mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
去甲肾上腺素水平与孔源性视网膜脱离视网膜下液的关系

目的测定孔源性视网膜脱离患者术前与术后血浆中去甲肾上腺素（NE）浓度探讨其与视网膜下液吸收是否有关。方法收集我科孔源性视网膜脱离的病例30例,检测患者术前、术后血浆中NE水平,观察其术后视网膜下液吸收时间,按视网膜下液吸收的时间分为A（≤3 d）、B（47 d）及C（>7 d）三组,比较三组患者术前、术后第1天、术后不同性别组NE水平。结果术前三组NE水平比较:A组高于B组,差异有统计学意义,B组低于C组,差异有统计学意义;各组术后第1天与术前NE水平比较,C组NE水平较高,差异有统计学意义;C组术后NE升高值的比较,男性高于女性。结论 NE水平与视网膜脱离患者术后视网膜下液吸收具有相关性。视网膜脱离术后视网膜下液吸收慢的患者血浆中NE水平高。
养老服务社会化的困境与出路

社会福利社会化,是21世纪中国社会福利事业改革和发展的战略思路和基本任务,养老服务社会化是其重要组成部分。为此,民政部等11部委于2000年颁布了《关于加快实现社会福利社会化的意见》(国办发[2000]19号),2005年民政
放疗同期胃镜下植入5-FU缓释粒子治疗老年食管癌21例疗效观察

80%的食管癌患者临床确诊时,局部病灶已经广泛浸润和(或)远处转移,失去了根治性切除的机会。老年食管癌患者因体质和年龄问题对联合治疗耐受性差,以放射治疗为主,但放疗的局部控制率与远期生存率都较低,总体治疗效果差,5
妥洛特罗贴剂治疗老年慢性阻塞性肺疾病患者急性加重期的疗效观察

目的观察妥洛特罗贴剂治疗COPD急性加重期的疗效和安全性。方法 120例COPD急性加重期的患者随机分为2组:试验组和对照组,试验组给予妥洛特罗贴剂2 mg,每日1贴,同时吸入丙酸氟替卡松250μg/次,每2次/d,对照组吸入沙美特罗/氟替卡松（50/250μg）,每日2吸。结果试验组治疗后的肺功能、临床症状均显著改善,缓解药的使用及夜间憋醒次数减少。试验组使用缓解药的中位数为1次,95%CI为06次,较对照组（中位数为2次,95%CI为028次）明显减少（t=-3.652,P<0.01）;试验组夜间憋醒次数（1.3±1.2）少于对照组（2.3±1.4）（t=-5.324,P<0.01）;试验后试验组的FEV1为（1.19±0.03）L,较试验前提高了9.1%,明显高于对照组（1.09±0.02）L（t=-11.46,P<0.01）;试验组在第2周以后呼气峰流量（PEF）为（225.4±55）L/min,较试验前提高了7.4%,明显高于对照组[（212.4±59.1）L/min]（t=-10.313,P<0.001）;试验组住院时间（14.01±1.03）d,短于对照组[（18.75±1.71）d]（t=-10.64,P<0.05）。结论使用妥洛特罗贴剂治疗COPD急性加重期,可以控制患者的临床症状并改善肺功能,是安全有效的。
关于给水管道优化设计的几点思考

在满足各种约束条件的前提下,实现管网最大可靠度及最小投资和运行年费用折算值,一直是给水领域研究热点之一。结合实际案例,着重分析了经济流速的合理确定、多目标函数的建立及低粗糙系数内防腐涂层的选取对给水管道优化设计的意义。
ABR—SBR—接触氧化—气浮工艺处理纤维板生产废水

人造纤维板厂废水中纤维素含量较高,可生化性差,难以处理达标。采用ABR—SBR—接触氧化—气浮工艺处理某纤维板厂生产废水,工程实践表明,在进水CODCr10 552mg/L、NH3—N380mg/L、SS 784mg/L、pH 4.8时,出水CODCr88mg/L、NH3—N 5mg/L、SS 10mg/L、pH 6.8,各项指标均达到《污水综合排放标准》(GB 8978—1996)一级标准,取得较好的社会和经济效益。
输尿管血管瘤一例

血管瘤多好发于肝、脾等血管丰富的器官和组织,较少发生于中空性器官,而发生于输尿管者更少见。输尿管血管瘤发生于间叶组织,可能与胚胎时中肾管形成过程发育异常有关,很少累及黏膜,内外肌层呈分离状态。输尿管血管瘤起病隐匿,最常见的症状为偶有肉眼血尿。本例患者临床上尿常规镜下潜血试验阳性。
常用农药对5种昆虫细胞系的毒力测定及分析

自20世纪60年代Grace建立了第一个能够稳定传代的昆虫细胞系以来(Grace，1962)，经过几十年的研究和发展，昆虫细胞培养已在细胞系建立、培养技术和方法、昆虫细胞培养技术的利用等方面取得了很大的进展，昆虫细胞被广泛地应用于医学、农学及生物学的各个领域(宋德伟等，2004;张佑红等，2006;Maramorosch et al.，1992)。目前，全世界已有500多株昆虫细胞系得以建立(Lynn，2001)，100多种昆虫细胞被应用于生物学和医学的研究(程东美等，2002)，利用昆虫细胞检测化学药物及生物活性物质毒力的立体毒力测试体系也逐步发展起来。从20世纪60年代开始就有学者进行了昆虫细胞离体条件下农药的毒力检测(Mitsuhashi et al.，1970;杨红等，1996)，Waterhouse(1965)首次研究了DDT对天蚕蛾传代细胞系的影响;Yoshida(1979)研究了有机合成的杀虫剂对离体细胞正常呼吸代谢及生物大分子合成的影响;Stipanovic等(1990)首次采用了MTT法测定了棉酚系列天然产物对烟芽夜蛾(Heliothis viresecn)细胞系的毒力;Rahiouid等(2007)、Salehzadeh等(2002)报道了蛋白及农药等对草地夜蛾(Spodoptera frugiperda)细胞系Sf－9的细胞毒性;另外，Das等(2006)也利用农药对人体外周血淋巴细胞系的毒力来检测农药对人体的影响。国内在这方面的研究开始得较晚，且大多数集中于利用MTT法检测，毛黎娟等(2005)测定了杀虫剂对家蚕细胞的毒力;钟国华等(2008a)研究报道了9种植物源物质对斜纹夜蛾(Prodenia litura)细胞系SL－1细胞凋亡小体的影响;周青春等(1997a;1997b)用改进的MTT法检测了丙酮、乙酸乙酯、二甲苯等有机溶剂杀虫剂对菜粉蝶(Pieris rapae)细胞系和棉铃虫(Helicoverpa amigera)细胞系的毒力;杨红等(1996)利用菜青虫(菜粉蝶)细胞检测二甲基砜、丙酮、乙酸乙酯及二甲苯等的毒力。
微藻化感作用及化感物质在赤潮演替中的作用

近年来,随着海洋微藻群落生态和种群生态研究的深入,微藻群落组成的演替及种间关系愈来愈受到重视,成为现代海洋浮游植物生理生态学和化学生态学研究的热点[1-3]。微藻群落演替是一个比较复杂的动态变化过程,涉及的驱动因素比较多,因
基于DIFA的动态非高斯过程监控方法及其应用

近年来,随着生产过程结构日益复杂,过程变量与采样数据、数据间耦合关系均日渐增强,企业对于产品质量以及生产过程安全的要求日渐提高,对过程监控技术的改进与革新也日益重视,基于数据驱动的多元统计过程监控方法(MSPC)因其避免构建复杂机理模型、充分利用过程采样数据而受到学术界及工业界的广泛关注,并在化工过程中取得了成功应用[1-5]。以主元分析(PCA)、偏最小二乘(PLS)、因子分析(FA)为代表的传统统计监控理论假设生产过程数据为静态,且变量满足多元高斯分布。然而,实际生产中由于闭环控制环节的存在,以及系统惯性等诸多因素的影响,过程变量往往不完全满足高斯分布,且变量本身不同采样时刻数据亦存在时间序列相关性,仍然采用传统多变量统计方法对生产过程实施监控,势必导致误报、漏报的频繁发生。因此,如何处理工业过程动态特性以及过程变量非高斯分布的问题成为当前研究热点之一。
快速康复外科对肝脏手术患者术后康复状态的影响

目的探讨快速康复外科(FTS)对肝脏手术患者术后康复状态的影响。方法原发性肝癌患者96例,根据围手术期处理情况分为两组:快速康复组(FTS组)49例,运用快速康复外科理念护理;对照组47例,给予常规围手术期护理。比较两组患者术后康复状态。结果 FTS组术后康复状态较对照组改善明显,行不规则肝切除术、术中出血量较小、术中未输血的患者术后早期康复状态改善明显(P<0.05);手术3 d后FTS组行规则肝切除、术中输血的患者较对照组恢复好。结论 FTS应用于肝脏外科围手术期能改善术后早期康复状态,在手术创伤较小、患者一般情况较好的情况下术后康复改善显著。
西安地区RhD阴性个体RHD 711D~(el)C等位基因频率研究

目的研究西安地区RHD基因711Del C等位基因分布状况。方法应用PCR方法检测RhD阴性个体,对特殊标本进行测序分析。结果在1 492名RhD阴性个体中,检出RHD 711Del C等位基因8例(占0.54%),且均为E抗原阳性。结论获得了西安地区RHD 711Del C基因分布的初步状况,RHD基因711Del C的突变可能与E抗原有相关性。
Hawking radiation from a Vaidya black hole by Hamilton-Jacobi method

Using the Hamilton-Jacobi method, Hawking radiation from the apparent horizon of a dynamical Vaidya black hole is calculated. The black hole thermodynamics can be built successfully on the apparent horizon. If a relativistic perturbation is given to the apparent horizon, a similar calculation can also lead to a purely thermal spectrum, which corresponds to a modified temperature from the former. The first law of thermodynamics can also be constructed successfully at a new supersurface which has a small deviation from the apparent horizon. When the event horizon is thought as such a deviation from the apparent horizon, the expressions of the characteristic position and temperature are consistent with the previous result that asserts that thermodynamics should be built on the event horizon. It is concluded that the thermodynamics should be constructed on the apparent horizon exactly while the event horizon thermodynamics is just one of the perturbations near the apparent horizon
Laboratory study on the formation of Al_2O_3 inclusions at the on-set of deoxidation and during reoxidation

The formation of Al_2O_3 inclusions in liuid iron has been investigated in a resistance heated tube furnace to obtain deoxidation and reoxidation related data. The formation of inclusions during the early stages of deoxidation was simulated by bringing a piece of Al in contact for a short time with liquid Fe containing different dissolved oxygen levels. Reoxidation was studied by exposing Al containing Fe melts to a CO/CO_2 atmosphere. Through modeling, an extimate of the local and time-dependent growth conditions for the inclusions can be made and linked to the inclusion characteristics
High pressure X-ray diffraction study of SrMnO3 perovskite

Using a diamond anvil cell device and synchrotron radiation, the in-situ high-pressure structure of SrMnO3 has been investigated. At pressure up to 28.6 GPa, no pressure-induced phase transition is observed. The lattice parameters as a function of pressure is reported, and the relationship of the axial compression coefficients is β_a > β_c. The isothermal bulk modulus K_(298)=266(4) GPa is also obtained by fitting the pressurevolume data using the Murnaghan equation of state
Transverse radius dependence for transverse velocity and elliptic flow in intermediate energy HIC

The mean transverse velocity and elliptic flow of light fragments (A < 2) as a function of transverse radius are studied for 25 MeV/nucleon ~(64)Cu+~(64)Cu collisions with impact parameters 3-5 fm by the isospindependent quantum molecular dynamics model. By comparison between the in-plane and the out-of-plane transverse velocities, the elliptic flow dependence on the transverse radius can be understood qualitatively, and variation of the direction of the resultant force on the fragments can be investigated qualitatively
儿童过渡型肝细胞肿瘤体外切除一例

临床资料 患儿男, 13岁. 因右上腹巨大包块1个月余于2010年7月 18日入院. 2010年6月27日, 患儿家长偶然触及其右上腹一直径约15 cm包块, 无触痛, 不伴腹痛、腹胀, 无畏寒、发热, 无潮热、盗汗, 无头昏、头痛, 无反酸、嗳气, 无心慌、心累, 无皮肤、巩膜黄染, 无尿频、尿急、尿痛, 元腹泻、便血、陶土便等. 2010年7月12日, 当地医院CT检查提示肝左叶巨大包块, AFP742.7μg/L, ALT 54 U/L, AST 57 U/L, ALP 270 U/L, TBil 20.2μmol/L, DBil 8.6μmoL/L, IBil 11.6μmol/L. 诊断为肝母细胞瘤, 未做其他处理. 随后包块进行性增大, 为进一步治疗入住我院. 患儿精神、睡眠、饮食良好, 大小便正常, 体质量无明显下降
Distance metric learning guided adaptive subspace semi-supervised clustering

Most existing semi-supervised clustering algorithms are not designed for handling highdimensional data. On the other hand, semi-supervised dimensionality reduction methods may not necessarily improve the clustering performance, due to the fact that the inherent relationship between subspace selection and clustering is ignored. In order to mitigate the above problems, we present a semi-supervised clustering algorithm using adaptive distance metric learning (SCADM) which performs semi-supervised clustering and distance metric learning simultaneously. SCADM applies the clustering results to learn a distance metric and then projects the data onto a low-dimensional space where the separability of the data is maximized. Experimental results on real-world data sets show that the proposed method can effectively deal with high-dimensional data and provides an appealing clustering performance
Inundative field releases and evaluation of three predators for Bemisia tabaci (Hemiptera: Aleyrodidae) management in three vegetable crops

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a global pest on numerous crops, including vegetables. Weekly inundative releases of a coccinellid predator (Coccinella undecimpunctata L. [Coleoptera: Coccinellidae]), a common green lacewing predator (Chrysoperla carnea Stephen [Neuroptera: Chrysopidae]), and a mirid predator (Macrolophus caliginosus [Wagner] [Hemiptera: Miridae]) were independently made in three vegetable crops (cabbage [Brassica oleracea var. capitata L.], cucumber [Cucumis sativus L.], and squash [Cucurbita pepo L.]) for the management of the sweetpotato whitefly. Approximately 1 million to 2.5 million larvae or nymphs of each predator were released in the vegetable crops during 20 weeks. Whitefly populations were reduced by ≈ 25%-45% during most of the season in each crop where each predator was released. The effect of each predator was similar on whitefly population reduction. Late in the season (October) when whitefly populations were low, generally no benefit was obtained from releasing the predators. Numbers of predators recovered during sampling in all crops were greatest for C. carnea, but this corresponded with the fact that more individuals of this predator were released than any other predator in the experiment. These results help define the utility of these natural enemies for managing B. tabaci in vegetable crops
Identification of Target Ligands of CORYNE in Arabidopsis by Phage Display Library

CORYNE (CRN) plays important roles in stem cell division and differentiation of shoot apical meristem(SAM) in Arabidopsis thaliana. The cytoplasmic kinase domain of CRN has been cloned and expressed in Escherichia coli, and further purified by two consecutive steps of affinity chromatography. By using this purified CRN as a ligand, a 12-mer random-peptide library was used to determine the specific amino acid sequences binding with the recombinant CRN molecule. After four rounds of biopanning, positive phage clones were isolated and sequenced, and further tested by enzyme linked immunosorbent assay for their binding ability and specificity. Two positive clones that specifically bind to the intracellular protein kinase domain of CRN have been identified. Alignment of these peptides and the kinase-associated protein phosphatase (KAPP) shows high similarity, indicating that KAPP might interact with the cytoplasmic kinase domain of CRN and negatively regulate the CLV signal. Our current study would be helpful to better understand the CLV3 signal pathway
单纯后路颈椎椎弓根钉棒系统内固定治疗下颈椎骨折脱位

目的探讨单纯应用颈椎椎弓根钉棒系统内固定技术治疗下颈椎骨折脱位的疗效. 方法对2005年8月至2009年10月应用单纯颈椎椎弓根钉棒系统内固定治疗的19例下颈椎骨折脱位患者资料进行回顾性分析, 其中男16例, 女3例; 年龄23~62岁, 平均43岁. 7例为严重下颈椎骨折脱位, 3例为陈旧性下颈椎骨折脱位, 9例为伴有后柱骨折或关节突绞索Ⅰ~Ⅱ度的下颈椎骨折脱位. 结果全部患者均获良好复位、固定, 共成功置人82枚椎弓根螺钉, 术后X线片及CT片示除9枚螺钉发生偏移外, 其余73枚螺钉完全位于椎弓根内. 18例患者术后获6~45个月(平均14个月)随访. 按Frankel分级: A级 4例, 均无好转, 但截瘫平面均有不同程度下降, 上肢感觉、运动功能明显改善; 3例B级转为E级; 其余患者均恢复至正常. 所有患者均获良好骨性融合, 未发现断钉、断棒及脱钉现象. 结论应用椎弓根钉棒内固定系统治疗颈椎后柱损伤为主的下颈椎骨折脱位, 有利于颈椎有效复位和早期康复, 可早期实现三柱固定, 同时可行减压治疗
A SINGULAR BIOECONOMIC MODEL WITH DIFFUSION AND TIME DELAY

This paper studies a prey-predator singular bioeconomic system with time delay and diffusion, which is described by differential-algebraic equations. For this system without diffusion, there exist three bifurcation phenomena: Transcritical bifurcation, singularity induced bifurcation, and Hopf bifurcation. Compared with other biological systems described by differential equations, singularity induced bifurcation only occurs in singular system and usually links with the expansion of population. When the diffusion is present, it is shown that the positive equilibrium point loses its stability at some critical values of diffusion rate and periodic oscillations occur due to the increase of time delay. Furthermore, numerical simulations illustrate the effectiveness of results and the related biological implications are discussed
ADAPTIVE SET STABILIZATION OF THE ATTITUDE OF A RIGID SPACECRAFT WITHOUT ANGULAR VELOCITY MEASUREMENTS

The global adaptive set stabilization problem of the attitude of a rigid spacecraft is addressed in this paper. Two different cases are considered. First, by using adaptive backstepping method, the authors design a global adaptive control law for the attitude control system with unknown inertia matrix such that the attitude and the angular velocities can be globally asymptotically stabilized to a set consisting of two equilibria. And then, based on the obtained backstepping adaptive law, the authors consider the case that the angular velocities are not measurable. By introducing an auxiliary state, a semi-global adaptive set stabilization law without angular velocity measurements is also designed. It is rigorously proved that, for the two cases, both of the closed loop systems satisfy the set stability. The effectiveness of the proposed methods is verified by simulation results
NO与精子及其获能关系的研究现状

一氧化氮(NO)是一种微小的、可扩散、多功能的跨细胞信使, 已被证实在人体多数器官、组织的许多生理和病理过程中发挥重要作用. NO既是一种有害气体, 又是一种生物活性物质. NO对生殖活动具有十分重要的作用, 如对睾丸微循环的调节, 参与睾酮分泌, 对生精过程、精子活力和活率、受精能力及精子脂质过氧化反应等具有重要的影响和双向调节作用. NO是目前研究最多的体内信息分子和效应分子, 尤其是在生殖系统中的作用已得到广泛而深入的研究, 本文对NO与精子及其获能关系的研究概况作一综述
早泄的遗传病因学研究进展

早泄(premature ejaculation, PE)是最常见的男性性功能障碍之一. 长期以来对其发病原因归结为不良精神心理;环境等因素引起, 与性交时焦虑、恐惧和紧张, 性知识缺乏和误解等有关. 然而, 近来对其神经生物学、内分泌, 以及遗传病因学的研究越来越受重视, 尤其原发性早泄(lifelong PE)的遗传学研究得到极大关注[1-4]. 现就早泄的遗传病因学研究进展作一综述
DEC1 nuclear expression: A marker of differentiation grade in hepatocellular carcinoma

AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis. METHODS: The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues. RESULTS: DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated(83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024). CONCLUSION: DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation
Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene

AIM: To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS: We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family, member 1 (ING1) expression. RESULTS: Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentiation and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion, tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. INGI is a direct target of miR-622. CONCLUSION: These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer
Legalon-SIL downregulates HCV core and NS5A in human hepatocytes expressing full-length HCV

AIM: To determine the effect of Legalon-SIL (LS) on hepatitis C virus (HCV) core and NS5A expression and on heme oxygenase-1 (HMOX-1) and its transcriptional regulators in human hepatoma cells expressing full length HCV genotype lb. METHODS: CON1 cells were treated with 50 μmol/L or 200 μmol/L LS. Cells were harvested after 2, 6 and 24 h. HCV RNA and protein levels were determined by quantitative real-time polymerase chain reaction and Western blotting, respectively. RESULTS: HCV RNA (core and NS5A regions) was decreased after 6 h with LS 200 μmol/L (P < 0.05). Both 50 and 200 μmol/L LS decreased HCV RNA levels [core region (by 55% and 88%, respectively) and NS5A region (by 62% and 87%, respectively) after 24 h compared with vehicle (dimethyl sulphoxide) control (P< 0.01). Similarly HCV core and NS5A protein were decreased (by 85%, P < 0.01 and by 65%, P < 0.05, respectively) by LS 200 μmol/L. Bachl and HMOX-1 RNA were also downregulated by LS treatment (P < 0.01), while Nrf2 protein was increased (P < 0.05). CONCLUSION: Our results demonstrate that treatment with LS downregulates HCV core and NS5A expression in CON1 cells which express full length HCV genotype 1b, and suggests that LS may prove to be a valuable alternative or adjunctive therapy for the treatment of HCV infection
Glutamate reduces experimental intestinal hyperpermeability and facilitates glutamine support of gut integrity

AIM: To assess whether glutamate plays a similar role to glutamine in preserving gut wall integrity. METHODS: The effects of glutamine and glutamate on induced hyperpermeability in intestinal cell lines were studied. Paracellular hyperpermeability was induced in Caco2.BBE and HT-29CL.19A cell lines by adding phorbol-12,13-dibutyrate (PDB) apically, after which the effects of glutamine and glutamate on horseradish peroxidase (HRP) diffusion were studied. An inhibitor of glutamate transport (L-trans-pyrrolidine-2,4-dicarboxylic acid: trans-PDC) and an irreversible blocker (acivicin) of the extracellular glutamine to glutamate converting enzyme, γ-glutamyltransferase, were used. RESULTS: Apical to basolateral HRP flux increased significantly compared to controls not exposed to PDB(n = 30, P < 0.001). Glutamine application reduced hyperpermeability by 19% and 39% in the respective cell lines. Glutamate application reduced hyperpermeability by 30% and 20%, respectively. Incubation of HT29CL.19A cells with acivicin and subsequent PDB and glutamine addition increased permeability levels. Incubation of Caco2.BBE cells with trans-PDC followed by PDB and glutamate addition also resulted in high permeability levels. CONCLUSION: Apical glutamate -similar to glutaminecan decrease induced paracellular hyperpermeability. Extracellular conversion of glutamine to glutamate and subsequent uptake of glutamate could be a pivotal step in the mechanism underlying the protective effect of glutamine
冷冻切片固定液的改进

冷冻切片质量的好坏直接关系到病理诊断的及时性和准确性.本文重点对冷冻制片中固定液的使用及其操作方法进行探讨,力求解决或改进冷冻切片中存在的一些质量问题,以提高快速病理诊断的准确性
儿童骶尾部有尾畸形1例

讨论骶尾部的畸形和肿瘤介于胚胎学及畸变学之间.在胚胎发育阶段可能遗留下某些问题,使骶尾部成为婴幼儿最常发生畸形及肿瘤的部位之一[1].我们所见到的骶尾部畸形有脊柱裂,错构瘤,表皮样及皮样囊肿,淋巴管扩张等.而人类骶尾部有尾畸形少见,但是lumbosacrococcygeal地区突出的先天性异常病变[2],是胚胎发育过程中的异常事件.据国外文献报道,人类胚胎有一个10~12个尾椎的尾巴,在孕7和8周,如尾巴回归失败,则可能出现胚胎残余尾.其次,人类真正的尾巴,或持续退化的尾巴,或源自人类远端残余的胚胎尾巴,它含有脂肪和结缔组织,中央束或肌肉,血管和神经,由皮肤覆盖,而骨,软骨,脊索,脊髓等缺乏[3,4],与神经管等无联系,手术容易切除,无后遗症[3].本例出生时即有尾畸形,随年龄增长至14岁时已长达7 cm,实属罕见;其组织学显示为皮肤包裹的脂肪,肌肉及神经纤维,未见骨骼,软骨及脊髓神经,符合罕见胚胎发育异常之有尾畸形.由此提示,在现代技术水平条件下及时的孕期检查是必不可少的重要环节.当胎儿发育到7~8个月大的时候,通过B超等检查可以发现此类畸形以及与神经系统的关系,可在出生后尽早行尾巴切除术
丙型肝炎病毒与其合并人类免疫缺陷病毒感染者的病毒基因型差异

HCV与HIV具有相似的传播途径, 都可以通过使用污染的血制品、注射用具、性接触及母婴传播, 因此, HIV与 HCV混合感染较常见. HIV感染改变HCV的自然病程, 加速肝纤维化、肝硬化及肝细胞癌的进程已达成共识; HCV基因型对肝病的进程和预后亦起到重要作用. 那么HCV基因型的分布在HIV/HCV混合感染与HCV单纯感染之间是否存在差异?各自的感染途径有何不同?值得我们作进一步的探讨. 我们对昆明市第三人民医院收治的昆明地区85例HCV感染住院患者采用PCR等分子生物学方法研究了HIV/HCV感染与HCV单纯感染患者HCV基因型分布的差异
原发性肝癌规范化病理诊断方案专家共识

我国是肝癌高发国家之一. 随着肝脏外科诊断、治疗技术的不断发展, 我国肝癌临床病理诊断学也得到了快速发展, 在肝癌病理标本的数量和类型上具有明显优势. 为制订个体化治疗方案、提高肝癌远期疗效, 临床上对肝癌病理报告的内容也提出了新的要求. 虽然目前已有医疗单位积累了数千甚至数万例的诊断经验, 但从总体上看, 我国肝癌病理诊断的发展仍不平衡, 病理报告的内容及格式差异很大, 还不能完全满足临床个体化精细治疗的需要. 为此, 由中国抗癌协会肝癌专业委员会、中国抗癌协会临床肿瘤学协作专业委员会、中华医学会肝病学分会肝癌学组和全国肝胆肿瘤及移植病理协作组共同组织的"原发性肝癌规范化病理诊断方案专家共识研讨会"于2010年1月17日在上海举行. 会上, 相关专业学会的主要负责人及与会专家学者就原发性肝癌的规范化病理诊断问题展开了热烈研讨, 并对所在单位对肝脏肿瘤免疫病理和分子病理诊断与研究实践中积累的经验和方法进行了广泛交流, 从临床诊断、治疗的实际需要角度对肝癌的规范化病理诊断提出了许多富有建设性的意见和建议, 并介绍了国外一些医院在肝脏肿瘤专科病理诊断方面的方法, 力求制订出一个能较好满足我国临床需要的肝脏肿瘤病理诊断共识方案. 在此基础上, 提出了初步共识草案, 并进一步扩大征求意见, 反复进行修改, 最终形成该共识方案, 以供临床病理医师在实际工作中参考使用, 并将不断修改完善
氢质子磁共振波谱对轻度认知功能损害的诊断价值

轻度认知功能损害(mild cognitive impairment, MCI)是指有轻度记忆或其他认知功能损害但无痴呆的一组临床综合征,是一种介于健康老年人和轻度痴呆之间的临床过渡状态~[1-2],每年10% - 15%的MCI转变为阿尔茨海默病(AD),是健康老年人发生AD的10倍~[3].我们观察MCI与健康老年人在左侧海马等部位的氢质子磁共振波谱(IH-MRS)表现差异,旨在探讨H-MRS对于MCI的诊断价值及所示代谢物比值与认知损害的相关性
老年支气管哮喘患者IL-18,IL-12水平的变化及临床意义

目的探讨老年支气管哮喘患者外周血白细胞介素18(IL-18)和IL-12的变化及其在哮喘发病机制中的作用.方法采用ELISA法检测42例老年支气管哮喘患者及26例正常对照组检测血清中IL-18及IL-12水平.结果支气管哮喘发作期患者的血浆IL-12明显高于正常对照组(P<0.01),IL-18明显高于正常对照组(P<0.01).结论 IL-18,IL-12参与了老年支气管哮喘发作期的发病机制,IL-18,IL-12在Th1/Th2细胞因子网络失衡的发病机制中起重要的调节作用
人工肱骨头置换治疗肱骨近端Neer III和Ⅳ型骨折

Neer III、IV型骨折, 是一种严重的肱骨近端骨折, 切开复位内固定手术难度大, 且术后肱骨头坏死率高, 在2、3部分骨折, 肱骨头缺血坏死的发生率是3％~14％, 而在4部分骨折, 其发生率高达26％~75％[1], 而且并发症多. 用人工肱骨头假体代替粉碎的肱骨头, 不存在肱骨头的缺血坏死及不愈合的并发症, 对缓解疼痛、增加肩关节的活动度、改善关节功能有确切疗效[2]. 作者自2007年1月~2010年8月, 对15例肱骨近端Neer III、IV型骨折患者, 行人工肱骨头置换, 获得良好疗效, 现报告如下
医院消毒供应集中式管理的实践

医院消毒供应集中式管理,是指对所有需要消毒和灭菌后重复使用的诊疗器械,器具和物品由医院消毒供应中心(CSSD)回收,集中清洗,消毒,灭菌和供应.从2006年起,我院逐步实行医院消毒供应集中管理,收效良好
牙龈炎冲洗器用于重型颅脑损伤患者的口腔护理观察

正常人口腔因进食,饮水,漱口,唾液循环等有自净作用.唾液中的溶菌酶还有杀菌作用,因而很少发生口腔感染,而重型颅脑损伤患者由于昏迷,高热,手术等不能经口进食,口腔内菌群失调,大量细菌繁殖,分解残留在口腔内的食物残渣,产生吲哚,硫氢基,氨类物质,发生口臭,口腔炎症,溃疡,呼吸道感染等.而牙龈炎冲洗器是一种DP300的复方含漱液,它的主要成分是DP300,茶多酚,甘草甜素,黏膜保护剂及渗透剂等.DP300对革兰阳性及阴性菌,真菌,病毒具有广泛的杀菌及抑制作用,特别对引起口腔溃疡,牙龈炎,牙周炎及口臭的病菌具有有效的杀菌作用,对皮肤黏膜无刺激性.茶多酚是一种天然植物抗菌药物,具有广谱,强效,无毒的特点,还有抗氧化性能快速去除口臭的功能.甘草甜素具有抗病毒,提高免疫力,清除自由基及抗溃疡的作用.因此,为了使重型颅脑损伤患者保持口腔清洁,预防口腔感染,口臭和呼吸道感染,增强机体抵抗力,对颅脑损伤患者应用牙龈炎冲洗器中的冲洗液进行口腔护理十分必要
浅析消毒供应中心器械的除锈与保养

经除锈处理过的器械,锈渍明显消失,器械恢复到一定的光泽度,提高了器械的使用率,同时节约了成本.经润滑保养后的器械,轴,关节处的灵活度明显提高,也不易生锈
经腹经骶柱状切除技术治疗复发直肠癌

复发直肠癌再次手术的经典方法一般都采用截石位经腹经会阴途径, 施行再次切除吻合保肛或者后盆腔切除乃至全盆腔切除. 从理论和实践中看, 这个经典途径最大的难点在于直肠与阴道/前列腺的关系不能直视, 而对于复发性直肠癌的再次手术而言, 复发癌与阴道/前列腺关系的处理直接关系到手术类型和手术难度, 例如: (1)是否需要切除一部分阴道/前列腺组织. (2)如何掌握切除部分的范围和厚度. (3)是否真的需要全切前列腺而施行全盆腔切除. (4)手术中手术后如何止血. 另外, 复发直肠癌病人通常都需接受放化疗, 其再次手术后会阴部切口能否愈合也是一个常见问题
重视基于体素的形态测量学在脑结构变化评价中的应用

MRI是研究中枢神经系统疾病脑结构变化的重要工具,传统的MR分析脑结构的方法包括观察法、径线测量法及ROI法,这些方法仅能测量脑区明显的病变、客观性较低、耗时耗力、可重复性差.随着计算机后处理技术的发展及新成像方法的不断涌现,使得活体(in vivo)研究脑结构变化成为可能.基于体素的形态测量学(voxel-based morphometry, VBM)是一种全面、客观、无偏倚的脑结构影像分析技术,可以定量分析脑结构的微小变化,发现隐匿性脑结构的损伤.自1995年Wright等~[1]提出基于体素的脑结构分析的概念后,VBM技术已经成为脑形态测量学的重要技术之一,迄今为止已发表相关的论文1270篇左右,并已广泛应用于临床各种疾病脑结构损伤的研究之中,如心功能不全患者脑灰质的减少、智商与脑灰质体积的相关性研究、帕金森病脑结构的研究、利手的小脑体积不对称性研究、年龄和性别对脑解剖结构的影响、多系统萎缩脑结构的研究以及肌萎缩侧索硬化症脑结构的研究等[2-7].因此,有必要了解并重视VBM技术在脑结构评价中的应用