The typical operational steps include performing a system integrity test before operation, deaeration to remove air, and flushing with Water for Injection (WFI) for cleanliness. Additional steps include pre-use visual leakage testing, calibration of flowmeters and pressure transmitters, and product filtration. After filtration, the system undergoes a rinse with Water for Injection, a filter wash with EQ buffer, and post-use visual leakage test.