To make sterile water, bring water to a rolling boil for two minutes and allow it to cool for 20 to 30 minutes. You can store sterile water in sterile glassware with a clean lid. Sterile water should be used within 24 hours. Many parents find it easiest to boil water in the morning to last for the whole day. Most bottled water is not sterile, unless the label specifically states “sterile” on the label. Please check with your healthcare provider if you have questions about the need to use sterile water to prepare Neocate Infant DHA/ARA.