The biggest win available on Da Vinci Diamonds can reach up to 25,000 times the player's initial stake. It can vary depending on various factors, including the player's bet size, the specific combination of symbols that land on the reels, and the game's current settings. However, in general, the game offers the potential for substantial wins, with the highest-paying symbol being the Da Vinci Diamonds logo. Hitting multiple of these symbols on a payline during a single spin or bonus round can result in significant payouts, potentially reaching hundreds or even thousands of times the player's initial bet. So, the biggest win available in the game can be quite substantial and is determined by a combination of luck and strategic gameplay.