Assembly: The PN40024.v4 assembly is based on the Sanger scaffolds of the 12X.2 version that were corrected, extended and completed using 30X PacBio data. The heterozygous regions are better identified (Manuscript in preparation).   Access: ENA submission: https://www.ebi.ac.uk/ena/browser/view/PRJEB45423   __________________________________________________________ NEW ANNOTATIONS   4.3 Annotation version that includes manually curated genes from the Annot´training School, held in Zaragoza June 2022. PN40024.v4.3 annotations (gff) => original_PN40024_pseudomolecules.v4.3.BETA.gff3     4.2 Annotation version that includes manually curated genes from the DAP&CAT Working group meeting, held in Valencia July 2021. PN40024.v4.2 annotations (gff) => PN40024.v4.2.REF.gff3 __________________________________________________________ The following download gives access to (click here)    Pseudomolecules of the 19 chromosomes + chrUn => PN40024.v4.REF.fasta Allelic version of the heterozygous regions => PN40024.v4.ALT.heterozyguous_regions.fasta PN40024.v4.1 annotations (gff and fasta) => REF_annotations/ PN40024.v4.1 annotations (gff and fasta) of the allelic version of the heterozygous regions => ALT_annotations/ Functional annotations generated with Blast2GO => Blast2GO_results_PN40024_september_2021/                 Stats and infos => Blast2GO_results_PN40024_september_2021/PN40024.v4.1.REF.b2g.pdf                 Functional annotation file (full info) => Blast2GO_results_PN40024_september_2021/PN40024.v4.1.REF.b2g.tsv                 Functional annotation file (condensed info) => Blast2GO_results_PN40024_september_2021/PN40024.v4.1.REF.b2g.annot   Annotation: The PN40024.v4 annotation is based on a mixed approach, derived from de novo annotation and reciprocal matching with the previous structural annotation version. In an effort to preserve VCOST.v3 gene IDs where possible (e.g. in cases where chromosome numbers are unchanged) a reciprocal best-hits strategy between VCOST.v3 and PN40024.v4.1 annotations was carried out. This allowed the transfer of 67.27% of VCOST.v3 gene IDs. In general, the new de novo annotation performed better than the previous annotation in terms of BUSCO stats. INTEGRAPE will soon offer a Training School for gene model manual curation using several tools such as WebApollo, JBrowser and the Gene Tree tool made available by the Gramene Database (Dr. Doreen Ware).   NEW => Blast sequence server: http://138.102.159.70:4567/ NEW => Apollo for manual curation: http://138.102.159.70:8080/apollo/annotator/index (request an account by email to Amandine Velt and/or Camille Rustenholz) NEW => Jbrowse (under construction): http://138.102.159.70/jbrowse/   Provider Camille Rustenholz / Amandine Velt (Assembly-Annotation) Bianca Frommer (Annotation) Primary contact | camille.rustenholz (at) inrae.fr | amandine.velt (at) inrae.fr