When faced with the challenge of diagnosing SIBO, the breath test emerges as the most effective and patient-friendly option, as recommended by the American College of Gastroenterology (ACG) clinical guidelines. By measuring hydrogen, methane, and hydrogen sulfide levels in the breath after ingesting a sugar solution, this non-invasive test can detect bacterial overgrowth in the small intestine. While common 2-gas breath tests that measure only hydrogen and methane have their strengths, the advanced 3-gas breath test like Trio-Smart offers a more comprehensive analysis. Though invasive methods like small intestinal aspirate and culture are considered the gold standard, breath tests remain the most accessible and reliable choice for most patients seeking answers and relief from SIBO symptoms.