Yes. Essentially, prepaid debit cards are different from debit cards attached to bank accounts. You won't have any problems playing with a prepaid debit card if you are the only one using the card.
However, if you plan on playing at a casino that allows your prepaid card and someone else is using your card, make sure you check with the casino's terms of service to find out if you can create another account. Debit card casinos online will rarely accept multiple registrations from the same computer/IP/ household as a way of curbing abuse of welcome bonuses.