Biophysics is a demanding course and requires a key understanding of the concepts and theories. The subjects require a quantitative and mechanical understanding of experiments and methodologies. The course is challenging since the experimentation results and procedure may vary from one person to another. Therefore, the study does not entirely depend on the bookish concepts and theories stated. Physics and mathematics themselves are tough subjects, and their application in the study of biophysics helps in achieving better results and knowledge. Since, the course is a cluster of various disciplines, therefore the learning and understanding complexity increases.