Yes and no. I do recommend adding a small amount to the dough to activate the yeast, but it's literally just a teaspoon. Since that is spread among the entire batch of cinnamon rolls, it's very minimal. Otherwise, you can use your favorite sweeteners in the filling and icing with some considerations. For example, you'll want to use a granulated sweetener for the filling to keep it from becoming too wet. Use whichever sweeteners work the best for your body!