Yes, you will have to pay taxes on your winnings from online sports betting in Florida. Any income you earn, including gambling winnings, is subject to federal and state taxes. The exact amount of taxes you will owe depends on your tax bracket and the amount of winnings you receive. It is important to keep track of all your gambling winnings and losses and report them accurately on your tax return. Failure to report gambling winnings can result in penalties and interest charges. It is recommended that you consult with a tax professional to ensure proper reporting of your gambling winnings.