Before first use we recommend removing the VitaBead Diffuser and rinsing it for 20 seconds. Then soak it in warm water for 20 minutes, followed by letting it air dry completely overnight. Wash the bottle separately with warm water and plant-based soap if desired. Once dry, insert the Diffuser back into the bottle and you're ready to go! Please Note: Because of the living Nature of the VitaBeads, there will be pressure buildup within the DYLN Bottle. You may receive an occasional refreshing mist of water while opening. During the first two weeks of use, be sure to loosen the cap every few hours to release the pressure. Repeat these instructions each time you replace with a new Diffuser.