Usually, the physiological and pathological research of mice requires an environment of SPF level and harsh conditions. Usually three main categories should be focused on: facility environment, feed/water and plastics, husbandry operations and day-to-day management, and waste disposal. The facility environment needs to control temperature and humidity, air cleanliness, and reduce noise pollution. The cages are made of stainless steel; the feed requires balanced nutrients, vacuum and high-pressure sterilization, drinking water needs to be acidified to inhibit bacterial growth, and the bedding is hygroscopic and non-toxic. Smell. Daily feeding rules, and records of mouse strains, and breeding records. The final waste treatment includes the harmless treatment of sewage and solid waste, animal carcasses and sharps, and special infection and radioactive waste treatment.