mlsa-iai-msu-lab/ru_sci_bench_zho_cite_reranking huggingface dataset:
Chlorobenzenes and organochlorinated pesticides in vegetable soils from an industrial site, China

Organochlorinated compounds are ubiquitous contaminants in the environment, especially in industrial sites. The objective of the work was to investigate whether a vegetable field near an industrial site is safe for vegetable production. The residues of chlorobenzenes (CBs), hexachlorocyclohexanes (HCHs) and dichlorodiphenyltrichloroethanes (DDTs) in a vegetable field which was near a chemical plant in China were characterized. Point estimate quotient was used for ecological risk assessment of the investigated site. The results showed that all CBs except monochlorobenzene (MCB) were detected in soils. The total concentrations of ∑ CBs ranged from 71.06 to 716.57 ng/g, with a mean concentration of 434.93 ng/g. The main components of CBs in soil samples were dichlorobenzenes (DCBs), trichlorobenzenes (TCBs) and tetrachlorobenzenes (TeCBs), while for single congeners, 1,2,4-TCB had the highest concentration, which ranged from 13.21 to 210.35 ng/g with a mean concentration of 111.89 ng/g. Residues of hexachlorobenzene (HCB) in soil samples ranged from 0.9 to 11.79 ng/g, significantly lower than ∑ DCB, ∑ TCB and ∑ TeCB. Concentrations of ∑ HCHs and ∑ DDTs in soils ranged from 11.32 to 55.24 ng/g and from 195.63 to 465.58 ng/g, respectively, of which the main components were α-HCH and p,p’-dichlorodiphenyldichloroethylene (p,p’-DDE). Ecological risk assessment for the investigated site showed that the most potential risks were from TCBs and TeCBs, based on the hazard quotients. The higher residues of CBs and DDTs compared to the target values and the higher than 1 hazard quotients indicated that this area is not safe for vegetable production and thus soil remediation is needed.
Spatial distribution and pollution assessment of heavy metals in urban soils from southwest China

To identify the concentrations and sources of heavy metals, and to assess soil environmental quality, 63 soil samples were collected in Yibin City, Sichuan Province, China. Mean concentrations of As, Pb, Zn, and Cu were 10.55, 61.23, 138.88 and 56.35 mg/kg, respectively. As concentrations were comparable to background values, while Pb, Zn, and Cu concentrations were higher than their corresponding background values. Industrial areas exhibited the highest concentrations of As, Pb, Zn, and Cu, while the lowest concentrations occurred in parks. Statistical analysis was performed and two cluster groups of metals were identified with Pb, Zn, and Cu in one group and As in the other. Spatial distribution maps indicated that Pb, Zn, and Cu were mainly controlled by anthropogenic activities, whereas As could be mainly accounted for by soil parent materials. Pollution index values of As, Pb, Zn, and Cu varied in the range of 0.24-1.93, 0.66-7.24, 0.42-4.19, and 0.62-5.25, with mean values of 0.86, 1.98, 1.61, and 1.78, respectively. The integrated pollution index (IPI) values of these metals varied from 0.82 to 3.54, with a mean of 1.6 and more than 90% of soil samples were moderately or highly contaminated with heavy metals. The spatial distribution of IPI showed that newer urban areas displayed relatively lower heavy metal contamination in comparison with older urban areas.
Effects of land use, climate, topography and soil properties on regional soil organic carbon and total nitrogen in the Upstream Watershed of Miyun Reservoir, North China

Soil organic carbon (SOC) and total nitrogen (TN) contents as well as their relationships with site characteristics are of profound importance in assessing current regional, continental and global soil C and N stocks and potentials for C sequestration and N conservation to offset anthropogenic emissions of greenhouse gases. This study investigated contents and distribution of SOC and TN under different land uses, and the quantitative relationships between SOC or TN and site characteristics in the Upstream Watershed of Miyun Reservoir, North China. Overall, both SOC and TN contents in natural secondary forests and grasslands were much higher than in plantations and croplands. Land use alone explained 37.2% and 38.4% of variations in SOC and TN contents, respectively. The optimal models for SOC and TN, achieved by multiple regression analysis combined with principal component analysis (PCA) to remove the multicollinearity among site variables, showed that elevation, slope, soil clay and water contents were the most significant factors controlling SOC and TN contents, jointly explaining 70.3% of SOC and 67.1% of TN contents variability. Only does additional 1.9% and 3% increase in the interpretations of SOC and TN contents variability respectively when land use was added to regressions, probably due to environment factors determine land use. Therefore, environmental variables were more important for SOC and TN variability than land use in the study area, and should be taken into consideration in properly evaluating effects of future land use changes on SOC and TN on a regional scale.
PartialRC: A Partial Recomputing Method for Efficient Fault Recovery on GPGPUs

GPGPUs are increasingly being used to as performance accelerators for HPC (High Performance Computing) applications in CPU/GPU heterogeneous computing systems, including TianHe-1A, the world’s fastest supercomputer in the TOP500 list, built at NUDT (National University of Defense Technology) last year. However, despite their performance advantages, GPGPUs do not provide built-in fault-tolerant mechanisms to offer reliability guarantees required by many HPC applications. By analyzing the SIMT (single-instruction, multiple-thread) characteristics of programs running on GPGPUs, we have developed PartialRC, a new checkpoint-based compiler-directed partial recomputing method, for achieving efficient fault recovery by leveraging the phenomenal computing power of GPGPUs. In this paper, we introduce our PartialRC method that recovers from errors detected in a code region by partially re-computing the region, describe a checkpoint-based faulttolerance framework developed on PartialRC, and discuss an implementation on the CUDA platform. Validation using a range of representative CUDA programs on NVIDIA GPGPUs against FullRC (a traditional full-recomputing Checkpoint-Rollback-Restart fault recovery method for CPUs) shows that PartialRC reduces significantly the fault recovery overheads incurred by FullRC, by 73.5% when errors occur earlier during execution and 74.6% when errors occur later on average. In addition, PartialRC also reduces error detection overheads incurred by FullRC during fault recovery while incurring negligible performance overheads when no fault happens.
肺癌皮肤转移1例

患者男,81岁。因咳嗽2个月余,加重伴胸闷2d,于2009年9月17日收人呼吸科。患者2个月前出现咳嗽,随即发现背部一红色结节伴轻度疼痛,自行用抗生素治疗未见明显好转。患者有前列腺增生病史,目前排尿困难,既往史及家族史无特殊。体格检查:系统检查未见明显异常。皮肤科检查:背部一2.0cm X 3.0 cm红色肿块,表面光滑,质硬,有浸润感,边界不清(图1A),活动度差,轻度压痛。实验室及辅助检查:尿常规中潜血(+++),蛋白(+);血清白蛋白34g/L(正常值35~55g/L,以下同);血沉38mm/1 h(<15 mm/1 h);癌胚抗原8.33 |xg/μL(<5 μg/ L);余未见异常。胸部CT:左肺下叶中心型肺癌伴下叶局限性阻塞性不张;支气管镜检查示:中心型肺癌,同时取材送组织病理检查:中分化鳞状细胞癌。全身骨显像(SPECT)、腹部彩超等检查未见异常。诊断:肺癌、皮肤转移癌,并建议皮损扩大切除加组织病理检查。
局限性血管角化瘤1例

患儿男,12岁。因左小腿紫红色丘疹、疣状斑块伴疼痛12年,于2010年8月11日来我科就诊。患儿自出生时左小腿外侧即有绿豆大紫红色斑丘疹3处,呈线状排列无自觉症状,当时未予治疗,随年龄的增长斑块渐增大,呈疣状簇集状斑块,近期偶感疼痛。父母非近亲婚配,家族中其父有类似疾病,但皮损较轻。体格检査:一般情况较好,浅表淋巴结无增大,系统检查未见异常。皮肤科检查:左小腿外侧3处簇集呈线状排列的不规则紫红色疣状斑块,无溃破,触之较硬,其边界清楚,边缘呈紫红色、上附小片状鳞屑(图1A)。实验室检查:血、尿、粪常规,肝肾功能均正常。皮损组织病理检查:表皮板层状角化过度,多处角质栓形成,乳头瘤样增生,乳头区及真皮浅层可见大量扩张和增生的血管,管腔内可见大量红细胞(图1B、C)。结合临床及皮损组织病理改变诊断为局限性血管角化瘤。
文身肉芽肿反应1例

患者女,34岁。因左外踝上部皮肤文身后出现红肿伴瘙痒5个月余,于2010年8月3日就诊于我院皮肤科门诊。5个月前患者在左外踝上部皮肤处进行文身,文身后在其表面覆一保护膜,并嘱其留置一段时间。但患者在文身后不久即将保护薄揭去,并于户外行走多时。嗣后文身处皮肤的红色部分出现红肿,伴瘙痒,皮损渐增厚,形成斑块,但无脓液。曾在当地医院接受中药和糖皮质激素等对症处理无效(具体药品及剂量不详),遂行组织病理检查,结果显示表皮显著角化过度,颗粒层、棘层肥厚,真皮浅层血管扩张,周围较多淋巴细胞浸润,并可见较多紫红色染料弥散分布。患者为排除皮损是否有真菌感染而就诊于我科。患者曾于1个月前在腰部进行文身未见异常改变。体格检查:各系统检查未见明显异常。皮肤科检查:左外踝上部一约3cmX5cm红色至暗红色肥厚性斑块,与文身形状相同,呈蝴蝶形,境界清楚,表面凹凸不平,无明显破溃及脓性分泌物,蝴蝶绿色胡须部分正常(图1A)。患者腰部文身处未见异常改变,为花朵图案,也包含红色染料。再次行皮损组织病理检查,结果显示表皮角化过度,真皮浅中层大量色素颗粒,淋巴细胞呈灶状浸润’伴少量中性粒细胞及多核巨细胞(图1B、C),PAS染色未见真菌成分。皮损组织真菌镜检和培养均为阴性。临床诊断:文身肉芽肿反应。建议患者在当地医院采用新型脉冲激光治疗。
D-二聚体检测在静脉血栓栓塞性疾病诊治中的应用

静脉血栓栓塞症(VTE)包括深静脉血栓形成(DVT)和肺栓塞(PE),是危害人类健康的常见疾病之一,发病率和病死率高。虽然目前我国尚缺乏精确的流行病学资料,但VTE在发达国家的发病率高达(1 ~2)/1000人年。在美国,每年约30万患者死于PE.VTE还具有高复发率的特点,其5年和10年的累计复发率分别高达25%和30%,被认为是一种慢性复发性疾病,并可导致血栓形成后综合征、慢性肺动脉高压等远期并发症。因此,对于VTE患者进行及时准确地早期诊断和规范化治疗具有重要的意义。D-二聚体是机体凝血和纤溶系统激活后形成的特异性的降解产物。目前 D-二聚体的检测在VTE诊治中的应用价值已得到大量研究的证实和广泛认可,并已逐步推广应用于其他血栓相关性疾病的诊断、病情监测和预后判断。
Childhood leukemia genetic bottleneck phenomenon related to TEL-AML1: the postulation by a mathematical model

Childhood leukemia bottleneck phenomenon is the most mysterious corollary of the prenatal origin discovery of leukemogenic chromosome translocations. The bottleneck is evidence that leukemia initiation, by in utero acquired chromosome translocations that generate functional fusion genes, is far more common than the incidence rate of corresponding leukemia. For childhood TEL-AML1~+ acute lymphoblastic leukemia (ALL) this equates to approximately 100 times. Practically this means that among a hundred children born with TEL-AML1 fusion gene, only one child will later in its life develop ALL. The key data necessary for unraveling of this mystery were discovered in 2002. It was the level of TEL-AML1~+ cells' frequency. The bottleneck is caused by the very low body TEL-AML1~+ cell count. Only one out of a thousand B cells carries TEL-AML1 fusion gene. TEL-AML1~+ body cell count is low because TEL-AML1 fusion is generated at cell level of 10~(-3) to 10~(-4) just during the late fetal lymphopoiesis i.e. after the 36th gestational week.
Defective T wave combined with incomplete right bundle branch block: a new electrocardiographic index for diagnosing atrial septal defect

Background Incomplete right bundle branch block (ICRBBB) is commonly associated with atrial septal defect (ASD), but lacks sufficient diagnostic test characteristics. An abnormal T wave is also often observed in ASD, with horizontal or inverted displacement of the proximal T wave limb in the right precordial leads, termed "defective T wave" (DTW). Methods We examined the diagnostic test characteristics of combining ICRBBB with DTW as a new index to diagnose ASD. A total of 132 consecutive patients with ASD and 132 cases of age/gender-matched controls without ASD were enrolled. Results Sensitivities of DTW, ICRBBB, and both were 87.1%-87.9%. Specificities were 97.0%, 96.2%, and 100%, respectively. Positive predictive values were 1.3%, 1.1%, and 100.0% respectively, while negative predictive values were 99.9% for each. Conclusion Combining ICRBBB with DTW in electrocardiogram (ECG) as a new index significantly increased the specificity and positive predictive values while maintaining a high sensitivity in diagnosing ASD.
Clinical outcomes after recanalization of a chronic total occluded vessel with bifurcation lesions: results from single-center, prospective, chronic total occlusion registry study

Background Stenting strategies and clinical outcomes of bifurcation lesions in a chronic total occlusion (CTO) vessel after successful recanalization remain to be unknown. Methods Between January 2001 and December 2009, 195 (41.1%) patients with 254 (47.0%) bifurcation lesions in CTO vessels from a pool of 564 patients with 659 CTO lesions were included and divided into proximal (n=134) and distal (n=120) groups, according to the location of the bifurcation lesions. The primary endpoint was the occurrence of major adverse cardiac events (MACE) at the end of clinical follow-up, including cardiac death, myocardial infarction, or target vessel revascularization (TVR). Results Collaterals with Rentrop class 3 were seen more in distal group (100% and 68.3%), compared to proximal group (76.9% and 45.6%). Two-stent technique for proximal bifurcation lesions was used in 24.6%, significantly different from the distal group (6.7%, P <0.001), without significant difference in composite MACE between proximal and distal groups, or between one- and two-stent subgroups in proximal group. The composite MACE after 1-year in complete revascularization subgroup was 17.9% relative to 29.6% in the incomplete revascularization group (P=0.044). Stents in long false lumen in main vessel were mainly attributive to decreased TIMI grade flow, with resultant increased in-stent restenosis, total occlusion, TVR and coronary aneurysms. Imcomplete revasculzarization (HR 2.028, P=0.049, 95% CI 1.002-4.105) and post-stenting TIMI flow (HR 6.122, P=0.020, 95% CI 1.334-28.092) were two independent predictors of composite MACE at the 1-year follow-up. Conclusions Two-stent was more used for proximal bifurcation lesions. No significant difference was observed in MACE between proximal and distal, or between one- and two-stent subgroups in the proximal group. Placement of a safety wire was critical for proximal bifurcation lesions. Complete revascularization was mandatory to improve clinical outcomes.
不同电刺激方式促进脑卒中后功能恢复的机制研究进展

电刺激对脑损伤后功能恢复具有显著的促进作用,但目前关于电刺激促进功能恢复的机制研究尚不足以说明其作用的确切机制。不论是从损伤脑组织结构和功能重组、内源性神经干细胞增殖分化还是抗凋亡通路等方面研究电剌激的作用机制,都需要进一步的深人研究。
Density Functional Study of Cationic and Anionic AgmCun (m+ n ≤ 5) Clusters

The structural and energetic properties of bimetallic AgmCun clusters (m + n ≤ 5) in the cationic and anionic charged states have been investigated by density functional theory with relativistic effective core potentails. The stable cationic pentamers have three-dimensional structures in contrast to anionic clusters assume planar structures. For the given cluster size the electron affinities decrease as the Cu content increases, while no clear trend can be found in adiabatic ionization potentials. The binding energy per atom also increases with the increasing Cu content and follows the order anion < cation. The most probable dissociation channels of the clusters considered are also discussed.
Ilizarov技术治疗感染后小腿短缩畸形一例报告

讨论化脓性骨髓炎常见的并发症包括关节强直、关节畸形、患胺增长或短缩、病理性骨折,甚至癌变等~([1])。患肢发育畸形是因炎症刺激骨骺而导致骺板过度生长或生长障碍,最终严重影响肢体功能。感染后骨不愈合及骨缺损是矫形外科治疗中的难点,多需借助外固定技术进行治疗~([2]).Ilizarov技术是治疗重度僵硬性肢体畸形和大段骨缺损的有效方法~([3-5])。本例患者为3岁时发生左小腿急性化脓性感染,感染控制后未曾进一步手术治疗,遗留左胫骨骨质破坏及发育不良。此畸形特点为胫骨大段骨缺损、腓骨内翻、反屈畸形,左胫、腓骨远端融合,严重影响左膝、左踝运动及下肢负重功能。我们尝试应用Ilizarov技术结合有限矫形一期矫正复合畸形,创伤小,未出现特殊并发症,取得了满意的临床效果。
牵拉成骨技术治疗慢性骨髓炎

慢性骨髓炎的治疗是骨科临床的疑难问题,其局部感染会形成抑制血液循环和骨再生的局部环境,抑制或延缓骨的愈合~([1])。由于该病几年甚至几十年不能治愈,所以患者精神受到极大摧残,严重者甚至会选择截肢~([2])。传统的治疗慢性骨髓炎的方法是彻底清除死骨及炎性肉芽组织,消灭死腔及窦道,充分引流,控制感染,待局部皮肤条件好转后植骨,并选择合适的外固定方式固定。但这种方法治疗周期长、创伤较大、手术次数多,容易发生严重骨缺损、骨不愈合、畸形愈合等并发症~([3])。近年来,随着Ilizarov牵拉成骨(distraction osteogenesis, DO)技术在国内的逐渐推广,许多学者将DO技术应用于治疗慢性骨髓炎,尤其是伴有骨与软组织缺损或合并骨骼畸形的慢性骨髓炎,并取得了令人惊喜的效果~([4-7])。本文就DO技术治疗慢性骨髓炎的机制、临床应用中的技术要点等进行综述。
Hyperedge Communities and Modularity Reveal Structure for Documents

Words and their association in documents could be represented by hypergraph in a standard way. Communities of words often overlap. Accepting a community should have more internal than external connections, we view every hyperedge as a vertex, establish a network for hyperedges by their similarity, and use the method of modularity to find their communities. The example here shows that email address' communities, generated by pulling back hyperedges communities, naturally incorporate overlap and reveal hierarchical organization.
Adsorption, Diffusion, and Dissociation of H_2O on Kaolinite (001): a Density Functional Study

Density functional theory is used to investigate the adsorption, diffusion, and dissociation of H_2 O on kaolinite(001) surface. It is found that the preferred adsorption sites on the kaolinite(001) surface for H_2 O are the threefold hollow sites with the adsorption energies ranging from 1.06 to 1.15 eV. H_2 O does not adsorb on the six-fold hollow site of the aluminium(001) face of the third layer of kaolinite, implying that it is difficult for water molecules to penetrate the ideal kaolinite(001) surface. In addition, we calculate the energetic barriers for the diffusion of H_2 O between the most stable and next most stable adsorption sites, which range from 0.073 to 0.129eV. The results also show that H_2 O molecules are easy to diffuse on kaolinite(001) surface. Finally, our study indicates that no dissociation state exists for the H_2 O on kaolinite(001) surface.
A Single-Transistor Active Pixel CMOS Image Sensor Architecture

A single-transistor CMOS active pixel image sensor (1 T CMOS APS) architecture is proposed. By switching the photosensing pinned diode, resetting and selecting can be achieved by diode pull-up and capacitive coupling pull-down of the source follower. Thus, the reset and selected transistors can be removed. In addition, the reset and selected signal lines can be shared to reduce the metal signal line, leading to a very high fill factor. The pixel design and operation principles are discussed in detail. The functionality of the proposed 1T CMOS APS architecture has been experimentally verified using a fabricated chip in a standard 0.35 μm CMOS AMIS technology.
Polarization Beam Splitter Based on an Annular Photonic Crystal of Negative Refraction

An annular photonic crystal is used to design a novel kind of polarization beam splitter based on the negative refraction effect. Since TE and TM polarization waves can be excited at the first and second bands, respectively, they will result in different refractive angles and make separation possible. To improve the splitting efficiency, the cut-off treatment along the Γ-M direction and the antireflection coating are introduced to enlarge the separation angle and to decrease the reflection loss. Simulation results show that the proposed polarization beam splitter has a tunable working incident angle that is in the range of 18°–26°. In particular, when the incident angle is 20°, over 90% transmissions can be achieved for both TE and TM polarizations in a wide working frequency range from 0.279(2πc/a) to 0.287(2πc/a).
Chlorocholine chloride and paclobutrazol treatments promote carbohydrate accumulation in bulbs of Lilium Oriental hybrids 'Sorbonne'

The present study was to test the hypothesis that the plant growth retardants chlorocholine chloride(CCC) and paclobutrazol(PBZ)could improve the carbohydrate accumulation in lily bulbs by enhancing photosynthetic capacity and changing endogenous hormones.Plants of Lilium Oriental hybrids’Sorbonne’were treated with a foliar spray of CCC or PBZ(both at 300 mg/L)solution,at six weeks after planting(6 WAP).The morphological parameters,endogenous hormone contents(gibberellic acid(GA),abscisic acid(ABA),and indole-3-acetic acid(IAA)),and carbohydrate contents were measured from 6 to 18 WAP,at 2-week intervals.The results showed that CCC increased the biomass of leaves and stems which might produce more photoassimilates available for transportation and utilization.However,PBZ treatment suppressed vegetative growth and favored photoassimilate transportation into bulbs.A slight delay of bud and anthesis formation was observed in both treated plants.CCC and PBZ treatments substantially enhanced the sucrose contents in leaves probably due to the increase of chlorophyll contents.Treatment with CCC or PBZ decreased GA but increased IAA contents in lily bulbs which might stimulate starch accumulation and formation of new scales.Our experiment suggested that CCC or PBZ treatment is an effective method to promote carbohydrate accumulation in lily bulbs.
Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus(BmNPV)

In the late phase of Bombyx mori nucleopolyhedrovirus(BmNPV)infection,a large amount of polyhedra appear in the infected cell nucleolus,these polyhedra being dense protein crystals protecting the incorporated virions from the harsh environment.To investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV,two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus(polh~+)Bac-to-Bac system,designated as vBmBac(polh~+)-enhanced green fluorescent protein(EGFP)and vBmBac(polh~+)-LacZ,which can express the polyhedrin and foreign protein simultaneously.Light microscopy analysis showed that all viruses produced polyhedra of normal appearance.Green fluorescence can be apparently detected on the surface of the vBmBac(polh~+)-EGFP polyhedra,but not the BmNPV polyhedra.Fluorescence analysis and anti-desiccation testing confirmed that EGFP was embedded in the polyhedra.As expected,the vBmBac(polh~+)-LacZ polyhedra contained an amount of LacZ and had a higherβ-galactosidase activity.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blotting were also performed to verify if the foreign proteins were immobilized into polyhedra.This study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.
合成洗涤剂对玉米的毒性及氧化损伤作用

综上所述，通过洗涤剂对玉米生长、脂质过氧化产物MDA含量及抗氧化酶系影响的研究，发现环境浓度下的洗涤剂会对玉米造成危害，抑制玉米生长，使植物体内活性氧自由基含量增加，破坏植物体内氧化态物质的平衡状态，使植物的膜脂发生过氧化反应，对植物的膜脂造成损伤，破坏各种生物膜的完整性。因此，含洗涤剂的污水用于农田灌溉时应考虑其对农作物的毒性作用，今后应关注含洗涤剂污水的排放及二次使用监管。
对《中国药典》2010年版毒性中药品种的探讨

目的对2010年版《中国药典》(以下简称《药典》)Ⅰ部的毒性中药品种进行探讨,为合理规范使用毒性中药提供依据。方法参考2010版《药典》及对近年来国内外期刊中有关毒性中药研究的文献,进行了检索、分析和总结。结果列出了《药典》2010年版所载毒性中药,并按照毒性大小进行了分类,探讨了毒性中药的用法用量及配伍禁忌。结论掌握毒性中药的毒性大小、分类,用法用量等,对于防止毒性药品的不良反应,杜绝毒药的滥用,维护人类健康具有非常重要的意义。
接骨木抗炎活性部位及活性成分的初步研究

目的用大鼠足肿胀模型作为活性追踪指标,对接骨木枝和叶的60%的乙醇提取物进行抗炎活性部位和成分的追踪鉴定;考察接骨木枝和叶的抗炎作用。方法采用10%鸡蛋清所致的大鼠足肿胀法观察接骨木枝和叶的抗炎作用;运用质谱法(MS)对活性部位进行化合物的结构鉴定。结果接骨木叶的正丁醇萃取物的高、中剂量组在致炎后1h、3 h和6h足肿胀率均处于较低水平,有显著性差异(与空白组比较P<0.05);经串联质谱(MS~n)测定活性部位主要为槲皮素和山萘酚等化合物。结论接骨木叶具有抗炎作用,活性成分主要为槲皮素和山萘酚等黄酮类物质。
皂荚皂苷的提取及其抑菌作用的研究

目的优选水浸提皂荚皂苷的最佳提取工艺以及皂荚皂苷对5种细菌和4种真菌生长的抑制作用。方法水浸提后异丙醇萃取纯化法提纯皂荚皂苷,利用正交设计法确定料水比、提取时间、提取温度的最佳提取工艺条件;采用双倍营养法研究皂荚皂苷的抑菌作用。结果水浸提皂荚皂苷的最佳工艺条件为:温度80℃,时间2 h,料水比1∶10,浸提次数3次,最大提取率31%;皂荚皂苷水溶液对大肠杆菌、金黄色葡萄球菌、绿脓杆菌、阴沟肠杆菌、沙门肠杆菌及白色念珠菌、克柔念珠菌、热带念珠菌、近平滑念珠菌均有抑菌作用,对细菌的抑制效果较好。50 mg/ml的皂荚皂苷溶液能完全抑制5种细菌的生长,对4种真菌的抑制率也均在50%以上。结论水提皂苷是一种方便经济的方法;皂荚皂苷可用于抑菌药物的开发。
退变髓核细胞与正常髓核细胞体外培养的生物学特性对比

目的:比较体外培养的退变髓核细胞与正常髓核细胞生物学特性的差异。方法:将成年新西兰大白兔以纤维环穿刺法椎间盘造模法处理,4周后取退变以及正常髓核细胞,进行细胞形态及电镜观察、MT法试验,检测细胞周期及主要细胞外基质相关基因表达情况。结果:细胞生长曲线显示,传4代细胞第5天开始出现生长减慢,传5~7代细胞均在接种后第2~3天细胞数量明显减少(P<0.05);退变髓核细胞传3代细胞第4天开始出现生长减慢(P<0.05),传5代时即出现生长滞缓,传7代时出现生长停滞。髓核软骨样细胞MTT摄取实验,传4~7代细胞的吸光度值明显低于传1代(P<0.05)。正常和退变髓核细胞细胞所处生长分裂期对比中,正常和退变髓核细胞G1期细胞所占比例具有统计学差异(P<0.05)。结论:大白兔正常髓核细胞持续增殖能力、蛋白聚糖和Ⅱ型胶原DNA的含量均较退变髓核细胞高,能为椎间盘组织工程学、细胞生物学疗法等提供理论基础。
食管鳞癌患者肿瘤微环境中Th17细胞的表达及临床意义

目的:了解Th17细胞在食管鳞癌患者肿瘤微环境中表达水平,探讨其在食管鳞癌进展中的作用。方法:收集健康人外周血、食管鳞癌患者外周血、癌旁组织、癌组织标本,流式细胞术检测Th17细胞水平;实时荧光定量聚合酶链反应(QRT-PCR)检测RORγt、IL-17a mRNA表达水平。结果:食管鳞癌患者外周血、癌旁组织、癌组织中Th17细胞水平和RORγt、IL-17a mRNA表达水平较健康人外周血均升高,且癌组织>癌旁组织>患者外周血>健康人外周血,差异有统计学意义(均P<0.05)。食管鳞癌患者外周血中Th17细胞水平升高与患者性别、年龄、肿瘤大小无明显关系,但与有无淋巴结转移和TNM分期有关(P<0.01)。结论:食管鳞癌患者肿瘤微环境中Th17细胞水平和RORγt、IL-17a mRNA表达水平均升高,Th17细胞可能通过RORγt和IL-17a参与食管鳞癌进展过程。
免疫组化标记前脱黑色素的方法和体会

在免疫组化工作中,经常遇到一些含有黑色素的肿瘤,需要作免疫组化标记,但这些肿瘤细胞中含有大量黑色素沉积,遮盖了正常细胞形态和组织学结构,并和免疫组化阳性结果所显示的棕黄色颗粒互相干扰,以至影响判断。因此,染色前必须行脱黑色素处理。传统方法至少耗时2 h以上,切片在高锰酸钾溶液中长时间浸泡,一方面对组织内其他成分的氧化作用,易造成免疫组化标记的假阴性;另一方面,在此之后的免疫组化过程中,极易造成脱片。如何操作才能既脱了黑色素,又保证免疫组化的效果呢?根据我们长期的摸索,体会报告如下。
白细胞介素-7/白细胞介素-7受体信号途径与类风湿关节炎治疗的研究进展

白细胞介素(interieukin,IL)-7发现于1980年，具有多种生物学功能。IL-7不仅可以刺激T细胞增殖、分化，维持T细胞的存活，而且能诱导单核细胞和B细胞的活化以及破骨细胞的形成。类风湿关节炎(rheumatoid arthritis, RA)患者的滑液和滑膜组织髙表达IL-7，高水平的IL-7参与了 RA的发病。 IL-7通过IL-7受体(IL-7 receptor, IL-7R)介导其在关节炎中的炎症作用。最近研究发现在RA患者免疫细胞表面，IL-7R表达显著增加，阻断IL-7R可以明显减轻关节炎的严重程度，并减少相关促炎症因子的产生，所以通过阻断IL-7R而阻断IL-7的作用可能成为治疗RA的一个有效靶点。
Simulation of high velocity compaction of powder in a two dimension mould using lattice Boltzmann method

This work presents a numerical study on the dynamic high velocity compaction of the metal powder. The analysis of the process is based on a mesoscopic approach using multi-speed lattice Boltzmann method. The boundary condition and the relaxation time are tailored to the situation. The dynamic compaction process is vividly presented and the shock wave can be easily found in the simulation. The density is analyzed in order to explore the mechanism of the high velocity compaction.