Isolation of iPSCs from a master reprogramming plate must be done manually. Colonies should be selected through the use of a 27-gauge needle and a pipettor and then transferred to individual vessels for clonal expansion. Once the PSCs have been expanded they may then be split through future passages by the same manual selection (using a needle) or bulk passaged using a tool like the StemPro EZPassage Disposable Stem Cell Passaging Tool.
Another option for non-enzymatic expansion of PSCs is to use UltraPure EDTA to dissociate the cells from the plate. This is only applicable when cells are cultured in a feeder-free media like Essential 8 medium on either vitronectin or Geltrex coated TC dishes.
Learn more about Essential 8 medium