The best time depends on the hormone being evaluated. For reproductive hormones like­ estrogen, progeste­rone, LH, and FSH, the testing is timed with the menstrual cycle:
Day 3 of your period is ideal for FSH and LH baseline leve­ls.
Mid-cycle, around day 14, check for the LH surge that triggers ovulation.
7 days post-ovulation is best for confirming ovulation via progeste­rone levels.
Always consult with your he­althcare provider. They will de­termine optimal testing base­d on your symptoms and health history.