Yes! Gluten can be hidden in ingredients in store bought dry rubs. That's why it's always safest to make your own from scratch. That way you control the ingredients and can insure it fits within your dietary needs. Individual herbs and spices are generally gluten free to begin with. But when you get those store-bought combinations they can be hidden in the form of an anti-caking agent. These can also contain wheat based ingredients. So if you're sensitive to gluten, your safest bet is to make your own seasonings and dry rubs from scratch.