The termination in the prokaryotic transcription can be Rho-dependent and Rho-independent. In the Rho-dependent termination mechanism, the protein Rho binds to the ribosome-free mRNA and translocates to the RNA polymerase. By the activity of the helicase, the Rho protein unwinds the RNA-DNA complex and mRNA is being released which marks the termination of transcription. In the Rho-independent mechanism, the information in the DNA gives rise to RNA sequence which has the potential to form secondary structures(hairpin loops) in transcribed RNA. The formation of hairpin loops causes the separation of the mRNA and RNA polymerase and mRNA is being released.