mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Observations of Freak Waves in Random Wave Field in 2D Experimental Wave Flume?

Long time series of wave field are experimentally simulated by JONSWAP spectra with random phases in a 2D wave flume. Statistic properties of wave surface, such as significant wave height, skewness and kurtosis, are analyzed, and the freak wave occurrence probability and its relations with Benjamin-Feir index (BFI) are also investigated. The results show that the skewness and the kurtosis are significantly dependent on the wave steepness, and the kurtosis increases along the flume when BFI is large. The freak waves are observed in random wave groups. They occur more frequently than expected, especially for the wave groups with large BFI.
影响美托洛尔治疗效果的药物基因组学研究进展

药物临床反应个体差异与药物基因组学关系密切。研究证明,药物代谢酶、药物受体及其下游蛋白基因的遗传变异是造成药物反应个体差异的主要因素。美托洛尔作为治疗高血压及心血管疾病的经典药物被广泛应用于临床,但其安全范围窄,较易发生药物不良反应。近年研究发现,药物代谢酶CYP2D6、β1肾上腺受体蛋白以及G蛋白基因的遗传多态性是决定美托洛尔疗效个体差异的主要内在因素。本文作者对导致美托洛尔药物反应个体差异的相关基因的多态性及其对美托洛尔疗效的影响进行评述,以期为减少美托洛尔不良反应及提高临床疗效提供参考。
术前诊断残角子宫妊娠1例报告

残角子宫系一侧副中肾管发育,另一侧副中肾管中下段发育缺陷,形成残角子宫。输卵管及卵巢正常,同时多伴有同侧泌尿器官发育畸形。分为3种类型:Ⅰ型残角子宫有宫腔,并与单角子宫腔相通;Ⅱ型残角子宫有宫腔,并与单角子宫腔不相通;Ⅲ型为实体残角子宫,仅以纤维带相连单角子宫。残角子宫妊娠是指受精卵经残角子宫侧输卵管进人残角子宫内着床和发育的一种异位妊娠。因临床罕见,早期症状和体征不典型,与一般异位妊娠有相似之处,但停经时间较长,多超过3个月。临床上误诊率高,因残角子宫肌壁发育不良,不能承受较大的胎儿,多于妊娠中晚期发生破裂,或者因子宫肌壁发育不良,底蜕膜发育不良,为获取更多血供,胎盘组织从底蜕膜发育不全处深人子宫肌层,甚至达到浆膜层,损伤子宫表面血管,导致腹腔内出血和植人性胎盘。残角子宫妊娠一旦发生破裂可致严重的失血性休克,危及患者生命。近年来,随着超声诊断技术的进展,一些残角子宫妊娠得以早期诊断和治疗,如本例患者术前根据超声检查可基本确诊。但绝大多数还是在手术中得以证实。妊娠至足月极为罕见,截止到1995年,世界上仅有6例残角子宫妊娠足月活胎的报道。残角子宫妊娠一经确诊应行残角子宫及其同侧输卵管切除术。术中应同时切除患侧输卵管,以免再次发生输卵管妊娠。
Effectiveness of sloping agricultural land technology on soil fertility status of mid-hills in Nepal

Hedgerows with intercropping systems were established at the ICIMOD test and demonstration site at Godawari to assess the effective-ness of Sloping Agricultural Land Technology (SALT) in reducing run-off water volume, controlling soil loss, increasing crop production, and improving soil fertility in the mid-hills of Nepal. Runoff water volume (1996-2002), soil loss (1996-2002) and maize yield (1995-2001), and soil fertility-related parameters were assessed on SALT models with three factors:the type of nitrogen-fixing plant, the farmers’ practice, and fertilizer use. Results showed a significant effect of Alnus nepalensis and/or Indigofera dosua on runoff water volume, soil loss, crop produc-tion, soil water retention, and soil nutrients (NPK). Farmers’ practice and fertilization did not play a significant role in reducing runoff water and soil loss. However, farmers’ practice significantly increased crop produc-tion. Therefore, integrating soil conservation approaches on SALT sys-tems enhances stable economic output to hills and mountain farmers.
Effect of seed treatment for laboratory germination of Albizia chinensis

Seeds of Albizia chinensis(Osb.) Merr. in addition to water were also treated with different treatments by incubating in ethyl alcohol, acetone, and petroleum ether at room temperature for different durations. Seed heat treatment was done at temperatures of 30, 40 and 60°C for different durations up to 24 h. To overcome dormancy caused by the impermeable seed coat, seeds were nicked and also treated with concen-trated sulphuric acid for different durations. Seeds responded to treat-ments with sulphuric acid and nicking only. Treatment with sulphuric acid for 20 and 30 min showed maximum germination at all incubation temperatures as compared to untreated controls and seeds treated with sulphuric acid for 10 min and nicking. Seedling length was greatest from seeds treated with sulphuric acid for 20 and 30 min and incubated at 30 °C. Seedling dry weight was highest from nicked seeds incubated at 20°C. The most favourable incubation temperature was 30 °C as evidenced from GRI (germination rate index) and GV (germination value). After ascertaining the seed response and performance we recommend that seeds of Albizia chinensis be treated with sulphuric acid for 20 or 30 min and incubation temperature of 25 to 30°C.
Do vegetative and reproductive phenophases of deciduous tropical spe-cies respond similarly to rainfall pulses?

Deciduous trees with high-density wood that occur in dry seasonal tropical regions respond to rainfall seasonality with synchrony in phenophases. However, they may exhibit interannual differences in synchrony and intensity of phenophases, as strategy for large variations in duration and intensity of rainfall pulses. Nevertheless, it remains unknown how phenophases of deciduous trees of the Brazilian semi-arid region respond to rainfall variations. The phenology of Cordia oncocalyx was monitored in deciduous thorny woodland (Caatinga), from April 2009 to March 2011, and was correlated with rainfall, soil humidity, temperature, and photoperiod. The rainy years 2009 and 2011 exhibited higher duration of rainfall pulses and lower frequency of interpulses, but in 2010 pulse duration and total rainfall were lower. Circular statistics showed leaf flush followed by flowering and fruiting in the rainy season, and leaf fall and seed dispersal in the dry season. Both the vegetative and reproductive phenophases respond similarly to variations in rain pulses, with adjustments in time, duration, and intensity, which were correlated with variations in rainfall and soil humidity, excluding photoperiod as a trigger. Total defoliation occurred in the driest months of each year, November to December 2009 and August to October 2010. A sporadic leaf flush in November 2010 was triggered by occasional rains. Vegeta-tive and reproductive synchronies were high in rainy years, but in 2010 synchrony was low, flowering was delayed and reduced, and, despite the low fruiting, we recorded high density in the seed bank. Lower syn-chrony, temporal separation of phenophases, and storage of fruits on the ground were risk-spreading strategies used by the population in the dry year, which suggest intrapopulation variability in responses to hydric stress. We believe that this plasticity contributes to high species density in the Caatinga.
Effect of rhizobial inoculations on nitrogen metabolism of Albizia lebbek seedlings

Rhizobia were isolated from Albizia lebbek (L) Benth. seed-lings collected from six different places, tested against the nodulation test and inoculated into 45 day old Albizia lebbek seedlings in sterile soil mixture under glass house conditions. After a period of two and three months, the plant samples were taken to study the influence of inoculation treatments on nitrogen fixation, assimilation, biomass production and nitrogen content in different plant parts. The seedlings inoculated with isolates III(1) and III(2) of Lalpani had the maximum nodular biomass, specific (13.73 and 13.59 μ mole C_2H_2 reduced h~(-1), respectively) and total nitrogenase (11.80 and 11.16 μ mole C_2H_2 reduced h~(-1), respectively) activities in their nodules statistically at par with each other. These also exhibited high nitrate reductase activity in different plant parts. The seedlings inoculated with slow growing isolates viz. I(2) and IV(1) and the control were amongst poor performers for biomass production, nitro-genase activity and nitrate reductase activity in different plant parts. The minimum nitrogenase (specific and total) activities and low nitrogen content (%) in leaves, stems and roots were estimated in seedlings in-oculated with isolate II(4) of Barkot. Nodular biomass was recorded as an indicator of nitrogen fixation activity rather than the number of nodules per plant. The isolates III(1) and III(2) can be utilized to enhance produc-tivity in afforestation and reforestation programmes.
Mycorrhizal synthesis between Pisolithus arhizus and adult clones of Arbutus unedo in vitro and in nursery

Arbutoid mycorrhizae were synthesized between adult se-lected clones of Arbutus unedo L. and Pisolithus arhizus. Two micro-propagated clones were tested:AL1, in vitro and C1 (acclimatized plants) in nursery and later in a field trial. In vitro, rooted shoots were trans-ferred to test tubes containing the substrate previously inoculated with mycelium cultured on agar. In the nursery, two inoculation treatments were tested (vegetative inocula or dry sporocarps) and compared to con-trol plants. In the field trial, plants from nursery inoculation treatments were compared and an additional control treatment using seedlings was implemented. Plant height was evaluated 4 months later in the nursery and 20 months later in the field trial. Roots were examined by morpho-logical and histological studies: a) in vitro plantlets one month after inoculation and nine months after acclimatization;and b) 20 months after the field trial was established. Arbutoid mycorrhizae were observed in vitro one month after inoculation, indicating compatibility between A. unedo and P. arhizus. These showed the presence of a mantle, Hartig net, and intracellular hyphal complexes confined to the epidermal root cells. Arbutoid mycorrhizae were also observed nine months after acclimatiza-tion in inoculated and control plants. In order to confirm the identity of mycorrhizae, molecular techniques were used, in previously inoculated in vitro plants, 12 months after acclimatization. Thelephora and Hebeloma mycorrhizae, two types of highly competitive and widespread my-corrhizae on nurseries were identified. In the nursery, dry sporocarp treatment improved plant height after four months. In a field trial (20 months later), plants growth did not show significant differences. By this time, mycorrhized roots with Cenococcum geophilum and other types were identified. These results and their implications on A. unedo breed-ing program are discussed.
Hybridization and genome evolution I: The role of contingency during hybrid speciation

Homoploid hybrid speciation (HHS) involves the recombination of two differentiated genomes into a novel, functional one without a change in chromosome number. Theoretically, there are numerous ways for two parental genomes to recombine. Hence, chance may play a large role in the formation of a hybrid species. If these genome combinations can evolve rapidly following hybridization and sympatric situations are numerous, recurrent homoploid hybrid speciation is a possibility. We argue that three different, but not mutually exclusive, types of contingencies could influence this process. First, many of these “hopeful monsters” of recombinant parent genotypes would likely have low fitness. Only specific combinations of parental genomic contributions may produce viable, intra-fertile hybrid species able to accommodate potential constraints arising from intragenomic conflict. Second, ecological conditions (competition, geography of the contact zones or the initial frequency of both parent species) might favor different outcomes ranging from sympatric coexistence to the formation of hybrid swarms and ultimately hybrid speciation. Finally, history may also play an important role in promoting or constraining recurrent HHS if multiple hybridization events occur sequentially and parental divergence or isolation differs along this continuum. We discuss under which conditions HHS may occur multiple times in parallel and to what extent recombination and selection may fuse the parent genomes in the same or different ways. We conclude by examining different approaches that might help to solve this intriguing evolutionary puzzle.
High-Sensitivity Acquisition of Ultrahigh Dynamic Direct Sequence Spread Spectrum Signals in Space Communications

According to the requirements of the high-sensitivity acquisition of Direct Sequence Spread Spectrum (DSSS) signals under ultrahigh dynamic environments in space communications, a three-dimensional joint search of the phase of Pseudo-Noise-code (PN-code), Doppler frequency and its rate-of-change is presented to achieve high sensitivity in sensing high-frequency dynamics. By eliminating the correlation peak loss caused by ultrahigh Doppler frequency and its rate-of-change offset, the proposed method improves the acquisition sensitivity by increasing the non-coherent accumulation time. The validity of the algorithm is proved by theoretical analysis and simulation results. It is shown that signals with a carrier- to-noise ratio as low as 39 dBHz can be captured with high performance when the Doppler frequency is up to ±1 MHz and its rate-of-change is up to ±200 kHz/s.
Cross-Layer Design and Ant-Colony Optimization Based Routing Algorithm for Low Earth Orbit Satellite Networks

To improve the robustness of the Low Earth Orbit (LEO) satellites networks and realise load balancing, a Cross-layer design and Antcolony optimization based Loadbal-ancing routing algorithm for LEO Satellite Networks (CAL-LSN) is proposed in this paper. In CAL- LSN, mobile agents are used to gather routing information actively. CAL-LSN can utilise the information of the physical layer to make routing decision during the route construction phase. In order to achieve load balancing, CAL- LSN makes use of a multi-objective optimization model. Meanwhile, how to take the value of some key parameters is discussed while designing the algorithm so as to improve the reliability. The performance is measured by the packet delivery rate, the end-to-end delay, the link utilization and delay jitter. Simulation results show that CAL-LSN performs well in bal-ancing traffic load and increasing the packet delivery rate. Meanwhile, the end-to-end delay and delay jitter performance can meet the requirement of video transmission.
Sodium tanshinone IIA sulfonate protects rat myocardium against ischemia-reperfusion injury via activation of PI3K/Akt/FOXO3A/Bim pathway

Aim To investigate the mechanisms underlying the protective effects of sodium tanshinone IIA sulfonate (STS) in an ischemia-reperfusion (I/R)-induced rat myocardial injury model. Methods: Male SD rats were iv injected with STS, STS+LY294002, or saline (NS) for 15 d. Then the hearts were subjected to 30 min of global ischemia followed by 2 h of reperfusion. Cardiac function, infarction size and area at risk were assessed. Cell apoptosis was evaluated with TUNEL staining, DNA laddering and measuring caspase-3 activity. In addition, isolated cardiomyocytes of neonatal rats were pretreated with the above drugs, then exposed to H2O2 (200 μmol/L) for 1 h. Cell apoptosis was detected using flow cytometric assay. The levels of p-Akt, p-FOXO3A and Bim were examined with immunoblotting. Results: Compared to NS group, administration of STS (20 mg/kg) significantly reduced myocardial infarct size (40.28%±5.36% in STS group vs 59.52%±7.28% in NS group), and improved the myocardial function as demonstrated by the increased values of dp/dtmax, LVDP and coronary flow at different reperfusion time stages. Furthermore, STS significantly decreased the rate of apoptotic cells (15.11%±3.71% in STS group vs 38.21%±7.83% in NS group), and reduced caspase-3 activity to nearly a quarter of that in NS group. Moreover, STS significantly increased the phosphorylation of Akt and its downstream target FOXO3A, and decreased the expression of pro-apoptotic gene Bim. Co-treatment with the PI3K inhibitor LY294002 (40 mg/kg) partially countered the protective effects induced by STS treatment. In isolated cardiomyocytes, STS exerted similar protective effects as shown in the ex vivo I/R model. Conclusion: STS pretreatment reduces infarct size and improves cardiac function in an I/R-induced rat myocardial injury model via activation of Akt/FOXO3A/Bim-mediated signal pathway.
Essential role of autophagy in fucoxanthin-induced cytotoxicity to human epithelial cervical cancer HeLa cells

Aim To investigate the effects and the molecular mechanisms of fucoxanthin, a major carotenoid found in edible seaweed, on HeLa cells. Methods: The cytotoxicity of fucoxanthin was evaluated using MTT assay. Cell cycle and apoptosis were evaluated using flow cytometric analysis. Autophagy was detected with acridine orange staining and transient transfection of the GFP-LC3 plasmid into the cells. Protein expression was detected with Western blotting. Results: Treatment of HeLa cells with fucoxanthin (10–80 μmol/L) for 48 h caused dose-dependent cytotoxicity with an IC50 value of 55.1±7.6 μmol/L. Fucoxanthin (10, 20, and 40 μmol/L) dose-dependently induced G0/G1 arrest, but did not change the apoptosis of HeLa cells. The same concentrations of fucoxanthin dose-dependently increased the protein expression of LC3 II (the autophagosome marker) and Beclin 1 (the initiation factor for autophagosome formation) in HeLa cells. Moreover, fucoxanthin dose-dependently decreased the levels of phosphorylated Akt and its downstream proteins p53, p70S6K, and mTOR, and increases the expression of PTEN in HeLa cells. Pretreatment of HeLa cells with 3-methyladenine (5 mmol/L) blocked the cytotoxic effect of fucoxanthin as well as fucoxanthin-induced autophagy. Conclusion: Fucoxanthin exerts autophagy-dependent cytotoxic effect in HeLa cells via inhibition of Akt/mTOR signaling pathway.
失眠的中成药治疗

失眠是临床常见的疾病和症状,多需要长期服药治疗,而中成药在其治疗上具有一定的优势。本文根据作者的经验,论述了中成药在治疗失眠中的应用情况,提出临床应用要注意把握病机、辨证使用中成药;可以取长补短、联合使用中成药,另外还要熟悉药物的药性,以避免副作用的产生。
锁定钢板治疗股骨远端骨折失误7例分析

股骨远端骨折的理想内固定尚未明确,在锁定钢板问世以前,其常用的固定方法为95°角钢板、动力髁螺钉、解剖型外侧支撑钢板及逆行交锁髓内钉等。锁定钢板的出现为临床上治疗严重粉碎性骨折、骨质疏松性骨折、关节周围骨折等棘手的骨折提供了新的选择,越来越多的医生使用锁定加压钢板(locking compression plates,LCP)治疗股骨远端骨折,取得较好的疗效,但由于对锁定钢板的基本概念不了解,没有正确按照其原理使用,又在锁定钢板的运用中出现了新的问题,导致其失效或断裂。本组7例均是在使用LCP治疗股骨远端骨折中由于使用不当而导致内固定失效,现报道如下,供同道参考。
晚期胃癌患者行旷置胃空肠吻合术对生存质量的影响

目的:探讨旷置胃空肠吻合术对晚期胃癌患者生存质量的影响,并与Braun法胃肠吻合手术的治疗效果进行对比。方法:选取2009年1月-2012年1月收治的62例需接受手术治疗的晚期胃癌患者为研究对象,根据患者治疗意愿将其分为旷置胃空肠吻合手术组32例(观察组)以及Braun法胃肠吻合手术组30例(对照组),术后观察两组患者临床疗效,并对患者随访1.5年,观察两组患者病死率及平均生存时间及对患者进行QOL生活质量评分,对比分析两种治疗对患者生存质量的影响。结果:观察组术中出血量、手术时间、平均住院时间与对照组比较,无统计学意义(P>0.05);观察组术后并发症发生率、病死率较低,平均生存时间较长,两组间差异具有统计学意义(P<0.05)。术后3个月对患者进行QOL生活质量评分,观察组食欲、精神、睡眠、疼痛、疲乏、自身对癌症认识、治疗态度、日常生活以及总评分均优于治疗前及对照组,差异有统计学意义(P<0.05)。结论:对晚期胃癌患者应用旷置胃空肠吻合手术进行治疗,能有效降低患者术后并发症及病死率,提高患者术后生存质量。
中药药理性预适应对心肌缺血再灌损伤的保护作用

1986年,Murry 等在犬缺血再灌模型实验中观察到预先一次或反复短时间缺血可以对心肌缺血/再灌注损伤有保护作用,认为主要是由于心肌有较好的耐受能力,可以延缓心肌细胞死亡,并首次提出了心肌缺血预适应(IPC)。目前,IPC 已越来越被研究人员和临床工作者所重视。
中药治疗病毒性心肌炎的实验研究进展

病毒性心肌炎(vital myocarditis, VMC)是临床最常见的感染性心肌病,患病者主要为青少年和成人, 8% ~12%会出现粹死现象。柯萨奇病毒主要包括疱疹病毒、肠道病毒、艾巴氏病毒、腺病毒等。柯萨奇病毒 Β 组病毒(Coxsackievirus Β, CVB)是 VMC最常见致病原,约占30%?50%。中医学中尚无特定的病名与本病相对应,多属“温毒”、“心悸”、“怔忡”、 “胸搏”范畴,此外还与汗证、虚劳、猝死等相关。目前西医主要采用抗病毒、抗氧化、改善心肌代谢、调节免疫等方法治疗,无针对性治疗药物,而中医学对本病标本兼治取得了较好疗效,在临床上应用较为广泛。本文就近年来的一些研究进行了总结,现概述如下。
头胸腹联体双胎1例报道及文献回顾

讨论联体双胎指两个胎儿的某些部位联结在一起,为受精第13天后单卵单羊膜腔双胎受精卵分裂的两个胚胎上的生发中心未能完全分开,两个胚胎共享中间区域,导致身体部分融合而形成联体畸形,在新生儿中发生率为1/80 000?1/50 000。联体双胎的联结部位和程度各有不同,形式多样,可分为对称性及非对称性联胎。非对称性联胎指相连两个胎儿大小发育极不相称,较大者发育尚正常,为主胎,较小者发育不全,附着于主胎的某一部分,为寄生胎。根据其所在位置,寄生胎又可分为体外寄生胎与体内寄生胎。本例即属体外寄生胎。
城市水环境系统综合评价体系研究与示范

以构建科学性和可操作性强的城市水环境系统综合评价体系为目标,结合我国城市水环境系统特征与现行城市水环境相关评价办法,开展了城市水环境系统相关评价指标体系调研与比较、城市水环境系统构成要素基础信息数据库构建、城市水环境系统综合评价指标体系确定、城市水环境系统综合评价网络管理平台开发、综合评价网络管理平台全过程质量控制等系统研究,突破了城市水环境系统构成要素基础指标筛选、城市水环境系统基础指标交互影响机制、城市水环境系统综合评价指标体系及评价模型构建、网络管理平台实现等关键技术,并在不同行政级别的城市、同一个省内的城市、流域城市群3种情形进行了示范应用与验证。城市水环境系统综合评价体系与网络管理平台的不断完善和推广应用,将为我国城市、城市群和流域水环境综合治理工作评价与考核提供科学依据。
福州市暴雨强度公式优化研究

暴雨强度是城市雨水工程规划设计的重要参数,其计算结果直接影响城市雨水工程的安全性与经济性。通过分析目前福州市暴雨强度公式使用中遇到的问题,确定优化的重点,基于福州市新旧城区雨量站1971~2011年的降雨资料,按年最大值选样,延长降雨历时并扩大重现期,采用P- III分布进行分布曲线拟合,分别采用优选回归法、高斯一牛顿法和麦夸尔特法求解暴雨强度公式的4个参数,根据误差最小原则,确定最优求参方法,比较“优化公式”与“现行公式”的暴雨强度,进一步分析城市化开发程度对暴雨强度的影响。研究结果表明:优化后的暴雨强度公式精度满足国家规范的相关要求;长历时降雨选择“优化公式”计算暴雨强度更为安全;城市化建设对短历时设计暴雨强度影响很大,不同开发强度城区的暴雨强度存在一定差异.
Stability analysis of the fluttering and autorotation of flow-induced rotation of a hinged flat plate

This work describes investigations performed on the interaction of uniform current and freely rotating plate about a fixed vertical axis. Fluttering and autorotation are two different motions that may occur during the flow induced rotation. The dimensional analysis proves that the motion in flow induced rotation motion is governed essentially by the dimensionless moment of inertia and Reynolds number. Certain combinations define the stability boundaries between fluttering and autorotation. Fluttering is oscillation of body about a vertical axis and the autorotation is a name given to the case when the body turns continuously about the vertical axis. First, the loads and moment coefficients are calculated by experiments and streamline theory for different angles of attack for a fixed flat plate. Then for dynamic case, a bifurcation diagram is presented based on experiments to classify different motion states of flow induced rotation. Finally, a dynamical model is proposed for stability analysis of flow induced rotation of a flat plate.
藏药八味獐牙菜丸中芒果苷的含量测定

目的:建立测定藏药八味獐牙菜丸中芒果苷含量的方法。方法:采用反相高效液相色谱法进行测定,Waters SunFire C_(18)柱;流动相为甲醇-0.1%冰醋酸(21: 79);流速1.0 mL·min~(-1);检测波长260 nm。外标法定量其中芒果苷的含量。结果:芒果苷在0.0162~0.5400 μg范围内与峰面积呈良好的线性关系(r=0.999 7),平均回收率为99.15%,RSD为1.88%。结论:所建立的方法简便、准确,具有良好地重现性和准确率,可作为八味獐牙菜丸中芒果苷成分的测定。
闭合性十二指肠损伤23例临床分析

十二指肠损伤属严重脏器伤,病死率高,对外科医生是一大挑战。十二指肠解剖位置深在,且无特异性临床症状,术前诊断十分困难,一旦延误诊断,从十二指肠流出的液体可达6 L/d,并形成篓道,大量有活性的酶释放到腹膜后和腹腔会造成致命的后果。现将我院近10年收治的十二指肠损伤患者23例进行回顾性分析,报告如下。
ARHI和突变型P53在人脑星形胶质细胞瘤中的表达及与恶性程度的相关性

目的初探ARHI和突变型P53在人脑星形胶质细胞瘤中的表达及与恶性程度的相关性。方法采用免疫组织化学S-P法检测10例正常脑组织、30例低级别胶质瘤(Ⅰ、Ⅱ级)和39例高级别胶质瘤(Ⅲ、Ⅳ级)ARHI和突变型P53的表达情况,并分析二者之间的关系。结果与正常脑组织相比,ARHI在人脑胶质瘤中的表达显著降低,突变型P53在人脑胶质瘤中的表达显著增加。通过阳性区平均积分光密度定量分析,结果显示,正常组、低级别胶质瘤组和高级别胶质瘤组ARHI和突变型P53分别为(176.41±5.37)和(104.52±4.57)、(148.73±3.82)和(126.68±2.46)、(123.59±3.74)和(147.23±4.97),各组之间ARHI和突变型P53的测定值有统计学差异(P<0.01),并且二者在人脑胶质瘤中的表达呈负相关(r=-0.532,P<0.05)。结论ARHI和突变型P53可能成为判断星形胶质细胞瘤恶性程度、侵袭性及预后的有效指标。
Isolated extramedullary ocular relapse of acute lymphoblastic leukemia after peripheral blood stem cell transplantation

Isolated extramedullary ocular relapse of acute lymphoblastic leukemia (ALL) after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) without concomitant involvement of the bone marrow is very rare,while the common sites of extramedullary relapse are the central nervous system, skin, bone, and breasts.1 This is the report of isolated ocular relapse without any extra-ocular involvement of ALL after allo-PBSCT confirmed by histopathology.
射频组织焊接技术用于结直肠吻合的探索性研究

利用射频能量焊接肠组织,研究压合压强、功率、温度参数对焊接效果的影响。利用自制的焊接器械和射频能量平台,进行焊接猪离体结直肠组织实验。实验参数为:射频频率450 kHz,输出功率恒定(50和70 W),在压合压强(1 125和2 658 kPa)下持续焊接肠组织240 s,控制焊接过程中的最高温度为(95±5) ℃。实验完成后通过组织病理切片对比不同条件下肠道焊接的牢固程度、热损伤情况。结果表明:在50和70 W功率下,压合压强1 125 kPa的组,焊接部位浆膜层紧紧贴合,周围组织结构保持完好;压合压强2 658 kPa的组,焊接部位浆膜层未完全融合,且周围黏膜损伤较明显。最高焊接温度控制在(95±5)℃时,功率50和70 W的组焊接效果在组织病理切片观察中无明显差异。射频能量焊接结直肠可行性良好,但压合压强过大易导致焊接部位周围组织损伤;且考虑到焊接部位周围的温度分布不均匀,不可单一用温度参数作为判断焊接效果的标准。
Management of postoperative instrumented spinal wound infection

Background Wound infection following spinal instrumented surgery is a frequent complication. The optimal treatment of acute deep wound infection following spinal instrumentation fusion remains controversial because of variability in cohort identification, definition of an infection, and the instrument used to measure outcomes. This retrospective study evaluated the clinical curative effect for postoperative spinal infection after instrumented spine fusion with extensive debridement, or implant removal. Methods From January 2004 to October 2009, 851 patients were identified who underwent surgical treatment of spinal diseases. The medical records of patients who developed infections were reviewed in detail. Results Of 851 patients, 41 (4.9%) developed an infection. Thirty-three were acute, and eight were delayed. Acute infected cases were managed with antibiotic therapy, and aggressive debridement of the wound and soft tissues leaving all instrumentation in situ in all but one patient. The most common symptoms of acute infection included: posterior incisional drainage (26 of 33 patients), back pain (22 of 33 patients) and fever (13 of 33 patients). Among patients with delayed onset infection, five of eight patients had local pain, four of eight patients had incision drainage, and one patient had a prolonged period of intermittent fever. The most frequent causative organism for postoperative spinal infection following spine surgery is Staphylococcus aureus. Pseudarthrosis was noted in long-term follow-up in four of 41 patients. Conclusions We recommend irrigation and debridement, no instrumentation removal, and, if necessary, repeat debridement followed by delayed primary closure for the treatment of acute deep infection with instrumentation.