MTCI module is used to communicate with different chamber controllers, troubleshooting procedures are below: If Monitor&Control Window shows MTCI channel reading 0.00 degree, it means Mitspro setting  issue: (1) Check ArbinSys.cfg, make sure MTCI is configured there, and the Auxiliary page has MTCI channel row’s Controllable checked. (2) Map/Batch file has MTCI channel correctly mapped to IV channel. (3) Iv channel already has a sdu assigned, and this sdu has added enough temperature channels inside. If Monitor&Control Window shoes MTCI channel reading 22 degree, it means Mitspro setting is ok, MTCI board or chamber controller issue: (1) Check MTCI board setting using ListenForNet, make sure MTCI’s controller type matches chamber’s controller type, and other parameters are matched. (2) Check the Chamber controller’s setting, and make sure the baudrate, parity, and other parameters match MTCI setting. (3) Check serial cable, some controller requires crossover wire, but some others do not. (4) Use Serial Port simulator/monitor software to communicate with MTCI or Chamber Controller directly.