Yes, you can dispute a hotel reservation charge if you believe it's incorrect or if the hotel changed the rate after booking without proper justification. First, try discussing the issue with the hotel to see if they're willing to resolve the matter amicably. If that doesn't work, you can escalate the dispute to the booking platform you used (if applicable) or contact your credit card issuer to dispute the charge. Keep in mind that you'll need evidence of the original rate and any terms and conditions that support your claim.