Usually tacrolimus is taken twice a day, 12 hours apart, such as at 8 a.m. and 8 p.m. Occasionally it is taken only once a day (usually in the morning and at the same time each day) or as often as three times a day (8 hours apart, such as at 7 a.m., 3 p.m., and 11 p.m.). Tacrolimus can be taken with or without food.