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0,4-0,5 (4, 14, 19)
Rodriguez, P. & Reynoldson, T.B.
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Liebig, M., Egeler, Ph.
41 -56 (19, 29)
Oehlmann, J., & Knacker, Th.
0,8 — 1,5 (14, 19)
Bioaccumulation of14C-17α-ethinylestradiol by the oligochaete Lumbriculus variegatus in artificial sediment.
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Rozpusťte 11,76 g CaCl2 · 2H2O v deionizované vodě a doplňte na objem 1 l deionizovanou vodou.
Morton, S.
Rozpusťte 4,93 g MgSO4 · 7H2O v deionizované vodě a doplňte na objem 1 l deionizovanou vodou.
(Herbiseed).
Rozpusťte 2,59 g NaHCO3 v deionizované vodě a doplňte na objem 1 l deionizovanou vodou.
USDA, NRCS.
Rozpusťte 0,23 g KCl v deionizované vodě a doplňte na objem 1 l deionizovanou vodou.
National Plant Data Centre, Baton Rouge, LA 70874-4490 USA
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USEPA.
Např. list kopřivy v prášku (Folia urticae), listy Urtica dioica (kopřiva dvoudomá), jemně mleté (velikost částic ≤ 0,5 mm), v souladu s lékárenskými normami, pro lidskou spotřebu;
Webster, R.H.
Očekávají-li se zvýšené koncentrace amoniaku, např. je-li známo, že zkoušená chemická látka inhibuje nitrifikaci, může být vhodné nahradit 50 % kopřivového prášku bohatého na dusík celulózou (např. práškem α-celulózy, chemicky čistým, s velikostí částic ≤ 0,5 mm; (1) (2)).
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350 ± 50 ppm;
I-20047 Brugherio (MB) Itálie
The vehicle is sprayed on or mixed with a small amount, for example 2,5 g, of fine quartz sand.
MDD (minimum detectable difference):
Alternatively, a multiple U-test, e.g. the Bonferroni-U-test according to Holm (29), or when these data exhibit heteroscedasticity but are otherwise consistent with a underlying monotone dose-response, an other non-parametric test [e.g. Jonckheere-Terpstra (30) (31) or Shirley (32) (33)] can be applied and would generally be preferred to unequal-variance t-tests. (see also the scheme in Appendix 7).
Reprodukční schopnost roztočů vystavených zkoušené chemické látce se srovná se schopností kontrolní skupiny a stanoví se ECx (např. EC10, EC50) nebo koncentrace bez pozorovaných účinků (NOEC) (definice – viz dodatek 1) v závislosti na experimentálním uspořádání (viz odstavec 29).
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Přehled časového sledu zkoušky je uveden v dodatku 8.
Entwicklung eines Reproduktionstests an Bodenorganismen — Enchytraeen.
Nosič se nastříkne na malé množství, například 10 g, jemného křemenného písku, nebo se s ním smíchá.
Römbke, J. and Moser, T. (1999).
Ve zvláštních případech (např. když je stárnutí považováno za určující faktor) je možné čas mezi přípravou konečného zkušebního substrátu a přidáním roztočů prodloužit (podrobnosti takového stárnutí viz (18)).
Westheide, W. and Bethge-Beilfuss, D. (1991).
Avšak v takových případech musí být uvedeno vědecké odůvodnění.
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Je třeba použít vhodné statistické metody (podle dokumentu OECD č. 54 – Současné přístupy ve statistické analýze údajů o ekotoxicitě: Pokyny pro aplikaci).
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No success
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Yes
Doba vývoje od vajíčka k dospělé samičce je přibližně 20 dní při 20 °C.
Parametric Tests
Předem je třeba udržovat a přechovávat více než jednu kulturu.
log Kow;
Chov druhu Typrophagus putrescentiae:
Since a single summary statistic cannot be prescribed for the test, this test method makes provision for the determination of the NOEC and the ECx.
Roztoči jsou přechováváni ve skleněné nádobě naplněné jemným práškem z pivovarských kvasnic, která je umístěna v plastovém kbelíku naplněném roztokem KNO3, aby se zabránilo jejich úniku.
A NOEC is likely to be required by regulatory authorities for the foreseeable future.
Potravinoví roztoči se umístí na povrch tohoto prášku.
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Esterases of earthworm — III.
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Přístroj:
SETAC (1998).
Tullgrenova nálevka nebo srovnatelné metody, např. McFadyenova (vrchní zahřívání, vzorek je vkládán přes nálevku).
EPA (1996).
Režim zahřívání:
Bouché, M.B.
25 °C po dobu 12 h, 35 °C po dobu 12 h, 45 °C po dobu 24 hodin (celkem 48 h).
Edwards, C.A.
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Fixační kapalina:
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Ze skleněné lahvičky použité pro zkoušku se sundá víčko a okolo hrdla se ováže kus síťoviny nebo tkaniny.
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Z tohoto důvodu je kutikula kořisti proražena chelicerami a do jejího těla je vypuštěn sekret pro mimostřevní trávení.
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60, ENV/JM/MONO(2006)27.
Earlier work in the UK (6)(7) described a manometric method using undiluted digesting sludge, plus raw sewage sludge as the substrate, in 500 ml flasks; the apparatus was cumbersome and the stench of the raw sludge was offensive.
OECD (2006b) Report of the Initial Work Towards the Validation of the 21-Day Fish Screening Assay for the Detection of Endocrine active Substances (Phase 1B).
Later the more compact and convenient apparatus of Shelton and Tiedje (8) as developed by Battersby and Wilson (9) was successfully applied by Wilson et al.
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61, ENV/JM/MONO(2006)29.
Glass serum bottles of nominal volume 125 ml, with an actual volume of around 160 ml, sealed with serum septa [5] and crimped aluminium rings are recommended; but bottles of total volume between 0,1 and 1 litre may be used successfully;
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micro syringes, for the gas-tight connection of the pressure transducer (see paragraph 12(c)) to the headspace in the bottles (see paragraph 12(b)); also for adding insoluble liquid test materials into the bottles;
Phase 2: Testing Negative Substances.
P c
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76(3-4), pp.
Using a 250 μm mesh will prevent the worms from crawling into or through the mesh; however, care should be taken that as little as possible of the sediment particles is retained on the mesh.
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chemical identification data (common name, chemical name, structural formula, CAS number, etc.) including purity and analytical method for quantification of test chemical;
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New tables for multiple comparisons with a control.
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Dissolve 11,76 g CaCl2·2H2O in deionised water; make up to 1 l with deionised water
Brion, F., Nilsen, B.
Dissolve 4,93 g MgSO4·7H2O in deionised water; make up to 1 l with deionised water
M., Eidem, J.
Dissolve 2,59 g NaHCO3 in deionised water; make up to 1 l with deionised water
K., Goksoyr, A., Porcher, J.
Dissolve 0,23 g KCl in deionised water; make up to 1 l with deionised water
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e.g. Urtica powder (Folia urticae), leaves of Urtica dioica (stinging nettle), finely ground (particle size ≤ 0,5 mm); in accordance with pharmacy standards, for human consumption;
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If elevated ammonia concentrations are expected, e.g. if the test chemical is known to inhibit nitrification, it may be useful to replace 50 % of the nitrogen-rich urtica powder by cellulose (e.g., α-Cellulose powder, chemically pure, particle size ≤ 0,5 mm; (1) (2)).
1699–1708.
Preparation
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The reproductive output of the mites exposed to the test chemical is compared to that of the controls in order to determine the ECx (e.g. EC10, EC50) or the no observed effect concentration (NOEC) (see Appendix 1 for definitions), depending on the experimental design (see paragraph 29).
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An overview of the test schedule is given in Appendix 8.
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The vehicle is sprayed on or mixed with a small amount, for example 10 g, of fine quartz sand.
301–308.
In specific cases (e.g. when ageing is considered to be a determining factor), the time between preparation of the final test substrate and the addition of the mites can be prolonged (for details of such ageing, see (18)).
Ankley, G.
However, in such cases a scientific justification must be provided.
T., Jensen, K.
Appropriate statistical methods should be used (according to OECD Document 54 on the Current Approaches in the Statistical Analysis of Ecotoxicity Data: A Guidance to Application).