Sperm preparation is a lab technique used during IUI to separate motile sperm from non-motile sperm and other components, such as seminal fluid, of human semen.
In the case of near-normal specimens, ejaculate is mixed with a washing medium after liquefying and centrifuging.
After discarding the supernatant, it is necessary to resuspend the sediment in a washing medium. This process is repeated 2-3 times maximum. In the final wash, the residue is resuspended in 0.5 ccs of the medium and then loaded into a syringe and then deposited into the uterus.