You can! However, you might need to wear shoes with extra room in the toes for a more comfortable workout. You should also make sure that you clean your toe separators more regularly, and keep in mind that your toe separators will wear out faster if you wear them while working out. You can sometimes wear a toe separator while working out, depending on your shoes and the design of your toe separator. You can wear triple-toe and double-toe separators, as well as some full-toe models composed of soft gel, while you’re working out.