1,2,3,4. The first step in the DNA replication process is relax. The supercoil is relaxed by the enzyme DNA gyrase (also called topoisomerase II). The second step in DNA replication is unwind. After the supercoil is relaxed, the enzyme DNA helicase unwinds the two strands.: The third step in DNA replication is replicate. The enzyme DNA polymerase adds the precursor bases to replicate the original DNA and form new DNA strands. The fourth step is migrate. The two newly formed strands are interlocked until the enzyme topoisomerase IV frees them to migrate to opposite sides of the cell, where they are segregated into the two daughter cells. More test answers on https://studyhippo.com/nursing-pharm-ch52-fluoroquinolones-and-miscellaneous-antibacterials/