Yes. Creating multiple hosted zones allows you to verify your DNS setting in a “test” environment, and then replicate those settings on a “production” hosted zone. For example, hosted zone Z1234 might be your test version of example.com, hosted on name servers ns-1, ns-2, ns-3, and ns-4. Similarly, hosted zone Z5678 might be your production version of example.com, hosted on ns-5, ns-6, ns-7, and ns-8. Since each hosted zone has a virtual set of name servers associated with that zone, Route 53 will answer DNS queries for example.com differently depending on which name server you send the DNS query to.