The mechanism used in next-generation high-throughput DNA sequencing from the options provided is:
- Ion Torrent
Explanation:
Ion Torrent: This technology is a type of next-generation sequencing that uses semiconductor-based sequencing to detect changes in pH as nucleotides are incorporated into the DNA strand. This method is known for its high-throughput capabilities and is distinct from traditional Sanger sequencing.
3 Step Method: This term is not a specific mechanism used in next-generation sequencing. It may refer to various procedural methods but is not a recognized technology or mechanism in high-throughput sequencing.
Chain Terminators: This is a mechanism used in Sanger sequencing, not in next-generation sequencing. Chain terminators are used to terminate DNA synthesis at specific points, which helps in determining the sequence of DNA fragments.
Sanger Sequencing: This is a traditional sequencing method that predates next-generation sequencing. It uses chain-terminating dideoxynucleotides to sequence DNA but is not considered high-throughput.