The most commonly used and commercially available synthetic substrate is Synthemax by Corning. This substrate has been used along with Essential 8 medium by many scientists. These are the following evaluations:
1. Test for pluripotency after culturing cells for a long period of time (50 passages or more).
2. Test for the ability to form Embryoid Bodies and potential for tri-lineage differentiation
3. Test for normal karyotype after long-term use of Synthemax
4. Test for the ability of the cells to differentiate into the cells of your interest