The amount of sealant required depends on the width of the tire. The sealant should coat the inside of the tire walls and there should be a small puddle in the bottom. Use these values as a base, but more sealant would always give more protection. 25mm tires – 40-50ml 28mm tires – 45-55ml 32mm tires – 55-65ml 40mm tires – 70-90ml 45mm tires – 80-100ml 2.1” tires – 100-120ml 2.25”+ tires – 120+ml The sealant is water-based and dries out over time. Do not leave the tires in the direct sunlight as this accelerates the drying. Refill the sealant every 3-4 months.