Lean, tender cuts of meat are best for making homemade Beef Jerky in a Dehydrator. Ask your butcher if you're not sure what that looks like. In the past, I've had great success with the following cuts: Brisket, Rouladen, Top or Bottom Round, Eye of Round, Sirloin Tip or Flank Steak. Either which way you go, the important part to remember is to make sure the beef is thinly sliced.