We've optimized each assay for the cell number stated in the protocol. You can try to scale down and decrease the amount of buffers used proportionally, but it may not fall within the range for the kit. This is why we encourage users to follow the sample recommendation in the protocol - if, for whatever reason, this isn't possible, we would recommend harvesting cells at different times and freezing them until there's enough sample to proceed with the protocol (this is not appropriate for assays to be run on live or fixed cells).