natgillin/translations-raw huggingface dataset:
Using a 250 μm mesh will prevent the worms from crawling into or through the mesh;
Tavaliselt visatakse kattev veekiht pärast konditsioneerimist ära.
however, care should be taken that as little as possible of the sediment particles is retained on the mesh.
settes sisalduva uuritava kemikaali kontsentratsioon, mille puhul bioloogilisele näitajale avalduva mõju ulatus on kindlaksmääratud kokkupuuteperioodi jooksul X % (nt 50 %).
The sieved slurry of each replicate vessel may be passed through the sieve a second time in order to ensure that all worms are recovered.
Tasakaalustusperiood periood, mille jooksul lastakse uuritaval kemikaalil jaotuda tahke faasi, poorivee ja sette kohal oleva vee vahel;
An alternative method could be warming of the sediment by placing the test vessels in a water bath at 50 – 60 °C;
see toimub pärast settele uuritava kemikaali lisamist ja enne katseorganismide lisamist.
the worms will leave the sediment and can be collected from the sediment surface by use of a fire-polished wide-mouth pipette.
Kokkupuuteperiood
Another alternative method could be to produce a sediment slurry and pour this slurry onto a shallow pan of suitable size.
aeg, mille vältel katseorganismid puutuvad kokku uuritava kemikaaliga.
From the shallow layer of slurry the worms can be picked up by a steel needle or watchmakers' tweezers (to be used rather like a fork than forceps to avoid injuring the worms) and transferred to clean water.
Valmistatud sete (ehk taastatud, kunstlik või sünteetiline sete)
After separating the worms from the sediment slurry, these are rinsed in test medium and counted.
selliste materjalide segu, mida kasutatakse loodusliku sette koostisosade imiteerimiseks.
Independently of the method used, laboratories should demonstrate that their personnel are able to recover an average of at least 90 % of the organisms from whole sediment.
Vähim täheldatavat toimet avaldav kontsentratsioon
For example, a certain number of test organisms could be added to control sediment or test sediments, and recovery could be determined after 1 h (7).
uuritava kemikaali väikseim kasutatud kontsentratsioon, mille puhul täheldatakse kemikaali olulist (p ≤ 0,05) mürgist mõju võrdluses kontrolliga.
The total number of living and dead individuals per replicate should be recorded and assessed.
Kemikaal peab kõikidel kõnealusest kontsentratsioonist suurematel kontsentratsioonidel avaldama vähemalt sama tugevat kahjulikku mõju kui kõnealusel kontsentratsioonil avalduv kahjulik mõju.
The following groups of worms are considered to be dead:
Kui nimetatud kahte tingimust ei ole võimalik täita, tuleb lisada ammendav selgitus, kuidas kõnealune kontsentratsioon (ja sellest lähtuvalt ka täheldatavat toimet mitteavaldav kontsentratsioon) on valitud.
there is no reaction after a gentle mechanical stimulus
Täheldatavat toimet mitteavaldav kontsentratsioon
there are signs of decomposition (in combination with “a”)
uuritava kemikaali kontsentratsioon, mille juures ei täheldata kindlaksmääratud kokkupuuteperioodi jooksul kontrolliga võrreldes statistiliselt olulist (p ≤ 0,05) mõju ja mis on kasutatud kontsentratsioonide jadas vahetult allpool vähimat täheldatavat toimet avaldavat kontsentratsiooni.
number of missing worms
Jaotuskoefitsient süsteemis oktanool/vesi (Kow; mõnikord kasutatakse ka tähist Pow)
Additionally, the living worms can be assigned to one of three groups:
n-oktanoolis lahustunud kemikaali ja vees lahustunud kemikaali tasakaalukontsentratsioonide suhe, millega iseloomustatakse kemikaali lipofiilsust (käesoleva lisa peatükk A.24).
large complete worms (adults) without regenerated body regions
Koefitsienti Kow või selle logaritmi (log Kow) kasutatakse näitajana, mis võimaldab hinnata kemikaali veeorganismides bioakumuleerumise tõenäosust.
complete worms with regenerated, lighter-coloured body regions (i.e., with new posterior part, with new anterior part, or with both new posterior and anterior parts)
Jaotuskoefitsient süsteemis orgaaniline süsinik/vesi (Koc) sette orgaanilise süsiniku fraktsioonis ja selle pinnal esineva kemikaali ning vees lahustunud kemikaali tasakaalukontsentratsioonide suhe.
These additional observations are not mandatory, but can be used for additional interpretation of the biological results (for example, a high number of worms assigned to group c may indicate a delay of reproduction or regeneration in a given treatment).
Poorivesi – vesi, mis asub sette- või mullaosakeste vahelises ruumis.
Additionally, if any differences in appearance (e.g. lesions of the integument, oedematous body sections) are observed between treated and control worms, these should be recorded.
Rikastatud sete sete, millele on lisatud uuritavat kemikaali.
Immediately after counting/assessment, the living worms found in each replicate are transferred to dried, pre-weighed and labelled weigh pans (one per replicate), and killed using a drop of ethanol per weigh pan.
a.
The weigh pans are placed in a drying oven at 100 ± 5 °C to dry overnight, after which they are weighed after cooling in a desiccator, and worm dry weight is determined (preferably in g, at least 4 post-decimal digits).
Kaltsiumkloriidi lahus
In addition to the total dry weight, the ash-free dry weight may be determined as described in (49) in order to account for inorganic components originating from ingested sediment present in the alimentary tract of the worms.
Lahustage 11,76 g CaCl2 · 2H2O deioniseeritud vees;
The biomass is determined as total biomass per replicate including adult and young worms.
täiendage sama veega 1 liitrini.
Dead worms should not be taken into account for the determination of biomass per replicate.
b.
Verification of test chemical concentrations
Magneesiumsulfaadi lahus
Sampling
Lahustage 4,93 g MgSO4 · 7H2O deioniseeritud vees;
Samples for chemical analysis of the test chemical should be taken at least of the highest concentration and a lower one, at least at the end of the equilibration phase (before adding the test organisms), and at the end of the test.
c.
At least the bulk sediment and the overlying water should be sampled for analysis.
Naatriumvesinikkarbonaadi lahus
At least two samples should be taken per matrix and treatment on each sampling date.
Lahustage 2,59 g NaHCO3 deioniseeritud vees;
One of the duplicate samples may be stored as a reserve (to be analysed e.g. in the event that initial analysis falls outside the ± 20 % range from the nominal concentration).
d.
In case of specific chemical properties, e.g. if rapid degradation of the test chemical is expected, the analytical schedule may be refined (e.g. more frequent sampling, analysis of more concentration levels) on the basis of expert judgment.
Kaaliumkloriidi lahus
Samples may then be taken on intermediate sampling dates (e.g. on day seven after start of exposure).
Lahustage 0,23 g KCl deioniseeritud vees;
The overlying water should be sampled by carefully decanting or siphoning off the overlying water so as to minimise perturbation of the sediment.
Kõik kemikaalid peavad olema analüüsipuhtad.
The volume of the samples should be recorded.
Destilleeritud või deioniseeritud vee juhtivus ei tohiks ületada 10 μScm– 1.
After the overlying water has been removed, the sediment should be homogenised and transferred to a suitable container.
25 ml iga lahust a kuni d segatakse ja ruumala viiakse deioniseeritud veega 1 liitrini.
The weight of the wet sediment sample is recorded.
Kaltsiumi- ja magneesiumiioonide summaarne sisaldus sellises lahuses on 2,5 mmol/l.
For example, approximately 200 ml of wet sediment can be filled into 250 ml centrifugation beakers.
Selle lahuse puhvermahtuvus happe neutraliseerimisel KS4.3 on 0,8 mmol/l.
Thereafter the samples should be centrifuged without filtration to isolate the porewater, e.g. at 10000 ± 600 × g for 30 - 60 min at a temperature not exceeding the temperature used in the test.
Lahjendusvett aereeritakse, kuni on saavutatud vee küllastumine hapnikuga, seejärel hoitakse seda enne kasutamist umbes kaks päeva ilma edasise aereerimiseta.
After centrifugation, the supernatant is decanted or pipetted taking care that no sediment particles are introduced, and the volume is recorded.
VIIDE
The weight of the remaining sediment pellet is recorded.
Käesoleva lisa peatükk C.1 „Äge mürgisus kaladele”.
It may facilitate the estimation of the mass balance or recovery of the test chemical in the water-sediment system, if the sediment dry weight is determined at each sampling date.
Nõuetekohase lahjendusvee füüsikalis-keemilised omadused
In some cases it might not be possible to analyse concentrations in the pore water as the sample size is too small.
(OECD 1992. aasta juhendi (1) põhjal)
Failing immediate analysis, all samples should be stored by an appropriate method, e.g. under the storage conditions recommended for minimum degradation of the particular test chemical (e.g., environmental samples are commonly stored at – 18 °C in the dark).
Kontsentratsioon
Obtain information on the proper storage conditions for the particular test chemical — for example, duration and temperature of storage, extraction procedures, etc. — before beginning the study.
Tahked osakesed
Analytical method
Orgaanilise süsiniku üldsisaldus
Since the whole procedure is governed essentially by the accuracy, precision and sensitivity of the analytical method used for the test chemical, check experimentally that the precision and reproducibility of the chemical analysis, as well as the recovery of the test chemical from water and sediment samples are satisfactory for the particular method at least at the lowest and highest test concentrations.
< 2 μg/l
Also, check that the test chemical is not detectable in the control chambers in concentrations higher than the limit of quantification.
Ioniseerimata ammoniaak
If necessary, correct the nominal concentrations for the recoveries of quality control spikes (e.g. where recovery is outside 80 - 120 % of spiked amount).
< 1 μg/l
Handle all samples throughout the test in such a manner so as to minimise contamination and loss (e.g. resulting from adsorption of the test chemical on the sampling device).
Jääkkloor
The recovery of test chemical, the limit of quantification, and the limit of detection in sediment and water should be recorded and reported.
< 10 μg/l
The main mandatory response variables of the test to be evaluated statistically are the biomass and the total number of worms per replicate.
Fosfororgaanilised pestitsiidid, üldsisaldus
Optionally, reproduction (as increase of worm numbers) and growth (as increase of dry biomass) could be also evaluated.
< 50 ng/l
In this case, an estimate of the dry weight of the worms at start of exposure should be obtained e.g. by measurement of the dry weight of a representative sub-sample of the batch of synchronised worms to be used for the test.
Kloororgaaniliste pestitsiidide ja polüklooritud bifenüülide üldsisaldus
Although mortality is not an endpoint of this test, mortalities should be evaluated as far as possible.
Orgaanilise kloori üldsisaldus
In order to estimate mortalities, the number of worms that do not react to a gentle mechanical stimulus or showed signs of decomposition, and the missing worms should be considered dead.
< 25 ng/l
Mortalities should at least be recorded and considered when interpreting the test results.
Soovitatav kunstlik sete – valmistamis- ja säilitamisjuhised
Effect concentrations should be expressed in mg/kg sediment dry weight.
Koostisosa
If the recovery of test chemical measured in the sediment, or in sediment and overlying water at start of exposure, is between 80 and 120 % of the nominal concentrations, the effect concentrations (ECx, NOEC, LOEC) may be expressed based on nominal concentrations.
Kirjeldus
If recovery deviates from the nominal concentrations by more than ± 20 % of the nominal concentrations, the effect concentrations (ECx, NOEC, LOEC) should be based on the initially measured concentrations at the beginning of the exposure, e.g. taking into account the mass balance of the test chemical in the test system (see paragraph 30).
Sette kuivmassi protsent
In these cases, additional information can be obtained from analysis of stock and/or application solutions in order to confirm that the test sediments were prepared correctly.
Turvas
ECx-values for the parameters described in paragraph 60 are calculated using appropriate statistical methods (e.g. probit analysis, logistic or Weibull function, trimmed Spearman-Karber method, or simple interpolation).
Turbasamblaturvas, lagunemisaste:
Guidance on statistical evaluation is given in (15) and (50).
„keskmine”, õhu käes kuivatatud, ilma nähtavate taimejäänusteta, peeneks jahvatatud (osakese suurus ≤ 0,5 mm)
To compute the EC50 or any other ECx, the per-treatment means ( X
Kvartsliiv
If a statistical analysis is intended to determine the NOEC/LOEC, per-vessel statistics (individual vessels are considered replicates) are necessary.
≤ 2 mm, kuid > 50 % osakestest peaksid olema vahemikus 50–200 μm
Appropriate statistical methods should be used.
75–76
In general, adverse effects of the test item compared to the control are investigated using one-tailed (smaller) hypothesis testing at p ≤ 0,05.
Kaoliinsavi
Examples are given in the following paragraphs.
Kaoliniidisisaldus ≥ 30 %
Guidance on selection of appropriate statistical methods is given in (15) and (50).
Söödaallikas
Normal distribution of data can be tested e.g. with the Kolmogorov-Smirnov goodness-of-fit test, the Range-to-standard-deviation ratio test (R/s-test) or the Shapiro-Wilk test, (two-sided, p ≤ 0,05).
0,4–0,5 %
Cochran's test, Levene test or Bartlett's test, (two-sided, p ≤ 0,05) may be used to test variance homogeneity.
Orgaaniline süsinik
If the prerequisites of parametric test procedures (normality, variance homogeneity) are fulfilled, One-way Analysis of Variance (ANOVA) and subsequent multi-comparison tests can be performed.
Reguleeritakse turba ja liiva lisamisega
Pairwise comparisons (e.g. Dunnett's t-test) or step-down trend tests (e.g. Williams' test) can be used to calculate whether there are significant differences (p ≤ 0,05) between the controls and the various test item concentrations.
Kaltsiumkarbonaat
Otherwise, non-parametric methods (e.g. Bonferroni-U-test according to Holm or Jonckheere-Terpstra trend test) should be used to determine the NOEC and the LOEC.
CaCO3, pulbriks jahvatatud, keemiliselt puhas, lisaks kuivale settele
If a limit test (comparison of control and one treatment only) has been performed and the prerequisites of parametric test procedures (normality, homogeneity) are fulfilled, metric responses (total worm number, and biomass as worm dry weight) can be evaluated by the Student test (t-test).
Deioniseeritud vesi
The unequal-variance t-test (Welch t-test) or a non parametric test, such as the Mann-Whitney-U-test may be used, if these requirements are not fulfilled.
Juhtivus ≤ 10 μS/cm, lisaks kuivale settele
Some information on the statistical power found during hypothesis testing in the ring test of the method is given in Appendix 6.
30–50
To determine significant differences between the controls (control and solvent control), the replicates of each control can be tested as described for the limit test.
Kui võib eeldada, et ammoniaagisisaldus muutub tavapärasest suuremaks – näiteks kui on teada, et uuritav kemikaal pärsib nitrifitseerimist –, võib olla otstarbekas asendada 50 % lämmastikurikkast nõgeselehtede pulbrist tselluloosiga (nt keemiliselt puhta pulbrilise α-tselluloosiga osakeste suurusega ≤ 0,5 mm (1, 2)).
If these tests do not detect significant differences, all control and solvent control replicates may be pooled.
Valmistamine
Otherwise all treatments should be compared with the solvent control.
Turvas kuivatatakse õhu käes ja jahvatatakse peeneks pulbriks.
The results should be interpreted with caution if there were deviations from this test method, and where measured concentrations of test concentrations occur at levels close to the detection limit of the analytical method used.
Valmistatakse vajaliku turbapulbrikoguse suspensioon deioniseeritud vees, kasutades tõhusat homogenisaatorit.
Any deviations from this test method must be noted.
Selle suspensiooni pH reguleeritakse CaCO3 abil vahemikku 5,5 ± 0,5.
The test report should include at least the following information:
Suspensiooni hoitakse ettevaatlikult segades vähemalt kaks ööpäeva temperatuuril 20 ± 2 °C, et lasta pH-l ja mikroobipopulatsioonidel stabiliseeruda.
chemical identification data (common name, chemical name, structural formula, CAS number, etc.) including purity and analytical method for quantification of test chemical; source of the test chemical, identity and concentration of any solvent used.
Pärast seda määratakse pH uuesti; see peaks olema 6,0 ± 0,5.
any information available on the physical nature and physical-chemical properties as obtained prior to start of the test, (e.g. water solubility, vapour pressure, partition coefficient in soil (or in sediment if available), log Kow, stability in water, etc.);
Seejärel segatakse turba suspensioon muude koostisosadega (liiv ja kaoliinsavi) ning deioniseeritud veega, et saada homogeenne sete, mille veesisaldus on vahemikus 30–50 % sette kuivmassist.
scientific name, source, any pre-treatment, acclimation, culture conditions, etc..
Lõpliku segu pH määratakse uuesti ja see reguleeritakse vajaduse korral CaCO3 abil väärtusele 6,5–7,5.
test procedure used (e.g. static, semi-static or flow-through);
Kui eeldatakse ammoniaagi moodustumist, võib olla kasulik hoida sademe pH allpool 7,0 (nt 6,0 ja 6,5 vahel).
test design (e.g. number, material and size of test chambers, water volume per vessel, sediment mass and volume per vessel, (for flow-through or semi-static procedures: water volume replacement rate), any aeration used before and during the test, number of replicates, number of worms per replicate at start of exposure, number of test concentrations, length of conditioning, equilibration and exposure periods, sampling frequency);
Kuivmassi ja orgaanilise süsiniku sisalduse määramiseks võetakse sette proovid.
depth of sediment and overlying water;
Kui võib eeldada ammoniaagi teket, võib valmistatud setet hoida enne uuritava kemikaali lisamist seitse ööpäeva samades tingimustes, mida kasutatakse järgnevas katses (nt sette ja vee suhe 1:4, settekihi paksus sama kui katsenõudes), st sete tuleks katta veekihiga, mida tuleks aereerida.
method of test chemical pre-treatment and spiking/application;
Konditsioneerimisperioodi lõpus tuleks kattev veekiht eemaldada ja ära visata.
the nominal test concentrations, details about the sampling for chemical analysis, and the analytical methods by which concentrations of the test chemical were obtained;
Seejärel segatakse iga kontsentratsiooni puhul settesse uuritavat kemikaali sisaldav kvartsliiv, sete jaotatakse katses kasutatavatesse paralleelnõudesse ja kaetakse katseveega.
sediment characteristics as described in paragraphs 24 - 25, and any other measurements made;
Nõusid inkubeeritakse seejärel samades tingimustes, mis valdavalt esinevad edasise katse käigus.
preparation of formulated sediment;
See on tasakaalustumisperioodi algus.
preparation of the test water (if reconstituted water is used) and characteristics (oxygen concentration, pH, conductivity, hardness, and any other measurements made) before the start of the test;
Katvat veekihti tuleks aereerida.
methods used for determination of all biological parameters (e.g. sampling, inspection, weighing of test organisms) and all abiotic parameters (e.g. water and sediment quality parameters);
Et tagada uuritava kemikaali segunemine söödaga, tuleks sööt segada settega hiljemalt uuritava kemikaali settele lisamise päeval.
volumes and/or weights of all samples for chemical analysis;
Säilitamine
detailed information on the treatment of all samples for chemical analysis, including details of preparation, storage, spiking procedures, extraction, and analytical procedures (and precision) for the test chemical, and recoveries of the test chemical.
Kunstliku sette kuivi koostisosi võib säilitada kuivas ja jahedas kohas või toatemperatuuril.
water quality within the test vessels (pH, temperature, dissolved oxygen concentration, hardness, ammonia concentrations, and any other measurements made);
Uuritavat kemikaali sisaldav valmistatud sete tuleks katses viivitamata ära kasutada.
total organic carbon content (TOC), dry weight to wet weight ratio, pH of the sediment, and any other measurements made;
Rikastatud mulla proove võib nende analüüsimiseni säilitada konkreetse uuritava kemikaali jaoks soovitatavates tingimustes.
total number, and if determined, number of complete and incomplete worms in each test chamber at the end of the test;
Lumbriculus variegatus'e kultiveerimise meetodid
dry weight of the worms of each test chamber at the end of the test, and if measured, dry weight of a sub-sample of the worms at start of the test;
Kultuuris pidamise meetodid
any observed abnormal behaviour in comparison to the controls (e.g., sediment avoidance, presence or absence of fecal pellets);
Kultiveerimismeetodite ülevaade on esitatud allpool.
any observed mortalities;
Lühike kokkuvõte neist on esitatud allpool.
estimates of toxic endpoints (e.g. ECx, NOEC and/or LOEC), and the statistical methods used for their determination;
L. variegatus'e kasutamise oluline eelis on selle liigi isendite kiire paljunemine ja sellest tulenev kiire biomassi suurenemine laboris kultiveeritavates populatsioonides (nt allikad 1, 3, 4, 5).
the nominal test concentrations, the measured test concentrations and the results of all analyses made to determine the concentration of the test chemical in the test vessels;
Usse võib kasvatada suurtes akvaariumides (mahuga 57–80 l) temperatuuril 23 °C valgustusperioodiga 16 tundi valgust (100–1000 luksi) ja 8 tundi pimedust, kasutades looduslikku vett, mida uuendatakse iga päev (45–50 l akvaariumi kohta).
any deviations from the validity criteria.
Substraat valmistatakse pleegitamata pruunide paberrätikute lõikamisega ribadeks, mida võib seejärel mõne sekundi jooksul segada kultuuris kasutatava veega, et saada väiksed pabersubstraadi tükid.
Evaluation of results:
Seda substraati saab otse kasutada rabeliimuka kasvatamise akvaariumis, kui laotada see nõu põhjale, või seda võib säilitada külmutatult deioniseeritud vees hilisemaks kasutamiseks.
compliance of the results with the validity criteria as listed in paragraph 13;
Üldjuhul jagub uuest substraadist akvaariumis umbes kaheks kuuks.
Chapter C.27 of this Annex, “Sediment-water chironomid toxicity test using spiked sediment”.
Iga ussikultuuri loomist alustatakse 500–1000 ussiga, keda söödetakse perioodiliselt uuendatavas vees või läbivoolurežiimis 3 korda nädalas 10 ml suspensiooniga, mis sisaldab 6 g forelli noorkalade sööta.
Validation of a sediment toxicity test with the endobenthic aquatic oligochaete Lumbriculus variegatus by an international ring test.
Staatilises või poolstaatilises kultuuris tuleks bakterite ja seente kasvu ärahoidmiseks söötmissagedust vähendada.
202 67 429.
Nendes tingimustes kahekordistub isendite arv kultuuris üldjuhul umbes 10–14 päeva jooksul.
Report EPS 1/RM/___
Teise võimalusena võib Lumbriculus variegatus't kultiveerida süsteemis, mis koosneb kunstliku sette valmistamisel kasutatava kvartsliiva kihist (paksus 1–2 cm) ja taastatud veest.
Lumbriculus variegatus, a Benthic Oligochaete, as a Bioassay Organism. p.
Kultuuri saab pidada klaasist või roostevabast terasest mahutites, mille kõrgus on 12–20 cm.
205-215.
Vett nõudes tuleks kergelt aereerida (nt 2–4 mulli sekundis), kasutades Pasteuri pipetti, mis jääb settepinnast umbes 2 cm kõrgusele.
Chapter C.1 of this Annex, Fish, Acute Toxicity Test.
Et hoida ära näiteks ammoniaagi akumuleerumist, tuleks sette kohal oleva vee uuendamiseks kasutada läbivoolusüsteemi või uuendada vett käsitsi vähemalt kord nädalas.
Workshop on “Bioaccumulation: Sediment test using benthic oligochaetes”, 26.-27.4.1999, Hochheim/Main, Germany.
Väheharjasusse võib kasvatada toatemperatuuril valgustusperioodiga 16 tundi valgust (valgustustihedus 100–1000 luksi) ja 8 tundi pimedust.