Ve filmu Anna Nicole hrají Agnes Bruckner (Anna Nicole Smith), Martin Landau (J. Howard Marshall), Virginia Madsen (Virgie Arthur), Adam Goldberg (Howard K. Stern), Cary Elwes (E. Pierce Marshall), Al Vicente (Cole Harris), Graham Patrick Martin (Older Danny), Vickie Eng (Plastic Surgeon), Carla Fisher (Black Dancer), Alexa Blair Robertson (14 Year Old Vicky Lynn), Jay Huguley (John Lawfton), Alex Van (Ben Walker), Luke Donaldson (Young Danny), Caleb Barwick (Middle Danny), William Day (Vicky Lynn's Father), James Allen McCune (Tommy Smith), David Dwyer (Burke Jones), Jena Sims (Third Dancer), Emmanuel Barbe (Georges Marciano), Danielle Sebille (Paul Marciano), Cristina Franco (Sylvan), Joe Knezevich (Maurice), Billy Slaughter (Producer #1), Greg Corbett (Producer #2), Julio Cotto (DJ), John Tillapaugh (CEO), Brittany Susko (Gal Pal), Danny Boushebel (Sali Elaha), Andrew Sheer (Paparazzi #1), Jesse Malinowski (Party Boy #1), Anna Colwell (Reservations Girl), Sasha Rionda (Reporter #1), Greg Clarkson (Reporter #2), David Kronawitter (Reporter #3), Dayo Abanikanda (Paparazzi #2), Phoebe Holston (Counselor), Tara Jones (Reporter #1 - Airport), Ray D'Alessio (Reporter #2 - Airport), Beth Keener (Reporter #4 - Airport), Thomas Mikusz (Philip), Donny Boaz (Larry Birkhead), Callie McClincy (Hanna James), Mike Senior (Bahamian Doctor), Neal Hazard (Priest), Bonnie Johnson (Secretary), Chris Cox (David Letterman sound-alike), David Alessi (Club Patron (uncredited)), Rachele Bailey (Winners Circle Guest (uncredited)), Loren L. Baker (LAX Traveler (uncredited)), Starlin Britt (Paulo (uncredited)), Tony Caudill (Race Official (uncredited)), Gavyn Tyler Dye (Toddler Danny (uncredited)), Jeff Glover (Paparazzi / Hollywood Party Guest (uncredited)), Drew Grey ((Uncredited)), Rory Healy (Winner's Circle Interwiever / Airport Paparazzi /Reality Show /Boom Operator (uncredited)), Angela Kerecz (Wedding Guest, Party Guest (uncredited)), Bobby Kerecz (Wedding Guest (uncredited)), King (Photographer Assistant (uncredited)), Larry King (Himself (uncredited / archivefootage)), Matt Lauer (Himself (uncredited / Archivefootage)), Cecil Love (Jewelry Store Salesman (uncredited)), Carla McCullough (Maid (uncredited)), Trey McGriff (High Society, Wealthy Friend /Party Guest of the Groom (uncredited)), Matthew Prokopek (Pizza Server (uncredited)), Jim Recznik (Precision Driver (uncredited)), Joel Rogers (Upscale Party Friend (uncredited)), Rob Ruprecht (Hotel Frat Boy (uncredited)), Josh Turner (Anna Nicole's Wedding Guest (uncredited)), Jackson Walters (Baby Danny (uncredited)), Pete Williams (Himself (uncredited)), Julia Walters (7 Year Old Vicky Lynn).