Inoculating a Mushroom Growing Kit injection port grain bag with a liquid culture syringe is a common method for growing mushrooms. The following is a brief description of the process: Start by sterilizing your work area, your hands, and all equipment you will be using. This will help prevent contamination. Shake the liquid culture syringe to distribute the mycelium evenly throughout the liquid. Locate the injection port on the grain bag. This is usually a small self-healing rubber port on the side of the bag. Wipe the injection port with an alcohol swab to sterilize it. Remove the cap from the syringe and insert the needle into the injection port at a slight angle. Be careful not to push the needle in too far, as this could damage the bag. Slowly depress the plunger on the syringe to inject the liquid culture into the bag. Try to distribute the culture evenly throughout the grain. Withdraw the needle and replace the cap on the syringe. Repeat this process with any other bags you want to inoculate. Be sure to sterilize the needle in between inoculating bags. Place the inoculated bags in a warm, dark place and wait for the mycelium to grow. If you have a still air box or flow hood, that would be optimal for minimizing contamination. Depending on the species of mushroom, colonization can take anywhere from a few days to a few weeks. By following these steps, you should be able to successfully inoculate an injection port grain bag with a liquid culture syringe and begin growing your own mushrooms. For best results be sure your grain bag is fully colonized before transfer.