A Barley yellow dwarf virus was detected in wheat (Triticum aestivum L.) expressing symptoms of yellowing and reddening of leaves, leaf chlorosis and general plant stunting. Diseased plants were collected at the Plant Breeding Centre of Lithuanian Institute of Agriculture in the breeding-experimental fields (Kėdainiai region) and wheat fields of Kaunas region. The electron microscopy investigation of leaf extracts revealed the presence of particles measuring 25 nm in diameter (negatively stained). The presence of Barley yellow dwarf virus in diseased wheat was demonstrated using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR).
Keywords: Cereal crops, BYDV, DAS-ELISA, RT-PCR.