If the protein does not perform well in downstream applications for endotoxin removal, it may be because a different Elution Buffer was used. The provided Elution Buffer G has been optimized for endotoxin-free recoveries. The endotoxin-free properties of the eluted protein will be compromised if another Elution Buffer G is used. If a different Elution Buffer G other than the one provided is used, the buffer should also be checked for any components that may interfere with the application. Common components that are known to interfere are high salts, detergents, and other denaturants. Check the compatibility of your Elution Buffer with the intended use.