The MISSION TRC shRNA library is composed of three formats to give you flexibility and convenience. The purified plasmid DNA format may be used directly for transient transfection assays or used with our lentiviral packaging mix (Product No. SHP001) to produce lentiviral particles. You may further propogate the DNA by transforming the plasmids into competent cells (we recommend GC5 cells) to create bacterial glycerol stocks.
The lentiviral particles offer the most convenience. They may be added directly to cells for stable transduction. You are correct; the cells that are transduced will continue to express the integrated shRNA. The cells may be expanded for many downstream assays.