Yes. The Corning Elplasia plates feature a clear bottom and low background autofluorescence. We have successfully imaged with the Elplasia plates using a 20x objective with a confocal imager. Corning Elplasia are also compatible with staining protocols. However, you should take care when adding reagents to the well in order to not disturb the spheroids. We recommend avoiding pipetting near the bottom of the wells as well.