When imaging, the smaller the pixel, the better the resolution. With smaller pixels, you need to have better sensitivity and you also need to be careful of noise, so a Peltier-cooled scientific CMOS camera is the preferred option. We use a 2k × 2k scientific camera with a quantum efficiency of more than 80%. However, the main limitation is the sample. If you have a brighter sample, your camera’s exposure time decides the frame rate, and you won’t have to acquire multiple images to construct one super resolution image. So, if for every exposure you get one super-resolved image, you reach up to 100 frames per second. In addition, the system gives you the option to have two cameras, enabling you to image two colors simultaneously. However, the system also comes with a built-in fast emission filter that enables you to do fast sequential multicolor imaging, depending on the number of lasers and emission filters.