Yes. But the procedure differs between Windows and Mac. For MAC users, all you have to do is plug in your two interfaces, access “audio devices,” click the + button on the bottom left corner, and click on “create aggregate device,” where you add your device and your audio interfaces’ inputs and outputs. It's a little trickier for Windows users. You will need to download "ASIO4ALL" from asio4all.com, install it, and then connect both of your audio interfaces. Once you've completed these first two steps: 1- Open Ableton Live, click on "options" then "preferences." 2- Click the Audio tab, set your Driver Type to ASIO, and select ASIO4ALL as your audio device. 3- Click on "hardware setup," then the "Advanced view"(or the screwdriver), and click on all the inputs and outputs that you'd like to activate. 4- Click on "input config" and activate all of them, then repeat the same process in "output config." Your audio interfaces should now be completely functional with Ableton Live.