The recommended method of using Olaplex 3 is to apply a generous amount of serum to the towel and then dried hair. After combing it, make sure you apply each strand, and usually leave it in hair for at least ten minutes before shampooing and conditioning your hair. If you have more hair care products with you, then you can shampoo your hair before applying the Olaplex also. It is recommend to keep it on hair for 10 minutes. But treatment is most effective after 30-45 minutes but it is effective as long as your hair is moisturized. Dozens of consumer reviewers shared that the treatment was put on the hair overnight, and the company recommends against it because you can give your eyes a serum and irritate them. Olaplex recommends using the treatment at least once a week to maintain healthy hair, but there is no risk of frequent use. At this level of use, most people can expect their 100ml Olaplex bottle at least 2 months.