Quantifying amyloid plaques in rodent models presents challenges related to variability in plaque distribution, staining intensity, and background noise in IHC and IF images. IHC images are also subject to folds, tears, and artifacts that can interfere with the accurate quantification of amyloid burden. Developing standardized protocols for optimized tissue processing, antibody dilutions, staining protocols, and image acquisition parameters can help minimize variability and ensure reproducibility across studies. Collaborative efforts, such as inter-laboratory comparisons and proficiency testing, can also help to establish consensus protocols and guidelines for accurate plaque quantification and data interpretation.