Ans. The length of the nuclease's recognition sequence and the complexity of the DNA determine the frequency at which a particular nuclease will cut a given DNA molecule. A longer recognition sequence results in less frequent digestion because of the statistical chance of finding the bases in a particular order randomly. For instance, the occurrence prediction for a given four-base sequence (that corresponds to the recognition site for a hypothetical nuclease) is going to be every 256 base pairs on average (where 44 = 256), but for any given six-base sequence it would be once every 4,096 base pairs on average (46 = 4096)Meganuclease is a unique family of nucleases characterized by having larger, and less common, recognition sequences that consist of 12 to 40 base pairs. These nucleases are especially useful for Genome engineering and genetic engineering applications in complex organisms such as mammals and plants. These are cases where typically larger genomes (billions of base pairs) would result in frequent site-specific digestion with the help of traditional nucleases.