No. The neurologic examination itself is not painful. Some dogs/cats don’t like the part of the examination where their paws need to be touched but this is usually short lived and generally doesn’t cause a lot of stress. Imaging tests like MRI and CT scan are not invasive and are performed under general anesthesia. Spinal tap is also performed under anesthesia and electrodiagnostics are performed under heavy sedation or anesthesia and so there is no discomfort involved in these procedures.