MSD Electrochemiluminescence or electrogenerated chemiluminescence is the emission of light by electrochemical assay reactions. All Meso Scale Discovery electrochemiluminescence assays (MSD ECL assays) utilize 96 well plates that contain single spot or multiple spots on the bottom of each well. These spots have electrodes that bind to various capture molecules such as antibodies, antigens, peptides, and lysates. After the capture molecules are attached to the plate, the samples and detection antibody conjugated to an MSD electrochemiluminescence tag (SULFO-TAG) are added over a series of incubation and wash steps. Once the MSD mesoscale assay is complete, the user adds a read buffer, and a voltage is applied to the plate electrodes to activate the SULFO-TAG for detection. Now, the emitted light intensity gets measured by the Meso Scale Discovery (MSD) platform to quantify various analyte concentrations in your study samples. Using this relatively new technology, MSD ELISA exhibits high sensitivity and a more comprehensive dynamic range than traditional methods.