mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Quantitative Survivability Analysis Using Probability Model Checking: A Study of Cluster-Based Vehicle Networks with Dual Cluster Heads

As an important part of future 5G wireless networks, a vehicular network demands safety, reliability and connectivity. In this context, networking survivability is usually considered an important metric to evaluate network performance. In this paper, we propose a survivability model for vehicle communication networking based on dual cluster heads, wherein a backup cluster head (CH) will be activated if the primary CH fails, thereby effectively enhancing the network lifetime. Additionally, we introduce a software rejuvenation strategy for the prime CH to further improve the survivability of the entire network. Using the Probabilistic Symbolic Model Checker (PRISM), we verify and discuss the proposed survivability model via numerical simulations. The results show that network survivability can be effectively improved by introducing an additional CH and further enhanced by adopting the software rejuvenation technique.
Cerebrospinal fluid neurogranin as a new player in prion disease diagnosis and prognosis

Neurogranin(Ng)and its role as Alzheimer’s disease(AD)biomarker:Ng is a calmodulin-binding protein mainly expressed in cerebral structures such as the cortex,hippocampus and striatum.It is mainly located in the dendritic processes,particularly in post-synaptic compartments,but also in the cytosolic compartment,being likely involved in the regulation of the intracellular calcium-calmodulin signaling pathway(Represa et al.,1990).In the last decade,a plethora of studies have demonstrated that cerebrospinal fluid(CSF)Ng is increased in AD patients and in individuals with an ADlike CSF profile(Kester et al.,2015a).This increase seems to be disease-specific because other neurodegenerative conditions including frontotemporal dementia,Lewy body dementia,Parkinson’s disease,progressive supranuclear palsy,multiple system atrophy or Huntington’s disease,present CSF Ng concentrations similar to controls(Wellington et al.,2016).Ng levels in CSF appear to be elevated in mild cognitive impairment(MCI)-affected individuals who progress to AD and are highly related to memory and cognitive function(Kester et al.,2015a;Tarawneh et al.,2016),which indicates that this protein may serve as an early AD biomarker with diagnostic utility in pre-dementia disease stages,and with prognostic utility to predict cognitive decline and MCI-to-AD conversion.
Transfer of mitochondria from mesenchymal stem cells derived from induced pluripotent stem cells attenuates hypoxia-ischemia-induced mitochondrial dysfunction in PC12 cells

Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury. Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology, the mechanisms are not fully understood. To address this issue, we first co-cultured 1.5 × 10~5 PC12 cells with mesenchymal stem cells that were derived from induced pluripotent stem cells at a ratio of 1:1, and then intervened with cobalt chloride(CoCl2)for 24 hours. Reactive oxygen species in PC12 cells was measured by Mito-sox. Mitochondrial membrane potential(ΔΨm)in PC12 cells was determined by JC-1 staining. Apoptosis of PC12 cells was detected by terminal deoxynucleotidal transferase-mediated dUTP nick end-labeling staining. Mitochondrial morphology in PC12 cells was examined by transmission electron microscopy. Transfer of mitochondria from the mesenchymal stem cells derived from induced pluripotent stem cells to damaged PC12 cells was measured by flow cytometry. Mesenchymal stem cells were induced from pluripotent stem cells by lentivirus infection containing green fluorescent protein in mitochondria. Then they were co-cultured with PC12 cells in Transwell chambers and treated with CoCl2 for 24 hours to detect adenosine triphosphate level in PC12 cells. CoCl2-induced PC12 cell damage was dose-dependent. Co-culture with mesenchymal stem cells significantly reduced apoptosis and restored ΔΨm in the injured PC12 cells under CoCl2 challenge. Co-culture with mesenchymal stem cells ameliorated mitochondrial swelling, the disappearance of cristae, and chromatin margination in the injured PC12 cells. After direct co-culture, mitochondrial transfer from the mesenchymal stem cells stem cells to PC12 cells was detected via formed tunneling nanotubes between these two types of cells. The transfer efficiency was greatly enhanced in the presence of CoCl2. More importantly, inhibition of tunneling nanotubes partially abrogated the beneficial effects of mesenchymal stem cells on CoCl2-induced PC12 cell injury. Mesenchymal stem cells reduced CoCl2-induced PC12 cell injury and these effects were in part due to efficacious mitochondrial transfer.
Bone marrow-derived mesenchymal stem cell transplantation attenuates overexpression of inflammatory mediators in rat brain after cardiopulmonary resuscitation

Emerging evidence suggests that bone marrow-derived mesenchymal stem cell transplantation improves neurological function after cardiac arrest and cardiopulmonary resuscitation;however,the precise mechanisms remain unclear.This study aimed to investigate the effect of bone marrow-derived mesenchymal stem cell treatment on expression profiles of multiple cytokines in the brain after cardiac arrest and cardiopulmonary resuscitation.Cardiac arrest was induced in rats by asphyxia and cardiopulmonary resuscitation was initiated 6 minutes after cardiac arrest.One hour after successful cardiopulmonary resuscitation,rats were injected with either phosphate-buffered saline(control)or 1 ×10~6 bone marrow-derived mesenchymal stem cells via the tail vein.Serum S100B levels were measured by enzyme-linked immunosorbent assay and neurological deficit scores were evaluated to assess brain damage at 3 days after cardiopulmonary resuscitation.Serum S100B levels were remarkably decreased and neurological deficit scores were obviously improved in the mesenchymal stem cell group compared with the phosphate-buffered saline group.Brains were isolated from the rats and expression levels of 90 proteins were determined using a RayBio Rat Antibody Array,to investigate the cytokine profiles.Brain levels of the inflammatory mediators tumor necrosis factor-α,interferon-γ,macrophage inflammatory protein-1α,macrophage inflammatory protein-2,macrophage inflammatory protein-3α,macrophage-derived chemokine,and matrix metalloproteinase-2 were decreased ≥ 1.5-fold,while levels of the anti-inflammatory factor interleukin-10 were increased ≥ 1.5-fold in the mesenchymal stem cell group compared with the control group.Donor mesenchymal stem cells were detected by immunofluorescence to determine their distribution in the damaged brain,and were primarily observed in the cerebral cortex.These results indicate that bone marrow-derived mesenchymal stem cell transplantation attenuates brain damage induced by cardiac arrest and cardiopulmonary resuscitation,possibly via regulation of inflammatory mediators.This experimental protocol was approved by the Institutional Animal Care and Use Committee of Fujian Medical University,China in January 2016(approval No.2016079).
Excitatory synapse impairment and mitochondrial dysfunction in Huntington's disease: heat shock factor 1 (HSF1) converging mechanisms

Heat shock factor 1(HSF1)is abnormally degraded in Huntington's disease(HD): HD is a neurodegenerative disorder characterized by severe cognitive and motor impairments. HD is caused by a CAG repeat expansion within exon 1 of the huntingtin(HTT)gene(The Huntington's Disease Collaborative Research Group, 1993). These expansions lead to the production of an aberrant mutant huntingtin protein(mHTT)that is prone to misfolding and aggregation. Expression and aggregation of mHTT is present in virtually all cell types in the body but preferentially affects medium spiny neurons of the striatum, a brain region that controls movement and some forms of cognition. Accumulation of mHTT leads to, but not only, transcriptional dysregulation, DNA damage, mitochondrial dysfunction and excitatory synaptic failure ultimately causing neuronal death. However, the molecular mechanisms by which mHTT exerts these defects are still unclear.
The mechanism and function of active DNA demethylation in plants

DNA methylation is a conserved and important epigenetic mark in both mammals and plants. DNA methylation can be dynamically established, maintained, and removed through different pathways. In plants, active DNA demethylation is initiated by the RELEASE OF SILENCING 1 (ROS1) family of bifunctional DNA glycosylases/lyases. Accumulating evidence suggests that DNA demethylation is important in many processes in plants. In this review, we summarize recent studies on the enzymes and regulatory factors that have been identified in the DNA demethylation pathway. We also review the functions of active DNA demethylation in plant development as well as biotic and abiotic stress responses. Finally, we highlight those aspects of DNA demethylation that require additional research.
Drought Stress Impairs Grain Yield and Quality of Rice Genotypes by Impaired Photosynthetic Attributes and K Nutrition

Drought is one of the most prevalent abiotic stresses that adversely affect rice productivity(Petrozza et al, 2014). Rice is very sensitive to drought stress and drought can cause 50% reduction in rice production globally(Yang et al, 2008). To meet the food needs for global population, 63% more agricultural production will be required by the year 2050 than the current production(Alexandratos and Bruinsma, 2012; FAO, 2017). Drought stress delays the time of flowering, leading to a reduced number of panicles, number of kernels and ultimately grain yield(Pantuwan et al, 2002; Iseki et al, 2014). The reproductive stage of rice is very sensitive to drought stress, and water limitation at this stage causes a serious reduction in rice yield(Aydinsakir et al, 2013) and biomass(Iseki et al, 2014). It is a major challenge to increase rice production under increasing drought due to changing climate, however, it may be achieved through use of drought-tolerant rice varieties having the ability to produce high yield under drought stress conditions(Luo, 2010).
Effect of Compound Laser Acupuncture-Moxibustion on Blood Glucose, Fasting Insulin and Blood Lipids Levels in Type 2 Diabetic Rats

Objective: To investigate the effect of compound laser acupuncture-moxibustion on blood glucose, fasting insulin and blood lipids levels in type 2 diabetes mellitus (T2DM) rats. Methods: Forty male Wistar rats were randomly divided into 4 groups, including the normal group, model control group, laser group and sham laser group (n=10 per group). The rats in the normal group were fed with a standard diet. Rats in other groups were fed with a high-sugar and high-fat diet for 4 weeks, then intraperitoneally injected with 1% streptozotocin to induce T2DM model. The laser group was irradiated by 10.6 μm and 650 nm compound laser on bilateral Pishu (BL 20), Shenshu (BL 23) and Sanyinjiao (SP 6) for 5 min, 6 times a week for 5 weeks. The sham laser group received the same treatment as the laser group, but without laser output. The model control group and normal group were not treated. Blood glucose levels were measured before and after 1, 2, 3, 4 and 5 weeks of treatment. The serum levels of fasting insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were analyzed after the last treatment. Results: The blood glucose levels in the model control group increased during the 5 weeks of treatment compared with the normal group (P<0.05), while those in the laser group were significantly lower than the model control group after weekly treatment (P<0.01 or P<0.05). After 1, 2 and 3 weeks of treatment, the blood glucose levels in the laser group decreased obviously compared with the sham laser group (P<0.01 or P<0.05). Compared with the normal group, the levels of fasting insulin, TC and LDL in the model control group notably increased (P<0.01 or P<0.05), while their levels in the laser group were significantly lower than the model control group after 5 weeks of treatment (P<0.05 or P<0.01). However, no statistically significant differences were observed in TG or HDL levels among the 4 groups (P>0.05). Conclusion: The compound laser acupuncture-moxibustion of 10.6 μm and 650 nm had positive effects on the regulation of hyperglycemia and insulin resistance in T2DM rats, which may be a potential treatment for T2DM, and also provide an alternative to the traditional acupuncture and moxibustion therapy.
Isosibiricin inhibits microglial activation by targeting the dopamine D1/D2 receptor-dependent NLRP3/caspase-1 inflammasome pathway

Microglia-mediated neuroinflammation is a crucial risk factor for neurological disorders. Recently, dopamine receptors have been found to be involved in multiple immunopathological processes and considered as valuable therapeutic targets for inflammationassociated neurologic diseases. In this study we investigated the anti-neuroinflammation effect of isosibiricin, a natural coumarin compound isolated from medicinal plant Murraya exotica. We showed that isosibiricin (10-50 μM) dose-dependently inhibited lipopolysaccharide (LPS)-induced BV-2 microglia activation, evidenced by the decreased expression of inflammatory mediators,including nitrite oxide (NO), tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) and interleukin-18 (IL-18). By using transcriptomics coupled with bioinformatics analysis, we revealed that isosibiricin treatment mainly affect dopamine receptor signalling pathway. We further demonstrated that isosibiricin upregulated the expression of dopamine D1/2 receptors in LPStreated BV-2 cells, resulting in inhibitory effect on nucleotide binding domain-like receptor protein 3 (NLRP3)/caspase-1 inflammasome pathway. Treatment with dopamine D1/2 receptor antagonists SCH 23390 (1 μM) or sultopride (1 μM) could reverse the inhibitory effects of isosibiricin on NLRP3 expression as well as the cleavages of caspase-1 and IL-1β. Collectively, this study demonstrates a promising therapeutic strategy for neuroinflammation by targeting dopamine D1/2 receptors.
Neural circuits and nicotinic acetylcholine receptors mediate the cholinergic regulation of midbrain dopaminergic neurons and nicotine dependence

Midbrain dopaminergic (DA) neurons are governed by an endogenous cholinergic system, originated in the mesopontine nuclei. Nicotine hijacks nicotinic acetylcholine receptors (nAChRs) and interferes with physiological function of the cholinergic system. In this review, we describe the anatomical organization of the cholinergic system and the key nAChR subtypes mediating cholinergic regulation of DA transmission and nicotine reward and dependence, in an effort to identify potential targets for smoking intervention. Cholinergic modulation of midbrain DA systems relies on topographic organization of mesopontine cholinergic projections, and activation of nAChRs in midbrain DA neurons. Previous studies have revealed that α4, α6, and β2 subunitcontaining nAChRs expressed in midbrain DA neurons and their terminals in the striatum regulate firings of midbrain DA neurons and activity-dependent dopamine release in the striatum. These nAChRs undergo modification upon chronic nicotine exposure. Clinical investigation has demonstrated that partial agonists of these receptors elevate the success rate of smoking cessation relative to placebo. However, further investigations are required to refine the drug targets to mitigate unpleasant side-effects.
Garcinol inhibits esophageal cancer metastasis by suppressing the p300 and TGF-β1 signaling pathways

Metastasis causes the main lethality in esophageal cancer patient. Garcinol, a natural compound extracted from Gambogic genera, is a histone acetyltransferase (HAT) inhibitor that has shown anticancer activities such as cell cycle arrest and apoptosis induction. In this study, we investigated the effects of garcinol on the metastasis of esophageal cancer in vitro and in vivo. We found that garcinol (5-15 μM) dose-dependently inhibited the migration and invasion of human esophageal cancer cell lines KYSE150 and KYSE450 in wound healing, transwell migration, and Matrigel invasion assays. Furthermore, garcinol treatment dose-dependently decreased the protein levels of p300/CBP (transcriptional cofactors and HATs) and p-Smad2/3 expression in the nucleus, thus impeding tumor cell proliferation and metastasis. Knockdown of p300 could inhibit cell metastasis, but CBP knockdown did not affect the cell mobility. It has been reported that TGF-β1 stimulated the phosphorylation of Smad2/3, which directly interact with p300/CBP in the nucleus, and upregulating HAT activity of p300. We showed that garcinol treatment dose-dependently suppressed TGF-β1-activated Smad and non-Smad pathway, inhibiting esophageal cancer cell metastasis. In a tail vein injection pulmonary metastasis mouse model, intraperitoneal administration of garcinol (20 mg/kg) or 5-FU (20 mg/kg) significantly decreased the number of lung tumor nodules and the expression levels of Ki-67, p300, and p-Smad2/3 in lung tissues. In conclusion, our study demonstrates that garcinol inhibits esophageal cancer metastasis in vitro and in vivo, which might be related to the suppression of p300 and TGF-β1 signaling pathways, suggesting the therapeutic potential of Garcinol for metastatic tumors.
Soil pressure and pore pressure for seismic design of tunnels revisited: considering water-saturated, poroelastic half-space

This paper describes a systematic study on the fundamental features of seismic soil pressure on underground tunnels, in terms of its magnitude and distribution, and further identifi es the dominant factors that signifi cantly infl uence the seismic soil pressure. A tunnel embedded in water-saturated poroelastic half-space is considered, with a large variety of model and excitation parameters. The primary features of both the total soil pressure and the pore pressure are investigated. Taking a circular tunnel as an example, the results are presented using a fi nite element-indirect boundary element (FE-IBE) method, which can account for dynamic soil-tunnel interaction and solid frame-pore water coupling. The effects of tunnel stiffness, tunnel buried depth and input motions on the seismic soil pressure and pore pressure are also examined. It is shown that the most crucial factors that dominate the magnitude and distribution of the soil pressure are the tunnel stiffness and dynamic soil-tunnel interaction. Moreover, the solid frame-pore water coupling has a prominent infl uence on the magnitude of the pore pressure. The fi ndings are benefi cial to obtain insight into the seismic soil pressure on underground tunnels, thus facilitating more accurate estimation of the seismic soil pressure.
原发性纵隔骨肉瘤一例

治疗主要通过手术完整切除肿瘤,化疗和放疗对于ESOS疗效目前还存在争议[15,17],而术式的选择需要根据肿瘤的大小、部位和累及的范围确定。ESOS患者的总体预后差,仅25%的患者术后可以获得较长生存期,而纵隔ESOS的预后极差,平均生存期仅4.0个月。本案例中,肿瘤已侵犯纵隔内的心脏大血管及右肺叶,手术治疗解决了患者上腔静脉梗阻症状。
先天性重度、极重度感音神经性聋患儿内耳及颅脑影像学评估

目的探讨先天性重度、极重度感音神经性聋患儿中内耳畸形及颅脑异常的发病情况,为人工耳蜗植入适应证的选择提供参考。方法对确诊为先天性重度、极重度感音神经性聋的245例患儿进行影像学检查,其中232例行颞骨高分辨率CT检查,204例行内听道MRI检查,196例行常规颅脑MRI检查,分析内耳结构异常及颅脑异常患儿的影像表现。结果行颞骨高分辨率CT扫描的232例中,195例内耳正常,37例内耳异常,占15.95%;行内听道MRI检查的204例中,167例正常,37例异常,占18.14%;行颅脑MRI平扫的196例中,110例正常,86例异常,占43.88%。耳蜗畸形包括:Michel畸形3例4耳,耳蜗未发育4例7耳,耳蜗发育不全2例3耳,不完全分隔Ⅰ型5例8耳,不完全分隔Ⅱ型5例10耳。前庭畸形16例26耳,半规管畸形14例24耳;内听道畸形7例12耳;前庭导水管畸形16例29耳。颅脑异常者包括:脑白质病61例,颅内发育变异9例,脑先天畸形7例,脑损伤、破坏性疾病6例,颅骨异常2例及松果体囊肿1例。结论本组先天性重度或极重度感音神经性聋患儿内耳畸形发生率为15.10%(37/245),颅脑异常发生率为43.88%(86/196),术前常规进行颞骨高分辨率CT、内听道MRI及颅脑MRI检查能够发现内耳及颅脑病变,可作为人工耳蜗植入适应人群的筛选及植入耳选择的参考。
Stabilizing High-voltage Cathode Materials for Next-generation Li-ion Batteries

The pressing demand for high-energy/power lithium-ion batteries requires the deployment of cathode ma-terials with higher capacity and output voltage. Despite more than ten years of research, high-voltage cathode mate- rials, such as high-voltage layered oxides, spinel LiNi_(0.5)Mn_(1.5)O_4, and high-voltage polyanionic compounds still can-not be commercially viable due to the instabilities of standard electrolytes, cathode materials, and cathode electrolyte interphases under high-voltage operation. This paper summarizes the recent advances in addressing the surface and interface issues haunting the application of high-voltage cathode materials. The understanding of the limitations and advantages of different modification protocols will direct the future endeavours on advancing high-energy/power lithium-ion batteries.
肠道微生态与儿童非酒精性脂肪性肝病

儿童非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)是年龄在18周岁以下的儿童及青少年肝脏慢性脂肪变性,5%以上肝细胞受累,但无过量饮酒史及其他导致肝脏慢性脂肪沉积等致病因素的临床综合征。其疾病谱包括非酒精性单纯性脂肪肝(non-alcoholic fatty liver, NAFL)、非酒精性脂肪性肝炎(non-alcoholic steatohepatitis, NASH)和及其相关的肝纤维化和肝硬化。NAFLD发病机制复杂,既往被广泛接受的“二次打击”学说认为,NAFLD发病是由胰岛素抵抗、高胰岛素血症引起肝细胞脂肪沉积,进而产生大量炎性细胞因子及脂肪因子,诱发线粒体功能障碍和氧化应激反应。但近年来逐渐被认同的“多次打击”理论认为,在“二次打击”的基础上,生活方式、肠黏膜屏障和肠道微生态、基因多态性等因素均对儿童NAFLD的发病起重要作用。目前儿童NAFLD无特效及规范治疗方案,以生活方式及饮食干预为主,可酌情辅以保肝、调节胰岛素抵抗及抗氧化应激治疗。随着对肠-肝轴认识的深入,肠道微生态与NAFLD的相关性逐渐被了解并深入研究,益生菌对NAFLD的可能治疗作用也受到关注。文章旨在对儿童NAFLD与肠道微生态相关性及益生菌对儿童NAFLD的治疗作用作一综述。
多价手足口病疫苗:现实与梦想

手足口病(hand,foot and mouth disease,HFMD)是一种常见于5岁以下婴幼儿和儿童的急性传染性疾病。过去的20年里,HFMD在世界范围内,特别是亚太地区频频暴发,少数患儿并发病毒性脑炎、脑干脑炎、肺水肿、急性弛缓性麻痹等重症甚至死亡。HFMD已成为亚太地区重要的公共卫生问题之一。
Heat Transfer and Thermal Characteristics Effects on Moving Plate Impinging from Cu-Water Nanofluid Jet

The present article is focused on modelling of flow and heat transfer behaviour of Cu-water nanofluid in a confined slot jet impingement on hot moving plate.Different parameters such as various moving plate velocities,nanoparticles at various concentrations,variation in turbulent Reynolds number and jet nozzle to plate distance have been considered to study the flow field and convective heat transfer performance of the system.Results of distribution of local and average Nusselt number and skin friction coefficients at the plate surface are shown to elucidate the heat transfer and fluid flow process.Qualitative analysis of both stream function and isotherm contours are carried out to perceive the flow pattern and heat transfer mechanism due to moving plate.The results revealed that average Nusselt number significantly rises with plate velocity in addition with jet inlet Reynolds number.Correlations of the average Nusselt numbers are presented.
Barrier effect of SiO_2 shell over hollow CeO_2/CuO@SiO_2 catalysts for broadening temperature window of total CO conversion

The hollow inverse CeO_2/CuO@SiO_2 catalysts with different Ce/Cu mass ratios were synthesized by the two-step hydrothermal and incipient wetness impregnation methods,and characterized by multi-technique characterizations,such as SEM,TEM,XRD,H2-TPR,XPS and N2 adsorption-desorption techniques.It is found that the hollow shell is composed of CuO and SiO_2,and CeO_2 nanoparticles are coated on the surface of CuO@SiO_2 support.And the CeO_2/CuO@SiO_2 catalyst with the Ce/Cu mass ratios of 1:1 denoted as 1CeO_2/CuO@SiO_2,which possesses a maximum amount of highly dispersed copper species and medium-sized CuO as well as the highest concentration of oxygen vacancies,exhibits the highest catalytic activity and widest full CO conversion window.The barrier effect of the SiO_2 shell effectively prevents the reduction of CuO species,which broadens temperature window of CO total conversion and enhances CO_2 selectivity above 155 °C over the 1CeO_2/CuO@SiO_2 catalyst in comparison with the CuO-CeO_2 and CeO_2-CuO catalysts.