There are many differences between Enzymes and Hormones, some of them being:Enzymes are biological catalysts that change in their composition and they help in increasing the pace of a reaction or function. Hormones are molecules that trigger a function to happen inside the body.Enzymes act only on one particular site whereas the Hormones are flexible and can be formed in more than one site. Hormones have the mechanism to diffuse inside a cell membrane, but enzymes do not have this mechanism.  Enzymes and hormones majorly differ in their chemical composition. This chemical make-up changes after use in hormones but remains the same in enzymes.