mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Synthesis,Crystal Structure and Cyclic Voltametric Property of Cu(Ⅱ) Complex with 4,4'-Bipy and α-Pyridine Carboxylate

The complex [Cu(bipy)(pc)(H_2O)(ClO_4)]·H_2O(1)(bipy:4,4'-bipyridine;Hpc:α-pyridine carboxylic acid) has been synthesized and characterized by single crystal X-ray diffraction method and elemental analysis.It crysrallizes in the Monoclinic space group P2_1/n.The crystal structure reveals that Cu(Ⅱ) centre adopts a pseudo octahedral geometry.Ligand 4,4'-bipyridine as a bridge coordinates to two different Cu(Ⅱ) center to form a onedimensional zigzag chain.One dimensional chains are linked by C-H…O hydrogen bonding interactions to form two-dimensional layer.Layers are connected by O…H-O hydrogen bond to generate three-dimensional structure.The cyclic voltametric behavior of complex 1 is also investigated.
加味当归四逆汤治疗膝骨性关节炎的临床研究

目的:探讨加味当归四逆汤内服治疗膝关节骨性关节炎的临床疗效。方法:将120例患者随机分为两组,治疗组予以加味当归四逆汤内服,对照组予追风透骨丸口服。并制定临床主症积分标准,两周后对纳入本研究的膝关节骨性关节炎患者进行关节疼痛、关节压痛、关节功能、综合疗效等方面的评估。结果:治疗组总有效率为91.67%,对照组总有效率为80.00%。经统计学处理P＜0.05,两组有显著性差异。结论:通过应用加味当归四逆汤内服治疗膝关节骨性关节炎,确实能起到养血通脉、温经散寒、改善关节功能的作用。
花椒挥发油的抗炎、镇痛作用

目的:观察和验证花椒挥发油抗炎、镇痛的药理作用。方法:采用小鼠耳廓肿胀法、腹腔毛细血管通透性试验、滤纸性肉芽肿试验、热板法、扭体法进行实验观察。结果:①花椒挥发油不同剂量灌胃给药,在给药7、14、21d对二甲苯引起的小鼠耳廓肿胀有显著的抑制作用(P<0.01);②花椒挥发油不同剂量灌胃给药14d对醋酸(HAc)所致小鼠腹腔毛细血管通透性作用不明显;③花椒挥发油不同剂量灌胃给药14d对滤纸性肉芽肿无明显抑制作用;④花椒挥发油不同剂量灌胃给药3、6d对于小鼠热刺激导致的疼痛无显著性影响,同样时间点对醋酸导致的小鼠腹部疼痛均有显著的抑制作用。结论:①花椒挥发油抗炎效果显著;②花椒挥发油对醋酸(HAc)所致小鼠腹腔毛细血管通透作用不明显;③花椒挥发油对滤纸性慢性肉芽肿无明显的抑制作用;④花椒挥发油对小鼠热刺激所致的急性疼痛作用不明显;花椒挥发油对醋酸引起的小鼠腹部疼痛有抑制作用。
葛根芩连汤有效部位组方体内外抗轮状病毒作用研究

目的:观察葛根芩连汤及其有效部位组方体内外抗人A组Wa株轮状病毒的作用。方法:体外接种人A群Wa株轮状病毒于恒河猴胚肾细胞(MA104),结合细胞病变(CPE)和噻唑蓝比色法(MTT)测定各组药物对病毒的抑制浓度,同时以新生昆明小鼠感染轮状病毒致腹泻为模型,观察药物对各组动物腹泻率和腹泻程度的影响。结果:各组药物对该毒株的半数抑制浓度(IC_(50))不全相同。体内实验结果显示,给药干预后,各组动物的腹泻率和腹泻指数均有不同程度降低。结论:葛根芩连汤及其有效部位组方通过阻止病毒进入宿主细胞,抑制该毒株的生物合成从而发挥抗病毒的作用。两者对新生昆明小鼠的轮状病毒感染性腹泻均有一定疗效,以有效部位组方疗效较好。
广西不同地域五、七叶绞股蓝中三萜皂苷含量差异研究

目的:探讨影响绞股蓝药用有效成分三萜皂苷含量的因素,为绞股蓝种植、制药提供科学建议。方法:采用超声波提取和分光光度法,分析比较不同品种、不同组织、不同产地绞股蓝间总三萜皂苷含量的差异。结果:七叶绞股蓝叶片三萜皂苷含量(6.78%)显著高于五叶绞股蓝(3.69%),绞股蓝叶片中三萜皂苷的平均含量(4.72%)显著高于茎的平均含量(1.85%),广西南部产五叶绞股蓝叶片三萜皂苷含量(4.1%)高于广西北部产五叶绞股蓝(3.6%)。结论:为获得含量更高的三萜皂苷,实现最大经济效益,建议药农种植七叶绞股蓝,药材收购时宜叶茎分开。
老年冠心病患者介入治疗术后支架膨胀不全的光学相干断层成像1例

药物洗脱支架膨胀不全是支架内再狭窄和支架内血栓形成的重要危险因素. 支架膨胀不全往往与术者的技术(如选择球囊直径较小、扩张压力较低、扩张时间较短)和冠状动脉病变(如血管直径、斑块容积、斑块成分，特别是钙化病变)有关，对于特殊病变即使是选择大球囊、高压力和长时间扩张也不能使支架达到“理想”的扩张状态. 老年冠心病患者由于冠状动脉钙化往往导致支架膨胀不全，但本例LAD病变处无明显钙化斑块，OCT检查为纤维斑块，且在支架置入前行充分预扩张、置入后经高压后扩张，术后9 d患者再次出现心绞痛等临床症状，拟除外支架内亚急性血栓形成行CAG及OCT检查，未发现血栓，见LAD支架局部膨胀不全且有部分支架小梁贴壁不良. 分析原因与LAD弥漫长病变且成角、病变周围大量纤维斑块有关，不除外后扩球囊直径选择较小、支架弹性回缩及其他因素. 根据血管内超声指导的“理想”支架置入标准，支架膨胀良好的定义指支架置入后最小管腔横截面积／平均参考血管腔截面积≥0．8. 本例 OCT发现支架置入狭窄处横截面与平均参考血管腔截面积比值为0．69，提示膨胀不全. 其主要的预防措施为充分预扩张、斑块移除等方法，主要的补救措施则包括充分高压后扩张、切割球囊使用、旋磨技术等. 该例经大一号高压球囊充分扩张后管腔截面积明显扩大，与平均参考血管腔截面积比值为0．81，达“理想”支架置入标准. 总之，上述病例充分说明了OCT在判断支架膨胀不全的准确性及对”理想”支架置入有重要指导价值.
Microstructure and mechanical properties of forged Al-7.1Zn-1.1Mg-1.6Cu-0.14Zr alloy after two-step ageing treatment at 120 and 170℃

The tensile properties,electrical conductivity,and microstructure of the forged Al-7.1Zn-1.1Mg-1.6Cu-0.14Zr alloy were investigated after a two-step ageing treatment at 120 and 170℃.The results indicate that the strength of the alloy reaches the peak value at 170℃ for 1 h during the second step ageing and then decreases sharply.However,the electrical conductivity value increases continuously with the second ageing time increasing.The fracture mechanism of the alloy is intergranular fracture for 1 h and then changes to dimple transgranular fracture later,and the toughness of the alloy is improved significantly.The phases of η' and η are major precipitates in the alloy under the two-step ageing condition.Discontinuous grain boundary precipitates and precipitate-free zones along the grain boundary are clearly observed.
气相色谱法测定金银花中芳樟醇的含量

目的建立气相色谱法测定金银花中芳樟醇的含量. 方法采用Agilent HP-5毛细管色谱柱(30 m×0.32 mm×0.25μm)为固定相, FID检测, 检测器温度240℃, 进样口温度240℃, 程序升温, 氮气为载气, 载气流速2.0 mL/min. 结果在该色谱条件下, 芳樟醇在57.35917.60μg/mL范围内线性关系良好, 平均回收率为97.9%, RSD为1.16%. 结论该方法简单、灵敏、回收率和重现性好, 可作为金银花药材中芳樟醇的质量控制方法.
周仲瑛教授“复合病机论”探析

国医大师周仲瑛教授一直重视对病机学说的创新研究, 倡导基于病机证素进行辨证论治, 其理论核心是复合病机论. 通过研究认为, 多因复合、多病位复合是复合病机的临床特征, 而邪正交争、多病势复合所致的病机转化、因果夹杂则是复合病机形成的内在基础. 通过分析周老对内科急难病证重视病机转化与复合病机的学术思想, 提出基于复合病机理论的临床应用思路并分析了学术意义.
全球7级以上大地震及中国4级以上地震目录(2009年7～9月)

应广大读者要求,我们定期公布全球7级以上大地震和中国4级以上地震目录。地震参数取自中国地震台网中心的“中国地震速报台网观测报告”和“中国地震月报目录”及“中国数字地震台网观测报告”。其中:发震时间为北京时(BJT);震中位置除给出经纬度外还给出了参考地区名,以供查阅时参考;MS为面波震级、ML为近震震级;MW为矩震级(取自美国PDE报告);mb为短周期体波震级;mB为中长周期体波震级。
基于省级基础地理信息数据库制图的关键技术研究

基于基础地理信息数据库的自动快速制图,是目前地图学研究的关键问题之一。首先研究地理信息数据与地形图之间的逻辑关系,再根据国标图式表达规范,采用面向制图和GIS的省级基础地理信息分类编码体系,开发了相应的符号库系统,以自动或半自动化方式实现了地图的符号化,解决了符号配置过程中要素之间的各种关系处理、注记的自动配置以及自动整饰等关键技术,实现了基于省级基础地理信息数据库自动快速制图系统。
基于PDA的1∶10000比例尺地形图野外调绘

随着移动信息设备的发展,GIS逐步进入后PC发展阶段,嵌入式GIS应用不断增加,因此开发基于移动信息设备的地理信息软件,可以更加方便地进行地理信息数据的采集、更新及导航应用。
一种利用NDVI辅助提取植被信息的改进方法

为提高遥感影像中不同覆盖度植被信息由栅格到矢量的转化效率和准确度,改进了常用的NDVI辅助提取植被信息方法,即根据NDVI与植被覆盖度之间的关系自动提取不同覆盖度的植被信息。为检验该方法的效果,利用试验区一组TM影像进行植被信息的提取试验。结果表明,该方法与常用方法相比能有效地增强不同覆盖度植被边缘信息,提高了植被边缘信息栅格矢量化的效率和准确度。
智能路面弯沉检测系统的设计与研究

将3G通信技术、GPS技术、落锤式弯沉仪路面检测技术相结合,实现了路面弯沉的快速检测和定位,同时对路面检测的各种数据进行实时动态管理与分析研究,构成路面智能弯沉检测系统。通过该系统的研究与设计,可为将来建立集路面弯沉、车辙和平整度等多种路面数据于一体的路面质量综合评价系统打下基础,从而真正实现多数据源的智能路面检测系统,为道路科学养护提供智能分析与应用。
基于GIS的公交数据模型研究及换乘算法实现

针对公交出行中的一些实际问题,设计一种用GIS矢量数据结构表达公交网络数据位置和关系的公交数据模型,在此基础上实现了以最少换乘次数为第一目标、最少途经站数为第二目标的公交换乘算法。该算法不仅解决了步行换乘、环路换乘等问题,而且优化了换乘点的选择。此外,利用数据库和空间数据引擎的索引和快速检索性能,并结合基于内存的查询、集合运算等高效处理机制,有效地解决了算法的效率问题,并应用于实践。
A Kind of Weingarten Surfaces in E~3 with Prescribed Principal Curvatures

In this paper, we construct a kind of Weingarten surfaces in E~3 and study its geometric properties. We first derive an explicit diffierential relationship between the principal curvatures of them. Then we prove an existence theorem of this kind of surfaces with prescribed principal curvatures. At last, we present two examples involving the rotation surfaces as the special case, and present several figures to the second example.
SNL fibroblast feeder layers support derivation and maintenance of human induced pluripotent stem cells

Induced pluripotent stem(iPS) cells can be derived from human somatic cells by cellular reprogramming.This technology provides a potential source of non-controversial therapeutic cells for tissue repair,drug discovery,and opportunities for studying the molecular basis of human disease.Normally,mouse embryonic fibroblasts(MEFs) are used as feeder layers in the initial derivation of iPS lines.The purpose of this study was to determine whether SNL fibroblasts can be used to support the growth of human iPS cells reprogrammed from somatic cells using lentiviral expressed reprogramming factors.In our study,iPS cells expressed common pluripotency markers,displayed human embryonic stem cells(hESCs) morphology and unmethylated promoters of NANOG and OCT4.These data demonstrate that SNL feeder cells can support the derivation and maintenance of human iPS cells.
Methylation patterns in 5' terminal regions of pluripotency-related genes in bovine in vitro fertilized and cloned embryos

In order to investigate DNA methylation profiles of five pluripotency-related genes (Oct4, Sox2, Nanog, Rexl and Fgf4) during bovine maternal to zygotic transition (MZT) in both in vitro fertilized (IVF) and nuclear transfer (NT) embryos, sodium bisulfite sequencing method was used to detect DNA methylation levels, accompanied by the statistical analysis of embryo developmental rates. The results showed that Oct4, Nanog, Rexl and Fgf4 were respectively demethylated by 25.22% (P < 0.01), 3.84% (P > 0.05), 31.82% (P < 0.01) and 10% (P > 0.05) while Sox2 retained unmethylation during MZT in IVF embryos. By contrast, Oct4 and Rexl respectively underwent demethylation by 23.04% (P < 0.01) and 6.02% (P > 0.05), and, reversely, Sox2, Nanog and Fgf4 respectively experienced remethylation by 0.84% (P > 0.05), 5.39% (P > 0.05) and 5.46% (P > 0.05) during MZT in NT embryos. Interestingly, the CpG 14 site of Sox2 was specifically methylated in both 8-cell and morula NT embryos. In addition, the development of blastocysts between IVF and NT embryos showed no significant difference. DNA methylation analysis showed that only Oct4 and Sox2 underwent the correct methylation reprogramming process, which may be responsible for the development of blastocysts of NT embryos to a certain extent. In conclusion, the five genes respectively experienced demethylation to different extents and incomplete DNA methylation reprogramming during bovine MZT in both IVF and NT embryos, suggesting that they may be used as indicators for bovine embryo developmental competence.
Characteristics of contaminants in water and sediment of a constructed wetland treating piggery wastewater effluent

Constructed wetland (CW) is the preferred means of controlling water quality because of its natural treatment mechanisms and function as a secondary or tertiary treatment unit. CW is increasingly applied in Korea for secondary effluent of livestock wastewater treatment. This study was conducted to recognize the characteristics of contaminants in the accumulated sediment at the bottom soil layer and to reduce the phosphorus release from sediments of the free water surface CW for the treatment of secondary piggery wastewater effluent from a livestock wastewater treatment facility. The results revealed that the dominant phosphorus existence types at near the inlet of the CW were non-apatite phosphorus (59%) and residual phosphorus (32%) suggesting that most of the particles of the influent are made up of inorganic materials and dead cells. Sediment accumulation is important when determining the long-term maintenance requirements over the lifetime of CW. Continuous monitoring will be performed for a further assessment of the CW system and design.
Evaluation of non-point source pollution reduction by applying Best Management Practices using a SWAT model and QuickBird high resolution satellite imagery

This study evaluated the reduction Effect of non-point source pollution by applying best management practices (BMPs) to a 1.21 km~2 small agricultural watershed using a SWAT (Soil andWater Assessment Tool) model. Two meter QuickBird land use data were prepared for the watershed. The SWAT was calibrated and validated using daily streamflow and monthly water quality (total phosphorus (TP), total nitrogen (TN), and suspended solids (SS)) records from 1999 to 2000 and from 2001 to 2002. The average Nash and Sutcliffe model efficiency was 0.63 for the streamflow and the coefficients of determination were 0.88, 0.72, and 0.68 for SS, TN, and TP, respectively. Four BMP scenarios viz. the application of vegetation filter strip and riparian buffer system, the regulation of Universal Soil Loss Equation P factor, and the fertilizing control amount for crops were applied and analyzed.
Experimental and thermodynamic investigation of heavy metal partitioning influenced by flue gas moisture and chlorine content during waste incineration

The impact of moisture on the partitioning of the heavy metals including Pb, Zn, Cu and Cd in municipal solid waste (MSW) was studied in a laboratory tubular furnace. A thermodynamic investigation using CHEMKIN software was performed to compare the experimental results. Simulated waste, representative of typical MSW with and without chlorine compounds, was burned at the background temperature of 700 and 950℃, respectively. In the absence of chlorine, the moisture content has no evident effect on the volatility of Pb, Zn and Cu at either 700 or 950℃, however, as flue gas moisture increasing the Cd distribution in the bottom ash increased at 700℃ and reduced at 950℃, respectively. In the presence of chlorine, the flue gas moisture reduced the volatility of Pb, Zn and Cu due to the transformation of the more volatile metal chlorides into less volatile metal oxides, and the reduction became significant as chlorine content increase. For Cd, the chlorine promotes its volatility through the formation of more volatile CdCl_2. As a result, the increased moisture content increases the Pb, Zn, Cu and Cd concentrations in the bottom ash, which limits the utilization of the bottom ash as a construction material. Therefore, in order to accumulate heavy metals into the fly ash, MSW should be dried before incineration.
Anaerobic benzene biodegradation by a pure bacterial culture of Bacillus cereus under nitrate reducing conditions

A pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil. An analysis of the 16S rDNA gene sequence, morphological and physiological characteristics showed that the isolated strain is a member of the genus Bacillus cereus. The biodegradation performance of benzene by B. cereus was evaluated, and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/L. The metabolites of anaerobic nitrate-dependent benzene oxidation by strain B. cereus were identified as phenol and benzoate. The results of substrate interaction between binary combinations for benzene, phenol and benzoate showed that the simultaneous presence of benzene stimulated the degradation of benzoate, whereas the addition of benzene inhibited the degradation of phenol. Benzene degradation by B. cereus was enhanced by the addition of phenol and benzoate, the enhanced effects were more pronounced at higher concentration. To our knowledge, this is the first report that the isolated bacterial culture of B. cereus can efficiently degraded benzene under nitrate reducing conditions.
Particle properties in granular activated carbon filters during drinking water treatment

The elemental composition and bacteria attached in particles were investigated during granular activated carbon (GAC) filtration. The experimental results show that trapped influent particles formed new, larger particles on GAC surface. The sloughing of individuals off GAC surface caused an increase in effluent particles in the size range from 5 to 25 μm. The selectivity for element removal in GAC filters caused an increasing proportion of metallic elements in the effluent particles. The distribution of molar ratio indicated a complicated composition for large particles, involving organic and inorganic substances. The organic proportion accounted for 40% of total carbon attached to the particles. Compared with dissolved carbon, there was potential for the formation of trihalomethanes by organic carbon attached to particles, especially those larger than 10 μm. The pure carbon energy spectrum was found only in the GAC effluent and the size distribution of carbon fines was mainly above 10 μm. The larger carbon fines provided more space for bacterial colonization and stronger protection for attached bacteria against disinfection. The residual attached bacteria after chorine disinfection was increased to 102-103 CFU/mL within 24 hours at 25℃.
Biosorption of zinc(II) from aqueous solution by dried activated sludge

The biosorption potential of dried activated sludge as a biosorbent for the removal of zinc(II) from aqueous solution was investigated. Studies on the effects of initial pH, contact time, initial zinc ion concentration, and adsorbent dosage on the biosorption processes were conducted, and the equilibrium data were modeled by the Langmuir and Freundlich isotherms. The Langmuir isotherm model (R~2 = 0.999) was proved to fit the equilibrium data much better than the Freundlich isotherm model (R~2 = 0.918). The monolayer adsorption capacity of dried activated sludge for zinc(II) was found to be 17.86 mg/g at pH of 5 and 25℃. The kinetic data were tested using pseudo first-order and pseudo second-order models. The results suggested that the pseudo second-order model (R~2 > 0.999) was better for the description of the adsorption behavior of zinc(II) onto the dried activated sludge. Fourier transform infrared spectral analysis showed that the dominant mechanism of zinc(II) biosorption onto the dried activated sludge was the bind between amide groups and zinc ions.
生物发光法检测CD4+T细胞内ATP的意义及在器官移植中的应用

细胞免疫(CMI)由T淋巴细胞和它们的效应因子反应,以及与其他免疫细胞的交互作用调节。CMI在器官移植、传染病控制及抗肿瘤等作用中具有重要意义。细胞免疫功能检测方法包括体内检测方法 (如皮试)和体外检测方法(细胞活性信号检测、淋巴细胞增殖试验、细胞毒性试验和细胞因子检测等)。目前研究细胞免疫功能的方法主要是以淋巴细胞为主．淋巴细胞体外增殖试验(LPA)是其传统体外模式。但此种检测方法耗时长．且不能确定是外周血中哪种类型的前体细胞增殖。随着人们对T淋巴细胞研究的逐渐深入．认为代表T细胞活性的细胞内三磷酸腺苷(ATP)含量与其增殖具有相关性。CD4+T辅助细胞是行使细胞免疫功能的关键因素。预测机体细胞免疫状态的CD4+T细胞内ATP水平检测已被临床应用。
系统信息支持对老年冠心病患者疾病不确定感的影响

目的探讨系统信息支持对老年冠心病患者疾病不确定感的影响。方法 将60例老年冠心病患者随机分为实验组和对照组各30例，实验组由实验者提供有针对性的信息 支持；对照组按常规进行健康教育；采用疾病不确定感量表(MUIS)对两组患者于入院后及出院前一天进行疾病不确定感调查。结果 干预前实验组患者和对照组患者疾病不确定感水平差异无统计学意义；实施护理干预后，实验组的疾病不确定感下降幅度显著高于对照组 ( P〈  0.05)。结论 信息支持能明显降低患者的疾病不确定感。
红叶腺柳扦插育苗技术

红叶腺柳为杨柳科柳属树种。由中国林科院经济林研究开发中心侯元凯教授等于2003年秋季在野外考察时发现并命名,因之为腺柳的变种,故命名为红叶腺柳,简称红叶柳。红叶腺柳叶长614 cm,宽37 cm。叶缘有锯齿,叶椭圆形、卵圆形或椭圆状披针形。红叶腺柳顶端新叶45片,于4月下旬至10月上旬呈红色。树冠红、黄、绿相间,随着时间的推移,叶片由鲜红色变为橙黄色,再由橙黄色转变成绿色,叶背呈苍白色。红叶腺柳从观赏性、抗逆性、适应性和繁殖力上看,是目前绿化市场上一个灿烂的新星。
不同播期对春播红小豆产量性状的影响

从5月10日开始播种截止到7月19日,共设计了8个播期。通过两年试验,对不同播期小豆产量性状进行了分析。试验结果表明:除了7月9日和7月19日两个播期不能正常成熟外,其它6个播期均能正常成熟。从不同播期对春播红小豆产量性状的影响方面来看,辽宁省春播小豆的最佳播期为5月20日。
浅析东北春播玉米育种主要目标和提高育种效率的途径

针对限制我国东北玉米产量提高的主要因素,指出了东北玉米育种的3个主要目标,即耐密育种、抗逆育种和抗病虫育种,并提出了提高东北玉米育种效率的3个途径:(1)种质资源扩增、改良与创新; (2)育种方法的改进; (3)栽培技术与玉米育种的有机结合。
拟南芥AtSUC突变体在干旱胁迫下的形态学差异

研究以拟南芥野生型和蔗糖转运蛋白单基因纯合突变体atsuc3、atsuc5、atsuc9为材料,利用不同浓度的聚乙二醇(PEG-6000)模拟干旱胁迫,研究了对其生长发育的影响。试验结果表明: PEG模拟干旱胁迫使野生型和AtSUC突变体抽苔时间、开花时间、果荚个数和种子千粒重,以及水势和相对含水量均发生变化,而且不同突变体之间变化趋势有显著差异,说明该家族中不同成员对在干旱胁迫下的作用不同。
单源最短路径算法的图示教学设计与实践

单源最短路径是GIS网络分析的一个重点内容,对GIS、空间信息技术等相关专业的学生来讲,由于经典的最短路径算法 (Dijkstra)描述较抽象,让学生掌握单源最短路径算法的本质思想较难。提出用图示教学法来教授GIS中单源最短路径算法的基本原理和思路,详细介绍了图示表示的过程,可以为最短路径算法及其应用的教学过程提供参考。
海南大地控制网一致性研究

首先对海南已有的四套不同时期坐标成果进行公共点求取,再由公共点进行方案设计,然后以相似变换、仿射变换、双线性变换和二维七参数变换为转换模型对方案进行转换模型求解,通过计算分析仿射变换的转换效果比使用相似变换和双线性变换效果要好。最后利用Surfer软件和AutoCAD软件对转换后坐标与原始坐标差值生成差值等值图,研究各套坐标成果之间的差值关系。通过研究对旧坐标系下坐标成果的利用提出建议,并编写系统性软件,为2000国家大地坐标系在海南的推广使用打下基础。
高压氧对牙周炎舰员牙龈血流量、袋内厌氧菌和谷胱甘肽过氧化物酶的作用

目的探讨高压氧(HBO)对舰员重症牙周炎牙龈血流量(GBF)、袋内厌氧菌和谷胱甘肽过氧化物酶(GPx)的作用。方法 90例重症牙周炎患者分为3组,每组30例:HBO组、HBO加洁治组[HBO+SCA(scaling)组]和双氧水组(HP组),在治疗前、后即时和治疗结束1个月时用激光多普勒血流仪测定GBF、牙周袋厌氧菌培养、DTNB比色法测定GPx活性,记录牙龈指数(GI)、龈沟出血指数(SBI)、菌斑指数(PLI)、牙周袋深度(PD)、牙周附着丧失(AL)。结果 3组治疗后即时和治疗结束1个月时的GBF、袋内厌氧菌数和GPx与治疗前比较差异均有统计学意义(P< 0.01),HBO组和HBO+SCA组治疗后即时和治疗后1个月时的各指标与HP组比较差异有统计学意义(P< 0.01); HBO+SCA组治疗后即时和治疗后1个月时的各指标与HBO组、HP组比较差异有统计学意义(P< 0.01),HBO和HBO+SCA组治疗后1个月各指标与治疗后即时比较无差异无统计学意义。结论 HBO对舰员牙周炎有明显治疗作用,HBO加洁治更好,并维持1个月以上,其疗效与抑制致病性厌氧菌,增加GBF和GPx活性有关。
儿童难治性肺炎支原体肺炎44例临床分析

目的分析总结难治性肺炎支原体肺炎(RMPP)患儿临床特点。方法收集于首都医科大学附属北京儿童医院2009年10月至2011年10月住院治疗的104例肺炎支原体肺炎(MPP)患儿,其中44例应用大环内酯类药物治疗至少5 d,临床和影像学表现仍有恶化,将其定义为RMPP组,余60例为MPP组。比较两组患儿一般资料、外周血白细胞总数及分类、C反应蛋白(CRP)、乳酸脱氢酶(LDH)、铁蛋白(SF)、血清免疫球蛋白、CD系列、凝血功能、Galectin-7、纤维支气管镜表现、胸部影像学表现等的差别。结果与普通MPP比较,RMPP患儿外周血中性粒细胞及单核细胞绝对值增高,淋巴细胞计数及绝对值降低;外周血CRP、LDH、SF、Ig M、Ig E、Galectin-7明显增高;存在高凝状态;易合并其他感染;支气管镜下RMPP组患儿常有黏液分泌物阻塞,部分出现黏膜糜烂、坏死物阻塞;肺部影像学常提示肺部大片高密度均匀实变影(常超过2/3叶),常伴有胸腔积液。结论RMPP患儿辅助检查有自身特点,提示RMPP发生与过强的免疫炎症反应、黏液高分泌、高凝状态及混合感染等有关。
基于抗原表位分析的人乳头瘤病毒16L1壳蛋白基因克隆及表达纯化

目的:选择人乳头瘤病毒(HPV)16L1抗原性最强的一段抗原表位编码区,构建原核表达载体并利用大肠杆菌表达抗原蛋白,为HPV疫苗的研究及宫颈癌的诊断奠定基础。方法:借助DNAstar软件分析HPV16L1的CDS区,选择抗原性最强的一段抗原表位编码区,提取HPV16L1编码区的DNA。目的DNA和p ET32a质粒分别进行双酶切,连接并转化DH5α大肠杆菌,筛选阳性克隆后转化BL21大肠杆菌,诱导产生HPV16L1重组蛋白,通过市售抗体对重组蛋白的抗原特异性进行鉴定。结果:利用DNAstar软件,选择了抗原表位较富集的碱基序列919~1314bp区段,按常规制备重组蛋白的方法成功制备HPV16L1重组蛋白。抗体鉴定表明,制备的重组蛋白抗原特异性良好。结论:有针对性地制备HPV16L1重组蛋白,可避免其他抗原表位的干扰。经鉴定,HPV16L1重组蛋白抗原特异性良好,将为HPV疫苗的研究及宫颈癌的诊断奠定基础。
Quantitative Analysis of Relationships Between Crack Characteristics and Properties of Soda-saline Soils in Songnen Plain, China

The Songnen Plain has a typical soda-saline soil, which often shrinks and cracks under natural conditions during water evaporation. This study aims to analyze the relationships between the crack characteristics and the soil properties of soda-saline soils quantitatively, and attempts to establish prediction models for the main soil properties of soda-saline soils based on the results. In order to achieve these objectives, a desiccation cracking test was conducted using 17 soil specimens with different salinity levels under controlled laboratory conditions. Correlation analysis was then performed between the crack characteristics and the soil properties. The results indicate that the crack characteristics can well represent the surface appearances of cracked soils, they also can well distinguish the salinity levels of soda-saline soils while the clay contents and mineralogical compositions of soils are stable. Among the crack characteristics, crack length has the best relationships with the salinity levels of soda-saline soils. Specifically, the crack length has high correlation(R~2 > 0.87) with the electrical conductivity(EC), Na~+, CO_3~(2-) and the salinity, it also has reasonable relationship(R~2 > 0.68) with HCO_3~–, this indicates crack length can be well used for the prediction of these properties of soda-saline soils.
足细胞病治疗的新靶点

足细胞损伤是肾小球性蛋白尿发生的主要机制,与多种肾小球疾病的发生发展关系密切。足细胞病是指足细胞结构和功能改变为主要特点的肾小球疾病。目前治疗足细胞病的药物主要为免疫抑制剂,但疗效局限且不良反应显著。近年来研究发现,足细胞病不仅与免疫炎性反应有关,还与循环因子、脂代谢异常关系密切。针对足细胞病发生过程中的关键环节,研发新型药物及诱导足细胞再生可为足细胞病治疗提供新方向。本文着眼于足细胞病及其治疗的新靶点做一综述。
兔实验性骨关节炎中6种血清生物标志物的检测

骨关节炎(osteoarthritis,OA)是一种发病率逐年增高的慢性关节疾病,已成为人类致残的第一大疾病,称为"不死的癌症"。现有的OA诊断方法以临床体征结合影像学检查为基础,不能在关节软骨的组织结构发生不可逆损伤前进行诊断,如能运用有效、廉价和微创的检查手段在有临床症状而未出现影像学改变的人群中筛选出OA早期患者,做到早发现早治疗,就可以延缓甚至逆转疾病的发展,这也是防治OA的重要方法。回顾近年来国内外文献发现IL-1β/COX-2/PGE2/MMP-1/CTX-Ⅱ和IL-1β/NO/MMP-1/CTX-Ⅱ两调控轴在OA的发生发展中有重要作用。本研究通过检测血清中相关因子水平,探讨其在早期OA中的作用。
淫羊藿素固体脂质纳米粒的工艺研究及其表征

目的: 制备淫羊藿素固体脂质纳米粒(ICT-SLN),并考察制备过程中的主要影响因素。方法: 通过试验确定制备工艺参数,采用单因素试验初选ICT-SLN的制备条件,以包封率为评价指标,采用正交试验设计法确定最优处方。结果: 制得的ICT-SLN的平均粒径为47.5 nm,包封率达到76.58%。结论: ICT-SLN的包封率较高,稳定性良好,高压乳匀法适用于ICT-SLN的制备。
催吐萝芙木幼苗生长对温水及外源激素浸种的响应

目的: 了解催吐萝芙木Rauvolfia vomitoria幼苗生长对IBA和GA_3与温水浸种的响应。方法: 采用L_9 (3~4)和L_8 (2~7)的正交设计进行试验。结果: 平均地径、苗高、下胚轴和根长分别为0.59 ~ 0.82 mm、4.43 ~ 7.23 cm、3.43 ~ 5.63 cm和4.05 ~ 6.19 cm,且处理组合间苗高和下胚轴具有极显著的差异。结论: IBA和GA_3是影响催吐萝芙木下胚轴和苗高生长的主导因子,低浓度的IBA(0.25 g /L)和高浓度的GA_3 (1.00 g /L或0.80 g /L)与40 ~ 60 ℃温水浸种的组合可显著地促进催吐萝芙木幼苗生长。
携带腓肠肌的胫后动脉穿支皮瓣修复足踝部腔隙性软组织缺损

目的探讨携带腓肠肌的胫后动脉穿支蒂隐神经营养血管复合组织瓣修复足踝部腔隙性软组织缺损的手术方法及临床效果。方法2011年1月-2014年9月,采用携带腓肠肌的胫后动脉穿支蒂隐神经营养血管复合组织瓣修复足踝部腔隙性软组织缺损患者13例,均合并有皮肤和下肢骨质缺损。彻底清创后行VSD治疗5 ~ 7 d, 1 ~ 3次,每次更换海绵,待感染控制、创面清洁后行皮瓣手术修复创面,皮瓣面积7.5 cm × 6.0 cm ~ 23.0 cm × 11.0 cm,腓肠肌肌瓣面积为4.5 cm × 4.0 cm ~ 11.0 cm × 5 cm。皮瓣供区植皮后覆盖VSD治疗。结果术后13例皮瓣全部成活,2例皮瓣远端部分表皮坏死,换药后愈合。所有病例术后随访6 ~ 15个月,平均8.4个月。皮瓣外形满意,质地柔软,色泽与受区相近,无局限性臃肿,局部未见窦道形成,足部功能恢复良好。术后6个月对随访患者进行感觉功能评定,13例皮瓣均恢复S_2级及以上感觉。皮瓣供区植皮后完全成活。结论携带腓肠肌的胫后动脉穿支蒂隐神经营养血管复合组织瓣无需吻合血管,成活率高,不仅可有效修复足踝部较大面积的软组织缺损,而且能有效填塞死腔,增强局部抗感染能力,方便临床推广应用。
切取阔筋膜的股前外侧皮瓣在足踝皮肤肌腱缺损中的应用

目的总结扩大切取阔筋膜的股前外侧游离皮瓣一期修复足踝皮肤、肌腱缺损的临床应用。方法自2000年10月至2014年6月,应用扩大切取阔筋膜的股前外侧游离皮瓣一次性修复足踝皮肤、肌腱缺损18例,其中外踝皮肤缺损并跟腱缺损4例,内踝皮肤缺损并跟腱缺损3例,足背皮肤缺损并趾长伸肌腱缺损11例,其中1条4例,2条4例,3条1例,4条1例,5条1例。致伤原因:重物砸伤6例,车祸伤8例,高处坠落伤3例,先天性马蹄内翻足畸形应用Ilizarov外固定支架技术矫形,因牵张过快张力过高,导致外踝及足跟外侧皮肤坏死伴跟腱坏死、缺损1例。血管吻合采用端端吻合法。结果有1例皮瓣远端部分坏死,予以换药清创,皮肤减张缝合器逐渐牵张愈合,其余皮瓣全部成活,所有病例随访6 ~ 18个月,修复部位外观功能良好,皮瓣感觉恢复至S_2~S_4级。结论扩大切取阔筋膜的股前外侧游离皮瓣移植是修复足踝皮肤、肌腱缺损的较好方法。
热证诊断标准研究概述

热证是中医辨证分型中的一个重要证型,综合分析近年来有关热证诊断及分级量化的研究显示,虽然目前研究者对热证包括实热、虚热、阴虚热、气血热、血热、痰热、瘀热、毒热、风热、胃、心、肺等脏腑热,以及单病种如冠心病、慢性胃炎、慢性阻塞性肺疾病、糖尿病等热证诊断标准做了大量研究,但目前尚未形成公认规范的热证诊断及量化标准,无法对临床和科研提供有效帮助。鉴于临床诊断和疗效评价对复杂证候所含证素组合分解的需要,推荐以单一证素有序组合为基础的多层级热证诊断标准模式,即热证一级诊断,虚热、实热证二级诊断,实热证和虚热证的基础上加上单一病性证素做出的三级诊断,三级诊断的基础上加上单一病位证素得出的四级诊断。这样的四级诊断体系条理清晰、病性清楚、定位明确,可为热证的分级诊断及量化研究工作提供参考。