Like a standard PCR machine, a qPCR machine consists of a heated block and lid that facilitates the rapid transition of samples between temperatures to enable amplification of a DNA or cDNA template. However, a qPCR machine also incorporates a fluorescent source and fluorometer to excite the fluorophores and detect the fluorescent output generated during cycles of qPCR amplification. The machine typically has or is linked to a computer that records the fluorimeter output and uses software to interpret the experimental results based on user-defined information such as control wells and standards.