This depends on the kit chemistry and sample type. Some NGS kits are robust at a wide range of volumes and sample concentrations, while others allow up to a 4-fold volume reduction. Sample type (e.g. single cells vs bulk DNA/RNA, or high vs low RNA content) should also be considered. Generally single cell workflows allow substantial miniaturization as the input is just a few picograms of genetic material. In the case of bulk RNA and DNA miniaturization, it is still possible but should be less extreme to ensure sufficient coverage and depth of sequence data. Samples for bacterial and viral genomic amplicons, due to their low complexity, can be processed using low volumes of the reagents.