Don't remove the collar, except for when bathing your dog and using shampoo.
Leave the collar on 24 hours a day. Clinical trials show that the collar is effective when used continuously (24 hours a day) as this allows your dog to continuously perceive the “appeasing messages” of ADAPTIL.
Once the collar is out of the box and the packet opened it has a 30 day life span. It is beneficial to keep it on your dog at all times and not remove it during these 30 days.