A Priloga VIII.,
Regulation (EC) No 999/2001 lays down rules for the prevention, control and eradication of transmissible spongiform encephalopathies (TSEs) in bovine, ovine and caprine animals.
Uredbom (EZ) br. 999/2001 utvrđuju se pravila za sprečavanje, kontrolu i iskorjenjivanje transmisivnih spongiformnih encefalopatija (TSE) u goveda, ovaca i koza.
Article 15 of Regulation (EC) No 999/2001 provides that the placing on the market or, if need be, the export of bovine, ovine or caprine animals and their semen, embryos and ova are subject to the conditions laid down in Annex VIII thereto.
Člankom 15. Uredbe (EZ) br. 999/2001 predviđeno je da stavljanje na tržište, ili u slučaju potrebe, izvoz goveda, ovaca ili koza te njihova sjemena, zametaka i jajnih stanica podliježe uvjetima utvrđenima Prilogom VIII. toj Uredbi.
Point 3.1(a) of Section A of Chapter A of that Annex provides that a Member State which has a national control programme for classical scrapie (‘national programme’) covering all of its territory may submit its national programme to the Commission for approval.
Točkom 3.1. podtočkom (a) odjeljka A poglavlja A tog Priloga predviđeno je da država članica koja ima nacionalni program kontrole klasičnoga grebeža („nacionalni program”) na cijelom svojem državnom području može svoj nacionalni program dostaviti Komisiji radi odobrenja.
Point 3.1(b) of that Section provides that the Commission may approve that national programme if it complies with the criteria laid down in point 3.1(a) of that Section.
U točki 3.1. podtočki (b) tog odjeljka predviđeno je da Komisija može odobriti taj nacionalni program ako ispunjava kriterije utvrđene u točki 3.1. podtočki (a) tog odjeljka.
Point 3.2 of that Section lists the Member States whose national programmes have been approved.
U točki 3.2. tog odjeljka navode se države članice čiji su nacionalni programi odobreni.
On 13 September 2016, Slovenia submitted to the Commission a request for approval of its national programme.
Slovenija je 13. rujna 2016. Komisiji dostavila zahtjev za odobrenje svojeg nacionalnog programa.
On 8 January 2017, following requests for further information, Slovenia submitted an amended national programme, providing clarification and further information on certain aspects thereof.
Nakon zahtjeva za dodatne informacije, Slovenija je 8. siječnja 2017. dostavila izmijenjeni nacionalni program te pojašnjenje i dodatne informacije o njegovim određenim aspektima.
That amended national programme should be approved, as it complies with the criteria laid down in point 3.1(a) of Section A of Chapter A of Annex VIII to Regulation (EC) No 999/2001.
Taj bi izmijenjeni nacionalni program trebalo odobriti jer ispunjava kriterije utvrđene u točki 3.1. podtočki (a) odjeljka A poglavlja A Priloga VIII. Uredbi (EZ) br. 999/2001.