Our top tips:
Avoid foaming or bubbles when mixing or reconstituting components.
Avoid cross-contamination of samples or reagents by changing tips between sample, standard and reagent additions.
Ensure plates are properly sealed or covered during incubation steps.
Ensure all reagents and solutions are at the appropriate temperature for use before starting the assay (i.e., not taken straight from the ice bucket into the assay), and mix reagents gently before use.
Don’t allow reagents to sit for extended periods before use, or reuse prepared reagents, always prepare fresh reaction mix before use.
Make sure that all equipment is switched on and set at the appropriate temperature before starting the assay.