If you are growing cells on cover slips to do immunocytochemistry, we recommend using Permanox chamber slides:
Permanox chamber slides, 4-well, pk/16 (VWR, Cat. No. 62407-330)
Permanox chamber slides, 8-well, pk/16 (VWR, Cat. No. 62407-335)
If you are growing cells on cover slips for other purposes and require coating, use this procedure to double coat the cover slips:
Incubate cover slips overnight at room temperature in poly-ornithine solution (final concentration = 100 mg/ml in cell culture water)
Rinse twice with sterile water
Incubate cover slips overnight at 4℃ in laminin solution (final concentration = 15 mg/ml in cell culture water)