Please ensure that the buffer used for CELLstart has Calcium and Magnesium. Please review the product insert for our recommended buffer. If the right buffer is used, increase the concentration of CELLstart up to 1:50 dilution rate. If CELLstart with our recommended buffer does not work, some users had success with Poly-L-Ornithine–coated plates with StemPro NSC-SFM. If you use Poly-L-Orinithine, please perform overnight incubation.