No to both fresh and frozen pineapple when making this recipe. The enzymes in fresh pineapple and also in frozen pineapple will actually prevent the gelatin in the jello from setting up.u003cbr/u003eThis means lots of runny disappointment instead of lovely dessert. Stick with canned pineapple for success with this recipe.u003cbr/u003eThe only way to use fresh pineapple is to toss in the pieces of fresh or frozen pineapple when you boil the liquid for preparing the jello. This heating process will deactivate those enzymes.