Microscopy image showing excessive red blood cells in a cancer cell sample (green cells).
When peripheral blood is harvested and cleaned up with a combination of density gradient and microfluidics, the sample may still contain over 90% RBCs. This is a problem.
In fact, in our conversations with researchers, we have heard firsthand how red blood cells get in the way:
Lower loading efficiency for single-cell genomics: When excessive RBCs are present, they reduce the number of wells loaded on sequencing systems.
Limits the integration of microfluidics: Labs are working to use new microfluidic system as a bulk enrichment step. However, excessive RBCs can still be present and reduce the ability to perform downstream testing.
Physically blocks culture: When working to culture rare cells, RBCs take up precious real estate on the plate, which can physically limit the growth of rare cells.