No. Unlike some other threshold temperature products, Timestrip PLUS does not require pre-conditioning. You need to activate above the product's threshold temperature, as you cannot squeeze the dye out of the blister if it is in solid form. Immediately after activation, stop the progress of the dye by placing the indicator below its STOP temperature. You can adhere the Timestrip immediately to a product or carrying card which is below the STOP temperature. Alternatively place the activated indicator in an environment much colder than the STOP temperature for a short period of time.