Cruises depart from Kewalo Basin Harbor - Slip F21, located at 1009 Ala Moana Blvd., Honolulu, HI 96814. Kewalo Basin Harbor is about a 15 to 20-minute drive from most resorts in Waikiki.
Look for the big red sign that reads "Hawaii Glass Bottom Boats." You can find it at the front of the harbor along Ala Moana Boulevard, close to the entrance near the cross street at Ward Ave.