Direct inoculation cultures can be directly added to wine after being rehydrated. Quick build-up cultures require a simple 12-24 hour rehydration step using an acclimated culture of Oenococcus oeni and an energizer/activator (included in kits). Standard cultures must go through a build-up protocol prior to inoculation of the wine. The standard build-up process can take from 5 to 20 days to prepare.