While liquid chromatography (LC) coupled with mass spectrometry (MS) (LC-MS) is a powerful and commonly used technique for the characterization of biopharmaceuticals during drug development, LC coupled with ultraviolet-visible (UV-Vis)/photodiode array (PDA), fluorescence and light-scattering detectors is used for routine analysis. A variety of LC methods have been developed to assess the critical quality attributes of these molecules, such as purity and chemical modifications to biotherapeutic molecules, aggregation and charge variants, as well as to quantify them in complex matrices and ensure the absence of impurities.