Yes, absolutely! Speed keys work seamlessly with Lightroom. Both a Speed Key and Lightroom keyboard shortcut can be assigned to the same key. Both will continue to work as normal.  Tapping the Speed Key will activate the Lightroom keyboard shortcut as normal, but pressing and holding a Speed Key will activate the Speed Key function instead. For example, \\"Q\\" is assigned to Spot Removal. If you tap \\"Q\\", it will activate the Spot Removal tool as normal, however pressing and holding \\"Q\\" will activate the Speed Key's superpowers allowing you to adjust a slider!