The centrifugation step is optional. If the lysate will be stored long-term, then centrifugation and transfer of the supernatant to a fresh tube before dilution in a buffered solution is recommended. Centrifugation of the reaction product for 1 minute at high speed is sufficient to pellet debris, and the supernatant is easily recovered. In some cases, the debris does not pellet but rather remains suspended throughout the extract. In such cases, it is recommended that the DNA-containing liquid be carefully transferred to a fresh tube for downstream use and storage.