Glycans contribute significantly to the heterogeneity of the protein’s mass and charge and can also impact protein activity. During development glycan profiling can be used to (i) identify the best clone, (ii) the best media and/or (iii) the best process. Additionally, during clinical manufacturing and market supply glycan profiling can be used to monitor product quality. In later stage clinical trials and market supply glycan profiling is often used as release test.