Translation requires mRNA, ribosomes, tRNA molecules, and different enzymes to convert genetic information into proteins. During initiation, all the necessary components come together in a precise manner to create the initiation complex, which is essential for beginning the process of protein synthesis. Ribosomes are composed of two subunits: a smaller one and a larger one. In mammals, the small subunit is 40S, and the large subunit is 60S, totaling 80S. In prokaryotes, the small subunit is called 30S, and the large subunit is called 50S, adding up to a total of 70S. The small subunit attaches to the mRNA template, while the large subunit binds tRNA molecules in sequence. The mRNA template carries the genetic code from the DNA to the ribosome, where it's read and translated into a protein sequence. Each tRNA molecule carries a specific amino acid and has a sequence that matches a codon on the mRNA. As the ribosome reads the mRNA, it matches tRNA molecules to the codons and adds the corresponding amino acids to the growing protein chain. Each tRNA also needs to be identified by the appropriate aminoacyl synthetase enzyme. There's a specific aminoacyl tRNA synthetase enzyme for each of the 20 amino acids. Enzymatic factors assist in various steps of the process, such as initiation, elongation, and termination. For example, initiation factors help assemble the ribosome and mRNA to start translation and release factors help stop translation when the protein is complete.