To compensate for peripheral blood dilution due to with increasing volumes of aspiration from a single location within the marrow space, clinicians have used centrifugation protocols to volume reduce 50 to 60 mL of aspirate to 7 to 10 mL by removing excess plasma and red blood cells from the peripheral blood. The final treating composition typically has a concentration above baseline of nucleated cells from the peripheral blood and the marrow.
Muschler GF, et al. Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume. J Bone Joint Surg Am. 1997 Nov;79(11):1699-709.
Hegde V, et al. A prospective comparison of 3 approved systems for autologous bone marrow concentration demonstrated nonequivalency in progenitor cell number and concentration. J Orthop Trauma. 2014 Oct;28(10):591-8.