The main difference between a fresh and frozen embryo transfer is the timeline of insemination and development. For instance, with fresh embryos, the transfer occurs on day 3 or 5 of the female recipient's cycle, followed by a blood test pregnancy confirmation 2 weeks after the transfer occurs. With frozen embryos, more steps are taken to confirm ovulation and uterine health before insemination. Typically, frozen embryo transfers happen on the 6th day after a series of progesterone injections, followed by additional weeks of progesterone and estradiol doses until pregnancy test results can be seen.