Vo filme Duna hrajú Kyle MacLachlan (Paul Atreides), Francesca Annis (Lady Jessica), Patrick Stewart (Gurney Halleck), Linda Hunt (Shadout Mapes), José Ferrer (Padisha Emperor Shaddam IV), Freddie Jones (Thufir Hawat), Brad Dourif (Piter De Vries), Richard Jordan (Duncan Idaho), Virginia Madsen (Princess Irulan), Silvana Mangano (Reverend Mother Ramallo), Everett McGill (Stilgar), Sting (Feyd Rautha), Kenneth McMillan (Baron Vladimir Harkonnen), Jack Nance (Nefud), Siân Phillips (Reverend Mother Gaius Helen Mohiam), Jürgen Prochnow (Duke Leto Atreides), Leonardo Cimino (The Baron's Doctor), Paul L. Smith (The Beast Rabban), Dean Stockwell (Doctor Wellington Yueh), Max von Sydow (Doctor Kynes), Alicia Witt (Alia), Sean Young (Chani), Danny Corkill (Orlop), Honorato Magaloni (Otheym), Judd Omen (Jamis), Molly Wryn (Harah), Angélica Aragón (Bene Gesserit Sister (uncredited)), Thomas Ebert (House Atreides Guard (uncredited)), Humberto Elizondo (Czigo (uncredited)), Ricardo Hill ((uncredited)), Ernesto Laguardia (Harkonnen's Victim (uncredited)), David Lynch (Spice Miner (uncredited)), Ramón Menéndez (Kinet (uncredited)), Ana Ofelia Murguía (Palace Maid (uncredited)), Claudia Ramírez (Fremen Girl (uncredited)), Julieta Rosen (Palace Maid (uncredited)), John Sabol (Fedaykin Fighter (uncredited)), Margarita Sanz (Lady Jessica's Maid (uncredited)), Jacqueline Voltaire ((uncredited)), José Luis García Mainou ((uncredited)).