In any LC-MS testing method, the required sample volume depends on multiple factors such as calibration range, compound sensitivity, chromatography column, matrix-based suppression or enhancement, mobile phase selection, and detector linearity. Often, LC-MS method sample volume requirement can vary between 2 µL-200 µL. We can use specialized systems such as capillary flow for shallow volumes. Our scientists recommend collecting additional sample volume in pharmacokinetic/toxicokinetic assessments from biological samples to complete reassay or incurred sample reanalysis (ISR) to support your LC-MS method validation.