Cupcakes should be eaten the day they are baked as I use only natural, high end ingredients, no preservatives. If you would like to eat them the following day, I suggest you place the cupcakes in an airtight container or place the box in a sealed plastic bag and refrigerate. For serving remove cupcakes from the refrigerator 3-4 hours in advance and leave at room temperature. Cupcakes with cream cheese, mascarpone, or whipped cream do need to be refrigerated.