To determine the effect of nicotine on continuous attention, working memory and computational processing, a study composed of tobacco deprived and non-deprived smokers was carried out. They used 28 smokers in a double-blind, placebo controlled, within-subject (all participants take part in every condition) study where the subjects were tobacco deprived overnight for 12 hours at one session and smoked as much as desired before the other. Each participant was treated with nicotine in the form of a nasal spray at 90 minute intervals at a strength of 0mg, 1mg and 2mg in random order. Before and after each dose, blood samples were taken and a variety of tests applied.