There is no single test to check for a hormonal imbalance. If you are having symptoms that could be related to a hormone imbalance it is a good idea to make an appointment with a doctor to see if that is what you are dealing with. The doctor will start with a physical exam and ask questions about the symptoms that you are having. The doctor will also want to know all the medications, supplements, and vitamins that you are currently taking. Since hormones travel through the body in the bloodstream, a blood test will be taken to detect what your hormone levels are. The blood test is good at checking thyroid levels, estrogen and testosterone levels, and cortisol levels. A pelvic exam will allow the doctor to look for abnormalities, such as lumps, cysts, and tumors that can be symptoms of a hormone imbalance. An ultrasound may be requested to get images of the thyroid, pituitary gland, ovaries, uterus, and testicles to see if tumors or other abnormalities are present. In certain situations, more advanced tests may be needed. You may be required to get a biopsy, X-ray, MRI, thyroid scan, or sperm count test.