During DNA replication, topoisomerase enzymes would attach to the DNA strands at specific sites to perform their functions. The exact attachment points and actions may vary depending on the type of topoisomerase involved (Type I or Type II). Here are the general locations where topoisomerases would attach during DNA replication: Type I topoisomerases: These enzymes typically bind to the DNA at single-stranded regions or DNA loops. They can help relax supercoiled DNA, remove DNA knots and tangles, and prevent the collapse of replication forks. Type I topoisomerases, such as Topoisomerase 1 in humans, can interact with the DNA ahead of the replication fork to relieve torsional stress caused by the unwinding of the double helix. Type II topoisomerases: These enzymes bind to the double-stranded DNA and are involved in the resolution of intertwined DNA molecules. During DNA replication, Type II topoisomerases, such as Topoisomerase 2 in humans, play a crucial role in managing DNA supercoiling and disentangling DNA strands. They are often found near the replication forks to resolve topological problems arising from DNA replication. It's important to note that the precise binding and action of topoisomerases during DNA replication can be complex and can vary depending on the specific cellular context and the organism being studied. Additionally, different types and subtypes of topoisomerases may have distinct roles and specific binding sites.