Wrinkle length and wrinkle depth can be measured with a Visioline VL 650 measuring instrument. The measuring principle is based on the skin's relief, and thus wrinkles, being replicated using a material called Silflo. The wrinkles, which normally go down into the skin, will be raised on the replica like a microscopic "mountain" rising up. These "mountains" are illuminated at a special angle so that shadows are formed. The shadows are measurable and visualized using a camera. The measured shadows are used as a basis in mathematical models, so that quantitative measures of wrinkle parameters such as depth and length of wrinkles are obtained.
Method: Test subjects are instructed to stop using other face creams/serums and the like when the test period starts. The test subjects apply the test product to their face twice a day, as the only skin care product, for 8 weeks. Wrinkle parameters are measured on day 0, and then after 8 weeks. Measurement of the skin's wrinkles takes place at both times in a room with a temperature of 20±2oC and a humidity of 50±10%, so that the result is not affected by the environment. The results of the measurements before and after 8 weeks of using the test product are then compared. In the clinical study, the effect of the test product is compared in this way with the skin care the test person has used in the past.
The test is in accordance with:
Regulation of the European Parliament and of the Council (EC) No. 1223/2009 of 30
November 2009 on cosmetic products.
Cosmetics Europe – The Personal Care Association (previously COLIPA) Guidelines
"Product Test Guidelines for the Assessment of Human Skin Compatibility 1997." Cosmetics Europe – The Personal Care Association (previously COLIPA) Guidelines for the Evaluation of the Efficacy of Cosmetic Products 2008.