Please suggest the customer follow the two tips listed below to improve the assay.
1. Use diluiton of 1:1 or undiluted samples
2. The cell concentation is recommended to be 106/ml.
If the customer had run the assay with the above mentioned samples and obtained blank data, which indicated that the expression level of Gal-4 in primary Chondrocytes was too low to be detected.
Gal-4 concnetration in serum and plasma is very low and we are suspecting that the primary Chondrocytes doesn't contain too much Gal-4.