To provide precise and trustworthy readings in the lab, a micropipette must be calibrated. To ensure that the volume of liquid dispensed by the micropipette is equal to the predetermined volume, adjustments must be made to it. For optimal accuracy, especially in regulated contexts, professional calibration services are advised; nevertheless, basic in-house calibration can be accomplished with particular procedures. Equipment for calibration 1. A high-precision analytical balance that can measure small weights accurately, preferably to four decimal places (0.0001 g). 2. Distilled or deionized water, as it has a known density (1 g/mL at room temperature), making calculations straightforward. 3. A clean, dry beaker or container for wastewater. 4. Appropriate-sized micropipette tips. 5. Gloves and lab coats for personal protection.