Note: The test cassette must be at room temperature before use, and the test must be operated at room temperature.   Place and soak the Patient Swab in the Sample Eluent for 10 seconds and then stir well by rotating the swab against the side of the vial 5 times. Remove the Patient Swab while squeezing the middle of the Sample Eluent Vial to remove the liquid from the swab. Discard the swab in biohazard waste. Firmly attach Dropper Lid to the top of the Sample Eluent Vial. Gently invert the Sample Eluent Vial five times just before applying the sample to the Test Cassette. Apply the extracted sample from the Sample Eluent Vial onto the charging hole of the Test Cassette. To do this, gently press the sides of the Sample Eluent Vial to add 4 drops (about 80μL) of extracted sample to the charging hole of the Test Cassette. The sample will then be drawn by capillary action into the Test Strip. Start and timer and read the results after 10 minutes. The result is invalid after 15 minutes. Dispose the Swab, Sample Eluent Vial and Test Cassette in the appropriate biohazard waste.