Yes, you can. When ripping DVDs, whether you can preserve subtitles and multiple audio tracks depends on the software you use and the settings you choose. WinX DVD Ripper allows you to select the audio tracks and subtitles you want to include in the ripped file. When using ripping software, ensure you select the option to include subtitles and multiple audio tracks during the ripping process. Plex will recognize and allow you to choose these options when playing the media.