After each stage of the STEM method, we characterize the Fabs using multiple methods:
ELISA
Octet Bio-Layer Interferometry (BLI)
Flow cytometry
Functional Assays
Full sequence analysis
Surface plasmon resonance (SPR)
Our dual-purpose vector allows soluble Fab expression in 96-well format, so we can screen lead candidates in high-throughput using crude bacterial supernatant before proceeding to the expensive, time-consuming IgG stage.