We recommend using ROS Brite 700 (Cat# 16004) for in vivo imaging in small animals.
The concentration of ROS Brite 700 required varies between different cell lines, we recommend testing with different concentrations to determine the optimal dosage. Foersch and colleagues used ROS Brite 700 for in vivo imaging in mice (Foersch et al. 2015). They administered a final concentration of 10 μg/g body weight, intravenously, and waited 20 minutes before examining.
For a sample protocol and additional publications using ROS Brite 700, see links below in Resources.