Yes there is a risk associated when reducing volumes unless you consider using a fit for purpose system. For precision liquid handling at very low volumes, automated liquid handlers like the mosquito HV genomics and dragonfly discovery use true positive displacement technology. Here, the plunger comes in direct contact with the reagent and liquid dispensing is unaffected by viscosity and air pressure. This style of dispensing is suited to additive kit protocols and uses a lower dead volume of reagent than others.