In order to non-invasively monitor pluripotency, you would first need to determine the signature secretory profile of your pluripotent stem cells. I’d suggest first assessing the secretory profile of your cells with the qualitative Proteome Profile XL Cytokine Array. You can compare your results with the data presented in the webinar, for which we show similar secretory profiles across iPS cell lines. As an alternate, there are pluripotent live-cell staining kits available that allow you to visually confirm pluripotency in live cells using fluorescence without out affecting proliferation or stemness.