Yes, to begin with, the vehicle must be USA OBD2 compliant.
To start a test, the ignition must be in the ON position with the engine in the OFF position (KOEO).  Depending on which test is selected, the user will be prompted to start the vehicle to continue running tests. To ensure the vehicle is in the proper state, the following vehicle pre-conditions must be met before the test will continue:
Engine RPM
Coolant temperature
Loop Status