Yes! The Abwiz Bio team has deep rooted experience developing and optimizing rabbit mAbs. Our expertise paired with our novel humanization, Rabwiz and STEM methods make rabbit antibody development and humanization routine and optimized.
Our proprietary phagemid vector is engineered to optimally express native rabbit Fab fragments that have an extra di-sulfide bridge between the 2 cysteines in the framework 3 and CK1 of light chains (dramatically improving the diversity of obtained clones).
Through systematic sequencing and cloning of candidate clones into a mammalian expression vector and transient transfection in HEK293 cells, we can quickly humanize (within 2 months) rabbit antibodies and further improve affinity and specificity with our unique STEM method.