Muscular tissues of Lithuanian canids, mustelids, raccoons, felines, and birds of prey are planned to be examined for these parasites. The mammals and birds of prey will be collected in collaboration with the Tado Ivanauskas Museum of Zoology, the national authority responsible for monitoring dead and injured wild animals. Artificial digestion and microscopic methods will be used to detect parasites in muscles of hosts. Histopathological tests will be used to determine pathogenicity. Different PCR variants, sequencing and bioinformatic analysis of the DNA sequences obtained will be used for species identification. Statistical analysis of the data will be carried out using the SPSS package. The objectives of the study will be: 1) to identify the composition of parasites in predator muscle; 2) to determine the transmission potential of parasitic zoonoses and potential hosts; 3) to assess the level of pathogenicity of Sarcocystis parasites; 4) to evaluate the intra- and inter-species variability of the parasites studied by means of DNA sequence analysis.