natgillin/translations-raw huggingface dataset:
Panter G.H., et al.
Panter G.H., et al. (2004).
Parks L.G., et al.
Parks L.G., et al. (1999).
Fenske M., et al.
Fenske M., et al. (2001).
Holbech H., et al.
Holbech H., et al. (2001).
Vitellogenin induction by 17β-estradiol and 17α-ethinylestradiol in male zebrafish (Danio rerio).
Vitellogenin induction by 17b-estradiol and 17a-ethinylestradiol in male zebrafish (Danio rerio).
C 131: 531-539.
C. 131: 531-539.
Brion F., et al.
Brion F., et al. (2002).
Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio).
Development and validation of an enzyme-linked immunosorbent assay to measure vitellogenin in the zebrafish (Danio rerio).
Yokota H., et al.
Yokota H., et al. (2001).
Development of an ELISA for determination of the hepatic vitellogenin in Medaka (Oryzias latipes).
Development of an ELISA for determination of the hepatic vitellogenin in Medaka (Oryzias latipes).
Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnow.
Effects of the androgenic growth promoter 17-beta-trenbolone on fecundity and reproductive endocrinology of the fathead minnow.
OECD (2010).
OECD (2010). Guidance Document on Fish Gonadal Histopathology.
Guidance Document on Fish Gonadal Histopathology.
OECD Environmental Health and Safety Publications Series on Testing and Assessment No.
OECD Environmental Health and Safety Publications Series on Testing and Assessment No. 123, Paris.
123, Pariz.
OECD (2000) Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures.
OECD (2000) Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures. Series on Testing and Assessment No.
Aquatic Toxicology, 76; pp.69–92.
Aquatic Toxicology, 76; pp. 69–92.
Miles-Richardson S.R., et al.
Miles-Richardson S.R., et al. (1999).
Martinovic D., et al.
Martinovic D., et al. (2008).
OECD (2012), OECD Conceptual Framework for Testing and Assessment of Endocrine Disrupters, OECD Environmental Health and Safety Publications, Series on Testing and Assessment No. 150, OECD, Paris.
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ABBREVIATIONS & DEFINITIONS:
KRATICE I DEFINICIJE
a substance or a mixture
tvar ili smjesa.
coefficient of variation
koeficijent varijacije
Enzyme-Linked Immunosorbent Assay
imunoenzimski test (eng. Enzyme-Linked Immunosorbent Assay).
hypothalamic-pituitary-gonadal axis
os hipotalamus-hipofiza-gonade.
Loading rate
Gustoća punjenja:
the wet weight of fish per volume of water.
mokra masa ribe po volumenu vode.
Maximum Tolerated Concentration, representing about 10 % of the LC50
maksimalna tolerirana koncentracija, iznosi oko 10 % vrijednosti LC50.
Stocking density
Gustoća nasada
is the number of fish per volume of water.
broj riba po volumenu vode.
vitellogenin is a phospholipoglycoprotein precursor to egg yolk protein that normally occurs in sexually active females of all oviparous species.
vitelogenin je fosfolipoglikoproteinski prekursor proteina žumanjka koji je u pravilu prisutan u spolno aktivnih ženki svih vrsta koje liježu jaja.
EXPERIMENTAL CONDITIONS FOR THE FISH ENDOCRINE SCREENING ASSAY
EKSPERIMENTALNI UVJETI ZA TEST PROBIRA ENDOKRINOG SUSTAVA RIBA
Flow-through
Protočno
Fluorescent bulbs (wide spectrum)
Fluorescentne žarulje (širokog spektra)
Light intensity
Jakost svjetla
10-20 μE/m2/s, 540-1000 lux, or 50-100 ft-c (ambient laboratory levels)
10 – 20 μE/m2/s, 540 – 1000 lux, ili 50 – 100 ft-c (razine u laboratorijskim uvjetima)
1,5 l (minimum)
Najmanje 1,5 l
Volume exchanges of test solutions
Izmjene volumena ispitnih otopina
Minimum of 5 daily
Najmanje pet puta dnevno
Age of test organisms
Starost ispitnih organizama
See paragraph 21
Vidjeti stavak 21.
Approximate wet weight of adult fish (g)
Približna mokra masa odraslih riba (g)
Females: 1,5 ± 20 %
Ženke: 1,5 ± 20 %
Males: 2,5 ± 20 %
Mužjaci: 2,5 ± 20 %
Females: 0,35 ± 20 %
Ženke: 0,35 ± 20 %
Males: 0,35 ± 20 %
Mužjaci: 0,35 ± 20 %
Females: 0,65 ± 20 %
Ženke: 0,65 ± 20 %
Males: 0,4 ± 20 %
Mužjaci: 0,4 ± 20 %
6 (3 males and 3 females)
Šest (tri mužjaka i tri ženke)
No. of treatments
Broj tretiranja
= 3 (plus appropriate controls)
= tri (uvećano za odgovarajući broj kontrola)
No. vessels per treatment
Broj posuda po tretiranju
16 adult females and 8 males (4 females and 2 males in each replicate vessel)
16 odraslih ženki i osam mužjaka (četiri ženke i dva mužjaka u svakoj posudi s ponovljenim uzorkom)
12 adult females and 12 males (3 females and 3 males in each replicate vessel)
12 odraslih ženki i 12 mužjaka (tri ženke i tri mužjaka u svakoj posudi s ponovljenim uzorkom)
10 adult females and 10 males (5 females and 5 males in each replicate vessel)
Deset odraslih ženki i deset mužjaka (pet ženki i pet mužjaka u svakoj posudi s ponovljenim uzorkom)
Feeding regime
Hranjenje
Live or frozen adult or nauplii brine shrimp two or three times daily (ad libitum), commercially available food or a combination of the above
Živi ili zamrznuti račići Artemia ili njihove ličinke dva ili tri puta dnevno (ad libitum), komercijalno dostupna hrana ili kombinacija prethodno navedenoga
Brine shrimp nauplii two or three times daily (ad libitum), commercially available food or a combination of the above
Ličinke račića Artemia dva ili tri puta dnevno (ad libitum), komercijalno dostupna hrana ili kombinacija prethodno navedenoga
Brine shrimp nauplii two or three times daily (ad libitum),commercially available food or a combination of the above
Ličinke račića Artemia dva ili tri puta dnevno (ad libitum), komercijalno dostupna hrana ili kombinacija prethodno navedenoga
None unless DO concentration falls below 60 % air saturation
Nikakvo, osim ako koncentracija otopljenog kisika padne ispod 60 % zasićenja zrakom
Clean surface, well or reconstituted water or dechlorinated tap water
Čista površinska, izvorska ili rekonstituirana voda ili voda iz slavine iz koje je uklonjen klor
Pre- exposure period
Razdoblje prije izlaganja
7-14 days recommended
Preporučuje se 7 – 14 dana
Biological endpoints
Biološke krajnje točke
fecundity
plodnost,
sex characteristics
spolna obilježja,
optionally gonadal histopathology
histopatološka pretraga gonada (nije obvezno)
Dissolved oxygen ≥ 60 % of saturation; mean temperature of 25 ± 2 °C; 90 % survival of fish in the controls; measured test concentrations within 20 % of mean measured values per treatment level.
Otopljeni kisik ≥ 60 % zasićenosti; srednja temperatura od 25 ± 2 °C; 90 % preživljavanja riba u kontrolnim skupinama; izmjerene ispitne koncentracije unutar 20 % srednjih izmjerenih vrijednosti za skupinu tretiranu određenom koncentracijom.
Dissolved oxygen ≥ 60 % of saturation; mean temperature of 26 ± 2 °C; 90 % survival of fish in the controls; measured test concentrations within 20 % of mean measured values per treatment level.
Otopljeni kisik ≥ 60 % zasićenosti; srednja temperatura od 26 ± 2 °C; 90 % preživljavanja riba u kontrolnim skupinama; izmjerene ispitne koncentracije unutar 20 % srednjih izmjerenih vrijednosti za skupinu tretiranu određenom koncentracijom.
SOME CHEMICAL CHARACTERISTICS OF ACCEPTABLE DILUTION WATER
NEKA KEMIJSKA SVOJSTVA PRIHVATLJIVE VODE ZA RAZRJEĐIVANJE
COMPONENT
KOMPONENTA
< 20 mg/l
< 20mg/l
Unionised ammonia
neionizirani amonijak
Total organophosphorus pesticides
ukupni organofosforni pesticidi
Appendix 4A
Dodatak 4.A
SPAWNING SUBSTRATE FOR ZEBRAFISH
SUPSTRAT ZA MRIJEŠĆENJE ZEBRICE
Spawning tray
Ladica za mrijest
all glass instrument dish, for example 22 × 15 × 5,5 cm (l × w × d), covered with a removable stainless steel wire lattice (mesh width 2mm).
staklena, dimenzija primjerice 22 × 15 × 5,5 cm (d × š × v), prekrivena pomičnom žičanom rešetkom od nehrđajućeg čelika (širina otvora 2 mm).
The lattice should cover the opening of the instrument dish at a level below the brim.
Rešetka bi trebala prekrivati otvor posude ispod ruba.
On the lattice, spawning substrate should be fixed.
Supstrat za mriješćenje treba pričvrstiti na rešetku.
It should provide structure for the fish to move into.
To bi trebao biti prostor u koji ribe mogu ulaziti.
For example, artificial aquaria plants made of green plastic material are suitable (NB: possible adsorption of the test chemical to the plastic material should be considered).
Primjerice, prikladno je umjetno akvarijsko bilje izrađeno od zelene plastike (napomena: potrebno je uzeti u obzir moguću adsorpciju ispitivane kemikalije na plastiku).
The plastic material should be leached out in sufficient volume of warm water for sufficient time to ensure that no chemicals may be disposed to the test water.
Plastiku je potrebno dovoljno dugo ispirati dovoljnom količinom tople vode kako bi se osiguralo da ne dođe do otpuštanja kemikalija u ispitnu vodu.
When using glass materials it should be ensured that the fish are neither injured nor cramped during their vigorous actions.
Pri upotrebi stakla potrebno je osigurati da ne dođe do ozljeđivanja ili prikliještenja riba tijekom njihova energičnoga kretanja.
The distance between the tray and the glass panes should be at least 3 cm to ensure that the spawning is not performed outside the tray.
Udaljenost između ladice i stakla trebala bi biti najmanje 3 cm kako bi se osiguralo da do mriješćenja ne dođe izvan ladice.
The eggs spawned onto the tray fall through the lattice and can be sampled 45-60 min after the start of illumination.
Jajašca izmriještena u ladicu padaju kroz rešetku i uzorci se mogu uzeti od 45 do 60 minuta nakon početka osvjetljavanja.
The transparent eggs are non-adhesive and can easily be counted by using transversal light.
Prozirna se jajašca ne lijepe i mogu se jednostavno prebrojiti s pomoću transverzalnog svjetla.
When using five females per vessel, egg numbers up to 20 at a day can be regarded as low, up to 100 as medium and more than 100 as high numbers.
Pri upotrebi pet ženki po posudi, do 20 jajašaca dnevno može se smatrati malim brojem, do 100 srednjim, a više od 100 velikim.
The spawning tray should be removed, the eggs collected and the spawning tray re-introduced in the test vessel, either as late as possible in the evening or very early in the morning.
Ladicu za mrijest potrebno je maknuti, jajašca ukloniti i vratiti ladicu u ispitnu posudu što kasnije navečer ili vrlo rano ujutro.
The time until re-introduction should not exceed one hour since otherwise the cue of the spawning substrate may induce individual mating and spawning at an unusual time.
Razdoblje do vraćanja ladice ne smije trajati dulje od jednog sata jer u suprotnom supstrat za mriješćenje može potaknuti pojedinačno parenje i mriješćenje u neuobičajeno doba.
If a situation needs a later introduction of the spawning tray, this should be done at least 9 hours after start of the illumination.
U slučaju potrebe za kasnijim vraćanjem ladice za mrijest to treba učiniti najmanje devet sati nakon početka osvjetljavanja.
At this late time of the day, spawning is not induced any longer.
U to kasno doba dana više nema mriješćenja.
Appendix 4B
Dodatak 4.B
SPAWNING SUBSTRATE FOR FATHEAD MINNOW
SUPSTRAT ZA MRIJEŠĆENJE DEBELOGLAVE GAVČICE
Two or three combined plastic/ceramic/glass or stainless steel spawning tiles and trays are placed in each of the test chamber (e.g. 80 mm length of grey semi-circular guttering sitting on a lipped tray of 130mm length) (see picture).
Dvije ili tri kombinirane pločice i ladice za mrijest od plastike/keramike/stakla ili nehrđajućeg čelika smještaju se u svaku ispitnu komoru (primjerice sivi polukružni oluk dužine 80 mm koji se nalazi na ladici U-profila dugačkoj 130 mm) (vidjeti sliku).
Properly seasoned PVC or ceramic tiles have demonstrated to be appropriate for a spawning substrate (Thorpe et al, 2007).
Pravilno pripremljene plastične ili keramičke pločice pokazale su se prikladnima za supstrat za mriješćenje (Thorpe et al. 2007).
The tray should also be screened to prevent fish from access to the fallen eggs unless the egg adhesion efficiency has been demonstrated for the spawning substrate used.
Ladicu treba pregledati kako bi se ribama spriječio pristup palim jajašcima, osim ako je prianjanje jajašaca dokazano za primijenjeni supstrat za mriješćenje.
All spawning substrates should be leached for a minimum of 12 hours, in dilution water, before use.
Sve supstrate za mriješćenje prije upotrebe potrebno je ispirati u vodi za razrjeđivanje najmanje 12 sati.
ASSESSMENT OF SECONDARY SEX CHARACTERISTICS IN FATHEAD MINNOW FOR THE DETECTION OF CERTAIN ENDOCRINE ACTIVE CHEMICALS
PROCJENA SEKUNDARNIH SPOLNIH OBILJEŽJA U DEBELOGLAVE GAVČICE RADI OTKRIVANJA ODREĐENIH ENDOKRINO AKTIVNIH KEMIKALIJA
Potentially important characteristics of physical appearance in adult fathead minnows in endocrine disrupter testing include body colour (i.e., light/dark), coloration patterns (i.e., presence or absence of vertical bands), body shape (i.e., shape of head and pectoral region, distension of abdomen), and specialised secondary sex characteristics (i.e., number and size of nuptial tubercles, size of dorsal pad and ovipositor).
Potencijalno važna obilježja fizičkog izgleda u odraslih debeloglavih gavčica pri ispitivanju endokrinih disruptora uključuju boju tijela (svijetlu/tamnu boju), uzorke boje (postojanje ili izostanak okomitih pruga), oblik tijela (oblik glave i prsnog područja, zaobljenost abdomena) te posebna sekundarna spolna obilježja (broj i veličinu izraslina spolno zrelih riba, veličinu zadebljanja ispred leđne peraje i leglice).
Nuptial tubercles are located on the head (dorsal pad) of reproductively-active male fathead minnows, and are usually arranged in a bilaterally-symmetric pattern (Jensen et al. 2001).
Izrasline se nalaze na glavi (zadebljanju ispred leđne peraje) reproduktivno aktivnih mužjaka debeloglave gavčice te su u pravilu raspoređene u bočno simetričnom uzorku (Jensen et al. 2001).
Control females and juvenile males and females exhibit no tubercle development (Jensen et al. 2001).
U ženki iz kontrolnih skupina i mladih mužjaka i ženki nije uočen razvoj izraslina (Jensen et al. 2001).
In many fish there are groups of tubercles below the lower jaw; those closest to the mouth generally occur as a single pair, while the more ventral set can be comprised of up to four tubercles.
U brojnih riba skupine izraslina nalaze se ispod donje čeljusti; jedan par izraslina u pravilu se nalazi najbliže ustima, dok se dalje prema trbuhu mogu nalaziti do četiri izrasline.
The actual numbers of tubercles is seldom more than 30 (range, 18-28; Jensen et al. 2001).
Stvarni broj izraslina rijetko premašuje 30 (raspon od 18 do 28; Jensen et al. 2001).
Some types of endocrine-disrupting chemicals can cause the abnormal occurrence of certain secondary sex characteristics in the opposite sex; for example, androgen receptor agonists, such as 17α-methyltestosterone or 17β-trenbolone, can cause female fathead minnows to develop nuptial tubercles (Smith 1974; Ankley et al. 2001; 2003), while oestrogen receptor agonists may decrease number or size of nuptial tubercles in males (Miles-Richardson et al. 1999; Harries et al. 2000).
Neke vrste endokrino disruptivnih kemikalija mogu prouzročiti neuobičajenu pojavu određenih sekundarnih spolnih obilježja u suprotnog spola; primjerice, agonisti receptora za androgene, poput 17α-metiltestosterona ili 17β-trenbolona, mogu prouzročiti razvoj izraslina u ženki debeloglave gavčice (Smith 1974; Ankley et al. 2001; 2003.), dok agonisti receptora za estrogene mogu prouzročiti smanjenje broja ili veličine izraslina u mužjaka (Miles-Richardson et al. 1999; Harries et al. 2000).
Below is a description of the characterisation of nuptial tubercles in fathead minnows based on procedures used at the U.S. Environmental Protection Agency lab in Duluth, MN.
U nastavku se nalazi opis obilježja izraslina u spolno zrelih debeloglavih gavčica na temelju postupaka koji se primjenjuju u laboratoriju američke Agencije za zaštitu okoliša u Duluthu, Minnesota.
Place fish in small Petri dish (e.g. 100 mm in diameter), anterior forward, and ventral down.
Smjestite ribe u malu Petrijevu zdjelicu (primjerice promjera 100 mm) glavom prema naprijed i trbuhom prema dolje.
A template was developed to map the location and quantity of tubercles present (see end of this appendix).
Izrađen je obrazac za evidentiranje mjesta i broja izraslina (vidjeti završetak ovog Dodatka).
The number of tubercles is recorded and their size can be quantitatively ranked as: 0- absence, 1-present, 2-enlarged and 3-pronounced for each organism (Fig. 1).
Broj se izraslina evidentira, a njihova se veličina ocjenjuje kao: 0 – izostanak, 1 – prisutne, 2 – uvećane i 3 – izražene za svaki organizam (slika 1.).
Rate 0 — absence of any tubercle.
Ocjena 0 – izostanak izraslina.
Rating 1 — present, is identified as any tubercle having a single point whose height is nearly equivalent to its radius.
Ocjena ‚1 – prisutne’ odnosi se na sve izrasline s jednostrukim vrhom čija je visina gotovo jednaka polumjeru.
Rating 2 — enlarged, is identified by tissue resembling an asterisk in appearance, usually having a large radial base with grooves or furrows emerging from the centre.
Ocjena ‚2 – uvećane’ odnosi se na tkivo koje nalikuje zvjezdici i koje u pravilu ima veliku radijalnu osnovu s udubinama i brazdama koje se izdižu iz središta.
Rating 3 — pronounced, is usually quite large and rounded with less definition in structure.
Ocjena ‚3 – izražene’ odnosi se na prilično velike i zaobljene izrasline slabije definirane strukture.
Using this rating system generally will result in overall tubercle scores of < 50 in a normal control male possessing a tubercle count of 18 to 20 (Jensen et al. 2001).
Posljedica će primjene ovog sustava ocjenjivanja biti ukupne ocjene izraslina < 50 u normalnog kontrolnog mužjaka koji ima od 18 do 20 izraslina (Jensen et al. 2001).
The actual number of tubercles in some fish may be greater than the template boxes for a particular rating area.
Stvarni broj izraslina u nekih riba može premašivati broj polja u obrascu za određeno područje ocjenjivanja.
A — Tubercles located around eye.
A – izrasline oko očiju.
Commonly multiple in mature control males, not present in control females, generally paired (one near each eye) or single in females exposed to androgens.
Često višestruke u zrelih mužjaka iz kontrolnih skupina, nisu prisutne u ženki iz kontrolnih skupina, u pravilu u paru (po jedna blizu svakog oka) ili pojedinačne u ženki izloženih androgenima.
B — Tubercles located between nares, (sensory canal pores).
B-izrasline između nosnica (pora osjetilnih kanala).
Normally in pairs for control males at more elevated levels (2- enlarged or 3- pronounced) of development.
U pravilu u parovima u mužjaka iz kontrolnih skupina na višim stadijima (2 – uvećane ili 3 – izražene) razvoja.
C — Tubercles located immediately anterior to nares, parallel to mouth.
C – izrasline neposredno ispred nosnica, paralelno s ustima.
D — Tubercles located parallel along mouth line.
D – izrasline smještene paralelno duž linije ustiju.
E — Tubercles located on lower jaw, close to mouth, usually small and commonly in pairs.
E – izrasline na donjoj čeljusti, blizu usta, u pravilu malene i obično u parovima.
F — Tubercles located ventral to E. Commonly small and paired.
F – izrasline s trbušne strane u odnosu na E. Obično malene i u parovima.
Tubercle Template:
Obrazac za ocjenu izraslina:
Total Score
Ukupan broj bodova
ASSESSMENT OF SECONDARY SEX CHARACTERISTICS IN MEDAKA FOR THE DETECTION OF CERTAIN ENDOCRINE ACTIVE CHEMICALS
PROCJENA SEKUNDARNIH SPOLNIH OBILJEŽJA U MEDAKE RADI OTKRIVANJA ODREĐENIH ENDOKRINO AKTIVNIH KEMIKALIJA
Below is a description of the measurement of papillary processes [1], which are the secondary sex characteristics in medaka (Oryzias latipes).
U nastavku se opisuje mjerenje papilarnih procesa [1] koji su sekundarno spolno obilježje u medake (Oryzias latipes).
After the excision of the liver (Appendix 6), the carcass is placed into a conical tube containing about 10 ml of 10 % neutral buffered formalin (upside: head, downside: tail).
Nakon vađenja jetre (Dodatak 6.) truplo se stavlja u epruvetu s približno 10 ml 10-postotnog neutralnog puferiranog formalina (glavom prema gore i repom prema dolje).
If the gonad is fixed in a solution other than 10 % neutral buffered formalin, make a transverse cut across the carcass between anterior region of anal fin and anus using razor, taking care not to harm the gonopore and gonad itself (Fig.3).
Ako je gonada fiksirana u otopini koja nije 10-postotni neutralni puferirani formalin, s pomoću skalpela napravite poprečni rez preko trupla između prednjeg dijela podrepne peraje i anusa, pazeći pritom da ne oštetite gonoporu i samu gonadu (slika 3.).
Place the cranial side of the fish body into the fixative solution to preserve the gonad, and the tail side of the fish body into the 10 % neutral buffered formalin as described above.
Stavite glavu s prednjim dijelom ribe u otopinu za fiksiranje radi očuvanja gonade, a repni dio u 10-postotni neutralni puferirani formalin prema prethodnom opisu.
After placing the fish body into 10 % neutral buffered formalin, grasp the anterior region of the anal fin with tweezers and fold it for about 30 seconds to keep the anal fin open.
Nakon što ste ribu stavili u 10-postotni neutralni puferirani formalin, pincetom uhvatite prednji dio podrepne peraje i držite ga presavijenog oko 30 sekundi kako biste raširili podrepnu peraju.
After keeping the anal fin open for about 30 seconds, store the fish body in 10 % neutral buffered formalin at room temperature until the measurement of the papillary processes (measurement should be conducted after fixing for at least 24 hours).
Nakon što je podrepna peraja bila raširena 30 sekundi, pohranite tijelo ribe u 10-postotni neutralni puferirani formalin na sobnoj temperaturi do mjerenja papilarnih procesa (mjerenje je potrebno provesti nakon najmanje 24-satnog fiksiranja).
Count the number of the joint plate with papillary processes using the counter under a biological microscope (upright microscope or inverted microscope).
Prebrojite kosti bodlje s papilarnim procesima s pomoću brojača pod biološkim mikroskopom (uspravni ili invertni mikroskop).
The papillary processes are recognised when a small formation of processes is visible on the posterior margin of joint plate.
Papilarni procesi uočavaju se kad je mala nakupina procesa vidljiva na stražnjem dijelu kosti bodlje.
Write the number of joint plate with papillary processes in each fin ray to the worksheet (e.g. first fin ray: 0, second fin ray: 10, third fin ray: 12, etc.) and enter the sum of this number on the Excel spreadsheet by individual fish.
U obrazac zabilježite broj kosti bodlje s papilarnim procesima na svakoj šipčici peraje (primjerice, prva šipčica peraje: 0, druga šipčica peraje: 10, treća šipčica peraje: 12 itd.) te u tablicu u Excelu unesite zbroj tih brojeva prema pojedinačnim ribama.
After the measurement, put the anal fin into the conical tube described in (1) and store it.
Nakon mjerenja stavite podrepnu peraju u epruvetu kako je opisano u točki 1. i pohranite je.
A, male; B, female.
A – mužjak; B – ženka.
Fig.2.
Slika 2.A
A, Processes on joint plates of anal fin-ray.
procesi na kostima bodlje šipčice podrepne peraje. J.P.
J.P., joint plate; A.S., axial space; P., process.
kost bodlje; A.S. – aksijalni prostor; P. – proces.
Photograph of fish body showing the cut site when the gonad is fixed in the fixing solution other than 10 % neutral buffered formalin.
Fotografija tijela ribe i prikaz mjesta reza kad je gonada fiksirana u otopini za fiksiranje koja nije 10-postotni neutralni puferirani formalin.
In that case, the remaining body will be cut off between anterior region of anal fin and anal using razor (red bar), and the head side of fish body will be put into the fixing solution for gonad and the tail side of the fish body will be put into the 10 % neutral buffered formalin.
U tom slučaju ostatak tijela odrezat će se između prednjeg dijela podrepne peraje i anusa s pomoću skalpela (crvena linija); glava s prednjim dijelom ribe stavit će se u otopinu za fiksiranje gonade, a repni dio u 10-postotni neutralni puferirani formalin.