Yes, it is possible to take a screenshot of a video playing on your computer. However, the process may vary depending on the video player being used. Most video players have a built-in feature that allows users to capture screenshots while the video is playing. This can usually be accessed through the menu or by using keyboard shortcuts. In addition, there are also third-party software programs available that allow for more advanced screenshot features such as capturing multiple frames at once or automatically taking screenshots at designated intervals during playback.