No. Federal agencies no longer need to screen employees based on their vaccination status, and the federal government does not treat employees differently based on whether they are up-to-date with their vaccinations. In addition, employees, contractors and visitors aren't asked for proof of vaccination before entering federal buildings. Some facilities in settings that require it will continue testing all employees. See the Safer Federal Workforce website for more information.
In addition, the Equal Employment Opportunity Commission requires employers to assess pandemic and workplace circumstances in order to justify mandatory COVID-19 testing of employees. See the Equal Employment Opportunity website for more information.