The insemination must be done at the right time. It should either be done during ovulation or right before ovulation. Usually, the sperm can stay alive in the female reproductive tract for up to 5 days (after having intercourse), because of which they can fertilize an egg. Although, the egg can be fertilized only within 12–24 hours post-release. IUI insemination must be at the right time so that the egg and the sperm are present in the fallopian tube at the same time. That’s why the process of IUI is mostly done one or two days after ovulation. Your fertility expert will align the insemination date with your ovulation dates.