ioSkeletal Myocytes are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media. By day three post-revival, ioSkeletal Myocytes demonstrate classical myocyte morphology and express the myocyte genes DES, MYOG, and myosin heavy chain, as assessed by quantitative reverse transcription PCR (qRT-PCR). By day seven post-revival, skeletal myocytes demonstrate expression of GLUT4 in peri-nuclear regions and striations. Skeletal myocytes express the major proteins of myofilaments, including myosin heavy chain, desmin, dystrophin, and troponin. By day 10 post-revival, form-striated, multinucleated myocytes that contract in response to acetylcholine are present.