The interplay between tubulin glycylation and glutamylation can be leveraged to regulate microtubule-based processes in cells by providing a mechanism for sequential deposition of these modifications on the axoneme. Glutamylation, which precedes glycylation during cilia biogenesis, enhances the recruitment of TTLL10 to microtubules. This sequential deposition ensures that TTLL10 is effectively recruited to microtubules that have been glutamylated first, allowing for the controlled addition of glycines by TTLL10. By modulating the levels of glutamylation and glycylation, cells can regulate the activity and localization of TTLL10, influencing the formation, stability, and function of cilia. This interplay between tubulin modifications provides a mechanism for fine-tuning the tubulin code and regulating microtubule dynamics in various cellular processes.