For our eDNA filter kits we use a salt and detergent-based lysis solution. This solution is non-hazardous, stable at room temperature, and doesn’t have the logistical difficulties associated with ethanol, which is another type of preservative which can be used. Ethanol is required to be used currently for Great Crested Newt tests in the UK. We have a range of preservatives that we use for other types of sample e.g. soil and insects, which we chose depending on the project, location, speed from sampling to processing etc.