Adjusting the mixing ratio between the VitroGel solution and cell culture medium would change the hydrogel formation rate.
If the hydrogel solidifies too fast after mixing with the culture medium (showing as small solid gel chunk), adjust the mixing ratio by using less cell culture medium. For example, if mixing 2 mL hydrogel solution with 0.5 mL cell culture medium leads to solid gel chuck (particles), then mixing 2 mL diluted hydrogel solution with 0.2-0.4 mL cell culture medium would help to solve the issue.
On the other hand, if the hydrogel formation is too slow, adjust the mixing ratio by using more cell culture medium. For example, if mixing 2 mL diluted hydrogel solution with 0.5 mL cell culture medium leads to a slow hydrogel formation, then mixing 2 mL diluted hydrogel solution with 1-2 mL cell culture medium would help to solve the issue.