In order to keep sterility and workers’ safety during the IMMUNOBIOGRAM process, it is necessary to work within a biological safety cabinet, to sterilize all the materials used, to filter all the reagents, and to ensure a proper hygiene and training of the lab technicians. In case of a cultured PBMCs’ contamination, the sample should be discarded, regardless of the step of the process when the contamination appears. It is crucial to discard this kind of samples to avoid a potential influence in the IMMUNOBIOGRAM results.