Using a semi-auto analyzer, there are two methods to do the reaction, one is the flow cell method, the other is the cuvette. For the former, it refers to the chemistry reaction of the same test sample and reagent mixed in the same pipeline flow process. This is the first generation of automatic biochemistry analyzer. For the latter, it refers to the chemistry reaction of each sample to be tested and reagent to be mixed in their respective reaction cups or cuvettes. For the flow cell method, it makes the detection more accurate with its unique optical design, but for the long-term use, sample testing will be influenced by tube pulution. For cuvettes, because of Independent units of reaction, it makes the result more reliable.