The PneumaCult product line consists of serum- and bovine pituitary extract (BPE)-free culture media for culturing human airway epithelial cells. PneumaCult-Ex Plus Medium is the most suitable choice for expanding primary human bronchial epithelial cells (HBECs) or human small airway epithelial cells (HSAECs) in 2D adherent culture. To differentiate HBECs or HSAECs at the air-liquid interface (ALI), use PneumaCult-ALI Medium or PneumaCult-ALI-S Medium, respectively. Differentiation of HBECs to three-dimensional (3D) airway organoids can be achieved using PneumaCult Airway Organoid Kit. Learn more about PneumaCult culture media for airway epithelial cells