That’s a great question! Successful transfection is influenced by many factors—the choice of the transfection method, viability of the cells prior to transfection, number of passages, degree of confluency, quality and quantity of the nucleic acid used, the amount of reagent can all play a part in the outcome of your transfection experiments. YYou can visit our Transfection page and go over the section ‘Find the Right Transfection Reagent’ to get more detailed information on cell lines that you work with.