Cells cultured in standard flat (2D) culturing flasks or wells tend to adapt to this unfamiliar environment by stretching out randomly along the surface and thus becoming more or less flat. This causes the cells to effectively lose roughly 50 % of their surface area in contact with surrounding culture medium as well as reducing possible cell-to-cell interactions by the same amount. By creating a network of electrospun nanofibers for the cells to grow on, we provide the cells with a three dimensional (3D) environment, similar to collagen and elastin fibers of the extracellular matrix (ECM) that cells normally reside in. Adding a third dimension to a cells environment creates significant differences in cellular characteristics and behavior such as differentiation, drug metabolism, gene expression and proliferation. Importantly, these differences normally create a greater similarity between the cultured cells and the living organism (e.g. human being) the cells are meant to represent and leading to more useful data and more relevant research. For more information and a collection of relevant publications please have a look at www.3DCellculture.com.