Anti-fading is a technique used in fluorescence microscopy to reduce the loss of fluorescent signals over time. It allows researchers to study a wide range of samples with a high level of precision and accuracy. Anti-fading is commonly used to study biological samples, particularly in live cell imaging. Types of samples that can be studied using anti-fading include tissue samples, fluorescently labeled cells, and microorganisms. Using antifading increases the photostability of fluorophores in both, live cell and fixed cell samples. This results in higher sensitivity, better quantitation from fluorescent signals, and longer tracking for time-course experiments.