The USPTO has established size limits for Sequence Listing submissions via their patent electronic filing system: The maximum file size for Sequence Listing and CRF text files is 100 megabytes. Files submitted electronically cannot be compressed. If a Sequence Listing or CRF text file exceeds 100 megabytes, it must be filed on read-only optical disc(s). The MPEP states: One hundred (100) megabytes is the size limit for "Sequence Listing" and CRF text files submitted via the USPTO patent electronic filing system, and "Sequence Listing" and CRF text files cannot be compressed when submitted via the USPTO patent electronic filing system. If a user wishes to submit an electronic copy of a "Sequence Listing" or CRF text file that exceeds 100 megabytes, the "Sequence Listing" or CRF must be filed on read-only optical disc(s). It's important to note that larger files may be subject to additional fees and handling requirements, particularly for files 300 MB or larger. To learn more: Sequence Listing USPTO File Size Limits Electronic Filing