It's perfectly fine to move your facial muscles right after the injection. In fact, it has been reported that moving the muscles may increase the release of a neurotransmitter called acetylcholine, which can enhance the absorption of Botulinum Toxin into the moved muscles, potentially increasing its effectiveness8. In our clinic, we recommend deliberately moving the facial muscles in the treatment area to create some wrinkles within three hours after the Botulinum Toxin injection.