As per the minimal criteria defined by ISCT in 2006, in addition to in vitro differentiation of multipotent mesenchymal stromal cells (MSC) into adipocytes, osteoblasts and chondroblasts, cells must adhere to plastic and show positive expression of CD105, CD73, CD90 and absence of CD45, CD34, CD14, CD19 and HLA-DR {Dominici et al., Cytotherapy (2006) 8, 315-317}. Phenotypic characterization can be carried out via flow cytometric analysis of antibody stained cells.