Yes, the days of creation were literal days of 24 hours each. Since in the Bible the same word “day” can sometimes refer to a less defined period of time (for example, “in that day,” “day of salvation,” etc.), some contend that the “days” in the biblical creation account are actually much longer periods of time. However, Genesis 1 clarifies each day as being an “evening” and a “morning.” For this division of a day to be relevant, the day cannot refer to millions of years.