Vo filme Temný rytier hrajú Christian Bale (Bruce Wayne / Batman), Heath Ledger (Joker), Michael Caine (Alfred Pennyworth), Gary Oldman (James Gordon), Aaron Eckhart (Harvey Dent / Two-Face), Maggie Gyllenhaal (Rachel Dawes), Morgan Freeman (Lucius Fox), Nestor Carbonell (Anthony Garcia), Monique Gabriela Curnen (Anna Ramirez), Ron Dean (Michael Wuertz), Chin Han (Lau), Eric Roberts (Sal Maroni), Ritchie Coster (Chechen), Anthony Michael Hall (Mike Engel), Keith Szarabajka (Gerard Stephens), Joshua Harto (Coleman Reese), Melinda McGraw (Barbara Gordon), Nathan Gamble (James Gordon Jr.), Michael Jai White (Gambol), Colin McFarlane (Gillian Loeb), Nydia Rodriguez Terracina (Janet Surrillo), Tommy Lister Jr. (Tattooed Prisoner), William Fichtner (Bank Manager), Cillian Murphy (Jonathan Crane / Scarecrow), Patrick Leahy (Gentleman at Party), Matt Skiba (Man Trying to Reach Coleman Reese (uncredited)), David Dastmalchian (Thomas Schiff), Michael Vieau (Rossi), Michael Stoyanov (Dopey), William Smillie (Happy), Danny Goldring (Grumpy), Matthew O'Neill (Chuckles), Olumiji Olawumi (Drug Dealer), Greg Beam (Drug Buyer), Erik Hellman (Junkie), Beatrice Rosen (Natascha), Vincenzo Nicoli (Crime Boss), 陳冠希 (LSI VP), Andy Luther (Brian), James Farruggio (Man No. 1), Tom McElroy (Man No. 2), Will Zahrn (Assistant DA), James Fierro (Thug at Party), Sam Derence (Male Guest), Jennifer Knox (Female Guest), Patrick Clear (Judge Freel), Sarah Jayne Dunn (Maroni's Mistress), Charles Venn (Gambol's Bodyguard), Winston Ellis (Gambol's Bodyguard), Sophia Hinshelwood (Reporter), Keith Kupferer (Heckler), Joseph Luis Caballero (Cop Heckler), Richard Dillane (Acting Commissioner), Daryl Satcher (Officer at Intersection), Chris Petschler (Convoy Leader), Aidan Feore (Fat Thug), Philip Bulcock (Murphy), Paul Birchard (Cop with Fat Thug), Walter Lewis (Medic), Vincent Riotta (Cop at 250 52nd St.), Nancy Crane (Nurse), K. Todd Freeman (Polk), Matt Shallenberger (Berg), Michael Andrew Gorman (Cop at Hospital), Lanny Lutz (Bartender), Peter DeFaria (Civilian), Matt Rippy (First Mate), Andrew Bicknell (Prison Ferry Pilot), Ariyon Bakare (Guard Commander), Doug Ballard (Businessman), Helene Maksoud (Mother), Tommy Campbell (Passenger), Craig Heaney (Passenger), Lorna Gayle (Passenger), Lisa McAllister (Passenger), Peter Brooke (Passenger), Joshua Rollins (SWAT Sniper), Dale Rivera (SWAT Leader), Matthew Leitch (Prisoner on Ferry), Thomas Gaitsch (Reporter #3), William Armstrong (Evans), Adam Kalesperis (Honor Guard Man), Tristan Tait (Uniform Cop), Bronson Webb (Bounty Hunter), David Ajala (Bounty Hunter), Gertrude Kyles (Fox's Secretary), Jonathan Ryland (Passenger Ferry Pilot), James Scales (Guardsman), Nigel Carrington (Warden), Ian Pirie (Corrections Officer), Lateef Lovejoy (Prisoner), Grahame Edwards (Prisoner), Roger Monk (Prisoner), Ronan Summers (Prisoner), Wai Wong (Hong Kong Detective), Michael Corey Foster (Honor Guard Leader), Hannah Gunn (Gordon's Daughter), Jon Lee Brody (Waiter (uncredited)), Debbi Burns (Bank Patron (uncredited)), Maritza Cabrera (Party Guest (uncredited)), Shirin Caiola (Party Guest with Glass (uncredited)), Laura Chernicky (Party Guest (uncredited)), Henry Milton Chu (Lau Henchman (uncredited)), Kelli Clevenger (Paramedic (uncredited)), Richard Divizio (Chechen Gangster (uncredited)), Tony Domino (Press Conference Heckler (uncredited)), David Fultz (Pedestrian (uncredited)), Natalie Hallam (Ferry Passenger (uncredited)), Jordon Hodges (Police Officer (uncredited)), Erron Jay (Prisoner (uncredited)), Nicky Katt (Shotgun SWAT (uncredited)), Thomas Kosik (Parade Police Officer (uncredited)), Don Kress (Maroni's Henchman (uncredited)), Tim Krueger (Assistant D.A. (uncredited)), Dan Latham (Police Sgt. Spellman / Gotham Bomb Squad (uncredited)), Tom McComas (Helicopter SWAT Sniper (uncredited)), James Mellor (Ferry Passenger (uncredited)), Joseph Oliveira (Officer (uncredited)), Buster Reeves (Joker's Thug #2 (uncredited)), Peter Rnic (Prisoner (uncredited)), Amit Shah (Party Guest (uncredited)), Michelle Shields (Angry Hospital Relative (uncredited)), Sofiya Smirnova (Evacuee (uncredited)), Bruce Spielbauer (High-Ranking Police Official (uncredited)), Robert Patrick Stern (Extra (uncredited)), Robert Stone (Dept. of Corrections Resident (uncredited)), Richard Strobel (Detective (uncredited)), Tom Townsend (Police Officer (uncredited)), John Turk (Chechen's Bodyguard (uncredited)), John Warman (Detective (uncredited)), Chris Wilson (Major Crime Unit Detective (uncredited)), Kevin Zaideman (Party Staff (uncredited)), Rob Clark (Party Guest (uncredited)), Brandon Lambdin (Armoured Car SWAT), Craig Braginsky (Police Officer (uncredited)), Mike Whyte (Police Officer (uncredited)), Tina Simmons (Detective (uncredited)), Rick Avery (Masked Thug (uncredited)).