Yes, your spouse's income can be used for your VA loan application, as long as they are contractually obligated on the loan.
In cases where the spouse is not obligated on the VA loan, the laws of the state may determine whether or not the spouse's income may be counted. These "community property laws" are based on the shared financial obligations the married couple has.
If the borrower resides in a community property state, spouse income may be counted. If the borrower does not reside in a community property state, chances are that the only way spouse income might be counted is by having the spouse obligated on the new loan.