Presence of ammonia in a wastewater treatment plant can lead to toxicity. The concentration of ammonia can be reduced by secondary treatment of wastewater with microbial action where the ammonia is first oxidized to nitrites and nitrates, this process is known as nitrification. Further, nitrates are reduced to nitrogen gas which is released into the atmosphere, this process is known as denitrification. Denitrification is especially crucial since the release of nitrates into the environment can cause eutrophication or algal bloom.