While cryo-EM has provided valuable structural insights into the human NET, there are certain limitations and caveats associated with this technique. One limitation is the potential for conformational heterogeneity, where different states of the protein may exist but only one is captured in the structure. Additionally, cryo-EM structures may not fully capture the dynamics of the protein in its native environment. Future studies could address these limitations by employing complementary techniques such as molecular dynamics simulations, X-ray crystallography, or functional assays to validate the observed structures and provide a more comprehensive understanding of NET dynamics and function.