The Oris kits are configured for use in a single experiment. For Oris, the stoppers must be stored refrigerated until use to preserve the perfect structural fit that generates the reproducible, tightly bounded exclusion zone. For OrisPro, the humidity in the incubator hydrates the gel in the unused wells, which will create variability in exclusion zone size and gel dissolution time in subsequent experiments. If you need fewer than 96 wells per experiment, consider the Oris Cell Migration Assembly Kit – FLEX, which includes four 96-well plates and four packs of 24 stoppers. Use each plate only once, with any number of stoppers that is a multiple of four.