Vo filme Superman/Batman: Public Enemies hrajú Tim Daly (Superman (voice)), Kevin Conroy (Batman / Bruce Wayne (voice)), Clancy Brown (Lex Luthor (voice)), LeVar Burton (Black Lightning (voice)), Xander Berkeley (Captain Atom (voice)), Ricardo Chavira (Major Force (voice)), John C. McGinley (Metallo (voice)), CCH Pounder (Amanda Waller (voice)), Jennifer Haleová (Starfire / Killer Frost (voice)), Allison Mack (Power Girl (voice)), Corey Burton (Captain Marvel / Solomon Grundy (voice)), Calvin Tran (Toyman (voice)), Mark Jonathan Davis (Newscaster (voice)), Brian George (Gorilla Grodd (voice)), Rachael MacFarlane (Nightshade / Lady Shiva (voice)), Alan Oppenheimer (Alfred Pennyworth (voice)), Andrea Romano (Giganta / Computer (voice)), Bruce Timm (Mongul (voice)), Jonathan Adams (General (voice) (uncredited)), Michael Gough (Hawkman / Captain Cold (voice)).