Yes, anime characters often use different words for 'yes' and 'no' depending on their level of formality or casualness. For example, in more formal situations, characters may use 'hai' for 'yes' and 'iie' for 'no.' In less formal situations, they may use 'ee' or 'un' for 'yes' and 'iie' or 'chigau' for 'no.'