mwirth-epo/epo-nmt-datasets huggingface dataset:
锁紧螺栓可适于沿基本线性路径行进。
The locking bolt may be adapted to travel along a substantially linear pathway.
圆盘的旋转可适于可往复地将铰接臂以及螺栓和封闭件缩回打开位置，或将铰接臂以及锁紧螺栓和封闭件延伸至关闭位置。
Rotation of the disc may be adapted to reciprocatably retract the articulated arm, and hence the bolt and closure, into an open position, or to extend the articulated arm, and hence the locking bolt and closure, into a closed position.
该螺栓可以包括空心轴。
The bolt may comprise a hollow shaft.
螺栓可包括纵向对齐或螺旋筋，进行加固和加强。
The bolt may include longitudinally aligned or spiral ribs for reinforcement and strengthening.
流下的水drb被作为蒸发器发挥功能的下侧热交换器5A和在下侧热交换器5A残留未融化的霜冷却。
The water drb flowing down is cooled by the lower heat exchanger 5A, which is used as an evaporator, and by frost remaining unmelted on the lower heat exchanger 5A.
图14是表示在结束第二除霜控制时残留于下侧热交换器5A的霜Fr1c的情况的示意图。
Fig. 14 is a schematic view showing a state of frost Fr1c remaining on the lower heat exchanger 5A at the time when the second defrosting control is finished.
第二除霜控制的执行时间比第一除霜控制的执行时间长。
The performance time of the second defrosting control is longer than the performance time of the first defrosting control.
因此，能够通过执行第二除霜控制而融化的霜量比能够通过执行第一除霜控制而融化的霜量多。
Therefore, an amount of frost that can be caused to be melted by performing the second defrosting control is larger than an amount of frost that can be caused to be melted by performing the first defrosting control.
在图14中，图13所示的霜Fr2b完全融化。
In Fig. 14, the frost Fr2b shown in Fig. 13 is caused to be completely melted.
另一方面，图13所示的水drb在下侧热交换器5A的表面结冰，或者因形成于下侧热交换器5A的霜而结冰。
Whereas the water drb shown in Fig. 13 is frozen on the surface of the lower heat exchanger 5A, or is frozen by frost formed on the lower heat exchanger 5A.
特别是，当水drb因形成于下侧热交换器5A的霜而结冰时，下侧热交换器5A的霜变厚，没有与作为热源的下侧热交换器5A接触的霜的量增加。
In particular, when the water drb is frozen by frost formed on the lower heat exchanger 5A, the thickness of frost on the lower heat exchanger 5A increases, so that an amount of frost not in contact with the lower heat exchanger 5A, which is a heat source, increases.
但是，由于在第二除霜控制之前执行第一除霜控制，因此抑制了第三除霜运转开始时的下侧热交换器5A的霜变厚。
However, the first defrosting control is performed before the second defrosting control is performed and hence, frost on the lower heat exchanger 5A is prevented from having a large thickness at the time of starting the third defrosting operation.
图15是表示结束第三除霜控制时的室外热交换器5的示意图。
Fig. 15 is a schematic view showing the outdoor heat exchanger 5 at the time when the third defrosting control is finished.
如上所述，抑制了第三除霜运转开始时的下侧热交换器5A的霜变厚。
As described above, frost on the lower heat exchanger 5A is prevented from having a large thickness at the time of starting the third defrosting operation.
因此，通过执行第三除霜控制，图14所示的霜Fr1c融化。
Therefore, by performing the third defrosting control, the frost Fr1c shown in Fig. 14 melts.
＜实施方式的效果＞
<Advantageous effects of embodiment
以往的制冷循环装置进行上侧热交换器的除霜，之后，进行下侧热交换器的除霜。
An existing refrigeration cycle apparatus performs defrosting of an upper heat exchanger and, subsequently, performs defrosting of a lower heat exchanger.
也就是说，以往的制冷循环装置的室外热交换器的除霜是包括上侧热交换器的除霜以及下侧热交换器的除霜的两个阶段的除霜。
That is, defrosting of the outdoor heat exchanger of the existing refrigeration cycle apparatus is two-stage defrosting including defrosting of the upper heat exchanger and defrosting of the lower heat exchanger.
在以往的制冷循环装置的除霜运转中，当进行上侧热交换器的除霜时，从上侧热交换器流下的水与下侧热交换器的霜接触，从上侧热交换器流下的水因下侧热交换器的霜而结冰。
In the defrosting operation of the existing refrigeration cycle apparatus, when defrosting of the upper heat exchanger is performed, water flowing down from the upper heat exchanger comes into contact with frost on the lower heat exchanger, so that the water flowing down from the upper heat exchanger is frozen by the frost on the lower heat exchanger.
结果，下侧热交换器的除霜开始时的下侧热交换器的霜的厚度会比上侧热交换器的除霜开始时的下侧热交换器的霜的厚度厚。
As a result, the thickness of frost on the lower heat exchanger at the time of starting defrosting of the lower heat exchanger becomes larger than the thickness of frost on the lower heat exchanger at the time of starting defrosting of the upper heat exchanger.
在此，与下侧热交换器接触的霜从下侧热交换器直接受热，因此，与下侧热交换器接触的霜容易融化。
Frost on contact with the lower heat exchanger directly receives heat from the lower heat exchanger, so that the frost on contact with the lower heat exchanger easily melts.
另一方面，没有与下侧热交换器接触的霜例如下侧热交换器的霜的外侧部，接受经由与下侧热交换器接触的霜等传递的热。
Whereas frost not in contact with the lower heat exchanger, for example, the outer portion of the frost on the lower heat exchanger receives heat transferred through the frost or other object in contact with the lower heat exchanger.
因此，下侧热交换器的霜的外侧部不易融化。
Therefore, the outer portion of the frost on the lower heat exchanger does not easily melt.
下侧热交换器的霜的厚度越厚，没有与下侧热交换器接触的霜的量越增加，因此下侧热交换器的霜的厚度越厚，下侧热交换器的除霜效率降低的可能性越高。
As the thickness of frost on the lower heat exchanger increases, an amount of frost not in contact with the lower heat exchanger increases.
但是，制冷循环装置100的控制装置Cnt在执行第二除霜控制之前执行第一除霜控制。
However, the controller Cnt of the refrigeration cycle apparatus 100 performs the first defrosting control before the controller Cnt performs the second defrosting control.
因此，抑制了第三除霜控制开始时的下侧热交换器5A的霜的厚度的增加，结果，抑制了第三除霜控制时的下侧热交换器5A的除霜效率的降低。
Therefore, frost on the lower heat exchanger 5A is prevented from having an increased thickness at the time of starting the third defrosting control and, as a result, it is possible to suppress a reduction in defrosting efficiency of the lower heat exchanger 5A during the third defrosting control.
因此，在第三除霜控制结束时，在下侧热交换器5A中残留未融化的霜的量被抑制。
Accordingly, at the time of finishing the third defrosting control, an amount of frost remaining unmelted on the lower heat exchanger 5A can be reduced.
而且，控制装置Cnt在执行第三除霜控制之后，再次开始制热运转。
The controller Cnt restarts the heating operation after the controller Cnt performs the third defrosting control.
由于在第三除霜控制结束时，在下侧热交换器5A中残留未融化的霜的量被抑制，因此，在执行再次开始的制热运转时，下侧热交换器5A的传热管hpA的制冷剂与通过下侧热交换器5A的空气的热交换的阻碍被抑制。
The amount of frost remaining unmelted on the lower heat exchanger 5A is reduced at the time of finishing the third defrosting control and hence, during the period when the restarted heating operation is performed, it is possible to suppress the inhibition of heat exchange between refrigerant in the heat transfer tube hpA of the lower heat exchanger 5A and air passing through the lower heat exchanger 5A.
因此，在执行在除霜运转之后再次开始的制热运转时下侧热交换器5A的热交换效率的降低被抑制，结果，抑制了制冷循环装置100的制热运转的效率的降低。
Therefore, it is possible to suppress a reduction in efficiency of heat exchange of the lower heat exchanger 5A during the period when the heating operation restarted after the defrosting operation is performed.
列举一例来进行上述效果的补充。
The above-mentioned advantageous effects are additionally described by giving examples.
将第一除霜控制的执行时间与第三除霜控制的执行时间合起来的时间设为X时间，将第二除霜控制的执行时间设为Y时间。
The total time of the performance time of the first defrosting control and the performance time of the third defrosting control is defined as X hours, and the performance time of the second defrosting control is defined as Y hours.
另外，将以往的制冷循环装置的下侧热交换器的除霜时间设为X时间，将以往的制冷循环装置的上侧热交换器的除霜时间设为Y时间。
Further, the defrosting time of the lower heat exchanger of the existing refrigeration cycle apparatus is defined as X hours, and the defrosting time of the upper heat exchanger of the existing refrigeration cycle apparatus is defined as Y hours.
这样，在制冷循环装置100的除霜时间与以往的制冷循环装置的除霜时间相同的情况下，与以往的制冷循环装置的在下侧热交换器中残留未融化的霜的量相比，制冷循环装置100的在下侧热交换器5A中残留未融化的霜的量被抑制。
In this manner, when the defrosting time of the refrigeration cycle apparatus 100 and the defrosting time of the existing refrigeration cycle apparatus are equal to each other, the amount of frost remaining unmelted on the lower heat exchanger 5A of the refrigeration cycle apparatus 100 is reduced compared with the amount of frost remaining unmelted on the lower heat exchanger of the existing refrigeration cycle apparatus.
这是因为，如上所述，制冷循环装置100的控制装置Cnt在执行第二除霜控制之前执行第一除霜控制，因此抑制了第三除霜控制开始时的下侧热交换器5A的霜变厚，结果，抑制了第三除霜控制时的下侧热交换器5A的除霜效率的降低。
As described above, the controller Cnt of the refrigeration cycle apparatus 100 performs the first defrosting control before the controller Cnt performs the second defrosting control.
在实施方式中，制冷循环装置100的第三除霜控制的执行时间是预先设定的。
In the embodiment, the performance time of the third defrosting control of the refrigeration cycle apparatus 100 is predetermined.
但是，如上所述，由于抑制了第三除霜控制开始时的下侧热交换器5A的霜变厚，因此制冷循环装置100的管理者可以不用担心下侧热交换器5A的霜的残留未融化而将第三除霜控制的执行时间设定得超过所需地过长。
However, as described above, frost on the lower heat exchanger 5A is prevented from having a large thickness at the time of starting the third defrosting control and hence, a manager of the refrigeration cycle apparatus 100 is not required to set the performance time of the third defrosting control to a time longer than necessary because of concern for frost remaining unmelted on the lower heat exchanger 5A.
也就是说，制冷循环装置100是容易将除霜运转时间设定得短的结构。
That is, the refrigeration cycle apparatus 100 is configured to easily allow setting of a short time for the defrosting operation.
在此，若能够缩短除霜运转时间，则相应地，从除霜运转恢复到制热运转的定时的延迟也被抑制。
When a time of the defrosting operation can be shortened, it is possible to reduce a delay of timing for returning from the defrosting operation to the heating operation by a corresponding amount.
因此，制冷循环装置100抑制了制热运转的时间相对于包括制热运转的时间及除霜运转的时间在内的总运转时间的比例变小。
Therefore, in the refrigeration cycle apparatus 100, it is possible to suppress a reduction in the ratio of a time of the heating operation to a total operation time including the time of the heating operation and the time of the defrosting operation.
因此，制冷循环装置100具有抑制室内温度的降低的效果。
Accordingly, the refrigeration cycle apparatus 100 has an advantageous effect of suppressing a reduction in temperature of the room.
在制冷循环装置100进行除霜运转的情况下，室内热交换器2作为冷凝器发挥功能。
During the period when the refrigeration cycle apparatus 100 performs the defrosting operation, the indoor heat exchanger 2 is used as a condenser.
具体而言，在控制装置Cnt执行第一除霜控制、第二除霜控制以及第三除霜控制的情况下，室内热交换器2作为冷凝器发挥功能。
Specifically, during the period when the controller Cnt performs the first defrosting control, the second defrosting control, and the third defrosting control, the indoor heat exchanger 2 is used as a condenser.
因此，制冷循环装置100能够一边在室外机20进行室外热交换器5的除霜，一边利用室内机30进行室内的制热。
Therefore, the refrigeration cycle apparatus 100 can perform the heating operation of the room with the indoor unit 30 while performing the defrosting operation of the outdoor heat exchanger 5 with the outdoor unit 20.
在此，为了便于说明，无论是第三除霜控制的执行时间比第一除霜控制的执行时间短的情况，还是第一除霜控制的执行时间比第三除霜控制的执行时间短的情况，都将第一除霜控制的执行时间与第三除霜控制的执行时间合起来的总时间设为恒定。
In this embodiment, for convenience of the description, both in the case where the performance time of the third defrosting control is shorter than the performance time of the first defrosting control and the case where the performance time of the first defrosting control is shorter than the performance time of the third defrosting control, the total time of the performance time of the first defrosting control and the performance time of the third defrosting control is defined to be a fixed time.
在第三除霜控制的执行时间比第一除霜控制的执行时间短的情况下，与第一除霜控制的执行时间长相应地，第一除霜控制中在下侧热交换器5A融化的霜的量增加。
When the performance time of the third defrosting control is shorter than the performance time of the first defrosting control, an amount of frost melting on the lower heat exchanger 5A during the first defrosting control increases by an amount that corresponds to a longer performance time of the first defrosting control.
在此，通过执行第二除霜控制，下侧热交换器5A的结霜量会增加。
At this point of operation, when the second defrosting control is performed, the amount of frost formed on the lower heat exchanger 5A increases.
本发明涉及细胞内传输处理技术领域，特别涉及一种将物质传输到细胞内的系统、总控装置、微控制器及方法。
The invention relates to imaging and manipulation of a biological sample.
在微组装和基因编辑，如DNA及RNA的转染操作中，涉及将外界物质传输到细胞内部的步骤。
Specifically, and not exclusively, the invention relates to imaging and manipulation of a biological sample based on wide-field fluorescence microscopy.
目前的细胞内传输的方案存在以下问题：
BACKGROUND
在传输操作前，需要对细胞进行固定处理，以防止操作时细胞的运动。
Biomedical imaging systems are commonly used to obtain images or video (frames of images) of biological samples or subjects in research, and in the healthcare field for diagnostic and therapeutic purposes.
传统的固定方法，例如利用微针吸附细胞方法等，在较短的时间内，无法固定并排列更多的细胞，使得细胞内传输效率低。
Some exemplary imaging systems include X-rays systems, CT systems, PET systems, ultrasonic imaging systems; magnetic resonance imaging systems, optical imaging systems, etc. These imaging systems rely on different operation principles to obtain images.
传统的细胞内传输方法一般为生物、化学传输方法，例如囊泡，病毒等作为载体等，以及物理方法：电穿孔，微流芯片，磁球等。
Images obtained by these systems are usually processed, analysed, or modified, for improving the quality of the images before the images are presented to the user.
利用这些传统方法进行传输，外部物质只能传输到细胞质内，并间接地扩散到目标细胞器内。
Medical practitioners, biomedical researchers, and the like, rely on images produced by these systems for assessing the state of the biological samples or subjects.
而在DNA转染中，大部分的DNA物质必须在细胞核内实现功能，而细胞质的扩散将降低其的生物活性。
Thus, the quality, time, and cost associated with processing of these images are of significant practical importance.
因此，传统传输方法不适合细胞内物质的传输，传输精度低，使得转染等操作效果差。
SUMMARY OF THE INVENTION
早期的传输操作集中研究了贴壁细胞，而贴壁细胞具有较平整表面形态，其内部的细胞器较容易定位。
It is an object of the invention to address the above needs, to overcome or substantially ameliorate the above disadvantages or, more generally, to provide improved imaging and reliable in vitro manipulation of a biological sample.
而悬浮细胞在细胞培养液中呈现近似圆球的形态，相较于贴壁细胞具有更大的高度。
In accordance with a first aspect of the invention, there is provided a system for manipulating a biological sample, including: an imaging device arranged to image a biological sample; a controller operably connected with the imaging device for processing the images obtained by the imaging device; and a tool manipulation device operably connected with the controller and arranged to be connected with a tool for manipulating the biological sample.
因此，传输部件(内部有待传输外界物质)在不适宜的高度上将很难成功地穿破细胞膜，传输部件在刺破细胞膜过程中细胞会发生倾斜运动。
The controller is arranged to control operation of the tool manipulation device based on the processing of the images.
目前一些研究提出了一些传输部件高度调整算法，例如模板匹配，熵计算法，但是由于细胞器与周围细胞质的图像具有高度地相似性，这些算法无法获取细胞核等细胞器及细胞内微小部件的位置，从而无法精确地将外界物质传输到细胞内部，细胞传输成功率低。
The biological sample may be arranged in a container such as a petri dish, a cover glass, or a microfluidic chip.
传统的诊所操作依赖训练有素的操作员完成细胞内操作，操作员必须人为手动地将微操作工具和目标物体对齐，凭借经验调节微操作工具的位置，实现将外界物质传输到细胞内部。
In one embodiment of the first aspect, the controller is arranged to control operation of the tool manipulation device to effect movement of the tool relative to the biological sample.
疲劳和经验将不可避免地会导致实验的失败，从而降低了细胞传输的效率。
In one embodiment of the first aspect, the tool manipulation device has at least 3 degrees of freedom.
综上，现有将外界物质传输到细胞内部的方案存在：传输精确度低、效率低、成功率低的问题。
The tool manipulation device may have up to 6 degrees of freedom.
针对上述问题，目前尚未提出有效的解决方案。
In one embodiment of the first aspect, the system also includes a tool connected with the tool manipulation device.
本发明实施例提供了一种将外界物质传输到细胞内的系统，用以高效准确地进行细胞内传输，提高传输成功率，该系统包括：显微镜、载物台、物镜、微控制器、微针、微注射器、微流控芯片和总控装置，其中：
The tool may be a manipulation tool, e.g., for intracellular manipulation.
微流控芯片，用于固定待测细胞；
More specifically, the tool may be a surgical tool, e.g., for intracellular surgery.
载物台，用于放置固定有待测细胞的微流控芯片；
For example, the tool may include a micro-pipette, micro-injector, etc.
显微镜的物镜，与总控装置连接，用于在总控装置的控制下，对所述待测细胞进行光学采样，得到采样图片；
In one embodiment of the first aspect, the imaging device is part of an imaging apparatus.
总控装置，用于对所述采样图片进行三维重构处理，得到待测细胞对应的三维重构体；对所述三维重构体进行几何分析，得到最佳传输位置；发送位置调整指令至微控制器；所述位置调整指令中包括：所述最佳传输位置；
In one embodiment of the first aspect, the imaging apparatus includes a support for holding the biological sample, and an objective lens for manipulating light.
微控制器，与总控装置连接，用于根据所述位置调整指令，控制微针和/或载物台移动，使得微针到达被固定待测细胞内的所述最佳传输位置；所述微针内预置有待传输外界物质；
In one embodiment of the first aspect, the imaging apparatus further includes movement means for moving one or both of the support and the objective lens to enable relative movement between the support and the objective lens.
微注射器，与总控装置连接，用于在总控装置的控制下，提供推射压力给微针，将微针内的待传输外界物质传输到细胞内部。
The movement means may be arranged such that the relative movement is in a vertical direction.
本发明实施例还提供了一种将外界物质传输到细胞内的总控装置，用以高效准确地进行细胞内传输，提高传输成功率，该装置包括：
The movement means may further allow relative movement in directions (e.g., on a horizontal plane) other than vertical direction.
采样控制单元，用于控制显微镜的物镜对待测细胞进行光学采样，得到采样图片；所述待测细胞被固定在微流控芯片的沟道中；固定有待测细胞的微流控芯片放置在载物台上；
In one embodiment of the first aspect, the controller is further arranged to control movement of the movement means.
三维重构单元，用于对所述采样图片进行三维重构处理，得到待测细胞的三维重构体；
The controller may be arranged to control movement of the movement means such that the relative movement is in steps.
最佳传输位置确定单元，用于对所述三维重构体进行几何分析，得到最佳传输位置；发送位置调整指令至微控制器；所述位置调整指令中包括：所述最佳传输位置；
The steps are preferably of equal distance.
传输控制单元，用于在微控制器根据位置调整指令控制微针和/或载物台移动，使得微针到达被固定待测细胞内的所述最佳传输位置时，控制微注射器提供推射压力给微针，将微针内预置的待传输外界物质传输到细胞内部。
In one embodiment of the first aspect, the controller is arranged to control movement of the movement means such that the support and the objective lens returns to a default position upon completion of the manipulation of the biological sample by the tool.
本发明实施例还提供了一种将外界物质传输到细胞内的微控制器，用以高效准确地进行细胞内传输，提高传输成功率，该微控制器包括：
In one embodiment of the first aspect, the imaging device is a camera.
接收单元，用于接收位置调整指令；所述位置调整指令中包括最佳传输位置；
The camera may be a CCD camera, a sCMOS camera, or the like, for obtaining images with optical sections of the biological sample.
位置调整控制单元，用于根据所述位置调整指令控制微针和/或载物台移动，使得微针到达被固定待测细胞内的所述最佳传输位置；所述微针内预置有待传输外界物质。
In one embodiment of the first aspect, the imaging apparatus includes a microscope.
本发明实施例还提供了一种将外界物质传输到细胞内的方法，应用于系统，用以高效准确地进行细胞内传输，提高传输成功率，该方法包括：
Preferably, the microscope is a fluorescence microscope.
总控装置控制显微镜的物镜对待测细胞进行光学采样，得到采样图片；所述待测细胞被固定在微流控芯片的沟道中；固定有待测细胞的微流控芯片放置在载物台上；
More preferably, the microscope is a wide-field fluorescence microscope.
总控装置对所述采样图片进行三维重构处理，得到待测细胞的三维重构体；
The wide-field fluorescence microscope may be an inverted wide-field fluorescence microscope or an upright wide-field fluorescence microscope.
总控装置对所述三维重构体进行几何分析，得到最佳传输位置；发送位置调整指令至微控制器；所述位置调整指令中包括：所述最佳传输位置；
The wide-field fluorescence microscope generally includes a light source, dichroic mirror, an excitation filter, an emission filter, and like optical elements, to enable fluorescence imaging.
微控制器根据所述位置调整指令，控制微针和/或载物台移动，使得微针到达被固定待测细胞内的所述最佳传输位置；所述微针内预置有待传输外界物质；
In one embodiment of the first aspect, the controller is arranged to process the images by: deconvoluting the images for removing noises and blurs in the images; segmenting the deconvoluted images; and reconstructing a 3D model of the biological sample based on the segmented deconvoluted images.
总控装置在微针到达被固定待测细胞内的所述最佳传输位置时，控制微注射器提供推射压力给微针，将微针内预置的待传输外界物质传输到细胞内部。
Preferably, the imaging apparatus is a wide-field fluorescence microscope, the imaging device is a camera, and the images are fluorescence images.
本发明实施例还提供了一种将外界物质传输到细胞内的方法，应用于总控装置，用以高效准确地进行细胞内传输，提高传输成功率，该方法包括：
In accordance with a second aspect of the invention, there is provided a method for processing images of a biological sample, including: deconvoluting the images for removing noises and blurs in the images; segmenting the deconvoluted images; and reconstructing a 3D model of the biological sample based on the segmented deconvoluted images.
控制显微镜的物镜对待测细胞进行光学采样，得到采样图片；所述待测细胞被固定在微流控芯片的沟道中；固定有待测细胞的微流控芯片放置在载物台上；
In one embodiment of the second aspect, the images are fluorescence images obtained with a fluorescence microscope.
对所述采样图片进行三维重构处理，得到待测细胞的三维重构体；
In one embodiment of the second aspect, the deconvolution includes: processing the respective images to determine one or more point spread functions for at each optical sampling depth.
对所述三维重构体进行几何分析，得到最佳传输位置；发送位置调整指令至微控制器；所述位置调整指令中包括：所述最佳传输位置；
In one embodiment of the second aspect, the deconvolution further includes: retrieving one or more predetermined one or more point spread functions from the controller based on the processing of the respective images.
在微控制器根据位置调整指令控制微针和/或载物台移动，使得微针到达被固定待测细胞内的所述最佳传输位置时，控制微注射器提供推射压力给微针，将微针内预置的待传输外界物质传输到细胞内部。
In one embodiment of the second aspect, the deconvolution further includes: determining deconvolution estimation of the respective images based on the one or more determined point spread functions.
本发明实施例还提供了一种将外界物质传输到细胞内的方法，应用于微控制器，用以高效准确地进行细胞内传输，提高传输成功率，该方法包括：
In one embodiment of the second aspect, the deconvolution further includes:
接收位置调整指令；所述位置调整指令中包括最佳传输位置；
determining noise regularized estimations of the respective images based on the deconvolution estimation of the respective images.
根据所述位置调整指令控制微针和/或载物台移动，使得微针到达被固定待测细胞内的所述最佳传输位置；所述微针内预置有待传输外界物质。
In one embodiment of the second aspect, the determination of the noise regularized estimations is based on a regularization factor.
本发明实施例还提供了一种计算机设备，包括存储器、处理器及存储在存储器上并可在处理器上运行的计算机程序，所述处理器执行所述计算机程序时实现上述将外界物质传输到细胞内的方法。
In one embodiment of the second aspect, the deconvolution further includes: determining whether the noise regularized estimations approaches convergence to determine completion of the deconvolution.
本发明实施例还提供了一种计算机可读存储介质，所述计算机可读存储介质存储有执行上述将外界物质传输到细胞内的方法的计算机程序。
In one embodiment of the second aspect, the deconvolution further includes: processing the images to turn the images into grayscale images prior to determining the point spread functions.
与传统的细胞内传输方案相比较，本发明实施例提供的技术方案的有益技术效果是：
In one embodiment of the second aspect, the segmentation of the deconvoluted images is based on a localized region-based segmentation method or thresholding.
首先，与传统的细胞内传输方案中固定细胞方法相比较，本发明实施例提供的技术方案中使用微流控芯片固定待测细胞，微流控芯片能够在较短的时间内固定并排列更多的细胞，提高了细胞内传输效率。
In one embodiment of the second aspect, the segmentation of the deconvoluted images further includes: determining whether the segmentation approaches convergence to determine completion of the segmentation.
其次，与传统的传输方法相比较，本发明实施例提供的技术方案中利用微针传递待传输外界物质，采用的微针能够直接穿透多层细胞膜，同时具备精确的传输剂量和时间控制的能力，更适合细胞内物质的传输，提高传输的准确率和效率。
In one embodiment of the second aspect, the reconstruction of the 3D model is based on volume rendering.
另外，与传统的细胞内传输方案中获取细胞器操作信息方法相比较，本发明实施例根据目标细胞的三维重构信息得到最佳的传输位置，极大地避免了微针在刺破细胞膜过程中细胞的倾斜运动，提高了传输成功率。
In accordance with a third aspect of the invention, there is provided a method for operating the system of the first aspect.
本发明涉及车辆的动力系支撑结构。
The present invention relates to a power train support structure for a vehicle.
在车辆中，构成车室前壁的前围板的前方空间通常被用作收纳包含发动机或马达等的动力系的收纳室(所谓的发动机室)。
In a vehicle, generally, a space in front of a dashboard panel constituting a front wall of a passenger compartment is used as an accommodation room (a so-called engine room) for accommodating a power train including an engine or a motor, and the like.
而且，在收纳室中，通常设置有左右一对沿前后方向延伸的前纵梁且通過该左右一对前纵梁来支撑动力系。
It is also general that a pair of left and right front side frames extending in the front-rear direction are disposed in the accommodation room, and the power train is supported by the paired left and right front side frames.
由于动力系是刚体，因此要求在发生前碰撞时抑制该动力系朝向车室内的乘员(驾驶席乘员和助手席乘员)的后退。
Therefore, it is required to suppress the power train from retracting toward a passenger within a passenger compartment (a driver on a driver seat and/or a passenger on a passenger seat) in the event of collision from the front side.
日本专利公开公报特开2007-261529号中公开了如下的结构：支撑动力系的装配支架被安装于前纵梁，在发生车辆的前碰撞时，通过破坏装配支架来使动力系掉落到下方。
Japanese Unexamined Patent Application Publication No. 2007-261529 discloses that, in a structure in which a mounting bracket for supporting a power train is mounted on a front side frame, the power train is allowed to fall downward by breaking the mounting bracket in the event of vehicular collision from the front side.
此处，可考虑将收纳于收纳室的动力系以偏置在左右两侧中的一侧(车宽方向的一侧)的方式来进行设置。
A power train to be accommodated within an accommodation room may be disposed to be offset to one of left and right sides (to one side in the vehicle width direction).
此情况下，驾驶席或助手席便位于动力系的大致正后方。
In this case, the driver seat or the passenger seat is located substantially immediately behind the power train.