No, only standard ELISA equipment is required.  An automated plate reader with a generic software that reads at A450 and A650 wavelengths is required. Data analysis can easily be performed in Excel using the template provided on the Downloads page. Plates can be washed manually (using bath or pipette techniques) or with an automated plate washer, for which the program should be carefully optimised. The use of electronic or manual multi-channel pipettes is highly recommended. For more information, please see the Materials and Equipment document on the Downloads page.