In short, yes. When you wear your dentures for long periods of time, the gums underneath do not get enough oxygen to “breathe”, and the moisture in your mouth can lead to bacterial and even fungal growth. That’s why it’s best practice to take them out every night and clean them thoroughly. For some patients with atypical schedules, it makes sense to remove them during the day instead. The main idea is to give your gums time to rest and “breathe” at some point every day. Also, for people who grind their teeth, taking their dentures out while sleeping will increase their usable life.