Yes. In addition to moderate light and clean water, an Anubias nana plant will require CO² to survive. In most cases, this plant can live on the CO² that your aquarium’s fish naturally expel. If your habitat does not have a lot of fish, the water may not have enough CO² for your Anubias to thrive. You can correct this by adding supplements to the water—just pay careful attention to the balance of chemicals in your habitat, so it doesn’t adversely affect other plant or aquatic life.