You want twice as much high glucose DMEM as usual. To create a solution from a liquid glucose concentration, combine 44.8 ml of 1 M glucose and 955.2 ml of 5.5 mM DMEM. - Alternatively, just mix 8 g of D-glucose into 1 L of low-glucose DMEM. The D-glucose will immediately begin to dissolve. The mixture should be sterile filtered before use (0.22 μm pore size). Blood sugar levels over 400 mg/dl will cause brain damage. Therefore, you need to reduce these levels quickly! The only treatment for diabetes is insulin, which controls glucose levels by regulating its uptake by body cells. However, even with insulin therapy, people with diabetes are at risk for developing serious complications such as heart disease, stroke, blindness, amputations, and kidney failure. People with type 2 diabetes cannot properly produce or respond to insulin, so they must take injections or pills to control their glucose levels. The two main types of medications used to treat diabetes are insulin and oral drugs. There are four main classes of oral drugs: alpha-glycosidics, biguanides, thiazolidinediones, and glitazones. Alpha-glycosidics drugs include glyburide (Glibenclamide) and nateglutide (NebuPentio).