Yes, it is safe to use cooking spray on a teflon pan. In fact, it can help to extend the life of your teflon pan by providing a barrier against scratches and other damage. However, it's important to use a light hand when applying cooking spray to a teflon pan, as too much can cause the pan to become sticky and difficult to clean. If you do accidentally use too much cooking spray, you can try wiping the excess away with a paper towel or damp cloth.