Vo filme Tom a Jerry: Sherlock Holmes hrajú Michael York (Sherlock Holmes (voice)), Malcolm McDowell (Professor Moriarity (voice)), John Rhys-Davies (Dr. Watson (voice)), Billy West (Tom (voice)), Grey DeLisle (Red (voice)), Phil LaMarr (Spike / Policeman (voice)), Kath Soucie (Tuffy (voice)), Jeff Bergman (Butch / Droopy (voice)), Greg Ellis (Tin / Sergeant (voice)), Jess Harnell (Pan / Brett Jeremy (voice)), Richard McGonagle (Ali / First Policeman (voice)).