Ans. Yes, you can. That’s because it neither holds onto nutrients nor decomposes. In order to use perlite again, all you have to do is remove plant bits from it if you find any - for example, root segments. Once you do that, rinse the perlite thoroughly with water. If you want, you can sterilize it. For that, you’ll have to use a 10% bleach solution. Dilute the solution with water and soak the perlite in it for about 20 minutes. Give it a good rinse again, and you’re all set to reuse it!