No, the media is not interchangeable. To generate the most representative cultures of the large and small airways, we recommend differentiating HBECs using PneumaCult-ALI Medium and HSAECs using PneumaCult- ALI-S Medium, respectively. Compare the morphology and marker expression of HSAECs and HBECs cultured in PneumaCult-ALI-S Medium or PneumaCult-ALI Medium (Figure 3 and 4)