The liquid attached to the outside of the tip is extra and not part of the set volume. To avoid picking up extra liquid on the outside of the tip, insert the pipette tip only 3-5 mm below liquid surface, or slightly deeper for very large volumes. Touch the tip on to the vessel wall after aspiration to remove excess liquid from outside the tip. Do not wipe the tip on a tissue cloth as it can contaminate the sample, or absorb liquid from inside the tip.
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