An extensively employed application of mitochondrial DNA (mtDNA) in DNA testing is the determination of ancestry. Due to its relatively slower rate of change compared to nuclear DNA and its exclusive maternal lineage, mtDNA offers a clearer historical record, specifically of maternal ancestry. This is attributed to the fact that it remains unaltered by paternal DNA. The analysis of mtDNA has played a pivotal role in allowing scientists to trace the maternal ancestry of the entire human population back to East Africa, with the identified time frame falling approximately between 100,000 and 200,000 years ago. In contrast to regular DNA testing, mtDNA testing can reveal certain aspects of ancestry that may not be readily apparent. For individuals without a scientific background, mtDNA serves as compelling evidence of relationships among those sharing the same maternal lineage. The shared nature of mtDNA among individuals with common maternal ancestry provides clear confirmation of connections extending from mothers to grandmothers, great-grandmothers, and so forth.