mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Terrain matching localization for hybrid underwater vehicle in the Challenger Deep of the Mariana Trench

The maximum ocean depth so far reported is about 11 000 m,and is located in the Mariana Trench in the Western Pacific Ocean.The hybrid unmanned underwater vehicle,Haidou,is developed to perform scientific survey at the deepest parts of the Earth oceans.For vehicles working at the full-ocean depth,acoustic positioning is the most effective and popular method.The 11 000 m class acoustic positioning system is relatively massive and complex,and it requires specialized research vessels equipped with compatible acoustic instruments.As a compact testbed platform,it is impractical for Haidou to carry an LBL/USBL beacon with its large volume and weight.During the descent to about 11 000 m,horizontal drift could not be eliminated because of the hydrodynamics and uncertain ocean currents in the sea trials.The maximum depth recorded by Haidou is 10 905 m,and determining the precise location of the deepest point is challenging.With the bathymetric map produced by a multibeam sonar,the terrain contour matching (TERCOM) method is adopted for terrain matching localization.TERCOM is stable in providing an accurate position because of its insensitivity to the initial position errors.The final matching results show the best estimate of location in the reference terrain map.
Caryophyllene-type sesquiterpenoids and α-furanones from the plant endophytic fungus Pestalotiopsis theae

Two new caryophyllene-type sesquiterpenoids,pestathenols A (1) and B (2) and one new α-furanone,pestatheranone A (6),along with five known compounds (3-5,7 and 8) have been isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae.Their structures were unambiguously established by extensive spectroscopic analyses.The absolute configuration of the 5,6-diol moiety in 1 was assigned using Snatzke’s method.Compounds 1 and 2 showed weak cytotoxicity against HeLa cell line.
Improved soil characteristics in the deeper plough layer can increase grain yield of winter wheat

In the North China Plain (NCP),soil deterioration threatens winter wheat (Triticum aestivum L.) production.Although rotary tillage or plowing tillage are two methods commonly used in this region,research characterizing the effects of mixed tillage on soil characteristics and wheat yield has been limited.A fixed-site field trial was carried out during 2011-2016 to examine the impacts of three tillage practices (5-year rotary tillage with maize straw removal (RT);5-year rotary tillage with maize straw return (RS);and annual RS and with a deep plowing interval of 2 years (RS/DS)) on soil characteristics and root distribution in the plough layer.Straw return significantly decreased soil bulk density,increased soil organic carbon (SOC) storage and SOC content,macro-aggregate proportion (R_(0.25)) and its stability in the plough layer.The RS/DS treatment significantly increased the SOC content,total nitrogen (TN),and root length density (RLD) in the 10-40 cm layer,and enhanced the proportion of RLD in the 20-30 and 30-40 cm layers.In the 20-30 and 30-40 cm layers,an increase in SOC and TN could lead to higher grain production than commensurate increases in the surface layer,resulting in a sustainable increase in grain yield from the RS/DS treatment.Thus,the RS/DS treatment could lead to high productivity of winter wheat by improving soil characteristics and root distribution at the deeper plough layer in the NCP.
Development and identification of glyphosate-tolerant transgenic soybean via direct selection with glyphosate

Glyphosate-tolerant soybean is the most widely planted genetically modified crop worldwide.However,soybean remains recalcitrant to routine transformation because of the low infection efficiency of Agrobacterium to soybean and lack of useful selectable markers.In this study,several Agrobacterium strains and cell densities were compared by transient expression of the GUS gene.The results showed that Agrobacterium strain Ag10 at cell densities of OD_(600) of 0.6-0.9 yielded the highest infection efficiency in Agrobacterium-mediated soybean cotyledonary node transformation system.Meanwhile,a simple and rapid method was developed for identification of glyphosate tolerance in putative T_0 transgenic plants,consisting of spotting plantlets with 1 μL Roundup®.The whole cycle of genetic transformation could be shortened to about 3 mon by highly efficient selection with glyphosate during the transformation process and application of the spot assay in putative T0 transgenic plantlets.The transformation frequency ranged from 2.9 to 5.6%.This study provides an improved protocol for development and identification of glyphosate-tolerant transgenic soybeans.
Tissue-specific expression and correlation with promoter DNA methylation of the LBP gene in pigs

Lipopolysaccharide binding protein (LBP) is a key factor in the recognition of lipopolysaccharide (LPS) and the initiation of immune response, thus regulating the body's resistance to pathogenic infection. To investigate the tissue-specific expression characteristics of the LBP gene and its transcriptional regulation in pigs, we detected LBP expression in different tissues of 35-day-old Meishan weaned piglets, determined LBP core promoter region using bioinformatics prediction combined with dual luciferase activity assay, and finally detected methylation levels by pyrosequencing. The results showed that LBP expression in the liver tissue was significantly higher (P<0.01) than that in other tissues, followed by the intestinal tissues. The core promoter region of LBP was located at –500–(–206) bp (chr.17: g.46837534–g.46837828), containing three CpG sites (CpG1, CpG2 and CpG3). Of the three CpG sites, CpG2 and CpG3 were variously methylated (P<0.01) in different tissues. Moreover, LBP mRNA levels were negatively correlated (P<0.01) with methylation levels of the CpG2 and CpG3 sites in the YY1 transcription factor binding sequence. It is speculated that the methylation of CpG2 and CpG3 sites might inhibit YY1 binding to the promoter sequences, thereby regulating the tissue-specific expression of LBP. This study demonstrated the distinct patterns of LBP expression and promoter methylation in the tissues of Meishan pigs and indicated the potential roles of DNA methylation in regulating LBP expression, which may contribute to further investigations on pig LBP gene expression and function.
The CRISPR/Cas9 induces large genomic fragment deletions of MSTN and phenotypic changes in sheep

The CRISPR/Cas9 system has been extensively used to engineer genetic loci for the generation of knockouts, insertions, and point mutations in animal models. However, many mutations that have been reported in animals are small insertions or deletions. This study used the CRISPR/Cas9 system to induce large DNA fragment deletions in MSTN via three guide RNAs in sheep. This successfully achieved the precise gene editing of the ovine MSTN gene by injecting both Cas9 mRNA and sgRNAs into embryos at the one-cell stage. Of 10 edited animals, 3 animals (30%) exhibited large genomic fragment deletions (~5 kb). Furthermore, the body weights of these 3 animals were significantly different (P_0<0.0001, P_(15)=0.001, P_(30)=0.005, P_(60)=0.027) between lambs with large deletions and wildtype lambs. In addition, the edited lambs were also significantly different (P_0<0.0001, P_(15)<0.0001, P_(30)=0.002, P_(60)=0.011) compared with wildtype. These results suggest that the generated MSTN knockout sheep is a reliable and effective animal model for further study. Furthermore, this method is time-and labor-saving, and efficient for the creation of animal models for agriculture, biology, and medicine.
Detection of seven phytohormones in peanut tissues by ultra-highperformance liquid chromatography-triple quadrupole tandem mass spectrometry

Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural production. In this study, an ultra-high-performance liquid chromatography-mass spectrometry/ mass spectrometry method was established for separation and quantification of trans-zeatin, trans-zeatin riboside, gibberellin A3, indol-3-acetic acid, salicylic acid, abscisic acid, and jasmonic acid (JA) without any label. The separation was performed on an Agilent Explus Plus C_(18) column by using methanol and water as mobile phases with gradient elution. The target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin, transzeatin riboside, indole-3-acetic acid, and via negative electrospray ionization for gibberellin3, salicylic acid, abscisic acid, and JA. The limits of detection ranged from 0.0127 ng L~(–1) for gibberellin A_3 (GA_3) to 33.26 ng L~(–1) for JA and were lower than the currently reported values in literature. The proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and pods. The recoveries of the spiked phytohormones ranged from 80.20 to 102.56%. The contents of seven endogenous hormones varied specifically in different development stages of peanuts. This study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit, which are controlled by seven endogenous hormones.
胰岛ß细胞异质性的相关研究进展

胰岛ß细胞在血糖感知和稳态调节中扮演重要角色。研究表明,同一种属不同个体、同一个体不同胰岛间、甚至同一个胰岛内的不同ß细胞在胰腺发育、胰岛结构、细胞形态和功能、分子标志物等方面均存在异质性。ß细胞异质性可能是其能够有效应对各种生理和病理变化的潜在机制之一。充分了解ß细胞异质性将有助于理解糖尿病的发病机制,为糖尿病治疗提供新思路。
Mathematical analysis of organic-pass transistor using pseudo-p-OTFTs

Steady state behavior analysis of organic thin film transistor(OTFTs) has been thoroughly researched in the past few decades. Yet, this static logic analysis has drawbacks of high power dissipation and high power consumption, and a large number of prerequisites in the number of transistors for the digital logic circuit application. Hence, to overcome these basic fundamental drawbacks of static logic, the dynamic logic study of organic thin film transistor has been analyzed in this paper. The fundamental basic of dynamic logic is a pass transistor for which logic high and logic low model is designed at an operating voltage of 5 V and frequency of 5 kHz. Additionally, the novel approach of analytical model for organic pass transistor(OPT) circuit is included and verified using MATLAB. The transient individualities of organic pass transistor OPT are examined through Atlas 2-D numerical device simulator. The reduction in the power dissipation along with additional voltage scaling and reduction in the clock frequency such as pipelining may further enable the applications into more complex VLSI ICs.
An 18-bit sigma –delta switched-capacitor modulator using 4- order single-loop CIFB architecture

Oversampling sigma–delta(Σ–Δ) analog-to-digital converters(ADCs) are currently one of the most widely used architectures for high-resolution ADCs. The rapid development of integrated circuit manufacturing processes has allowed the realization of a high resolution in exchange for speed. Structurally, the Σ–Δ ADC is divided into two parts: a front-end analog modulator and a back-end digital filter. The performance of the front-end analog modulator has a marked influence on the entire Σ–Δ ADC system. In this paper, a 4-order single-loop switched-capacitor modulator with a CIFB(cascade-of-integrators feed-back) structure is proposed. Based on the chosen modulator architecture, the ASIC circuit is implemented using a chartered 0.35 μm CMOS process with a chip area of 1.72 × 0.75 mm~2. The chip operates with a 3.3-V power supply and a power dissipation of 22 mW. According to the results, the performance of the designed modulator has been improved compared with a mature industrial chip and the effective number of bits(ENOB) was almost 18-bit.
A high performance adaptive on-time controlled valley-currentmode DC–DC buck converter

This paper presents an AOT-controlled(adaptive-on-time, AOT) valley-current-mode buck converter for portable application. The buck converter with synchronous rectifier not only uses valley-current-mode control but also possesses hybridmode control functions at the same time. Due to the presence of the zero-current detection circuit, the converter can switch freely between the two operating modes without the need for an external mode selection circuit, which further reduces the design difficulty and chip area. The converter for the application of high power efficiency and wide current range is used to generate the voltage of 0.6–3.0 V with a battery source of 3.3–5.0 V, while the load current range is 0.05–2 A. The circuit can work in continuous conduction mode with constant frequency in high load current range. In addition, a stable output voltage can be obtained with small voltage ripple. In pace with the load current decreases to a critical value, the converter transforms into the discontinuous conduction mode smoothly. As the switching period increases, the switching loss decreases, which can significantly improve the conversion efficiency. The proposed AOT controlled valley current mode buck converter is integrated with standard 0.18 μm process and the simulation results show that the converter provides well-loaded regulations with power efficiency over 95%. When the circuit switches between the two conduction modes drastically, the response time can be controlled within 30 μs. The undershoot voltage is controlled within 25 mV under a large current hopping range.
基于肠道微生态的降糖药物降糖机制

肠道菌群与宿主的能量代谢过程密切相关,其组成和代谢的紊乱可通过多途径导致胰岛素抵抗、肥胖及2型糖尿病的发生。降糖药物可调节肠道菌群的组成及其代谢,改善肠道内环境,从而降低胰岛素抵抗和改善宿主的代谢,最终发挥降糖作用。
Meta-Transcriptome Profiling of Novel Invasive Pest Spodoptera frugiperda in Yunnan, China

The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), a highly agricultural destructive pest of cereals, is originally rampant in tropical and subtropical America (Kenis et al. 2019). The caterpillars of this moth feed on more than 350 plant species (Harrison et al. 2019), including several economically important cultivated grasses such as maize, rice, sorghum and sugarcane, severely threatening the food security (Cock et al. 2017). Spodoptera frugiperda is recognized to comprise two morphologically identical but genetically distinct strains with different host range. The ‘rice strain’(R strain, RS) is thought to preferentially feed on rice and various pasture grasses, while the ‘corn strain’(C strain, CS) on maize, cotton and sorghum (Juarez et al. 2012). Unable to tolerate freezing temperature, S. frugiperda is seldom reported outside of America. However, since its first detection in Nigeria in 2016, several congruent reports from African countries about sudden and severe upsurges of S. frugiperda raised the alarm that S. frugiperda is continuously expanding their range to become truly threat (Goergen et al. 2016). Notably, this pest is now spreading to Southeast Asia countries (including Myanmar and Thailand) and China where the environmental requirements for this pest to establish itself presented (Early et al. 2018). Bordering with Myanmar, Yunnan Province of China is in great danger of S. frugiperda invasion while several sporadic reports have documented the identification of S. frugiperda in Yunnan Province (https://www.ippc.int) (Guo et al. 2018; Zhang et al. 2019). Till now (May, 2019), S. frugiperda has been reported in nearly the whole south region of China where the diverse host plant sources and favorable climatic environment are provided (Kenis et al. 2019). Therefore, it's of great significance to monitor this new pest invasion and take preventive measures to minimize the agricultural damage of it.
综合消肿治疗在头颈部肿瘤淋巴水肿患者中的研究现状

头颈部肿瘤患者在接受手术及放射治疗后有较高风险发生淋巴水肿,综合消肿治疗(complex decongestion therapy,CDT)是临床上淋巴水肿常用的保守治疗方法。本文对CDT在头颈部淋巴水肿患者中的应用内容及效果进行介绍,包括CDT的适应证、主要内容及应用效果,旨在为国内学者开展相关研究或临床实践提供思路。
Ecdysone receptor isoforms play distinct roles in larval-pupal-adult transition in Leptinotarsa decemlineata

A heterodimer of two nuclear receptors,ecdysone receptor (EcR) and ultraspiracle,mediates 20-hydroxyecdysone (20E) signaling to modulate many aspects in insect life,such as molting and metamorphosis,reproduction,diapause and innate immunity.In the present paper,we intended to determine the isoform-specific roles of EcR during larval-pupal-adult transition in the Colorado potato beetle.Double-stranded RNAs (ds-RNAs) were prepared using the common (dsEcR) or isoform-specific (dsEcRA,dsEcRB1) regions of EcR as templates.Ingestion of either dsEcR or dsEcRA,rather than dsEcRB1,by the penultimate (3rd) and final (4th) instar larvae caused failure of larval-pupal and pupal-adult ecdysis.The RNA interference (RNAi) larvae remained as prepupae,or became deformed pupae and adults.Determination of messenger RNA (mRNA) levels of EcR isoforms found that LdEcRA regulates the expression of LdEcRB1.Moreover,silencing the two EcR transcripts,LdEcRA or LdEcRB1 reduced the mRNA levels of Ldspo and Ldsad,and lowered 20E titer.In contrast,the expression levels of HR3,HR4,E74 and E75 were significantly decreased in the LdEcR or LdEcRA RNAi larvae,but not in LdEcRB1 depleted specimens.Dietary supplement with 20E did not restore the expression of five 20E signaling genes (USP,HR3,HR4,E74 and E75),and only partially alleviated the pupation defects in dsEcR-or dsEcRA-fed beetles.These data suggest that EcR plays isoform-specific roles in the regulation of ecdysteroidogenesis and the transduction of 20E signal in L.decemlineata.
Air Temperature and Emersion Time Can Affect the Survival Rate and Ammonium Loading of Swimming Crab Portunus trituberculatus Exposed to Air

Ammonium overloading is a common response of aquatic organisms to air exposure during transport. This study elucidated the relationship between ammonium overloading and mortality of crab Portunus trituberculatus. Additionally, we also explored the effects of emersion time and air temperature on ammonium loading and concomitant physiological change. To test the air temperature effect, the crab was exposed to 16, 23 and 30℃ in air for 3 h, respectively, and then recovered in seawater at 23℃ for 12 h. To test the emersion time effect, crab was exposed to 23℃ in air for 0.5 and 3 h, respectively, and then recovered in seawater at 23℃ for 12 h. In the control group, crab was always immersed at 23℃. At each time interval(0.5, 1.5 and 3 h during air exposure and 0.5, 2,4 and 12 h during recovery), ammonium excretion rate, level of total ammonium, total free amino acids and urea concentration in hemolymph and the hepatopancreas enzyme activity involved in detoxifying ammonium were analysed. Results showed that crab mortality was positively related with emersion time and temperature while ammonium loading was lower at 16 and 30℃ than at 23℃. For crab experiencing thermal inconsistence of culture media(i.e., 16 or 30℃), they were higher in ammonium excretion rate and activities of ammonium detoxification enzymes, which may be the reason that they had a lower ammonium loading. Prolonged emersion time(3.0 h vs. 0.5 h)increased the ammonium overloading and the activity of ammonium detoxification pathways in crab. Our results demonstrated that emersion-induced ammonium overloading may not be the main reason leading to P. trituberculatus death during air exposure and subsequent recovery. When the culture medium changed, thermal variation, compared with constant temperature,could reduce ammonium overloading in crab by elevating the activities of ammonium detoxification enzymes and ammonium excretion rate during recovery period.
Developmental Dynamics of the Larval Muscle System of Bay Scallop(Argopecten irradians)

Though the larval development of bivalves has been extensively studied for commercial purposes, the dynamic development of larval muscle system remains largely unknown. In this study, we characterized the larval muscle system at different developmental stages(D-shaped veligers, umbo veligers and spats)in the bay scallop(Argopecten irradians)by phalloidin staining and under a confocal microscopy. The functional muscles are initially established at the early stage of veligers, which have four pairs of velar retractors and one anterior adductor. At the veliger stage, the velum and posterior retractor muscles are functionally important for velar contractility but undergo an irreversible shrink until they disappear at the end of the larval stage. During metamorphosis, three crucial modifications take place in the larval muscle system. The metamorphosis process involves the gradual degeneration of velum retractors, mantle margin development from an unfolded to a three-fold state, and remodeling of the adductor muscle system from dimyarian(two adductors)to monomyarian condition(one adductor)as in juveniles/adults. All retractor muscles are composed of striated muscle, but both anterior and posterior adductors have smooth and striated components. These findings highlight that the morphological changes at different stages are typical features of myogenesis in scallops. The present knowledge on the developmental dynamics of myogenesis in the bay scallop will not only improve our understanding of phenotypic diversity of larval myoanatomy in bivalves, but also provide useful information on the larval culture in hatcheries.
Impact of Phenanthrene on Denitrification Activity and Transcription of Related Functional Genes in Estuarine and Marine Sediments

The effects of phenanthrene(Phe)on the denitrification activity and denitrifying genes(narG, nirS and nosZ)were evaluated by dose-response experiments in sediments of Dagu River Estuary(DRE)and Jiaozhou Bay(JZB). The results showed that potential denitrification activity(PDA), N_2O, NO_3~- and NO_2~- reduction rates of both areas were inhibited with an increase of Phe concentrations. The PDA, N_2O, NO_3~- and NO_2~- reduction rates of both areas was highest and lowest in the control(DRE: 0.453,0.427, 7.439 and 3.222 mg N kg~(-1) h~(-1), JZB: 0.592, 0.555, 8.470 and 3.793 mg N kg~(-1) h~(-1))and highest Phe amended treatments(DRE: 0.069, 0.001, 4.486, and 1.563 mg N kg~(-1) h~(-1); JZB: 0.114, 0.024, 5.527 and 2.200 mg N kg~(-1) h~(-1)). The inhibition rate of PDA was highest, follow by NO_2~- reduction and then NO_3~- reduction. Moreover, with the increasing of Phe concentrations, total bacteria count and the abundance of denitrifying genes were decreased. And N_2O accumulation was promoted with the addition of Phe for both areas. Based on the comparison of EC_(50) values, denitrifiers harboring three genes were more sensitive to Phe than PDA, and denitrifiers harboring nirS gene were more sensitive, followed by nosZ gene, and then narG gene. Furthermore, according to correlation analysis, the relative abundance of denitrifying genes was much more positively correlated with PDA, NO_3~- and NO_2~- reduction than total bacteria count. In addition, the denitrification activity and total bacteria count in JZB were more inhibited than that of DRE. This study is useful for understanding the impact of Phe pollution on denitrification in estuary and marine sediments, with profound implications for the management of aquatic ecosystems regarding eutrophication(N-removal)and greenhouse effect.
高产优质三系杂交早稻新组合五优85

五优85是衡阳市农业科学研究所以三系不育系五丰A与自育恢复系CR85配组育成的三系杂交早稻新组合。该组合表现优质、高产等特点,2017年5月通过湖南省农作物品种审定委员会审定。
直播晚稻品种的筛选及其丰产性分析

为筛选出适宜赣北地区直播的晚稻品种,以33个晚稻品种为材料,人工模拟机械穴直播,比较分析了其生育期、成穗率、干物质积累动态及产量构成等。结果表明,不同品种的全生育期为105 ~ 120 d,其中黄华占、春优84、天优华占生育期最长(120 d),盛泰优9712和泰丰优208生育期最短(105 d)。不同品种的成穗率有一定的差异,且相关分析表明,直播晚稻的产量与成穗率呈显著正相关。以产量为指标进行聚类分析,将参试的33个水稻品种分为高产、中产和低产3类,其中,兴安香占(常规稻)、银丰优华占、粤王丝苗(常规稻)、万象优双占、春优84、桃优香占为高产类品种,广8优金占、美香占2号、五山丝苗、中香1号、粤晶丝苗、丰美占和恒丰优华占为低产类品种,其余为中产类型。高产类品种分别比中产和低产类品种增产了13.98%和28.57%。对不同产量类型水稻品种的群体质量和产量构成特征分析表明,高产类品种表现为在一定穗数的基础上穗大、总颖花量大、千粒重大、结实率和日产量高、各生育期叶绿素含量高、抽穗后干物质增速快、茎鞘和叶片物质输出及转化率高等特点。试验结果可为赣北地区直播晚稻品种选育和遗传改良提供参考。