One T-175 flask yields approximately 500 µL of VLPs following resuspension. VLP protein concentration depends on protein expression and purification efficiency, and may be between 0.1 µg/µL and 1 µg/µL. Depending on the efficiency of expression of your GPCR and the activity of the GPCR for your ligand of interest, a single reaction may generate sufficient VLP sample for up to 1000 ligand binding assay data points.