All Mesoscale Discovery Immunoassays are based on ECL technology and provide a broad dynamic range and accurate results from a minimal sample volume. The Meso Scale Discovery multiplex assays are offered in five typical assay formats depending on your needs and applications:
V-PLEX: These are the highest quality assay panels from MSD. They are validated according to fit-for-purpose principles and contain controls with acceptance criteria to monitor plate-to-plate variability. These assays are available as a single validated assay or as part of a multiplex panel. All V-PLEX panels, including MSD cytokine assays, have been validated for serum, plasma, urine, and cell culture supernatants for multiple species.
U-PLEX: This assay format is the most flexible and allows you to customize your own Meso Scale Discovery multiplex panels. You can create panels of up to ten (10) compatible analytes from a comprehensive MSD biomarker assays menu or with custom antibody pairs. Meso Scale Discovery U-PLEX immunoassays are available in multiple species and demonstrated to work on serum, plasma, and cell culture supernatants.
R-PLEX: These kits provide matched antibody pairs for building your own customized MSD assay. Each R-PLEX kit contains a biotinylated capture antibody, a detection antibody conjugated to a SULFO-TAG, reference calibrators, and recommended diluents. Sometimes, it’s possible to perform these highly sensitive R-PLEX assays in an open spot on a U-PLEX panel as per your sample dilution requirements.
S-PLEX: This small group of MSD assays specializes in ultra-sensitive singleplex assays that can reduce the lower detection limit by 10-1000 fold. These assays involve using reagents that help enhance and generate the assay signal on the Meso Scale Discovery (MSD) platform.
T-PLEX: These MSD assays are ready to use single and disease-specific multiplex assays that include some of the most widely studied analytes. These Meso Scale Discovery multiplex panels are available for various species and are compatible with a broad range of sample types to study both secreted and intracellular biomarkers.