Yes, you can definitely bore sight at 25 yards. Bore sighting is the practice of aligning the sights or crosshairs of a firearm with the barrel, so that the firearm is accurately aimed when fired. The purpose of bore sighting is to eliminate or reduce the need to aim the firearm after sighting in, which can be done at a closer distance. For most people who simply shoot at the range or use their weapon for self-defense, 25 yards is great!