Yes! I do it all the times! I double or triple the measurements, refrigerate some for immediate use and freeze the rest. I pour it into ice cube molds and once the pesto i frozen I remove them from the molds and place the pesto cubes into a freezer bag and into the freezer again. This way, whenever I need to make a pasta dish - for instance, I only let thaw 2-3 cubes or as many as I need.