If there is only very minimal starting material and therefore a correspondingly low concentration of cDNA is available.
The optimised reflection properties of the wells of white Multiply PCR plates effectively prevent 'crosstalk' between the wells and increase the fluorescence level of the qPCR reaction by a factor of 10. In this way, reliable Ct values can still be generated through the common qPCR cycles even when there is only a small volume of starting material.
If you are aiming to achieve the lowest possible variability.
The optimised reflection properties of the white Multiply PCR plates ensure an even and stable fluorescence level of the qPCR reaction. This leads to a significantly improved reproducibility of the measurement results and increased accuracy. Tip: In order to further improve the variability, we recommend processing at least the template DNA and better still all of the reagents used, with our low retention pipette tips.
If you are aiming to save reagents.
The optimised reflection properties of the wells of white Multiply PCR plates increase the fluorescence level of the qPCR reaction by a factor of 10. This means that the volume of reagents used can be reduced significantly, effectively reducing costs.