Vo filme Kráľovná Alžbeta: Zlatý vek hrajú Cate Blanchett (Elizabeth I, Queen of England), Clive Owen (Sir Walter Raleigh), Geoffrey Rush (Sir Francis Walsingham), Laurence Fox (Sir Christopher Hatton), Tom Hollander (Amyas Paulet), Abbie Cornish (Elizabeth Throckmorton), Rhys Ifans (Robert Reston), Jordi Mollà (King Philip II of Spain), Samantha Morton (Mary, Queen of Scots), Eddie Redmayne (Anthony Babington), Adrian Scarborough (Calley), Adam Godley (William Walsingham), Christian Brassington (Archduke Charles), Robert Cambrinus (Count Georg von Helfenstein), David Threlfall (Dr. John Dee), Vidal Sancho (Spanish Minister), Kelly Hunter (Ursula Walsingham), John Shrapnel (Lord Howard), Sam Spruell (Torturer), David Sterne (Cellarman), David Robb (Admiral Sir William Winter), Jonathan Bailey (Courtier), Steve Lately (Walsingham's Servant), Kate Fleetwood (Woman with Baby), Aimee King (Infanta Isabel of Spain), Susan Lynch (Annette), Kristin Coulter Smith (Mary Walsingham), Hayley Burroughs (Queen Elizabeth's Waiting Lady #1), Kirsty McKay (Queen Elizabeth's Waiting Lady #2), Lucia Ruck Keene (Queen Elizabeth's Waiting Lady #3), Lucienne Venisse-Back (Queen Elizabeth's Waiting Lady #4), Elise McCave (Laundry Woman), Penelope McGhie (Margaret), Coral Beed (First Court Lady), Rosalind Halstead (Second Court Lady), Steven Loton (Manteo), Martin Baron (Wanchese), David Armand (Walsingham's Agent), Steven Robertson (Sir Francis Throckmorton), Jeremy Barker (Ramsey), George Innes (Burton), Kirstin Smith (Mary Walsingham), Tim Preece (Old Throckmorton), Benjamin May (Dance Master), Glenn Doherty (Royal Servant), Chris Brailsford (Dean of Peterborough), Dave Legeno (Executioner), Antony Carrick (Spanish Archbishop), John Atterbury (Marriage Priest), Alex Giannini (First Spanish Officer), Joe Ferrara (Second Spanish Officer), Alexander Barnes (Courtier), Charles Bruce (Courtier), Jeremy Cracknell (Courtier), Benedict Green (Courtier), Adam Smith (Courtier), Simon Stratton (Courtier), Crispin Swayne (Courtier), Kitty Fox (Mary Stuart's Lady in Waiting), Kate Lindesay (Mary Stuart's Lady in Waiting), Katherine Templar (Mary Stuart's Lady in Waiting), Morne Botes (Courtier (uncredited)), Finn Morrell (Young Boy (uncredited)), Shane Nolan (Tyger Salior (uncredited)).