Methylene blue also stimulates glucose metabolism by increasing glucose uptake and utilization in the cells. Glucose is the main source of energy for most cells, especially the brain cells. Methylene blue can activate the enzyme hexokinase, which converts glucose to glucose-6-phosphate, the first step of glycolysis. Glycolysis is a metabolic pathway that breaks down glucose into pyruvate, which can then enter the mitochondria for further oxidation. By stimulating glucose metabolism, methylene blue can provide more fuel for the mitochondria and enhance their function.