Pros of membrane permeability assays:
Membrane permeability assays are generally quick and straightforward to perform.
Results can be obtained in a relatively short period of time.
Exclusion dye propidium iodide has a very bright color making it easy to distinguish live cells from dead cells.
Membrane permeability assays are versatile in that they can be used with a wide range of cell types. They can also be adapted to many different experimental conditions.
The quantitative measure of cell viability and cytotoxicity can be easily obtained by quantifying the extent of membrane permeability.
Many membrane permeability assays utilize commonly available reagents, making them cost-effective options for determining viability and cytotoxicity.