Vo filme Bohemian Rhapsody hrajú Rami Malek (Freddie Mercury), Gwilym Lee (Brian May), Ben Hardy (Roger Taylor), Joseph Mazzello (John Deacon), Lucy Boynton (Mary Austin), Aidan Gillen (John Reid), Allen Leech (Paul Prenter), Tom Hollander (Jim Beach), Mike Myers (Ray Foster), Aaron McCusker (Jim Hutton), Meneka Das (Jer Bulsara), Ace Bhatti (Bomi Bulsara), Priya Blackburn (Kashmira Bulsara), Max Bennett (David), Dermot Murphy (Bob Geldof), Dickie Beau (Kenny Everett), Jack Roth (Tim Staffell), Neil Fox-Roberts (Mr. Austin), Jess Radomska (Cheryl), Michelle Duncan (Shelley Stern), Ross Green (Reporter 1), Bruce Mackinnon (Reporter 2), Joshua Higgott (Reporter 3), Pat Lally (Reporter 4), William Owen (Reporter 5), Philip Andrew (Reinhold Mack), Tim Plester (RT Baker), Felipe Bejarano (Brazilian Crewman), Kieran Hardcastle (Heathrow Baggage Handler), Martin Oelbermann (German TV Journalist), Ian Gabriel Dumdum (Hospital Doctor), Matt Greenwood (Young Man at Clinic), Royce Cronin (TV Director), James MacLaren (A & R Man), Andrew Bowerman (Smile Audience Member), Drew P. (Live Aid Organiser), Haf Gibson (Roger's Girlfriend 1), Honor Hellon (Roger's Girlfriend 2), Rosy Benjamin (Dominique), Leila Crerar (Chrissie), Katherine Newman (Veronica), Adam Rauf (Young Farrokh Bulsara), Peter Howe (Engineer at Studio), John Ottman (Live TV Director), James Wallace (Technical Director), Matthew Houston (Larry Mullen Jr. (uncredited)), Scott Morrison Watson (Steve (uncredited)), Devlin Lloyd (Floor Manager (uncredited)), Stefan Kopiecki (Video Producer (uncredited)), Garry Summers (Voice Doctor (uncredited)), Matthew Fredricks (Freddie's Lover (uncredited)), Ian Jareth Williamson (Freddie's Former Lover (uncredited)), Adam Lazarus (Raver (uncredited)), Johanna Thea (VIP Party Guest (uncredited)), Adam Lambert (Man at Truck Stop (uncredited)), Andreea Helen David (Live Aid Volunteer (uncredited)), Jason Lines (Munich Guitarist (uncredited)), Adam James Johnston (Adam Clayton (uncredited)), Freddie Mercury (Self (archive footage) (uncredited)), Brian May (Self (archive footage) (uncredited)), Roger Taylor (Self (archive footage) (uncredited)), John Deacon (Self (archive footage) (uncredited)).