DIY kit vs. CST custom conjugation: Comparison of PE conjugation of Helios (D8W4X) XP Rabbit mAb by flow cytometry using CST custom conjugation method (blue) or another company’s DIY conjugation kit (red), on both RL-7 (dashed lines), a low-expressing cell line, and Jurkat (solid lines), a high-expressing cell line. The calculated signal-to-noise ratio is also shown. Custom conjugation avoids DIY conjugation inefficiencies such as suboptimal labeling, interference with specificity, and/or destabilization/degradation that negatively affect antibody performance and decrease yields