Depending on the size of the bioreactor different separation principles are used for perfusion processes. Small scale perfusion processes can be performed in AMBR15 as a so-called SAM perfusion, a sedimentation-based perfusion method, or conducted in SPINTube as a mock perfusion process (centrifugation based). For larger scale bioreactors Xcell ATF or Centritech separation devices are available and can be applied depending on the shear stress sensitivity of a production clone. (Ref.: Kreye, Steffen & Stahn, Rainer & Nawrath, Karina & Goralczyk, Vicky & Zoro, Barney & Goletz, Steffen. (2019). A novel scale‐down mimic of perfusion cell culture using sedimentation in an automated microbioreactor (SAM). Biotechnology Progress. 35. e2832. 10.1002/btpr.2832.)