Yes, you can play sports with your Invisalign aligners in, but it's crucial to wear a mouthguard to protect your teeth and aligners. Invisalign aligners are made of durable plastic, but they can be damaged or dislodged if you experience a strong impact on the mouth. A mouthguard can help prevent injuries to your teeth, lips, and tongue, as well as protect your aligners from damage during sports activities.