natgillin/translations-raw huggingface dataset:
A larval counting basin and a number of 9 cm diameter petri dishes as in Chapter I(1), points (l) and (m).
Posuda za brojanje ličinki i veći broj petrijevih zdjelica promjera 9 cm, kao u poglavlju I. točkama 1. (l) i (m).
17,5 % hydrochloric acid.
17,5 % klorovodična kiselina.
Pepsin, strength:
Pepsin aktivnosti:
1:10000 NF (US National Formulary) corresponding to 1:12500 BP (British Pharmacopoeia) and to 2000 FIP (Fédération internationale de pharmacie), or stabilised liquid pepsin with minimum 660 European Pharmacopoeia units/ml.
1:10000 NF (US National Formulary) što odgovara 1:12500 BP (British Pharmacopoeia) i 2000 FIP (Fédération Internationale de pharmacie), ili stabilizirani tekući pepsin s najmanje 660 jedinica Europske farmakopeje/ml.
A number of 10 litre bins to be used for decontamination of apparatus, e.g. with formol, and for digestive juice remaining where specimens test positive.
Veći broj posuda od 10 litara za dekontaminaciju opreme, npr. pomoću formalina te za preostali probavni sok kada je test uzoraka pozitivan.
A balance accurate to 0,1 g.
Vaga s točnošću od 0,1 g.
As stipulated in Chapter I(2).
Kako je propisano u poglavlju I. točki 2.
I. Grinding
I. Mljevenje
Grinding the meat samples in a meat mincer beforehand will improve the digestion quality.
Ako se uzorci mesa prethodno samelju u stroju za mljevenje mesa, poboljšava se kvaliteta probave.
If an electrical blender is used, the blender must be operated three to four times for approximately one second each time.
Ako se koristi električna miješalica, ona se mora uključivati tri do četiri puta, svaki put po jednu sekundu.
Digestion procedure
Postupak probave
This procedure may involve complete pools (100 g of samples at a time) or pools of less than 100 g.
Ovaj se postupak može provoditi na skupnim uzorcima (istodobno 100 g uzoraka) ili na skupnim uzorcima manjim od 100 g.
Complete pools (100 samples at a time):
Skupni uzorci (100 g uzoraka istodobno):
The Stomacher lab-blender 3 500 is fitted with a double plastic bag and the temperature control set at 40 to 41 °C.
i. Na uređaj Stomacher lab-blender 3 500 stavlja se dvostruka plastična vrećica, a temperatura se podesi na 40 do 41 °C.
One and a half litres of water preheated to 40 to 41 °C is poured into the inner plastic bag.
U unutrašnju plastičnu vrećicu ulije se jedna i pol litra prethodno zagrijane vode na 40 do 41 °C.
25 ml of 17,5 % hydrochloric acid is added to the water in the Stomacher.
Vodi u Stomacheru doda se 25 ml 17,5 % klorovodične kiseline.
100 samples weighing approximately 1 g each (at 25 to 30 °C) taken from each individual sample in accordance with point 2 are added.
Tome se doda 100 uzoraka od kojih je svaki težak oko 1 g (na temperaturi od 25 do 30 °C), koji su uzeti iz pojedinačnih uzoraka u skladu s točkom 2.
Lastly, 6 g pepsin or 18 ml liquid pepsin is added.
v Na kraju se doda 6 g pepsina ili 18 ml tekućeg pepsina.
This order must be followed strictly to avoid decomposition of the pepsin.
Takav redoslijed mora se strogo poštovati da se spriječi razgradnja pepsina.
The Stomacher is then allowed to pound the content of the bag for 25 minutes.
Stomacher zatim 25 minuta miješa sadržaj vrećice.
The plastic bag is removed from the Stomacher and the digestion fluid is filtered through the sieve into a 3 litre beaker.
v. Plastična vrećica se izvadi iz Stomachera, a probavni sok se filtrira kroz sito u laboratorijsku čašu zapremnine 3 litre.
The plastic bag is washed with approximately 100 ml of water, which is then used to rinse the sieve and lastly added to the filtrate in the beaker.
Plastična vrećica se ispire s oko 100 ml vode, koja se zatim upotrijebi za ispiranje sita te se na kraju doda filtratu unutar laboratorijske čaše.
Up to 15 individual samples can be added to a total pool of 100 samples and examined together with these samples.
Skupnom uzorku od 100 uzoraka može se dodati do 15 pojedinačnih uzoraka i pregledati zajedno s tim uzorcima.
Smaller pools (less than 100 samples):
Manji skupni uzroci (manje od 100 uzoraka):
A digestion fluid is prepared by mixing about one and a half litres of water and 25 ml of 17,5 % hydrochloric acid.
Probavni sok se priprema miješanjem oko jedne i pol litre vode i 25 ml 17,5 % klorovodične kiseline.
6 g of pepsin is added and the whole mixed at a temperature of 40 to 41 °C.
Tome se dodaje 6 g pepsina i sve se pomiješa pri temperaturi od 40 do 41 °C.
Of the digestion fluid, a volume corresponding to 15 ml per gram of sample is measured (e.g. for 30 samples the volume required is 30 × 15 ml = 450 ml) and transferred to the inner of the two plastic bags, together with the meat samples weighing approximately 1 g (at 25 to 30 °C) taken from each individual sample in accordance with point 2.
Od probavnog soka se odvoji volumen koji odgovara omjeru od 15 ml po gramu uzorka (npr. za 30 uzoraka bio bi potreban volumen 30 × 15 ml = 450 ml) i stavi se u unutrašnju od dvije plastične vrećice zajedno s uzorcima mesa teškim oko 1 g (pri temperaturi od 25 do 30 °C), koji su uzeti iz svakog pojedinog uzorka u skladu s točkom 2.
Water at a temperature of approximately 41 °C is poured into the outer bag to make up a total volume in the two bags of one and a half litres.
U vanjsku se vrećicu ulije voda zagrijana na oko 41 °C, tako da ukupni volumen ovih vrećica bude jedna i pol litra.
The plastic bag is washed with approximately 100 ml of water (at 25 to 30 °C), which is then used to rinse the sieve and lastly added to the filtrate in the beaker.
Plastična vrećica se ispire s oko 100 ml vode (na 25 do 30 °C), koja se zatim upotrijebi za ispiranje sita te se na kraju doda filtratu unutar laboratorijske čaše.
Recovery of larvae by sedimentation
Sakupljanje ličinaka sedimentacijom
Ice (300 to 400 g of ice flakes, scaly ice or crushed ice) is added to the digestion fluid to bring its volume up to about 2 litres.
Probavnom soku dodaje se led (300 do 400 g leda u listićima ili zdrobljenog leda), tako da se njegov volumen poveća na oko 2 litre.
The digestion fluid is then stirred until the ice has melted.
Probavni sok se zatim miješa dok se ne otopi led.
In the case of smaller pools (see Section II(b)), the amount of ice must be reduced correspondingly.
U slučaju manjih skupnih uzoraka (pogledajte odjeljak II. (b)) količina leda mora se proporcionalno smanjiti.
The chilled digestion fluid is transferred to a 2 litre separation funnel, equipped with a vibrator in an extra clamp.
Rashlađeni probavni sok prenese se u separator zapremnine 2 litre, koji je opremljen napravom za vibraciju u posebnoj obujmici.
Sedimentation is allowed to proceed for 30 minutes, during which time the sedimentation funnel is vibrated intermittently, i.e. one minute vibration followed by a one-minute pause.
Tijekom sedimentacije koja traje 30 minuta, sedimentacijski lijevak vibrira s prekidima, tj. izmjenjuju se jedna minuta vibriranja i jedna minuta stanke.
After 30 minutes, a 60 ml sample of the sediment is quickly run off into a 100 ml measuring cylinder (the funnel is rinsed with detergent solution after use).
Nakon 30 minuta, 60 ml uzorka taloga brzo se odlije u mjerni cilindar od 100 ml (lijevak se nakon upotrebe ispere otopinom deterdženta).
The 60 ml sample is allowed to stand for at least 10 minutes, after which time the supernatant is withdrawn by suction to leave a volume of 15 ml, to be examined for presence of larvae.
60 ml uzorka ostavi se da odstoji 10 minuta, a zatim se površinski sloj tekućine pažljivo izdvoji usisavanjem, tako da ostane volumen od 15 ml za pregled na prisutnost ličinaka.
For suction, a disposable syringe, equipped with a plastic tube, can be used.
Za usisavanje se može upotrijebiti brizgalica za jednokratnu upotrebu, opremljena plastičnom cjevčicom.
The length of the tube must be such that 15 ml remains in the measuring cylinder when the flanges of the syringe rest on the cylinder's rim.
Dužina te cjevčice mora biti takva da, kada se krilca brizgalice postave na rub mjerenog cilindra, u cilindru ostane 15 ml.
The remaining 15 ml is poured into a larval counting basin or two petri dishes and examined using a trichinoscope or stereo-microscope.
Preostalih 15 ml izlijeva se u posudu za brojanje ličinki ili u dvije petrijeve zdjelice te se pretražuje upotrebom trihineloskopa ili binokularnog mikroskopa.
The measuring cylinder is washed with 5 to 10 ml of tap water and the washings are added to the sample.
Mjerni cilindar se ispere s 5 do 10 ml vode iz slavine i taj se ispirak doda uzorku.
Under no circumstances is examination to be postponed until the following day.
Ni u kojem se slučaju pretraga ne smije odgoditi za sljedeći dan.
Where the digests are unclear or they are not examined within 30 minutes of their preparation, they must be clarified as follows:
Kad sadržaj umjetne probave nije bistar ili se ne pregledava u roku od 30 minuta nakon njegove pripreme, on se mora razbistriti na sljedeći način:
the final sample of 60 ml is poured into a measuring cylinder and allowed to stand for 10 minutes;
konačni uzorak od oko 60 ml izlije se u mjerni cilindar i ostavi da odstoji 10 minuta;
45 ml of supernatant fluid is then removed by suction and the remaining 15 ml is made up to 45 ml with tap water,
zatim se usisavanjem otkloni 45 ml površinskog sloja tekućine, a preostalih 15 ml se nadopuni s 45 ml vode iz slavine,
after a further settling period of 10 minutes, 30 ml of supernatant fluid is removed by suction and the remaining 15 ml is poured into a petri dish or larval counting basin for examination,
nakon daljnjeg stajanja od 10 minuta, opet se isisa 30 ml površinskog sloja tekućine, a preostalih 15 ml za pregled ulije se u petrijevu zdjelicu ili u posudu za brojanje ličinki,
the measuring cylinder is washed with 10 ml of tap water and these washings are added to the sample in the petri dish or the larval counting basin for examination.
mjerni cilindar se ispere s 10 ml vode iz slavine i taj se ispirak dodaje uzorku za pregled u petrijevoj zdjelici ili u posudi za brojanje ličinki.
Where the result is positive or uncertain, the provisions laid down in Chapter I(3)(III) shall apply.
Kada je rezultat pozitivan ili sumnjiv, primjenjuju se odredbe utvrđene u poglavlju I. točki 3. III.
B. Mechanically assisted pooled sample digestion method/'on filter isolation' technique
B. Mehanička metoda umjetne probave skupnog uzorka/tehnika „izdvajanje na filteru”
As stipulated in Section A(1).
Kako je određeno u odjeljku A točki 1.
Additional equipment:
Dodatna oprema:
1 litre Gelman funnel, complete with filter holder (diameter 45 mm);
Gelmanov separator zapremnine 1 litre, opremljen držačem za filter (promjer 45 mm);
filter discs, consisting of a circular stainless steel mesh with an aperture of 35 microns (disc diameter: 45 mm), two rubber rings 1 mm thick (external diameter: 45 mm; internal diameter: 38 mm), the circular mesh being placed between the two rubber rings and bonded to them using a two-component glue suitable for the two materials;
filterske ploče koje se sastoje od okrugle mreže izrađene od nehrđajućeg čelika s porama od 35 mikrona (promjer ploče: 45 mm), dva gumena prstena debljine 1 mm (promjer vanjskog prstena: 45 mm; promjer unutrašnjeg prstena: 38 mm), s tim da se okrugla mreža postavi između dva gumena prstena i za njih pričvrsti upotrebom dvokomponentnog ljepila prikladnog za lijepljenje oba materijala;
an Erlenmeyer flask, capacity 3 litres, fitted with a side tube for suction;
Erlenmeyerova tikvica zapremnine 3 litre, opremljena postraničnom cjevčicom za usisavanje;
a filter pump;
filterska pumpa;
plastic bags, capacity at least 80 ml;
plastične vrećice, zapremnine najmanje 80 ml;
equipment for sealing the plastic bags;
naprava za plombiranje plastičnih vrećica;
rennilase, strength 1:150000 Soxhlet units per gram.
renilaza, jakosti 1: 150000 Sokslet jedinica na gram.
Collecting of specimens
Prikupljanje uzoraka
Complete pools (100 samples at a time)
Skupni uzorci (100 uzoraka istodobno)
See Section A(3)(II)(a).
Vidjeti odjeljak A. 3. II. (a).
Smaller pools (less than 100 samples)
Manji skupni uzroci (manje od 100 uzoraka)
See Section A(3)(II)(b).
Vidjeti odjeljak A. 3. II. (b).
Recovery of larvae by filtration
Sakupljanje ličinaka filtriranjem
In the case of smaller pools, the amount of ice must be reduced correspondingly.
Kada su uzorci manji, količina leda se mora proporcionalno smanjiti.
The digestion fluid is stirred until the ice has melted.
Probavni sok se zatim miješa dok se ne otopi led.
The chilled digestion fluid is then left for at least three minutes to let the larvae coil.
Rashlađena digestivna tekućina ostavi se najmanje tri minute da bi se ličinke sklupčale.
The Gelman funnel, fitted with a filter holder and filter disc, is mounted on an Erlenmeyer flask connected to a filter pump.
Gelmanov separator opremljen držačem za filter i diskom s filterom stavlja se na Erlenmeyerovu tikvicu koja je povezana s pumpom filtera.
The digestion fluid is poured into the Gelman funnel and filtered.
Probavni sok ulije se u Gelmanov separator i filtrira.
Towards the end of filtration, the digestion fluid can be helped to pass through the filter by applying suction with the filter pump.
Pred kraj filtriranja prolaz probavnog soka kroz filter može se potpomoći primjenom usisavanja pomoću pumpe filtra.
Suction must cease before the filter becomes dry, i.e. when 2 to 5 ml of fluid is left in the funnel.
Usisavanje se mora prekinuti prije nego što filter postane suh, tj. kada u lijevku ostane 2 do 5 ml tekućine.
Once all the digestion fluid has been filtered, the filter disc is removed and placed in an 80 ml capacity plastic bag, together with 15 to 20 ml of rennilase solution.
Kada je sav probavni sok profiltriran, disk s filterom se skida i stavlja u plastičnu vrećicu zapremnine 80 ml zajedno s 15 do 20 ml otopine renilaze.
The rennilase solution is made by adding 2 g of rennilase to 100 ml of tap water.
Otopina ranilaze priprema se dodavanjem 2 g renilaze u 100 ml vode iz slavine.
The plastic bag is sealed twice and placed between the inner and outer bags in the Stomacher.
Plastična vrećica se dva puta plombira i postavi između unutrašnje i vanjske vrećice Stomachera.
The Stomacher is allowed to pound for three minutes, e.g. while it is working on a complete or incomplete pool.
Pusti se Stomacher da usitnjava kroz tri minute, npr. dok radi na skupnom ili nepotpunom uzorku.
After three minutes, the plastic bag, complete with filter disc and rennilase solution, is removed from the Stomacher and opened with scissors.
Nakon tri minute, plastična se vrećica zajedno s diskom s filterom i otopinom renilaze vadi iz Stomachera i otvara škarama.
The liquid contents are poured into a larval counting basin or petri dish.
Tekući sadržaj se izlije u posudu za brojanje ličinki ili u petrijevu zdjelicu.
The bag is washed out with 5 to 10 ml of water, which is then added to the larval counting basin for examination by trichinoscope or to the petri dish for examination by stereo-microscope.
Vrećica se ispere s 5 do 10 ml vode, koja se zatim doda u posudu za brojanje ličinki u svrhu pregleda pomoću trihelonoskopa, ili u petrijevu zdjelicu u svrhu pretrage pomoću binokularnog mikroskopa.
Digests must be examined as soon as they are ready.
Sadržaj umjetne probave mora se pretražiti čim je gotov.
Filter discs must never be used when not completely clean.
Diskovi s filterom ne smiju se nikad koristiti ako nisu posve čisti.
Unclean discs must never be allowed to dry out.
Ne smije se nikad dozvoliti da se diskovi koji nisu čisti osuše.
Filter discs can be cleaned by leaving them in rennilase solution overnight.
Diskovi s filterom mogu se čistiti tako da se tijekom noći ostave uronjeni u otopinu renilaze.
Before use, they must be washed in fresh rennilase solution using the Stomacher.
Prije upotrebe, moraju se oprati u svježoj otopini renilaze upotrebom Stomachera.
C. Automatic digestion method for pooled samples of up to 35 g
C. Metoda automatske umjetne probave za skupni uzorak težak do 35 g
A Trichomatic 35® blender with filtration insert.
Miješalica Trichomatic 35® s umetkom za filtriranje.
Hydrochloric acid 8,5 ± 0,5 % weight.
Klorovodična kiselina težine 8,5 ± 0,5 %.
Transparent polycarbonate membrane filters with a diameter of 50 mm and a pore size of 14 microns.
Prozirni polikarbonatni membranski filteri s promjerom od 50 mm i veličinom pora od 14 mikrona.
Pepsin, strength 1:10000 NF (US National Formulary) corresponding to 1:12500 BP (British Pharmacopoeia) and to 2000 FIP (Fédération internationale de pharmacie), or stabilised liquid pepsin with minimum 660 European Pharmacopoeia units/ml.
Pepsin, aktivnosti 1:10000 NF (US National Formulary) što odgovara 1:12500 BP (British Pharmacopoeia) i 2000 FIP (Fédération internationale de pharmacie), ili stabilizirani tekući pepsin s najmanje 660 European Pharmacopoeia jedinica/ml.
Tweezers with a flat tip.
Pinceta s ravnim vrhovima.
A number of microscope slides with a side-length of at least 5 cm or a number of petri dishes at least 6 cm in diameter, marked on their undersides into 10 × 10 mm square areas using a pointed instrument.
Više mikroskopskih stakalaca s dužinom stranice najmanje 5 cm, ili više petrijevih zdjelica s promjerom najmanje 6 cm, koji su s donje strane zašiljenim instrumentom iscrtani u kvadrate veličine 10 × 10 mm.
A (stereo-)microscope with transmitted light (magnification 15 to 60 times) or a trichinoscope with a horizontal table.
Binokularni mikroskop s izvorom svjetlosti (povećanje 15 do 60 puta) ili trihineloskop s vodoravnom pločom.
A bin for collection of waste liquids.
Posuda za prikupljanje otpadnih tekućina.
I. Digestion procedure
I. Postupak probave
Place the blender with the filtration insert, connect the waste tube and place the tube so it drains into the waste bin.
Namjestite umetak za filtriranje u miješalicu, spojite odvodnu cijev i postavite je tako da se otpad odlijeva u posudu za otpad.
When the blender is switched on, heating will start.
Kada se miješalica uključi, započinje grijanje.
Before this is done, the bottom valve located below the reaction chamber must be opened and closed.
Prije toga treba otvoriti i zatvoriti donji ventil koji je smješten ispod reakcijske komore.
Up to 35 samples weighing approximately 1 g each (at 25 to 30 °C) taken from each individual sample in accordance with point 2 are then added.
Tada se dodaje do 35 uzoraka od kojih je svaki težak oko 1 g (na temperaturi od 25 do 30 °C), koji su uzeti iz pojedinačnih uzoraka u skladu s točkom 2.
Ensure that larger pieces of tendons are removed as they may clot the membrane filter.
Provjerite jesu li odstranjeni veći komadi tetiva, budući da oni mogu začepiti membranski filter.
Pour water up to the edge of a liquid chamber connected to the blender (approximately 400 ml).
Nalijte vodu do ruba komore za tekućinu koja je spojena s miješalicom (oko 400 ml).
Pour about 30 ml hydrochloric acid (8,5 %) to the edge of the smaller, connected liquid chamber.
Ulijte oko 30 ml klorovodične kiseline (8,5 %) do ruba manje, spojene komore za tekućinu.
Place a membrane filter under the coarse filter in the filter holder in the filter insert.
Postavite membranski filter ispod grubog filtera u držač uloška filtra.
Lastly, add 7 g of pepsin or 21 ml liquid pepsin.
Na kraju se doda 7 g pepsina ili 21 ml tekućeg pepsina.
Close the lids of the reaction and liquid chambers.
Zatvorite poklopac reakcijske komore i komore za tekućinu.
Select the period of digestion.
Odaberite vrijeme trajanja probave.
A short digestion period (5 minutes) must be set for pigs at the normal slaughter age and a longer time (8 minutes) for other samples.
Kratko vrijeme probave (5 minuta) mora se postaviti za svinje u normalnoj dobi za klanje dok se za druge uzorke postavlja duže vrijeme (8 minuta).
When the start button on the blender is turned on, the process of dispensing and digestion starts automatically, followed by filtration.
Kada se uključi tipka za početak na miješalici, postupak dijeljenja i probave počinje automatski, nakon kojeg slijedi filtriranje.
After 10 to 13 minutes the process is completed and stops automatically.
Nakon 10 do 13 minuta, postupak je završen i automatski se prekida.
Open the lid of the reaction chamber after checking that the chamber is empty.
Otvorite poklopac reakcijske komore nakon što ste provjerili da je komora prazna.
If there is foam or any digestion liquid remaining in the chamber, repeat the procedure in accordance with Section V.
Ako je u komori ostalo pjene ili bilo kojeg drugog probavnog soka, ponovite postupak u skladu s odjeljkom V.
Recovery of larvae
Prikupljanje ličinki
Remove the filter holder and transfer the membrane filter to a slide or petri dish.
Odstranite držač filtera i prenesite membranski filter na stakalce ili petrijevu zdjelicu.
Examine the membrane filter using a (stereo-)microscope or a trichinoscope.
Pretražite membranski filter upotrebom binokularnog mikroskopa ili trihineloskopa.
Cleaning equipment
Čišćenje opreme
Where the result is positive, fill the blender reaction chamber with boiling water until it is two-thirds full.
Kada je rezultat pozitivan, napunite dvije trećine reakcijske komore miješalice kipućom vodom.
Ordinary tap water is poured into the connecting liquid chamber until it covers the lower sensor.
Obična se voda iz slavine nalije u povezujuću komoru za tekućinu tako da pokrije donji senzor.
Automatic cleaning then takes place.
Tada započinje automatsko čišćenje.
Decontaminate the filter-holder and any other equipment, e.g. using formol.
Dekontaminirajte držač filetra i svu drugu opremu, npr. upotrebom formalina.
After work is completed for the day, fill the blender liquid chamber with water and put it through a standard cycle.
Kada je posao za taj dan gotov, napunite vodom komoru za tekućinu u miješalici i propustite je kroz standardni ciklus.
Use of membrane filters
Upotreba membranskog filtera
Each polycarbonate membrane filter may be used no more than five times.
Svaki polikarbonatni membranski filter može se upotrijebiti najviše pet puta.
The filter is to be turned between each use.
Filter se nakon svake upotrebe mora okrenuti.
In addition, the filter must be checked after each use for any damage which would make it unsuitable for further use.
Osim toga, treba ga nakon svake upotrebe provjeriti i utvrditi da nema nikakvih oštećenja zbog kojih bi bio neprikladan za daljnju upotrebu.
V. Method to be applied when digestion is incomplete and filtration cannot be carried out
V. Metoda koja se upotrebljava kada je probava nepotpuna i kada se ne može izvesti filtriranje
Once the blender has been put through an automatic cycle in accordance with Section I, open the lid of the reaction chamber and check whether there is foam or any liquid remaining in the chamber.
Kada je proveden automatski ciklus miješalice u skladu s odjeljkom I., otvorite poklopac reakcijske komore i provjerite je li u komori ostalo pjene ili bilo kakve tekućine.
If this is the case, proceed as follows:
U tom slučaju, učinite kao što slijedi:
close the bottom valve below the reaction chamber;
zatvorite donji ventil ispod reakcijske komore;
remove the filter holder and transfer the membrane filter to a slide or petri dish;
uklonite držač filtera i prenesite membranski filter na stakalce ili u petrijevu zdjelicu;
put a new membrane filter in the filter holder and attach the filter holder;
stavite novi membranski filter u držač filtera i pričvrstite držač filetra;
fill the blender liquid chamber with water until the lower sensor is covered;
komoru miješalice za tekućinu napunite vodom dok se ne pokrije donji senzor;
carry out the automatic cleaning cycle;
izvedite ciklus automatskog čišćenja;
after the cleaning cycle has ended, open the lid of the reaction chamber and check whether any liquid remains;
kada je ciklus automatskog čišćenja gotov, otvorite poklopac reakcijske komore i provjerite ima li preostale tekućine;
if the chamber is empty, remove the filter holder and transfer the membrane filter to a slide or petri dish with tweezers;
ako je komora prazna, izvadite držač filtera i pomoću pincete prenesite membranski filter na stakalce ili u petrijevu zdjelicu;
examine the two membrane filters in accordance with Section II.
pregledajte oba membranska filtera u skladu s odjeljkom II.