We recommend 50-100 microspheres per analyte in a Luminex multiplex bead assay to obtain highly accurate and precise results. To meet your unique scientific objectives, we sometimes increase the number of events even as most commercial Luminex multiplex assay protocols recommend 50 bead events per analyte per sample. The increased bead count does not affect the kinetics of the multiplex immunoassay. It only increases the time needed to acquire results per sample. Generally, each Luminex assay takes about 30 mins to 1 hour to analyze a 96 well plate on the platform. That said, we would sometimes need to spend several hours on meticulous sample preparation and incubation.