mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Propofol Affects Different Human Brain Regions Depending on Depth of Sedation

Objective To investigate the effect of propofol on brain regions at different sedation levels and the association between changes in brain region activity and loss of consciousness using blood oxygen level-dependent functional magnetic resonance imaging (BOLD-fMRI) and bispectral index (BIS) monitoring. Methods Forty-eight participants were enrolled at Peking Union Medical College Hospital from October 2011 to March 2012 and randomly assigned to a mild or a deep sedation group using computer-generated random numbers. Preliminary tests were performed a week prior to scanning to determine target effect site concentrations based on BIS and concomitant Observer's Assessment of Alertness/Sedation scores while under propofol. Within one week of the preliminary tests where propofol dose-response was established, BOLD-fMRI was conducted to examine brain activation with the subject awake, and with propofol infusion at the sedation level. Results Mild propofol sedation inhibited left inferior parietal lobe activation. Deep sedation inhibited activation of the left insula, left superior temporal gyrus, and right middle temporal gyrus. Compared with mild sedation, deep propofol sedation inhibited activation of the left thalamus, precentral gyrus, anterior cingulate, and right basal nuclei. Conclusion Mild and deep propofol sedation are associated with inhibition of different brain regions, possibly explaining differences in the respective loss of consciousness processes.
疣状皮肤结核2例

疣状皮肤结核常见于暴露部位,初起为黄豆大小紫红丘疹,质硬,玻片压诊无苹果酱现象。丘疹逐渐向周围扩大,变成斑块,质硬,以后呈疣状增生,呈暗紫色,上覆以结痂、鳞屑,外围为平滑红晕区,病程慢性,多年不愈。痊愈时损害中央先红,疣状增生逐渐变平,鳞痂脱落,留有光滑柔软而表浅瘢痕。疣状皮肤结核发病前多有外伤史,本例患者起病后多次误诊为股癣,故应与北美芽生菌病、孢子丝菌病等真菌病相鉴别。本病发生率低,临床表现多样,易误诊,还需与汗孔角化症,环状肉芽肿等鉴别。诊断主要依据病史,皮损形态,结核病接触史,组织病理检查和对抗结核治疗反应,追问病史,患者既往曾常口服伊曲康唑胶囊,及外用多种抗真菌药物治疗,但均无效。例1治疗7个月后,例2治疗4个月后,疗效都不显著,但还是有改善。例1常规治疗9个月后,例2常规治疗6个月后,皮损均已痊愈。该2例患者临床表现类似于股癣,所以多次误诊,也曾在当地医院口服及外用抗真菌药物治疗,均无效,故暂可排除真菌感染。实验室多次真菌检查阴性,虽然不能完全排除真菌感染,但病理检查上支持皮肤结核诊断,且按皮肤结核治疗痊愈,故本诊断明确。在临床工作中,对类似病例,在排除真菌感染后,仍不能确诊者,行结核菌素试验及病理检查以早日明确诊断,对症治疗,缩短病程,提高疗效,减轻患者痛苦。
The impact of macroalgae on mean and turbulent flow fields

In this paper, we attempt to quantify the mean and turbulent flow fields around live macroalgae within a tidal inlet in Norway. Two Laminaria digitata specimens 0.50m:apart were selected for detailed study and a profiling ADV was used to collect 45 velocity profiles, each composed of up to seven 0.035 m-high profiles collected for 240 s at 100 Hz, at a streamwise spacing of 0.25 m and cross-stream spacing of 0.20 m. To quantify the impact of the macroalgae, measurements were repeated over a sparser grid after the region had been completely cleared of algae and major roughness elements.
Thermodynamic analysis for a third grade fluid through a vertical channel with internal heat generation

This paper makes the thermodynamic analysis in forced convective flow of a third grade fluid through a vertical channel. Due to the reactive nature of the fluid, the effect of internal heat generation is considered and assumed to be a linear function of temperature. The coupled nonlinear dimensionless ordinary differential equations governing the fluid flow are solved by using the Adomian decomposition method (ADM). The effects of various physical parameters such as third grade material parameter, buoyancy parameter and heat generation parameter on the thermal structure of flow are presented and discussed.
Improvement of predictions of petrophysical transport behavior using three-dimensional finite volume element model with micro-CT images

Due to the intricate structure of porous media, the macroscopic petrophysical transport properties such as the permeability and the saturation used for the reservoir prediction also show a very complex nature and are difficult to obtain. Thus, a better understanding of the influence of the rock structure on the petrophysical transport properties is important. In this paper, we present a universal finite volume element modeling approach to reconstruct the three dimensional pore models from the micro-CT images based on the commercial software Mimics and ICEM, prior to the pore network model based on some basic assumptions. Moreover, tetra finite volume elements are piled up to realize the geometry reconstruction and the meshing process. Compared with the former methods, this process avoids the tremendously large storage requirement for the reconstructed porous geometry and the failures of meshing these complex polygon geometries, and at the same time improves the predictions of petrophysical transport behaviors. The model is tested on two Berea sandstones, four sandstone samples, two carbonate samples, and one Synthetic Silica. Single- and two phase flow simulations are conducted based on the Navier-Stokes equations in the Fluent software. Good agreements are obtained on both the network structures and predicted single- and two- phase transport properties against benchmark experimental data.
Numerical simulation of turbulent flow behind sluice gate under submerged discharge conditions

The turbulent flow discharging under a sluice gate is one kind of typical flows with complicated boundaries in hydraulic and hydroelectric engineering, and the characteristics of its flow field and flow evolution are important in engineering hydraulics. Although there were many studies in this regard, which mainly focus on the discharge capacity, the local scour and the mean flow field, some issues remain to be further investigated, for example, the variation of the mechanical energy during the scouring process, and the adjustment of the unsteady turbulent flow after the local scour with the downstream steady uniform flow. In this paper, the turbulent flow behind a sluice gate is divided into a rapidly varied flow region and a gradually varied flow region, and the above problems are investigated by theoretical analysis and numerical simulation. The main conclusions are as follows:(1) In the discharging process of the turbulent flow under a sluice gate, the river bed would be scoured continuously and the water–air interface is adjusted accordingly, which leads to a decrease of the mechanical energy of the total flow in the rapidly varied flow region, and the mechanical energy loss would also decrease with the increase of the time, yet the variation of the flow discharge under the sluice gate is very small. (2) In the link between the rapidly varied flow and the uniform flow downstream, the mean wall shear stress and the coefficient for the mechanical energy loss would decrease in the longitudinal direction, the decay of the turbulent kinetic energy near the free surface is much more significant than that near the bed in the longitudinal direction, and the mean turbulent kinetic energy in the section would decrease in the longitudinal direction and the decreasing rate is smaller than that of the mean wall shear stress.
Flow field simulation of supercritical carbon dioxide jet:Comparison and sensitivity analysis

As a new jet technology developed in recent years, the supercritical carbon dioxide (SC-CO_2) jet technology enjoys many advantages when applied in oil and gas explorations. In order to study the properties and parametric influences of the SC-CO_2 jet, the flow fields of the SC-CO_2 jet are simulated using the computational fluid dynamics method. The flow field of the SC-CO_2 is compared with that of the water jet. The influences of several parameters on the flow field of the SC-CO_2 jet are studied. It is indicated that like the water jet, the velocity and the pressure of the SC-CO_2 jet could be converted to each other, and the SC-CO_2 jet can generate a significant impact pressure on the wall, the SC-CO_2 jet has a stronger impact pressure and a higher velocity than those of the water jet under the same conditions, the maximum velocity and the impact pressure of the SC-CO_2 jet increase with the increase of the nozzle pressure drop, under the stimulation condition of this study, the influence of the SC-CO_2 temperature on the impact pressure can be neglected in engineering applications, while the maximum velocity of the SC-CO_2 jet increases with the increase of the fluid temperature. This paper theoretically explores the properties of the SC-CO_2 jet flow field, and the results might provide a theoretical basis for the application of the SC-CO_2 jet in oil and gas well drillings and fracturing stimulations.
构建国家自然科学基金委员会-地方政府联合基金项目管理制度探析

本文以国家自然科学基金委员会与地方政府共同成立的“联合基金”(NSFC-地方政府联合基金)为研究对象,从制度管理的角度,探讨该专项制度构建的理论依据及关键内容,重点研究为NSFC-地方政府联合基金创新发展提供保障的制度。
尖晶石型锰氧化物的设计、制备及电催化性能研究

氢氧燃料电池和金属空气电池以空气中的氧气为阴极活性物质,具有理论能量密度高、绿色环保等优点,作为电化学能量储存与转化装置和技术,应用潜力巨大。发生在这两种电池体系阴极上的氧还原反应存在过电位高、动力学缓慢等问题,是性能的关键制约因素之一,因此需要使用阴极催化剂。铂等贵金属及其合金是目前综合性能最佳的氧还原催化剂,但价格昂贵和资源有限,限制了规模实际应用,开发非贵金属基催化材料成为研究热点。3d电子过渡金属氧化物例如锰氧化物拥有储量丰富和环境友好等优点,作为电催化材料引起广泛兴趣;研究表明,锰氧化物的氧还原催化性能与其组成、晶型、价态、形貌、尺寸等因素密切关联。本课题组围绕二元锰氧化物如MnO_2和Mn_2O_3开展了系列研究,制备了纳米颗粒、纳米线、纳米棒、纳米管、微球等不同形态的锰氧化物,揭示了晶相和微结构的重要影响,并发展了氢化、引入氧缺陷等途径提高催化活性。然而,由于电导率低、吸附解离氧能力较差且催化活性位少,含单一金属元素的锰氧化物对氧还原反应的电催化性能仍然有待提高。含多种金属元素的复合锰氧化物例如具有尖晶石(AB_2O_4)和钙钛矿(ABO_3)结构的锰基化合物受到关注。揭示这类复合锰氧化物电催化的构效关系进而指导廉价、高效电催化材料的制备与设计仍然是有意义而具挑战性的课题。我们进一步以尖晶石型锰氧化物为研究对象,发展了“还原—转晶”以及“氧化沉淀—嵌入晶化”两种节能减排、操作简单的制备新策略,在温和条件下实现了系列锰系尖晶石氧化物的组成、晶相、形貌的调控,发现并解释了钴锰尖晶石性能与晶型和钴锰比例的对应规律,获得了具有小尺寸、大比表面积、高催化活性的钴锰氧尖晶石纳米材料。研究成果为氢氧燃料电池与金属空气电池中金属氧化物替代贵金属铂催化剂的设计提供了依据。
肾集合管癌合并肾管状囊性癌一例报告

本例患者术前CT平扫及增强与典型的肾集合管癌和肾管状囊性癌不符,术前诊断为左肾重度积水。分析原因有:①患者有左肾切开取石术史,手术对周围组织及肾的解剖结构会造成一定的影响,可能影响肿瘤的CT影像学表现。②患者左肾重度积水,且其余各项检查均未提示肿瘤性病变。③这两种肿瘤发生率低,我们对其影像学特点认识不足。本例患者行根治性肾切除,由于术中粘连,实际上是包膜内切除,并行肾门旁软组织清除。术后随访7个月,因肿瘤原位复发而死亡。
巨噬细胞在肾钙化斑介导草酸钙结石形成中的调控作用研究进展

泌尿系结石中80%以上的结石成分是草酸钙。草酸钙肾结石的形成机制极其复杂,迄今尚未完全明了。目前,以草酸钙为主要成分的肾结石患者治愈后的复发率高达60%~80%。大多数的结石成分为混合性,其中草酸钙与磷酸钙的组合最常见。对4000颗含钙结石成分分析结果显示,70%的结石包含少量的磷酸钙。 Wu等通过单中心研究12846例患者的结石成分发现,2008—2012年磷酸钙结石发病率呈上升趋势。因此,越来越多的学者注意到磷酸钙在草酸钙晶体形成过程中可能起着重要的作用。研究结果显示,草酸钙结石的起源与肾钙化斑(即所谓的Randall斑)有着无法回避的联系,而钙化斑的成分却似乎是与草酸钙结石毫不相关的以羟磷灰石形式存在的磷酸钙。伴高钙尿症草酸钙结石患者肾内结晶形成的部位是髓襻细支的上皮基底膜表面,成分为磷灰石;而肠道旁路手术导致的高草酸尿症患者中,肾内初期形成的结晶成分同样属于磷灰石,但却存在于终末集合管的管腔内。由此可见,不同原因引起的肾内结晶起源部位不同,初期的结晶成分均为磷酸钙。提示磷酸钙可能在草酸钙晶体的形成中扮演着重要的角色。为了探讨出现这种现象的原因,需要阐明以下问题:①促使肾内结晶形成的原始动力是什么?②肾内结晶形成的调节因素是什么?③为什么促使草酸钙结石形成的肾钙化斑的成分是磷酸钙,而不是草酸钙或者其他成分?④为什么作为草酸钙结石起源的肾钙化斑位于肾小管的基底膜下而非管腔内?迄今为止,这些问题仍未得到合理的回答。多年来,我们围绕晶体-巨噬细胞反应在肾结石形成中的作用进行了系列研究:①在乙二醇诱导草酸钙肾结石的动物模型中,由于高草酸尿液的刺激,巨噬细胞能在骨桥蛋白的趋化作用下将结石晶体转运至大鼠肾脏的髓襻基底膜处。②通过光镜及电镜观察发现,巨噬细胞具有黏附、吞噬一水草酸钙晶体的能力;一水草酸钙晶体还可以刺激巨噬细胞还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶(nicotinamide adenine dinucleotide phosphate-oxidase,NADPH)的表达和活性升高,引起细胞氧化应激损伤。③高尿钙可导致草酸钙结石患者尿液中的单核细胞趋化蛋白-1(monocyte chemotactic protein 1,MCP-1)生成增多。
医院感染中产ESBLs大肠埃希菌基因型及同源性研究

大肠埃希菌是目前院内感染的主要病原菌之一,其与腹腔感染、尿路感染、伤口感染及血流感染等多种感染相关。由于广谱抗菌药物,尤其是三代头孢菌素的滥用及不合理使用,产超广谱β-内酰胺酶(ESBLs)大肠埃希菌检出率不断增高,给临床治疗带来很大困难。本研究同时分析采自我院患者和医院环境样本,采用多重PCR的方法对试验菌株ESBLs基因TEM、SHV、OXA、PER、VEB、GES、CTX及β-内酰胺酶基因AmpC进行扩增,确定基因型;脉冲场凝胶电泳技术(PFGE)对患者菌株和环境标本菌株进行同源性分析,以追踪传染源和传播途径。
丁二酸发酵新进展

丁二酸是一种重要的“C4平台化合物”,广泛应用于食品、医药、香料等工业,可取代苯合成250种以上的化工产品。微生物发酵法生产丁二酸因其环境友好、可利用废弃的生物质资源、能够固定温室气体CO_2等优点,成为近年来国内外的研究热点,美国能源部的报告将丁二酸列为未来12种最有潜力的生物基大宗化学品中的第一位。
Optimal Control of a Frictional Contact Problem

We consider a mathematical model which describes a contact between a deformable body and a foundation. The contact is bilateral and modelled with Tresca’s friction law. The goal of this paper is to study an optimal control problem which consists of leading the stress tensor as close as possible to a given target, by acting with a control on the boundary of the body. We state an optimal control problem which admits at least one solution. We also introduce the regularized control problem for which we study the convergence when the regularization parameter tends to zero. Finally, an optimally condition is established for this problem.
On Estimating Regression Coefficients in Seemingly Unrelated Regression System

In the system of m (m ≥ 2) seemingly unrelated regressions, we show that the Gauss-Markov estimator (GME) of any regression coefficients has unique simplified form, which exactly equals to the one-step covariance-adjusted estimator of the regression coefficients, and hence we conclude that for any finite k ≥ 2 the k-step covariance-adjusted estimator degenerates to the one-step covariance-adjusted estimator and the corresponding two-stage Aitken estimator has exactly one simplified form. Also, the unique simplified expression of the GME is just the estimator presented in the Theorem 1 of Wang’ work [1988]. A new estimate of regression coefficients in seemingly unrelated regression system, Science in China, Series A 10, 1033-1040].
银杏叶提取物对糖尿病患者血液流变学和微血管循环的影响

目的:观察银杏叶提取物对糖尿病患者血液流变学和微血管循环的影响,为银杏叶提取物治疗糖尿病提供一定的理论基础。方法:给予正常对照组和糖尿病患者舒血宁片(含银杏叶提取物9.6mg /片,口服治疗,每日3次,每次2片,8周后,均空腹抽血检测血液流变学各项指标和微血管形态。结果:治疗组空腹、餐后60min、餐后120min的血糖及胰岛素水平均有明显的下降;而对照组空腹、餐后60min、餐后120min的血糖及胰岛素水平均无明显变化,表明银杏叶提取物显著降低糖尿病患者血糖及胰岛素水平。对照组除血液流变学指标血小板聚集率从(86.29 ± 28.09) %降到了(33.80 ± 16.78) %,血沉从(45.18 ± 9.21) mm/L降到了(20.80 ± 9.04) mm/L(P < 0.01),其他血液流变学指标和微循环障碍有改善趋势,但无统计学意义。103例糖尿病患者的血液流变学指标如全血粘度从(14.03 ± 1.34) mPa·s降到了(7.98 ± 1.76) mPa·s、纤维蛋白原从(4.42 ± 0.71) g /L降低到了(3.21 ± 0.65) g /L,血小板聚集率从(87.92 ± 18.23) %降到了(39.08 ± 17.64) %,血沉从(48.33 ± 12.03) mm/L降到了(20.19 ± 7.19) mm/L(P < 0.01) 。微血管形态如毛细血管袢的数目、袢长、袢顶宽、及血管张力等项无明显差异,但治疗后血管袢的排列、变异程度、血管张力、变异率及血流速度等项趋于正常的患者明显增多。结论:银杏叶提取物对糖尿病患者的血液流变学和微循环障碍有明显的改善作用。
艾迪注射液联合FOLFOX4方案治疗结肠癌的临床研究

目的:探讨艾迪注射液联合FOLFOX4方案治疗对于结肠癌的临床价值。方法:收集2012年10月至2014年10月我院收治的96例晚期结肠癌患者的临床资料,按照随机数字表法均分为实验组和对照组,分别给予艾迪注射液联合FOLFOX4治疗和单纯FOLFOX4治疗,比较2组治疗效果。结果:实验组患者疗程结束后总有效率明显高于对照组;实验组患者治疗后WBC、Hb水平均明显高于对照组;实验组患者治疗后CD4~+、CD8~+、CD4~+ /CD8~+水平均明显高于对照组; 2组患者治疗后癌胚抗原(CEA)水平均明显下降,与同组治疗前比较,差异具有显著性;实验组患者治疗后CEA水平明显低于对照组;实验组患者Ⅰ ~Ⅱ度和Ⅲ ~ Ⅳ度WBC血液学毒性事件、感觉性神经病变、消化道反应发生率均明显低于对照组。结论:艾迪注射液能够有效提高化疗效果,缓解化疗对血象指标、免疫功能的影响,降低毒副反应发生率,临床效果显著。
六种中药四气药性对小鼠细胞因子受体的影响

目的:通过6种中药对小鼠的细胞因子受体影响探索四气药性的免疫分子机理。方法:SD小鼠随机分正常对照组、附子组、干姜组、肉桂组、黄连组、栀子组、知母组。6种中药的水浓缩煎剂每天分别对各自的给药组小鼠灌胃1次,连续10d,按照制定的实验动物中医证候体征级别计分评定观察小鼠情况,10d小鼠眼球取血,用Luminex xMAP technology液相悬浮芯片技术对小鼠血浆进行可溶性CD30(sCD30) 、可溶性白介素-1受体Ⅰ(sIL-1RⅠ) 、可溶性白介素-2受体α(sIL-2Rα) 、可溶性白介素-4受体(sIL- 4R) 、可溶性白介素-6受体(sIL-6R)等可溶性细胞因子受体指标的定量测定。结果:附子组的小鼠总进食量显著减少;肉桂组的小鼠总饮水量显著增加;各给药组小鼠胃粘膜出血溃疡显著增加;附子组的小鼠sCD30显著增加;肉桂组的小鼠sIL-1R1显著增加;附子组肉桂组的小鼠sIL-2Rα显著增加;黄连组的小鼠sIR-4R显著增加;附子组干姜组肉桂组黄连组的小鼠sIL-6R显著增加。结论:为受试中药药性的细胞因子受体信号转导表征免疫分子机理探索提供中医理论的实验基础。
Eco-environmental vulnerability assessment for large drinking water resource: a case study of Qiandao Lake Area, China

The Qiandao Lake Area (QLA) is of great significance in terms of drinking water supply in East Coast China as well as a nationally renowned tourist attraction. A series of laws and regulations regarding the QLA environment have been enacted and implemented throughout the past decade with the aim of negating the harmful effects associated with expanding urbanization and industrialization. In this research, an assessment framework was developed to analyze the eco-environ-mental vulnerability of the QLA from 1990-2010 by integrating fuzzy analytic hierarchy process (FAHP) and geographical information systems (GIS) in an attempt to gain insights into the status quo of the QLA so as to review and evaluate the effectiveness of the related policies. After processing and analyzing the temporal and spatial variation of eco-environmental vulnerability and major environmental issues in the QLA, we found that the state of eco-environmental vulnerability of the QLA was acceptable, though a moderate deterioration was detected during the study period. Furthermore, analysis of the combination of vulnerability and water quality indicated that the water quality showed signs of declination, though the overall status remained satisfactory. It was hence concluded that the collective protection and treatment actions were effective over the study period, whereas immediately stricter measures would be required for protecting the drinking water quality from domestic sewage and industrial wastewater. Finally, the spatial variation of the eco-environmental vulnerability assessment also implied that specifically more targeted measures should be adopted in respective regions for long-term sustainable development of the QLA.
Influence of pore pressure on tensile fracture growth in rocks: a new explanation based on numerical testing

The diffusion of pore fluid pressures may create both spatial and temporal effective stress gradients that influence or control the development and evolution of fractures within rock masses. To better understand the controls on fracturing behavior, numerical simulations are performed using a progressive fracture modeling approach that shares many of the same natural kinematic features in rocks, such as fracture growth, nucleation, and termination. First, the pinch-off breaking test is numerically performed to investigate the tensile failure of a rock specimen in a uniform pore pressure field. In this numerical simulation, both mechanical and hydrological properties of a suite of rocks are measured under simulated laboratory conditions. The complete tensional failure process of the rock specimen under pore pressure was reproduced. Second,a double-notched specimen is numerically extended to investigate how the water flow direction or pore pressure gradient influences the fracture growth. An exhaustive sensitivity study is conducted that examines the effects of varying both hydrological and mechanical boundary conditions. The simulation results indicate that local fluid pressure gradients strongly influence the state of stress in the solids and, thereby, fracture growth. Fracture and strength behavior is influenced not only by the pore pressure magnitude on a local scale around the fracture tip, but also by the orientation and distribution of pore pressure gradients on a global scale. Increasing the fracture growth rate increases the local model permeability and decreases the sample strength. The results of this study may provide useful information concerning the degree of hydrological and mechanical coupling action under geologic conditions.
A new method for recovering paleoporosity of sandstone: case study of middle Es3 member of Paleogene formation in Niuzhuang Sag,Dongying Depression,Bohai Bay Basin in China

This paper presents a new method for recovering paleoporosity of sandstone reservoirs and quantitatively defines the evolution process of porosity. This method is based on the principle that the present is the key to the past. We take the middle Es3 member in Niuzhuang Sag, Dongying Depression, and Bohai Bay Basin as an example. The method used in this study considers the present porosity as a constraint condition, and the influences of both constructive diagenesis and destructive diagenesis to divide the porosity evolution process into two independent processes, namely porosity increase and porosity decrease. An evolution model of sandstone porosity can be established by combining both the pore increase and pore decrease effects. Our study reveals that the porosity decrease model is a continuous function of burial depth and burial time, whereas the porosity increase model mainly occurs in an acidified window for paleo-temperature of 70°C to 90°C. The porosity evolution process can be divided into the following phases: normal compaction, acidification and pore increase, and post-acidification compaction. Thus, the porosity evolution model becomes a piecewise function of three subsections. Examples show that the method can be applied effectively in recovering the paleoporosity of sandstone reservoirs and simulating the porosity evolution process.
Amino acids 89-96 of Salmonella typhimurium flagellin represent the major domain responsible for TLR5-independent adjuvanticity in the humoral immune response

Toll-like receptor 5 (TLR5) signaling in response to flagellin is dispensable for inducing humoral immunity, but alterations of aa 89-96, the TLR5 binding site, significantly reduced the adjuvanticity of flagellin. These observations indicate that the underlying mechanism remains incompletely understood. Here, we found that the native form of Salmonella typhimurium aa 89-96-mutant flagellin extracted from flagella retains some TLR5 recognition activity, indicating that aa 89-96 is the primary, but not the only site that imparts TLR5 activity. Additionally, this mutation impaired the production of IL-1β and IL-18. Using TLR5KO mice, we found that aa 89-96 is critical for the humoral adjuvant effect, but this effect was independent of TLR5 activation triggered by this region of flagellin. In summary, our findings suggest that aa 89-96 of flagellin is not only the crucial site responsible for TLR5 recognition, but is also important for humoral immune adjuvanticity through a TLR5-independent pathway.
Argonaute 2 promotes angiogenesis via the PTEN/ VEGF signaling pathway in human hepatocellular carcinoma

Aim: Argonaute2 (AGO2) protein is the active part of RNA-induced silencing complex, cleaving the target mRNA strand complementary to their bound siRNA. An increasing number of miRNAs has been identified as essential to angiogenesis of hepatocellular carcinoma (HCC). In this study we investigated how AGO2 affected HCC angiogenesis. Methods: Human HCC cell lines HepG2, Hep3B,Huh7,SMMC-7721, Bel-7404, MHCC97-H and LM-3, and human umbilical vein endothelial cells (HUVEC) were tested. The expression of AGO2 in HCC cells was knocked down with siRNA and restored using recombinant adenovirus expressing Ago2. The levels of relevant mRNAs and proteins were examined using RT-PCR, Western blot and EILSA. Nude mice were implanted with Huh7 or SMMC-7721 cells, and tumor volumes were measured. After the mice were euthanized, the xenograft tumors were used for immunohistological analysis. Results: In 6 HCC cell lines, AGO2 protein expression was significantly correlated with VEGF expression (r=+0.79), and with VEGF secretion (r=+0.852). Knockdown of Ago2 in Huh7 cells and SMMC-7721 cells substantially decreased VEGF expression, whereas the restoration of AGO2 reversed both VEGF expression and secretion. Furthermore, knockdown of Ago2 significantly up-regulated the expression of PTEN (a tumor suppressor involved in the inhibition of HCC angiogenesis), and vice versa. Moreover, the specific PTEN inhibitor bisperoxovanadate (7,14, 28 nmol/L) dose-dependently restored the expression of VEGF and the capacity of HCC cells to induce HUVECs to form capillary tubule structures. In the xenograft nude mice,knockdown of Ago2 markedly suppressed the tumor growth and decreased PTEN expression and CD31-positive microvascular in the xenograft tumors. Conclusion: A direct relationship exists between the miRNA processing machinery AGO2 and HCC angiogenesis that is mediated by the AGO2/PTEN/VEGF signaling pathway. The results suggest the high value of Ago2 knockdown in anti-angiogenesis therapy for HCC.
甲状腺相关性眼病严重程度影响因素分析

甲状腺相关性眼病(thyroid associated ophthalmopathy, TAO)是器官特异性自身免疫性疾病,其发病的影响因素众多,如性别、家族史、吸烟、促甲状腺激素(thyroid stimulating hormone, TSH)水平、~(131)I治疗、甲状腺炎症病史、外伤、手术、功能异常、感染、辐射、恶性肿瘤等。但有关TAO严重程度的相关因素的研究较少,结论不一。现将我院收治的TAO患者的严重程度及其影响因素报道如下。
热浸镀Zn - Al - Mg - Ni - V合金镀层的组织及耐蚀性

为了提高镀锌层的耐蚀性,在纯锌浴中加入质量分数小于0.5%的Ni,V,Al,Mg元素,制备了热浸Zn-Al-Mg-Ni-V合金镀层。利用扫描电镜(SEM)、能谱分析对镀层组织、结构成分进行观察和分析,通过中性盐雾试验和电化学试验将热浸Zn-Al-Mg-Ni-V合金镀层与纯锌镀层的耐蚀性进行对比。结果表明:热浸Zn-Al-Mg-Ni-V合金镀层的耐蚀性较传统镀锌层的有了很大提高,镀层主体结构没有发生明显变化。
水性聚氨酯/聚苯胺导电防腐蚀涂料的研制

为了解决传统水性聚氨酯涂料导电性不佳的问题,通过将具有导电功能的防腐蚀材料聚苯胺加入到聚氨酯成膜物中,制成了导电性好、防腐蚀性能优良的新型防腐蚀涂料,并考察了其综合性能。结果表明:聚氨酯与聚苯胺比例在14: 1左右时漆膜性能最好;温度对其干燥时间影响较小,漆膜在30 ℃及以上10 min内即可实干;漆膜的耐水性良好。漆膜性能测试结果完全符合国家标准,表明该涂料具有优良的防腐蚀性能。
海底管道阴极保护电位的分布

海底管道将穿越不同环境的海水,海水的电导率和管道本身防腐蚀涂层的完好率,将极大地影响海底管道阴极保护电位的分布,而实测又受到多种因素的限制。为了准确地获取其真实分布状态,建立了海底管道阴极保护电位分布的数学模型,采用FLUENT软件对海底管道阴极保护电位进行模拟计算,研究了不同海水电导率和管道涂层破损率对管道阴极保护电位分布的影响。结果表明:模拟计算结果与实际测量结果吻合良好;阴极保护电位随海水电导率的升高而降低;管道涂层的破损率越低,越有利于阴极保护电位的均匀分布。