High levels of bilirubin could mean your liver isn't functioning properly. However, high levels may also be due to medications, exercise, or fast foods. Bilirubin is additionally a product of breakdown of red blood cells, an elevated reading is also associated with disorders of red blood cells and not liver disease.An orthodox diazo technique is popularly used for measurement of bilirubin. On the other hand, an enzymatic technique that employs bilirubin oxidase, has additionally been in use for considerable time. Bilirubin oxidises slowly once exposed to light, therefore specimens obtained for bilirubin levels ought to be shielded from light exposure.