Peptides must be warmed to Romm temperature before being opened since they are hygroscopic. The most effective technique to prevent water condensation from accumulating on the peptide is to warm in a desiccator or dry box. During peptide manufacturing, it is crucial to minimize contamination. This can be achieved by weighing out the product in a sterile atmosphere, ensuring only the required amount is used. Afterward, the containers must be resealed carefully. Any unused peptide should be stored in a sealed container with a desiccant, preferably at a temperature of -20°C or lower. By adhering to these storage conditions, bacterial breakdown, the formation of secondary structures, and oxidation can be significantly reduced or even halted for several years. Cysteine, methionine, tryptophan, asparagine, glutamine, and N-terminal glutamic acid-containing sequences will degrade more quickly than other peptides. If your experimental setup permits it, please utilize the suggested solvents listed in the solubility test that CPC provides for solubilizing the peptide. The GMP peptide can often be broken down by sonication. In general, sterile water is the best solvent, although depending on the peptide's amino acid sequence, acidic peptides will dissolve in a basic buffer and basic peptides in an acidic buffer. Since they are particularly prone to oxidation, Cys, Met, and Trp-containing peptide sequences demand oxygen-free solvents. The stability and shelf life of peptides in solution are significantly lower than those in lyophilized form. In order to increase shelf life, peptides in solution should be stored at -20 C using sterile buffers with a pH of 5–6 rather than under these conditions, which is not advised. Having CPC aliquot the peptide is the best method to steer clear of this problem.