SynergyFinder is generally used for small-scale combination studies. The advantage is the determination of synergy according to curve shift analysis, CI Index and isobolograms; it is based on full dose response curves of both single agents and 3 mixtures per combination (ratios 1:4; 1:1; 4:1). Literature references are: Uitdehaag et al. (2014); Straetemans et al. (2005) and Chou (2010).
SynergyScreen is generally used for broad combination screening. Advantage: less laborious and therefore quicker and lower price. It concerns determination of synergy according to curve shift analysis; based on full dose response curve of 1 single compound and mixture curve of that compound with a fixed concentration of the 2nd compound.