Glowing golf balls usually last between eight to ten minutes. That means you should have plenty of backup balls or be able to recharge them during the game quickly. Keep in mind that each golf ball has a different effect once it stops glowing. The UV-powered balls will slowly dim before the light fully dies. However, the glow stick and LED golf balls will completely stop after a set time. Then you'll need to replace the batteries or the glow sticks to continue having the glow effect.