Peptidic FRET (Fluorescence Resonance Energy Transfer) substrates contain a donor (a fluorophore) and an acceptor (quencher) which absorbs at the emission wavelength of the fluorophore.
The quencher absorbs the energy emitted by the fluorophore only as long as they are in close proximity, connected by the peptide chain. Enzymatic hydrolysis causes an increase of fluorescence irrespective of the cleavage position, provided that donor and acceptor are disconnected.
Bachem offers a large choice of FRET substrates for MMPs, ACE, β-secretase and other enzymes. For an overview of our products you can download our product monograph “FRET Substrates”.