Biofilm is a structured community of microorganisms that adhere to surfaces, encompassing bacteria, fungi, and other microbes. This amalgamation forms a protective matrix composed of substances like proteins and polysaccharides. Biofilms can manifest in various settings, including medical equipment, pipes, and natural environments like rivers. Due to their resilience and ability to withstand antibiotics and disinfectants, biofilms pose challenges in fields such as healthcare and industry. Understanding biofilms is crucial for developing strategies to manage their impact and improve hygiene and health standards.