According to protocol, the tube can be store between -20°C to -70°C in order to stabilize the RNA for longer period of time. Alternatively, the end-user can aliquot the small volume of homogeneous mixture (after sample collection and mixing) from the stool tube to microcentrifuge tube and then store at -80°C. This aliquoting method also prevents the multiple freeze and thaw cycles that may impact on RNA quality.