No, different cooking methods do not generally affect the protein content of foods. Proteins are composed of complex structures that are resistant to significant changes during cooking. While cooking methods can impact the texture, taste, and overall quality of protein-rich foods, they typically do not alter the protein content itself. However, it is important to note that excessive heat, prolonged cooking times, or cooking at very high temperatures can lead to some protein denaturation or degradation. To maximize protein retention, it is recommended to choose cooking methods that are gentle, such as steaming, boiling, baking, or poaching, and to avoid excessive cooking times or high temperatures. Proper handling and cooking techniques are crucial to maintain the nutritional value of protein-rich foods.