mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
前言

心律失常是临床上常见的心血管疾病,心律失常起源于心脏电脉冲的反常性发放和电活动的转导异常。心律失常减损心脏的机械舒缩功能,影响心脏的泵血,严重时可导致休克、心力衰竭或者心源性猝死。心律失常影响了患者的生活质量,并对社会造成了严重的经济负担。心律失常的诊治已取得了长足进展,但令人遗憾的是,心律失常的发生机制、预警预报和干预仍然面临重大挑战。迄今为止,心房颤动、致命性心律失常等心律失常的发生机制依然莫衷一是。医学界严重缺乏心律失常的预警预报手段。心律失常的主要治疗策略包括药物、介入和手术治疗。然而,目前用于治疗心律失常的药物本身却存在触发心律失常的危险,并可以增加心律失常的死亡率;心律失常的介入和手术治疗也存在着损伤较大、成功率较低和高复发性的缺陷。本期推出心律失常专题,旨在介绍心律失常研究领域的相关前沿进展。
基于军车驾驶员的驾驶愤怒与驾驶愤怒表达问卷修订及应用研究

交通事故已成为威胁人类生命安全的第一公害。人为因素占交通事故成因的84% ~90%,其中驾驶愤怒与驾驶攻击是诱发道路交通事故的重要原因。驾驶愤怒是指由于驾驶过程中的压力与挫折情境产生的愤怒情绪倾向,驾驶攻击指因驾驶愤怒导致驾驶过程中的攻击行为。国外从60年代起开始驾驶愤怒与驾驶攻击研究,已研究出较为成熟的测试工具。Deffenbacher等研制的驾驶愤怒问卷(driving anger scale,DAS)和驾驶愤怒表达量表(driving anger expression inventory, DAX)是研究驾驶愤怒与驾驶攻击最常用的两种问卷,在发达国家已得到较为广泛的修订及应用,但我国鲜有报道。军车驾驶员由于担负着繁重的运输任务和各种抗洪抢险、抗震救灾等非战争军事应激任务,驾驶任务的高强度、高风险以及驾驶环境的恶劣及艰险使其长期处于高度的紧张状态,在面临愤怒情景时更容易触发驾驶愤怒及驾驶攻击的产生,并引发交通安全隐患。目前尚未见关于我国军车驾驶员的驾驶愤怒与驾驶攻击的研究。笔者基于我国军车驾驶员,对DAS、DAX进行修订,并分析军车驾驶员驾驶愤怒及驾驶攻击的现状,为进一步理解和干预我国军车驾驶员驾驶愤怒和驾驶攻击奠定理论基础。
木犀草素-7-O-芸香糖苷的半合成

目的设计橙皮苷一步法半合成木犀草素-7-0-芸香糖苷。方法橙皮苷与三氯化铝络合后溶于含吡啶的甲醇溶液中,经过碘脱氢,生成地奥明与铝的络合物,密闭蒸馏除去吡啶和甲醇,进行脱甲基反应,得到木犀草素-7-O-芸香糖苷。结果HPLC测定木犀草素含有量为38.2 g/kg、木犀草苷为75.0 g/kg、地奥司明为80.4 g/kg,扣除上述杂质后,木犀草素7-O-芸香糖苷含有量为806.4g/kg,收率为65.7%。结论该路线工艺简单,试剂用量少,反应条件温和,收率良好,具有一定的工业化生产潜力。
镁海水电池及镁阳极材料的研究进展

镁电极材料具有成本低、无毒、无污染、放电电压平稳、比能量及比功率高、资源丰富、可再生等诸多优点,但用作海水电池阳极仍存在自腐蚀速率大、阳极利用率低等问题。介绍了镁海水电池的种类,综述了近年来海水电池中镁阳极材料的研究进展,并展望了今后提高镁阳极材料性能的发展方向。
不同辅料对三黄粉吸湿性的影响

目的考察不同辅料对三黄粉吸湿性的影响,筛选防潮效果适宜的辅料。方法测定三黄粉添加不同辅料后的吸湿百分率,绘制吸湿曲线,采用Excel和Origin 7.5软件拟合曲线,建立数学模型,并分析直观表征吸湿能力的特性参数,即吸湿加速度和平衡吸湿率。结果添加30%乳糖可改善三黄粉吸湿性,其临界相对湿度为78%。结论本实验可为选择三黄粉的防潮辅料提供参考依据。
氢气对肺部疾病保护作用的研究进展

氢是自然界中最简单和丰富的化学元素,占所有元素质量的75%。氢气(H_2)是一种无色无味气体,以往因其相对的生理惰性,常被用于潜水医学领域。随着2007年Ohsawa等首次报道氢气具有选择性抗氧化功能,其生物学效应已受到广泛关注。同时,氢气的抗氧化、抗凋亡、抗炎以及抗过敏特性,使其在疾病的预防和治疗等领域也具有广阔的应用前景。
样品采集篇(6)

样品釆集篇共有6节,第一节矿产地质样品采集工作的任务与意义(上、下)、第二节矿产预查阶段的采样工作(上、下)和第三节矿产普查阶段的采样工作(上),已在本刊前5期刊登,本期刊登第三节矿产普查阶段的采样工作(下)。
澳大利亚布罗肯希尔(Broken Hill)铅锌银矿床

从全球范围来看,铅锌矿床的最主要类型是SEDEX型和MVT型。而SEDEX型铅锌矿床以单个矿床储量巨大、品位高为特征,位于澳大利亚新南威尔士州西北部的布罗肯希尔(Broken Hill)铅锌银矿床就是一个典型实例。它是世界上最大的铅锌银矿床之一,矿石储量2.8亿吨,矿石品位:w(Pb)为10%;w(Zn)为8.5%; w(Ag)为148 g/t(Walters,1996),自1883年发现以来,已开采矿石约2亿吨。
Drug screening:zebrafish as a tool for studying epileptic-related chemical compounds

Here we report findings of zebrafish behavior and development during drug screening. In developing zebrafish embryos, epileptic seizure-like locomotor defects can be induced by brief treatment with GABA_A receptor antagonist pentylenetetrazole (PTZ). Using a trajectory video tracking system, we recorded zebrafish movements and constituted convulsant-like locomotor behaviors after treatment with PTZ. This led to the development of the first 3-D trace map of zebrafish locomotor behaviors in response to drug treatment. In addition, we examined the dose-dependent rescues of the locomotor defects in PTZ-treated animals after treatment with anti-epileptic drugs, such as valproate (VPA). We also examined the effects of drug treatment on animal development, such as hatching and mortality. Together, this research sheds new light for the development of new strategies for high-throughput drug screens using zebrafish models.
癫痫患者院前发作时发生意外伤的相关因素及对策

目的了解癫痫患者院前发作时发生意外伤情况及其相关因素,为制定相应护理干预措施提供参考。方法便利抽样选择厦门市四所三级甲等医院意外伤人院癫痫患者共141例,采用自制调查表对其进行问卷调查分析。结果癫痫患者院前发作时发生意外伤与患者的年龄、文化程度、经济情况、家庭功能、社会功能呈负相关(均P<0.05);与病程、性别、居住地没有相关性(均P>0.05)。多元回归显示,癫痫患者院前发作时发生意外伤情况与其文化程度、社会支持、病程呈显著相关(均P<0.05)。结论癫痫患者院前发作时发生意外伤的情况严峻,应根据患者不同的社会背景及自身条件予以个体化护理及安全教育,鼓励其建立自我护理意识是降低意外伤发生率的重要措施。
Neuronostatin的发现及其生理学功能的研究进展

Neuronostatin(简称NST)是最近刚发现的由生长抑素基因编码的另一种神经肽,它与生长抑素是由同一前体蛋白经过不同的加工过程而产生的,然而二者的结构与生理学功能是不同的。不同于生长抑素的环状结构,NST是由13个氨基酸组成的线性肽,并且其C端是酰胺化的。同生长抑素类似,NST分布广泛,参与了神经元功能调控、血压调控、摄食及饮水等。本文简要概括NST及其受体的研究进展,旨在加深对NST生理功能的认识和了解,为进一步研究作一铺垫。
腹腔镜手术在妇科恶性肿瘤治疗中应用的现状

随着科学技术的飞速发展,各种科学技术渗入医学,推动了临床诊断和治疗的进步,内镜技术、新能源、新材料不断被开发和应用。妇科肿瘤的诊治在这样的大环境下有了长足的进展。同样,在这样一个观念改变的时代,微创的要求、患者的精神及美学需求更突显了重要地位。妇科肿瘤医师在不断地追求着以最小的手术创伤达到更佳的治疗效果,经过不断的实践和努力,腹腔镜手术得已在妇科恶性肿瘤治疗中应用,并迅猛发展、日臻完善。
Improved anti-tumor activity and safety profile of a paclitaxel-loaded glycyrrhetinic acid-graft-hyaluronic acid conjugate as a synergistically targeted drug delivery system

The present study was designed to develop and evaluate glycyrrhetinic acid-graft-hyaluronic acid (HGA) conjugate for intravenous paclitaxel (PTX) delivery. Lyophilized PTX-loaded self-assembled HGA nanoparticles (PTX/HGAs) were prepared and characterized by dynamic light scattering measurements. Hemolysis test, intravenous irritation assessment, and in vitro and in vivo pharmacodynamic studies were carried out. B16F10 and HepG2 cells were used in the cell apoptosis analysis. The mouse MDA-MB-231 xenograft model was used for the evaluation of in vivo anticancer activity of the drugs, by the analysis of tumor growth and side effects on other tissues. PTX/HGAs showed high stability and good biocompability. Compared with PTX plus GA plus HA solution, PTX/HGAs displayed obvious superiority in inducing the apoptosis of the cancer cells. Following systemic administration, PTX/HGAs efficiently suppressed tumor growth, with mean tumor inhibition ratio (TIR) being 65.08%, which was significantly higher than that of PTX plus GA plus HA treatment. In conclusion, PTX/HGAs demonstrated inhibitory effects tumor growth without unwanted side effects, suggesting that HGA conjugates hold a great potential as a delivery carrier for cancer chemotherapeutics to improve therapeutic efficacy and minimize adverse effects.
Immunomodulatory activity of macromolecular polysaccharide isolated from Grifola frondosa

The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa (GFP) by using mouse peritoneal macrophage and cytoxan (CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A (one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing ConA or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the mRNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4~+ and CD8~+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.
Transgenic Arabidopsis thaliana expressing a wheat oxalate oxidase exhibits hydrogen peroxide related defense response

Oxalic acid (OA) is considered as an important pathogenetic factor of some destructive diseases caused by some fungal pathogens such as Sclerotinia sclerotiorum. Oxalate degradation is important for plant health, and plants that contain oxalate oxidase (OXO) enzymes could breakdown oxalate into CO_2 and H_2O_2, which subsequently evokes defense responses. However, some species, such as Arabidopsis thaliana, have no oxalate oxidase activity identified to date. The present study aims to develop transgenic Arabidopsis expressing a wheat oxalate oxidase, to test for the response to OA exposure and fungal infection by S. sclerotiorum. The results showed that the transgenic Arabidopsis lines that expressed the wheat OXO exhibited enhanced resistance to OA exposure and S. sclerotiorum infection in the tolerance assays. In the same manner, it could convert OA to CO_2 and H_2O_2 to a higher extent than the wild-type. Intensive osmotic adjustments were also detected in the transgenic Arabidopsis lines. The higher level of produced H_2O_2 subsequently induced an elevated activity of antioxidant enzymes including superoxide dismutase (SOD) and peroxidase (POD) in the transgenic Arabidopsis plants. The present study indicated that the expression of a gene encoding wheat OXO could induce intensive osmotic adjustments and hydrogen peroxide related defense response, and subsequently increased tolerance to S. sclerotiorum in transgenic A. thaliana.
Effects of long-term full straw return on yield and potassium response in wheat-maize rotation

The effect of long-term straw return on crop yield, soil potassium (K) content, soil organic matter, and crop response to K from both straw and chemical K fertilizer (K_2SO_4) were investigated in a fixed site field experiment for winter wheat-summer maize rotation in 6 years for 12 seasons. The field experiment was located in northern part of North China Plain with a sandy soil in relatively low yield potential. Two factors, straw return and chemical K fertilizer, were studied with two levels in each factor. Field split design was employed, with two straw treatments, full straw return of previous crop (St) and no straw return, in main plots, and two chemical K fertilizer treatments, 0 and 60 kg K_2O ha~(-1), as sub-plots. The results showed that straw return significantly increased yields of winter wheat and summer maize by 16.5 and 13.2% in average, respectively, and the positive effect of straw return to crop yield showed more effective in lower yield season. Straw return significantly increased K absorption by the crops, with significant increase in straw part. In treatment with straw return, the K content in crop straw increased by 15.9 and 21.8% in wheat and maize, respectively, compared with no straw return treatment. But, straw return had little effect on K content in grain of the crops. Straw return had significant influences on total K uptake by wheat and maize plants, with an increase of 32.7 and 30.9%, respectively. There was a significant correlation between crop yield and K uptake by the plant. To produce 100 kg grain, the wheat and maize plants absorbed 3.26 and 2.24 kg K_2O, respectively. The contents of soil available K and soil organic matter were significantly affected by the straw return with an increase of 6.07 and 23.0%, respectively, compared to no straw return treatment. K_2SO_4 application in rate of 60 kg K_2O ha~(-1) showed no significant effect on wheat and maize yield, K content in crop straw, total K uptake by the crops, soil available K content, and soil organic matter. The apparent K utilization rate (percentage of applied K absorbed by the crop in the season) showed difference for wheat and maize with different K sources. In wheat season, the K utilization rate from K_2SO_4 was higher than that from straw, while in maize season, the K utilization rate from straw was higher than that from chemical fertilizer. In the whole wheat-maize rotation system, the K absorption efficiency by the two crops from straw was higher than that from K_2SO_4.
Integrated application of February Orchid (Orychophragmus violaceus) as green manure with chemical fertilizer for improving grain yield and reducing nitrogen losses in spring maize system in northern China

The development of more efficient management systems is crucial to achieving high grain yields with high nitrogen use efficiency (NUE). February Orchid-spring maize rotation system is a newly established planting system with the benefits of ground cover and potential wind erosion in northern China. A field experiment was conducted to evaluate the effects of integrated application of February Orchid as green manure with reduction of chemical fertilizers (INTEGRATED) on spring maize yield, N uptake, ammonium volatilization, and soil residual mineral N in northern China. Compared to farmers’ traditional fertilization (CON), integrated application of February Orchid as green manure with 30% reduction of nitrogen fertilizers (INTEGRATED) increased maize grain yield and biomass by 9.9 and 10.2%, respectively. The 0-100 cm soil residual N_(min) at harvest was decreased by 58.5% and thus nitrogen use efficiency was increased significantly by 26.7%. The nitrogen balance calculation further demonstrated that the INTEGRATED approach performed better than CON with lower apparent nitrogen loss (decreased by 48.9%) which evidenced by the ammonium volatilization of top-dressing fertilizer was decreased by 31.1%, the N_(min) movement to the deeper soil layers was reduced, and the apparent nitrogen leaching loss nearly equal to 0 under the INTEGRATED treatment. Therefore, in northern China, integrated application of green manure and chemical fertilizers is an efficient management approach for improving maize yields and NUE simultaneously.
Herbivore defense responses and associated herbivore defense mechanism as revealed by comparing a resistant wild soybean with a susceptible cultivar

Plants have evolved sophisticated defensemechanisms against herbivores to help them adapt to the environment. Understanding the defense mechanisms in plants can help us control insects in a more effective manner. In this study, we found that compared with Tianlong 2 (a cultivated soybeanwith insect susceptibility), ED059 (a wild soybean linewith insect resistance) contains sharper pubescence tips, as well as lower transcript levels of wound-induced protein kinase (WIPK) and salicylic acid-induced protein kinase (SIPK), which are important mitogen-activated protein kinases involved in early defense response to herbivores. The observed lower transcript levels of WIPK and SIPK induced higher levels of jasmonic acid (JA), JA biosynthesis enzymes (AOC3) and some secondarymetabolites in ED059. Functional analysis of the KTI1 gene via Agrobacterium-mediated transformation inArabidopsis thaliana indicated that it plays an important role in herbivore defense in ED059. We further investigated the molecular response of third-instar Helicoverpa armigera (Hubner) larvae to Tianlong 2 and ED059.We found apoptotic cells only in themidguts of larvae that fed on ED059. Comparedwith larvae reared on the susceptible cultivar Tianlong 2, transcript levels of catalase (CAT) and glutathione S-transferase (GST) were up-regulated, whereas those of CAR, CHSB, and TRY were down-regulated in larvae that fed on the highly resistant variety ED059. We propose that these differences underlie the different herbivore defense responses of ED059 and Tianlong 2.
Long-term organic and inorganic fertilizations enhanced basic soil productivity in a fluvo-aquic soil

The improvement of soil productivity depends on a rational input of water and nutrients, optimal field management, and the increase of basic soil productivity (BSP). In this study, BSP is defined as the productive capacity of a farmland soil with its own physical and chemical properties for a specific crop season under local field management. Based on 19-yr data of the long-term agronomic experiments (1989-2008) on a fluvo-aquic soil in Zhengzhou, Henan Province, China, the decision support system for agrotechnology transfer (DSSAT ver. 4.0) crop growth model was used to simulate yields by BSP of winter wheat (Triticum aestivium L.) and summer maize (Zea mays L.) to examine the relationship between BSP and soil organic carbon (SOC) under long-term fertilization. Five treatments were included: (1) no fertilization (control), (2) nitrogen, phosphorus and potassium fertilizers (NPK), (3) NPK plus manure (NPKM), (4) 1.5 times of NPKM (1.5NPKM), and (5) NPK plus straw (NPKS). After 19 yr of treatments, the SOC stock increased 16.7, 44.2, 69.9, and 25.2% under the NPK, NPKM, 1.5NPKM, and NPKS, respectively, compared to the initial value. Among various nutrient factors affecting contribution percentage of BSP to winter wheat and summer maize, SOC was a major affecting factor for BSP in the fluvo-aquic soil. There were significant positive correlations between SOC stock and yields by BSP of winter wheat and summer maize (P<0.01), and yields by BSP of winter wheat and summer maize increased 154 and 132 kg ha~(-1) when SOC stock increased 1 t C ha~(-1). Thus, increased SOC accumulation is a crucial way for increasing BSP in fluvo-aquic soil. The manure or straw combined application with chemical fertilizers significantly enhanced BSP compared to the application of chemical fertilizers alone.
Review grain yield and nitrogen use efficiency in rice production regions in China

As one of the staple food crops, rice (Oryza sativa L.) is widely cultivated across China, which plays a critical role in guaranteeing national food security. Most previous studies on grain yield or/and nitrogen use efficiency (NUE) of rice in China often involved site-specific field experiments, or small regions with insufficient data, which limited the representation for the current rice production regions. In this study, a database covering a wide range of climate conditions, soil types and field managements across China, was developed to estimate rice grain yield and NUE in various rice production regions in China and to evaluate the relationships between N rates and grain yield, NUE. According to the database for rice, the values of grain yield, plant N accumulation, N harvest index (HI_N), indigenous N supply (INS), internal N efficiency (IE_N), reciprocal internal N efficiency (RIE_N), agronomic N use efficiency (AE_N), partial N factor productivity (PEP_N), physiological N efficiency (PE_N), and recover efficiency of applied N (RE_N) averaged 7.69 t ha~(-1), 152 kg ha~(-1), 0.64 kg kg~(-1), 94.1 kg kg~(-1), 53.9 kg kg~(-1), 1.98 kg kg~(-1), 12.6 kg kg~(-1), 48.6 kg kg~(-1), 33.8 kg kg~(-1), and 39.3%, respectively. However, the corresponding values all varied tremendously with large variation. Rice planting regions and N rates had significant influence on grain yield, N uptake and NUE values. Considering all observations, N rates of 200 to 250 kg ha~(-1) commonly achieved higher rice grain yield compared to less than 200 kg N ha~(-1) and more than 250 kg N ha~(-1) at most rice planting regions. At N rates of 200 to 250 kg ha~(-1), significant positive linear relationships were observed between rice grain yield and AE_N, PE_N, RE_N, IE_N, and PFP_N, and 46.49, 24.64, 7.94, 17.84, and 88.24% of the variation in AE_N, PE_N, RE_N, IE_N, and PFP_N could be explained by grain yield, respectively. In conclusion, in a reasonable range of N application, an increase in grain yield can be achieved accompanying by an acceptable NUE.
Determination of optimum nitrogen application rates in Zhejiang Province, China, based on rice yields and ecological security

Excessive nitrogen (N) fertilization in intensive agricultural areas such as the plain region of South China has resulted in low nitrogen use efficiency and serious environmental problems. To determine the optimum N application rate, grain yield, apparent nitrogen recovery efficiency (ANRE), apparent N loss, and ammonium (NH3) volatilization under different N application rates in the three years from 2012 to 2014 were studied. The results showed that the relationship between grain yields and N application rate in the three years were well fitted by quadratic equations. When N application rate reached 197 kg ha~(-1) in 2012, 199 kg ha~(-1) in 2013 and 196 kg ha~(-1) in 2014, the plateau of the grain yields appeared. With the increase of N application rate, the ANRE for rice decreased which could be expressed with sigmoidal equation; when N application rate was 305 kg ha~(-1) in 2012, 275 kg ha~(-1) in 2013 and 312 kg ha~(-1) in 2014, the curves of ANRE appeared turing points. Besides, the relationship between soil N_(residual) and N application rate was fitted by the quadratic equation and the maximums of soil N_(residual) were reached in the three years with the N application rate of 206, 244 and 170 kg ha~(-1), respectively. Statistical analysis indicated that NH_3 volatilization and apparent N loss in three years all increased with the increasing N application rate. When the amount of NH_3 volatilization increased to 11.6 kg N ha~(-1) in 2012, 40.5 kg N ha~(-1) in 2013 and 57.0 kg N ha~(-1) in 2014, the apparent N loss in the three years had obvious increase. To determine the optimum N application rate, the average N application on the plateau of the grain yield was considered as the lower limit while the average N application rate at the turning points of ANRE, the residual N in soil and apparent N loss was taken as the upper limit. According to the results in three years, the optimum N application rate for rice in Zhejiang was 197-255 kg ha~(-1).
Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China

On the basis of a long-term (30 years) field experiment that involved four rotation systems, rice-rice-winter fallow (RRF), rice-rice-ryegrass (RRG), rice-rice-rape (RRP), and rice-rice-milk vetch (RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16S rRNA gene. The Chao1 richness and non-parametric Shannon’s index increased in all soil samples that received green manure treatments. The communities’ structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.
Soil phosphorus dynamic, balance and critical P values in long-term fertilization experiment in Taihu Lake region, China

Phosphorus (P) is an important macronutrient for plant but can also cause potential environmental risk. In this paper, we studied the long-term fertilizer experiment (started 1980) to assess the soil P dynamic, balance, critical P value and the crop yield response in Taihu Lake region, China. To avoid the effect of nitrogen (N) and potassium (K), only the following treatments were chosen for subsequent discussion, including: C0 (control treatment without any fertilizer or organic manure), CNK treatment (mineral N and K only), CNPK (balanced fertilization with mineral N, P and K), MNK (integrated organic manure and mineral N and K), and MNPK (organic manure plus balanced fertilization). The results revealed that the response of wheat yield was more sensitive than rice, and no significant differences of crop yield had been detected among MNK, CNPK and MNPK until 2013. Dynamic and balance of soil total P (TP) and Olsen-P showed soil TP pool was enlarged significantly over consistent fertilization. However, the diminishing marginal utility of soil Olsen-P was also found, indicating that high-level P application in the present condition could not increase soil Olsen-P contents anymore. Linear-linear and Mitscherlich models were used to estimate the critical value of Olsen-P for crops. The average critical P value for rice and wheat was 3.40 and 4.08 mg kg~(-1), respectively. The smaller critical P value than in uplands indicated a stronger ability of P supply for crops in this paddy soil. We concluded that no more mineral P should be applied in rice-wheat system in Taihu Lake region if soil Olsen-P is higher than the critical P value. The agricultural technique and management referring to activate the plant-available P pool are also considerable, such as integrated use of low-P organic manure with mineral N and K.