natgillin/translations-raw huggingface dataset:
Appearance of precipitate in the reaction mixture at the end of the incubation time, if observed.
741, pp.
United Nations (UN) (2013).
EPA, Office of Chemical Safety and Pollution Prevention (2011), „Chemical Substances of Unknown or Variable Composition, Complex Reaction Products and Biological Materials: UVCB Substances“, http://www.epa.gov/opptintr/newchems/pubs/uvcb.txt.
Adler et al.
(2012), „Development and validation of an in vitro micronucleus assay platform in TK6 cells“, Mutation Research, Vol.
Gerberick et al.
746/1, pp.
EC EURL-ECVAM (2013).
Ishidate (1983), „An Application of Acridine Orange Fluorescent Staining to the Micronucleus Test“, Mutation Research, Vol.
Recommendation on the Direct Peptide Reactivity Assay (DPRA) for skin sensitisation testing.
120/4, pp.
Jaworska et al.
Langlois (1983), „A simple fluorescent staining procedure for micronuclei and RNA in erythrocytes using Hoechst 33258 and pyronin Y“, Mutation Research, Vol.
Bauch et al.
120/4, pp.
Nukada et al.
Bryce, S.M. et al.
Ball et al (2011).
(2011), „Miniaturized flow cytometry-based CHO-K1 micronucleus assay discriminates aneugenic and clastogenic modes of action“, Environmental and Molecular Mutagenesis, 52/4, pp.
Landsteiner and Jacobs (1936).
Nicolette, J. et al.
FDA (Food and Drug Administration (2001).
Elhajouji, A., M. Cunha, M. Kirsch-Volders (1998), „Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay“, Mutagenesis, Vol.
ECETOC (2003).
13/2, pp.
Relevance incorporates consideration of the accuracy (concordance) of a test method (21).
Kirsch-Volders, M. et al.
Determination of instrument performance (e.g. sensitivity) by analysis of a reference standard prior to running the analytical batch (22).
Ryan, T. P. (2000), Statistical Methods for Quality Improvement.
The term “test chemical” is used to refer to what is being tested.
Paik (2003), Statistical Methods for Rates and Proportions, 3rd ed.
60-80
programmeeritud rakusurm, mida iseloomustab rida etappe, mis viivad raku lagunemiseni membraaniga seotud osakesteks, mis kõrvaldatakse fagotsütoosiga või osakeste raku membraanilt koorumise teel (shedding).
30-60
Kõik genotoksiliste toimete tüübid ei põhjusta mutatsioone või püsivat kromosoomikahjustust.
0-24
tähendavad uuritava kemikaali lõppkontsentratsioone söötmes.
0-7
Teoreetiliselt peaksid geenimutatsioonid või kromosoomaberratsioonid kergemini tekkima rakkudes, milles puudub funktsionaalne p53 valk ja mis seetõttu ei ole võimelised rakutsüklit peatama või kõrvaldama kahjustunud rakke apoptoosi või teiste DNA kahjustuse vallandatud ja p53 funktsioonidega seotud mehhanismide kaudu (nt DNA reparatsiooni indutseerimine).
0-25
Praegust prognoosimudelit ei saa kasutada tundmatu koostisega komplekssete segude või tundmatu või muutuva koostisega ainete, komplekssete reaktsioonisaaduste või bioloogilist päritolu materjalide (st unknown or variable composition, complex reaction products or biological materials, UCVB) puhul, sest selles katses peab uuritava kemikaali ja peptiidi molaarne suhe olema määratud.
10-45
OECD (2012), „The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins.
0-5,5
Part 1: Scientific Evidence“, OECD Environmental Health and Safety Publication Series on Testing and Assessment No.
The medium is then removed and replaced with fresh culture medium (150 μl culture medium containing serum but without Geneticin in the case of KeratinoSensTM) to which 50 μl of the 25 fold diluted test chemical and control substances are added.
Adler et al.
At least one well per plate should be left empty (no cells and no treatment) to assess background values.
(2011), „Alternative (non-animal) methods for cosmetics testing: current status and future prospects-2010“, Archives of Toxicology, Vol.
Acceptance criteria
Gerberick et al.
Emter R., Ellis G., Natsch A. (2010).
Jaworska et al.
Toxicology and Applied Pharmacology 245, 281-290.
(2013), „Bayesian integrated testing strategy to assess skin sensitization potency: from theory to practice“, Journal of Applied Toxicology, avaldatud internetis, 14. mai 2013, DOI: 10.1002/jat.2869.
Dinkova-Kostova A.T., Holtzclaw W.D., Kensler T.W.
Bauch et al.
18, 1779-1791.
(2012), „Putting the parts together: combining in vitro methods to test for skin sensitizing potential“, Regulatory Toxicology and Pharmacology 63: 489–504.
Kansanen E., Kuosmanen S.M., Leinonen H., Levonen A.L.
Nukada et al.
1(1), 45-49.
(2013), „Data integration of non-animal tests for the development of a test battery to predict the skin sensitizing potential and potency of chemicals“, Toxicology in Vitro 27: 609-618.
Natsch A., Bauch C., Foertsch L., Gerberick F., Normann K., Hilberer A., Inglis H., Landsiedel R., Onken S., Reuter H., Schepky A., Emter R. (2011).
Ball et al.
Journal of Applied Toxicology, 33, 1337-1352.
Dupuis and Benezra (1982), Allergic contact dermatitis to simple chemicals: a molecular approach, New York & Basel: Marcel Dekker Inc.
EURL-ECVAM (2014).
Lepoittevin et al.
Natsch A., Emter R., Ellis G. (2009).
(1998), Allergic contact dermatitis: the molecular basis.
Ball N., Cagen S., Carrillo J.C., Certa H., Eigler D., Emter R., Faulhammer F., Garcia C., Graham C., Haux C., Kolle S.N., Kreiling R., Natsch A., Mehling A. (2011).
Natsch et al.
Bauch C., Kolle S.N., Ramirez T., Eltze T., Fabian E., Mehling A., Teubner W., van Ravenzwaay B., Landsiedel R. (2012).
OECD (2005), „Guidance Document on the Validation and International Acceptance of New or Updated Test Methods for Hazard Assessment“, Series on Testing and Assessment No.
33, 1353-1364.
ECETOC (2003), „Contact sensitization: Classification according to potency“, European Centre for Ecotoxicology & Toxicology of Chemicals, (Technical Report No. 87).
Andres E., Sa-Rocha V.M., Barrichello C., Haupt T., Ellis G., Natsch A. (2013).
keemilised elemendid ja nende ühendid, kas looduslikud või tootmisprotsessiga saadud, sealhulgas neis sisalduvad toote stabiliseerimiseks vajalikud lisaained või tootmisprotsessist tulenevad lisandid, välja arvatud lahusti, mida on võimalik eraldada aine stabiilsust mõjutamata ja selle koostist muutmata (1).
Toxicology In Vitro 27, 1220-1225.
sündmuste ahel alates sihtkemikaali või sarnaste kemikaalide rühma keemilisest struktuurist molekulaarse initsieerimisetapi kaudu vaadeldava toime kliinilise avaldumiseni in vivo (2).
Fabian E., Vogel D., Blatz V., Ramirez T., Kolle S., Eltze T., van Ravenzwaay B., Oesch F., Landsiedel R. (2013).
Kalibreerimiskõver –
87, 1683-1969.
seos katses saadud toime väärtuse ja aine teadaoleva analüütilise kontsentratsiooni vahel (nimetatakse ka standardkõveraks).
Thorne N., Inglese J., Auld D.S.
struktureeritud lähenemisviis, mida kasutatakse kemikaali või kemikaalide rühma ohutegurite tuvastamiseks (võimalikkus), selle kirjeldamiseks (toime tugevus) ja/või ohutuse hindamiseks (võimalikkus / toime tugevus ja kokkupuude) ja millega ühendatakse strateegiliselt ning analüüsitakse kõik asjakohased andmed, et oleks olemas vajalik teave regulatiivse meetme võtmiseks seoses võimaliku ohu ja/või riskiga ja/või täiendavaks sihipäraseks (ja seega minimaalseks) katsetamiseks.
Chemistry and Biology 17, 646-657.
aine, mis on määratletud oma kvantitatiivse koostisega, milles on rohkem kui üks põhikoostisosa, mille kontsentratsioon on vahemikus 10 %–80 % (massiprotsent).
Gildea L.A., Ryan C.A., Foertsch L.M., Kennedy J.M., Dearman R.J., Kimber I., Gerberick G.F.
Mitme koostisosaga aine saadakse tootmisprotsessi käigus.
Dermatol., 126, 1813-1822.
Erinevus segu ja mitme koostisosaga aine vahel seisneb selles, et segu saadakse kahe või enama aine kokku segamisel, ilma et toimuks keemilist reaktsiooni.
Emter R., van der Veen J.W., Adamson G., Ezendam J., van Loveren H., Natsch A. (2013).
UVCB
NAFTA (North American Free Trade Agreement) (2012).
Vedelik
Technical Working Group on Pesticides — (Quantitative) Structure Activity Relationship ((Q)SAR) Guidance Document.
Sensibilisaator
Imax
Positiivne
The suggested NC chemical is calcium-containing (at a concentration of 1,0-1,8 mM), phenol red-free, HBSS (untreated, blank control).
30–60
A maximum leakage control should also be included in each run to allow for the calculation of FL20 values.
0–24
(mg/ml)
Bensülideenatsetoon
UN GHS C&L EU CLP C&L
122-57-6
U.S. EPA C&L
Tahke
UN (2009), United Nations Globally Harmonized System of Classification and Labelling of Chemicals (GHS), Third revised edition, New York & Geneva: United Nations Publications.
Sensibilisaator
EPA (1996), Label Review Manual: 2nd Edition, EPA737-B-96-001, Washington DC: U.S. Environmental Protection Agency.
(mõõdukas)
Scott, L. et al.
19317-11-4
In Vitro 24, 1-9
0–7
EC-ECVAM (1999), INVITOX Protocol 71: Fluorescein Leakage Test, Ispra, Italy: European Centre for the Validation of Alternative Methods (ECVAM).
United Nations (UN) (2013), Globally Harmonized System of Classification and Labelling of Chemicals (GHS), Fifth revised edition, UN New York and Geneva, 2013.
Ocular corrosive (a) A chemical that causes irreversible tissue damage to the eye.
, „Alternative (non-animal) methods for cosmetics testing: current status and future prospects-2010“, Archives of Toxicology 85, 367–485.
A chemical that produces a reversible change in the eye following application to the anterior surface of the eye; (b) Chemicals that are classified as UN GHS Categories 2A, or 2B; EU CLP Category 2; or U.S.
Emter R., Ellis G., Natsch A. (2010), „Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro“, Toxicology and Applied Pharmacology 245, 281–290.
EPA Categories II or III ocular irritants.
, „The role of Keap1 in cellular protective responses“, Chem.
EPA Category I ocular irritants.
Res.
A replicate containing all components of a test system and treated with a chemical known to induce a positive response.
Natsch A., Bauch C., Foertsch L., Gerberick F., Normann K., Hilberer A., Inglis H., Landsiedel R., Onken S., Reuter H., Schepky A., Emter R. (2011), „The intra- and inter-laboratory reproducibility and predictivity of the KeratinoSens assay to predict skin sensitizers in vitro: results of a ring-study in five laboratories“.
To ensure that variability in the positive control response across time can be assessed, the magnitude of the positive response should not be extreme.
Natsch A., Ryan C.A., Foertsch L., Emter R., Jaworska J., Gerberick G.F., Kern P. (2013), „A dataset on 145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation“, Journal of Applied Toxicology 33, 1337–1352.
Proficiency Chemicals A sub-set of the list of Reference Chemicals that can be used by a naïve laboratory to demonstrate proficiency with the validated reference test method.
Natsch A., Emter R., Ellis G. (2009), „Filling the concept with data: integrating data from different in vitro and in silico assays on skin sensitizers to explore the battery approach for animal-free skin sensitization testing“, Toxicol.
Relevance incorporates consideration of the accuracy (concordance) of a test method (8).
Bauch C., Kolle S.N., Ramirez T., Eltze T., Fabian E., Mehling A., Teubner W., van Ravenzwaay B., Landsiedel R. (2012), „Putting the parts together: combining in vitro methods to test for skin sensitizing potential“, Regul.
Reliability
Jaworska J., Dancik Y., Kern P., Gerberick F., Natsch A. (2013), „Bayesian integrated testing strategy to assess skin sensitization potency: from theory to practice“, J Appl Toxicol.
An untreated sample containing all components of a test system, including the solvent or vehicle that is processed with the test chemical-treated and other control samples to establish the baseline response for the samples treated with the test chemical dissolved in the same solvent or vehicle.
457, OECD, Paris.
When tested with a concurrent negative control, this sample also demonstrates whether the solvent or vehicle interacts with the test system.
, „Identification of gene expression changes induced by chemical allergens in dendritic cells: opportunities for skin sensitization testing“, J. Invest.
Specificity
, „Gene expressing changes in peripheral blood-derived dendritic cells following exposure to a contact allergen“, Toxicol.
The proportion of all negative/inactive chemicals that are correctly classified by the test.
Emter R., van der Veen J.W., Adamson G., Ezendam J., van Loveren H., Natsch A. (2013), „Gene expression changes induced by skin sensitizers in the KeratinoSens™ cell line: Discriminating Nrf2-dependent and Nrf2-independent events“, Toxicol. in Vitro 27, 2225–2232.
It is a measure of accuracy for a test method that produces categorical results and is an important consideration in assessing the relevance of a test method.
OECD (2015), „Performance Standards for assessment of proposed similar or modified in vitro skin sensitisation ARE-Nrf2 luciferase test methods“, OECD Environment, Health and Safety Publications Series on Testing and Assessment No.213, OECD, Paris.
Substance
OECD (2005), „Guidance Document on the Validation and International Acceptance of New or Updated Test Methods for Hazard Assessment“, OECD Environment, Health and Safety Publications, Series on Testing and Assessment No.23, OECD, Paris, France.
Test chemical Any substance or mixture tested using this test method.
Induktsiooni puudumisel on sensorvalgu Keap1 sihtmärk transkriptsioonifaktor Nrf2; sensorvalk põhjustab selle ubikvitinüleerimise ja proteolüütilise lõhustamise proteasoomis.
Tiered testing strategy
paralleelproov, mis sisaldab kõiki katsesüsteemi komponente peale uuritava kemikaali ning millele on lisatud kasutatav lahusti.
If the irritancy potential of a test chemical cannot be assigned based on the existing information, a step-wise sequential animal testing procedure is performed until an unequivocal classification can be made.
katsemeetodiga aja jooksul saadud tulemuste laborisisese ja laboritevahelise korratavuse ulatuse mõõt, kui katset tehakse sama metoodikaga.
It is important to note that a validated test method may not have sufficient performance in terms of accuracy and reliability to be found acceptable for the proposed purpose (8).
,5 (μM ) võrdlusvahemik [3]
Data from other tissues e.g. stomach/duodenum/jejunum, blood etc. could be collected at the same time.
(μM ) võrdlusvahemik [3]
The laboratory needs to demonstrate proficiency with each individual tissue in each species they are planning to study, and will need to demonstrate that an acceptable positive response with a known mutagen (e.g. EMS) can be obtained in that tissue.
Isopropanool
However careful consideration should be given to the logistics involved in tissue sampling for comet analysis alongside the requirements of tissue sampling for other types of toxicological assessments.
69-72-7
Harvest 24 hours after the last dose, which is typical of a general toxicity study, is not appropriate in most cases (see paragraph 40 on sampling time).
50-21-5
For each sample (per tissue per animal), at least 150 cells (excluding hedgehogs — see paragraph 56) should be analysed.
56-81-5
Scoring 150 cells per animal in at least 5 animals per dose (less in the concurrent positive control — see paragraph 29) provides adequate statistical power according to the analysis of Smith et al., 2008 (5).
35691-65-7
Changes in clinical chemistry measures (e.g. AST, ALT), can also provide useful information on tissue damage and additional indicators such as caspase activation, TUNEL stain, Annexin V stain, etc. may also be considered.
Tahke
However, there are limited published data where the latter have been used for in vivo studies and some may be less reliable than others.
Sensibilisaator (tugev)
Kirkland, D., G. Speit (2008), Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens III.
Positiivne
Appropriate follow-up testing in vivo, Mutation Research, Vol.
< 20
654/2, pp. 114-32.
20–100
Brendler-Schwaab, S. et al.
etüülaminofenoolsulfaat
20/4, pp. 245-54.
Tahke
Burlinson, B. et al.
Sensibilisaator (tugev)
, Fourth International Workgroup on Genotoxicity Testing: result of the in vivo Comet assay workgroup, Mutation Research, Vol.
Positiivne
627/1, pp. 31-5.
< 12,5
817, pp. 143-63.
2,4-dinitroklorobenseen
Smith, C.C. et al.
97-00-7
, Recommendations for conducting the in vivo alkaline Comet assay, Mutagenesis, Vol.
LUMINESTSENTSI MÕÕTMISE KVALITEEDIKONTROLL
18/1, pp. 45-51.
piisav tundlikkus, et kontrolliga kannude foon oleks stabiilne;
McKelvey-Martin, V.J. et al.
2 3 4 5 6 7 8 9
Singh, N.P. et al.
DMSO
, A simple technique for quantitation of low levels of DNA damage in individual cells, Experimental Cell Research, Vol.
DMSO
, Collaborative study on fifteen compounds in the rat-liver Comet assay integrated into 2- and 4-week repeat-dose studies, Mutation Research, Vol., 702/1, pp. 40-69.
DMSO
Olive, P.L., J.P.
DMSO
Tice, R.R., G.H.
DMSO
Strauss (1995), The single cell gel electrophoresis/Comet assay: a potential tool for detecting radiation-induced DNA damage in humans, Stem Cells, Vol.
DMSO
13/1, pp. 207-14.
DMSO
Collins, A.R (2004), The Comet assay for DNA damage and repair: principles, applications, and limitations, Molecular Biotechnology, Vol.
DMSO
26/3, pp. 249-61.
DMSO
, Evaluation of multi-organ DNA damage by Comet assay from 28 days repeated dose oral toxicity test in mice: A practical approach for test integration in regulatory toxicity testing, Regulatory Toxicology and Pharmacology, Vol.
DMSO
58/1, pp. 145–54.
MDCK CB997 rakkude monokiht saadakse, kasutades subkonfluentseid rakke, mida kasvatatakse rakukultuuripudelites söötmes DMEM / Nutrient Mix F12 (toitainete segu F12: L-glutamiini, 15 mM HEPESi, kaltsiumi (kontsentratsioonis 1,0–1,8 mM) ja 10 % kuumutamisega inaktiveeritud FCS/FBS (veise loote seerumi) 1 × kontsentraat).
722/1, pp. 7-19.
, „Development of an in vitro model to investigate repeat ocular exposure“, Ph.D.
O'Donovan, M., B. Burlinson (2013), Maximum dose levels for the rodent comet assay to examine damage at the site of contact or to the gastrointestinal tract, Mutagenesis, Vol.
silma eesmisele pinnale uuritava kemikaali kandmise järel silma sellise koekahjustuse või tugeva füüsilise nägemislanguse tekkimine, mis ei parane täielikult 21 päeva jooksul pärast kemikaali silmale kandmist.
Nesslany, F. (2007), In vivo Comet assay on isolated kidney cells to distinguish genotoxic carcinogens from epigenetic carcinogens or cytotoxic compounds, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol.
kemikaal, mis pärast silma eesmisele pinnale kandmist põhjustab selle pöörduva muutuse; b) kemikaal, mis klassifitseeritakse ÜRO GHSi kategooriasse 2A või 2B; ELi CLP-määruse 2. kategooriasse; või US EPA silmi ärritava toimega kemikaalide II või III kategooriasse.
630/1, pp. 28-41.
Tundlikkusega mõõdetakse katsemeetodi täpsust, kui saadud tulemusi saab kategoriseerida, ning see on oluline aspekt katsemeetodi asjakohasuse hindamisel (8).
393/1-2, pp. 175-8.
Täpsusega mõõdetakse katsemeetodi toimivust ja see on üks asjakohasuse aspektidest.
Toyoizumi, T. et al.
Usaldusväärsus
742/1-2, pp. 26-30.
Sasaki, Y.F. et al.
Wang, A. et al.
(2000), „The comet assay with multiple mouse organs: comparison of Comet assay results and carcinogenicity with 208 chemicals selected from the IARC monographs and U.S. NTP Carcinogenicity Database“, Critical Reviews in Toxicology, Vol.
, Measurement of DNA damage in rat urinary bladder transitional cells: improved selective harvest of transitional cells and detailed Comet assay protocols, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol.
30/6, pp.
634/ 1-2, pp. 51-9.
nukleoidide kuju pärast elektroforeesi, sarnasuse põhjal komeetidega: pea on tuum ja saba koosneb DNAst, mis on migreerunud tuumast välja elektrivälja toimel.
Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroup, Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol.
Kriitilise tähtsusega muutuja/näitaja
627/1, pp. 31-5.
Kriitilise tähtsusega muutujaid ei tohiks muuta, eriti ühe katse piires, võtmata arvesse, kuidas nende muutmine mõjutab katses uuritavat reaktsiooni, näiteks positiivse ja negatiivse kontrolliga saadud reaktsiooni ulatust ja varieeruvust.
Zheng, H., P.L.
Lahutamismeetodi kasutamise korral tuleks tuvastada ja kvantifitseerida nii lähteaine kui ka asjakohased metaboliidid [24] (vt punkt 65), kui BCFi või BMFi arvutamisel soovitakse aluseks võtta mitte radioaktiivselt märgistatud jääkide üldsisaldus, vaid lähteaine sisaldus kalas.
, Evaluation of DNA damage in different stages of mouse spermatogenesis after testicular X irradiation, Journal of Radiation Research, Vol. 160/4, pp. 443-451.
On siiski võimalik, et teave selliste hüdrofoobsete ainete vees lahustuvuse kohta ei ole põhjalik, mistõttu enne konkreetse katsemeetodi kasutamise üle otsuse langetamist tuleks uurida, kas on võimalik valmistada asjaomase aine püsiva, mõõdetava sisaldusega vesilahus (püsiv emulsioon ei ole lubatud), mida saaks kasutada veekaudse kokkupuute katses (16).
Merk, O., G. Speit (1999), Detection of crosslinks with the Comet assay in relationship to genotoxicity and cytotoxicity, Environmental and Molecular Mutagenesis, Vol.
Ei ole võimalik anda täpseid suuniseid sobiva katsemeetodi valimiseks lähtuvalt vees lahustuvuse ja süsteemis oktanool/vesi täheldatava jaotuskoefitsiendi piirkriteeriumidest, kuna eespool kirjeldatud põhjustel võivad muud tegurid (analüüsimeetod, lagunemine, adsorptsioon jne) avaldada märkimisväärset mõju katsemeetodi kohaldatavusele.
Pfuhler, S., H.U.
radioaktiivselt märgistatud ainete puhul märgistatud aatomi(te) täpne asukoht ja lisanditega seotud radioaktiivsuse protsentuaalne osakaal;
Wolf (1996), Detection of DNA-crosslinking agents with the alkaline Comet assay, Environmental and Molecular Mutagenesis, Vol.
Kasvu suhtes korrigeerimine toimub samal viisil kui täismahus katses.
27/3, pp. 196-201.
(Märkus: statsionaarse oleku saavutamiseks kuluva aja hindamise võrrandeid võib siin kasutada samamoodi kui veekaudse kokkupuute katse puhul – vt 5. liide.)
Jones (2012), Assessment of DNA interstrand crosslinks using the modified alkaline Comet assay, Methods in Molecular Biology, Vol.
The effect of cosolutes on the aqueous solubilities and octanol/water partition coefficients of selected polychlorinated biphenyl congeners.
605(1-2), pp. 7-16.
Partition controlled delivery of hydrophobic substances in toxicity tests using poly(dimethylsiloxane) (PDMS) films.
, Fourth International Workgroup on Genotoxicity Testing: result of the in vivo Comet assay workgroup, Mutation Research, Vol.627/1, pp. 31-5.
A simple apparatus for administering 2,3,7,8-tetrachlorodibenzo-p-dioxin to commercially available pelletized fish food.
, Comet Assay measurements: a perspective, Cell Biology and Toxicology, Vol.
Guidance for evaluating in vivo fish bioaccumulation data.
Møller, P. et al.
Pesticide analytical manual (PAM).
, Assessment and reduction of Comet assay variation in relation to DNA damage: studies from the European Comet Assay Validation Group, Mutagenesis, Vol.
kd.
Forchhammer, L. et al.
Väljaandes: Analysis of Pesticide Residues in Human and Environmental Samples.
, Variation in the measurement of DNA damage by Comet assay measured by the ECVAG inter-laboratory validation trial, Mutagenesis, Vol.
Toim.
25/2, pp. 113-23.
Thompson, J. F. USA Toidu- ja Ravimiamet, Research Triangle Park, NC, USA.
Azqueta, A. et al.
J. (1959).
, Towards a more reliable comet assay: Optimising agarose concentration, unwinding time and electrophoresis conditions, Mutation Research, Vol.
A rapid method of total lipid extraction and purification.
724/1-2, pp. 41-45.
A., Cichocki, E. A., ja Parrish, C. C. (1985).
723/2, pp. 87-90.
Smedes, F. (1999) Determination of total lipid using non-chlorinated solvents.
Ryan, T. P. (2000), Statistical Methods for Quality Improvement, John Wiley and Sons, New York 2nd ed.
Current approaches in the statistical analysis of ecotoxicity data: a guidance to application (ENV/JM/MONO(2006)18).