The highest payout in roulette is 35 to 1. This payout is awarded if you correctly predict the exact number that the roulette ball lands on, which is known as a straight-up or single number bet. In addition to straight-up bets, there are other types of bets that offer different payouts, such as split bets, street bets, corner bets, and more. However, the highest payout is always offered for the most challenging and risky bet, which is the straight-up bet.