Sequence data (raw read) QC is done by using the FastQC quality check tool. This tool has been widely used in various publications and has greater acceptance in the scientific community for FASTQ file quality check. The quality check is based on the sequence quality of the individual bases in the reads and the reports are generated accordingly. For more details, please refer to https://www.bioinformatics.babraham.ac.uk/projects/fastqc/