Here’s how this process typically works:
Cell expansion: Stem cells are initially obtained from a donor or patient. These cells are then cultured and expanded in a controlled environment. This involves providing the cells with appropriate growth factors, nutrients, and conditions to encourage them to multiply.
Cell viability: Throughout the expansion process, the viability of the cells is monitored. Viability assessments determine the percentage of live, functional cells in the culture. Techniques like trypan blue exclusion or flow cytometry can help determine cell viability.
Cell counting: Regular cell counting is performed to track the number of cells as they multiply. Various methods, such as automated cell counters or manual hemocytometers, can be used to count cells accurately.
Proliferation rate: Monitoring the proliferation rate helps determine how quickly the cells are dividing. Adjusting growth conditions can control the rate of expansion to achieve the desired cell quantity.
Quality control: The cells are assessed for quality, which includes characteristics like surface markers, genetic stability, and differentiation potential. Quality control ensures that the cells maintain their therapeutic potential.
Safety control: Numerous tests are performed to check cells for sterility and possible infections, including HIV, hepatitis B, C, herpes, CMV, toxoplasma, mycoplasma, and endotoxins.
Storage and injection: Once tested, the cells are cryopreserved in liquid nitrogen until they are needed. Cells are then administered into the patient’s body in the sterile conditions of the clinic. Since we have our own laboratory in the clinic, there are no concerns regarding transportation.