The RY785/GxTX pharmacology could be a valuable tool for investigating the developmental or activity-dependent regulation of Kv2/KvS channel composition in native neurons. By using this pharmacological approach to selectively target Kv2/KvS channels and Kv2-only channels, researchers can study the changes in channel composition under different conditions. For example, during development, the expression levels of KvS subunits may change, leading to alterations in the proportion of Kv2/KvS channels in specific cell types. Similarly, activity-dependent regulation of KvS subunits could impact the functional properties of Kv2 channels in response to neuronal activity. By applying the RY785/GxTX approach in native neurons at different developmental stages or under various activity conditions, researchers can elucidate the dynamic regulation of Kv2/KvS channel composition and its implications for neuronal function.