No, Lou Bloom never blinks throughout the entirety of the movie Nightcrawler. This fact is highlighted in multiple scenes and serves to emphasize his robotic-like nature. His lack of blinking could represent his inhumanity and detachment from reality; he has no need for simple human functions like blinking. It could also be interpreted as a sign of fearlessness or confidence; he refuses to blink even while facing danger because he believes himself to be untouchable. Whatever the intended message, it's clear that Lou Bloom's unwavering stare is one of the most iconic elements of this cult classic film.