The loss of alpha-helix or beta-sheet structure and the formation of intermolecular beta-sheet is associated with stability loss. FT-IR and CD are the most prevalent techniques to detect these secondary structures. However, FT-IR suffers from low sensitivity and reproducibility to detect small changes in the secondary structure. CD on the other hand is limited by a low concentration range and less sensitive towards the prediction of beta-sheet compared to alpha-helix structures.
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