mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
姜薯的组织培养与快速繁殖

1 植物名称 姜薯(Dioscorea alata L.).2 材料类别 块根.3 培养条件 以MS为基本培养基.(1)诱导培养基:MS+2,4-D 2.0 mg·L~(-1)(单位下同)+6-BA 0.5;(2)芽分化培养基:MS+6-BA 3.0+NAA 0.5;(3)生根培养基:1/2MS+NAA 0.5+IBA 0.5.以上培养基均附加30 g·L~(-1)蔗糖和7 g·L~(-1)琼脂,pH 5.8.培养温度为为(26～2)℃,光照时间为12 h.d~(-1),光照强度为30～40μmol·m~(-2)·s~(-1).
孕妇尿液人巨细胞病毒DNA筛查及血液HCMV抗体测定的对照研究

目的 对于孕妇尿液进行HCMV-DNA筛查,减少和有效地避免胎儿及新生儿的HCMV感染.HCMV-DNA筛查同时进行HCMV抗体检测,明确诊断HCMV感染的灵敏、准确方法.方法 应用荧光定量PCR(FQ-PCR)方法进行尿液HCMV-DNA测定.血液HCMV IgM、IgG抗体检测应用酶联免疫(ELISA)方法.结果 筛查6568例孕妇尿HCMV-DNA,阳性273例,阳性率为4.2%.孕妇在12-20周者,阳性率为10.3%;孕妇在21-30周者,阳性率为33.3%;孕妇在3l～39周者,阳性率为56.4%.在273例尿液HCMV-DNA阳性者中,56例同时进行血液HCMV IgM、IgG抗体检测,IgM抗体阳性者2例,IgG抗体阳性者22例.结论 在孕妇HCMV感染的筛查与诊断中FQ-PCR是灵敏准确的方法.孕妇中HCMV-DNA筛查是保证母婴健康,提高人口素质的保障.
金融危机下的农村发展与农民增收问题——"湖湘三农论坛"2009年·常德会议综述

农民是中国最大的社会群体,"三农"问题的核心是增加农民收入.改革开放以来,特别是近几年来,随着中国政府各项惠农政策的逐步实施,"三农"问题的严峻程度有了一定的缓和.
农村消费者生态心理意识对生态消费影响的实证分析

本文通过问卷调查,运用描述性统计方法、K-Means 聚类方法和Logistic回归模型,就农村消费者生态心理意识对生态消费的影响进行了实证研究.结果表明:农村消费者的环境关心程度、污染感知程度显著正向影响其生态消费,对乱丢垃圾问题的态度正向影响其生态消费但不显著.就人口统计特征而言,年龄显著正向影响其生态消费,文化水平显著负向影响其生态消费,特定农村消费者群体的生态心理意识强度存在显著差异.
Assessment of genetic diversity in broomcorn millet (Panicum miliaceum L.) using SSR markers

The genetic diversity of 118 accessions of broomcorn millet (Panicum miliaceum L.), collected from various ecological areas, was analyzed. Using 46 SSR (Simple Sequence Repeat) polymorphic markers from rice, wheat, oat and barley, a total of 226 alleles were found, which exhibited moderate level of diversity. The number of alleles per primer ranged from two to nine, with an average of 4.91.The range of polymorphism information content (PIC) was 0.284-0.980 (average, 0.793). The expected heterozygosity (He) varied frorm 0.346 to 0.989, with an average of 0.834. The average coefficient of the genetic similarity of SSR markers among the 118 accessions was 0.609, and it ranged from 0.461 to 0.851. The UPGMA (Unweight Pair Group Method with Arithmetic Mean) clustering analysis at the genetic similarity value of 0.609 grouped the 118 accessions into five groups. Mantel test meant that geographical origin and genetic distance presented positive correlation. The clustering results were consistent with known information on ecological growing areas. The genetic similarity coefficient of the accessions in the Loess Plateau ecotype was significantly lower than those in the other ecotypes. It indicates that the highest level of genetic diversity occurred in the Loess Plateau, which is probably the original site of Panicum miliaceum.
Effects of splice sites on the intron retention in histamine H3 receptors from rats and mice

In the alternative splicing, intron retention, of histamine H3 receptors in rats and mice, the short transcript isoforms that are excised alternatively spliced introns are easily detected in a very low level in rats and are undetectable in mice using the regular PCR protocol. The retained introns have common 5' splice site and different 3' splice sites. The detailed mechanism for the special alternative splicing remains largely unclear. In this study, we developed a minigene splicing system to recapitulate natural alternative splicing of the receptors and investigated the effects of 5' and 3' splice sites on intron retention in HeLa cells. Mutating weak 5' and 3' splice sites of the alternatively spliced introns toward the canonical consensus sequences promoted the splicing of the corresponding introns in rat and mouse minigenes. The effect of splice site strength was context-dependent and much more significant for the 3' splice site of the longer alternative intron than for the 3' splice site of the shorter alternative intron and the common 5' splice sites; it was also more significant in the rat minigene than in the mouse minigene. Mutating the 3' splice site of the longer alternative intron resulted in almost complete splicing of the intron and made the corresponding isoform to become the nearly exclusive transcript in the rat minigene.
Mapping QTLs for heading synchrony in a doubled haploid population of rice in two environments

Simultaneous heading of plants within the same rice variety, also refer to heading synchrony, is an important factor that affects simultaneous ripening of the variety. Understanding of the genetic basis of heading synchrony may contribute to molecular breeding of rice with simultaneous heading and ripening. In the present study, a doubled haploid (DH) population, derived from a cross between Chunjiang 06 and TN1 was used to analyze quantitative trait locus (QTL) for heading synchrony related traits, i.e., early heading date (EHD), late heading date (LHD), heading asynchrony (HAS), and tiller number (PN). A total of 19 QTLs for four traits distributed on nine chromosomes were detected in two environments. One QTL, qHAS-8 for HAS, explained 27.7% of the phenotypic variation, co-located with the QTLs for EHD and LHD, but it was only significant under long-day conditions in Hangzhou, China. The other three QTLs, qHAS-6, qHAS-9, and qHAS-10, were identified under short-day conditions in Hainan, China, each of which explained about 11% of the phenotypic variation. Two of them,qHAS-6 and qHAS-9, were co-located with the QTLs for EHD and LHD. Two QTLs, qPN-4and qPN-5 for PN, were detected in Hangzhou, and qPN-5 was also detected in Hainan. However, none of them was co-located with QTLs for EHD, LHD, and HAS, suggesting that PN and HAS were controlled by different genetic factors. The results of this study can be useful in marker assisted breeding for improvement of heading synchrony.
Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice

Phosphorous (P) deficiency is a major restraint factor for crop production and plants have developed several mechanisms to adapt to low P stress. In this study, a set of 271 introgression lines (ILs) were used to characterize the responses of seedlings to low P availability and to identify QTLs for root traits, biomass, and plant height under P-deficiency and P-sufficiency conditions. Plant height, total dry weight, shoot dry weight, and root number were inhibited under P-deficiency, whereas maximum root length (MRL) and root-shoot ratio (RS) were induced by P-deficiency stress. Relative MRL (RMRL, the ratio of MRL under P-deficiency to MRL under P-sufficiency condition) and relative RS (RRS) were used to evaluate P-deficiency tolerance at the seedling stage. A total of 24 additive QTLs and 29 pairs of epistatic QTLs were detected, but only qRN4 was detected in both conditions. This suggested that different mechanisms may exist in both P supply levels. QTLs for adaptive traits (RMRL, RRS, RRV, and RRDW) and qRN4 consistently expressed to increase trait stability may contribute to P-deficiency tolerance. Twelve intervals were cluster regions of QTLs for P-deficiency tolerance, and one QTL (qRRS8) showed pleiotropic effects on P-deficiency tolerance and drought tolerance. These interesting QTLs can be used in marker-assisted breeding through the target ILs.
Mitochondrial tRNAGlu 14693G variant may modulate the phenotypic manifestation of deafness-associated 12S rRNA A1555G mutation in a Hah Chinese family

Mutations in mitochondrial 12S rRNA gene are one of the most important causes of aminoglycoside-induced and nonsyndromic hearing loss. Here we report the characterization of one Han Chinese pedigree with aminoglycoside-induced and nonsyndromic hearing loss.This Chinese family carrying the 12S rRNA A1555G mutation exhibited high penetrance and expressivity of hearing impairment. In particular, penetrances of hearing loss in this family pedigree were 43.8% and 25%, respectively, when aminoglycoside-induced heating loss was included or excluded. Mutational analysis of entire mitochondrial genomes in this family showed the homoplasmic A1555G mutation and a set of variants belonging to haplogroup Y2. Of these, the A14693G variant occurred at the extremely conserved nucleotide (conventional position 54) of the TψC-loop of tRNAGlu and was absent in 156 Chinese controls. Nucleotides at position 54 of tRNAs are often modified, thereby contributing to the structural formation and stabilization of functional tRNAs. Thus, the structural alteration of tRNA by the A14693G variant may lead to a failure in tRNA metabolism and impair mitochondrial protein synthesis, thereby worsening mitochondrial dysfunctions altered by the A1555G mutation. Therefore, the tRNAalu A14693G variant may have a potential modifier role in increasing the penetrance and expressivity of the deafness-associated AI555G mutation in this Chinese pedigree.
玉米花丝蛋白质组双向电泳条件的优化

以玉米温敏自交不亲和系'HE97'的花丝为材料,比较了3种不同蛋白质提取方法对双向电泳结果的影响,并对其中的蛋白质上样量、等电聚焦条件及SDS-PAGE凝胶浓度进行了探索与优化.结果表明,与酚提取法和改良的酚提取法相比,采用三氯乙酸/丙酮提取法提取蛋白质操作简便,所得的双向电泳图谱蛋白质点数较多,图谱背景清晰,是一种提取玉米花丝蛋白质的有效方法.优化后的双向电泳技术体系适合于玉米花丝全蛋白质的双向电泳分析.
牛奶子果实中蛋白质的提取和双向电泳分析方法的改良

比较2种提取牛奶子果实中总蛋白的方法以及用SDS-PAGE分析的结果表明,用酚法提取蛋白时,适当延长第2次平衡时间,并及时更换平衡缓冲液Ⅱ,可获得高分辨率的总蛋白图谱.这种改良了的方法解决了牛奶子果实中富含的色素和多酚等化合物对双向电泳(2-DE)的干扰问题,重复性好,适用于果实中蛋白的2-DE分析和尔后的技术操作.
穗花牡荆的组织培养

1 植物名称穗花牡荆(Vitex agnus-castus L.).2 材料类别幼龄植株的顶芽及带节茎段.3 培养条件基本培养基为WPM.(1)启动培养基:WPM+6-BA 0.5 mg·L~(-1)(单位下同)+NAA 0.2+3%蔗糖;(2)增殖培养基:WPM+6-BA 0.75+NAA 0.2+3%蔗糖;(3)壮苗及生根培养基:wPM+NAA 0.25+3%蔗糖.以上培养基中均加入5 g·L~(-1)琼脂,pH 5.8,常规高压灭菌.
柱前衍生-反相高效液相色谱法测定灰叶胡杨花粉中氨基酸的含量

胡杨、灰叶胡杨隶属于杨柳科杨属[1],是新疆干旱荒漠区植被的建群种和优势种,具有较强的抗逆特性[2].氨基酸的早期分析方法一般采用氨基酸分析仪,柱后衍生测定[3].
电感耦合等离子体原子发射光谱法测定钴酸锂材料中杂质元素

锂离子电池广泛应用于笔记本电脑、手机、DV机、数码相机等设备[1].钴酸锂是制备锂离子电池的正极材料,其中杂质元素的含量对正极材料的电性能有直接影响.因此,准确测定其杂质含量,对控制产品质量非常重要.本试验采用电感耦合等离子体发射光谱法(ICP-AES)测定钴酸锂中铁、镍、镁、钙、铜、钠、锰等7种杂质元素含量,采用基体匹配的方式,有效避免了基体干扰带来的测定误差.该方法具有简便、快速、灵敏、稳定、准确的特点[2],用于钴酸锂中杂质元素的测定,结果满意.
大众体育健身产品消费的中美比较研究

本文通过大众体育健身消费行为和消费产品中国和美国的比较研究,利用代蒙德模型家庭消费行为理论从不同角度分析了导致中美大众体育健身消费水平差异的深层原因:一国的经济发展水平;指出从提高消费水平激励的角度或是从总体提高消费者的购买能力的角度来讲,政府、健身行业,体育健身企业三方的互动很重要.
中小企业伦理道德体系建设——基于儒学伦理分析

本文通过对儒学伦理的分析,认为应用儒学伦理与现代企业经营理念和现行法律制度有机结合是建设中小企业伦理道德体系,解决中小企业伦理道德问题,保障中小企业可持续发展的有效方法.
企业经营者对宏观经济形势及改革热点的判断、评价和建议——2009年中国企业经营者问卷跟踪调查报告

本文是5016位企业经营者参与的"2009年中国企业经营者问卷跟踪调查"的调查报告的主要内容.调查显示,企业经营者认为2009年宏观经济形势逐渐企稳向好,企业景气状态温和回升,未来预期较为乐观.不过,当前金融危机的影响仍在延续,企业出口下滑明显,外需市场的恢复还需要一个过程,外需的萎缩导致部分行业产能过剩现象比较严重.同时,目前经济增长中政府投资驱动所产生的作用比较大,民间投资需求和消费需求尚未能充分启动,经济回升的结构是不平衡的,基础是不稳固的.面对企业发展的新困难、新挑战,企业经营者希望一方面通过加强自身管理、推动创新提升企业竞争力;另一方面建议决策部门及时调整宏观调控政策,进一步加快政府职能转变的步伐,健全社会保障体系,积极推进垄断性行业改革,为企业健康发展创造更加良好的外部环境.
系族控制人掏空与支持上市公司的博弈——基于复星系的案例研究

本文以中国最大的民营企业集团--复星系为例,研究系族企业控制人对旗下上市公司的掏空与支持行为.研究表明,复星系控制人--复星集团对旗下上市公司的掏空与支持是不对称存在的,有的是先掏空后支持,有的是持续性的掏空或支持,也有的是掏空和支持并举.在此基础上,进一步讨论了影响复星集团掏空与支持上市公司的关键因素,结果发现复星集团在权衡掏空与支持的选择时受到股权结构、入系方式、连锁董事、附加承诺等微观因素和行业特质、行业周期等中观因素以及法律规范、监管环境、投资者维权意识等宏观因素的影响.本文对客观认识系族控制人掏空与支持上市公司的逻辑关系提供了一个新思路.
输变电设备状态检修的应用

简要介绍了浙江省电力公司实施状态检修的阶段性成果,提出了实施状态检修的核心模型,论述了状态检修与坚强智能电网建设、设备资产全寿命周期管理、精益化管理等之间的关系,文章可为电网企业深入实施状态检修提供参考.
A Simulation of the Effect of Collisional Dissipation of Fast Electrons on the Radial Profiles of the Current Driven by Lower-Hybrid-Waves on the HT-7 Tokamak

For the effect of the collisional dissipation of fast electrons driven by the lower-hybrid waves, a predictive simulation is made for the HT-7 plasma. The simulation results show that the dissipation of fast electrons counteracts the effect of radial diffusion to some extent, thereby making the lower-hybrid driven current profile closer to the power deposition profile. So, in the case of an off-axis lower-hybrid wave power launching, the dissipation is helpful in maintaining a center-hollowed current profile in lower hybrid current drive (LHCD) plasmas, and thus possibly maintains the desired reversed magnetic shear.
Effect of Ion Concentrations on the Characteristics of a Multi-Component Magnetized Plasma Sheath

A hydrodynamic approach is used to investigate a three-component magnetized plasma sheath which consists of electrons and two species of positive ions. Assuming a phase space of one-dimensional spatial coordinate system and three-dimensional velocity coordinate system,the effect of different concentrations of positive ion species on some characteristics of the plasma sheath such as the velocity and density distribution of positive ion species and the electrostatic potential of this region is investigated. The calculated results show that the increase in the density ratio of positive ion species causes a decrease in both the ion velocities and the electrostatic potential of the sheath region. Also, it is shown that in the sheath region of a magnetized plasma consisting of only one positive ion species the bumps of the net density of charged particles disappears much faster. In addition, three-dimensional velocity of each positive ion species in the sheath region is plotted for different concentrations of positive ion species.
A biologically inspired model for pattern recognition

In this paper, a novel bionic model and its performance in pattern recognition are presented and discussed. The model is constructed from a bulb model and a three-layered cortical model, mimicking the main features of the olfactory system. The olfactory bulb and cortex models are connected by feedforward and feedback fibers with dis-tributed delays. The Breast Cancer Wisconsin dataset consisting of data from 683 patients divided into benign and malignant classes is used to demonstrate the capacity of the model to learn and recognize patterns, even when these are deformed versions of the originally learned patterns. The performance of the novel model was compared with three artificial neural networks (ANNs), a back-propagation network, a support vector machine classifier, and a radial basis function classifier. All the ANNs and the olfactory bionic model were tested in a benchmark study of a standard dataset. Experimental results show that the bionic olfactory system model can learn and classify patterns based on a small training set and a few learning trials to reflect biological intelligence to some extent.
多重聚合酶链反应联合芯片电泳在人乳头瘤病毒基因分型检测中的应用

研究证实HPV感染是CIN和宫颈癌的主要病因,而且不同HPV亚型感染的致病性和后果也有差异~([1-2]).HPV分型检测对临床CIN和宫颈癌筛查和评估、病程的进展监测、治疗后的随访、以及人群的流行病学状态和病毒疫苗的研制都有重要意义.目前用于临床的HPV检测技术主要有杂交法与实时荧光定量PCR~([3]),但这2种方法存在操作复杂、检测时间较长等不足.例如,导流杂交基因芯片技术即凯普导流杂交．HPV DNA检测法虽然能同时对多种HPV基因型进行分型检测,但操作繁琐、杂交时间较长~([4-5]).芯片电泳(microchip electrophoresis)具有快速分离和低样品消耗量等优点~([6]),目前已广泛应用于多种类型基因分析研究~([7]).本研究建立一种基于芯片电泳的HPV快速分型方法,为临床筛查提供依据.
家族性近指间关节融合症一组

患儿男,46 d.出生后家人发现除双手拇指外,其余各指中节指间关节活动受限而来我院就诊.专科检查:双手示、中、环、小指近指间关节的主、被动活动受限,近指间关节部位皮纹消失,身体其他部位未见异常.
高糖甘蔗新品种(系)区试及生产示范研究

2008年在贞丰、望谟坝地和坡地采用引进甘蔗新品种粤糖00/236、桂引9号、桂糖00/122、96/154、96/211进行区试和生产示范,以F 134为对照,经1年新植结果表明:5个品种农艺性状、经济性状和抗性均优于对照,坝坡地平均蔗糖分含量比对照增长1.79～3.43个百分点,甘蔗产量和产糖量分别比对照增长52.5%～123.2%和90.8%～166.1%,增产增糖效果极显著.粤糖00/236,桂糖00/122和桂糖96/154蔗糖分含量迭16.0%以上,增产增糖55.7%～82.0%、74.5%～120.7%,可作为高糖品种应用,桂糖96/211蔗糖分含量15.0%以上,增产增糖104.8%和137.3%,可作高产高糖品种应用.生产示范面积17 hm2,蔗糖分比对照增长1.48～3.32个百分点,产量和产糖量分剔比对照增长42.3%～104.8%和56.5%～137.3%,示范效果明显.
BRS-3促效剂——一种可能治疗肥胖的新药

肥胖已成为世界性流行病,但至今尚无一种令人满意的治疗药物.铃蟾素(bombesin,亦译蛙皮素)受体亚型3(BRS-3)是铃蟾素受体亚家族的一员,其另二个成员已经详细研究.而BRS-3的自然配体尚不清,实际上它和铃蟾索的结合力很低.已知有些合成或自然肽能活化BRS-3,但作用很弱且为非特异性结合.初步研究认为它和代谢率、肥胖及胰岛素抵抗有关.
原发性肝癌合并高钙血症危象一例

2008年11月,福建中医学院附属厦门市中医院肝病中心肝外科收治1例原发性肝癌血钙高达4.4 mmol/L并发昏迷的病人,经积极抢救,获得成功.现将诊治经过报告如下.
内镜超声引导细针穿刺诊断后纵隔占位病变2例

内镜超声(EUS)对后纵隔病变的定位、定性诊断有特殊意义.EUS引导下细针穿刺活检术(EUS-FNA)能直接取得病理学诊断依据,明确邻近食管周围纵隔占位病变、肿大淋巴结等的性质.本研究应用EUS-FNA诊断2例性质不明的后纵隔占位病变,报告如下.
On the bounds of frequency-selective channel capacity with doubly correlated geometrical MIMO channel model

The multi-input multi-output (MIMO) technology plays an important role in link transmissions. This article considers the general case for the ergodic capacity in doubly correlated frequency-selective MIMO channel. In the study, the geometrical MIMO channel model is presented. Based on the formula of MIMO ergodic capacity, the capacity limits are studied with arbitrary finite number of antennas in the frequency-selective MIMO channel. It first derives the exact expressions for the upper bound and lower bound in doubly correlated MIMO channel. The results for the single-ended correlation and independent identically distributed (i.i.d.) MIMO channel are also obtained as special cases. Then the simple expressions of the capacity bounds are attained at high SNR. Finally, results are provided by Monte Carlo simulations to verify the tightness of the derived bounds.
TKTL1在胃癌中的表达及其意义

目的 探讨转酮醇酶1(TKTL1)在胃癌组织中的表达及其意义.方法 采用免疫组化SP法检测TKTL1蛋白在胃癌组织、癌旁组织和正常胃黏膜组织中的表达情况,分析它们与胃癌临床病理特征之间的关系.结果  TKTL1在胃癌组织中呈高表达(P<0.01),其高表达与胃癌的浸润深度、肿瘤TNM分期以及淋巴结转移有关;上述3特征的分组间差异均有统计学意义(P<0.05).结论  TKTL1的高表达与胃癌的发展和转移关系密切.
2009年中国儿童保健状况

1 我国儿童健康状况我国儿童健康状况我国人口众多,中国儿童人群约占伞国总人口的1/3(约3.42亿),以农村地区为主[1].2009年我国儿童保健事业持续发展,约2/3婴幼儿和学龄前儿童(<3岁和<7岁)已进入儿童系统管理[2],儿奄健康状况显著改善.
电吸附除盐工艺处理城市再生水的中试研究

通过电吸附除盐中试,考察了对城市再生水进一步除盐的可行性.结果表明,电吸附除盐工艺对氯化物和离子的平均去除率分别为90%和78%,产水率为76%,电耗为0.57 kW·h/m3,且其尾水的COD浓缩不明显,可直接外排;该工艺自控程度高、运行操作简单、出水水质稳定、除盐效果明显且直接运行成本较低,用于城市再生水的深度处理是可行的.
旋毛虫感染兔唾液中抗旋毛虫IgG抗体水平

将28只日本大耳兔随机分为实验组(20只)和对照组(8只),实验组用旋毛虫脱囊幼虫经口灌胃日本大耳兔(3 000条/只),对照组不做任何处理.采集感染前和感染后1～6周兔唾液和血清以及对照组兔唾液和血清.建立旋毛虫肌肉幼虫排泄分泌抗原(MLESA)为诊断抗原的间接ELISA,测定兔唾液和血清中抗旋毛虫IgG抗体.结果 显示,感染后1～6周,唾液阳性率分别为10%、15%、40%、65%、85%和95%;血清阳性率分别为35%、50%、80%、90%、100%和100%.感染后1～3周,唾液阳性率与血清阳性率差异有统计学意义(χ2=3.58、5.23、6.67, P＜0.05),感染后4～6周,两者差异无统计学差异(χ2=0.12、1.03、1.03,P＞0.05).提示在血清标本采集困难的情况下,MLESA的间接ELISA法检测唾液中抗旋毛虫IgG抗体可作为旋毛虫病免疫诊断的辅助方法.
肺炎链球菌L型对人淋巴细胞因子分泌刺激作用

目的 观察细胞壁缺陷肺炎链球菌刺激人淋巴细胞分泌白细胞介素-1β(IL-1β)与肿瘤坏死因子-α(TNF-α)的作用.方法 用青霉素诱导肺炎链球菌形成L型,分离稳定L型纯培养物.将热灭活的肺炎链球菌的细菌型及稳定L型菌液分别与人淋巴细胞混合,体外37℃培养24 h,用酶联免疫吸附试验(ELISA)检测培养物上清中IL-1β和TNF-α含量.结果 L型肺型炎链球菌处理的人淋巴细胞培养物上清中IL-1β含量为(29.303±2.812)～(69.894±2.358)pg/mL、TNF-α含量为(38.467±2.208)～(74.329±2.283)pg/mL,明显低于其亲代细菌型肺炎链球菌处理的人淋巴细胞(P<0.05),且与菌液浓度呈相关性(r分别为0.969和0.960).结论 肺炎链球菌L型仍然具有刺激人淋巴细胞分泌IL-1β和TNF-α的免疫原性，但较其亲代细菌型的免疫原性降低.
流动妇女人格特征与心理健康关系

目的 探讨流动妇女人格特征与心理健康的关系.方法 2009年3月,采用艾森克人格问卷(Eysenck Personality Questionnaire,EPQ)和症状自评量表(Symptom Checklist 90,SCL-90),通过判断抽样的方法在江苏省江阴市和张家港市对656名流动妇女进行问卷调查.结果 女性流动人口在内外向(E)、掩饰性(L)分量表得分均值高于全国女性常模(P<0.01),神经质(N)分量表得分均值低于全国常模(P<0.01);N,L分量表得分均值高于流动男性(P<0.01),精神质(P)分量表得分均值低于流动男性(P<0.05);EPQ中P分量表、N分量表与SCL-90总分及各因子分呈正相关;E分量表与SCL-90总分及各因子分呈负相关,N分量表与SCL-90总分和各因子分相关系数最高;回归分析表明:精神质、内外向和神经质是影响流动妇女心理健康状况的重要人格特征.结论 流动妇女人格特征和心理健康状况呈一定程度的相关.
A Phase Ⅱ Trial of Gefitinib as Maintenance Therapy after First-Line Chemotherapy for Advanced Non-Small Cell Lung Cancer in China

Objective:To investigate the efficacy and safety of gefitinib as maintenance therapy for advanced non-small cell lung cancer (NSCLC) patients who obtained disease control (DC) after first-line chemotherapy in Chinese population.  Methods:Chinese patients with advanced NSCLC treated with standard chemotherapy and obtained DC were assigned to receive gefitinib as maintenance treatment. The primary end point was overall survival time (OS), the second end point was disease control rate (DCR) and progression-free survival time (PFS). DCR included complete response (CR) plus partial response (PR) and plus stable disease (SD). The impact of epidermal growth factor receptor (EGFR) mutation status on the treatment as exploratory point was also evaluated by denaturing high-performance liquid chromatography (DHPLC).  Results:Among 75 enrolled patients, the overall response rate was 37% and the DCR (CR + PR +SD) was 66%. The median PFS and OS were 17.13 months and 26.13 months respectively, with 1- and 2-year survival rates 89.3% and 34.7%. Patients harboring somatic EGFR mutations obtained a prolonged median PFS and OS compared with EGFR wide type (25.1 vs. 13.0 months, P=0.019 and 33.37 vs. 25.57 months, P=0.014, respectively). In COX regression model, only EGFR mutation status was the independently factor influencing both PFS and OS (P=0.029 and 0.017, respectively), however, rash status was the predictor in terms of PFS (P=0.027).Conclusion:Gefitinib produced encouraging survival when delivered as maintenance therapy in Chinese patients obtaining DC after first-line chemotherapy, especially for patients carrying somatic EGFR mutations. EGFR mutation is an independently predictive factor of survival.
不同术式鼓室成形术后患者的听力分析

目的 探讨不同术式鼓室成形术对中耳增益的影响.方法 研究对象为135例(137耳)慢性化脓性中耳炎患者,根据手术类型分为伴乳突切除术的鼓室成形术26例(26耳,I组)、不伴乳突切除术的鼓事成形术61例(62耳,II组)和单纯鼓膜修补术48例(49耳,III组)三组.比较三组患者干耳后平均听阈及各频率听阈改善情况,计算各组的中耳危险指数(middle ear risk index,MERI)值,分析MERI值与术后疗效的相关性.结果 II组较I组患者术后平均听阈明显降低(P<0.05),低频听力改善前者优于后者(P<0.05),II组与III组患者术后平均听阈及各频率听力提高值差异无统计学意义(P>0.05),各组术后听力改善与MERI值无明显相关性.结论 不伴乳突切除术的鼓室成形术对中耳增益效应影响显著,术后患者听功能改善明显,鼓室成形术后疗效与MERI值无显著相关性.
不同部位起搏的即刻体液因子变化

安置永久起搏器的患者经常表现为单一的传导系统障碍而无其他心脏疾患,但部分患者会出现心功能不全或心律失常.既往的观察多局限在单心室(VVI)起搏和双腔顺序(DDD)起搏(均为右室心尖起搏)2种方式的比较,本研究通过测定不同心室起搏部位体液因子含量的变化,分析起搏部位对心脏功能的影响.
Cadna/A在货运铁路专线噪声预测评价中的应用

Cadna/A是基于德国RLS90通用计算模型的噪声模拟软件,其计算原理源于ISO标准9613-2:1996<户外声传播的衰减的计算方法>,广泛用于环境评价、建筑设计、交通管理、城市规划等众多领域.采用德国Datakustik公司出品的Cadna/A软件,模拟预测了新建铁路交通噪声对周围环境的影响,并提出了噪声污染的控制措施.
妊娠相关血浆蛋白A定量测定方法的建立

目的:建立人外周血妊娠相关血浆蛋白A(PAPP-A)定量酶联免疫测定方法.方法:以生物素化牛血清白蛋白-链霉亲和素间接包被微孔板,将标记有生物素的抗PAPP-A抗体作为捕获抗体,结合标本中的抗原成分;标记有辣根过氧化物酶的抗PAPP-A抗体作为检测抗体,经酶催化底物显色,定量测定人体外周血PAPP-A.结果:所建立的测定方法灵敏度为0.31 mg/L,回收率均在95%～100%之间;该方法线性、稳定性及特异性良好;3份低、中、高浓度的血清标本重复测定10次,批内变异系数(CV)均小于10%,批间CV均小于15%;该方法测量结果与国外同类方法测量结果具有相关性(r=0.982,P<0.05),2种检测方法高度相关.结论:本文采用间接包被技术所建立的人外周血PAPPA固相酶联免疫测定方法灵敏、可靠、稳定性好,与国外同类方法的试剂盒之间相关性较高.
子宫内膜样腺癌组织中NF-κB p65与ERα的表达及意义

目的:探讨核因子(NF)-κB p65蛋白在不同子宫内膜组织中的表达及其与雌激素受体(ER)α蛋白之间的关系.方法:应用免疫组织化学二步法检测10例正常子宫内膜、60例子宫内膜增生症、20例子宫内膜样腺癌中NF-κB p65蛋白、ERα蛋白的表达,并分析NF-κB 065和ERa蛋白在3组中的表达情况.结果:在正常子宫内膜、子宫内膜增生症及子宫内膜样腺癌中NF-κB p65蛋白中位表达率分别为0、0.47%及9.13%,ERα蛋白平均表达率分别为5.25%、49.97%及35.01%,差别均有统计学意义(P<0.05或P<0.01).在子宫内膜增生类型为单纯性增生、复杂性增生及非典型增生的组织中,NF-κB p65蛋白中位表达率分别为0、1.27%及3.49%,差别均有统计学意义;ERα蛋白平均表达率分别为60.81%、52.30%及36.81%,单纯性增生组与非典型增生组、复杂性增生组与非典型增生组之间差别均有统计学意义(P<0.05或P<0.01).在内膜样腺癌中,NF-κBp65与ERa表达呈负相关(r=-0.491,P:0.028).结论:NF-κB p65蛋白与ERα蛋白在子宫内膜增生症及子宫内膜样腺癌组织中表达异常,可能促进了子宫内膜样腺癌的形成和进展.
广梅汕铁路电气化改造的生态效应分析

定量地分析了广梅汕铁路电气化改造工程的生态效应,主要探讨了改造过程中生物量损失、水土流失的生态负效应及改造后水环境、大气环境得到改善的生态正效应.结果表明,广梅汕铁路电气化改造施工造成的植被生物量损失共计334.5 t ,工程造成新增水土流失量为689.0 t .电气化改造后,龙川机务段每年生产污水的污染物排放数量将会减少,其中油类将减少21.9 kg ,COD 减少76.1 kg ,SS 减少145.3 kg .经过电气化改造,全线每年减少耗油2.16 万t ,每年向大气减少排放153.21 t CO 、1 085.42 t NOX 、47.47 t SO2 、328 t 碳烟和110.06 t Cn Hm .随着植被恢复措施功能的显现,其生态负效应是逐年减弱的,因而该线路电气化改造工程的生态正效应远远大于其负效应,改造工程会带来良好的生态效应.
苏州河浮游植物群落恢复的生境条件研究

于2005年4月至2006年5月期间对苏州河浮游植物群落及理化因子进行研究,分析了苏州河浮游植物现状及水质现状,并分析两者之间的关系,得出水温、总磷、总氮、CODcr 等理化因子对浮游植物群落有显著影响,且其影响均存在临界值,分别为水温15 ℃、总磷0.6 mg ·L-1 、总氮10 mg ·L-1 、CODcr 20 mg ·L-1 .即在苏州河的浮游植物群落恢复中,当理化因子小于其相对浮游植物群落的临界值时,小球藻的出现频率低、密度百分比低,梅尼小环藻出现频率高、密度百分比高;而当理化因子大于其相对浮游植物群落的临界值时,小球藻的出现频率高,密度百分比高,梅尼小环藻出现频率低,密度百分比低.因此在对苏州河进行生态恢复时,应考虑到各理化因子对浮游植物影响的临界值,综合权衡各方面因素,确定水质恢复应达到的目标.
空间矢量脉宽调制中电压矢量最优化算法研究

在交流伺服系统电流控制的空间矢量脉宽调制中,通常的电压指令限制算法会导致母线电压不能被充分利用,并产生电压矢量方向误差.针对此问题,该文提出了基于线电压限制和相电压中心点平移的最优化算法.一个电角度周期内的分析结果证明,在63.3%的角度区间,最优算法处理后的电压矢量模大于通常方法,母线电压得到充分利用,且没有方向误差.
卡尔曼滤波在GPS制导火箭弹中的应用

针对直接采用全球定位系统(GPS)测量弹道误差较大的问题,该文提出应用扩展卡尔曼滤波方法进行弹道测量.采用火箭弹弹道模型和GPS误差模型,建立卡尔曼滤波系统状态模型和以伪距为观测量的系统测量模型,推导并分析了相应的滤波公式,并对GPS动态定位的数据进行滤波.理论分析和仿真结果表明,GPS测量的位置误差和速度误差具有随机性,且误差幅度较大,采用卡尔曼滤波算法后,弹道的位置估计误差和速度估计误差分别降低到观测误差的1/3和1/4左右,而且滤波收敛速度快.