Yes. However, it is advisable to lay off someone who is not pregnant if at all possible. It is a presumption under the law that a negative change in the employment status of a pregnant woman is due to her pregnancy, and this presumption is difficult to disprove. If the decision to lay off employees is genuine and necessary for your business, and some are pregnant, there is some justification for your actions. This does not mean, however, that your company will not wind up paying a significant amount of money justifying its decision. It is a human rights violation and a breach of an employment standard in Ontario to lay off a pregnant woman just because she is pregnant. A bona fide reason must be presented for this action.