Probably not. Interestingly, Ann Gronowski, a pathologist at the University of Washington in St. Louis, and her colleagues examined in a series of studies how some urine-based pregnancy tests used in healthcare and hospital settings can give false negative results five or more weeks into pregnancy. This is because of a flaw in some tests in which they fail to detect a degraded form of hCG, that becomes more prevalent in the body after five or six weeks of pregnancy. However, this likely isn’t a concern with at-home tests. If you think you are pregnant but continuously test negative, you should talk to a healthcare provider.