The bass clef sign is positioned so that the two clef dots are on either side of the staff's second-highest line: As a result, the second line from the top is F3: Middle C (C4) is positioned on the first ledger line above the staff in bass clef (the inverse of treble clef): G3. The bass clef helps to show where the bass notes should be played by indicating which lines or spaces they should occupy. In general, the lower the note is on the stave, the higher it should be played. So, a bass note on string 4 would be played near the middle of the piano. Notes on strings 1 and 2 would be played lower down the keyboard than string 4, while notes on strings 3 and 5 would be even lower down. It is important to remember that, on the bass clef, the lowest note available on the instrument is G. This means that, if you were to write out all the possible notes within the range of the bass guitar, it would list them from G to Bb (or Ab). Notes that cross between clefs are generally notated with a small dot below the note to indicate that the pitch should be played on the bass clef. For example, if you were to play A on the bass guitar, this would be written as A. Bassists often use drumsticks to play the low notes on their instrument.