No it will not. All Good Life products are designed to be safe and humane, and they are only designed to stop excess or nuisance barking. The high-pitched training sounds are not painful or harmful, and if a dog really needs to bark, he can. They will not stop a dog from barking out of instinct (at an intruder for example). Although not harmful, the sounds emitted by our products may be too intense for puppies under one year of age as they are still developing their hearing. Please consult your veterinarian before using this product with puppies.