Amino acids are often difficult to solubilize. A common technique is to use 1 molar equivalent (1eq) of sodium hydroxide (NaOH). To dissolve compound in 1 equivalent of NaOH(aq.), the following formula can be used to calculate the volume of NaOH (aq) (VolNaOH) of concentration (ConcNaOH ) needed:
E.g for a product with a molecular weight of 197.13g, a mass of 5 mg, and using 100 mM NaOH: