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</style></head> <body> text<div class="container"> <h1>Enzyme Inhibition Analysis</h1>
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<h2>Types of Reversible Inhibition</h2>
<table>
    <thead>
        <tr>
            <th>Inhibition Type</th>
            <th>Where Inhibitor Binds</th>
            <th>Effect on Kₘ</th>
            <th>Effect on Vₘₐₓ</th>
        </tr>
    </thead>
    <tbody>
        <tr>
            <td class="type-label">Competitive</td>
            <td>Active Site</td>
            <td class="increase"><span class="effect-arrow">↑</span> Increases</td>
            <td class="no-change">No Change</td>
        </tr>
        <tr>
            <td class="type-label">Uncompetitive</td>
            <td>Enzyme-Substrate (ES) Complex Only</td>
            <td class="decrease"><span class="effect-arrow">↓</span> Decreases</td>
            <td class="decrease-vmax"><span class="effect-arrow">↓</span> Decreases</td>
        </tr>
        <tr>
            <td class="type-label">Non-competitive</td>
            <td>Allosteric Site (on E or ES complex equally)</td>
            <td class="no-change">No Change</td>
            <td class="decrease-vmax"><span class="effect-arrow">↓</span> Decreases</td>
        </tr>
        <tr>
            <td class="type-label">Mixed</td>
            <td>Allosteric Site (on E or ES complex unequally)</td>
            <td><span class="increase">↑</span> or <span class="decrease">↓</span> (Can Increase or Decrease)</td>
            <td class="decrease-vmax"><span class="effect-arrow">↓</span> Decreases</td>
        </tr>
    </tbody>
</table>

<h2>Case Study 1: PFK-1 from Human Skeletal Muscle</h2>
<table>
    <thead>
        <tr>
            <th>Inhibition Type</th>
            <th>Where Inhibitor Binds</th>
            <th>Effect on Kₘ</th>
            <th>Effect on Vₘₐₓ</th>
        </tr>
    </thead>
    <tbody>
        <tr>
            <td class="type-label">Mixed</td>
            <td>Allosteric Site</td>
            <td class="increase"><span class="effect-arrow">↑</span> Increases (from 0.8 mM to 1.5 mM)</td>
            <td class="decrease-vmax">Decreases (Implied)</td>
        </tr>
    </tbody>
</table>
<div class="explanation">
    <strong>Passage Explanation:</strong> The passage states that citrate is a "negative allosteric effector" that was found to be "increasing the apparent Kₘ for F6P to 1.5 mM". Since Vₘₐₓ must decrease for inhibition to occur and Kₘ increases, this pattern is characteristic of <strong>mixed inhibition</strong>.
</div>


<h2>Case Study 2: PFK-1 from Rabbit Skeletal Muscle</h2>
<table>
    <thead>
        <tr>
            <th>Inhibition Type</th>
            <th>Where Inhibitor Binds</th>
            <th>Effect on Kₘ</th>
            <th>Effect on Vₘₐₓ</th>
        </tr>
    </thead>
    <tbody>
        <tr>
            <td class="type-label">Non-competitive</td>
            <td>Allosteric Site</td>
            <td class="no-change">No Change (0.15 ± 0.01 vs 0.15 ± 0.02)</td>
            <td class="decrease-vmax"><span class="effect-arrow">↓</span> Decreases (from 120 ± 5 to 60 ± 4)</td>
        </tr>
    </tbody>
</table>
<div class="explanation">
    <strong>Passage Explanation:</strong> The data shows that with the addition of 50 µM citrate, Vₘₐₓ is halved (from 120 to 60 µmol min⁻¹ mg⁻¹) while Kₘ remains statistically unchanged (0.15 mM). This combination of a decreased Vₘₐₓ and an unchanged Kₘ is the classic definition of <strong>non-competitive inhibition</strong>.
</div>
</div> </body> </html> Keep the dark mode, but give a final HTML, CSS, JavaScript that is much nicer to look at, and it's much easier to see the distinctions, and the tables are drawn much prettier.
give full final html in code box only --- Improve this to be much better quality have better design tables and also explain why mix inhibition has the plus or minus don't just state it.