After extraction of nucleic acids from the sample on an automatic extractor (Mag DEA DNA / RNA 200 virus), one step qRT PCR is performed. Specific primers are used for highly conserved regions and double-labeled probes to enhance and differentiate RNA SARS-CoV-2 and other beta Coronaviridae such as MERS. The assay uses Taqmanprobe and detects parts of Ε (Envelope) and RdRp (RNA dependent RNA polymerase genes) and S (Spike protein) gene simultaneously. Internal Process Control (IPC), which is added during RNA extraction is detected in the same reaction as a labeled probe. Additionally, the assay includes the internal system control (ISC) which consists of primers and probes for the detection of a housekeeping gene, Beta-actin, during the extraction process. The ISC helps, preventing false-negative results due to insufficient sample drawing or inappropriate transport.