Yes, the Shuttle Express is Free with any paid Busch Gardens Ticket. Daily round-trip transportation from Orlando to Busch Gardens Tampa (just a short 1-hour ride) is offered from select locations in Orlando. Reservations are recommended by visiting Buschgardens.mears.com for more information.
Pick-up locations:
8:30am Doubletree Orlando by Hilton at the entrance to Universal Orlando - 5780 Major Blvd.
8:45am Icon Park - 8350 Universal Blvd.
9am SeaWorld - 7007 SeaWorld Drive - Bus Parking
9:15am Publix - 8145 Vineland Avenue
9:30am Maingate Lakeside Resort - 7769 West Highway 192, Kissimmee in front parking lot.
Departure: The bus departs Busch Gardens Tampa at the park close or 7pm during the peak seasons.