mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Nemo样激酶在结直肠癌组织中的表达及其临床意义

目的研究Nemo样激酶(NLK)基因在结直肠癌组织中的表达情况及其临床意义。方法选取2001年1月至2009年12月间在上海第二军医大学附属长海医院肛肠外科行手术治疗患者的肠黏膜组织,其中正常黏膜组56例(行吻合器痔上黏膜环切钉合术的混合痔患者);结直肠腺瘤组51例;结直肠癌组712例;转移性结直肠癌组21例。将选取的肠黏膜组织标本制作成组织芯片,采用Envision免疫组织化学法检测NLK的表达情况,并分析NLK的表达水平与临床病理因素的关系。结果NLK的表达阳性率从正常黏膜(75.0%,42/56)、结直肠腺瘤(86.3%,44/51)到结直肠癌[I、Ⅱ、Ⅲ、Ⅳ期分别为92.5%(49/53)、90.1%(281/312)、91.9%(274/298)和98.0% (48/49)]依次升高。NLK的表达水平与结直肠癌淋巴结转移(P=0.027)、肿瘤远处转移(P=0.000)、肿瘤TNM分期(P=0.000)和复发率(P<0.01)呈正相关。NLK在低分化和黏液腺癌结直肠癌中的表达(77.5%, 62/80)低于高-中分化(93.4%,590/632;P=0.019);在直肠癌中的表达水平(96.8%,361/373)明显高于结肠癌(85.8%,291/339;P<0.01)。单因素和多因素分析显示,NLK的表达与结直肠癌患者总体生存率和无瘤生存率呈负相关(P<0.01),是结直肠癌患者的独立预测因素。NLK阳性表达率在21例转移性结直肠癌的正常黏膜、转移癌组织和原发癌组织中的表达率分别为57.1% (12/21)、76.2%(16/21)和81.0%(17/21),后两者比较,差异无统计学意义(P=0.851);而前者与原发癌比较,差异有统计学意义(P=0.010)。结论NLK在结直肠癌中可能发挥促癌作用,且具有预后判断的价值。
三黄归苍油纱外敷治疗老年性压力性溃疡

压疮又称压力性溃疡,是由于局部组织长期受压,发生持续缺血、缺氧、营养不良而致组织溃烂坏死的一类疾病,多见于截瘫、慢性消耗性疾患、大面积烧伤及深度昏迷等长期卧床患者。压疮是临床常见的并发症,一旦发生给病人带来极大痛苦,加重原发病情,增加病人及家属负担。重症或患多种疾病的患者在采取预防措施后仍有可能发生压疮,且经常较难治。因此如何提高老年性压疮的疗效和缩短治疗时限极为重要。我院采用三黄归苍油纱外敷治疗压疮,效果显著。
顺铂对人胃癌SGC7901细胞株中干细胞的影响

肿瘤组织中存在极少量具有无限自我更新能力和多向增殖潜能的细胞,即肿瘤干细胞(CSC),它们不仅在肿瘤的形成、发展和转移方面起着重要的作用,也可能是肿瘤耐药及抵抗放化疗的根源。Nanog基因作为全能性或多能性干细胞标记物,在胚胎干细胞和包括肺癌、胃癌在内的多种CSC中表达。本研究旨在揭示顺铂干预人胃癌SGC7901细胞后,对胃癌干细胞的影响。
芹菜素通过下调MDR-1/P-gp表达逆转结肠癌耐药细胞HCT-8/5FU多药耐药性的作用及机制

目的探讨芹菜素逆转结肠癌耐药细胞HCT-8/5FU多药耐药性作用及其机制。方法运用免疫细胞化学技术和逆转录多聚酶链式反应(RT-PCR)测定耐药蛋白P-糖蛋白(P-gp)及耐药基因MDR-lmRNA的表达情况。CCK-8法测定芹菜素对耐药细胞耐药逆转作用;荧光显微镜观察芹菜素、5-FU及二者联合作用时耐药细胞凋亡情况。结果芹菜素能够降低耐药蛋白P-gp糖蛋白及耐药基因MDR-1 mRNA的表达,同时能够逆转耐药细胞的多药耐药性,诱导更多细胞凋亡。结论菜素能够通过下调MDR-1/P-gp表达逆转HCT-8/5FU多药耐药性,从而有可能成为临床多药耐药逆转剂。
老年甲状腺癌超声影像与肿瘤血管内皮生长因子表达的相关性

目的探讨老年甲状腺癌的彩色多普勒血流特点与肿瘤血管内皮生长因子(VEGF)的相关性。方法术前采用多普勒超声记录84例老年甲状腺肿瘤患者的肿块类型及血流分级,并计算血流阻力指数(RI),术后采用免疫组化法检测肿瘤组织VEGF的表达情况。结果随着血流信号分级的增高,VEGF阳性表达率显著增多(χ~2=5.472,P<0.05)。VEGF在滤泡状癌组织中的阳性表达率最高(92.86%),显著高于甲状腺腺瘤(21.74%)和乳头状癌组织(55.32%)(P<0.01,P<0.05),且甲状腺腺瘤和乳头状癌组织中VEGF阳性表达率差异有统计学意义(P<0.05)。VEGF阳性表达组与阴性表达组RI值分别为(0.72+0.07)和(0.61±0.06),两组比较差异有统计学意义(P<0.05);且经Spearman相关分析发现,RI与VEGF阳性表达呈显著正相关(r=0.732,P<0.05)。结论多普勒彩超检测血流分级对老年甲状腺癌具有较高的诊断价值,而血流分级和RI能反映甲状腺癌VEGF表达情况。
血清HBV-DNA载量、乙肝免疫学标志物定量与老年慢性乙肝患者肝功能的关系

目的研究血清乙肝病毒(HBV)-DNA载量、乙肝免疫学标志物(HBV-M)定量与老年慢性乙肝患者肝功能的关系。方法对100例HBsAg阳性者血清用荧光定量多聚酶链式反应(FQ-PCR)定量测定HBV-DNA含量,用酶联免疫吸附试验(ELISA)测定HBV-M,同时进行肝功能谷丙转氨酶(ALT)、谷草转氨酶(AST)及前白蛋白(PA)测定。结果HBV-DNA定量测定中大三阳组显著高于小三阳组与HBsAg+HBcAb阳性组(t=4.55,P<0.05;t=3.83,P<0.05),小三阳与HBsAg+HBcAb阳性组之间差异无统计学意义(t=1.03,P>0.05)。HBV-DNA定量与ALT与AST含量呈一定的正相关(r=0.40,P<0.05;r=0.328,P<0.05),而与PA无相关性(r=0.03,P>0.05)。患者HBV-DNA的定量测定于各年龄组间不存在统计学差异(P>0.05)。结论患者HBV-DNA的定量分析同乙肝免疫学标志物关系密切,全面检测各项指标有利于老年乙肝患者筛检、诊断及治疗。
先天性巨大左心耳致心律失常影像表现一例

先天性巨大左心耳常引起心房颤动、心房扑动或阵发性室上速等房性心律失常。巨大左心耳造成了左心房体积增大及心肌细胞肥大,可能是引起异位或折返性激动的原因。本例术前频发心房扑动,术中切除左心耳,术后心电图恢复正常,病理结果亦证实左心耳心肌肥大变性,提示左心耳异常扩大可能是导致心律失常的原因。
浇钢工石棉致肺癌二例

石棉所致肺癌、间皮瘤是职业性肿瘤的一种,根据卫计委职业病防治工作情况的通报,全国30个省、自治区、直辖市和新疆生产建设兵团报告(不包括西藏、港、澳、台地区)统计报告,2007年共报告职业性肿瘤48例,石棉所致肺癌、间皮瘤4例,占8.33%。2009年共报告职业性肿瘤63例,石棉所致肺癌、间皮瘤11例,占17.46%。2010年共报告职业性肿瘤80例,石棉所致肺癌和间皮瘤共10例,占12.5%。2011年共报告职业性肿瘤92例,其中石棉所致肺癌和间皮瘤共8例,占8.70%。2012年共报告各类职业性肿瘤95例,其中石棉致肺癌、间皮瘤19例,占20%。现将2例石棉致肺癌病例报告如下。
黔北低海拔稻作区杂交水稻新品种引种初试

赤水属中亚热带湿润季风气候区,冬暖春早,夏季炎热多伏旱,全年日照少,初夏晚秋多阴雨,具有立体气候和地区差异显著的气候特点;年平均气温18 ℃(最高气温41 ℃,最低气温-2 ℃),年降雨量平均为1 268.8 mm,年均相对湿度82%,日照时数1 297.7 h,无霜期340~350 d,并随海拔高度上升而递减,800 m以下地区无霜期300 d左右,800 m以上地区无霜期210~300 d。
盐胁迫对6种禾本科牧草种子萌发及幼苗生长的影响

研究了NaCl胁迫对6种禾本科牧草种子发芽及幼苗生长的影响。结果表明:6种豆科牧草种子的相对发芽率均随着IaCI浓度的增大而降低,6种牧草幼苗的根长和苗长均随着IaCI浓度的增大而受到抑制,其抑制程度因草种而异。总体来看,黑麦草的耐盐性最好,苇状羊茅的耐盐性最差。
种植密度对不同基因型玉米杂交种生理特性及产量的影响

研究了不同种植密度对玉米品种先玉335、郑单958和良玉66生理特性和产量的影响。结果表明,随着种植密度增加,叶绿素相对含量降低,叶面积指数(LAI)增大,群体干物质积累量呈现升高趋势,但单株干物质积累则呈现降低趋势;产量呈现先增后降趋势。玉米群体和个体的产量性状相对较为协调的种植密度为:先玉335、良玉66为7.5万株/hm~2,郑单958为6万株/hm~2。
成人左侧肺静脉闭锁一例

单侧肺静脉闭锁(UPVA)系胚胎发育3个月时,在与原始肺静脉相连接的体静脉退化消失之后出现肺静脉或共同腔闭锁,继而出现单侧肺静脉血既不能引入体静脉系统,也不能汇入左心房的罕见先天性畸形。该病临床表现缺乏特异性,多在出生后或婴儿期因单侧肺部反复感染、咯血等诊断,部分患者可无明显症状直至成年才被发现。较典型的超声心动图特点是左心房患侧缘光滑,肺静脉开口缺失,同时伴有患侧肺动脉发育细小及对侧肺动脉代偿性增宽。多排螺旋CT和心血管造影除可用于UPVA确诊外,还可发现患侧弥漫性肺间质增厚以及纵隔内气管、支气管旁支气管静脉和肺静脉旁路开放,奇静脉扩张等表现。
An Investigation of the Formation of the Heat Wave in Southern China in Summer 2013 and the Relevant Abnormal Subtropical High Activities

In the summer of 2013, an unprecedented heat wave was experienced over a vast area of southern China. The great areal extent, duration, and strength of this high temperature are very rare. For the 2013 hot spell, the major and direct influence mostly came from the anomaly of the western Pacific subtropical high (WPSH). The abnormally strong and stable WPSH was associated with specific surrounding circulations. The eastward extension of a stronger Qinghai-Xizang high favored the westward extension of the WPSH. The weaker cold air activity from the polar region led to the northward shift of the WPSH and helped it to remain stable. In the tropics, the western segment of the ITCZ was abnormally strong in the period, and supported the maintenance of the WPSH from the south. In addition, the interdecadal variation of the WPSH provided a decadal background for the anomaly variation of the WPSH that summer.
Characterization of Stratospheric Aerosol Distributions during the  Volcanically Quiescent Period of 1998-2004

The Stratospheric Aerosol and Gas Experiment (SAGE) II aerosol extinction profiles at 1020 nm were used to study the distribution characteristics of stratospheric aerosols during the volcanically quiescent period of 1998-2004. The stratospheric aerosol distributions exhibited hemispheric asymmetry between the Northern Hemisphere (NH) and the Southern Hemisphere (SH). In the lower stratosphere below 20 km, the zonal averaged aerosol optical depths in the NH were higher than those of the corresponding SH; whereas at higher altitudes above 20 km, the optical depths in the SH-except the equatorial region—were higher than those of the NH. At 0-10°N and 10-20°N， the stratospheric aerosol optical depth (SAOD) exhibited larger values in boreal winter and lower values in the spring and summer; at 0-10°S and 10-20°S， the SAOD presented small seasonal variations. At 30—40°N， the SAOD presented larger values in the boreal fall and winter and lower values in the spring and summer; while at 30-40°S, the SAOD exhibited larger values in the austral winter and early spring and lower values in the summer and fall. These characteristics can mainly be attributed to the seasonal cycle of the dynamic transport, and the effects of the buildup and breakdown of the polar vortex. At 50-60°S, the SAOD exhibited extremely high values during austral winter associated with the Antarctic polar vortex boundary; at 50-60°N, the SAOD also exhibited larger values during the boreal winter, but it was much less obvious than that of its southern counterpart.
The Soil Moisture and Net Primary Production Affected by CO_2 and Climate Change Using a Coupled Model

In this paper, a coupled model was used to estimate the responses of soil moisture and net primary production of vegetation (NPP) to increasing atmospheric CO_2 concentration and climate change. The analysis uses three experiments simulated by the second-generation Earth System Model (CanESM2) of the Canadian Centre for Climate Modelling and Analysis (CCCma), which are part of the phase 5 of the Coupled Model Intercomparison Project (CMIP5). The authors focus on the magnitude and evolution of responses in soil moisture and NPP using simulations modeled by CanESM, in which the individual effects of increasing CO_2 concentration and climate change and their combined effect are separately accounted for. When considering only the single effect of climate change, the soil moisture and NPP have a linear trend of 0.03 kg m~(-2) yr~(-1) and -0.14 gC m~(-2) yr~(-2), respectively. However, such a reduction in the global NPP results from the decrease of NPP at lower latitudes and in the Southern Hemisphere, although increased NPP has been shown in high northern latitudes. The largest negative trend is located in the Amazon basin at -1.79 gC m~(-2) yr~(-2). For the individual effect of increasing CO_2 concentration, both soil moisture and NPP show increases, with an elevated linear trend of 0.02 kg m~(-2)yr~(-1) and 0.84 gC m~(-2) yr~(-2), respectively. Most regions show an increasing NPP, except Alaska. For the combined effect of increasing atmospheric CO_2 and climate change, the increased soil moisture and NPP exhibit a linear trend of 0.04 kg m~(-2) yr~(-1) and 0.83 gC m~(-2) yr~(-2) at a global scale. In the Amazon basin, the higher reduction in soil moisture is illustrated by the model, with a linear trend of -0.39 kg m~(-2) yr~(-1), for the combined effect. Such a change in soil moisture is caused by a weakened Walker circulation simulated by this coupled model, compared with the single effect of increasing CO_2 concentration (experiment M2), and a consequence of the reduction in NPP is also shown in this area, with a linear trend of -0.16 gC m~(-2) yr~(-2).
Diffusional kurtosis imaging: a promising technique for detecting microstructural changes in neural development and regeneration

Brain development is one of the most fascinating subjects in the field of biological sciences. Nonetheless, our scientific community still faces challenges in trying to understand the concepts that define the underlying mechanisms of neural tissue development. After all, it is a very complex subject to grasp and many of the processes that take place during central nervous system maturation are yet to be ascertained. Despite this challenge, we have come to recognize that understanding the natural course of normal brain tissue development on both microscopic and macroscopic scales is the key to deciphering the mechanisms through which these neural networks also heal and regenerate.
3D topographic correction of the BSR heat flow and detection of focused fluid flow

The bottom-simulating reflector (BSR) is a seismic indicator of the bottom of a gas hydrate stability zone. Its depth can be used to calculate the seafloor surface heat flow. The calculated BSR heat flow variations include disturbances from two important factors: (1) seafloor topography, which focuses the heat flow over regions of concave topography and defocuses it over regions of convex topography, and (2) the focused warm fluid flow within the accretionary prism coming from depths deeper than BSR. The focused fluid flow can be detected if the contribution of the topography to the BSR heat flow is removed. However, the analytical equation cannot solve the topographic effect at complex seafloor regions. We prove that 3D finite element method can model the topographic effect on the regional background heat flow with high accuracy, which can then be used to correct the topographic effect and obtain the BSR heat flow under the condition of perfectly flat topography. By comparing the corrected BSR heat flow with the regional background heat flow, focused fluid flow regions can be detected that are originally too small and cannot be detected using present-day equipment. This method was successfully applied to the midslope region of northern Cascadia subducting margin. The results suggest that the Cucumber Ridge and its neighboring area are positive heat flow anomalies, about 10%-20% higher than the background heat flow after 3D topographic correction. Moreover, the seismic imaging associated the positive heat flow anomaly areas with seabed fracture-cavity systems. This suggests flow of warm gas-carrying fluids along these high-permeability pathways, which could result in higher gas hydrate concentrations.
炎症性肠病的药物治疗

炎症性肠病的药物治疗石力夫（第二军医大学长海医院药学部，上海200433）炎症性肠病(inflammatory bowel diseases，IBD)是一种特殊的慢性肠道炎症性疾病，主要包括克罗恩病和溃疡性结肠炎。克罗恩病可发生于消化道任何部位，为慢性、反复发作的肠壁全层性炎症，常见于回肠末端和结肠。溃疡性结肠炎为发生于结肠的弥漫性、浅表性、局限于黏膜层的炎症，常见于直肠和乙状结肠。临床上，患者会表现为反复的腹痛、腹泻、黏液血便，甚至出现各种全身并发症，如视物模糊、关节疼痛、皮疹等。炎症性肠病病因目前尚不清楚。发病机制是在病原体、毒素、某些药物等抗原引发物的启动下，激活消化道局部免疫细胞和体液免疫反应，加上内毒素、一氧化氮和氧自由基的作用，导致组织黏膜损伤。
痰热清注射液致1例过敏性休克

痰热清注射液是我国研制的二类新药,主要由黄芩、熊胆粉、山羊角、金银花、连翘五味中药提取精制而成。主要功效为清热、解毒、化痰,可用于病属痰热阻肺型的急性支气管炎、急性肺炎(早期)。实验研究亦证明,其对呼吸道致病菌、肺炎链球菌、乙型溶血性链球菌、金黄色葡萄球菌、嗜血流感杆菌等有一定抑制作用。亦可用于其他组织感染,如胆囊炎、胰腺炎等。
清热除湿、凉血解毒法治疗癣菌疹体会

介绍运用赵炳南教授清热除湿、凉血解毒法及其系列方药治疗(足)癣菌疹体会。首先采用综合方法控制渗出,包括口服清热除湿类中药,外用苍肤洗剂、祛毒汤泡洗、甘草油调敷祛湿散、疱病清疮等;其次运用凉血解毒中药消除红斑,运用健脾利湿方药消除水肿,口服清热除湿汤控制过敏等;最后还要注意预防复发。
阴离子多聚物类杀微生物剂的研究进展

目的：概括阴离子多聚物作为Ⅰ型人类免疫缺陷病毒(HIV-1)感染抑制剂的兴衰历程，为未来更合理使用此类化合物以及杀微生物剂的研发提供参考。方法：查阅国内外文献，进行全面综合、整理和归纳，对阴离子多聚物类化合物作为抗病毒药物的应用进行综述。结果：阴离子多聚物类候选杀微生物剂临床失败的原因有：受试者对药物的依从性差；阴离子多聚物抑制R5型的HIV-1效果差，而R5型的HIV-1是性传播的最主要的型别；低浓度的阴离子多聚物能增加HIV-1感染的几率；阴离子多聚物能破坏阴道上皮细胞和阴道黏膜的保护作用等。这些分析都集中于药物本身，但临床前试验结果与临床结果之间存在较大的差距，预示着临床前的试验还有某些方面亟待完善，其中药物的安全性、有效性、药物在体内的停留时间及浓度、精液的活动等是研究热点。研究表明，阴离子多聚物能与精液来源的病毒增强子结合，并促进淀粉样纤维的形成，这有可能是阴离子多聚物临床失败的原因之一。结论：杀微生物剂的临床前研究尚有一些亟待完善的地方，未来开发阴离子多聚物类化合物需要谨慎对待，并从中吸取经验教训。
Cooperative Software Testing and Analysis: Advances and Challenges

In recent years, to maximize the value of software testing and analysis, we have proposed the methodology of cooperative software testing and analysis (in short as cooperative testing and analysis) to enable testing and analysis tools to cooperate with their users (in the form of tool-human cooperation), and enable one tool to cooperate with another tool (in the form of tool-tool cooperation). Such cooperations are motivated by the observation that a tool is typically not powerful enough to address complications in testing or analysis of complex real-world software, and the tool user or another tool may be able to help out some problems faced by the tool. To enable tool-human or tool-tool cooperation, effective mechanisms need to be developed 1) for a tool to communicate problems faced by the tool to the tool user or another tool, and 2) for the tool user or another tool to assist the tool to address the problems. Such methodology of cooperative testing and analysis forms a new research frontier on synergistic cooperations between humans and tools along with cooperations between tools and tools. This article presents recent example advances and challenges on cooperative testing and analysis.
A Survey on Silicon PUFs and Recent Advances in Ring Oscillator PUFs

Silicon physical unclonable function (PUF) is a popular hardware security primitive that exploits the intrinsic variation of IC manufacturing process to generate chip-unique information for various security related applications. For example, the PUF information can be used as a chip identifier, a secret key, the seed for a random number generator, or the response to a given challenge. Due to the unpredictability and irreplicability of IC manufacturing variation, silicon PUF has emerged as a promising hardware security primitive and gained a lot of attention over the past few years. In this article, we first give a survey on the current state-of-the-art of silicon PUFs, then analyze known attacks to PUFs and the countermeasures. After that we discuss PUF-based applications, highlight some recent research advances in ring oscillator PUFs, and conclude with some challenges and opportunities in PUF research and applications.
胸部恶性肿瘤放射治疗中诱发急性心肌梗死2例

放疗诱发的心脏病的影响因素包括总的放射计量和单次放射计量过大，尤其是放射总计量>35 Gy；放疗同时应用致心脏毒性的化疗药物；放射治疗的部位；存在冠心病危险因素，尤其是高血压及吸烟等。具体机制并不十分明确，主要与放疗导致的血管损伤及内皮功能障碍有关，黏附分子及von Willebrand因子的参与是主要的发病机制，放疗促进黏附分子诱导单核粒细胞黏附及迁移至内皮下，被吞噬细胞吞噬，脂质转运，逐渐形成脂肪条纹及斑块，加速动脉粥样硬化的形成，同时内皮细胞释放von Willebrand因子，其可减少血栓调节蛋白的释放，造成体内高凝状态，在放射治疗的诱因下，可导致动脉粥样硬化斑块破裂，诱发急性心肌梗死。放疗诱发的急性心肌梗死多见于前降支及右冠脉，治疗与常规急性心肌梗死治疗相同，应用抗凝，抗血小板聚集，调脂，抑制心肌重构等药物治疗，必要时可行经皮冠状动脉成形术甚至冠状动脉旁路移植术。
Binary metal-metal oxide superlattice nanostructures from their metal and metal oxide nanocrystals

In summary, the fabrication of binary superlattices with novel metal-metal oxide structures not only gives efficient catalysts for target reactions, but also gives better understanding of the strong metal support interaction effect and would allow the establishing of structure-performance relationships. Nevertheless, some problems still remain in the preparation and potential application. First, the interaction mechanism of surfactant with the monodispersed nanoparticles and the assembly pathway of superlattices with the desired structure is still not known. Although significant progress have been made in the synthesis of metal and metal oxide nanocrystals with the requisite size and morphology in the presence of organic surfactants to stabilize the surface [4,8], the optimization of reaction conditions, especially the selection of surfactants, is still empirical and the formation pathway is not always understood. This would surely influence their mutual interaction and subsequent self-assembly to superlattices. Meanwhile, the effective removal of the surfactant under mild conditions while maintaining the nanostructure is also highly desired. The other concern is the morphology effect of the superlattices. To date, the building blocks for metal-oxide systems are typically spherical nanoparticles, and less progress have been made with anisotropic morphologies with the preferential exposure of reactive facets. Morphology changes in the metal and metal oxides nanoparticles may alter the spatial arrangement of the superlattices, e.g., the selective deposition of metal nanoparticles on the facets of oxides, and the type and degree of metal-oxide interactions would affect catalytic behavior due to the well-known electronic and geometric effects.
Sperm retrieval rates and ICSI outcomes for men with nonobstructive azoospermia and the health of resulting offspring

In the article published in the Asian Journal of Andrology, Esteves et al.1 report on the results of a reasonably large (365) retrospective series of patients with nonobstructive azoospermia (NOA) who had sperm collected by microscopically assisted microdissection (microTESE) for intracytoplasmic sperm injection (ICSI). The results from NOA patients are compared with previously published data from a group of men with obstructive azoospermia collected during the same time frame. In addition, as is standard of care, the couples were offered ICSI with donor sperm if the microTESE procedure failed to yield sperm, providing a group from the same cohort who had ejaculated sperm used for ICSI.
Clinical features and an atypical WT1 mutant site in a child with incomplete Denys-Drash syndrome

Denys-Drash syndrome (DDS) is characterized by the triad of progressive nephropathy, urogenital malformation and Wilms' tumor. Incomplete variants present with nephropathy with either intersex disorders or Wilms' tumor.1 Most DDS patients carry WT1 mutations in exon 8 or 9.1 Here, we report an incomplete DDS child who carried a novel WT1 missense mutation in exon 6 and had unique clinical manifestations.
Cloud Neural Fuzzy PID Hybrid Integrated Algorithm of Flatness Control

In connection with the characteristics of multi-disturbance and nonlinearity of a system for flatness control in cold rolling process, a new intelligent PID control algorithm was proposed based on a cloud model, neural network and fuzzy integration. By indeterminacy artificial intelligence, the problem of fixing the membership functions of input variables and fuzzy rules was solved in an actual fuzzy system and the nonlinear mapping between variables was implemented by neural network. The algorithm has the adaptive learning ability of neural network and the indeterminacy of a cloud model in processing knowledge, which makes the fuzzy system have more persuasion in the process of knowledge inference, realizing the online adaptive regulation of PID parameters and avoiding the defects of the traditional PID controller. Simulation results show that the algorithm is simple, fast and robust with good control performance and application value.
左大腿黏液纤维肉瘤1例报道并文献复习

黏液纤维肉瘤(Myxofibrosarcoma,MFS)是一种由黏液细胞和黏液基质所形成的低度恶性肿瘤,极为罕见。现将赣南医学院第一附属医院骨科诊治的1例MFS的临床表现和组织病理学特征。
TGF-βl、Smad2、CyClinDl在胃癌中的表达及其临床意义

目的检测胃癌组织中TGF-β1、Smad2、CyclinDl蛋白表达,探讨三者与胃癌临床病理指标间的关系。方法收集承德医学院附属医院2007年1月至2012年1月具有完整临床及病理资料的胃癌手术切除标本60例(试验组),腔镜活检正常胃黏膜20例(对照组),应用免疫组织化学Elivision法检测这些组织中的TGF-β1、Smad2、CyclinDl蛋白表达情况。结果 TGF-β1、CyclinDl蛋白在胃癌中的表达明显高于正常胃黏膜组,而且胃癌中TGF-pi、CyclinDl蛋白的表达随胃癌的分化程度降低、淋巴结转移和临床分期升高而表达增加,Smad2蛋白在胃癌中的表达显著低于正常胃组织组,而且胃癌中Smad2蛋白的表达随分化程度降低、淋巴结转移及临床分期的升高而表达下降。结论通过对TGF-β1、Smad2和CyclinDl的指标检测诊断胃癌的临床分期,为探讨胃癌的发病机制的研究提供了新的思路.为TGF-β1、Smad2、CyclinDl成为靶向药物作用的潜在靶点提供一定的实验数据。
11例胰腺实性假乳头状瘤声像图特点及病理对照分析

目的探讨胰腺实性假乳头状瘤(SPTP)的超声表现与病理学关系。方法回顾性分析11例(12个瘤体)经手术及病理证实的实性假乳头状瘤的超声声像图特点,与病理学对照。结果 SPTP声像图分为囊性、囊实性、实性三种,囊性表现者发病率较低。当瘤体较大时,肿瘤出现囊实性改变较多,而当瘤体较小时,超声表现多以实性为主。囊实性表现为中低回声肿瘤的内部出现片状、裂隙状无回声为特点。结论 SPTP具有一定的声像学特点,超声检查可以准确显示肿瘤的部位和结构,为临床制定治疗策略提供客观依据。
Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains

VC2002, isolated from postweaning multisystemic wasting syndrome (PMWS)-affected pig, is a mixture of two porcine circovirus genotype 2b (PCV2b) viruses, K2 and K39. Preliminary experiments disclosed short-term adverse effects of K39, but not K2, on porcine foetuses. These findings led to the hypothesis that infection of immuno-incompetent foetuses with K2 confers a status of immunotolerance, and postnatal super-infection with K39 triggers PMWS. To explore this hypothesis, nine 55-day-old foetuses were inoculated in utero (three with K2-10~(4.3)TCID_(50), three with K39-10~(4.3) TCID_(50) and three with medium), and foeto-pathogenicity examined. At 21 days post-inoculation (dpi), K2 did not induce pathology, whereas pathological effects of K39 were evident. Twenty-four 45-day-old foetuses were subsequently inoculated to examine the long-term effect of K2, including six with K2-high dose-10~(4.3)TCID_(50), six with K2-low dose-10~(2.3)TCID_(50) and 12 mock-inoculated controls. Both doses resulted in five mummified foetuses and one live-born piglet each (69dpi). K2 was recovered from all mummies. K2 and K2-specific antibodies were not detected in serum of the two live-born piglets at birth, indicating full control of K2 infection. The K2-low dose-infected piglet was immunostimulated at day 2, but not the K2-high dose-infected piglet. Both non-stimulated and stimulated K2-infected piglets were super-inoculated with K39 at day 6 or 8 (taken as 0 days post super-inoculation). Low viral replication was observed in the non-stimulated K2-K39 piglet (up to 10~(3.3) TCID_(50)/g; identified as K39). In contrast, viral replication was extremely high in the stimulated K2-K39 piglet (up to 10~(5.6)TCID_(50)/g) and identified as K2, indicating that K2 infection is controlled during foetal life, but emerges after birth upon immunostimulation. However, none of the piglets showed any signs of PMWS.
Conservation of T cell epitopes between seasonal influenza viruses and the novel influenza A H7N9 virus

A novel avian influenza A (H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as H5N1 and H9N2. While there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved T-cell epitopes between endemic H1N1 and H3N2 influenza viruses and the novel H7N9 virus contributes to this observation. Here we demonstrate that a number of T cell epitopes are conserved between endemic H1N1 and H3N2 viruses and H7N9 virus. Most of these conserved epitopes are from viral internal proteins. The extent of conservation between endemic human seasonal influenza and avian influenza H7N9 was comparable to that with the highly pathogenic avian influenza H5N1. Thus, the ease of inter-species transmission of H7N9 viruses (compared with avian H5N1 viruses) cannot be attributed to the lack of conservation of such T cell epitopes. On the contrary, our findings predict significant T-cell based cross-reactions in the human population to the novel H7N9 virus. Our findings also have implications for H7N9 virus vaccine design.