No, ADO.NET is primarily designed for working with relational databases, such as SQL Server, MySQL, and Oracle. It utilizes data providers specific to these databases, making it unsuitable for non-relational databases like MongoDB or data sources that don't follow the relational model. To work with non-relational databases, one would need to use other technologies or frameworks that support their respective data models, like MongoDB's .NET driver or APIs for other NoSQL databases.