There are known protocols for isolating MSCs from different tissues. The protocols are well-published and the cells can usually be harvested in aesthetic clinics (e.g.,   during liposuction) and orthopedic wards. The stages include lysis of cells (mainly fibroblast), connective tissue digestion, pull down of MSCs, and seeding.
NutriStem MSC XF Basal Medium is suitable to use from the earliest stage. The medium should be used after the lysis step and before seeding to wash the cells. The medium should be equilibrated at the incubator. The complete medium is used in the later stages of isolation, and in the proliferation and seeding of the MSCs. You can obtain MSCs from easily accessible sources using minimally invasive methods, and can rapidly expand them in large-scale clinical use for patient-specific, autologous therapies.