Absolutely! Every summer, my sister, her kids, and I visit a local blueberry field and pick pound after pound of fresh blueberries. I wash, dry, and then freeze them to enjoy all year long. These are the blueberries I used for this extract, and they turned out great! As the blueberries thaw, they'll naturally start to release juices and color. If you use fresh berries, you may want to mash them before you add the vodka to speed up this process.