We do not currently have a protocol for predicting successful differentiation. This in part due to our observation that the profile of successful differentiation is dependent on the downstream differentiated cell of interest. For example, we find that hepatocytes and cardiomyocytes have unique secretory profiles when differentiated from iPSCs. We suggest that each lab independently determine the secretory profile for their differentiated cell type of interest. This can be done qualitatively using the Proteome Profiler Arrays, followed by the more quantitative Luminex Assays.