Vo filme American Virgin hrajú Jenna Dewan (Priscilla White), Rob Schneider (Ed Curtzman), Chase Ryan Jeffery (Chuck), Brianne Davis (Naz), Elan Moss-Bachrach (Kevin), Ashley Schneider (Eileen), Bo Burnham (Rudy), Ben Marten (Brad), Bridgette Pechman (Mary Margaret), Michael Dailey (Harold), Jean Lyle Lepard (Bernice), Sarah Habel (Becca Curtzman), Samantha Moltmaker (Sandra), Ruth Crawford (School Administrator), Jacquelyn Evola (Young Priscilla White), David Fletcher (Walk of Shame Prankster), Andrea Langmeyer (Hot Girl #1), Barbara Dacko (College Girl #1), Brian Matthew Richardson (Drunk Man), Tom Whalen (Minister), Alisha Gellin (Car Sex Girl), Duncan Hursley (Steve), Alexander Zikakis (Nathan), Kyra D. Jackson (Sorority Girl #1), Bettina Beckman (Sorority Girl #2), Brandan Gugula (Stripper Guy), Andrea Harrison (Inane Girl), Connell Brown (Warm Up Guy), Renee Scavone (Street Party Girl #1), Stephanie Scarletta (Street Party Girl #2), Karan Batta (Bartender), Gary Wojnar (Father at Purity Dance), Ron Causey (Beer Vendor (uncredited)), Scott Joel Gizicki (Guy at Bar (uncredited)), Brad Leo Lyon (Street Partier (uncredited)), Garrett Thierry (Oktoberfest Partier (uncredited)), Amanda Bright (Sexy Party Girl #2 (uncredited)), Anna Dutcher (Oktoberfest Girl (uncredited)), Jodi Gabrielle Beals (Dancer / Bar Patron (uncredited)).