When the follicles grow to maturity, it is necessary to make a special injection to introduce a trigger for the final egg maturation. Follicle puncture and egg production are carried out in 34-38 hours after the trigger introduction. Only after this step, it is possible to obtain mature eggs.The procedure is performed under general anesthesia and is monitored by ultrasound. The doctor uses a special needle to collect follicular fluid. After that, the embryologist selects eggs for further fertilization. The more eggs you get, the better result you can achieve.The obtained eggs and sperm cells are placed in a special incubator where the temperature is 37°C and humidity is high. In these conditions the fertilization takes place.In some cases, embryologists apply an intracytoplasmic sperm injection (ICSI). Using this technique, the embryologist selects the most qualitative spermatozoon and implants it into the egg cytoplasm. The procedure is performed if the quality of the ejaculate is low or the frozen eggs are used.