The isolate of seed-borne virus was separated from Petunia hybrida seedlings showing symptoms of vein necrosis. Electron microscopy revealed bacilliform particles of four main sizes ranging from 30 to 60 nm. Test-plants’ local and systemic reactions were characteristic of Alfalfa mosaic alfamovirus (AMV). AMV identification was confirmed by a positive reaction in DAS-ELISA assay and obtaining specific for this virus amplification products in RT-PCR.
Keywords: Petunia, Alfalfa mosaic virus, identification, DAS-ELISA, RT-PCR.