The number of Jello shots that can be made from one box of Jello gelatin depends on the size of the Jello shot cups used and the amount of alcohol added to the mixture. As a general rule, one 3-ounce box of Jello gelatin, when combined with 1 cup of boiling water and 1 cup of alcohol, can make approximately 10 to 12 Jello shots in standard 2-ounce cups.