Gas Chromatography (GC) and Liquid Chromatography (LC) are distinct separation techniques. GC utilizes a gaseous mobile phase to separate volatile compounds, ideal for analyzing low molecular weight substances. In contrast, LC employs a liquid mobile phase and is suitable for a broader range of compounds, including biomolecules. GC is more effective for analyzing volatile and nonpolar compounds, while LC is versatile for polar and nonvolatile substances. GC often provides faster separations, while LC allows for a wider range of stationary phases and applications. Both techniques are valuable tools in analytical chemistry, each offering unique advantages based on the analyte's characteristics.