Cost for stable cell line generation can vary significantly across different projects. Here are two example projects and estimated  costs for each of them:Polyclonal overexpression in standard cell lineOverexpression of a low-risk target in a standard cell line with established transfection protocols. Detection of the target by flow cytometry using a commercially available antibody. Generation of polyclonal pools only and banking of 15 Mio cells.Estimated costs: 3 000–4 000€Monoclonal reporter cell line in a non-standard cell lineDesign of a reporter construct. Optimization of transfection protocols. Integration and validation of the reporter construct using 3 reference compounds. Assay establishment and optimization. Generation of dose-response curves as part of the cell line characterization. Generation of monoclonal cells and banking of a 150 Mio cell research cell bank.Estimated costs: 15 000–25 000€Please note that these examples are provided for reference only and pricing of your project may vary.