From a property law point of view, you can only remove them once your spouse is no longer legally able to live there--i.e. you need to deal with the marital situation first. That is because anyone who may legally live someplace may have guests: as long as your wife has the right to reside in the home, she may have her children live with her.If and when your spouse no longer resides there, then you can remove them: guests may not stay once the only person(s) who have legal possession of a home want them gone. If they don't go voluntarily at that point, you could bring a legal action commonly called "ejectment" (your state may have a different term for it) to remove them. A landlord-tenant lawyer should know how to file this action and you are advised to let such a lawyer help you: it can be a "technical" action in that a minor paperwork or procedural mistake can force you to start over.If you are threatened or assaulted, you may be able to get a protective or restraining order against them that will effectively require the to leave. If they uttered any threats, file a police report and ask the PD about the process for filing for a protective order--they can likely give you instructions or directions that will let you do this yourself (or point you to an agency or service that can help).