mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
不同移栽密度对草果种苗质量的影响

目的:为探明草果两段育苗最适的移栽密度及草果种苗质量标准。方法:通过不同移栽密度对草果子床育苗种苗质量影响的研究,设3个不同的移栽密度,分别是高密30株/m~2、中密22.5株/m~2、低密15株/m~2,采用单因素随机区组试验设计。结论:从移栽后的30 d开始,密度显著地影响草果种苗的植株高度、单株叶面积和单株干物质重;草果子床育苗移栽密度不宜大于22.5株/m~2,且经过子床120 d育苗后的草果种苗成苗质量分别应达到:株高约35.0 cm、单株叶面积约200 cm~2/株、单株干物质重约1.30 g/株范围标准。
镉、铅胁迫对麻疯树种子萌发的影响

采用溶液培养实验研究了不同浓度的镉(Cd~(2+))和铅(Pb~(2+))对麻疯树种子萌发的影响。结果表明,2种重金属同一浓度下,Cd~(2+)、Pb~(2+)对麻疯树胚根伸长抑制率大于茎伸长抑制率;随Cd~(2+)、Pb~(2+)浓度的增大,种子发芽率、发芽势、发芽指数、总体呈下降的趋势。
三七的生物学特性研究进展

三七(Panax notoginseng(Burk.)F.H.Chen)为五加科人参属植物,在我国已有400多年的栽培历史,其作为一种名贵中药,具有化瘀止血、消肿止痛等功效。三七的野生资源至今没有被发现,但是人工栽培的三七却存在很多变异种。本文将从种子特性、形态解剖特性、种苗特性、果实特性、生殖特性、遗传多样性和分子生物学特性等方面对三七的生物学特性展开全面、系统的阐述,从而使大家对三七有更加深刻的认识。
Behavior of Mn~(2+) in perovskite manganites with nominal composition La_(0.6-x)Nd_xSr_(0.1)MnO_3

The fact that there are Mn~(2+) at the A sites in the ABO_3 perovskite phase of manganites with the nominal composition La_(0.6-x)Nd_xSr_(0.1)MnO_3 showed by detailed experimental study and theoretical calculations.The magnetic moments of these Mn~(2+) are antiparallel to those of the Mn ions at the B sites.The content of the Mn~(2+) increases as the average ionic radius,
Synthesis,characterization,and thermostability of bis(2,2,6,6-tetramethyl-3,5-heptanedionato)copper(Ⅱ)

Owing to the adaptability to large scale processing,excellent composition control and film uniformity,the metal-organic chemical vapor deposition（MOCVD） technique is a promising process for high-temperature superconductor YBa2Cu3O7-δ（YBCO） preparation.In this technique,the evaporation characteristics and thermostability of adopted precursors in whole process will decide the quality and reproducible results of YBCO film.In the present report,bis（2,2,6,6-tetramethyl-3,5-heptanedionato）copper（II）（Cu（TMHD）2） was synthesized by the interaction of copper acetate hydrate with TMHD in methanol solution,and its structure was identified by FTIR,1 H NMR,and EI-MS spectroscopy.Subsequently,thermal property and the kinetics of decomposition were systematically investigated by nonisothermal thermogravimetric analysis methods（TGA） at different heating rates in streams of N2,and the average apparent activation energy of evaporation process was evaluated by the Ozawa,Kissinger,and Friedman methods.The possible conversion function was estimated through the Coats-Redfern method to characterize the evaporation patterns and followed a phase boundary reaction mechanism by the contracting area equation with average activation energy of 85.1 kJ·mol~(-1).
Performances of a Balanced Hydraulic Motor with Planetary Gear Train

The current research of a balanced hydraulic motor focuses on the characteristics of the motor with three planet gears.References of a balanced hydraulic motor with more than three planet gears are hardly found.In order to study the characteristics of a balanced hydraulic motor with planetary gear train that includes more than three planet gears,on the basis of analysis of the structure and working principle of a balanced hydraulic motor with planetary gear train,formulas are deduced for calculating the hydraulic motor’s primary performance indexes such as displacement,unit volume displacement,flowrate fluctuation ratio,etc.Influences of the gears’ tooth number on displacement and flowrate characteristics are analyzed.In order to guarantee the reliability of sealing capability,the necessary conditions that tooth number of the sun gear and the planet gears should satisfy are discussed.Selecting large unit volume displacement and small displacement fluctuation ratio as designing objectives,a balanced hydraulic motor with three planet gears and a common gear motor are designed under the conditions of same displacement,tooth addendum coefficien and clearance coefficient.By comparing the unit volume displacement and fluctuation ratio of the two motors,it can be seen that the balanced hydraulic motor with planetary gear train has the advantages of smaller fluctuation ratio and larger unit volume displacement.The results provide theoretical basis for choosing gear tooth-number of this kind of hydraulic motor.
Motion Error Compensation of Multi-legged Walking Robots

Existing errors in the structure and kinematic parameters of multi-legged walking robots,the motion trajectory of robot will diverge from the ideal sports requirements in movement.Since the existing error compensation is usually used for control compensation of manipulator arm,the error compensation of multi-legged robots has seldom been explored.In order to reduce the kinematic error of robots,a motion error compensation method based on the feedforward for multi-legged mobile robots is proposed to improve motion precision of a mobile robot.The locus error of a robot body is measured,when robot moves along a given track.Error of driven joint variables is obtained by error calculation model in terms of the locus error of robot body.Error value is used to compensate driven joint variables and modify control model of robot,which can drive the robots following control model modified.The model of the relation between robot’s locus errors and kinematic variables errors is set up to achieve the kinematic error compensation.On the basis of the inverse kinematics of a multi-legged walking robot,the relation between error of the motion trajectory and driven joint variables of robots is discussed.Moreover,the equation set is obtained,which expresses relation among error of driven joint variables,structure parameters and error of robot’s locus.Take MiniQuad as an example,when the robot MiniQuad moves following beeline tread,motion error compensation is studied.The actual locus errors of the robot body are measured before and after compensation in the test.According to the test,variations of the actual coordinate value of the robot centroid in x-direction and z-direction are reduced more than one time.The kinematic errors of robot body are reduced effectively by the use of the motion error compensation method based on the feedforward.
Fault diagnosis and isolation of the componentand sensor for aircraft engine

Aircraft engine component and sensor fault detection and isolation approach was proposed,which included fault type detection module and component-sensor simultaneous fault isolation module.The approach can not only distinguish among sensor fault,component fault and component-sensor simultaneous fault,but also isolate and locate sensor fault and the type of engine component fault when the engine component fault and the sensor faults occur simultaneously.The double-threshold mechanism has been proposed,in which the fault diagnostic threshold changed with the sensor type and the engine condition,and it greatly improved the accuracy and robustness of sensor fault diagnosis system.Simulation results show that the approach proposed can diagnose and isolate the sensor and engine component fault with improved accuracy.It effectively improves the fault diagnosis ability of aircraft engine.
澳大利亚促进科研成果产业化的一些措施值得借鉴

提高科研水平是实施科教兴国和人才强国战略的一个重要组成部分。提高科研的投入产出比能使有限的科研经费真正落到实处。在中国的科研经费投入还不如欧美的情况下，提高效益应该是一个最佳选择。
Construction of Two Suppression Subtractive Hybridization Libraries and Identification of Salt-Induced Genes in Soybean

Soybean is planted worldwide and its productivity is significantly hampered by salinity. Development of salt tolerant cultivars is necessary for promoting soybean production. Despite wealth of information generated on salt tolerance mechanism, its basics still remain elusive. A continued effort is needed to understand the salt tolerance mechanism in soybean using suitable molecular tools. To better understand the molecular basis of the responses of soybean to salt stress and to get an enrichment of critical salt stress responsive genes in soybean, suppression subtractive hybridization libraries (SSH) are constructed for the root tissue of two cultivated soybean genotypes, one was tolerant and the other was sensitive to salt stress. To compare the responses of plants in salt treatment and non-treatment, SSH1 was constructed for the salt-tolerant cultivar Wenfeng 7 and SSH2 was constructed for the salt-sensitive cultivar Union. From the two SSH cDNA libraries, a total of 379 high quality ESTs were obtained. These ESTs were then annotated by performing sequence similarity searches against the NCBI nr (National Center for Biotechnology Information protein non-redundant) database using the BLASTX program. Sixty-three genes from SSH1 and 49 genes from SSH2 could be assigned putative function. On the other hand, 25 ESTs of SSH1 which may be not the salt tolerance-related genes were removed by comparing and analyzing the ESTs from the two SSH libraries, which increased the proportion of the genes related to salt tolerance in SSH1. These results suggested that the novel way could realize low background of SSH and high level enrichment of target cDNAs to some extent.
药房新模式服务新探索

近年来,中老年病人的高血压、糖尿病、高脂血症、脑卒中、癌症、乙型肝炎等慢性病的发病率逐年上升,通过药物治疗来控制这些疾病的发展,改善病人的生活质量显得越来越重要[1]。上海市闸北区彭浦新村街道社区卫生服务中心是一所一级甲等医院,随着医改的不断深入以及本中心优秀人才的引进,越来越多的病人前来本中心就诊,2010年门诊量累计达65万多人次,日最高门诊量接近4000人次。门诊药房作为医院工作的一线窗口，是为病人提供医疗服务的重要部门。围绕着将工作从“以药物为中心”转为“以病人为中心”这一重点[1]，本中心领导、科领导及工作人员结合实际情况，着眼于降低差错率、简化手续、缩短病人等候时间、增加病人对药品的了解等方面开展了一系列工作。为此，本中心门诊药房的运行模式也做了调整，最大程度上为病人服务。
中老年男性下尿路症状与良性前列腺增生之间的相关性

下尿路症状(LUTS)与良性前列腺增生(BPH)在中老年男性中发病率较高。研究表明50岁以上男性中出现LUTS人数约占总人群的25%，严重降低中老年男性生活质量和心理健康。LUTS与BPH以往常被认为是长期共存的状态。现阶段研究也证实二者存在一定联系。本研究通过分析老年男性BPH患者临床资料，探讨LUTS与BPH之间的相关性。
比索洛尔对老年冠心病患者QT离散度的影响

目的 探讨β-受体阻断剂比索洛尔(Bisoprolol)对老年冠心病患者QT离散度(QTd)的影响。方法 采用单盲处理的方法，观察48例老年冠心病患者比索洛尔治疗前后的最大QT间期(QTmax)、最小QT间期(QTmin)、心率校正QT间期离散度(QTcd)、QTd及RR间期的变化。结果 观察组Bisoprolol治疗后冠心病患者QTmin延长〔(390±32.7)ms vs(357±21.2)ms，P<0.01〕，而QTmax无明显改变〔(428±34.7)ms vs(427±33.6)ms，P>0.05〕，QTd、QTcd则显著缩小〔分别为(39±11.8)ms vs(72±28.6)ms，(42±10.7)ms vs(78±24.6)ms，均P<0.01〕。对照组治疗前后QTd、QTcd均无改变(P>0.05)。结论 Bisoprolol治疗恶性心律失常的可能机制是通过延长冠心病患者的QTmin，缩小心肌复极化离散度，促使心肌细胞复极化趋向同步进行，此种机制有利于防止冠心病患者恶性室性心律失常的发生。
清热止痛方内服和外敷治疗老年急性痛风性关节炎临床观察

对于痛风性关节炎,现代医学目前主要采用秋水仙碱、非甾体类抗炎药治疗,但秋水仙碱有严重的胃肠道反应、肾功能损害、骨髓抑制等不良反应,非甾体类抗炎药虽有消炎止痛效果,也有消化道反应、头痛及过敏反应等,临床应用常受到限制。笔者运用中药内服和外敷治疗老年急性痛风性关节炎患者取得较好疗效,且不良反应少,现总结报道如下。
六神丸联合卡介菌多糖核酸治疗老年人急性带状疱疹疗效观察

目的探讨六神丸联合卡介菌多糖核酸注射液治疗老年人急性带状疱疹的临床疗效。方法122例带状疱疹患者随机分为两组,每组均给予阿昔洛韦片400 mg、呋喃硫胺片25 mg,每日3次,口服作为基础治疗。治疗组以六神丸碾碎加水或米醋外涂患处每日3次,卡介菌多糖核酸注射液1 mg隔日肌注。对照组以炉甘石洗剂外涂患处每日3次。两组均连续用药2周,治疗结束后评价临床疗效。结果治疗组止痛时间、止疱时间、结痂时间较对照组明显缩短。治疗组有效率为100%,对照组为88.5%。两组比较差异有统计学意义(P<0.05)。结论六神丸联合卡介苗菌多糖核酸治疗老年人带状疱疹疗效确切。
桐花树多糖提取及其抑菌活性研究

目的提取桐花树多糖并对其抑菌活性进行研究。方法用水提醇沉法提取桐花树粗多糖,利用Sephadex G-200柱和DEAE-52纤维素柱对粗多糖进行分离纯化,用滤纸片法研究桐花树多糖及其分离组分的抑菌能力。结果分离出桐花树多糖-Ⅰ和桐花树多糖-Ⅱ。桐花树多糖对大肠杆菌、枯草芽孢杆菌、黑曲霉菌有抑菌作用,MIC值为2 mg/mL、2 mg/mL、4 mg/mL。桐花树多糖Ⅰ对大肠杆菌、枯草芽孢杆菌有抑菌作用,MIC值为1 mg/mL与4 mg/mL。桐花树多糖Ⅱ对大肠杆菌、黑曲霉菌有抑菌作用,MIC值为4 mg/mL与8 mg/mL。结论桐花树多糖对大肠杆菌、枯草芽孢杆菌、黑曲霉菌有抑菌活性。
基于主成分分析的中药粉体流动性表征研究

目的采用主成分分析法表征中药粉体的流动性。方法使用流动性较好的直压辅料与中药粉体为原料,对物料作粉体的综合特征测试,采用主成分分析法对中药粉体的流动性进行评价和分析,同时与Carr指数加权法进行比较。结果使用主成分分析法对中药粉体的流动性进行评价是可行的。结论可以根据实际情况确定粉体流动性临界值,同时具备量化的数值,改变了过去单独依赖休止角表征中药粉体的流动性的方法,对于评价粉体流动性形成了相对客观的标准。
石芎挥发油亚微乳的制备工艺和稳定性研究

目的制备石芎挥发油亚微乳,并对其稳定性进行考察。方法对石芎挥发油亚微乳的处方和制备工艺进行研究,分别采用正交试验、单因素试验对处方和制备工艺进行优化。结果优化处方和制备工艺条件为:石芎挥发油10 g、大豆油5 g、油酸0.7 mL、磷脂1.2 g、甘油2.2 g、泊洛沙姆188(F-68)1.2 g,加水至100 mL,以80 MPa的压力高压乳匀6次。结论所制亚微乳处方合理,样品符合《中国药典》中有关亚微乳的质量标准,样品稳定。
恶性肿瘤患儿预防感染的流程改进

儿童恶性肿瘤严重威胁儿童的身体健康,是儿童死亡的主要原因之一。疾病本身及化疗药物引起的免疫功能下降导致肿瘤患儿对各种病原的易感性增高,肿瘤化疗患者最易并发感染的部位是呼吸道、皮肤、黏膜、泌尿系、肛周区域和口腔[1]。我科在循证护理的基础上进行流程再造与优化,从而降低恶性肿瘤患儿感染风险。
两种不同成分培养基制备CIK细胞的生物学特征

目的:采用细胞因子诱导的杀伤细胞(cytokine induced killer cell,CIK cell)培养基(含低浓度IL-2、IFN-γ、IL-12和anti-CD3)和NK培养基(含高浓度IL-2和anti-CD3)体外刺激诱导CIK,分析CIK细胞的扩增、表型和细胞因子分泌情况。方法:健康自愿者来源的外周血单个核细胞(peripheral blood mononuclear cell,PBMC),分别加入CIK培养基和NK培养基进行诱导培养,24 h后,换用含IL-2的1%自体血浆培养基继续诱导培养2周。在培养的第6天和第10天检测培养上清中IFN-γ的分泌情况,在培养的第10天以流式细胞术检测两组培养细胞表面CD3、CD4、CD8、CD16、CD56的表达情况。结果:CIK培养基和NK培养基均可使培养细胞在第7天时开始明显扩增,第10天时可扩增至200倍。其中,CIK培养基获得的细胞扩增倍数明显高于NK培养基,其IFN-γ分泌明显高于NK培养基组[(724.7±434.8)vs(108.9±60.6)pg/ml,P<0.01];而以NK培养基培养的细胞,CD3~-CD16~+CD56~+细胞比例高于CIK培养基[(6.27±3.33)%vs(2.88±1.88)%,P<0.05]。结论:不同成分的CIK培养基和NK培养基均可促进CIK细胞的扩增和成熟,但呈现不同的生物学特征。
Sexual selection and magic traits in speciation with gene flow

The extent to which sexual selection is involved in speciation with gene flow remains an open question and the subject of much research. Here, we propose that some insight can be gained from considering the concept of magic traits (i.e., traits involved in both reproductive isolation and ecological divergence). Both magic traits and other, "non-magic", traits can contribute to speciation via a number of specific mechanisms. We argue that many of these mechanisms are likely to differ widely in the extent to which they involve sexual selection. Furthermore, in some cases where sexual selection is present, it may be prone to inhibit rather than drive speciation. Finally, there are a priori reasons to believe that certain categories of traits are much more effective than others in driving speciation. The combination of these points suggests a classification of traits that may shed light on the broader role of sexual selection in speciation with gene flow. In particular, we suggest that sexual selection can act as a driver of speciation in some scenarios, but may play a negligible role in potentially common categories of magic traits, and may be likely to inhibit speciation in common categories of non-magic traits [Current Zoology 58 (3): 507-513, 2012].
Analyses towards determining Madagascar's place in global biogeography

The relationships of Madagascan plant and animal taxa have been the object of much fascination, Madagascar sharing numerous lineages with Africa, others with Asia, Australia, or the Americas, and many others being of uncertain relationships. In commonly accepted global regionalization schemata, Madagascar is treated together with Africa for animals, and with Africa, tropical Asia and the Pacific islands in the case of plants. Here we examine the similarities between the biotic assemblages of (i) tropical Africa, (ii) Madagascar, and (iii) the rest of the world, on a basic taxonomic level, considering the families of vascular plants and vertebrates as analysis units. The percentages of endemic families, families shared pair-wise between regions, or present in all three, are roughly similar between the two broad groups, though plant families with ranges limited to one region are proportionally fewer. In dendrograms and multidimensional scaling plots for different groups, Madagascar clusters together with Africa, Asia or both, and sometimes with smaller Indian Ocean Islands, but quite often (though not in plants) as a convincingly separate cluster. Our results for vertebrates justify the status of full zoogeographic region for Madagascar, though an equally high rank in geobotanical regionalization would mean also treating Africa and Tropical Asia as separate units, which would be debatable given the overall greater uniformity of plant assemblages. Beyond the Madagascan focus of this paper, the differences between plant and vertebrate clusters shown here suggest different levels of ecological plasticity at the same taxonomic level, with plant families being much more environmentally-bound, and thus clustering along biome lines rather than regional lines [Current Zoology 58 (3): 363-374, 2012].
Magnetic anisotropy and magnetization reversal of ultrathin iron films with in-plane magnetization on Si(111) substrates

The magnetic anisotropy and magnetization reversal of single crystal Fe films with thickness of 45 monolayer (ML) grown on Si(111) have been investigated by ferromagnetic resonance (FMR) and vibrating sample magnetometer (VSM). Owing to the significant modification of the energy surface in remanent state by slight misorientation from (111) plane and a uniaxial magnetic anisotropy, the azimuthal angular dependence of in-plane resonance field shows a six-fold symmetry with a weak uniaxial contribution, while the remanence of hysteresis loops displays a two-fold one. The competition between the first and second magnetocrystalline anisotropies may result in the switching of in-plane easy axis of the system. Combining the FMR and VSM measurements, the magnetization reversal mechanism has also been determined.
Ultraviolet and mid-infrared continuum generation by cross-phase modulation between red-shifted solitons and blue-shifted dispersive waves in a photonic crystal fiber

Using a photonic crystal fiber with a zero dispersion wavelength of the fundamental mode at 780 nm designed and fabricated in our lab, the ultraviolet and mid-infrared continua are generated by cross-phase modulation between red-shift solitons and blue-shift dispersive waves. The dependences of continuum on the pump power and wavelength are investigated. With the pump working at 820 nm, when the pump power increases from 300 to 500 mW, the bandwidths of ultraviolet and mid-infrared continua change from 80 to 140 nm and 100 to 200 nm, respectively. The wavelength of ultraviolet continuum is below 246 nm, and the wavelength of mid-infrared continuum exceeds 2500 nm. Moreover, the influences of pump power on wavelength and conversion efficiency of different parts of continua are also demonstrated.
Monte Carlo study of the universal area distribution of clusters in the honeycomb O(n) loop model

We investigate the area distribution of clusters (loops) in the honeycomb O(n) loop model by means of the worm algorithm with n = 0.5, 1, 1.5, and 2. At the critical point, the number of clusters, whose enclosed area is greater than A, is proportional to A~(-1) with a proportionality constant C. We confirm numerically that C is universal, and its value agrees well with the predictions based on the Coulomb gas method.
双酚A对大豆幼苗叶绿素荧光参数的影响

双酚A(Bisphenol A,BPA)是酚类化合物中具有代表性的环境污染物之一,具有广泛存在性与潜在致癌及生殖毒性。迄今为止,BPA对植物生育及生理影响报道较少。叶绿素荧光参数可表征植物光合作用光反应对光能吸收、传递、耗散、分配等特征。本文以大豆(Glycinemax)幼苗为试材,选择BPA饮用水安全值(1.5mg·L~(-1))、垃圾渗滤液最大浓度(17.2mg·L~(-1))等2个剂量胁迫大豆幼苗7d,对照与各处理组均3盆,每盆3株,3次重复。处理第7d与14d时取样,采用叶绿素荧光测定仪(PM2000,Germany)测定植株叶绿素荧光参数Fm(最大荧光产量），qP（光化学淬灭系数），qN（非光化学淬灭系数）对BPA的响应。
Solar cell performance improvement via photoluminescence conversion of Si nanoparticles

Photoluminescence (PL) conversion of Si nanoparticles by absorbing ultraviolet (UV) lights and emitting visible ones has been used to improve the efficiency of crystalline Si solar cells. Si nanoparticle thin films are prepared by pulverizing porous Si in ethanol and then mixing the suspension with a SiO2 sol-gel (SOG).This SOG is spin-deposited onto the surface of the Si solar cells and dries in air. The short-circuit current as a function of Si nanoparticle concentration is investigated under UV illumination. The maximal increase is found at a Si concentration of 0.1 mg/mL. At such concentration and under the irradiation of an AM0 solar simulator, the photoelectric conversion efficiency of the crystalline Si solar cell is relatively increased by 2.16% because of the PL conversion.
Test and analysis of the halo in low-light-levelimage intensifiers

A halo tester is designed to analyze the halo formation in low-light-level (LLL) image intensifiers and the influencing factors on halo size. The tester is used to collect a 0.1922-mm hole image directly using a CoolSNAPK4 charge-coupled device (CCD) in a darkroom under illumination conditions between 10~(-2)and 10~(-4) lx. The practical measurement result shows that the amplification ratio is 343.4. Then, the super second- and third-generation image intensifiers are placed after the hole, and the halo sizes of the hole images on the screens are determined as 0.2388 and 0.5533 mm, respectively.
Tissue cholesterol content alterations in streptozotocin-induced diabetic rats

Aim: Diabetes is associated with elevated serum total cholesterol level and disrupted lipoprotein subfractions. The aim of this study was to examine alterations in the tissue cholesterol contents closely related to diabetic complications. Methods: Intraperitoneal injection of streptozotocin was used to induce type 1 diabetes in adult male Sprague-Dawley rats. On d 35 after the injection, liver, heart, intestine, kidney, pancreas, cerebral cortex and hippocampus were isolated from the rats. The content of total and free cholesterol in the tissues was determined using HPLC. The ATP-binding cassette protein A1 (ABCA1) protein and ApoE mRNA were measured using Western blot and QT-PCR analyses, respectively. Results: In diabetic rats, the level of free cholesterol was significantly decreased in the peripheral tissues, but significantly elevated in hippocampus, as compared with those in the control rats. Diabetic rats showed a trend of decreasing the total cholesterol level in the peripheral tissues, but significant change was only found in kidney and liver. In diabetic rats, the level of the ABCA1 protein was significantly increased in the peripheral tissues and cerebral cortex; the expression of ApoE mRNA was slightly decreased in hippocampus and cerebral cortex, but the change had no statistical significance. Conclusion: Type 1 diabetes decreases the free cholesterol content in the peripheral tissues and increases the free cholesterol content in hippocampus. The decreased free cholesterol level in the peripheral tissues may be partly due to the increased expression of the ABCA1 protein.
Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit in vitro

Aim: To examine the effects of anisomycin on glioma cells and the related mechanisms in vitro. Methods: The U251 and U87 human glioblastoma cell lines were tested. The growth of the cells was analyzed using a CCK-8 cell viability assay. Apoptosis was detected using a flow cytometry assay. The expression of proteins and phosphorylated kinases was detected using Western blotting. Results: Treatment of U251 and U87 cells with anisomycin (0.01-8 μmol/L) inhibited the cell growth in time- and concentration-dependent manners (the IC50 values at 48 h were 0.233±0.021 and 0.192±0.018 μmol/L, respectively). Anisomycin (4 μmol/L) caused 21.5%±2.2% and 25.3%±3.1% of apoptosis proportion, respectively, in U251 and U87 cells. In the two cell lines, anisomycin (4 μmol/L) activated p38 MAPK and JNK, and inactivated ERK1/2. However, neither the p38 MAPK inhibitor SB203580 (10 μmol/L) nor the JNK inhibitor SP600125 (10 μmol/L) prevented anisomycin-induced cell death. On the other hand, anisomycin (4 μmol/L) reduced the level of PP2A/C subunit (catalytic subunit) in a time-dependent manner in the two cell lines. Treatment of the two cell lines with the PP2A inhibitor okadaic acid (100 nmol/L) caused marked cell death. Conclusion: Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit. The regulation of PP2A/C exression by anisomycin provides a clue to further study on its role in glioma therapy.
大黄对急性胰腺炎患者MDA、SOD的影响及疗效观察

急性胰腺炎(acute pancreatitis,AP)是由于多种原因引起的胰腺自身消化性疾病,随着疾病的进展可导致局部和全身并发症,甚至多脏器功能衰竭,其中氧化应激在AP的发病过程中起重要作用~([1])。有报道应用维生素C、维生素E、N-乙酰半胱氨酸等抗氧化治疗能减轻细胞损伤,有效改善AP患者的症状,并降低病死率~([2~4])。多年来我院应用生大黄治疗AP取得较好的临床疗效。本文观察了生大黄对AP患者SOD、MDA的影响。现报道如下
丹参酮ⅡA对房颤大鼠外周血肿瘤坏死因子α表达的影响

目的通过探讨丹参酮ⅡA(TanⅡA)对房颤(AF)大鼠外周血肿瘤坏死因子α(TNF-α)表达的影响,揭示活血祛瘀中药预防和治疗房颤的作用机制。方法采用乙酰胆碱-氯化钙(Ach-Cacl2)药物经尾静脉注射法复制AF大鼠模型,随机分成3组:模型组、丹参酮ⅡA组、维拉帕米干预组。并另设正常对照组。用药两周后,观察丹参酮ⅡA对房颤大鼠外周血TNF-α表达的影响。结果与正常对照组相比,模型组的TNF-α水平明显增高(P<0.01);与模型组相比,丹参酮ⅡA组及维拉帕米干预组的TNF-α水平降低(P<0.01),丹参酮ⅡA组降低更明显。结论丹参酮ⅡA能够明显降低血清TNF-α的水平,可能通过抑制炎症因子的表达,改善房颤心房组织损伤,从而治疗房颤。
富铬酵母对实验性糖尿病大鼠肠系膜微循环的影响

目的观察富铬酵母对实验性糖尿病大鼠肠系膜微循环的影响,探讨有机铬对糖尿病作用的机理。方法用链脲佐菌素诱导复制实验性糖尿病大鼠模型,分别富铬酵母和二甲双胍进行干预,检测各组大鼠血糖、微循环的变化。结果成功建立糖尿病大鼠动物模型。富铬酵母治疗组与糖尿病对照组比较,富铬酵母治疗组血糖下降,大鼠肠系膜微血管直径、微动脉流速差异有统计学意义(P<0.05);血液渗出明显减少,差异有统计学意义(P<0.01)。富铬酵母治疗组与二甲双胍组比较,两组大鼠肠系膜微血管直径、微动脉流速及血液渗出差异均无统计学意义(P>0.05)。结论富铬酵母可以明显降低大鼠的血糖水平和改善肠系膜微循环状态,富铬酵母可以作为治疗糖尿病的辅助性用药。
青蟹水煎液对小鼠妊娠影响的实验研究

目的研究广西青蟹水煎液对小鼠妊娠的影响。方法随机将40只妊娠小鼠分成5组,即A组:生理盐水组;B组:米非司酮组;C、D、E组分别为青蟹低、中、高剂量组,每组8只,灌胃给药。剂量分别为:A组给予生理盐水0.2 ml/10g;B组给予米非司酮2 mg/kg;C、D、E组分别给予青蟹水煎液5,10,20 g/kg。结果青蟹水提物5,10,20 g/kg对早期妊振均有较显著的致流产、致死胎的作用,且随剂量增加作用增强,部分胚胎坏死吸收。结论青蟹主要影响小鼠妊娠着床期和孕早期,对妊娠中晚期影响不大。