Quantitating antioxidant activity in a biological sample provides vital information regarding the cells ability to combat damages induced by reactive oxygen species (ROS).
Our Amplite Colorimetric Antioxidant Assay Kit utilizes ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) as a colorimetric indicator to monitor antioxidant activity. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), ABTS is oxidized into the water soluble chromogen ABTS+. ABTS+ can be monitored spectrophotometrically at 405 nm. The absorbance suppressed in the test samples will be proportional to the concentrations of the antioxidants in those samples.