Vo filme Králiček Jojo hrajú Roman Griffin Davis (Jojo), Thomasin McKenzie (Elsa), Scarlett Johansson (Rosie), Taika Waititi (Adolf), Sam Rockwell (Captain Klenzendorf), Rebel Wilson (Fraulein Rahm), Alfie Allen (Finkel), Stephen Merchant (Deertz), Archie Yates (Yorki), Luke Brandon Field (Christoph), Sam Haygarth (Hans), Stanislav Callas (Russian Soldier), Joe Weintraub (Herr Junker), Brian Caspe (Herr Mueller), Gabriel Andrews (Herr Klum), Billy Rayner (Herr Frosch), Christian Howlings (Bazooka Boy), Gilby Griffin Davis (Clone), Hardy Griffin Davis (Clone), Curtis Matthew (Doctor), Robert East (Herr Grusch), James McVan (American Soldier (uncredited)), Judith Georgi (German Translator (uncredited)), Victoria Hogan (Woman (uncredited)), Bethany Adams (Secretary (uncredited)), Iva Šindelková (Postwoman (uncredited)), Matej Seifert (Soldier (uncredited)), Trish Osmond (Old Woman (uncredited)), Odeta Cali (Gestapo Secretary (uncredited)), Samuel Bogner (Hitlerjugend (uncredited)), Issy Stewart (Kissing Girl (uncredited)).