After many experiments, I have come to the conclusion that if you want it to be juicy, you need to bake it for 50 minutes at 350 degrees F. I have tried different temperatures and cooking times (such as 40 minutes at 400 degrees), but ultimately, we were all happiest with 50 minutes at 350 degrees. Of course, ovens greatly vary in their temperature. So the best way to make sure that the meatloaf is done is to use an instant-read thermometer. The internal temperature should reach 160° F.