Based on primer-genome sequence alignments, our kits can detect at least 110 species belonging to the Mollicutes class (Acholeplasma, Ureaplasma, Spiroplasma, Mycoplasma) of about 165 species which are mentioned in the literature, so far. For many – less common – species, however, there is no reliable data or sequence information. Thus, we cannot make any statement about the kit specificity for these species. Importantly, we can detect all species mentioned in publications as significant cell culture contaminants and many others.