mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Effect of electric intensity on the microbial degradation of petroleum pollutants in soil

Electro-bioremediation is an innovative method to remedy organic-polluted soil. However, the principle of electrokinetic technology enhancing the function of microbes, especially the relationship of electric intensity and biodegradation efficiency, is poorly investigated. Petroleum was employed as a target organic pollutant at a level of 50 g/kg (mass of petroleum/mass of dry soil). A direct current power supply was used for tests with a constant direct current electric voltage (1.0 V/cm). The petroleum concentrations Were measured at 3275-3285 nm after extraction using hexane, the group composition of crude oil was analyzed by column chromatography. The water content of soil was kept 25% (m/m). The results indicated the degradation process was divided into two periods: from day 1 to day 40, from day 41 to day 100. The treatment of soil with an appropriate electric field led the bacteria to have a persistent effect in the whole period of 100 days. The highest biodegradation efficiency of 45.5% was obtained after treatment with electric current and bacteria. The electric-bioremediation had a positive effect on alkane degradation. The degradation rate of alkane was 1.6 times higher in the soil exposed to electric current than that treated with bacteria for 100 days. A proper direct current could stimulate the microbial activities and accelerate the biodegradation of petroleum. There was a positive correlation between the electric intensities and the petroleum bioremediation efficiencies with a coefficient of 0.9599
Fabrication of asymmetric poly (m-phenylene isophthalamide) nanofiltration membrane for chromium(VI) removal

The feasibility of employing nanofiltration for the removal of chromium(VI) from wastewater was investigated. Poly (m-phenylene isophthalamide) (PMIA) was used to fabricate asymmetric nanofiltration membrane through the phase-inversion technique. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to characterize the obtained membrane, and the both confirmed a much smoother surface which could reduce membrane fouling. The PMIA membrane showed different rejections to electrolytes in a sequence of Na_2SO_4 > MgSO_4 > NaCl > MgCl_2, which was similar to the sequence of the negatively charged nanofiltration membranes. Separation experiments on chromium(VI) solution were conducted at various operating conditions, such as feed concentration, applied pressure and pH. It is concluded that chromium(VI) could be effectively removed from chromiumcontaining wastewater by the PMIA nanofiltration membranes while maintaining their pollution resistance under alkaline condition
Low concentrations of atrazine, glyphosate, 2, 4-dichlorophenoxyacetic acid, and triadimefon exposures have diverse effects on Xenopus laevis organ morphogenesis

Many chemicals are released into the environment, and chemical contamination has been suggested as a contributing factor to amphibian declines. To add to a growing body of knowledge about the impact of individual chemicals on non-target organisms, we examined the specificity of deformities induced by exposure to four pesticides (atrazine, 2, 4-dichloropheoxyacetic acid (2, 4-D), triadimefon, and glyphosate) in the model amphibian species, Xenopus laevis. We focused on the period of organ morphogenesis, as it is frequently found to be particularly sensitive to chemical exposure yet also commonly overlooked. We found similar levels of intestine malformations and edemas, as well as disruption of skeletal muscle, in atrazine and triadimefon exposed tadpoles. The effects of 2, 4-D were only apparent at the highest concentrations we examined; glyphosate did not induce dramatic malformations at the concentrations tested. While researchers have shown that it is important to understand how chemical mixtures affect non-target organisms, our results suggest that it is first crucial to determine how these chemicals act independently in order to be able to identify consequences of individual pesticide exposure
血清HDL-C表面活性剂匀相抑制方法建立

实现HDL-C的匀相直接测定, 必须预先将血清中的游离胆固醇清除, 否则该部分胆固醇将参与HDL -C的显色反应, 使测定结果偏高. 本法将胆固醇氧化酶、过氧化物酶及4-氨基安替比林3种组分设置在试剂1(R_1)中, 将胆固醇酯酶、HDAOS设置在试剂2(R_2)中. 实验结果表明, 3μL血清标本和300 μL R_1.混合并在37℃反应5min后, 有 12 mmol/L的游离胆固醇可以迅速被清除
Debrominated and methoxylated polybrominated diphenyl ether metabolites in rainbow trout (Oncorhynchus mykiss) after exposure to decabromodiphenyl ether

Decabromodiphenyl ether (BDE209) is the primary component in a commonly used flame retardant. Previous studies had proved that BDE209 itself was not toxic, while its metabolites including debrominated diphenyl ethers (De-BDEs) and methoxylated brominated diphenyl ethers (MeO-BDEs) posed a potential threat to organisms. Many studies had indicated that BDE209 could metabolize quickly in mammals, but lacking in the basic data about the metabolism of BDE209 in fish. In the present study, two replicate treatment groups of rainbow trout (Oncorhynchus mykiss) were exposed to BDE209 via a single intraperitoneal injection approximately 100 and 500 ng/g, respectively. Muscle, liver and blood samples were collected to analyze the specific metabolites on day 1 and day 28 post injection. The highest concentration of BDE209 was detected in muscle tissues, from 796.1 ng/g wet weight (day 1) to 687.1 ng/g wet weight (day 28) in high dose group, suggesting that BDE209 could accumulate slightly in muscle tissues. However, BDE209 was not detected in the blood for all treatments. Most congeners of De-BDEs were found in muscle and liver tissues, with the highest concentration in the liver. The main De-BDEs were nona-, octa-, hepta- and penta-De-BDEs. A total of seven MeO-BDE metabolites were observed among different fish tissues. Blood had the highest contribution of the MeO-BDE metabolites. Each MeO-BDE congener increased over the 28 days. These results in contrast to other studies suggested possible species-specific differences in metabolic abilities
Chemical composition and Zn bioavailability of the soil solution extracted from Zn amended variable charge soils

A study on variable charge soils (volcanic Italian and podzolic Scottish soils) was performed to investigate the influence of soil properties on the chemical composition of soil solution. Zinc speciation, bioavailability and toxicity in the soil solution were examined. The soils were spiked with increasing amounts of Zn (0, 100, 200, 400 and 1000 mg/kg) and the soil solutions were extracted using rhizon soil moisture samplers. The pH, total organic carbon (TOC), base cations, anions, total Zn and free Zn~(2+) in soil solution were analysed. A rapid bioassay with the luminescent bacterium Escherichia coli HB101 pUCD607 was performed to assess Zn toxicity. The influence of soil type and Zn treatments on the chemical composition of soil solution and on Zn toxicity was considered and discussed. Different trends of total and free Zn concentrations, base cations desorption and luminescence of E. coli HB101 pUCD607 were observed. The soil solution extracted from the volcanic soils had very low total and free Zn concentrations and showed specific Zn~(2+)/Ca~(2+) exchange. The soil solution from the podzolic soil had much higher total and free Zn concentrations and showed no evidence of specific Zn~(2+)/Ca~(2+) exchange. In comparison with the subalkaline volcanic soils, the acidic podzol showed enhanced levels of toxic free Zn~(2+) and consequently stronger effects on E. coli viability
Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater (MGW) and potato wastewater (PW) as culture medium. The maximum yield of 6.5 x 109 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28℃, pH 6.0-7.5 using the mixture of MGW and PW (1:9)
人工蛹虫草子实体醇提物遗传毒性分析

遗传毒性试验能检出DNA损伤及其损伤的固定, 以基因突变、较大范围染色体损伤、重组和染色体数目改变形式出现的DNA损伤, 一般被认为是遗传效应的基础, 故遗传毒性试验主要用于致癌性预测~[2-3]. 多倍体在体外染色体畸变试验常见, 如果在体外试验中可见多倍体, 而非结构上的染色体断裂, 体内微核试验阴性, 通常表明缺乏诱导非整倍体证据~[4-6]. 由于遗传毒性试验容易出现假阳性和假阴性结果, 因此对结果进行综合分析尤为重要, 如果体外与体内试验的结果不一致, 应进一步分析原因, 如化合物在体内可能发生的代谢、分布、排泄差异等等. 本研究采取体内与体外相结合的研究方法, 避免了单一结果所产生的偏差. 结果表明人工蛹虫草子实体醇提物无致突变作用及遗传毒性
Synthesis and measurement of structural and magnetic properties of K-doped LaCoO_3 perovskite materials

Polycrystalline La_(1-x)K_xCoO_3 (0 ≤ x ≤ 0.2) rare earth cobaltates have been synthesized by a solution combustion method using glycine as a fuel. The synthesized ceramic materials were characterized by powder X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and magnetic measurements and studied for physical properties, such as photocatalytic activity. FTIR measurements in conjunction with XRD showed that phases beyond 10% K doping are accompanied by small amounts of impurities. Chemical titrations show the presence of Co~(4+) and account for the Co~(3+)Co~(4+) mixed-valency of the system. The parent LaCoO_3 shows spin-glass transition at low temperatures, whereas doped samples show transition from spin-glass behavior to paramagnetic ordering on progressive doping of K. "Mixed-conductor" nature of these ceramics positions them as viable candidates for solid oxide fuel cell (SOFC) applications
Design of Robot Welding Seam Tracking System with Structured Light Vision

Robot welding is an important developing direction of welding automation and intelligentization, and automatic seam tracking technology is one of principal research domains. Nowadays, seam tracking system with structured light vision becomes a hot research. Structured light vision seam tracking products abroad are generally very expensive and can only be applied on special occasions. In China, the research of structured light vision seam tracking system is still just on the stage of experiments. A robot real-time seam tracking system with line structured light vision is designed. The hardware system is set up, a filtering method for line structure seam image is improved, and compared with common filtering, it has better effect and characteristic of real time. Two methods, fast template matching and fast Hough transform, to recognize the image coordinates of seam center are improved. Two new image recognition methods, structure element matching and corner detecting, are proposed. The comparison of seam image recognition shows that fast template matching and corner detecting are more precise and stable than the other two methods, and corner detecting is the best in real time. A simultaneous calibration for camera parameters and robot hand-eye is also proposed, and calculation shows that the calibration is effective and feasible. The robot seam tracking tests for linear and folded lap-joint are performed, which are based on the above four image recognition methods, and the results indicate that four image recognition methods are all applicable to real-time seam tracking, and the whole system sufficed for the requirements of real-time seam tracking. Automatic seam tracking with line structured light vision is feasible and has good versatility
SYNTHESIS OF HYDROXYL-TERMINATED POLYBUTADIENE POSSESSING HIGH CONTENT OF 1, 4-UNITS VIA ANIONIC POLYMERIZATION

The hydroxyl-terminated polybutadiene (HTPB) possessing high content of 1, 4-units was synthesized by anionic polymerization of butadiene, using alkyllithium containing silicon-protected hydroxyl group as initiator and cyclohexane as solvent. The polymers were characterized by GPC, IR and ~1H-NMR. The mechanical properties of cured films were also evaluated. The results show that the content of 1, 4-units for HTPBs made by anionic polymerization reaches up to 90%. The molecular weight distribution is very narrow (≤ 1.05). The functionality of hydroxyl groups approaches 2. Compared with free radical HTPB, the elongation at break of anionic HTPB films increased by 70%, while the tensile strength remained nearly unchanged. This new HTPB can be very useful in solid propellant
立体定向半月神经节脉冲射频热凝术治疗原发性三叉神经痛

原发性TN的治疗方法众多, 经皮穿刺选择性三叉神经半月神经节温控脉冲射频热凝术操作简便、安全有效、严重并发症发生率和病死率较低、费用低廉. 1931年Kirschner介绍了电凝术治疗TN, 但是直到1974年Sweet和Wepsic对射频热凝术在设备和技术上进行了一系列改进之后, 射频热凝术才成为治疗TN的主要手段之一. 据统计, 该治疗方法平均有效率约96%~[2]. 为减少严重的眼部并发症的发生, 本研究未选择累及三叉神经第1支的患者
Study of Thermodynamics of Liquid Noble-Metals Alloys Through a Pseudopotential Theory

The Gibbs-Bogoliubov (GB) inequality is applied to investigate the thermodynamic properties of some equiatomic noble metal alloys in liquid phase such as Au-Cu, Ag-Cu, and Ag-Au using well recognized pseudopotential formalism. For description of the structure, well known Percus-Yevick (PY) hard sphere model is used as a reference system. By applying a variation method the best hard core diameters have been found which correspond to minimum free energy. With this procedure the thermodynamic properties such as entropy and heat of mixing have been computed. The influence of local field correction function viz; Hartree (H), Taylor (T), Ichimaru-Utsumi (IU), Farid et al. (F), and Sarkar et al. (S) is also investigated. The computed results of the excess entropy compares favourably in the case of liquid alloys while the agreement with experiment is poor in the case of heats of mixing. This may be due to the sensitivity of the heats of mixing with the potential parameters and the dielectric function
Fluctuation Analysis of Decoy State QKD with Finite Data-Set Size

Decoy state method quantum key distribution (QKD) is one of the promising practical solutions for BB84 QKD with coherent light pulses. The number of data-set size in practical QKD protocol is always finite, which will cause statistical fluctuations. In this paper, we apply absolutely statistical fluctuation to amend the yield and error rate of the quantum state. The relationship between exchanged number of quantum signals and key generation rate is analyzed in our simulation, which offers a useful reference for experiment
Multiformity of Optical Quantum Coherence Systems with Y and Inverted Y-type Schemes

Y and inverted Y-type four-level schemes for optical quantum coherence systems, which may be intuitively considered to be very simple, have not been studied intensively till now. In this paper, we present the multiformity of these two types of schemes by considering that they can be classified into nine possible level styles as the second-order sub-schemes using laser fields. Further we point out the complexity of their more than one hundred realistic configurations as the third-order four-level sub-schemes that may appear in the optical quantum coherence experiments. Throughout this paper we review which configurations have been studied in some research aspects and which ones not, according to our knowledge, in order to be propitious to next steps of theoretical and experimental investigations, especially for applications in the fields of quantum optics, quantum information science, laser spectroscopy, and so on
羊开口药材的名实考证

目的对民间常用中药材羊开口原植物来源进行考证.方法考查本草文献中羊开口原植物和药用部位的记载及附图, 野外调查羊开口药材原植物的来源, 通过产地药农收集羊开口原植物标本, 核对原植物标本并咨询分类专家.结果 目前市场上羊开口原植物来源是野牡丹科展毛野牡丹和野牡丹的根, 部分地区同时混杂多花野牡丹和毛稔的根.结论为更好地制定羊开口新的药材质量标准, 并为羊开口药材收购提供依据, 保证羊开口药材的质量
巴戟天多糖对骨质疏松大鼠骨密度及血清微量元素的影响

目的探讨巴戟天多糖对切除卵巢后骨质疏松大鼠骨密度及血清微量元素的影响.方法切除大鼠双侧卵巢, 建立骨质疏松模型, 造模后分别给予巴戟天多糖(100、300 mg/kg)干预, 给药30 d后检测大鼠骨密度及骨矿物质、血清IL-6和TNF-α水平、血清微量元素水平.结果 巴戟天多糖组大鼠骨质密度及骨矿物质水平高于模型组, 血清IL-6、TNF-α表达水平低于模型组, 骨矿物质及血清微量元素水平高于模型组, 并且高剂量作用显著.结论巴戟天多糖能够提高切除卵巢后骨质疏松大鼠骨密度, 其可能通过调节血清微量元素水平及IL-6、TNF-α表达水平发挥作用
黑龙江地区温室生产中沼气综合利用初探

沼气是一种具有较高热值的可燃气体, 可替代部分石油、煤炭等化石燃料, 与其它燃气相比, 其抗爆性能较好, 是一种很好的清洁燃料, 传统上大多利用沼气进行取暖、炊事和照明.随着对环境的日益重视, 人们开始利用各种方式来减少工农业生产对环境的破坏.近十几年来, 在各级政府有关部门和企业的帮助协调下, 用于处理畜禽粪便及各种生产、生活污水的大中型沼气工程纷纷上马.黑龙江地区温室生产中沼气的综合利用也逐渐发展起来
凡河都市农业区功能定位与规划研究

利用SWOT分析方法, 对辽宁省铁岭市凡河都市农业区的发展条件进行了综合分析, 探讨了该区的经济、生态、休闲、文教、示范等功能, 并从空间布局、特色产业、设施农业、新农村建设、配套服务等方面提出了规划思路
陕南农村养蚕劳动力结构变化与产业政策导向分析

在查阅相关文献的基础上, 应用普查与抽样观察法分析陕西南部农村劳动力结构变化.结果表明, 当前农村养蚕劳动力结构受农村劳动力资源与外出从业劳动力结构的共同影响, 呈现出数量缺乏、结构老化、男性化、质量下降的特征.建议利用产业政策的导向作用, 调整蚕桑生产新技术的研发方向, 改变现行养蚕技术体系和推广示范方法, 适应农村养蚕劳动力结构变化的现实
当前我国农民收入现状与增收对策分析

随着我国现代化进程的加快, 如何解决农民收入的持续增长问题, 是保证新农村建设目标实现的一个重要课题.当前, 我国农民自身素质差, 政府的投入和支持力度不够, 工农产品价格仍然存在的"剪刀差"以及小农生产和大市场之间存在矛盾等问题制约着农民增收.增加农民收入, 必须重视农民教育, 推进农业现代化和信息化建设, 加强政府对农业的投资和支持力度, 推进农民收入向多元化转变
农民经济合作组织的非经济作用及价值

农民经济合作组织具有多方面的非经济发展的功能作用:巩固党在农村的执政基Ⅺ出的政治作用;有助于改变我国农民"袋装的马铃薯"的低组织化现状;有力地促进公平正义的公共服务功能;增强社会认同感和培育农村信任文化;成为弱势群体的农户参与公共事务的组织载体;是构成中国特色社会主义文化和社会主义核心价值体系建设的重要思想资源
基于权的最小平方法的农地产权制度绩效评价----以重庆市璧山县大路镇为例

采用权的最小平方法, 从激励约束功能、资源配置功能、交易功能方面构建评价指标体系对重庆市璧山县大路镇农地产权制度绩效进行定量评价.结果表明, 该镇农地产权制度绩效的综合评价系数为0.663 9, 处于中度效率水平.对大路镇农地产权制度绩效贡献大小依次为激励约束功能>资源配置功能>交易功能.农地产权改革应着重发挥对农地产权制度的激励约束功能, 提高耕地垦殖率, 遏制耕地撂荒, 提高农业投入, 严格控制违法"农转非"
紫叶李纯林空气负离子浓度的研究

采用空气负离子测量仪, 对上海市松江区中央公园的紫叶李纯林绿地的空气负离子浓度进行了测定, 并用相关方法进行评价.结果表明, 紫叶李纯林在5月、7月和9月的空气负离子浓度日变化曲线呈双峰型;6月和8月的日变化曲线相似, 几乎平行.相关性分析表明, 紫叶李纯林内空气负离子浓度与相对湿度呈正相关关系'与温度呈负相关关系
109国道永宁段公路两侧土壤中铅和镉的污染评价

以109国道永宁段两侧土壤为研究对象, 分析了公路两侧土壤中铅、镉的含量, 并对铅、镉的污染程度进行了评价.研究结果表明:109国道永宁段公路两侧土壤中的铅、镉含量均超过土壤背景值, 且含量随着与公路距离的增加而逐渐减少;按照地积累指数评价方法进行污染程度评价, 发现距离公路100 m范围内, 土壤铅处于轻度至中度污染, 土壤镉虽处在无污染水平, 但仍超过土壤背景值, 在以后的生产中应加强管理
江苏省秸秆资源利用现状及多层次利用模式探讨

农作物秸秆是一种宝贵的生物质资源, 如何推进秸秆的开发利用, 保护生态农业的发展, 是一个重要的课题.文章分析了江苏省秸秆利用的现状和存在的问题, 指出秸秆循环利用的必要性及需要贯彻的原则, 探讨了秸秆多层次循环利用模式, 有针对性地提出建议和解决问题的具体措施, 预测了秸秆利用技术产业化发展和多层次利用的前景
芦苇和香蒲对富营养化水体的净化效果

用泡沫塑料板作为漂浮载体, 研究单独和等量混合种植芦苇和香蒲(每缸10株)对富营养化水体的净化效果.结果表明:芦苇和香蒲在供试富营养化水体中均能正常生长, 二者单独种植或等量混合种植对富营养化水体均有一定的净化能力.芦苇单独种植和香蒲单独种植及等量混合种植50 d时, 对供试水体中总氮的去除率分别为 91.90%、92.30%和94.56%;对铵态氮的去除率分别为90.27%、92.240/0和91.11%;对总磷的去除率分别为 84.85 010、72.73%和75.76%;对COD的去除率分别为82.6%、83.0%和83.7%.同时还对几种植物种植方式引起水体总氮、铵态氮、总磷浓度下降与时间的关系作回归分析, 结果表明, 随着处理时间的延长, 水体中总氮、铵态氮、总磷浓度呈负指数形式衰减.各项指标分析表明, 处理富营养化水体中总氮、铵态氮、总磷、COD效果最好的处理分别为混合种植、香蒲单植、芦苇单植、混合种植
大叶白麻黄酮类化学成分研究

采用溶剂萃取及柱色谱等方法从大叶白麻叶中分离得到3种化合物, 采用理化常数、光谱分析、标准品对照等方法进行分析鉴定.结果表明, 3个化合物分别为金丝桃苷、芸香苷、槲皮素
含水率对西瓜籽性能影响的试验研究

含水率是影响西瓜籽性能的重要参数, 通过试验获得在不同含水率(0. 50%、3.80%、8.79%、13.54%、 19.90%、29.60%、41.71%)下西瓜籽的破壳力与压缩变形的关系曲线, 并分析不同含水率对西瓜籽各性能参数的影响.结果表明:西瓜籽的临界破壳力、临界压缩变形、抗压强度、单位变形、破壳能随着含水率的增加先升后降, 西瓜籽的弹性模量随含水率的增加先降后升
鹅掌柴中总黄酮含量的测定

采用50%稀乙醇冷浸法、70%乙醇热回流法和95%乙醇超声波提取法提取鹅掌柴中总黄酮, 以芸香苷为对照品, 在A =510 nm处分别测定鹅掌柴根、茎、叶中总黄酮类化合物的含量.结果表明, 芸香苷在0-0.05 mg/mL范围内峰吸光度与浓度线性关系良好(r =0.999 9, n=6), 仪器精密度RSD =0.31%(n=6).50%稀乙醇冷浸法中鹅掌柴根、茎、叶总黄酮含量分别为3.54%、2.01%、6.11%;70%乙醇热回流法中鹅掌柴根、茎、叶总黄酮含量分别为 4.48%、3.81%、7.14%;95%乙醇超声波提取法中鹅掌柴根、茎、叶中总黄酮含量分别为4.60%、3.95%、7.26%
苏钟杂优母猪人工授精效果初步分析

利用人工授精和本交2种配种方法对苏钟杂优母猪繁殖性能的表现进行初步观察与分析.结果表明:人.工授精的苏钟杂优初产母猪窝平均总产仔10.22头, 平均产活仔8.17头, 仔猪平均初生重1.41 kg, 仔猪28日龄平均断奶重6.41 kg;本交的苏钟杂优经产母猪窝平均总产仔12. 22头, 平均产活仔数10.39头, 仔猪平均初生重1.34 kg, 仔猪28日龄平均断奶重6.33 kg.苏钟杂优后备母猪在适配龄时进行人工授精后, 其繁殖性能的总体表现比较好, 在试验的预期范围内
遮阳网覆盖对初夏湿栽水芹产量及品质的影响

在晚春采用单层遮阳网覆盖栽培湿栽水芹试验的基础上, 进一步于初夏开展多层遮阳网覆盖栽培试验, 比较了水芹产量、小叶总黄酮含量、叶柄粗纤维和总酚含量及DPPH自由基清除率等指标.结果表明, 随覆盖层数增加, 水芹产量显著下降, 多项品质指标也有不同程度变化
形态学性状及AFLP标记在木瓜海棠新种质鉴别和分类中的应用

木瓜属是我国传统观赏花木和中药材, 目前已发表的5个原种中4种原产中国, 我国拥有绝对的资源优势, 因此, 利用我国木瓜海棠巨大的资源优势, 进行种质资源创新, 对丰富木瓜海棠新品种和园林应用前景具有重要意义.对上海植物园杂交选育的20个优良单株进行形态学性状和AFLP标记2个层面的分类和遗传鉴别.结果表明:从64对引物组合中筛选出9对引物组合效果较好, 共扩增出1 367条清晰条带, 其中共有位点40个, 多态性位点 1327个, 多态性97.07%.9对引物能够很好地区分不同的杂交后代, 但采用AFLP标记对杂交后代进行分类的结果与形态学方法存在一定差异
新型矿物肥料在甘蔗上的应用试验

针对红壤土土壤营养组分贫缺, 易于板结和保肥水性能差的特点, 研究了新型矿物肥料, 对种植于区内的糖蔗进行了田间试验, 以探究新型肥料的综合效能.试验表明:在甘蔗施放常规肥料基础上, 蔗地增施矿物肥料 300~1050 kg/hm~2, 能促进甘蔗的生长发育, 提高产量和品质, 取得了较好的试验效果
不同土壤基质中水花生生长和荧光响应差异

采用盆栽法, 研究入侵植物水花生在7种不同土壤基质中的生长和荧光响应差异.结果表明:(1)不同土壤基质对水花生的形态特征产生了显著影响, 当水花生生长在泥炭、菜园土和水稻土中时, 其叶片数目、分株数、植株总长度、叶面积和分枝数量等指标会显著高于其他土壤基质处理;(2)生长在河沙和红壤中的水花生生物量最小, 而生长在泥炭中的最大;(3)生长在河沙中的水花生根冠比最大, 生长在泥炭和红壤中的其次;(4)生长在河沙和红壤中的水花生叶片F_v/F_m最小, 植株的光合活性最弱.研究结果表明, 不同土壤类型显著影响水花生的生长表现, 但水花生可以通过选择不同的适应对策应对环境压力, 从而保持其强大的入侵能力
正交设计优化广藿香基因组SRAP扩增体系的研究

以改良CTAB法提取的广藿香叶片DNA为模板, 采用正交试验设计, 从Mg~(2+)、dNTP、引物浓度和DNA聚合酶4因素3水平, 对SRAP扩增效果进行研究, 比较不同模板DNA浓度对PCR扩增的影响, 确立适合广藿香SRAP-- PCR反应的最佳体系.利用SRAP-PCR优化体系对引物进行了全面筛选.结果表明:各因素不同水平浓度对PCR反应结果均有显著影响, 总体积20μL的SRAP - PCR优化反应体系中含有:2μL10xbuffer、20 ng模板DNA、 1.5 mmol/L Mg~(2+)、250μmol/L dNTP、0.3μmol/L Primer、Taq DNA聚合酶1.5 U.运用该体系对部SRAP香单株进行检验, 证明该体系稳定可靠;以此体系为基础从90对引物中共筛选出扩增条带清晰、多态性丰富的sRAP引物18对
核盘菌多聚半乳糖醛酸酶基因克隆及诱饵蛋白表达载体构建

真菌多聚半乳糖醛酸酶抑制蛋白(PGIP)能够特异性识别真菌分泌的降解植物细胞壁的主要物质多聚半乳糖醛酸酶(PG)并抑制其活性.目前, 酵母双杂交技术是快速、有效获取植物PGIP的主要途径, 而其前提是要获得真菌PG的酵母诱饵载体.本研究以核盘菌的PG cDNA为模板, 经PCR扩增获得1 125 bp片断, 进一步将其克隆到 pEASY - Tl载体上.以克隆了目的基因的pEASY - Tl质粒为模板, 用含Nde I和Sal I位点的引物对PG-F_2/PG-R_2扩增获得1125 bp大小的目的片段, 将其连接到pEASY - Tl上生成pEASY - PG.用Nde I和Sal I双酶切pEASY -PG, 回收1125 bp片段并连接到诱饵载体pGBKT7相应的酶切位点上, 转化大肠杆菌菌株DH5a.在含有卡那青霉素的LB平板上筛选获得重组质粒.经Nde I和Sal I双酶切后获得预期的1 125 bp DNA片段, 表明诱饵蛋白载体 pGBKT7 - PG6构建成功.应用醋酸锂介导法将pGBKT7 - PG6转化酵母菌株Y187, 经激活培养基上培养检测, 未发现蓝色菌落, 表明酵母转化子自身不具有自激活功能, 可用于大规模筛选经核盘菌诱导的cDNA表达文库
MATHEMATICAL PROGRAMS WITH SYSTEM OF GENERALIZED VECTOR QUASI-EQUILIBRIUM CONSTRAINTS IN FC-SPACES

In this article, four new classes of systems of generalized vector quasi-equilibrium problems are introduced and studied in FC-spaces without convexity structure. The notions of C_i(x)-FC-partially diagonally quasiconvex, C_i (x)-FC-quasiconvex, and C_i(x)-FCquasiconvex-like for set-valued mappings are also introduced in FC-spaces. By applying these notions and a maximal element theorem, the nonemptyness and compactness of solution sets for four classes of systems of generalized vector quasi-equilibrium problems are proved in noncompact FC-spaces. As applications, some new existence theorems of solutions for mathematical programs with system of generalized vector quasi-equilibrium constraints are obtained in FC-spaces. These results improve and generalize some recent known results in literature
A long-term annual water balance analysis of the Araauai River Basin, Brazil

Semi-arid and arid areas exhibit great temporal variability in water availability. In some of these regions, a one- or two-day rainfall is followed by intervening dry periods of variable length. In recent decades, many rivers of the semi-arid portion of the Jequitinhonha Basin have been undergoing a progressive discharge reduction, mainly of their base flow, the Aracuai River is one of them. In order to understand this transformation, a long-term analysis of the annual water balance of the Aracuai River Basin was performed, the results of which are presented herein. Satellite images, hydrometeorological and river discharge data were analyzed; mean and variance tests were conducted to determine temporal homogeneity. Historical pluviometric data analysis shows no corresponding precipitation reduction and temperature undergoes only a slight increase in the same period. On the other hand, evaporation is extremely high, higher than precipitation during most of the year, leaving almost no water for infiltration (aquifer recharge) and runoff. Furthermore, the Aracuai headwaters of its tributaries are now occupied by a monoculture, Eucaliptus sp., used for paper production. Because of the decreased fluvial discharges, its lowlands, usually used for agriculture and pasture, are abandoned and partially eroded