Glycerine is a good hydrating agent and prevents the leaf peel from crumpling and drying. An additional benefit of using glycerine is its refractive property as it reflects light better for our observation. The peel appears clearer, shinier, and clearly observed. Glycerin possesses the same concentration as the cell sap due to which the cells in the leaf peel mounted on the slide will neither be flaccid or turgid and can be observed in their natural form.