We would love to give you a definitive answer but as our bodies are so unique, we can’t give an exact timeline. Based on our studies and customer results, some individuals will see instant benefits from the first night and others after a few days/weeks - in both cases sleep will gradually improve over weeks and months. Please also bear in mind that the benefits will increase over time, as the body accumulates certain nutrients. Ten PM is not a sedative, but a healthy long term solution to better sleep. Please also make sure to follow the sleep tips in the "Guide On How To Eliminate Stress & Anxiety Before Bedtime" which you will have receive for free as part of your purchase of one of our products. Following these tips while using our products will see the best results