Specific diagnosis and treatment of kala-azar are problematic for many reasons. Treatment is lengthy and costly. The diagnosis is invasive and obtained by organ needle aspiration for microscopic detection. Bone marrow, spleen, and lymph node tissues are sampled in case of suspected infection. The diagnostic sensitivity of spleen aspiration is higher- 95 % to 98 %. However, the procedure carries a lot of risk of bleeding. Bone marrow testing accuracy is considered lowest- 53% - 95%. Organ aspirations and examinations require technical skills that are not available in rural areas. Correct diagnosis methods are not undertaken outside of research laboratories.