Renal biopsy, a safe medical procedure, has become increasingly meaningful in the diagnosis of medical conditions. Presently, a spring-loaded automatic cutting needle is occasionally used for taking abdominal renal tissue. Adults sometimes use 14-or 16-gauge needles for subcutaneous injections in children up to 8 years of age, where the normal glomerulus diameter is approximately 200-250 microns. However, children between the ages of 8 and 10 years should use finer 18-gauge needles depending on the glomerular diameter. If possible, 2-3 cores of material taken by renal biopsy shall be for light microscopy (LM); for another, immunofluorescence (IF); and for the third, electron microscopy (EM), if required. Each renal biopsy core should be enclosed in a clear plastic bag that is marked with the necessary information (e.g. Light microscopy, Ultrastructure). Add an appropriate fixative to the case containing the biopsy cores before sending it to the pathology lab. Biopsy specimens in saline that have to be cultivated within 6 hours are best treated in this way. If time travel is not an option, a 2-in-1 protocol may be implemented: With direct transmission electron microscopy, entire core could be useful for EM; with boundless/partial core, one third of core was a fragment in the iris film for IF, while the rest of cortical cerebrum was placed in the transportation medium for LM. The renal pathologist typically works closely with nephrologists and kidney surgeons. Given that lacking the right clinical information severely hampers more detailed evaluation of a renal biopsy, such procedures ideally include adequate diagnostic information. An extensive medical history, including previous medical problems, recent laboratory results focusing on urinalysis, biochemical parameters (urine urea, creatinine, total protein, cholesterol), serological investigations (anti-nuclear antibody, double-stranded DNA antibody, anti-glomerular basement membrane antibody, hepatitis B antibody, hepatitis C antibody, human immunodeficiency virus antibody), and other elements of interest should be detailed.