No, you can't. There are different ways to deal with this situation, especially when you accidentally purchased Bits. You can reach out to your credit card company or write to Twitch, but now, there is no official way to refund Twitch Bits. A resource I gathered from Twitch has this to say below.  Bits and Rewards have no monetary value (i.e., are not a cash account or equivalent), and do not constitute currency or property of any type. Bits and Rewards cannot be sold or transferred to other Users, and cannot be exchanged for cash or for any other goods and services. Users have no property, proprietary, intellectual property, ownership, or monetary interest in promotional or purchased Bits, which remain Twitch digital content subject to the Twitch Terms of Service, or in Rewards. Bits are non-refundable. We do not recognize the transfer of Bits or Rewards (including for money or any other consideration or items of value whether inside or outside of the Twitch Service). Accordingly, you may not purchase, sell, barter, or trade any Bits or Rewards, or offer to purchase, sell, or trade any Bits or Rewards. Any such attempted transfer will be null and void. – More details here.