Passage Reagent Group is a matched set of Cell Systems Certified Reagents for releasing cells from culture for subculture or freezing. The PRG contains three parts:PRG-1(EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The chelating agent EDTA in PRG-1 prepares for PRG-2, which contains highly purified trypsin. PRG-3 inactivates the protease in PRG-2 and stabilizes the cell membranes.