For Oris assays, add test compounds to the culture medium once the cells have attached and the stoppers have been removed, but before migration begins. For Oris Pro Assays, add test compounds once the BCG has dissolved and the cells have attached, but before migration begins. For both Oris and Oris Pro assays, adhesion time is dependent on cell line and plate coating, with attachment times. You may remove and replace the medium prior to adding test compounds in order to eliminate any non-adherent cells. In the invasion assays, compounds may be incorporated into the collagen I solutions and/or added to the culture medium above the collagen.