The applicable procedures of ultra−high-performance liquid chromatography (UHPLC) are the analysis of assay and organic impurities, related substances, drug products, or raw material of pharmaceuticals or degradation products of a drug substance or intermediate. A suitable method is developed only after assessing chromatography’s main and critical separation parameters (examples for UPLC-HPLC are wavelength, a choice of diluent, stationary phase, detector, flow rate, column temperature, solvent system, injection volume, and elution mode, Etc.). The development of the analytical method shows that the developed analytical approach is appropriate for its proposed use for the quantitative assessment of the targeted analyte present in therapeutic drugs. And it typically plays a vital part in the development and making of pharmaceutical medications.