Yes and no. If you do not have access to Disney's Extended Evening Hours or a ticket to a special event such as Mickey's Not-So-Scary Halloween Party or Mickey's Very Merry Christmas Party, you can only ride TRON Lightcycle/Run (Magic Kingdom) or Guardians of the Galaxy: Cosmic Rewind (EPCOT) with a Virtual Queue once per day. A boarding group can be obtained either at 7 AM or 1 PM. If you have access to Extended Evening Hours or a special ticketed event, and you obtained a boarding group at 7 AM or 1 PM, you can try to another Virtual Queue boarding group at 6 PM.