Type II topoisomerases require the energy of ATP hydrolysis for their function. This is because the mechanism of action of Type II topoisomerases involves breaking both strands of DNA simultaneously, passing another DNA duplex through the gap, and then resealing the DNA strands. These processes require a significant amount of energy to overcome the resistance of the DNA double helix and facilitate the strand passage. ATP hydrolysis provides the necessary energy to drive the conformational changes in Type II topoisomerases. The ATP molecule is hydrolyzed to ADP and inorganic phosphate (Pi), releasing energy that can be utilized by the enzyme to perform its functions. The energy from ATP hydrolysis is used to induce the conformational changes required for DNA cleavage, strand passage, and DNA resealing. In addition to providing energy, ATP hydrolysis also regulates the activity of Type II topoisomerases. The binding and hydrolysis of ATP help coordinate the enzymatic cycle, ensuring the proper timing and sequence of events during DNA manipulation. In summary, the energy from ATP hydrolysis is necessary for the function of Type II topoisomerases as it enables the breaking and resealing of DNA strands and facilitates the passage of DNA through the enzyme.