No, you should use a FULL bottle of 100 mL concentrate per 400 mL of water to guarantee that your newly mixed bottle of COOL MINT ZERO is just as effective as our regular COOL MINT ZERO. It is important to carefully read and follow the directions on the label to obtain the proper concentration. Directions for how to dilute or mix the concentrate to refill your empty bottle of COOL MINT ZERO can be found on the back of the smaller concentrate refill bottle (100 mL). You must use one FULL bottle of 100 mL of concentrate and 400 mL of water.