While the single-cell/single-nucleus approach offers valuable insights into the heterogeneity of skeletal muscle cell populations, there are potential limitations that need to be considered. One limitation is the inability to capture the spatial organization and interactions between different cell types within the muscle tissue. This approach provides a snapshot of the cellular landscape but may not fully represent the dynamic and three-dimensional nature of the skeletal muscle niche. Additionally, the dissociation process required for single-cell/nucleus sequencing may lead to cell stress and altered gene expression profiles, potentially skewing the results. To address these limitations in future studies, integrating spatial transcriptomics techniques with single-cell sequencing can provide a more comprehensive understanding of the cellular composition and organization within the skeletal muscle tissue. Furthermore, advancements in imaging technologies, such as multiplexed imaging and spatial proteomics, can help visualize the spatial relationships between different cell types and their microenvironment, enhancing our understanding of the skeletal muscle niche complexity beyond transcriptomic and epigenetic analyses.