The process of regulation of gene expression in eukaryotes is complex which creates more opportunities for the regulation of gene expression in eukaryotes. The mechanism of gene regulation has two important levels i.e. regulation of gene at the level of transcription and post transcription. At the level of transcription, transcription factors bind to regulatory sequences(enhancer, silencer) to control gene expression. Post transcription mechanisms involve steps like mRNA processing(RNA splicing), mRNA stability, prevention of translation of mRNA to avoid the production of the gene product. Chromatin remodelling also plays role in the regulation of gene expression by altering the structure of chromatin. Some epigenetic changes are also involved like DNA methylation, histone modification in the process of gene expression control.