Because the titer of each batch of lentiviral particles varies, the volume required for transduction will also vary. The titer can be found on the Certificate of Analysis (CoA) provided with each lentiviral vector batch. Use the formula below to calculate the volume of lentiviral vector for transduction. Please refer to the product user manual for additional information.
Viral vectors volume required (µl) = Number of cells counted on the day of the transduction / Viral vectors Titer (TU/ml) x M.O.I x 1000