Exosomes are small vesicles obtained from cells and are usually produced in a laboratory environment. These vesicles play an important role in intercellular communication and also play a critical function in biological processes and are obtained by growing stem cells in a culture medium. While stem cells are multiplied under controlled conditions in special culture dishes; a healthy environment is created by providing certain growth factors and nutrients. Exosomes are isolated from the liquid medium in which these cells are located and then prepared for use in various research and treatment methods. This process is critical for better understanding the properties of exosomes and their interactions between cells.