Live cell imaging is a specific tool for cellular research with wide variety of applications. By streaming digital microscopic images, an observer can observe dynamic morphology of cell, can track cell movement on the surface and can measures fluorescently labeled proteins or molecules. These single cell imaging systems are designed to operate the inside cell culture incubator and enables to maintain the cells under optimum conditions. It is a vital tool for getting maximum information from precious cell samples. Also, it allows observations of cell interaction, behavior of the cell. By using live cell imaging it is possible to study how molecules translocate, interact and respond to environmental cues. Also some imaging systems are able to monitor the molecules within live animals. Live-cell imaging offers spatial & temporal information of dynamic events in single cells, cellular networks (in situ) or even whole organisms (in vivo). There are some microscopic methods applied for live cell imaging such as time lapse imaging, advanced fluorescence techniques such as ion imaging, FRET (Froster Resonance Energy Transfer), TIRF (Total Internal Reflection) microscopy, MPE (Multiphoton Excitation).