You don't need to wear mascara to wear the LashLiner Lashes or keep them on. However, we suggest that you put a layer of mascara on your natural lashes prior to putting on your LashLiner Lashes. Although it is not needed it does look nice because it blends the color of your natural lashes to the color of the LashLiner Lashes. We do not recommend that you put mascara on the Cooco lashes.