Your doctor will prescribe blood tests to check your levels of serum methotrexate concentration (in case of methotrexate chemotherapy or overdose) to determine the optimal dose and duration of treatment with Shilstine 50mg Injection. You will also be prescribed a complete blood count to monitor the number of red blood cells (in case of megaloblastic anemia). Along with this, your calcium levels will also be monitored in order to prevent hypercalcemia (excess calcium) since Shilstine 50mg Injection contains calcium.