mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Hypertonic and isotonic potassium solutions have different effects on vessel contractility resulting in differences in optimal resting tension in rat aorta

To compare high K~+-induced contraction and optimal resting tension measured by two commonly used techniques of hypertonic and isotonic K~+ in aortas with and without adventitial fat from various age rats. Methods: Three age groups of rats (15, 25, and 62 weeks) were used to prepare thoracic aortic rings in which adventitial fat was either removed or left intact. High K~+ (30 mmol/L)-induced contractions were observed under increasing resting tensions of 1.0, 1.5, 2.0, 2.5, 3.0, and 3.5 g. Optimal resting tension was the resting tension at which the aorta showed a maximal contraction. Results: The contractions induced by 2 kinds of high K~+ were significantly different. Hypertonic and isotonic K~+ induced a different style of contraction, and the pattern varied with different ages. At the age of 15 weeks, isotonic K~+-induced contractions were greater than hypertonic K~+-induced contractions. However, at the age of 62 weeks, isotonic K~+-induced contractions were smaller than hypertonic K~+-induced contractions. Optimal resting tensions measured by 2 kinds of high K~+ were inconsistent. Optimal resting tensions in different kinds of aortic preparations from various age rats were almost a constant of 2 g, determined by isotonic K~+, but a variable, determined by hypertonic K~+. The adventitial fat could delay the development of high K~+-induced contractions at different resting tensions, but had little effect on the maximal contractions. Conclusion: Hypertonic and isotonic K~+ may produce different contractions resulting in differences in optimal resting tension in rat aorta.
Dihydroartemisinin is an inhibitor of ovarian cancer cell growth

To investigate the anticancer activity of dihydroartemisinin (DHA), a derivative of antimalaria drug artemisinin in a panel of human ovarian cancer cell lines. Methods: Cell growth was determined by the MTT viability assay. Apoptosis and cell cycle progression were evaluated by a DNA fragmentation gel electro-phoresis, flow cytometry assay, and TUNEL assay; protein and mRNA expression were analyzed by Western blotting and RT-PCR assay. Results: Artemisinin and its derivjtives, including artesunate, arteether, artemether, arteannuin, and DHA, exhibit anticancer growth activities in human ovarian cancer cells. Among them, DHA is the most effective in inhibiting cell growth. Ovarian cancer cell lines are more sensitive (5-10-fold) to DHA treatment compared to normal ovarian cell lines. DHA at micromolar dose levels exhibits a dose- and time-dependent cytotoxicity in ovarian cancer cell lines. Furthermore, DHA induced apoptosis and G2 cell cycle arrest, accompanied by a decrease of Bcl-XL and Bcl-2 and an increase of Bax and Bad. Conclusion: The promising results show for the first time that DHA inhibits the growth of human ovarian cancer cells. The selective inhibition of ovarian cancer cell growth, apoptosis induction, and G2 arrest provide in vitro evidence for further studies of DHA as a possible anticancer drug in the clinical treatment of ovarian cancer.
Influence of omeprazole on pharmacokinetics of domperidone given as free base and maleate salt in healthy Chinese patients

To investigate the influence of omeprazole on the pharmacokinetics of domperidone given as free base and maleate salt. Methods: An open, randomized, 2-period crossover study with a washout period of 7 d was conducted in 10 healthy Chinese, male patients. In each study period, the patients were administered a single oral dose of 10 mg domperidone as free base or maleate salt on d 1, 20 mg omeprazole twice daily on d 2 and 3, and once on d 4. A single dose of 10 mg domperidone as free base or maleate salt was taken at 4 h after administration of omeprazole on d 4. Plasma samples were collected on d 1 and 4 after the administration of domperidone, and the plasma concentrations of domperidone were determined by a sensitive liquid chromatography-tandem mass spectrometry method. Results: For free-base domperidone, pretreatment with omeprazole resulted in a 16% decrease in maximum concentration (C_(max)), compared with administration alone (P < 0.05). However, for maleate salt, with the exception of an increase in t1/2, no pharmacokinetic parameters were significantly changed. When the free base and maleate salt were administered alone, no differences were found in any parameters between the 2 formulations. In contrast, when they were administered in the presence of omeprazole, the Cmax of domperidone given as free base was lower (25.9%) than that given as maleate salt (P < 0.05). Conclusion: Pretreatment of omeprazole does not affect the absorption of domperidone maleate, but leads to a moderately decreased rate of absorption of the free base.
Inhibitory effect of ginsenoside Rb1 on calcineurin signal pathway in cardiomyocyte hypertrophy induced by prostaglandin F2α1

To examine the antihypertrophic effect of ginsenoside Rb1 (Rb1) induced by prostaglandin F2α(PGF2α) in vitro and to investigate the possible mechanisms involved in the calcineurin (CaN) signal transduction pathway. Methods: The cardiomyocyte hypertrophy induced by PGF2α and the antihypertrophic effect of Rb1 were evaluated in primary culture by measuring the cell diameter, protein content, and atrial natriuretic peptide (ANP) mRNA expression. ANP and CaN mRNA expressions, CaN and its downstream effectors NFAT3 and GATA4 protein expressions, and the intracellular free Ca~(2+) concentration ([Ca~(2+)]i) were assayed by RT-PCR, Western blot, and fluorescent determination using Fura 2/AM, respectively. Results: PGF2α(100 nmol/L) significantly increased the cardiomyocyte diameter, protein content and [Ca~(2+)]i, and promoted ANP, CaN mRNA, and CaN/NFAT3/GATA4 protein expressions, which were inhibited by either Rb1 in a concentration-dependent manner (50,100, and 200 μg/mL) or L-arginine (1 mmol/L). NG-nitro-L-arginine-methyl ester, a nitric oxide synthase inhibitor, could abolish the effects of L-arginine, but failed to change the effects of Rb1 in the experiments above. Conclusion: The present data implicate that Rb1 attenuates cardiac hypertrophy, the underlying mechanism may be involved in the inhibition of the Ca~(2+)-CaN signal transduction pathway.
Modulation of Dishevelled and Vangl2 by All-trans-retinoic Acid in the Developing Mouse Central Nervous System and its Relationship to Teratogenesis

The response to exposure to all-trans-retinoic acid (RA) during embryogenesis varies from physiologic to severe teratogenic effects and is dependent upon the dose and the stage of development in all species. Vangl2 and Dishevelled genes play key roles in establishing planar cell polarity and regulating convergent extension movements during the neurula period. The effects of RA-mediated teratogenesis might be due to its misregulation of Vangl2 and Dishevelled genes. The aim of this study is to monitor the modulation of Vangl2 and Dishevelled in Kunming mouse embryos following maternal treatment with a single oral dose of 30 mg/(kg body weight) of RA during the neurula period. Exposure of 7.75 d embryos to RA induced characteristic morphological changes. The most obvious external effect was the failure of neural tube closure in the midbrain and forebrain regions in 10 d embryos, resulting in exencephaly in later embryos. RA treatment also led to a pronounced decrease of Vangl2 mRNA at 4 and 18 h and a pronounced increase at 66 h after maternal treatment, as detected by reverse transcription-polymerase chain reaction. Western blot analysis showed a marked decrease of Vangl2 protein at 18 and 42 h and a marked increase at 66 and 90 h after maternal treatment. Dishevelled 1/2/3 mRNA was significantly down-regulated at 4 and 18 h and up-regulated at 42 h in the fetus after RA treatment, except for an up-regulation of Dishevelled3 at 66 h. The Dishevelled2 mRNA and its protein matched each other. These results hinted that Vangl2 and Dishevelled genes might take part in RA teratogenesis of mouse embryos.
Bcl-XL Small Interfering RNA Sensitizes Cisplatin-resistant Human Lung Adenocarcinoma Cells

Bcl-XL is overexpressed in a variety of human tumors and is involved in tumorigenesis and chemoresistance. This study investigated the inhibitory effect of the hairpin Bcl-XL small interfering RNA (siRNA) on the expression of the Bcl-XL gene in the cisplatin (DDP)-resistant human lung adenocarcinoma cell line A549/DDP, and the effect of Bcl-XL siRNA on drug sensitization in A549/DDP cells. Bcl-XL siRNA and negative siRNA plasmids were constructed and stably transfected into A549/DDP cells. Reverse transcription-polymerase chain reaction and Western blot analysis were used to detect the target gene expression. Spontaneous apoptosis of cells was detected by acridine orange and ethidium bromide staining. Drug sensitivity of the cells to DDP was analyzed with dimethylthiazol-diphenyltetrazolium bromide (MTT) and flow cytometry. Expression levels of Bcl-XL mRNA and protein in siRNA stable transfectants were clearly reduced as compared with negative siRNA transfectants and untreated cells. MTT results indicated that Bcl-XL transfectants had a higher cell inhibition rate than the negative vector or untreated cells after treatment with 0.2–200 μg/ml DDP. Flow cytometry revealed increased apoptosis in Bcl-XL siRNA cells. After the addition of 20 μg/ml DDP, siRNA targeting of the Bcl-XL gene specifically down-regulated gene expression in A549/DDP cells, increased spontaneous apoptosis, and sensitized cells to DDP. The results showed that Bcl-XL siRNA contributed to an increase of DDP-induced cell death in non-small-cell lung cancer and sensitized cells to DDP, leading to increased the effectiveness of the drug in treating non-small-cell lung cancer.
Reversible Histone Acetylation Involved in Transcriptional Regulation of WT1 Gene

To validate the involvement of reversible histone acetylation in the transcriptional regulation of human Wilms' tumor 1 gene (WT1), we analyzed the roles of histone deacetylases (HDACs) and histone acetyltransferase in this epigenetic process. Of the six HDACs (HDAC1-6) examined, HDAC4 and HDAC5 were found to have significant repressing effects on the activity of the WT1 reporter gene, as revealed by luciferase reporter assays and quantitative real-time reverse transcription-polymerase chain reaction assays. Luciferase reporter assays showed that the histone acetyltransferase p300 was able to counteract the HDAC4/HDAC5-mediated repression and that p300/CBP synergized with transcription factors Sp1, c-Myb, and Ets-1 in activation of the WT1 reporter. Chromatin immunoprecipitation experiments showed that p300 promotes the acetylation level of histone H3 at the WT1 intronic enhancer. Based on these data, we proposed a hypothetical model for the involvement of reversible histone acetylation in transcriptional regulation of the WT1 gene. This study provides further insight into the mechanisms of transcriptional regulation of the WT1 gene and WT1-associated diseases treatment.
Modeling of the role of defects in sintered FeCrAlY foams

The metal sintering approach offers a cost- effective means for the mass-production of open-cell foams from a range of materials, including high-temperature steel alloys, which offer novel mechanical and acoustic properties. In a separate experimental study, the mechanical properties of open-celled steel alloy (FeCrAlY) foams have been characterized under uniaxial compression and shear loading. Compared to predictions from established models, a significant knockdown in material properties was observed. This knockdown was attributed to the presence of defects throughout the microstructure that result from the unique fabrication process. In the present paper, the microstructure of sintered FeCrAlY foams was modeled by using a finite element (FE) model. In particular, microstructural variations were introduced to a base lattice, and the effects on the strength and stiffness calculated. A range of defects identified under scanning electronic microscope (SEM) imaging were considered including broken ligaments, thickness variations, and pore blockages, which are the three primary imperfections observed in sintered foams. The corresponding levels of defect present in the material were subsequently input into the FE model, with the resulting predictions correlating well with experimental data.
Roles of F-box Proteins in Plant Hormone Responses

The F-box protein is an important component of the E3 ubiquitin ligase Skp1-Cullin-F-box protein complex. It binds specific substrates for ubiquitin-mediated proteolysis. The F-box proteins contain a signature F-box motif at their amino-terminus and some protein-protein interaction motifs at their carboxy-terminus, such as Trp-Asp repeats or leucine rich repeats. Many F-box proteins have been identified to be involved in plant hormone response as receptors or important medial components. These breakthrough findings shed light on our current understanding of the structure and function of the various F-box proteins, their related plant hormone signaling pathways, and their roles in regulating plant development.
Finite element simulations on the mechanical properties of MHS materials

Finite element simulations are carried out to examine the mechanical behavior of the metallic hollow sphere (MHS) material during their large plastic deformation and to estimate the energy absorbing capacity of these materials under uniaxial compression. A simplified model is proposed from experimental observations to describe the connection between the neighboring spheres, which greatly improves the computation efficiency. The effects of the governing physical and geometrical parameters are evaluated; whilst a special attention is paid to the plateau stress, which is directly related to the energy absorbing capacity. Finally, the empirical functions of the relative material density are proposed for the elastic modulus, yield strength and plateau stress for FCC packing arrangement of hollow spheres, showing a good agreement with the experimental results obtained in our previous study.
Design Research of a Type of Soft PLC Integrated Monitoring Function

It＇s a new direction for the industry control configuration software that integrates control strategy configuration module with monitoring configuration module. Firstly of all, this paper analyzes the technical characteristics of those arisen industrial control software, Then the structure of the new-type soft PLC and design method are proposed, and expatiates on the function structure of control strategy configuration module and monitoring configuration module and the data exchange technique between control strategy configuration module and animate picture monitoring configuration module. At last, we developed a new-type industrial control configuration software which integrates control strategy configuration function with monitoring configuration function in the light of the data exchange model.
Electronic Structures of Wurtzite GaN with Ga and N Vacancies

The electronic band structures of wurtzite GaN with Ga and N vacancy defects are investigated by means of the first-principles total energy calculations in the neutral charge state. Our results show that the band structures can be significantly modified by the Ga and N vacancies in the GaN samples. Generally, the width of the valence band is reduced and the band gap is enlarged. The defect-induced bands can be introduced in the band gap of GaN due to the Ga and N vacancies. Moreover, the GaN with high density of N vacancies becomes an indirect gap semiconductor. Three defect bands due to Ga vacancy defects are created within the band gap and near the top of the valence band. In contrast, the N vacancies introduce four defect bands within the band gap. One is in the vicinity of the top of the valence band, and the others are near the bottom of the conduction band. The physical origin of the defect bands and modification of the band structures due to the Ga and N vacancies are analysed in depth.
X-Ray Photoelectron Spectroscopy and Reflection High Energy Electron Diffraction of Epitaxial Growth SiC on Si(100) Using C_(60) and Si

The formation of silicon carbide upon deposition of C60 and Si on Si(100) surface at 850°C is studied via x-ray photoelectron spectroscopy and reflection high energy electron diffraction (RHEED). The C 1s, O 1s and Si 2p core-level spectra and the RHEED patterns indicate the formation of 3C–SiC.
Influences of Pressure and Substrate Temperature on Epitaxial Growth of γ-Mg_2SiO_4 Thin Films on Si Substrates

An epitaxial γ-Mg_2SiO_4 thin film can be a good buffer between the Si substrate and some oxide thin films. For high temperature superconducting multilayer structures, hopefully it can be taken as an insulating layer to replace the widely used MgO film. To explore such possibilities, we carry out systematic studies on the influences of pressure and substrate temperature on the epitaxy of γ-Mg_2SiO_4 thin films grown on Si (100) substrates using rf magnetron sputtering with an Mg target of purity of 99.95 percent. With the substrate temperature kept at 500 °C and the pressure changing from 10 Pa to 15 Pa, in the XRD spectra the γ-Mg_2SiO_4 (400) peak grows drastically while the MgO (200) peak is suppressed. Keeping the pressure at 15 Pa and increasing the temperature from 500°C to 570°C further can improve the film epitaxy, while working at 780°C and 11 Pa seems to give very good results. X-ray photoelectronic spectroscopy and  scan are used to characterize the stoichiometry, crystallinity, and in-plane growth of the samples.
Scattering-Enhanced Femtosecond Optical Parametric Generation

Scattering-enhanced femtosecond optical parametric generation (OPG) has been observed and studied in detail, which may decrease the pump threshold of OPGs dramatically and may make OPGs reliable. The experimental demonstration in a LiNbO_3 crystal shows that strong enough OPG can be obtained at pump intensity of only about 30 GW/cm~2 which is well below the crystal damage threshold. Either a synchronized pulsed laser or a cw laser has been used as the scattering source, of which the wavelength, spectral shape and irradiating angle are not strictly required.
Coherent and Incoherent Interactions between Discrete-Soliton Trains in Two-Dimensional Light-Induced Photonic Lattices

The coherent and incoherent interactions between discrete-soliton trains are numerically investigated in light-induced two-dimensional photonic lattices. The solutions of discrete-soliton trains for diamond and square lattices are obtained by Petviashvili iteration method. It is found that for both the kinds of lattices, two in-phase (out-of-phase) discrete-soliton trains attract (repel) each other, and the intermediates are always accompanied with energy transfer. While the interaction forces between two incoherent discrete-soliton trains are always attractive.
High-Power Operation of Uncoated Strain-Compensated Quantum Cascade Lasers at 4.8μm

High-power operation of uncoated 22-μm-wide quantum cascade lasers (QCLs) emitting at λ ≈ 4.8 μm is reported. The emitting region of the QCL structure consists of a 30-period strain-compensated In_(0.68) Ga_(0.32)As/In_(0.37) Al_(0.63) As superlattice. For a 4-mm-long laser in pulsed mode, a peak output power is achieved in excess of 2240mW per facet at 81K with a threshold current density of 0.64 kA/cm~2. The effects of varying the cavity lengths from 1 to 4 mm on the performances of the QCLs are analysed in detail and the low waveguide loss of only about 1.4 cm~(-1) is extracted.
Generation of Cluster States in Cavity QED

We propose two schemes for the generation of cluster states in the context of cavity quantum electrodynamics (QED). In the first scheme, we prepare multi-cavity cluster states with information encoded in the coherent states. The second scheme is to generate multi-atom cluster states, where qubits are represented by the states of cascade Rydberg atoms. Both the schemes are based on the atom-cavity interaction and the atomic spontaneous radiation can be efficiently reduced since the cavity frequency is largely detuned from the atomic transition frequency.
Trichostatin A在体内对鸡胚翅芽发生中基因表达模式的作用

表观遗传和染色质重塑参与了胚胎形成及肿瘤发生，乙酰化作用是调控基因转录的关键修饰之一，组蛋白去乙酰基酶抑制剂被视为肿瘤治疗中最有前景的药物开发目标．然而哪些基因在体内对乙酰化作用修饰更敏感？这些基本问题仍未完全知晓．以鸡胚翅芽作为实验模型，用HDAC抑制剂trichostafin A（TSA）处理翅芽，研究翅芽发生中一系列基因对TSA的反应．结果显示，TSA（75μmol／L）能够改变某些对翅芽发育具有重要功能的基因的表达水平，其中SF／HGF和Twist1基因的表达被上调；FGF8，Shh，TCF4，Myf5及MyoD基因的表达被下调；与此不同，Pax3，Paraxis，CREB和PCNA基因的表达水平不受TSA作用的影响．结果提示，发育的鸡胚翅芽能够作为一个便利的、供体内研究HDACs抑制剂对基因表达作用的模型，有助于提高对染色质重塑和表观遗传对基因表达的调控作用，以及对正在开发的抗癌药物的认识．
南海北部珊瑚共生虫黄藻密度的种间与空间差异及其对珊瑚礁白化的影响

与造礁石珊瑚共生的虫黄藻（Zooxanthellae）的密度和色素变化是珊瑚礁白化的最主要特征．通过对海南三亚鹿回头、小东海和广东省大亚湾海区共12科21属39种石珊瑚的共生体虫黄藻密度分析，得出：（i）与珊瑚共生的虫黄藻平均密度变化于0．67×10^6-8．48×10^6cells／cm。之间，具有明显的种间差异性，其中枝状珊瑚的共生体虫黄藻密度相对较低（0．67×10^6-2．47×10^6cells／cm^2），而块状珊瑚的共生体虫黄藻密度相对较高（1.00×10^6-8．48×10^6cells／cm^2）；（ii）水深4m以内珊瑚共生虫黄藻密度普遍比水深7m左右接近海底的珊瑚的高；（iii）相对高纬度的大亚湾（～22°N）与三亚（～18°N）相比，海温相同的环境下，与珊瑚共生的虫黄藻密度的纬度差异不明显；（iv）局部白化的同一珊瑚个体，未白化部分的虫黄藻密度〉半白化部分〉完全白化部分．进一步分析认为：（i）块状珊瑚与枝状珊瑚虫黄藻密度的差异性可能是导致枝状珊瑚易于白化的主要原因，如低密度共生虫黄藻导致枝状的鹿角、杯形珊瑚等易于白化死亡；（ii）珊瑚共生体虫黄藻密度受人为因素制约，如沉积物增加、潜水活动造成海水浑浊、海产品养殖活动引起的硝酸盐、磷酸盐增加等都可能导致共生虫黄藻密度降低．
激光光凝联合小梁切除术与睫状体冷冻术治疗新生血管性青光眼疗效观察

评价氪多波长激光视网膜广泛光凝或虹膜局部光凝联合小梁切除术治疗新生血管性青光睛与睫状冷冻术治疗的疗效比较。方法新生血管性青光眼64眼,其中32眼先用氪激光对视网膜广泛光凝或虹膜局部光凝后再进行小梁切除术另外32眼行睫状体冷冻治疗。结果术后（1～12）个月观察及随访,激光光凝联合小梁切除术32眼,28眼眼压〈21mmHg;睫状体冷冻术治疗的32眼,14眼（43.1%）眼压〈21mmHg,10眼术后降眼压药物治疗眼压仍不能控制,8眼眼球萎宿。结论激光光凝联合小梁切除术治疗新生血管性青光眼,疗效确定,较睫状体冷冻术治疗成功率高。
ILK治疗视网膜新生血管研究热点

整合素连接激酶（integrin-linked kinase,ILK）是一种最近发现的能够与整合素β1、β3亚基胞浆域结合的丝氨酸/苏氨酸蛋白激酶,它包含三个独特的结构域：N末端4个锚蛋白（ankyrin,ANK）重复序列,C末端激酶结构域,两者之间是磷脂酰肌醇结合结构域（phosphinositide binding motif）,简称PH结构域。ILK是PI-3K信号传导通路的重要成员,PI-3K产物PIP3与ILK的PH结构域结合后使之活化,进一步可以通过磷酸化下游底物PKB/AKT、GSK-3促进VEGF的表达;另一方面VEGF与相应的受体结合后也可以激活ILK,活化的ILK进一步通过调节细胞增殖,侵袭和凋亡过程参与调控VEGF诱导的新生血管形成。因此抑制ILK功能可能产生的这种双重作用效果——抑制VEGF的表达、抑制VEGF诱导的血管网形成——表明ILK很可能成为治疗视网膜新生血管的新思路。
胃黏膜保护剂的药理作用和应用评价

一、胃黏膜保护剂的概念和分类胃黏膜损伤因子包括内源性因子如高浓度胃酸、胃蛋白酶、幽门螺杆菌（Hp）、十二指肠液和外源性因子如非甾体类抗炎药（NSAID）、乙醇等。胃黏膜具有下列防御和修复机制，包括黏液-碳酸氢盐屏障、黏膜屏障、黏膜下血流量、细胞更新、前列腺素（prostaglandins，PG）和生长因子等。
以间歇性肠梗阻为特征的小肠类癌一例

患者女，61岁，因腹胀、间歇性腹痛伴体质量进行性下降5年，加重3个月于2006年11月29日入院。患者5年前无明显诱因下出现腹部不适、腹胀，进脂餐后症状明显，无恶心、呕吐。此后症状反复发作，2003年因腹痛、腹胀行B超检查提示胆囊有胆固醇结晶，给予消炎利胆药后症状无改善。此后患者体质量进行性减轻，至2005年下降15kg而再次入住他院，胃镜（-），B超示胆囊息肉，肠镜示结肠息肉并予套切，腹部平片及小肠造影无异常。出院后出现腹胀、腹痛，嗳气发作频繁，且进食后加剧，无纳差但惧食，肛门排气减少，大便3～7d解1次。并时常出现剧烈腹痛，为绞痛，阵发性加重，间隔时间不定，不用药物可自行缓解。近3个月腹痛进行性加重，伴明显嗳气，肛门排气明显减少，有恶心与呕吐，呕吐物为宿食，呕吐后腹痛无缓解，腹胀、腹痛明显时卧床不起，自行禁食数天后症状缓解，无腹痛时自觉体力与精神较好，可进行日常活动。
蚯蚓纤溶酶降低胃癌细胞与血管内皮细胞粘附性及CD44v6表达

目的研究蚯蚓纤溶酶对人胃癌细胞株MGC803的增殖抑制作用以及对胃癌细胞与血管内皮细胞粘附性和粘附分子CD44v6表达的影响。方法采用四甲基偶氮唑盐（MTT）法检测蚯蚓纤溶酶对人胃癌细胞株MGC803增殖的影响；体外粘附实验观察蚯蚓纤溶酶对胃癌细胞MGC803与血管内皮细胞ECV304粘附性的影响；流式细胞术观察蚯蚓纤溶酶作用于MGC803细胞后CD44v6蛋白表达的变化；RT—PCR法检测蚯蚓纤溶酶对MGC803细胞CD44v6mRNA表达的影响。结果蚯蚓纤溶酶各用药组（2、4、8uku／ml）对MGc803细胞均有抑制生长作用（P〈0．01），且在一定浓度范围内呈剂量依赖关系。蚯蚓纤溶酶4、8uku／ml作用于MGC803细胞48h后，与对照组相比，胃癌细胞与血管内皮细胞的粘附性明显下降（P〈0．01）。2、4、8uku／ml蚯蚓纤溶酶作用后，CD44v6蛋白表达水平呈剂量依赖性下降，平均荧光强度分别为84．80±2．14、30．69±3．66、21．75±2．25，与对照组相比，差异均有统计学意义（P〈0．05或0．01）。DNA半定量分析表明，各实验组CD44v6 mRNA表达也明显减少（P〈0．01）。结论蚯蚓纤溶酶有抑制胃癌细胞增殖的作用，并可降低胃癌细胞与血管内皮细胞的粘附性、降低胃癌细胞粘附分子CD44v6的表达，提示蚯蚓纤溶酶可能具有潜在抗肿瘤转移作用。
草酸铂腹腔化疗腹膜毒性反应的实验研究

腹腔化疗因其药代动力学优势，在腹腔转移癌治疗中起重要作用。草酸铂作为第三代铂类，临床应用日益广泛，但存在严重的急性腹膜炎样症状。我们以小鼠草酸铂腹腔灌注模型作为研究对象，探讨其腹膜毒性反应的成因，为临床预防和处理提供线索。
从吕仁和“三期”辨治糖尿病探讨中医认识疾病之动态观

随着中医对糖尿病认识的逐步深入，传统的“三消”辨证法已远远不足以应对糖尿病不同分型及各个阶段的证候特点，从而众多文献报道糖尿病中医从肾论治、从脾论治、从肝论治乃至从五脏论治，或从虚、从瘀、从毒论治等等。笔者也曾一度困惑于其中，不得其要，有幸从师于我国著名的中医学专家吕仁和教授于门诊诊病，而受益匪浅。吕老从事糖尿病及其并发症和肾脏病的中医临床.
温阳益心法与心肌缺血预适应“第二窗口”保护的实验研究

目的：研究温阳益心中药对心肌缺血再灌注（IR）损伤大鼠心肌的保护作用是否通过心肌缺血预适应（IP）保护机制而实现。方法：建立IR心肌梗死动物模型，采用NBT染色、称重法检测心肌梗死范围，光镜观察病理心肌损伤程度。结果：中药组、IP组与IR组心肌梗死范围、心肌损伤程度差异有显著性，中药组与IP组比较无显著性差异。结论：温阳益心中药和IP可以减少心肌梗死范围，减轻心肌损伤程度，且功效具有相近性，其保护作用可能为通过IP保护机制而实现。
夹脊穴与脊髓神经节段支配及其经络脏腑效应

夹脊穴是一组取穴简单、疗效高的穴位，被针灸临床普遍应用。通过对夹脊穴的临床应用及穴位的功能主治，探讨其与脊髓神经节断的关系和相关的规律性，进一步得出经穴主治的神经节段理论，对指导针灸临床取穴、配穴，并进一步探讨经穴的主治规律具有重要意义。
中药鼻敏康颗粒对变应性鼻炎C57BL6小鼠行为学及血清IgE的影响

8周龄雄性C57BL6小鼠40只，体重18g～22g，由中国医科大学实验动物中心提供。随机分为空白、模型、鼻敏康颗粒及氯雷他定对照4组，每组10只。
中药抗肿瘤血管生成疗法的现状及进展

肿瘤是严重危害人类健康的常见病和多发病，肿瘤防治一直都是亟待解决的医学难题。肿瘤的生长和转移取决于肿瘤血管的形成，因此可以通过阻断肿瘤新生血管的生长，使肿瘤得不到营养来遏制肿瘤生长和转移，并最终达到“饿死”肿瘤的目的。随着研究的深入，发现一些中药或其有效成分能抑制肿瘤血管生成，发挥抗肿瘤作用。本文就近几年来中药抗肿瘤血管生成的研究进展作一概述。