Natural England states that you should use “…one kit per pond up to an area of 1 hectare. Beyond this, use an additional kit per hectare”. For example, two kits should be used for a pond between 1-2 hectares in size, and each kit should be used to sample one half of the pond in accordance with the Natural England protocol (i.e. one kit used to collect 20 subsamples from one half of the pond, and the other kit used to collect 20 subsamples from the other half of the pond).