mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
GLP inhibits heterochromatin clustering and myogenic differentiation by repressing MeCP2

Myogenic differentiation is accompanied by alterations in the chromatin states, which permit or restrict the transcriptional machinery and thus impact distinctive gene expression profiles. The mechanisms by which higher-order chromatin remodeling is associated with gene activation and silencing during differentiation is not fully understood. In this study, we provide evidence that the euchromatic lysine methyltransferase GLP regulates heterochromatin organization and myogenic differentiation. Interestingly, GLP represses expression of the methyl-binding protein MeCP2 that induces heterochromatin clustering during differentiation. Consequently, MeCP2 and HP1γ localization at major satellites are altered upon modulation of GLP expression. In GLP knockdown cells, depletion of MeCP2 restored both chromatin organization and myogenic differentiation. These results identify a novel regulatory axis between a histone methylation writer and DNA methylation reader, which is important for heterochromatin organization during differentiation.
PHF20 collaborates with PARP1 to promote stemness and aggressiveness of neuroblastoma cells through activation of SOX2 and OCT4

The differentiation status of neuroblastoma (NB) strongly correlates with its clinical outcomes; however, the molecular mechanisms driving maintenance of stemness and differentiation remain poorly understood. Here, we show that plant homeodomain finger-containing protein 20 (PHF20) functions as a critical epigenetic regulator in sustaining stem cell-like phenotype of NB by using CRISPR/Cas9-based targeted knockout (KO) for high-throughput screening of gene function in NB cell differentiation. The expression of PHF20 in NB was significantly associated with high aggressiveness of the tumor and poor outcomes for NB patients. Deletion of PHF20 inhibited NB cell proliferation, invasive migration, and stem cell-like traits. Mechanistically, PHF20 interacts with poly(ADP-ribose) polymerase 1 (PARP1) and directly binds to promoter regions of octamer-binding transcription factor 4 (OCT4) and sex determining region Y-box 2 (SOX2) to modulate a histone mark associated with active transcription, trimethylation of lysine 4 on histone H3 protein subunit (H3K4me3). Overexpression of OCT4 and SOX2 restored growth and progression of PHF20 KO tumor cells. Consistently, OCT4 and SOX2 protein levels in clinical NB specimens were positively correlated with PHF20 expression. Our results establish PHF20 as a key driver of NB stem cell-like properties and aggressive behaviors, with implications for prognosis and therapy.
LincRNA-1614 coordinates Sox2/PRC2-mediated repression of developmental genes in pluripotency maintenance

Large-intergenic noncoding RNAs (lincRNAs) cooperate with core transcription factors to coordinate the pluripotency network of embryonic stem cells. The mechanisms by which lincRNAs affect chromatin structure and gene transcription remain mostly unknown. Here, we identified that a lincRNA (linc1614), occupied by pluripotency factors at its promoter, was indispensable for both maintenance and acquisition of pluripotency. Linc1614 served as a specific partner of core factor Sox2 in maintaining pluripotency, primarily by mediating the function of Sox2 in the repression of developmental genes. Moreover, Ezh2, an essential subunit of polycomb repressive complex 2 (PRC2), physically interacted with linc1614 and contributed to lincRNA-mediated transcriptional silencing. Thus, we propose that the interplay of linc1614 with Sox2 implicates this lincRNA as a recruitment platform that mediates transcriptional silencing by guiding the PRC2 complex to the loci of developmental genes.
Diversity and biogeography of a species-rich ant fauna of the Australian seasonal tropics

Although ants are an ecologically dominant and extensively studied faunal group throughout the tropics, there is a poor understanding of tropical ant diversity and distribution at large spatial scales. Here we use a collection developed from 3 decades of ant surveys to present the first analysis of ant diversity and biogeography of a large tropical region. Our objective was to document the species richness, composition, and biogeographic distributions of the ant fauna of the 400 000 km~2 "Top End" of Australia's Northern Territory. The known Top End ant fauna comprises 901 native species from 59 genera. The richest genera are Pheidole (90 species), Melophorus (83),Monomorium (83), Camponotus (71), Meranoplus (63),Polyrhachis (57), Rhytidoponera (50),Tetramorium (43),Cerapachys (32),and Iridomyrmex (31). The fauna is the center of diverse radiations within species-groups of genera such as Meranoplus, Rhytidoponera, and Leptogenys. It also includes IndoMalayan species that have likely bypassed the normal dispersal route into Australia through Cape York Peninsula in North Queensland. Faunistic similarity with other regions of far northern Australia is associated more with rainfall than with geographic proximity. Most (60%) of Top End ant species have not been recorded elsewhere, and, despite uncertainties relating to species delimitation and sampling intensity, this appears to be a credible estimate of the level of endemism. Such exceptionally high endemism can be attributed to the Top End's geographic isolation from other regions of northern Australia with comparably high rainfall.
MiR-223 promotes the doxorubicin resistance of colorectal cancer cells via regulating epithelial-mesenchymal transition by targeting FBXW7

Although doxorubicin has become a key drug in cancer treatment, the resistance of colorectal carcinoma to doxorubicin is a major problem in clinical practice. F-box and WD repeat domain-containing 7 (FBXW7) plays important roles in human cancers and is one of the major causes of drug resistance. The miR-223/FBXW7 pathway has been reported to be a crucial clue to the mechanism of chemoresistance in many human cancers, such as gastric cancer, breast cancer, and non-small cell lung cancer. However, it is unclear whether similar mechanisms of doxorubicin resistance are involved in colorectal cancer (CRC). The aim of the current study was to evaluate the role of miR-223/FBXW7 pathway in chemosensitivity in different CRC cell lines and to investigate the relevant underlying mechanisms. We found that high levels of FBXW7 expression were associated with increased doxorubicin sensitivity in different CRC cell lines, and FBXW7 was regulated by miR-223. Overexpression of miR-223 decreased FBXW7 expression and the sensitivity of CRC cells to doxorubicin, while suppression of miR-223 had the opposite effect. Moreover, epithelial-mesenchymal transition (EMT) was proved to be regulated by miR-223/FBXW7 pathway and involved in the drug resistance. In conclusion, miR-223/FBXW7 axis regulates doxorubicin sensitivity through EMT in CRC, which may lead to the development of individualized treatment in clinical practice.
On Quasi-Chebyshevity Subsets of Unital Banach Algebras

In this paper, first, we consider closed convex and bounded subsets of infinite-dimensional unital Banach algebras and show with regard to the general conditions that these sets are not quasi-Chebyshev and pseudo-Chebyshev. Examples of those algebras are given including the algebras of continuous functions on compact sets. We also see some results in ${{\rm{C}}^ * }$-algebras and Hilbert ${{\rm{C}}^ * }$-modules. Next, by considering some conditions, we study Chebyshev of subalgebras in ${{\rm{C}}^ * }$-algebras.
Associations between serum relaxin 2,aneurysm formation/size and severity of atherosclerosis: a preliminary prospective analysis

Serum relaxin 2 (RL2) is a pleiotropic hormone that acts on various organs and systems, particularly the cardiovascular system. Although RL2 seems to upregulate the synthesis of nitric monoxide (NO) and matrix metalloproteinase (MMP)-2 and -9,current literature on its role in atherosclerosis and aneurysm formation is scarce. The aim of this study was to investigate the levels of serum RL2 in patients with an arterial aneurysm as well as in atherosclerotic patients, and correlate them with the severity of their related vascular disease. A total of 53 subjects were enrolled in this study: 37 patients were scheduled to undergo surgery: 21 patients for different forms of atherosclerotic disease (ATH),16 patients for an arterial aneurysm (AA), 6 patients for undergoing temporal artery biopsy (TAB), and 10 healthy blood donors (HBD) served as the control groups. RL2 was measured using enzymelinked immunosorbent assay. RL2 was significantly higher in AA patients compared to ATH (P<0.01), TAB (P<0.001) and HBD (P<0.01). No significant difference was found between the ATH and TAB groups (P>0.05). In addition, ATH and AA patients were further subdivided based on the severity of their disease. Serum RL2 was progressively increased in patients with arterial aneurysms, showing a positive relationship with the size of the aneurysmatic dilatation. By contrast, the RL2 level was inversely related to the severity of the atherosclerotic disease. Studies with a larger cohort incorporating a consistent study population are warranted to verify our results and shed light on the mechanistic background of these processes.
指侧方岛状皮瓣修复邻指掌侧皮肤软组织缺损并重建血运及感觉

目的探讨指侧方岛状皮瓣修复邻指掌侧皮肤软组织缺损并重建血运及感觉的临床效果。方法2014年6月—2016年2月,笔者单位收治11例手指掌侧皮肤软组织缺损伴指固有神经及指固有动脉缺损患者,创面面积为2.8 cm ×2.5 cm~3.5 cm × 3.0 cm。术前使用多普勒超声血流探测仪探测患指相邻手指桡侧指固有动脉走行。根据创面大小及形状,于患指尺侧相邻手指设计指侧方岛状皮瓣。皮瓣面积较创面面积扩大10%,为3.0 cm×2.7cm~3.8 cm×3.5cm。锐性分离创面远端的指固有动脉及指固有神经,皮瓣远端携带的指固有动脉与创面远端尺侧指固有动脉吻合,皮瓣携带的指固有神经背侧支与创面远端指固有神经吻合。供瓣区取上臂内侧中厚皮修复。结果本组患者术后皮瓣全部成活,皮瓣外观良好,患指末梢血运正常。术后随访7~18个月,平均12个月,皮瓣外观、质地、感觉良好,手指末梢温度、颜色与正常手指皮肤基本一致。按中华医学会手外科学会上肢部分功能评定试用标准,患指指腹感觉为S4级;患指末梢两点辨别觉距离为6.0 ~9.0 mm,平均7.2 mm;功能为优7例、良4例,优良率100%。供瓣手指伸屈功能良好,切取侧指腹感觉为S4级。植皮区轻度凹陷,植皮边缘无瘢痕挛缩。结论指侧岛状皮瓣修复邻指掌侧皮肤软组织缺损,既可重建手指末梢血运、感觉,又可修复掌侧缺损创面,是一种较好的修复方法。
雌激素及其受体在风湿性疾病中的研究进展

目前关于雌激素及其受体与风湿性疾病关系的研究越来越多,不论是激素水平、蛋白水平,或者基因水平等方面的研究,都提示了雌激素及其受体与风湿性疾病的发生发展有着密切的联系,但是雌激素及其受体在风湿性疾病中的具体作用机制尚不清楚。进一步加强对雌激素及其受体的生物学功能及其参与风湿性疾病病理过程作用机制的研究,有望为风湿性疾病的预防、诊断提供新的思路,并为风湿性疾病的治疗提供新的靶点。
节律性夹带在康复医学中的应用及治疗进展

节奏性运动是人类日常生活最基本的一种运动方式,例如音乐、舞蹈、步行、进食等。近年来对节律性运动的研究发现,这种连续运动在行为学上有一个独特现象,即生物体以内在的节律同步于外在节奏的现象,称为节律性夹带(rhythmic entrainment,RE),或称节律性共鸣、拖带等。以节律性夹带为基础的康复训练利用个体运动同步到节律性视听线索的能力,进而提高患者运动、言语、认知的能力,越来越受到人们的重视。随着研究的不断深入,节律性夹带的理念和技术在临床康复实践中已经有所应用,本文就这一新的理念做一综述,为其相关领域研究提供理论参考。
虚拟现实训练联合心理干预对脑卒中后抑郁的影响

脑卒中后抑郁(post-stroke depression, PSD)是脑卒中后常见的并发症之一,严重影响患者功能康复及日常生活质量。目前,药物疗法是治疗脑卒中后抑郁的主要手段,长期使用会产生明显不良反应,存在增加心血管疾病的风险和加重抑郁的可能性。常规康复治疗手段容易使患者产生厌倦感,积极性和配合度不高。因此,寻求改善卒中患者抑郁情绪的有效康复措施显得尤为重要。虚拟现实(virtual reality, VR)技术是一门新兴的人机交互技术,广泛应用于多感官教学、心理治疗、康复训练等实践领域,它是利用计算机生成一种模拟真实事物的虚拟环境,并通过多种传感设备使用户“投入”到该环境中,综合了视、听、触觉感受,使患者产生一种身临其境的感觉,具有沉浸性、交互性、构想性等特点。
Emergency Plan for Water Supply in Consecutive Droughts and Sustainable Water Resources Management in Beijing

Beijing, the capital city of China, has suffered from acute water shortage, with only 300 m3/a of water resources available per capita. In addition, Beijing has experienced a prolonged period of consecutive droughts from 1999 to 2010. Water crisis has constrained the socio-economic development of Beijing. Meanwhile, the national "South-to-North Water Transfer" (STNWT) project, which is expected to provide some relief to the water crisis in Beijing, is still under development. In order to ensure the security of water supply in Beijing before the completion of the STNWT project, several measures have been implemented to cope with droughts, including pumping groundwater from emergency well fields, water saving, recycling of water, rain and flood water harvesting, and the diversion of water from neighboring rivers and groundwater basins. Groundwater from four emergency well fields contributes the most to the public and domestic water supplies in Beijing, supplying a total volume of 1.8×10~9 m~3. The water crisis is supposed to be mitigated by the completion of the STNWT project. After the completion of this project, more sustainable management of water resources will be implemented, including the use of aquifers as groundwater reservoirs and conjunctive use of surface water and groundwater resources.
SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation

Regulation of transcription is fundamental to the control of cellular gene expression and function. Although recent studies have revealed a role for the oncoprotein MYC in amplifying global transcription, little is known as to how the global transcription is suppressed. Here we report that SUMO and MYC mediate opposite effects upon global transcription by controlling the level of CDK9 sumoylation. On one hand, SUMO suppresses global transcription via sumoylation of CDK9, the catalytic subunit of P-TEFb kinase essential for productive transcriptional elongation. On the other hand, MYC amplifies global transcription by antagonizing CDK9 sumoylation. Sumoylation of CDK9 blocks its interaction with Cyclin T1 and thus the formation of active P-TEFb complex. Transcription profiling analyses reveal that SUMO represses global transcription, particularly of moderately to highly expressed genes and by generating a sumoylation-resistant CDK9 mutant, we confirm that sumoylation of CDK9 inhibits global transcription. Together, our data reveal that SUMO and MYC oppositely control global gene expression by regulating the dynamic sumoylation and desumoylation of CDK9.
Dimethyl fumarate: targeting glycolysis to treat MS

Dimethyl fumarate (DMF) is an immunomodulatory drug used in patients with multiple sclerosis (MS) and psoriasis. Kornberg and colleagues shed new light on DMF activity, demonstrating that it blocks immune cell activation through inhibition of glycolysis via post-transcriptional modification of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which confirms the pro-inflammatory role of glycolysis and points to potential new targets to treat inflammatory diseases such as MS.
伴有上运动神经元体征的腓骨肌萎缩症的致病基因研究进展

腓骨肌萎缩症(Charcot-Marie-Tooth disease, CMT)是一组具有高度临床和遗传异质性的单基因遗传性运动感觉神经病(hereditary motor and sensory neuropathy, HMSN)。 此组疾病多于儿童和青少年时期起病,临床上以缓慢进展的对称性肢体远端肌肉无力与萎缩、轻至中度末梢型感觉障碍、腱反射减弱或消失和高弓足为典型表现。依据神经电生理及神经病理特点,CMT主要分为脱髓鞘型(CMT1)和轴索型(CMT2)两型。近年来,CMT中间型(DI-CMT)正逐渐被认识。
我国晚期胃癌临床特点及诊治策略

我国新发胃癌患者约占全世界的40%,据2015年统计数据我国有87.3万现患胃癌患者。我国胃癌患者与其他胃癌高发国家相比,突出特点是晚期胃癌占比较高,约30% ~40%属无法根治的晚期胃癌即临床Ⅳ期,这部分患者疗效极差。随着肿瘤规范化治疗的推广应用,早期及中期胃癌受到极大关注,疗效获得显著提升。而晚期胃癌作为胃癌治疗领域的“最后一块高地”,始终缺乏有效的治疗手段。本文归纳总结我国晚期胃癌的临床特点,提出针对晚期胃癌的诊治策略,期冀能够对我国晚期胃癌的临床诊治有所助益。
Treg/Th17细胞比例失衡在弥漫大B细胞淋巴瘤预后中的意义

弥漫大B细胞淋巴瘤(DLBCL)在我国约占所有非霍奇金淋巴瘤(NHL)的35%~50%。DLBCL的发生、发展常伴有机体免疫功能的抑制和损害。Treg细胞具有抗炎性反应和维持自身免疫耐受的功能;分泌较高水平细胞因子IL-17的Th17细胞具有强大的促炎效应。两者从发育分化过程来看是相互排斥的,在调节自身免疫或细菌感染所致炎症的免疫应答过程中发挥着互相拮抗的功能。已有研究证实Treg/Th17细胞比例失衡在某些血液肿瘤的发生和进展中具有重要作用,但该平衡状态与DLBCL患者预后是否存在相关性尚未见文献报道。本研究旨在探讨Treg/Th17细胞比例失衡在患者预后评价中的意义。
Influence of particle shape on the erodibility of non-cohesive soil: Insights from coupled CFD-DEM simulations

Soil erosion is a critical process that is being studied in soil science, hydraulic engineering, and geotechnical engineering. Among many societal and environmental impacts, soil erosion is a major cause for the failures of bridges. The erodibility of soil is determined by its physical and geochemical properties and is also affected by surrounding biological activities. In most of the current models for soil erosion, erodibility of non-cohesive soil is characterized by its median grain size (D_(50)), density, and porosity. The contribution to erodibility of the irregular shape of soil grains, which plays an important role in the mechanical and hydraulic properties of coarse-grained soils, is generally ignored. In this paper, a coupled computational fluid dynamics and discrete element method model is developed to analyze the influence of the shape of sand grain on soil erodibility. A numerical model for the drag force on spherical and non-spherical particles is verified by using the results from physical free settling experiments. Erosion of sand grains of different shapes is simulated in a virtual erosion function apparatus, a laboratory device used to measure soil erodibility. The simulation results indicate that the grain shape has major effects on erodibility. Spherical particles do not show a critical velocity because of their low rolling resistance, but a critical velocity does exist for angular particles owing to grain interlocking. The erosion rate is proportional to the flow velocity for both spherical and non-spherical particles. The simulation result for angular particle erosion is fairly consistent with the experimental observations, implying that grain shape is an important factor affecting the erodibility of non-cohesive soils.
The symbol of spread of modern Western botany into China: Chih-wu hsueh, an unconventional translation in the late Qing dynasty

Traditional botany in ancient China mainly included agriculture and medicine. In contrast, botany in the broader modern sense was introduced from the West and established in China gradually. In 1858, the London Missionary Society Press (Mo Hai Shu Kuan) in Shanghai published a translation titled Chih-wu hsueh (Botany) (Fig. 1),which was compiled and translated by Alexander Williamson and Joseph Edkins, two British missionaries (Fig. 2),and Shanlan Li (Fig. 3),an exceptional scholar in the late Qing dynasty. As the first translated work on modern Western botany, this translation signified the spread of modern Western botany into China. Therefore, it served as the enlightenment in the history of modern Chinese botany and was regarded as a historic event in the history of the eastward spread of Western learning. The publication of Chih-wu hsueh was an example of the successful scientific and cultural interaction between Western and Chinese ideas because it has been acknowledged as the fusion of traditional Chinese botany and modern Western botany.
右上臂神经脂肪瘤病合并巨指症一例

讨论神经脂肪瘤病是一种罕见的易发于四肢周围神经的良性脂肪瘤样病变,以正中神经最易累及,亦可累及尺神经、桡神经、臂丛神经、下肢神经及坐骨神经,受累神经增粗变形,神经束被纤维脂肪组织包绕并相互分离,围绕在神经束周围的神经束细胞及周围纤维组织呈同心圆样或假洋葱皮样排列。神经脂肪瘤病大多因累及正中神经引起前臂、腕掌部肿块为首要临床表现就诊,部分患者合并巨指症。病因尚不明确。术前MRI的特征性表现为病变部位有代表着神经被周围脂肪组织均匀包绕的葡行样结构低信号影,合并巨指症时手部X线检查也比较重要。治疗的主要方法为手术,手术时注意保护神经,合并巨指症时依据体积大小分型治疗为佳,重型可采用截指术。
Elimination of ceftiofur hydrochloride residue in postpartum cows' milk after intramammary infusing at dry-off

The purpose of this study was to investigate the residue elimination of ceftiofur hydrochloride in milk of postpartum cows after intramammary infusing at dry-off. An ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/ MS) method was developed and validated to detect ceftiofur hydrochloride residue in milk. Through preprocessing, ceftiofur hydrochloride was derivatized into a more stable compound dedfuroyl ceftiofur acetamide(DCA) for further analysis. The linear range of DCA was 0.1 to 50 μg kg~(–1). Average recoveries of DCA were 82.52–105.86%. The intra-day and interday coefficients of variation(CV) were 2.95–9.82 and 6.41–7.43%, respectively. The limit of detection(LOD) and limit of quantitation(LOQ) scores were 0.05 and 0.1 μg kg~(–1), respectively. These parameters showed this method was reliable and valid. Twelve cows were administrated 10 mL ceftiofur hydrochloride by intramammary infusion(corresponding to 500 mg ceftiofur) to each udder after the last milking before the dry-off period. Milk was collected from each udder of cow at 12, 24, 36, 48, 60 and 72 h after calving and was mixed for each time point and each cow, then subjected to UPLC-MS/MS analysis. The results showed, the DCA concentrations in all milk samples were less than LOQ and the maximum residue limit(MRL) 100 μg kg–1, which suggested the withdrawal time of ceftiofur hydrochloride intramammary infusion used for preventing and curing mastitis in dry cows was 0 day. The study provided guidance for the clinical applications of ceftiofur hydrochloride intramammary infusion(dry cow).
On the selection of solutions for mutation in differential evolution

Differential evolution (DE) is a kind of evolutionary algorithms, which is suitable for solving complex optimization problems. Mutation is a crucial step in DE that generates new solutions from old ones. It was argued and has been commonly adopted in DE that the solutions selected for mutation should have mutually different indices. This restrained condition, however, has not been verified either theoretically or empirically yet. In this paper, we empirically investigate the selection of solutions for mutation in DE. From the observation of the extensive experiments, we suggest that the restrained condition could be relaxed for some classical DE versions as well as some advanced DE variants. Moreover, relaxing the restrained condition may also be useful in designing better future DE algorithms.
Online clustering of streaming trajectories

With the increasing availability of modern mobile devices and location acquisition technologies, massive trajectory data of moving objects are collected continuously in a streaming manner. Clustering streaming trajectories facilitates finding the representative paths or common moving trends shared by different objects in real time. Although data stream clustering has been studied extensively in the past decade, little effort has been devoted to dealing with streaming trajectories. The main challenge lies in the strict space and time complexities of processing the continuously arriving trajectory data, combined with the difficulty of concept drift. To address this issue, we present two novel synopsis structures to extract the clustering characteristics of trajectories, and develop an incremental algorithm for the online clustering of streaming trajectories (called OCluST). It contains a micro-clustering component to cluster and summarize the most recent sets of trajectory line segments at each time instant, and a macro-clustering component to build large macro-clusters based on micro-clusters over a specified time horizon. Finally, we conduct extensive experiments on four real data sets to evaluate the effectiveness and efficiency of OCluST, and compare it with other congeneric algorithms. Experimental results show that OCluST can achieve superior performance in clustering streaming trajectories.
Smart manufacturing systems for Industry 4.0: Conceptual framework, scenarios, and future perspectives

Information and communication technology is undergoing rapid development, and many disruptive technologies, such as cloud computing, Internet of Things, big data, and artificial intelligence, have emerged. These technologies are permeating the manufacturing industry and enable the fusion of physical and virtual worlds through cyber-physical systems (CPS), which mark the advent of the fourth stage of industrial production (i.e., Industry 4.0). The widespread application of CPS in manufacturing environments renders manufacturing systems increasingly smart. To advance research on the implementation of Industry 4.0, this study examines smart manufacturing systems for Industry 4.0. First, a conceptual framework of smart manufacturing systems for Industry 4.0 is presented. Second, demonstrative scenarios that pertain to smart design, smart machining, smart control, smart monitoring, and smart scheduling, are presented. Key technologies and their possible applications to Industry 4.0 smart manufacturing systems are reviewed based on these demonstrative scenarios. Finally, challenges and future perspectives are identified and discussed.