Any format of culture vessel (e.g. flask, 6-well plates, etc) can be used. Seed cells at a density of 5 x 10⁴ cells/cm². Depending on the surface area of your culture vessel, calculate how many cells you will need to have on hand. Example: For one 60 mm dish, you will need 5 x 10⁴ cells/cm² x 20 cm² = 1 x 10⁶ cells total.