To prepare 20 L of 1X Sheath Fluid, pour entire contents into a 20 L Cubitainer and add dH20 to a final 20 L volume. For cytometers with smaller sheath tanks, add 100 mL of 30X Sheath Fluid to 2900 mL of dH 2 0 to make up 3 L of 1X Sheath Fluid.