CHIMERYS is a powerful tool for bottom-up proteomics data analysis, specifically for single-shot complete proteome DDA datasets. The algorithm is based on advanced artificial intelligence techniques, specifically deep learning, which allows it to effectively analyze and identify peptides in complex mixtures, even in chimeric spectra. By using CHIMERYS, researchers can expect to see a significant improvement in the identification rate and number of peptide identifications compared to traditional methods such as Sequest HT. The algorithm can double the identification rate and the number of peptide identifications, leading to a 1.5-fold increase in protein group identifications, higher sequence coverage, and more confident quantification.