1.) Waterproof or water-resistant materials such as oils or powders must not be sterilized. 2.) Overload the autoclave with the vessel and equipment. Sterilize your equipment as low as possible, if possible. 3.) Only autoclavable bags should be used for autoclave package waste. 4.) Use autoclavable bags to sterilize your equipment. Never use aluminum foil in this. 5.) Do not fill the autoclave chamber to the lid.