APF (Cat# 16050) is an ROS probe specific to hydroxyl radical (â—OH), peroxynitrite anion (ONOO−) and hypochlorite anion (ClO–).
HPF (Cat# 16051) is an ROS probe specific to â—OH and ONOO−.
DCFH-DA (Cat# 15204) is an ROS probe responsive to all ROS species and is often used as a total ROS probe.
APF and HPF are more stable and have low auto-oxidation effect compared to DCFH-DA, which is easily oxidized. We have observed a gradual increase in fluorescence over time in DCFH-DA stained cells that are exposed to excitation light under microscope.