the Sanger method was the use of dideoxynucleotide triphosphates (ddNPTs) as DNA chain terminators. The classical chain-termination method requires a single-stranded DNA template, a DNA primer , a DNA polymerase , normal deoxynucleotidephosphates (dNTPs), and modified nucleotides (dideoxyNTPs) that terminate DNA strand elongation. These ddNTPs will also be radioactively or fluorescently