The EK0411 Mouse IL-6 ELISA Kit have been tested in liver tissue lysate but we regret to say that we do not have any related information on brain lysate. But if you want to test this EK0411 Mouse IL-6 ELISA Kit on brain tissue lysate we strongly recommend diluting the samples at least 5-fold with assay diluent to minimized any effects of the lysis buffer and the samples may need to be diluted further but you have to determined it.