Proximal tubule cells are plated on 24 well Transwell plates
Parallel assays are available using PTCs from a range of species: Human, Dog, Rat, Mouse and Non-Human Primate
Cell monolayers undergo QC (TEER of >60 ohms.cm2)
We measure Absorptive flux (apical to basolateral; Jab), Secretory Flux (basolateral to apical; Jba) and calculate Net flux (Jnet= Jba-Jab)
We can measure transport of either small molecules or large molecules in our system
We can measure radiolabelled compounds using scintillation counting or non- labelled compounds by LC-MS
We correct for paracellular flux using either radiolabelled Mannitol or unlabelled Lucifer Yellow
We include a positive control (usually 3H-PAH or 14C-creatinine) as a QC of assay performance
To understand mechanisms of transport or Drug-Drug interactions, we can run experiments in the presence of specific inhibitors of transport proteins.
We provide either the raw data or a full package of transcribed and analysed data, data summary and a report