Hand-washing a Nylabone with a brush and non-toxic detergents followed by rinsing with warm water is the best and safest way to sanitize it.  Nylabones should not be boiled in water or put in microwave ovens or dishwashers in order to sanitize them. By using these methods, the fibers of the Nylabone can be broken down and weakened, making it more likely that your dog will chew off pieces.