Current methods to track the efficiency and success of stem cell differentiation require the allotment of a specific well or wells of cells that are stained for stage-specific markers along the differentiation process. The non-invasive methods utilized by Proteome Profiler Arrays and Luminex Assays enable researchers to be able to monitor the same stem cell population throughout the differentiation protocol, while preserving the ability to use those same cells for downstream application once they have been fully differentiated. In addition, using a pre-identified secretory profile for successful differentiation, researchers may be able push cells that aren’t differentiating efficiently into a more successful trajectory by manipulating cell culture media so that it reflects the conditions observed in successfully differentiating cells.