This kit only detects Angiopoietin-2 and has shown no cross reactivity with Angiopoietin 1 or Tie 2 (soluble Tie 2 receptor). Our Angiopoietin-2 detection range is around 20-50 ng/mL, and therefore we consider your detection values were in the normal range. Although the written highest point is 10 ng/mL, this kit can detect even higher. We suggest you perform a dilution gradient to determine the appropriate detection range.