Yes, but only if you have 20 lanes (8 PCIe lanes for the top x16 PCIe slot; however, the B550 Aorus Master has just 8 channels for the top PCIe slot, and 3 x4 M. 2 NVM 4.0 PCIe 4.0 slots, which is an outlier.). The B550 motherboard's other PCIe lanes are all PCIe 3.0.