mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Prevalence of depressive and anxiety disorders in Chinese gastroenterological outpatients

AIM:To investigate the prevalence and physicians’ detection rate of depressive and anxiety disorders in gastrointestinal(GI)outpatients across China. METHODS:A hospital-based cross-sectional survey was conducted in the GI outpatient departments of 13 general hospitals.A total of 1995 GI outpatients were recruited and screened with the Hospital Anxiety and Depression Scale(HADS).The physicians of the GI departments performed routine clinical diagnosis and management without knowing the HADS score results.Subjects with HADS scores≥8 were subsequently interviewed by psychiatrists using the Mini International Neuropsy-chiatric Interview(MINI)to make further diagnoses. RESULTS:There were 1059 patients with HADS score ≥8 and 674(63.64%)of them undertook the MINI interview by psychiatrists.Based on the criteria of Diagnostic and Statistical Manual of Mental Disorders (4th edition),the adjusted current prevalence for depressive disorders,anxiety disorders,and comorbidity of both disorders in the GI outpatients was 14.39%, 9.42%and 4.66%,respectively.Prevalence of depressive disorders with suicidal problems[suicide attempt or suicide-related ideation prior or current;module C (suicide)of MINI score≥1]was 5.84%in women and 1.64%in men.The GI physicians’detection rate of depressive and anxiety disorders accounted for 4.14%. CONCLUSION:While the prevalence of depressive and anxiety disorders is high in Chinese GI outpatients, the detection rate of depressive and anxiety disorders by physicians is low.
Efficacy of mosapride citrate with polyethylene glycol solution for colonoscopy preparation

AIM:To evaluate the efficacy and safety of adjunctive mosapride citrate for bowel preparation before colonoscopy. METHODS:We conducted a randomized,double-blind, placebo-controlled study with mosapride in addition to polyethylene glycol(PEG)-electrolyte solution.Of 250 patients undergoing colonoscopy,124 were randomized to receive 2 L PEG plus 15 mg of mosapride citrate (mosapride group),and 126 received 2 L PEG plus placebo(placebo group).Patients completed a questionnaire reporting the acceptability and tolerability of the bowel preparation process.The efficacy of bowel preparation was assessed by colonoscopists using a 5-point scale based on Aronchick’s criteria.The primary end point was optimal bowel preparation rates(scores of excellent/good/fair vs poor/inadequate). RESULTS:A total of 249 patients were included in the analysis.In the mosapride group,optimal bowel preparation rates were significantly higher in the left colon compared with the placebo group(78.2%vs 65.6%,P<0.05),but not in the right colon(76.5%vs 66.4%,P=0.08).After excluding patients with severe constipation,there was a significant difference in bowel preparation in both the left and right colon(82.4%vs 66.7%,80.8%vs 67.5%,P<0.05,P<0.01).The incidence of adverse events was similar in both groups. Among the subgroup who had previous colonoscopy experience,a significantly higher number of patients in the mosapride group felt that the current preparation was easier compared with patients in the placebo group(34/72 patients vs 24/74 patients,P<0.05). CONCLUSION:Mosapride citrate may be an effective and safe adjunct to PEG-electrolyte solution that leads to improved quality of bowel preparation,especially in patients without severe constipation.
《傅青主女科》益经汤对治疗卵巢早衰的启示

月经的产生以肾为根本,多数医家从肾论治卵巢早衰,然而随着社会的发展,社会角色的转变,妇女社会压力越来越大,现代研究表明,卵巢早衰与情志关系密切,但古代医家及现代医家对之论述不多。《傅青主女科》从"郁"论治,认为本病的病机为"气郁为病",首创"柔肝解郁法",提出了解郁重在养血育阴、益气健脾的新见解,对于治疗卵巢早衰有重要的指导意义。
波谱成像联合弥散成像诊断老年缺血性脑病研究进展

缺血性脑病是指由于心脏骤停、低血压、失血、低血糖、窒息等原因引起脑血流下降到低于10~15 ml.100 g~(-1).min~(-1)而引起的脑损伤。缺血性脑病有以下几种类型:①层状坏死,大脑皮质神经元分层坏死、脱失、胶质化,使皮质神经细胞层中断;②海马锥体细胞损伤、脱失、胶质化;③边缘带梗死,缺血性脑病的临床症状与缺血的严重程度和持续时间有关，症状轻的患者有一过性精神错乱，上肢肩带肌力，感觉减退等症状，病情严重的患者可昏迷死亡，成为植物人。使用传统磁共振成像（MRI）诊断缺血性脑病早期很难被发现，缺血性脑实质的范围只有在较晚时期当血管源性水肿出现时才能被发现。尽早诊断和治疗缺血性脑病具有重要的理论和现实意义。结合常规MRI、弥散加权成像（DWI）可以推断脑缺血所处的时期，磁共振波谱成像（MRS）能提供脑缺血区代谢改变，联合应用两种成像技术对缺血性脑病预测和救治有一定帮助。
地佐辛对老年胃肠道肿瘤患者术后芬太尼镇痛效果的影响

胃肠道肿瘤术后的急性疼痛对患者的生理及心理均会产生许多不利影响,尤其是老年患者。术后镇痛常规以阿片类药物为主,对于老年患者来说,纯阿片类药物镇痛可能会带来许多副作用,因此需要找寻合适的配方,以期达到镇痛好,副作用少,患者经济负担小的目的。地佐辛(dezocine)是阿片受体激动-拮抗镇痛药，主要激动κ-阿片肽受体，对μ受体具有激动拮抗作用，本研究通过地佐辛与阿片类镇痛药芬太尼对老年胃肠道患者术后镇痛效果的比较，探寻最适宜的镇痛方案。
经尿道前列腺电切术后排尿困难25例

近年来,经尿道前列腺电切术(TURP)已被公认为治疗良性前列腺增生疾病的标准治疗手段。与传统的开放性手术相比,TURP具有创伤小、恢复快、手术时间短、住院时间短等优点,但是有些术中术后的并发症仍旧困扰着广大泌尿外科医生,也影响了这一标准治疗手段的广泛开展,其中术后再次出现排尿困难发生率较高。我院自2001年5月开始采用TURP治疗良性前列腺增生症,现将2011年收治的TURP术后再次出现排尿困难的25例患者的诊治体会分析如下。
老年血液透析患者外周血单个核细胞核因子-κB p65的活性及临床评价

目的观察老年维持性血液透析(MHD)患者外周血单个核细胞(PBMC)核因子-κB p65(NF-κB p65)的活性及其与超敏C反应蛋白(hs-CRP)水平和营养状况的关系。方法选择MHD治疗3个月以上、尿素清除指数(Kt/V)>1.2的患者71例。老年组(≥60岁)35例,非老年组(<60岁)36例。采用改良主观综合营养评估(MQSGA)法、血生化及人体学评估营养状况。免疫透射比浊法检测血清hs-CRP水平。ELISA法检测PBMC中NF-κBp65的活性。相关性分析采用Pearson直线相关和偏相关。结果①老年组发生营养不良者29例(82.86%),显著高于非老年组22例(61.11%)(P<0.05);老年组MQSGA积分(14.97±5.98)显著高于非老年组(11.89±2.93)(P<0.01);老年组血清白蛋白(ALB)、前白蛋白(PA)、透析前肌酐(Scr)、透析前尿素氮(BUN)均显著低于非老年组(P<0.01或P<0.001)。老年组发生微炎症者20例(57.14%),显著高于非老年组10例(27.78%)(P<0.05);老年组hs-CRP水平〔(5.03±4.13)mg/L〕、NF-κB活性〔(0.84±0.33)OD〕显著高于非老年组〔(2.92±3.44)mg/L和(0.61±0.36)OD〕(P<0.05和P<0.01)。老年组标准化蛋白分解率(nPCR)为(0.89±0.19)g.kg~(-1).d~(-1),显著低于非老年组(1.03±0.21)g.kg~(-1).d~(-1)(P<0.01)。②Pearson直线相关和控制年龄、性别、身高、体质量影响的偏相关分析结果均显示,老年MHD患者NF-κB活性与hs-CRP、MQSGA呈正相关(P<0.001、P<0.05),与ALB呈负相关(P<0.05)。结论老年MHD患者营养不良发生率高,程度重。NF-κB活性与hs-CRP显著正相关,其异常活化可能是影响老年MHD患者营养不良的重要危险因素之一。检测NF-κB活性可预测营养不良。
自制监护仪血压袖带外套防止交叉感染

我们根据监护仪血压袖带内胆可取出、外套可消毒清洗,自制了许多与原规格一致但侧边未封口的备用袖带外套用于更换消毒,使用后效果良好,不影响血压监测结果。
珍汕97B改良新不育系与珍汕97A的制种产量比较研究

以6个珍汕97B改良新不育系珍品A、珍农567A、珍农711A、珍农256A、珍农257A、珍农060A为母本,珍汕97A为对照,以48-2为父本,进行了杂交制种产量比较试验。结果表明,与对照(珍汕97A/48-2)相比,不育系珍农257A、珍农256A、珍品A和珍农567A分别与48-2配制F1的制种产量极显著增加,平均产量分别为3.13,3.07,3.06和2.97 t/hm~2,分别比对照增产14.31%,12.36%,11.81%和8.45%;这4个不育系柱头外露率和异交结实率均高于珍汕97A是制种产量显著提高的主要原因。
子宫上皮样滋养细胞肿瘤1例

ETT缺乏特异性临床表现,有前次妊娠史,不规则阴道流血,轻度HCG升高和对化疗不敏感等,均提示ETT。但ETT的诊断和鉴别诊断主要根据病理检查和免疫组化染色结果。ETT治疗尚未达成共识。由于其起源于中间滋养细胞,对传统化疗不敏感,病灶位于子宫者,多主张采用全子宫切除术,多可保留双侧附件,术后辅以化疗。目前尚无可靠的预后评估指标。
高纯铝表面电抛光形成自组织图案的研究进展

近年来具有高度有序的自组织结构的多孔阳极氧化铝(AAO)膜得到广泛的应用,尤其是作为制备纳米材料的模板.传统的光刻技术主要包括紫外光刻或X射线光刻、电子束光刻和离子束光刻。以电子束光刻为例,与自组织技术相比，电子束光刻在纳米尺度范围内有以下几个缺点。例如制备和复制掩模的价格昂贵，并且由于曝光、焙烧和刻蚀等过程容易发生失误使得产率较低。在纳米尺寸分界面上，玻璃化温度，分散系数和光刻胶的溶解速率因主体材料特性的不同而不同。与传统的光刻技术相比，自组织是一种价格低廉并有效形成二维纳米超点阵有序阵列的过程。铝阳极氧化和电化学抛光是两种可产生有序二维点阵的自组织技术。
截尾正态分布的随机抽样和R软件的实现

统计学中所使用的数据大概可分为真实数据和模拟数据，模拟数据是所有Monte Carlo研究的基础，又称伪数据(pseudo data)〔1〕。几乎所有统计软件都提供了常用分布(如正态、均匀和beta分布等)的随机数生成器，应用这些函数在变量的整个样本空间抽样很容易，但要想在分布的部分取值范围内(比如截尾分布)抽样却有些困难，此时或者需要复杂的数学转换，或者需要考虑抽样的效率问题〔2〕。所谓截尾分布(truncated distribution)是指变量取值被限制在特定范围而不能在整个支撑上取值〔2〕。如果只能取小于某个数a的值称为右截尾(right truncated)，如果只能取大于某个数b的值称为左截尾(left truncated)，如果取值范围限定在一个区间[a，b]称之为区间截尾(interval truncated)，a，b称为截点或阈值。显然，[- ∞，a]即为右截尾，[b，∞]即为左截尾，因此右(左) 截尾是区间截尾的特例。
上海市浦东新区主要恶性肿瘤疾病负担研究

目的对2002~2005年浦东新区主要恶性肿瘤情况分析和比较，评价其疾病负担。方法用WHO疾病负担研究方法评价恶性肿瘤疾病负担。结果男女性结直肠癌、男性肺癌的YLL、YLD、DALY均呈上升趋势，男性食管癌、肝癌、女性食管癌、肝癌、肺癌的YLL均变化不明显，由于YLL所占构成均比较大，YLD有变化但影响不大，导致DALY也变化不明显。男女性胃癌的YLD都呈上升趋势，但男性DALY呈下降趋势，女性则呈上升趋势。女性乳腺癌YLL增长明显，导致DALY上升。结论浦东新区的老龄化程度较高，人口负担较重；浦东新区男女性结直肠癌、男性肺癌、女性乳腺癌、胃癌的疾病负担呈上升趋势；男性食管癌、肝癌、女性食管癌、肝癌、肺癌的疾病负担变化不明显；男性胃癌DALY呈下降趋势。应继续加强恶性肿瘤监测工作；加强恶性肿瘤监测信息的利用；全面开展恶性肿瘤的三级预防。
珠海市居民健康素养多因素统计模型

目的了解珠海市居民健康素养影响因素。方法应用整群抽样方法在珠海市香洲、斗门和金湾3个行政区内分别抽取6个居委会和6个行政村15~69岁人群进行健康素养问卷调查，对珠海市居民健康素养影响因素进行综合分析。结果健康知识因子的大小与调查对象的性别、文化程度、职业和家庭收入有关；健康理念因子与调查对象的年龄、文化程度、职业和购买保险有关；健康行为因子与调查对象的性别、职业、家庭收入和身体疾病状况有关；健康技能因子与身体的疾病状况有关。结论珠海市居民健康素养水平主要受性别、文化程度、职业、家庭收入、疾病、年龄等因素的影响。
Spatial Variability of Soil Organic Carbon and Related Factors in Jiangsu Province,China

Soil organic carbon (SOC) plays a key role in the global carbon cycle.In this study,we used statistical and geostatistical methods to characterize and compare the spatial heterogeneity of SOC in soils of Jiangsu Province,China,and investigate the factors that influence it,such as topography,soil type,and land use.Our study was based on 24 186 soil samples obtained from the surface soil layer (0-0.2 m) and covering the entire area of the province.Interpolated values of SOC density in the surface layer,obtained by kriging based on a spherical model,ranged between 3.25 and 32.43 kg m~(-3).The highest SOC densities tended to occur in the Taihu Plain,Lixia River Plain,along the Yangtze River,and in high-elevation hilly areas such as those in northern and southwest Jiangsu,while the lowest values were found in the coastal plain.Elevation,slope,soil type,and land use type significantly affected SOC densities.Steeper slope tended to result in SOC decline.Correlation between elevation and SOC densities was positive in the hill areas but negative in the low plain areas,probably due to the effect of different land cover types,temperature,and soil fertility.High SOC densities were usually found in limestone and paddy soils and low densities in coastal saline soils and alluvial soils,indicating that high clay and silt contents in the soils could lead to an increase,and high sand content to a decrease in the accumulation of SOC.SOC densities were sensitive to land use and usually increased in towns,woodland,paddy land,and shallow water areas,which were strongly affected by industrial and human activities,covered with highly productive vegetation,or subject to long-term use of organic fertilizers or flooding conditions.
Impacts of Soil Additives on Crop Yield and C-Sequestration in Post Mine Substrates of Lusatia,Germany

Opencast lignite mining in the Lusatia region of Germany has resulted in large scale landscape disturbances,which require suitable recultivation techniques in order to promote plant growth and establishment in the remaining nutrient-poor substrates with low water-holding capacity.Thus,the effects of two commercial soil additives (CSA),a hydrophilic polymer mixed with volcanic rock flour and bentonite (a-CSA),and digester solids from biogas plants enriched with humic acids and bentonite (b-CSA),on soil organic carbon (SOC) storage,plant yields and root biomass were assessed after cultivating perennial crops (Dactylis glomerata L.) in monoculture and Helianthus annuus L.-Brassica napus L.in crop rotation systems.The CSA were incorporated into the top 20 cm soil depth using a rotary spader.The results indicated that a-CSA led to a significant increase in plant yield during the first year,and improved root biomass in the following year.As a result,SOC stocks increased,especially in the 0-10 cm soil layer.No significant sequestration of additional SOC was observed on b-CSA-amended plots at the end of both years.Bulk density values decreased in all treatments under the monoculture system.It can be concluded that application of a-CSA enhanced soil water availability for plant uptake and consequently promoted plant growth and organic carbon sequestration.The relative enrichment of organic matter without effects on water-holding capacities of b-CSA treatments suggested that it was not suitable for rapid land reclamation.
Mapping Soil Texture of a Plain Area Using Fuzzy-c-Means Clustering Method Based on Land Surface Diurnal Temperature Difference

The use of landscape covariates to estimate soil properties is not suitable for the areas of low relief due to the high variability of soil properties in similar topographic and vegetation conditions.A new method was implemented to map regional soil texture (in terms of sand,silt and clay contents) by hypothesizing that the change in the land surface diurnal temperature difference (DTD) is related to soil texture in case of a relatively homogeneous rainfall input.To examine this hypothesis,the DTDs from moderate resolution imagine spectroradiometer (MODIS) during a selected time period,i.e.,after a heavy rainfall between autumn harvest and autumn sowing,were classified using fuzzy-c-means (FCM) clustering.Six classes were generated,and for each class,the sand (> 0.05 mm),silt (0.002-0.05 mm) and clay (< 0.002 mm) contents at the location of maximum membership value were considered as the typical values of that class.A weighted average model was then used to digitally map soil texture.The results showed that the predicted map quite accurately reflected the regional soil variation.A validation dataset produced estimates of error for the predicted maps of sand,silt and clay contents at root mean of squared error values of 8.4%,7.8% and 2.3%,respectively,which is satisfactory in a practical context.This study thus provided a methodology that can help improve the accuracy and efficiency of soil texture mapping in plain areas using easily available data sources.
Use of Spent Mushroom Substrate as Growing Media for Tomato and Cucumber Seedlings

The aim of this research was to evaluate weathered spent mushroom substrate (SMS),made from spent Flammulina velutipes mushroom substrate,as a growing medium for nursery seedlings.Two vegetable species,cucumber (Cucumis sativus L.cv.Jinchun No.2) and tomato (Solanum lycopersicum L.cv.Mandy),were grown in 8 media of SMS in various ratios with perlite or vermiculite.A mixed substrate of peat with perlite (1:1;v:v) was used as the control (CK).The experiment was arranged in a completely randomized design under greenhouse conditions.Prior to sowing,some physical and chemical properties of the growing media were determined.Results showed that all the mixtures had desirable physical and chemical properties for their use in nursery tomato and cucumber seedlings except for the T4 (SMS:vermiculite=1:1;v:v) and the T8 (SMS:perlite=2:1;v:v) mixtures.Compared with the CK,increased plant height,leaf area,fresh weight,dry weight and index of seedling quality were found in the T3 (SMS:vermiculite=2:1;v:v) and T6 (SMS:perlite=4:1;v:v) growing media.SMS should be considered as an alternative for the widely used but expensive and resource-limited peat in greenhouse cultivation.
Responses of Iron-Reducing Bacteria to Salinity and Organic Matter Amendment in Paddy Soils of Thailand

In paddy soils of Thailand,the addition of organic matter (OM) is used to efficiently limit the effect of salinity on rice culture and production.OM used as an amendment and fertilizer promotes the reduced condition and increases iron solubilization without provoking ferrous toxicity.In this study,the intricate biogeochemical role of iron-reducing bacteria (IRB) involved in the quality of water and soil of paddy fields,particularly when the paddy fields were subject to salinity and organic matter addition,were studied in paddy fields of Thailand.The results demonstrated that the addition of OM increased the proliferation of cultivable IRB and their specific activity.Cultivable IRB communities decreased in the presence of salt.The presence of salt modified the structure of the bacterial populations by favoring the development of alkaline and moderately halophilic bacteria (Virgibacillus spp.,Oceanobacillus spp.,and Paenibacillus spp.).The paddy soils studied contained very diversified (halosensitive,halotolerant,and halophilic) IRB populations that could be adapted to a variety of salinity conditions (0-90 g L 1 NaCl) using different organic substrates (glucose,acetate,and soil organic matter) to maintain significant activities under extreme conditions of salinity.The rhizosphere of rice stimulated IRB community growth without organic matter,whereas organic matter addition limited the rhizosphere effect on IRB cultivable number in saline condition.The interactive action of salinity and organic amendment had a negative impact on the rhizosphere effect.The presence of specific iron-reducing populations (fermentative,iron-respiring,anaerobic,and facultative anaerobic),having different behaviors under salt and redox stresses,appeared to be a key factor that contributed to the control or enhancement of the quality of water and soil in paddy fields.
In-Season Root-Zone Nitrogen Management Strategies for Improving Nitrogen Use Efficiency in High-Yielding Maize Production in China

Many recently developed N management strategies have been extremely successful in improving N use efficiency.How-ever,attempts to further increase grain yields have had limited success.Field experiments were conducted in 2007 and 2008 at four sites to evaluate the effect of an in-season root-zone N management strategy on maize (Zea mays L.).According to the in-season root-zone N management,the optimal N rate (ONR) was determined by subtracting measured soil mineral N (NH_4~+--N and NO_3~--N) in the root zone from N target values.Other treatments included a control without N fertilization,70% of ONR,130% of ONR,and recommended N rate (RNR) by agronomists in China that have been shown to approach maize yield potentials.Although apparent N recovery for the ONR treatment was significantly higher than that under RNR in 2007,grain yield declined from 13.3 to 11.0 Mg ha~(-1) because of an underestimation of N uptake.In 2008,N target values were adjusted to match crop uptake,and N fertilization rates were reduced from 450 kg N ha~(-1) for RNR to 225 to 265 kg N ha~(-1) for ONR.High maize yields were maintained at 12.6 to 13.5 Mg ha~(-1),which were twice the yield from typical farmers’ practice.As a result,apparent N recovery increased from 29% to 66%,and estimated N losses decreased significantly for the ONR treatment compared to the RNR treatment.In conclusion,the in-season root-zone N management approach was able to achieve high yields,high NUE and low N losses.
阿魏酸钠治疗血管性痴呆46例

目的观察阿魏酸钠治疗血管性痴呆(VD)的临床疗效和安全性。方法92例VD患者分为对照组和观察组。对照组使用茴拉西坦、疏血通等常规治疗,观察组在上述常规治疗的基础上加用阿魏酸钠0.3 g+50g/L葡萄糖注射液250 mL静脉滴注,1次/d,10 d为一疗程,共治疗5个疗程。以简易智能状态检查量表(MMSE)、日常生活能力量表(ADL)、总体衰退量表(GDS)等评分作为主要评价指标,比较两组患者在治疗前及治疗后第4、8周认知能力、精神状况、日常生活能力的改善情况。结果(1)治疗4周后观察组MMSE、GDS评分明显优于对照组,差异有统计学意义(P<0.05),ADL评分差异无统计学意义(P>0.05)。治疗8周后观察组MMSE、ADL、GDS评分与对照组比较,差异均有统计学意义(P<0.05,P<0.01)。(2)临床疗效满意,观察组患者MMSE、ADL、GDS评分在第4、8周的总有效率均明显高于对照组,差异有统计学意义(P<0.01)。结论阿魏酸钠治疗VD安全有效。
TCRCα-560C/T基因多态性与原发抗中性粒细胞胞浆抗体相关性小血管炎的关系

目的探讨T细胞受体保守域α链基因(TCRCα)-560 C/T多态性与广西汉族人群中原发抗中性粒细胞胞浆抗体(ANCA)相关性小血管炎(AAV)的相关关系。方法PCR-RFLP鉴定81例AAV患者的基因型,与190例年龄、性别相匹配的汉族健康成人作对照,行病例-对照研究、临床及病理分析。结果(1)81例AAV患者TCRCα-560C/T存在3种基因型,CC∶TT∶CT=20∶25∶36,与健康或人(CC∶TT∶CT=45∶100∶45)比较,基因型及等位基因频率差异无统计学意义(P>0.05);(2)临床分析显示:3种基因型的患者,24 h尿蛋白定量和空腹血糖及发热发生率差异有统计学意义(P<0.05),TT基因型患者的24 h尿蛋白定量和空腹血糖均显著升高;CC基因型患者的发热发生率明显升高。(3)病理分析显示:3种基因型的患者肾脏病理活动性评分和慢性评分差异无统计学意义(P>0.05)。结论广西汉族人群中,TCRCα-560C/T基因多态性可能与AAV患者发热、24 h尿蛋白定量及空腹血糖升高相关,可能与AAV的遗传易感性不相关。
自制单腔双套囊支气管导管与双腔支气管导管用于单肺通气的效果对比

目的比较自制单腔双套囊支气管导管与双腔支气管导管用于胸外科单肺通气的麻醉效果。方法选择胸外科需在单肺通气下行择期手术患者100例,分为两组:观察组(n=50)使用自制单腔双套囊支气管导管,对照组(n=50)使用双腔支气管导管。记录插管时间、插管次数、肺隔离时间、平卧位时定位时间以及肺萎陷情况及术后声嘶和咽疼发生率。结果与对照组比较,观察组插管时间、插管次数、肺隔离时间及平卧位时定位时间均降低(P<0.05),而且肺萎陷良好,术野暴露满意,术后声嘶和咽疼发生率下降。结论在单肺通气中使用自制单腔双套囊支气管导管,麻醉效果与双腔支气管导管相近,具有插管方便、定位简单、双肺分隔完全、对患者生理干扰小等优点。
王辉武治疗痤疮经验

痤疮又名粉刺、暗疮,是一种临床常见病,好发于青春发育期男女,常常反复发作,顽固难愈。临床表现为粉刺、丘疹、脓疱、结节、囊肿及瘢痕等,好发于面、背、胸等部位,属于毁容性皮肤病[1-2],影响患者的身心健康。王老师以中医思维探讨痤疮的病因病机,在临床实践中独辟蹊径,总结出一套相对简便易行的治疗方案。我们有幸侍诊王老师左右,获益良多,现简述如下。
从“卫出下焦”探讨《伤寒论》对附子的运用

卫气根于下焦,卫气的生成、循行、发挥功能皆不离肾,卫气功能的强弱取决于肾气的盛衰。张仲景据"卫出下焦"理论创立温肾助卫、扶阳固表之治法,治疗卫气虚弱证或阳虚外感证,以(炮)附子温肾阳、充卫阳而固肌表。太阳底面,即是少阴,因此,在治疗太阳病的同时,须处处顾及少阴真阳。
补血益气方对运动员大负荷运动后体能恢复的影响

大负荷运动可引起运动员血红蛋白水平下降,影响运动员的训练状态和运动能力。补血益气方可以提高运动员的血红蛋白含量,显著降低肌酸激酶活性、降低血尿素水平和血乳酸水平,表明补血益气方可以促进大负荷运动后的恢复,促进疲劳的消除,增强运动员的运动能力。
从热、毒、痰、瘀互结论治中风病先兆证

中风病先兆证的研究对于中风病的防治具有重要意义,但是关于中风病先兆证的病机关键环节目前尚未达成共识。探讨了热毒的中医学内涵及热毒和痰浊瘀血之间的关系,从热毒痰瘀互结研究中风先兆证的病理基础及主要病机,并提出其治疗原则及方药。
微生物有机肥配施氮肥对烤烟SPAD值、烟叶酶活性及根系活力的影响

不同氮肥对烤烟生长的影响不同,有研究表明硝态氮较酰胺态有利于烤烟生长,硝态氮和铵态氮比例为1:1时效果最好,本项目中其他大田试验也得出相一致的结果。而该盆栽试验结果相反,单施尿素烟叶SPAD值、烟叶鲜重及酶活性、根系鲜重及根系氧化力和活力较单施硝铵磷高,其原因可能与栽培环境(温度、水分、光强度等)和混匀一次性施肥方式等有关。
Inflammatory responses of the rat lungs in cold-dryness syndrome in the northwest of China

OBJECTIVE:To examine changes in body weight and the lung inflammation factors interleukin-1β(IL-1β),interleukin-8(IL-8),IL-10 and tumor necrosis factor-α(TNF-α) in a rat model of cold-dryness syndrome in Northwest(Xinjiang) China to provide a reference for treating chronic obstructive pulmonary disease(COPD) with local peculiarities.METHODS:The rat COPD model was established by intratracheal instillation of porcine pancreatic elastase(PPE) in combination with cigarette smoking(CS).The rat model of cold-dryness syndrome of COPD in the northwest of China was set up by intratracheal instillation of PPE in combination with CS and environmental cold-dryness stress.The level of IL-1β,IL-8,IL-10 and TNF-α in Bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay(ELISA).The data were analyzed using the software SPSS 11.5.RESULTS:1) Body weight was less in the two model groups than that of control group(P<0.01),PPE plus CS cold-dryness group was less than that of PPE plus CS group(P<0.01).2) IL-1β in BALF significantly increased in PPE plus CS and cold-dryness group than that of control group(P<0.01).3) IL-8 and TNF-α in BALF significantly increased in PPE plus CS and cold-dryness group and PPE plus CS group than that of control group(P<0.01).CONCLUSION:Body weight in COPD model rats was reduced compared with controls.Cold-dryness may aggravate such a condition Lung inflammation in the model was mainly manifested by an increase in IL-1β,IL-8 and TNF-α levels,with no change in IL-10 levels.Cold-dryness may aggravate lung inflammation of COPD.
Applications of immuno-magnetic bead and immunofluorescent flow cytometric techniques for the quantitative detection of HAB microalgae

Overthelastseveraldecades,harmfulalgalblooms(HABs)havebecomeaseriousenvironmental problem in many parts of the world. A rapid and accurate detection process for HAB algae has yet to be developed. Heterosigma akashiwo is one of the most important HABs species in China. The objective of this study was to develop an immunologic technique that can rapidly and sensitively count H. akashiwo cells. Five HABs species (Alexandrium catenella, Thalassiosira sp., Cryptomonas sp., Alexandrium tamarense and Symbiodinium sp.,) were used in this study to evaluate the analysis process we developed. A polyclonal antibody with high titers against H. akashiwo was obtained by injecting H. akashiwo cells into rabbits. Immuno-magnetic beads (IMB) were produced via conjugated polyclonal antibodies with magnetic beads and applied to isolate and count H. akashiwo cells from the culture. Results show that 66.7%-91.6% of the cells were captured from unialgal culture by IMBs, and only 5.3%-12.5% of the four other HAB microalgae species were captured, indicating that the constructed IMBs combined specifically with the H. akashiwo cells. At the same time, flow cytometry (FCM) sorting was exploited to screen H. akashiwo cells after labeling with FITC conjugated polyclonal antibodies. Using the FCM technique, 91.7% of the targeted cells were sorted out from mixed microalgae samples in just a few minutes. These results indicate that both antibody-involved IMB and antibody-based FCM techniques are highly effective at detecting and quantifying HAB species. These techniques, especially immuno-magnetic separation, have low associated cost, and are fast and simple processes compared with other techniques currently in use.
BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.