natgillin/translations-raw huggingface dataset:
Also, other anaerobic reactions occur by which substances may be at least partially degraded, e.g. by dechlorination, but this test does not detect such reactions.
Biolagunemise määr
However, by applying specific analytical methods for determining the test substance, its disappearance may be monitored (see paragraphs 6, 30, 44 and 53).
Biolagunemise protsent arvutatakse vabaruumis olevast gaasist võrrandi 10 järgi ja üldise biolagunemise protsent võrrandi 11 järgi:
Washed digested sludge [12], containing low (< 10 mg/l) concentrations of inorganic carbon (IC), is diluted about ten-fold to a total solids concentration of 1 g/l to 3 g/l and incubated at 35 °C ± 2 °C in sealed vessels with the test substance at 20 to 100 mg C/l for up to 60 days.
Võrrand 10
Allowance is made for measuring the activity of the sludge by running parallel blank controls with sludge inoculum in the medium but without test substance.
Võrrand 11
The increase in headspace pressure in the vessels resulting from the production of carbon dioxide and methane is measured.
Dh = biolagunemise protsent vabaruumis oleva gaasikoguse järgi;
Much of the CO2 produced will be dissolved in the liquid phase or transformed into carbonate or hydrogen carbonate under the conditions of the test.
Dt = summaarne biolagunemine ( %);
The amount of carbon (inorganic plus methane) resulting from the biodegradation of the test substance is calculated from the net gas production and net IC formation in the liquid phase in excess of blank control values.
Primaarse biolagunemise määr arvutatakse uuritava aine kontsentratsiooni mõõtmisest (soovi korral) inkubeerimise alguses ja lõpus võrrandi 12 järgi:
The extent of biodegradation is calculated from total IC and methane-C produced as a percentage of the measured or calculated amount of carbon added as test substance.
Võrrand 12
The course of biodegradation can be followed by taking intermediate measurements of gas production only.
uuritava aine primaarse biolagunemise protsent;
Additionally the primary biodegradation can be determined by specific analyses at the beginning and end of the test.
uuritava aine lähtekontsentratsioon (mg/l)
The purity, water solubility, volatility and adsorption characteristics of the test substance should be known to enable correct interpretation of results to be made.
uuritava aine kontsentratsioon katse lõpus (mg/l).
The organic carbon content (% w/w) of the test substance needs to be known either from its chemical structure or by measurement.
Kui analüüs näitab uuritava aine olulist kontsentratsiooni anaeroobse muda muutmata inokulumis, tuleb kasutada võrrandit 13:
For volatile test substances, a measured or calculated Henry's law constant is helpful in deciding whether the test is applicable.
Võrrand 13
Information on the toxicity of the test substance for anaerobic bacteria is useful in selecting an appropriate test concentration, and for interpreting results showing poor biodegradability.
uuritava aine primaarse biolagunemise parandatud protsent;
It is recommended to include the inhibition control unless it is known that the test substance is not inhibitory to anaerobic microbial activities (see paragraph 21 and ISO 13641-1 (12)).
uuritava aine „näiline” lähtekontsentratsioon (mg/l);
The test method may be applied to water-soluble substances;
uuritava aine „näiline” lõppkontsentratsioon (mg/l) tühikatsetes.
it may also be applied to poorly soluble and insoluble substances, provided that a method of exact dosing is used e.g. see ISO 10634 (13).
Tulemuste nõuetekohasus
In general, a case by case decision is necessary for volatile substances.
Tuleks kasutada ainult selliste nõude rõhu mõõtmise tulemusi, milles ei ole märgata roosat värvust (vt punkt 33).
Special steps may have to be taken, for example, not releasing gas during the test.
Anaeroobse käitlemise meetodite nõuetekohane kasutamine viib hapnikuga saastumise miinimumini.
To check the procedure, a reference substance is tested by setting up appropriate vessels in parallel as part of normal test runs.
Katse võib lugeda nõuetekohaseks ainult juhul, kui võrdlusaine jõuab platooni, mis vastab enam kui 60 % biolagunemisele [17].
Phenol, sodium benzoate and polyethylene glycol 400 are examples and would be expected to be degraded by more than 60 % theoretical gas production (i.e. methane and inorganic carbon) within 60 days (3)(14).
Kui pH katse lõpus oli läinud välja vahemikust 7 ± 1 ja biolagunemine oli ebapiisav, tuleb katset korrata katsekeskkonnaga, mille puhvermahtuvus on suurem.
REPRODUCIBILITY OF TEST RESULTS
Lagunemise pärssimine
In an international ring test (14) there was good reproducibility in gas pressure measurements between triplicate vessels.
Gaasi vabanemine nõudes, milles on nii uuritav aine kui ka võrdlusaine, peaks olema vähemalt sama suur kui nõudes, mis sisaldavad ainult võrdlusainet;
The relative standard deviation (coefficient of variation, COV) was mainly below 20 %, although this value often increased to > 20 % in the presence of toxic substances or towards the end of the 60-d incubation period.
vastasel korral on tegemist gaasi tekke pärssimisega.
Higher deviations were also found in vessels of volume < 150 ml.
Mõnel juhul on gaasi vabanemine uuritava ainega, kuid ilma võrdlusaineta nõus väiksem kui tühikatse kontrollnõus, mis näitab, et uuritaval ainel on inhibeeriv mõju.
Final pH values of the test media were in the range 6,5-7,0.
Katseprotokollis esitatakse alljärgnev teave.
The following results were obtained in the ring test.
tavanimetus, keemiline nimetus, CASi number, struktuurivalem ja asjakohased füüsikalis-keemilised omadused;
Test substance
uuritava aine puhtusaste (lisandid).
Total data
lahjendatud kääritusnõuvedeliku maht (Vl) ja nõu vabaruumi maht (Vh);
Mean degradation
katsenõude kirjeldus, biogaasi määramise kirjeldus (näiteks rõhumõõtja tüüp) ja anorgaanilise süsiniku analüsaatori kirjeldus;
(of total data)
uuritava aine ja võrdlusaine lisamine katsesüsteemi:
Relative Standard deviation
katses kasutatud kontsentratsioon ja lahustite kasutamine;
Valid data
reoveepuhasti nimi, reoveepuhasti kirjeldus (nt töötemperatuur, muda retentsiooniaeg, peamiselt olmereovesi jne), kontsentratsioon, igasugune teave, mis on vajalik selle põhjendamiseks ja teave inokulumi eeltöötlemise kohta (nt eelkääritamine, eelnevasse kokkupuutesse viimine);
(of valid data)
inkubatsioonitemperatuur;
Data > 60 % degradation in valid tests
pH ja anorgaanilise süsiniku sisaldus katse lõpus;
Palmitic acid
uuritava aine kontsentratsioon katse alguses ja lõpus, kui mõõdeti konkreetset ainet;
19 = 70 % [13]
kõik andmed, mis mõõdeti uuritava aine katses, tühikatses, võrdlusaine katses ja vajaduse korral inhibeerimise kontrollnõudes (nt rõhk millibaarides, anorgaanilise süsiniku kontsentratsioon (mg/l)) tabeli kujul (eraldi tuleb esitada vabaruumis ja vedelikus mõõdetud andmed);
Polyethylene Glycol 400
andmete statistiline analüüs, katse kestus ja biolagunemise kõver uuritava aine ja võrdlusaine ning inhibeerimise kontrollkatsete korral;
24 = 83 % [13]
uuritava aine ja võrdlusaine biolagunemise protsent;
The coefficients of variation of the mean for all values obtained with palmitic acid and polyethylene glycol 400 were as high as 45 % (n = 36) and 35 % (n = 38) respectively.
iga katsetulemuste arvestamata jätmise põhjendus;
When values of < 40 % and > 100 % were omitted (the former being assumed to be due to sub-optimal conditions, the latter due to unknown reasons), the COVs were reduced to 26 % and 23 %, respectively.
tulemuste arutelu.
The proportions of “valid” values attaining at least 60 % degradation were 70 % for palmitic acid and 83 % for polyethylene glycol 400.
Käesoleva lisa järgmised peatükid:
The proportions of the percentage biodegradation derived from DIC measurements were relatively low but variable.
C.4, Kohese biolagunduvuse määramine;
For palmitic acid the range was 0-35 %, mean 12 %, with COV of 92 % and for polyethyleneglycol 400 0-40 %, mean 24 %, with COV of 54 %.
Modified MITI Test (II), OECD Guideline for Testing of Chemicals, No. 302C, OECD, Paris
Incubator — spark-proof and controlled at 35 °C ± 2 °C;
Bioassay for monitoring biochemical methane potential and anaerobic toxicity.
Pressure-resistant glass test vessels of an appropriate nominal size [14], each fitted with a gas-tight septum, capable of withstanding about 2 bar.
Water Res.
The headspace volume should be about 10 % to 30 % of the total volume.
13, 485-492.
If biogas is released regularly, about 10 % headspace volume is appropriate, but if the gas release is made only at the end of the test 30 % is appropriate.
1 rõhumõõtur
Glass serum bottles, of nominal volume 125 ml, total volume around 160 ml, sealed with serum septa [15] and crimped aluminium rings are recommended when the pressure is released at each sampling time;
5 vabaruum (Vh)
Pressure-measuring device [16] adapted to enable measurement and venting of the gas produced, for example, a hand-held precision pressure meter connected to a suitable syringe needle;
6 läbikääritatud muda inokulum (Vl)
a 3-way gas-tight valve facilitates the release of excess pressure (Appendix 1).
Rõhumõõturi näitusid võib gaasi ruumalaga siduda standardkõvera abil, mis saadakse teadaoleva ruumalaga õhukoguste süstimisega 35 ± 2 °C juures seerumipudelitesse, milles on reaktsioonisegu ruumalaga VR võrdne kogus vett:
Note — The pressure readings are used directly to calculate the amount of carbon produced in the headspace (paragraphs 42 to 44).
Pudelid suletakse ja pannakse veevanni 35 °C juurde 1 tunniks tasakaalustuma;
Alternatively, the pressure readings may be converted to volumes (at 35 °C, atmospheric pressure) of gas produced using a conversion graph.
süstlanõel torgatakse läbi ühe nõu membraani, avatakse kraan, kuni rõhumõõtur näitab rõhku 0 ja suletakse siis kraan;
This graph is constructed from data obtained by injecting known volumes of nitrogen gas into a series of test vessels (e.g. serum bottles) at 35° +/– 2 °C and recording the resulting stabilised pressure readings (See Appendix 2).
sama korratakse ülejäänud pudelitega;
The calculation is shown in the Note in paragraph 44.
kogu seda tegevust korratakse 2 ml, 3 ml, 4 ml, 5 ml, 6 ml, 8 ml, 10 ml, 12 ml, 16 ml, 20 ml ja 50 ml õhuga;
Warning — Take care to avoid needle-stick injuries when using micro-syringes.
koostatakse kõver rõhunäitude (Pa) sõltuvuse kohta sissesüstitud gaasi mahust (ml).
Carbon analyser, suitable for the direct determination of inorganic carbon in the range of 1 mg/l to 200 mg/l;
Lagunemiskõvera näide (kumulatiivne netorõhu suurenemine)
Syringes of high precision for gaseous and liquid samples;
Anaeroobse biolagunemise katse näidisandmelehed – andmeleht uuritava aine kohta
Magnetic stirrers and followers (optional);
pH (lõpp) Labor:
Glove box (recommended).
Katse nr:
Distilled or deionised water (de-oxygenated by sparging with nitrogen gas containing less than 5 μl/l oxygen), containing less than 2 mg/l dissolved organic carbon (DOC).
Katsetemperatuur, °C:
Prepare the dilution medium to contain the following constituents at the stated amounts;
Vabaruumi maht (Vh):
Anhydrous potassium dihydrogen phosphate (KH2PO4)
Vedelfaasi maht (Vl ):
Disodium hydrogen phosphate dodecahydrate (Na2HPO4 · 12H2O))
Süsinikku uuritavas aines Cc,v:
Ammonium chloride (NH4Cl)
Päev
Calcium chloride dihydrate (CaCl2·2H2O)
p1 (katse)
0,075 g
p2 (katse)
Magnesium chloride hexahydrate (MgCl2·6H2O)
p3 (katse)
Iron (II) chloride tetrahydrate (FeCl2·4H2O)
p (katse)
Resazurin (oxygen indicator)
p4 (tühikatse)
0,001 g
p5 (tühikatse)
Sodium sulphide nonahydrate (Na2S·9H2O)
p6 (tühikatse)
Add de-oxygenated water (paragraph 15)
p (neto)
to 1 litre
katse – tühikatse
Freshly supplied sodium sulphide should be used or it should be washed and dried before use, to ensure sufficient reductive capacity.
keskmine (mbar)
The test may be performed without using a glove box (see paragraph 26).
Δp (neto)
Titanium (III) citrate is a highly effective and low-toxicity reducing agent, which is prepared as follows:
katse
Dissolve 2,94 g of trisodium citrate dihydrate in 50 ml of de-oxygenated water (to result in a solution of 200 mmol/l) and add 5 ml of a 15 % (w/v) titanium (III) chloride solution.
katse keskmine
Neutralise to pH 7 ± 0,2 with mineral alkali and dispense to an appropriate vessel under a stream of nitrogen.
tühikatse
The concentration of titanium (III) citrate in this stock solution is 164 mmol/l.
tühikatsete keskmine
Mix the components of the test medium except the reducing agent (sodium sulphide titanium citrate) and sparge the solution with nitrogen gas for about 20 min immediately before use to remove oxygen.
katse – tühikatse
Then add the appropriate volume of freshly prepared solution of the reducing agent (prepared in de-oxygenated water) just before use of the medium.
vedelfaasis C [4](mg)
Adjust the pH of the medium, if necessary, with dilute mineral acid or alkali to 7 ± 0,2.
kokku C [5](mg)
Stock solution of trace elements (optional)
Biolagunemine [6](%)
It is recommended that the test medium should contain the following trace elements to improve anaerobic degradation processes, especially if low concentrations (e.g. 1g/l) of inoculum are used (11).
C (lõpp)
Manganese chloride tetrahydrate (MnCl2 · 4H2O)
pH (lõpp)
50 mg
Võrdlusaine:
Boric acid (H3BO3)
Vedelfaasi maht (Vl ):
5 mg
Süsinikku võrdlusaines Cc,v (mg/l):
Zinc chloride (ZnCl2)
p1 (võrdlus)
Copper (II) chloride (CuCl2)
p2 (võrdlus)
3 mg
p3 (võrdlus)
Disodium molybdate dihydrate (Na2MoO4 · 2H2O)
p (võrdlus)
Cobalt chloride hexahydrate (CoCl2 · 6H2O)
võrdlusaine – tühikatse
100 mg
kumulatiivne
Nickel chloride hexahydrate (NiCl2 · 6H2O)
vabaruumi C [8](mg)
10 mg
Biolagunemine [9](%)
Disodium selenite (Na2SeO3)
võrdlusaine
Add the test substance as a stock solution, suspension, emulsion, or directly as solid or liquid, or as absorbed on to glass-fibre filter to give a concentration of no more than 100 mg/l organic carbon.
võrdlusaine keskmine
If stock solutions are used, prepare a suitable solution with water (paragraph 15) (previously de-oxygenated by sparging with nitrogen gas) of such a strength that the volume added is less than 5 % of the total volume of reaction mixture.
inhib. keskmine
Adjust the pH of the stock solution to pH 7 ± 0,2 if necessary.
võrdlusaine – inhib.
For test substances which are insufficiently soluble in water, consult ISO 10634 (13).
vedelfaasis C [10](mg)
If a solvent is used, prepare an additional control, with the solvent only added to the inoculated medium.
kokku C [11](mg)
Organic solvents which are known to inhibit methane production, such as chloroform and carbon tetrachloride, should be avoided.
Biolagunemine [12](%) Anorg.
Warning — Handle with care toxic test substances, and those whose properties are not known.
Süsinikku katsenõus, mv (mg):
Reference substances
mv = CC,v × Vl
Reference substances such as sodium benzoate, phenol and polyethylene glycol 400 have been used successfully to check the procedure, being biodegraded by more than 60 % within 60 days.
Süsinikku vabaruumis, mh (mg) normaalsel inkubeerimistemperatuuril (35 °C):
Prepare a stock solution (in de-oxygenated water) of the chosen reference substance in the same way as for the test substance and adjust to pH 7 ± 0,2 if necessary.
Biolagunemine, mis on arvutatud vabaruumis olevast gaasist, Dh (%):
Inhibition control (conditional)
Dh = (mh × 100)/mvv
In order to obtain information on the toxicity of the test substance to anaerobic micro-organisms to find the most appropriate test concentration, add the test substance and reference substance to a vessel containing the test medium (see paragraph 16), each at the same concentrations as added, respectively (see paragraphs 19 and 20 and see also ISO 13641-1 (12)).
Süsinikku vedelfaasis,ml (mg):
Digested sludge
Gaasistatud süsiniku kogusisaldus, mt (mg):
Collect digested sludge from a digester at a waste water treatment plant which treats predominantly domestic sewage.
Summaarne biolagunemine, Dt (%):
The sludge should be fully characterised and its background information should be reported (see paragraph 54).
LEOSTUMINE MULLAKOLONNIS
If use of adapted inoculum is intended, digested sludge from an industrial sewage treatment plant may be considered.
Käesolev katsemeetod on samaväärne OECD katsejuhendiga nr 312 (2004).
Use wide-necked bottles constructed from high-density polyethylene or a similar material, which can expand, for the collection of the digested sludge.
Sünteetilised kemikaalid satuvad pinnasesse kas otse, teadliku pinnasesse viimisega (nt põllumajanduskemikaalid) või kaudselt (nt reovesi → reoveesete → muld või õhk → märg- või kuivsadenemine).
Add sludge to within about 1cm of the top of the bottles and seal tightly, preferably with a safety valve.
Selliste kemikaalide riskihindamiseks on oluline eelnevalt hinnata seda, kas need kemikaalid võivad mullas muunduda ning liikuda (leostuda) edasi mulla sügavamatesse kihtidesse ja lõpuks põhjavette.
After transport to the laboratory, the collected sludge may be used directly or placed in a laboratory-scale digester.
Kemikaalide võimaliku mullas leostumise mõõtmiseks kontrollitud laboritingimustes on mitu meetodit, näiteks mulla õhukese kihi kromatograafia, mulla paksu kihi kromatograafia, mulla kolonnkromatograafia ning adsorptsiooni ja desorptsiooni mõõtmised (1, 2).
Release excess biogas by opening bottles of sludge carefully.
Mitteioniseerunud kemikaalide korral võimaldab n-oktanooli-vee jaotuskoefitsient (Pow) varakult hinnata nende võimalikku adsorptsiooni ja leostumist (3, 4, 5).
Alternatively, laboratory-grown anaerobic sludge may be used as a source of inoculum but its spectrum of activity may have been impaired.
Käesolevas katsemeetodis kirjeldatud meetod põhineb häiritud mulla kolonnkromatograafial (mõisted vt 1. liide).
Warning — Digested sludge produces flammable gases which present fire and explosion risks:
Tehakse kahte tüüpi katseid, et määrata kontrollitud laboritingimustes i) uuritava kemikaali võimalik leostumine mullas, ja ii) kemikaali muundumissaaduste võimalik leostumine mullas (uuring vanandatud jääkidega) [18].
In order to reduce background gas production and to decrease the influence of the blank controls, pre-digestion of the sludge may be considered.
Katsemeetod põhineb olemasolevatel meetoditel (6, 7, 8, 9, 10, 11).
If pre-digestion is required, the sludge should be allowed to digest without the addition of any nutrients or substrates at 35 °C ± 2 °C for up to 7 days.
Käesolevas katses kasutatavate mullaproovide arvu ja tüüpide osas lepiti kokku mulla ja sette valimist käsitlevas OECD töörühmas, mis tuli kokku Itaalias Belgirates 1995. aastal (12).
It has been found that pre-digestion for about 5 days usually gives an optimal decrease in gas production of the blank without unacceptable increases in either lag or incubation periods during the test phase or loss of activity towards a small number of substances tested.
Samuti andis töörühm soovitusi mullaproovide kogumise, käsitsemise ja säilitamise kohta leostumiskatsete puhul
For test substances which are, or are expected to be, poorly biodegradable, consider pre-exposure of the sludge to the test substance to obtain an inoculum which is better adapted.
Kolonnid, mis on valmistatud sobivast inertsest materjalist (nt klaas, roostevaba teras, alumiinium, teflon, polüvinüülkloriid jne), täidetakse mullaga ja seejärel küllastatakse ja tasakaalustatakse „kunstliku vihma” lahusega (mõiste vt 1. liide) ja nõrutatakse.
In such a case, add the test substance at an organic carbon concentration of 5 mg/l to 20 mg/l to the digested sludge and incubated for up to 2 weeks.
Seejärel kantakse iga mullakolonni pinnale uuritavat kemikaali ja/või uuritava kemikaali vanandatud jääke.
Wash the pre-exposed sludge carefully before use (see paragraph 25) and indicate in the test report the conditions of the pre-exposure.
Seejärel hakatakse mullakolonni voolutama kunstliku vihmaga ja kogutakse nõrgvesi.
Wash the sludge (see paragraphs 22 to 24) just prior to use, to reduce the IC concentration to less than 10 mg/l in the final test suspension.
Pärast leostamisprotsessi eemaldatakse muld kolonnist ja jagatakse sobivaks arvuks osadeks olenevalt sellest, millist teavet tahetakse saada.
Centrifuge the sludge in sealed tubes (e.g. 3000 g during 5 min) and discharge the supernatant.
Iga mullasamba osa ja nõrgvett analüüsitakse uuritava kemikaali ja vajaduse korral muundumissaaduste või muude huvipakkuvate kemikaalide määramiseks.
Suspend the resulting pellet in de-oxygenated medium (paragraphs 16 and 17), re-centrifuge the suspension and discharge the supernatant liquid.
Katsemeetodit saab kasutada (märgistamata või radioaktiivse märgisega, nt 14C) uuritavate kemikaalide puhul, mille jaoks on olemas piisava täpsuse ja tundlikkusega määramismeetodid.
If the IC has not been sufficiently lowered, the washing procedure of the sludge could be repeated twice as a maximum.
Katsemeetodit ei kasutata kemikaalide puhul, mis lenduvad kergesti pinnasest ja veest ning mida seepärast ei jää selle katsemeetodi tingimustes mulda ega nõrgvette.
This does not appear to affect the micro-organisms adversely.
Mullakolonnis leostumise mõõtmiseks võib kasutada märgistamata või radioaktiivse märgisega uuritavat kemikaali.
Finally, suspend the pellet in the requisite volume of test medium and determine the concentration of total solids [e.g. ISO 11923 (15)].
Radioaktiivse märgisega ainet on vaja selleks, et uurida muundumissaaduste (vanandatud uuritava kemikaali jäägid) leostumist ja määrata massibilanssi.
The final concentration of total solids in the test vessels should be in the range of 1 g/l to 3 g/l (or about 10 % of that in undiluted digested sludge).
Soovitatakse kasutada14C-märgist, kuid muud isotoobid nagu13C,15N,3H,32P võivad samuti olla kasulikud.
Conduct the above operations in such a way that the sludge has minimal contact with oxygen (e.g. use a nitrogen atmosphere).
Kuivõrd see on võimalik, tuleks märgis paigutada molekuli kõige stabiilsema(te) osa(de) külge.
Perform the following initial procedures using techniques to keep the contact between digested sludge and oxygen as low as practicable, for example, it may be necessary to work within a glove box in an atmosphere of nitrogen and/or purge the bottles with nitrogen (4).
Uuritava kemikaali puhtus peab olema vähemalt 95 %.
Preparation of test and control assays
Enamik kemikaale tuleks peale kanda eraldi ainena.
Prepare at least triplicate test vessels (see paragraph 13-b) for the test substance, blank controls, reference substance, inhibition controls (conditional) and pressure control chambers (optional procedure) (see paragraphs 7, 19 to 21).
Taimekaitsevahendite koostises oleva toimeaine seostumise uurimiseks võib kasutada ka valmistoodet, kuid uuritava kemikaali enda uurimine on eriti vajalik siis, kui segu tõenäoliselt mõjutab vabanemiskiirust (nt granuleeritud või toimeaine ohjatud eraldumisega tooted).
Additional vessels for the purpose of evaluating primary biodegradation using test substance specific analyses may also be prepared.
Seoses konkreetset toodet arvestavate võimalike erinõuetega katse korralduse suhtes võib olla kasulik pidada enne katse tegemist nõu reguleeriva asutusega.
The same set of blank controls may be used for several test substances in the same test as long as the headspace volumes are consistent.
Vanandatud jääkide leostumise uuringu puhul tuleks kasutada puhast lähtekemikaali.
Prepare the diluted inoculum before adding it to the vessels e.g. by the means of a wide-mouthed pipette.
Enne mullakolonnis leostumise katse tegemist peaks uuritava aine kohta olema kogutud järgmine teave:
Add aliquots of well-mixed inoculum (paragraph 25) so that the concentration of total solids is the same in all vessels (between 1 g/l and 3 g/l).
lahustuvus vees [katsemeetod A.6] (13);
Add stock solutions of the test and reference substance after adjustment to pH 7 ± 0,2, if necessary.
lahustuvus orgaanilistes lahustites;
The test substance and the reference substance should be added using the most appropriate route of administration (paragraph 19).
aururõhk [katsemeetod A.4] (13) ja/või Henry konstant;
The test concentration of organic carbon should normally be between 20 and 100 mg/l (paragraph 4).
jaotuskoefitsient süsteemis n-oktanool-vesi [katsemeetodid A.8 ja A.24] (13);