Depending on who it is you wish to disinherit, the answer varies.
In Florida, you may leave your spouse out of your will, but unless you have signed a valid prenuptial or postnuptial agreement your spouse will be entitled to what is called the elective share. In Florida the spouse would be entitled to 30% of your estate through this elective share. If your spouse is going to take an elective share of your estate this request must be filed within 6 months of the date of service of a copy of the notice of administration or within 2 years after the date the decedent died. This request must be filed with the county where the estate is probated.