natgillin/translations-raw huggingface dataset:
Therefore it is justified to include embryos as appropriate genetic material for preservation of genetic base in gene bank from the very beginning.
Stoga je opravdano zametke kao pogodan genetski materijal za čuvanje genetske osnove u banci gena uključiti u program banke gena od samog početka.
Ova present suitable genetic material for storing in the Gene Bank.
Jajne stanice predstavljaju pogodan genetski materijal za čuvanje u Banci gena.
In the course of the last decade, protocols for collecting, freezing and activation of ova for larger number of domestic animals have been developed (cattle, sheep, goats, horses, pigs), while protocols for other species are under development.
Tijekom zadnjeg desetljeća razvijeni su protokoli za prikupljanje, zamrzavanje i aktiviranje jajnih stanica za veći broj vrsta domaćih životinja (goveda, ovce, koze, konji, svinje), dok se protokoli za druge vrste razvijaju.
Today, slow freezing protocols are used and vitrification of ova as well as technique of intracytoplasmic injection of sperm into ova for easier insemination.
Danas se koriste protokoli sporog smrzavanja i vitrifikacije jajnih stanica kao i tehnika intracitoplazmatskog injektivnog unosa sperme u jajnu stanicu radi lakše oplodnje.
Procedures with ova of fish and poultry had the least success.
Najmanje uspjeha je postignuto kod postupka s jajnim stanicama riba i peradi.
DNA analysis can help to continually update the knowledge on possible genetic flaws of individual animal and to determine its phylogenetic position on population.
Analizom DNA konstantno se mogu upotpunjavati spoznaje o mogućim genetskim nedostatcima jedinke te utvrđivati njihovu filogenetsku poziciju u populaciji.
Execution of facility
Izvedba postrojenja
Space needed by the Gene Bank depends on planned activities, type and amount of genetic material which will be collected and stored.
Veličina prostora koji iziskuje Banka gena ovisi o planiranim aktivnostima, vrsti i količini genetskog materijala koji će bit sakupljan i pohranjen.
Collecting of genetic material is undertaken in different secondary locations, while items 2.
Sakupljanje genetskog materijala provodi na različitim drugim mjestima, dok stavke 2.
Stated operations are supported by infrastructure of the Gene Bank.
Navedene poslove prati i infrastruktura Banke gena.
Within the Gene Bank, there is an area necessary for the purposes of short-term animal reception and harvesting of genetic material.
U sklopu Banke gena potrebno je imati prostor namijenjen kratkotrajnom prihvatu životinja i uzimanju genetskog materijala.
Laboratory is functional and practical.
Izvedba laboratorija je funkcionalna i praktična.
Walls, floors and work surfaces in laboratory are easy to wash and resistant to cleaning agents.
Zidovi, podovi i radne površine u laboratoriju trebaju biti lako perive i otporne na sredstva za čišćenje.
Laboratory should be well lit and have proper ventilation and a storage room for chemicals and additional equipment.
U laboratoriju treba osigurati dovoljno svijetla i kvalitetnu ventilaciju, spremište za kemikalije i dodatnu opremu.
Such arrangement enables higher degree of safety of the supplies and also disables unauthorized person's access.
Takav raspored omogućava veći stupanj sigurnosti zaliha, te ujedno onemogućava pristup osobama bez ovlasti.
Tanks should be placed on elevated stands due to protection from potential floods.
Dobro je da tankovi budu smješteni na povišenim postoljima jer su na taj način zaštićeni od potencijalnih poplava.
Each container is marked with a number on the lid.
Svaki spremnik je označen brojkom koja se nalazi na poklopcu.
Every container shall be properly marked with a label containing the following data:
Svaki spremnik treba biti pravilno označen naljepnicom sa slijedećim podatcima:
container number;
brojem spremnika,
indicated animal species;
naznačenoj životinjskoj vrsti,
hygienic status of the container (with date),
higijenski status spremnika (s datumom),
This increases physical safety of the collection.
Time se povećava fizička sigurnost kolekcije.
Liquid nitrogen presents potential health risk (from suffocation) for persons involved in filling of bottles and placing or extracting of samples, if leakage occurs.
Tekući dušik predstavlja potencijalni zdravstveni rizik (od gušenja) za osobe uključene u punjenje boca i stavljanje ili preuzimanje uzoraka, ako se dogodi istjecanje dušika.
Also, proper ventilation should be ensured in rooms with samples frozen with liquid nitrogen.
Isto tako, u prostorijama u kojima se čuvaju uzorci zamrznuti tekućim dušikom, potrebno je osigurati odgovarajuću ventilaciju.
As a precautionary measure, wearing of protective apron and gloves, closed shoes and protective glasses is mandatory.
Kao mjera sigurnosti važno je nošenje zaštitnih ogrtača i rukavica, zatvorenih cipela i zaštitnih naočala.
evaluation of genetic characteristics of animals for the purpose of choice for sampling,
ocjena genetskih karakteristika životinje u svrhu odabira za uzorkovanje,
repeated controls of deep-frozen semen (microscopic evaluation of progressive movability and sperm concentration after defrosting, viability, interest of pathomorphological sperm, % of live sperm, total number of sperm per dose etc.),
višekratne kontrole duboko smrznutog sjemena (mikroskopska ocjena progresivne pokretljivosti i koncentracije spermija nakon otapanja, vijabilitet, udio pato-morfoloških spermija, % živih spermija, ukupan broj spermija u dozi itd.),
determining the sex of embryos (where appropriate)
određivanje spola zametka (po potrebi),
laboratory area larger than 60 m2,
površina laboratorija veća od 60 m2,
storage room (or room for own production of liquid nitrogen).
prostorija za pohranu (ili vlastitu proizvodnju tekućeg dušika).
There is basic equipment which is upgraded with new one, as appropriate.
Postoji osnovna oprema, koja se po potrebi banke gena nadopunjava novom.
portable refrigerators (plastic or styrofoam, plastic blocks for freezing),
prijenosni hladnjaci (plastični ili od stiropora, plastični blokovi za zamrzavanje),
equipment for quality determination (concentration) of sperm (spectrophotometer,...)
oprema za određivanje kvalitete (koncentracije) sperme (spektrofotometar,…),
Additional equipment of gene bank and disposable materials are:
U dodatnu opremu banke gena i potrošni materijal ubraja se:
protective apron (overalls) and boots,
zaštitna pregača (kombinezon) i čizme,
gloves and feet covers for single use,
rukavice i navlake za noge za jednokratnu uporabu,
protective helmet (for example, for harvesting sperm of a stallion),
zaštitna kaciga (npr. prikupljanje sperme kod pastuha),
Gene Bank manager should compete graduate studies, and supporting staff should be educated for Gene Bank activities.
Voditelj Banke gena treba imati završen diplomski studij, a pomoćno osoblje treba educirano za poslove Banke gena.
Methods of collecting, processing, storing, preserving and distribution of the genetic material in the gene bank collection
Postupci prikupljanja, obrade, pohrane, čuvanja i distribucije genetskog materijala u zbirci banke gena
Componentsof documentationand information system of the Gene Bank
Komponente dokumentacijsko-informacijskog sustava Banke gena
quarantine status (30 days, in rooms designed for the purpose),
status karantene (30 dana, odvija se u posebnim, za to predviđenim prostorijama),
issuing of samples is defined under the protocol;
izdavanje uzoraka određeno je protokolom;
CRYO-IS HR is a component of CryoWEB documentation and information programme package for managing of the Gene Bank (http://cryoweb.tzv.fal.de/), which was so far installed in over 15 countries in Europe.
CRIO-IS HR je sastavnica CryoWEB dokumentacijsko-informacijskog programskog paketa za vođenje Banke gana (http://cryoweb.tzv.fal.de/), koji je do sada instaliran u više od 15 država na području Europe.
Main tasks of CRIO-IS HR programme package is to document and record stored genetic material in the Gene Bank, regardless the type of domestic animals or type of stored material (sperm, ova etc.)
Glavna zadaća CRIO-IS HR programskog paketa je dokumentiranje i evidencija pohranjenog genetskog materijala u Banku gena, bez obzira na vrstu domaćih životinja ili vrstu pohranjenog materijala (sperma, jajne stanice ili dr.).
storing - location (structure) of containers and freezers,
skladištenje - smještaj (struktura) spremnika i zamrzivača,
contact - list of persons responsible for operations of the gene bank.
kontakt - popis osoba odgovornih za rad banke gena.
in the form of a printed report.
u obliku tiskanog izvještaja.
Decision on preservation of breeds should be in accordance with the decisions of national health regulations which respect present diseases and risks in the animal sector, evaluation of the disease virulence degree and harmful effect on cattle production.
Odluka o očuvanju pasmina treba biti u suglasju s odlukama nacionalnih zdravstvenih propisa koji uvažavaju prisutne bolesti i rizike u animalnom sektoru, procjeni stupnja virulentnosti bolesti te štetnog utjecaja na stočarsku proizvodnju.
For easier management of the centre for collection of genetic material and reduction of possible disease occurrence, one principle should be observed:
Radi lakšeg upravljanja centrima za sakupljanje genetskog materijala i smanjenja mogućnosti pojave bolesti, treba uvažavati načelo:
Primary goal is to disable the transfer of disease into the station, or into the rooms for material collection, because the space and the equipment in this case are exposed to disease agents.
Primarni je cilj onemogućiti unošenje bolesti u stanicu, odnosno u prostorije za sakupljanje materijala, jer se interijer i sva oprema u tom slučaju izloženi uzročnicima bolesti.
Attention should be placed on portable material (portable refrigerators, collectors, medical equipment, cooling blocks for freezing etc.) which should be disinfected prior to new use and field work.
Pozornost treba obratiti na prijenosni materijal (prijenosni hladnjaci, kolektori, medicinska oprema, hladni blokovi za zamrzavanje, i dr.) koji trebaju biti dezinficirani prije novog korištenja i izlaska na teren.
If frozen material is delivered into the gene bank, nitrogen containers should be disinfected, followed by genetic material manipulation.
Ako se dostavlja zamrznuti materijal u banku gena, spremnici s duškom trebaju biti dezinficirani, nakon čega slijedi manipulacija s genetskim materijalom.
Personnel should use protective equipment defined by regulations: rubber boots or protective feet covers, protective aprons or overalls, rubber gloves etc.
Osoblje također treba koristiti zaštitnu opremu definiranu pravilnicima: gumene čizme ili zaštitne navlake za noge, zaštitne pregače ili mantile, gumene rukavice i dr.
Most of the protective equipment is designed for single use only.
Većina zaštitne opreme se jednokratno koristi.
Since quarantined animals sometime don't show visible symptoms of diseases, sampling of blood or other tissue is recommended (for example nose wipes) for complete tests for presence of infectious agents.
Kako životinje u karanteni ponekad ne pokazuju vidljive simptome bolesti, preporuča se uzimanje uzoraka krvi ili nekog drugog tkiva (npr. bris iz nosa) na cjelovitije testove za otkrivanje prisutnosti uzročnika bolesti.
Therefore, precautionary measures should be conducted in order to reduce the possibility of contamination.
Stoga sve mjere opreza da se umanji mogućnost kontaminacije trebaju biti poduzete.
Storing of samples of doubtful sanitary status (for example taken outside of planned collection location or animals not recoded by protocol, by persons unauthorized for collecting, etc.) is conducted in designed and specially marked containers with liquid nitrogen.
Skladištenje uzoraka upitnog sanitarnog statusa (npr. uzorci uzeti van predviđenog mjesta sakupljanja ili od jedinki koje nisu evidentirane protokolom, od strane osoba koje nemaju ovlasti za sakupljanje, itd.) je u predviđene i posebno označene spremnike s tekućim dušikom.
List of Autochthonous and Protected Breeds and Strains and their Needed Number (OG 127/98, 73/03, 39/06, 126/07, 70/09).
Popis izvornih i zaštićenih pasmina i sojeva domaćih životinja te njihov potrebit broj (NN 127/98, 73/03, 39/06, 126/07, 70/09).
Ordinance on Production, Sales and Marking of Genetic Material (OG 155/98)
Pravilnik o proizvodnji, prodaji i obilježavanju genetskog materijala (NN 155/98)
Ordinance on Veterinary Conditions for Traffic and Import of Bovine Semen (OG 60/08, 135/08)
Pravilnik o veterinarskim uvjetima za promet i uvoz sjemena goveda (NN 60/08, 135/08)
Ordinance on Veterinary Conditions Applied in Traffic and Import of Bovine Embryos (OG 74/08)
Pravilnik o veterinarskim uvjetima koji se primjenjuju u prometu i uvozu zametaka goveda (NN 74/08)
Ordinance on Identification and Registration of Biungulate animals (123/09)
Pravilnik o identifikaciji i registraciji kopitara (123/09)
Ordinance on Obligatory Marking and Registration of Cattle (OG 99/07, 41/08)
Pravilnik o obveznom označavanju i registraciji goveda (NN 99/07, 41/08)
Ordinance on Implementation of Obligatory Marking and Registration of Cattle (OG 81/11, 101/11)
Pravilnik o provođenju obveznog označavanja i registracije goveda (NN 81/11, 101/11)
Ordinance on Obligatory Marking and Registration of Sheep and Goats (OG 111/07, 135/08, 154/08, 81/11)
Pravilnik o obveznom označavanju i registraciji ovaca i koza (NN 111/07, 135/08, 154/08, 81/11)
Ordinance on Implementation of Obligatory Marking and Registration of Sheep and Goats (OG 111/07, 128/07, 154/08, 9/10, 110/10, 124/11)
Pravilnik o provođenju obveznog označavanja i registracije ovaca i koza (NN 111/07, 128/07, 154/08, 9/10, 110/10, 124/11 )
Ordinance on Obligatory Marking and Registration of Pigs (51/07, 50/08, 156/08, 148/09, 12/11)
Pravilnik o obveznom označavanju i registraciji svinja (51/07, 50/08, 156/08, 148/09, 12/11)
There are several approaches to the exchange of material between the gene bank and the animal owner, and the choice of most suitable one is on the team of professionals.
Za razmjenu genetskog materijala između banke gena i vlasnika životinje, postoji nekoliko pristupa, a odabir najpovoljnijeg odluka je tima stručnjaka.
Above listed approaches dictate drafting of agreement and/or contract on ownership over the genetic material.
Gore navedeni pristupi diktiraju sastavljanje sporazuma i/ili ugovora vlasništva nad genetskim materijalom.
Also, results of scientific researches and / or results of production indicators and their references, should be delivered to the Gene Bank for entry into the public data base.
Također, rezultati znanstvenih istraživanja i/ili rezultati proizvodnih pokazatelja i njihove reference, trebaju biti dostavljeni Banci gena za unos istih u javnu bazu podataka.
In this case main regulations are defined by the World Organisation for Animal Health (Office International des Epizooties, http://www.oie.int/)
U tom su slučaju glavni propisi definirani od strane Svjetske organizacije za zdravlje životinja (Office International des Epizooties, OIE, http://www.oie.int/).
Protocols for transfer of genetic material between countries are used by the signatory countries of the Sanitary and Phytosanitary measures (SPS, http://www.wto.org/english/tratop_e/sps_e/sps_e.htm), which should establish national sanitary and phytosanitary measures, compliant to the valid laws of the country and to adapt them to the international standards, guidelines and recommendations.
Protokole za prijenos genetskog materijala između zemalja, koriste i zemlje potpisnice Sanitarnih i fitosanitarnih mjera (Sanitary and Phytosanitary measures, SPS, http://www.wto.org/english/tratop_e/sps_e/sps_e.htm), koje trebaju uspostaviti nacionalne sanitarne i fitosanitarne mjere, sukladne važećim zakonima države te ih prilagoditi međunarodnim standardima, smjernicama i preporukama.
From examples of other countries which have initiated the Gene Bank project, it is possible to gain insight into positive and negative aspects of different project models and such experiences are recommended to be used for the purpose of financial, professional and scientific efficiency of this Project.
Iz primjera drugih država koje su pokrenule projekt Banke gena stječe se uvid u brojne pozitivne i negativne aspekte različitih projektnih modela te se takva iskustva preporuča iskoristiti u svrhu financijske, stručne i znanstvene efikasnosti ovoga Projekta.
project should be seen as the occurrence of permanent value which holds growth potential throughout the years,
projekt treba promatrati kao nastanak trajne vrijednosti koja ima potencijal rasta kroz godine,
quality management system should be introduced,
potrebno je uvesti sustav kontrole kvalitete (Quality management system),
Considering the options and available protocols of collection, manipulation, storing and activation of the stored tissue material, the approach is characteristic and compliant with the types of tissues, breed and other estimated parameters.
Obzirom na mogućnosti i raspoložive protokole prikupljanja, manipulacije, pohranjivanja i aktiviranja pohranjenog tkivnog materijala pristup je osobit i sukladan vrstama tkiva, pasmini, i drugim procijenjenim parametrima.
Collection, storing and use of the genetic material from the Gene Bank Collection is supervised and approved by the National Programme Council, according to the estimation based on received application, condition of population and risks.
Prikupljanje, pohranjivanje i korištenje genetskog materijala iz Zbirke Banke gena nadzire i odobrava Savjet NP, po procjeni temeljenoj na zaprimljenom zahtjevu, stanju populacije i rizicima.
Collection of the genetic material in the Gene Bank is continuously supplemented, while extracting of the genetic material from the Gene Bank is allowed only from the Reserve Collection, section comprising 35% of stored genetic collection.
Zbirka genetskog materijala u Banci gena stalno se nadopunjava, dok je izuzimanje genetskog materijala iz Banke gena dozvoljeno isključivo iz Pričuvne zbirke, dijela kolekcije koji čini 35% pohranjene genetske zbirke.
Artificial insemination programme is developed in the Republic of Croatia for most of the species, and this is why it is possible to efficiently and quickly apply the programme of collecting, storing and activation of sperm.
Program umjetne inseminacije razvijen je u Republici Hrvatskoj za većinu vrsta, radi čega je moguće učinkovito i brzo primijeniti program prikupljanja, pohrane i aktiviranja sperme.
Thus harvested sperm is, for instance with sheep, very suitable for dilution and use, while with some species it is a less suitable method.
Na ovakav način prikupljena sperma je primjerice kod ovaca vrlo pogodna za razrjeđivanje i uporabu, dok je kod nekih drugih vrsta manje pogodna metoda.
Storage of sperm as exclusive material in the gene bank is not suitable because this can cause losses in maternal component of genome (mitochondrial DNA, W heterochromosomes for poultry)
Pohranjivanje sperme kao isključivog materijala u banci gena nije pogodno jer se time može gubiti maternalna komponenta genoma (mitohondrijska DNA, spolni W kromosom kod peradi).
Collecting, storing and activation of embryos is used with some species as a routine method (cattle, sheep), while the protocols for collection, storing and activation of embryos of other species is under development (pigs, horses...)
Prikupljanje, pohranjivanje i aktiviranje zametaka kod nekih se vrsta koristi kao rutinska metoda (goveda, ovce), dok se protokoli za prikupljanje, čuvanje i aktiviranje zametaka kod drugih vrsta razvijaju (svinje, konji, …).
Although past protocols of activation of oocyte from ovaries brought good results with some species (sheep, cattle), it is necessary to continue with perfecting of the protocols in order to increase the efficiency of collecting, storing and transferring of the genetic material.
Premda dosadašnji protokoli aktiviranja oocita iz jajnika kod nekih vrsta (ovce, goveda) daju dobre rezultate, potrebno je dalje usavršavati protokole kako bi se povećala učinkovitost prikupljanja, pohrane i prijenosa genetskog materijala.
Ovaries, i.e., ova, are not sufficient for the reconstruction of a breed because they don't contain the Ychromosome.
Jajnici odnosno jajne stanice nisu dostatne za rekonstrukciju pasmine jer ne sadržavaju Ykromosom.
Somatic cells are easily stored in the Gene Bank Collection, as protocols for their freezing are relatively simple and filling of the Gene Bank with this type of tissue is fast and does not require significant expenses.
Tjelesne stanice lako se i jednostavno pohranjuju u Zbirku Banke gena, protokoli njihova zamrzavanja su relativno jednostavni i samo popunjavanje Banke gena ovakvim tkivom je brzo i ne iziskuje znatnije materijalne izdatke.
It is expected that the protocols and equipment for activating such material shall be upgraded in the future regarding efficiency and costs, therefore collecting and storing of somatic cells into the gene collection is recommended (for example dermal fibrobalasts etc.)
Očekujući da će se protokoli i oprema za aktiviranje ovakvog materijala u budućnosti unaprijediti u pogledu učinkovitosti i troškova, preporuka je prikupljanje i pohranjivanje tjelesnih stanica u zbirku gena (npr. fibroplasti kože i drugi).
Storing of isolated DNA into the Gene Bank is useful from several points of view.
Pohranjivanje izolirane DNA u Banku gena korisno je s nekoliko stanovišta.
DNA stored in the Gene Bank has complete genetic information on animals and as such it is suitable for its genetic characterisation which rules out the additional need to use other tissues from the Gene Bank for this purpose (embryos, sperm, ova etc.)
Pohranjena DNA u Banci gena sadrži kompletnu genetsku informaciju o jedinki i kao takva pogodna je za njenu genetsku karakterizaciju čime se isključuje dodatna potreba korištenja u tu svrhu drugih tkiva iz Banke gena (zametaka, sperme, jajnih stanica i drugog).
Besides, in the course of the last decade, certain experiences were gained regarding the transmission of DNA sequences between animals, which can potentially present a path of reconstruction of animal through its DNA.
Osim toga, tijekom proteklog desetljeća ostvarena su određena iskustva u pogledu prijenosa sekvenci DNA između jedinki, što potencijalno može predstavljati put rekonstrukcije jedinke iz njene DNA.
Techniques of gene transmission are under development and in the future, they can favour more efficient use of DNA sequences in preservation of the Gene Bank.
Tehnike prijenosa gena se razvijaju i u budućnosti mogu pogodovati učinkovitijem korištenju sekvenci DNA u očuvanju Banke gena.
Dynamic maintenance of the Gene Bank Collection is a steady and continued process which respects the needs and capacities of collecting of genetic material regarding the available space, equipment, financial resources, human resources, risks and other.
Dinamičko održavanje Zbirke Banke gena je stalan i kontinuiran proces koji uvažava potrebe i kapacitete prikupljanja genetskog materijala obzirom na raspoloživi prostor, opremu, financijske resurse, ljudske kapacitete, rizike i drugo.
Founding and maintenance of the Gene Bank Collection need constant education of staff which is in direct contact with the genetic material, and staff which manages the documentation and information system, staff of Partner institutions which develops the protocols and methods.
Uspostava i upravljanje Zbirkom Banke gena iziskuje konstantnu edukaciju osoblja koje je u neposrednom kontaktu s genetskim materijalom, osoblja koji vodi dokumentacijsko-informacijski sustav, osoblja Suradničkih institucija koje razvija protokole i metode.
Founding and operations of the Gene Bank is specific in view of size and capacity, type of equipment and stored material and number of employees which depends on the amount of the genetic material, number of species and breeds whose genetic material shall be stored in the Gene Bank Collection, and on available financial sources.
Uspostava i rad banke gena specifična je u pogledu veličine i kapaciteta, vrste opreme i pohranjivanog materijala te broja zaposlenika što ovisi o količini genetskog materijala, broju vrsta i pasmina čiji će genetski materijal biti pohranjen u Zbirku Banke gena, te o raspoloživim financijskim izvorima.