natgillin/translations-raw huggingface dataset:
rationale for selection of concentrations of the test chemical used in the presence and in the absence of irradiation,
kiiritatud ja kiiritamata proovides kasutatud uuritava kemikaali kontsentratsioonide valiku põhjendus;
in case of limited solubility of the test chemical and absence of cytotoxicity: rationale for the highest concentration tested,
uuritava kemikaali piiratud lahustuvuse ja tsütotoksilisuse puudumise puhul: kõrgeima uuritud kontsentratsiooni valiku põhjendus;
type and composition of treatment medium (buffered salt solution),
töötlemiskeskkonna tüüp ja koostis (soolasisaldusega puhverlahus);
duration of the chemical treatment.
kemikaaliga töötlemise kestus.
Test conditions (3); irradiation:
Katse tingimused (3): kiiritamine:
rationale for selection of the light source used,
kasutatud valgusallika – valiku põhjendus;
manufacturer and type of light source and radiometer,
valgusallika ja kiirgusmõõtja valmistaja ja tüüp;
spectral irradiance characteristics of the light source,
valgusallika kiirguse spektri näitajad;
transmission and absorption characteristics of the filter(s) used,
kasutatud filtri(te) läbilaskvuse ja neeldumise näitajad;
characteristics of the radiometer and details on its calibration,
kiirgusmõõtja iseloomustus ja kaliibrimise üksikasjad;
distance of the light source from the test system,
valgusallika kaugus katsesüsteemist;
UVA irradiance at this distance, expressed in mW/cm2,
UVA-kiirgus kõnealuse kauguse puhul, väljendatuna mW/cm2;
duration of the UV/vis light exposure,
UV/nähtava valgusega kiiritamise kestus;
UVA dose (irradiance × time), expressed in J/cm2,
UVA-annus (kiiritustihedus × aeg), väljendatuna J/cm2;
temperature of cell cultures during irradiation and cell cultures concurrently kept in the dark.
rakukultuuride temperatuur kiiritamise ajal ja samaaegselt pimedas hoitud rakukultuuride temperatuur.
Test conditions (4); Neutral Red viability test:
Katse tingimused (4): elujõulisuse määramine neutraalpunase abil:
composition of Neutral Red treatment medium,
neutraalpunasega töötlemise keskkonna koostis;
duration of Neutral Red incubation,
neutraalpunasega inkubeerimise kestus;
Neutral Red extraction conditions (extractant; duration),
neutraalpunase ekstraheerimise tingimused (ekstrahent; kestus);
wavelength used for spectrophotometric reading of Neutral Red optical density,
neutraalpunase optilise tiheduse spektrofotomeetriliseks määramiseks kasutatud lainepikkus;
second wavelength (reference), if used,
teine (võrdlus)lainepikkus, kui seda kasutati;
content of spectrophotometer blank, if used.
mida kasutati spektrofotomeetrilise pimekatsena (kui kasutati).
cell viability obtained at each concentration of the test chemical, expressed in percent viability of mean, concurrent solvent controls,
rakkude mõõdetud elujõulisus uuritava kemikaali igal kontsentratsioonil, võrreldes samaaegsete lahustikontrollproovide keskmise elujõulisusega, protsentides;
concentration response curves (test chemical concentration vs. relative cell viability) obtained in concurrent +Irr and -Irr experiments,
kontsentratsiooni-mõju sõltuvused (rakkude suhtelise elujõulisuse sõltuvus uuritava kemikaali kontsentratsioonist), mis on saadud samaaegsete +Irr- ja –Irr-katsetega;
analysis of the concentration-response curves: if possible, computation/calculation of IC50 (+Irr) and IC50 (-Irr),
kontsentratsiooni-mõju sõltuvuste analüüs: võimaluse korral IC50 (+Irr) ja IC50 (–Irr) arvutamine;
comparison of the two concentration response curves obtained in the presence and in the absence of irradiation, either by calculation of the Photo-Irritation-Factor (PIF), or by calculation of the Mean-Photo-Effect (MPE),
kiiritatud ja kiiritamata proovidest mõõdetud kahe kontsentratsiooni-mõju sõltuvuse võrdlus kas fotoärrituvusteguri (PIF) või keskmise fotoefekti (MPE) arvutamisega;
test acceptance criteria; concurrent solvent control:
katse nõuetekohasuse tingimused; samaaegsed lahustikontrollproovid:
absolute viability (optical density of Neutral Red extract) of irradiated and non-irradiated cells,
kiiritatud ja kiiritamata rakkude absoluutne elujõulisus (neutraalpunase ekstrakti optiline tihedus);
historic negative and solvent control data; means and standard deviations,
varasemad negatiivsete ja lahustikontrollproovide andmed; keskväärtused ja standardhälbed;
test acceptance criteria; concurrent positive control,
katse nõuetekohasuse tingimused; samaaegne positiivne kontrollproov;
IC50(+Irr) and IC50(-Irr) and PIF/MPE of positive control chemical,
positiivse kontrollina kasutatud kemikaali IC50 (+Irr) ja IC50 (–Irr) ning PIF/MPE;
historic positive control chemical data: IC50(+Irr) and IC50(-Irr) and PIF/MPE; means and standard deviations.
varasemad andmed positiivse kontrolli kemikaali kohta: IC50 (+Irr) ja IC50 (–Irr) ja PIF/MPE; keskväärtused ja standardhälbed.
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http://ww.oecd.org/document/55/0,2340,en_2649_34377_2349687_l_l_l_l,00.html
Role of the 3T3 NRU PT in a sequential approach to the phototoxicity testing of chemicals
3T3 NRU PT osa kemikaalide fototoksilisuse uuringu jadakäsitlusel
Initial Evaluation of the Physical, Chemical, and Toxicological Properties of the Test Substance
Uuritava aine füüsikaliste, keemiliste ja toksikoloogiliste omaduste esialgne hindamine
Physico-chemical properties
• Füüsikalis-keemilised omadused
Chemical structure, structural alerts
• Keemiline struktuur, struktuursed teated
UV/vis - absorption
• UV-/nähtav piirkond - neeldumine
QSAR - photochemistry
• QSAR - fotokeemia
General toxicity (including kinetics and metabolism)
• Üldine toksilisus (sh kineetika ja metabolism)
UV/vis absorption spectra in appropriate solvent (e.g. OECD TG 101)
UV-/nähtava piirkonna neeldumisspekter sobivas lahustis nt OECD TG 101)
No Absorption
Neeldumine puudub
Phototoxicity Testing not considered necessary
Fototoksilisuse uurimist ei peeta vajalikuks
In Vitro 3T3 NRU Phototoxicity Test and/or other methods if necessary
In vitro 3T3 NRU fototoksilisuse katse ja/või muud meetodid (vajadusel)
Spectral power distribution of a filtered solar simulator
„Kunstliku päikese” filtritud kiirgusvoo spektraaljaotus
SOL 500 + H2 Filter
SOL 500 + filter H2
SOL 500 + H1 Filter
SOL 500 + filter H1
SOL 500 + H1 Filter + lid of 96-well plate
SOL 500 + filter H1 + 96 süvendiga plaadi kaas
(see Section 1.4.1.5, second paragraph)
(vt punkti 1.4.1.5 teist lõiku)
Figure 1 gives an example of an acceptable spectral irradiance distribution of a filtered solar simulator.
Joonisel 1 on esitatud näide, milline peaks olema „kunstliku päikese” flltritud kiirgusvoo spektraaljaotus.
It is from the doped metal halide source used in the validation trial of the 3T3 NRU PT (6)(8)(17).
Valgusallikas on dopeeritud metallhalogeniidkaarlamp, mida kasutati 3T3 NRU fototoksilisuse määramise valideerimisel (6, 8, 17).
The effect of two different filters and the additional filtering effect of the lid of a 96-well cell culture plate are shown.
Joonisel on näidatud kahe erineva filtri toime ja 96 süvendiga rakukultuuri plaadi kaane täiendav filtriv toime.
The H2 filter was only used with test systems that can tolerate a higher amount of UVB (skin model test and red blood cell photo-haemolysis test).
Filtrit H2 kasutati ainult katsesüsteemide puhul, mis taluvad suuremat hulka UVB kiirgust (nahamudeli test ja vere punaliblede foto-hemolüüsi test).
In the 3T3 NRU-PT the H1 filter was used.
3T3 NRU fototoksilisuse katses kasutati filtrit H1.
The figure shows that additional filtering effect of the plate lid is mainly observed in the UVB range, still leaving enough UVB in the irradiation spectrum to excite chemicals typically absorbing in the UVB range, like Amiodarone (see Table 1).
Jooniselt on näha, et plaadi kaane täiendav filtriv toime ilmneb peamiselt UVB kiirguse alas; siiski jääb kiirgusspektrisse piisavalt UVB kiirgust, et ergastada selliseid kemikaale nagu amiodaroon, mille neeldumisspekter asub tavaliselt UVB kiirguse piirkonnas (vt tabelit 1).
Irradiation sensivity of Balb/c 3T3 cells (as measured in the UVA range)
Balb/c 3T3 rakkude kiirgustundlikkus (mõõdetud UVA kiirguse alas)
Cell viability (% Neutral Red uptake of dark controls)
Rakkude elujõulisus (neutraalpunase sidumise protsent kiiritamata kontrollproovides)
irradiation time (minutes)
kiiritamise aeg (minutites)
10 min = 1 joule UVA/cm2)
(10 min = 1 džaul UVA/cm2)
(see Sections 1.4.1.5.2 second paragraph; 1.4.2.2.1, 1.4.2.2.2)
(vt punkti 1.4.1.5.2 teist lõiku ja punkte 1.4.2.2.1, 1.4.2.2.2)
Sensitivity of Balb/c 3T3 cells to irradiation with the solar simulator used in the validation trial of the 3T3NRU-phototoxicity test, as measured in the UVA range.
Balb/c 3T3 rakkude tundlikkus 3T3 NRU fototoksilisuse katse valideerimisel kasutatud „kunstliku päikesega” kiiritamise suhtes, mõõdetuna UVA piirkonnas.
Figure shows the results obtained in seven different laboratories in the pre-validation study (1).
Joonisel on näidatud seitsmes laboris saadud valideerimiseelsed tulemused (1).
While the two curves with open symbols were obtained with aged cells (high number of passages), that had to be replaced by new cell stocks the curves with bold symbols show cells with acceptable irradiation tolerance.
Kaks valgete sümbolitega sõltuvust saadi vananenud rakkudega (palju passaaže), need rakud tuli asendada uute varurakkudega; mustade sümbolitega sõltuvused iseloomustavad rakke, kelle kiirgustaluvus on lubatud piirides.
From these data the highest non-cytotoxic irradiation dose of 5 J/cm2 was derived (vertical dashed line).
Nendest andmetest määrati kõrgeim mittetsütotoksiline kiirgusdoos 5 J/cm2.
The horizontal dashed line shows in addition the maximum acceptable irradiation effect given in paragraph 1.4.2.2.
Vertikaalne katkendjoon näitab lisaks maksimaalset kiirgustaluvust, mida käsitletakse punktis 1.4.2.2.
This test method is equivalent to the OECD TG 429 (2002)
Käesolev katsemeetod on samaväärne OECD TG 429ga (2002).
The Local Lymph Node Assay (LLNA) has been sufficiently validated and accepted to justify its adoption as a new Method (1)(2)(3).
Paikne lümfisõlmede uuring (Local Lymph Node Assay, LLNA) on piisavalt valideeritud ja heaks kiidetud, et selle võiks põhjendatult võtta vastu uue meetodina (1, 2, 3).
This is the second method for assessing skin sensitisation potential of chemicals in animals.
See on teine meetod kemikaalide nahatundlikkusvõime hindamiseks loomadel.
The other method (B.6) utilises guinea pig tests, notably the guinea pig maximisation test and the Buehler test (4).
Teises meetodis (B.6) kasutatakse katseid merisigadega, nimelt merisigadega tehtav maksimiseerimiskatse ja Buehleri katse (4).
The LLNA provides an alternative method for identifying skin sensitising chemicals and for confirming that chemicals lack a significant potential to cause skin sensitisation.
LLNA on alternatiivne meetod, mille abil võidakse kindlaks teha nahka sensibiliseerivad kemikaalid ja kinnitada, et kemikaalidel puudub oluline naha sensibiliseerimisvõime.
This does not necessarily imply that in all instances the LLNA should be used in place of guinea pig test, but rather that the assay is of equal merit and may be employed as an alternative in which positive and negative results generally no longer require further confirmation.
See ei tähenda tingimata seda, et kõikidel juhtudel tuleks kasutada LLNA merisigadega tehtavate katsete asemel, kuid pigem seda, et uuring on ühtmoodi hea ja seda võib kasutada alternatiivina, milles positiivsed ja negatiivsed tulemused üldiselt ei vaja enam kinnitamist.
The LLNA provides certain advantages with regard to both scientific progress and animal welfare.
LLNA näeb ette teatavad soodustused seoses nii teadusliku arengu kui ka loomade heaoluga.
It studies the induction phase of skin sensitisation and provides quantitative data suitable for dose response assessment.
See uurib nahatundlikkuse esilekutsumise etappi ja esitab kvantitatiivsed andmed, mis sobivad annusele reageerimise hindamiseks.
The details of the validation of the LLNA and a review of the associated work have been published (5)(6)(7)(8).
LLNA valideerimise üksikasjad ja sellega seotud töö ülevaade avaldatakse (5, 6, 7, 8).
In addition, it should be noted that the mild/moderate sensitisers, which are recommended as suitable positive control substances for guinea pig test m, are also appropriate for use with the LLNA (6)(8)(9).
Lisaks sellele tuleks märkida, et kerged/mõõdukad sensibilisaatorid, mida soovitatakse sobivaks positiivseks kontrollaineks merisigadega tehtavate katsemeetodite puhul, on samuti sobivad kasutamiseks LLNAga (6, 8, 9).
The LLNA is an in vivo method and, as a consequence, will not eliminate the use of animals in the assessment of contact sensitising activity.
LLNA on in vivo meetod ja seetõttu ei lõpetata loomade kasutamist puutetundlikkuse hindamisel.
It has, however, the potential to reduce the number of animals required for this purpose.
Selles võidakse siiski vähendada selleks ettenähtud loomade arvu vähendamist.
Moreover, the LLNA offers a substantial refinement of the way in which animals are used for contact sensitisation testing.
Lisaks sellele LLNA-s kasutatud viis loomade puutetundlikkuse uurimiseks on tunduvalt täiustatum.
The LLNA is based upon consideration of immunological events stimulated by chemicals during the induction phase of sensitisation.
LLNA põhineb kemikaalide põhjustatud immunoloogiliste juhtumite kontrollil tundlikkuse esilekutsumisel.
Unlike guinea pig tests the LLNA does not require that challenged-induced dermal hypersensitivity reactions be elicited.
Erinevalt merisigadega tehtavatest katsetest ei eelda LLNA, et tekiksid esilekutsutud naha ülitundlikkusreaktsioonid.
Furthermore, the LLNA does not require the use of an adjuvant, as is the case for the guinea pig maximisation test.
Samuti ei eelda LLNA adjuvandi kasutamist, nagu seda tehakse merisigadega tehtava maksimeerimiskatse puhul.
Thus, the LLNA reduces animal distress.
Seetõttu vähendab LLNA loomade kannatusi.
Despite the advantages of the LLNA over traditional guinea pig tests, it should be recognised that there are certain limitations that may necessitate the use of traditional guinea pigs tests (e.g. false negative findings in the LLNA with certain metals, false positive findings with certain skin irritants)(10).
Hoolimata LLNA hüvedest traditsiooniliste meriseakatsetega võrreldes, tuleks tunnistada, et on teatud piirangud, mis võivad tingida traditsiooniliste meriseakatsete kasutamise (nt valenegatiivsed LLNA leiud teatavate metallide puhul, valepositiivsed leiud teatavate nahka ärritavate ainete puhul) (10).
See also Introduction part B.
Vt ka B osa sissejuhatust.
The basic principle underlying the LLNA is that sensitisers induce a primary proliferation of lymphocytes in the lymph node draining the site of chemical application.
LLNA põhimõte on selline, et sensibilisaatorid kutsuvad esile primaarse lümfotsüütide vohamise lümfisõlmes, mis tühjendab keemilise aine ala.
This proliferation is proportional to the dose applied (and to the potency of the allergen) and provides a simple means of obtaining an objective, quantitative measurement of sensitisation.
Nimetatud vohamine on võrdeline manustatud annusega (ja allergeeni tõhususega) ning pakub lihtsaid vahendeid tundlikkuse objektiivseks ja kvantitatiivseks mõõtmiseks.
The LLNA assesses this proliferation as a dose-response relationship in which the proliferation in test groups is compared to that in vehicle treated controls.
LLNA hindab nimetatud vohamist annuse ja sellele reageerimise suhtena, milles vohamist katserühmades võrreldakse vohamisega kandeainet manustatud kontrollrühmades.
The ratio of the proliferation in treated groups to that in vehicular controls, termed the Stimulation Index, is determined, and must be at least three before a test substance can be further evaluated as a potential skin sensitiser.
Katserühmade ja kandeainet saanud kontrollrühmade vohamise suhe (stimulatsiooniindeks) määratakse kindlaks ja see peab olema vähemalt 3 enne, kui katseainet saab edasi hinnata võimaliku nahasensibilisaatorina.
The methods described here are based on the use of radioactive labelling to measure cell proliferation.
Siin kirjeldatud meetodid põhinevad radioaktiivse märgistuse kasutamisel, et mõõta raku vohamist.