According to the MTO, e-bikes can be driven on most roads where bicycles are allowed. However, you cannot ride an e-bike on:
Certain provincial highways, including the 400 highways, Queen Elizabeth Way, Queensway, or Kitchener-Waterloo expressway;
Municipal roads that have banned bicycles under bylaws; and
Certain municipal roads, sidewalks, and bike paths, trails or lanes, which have prohibited the operation of e-bikes.
While operating your e-bike you must follow the same rules that apply to cyclists.