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Οι οργανισμοί δοκιμής εκτίθενται στην ελεγχόμενη χημική ουσία κατά τη φάση πρόσληψης, η οποία θα πρέπει να διαρκεί 14 ημέρες (Enchytraeidae) ή 21 ημέρες (γαιοσκώληκες), εκτός εάν καταδεικνύεται ότι έχει επιτευχθεί σταθερή κατάσταση.
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The maximum water holding capacity (WHC) of the artificial soil is determined according to ISO 11268-2 (35). At least two days before starting the test, the dry artificial soil is moistened by adding enough deionised or reconstituted water to obtain approximately half of the final water content.
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Η συσκευή που χρησιμοποιείται είναι μια μικροστήλη (σχήμα 1) η οποία διατηρείται σε σταθερή θερμοκρασία και είναι συνδεδεμένη με αντλία ανακυκλοφορίας (σχήμα 2) ή με δοχείο ισοστάθμισης (σχήμα 3).
The apparatus consists of a microcolumn (Figure 1), maintained at constant temperature. It is connected either to a recirculating pump (Figure 2) or to a levelling vessel (Figure 3).
Κατ’ αναλογία, υπολογίζεται η σταθμισμένη τυπική απόκλιση, η οποία χαρακτηρίζει την επαναληψιμότητα της μέτρησης του POW.
Analogously, the weighted standard deviation is calculated. It characterizes the repeatability of the POW measurement.
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Σε περίπτωση που ο φορέας δεν είναι το νερό, πρέπει να είναι γνωστά τα τοξικά χαρακτηριστικά του και να προσδιορίζεται η σταθερότητα της ελεγχόμενης χημικής ουσίας στον φορέα.
For vehicles other than water the toxic characteristics of the vehicle must be known. The stability of the test chemical in the vehicle should be determined.
Όταν η ελεγχόμενη χημική ουσία χορηγείται με καθετήρα, η χορήγηση πρέπει να γίνεται εφάπαξ με τη βοήθεια στομαχικού καθετήρα ή κατάλληλης διασωλήνωσης.
When the test chemical is administered by gavage, this should be done in a single dose to the animals using a stomach tube or a suitable intubation cannula. The maximum volume of liquid that can be administered at one time depends on the size of the test animal.
Οι παρατηρήσεις αυτές πρέπει να γίνονται, κατά προτίμηση, την ίδια ώρα της ημέρας, σε τυποποιημένο χώρο έξω από τους κλωβούς κάθε φορά, και να καταγράφονται προσεκτικά, κατά προτίμηση με τη χρήση συστημάτων βαθμολόγησης τα οποία καθορίζει λεπτομερώς το εργαστήριο.
These observations should be made outside the home cage in a standard arena and preferably at the same time of day on each occasion. They should be carefully recorded, preferably using scoring systems, explicitly defined by the testing laboratory.
Η φύλαξη δειγμάτων πλάσματος ή ορού για τη μέτρηση των θυρεοειδικών ορμονών (Τ3, Τ4) και της θυρεοτροπίνης (προαιρετικά) ενδέχεται να αποδειχθεί χρήσιμη, αν υπάρχουν ενδείξεις επίδρασης στον άξονα υπόφυσης-θυρεοειδούς, παρά το γεγονός ότι δεν κατέστη δυνατόν να αποδειχθεί κατά τη διεθνή αξιολόγηση των σχετικών με το ενδοκρινικό σύστημα καταληκτικών σημείων αν ο προσδιορισμός των ορμονών Τ3, Τ4 και TSH παρέχει σαφές πλεονέκτημα Τα δείγματα αυτά ψύχονται στους – 20° για αποθήκευση.
Although in the international evaluation of the endocrine related endpoints a clear advantage for the determination of thyroid hormones (T3, T4) and TSH could not be demonstrated, it may be helpful to retain plasma or serum samples to measure T3, T4 and TSH (optional) if there is an indication for an effect on the pituitary-thyroid axis. These samples may be frozen at – 20° for storage.
Με τη σύγκριση των επιδράσεων σε ένα εύρος δόσεων αποφεύγεται η χρήση πολλών δοκιμών t. Οι στατιστικές μέθοδοι πρέπει να επιλέγονται κατά τον σχεδιασμό της μελέτης.
Comparisons of the effect along a dose range should avoid the use of multiple t-tests. The statistical methods should be selected during the design of the study.
Οιστρογονικότητα είναι η ικανότητα μιας χημικής ουσίας να δρα όπως μια φυσική οιστρογόνος ορμόνη (π.χ. 17β-οιστραδιόλη) στον οργανισμό θηλαστικού.
Oestrogenicity is the capability of a chemical to act like a natural oestrogenic hormone (e.g. oestradiol 17ß) in a mammalian organism. Test chemical: Any substance or mixture tested using this Test Method. Thyroid activity is the capability of a chemical to act like a natural thyroid hormone (e.g. T3) in a mammalian organism. Validation is a scientific process designed to characterise the operational requirements and limitations of a test method and to demonstrate its reliability and relevance for a particular purpose.
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Η διάρκεια μιας έκθεσης καλύπτει τον χρόνο παραγωγής της ελεγχόμενης χημικής ουσίας, στον οποίο λαμβάνονται υπόψη οι χρόνοι που απαιτούνται για την εξισορρόπηση του θαλάμου (t95) και τη διάσπαση.
The duration of an exposure spans the time that the test chemical is generated. This takes into account the times required to attain chamber equilibration (t95) and decay.
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