Scaling up the photobiocatalytic technology for industrial-scale production of stereoselective non-canonical amino acids may face several limitations and challenges. One major challenge is the stability and activity of the engineered pyridoxal biocatalysts under large-scale reaction conditions. Ensuring the reproducibility and robustness of the enzymatic system at a commercial scale is crucial for maintaining high yields and selectivity. Another limitation could be the cost and availability of the required photocatalysts and oxidizing agents, which may impact the economic feasibility of the process. Furthermore, optimizing the reaction parameters, such as light intensity, temperature, and reaction time, for large-scale production while maintaining stereoselectivity can be a complex task that requires extensive process development and optimization.