After you’ve sterilized a bottle, you can expect that bottle to remain sterile for up to 24 hours. Past this point, the bottle is no longer considered sterile.  If a baby bottle you wish to use hasn’t been sterilized in the past 24 hours, you can simply quickly sterilize it again before using it. Some sterilizers even dry your bottles after sterilizing, making it a quick, easy, and convenient option to use before feeding your baby.