Cells that are thawed directly into blood-free media typically recover well from cryo-preservation. Addition of the individual components, such as Cellastim S, can facilitate different processes in cell culture such as single cell cloning. For example, addition of Cellastim S, Optiferrin, and ITSE to commercial CHO media can reduce timelines in CHO cell line selection from single cell cultures to having adequate number of cells to analyze growth and expression potential of clonal populations.