GE Silicone 2 sealant and GE Silicone 1 sealant will not freeze in the cartridge; however, for best results, it should be applied at room temperature. However, water-based acrylic caulks and sealants will freeze below 32° F. If the acrylic caulk or sealant freezes before you use it, simply thaw at room temperature and test before application. In either case, if you are concerned, make sure you test the caulk before application.