Autoclaving is popularly also called steam sterilization and pressurized steam is used to kill any infectious agents and denature proteins. It is a very dependable method and can kill any fungi, spores viruses, and bacteria. The autoclave delivers pressure and allows the steam to flow around the items in the chamber. The length of time and temperature necessary for sterilization depends on the items to be sterilized and whether they are wrapped or left directly exposed to the steam.