Generally, our ELISA lab can perform sample analysis from 2-to-4 96-well plates within a typical workday if we have ELISA method development and immunoassay validation completed already. That said, sample preparation can vary per the complexity of the biological matrix and ELISA analysis itself (e.g., tissues or whole cells vs. serum). Similarly, sample incubation times can vary, typically overnight or less. Our scientists closely monitor and control the incubation and readout times during ELISA Method Development and sample analysis for consistency in your ELISA immunoassay results. Since the ELISA assay readouts are enzyme-dependent, lab personnel should read plates in the optimal reaction time window as the signal can decay markedly over time.