Both walking and running activate the glutes. While walking works mainly your quads and calves, you can still tone your glutes by squeezing your bum with each step or incorporating hill walks or stair climbs to further engage the muscles. As for running, different paces will recruit either fast or slow-twitch muscle fibers which will activate your glutes in their own way. For example, endurance runs build up your type I (slow-twitch) muscle fibers, that use oxygen with aerobic metabolism to carry you for longer periods before fatigue sets it. Whereas sprints require type II (fast-twitch) muscle fibers that work with the anaerobic metabolism to fire quicker and produce more speed and power, although for shorter periods.