The ingredients in these gummies (i.e., CBD, CBN, melatonin, and L-theanine) have no known interactions to suggest against use within a 24-hour time period. However, always consult your healthcare provider to be sure it is appropriate for your unique situation. Further, it's important to know that research is not yet clear on ideal CBD dosages for unique conditions and symptoms. When it comes to CBD, we like the statement, "Start low, go slow." With that in mind, even though the product serving may state "2 gummies," many folks begin with just one or even 1/2 of one gummy. In those cases, your healthcare provider may support beginning with 1/2-1 calm gummy in the morning and 1/2-1 sleep gummy at night. We also like to use melatonin as a more infrequent, but as-needed tool to assist with falling asleep or as a temporary support to reset the internal clock (read more about that here: https://www.naturalmentalhealth.com/blog/melatonin-for-sleep). Our Sleep gummies are ideal for those applications as well. And in general, when it comes to CBD (without melatonin) we find that some folks find support with daily use, whereas others simply take as-needed. Again, check with your healthcare provider and then pay close attention to your own unique needs.