Technically, yes. But you would only have 10 minutes or less at each shop and no time to stop for lunch! So we REALLY recommend doing the run in at least 2 days to ensure you have enough time at each shop. An example would be visiting the shops on the east end of the 401 one day and the west the other. Kingston is right in the middle and has 3 Participating Shops, so it’s a great place to rest for the night!