For the High Concentration hydrogels, it is not recommended.  The concentration of the ionic molecule in medium would be much higher than the dilution solution, so diluting VitroGel with cell culture medium may cause the gel to form too quickly and produce a chunky consistency. We recommend using VitroGel Dilution Solution to adjust the highly concentrated VitroGel.
Alternatively, the Ready-to-use VitroGel hydrogels are a better option for scientists who do not want to worry about dilution and only want to mix the hydrogel with cell suspension directly.