mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
LSM particle size effect on the overall performance of IT-SOFC

In this paper, we reported the fuel cell performance with La_(0.8)Sr_(0.2)MnO_3 (LSM)/Ce_(0.8)Sm_(0.2)O_(1.9) (SDC) composite cathode prepared from LSM powders of different particle sizes via the silk-printing technique. It was found that the change in particle size of LSM nanoparticle from 40 to 90 nm resulted in an increase in the maximum power density from 132 to 228 mW/cm~2 at 650 oC with H_2 as fuel and O_2 as oxidant. And the polarization resistance of the electrode decreased from 2.547 to 1.034 Ω·cm~2. Concerning the particle size of electrode materials, a higher activity was anticipated with smaller particles because a large number of TPB or electrode surface sites along with a higher porosity could be developed. However, this study showed that the electrode prepared with particles of larger diameter had fine and uniform microstructure resulting in higher power density and lower overpotential, where homogeneous distribution of particles and pores was beneficial for increasing the electrochemical active area and the electronic conductivity of the electrodes as well as the gas diffusion for the reactants.
Preparation and morphology of nano-size ceria by a stripping precipitation using oxalic acid as a precipitating agent

The precursors organic cerium deposits were obtained by a stripping precipitation method from cerium-loaded P507 organic phase using oxalic acid as a precipitating agent and nano-sized ceria particles were prepared by calcining the precursors at 500 °C. The morphologies, phase structure of the precursors and ceria particles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TG-DSC) and infrared spectroscopy analysis (FTIR). The results indicated that the concentration of oxalic acid had a little impact on the morphology of the precursors, which was blocky-shape Ce_2(C_2O_4)_3·10H_2O with a monoclinic lattice structure. With the volume ratio of organic phase and oxalic acid aqueous solution (aqueous phase) varied from 1:3 to 1:6, the morphologies of the precursors Ce_2(C_2O_4)_3·10H_2O precipitates gradually changed from inhomogeneous blocky-shape to uniform spherical particles. The precursors could be turned to CeO_2 crystal particles with a fluorite structure by calcining, and the products ceria particles were similar in the size and morphology to the precursors.
Two new β-carboline-type alkaloids from Stellaria dichotoma var. lanceolata

Two new β-carboline-type alkaloids, dichotomine K (1) and dichotomine L (2), were isolated from the roots of Chinese medicinal plant Stellaria dichotoma L. var. lanceolata Bge. Structures of 1 and 2 were determined on the basis of chemical and spectroscopic means.
Covalent functionalization of graphene oxide by 9-(4-aminophenyl)acridine and its derivatives

Graphene/acridine (G-Acr) hybrid structures were synthesized through covalent functionalization of graphene oxide with 9-(4- aminophenyl)acridine (APA) and its derivatives. The G-Acr hybrids were characterized by Fourier transform infrared spectroscopy, ultravioletCvisible spectrophotometry, thermal gravimetric analysis and Raman spectroscopy. X-ray photoelectron spectroscopy confirms that the binding energies of APA and its derivatives shifted to higher values, revealing pronounced charge transfer at the interface of graphene and organic molecules.
肝树突细胞肿瘤2例报道并文献复习

目的探讨肝滤泡树突细胞肉瘤(follicular dendritic cell sarcoma,FDCS)和指状突树突细胞肉瘤(interdigitating dendritic cell sarcoma,IDCS)的临床及病理组织学特征。方法采用免疫组化EnVision两步法及原位杂交法对1例肝FDCS和1例肝IDCS进行临床病理学分析,并复习相关文献。结果例1男性,46岁,肝FDCS患者,伴有肝炎及肝硬化;例2女性,64岁,肝IDCS患者,同时伴有乳腺浸润性小叶癌。2例患者均行肝肿物切除术。2例肿瘤细胞均呈梭形或卵圆形,异型明显,呈结节状、束状或旋涡状排列,可见核分裂象,背景富于淋巴细胞和浆细胞。其中IDCS部分区域可见纤维化分隔。二者均呈炎性假瘤样表现。免疫表型:肝FDCS表达CD21、CD35,肝IDCS表达S-100蛋白,二者均表达CD68、lysozyme、vimentin;FDCS背景以B淋巴细胞为主,而IDCS则以T细胞为主。二者EBER检测均呈(-)。例1术后行化疗,术后2个月复发,5个月后行栓塞术,病情平稳。例2合并分期为T_1CN_0M_0的乳腺浸润性小叶癌,术后随访2个月,健在。结论FDCS和IDCS均可发生于肝脏,极其少见,可发生于中老年人,形态学均可呈炎性假瘤样表现,免疫表型有助于诊断和鉴别诊断。肝IDCS预后差。EB病毒在肿瘤发病机制中的作用尚不清楚。
3-D NUMERICAL SIMULATIONS OF FLOW LOSS IN HELICAL CHANNEL

The flow loss of a helical channel Magnetohydrodynamic (MHD) thruster without MHD effect was numerically studied with 3-D simulations, and a flow loss coefficient ζ was defined to quantify the flow loss and its influencing factors were studied. The results show that ζ decreases in a first-order exponential manner with the pitch of a helical wall and the Reynolds number, and it declines slowly when t/T>0.2 and Re>10~5, a flow guide makes the flow more smooth and uniform, especially in the flow guide and helical wall sub-regions and thus reduces the flow loss greatly, by about 30% with the averaged value of ζ from 0.0385 to 0.027, a rectifier weakens the helical flow and strengthens the axial one in the rectifier and outlet sub-regions, thus reduces the rotational kinetic pressure with the averaged value of ζ declining about 4% from 0.0385 to 0.037, and ζ decreases with a rectifier’s axial length when Re >10~5 .
Au纳米阳极阻挡层对聚合物太阳能电池性能的影响

自Tang研制出光电转换效率为1%的有机太阳能电池后,近20年来,有机太阳能电池取得了可喜的进展,已经实现7.4%的光电转换效率.但是与无机太阳能电池相比,其转换效率较低,离实用化还有一定的距离,因此提高聚合物太阳能电池的光电转换效率是目前此类电池研究的一个重点.决定太阳能电池光电转换效率的主要参数有短路电流.开路电压和填充因子,而影响这几个参数的因素主要有:材料的光谱响应特性、材料的能级特性、材料的迁移率等,因而本文从新材料的使用、分子改造、新的成膜工艺和膜的形态结构的改进等方面提高器件的性能.材料的光谱响应一般对于材料能级结构的影响较大,一般能级和地面太阳辐射光谱匹配较好的材料有更好的表现.但大多数时候材料的响应光谱往往和太阳辐射光谱没有很好地匹配,因而使用不同的材料光谱的响应特性就会不同.
Time matters: Some interesting properties of the population differentiation measures GST and D overlooked in theequilibrium perspective

Genetic differentiation of populations has been traditionally quantified by Wright's F-statistics, typically assuming mutation-migration-drift equilibrium. However, the equilibrium perspective can be unrealistic as many natural populations are likely not yet in equilibrium. Therefore, understanding the behaviors, robustness, and power of the differentiation indexes under non-equilibrium conditions has important implications. Here, we report an extensive examination of the properties of two major indexes G_ST and D under non - equilibrium conditions by theoretical deduction under the infinite allele model (IAM) and simulation under the stepwise mutation model (SMM). Several properties of G_ST and D valid under both SMM and IAM, which have not been recognized under the equilibrium perspective, were unveiled. First, if gene flow is very weak (e. g., m < 10~( - 4)), G_(ST), like D, also takes a fairly long time to reach equilibrium if mutation rate is not very large. When G_(ST) (D) is in equilibrium depends on when H_S and H_T are both in equilibrium. Under IAM and complete isolation, this is determined by the product of μ and t: G_(ST) will not approach equilibrium as long as μt 《 1. Under SMM, 10 ~(-4) appears to be the rough threshold migration rate; when m < 10~(-4), G_(ST) approaches equilibrium much slower and later than H_S, whereas the opposite is true when m > 10~(-4). Second, contrary to the popular belief, μ 《 mis neither an obligatory request for G_(ST) to be an effective differentiation measure nor a sufficient condition for using G_(ST) to estimate gene flow level (Nm), if G_(ST) is not yet approaching equilibrium. Third, under SMM (but not IAM) and complete isolation, when population size is large (e. g., > 1000), mutation rate shows a great impact on G_(ST) but only a mild influence on D; hence D can be much less sensitive to mutation rate heterogeneity than G_(ST) in certain situations. Fourth, whatever the level of gene flow, drift plays a dominant role on GST, whereas gene flow appears to have a stronger influence on D when subpopulations exchange individuals (even only infrequently). Moreover, G_(ST) appears to have a larger power to detect recent population genetic events than D. Finally, it is the migration rate (m), not the absolute number of migrants (Nm), that determines the speed at which G_(ST) or D approaches equilibrium. These results suggest that G_(ST) and D bear unique revealing powers under non-equilibrium conditions.
发挥学科综合、交叉融合的优势保持自身特色促进急诊医学发展

我国文学名著《三国演义》开篇第一句话即“天下大事,分久必合,合久必分”,指的是政治、人间世事和人世间的沧海桑田。具体到医学也是这样,以当初的综合性,由于各个专业学科的发展逐渐分科越来越细。直至今天,有的学科不但是三级,而且是单病种形成专科态势。而诊治的医师也由于天天看一种病或一类病,在大学学习的基础知识或在三年、五年住院医师轮转学的大内科、大外科知识逐渐缩水或淡忘,不用则废。仅仅知道日常处理这一类病或此专病的知识,而对非自己从事的专业或更为基础的涉及多学科的问题,往往知之甚少。这样就逐渐形成了管状视野,只见树林,不见森林。而现代医学发展,由于诊治手段措施的进步,使人的生命延长,进入老龄化社会。其特点是老年人有多种慢性病和基础病且常年服药,干预治疗的因素多。这就形成了一个尴尬的局面,一旦某一慢性病急性发作或得一种新病,由新发病引发基础疾病导致多器官受累,不知道到哪个专科救治。同时由于医疗政策的规定很具体,如缩短住院天数、加快床位周转、单病种付费、推行临床路径、诊断相关组(diagnosis related groups,DRGs)实施和考核,使得三级医院各专科医师面对有多种合并症、并发症的危重性疾病,往往手足失措,不知从何下手,误诊误治情况时有发生。基于此,各专科分会及有识之士大声疾呼要求重视相关学科的知识学习和积累,甚至要求学科专业重新整合、融合、交叉。其主要宗旨:①回归认识疾病发生、发展的规律,从有机体的整体观诊治疾病,而非一律采用“头痛医头,脚痛医脚,见兔放鹰”的救治原则。 ②利于在学科相互之间的交叉部位,在新的更高层面上进行科学研究探索,在更高的层面上进行整合以实现新的突破。③有利于对疾病的诊治,除治疗主要疾病外,兼顾一般医学问题或其他学科问题,这样既方便患者又节省费用,也利于医药卫生体制改革。
塞姆利基森林病毒复制子DNA疫苗研究进展

甲病毒(Alphavirus)是披膜病毒科(Togavirusfamily)的成员,是单股、正链的RNA病毒,包括塞姆利基森林病毒(Semliki forest virus,SFV)、辛德毕斯病毒(Sindbis virus,SIN)和委内瑞拉马脑炎病毒(Venezuelan equineencephalitis virus,VEE)等30个成员。20世纪80年代末,随着这些病毒的cDNA感染性克隆的建立,相继开展了以甲病毒复制子为基础载体的研究,利用复制子特有的RNA复制酶提高翻译模板mRNA的量来实现高水平表达的目的,以改造出相应的甲病毒载体,已被广泛地应用于基因治疗和分子生物学的研究,并在疫苗领域中受到关注。
An analysis of the radar backscatter from oil-covered sea surfaces using moment method and Monte-Carlo simulation: preliminary results

An analysis of the radar backscattering from the ocean surface covered by oil spill is presented using a microwave scattering model and Monte-Carlo simulation. In the analysis, a one-dimensional rough sea surface is numerically generated with an ocean waveheight spectrum for a given wind velocity. A two-layered medium is then generated by adding a thin oil layer on the simulated rough sea surface. The electric fields backscattered from the sea surface with two-layered medium are computed with the method of moments (MoM), and the backscattering coefficients are statistically obtained with N independent samples for each oil-spilled surface using the Monte-Carlo technique for various conditions of surface roughness, oil-layer thickness, frequency, polarization and incidence angle. The numerical simulation results are compared with theoretical models for clean sea surfaces and SAR images of an oil-spilled sea surface caused by the Hebei (Hebei province, China) Spirit oil tanker in 2007. Further, conditions for better oil spill extraction are sought by the numerical simulation on the effects of wind speed and oil-layer thickness at different incidence angles on the backscattering coefficients.
Endoscopic findings in patients with Schatzki rings:Evidence for an association with eosinophilic esophagitis

AIM:To investigate endoscopic findings in patients with Schatzki rings(SRs) with a focus on evidence for eosinophilic esophagitis(EoE).METHODS:We consecutively approached all adult patients scheduled for elective outpatient upper endoscopy for a variety of indications at the German Diagnostic Clinic,Wiesbaden,Germany between July 2007 and July 2010.All patients with endoscopically diagnosed SRs,defined as thin,symmetrical,mucosal structures located at the esophagogastric junction,were prospectively registered.Additional endoscopic findings,clinical information and histopathological findings with a focus on esophageal eosinophilia(≥ 20 eosinophils/high power field) were recorded.The criteria for active EoE were defined as:(1) eosinophilic tissue infiltration ≥ 20 eosinophils/hpf;(2) symptoms of esophageal dysfunction;and(3) exclusion of other causes of esophageal eosinophilia.Gastroesophageal reflux disease was excluded by proton pump inhibitor treatment prior to endoscopy.The presence of ≥ 20 eosinophils/hpf in esophageal biopsies in patients that did not fulfil the criteria of EoE was defined as esophageal hypereosinophilia.RESULTS:A SR was diagnosed in 171(3.3%;128 males,43 females,mean age 66 ± 12.9 years) of the 5163 patients that underwent upper gastrointestinalendoscopy.Twenty of the 116 patients(17%) from whom esophageal biopsies were obtained showed histological hypereosinophilia(≥ 20 eosinophils/hpf).Nine of these patients(8 males,1 female,mean age 49 ± 10 years) did not fulfill all diagnostic criteria of EoE,whereas in 11(9%) patients with ≥ 20 eosinophils/hpf,a definite diagnosis of EoE was made.Three of the 11 patients(27%) with definite EoE had no suspicious endoscopic features of EoE.In contrast,in the 25 patients in whom EoE was suspected by endoscopic features,EoE was only confirmed in 7(28%) patients.Patients with EoE were younger(mean age 41.5 ± 6.5 vs 50.5 ± 11.5 years,P = 0.012),were more likely to have a history of allergies(73% vs 29%,P = 0.007) and complained more often of dysphagia(91% vs 34%,P = 0.004) and food impaction(36% vs 6%,P = 0.007) than patients without EoE.Endoscopically,additional webs were found significantly more often in patients with EoE than in patients without EoE(36% vs 11%,P = 0.04).Furthermore,the SR had a tendency to be narrower in patients with EoE than in those without EoE(36% vs 18%,P = 0.22).The percentage of males(73% vs 72%,P = 1.0) and frequency of heartburn(27% vs 27%,P = 1.0) were not significantly different in both groups.The 9 patients with esophageal hypereosinophilia that did not fulfil the diagnostic criteria of EoE were younger(mean age 49 ± 10 years vs 58 ± 6 years,P = 0.0008) and were more likely to have a history of allergies(78% vs 24%,P = 0.003) than patients with<20 eosinophils/hpf.Predictors of EoE were younger age,presence of dysphagia or food impaction and a history of allergies.CONCLUSION:A significant proportion of patients with SRs also have EoE,which may not always be suspected according to other endoscopic features.
上腔静脉回流无阻断人工血管置换术在肺癌外科中的应用

所有肺癌侵犯上腔静脉(SVC患者中,约1/3需要进行SVC完全切除及置换手术~([1])。此类手术多在SVC回流阻断的情况下施行,SVC阻断时间较长会出现严重的脑水肿~([2-4])。我们在术中应用建立临时无名静脉与右心耳旁路的方法,避免了血管置换过程中SVC回流被阻断,保证了手术的安全,现总结报道如下。
工业加氢装置的6种腐蚀与选材基准

工业加氢装置易受介质腐蚀,常发生生产事故:胜利炼厂脱硫再生塔顶冷却器焊缝发生20余处开裂、回流罐发生800余处氢鼓泡、3台加氢反应产物后冷器内1/2以上浮头面30CrMo螺栓发生断裂,扬子石化芳烃燃气分离罐因H_2S腐蚀而导致底部严重减薄、2005年1月投用的1.0×l0~6 t/a中压加氢裂化装置2006年多次发生泄漏,大连石化加氢裂化装置加氢反应器出口347承插焊结构管线使用不到1年发生开裂泄漏。以上这类失效事故可通过选材和维护加以避免,以下就各种腐蚀形式、机理及措施进行归纳、说明。
The regulation of myoblast plasticity and its mechanism

The development of skeletal muscle is a highly regulated,multi-step process in which pluripotent mesodermal cells give rise to myoblasts that subsequently withdraw from the cell cycle and differentiate into myotubes as well as myofibers.The plasticity of myoblasts plays a critical role in maintaining skeletal muscle structure and function by myoblast activation,migration,adhesion,membrane reorganization,nuclear fusion,finally forming myotubes/myofibers.Our studies demonstrate that the local hypoxic microenvironment,a great diversity of regulatory factors such as IL-6 superfamily factors(IL-6,LIF,CNTF) and TGF-β1 could regulate the myoblast plasticity.The aim of this paper is to review the previous studies focused on the regulation of myoblast plasticity and its mechanism in our laboratory.Knowledge about the microenvironment or factors involved in regulating the myoblast plasticity will help develop the prevention and cure measures of skeletal muscle diseases.
Equilibrium configurations of the tethered three-body formation system and their nonlinear dynamics

This paper considers nonlinear dynamics of tethered three-body formation system with their centre of mass staying on a circular orbit around the Earth, and applies the theory of space manifold dynamics to deal with the nonlinear dynamical behaviors of the equilibrium configurations of the system. Compared with the classical circular restricted three body system, sixteen equilibrium configurations are obtained globally from the geometry of pseudo-potential energy surface, four of which were omitted in the previous research. The periodic Lyapunov orbits and their invariant manifolds near the hyperbolic equilibria are presented, and an iteration procedure for identifying Lyapunov orbit is proposed based on the differential correction algorithm. The non-transversal intersections between invariant manifolds are addressed to generate homoclinic and heteroclinic trajectories between the Lyapunov orbits. (3,3)- and (2,1)-heteroclinic trajectories from the neighborhood of one collinear equilibrium to that of another one, and (3,6)- and (2,1)-homoclinic trajectories from and to the neighborhood of the same equilibrium, are obtained based on the Poincare mapping technique.
Study of Biomass Calcite as Fine Aggregate of Concrete

The possibility of using crushed oyster shell to partly replace the fine aggregate of concrete was evaluated. The compressive strength and slump of concrete mixture with different amount of crushed oyster shell were tested and thus the appropriate dosage was determined. Additionally, the compatibility with super plasticizer and the stability in Na2SO4 solution were also discussed to prove the feasibility of oyster shell as fine aggregate of concrete. The microstructure of concrete was observed with XRD and SEM techniques. This research provides the basis for the application of waste biomass calcite.
Extraction and Crystal Structure of Pedunculoside

The title compound of pedunculoside (C_(36)H_(58)O_(10)), a main active triterpenoid saponin of Ilicis Rotundae Cortex, was isolated from the dried bark of Ilex rotunda Thunb., and characterized by X-ray diffraction analysis. It crystallizes in the orthorhombic system, space group P2_12_12_1 with C_(36)H_(58)O_(10), a=13.046(3), b=21.038(4), c=28.413(6) A, V=7798.28(3) A~3, Z=8, D_c=1.109 g/cm~3, M_r=650.82, F(000)=2832, and μ=0.080 mm~(-1). The final R=0.0765 and wR=0.1736 for 15117 observed reflections (I > 2σ(I)). The molecular crystal structure of pedunculoside shows relative stereochemistry of 3β,19α,23-trihydroxy-urs-12-en-28-oic acid-28-O-β-D-glucopy-ranosyl ester. The molecule is composed of one glucopyranosyl ring and five six-membered rings with ring junctures of A/B trans, B/C trans, and D/E cis. There are two C_(36)H_(58)O_(10) molecules in an asymmetric unit, and the title compound is stacked into a 2D layer structure through hydrogen bonds.
强直性脊柱炎可溶性CD28/CD80/CD86/CD152的表达

强直性脊柱炎(ankylosing spondylitis, AS)是一种自身免疫功能紊乱,HLA-B27表达增高的慢性炎症疾病,协同共剌激免疫分子失调是其主要诱因。本文应用酶联免疫吸附试验(ELISA)和补体依赖的微量淋巴细胞毒试验(MLCT)研究强直性脊柱炎患者外周血清中可溶性CD28/CD80/CD86/CD152的表达。
Genomic Analysis of the Natural Population of Wheat dwarf virus in Wheat from China and Hungary

During the last decade, the leafhopper transmitted Wheat dwarf virus (WDV) has become a serious problem both in northwestern China and Hungary. In order to study the molecular diversity and population structure of WDV in these two countries, 39 Chinese isolates and 16 Hungarian isolates were collected from different regions of China and Hungary, and their genomes were sequenced. All isolates belonged to the wheat strain of WDV and showed limited genetic diversity. The highest and lowest nucleotide sequence identities among isolates from China and Hungary were 99.9 and 90%, respectively. In all isolates, the lowest nucleotide sequence identity was 89.5% between MO10-1 and KP10-5, which were collected from Martonvasar and Kompolt, Hungary. Phylogenetic analyses showed the genome sequences of 55 WDV isolates belong to two big clades, but no clear correlation to geographical location. Population difference analyses indicated that the Chinese and Hungarian WDV populations have no significant difference. The regions in WDV genome with relatively low nucleotide diversities represented protein coding regions suggested that these regions evolved under negative selection, and might be one of the causes restricting the number of genetic variants.
Biomass Components and Environmental Controls in Ningxia Grasslands

Grassland plays an important role in the global carbon cycle and climate regulation. However, there are still large uncertainties in grassland carbon pool and also its role in global carbon cycle due to the lack of measured grassland biomass at regional scale or global scale with a unified survey method, particular for below-ground biomass. The present study, based on a total of 44 grassland sampling plots with 220 quadrats across Ningxia, investigated the characteristics of above-ground biomass (AGB), below-ground biomass (BGB), litter biomass (LB), total biomass (TB) and root:shoot ratios (R:S) for six predominantly grassland types, and their relationships with climatic factors. AGB, BGB, LB and TB varied markedly across different grassland types, the median value ranging from 28.2-692.6 g m~(-2) for AGB, 130.4-2 036.6 g m~(-2) for BGB, 9.2-82.3 g m~(-2) for LB, and 168.0-2 681.3 g m~(-2) for TB. R:S showed less variation with median values from 3.2 to 5.3 (excluding marshy meadow). The different grassland types showed similar patterns of biomass allocation, with more than 70% BGB for all types. There is evidence of strong positive effects associated with mean annual precipitation (MAP) and negative effects associated with mean annual temperature (MAT) on AGB, BGB, and LB, although both factors have the opposite effect on R:S.
Selection of Submergence Tolerant Homozygous Line by STS Marker and Twice Submergence Stress

One sequence tagged site marker Subl-1 and twice submergence stress method were used in selection of submergence tolerant homozygous line from Sub-1BS, a submergence tolerant, bentazon sensitive and photoperiod-sensitive and/or thermo-sensitive genic male sterile line that developed by our laboratory. The results revealed that the original Sub-IBS was heterozygous in SublA-1 locus even though it was identical in almost all of agronomical traits and the segregation of SublA-1 was in accordance with Mendelian law based on chi-square test. And then the original Sub-IBS was divided into two groups: one was of Sub1A-1 introgression and the other was not; and the two groups were tested by twice submergence stress method. After the first submergence stress that lasted for 12 d, the average plant heights were significant difference at the 1% level between the two groups. After recovery for 10 d, the second submergence stress sustained for 18 d was carried on; and the group with SublA-1 gene was found apparently tolerant than the other group in submergence tolerance.
Effects of RNAi Silencing of SSIII Gene on Phosphorus Content and Characteristics of Starch in Potato Tubers

The sense and antisense fragments of the soluble starch synthase (SSIII) gene and the intron fragment of somatic embryogenesis receptor-like kinase (SERKl) gene were cloned from potato using PCR techniques. The RNAi plant expression vectors pBI-SSIII-RNAi and _pBIC-SSIII-RNAi were constructed which containing fusion fragment of "sense fragment-intron-antisense fragment" driven by the constitutive expression promoter CaMV 35S and the tuber-specific expression promoter CIPP, respectively. The putative transgenic plants of potato cultivars Kexin-1 and Kexin-4 were obtained using Agrobacterium-mediated transformation method. PCR assay showed that the interference fragment of SSIII gene was integrated into potato genome. The RT-PCR analysis showed that the expression of SSIII gene was repressed apparently on the transcription level. Starch granules of the transgenic potato plants were different in morphology and became cracked in starch granule centre compared with the non-transgenic control plants. The amylose content of starch was increased by 2.68-29.05%, amylopectin to amylose ratio of starch had declined significantly, and the phosphorus content of the starch of the transgenic plants was reduced 9.94-58.36% compared with control plants. The results could provide certain foundation for improvement of potato starch quality.
以需求为导向整合高职高专类临床医学教学内容的探讨

随着社会经济的发展,社会环境的变化,人群疾病谱也有所改变,对临床医学专业学生的教学内容也应随之发生改变。但以前对高职高专类临床医学专业学生临床知识的教学内容主要根据全国临床医学教学大纲和执业(助理)医师考试大纲来进行教学,没有结合区域特有的经济和环境情况所带来的不同的疾病谱来选择常见病、多发病作为重点教学内容。而且随着医改的进行,乡镇卫生院的职能也从以前重点承担临床工作任务转向以公共卫生服务为主,综合提供预防、保健和基本医疗等服务。因此,为适应社会的发展,必须以需求为导向整合高职高专类临床医学教学内容,突出临床专业的特色和应用性。笔者在总结以往教学改革经验的基础上,通过对三峡库区农村基层临床医生的问卷调查,从临床医生应掌握哪些常见病、多发病的诊治及拥有哪些公共卫生服务能力的角度出发,试图构建新的高职高专类临床医学专业重点教学内容,用于指导教师教学。
人工全髋置换术治疗陈旧性髋臼骨折伴创伤性关节炎

本院自2009年2月以来收治12例陈旧性髋臼骨折患者,通过对髋臼实施复位、矫形、植骨、造盖等大体恢复髋臼窝的形态,行人工全髋置换,经定期随访,效果满意,现报道如下。