It depends on the biomarkers of interests. The most
common situation where a substance would interfere with an immuneassay is that the substance
might chelate the protein of interest and form a comple, shielding the epitope detected by
the antibody thus blocking the reaction. The typical substances that bear such risks are
anti-coagulents, such as Heparin, EDTA, citrate acid, SDS, used in collecting blood. Each
antibody pair/ELISA kit typically informs you which anti-coagulent(s) to avoid. If you have
any biomarkers in question feel free to ask us any time. If it is a custom assay you are
developing with us, we will figure this out for you.