Option 1) Hind II  Option 2) Protease  Option 3) DNase I  Option 4) RNase Answer: Option 1) Hind II Restriction Endonuclease - These enzymes cleave DNA only within or near the specific base sequence. These sequences are called recognition sites. They are of three types RE- I, RE-II and RE-III. wherein 1st Restriction enzyme (Hind II) was used in RDT.   Hind II are restriction endonucleases because they cleave DNA at specific sequences within the DNA strand.