With improved EBV laboratory tests, a faster and more accurate diagnosis could lead to a better prognosis and more effective treatment. In situ hybridization (ISH) of EBV-encoded RNA (EBER) in biopsy tissues remains the gold standard for detecting EBV in histopathological lesions. Methods for detecting EBV in various types of samples include the heterophile antibody test, immunofluorescence assays, enzyme immunoassays, Western blot, and polymerase chain reaction (PCR). However, detecting EBV viral load via PCR is gaining popularity in the diagnosis of EBV-associated diseases.