AFC substrates absorb and emit at higher wavelengths:
released 7-amino-4-trifluoromethylcoumarin λexc 395-400 nm, λem 495-505 nm
released 7-amino-4-methylcoumarin λexc 360-380 nm, λem 440-460 nm
Please see also our Technical Note “Chromophors/Fluorophors: Spectral Properties and Characteristics” which can be downloaded from our homepage.