HDX can offer faster initial results compared to cryo-EM, particularly when preliminary work involving vitrification conditions and grid screening needs to be done. However, once these conditions are established, the turnaround times for both techniques can become similar.
Cryo-EM can still be considered a “fast” technique, as we are able to provide results in 2 weeks or less from sample receipt.
It's important to note that HDX provides "low-resolution" details, primarily revealing the general area of interactions. In contrast, cryo-EM has the capability to provide specific atomic interactions through a ~3.5 Angstrom resolution map.
When it comes to FABs binding to the same antigen simultaneously, the more, the better. We can get epitopes for all FABs in one experiment. Additionally, the added mass will make the particles easier to pick and to align, thus increasing the possibility of achieving higher resolution. Learn more about the differences between techniques here.
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