The speed of the separation protocol can influence the quantity and quality of the cells you are able to isolate, which in turn may affect reproducibility. Additionally, the longer it takes to complete the cell isolation protocol, the fewer samples you can process and less time you have for other lab tasks.
To maximize efficiency and throughput, a rapid and easy protocol is recommended. Buoyancy Activated Cell Separation (BACS) represents one of the quickest and easiest cell separation methods.