To cultivate successful mycelium growth, it is crucial to provide an ideal environment for your bag. Find a cool and shaded area where the temperature ranges between 55 to 75 degrees Fahrenheit (depending on strain). Give the bag a a few days to colonize and observe the emergence of mycelium on the substrate. As the mycelium grows from the injection point, it will gradually spread outwards, forming a white layer on the substrate. Once the mycelium has extended three to four inches in length, it is time to carefully break up the colonized substrate and blend it with the uncolonized substrate. This will facilitate the colonization process and quicken its growth. The colonization process happens quickly in our Super Grow Bags. Keep an eye on the bag and watch for the complete white layer of mycelium to form, indicating that your spawn is ready for an air transfer.