For the Rat VEGF ELISA Kit PicoKine (EK0540), there is no wash step between step number 5 (removal of sample following 120 min incubation) and step number 6 (addition of biotinylated Anti-Rat VEGF).
Our lab technicians found there wasn't any significant difference in result values between washing and without washing the plate after incubating samples/standard and before addition of the detection antibody. It is to make the protocol easy and fast.