No. The acetone in the nail polish remover is actually a solvent, and hot glue is not soluble in it. For using acetone to remove nail polish from your fingers, you use the brush to apply the nail polish remover right on top of the dried nail varnish. Your finger will then melt the nails completely after a while with each swipe of a finger just before they can dry up altogether. But for dissolving hot glue, try either carpenter's construction adhesive or polyurethane finish products- they're designed specifically to dissolve out other building materials.