BOTOX generally starts working in about 5 to 7 days, with most of it settled in at 10-14 days. Dysport generally starts working at about 3-5 days, with most of it settled in at 10-14 days. We generally recommend that patients give it 2 weeks before coming in for any "touch up" procedures, to allow for ample time for the BOTOX/Dysport to take effect.