Before you begin sample collection, make sure you have checked your toilet for any cleaners, bluing agents, deodorizers, and rust, then proceed. Flush your toilet twice and make your bowel movement in clean water. Toilet paper cannot go into the toilet bowl. Be sure to place any used toilet paper in one of the blue waste bags located in the BRUSH KIT. Take one of the blue brushes from your BRUSH kit and gently brush stool for 5 seconds to collect your water sample. Tap the brush once on the inner rim of your toilet seat to remove any excess water, as seen in the photo on your printed collection instructions or as seen in our online video. Lift the blue flap on your test card to reveal two white test squares. Dab the surface of square 1 with the brush water for about 5 seconds. Repeat that same process with the second brush to apply water sample to square 2. You can use the second blue waste bag to discard both brushes and then discard into your household trash. Make sure to seal your test card flap down with the label that you wrote your name, date of birth, and collection date on.