Vo filme Madagaskar 2: Útek do Afriky hrajú Ben Stiller (Alex (voice)), Chris Rock (Marty / Additional Zebras (voice)), David Schwimmer (Melman (voice)), Jada Pinkett Smith (Gloria (voice)), Sacha Baron Cohen (Julien (voice)), Cedric the Entertainer (Maurice (voice)), Andy Richter (Mort (voice)), Bernie Mac (Zuba (voice)), Alec Baldwin (Makunga (voice)), Sherri Shepherd (Mom (voice)), Will.i.am (Moto Moto (voice)), Elisa Gabrielli (Nana (voice)), Tom McGrath (Skipper / Lemur (voice)), Chris Miller (Kowalski (voice)), Christopher Knights (Private (voice)), Conrad Vernon (Mason (voice)), Quinn Dempsey Stiller (Baby Alex (voice)), Declan Swift (Baby Alex (voice)), Fred Tatasciore (Teetsi / Poacher #1 / Elephant (voice)), Eric Darnell (Joe the Witch Doctor / Poacher #2 (voice)), Willow Smith (Baby Glorida (voice)), Thomas Stanley (Baby Marty (voice)), Zachary Gordon (Baby Melman (voice)), Meredith Vieira (Newscaster (voice)), Lesley Stahl (Newscaster (voice)), Al Roker (Newscaster (voice)), David Soren (Lemur (voice)), Phil LaMarr (Guide (voice)), Stephen Kearin (Stephen the Giraffe / Tourist with Video Camera / Rhino (voice)), Dan O'Connor (Tourist with University Shirt / Cape Buffalo (voice)), Edie Mirman (Telephone Recording (voice)), Fergie (Hippo Girlfriend (voice)), Harland Williams (Additional Giraffe (voice)), Danny Jacobs (Tourist with New York T-Shirt (voice)), Bridget Hoffman (Female Tourist (voice)), Terrence Hardy Jr. (Cub (voice)), Conner Rayburn (Little Giraffe (voice)), Holly Dorff (Fish (voice)), David P. Smith (Bobby the Dik Dik (voice)), Lynnanne Zager (Lioness (voice)), Jackie Gonneau (Additional Dik Dik (voice)).