We have found the best results from plating approximately
50,000 cells in 500ul media per chamber of an 8-well chamber slide. We do not
use Attachment FactorTM or other ECM in this application. We do not
pre-heat the slides, as some protocols suggest.
Once the cells are distributed to the wells, allow the slide
to sit in the hood for 10-15 minutes to let the cells settle before placing it
in the 37°C/5%CO2
incubator.