File powder has a unique flavor profile with earthy and slightly tangy notes. If you're looking for a substitute, a combination of equal parts thyme, basil, and oregano, or ground sassafras leaves can provide a similar taste to file powder in dishes like gumbo or other Cajun and Creole recipes. However, keep in mind that file powder has a specific flavor, and while these substitutes can come close, they won't be an exact match.