During the process of differential centrifugation, larger, denser components of a biological sample sediment to the bottom of the tube, while smaller, less dense components remain suspended in the supernatant. The specific components that remain in the supernatant will depend on the specific sample being processed and the conditions used for the centrifugation. In general, the supernatant of differential centrifugation may contain a variety of components, including cells, organelles, proteins, and other small molecules. The components that remain in the supernatant may be further separated and purified using additional techniques, such as density gradient centrifugation, chromatography, or immunoprecipitation. Overall, the supernatant of differential centrifugation contains a mixture of smaller, less dense components that have not sedimented during the centrifugation process. These components may be of interest for further study or analysis, and can be further purified using additional techniques as needed.