Yes, before copying the files of data to your backup disk, Vimalin will create a signature of your data file.
This signature is then kept in a database so that it can always be used to verify.
In fact when you restore a Virtual Machine, Vimalin will verify each copied file against that signature to make sure every bit is still the same.
Vimalin uses industry standard MD5 sum hash signatures.
If needed, you can verify the files by hand by looking at the job.metadata file in your backup folder. Note that this file is only kept at the backup folder, not copied to your VM when you happen to restore the virtual machine.