# Bioconversion of Sugarcane Biomass into Ethanol: An Overview about Composition, Pretreatment Methods, Detoxification of Hydrolysates, Enzymatic Saccharification, and Ethanol Fermentation

Authors: Larissa Canilha; Anuj Kumar Chandel; Thais Suzane dos Santos Milessi; Felipe Antônio Fernandes Antunes; Wagner Luiz da Costa Freitas; Maria das Graças Almeida Felipe; Silvio Silvério da Silva
Journal: Journal of Biomedicine and Biotechnology
 (2012)

---

## Abstract

Depleted supplies of fossil fuel, regular price hikes of gasoline, and environmental damage have necessitated the search for economic and eco-benign alternative of gasoline. Ethanol is produced from food/feed-based substrates (grains, sugars, and molasses), and its application as an energy source does not seem fit for long term due to the increasing fuel, food, feed, and other needs. These concerns have enforced to explore the alternative means of cost competitive and sustainable supply of biofuel. Sugarcane residues, sugarcane bagasse (SB), and straw (SS) could be the ideal feedstock for the second-generation (2G) ethanol production. These raw materials are rich in carbohydrates and renewable and do not compete with food/feed demands. However, the efficient bioconversion of SB/SS (efficient pretreatment technology, depolymerization of cellulose, and fermentation of released sugars) remains challenging to commercialize the cellulosic ethanol. Among the technological challenges, robust pretreatment and development of efficient bioconversion process (implicating suitable ethanol producing strains converting pentose and hexose sugars) have a key role to play. This paper aims to review the compositional profile of SB and SS, pretreatment methods of cane biomass, detoxification methods for the purification of hydrolysates, enzymatic hydrolysis, and the fermentation of released sugars for ethanol production.


---

## Body

## 1. Introduction

Brazil is the biggest producer of sugarcane in the world. In the 2012/13 harvest, for example, it was estimated that more than 602 million tons of sugarcane will be processed by the Brazilian sugar-alcohol mills. The sugarcane is basically consisted of stem and straw. The sugarcane straw (or trash) is divided in three principal components, that is, fresh leaves, dry leaves, and tops. The sugarcane stem are milled to obtain the cane juice, which is subsequent used for sugar (sucrose) or alcohol (ethanol) production. The residual fraction from the sugarcane stem milling is named bagasse. Sugarcane bagasse (SB) and straw (SS) are normally burned in industries to supply all the energy required in the process. If, instead, both were used for ethanol production, much more ethanol would be produced from each hectare of sugarcane processed.SB and SS are chemically composed of cellulose, hemicellulose and lignin. Cellulose, and hemicellulose fractions are composed of mixture of carbohydrates polymers. A number of different strategies have been envisioned to convert the polysaccharides into fermentable sugars. One of them, the hemicellulose fraction can be hydrolyzed with dilute acids followed by cellulose hydrolysis with enzymes. The cellulosic fraction is solid rich in glucose, and hemicellulosic fraction is liquid rich in xylose, glucose, and arabinose, where both (solid and liquid) can be fermented to produce ethanol.In general, the biological process from converting the lignocellulose biomass to fuel ethanol involves: (1) pretreatment either to remove lignin or hemicellulose to liberate cellulose; (2) depolymerization of carbohydrate polymers to produce free sugars by cellulase mediated action; (3) fermentation of hexose and/or pentose sugars to produce ethanol; (4) distillation of ethanol. Ethanol produced from sugarcane residues is one of the most suitable alternatives for partial replacements of fossil fuels because it provides energy that is renewable and less carbon intensive than gasoline. Bioethanol reduces air pollution and also contributes to mitigate climate change by reducing greenhouse gas emissions.This paper reviews the important information on the structure and chemical composition of sugarcane biomass (SB and SS), pretreatment of biomass, enzymatic hydrolysis of cellulose, conditioning and detoxification of hemicellulosic hydrolysate, bioconversion of sugars into ethanol, and distillation of ethanol (Figure1).Figure 1
Procedural flow diagram for the bioconversion of cane biomass into 2G ethanol.


## 2. Sugarcane versus Other Feedstock for the Ethanol Production

Sugarcane is the main agricultural crop cultivated in Brazil followed by soybean and corn (Table1). Among the feasible raw materials for ethanol production, sugarcane shows the most promising results because it has a high planted area in the Brazil territory and presents the higher quantity of biomass generated which could be eventually converted into ethanol. The costs of ethanol production are directly related with the costs of feedstock that represents more than one-third of the production costs. Furthermore, the costs of feedstock may vary considerably, depending of its geographic locations, availability, and price [1, 2].Table 1
Different feedstock cultivated in the Brazilian territory.
Biomass
Planted area(1000 hectare)
Production of biomass (1000 t)
Wheat
2,166.2
5,788.6
Rice
2,427.1
11,600.3
Sorghum
785.1
2,204.9
Cassava
1,787.5
24,524.3
Soybean
25,042.2
66,383.0
Castor bean
129.6
25.8
Corn
7,596.3
38,861.8
Sugarcane
8,527.8
602,178.8
Barley
88.4
305.1
Source: Conab [3, 4] and Embrapa [5].According to Conab [3], the Brazilian sugar-alcohol mills will process more than 602 million tons of sugarcane in the 2012/13 harvest, leading the production of roughly 39 million tons of sugar and 24 billion liters of ethanol. Each ton of sugarcane processed by the mills generates approximately 270–280 kg of bagasse [6] and 140 kg of straw [7]; thus, it can be inferred that Brazilian mills will produce around 163–169 million tons of sugarcane bagasse and 84 million tons of straw only in the 2012/13 harvest.Nowadays, ethanol producing units employ sugarcane efficiently (first generation). However, it is anticipated that in the coming years, SB along with SS will also be used for 2G ethanol production. The deployment of the SB and SS for ethanol production is favored in Brazil because the production process can be annexed to the sugar/ethanol units already in place, requiring lower investments, infrastructure, logistics, and energy supply [1]. In such a scenario, more ethanol would be produced from the same amount of sugarcane processed, without increasing the area used to sugarcane cultivation [8]. The yield of ethanol is equivalent to 6,000 L/hectare planted. It is estimated that ethanol production could reach 10,000 L/hectare if only half of the SB generated is harnessed for the production of biofuel [1].


## 3. Sugarcane Structure

Sugarcane is any of 6 to 37 species (depending on taxonomic interpretation) of tall perennial grasses of the genusSaccharum (family Poaceae, tribe Andropogoneae). Sugarcane is native of warm temperate climate, common in the tropical regions as Brazil, India, Africa, and Asia pacific. The sugarcane plant morphology can be seen in Figure 2.Figure 2
The sugarcane plant morphology. Adapted from [9].Sugarcane is composed by stem and straw (or trash). Sugarcane stem is the material removed before the milling of cane to obtain a juice which is subsequently used for sugar (sucrose) or alcohol (ethanol) production. SB is the residue from stems after extraction of juice. SS (or trash) is composed by fresh leaves, dry leaves, and tops available before harvesting. Fresh leaves are green and yellow in color, tops are the part of cane plant between the top end and the last stalk node, and dry leaves are normally in brownish color [10]. Potential uses of the leaves include: (1) as a fuel for direct combustion; (2) as a raw material for conversion by pyrolysis to char, oil, and/or gas; (3) as a raw material for conversion by gasification and synthesis to methanol. Potential uses for the tops include: (1) as a ruminant feed, either fresh or dried; (2) as a substrate for anaerobic fermentation to methane; (3) after reduction in water content, for the energy uses listed for cane trash [10, 11]. SB and SS are normally burnt in the open agricultural field after the harvesting of the crop, or in some cases, used as an untapped source of simple sugars that can be utilized for the alcohol production [12].Characterizing the sugarcane stalk, SS and SB, following observations can be interpreted: the sucrose accumulation is greater at the base of the stem, and the amount of reducing sugars and cellulose contents is superior in the tops; the length of the stem depends on some factors like the variety of the plant and the cultural management given, so that an adult stem may have from less than two meters to over four in size, affecting the length and the number of internodes; the diameter of the stem also varies, oscillating in its middle part from 250 to 350 m; the color depends on the chlorophyll content and that of anthocyanins as well as on aspects of agronomy; there is a large variation in the moisture content of the sugarcane material, varying from 13.5% (in dry leaves) up to 82.3% (in the tops); the values of ash, fixed carbon, and volatile matter have little variation among the three components of the straw, with a lower amount of ash for the bagasse; all material present practically the same composition in carbon (~45%), hydrogen (~6%), nitrogen (0.5–1%), oxygen (~43%), and sulfur (~0.1%); mineral composition for alkalis and phosphorus shows some variation among the three components of the SS, indicating that its content grows from the dry leaves to the tops, and is quite higher than SB [10, 11].


## 4. Physical and Chemical Compositions of Sugarcane

Physically, sugarcane is constituted by four major fractions, whose relative magnitude depends on the sugar agroindustrial process: fiber, nonsoluble solids, soluble solids, and water (Figure3). The fiber is composed of the whole organic solid fraction, originally found in the cane’s stem, and characterized by its marked heterogeneity. The nonsoluble solids, or the fraction that cannot be dissolved in water, are constituted mainly by inorganic substances (rocks, soil, and extraneous materials), and it is greatly influenced by the conditions of the agricultural cane processing, types of cutting, and harvesting. Soluble solids fraction that can be dissolved in water are composed primarily of sucrose as well as other chemical components such as waxes, in a smaller proportion [11]. SB or SS which are the focus of 2G ethanol production are lignocellulosic materials chemically composed by cellulose, hemicelluloses, and lignin.Figure 3
General composition of Sugarcane. Adapted from [13].Cellulose is a linear polymer of glucose units linked byβ (1→4)-glycosidic bonds, forming cellobiose that is repeated several times in its chain. This cellulosic fraction can be converted into glucose by enzymatic hydrolysis, using cellulases, or by chemical way, using acids like sulfuric acid, that subsequently can be fermented to ethanol [14, 15]. Hemicellulose is a heteropolysaccharide composed by hexoses (D-glucose, D-galactose, and D-mannose), pentoses (D-xylose, L-arabinose), acetic acid, D-glucuronic acid, and 4-O-methyl-D-glucuronic acid units. The hemicelluloses are classified basically according to the sugars that are present in the main chain of polymer: xylan, glucomannan, and galactan [16]. The hemicellulose differs substantially of cellulose to be amorphous, which make it easier to be hydrolyzed than cellulose [17]. The hemicellulosic fraction can be removed from lignocellulosic materials by some type of pretreatment, like acid or hydrothermal hydrolysis, and liberating sugars, mainly xylose, that subsequently can also be fermented to ethanol [18, 19].Lignin is a complex aromatic macromolecule formed by radical polymerization of three phenyl-propane alcohols, namelyp-coumarilic, coniferilic, and synapilic. In the plant cell wall, lignin and hemicelluloses involve the cellulose elementary fibrils, providing protection against chemical and/or biological degradation [20]. The content of lignin and its distribution are the responsible factors for the recalcitrance of lignocellulosic materials to enzymatic hydrolysis, limiting the accessibility of enzyme, and therefore, the process of delignification can improve the conversion rates of enzymatic hydrolysis [21]. The lignin is primarily used as a fuel, but it can be chemically modified to be used as chelating agent [22], for removal of heavy metals from wastewater [23], or as precursor material for production of add-value products as activated carbon [24], surfactants [25], and adhesives [26].SB of the Brazilian territory is quantitatively composed by 38.4–45.5% cellulose, 22.7–27.0% hemicellulose, and 19.1–32.4% lignin (Table2). Nonstructural components of biomass, namely, ashes (1.0–2.8%) and extractives (4.6–9.1%) are the other substances that compose the chemical compositional of bagasse.Table 2
Chemical composition (% w/w, dry basis) of Brazilian SB reported in the literature.
Component (%)
Reference
Pitarelo [27]1*
da Silva et al. [28]2
Canilha et al. [19]3*
Rocha et al. [15]4*
Brienzo et al. [29]5
Rabelo et al. [30]6
Cellulose
41.1
38.8
45.0
45.5
42.4
38.4
Hemicellulose
22.7
26.0
25.8
27.0
25.2
23.2
Lignin
31.4
32.4#
19.1
21.1
19.6
25.0
Ash
2.4
2.8
1.0
2.2
1.6
1.5
Extractives
6.8
—
9.1
4.6
—
—
Others
—
—
—
—
—
—
*Extractives-free basis.
#Lignin and others.
Extracting solvents:1dichloromethane, ethanol : toluene (1 : 2), ethanol, and hot water; 2none; 3water and ethanol; 4ethanol; 5ethanol; 6none.The ash content of SB is lower than the other crop residues like rice straw and wheat straw (with approximately 17.5 and 11.0% of this compound, resp.). SB is also considered a rich solar energy reservoir due to its high yields and annual regeneration capacity (about 80 t/ha) in comparison with agricultural residues like wheat, grasses, and trees (1, 2, and 20 t/ha, resp.) [31]. The bagasse can also be used as a raw material for cultivation of microorganisms and for bioconversion process for the production of industrial enzymes, xylitol, and ethanol production. Due to these advantages the bagasse is considered not only a subproduct of sugar industry, but also a coproduct of high added value [31].The fact that chemical composition varies for the same type of material did not cause surprise because the major fractions of lignocellulosic materials depend on many factors including plant genetics, growth environment, and processing conditions as well as methods employed for the compositional analysis [32]. It is impossible to compare the composition of samples of different origins, performed by different laboratories that do not use the same methods.The large variation in the values of chemical components also is observed for the SS. Generally it is composed of 33.3–36.1% cellulose, 18.4–28.9% hemicellulose, and 25.8–40.7% lignin (Table3). Ashes (2.1–11.7%) and extractives (5.3–11.5%) are also present in SS. The amount of straw from sugarcane harvesting depends on several factors such as: harvesting system, topping, height, cane variety, age of crop (stage of cut), climate, soil, and others. For example, when mechanically harvested, and depending on the harvesting technology applied, the range of straw that is collected and transported to the mill together with the stems is from 24% to 95% of the total trash available [33].Table 3
Chemical composition (% w/w, dry basis) of Brazilian SS reported in the literature.
Component (%)
Reference
Moriya [34]1*
Pitarelo [27]2*
Saad et al. [35]3*
da Silva et al. [28]4
Luz et al. [36]5
Costa et al. [37]6
Cellulose
36.1
34.4
36.1
33.6
33.3
33.5
Hemicellulose
28.3
18.4
26.9
28.9
27.4
27.1
Lignin
26.2
40.7
26.2
31.8#
26.1
25.8
Ash
2.1
11.7
2.1
5.7
2.6
2.5
Extractives
5.3
11.5
5.3
—
—
—
Others
—
—
—
—
10.6
—
*Extractives-free basis.
#Lignin and others.
Extracting solvents:1ethanol; 2dichloromethane, ethanol : toluene (1 : 2), ethanol, and hot water; 3water; 4none; 5none; 6none.


## 5. Ethanol Production from Sugarcane Biomass

Ethanol production from lignocellulosic biomass (second generation) includes pretreatment of biomass, enzymatic hydrolysis of cellulose, fermentation of hexose/pentose sugars, and recovery of ethanol. Intensive efforts have been made in recent years to develop efficient technologies for the pretreatment of SB, developments of enzymes for enhanced cellulose/hemicellulose saccharification, and suitable technologies for the fermentation of both C6 and C5 sugars [1].


### 5.1. Pretreatment of Sugarcane Biomass

Ideally, the pretreatment of lignocellulosic biomass should (1) increase the accessible surface area and decrystallize cellulose, (2) depolymerize partially cellulose, (3) solubilize hemicellulose and/or lignin, (4) modify the lignin structure, (5) maximize the enzymatic digestibility of the pretreated material, (6) minimize the loss of sugars, and (7) minimize capital and operating costs [38, 39].Figure4 presents scanning electronic microscopy (SEM) of SB before diluted sulfuric acid pretreatment and of cellulignin obtained after pretreatment. A rupture of cellulose-hemicellulose-lignin strong matrix occurred after the pretreatment. In the Figure 4(a), an ordered structure of matrix can be seen, while Figure 4(b) presents a disordered structure of cellulose-lignin complex. It is also possible to find empty spaces between the fibers, as consequence of removal of hemicelluloses and low-crystallinity cellulose flocks [15]. In general, hydrolysate originated after diluted acid pretreatment is rich in the hemicellulose fraction.SEM of natural SB (a) and cellulignin obtained after dilute sulfuric acid pretreatment (b) [40].
(a)
(b)Various pretreatment technologies (alone or in combination) have been proposed in the literature. Broadly, pretreatment technologies can be categorized into 4 types: physical (mechanical); physicochemical; chemical; biological pretreatments. Mechanical pretreatment increases the surface area by reducing the size the SB or SS [41]. A high control of operation conditions is required in the physicochemical methods because these reactions occur at high temperature and pressure [21]. Chemical methods degrade hemicellulose or remove lignin and thus, loosening the structural of lignin-holocellulose network. Biological pretreatment methods are used for the delignification of lignocellulosic biomass [42]; however, the longer pretreatment times and loss of a considerable amount of carbohydrates can occur during this pretreatment [43].Each method has its own specificity in terms of mechanistic application on cell wall components with the applied conditions [42]. Some types of pretreatments (like milling, pyrolysis, steam explosion, ammonia fiber explosion, acid or alkaline cooking, organosolv extraction, and so on) are described as follows.


#### 5.1.1. Physical Pretreatments

(1) Milling
Milling is a mechanical pretreatment that breaks down the structure of lignocellulosic materials and decrease the cellulose crystallinity [44]. Ball milling method is most commonly employed, where the contact of the biomass with balls inside a cycle machine reduces the particles size [28]. This method can be considered environment friendly because it does not required chemicals addition [45] and thus inhibitors are not generated [28]. A disadvantage of milling is the high powerrequiredby the machines and consequently high energy costs. For sugarcane bagasse pretreatment is necessary for a lot of cycles and many passes through the miller and the cycles usually have a long time of operation [46].(2) Pyrolysis
The pyrolysis process is carried out at high temperatures (more than 300°C). This process degrades cellulose rapidly into H2, CO, and residual char [43]. After the separation of char, the recovered solution is primarily composed by glucose, which can be eventually fermented for ethanol production [47]. This process starts with the heating of the biomass. Primary pyrolysis reactions initiate at high temperatures to release volatiles, followed by condensation of hot volatiles and proceeded with autocatalytic secondary pyrolysis reactions [48].
The yield and quality of products after pyrolysis will depend on several parameters which can be categorized as process parameters (temperature, heating rate, residence time, reaction time, reactor type, type and amount of catalyst, type of sweeping gas, and flow rate) [48] and feedstock properties (particle size, porosity, cellulose, hemicellulose, and lignin content [49].(3) Microwave
Microwave pretreatment is considered as an alternative process for conventional heating. If compared with conventional heating method that uses superficial transfer of heat, microwave pretreatment uses the direct interaction between a heated object and an applied electromagnetic, generating high heating efficiency and easy operation [50]. The main advantage of this process is the short reactions times and homogeneous heating of the reaction mixture [51]. Microwave assisted pretreatment of SB/SS could be a useful process to save time and energy and minimum generation of inhibitors [52].
It can be considered as one of the most promising pretreatment method to change the native structure of cellulose [53], with the occurrence of the lignin and hemicellulose degradation and thus increasing the enzymatic susceptibility [54]. Microwave can be combined with the chemicals further to improve the sugar yield from the substrate [51].


#### 5.1.2. Physicochemical Pretreatments

(1) Steam Explosion or Hydrothermal
Steam explosion (or hydrothermal) is one of the most common pretreatment methods. This method can be described as a thermochemical process, where lignocellulosic material is exposed with steam [55]. This pretreatment requires minimum, or in some cases, no chemical addition, then it can be seen as a good technology when it is regarding environment concerns [56]. In this process, a mix of biomass and steam is maintained in high temperature in a reactor, promoting the hemicellulose hydrolysis followed by a quickly decompression ending the reaction [57].
Steam explosion treatment yields high solubility of the hemicellulose (producing mainly oligosaccharides) with low lignin solubility [14]. Usually, temperatures between 160 and 240°C and pressure between 0.7 and 4.8 MPa are employed [57]. Steam explosion process followed by enzymatic saccharification is a promising approach to enhance the amount of fermentable sugars.(2) Ammonia Fiber Explosion (AFEX)
AFEX process consists of liquid ammonia and steam explosion. It is a alkaline thermal treatment that exposes the lignocellulosic material to high temperature and pressure followed by fast pressure release. This pretreatment can significantly improve the fermentation rate of various herbaceous crops and grasses [43], and it can be used for the pretreatment of many lignocellulosic materials including alfalfa, wheat straw, wheat chaff, barley straw, corn stover, rice straw, and bagasse [44]. The main advantages of AFEX are the efficient lignin removal and less generation of inhibitors, retaining appreciable amount of carbohydrates in the substrates. Furthermore, it is a simple with short-time process [47]. During the AFEX, structure of the material is changed, resulting in a increase of water holding capacity and of digestibility of substrates (hemicellulose and cellulose) by enzymes, obtaining thus, high sugars recovery [43, 47].
The cost of AFEX process could be minimized if the ammonia is recovered from the lignin-rich solution [43, 47]. The parameters that influence the AFEX process are ammonia loading, temperature, high pressure, moisture content of biomass, and residence time [47, 58].(3) CO2 Explosion
The CO2 explosion occurs similarly to ammonia explosion and is based on the hypothesis that CO2 would form carbonic acid, increasing the hydrolysis rate of the pretreated material [44]. Carbon dioxide molecules are comparable in size to water, penetrating into the bagasse or straw surfaces, improving the hydrolysis of hemicellulose and cellulose fractions. The increase of the pressure during the explosion helps the penetration of CO2 molecules into the crystalline structure of lignocellulosics [43].
CO2 explosion presents conversion yields higher than the steam explosion method, more cost effective than ammonia explosion and does not cause the formation of inhibitors because mild temperature is used during the process, preventing any appreciable decomposition of monosaccharides [43, 59]. This method is nontoxic, noninflammable, and environmental friendly. However, it is a method with hard operation and process complexities [60].(4) Hot Water
This method employs hot water under high pressure in the biomass hydrating the cellulose and removes a considerable part of hemicellulose fraction. One of the main advantage of this process is the no use of chemicals and consequently not necessary to use corrosion-resistant materials in the hydrolysis reactor. In addition, reduction the size of the raw material is also not required [61].
Usually, in this process the hot water is maintained in contact of the biomass for about 15 minutes at a temperature of 200–230°C. During this process, about 40–60% of the total biomass is dissolved, and all hemicellulose is removed. This process is generally used for pretreatment of corn fibers and herbaceous crops [62].


#### 5.1.3. Chemical Pretreatments

(1) Acid Pretreatment
Among all types of chemical pretreatments of biomass, dilute acid hydrolysis is reported as one of the most used and oldest method. The solubilization of hemicellulose occurs at high temperatures, or at high concentrated acid, releasing pentose sugars [14, 63] and facilitating the enzymatic hydrolysis of remaining substrate (cellulignin) [61]. The most commonly used acid is H2SO4, where its contact with biomass promotes hemicellulose breakdown in xylose and other sugars [14]. However, other acids such as HCl [64], phosphoric acid [65], nitric acid [66], and oxalic acid [67] have also shown promising results. The conditions of the process usually can be performed at temperatures among 120–180°C and residence times ranging 15–60 min [63].
One advantage of the acid pretreatment process is the operation at low and medium temperatures and consequently decreasing of energy costs [68]. However, in high concentration of acid problems can occur with equipment corrosion and expensive costs of maintenance [63], and also after this pretreatment, it is necessary to neutralize the hydrolysate before fermentations [14]. Another disadvantage of this process is the possibility of formation of other by-products that are considered inhibitory to microbial fermentation, like furans, furfural, carboxylic acids, formic levulinic and acetic acids, and phenolic compounds. Therefore, a detoxification step is required to remove these inhibitory compounds to increase the fermentability of hydrolysates [69].
To calculate the efficiency of acid hydrolysis, factors like temperature, reaction time and acid concentration must be considered [70]. Overend and Chornet [71] developed an equation that involves the temperature and reaction time, indicating the severity of the pretreatment by combined severity factor (CSF):
(1)CSF=texp[(T-Tref)14.75],
where t is the residence time (min); T is the temperature (°C), and Tref is the reference temperature, usually set to 100°C.(2) Alkaline Pretreatment
Alkaline pretreatment is a delignification process, in which a significant amount of hemicellulose is also solubilized. It employs various bases, including sodium hydroxide, calcium hydroxide (lime), potassium hydroxide, ammonia hydroxide, and sodium hydroxide in combination with hydrogen peroxide or others [72]. The action mechanism of alkaline hydrolysis is believed to be saponification of intermolecular ester bonds crosslinking xylan hemicelluloses and other components [44]. This process utilizes lower temperatures and pressures than other pretreatment technologies; however, pretreatment times are on the order of hours or days [72]. Compared with acid-based pretreatment processes, alkaline processes causes less sugar degradation, and many of the caustic salts can be recovered and/or regenerated [43].
Alkaline pretreatment of lignocellulosic materials causes swelling, leading to an increase in internal surface area, decrease in the degree of polymerization and crystallinity, separation of structural linkages between lignin and carbohydrates, and disruption of the lignin structure [72] making cellulose and hemicellulose available for the enzymatic degradation [47].
The effectiveness of alkaline pretreatment varies, depending on the substrate and treatment conditions. In general, alkaline pretreatment is more effective on hardwood, herbaceous crops, and agricultural residues with low lignin content than softwood with high lignin content [72]. The end residue (mainly cellulose) can be used to produce either paper or cellulose derivatives [47] or the sugars generation upon enzymatic hydrolysis which can eventually be used for ethanol production. Reactor costs are lower than those for acid technologies. However, the use of these more expensive salts in high concentrations is a significant disadvantage that raises environmental concerns and may lead to prohibitive recycling, wastewater treatment, and residual handling costs [59, 72].(3) Oxidative Delignification
Oxidative delignification process causes the delignification and the chemical swelling of the cellulose improving enzymatic saccharification significantly [73]. In this process, the lignin degradation is catalyzed by the peroxidase enzyme with the presence of H2O2 [44]. The oxidative delignification has been successfully operated in a continuous flow operation at high biomass loading (approximately 40% solids) and low H2O2 loading; though it is still a relatively less explored method compared to other thermochemical pretreatments [74]. This pretreatment method has been applied to a large variety of biomass such as corn stover, barley straw, wheat straw, bamboo, rice straw, and sugarcane bagasse [74].(4) Ozonolysis
Ozone can be used to degrade the lignin and hemicellulose fractions from lignocellulosic materials such as wheat straw, bagasse, peanut, pine, cotton straw, and poplar sawdust [43]. Ozone is a powerful oxidant, soluble in water and is readily available. It is highly reactive towards the compounds incorporating conjugated double bonds and functional groups with high electron densities. Therefore, the most likely biomass constituent to be oxidized is lignin due to its high content of C=C bounds [75].
Ozonolysis pretreatment has the advantages of effectively removal of lignin, it does not produce toxic residues for the downstream processes, and the reactions are carried out at ambient temperature and pressure [76]. However, a large amount of ozone is required, making the process expensive [44]. Furthermore, the fact that ozone can be easily decomposed by using a catalytic bed or increasing the temperature means that processes can be designed to minimize environmental pollution [43].(5) Organosolv
Organosolv process seems one of the most promising methods for the pretreatment of lignocellulosics materials [77]. During this process, strong inorganic acid acts as a catalyst, promoting the breakdown of lignin-lignin and carbohydrates-lignin bonds from the biomass [38]. When the lignin is removed, the superficial area and volume of the material are also increased considerably, facilitating the enzyme accessibility and consequently improving the efficiency of the process to achieve fermentable sugars [78].
The organosolv process uses fewer amounts of chemicals to neutralize the hydrolyzate and generates few amounts of wastes compared with other similar process [61]. Chemicals such as NaOH or Na2SO3 could be used as catalyst [79]. High efficiency for lignin removal coupled with the high pressure of carbon dioxide has been observed using this process [80].(6) Wet Oxidation
The wet oxidation process occurs in the presence of oxygen or catalyzed air, where the most used catalyst is the sodium carbonate [81]. Wet oxidation allows obtaining high yields of biomass conversion into monosaccharides with low formation of furan and phenolic aldehydes. In the wet oxidation process, the delignification is reported with the increasing of aliphatic acids. This pretreatment is considered expensive [81]. The major advantage of this pretreatment is the combination with alkalis where it is possible to achieve released sugars without generation of furfural and 5-hydroxymethylfurfural, undesirable compounds for fermentations [82].


#### 5.1.4. Biological Pretreatment

Biological pretreatment is the alternative to chemical pretreatment to alter the structure of lignocellulosic materials. Generally, wood degrading microorganisms like bacteria and brown rot, white rot, and soft rot fungi are employed in the biological pretreatment [72]. This method provides degradation of lignin and hemicellulose making the biomass more amenable to enzyme digestion [47].The most effective microorganism for biological pretreatment of lignocellulosic materials is white rot fungi [47]. These microorganisms degrade lignin through the action of lignin-degrading enzymes such as peroxidases and laccase [43]. Brown rot fungi mainly attack cellulose, while white and soft rot fungi attack both cellulose and lignin [44].This pretreatment is environmental friendly because of its low energy use and mild environmental conditions [59]. The main disadvantages, that is, low efficiency, considerable loss of carbohydrates, long residence time, requirement of careful control of growth conditions, and space restrain its applications. In addition, most ligninolytic microorganisms solubilize/consume not only lignin but also a considerable fraction of hemicellulose and cellulose [72]. To overcome these limitations, biological treatments can be used in combination with other treatments [59]. Wang et al. [83] combined fungal treatment with liquid hot water (LHW) to enhance the enzymatic hydrolysis of Populus tomentosa.


### 5.2. Enzymatic Hydrolysis of Cellulosic Fraction

The general concept of conversion of cellulosic fraction into fermentable sugars involves the pretreatment of the raw material followed by its enzymatic hydrolysis. Enzymatic hydrolysis is an ideal approach for degrading cellulose into reducing sugars because mild reaction conditions (pH between 4.8–5.0 and temperature between 45–50°C) can be used; it does not present corrosion problems in the reactors and result in negligible by-products formation with high sugar yields. However, enzymatic hydrolysis depends on optimized conditions for maximal efficiency (hydrolysis temperature, time, pH, enzyme loading, and substrate concentration) and suffers from end-product inhibition and biomass structural restraints [84, 85]. To overcome the end-product inhibition and reducing the time, hydrolysis and fermentation can be combined, so-called simultaneous saccharification and fermentation (SSF) or simultaneous saccharification and cofermentation (SSCF).The enzymatic hydrolysis of cellulosic fraction requires three classes of cellulolytic enzymes (cellulases): (1) endo-β-1,4-glucanases (EG, E.C. 3.2.1.4) which attacks regions of low crystallinity in the cellulose fiber, creating free chain ends; (2) cellobiohydrolases or exoglucanase (CBH, E.C. 3.2.1.91) which degrades the molecule further by removing cellobiose units from the free chain-ends; (3) β-glucosidases (E.C. 3.2.1.21) which hydrolyses cellobiose to produce glucose [44]. To breakdown the hemicellulose, several enzymes such as xylanase, b-xylosidase, glucuronidase, acetylesterase, galactomannanase, and glucomannanase are required [84]. Cellulase enzymes when acting together with xylanases on delignified SB/SS exhibit a better yield due to the synergistic action of enzymes [86].Both bacteria (Clostridium, Cellulomonas, Bacillus, Thermomonospora, Ruminococcus, Bacteriodes, Erwinia, Acetovibrio, Microbispora, and Streptomyces) and fungi (Sclerotium rolfsii, Phanenerochate chrysosporium and species of Trichoderma, Aspergillus, Schizophyllum and Penicillium) can produce cellulases for the hydrolysis of cellulosic materials [44, 84]. Amongst the cellulase producing microorganisms, Aspergillus and Trichoderma genera are the widely studied.For ethanol production from cellulosic materials, removal of lignin improved significantly the substrate digestibility. Furthermore, due to the lower lignin content, enzyme loadings can be considerably reduced. However, extensive lignin removals by pretreatments add cost to the processes [85].Although enzyme price has decreased due to intensive research to improve their production, enzymes loading during cellulose hydrolysis should be minimized because it also increases the cellulosic ethanol production costs. Thus, finding paths to reduce cellulase loadings would be particularly effective in lowering the process costs. The enzyme source has also a major effect on the hydrolysis efficiency. Therefore, understanding the interaction between cellulases and pretreated biomass is vital to effectively develop low-cost pretreatment and enzyme properties that can lead to competitive ethanol costs [85].


### 5.3. Detoxification (Treatment) of Hemicellulosic Hydrolysates

The main preoccupations in the pretreatment of lignocellulosic materials are to minimize the sugars degradation and subsequently minimize the formation of inhibitory compounds for microbial metabolism, limit the consumption of chemicals, energy and water, and the production of wastes [39]. The inhibitory compounds could be divided into four groups: (1) substances that are released by the hemicellulosic structure, such as acetic acid, which originates in the deacetylation of xylan; (2) phenolic compounds and other aromatic compounds derived from the partial degradation of lignin; (3) the furan derivatives, furfural and 5-hydroxymethylfurfural, resulting from the degradation of pentoses and hexoses, respectively; (4) metals like chromium, copper, iron, and nickel leached from the equipment [69]. These compounds individually as well as synergistically affect the physiology of fermenting microorganisms, therefore, it is essential to eliminate these inhibitory compounds or reduce their concentration to obtain the satisfactory product yields during microbial fermentation of lignocellulose hydrolysates [67].A number of methods like evaporation; neutralization; use of membranes, ion exchange resins, and activated charcoal; enzymatic detoxification using laccases and peroxidases have been attempted to detoxify the hydrolysates aiming ethanol production. Considering that different lignocellulosic hydrolysates have different degrees of inhibition and that microorganisms have different inhibitor tolerances, the methods of detoxification change will depend on the source of the lignocellulosic hydrolysate and the microorganism being used [87]. Several detoxification methods are described in this overview and can be divided into physical, physicochemical, chemical, and biological treatments.


#### 5.3.1. Physical Treatments

(1) Evaporation (Concentration)
The concentration of hydrolysates by vacuum evaporation process is a physical detoxification method which reduces the volatile compounds concentration, including acetic acid, furfural and vanillin [87]. However, this treatment has the disadvantage of increasing the nonvolatile toxic compounds, as extractives [88].(2) Use of Membranes
The use of membranes has several advantages over conventional extraction. Membrane adsorption prevents that the aqueous phase (hydrolysate) is mixed with organic phase (solvent) which is likely to be toxic to microorganisms [89]. The membranes have surface functional groups attached to their internal pores, which may eliminate metabolic inhibitors as acetic acid, 5-hydroxymethylfurfural, furfural, formic, levulinic and sulphuric acid [67].


#### 5.3.2. Physicochemical Treatments

(1) Ion Exchange Resins
The ion exchange resins process has been reported as the most efficient detoxification method. It is known that this process remove lignin-derived inhibitors, acetic acid, and furfurals of hydrolysate, improving significantly the yield fermentation [67]. The main advantage of the use of ion exchange resins is that they can be regenerated and reused without affecting the efficiency of the treatment [65, 90]. However, this method presents some disadvantages: the high pressure drop across the bed that tends to increase during operation due to media deformation; long processing time because of the slow pore diffusion; possible degradation of fragile biological product molecules, and it is difficult to scale-up [91]. The ion exchange resins process also leads to a significant loss of fermentable sugars after the process [67].(2) Neutralization
Considering the low pH of the hydrolysates provided by acid hydrolysis, it is needed that the neutralization of the pH to be close to the fermentations conditions. In this step, phenolics and furfurals are removed due to precipitations [67]. The chemicals employed in the neutralization of hydrolysates are calcium hydroxide and sodium hydroxide. The addition of Ca(OH)2 generates precipitate of CaSO4; therefore, it is desirable to be removed by centrifugation, adding one more stage in the process. During the generation of precipitates it can offer problems in fermentation [92].(3) Overliming
Among different types of detoxification, overliming is reported as the most used method [93]. This process consists in an increase of the pH of acid hydrolysate followed by reduction until a pH desirable to fermentations. The principle of this process is the precipitation of toxic components and the instability of some inhibitors at high pH [69]. This method showed high efficiency towards the removal of inhibitors and is being widely used [90, 94, 95]. This method has been considered promising and economic, revealing good efficiency for the removal of the furans compounds [67].(4) Activated Charcoal
Activated charcoal adsorption is a widely used detoxification method which is considered a low cost and efficient for inhibitors compounds removal. This method removes mainly phenolics compounds and does not provide large changes in the fermentable sugars levels [90]. The ratio of charcoal and hydrolysates, pH, time of contact, and temperature are the important factors for the improvement of this method [88, 96].(5) Extraction with Organic Solvents
Due to large availability of inhibitors such as acetic acid, furfural, vanillin, 4-hydroxybenzoic acid, and low molecular weight phenolics, the solvent extraction has been considered an efficient method of detoxification. The most common solvents used in this process are ethyl acetate, chloroform, and trichloroethylene [97].


#### 5.3.3. Biological Treatment

Biological method uses specific enzymes or microorganisms that act on the inhibitors compounds present in the hydrolysate and change them [87]. In contrast with physical and chemical detoxifications, biological detoxification represents an improvement because little waste is generated and could be performed directly in the fermentation vessel before fermentation [98]. This method is still more feasible, environmental friendly, with fewer side reactions and less energy requirements [99]; however, it presents a long process time [100].The use of enzymes is a very studied and promising method. Laccase and peroxidase enzymes derived from white rot fungi have been found effective for the removal of phenolics compounds from lignocellulosic hydrolysates [99]. The detoxification mechanism of these enzymes probably involves oxidative polymerization of phenolic compounds of low molecular weight [88]; they catalyze the oxidation of substituted phenols, anilines, and aromatic thiols, at the expense of molecular oxygen [101]. The disadvantages of enzyme detoxification are long incubation time and high costs of enzymes; however it has the advantage that is usually conducted at mild conditions (pH 5.0, mesophilic temperature) [102].The use of microorganism has also been applied to remove inhibitors compounds from lignocellulosic hydrolysates [87]. There are several microorganisms which can naturally assimilate inhibitory compounds, including yeasts, fungi, and bacteria [102]. Some microorganisms during incubation are able to release cellulase and hemicellulase and degrade only lignin, resulting in a lignocellulosic substrate which can be easily hydrolyzed into fermentable sugars with mild conditions and short time [99].These microorganisms can effectively degrade lignin while retaining cellulose and hemicellulose in the substrate. This method can also be referredas in situ microbial delignification (ISMD). Recently, several microorganisms have shown their preference towards inhibitors by transforming their chemical nature and can be employed for detoxification of lignocellulose hydrolysates [99, 103]. The adaptation of a microorganism to a nondetoxified hydrolysate is another interesting alternative to replace the detoxification step. This method is based on successive fermentations using the microorganism of each experiment as the inoculum of the next one [88]. The use of adapted microorganisms not only reduces the detoxification cost but also avoids loss of fermentable sugars [102].


### 5.4. Fermentation of Sugars from Sugarcane Biomass into Ethanol

#### 5.4.1.  Bioconversion of Hexose Sugars into Ethanol

Ethanol fermentation is a biological process in which sugars are converted by microorganisms to produce ethanol and CO2. Even though there are the existence of many methods and process to use lignocellulosic materials for ethanol production, however, it is still difficult to obtain economic ethanol from lignocellulosics [86].The microorganism most commonly used in fermentation process is the yeasts and, among the yeasts,Saccharomyces cerevisiae is the preferred choice for ethanol fermentation [104]. This yeast can grow both on simple sugars, such as glucose, and on the disaccharide sucrose. Furthermore, the availability of a robust genetic transformation system of S. cerevisiae along with a long history of this microorganism in industrial fermentation processes makes it most desired microorganisms for ethanol production. S. cerevisiae has high resistance to ethanol, consumes significant amounts of substrate in adverse conditions, and shows high resistance to inhibitors present in the medium [105]. Unfortunately, xylose metabolism presents a unique challenge for S. cerevisiae to assimilate pentose sugars due to the absence of genes required for assimilation of these molecules [105].There are three kinds of processes to produce ethanol from sugarcane bagasse (SB) and sugarcane straw (SS). The first process is called separate (or sequential) hydrolysis and fermentation (SHF) where hydrolysis of lignocellulosic material and ethanol fermentation is done separately. SB/SS is pretreated, and the pretreated material is enzymatically hydrolyzed separately to recover the sugars. The recovered sugar solution (hexose sugars) is then fermented with appropriate microorganism into ethanol. SHF is a little staggered process [21]. The other two kinds of processes are called simultaneous saccharification and fermentation (SSF) and simultaneous saccharification and cofermentation (SSCF), where both enzymatic hydrolysis and fermentation of released sugars into ethanol occur simultaneously making the overall process short [21, 86]. In the SSF process, the glucose (from cellulose hydrolyzed) is fermented separately of pentoses (from hydrolysate) in a separate reactor, while that in the SSCF process, the fermentation of xylose and glucose occurs together in the same reactor [21, 86].


#### 5.4.2. Bioconversion of Pentose Sugars into Ethanol

The maximum utilization of all sugar fractions is essential to obtain an economic and viable conversion technology for bioethanol production from sugarcane bagasse (SB) and sugarcane straw (SS). To obtain the desired ethanol yields from SB/SS hydrolysates, it is essential that the hemicellulose fraction should be fermented with same conversion rates as the cellulose fraction [104].Hemicellulose hydrolysate typically contains primarily pentose sugars (xylose and arabinose) and some amounts of hexose sugars (mannose, glucose and galactose) [106, 107]. A variety of yeast, fungi, and bacteria are capable of assimilating pentose, but only a few are promising candidates for the efficient xylose fermentation into ethanol [106]. In yeasts, the assimilation of D-xylose follows the pathway where the sugar passes through a pool enzymatic to enter in the phosphopentose pathway [108]. There are several microorganisms capable of assimilating pentose sugars, but only few species are capable of assimilating sugars to produce ethanol at industrial scale. Microorganisms, such as Scheffersomyces stipitis (Pichia stipitis) [109], Candida guilliermondii, Candida shehatae, and Pachysolen tannophilus are able to assimilate pentose sugars by a reduction/oxidation pathway bioconversion of sugarcane bagasse/sugarcane straw hydrolysates under different cultivation conditions [110].The process to assimilate pentose sugars consists in the xylose being converted to xylitol, by the action of D-xylose reductase (E.C. 1.1.1.21) and immediately oxidized by the action of xylitol dehydrogenase (E.C. 1.1.1.9), producing D-xylose-5-phosphate. Ribolosephosphate-3-epimerase (5.1.3.1), transaldolase (E.C. 2.2.1.2), and transketolase (E.C. 2.2.1.1) sequentially convert D-xylose-5-phosphate into glyceraldehyde-3-phosphate and fructose-6-phoshate by non-oxidative rearrangement resulting into the formation of ethanol by the Emden-Meyorhoff Pathway. NADPH must be regenerated through metabolic routes. The metabolic pathway of the arabinose is similar to the route shown by xylose, where aldose reductase mechanistically converts arabinose into L-arabitol. Through the action of L-arabitol dehydrogenase, L-arabitol is reduced to ethanol [99, 105]. Recently published reviews have competently presented the important progress made for pentose sugars fermentation into ethanol [99, 110].


### 5.5. Distillation of Ethanol

Despite the downstream process being the highest energy consuming process during the ethanol production [111], the ethanol recovery from the fermented broth is necessary. The final medium is composed by water and ethanol (5–12 wt%) [112]. The ethanol-water cannot be separated by conventional distillation processes because they form a nonideal mixture system [113]. The dehydration is sophisticated method because they form an azeotropic mixture with water (at 95.6 wt% at a temperature of 78.15°C), which makes it impossible to separate in a single distillation column [112]. This way, the ethanol purification occurs in three steps: distillation, rectification, and dehydration. A high concentrated ethanol solution is obtained in the two first steps (about 92.4 wt%), then the mixture is dehydrated in order to obtain ethanol anhydrous by a dehydration method. The dehydration can be realized by azeotropic distillation, extractive distillation, liquid-liquid extraction, adsorption, or some complex hybrid separation methods [112].


## 5.1. Pretreatment of Sugarcane Biomass

Ideally, the pretreatment of lignocellulosic biomass should (1) increase the accessible surface area and decrystallize cellulose, (2) depolymerize partially cellulose, (3) solubilize hemicellulose and/or lignin, (4) modify the lignin structure, (5) maximize the enzymatic digestibility of the pretreated material, (6) minimize the loss of sugars, and (7) minimize capital and operating costs [38, 39].Figure4 presents scanning electronic microscopy (SEM) of SB before diluted sulfuric acid pretreatment and of cellulignin obtained after pretreatment. A rupture of cellulose-hemicellulose-lignin strong matrix occurred after the pretreatment. In the Figure 4(a), an ordered structure of matrix can be seen, while Figure 4(b) presents a disordered structure of cellulose-lignin complex. It is also possible to find empty spaces between the fibers, as consequence of removal of hemicelluloses and low-crystallinity cellulose flocks [15]. In general, hydrolysate originated after diluted acid pretreatment is rich in the hemicellulose fraction.SEM of natural SB (a) and cellulignin obtained after dilute sulfuric acid pretreatment (b) [40].
(a)
(b)Various pretreatment technologies (alone or in combination) have been proposed in the literature. Broadly, pretreatment technologies can be categorized into 4 types: physical (mechanical); physicochemical; chemical; biological pretreatments. Mechanical pretreatment increases the surface area by reducing the size the SB or SS [41]. A high control of operation conditions is required in the physicochemical methods because these reactions occur at high temperature and pressure [21]. Chemical methods degrade hemicellulose or remove lignin and thus, loosening the structural of lignin-holocellulose network. Biological pretreatment methods are used for the delignification of lignocellulosic biomass [42]; however, the longer pretreatment times and loss of a considerable amount of carbohydrates can occur during this pretreatment [43].Each method has its own specificity in terms of mechanistic application on cell wall components with the applied conditions [42]. Some types of pretreatments (like milling, pyrolysis, steam explosion, ammonia fiber explosion, acid or alkaline cooking, organosolv extraction, and so on) are described as follows.


### 5.1.1. Physical Pretreatments

(1) Milling
Milling is a mechanical pretreatment that breaks down the structure of lignocellulosic materials and decrease the cellulose crystallinity [44]. Ball milling method is most commonly employed, where the contact of the biomass with balls inside a cycle machine reduces the particles size [28]. This method can be considered environment friendly because it does not required chemicals addition [45] and thus inhibitors are not generated [28]. A disadvantage of milling is the high powerrequiredby the machines and consequently high energy costs. For sugarcane bagasse pretreatment is necessary for a lot of cycles and many passes through the miller and the cycles usually have a long time of operation [46].(2) Pyrolysis
The pyrolysis process is carried out at high temperatures (more than 300°C). This process degrades cellulose rapidly into H2, CO, and residual char [43]. After the separation of char, the recovered solution is primarily composed by glucose, which can be eventually fermented for ethanol production [47]. This process starts with the heating of the biomass. Primary pyrolysis reactions initiate at high temperatures to release volatiles, followed by condensation of hot volatiles and proceeded with autocatalytic secondary pyrolysis reactions [48].
The yield and quality of products after pyrolysis will depend on several parameters which can be categorized as process parameters (temperature, heating rate, residence time, reaction time, reactor type, type and amount of catalyst, type of sweeping gas, and flow rate) [48] and feedstock properties (particle size, porosity, cellulose, hemicellulose, and lignin content [49].(3) Microwave
Microwave pretreatment is considered as an alternative process for conventional heating. If compared with conventional heating method that uses superficial transfer of heat, microwave pretreatment uses the direct interaction between a heated object and an applied electromagnetic, generating high heating efficiency and easy operation [50]. The main advantage of this process is the short reactions times and homogeneous heating of the reaction mixture [51]. Microwave assisted pretreatment of SB/SS could be a useful process to save time and energy and minimum generation of inhibitors [52].
It can be considered as one of the most promising pretreatment method to change the native structure of cellulose [53], with the occurrence of the lignin and hemicellulose degradation and thus increasing the enzymatic susceptibility [54]. Microwave can be combined with the chemicals further to improve the sugar yield from the substrate [51].


### 5.1.2. Physicochemical Pretreatments

(1) Steam Explosion or Hydrothermal
Steam explosion (or hydrothermal) is one of the most common pretreatment methods. This method can be described as a thermochemical process, where lignocellulosic material is exposed with steam [55]. This pretreatment requires minimum, or in some cases, no chemical addition, then it can be seen as a good technology when it is regarding environment concerns [56]. In this process, a mix of biomass and steam is maintained in high temperature in a reactor, promoting the hemicellulose hydrolysis followed by a quickly decompression ending the reaction [57].
Steam explosion treatment yields high solubility of the hemicellulose (producing mainly oligosaccharides) with low lignin solubility [14]. Usually, temperatures between 160 and 240°C and pressure between 0.7 and 4.8 MPa are employed [57]. Steam explosion process followed by enzymatic saccharification is a promising approach to enhance the amount of fermentable sugars.(2) Ammonia Fiber Explosion (AFEX)
AFEX process consists of liquid ammonia and steam explosion. It is a alkaline thermal treatment that exposes the lignocellulosic material to high temperature and pressure followed by fast pressure release. This pretreatment can significantly improve the fermentation rate of various herbaceous crops and grasses [43], and it can be used for the pretreatment of many lignocellulosic materials including alfalfa, wheat straw, wheat chaff, barley straw, corn stover, rice straw, and bagasse [44]. The main advantages of AFEX are the efficient lignin removal and less generation of inhibitors, retaining appreciable amount of carbohydrates in the substrates. Furthermore, it is a simple with short-time process [47]. During the AFEX, structure of the material is changed, resulting in a increase of water holding capacity and of digestibility of substrates (hemicellulose and cellulose) by enzymes, obtaining thus, high sugars recovery [43, 47].
The cost of AFEX process could be minimized if the ammonia is recovered from the lignin-rich solution [43, 47]. The parameters that influence the AFEX process are ammonia loading, temperature, high pressure, moisture content of biomass, and residence time [47, 58].(3) CO2 Explosion
The CO2 explosion occurs similarly to ammonia explosion and is based on the hypothesis that CO2 would form carbonic acid, increasing the hydrolysis rate of the pretreated material [44]. Carbon dioxide molecules are comparable in size to water, penetrating into the bagasse or straw surfaces, improving the hydrolysis of hemicellulose and cellulose fractions. The increase of the pressure during the explosion helps the penetration of CO2 molecules into the crystalline structure of lignocellulosics [43].
CO2 explosion presents conversion yields higher than the steam explosion method, more cost effective than ammonia explosion and does not cause the formation of inhibitors because mild temperature is used during the process, preventing any appreciable decomposition of monosaccharides [43, 59]. This method is nontoxic, noninflammable, and environmental friendly. However, it is a method with hard operation and process complexities [60].(4) Hot Water
This method employs hot water under high pressure in the biomass hydrating the cellulose and removes a considerable part of hemicellulose fraction. One of the main advantage of this process is the no use of chemicals and consequently not necessary to use corrosion-resistant materials in the hydrolysis reactor. In addition, reduction the size of the raw material is also not required [61].
Usually, in this process the hot water is maintained in contact of the biomass for about 15 minutes at a temperature of 200–230°C. During this process, about 40–60% of the total biomass is dissolved, and all hemicellulose is removed. This process is generally used for pretreatment of corn fibers and herbaceous crops [62].


### 5.1.3. Chemical Pretreatments

(1) Acid Pretreatment
Among all types of chemical pretreatments of biomass, dilute acid hydrolysis is reported as one of the most used and oldest method. The solubilization of hemicellulose occurs at high temperatures, or at high concentrated acid, releasing pentose sugars [14, 63] and facilitating the enzymatic hydrolysis of remaining substrate (cellulignin) [61]. The most commonly used acid is H2SO4, where its contact with biomass promotes hemicellulose breakdown in xylose and other sugars [14]. However, other acids such as HCl [64], phosphoric acid [65], nitric acid [66], and oxalic acid [67] have also shown promising results. The conditions of the process usually can be performed at temperatures among 120–180°C and residence times ranging 15–60 min [63].
One advantage of the acid pretreatment process is the operation at low and medium temperatures and consequently decreasing of energy costs [68]. However, in high concentration of acid problems can occur with equipment corrosion and expensive costs of maintenance [63], and also after this pretreatment, it is necessary to neutralize the hydrolysate before fermentations [14]. Another disadvantage of this process is the possibility of formation of other by-products that are considered inhibitory to microbial fermentation, like furans, furfural, carboxylic acids, formic levulinic and acetic acids, and phenolic compounds. Therefore, a detoxification step is required to remove these inhibitory compounds to increase the fermentability of hydrolysates [69].
To calculate the efficiency of acid hydrolysis, factors like temperature, reaction time and acid concentration must be considered [70]. Overend and Chornet [71] developed an equation that involves the temperature and reaction time, indicating the severity of the pretreatment by combined severity factor (CSF):
(1)CSF=texp[(T-Tref)14.75],
where t is the residence time (min); T is the temperature (°C), and Tref is the reference temperature, usually set to 100°C.(2) Alkaline Pretreatment
Alkaline pretreatment is a delignification process, in which a significant amount of hemicellulose is also solubilized. It employs various bases, including sodium hydroxide, calcium hydroxide (lime), potassium hydroxide, ammonia hydroxide, and sodium hydroxide in combination with hydrogen peroxide or others [72]. The action mechanism of alkaline hydrolysis is believed to be saponification of intermolecular ester bonds crosslinking xylan hemicelluloses and other components [44]. This process utilizes lower temperatures and pressures than other pretreatment technologies; however, pretreatment times are on the order of hours or days [72]. Compared with acid-based pretreatment processes, alkaline processes causes less sugar degradation, and many of the caustic salts can be recovered and/or regenerated [43].
Alkaline pretreatment of lignocellulosic materials causes swelling, leading to an increase in internal surface area, decrease in the degree of polymerization and crystallinity, separation of structural linkages between lignin and carbohydrates, and disruption of the lignin structure [72] making cellulose and hemicellulose available for the enzymatic degradation [47].
The effectiveness of alkaline pretreatment varies, depending on the substrate and treatment conditions. In general, alkaline pretreatment is more effective on hardwood, herbaceous crops, and agricultural residues with low lignin content than softwood with high lignin content [72]. The end residue (mainly cellulose) can be used to produce either paper or cellulose derivatives [47] or the sugars generation upon enzymatic hydrolysis which can eventually be used for ethanol production. Reactor costs are lower than those for acid technologies. However, the use of these more expensive salts in high concentrations is a significant disadvantage that raises environmental concerns and may lead to prohibitive recycling, wastewater treatment, and residual handling costs [59, 72].(3) Oxidative Delignification
Oxidative delignification process causes the delignification and the chemical swelling of the cellulose improving enzymatic saccharification significantly [73]. In this process, the lignin degradation is catalyzed by the peroxidase enzyme with the presence of H2O2 [44]. The oxidative delignification has been successfully operated in a continuous flow operation at high biomass loading (approximately 40% solids) and low H2O2 loading; though it is still a relatively less explored method compared to other thermochemical pretreatments [74]. This pretreatment method has been applied to a large variety of biomass such as corn stover, barley straw, wheat straw, bamboo, rice straw, and sugarcane bagasse [74].(4) Ozonolysis
Ozone can be used to degrade the lignin and hemicellulose fractions from lignocellulosic materials such as wheat straw, bagasse, peanut, pine, cotton straw, and poplar sawdust [43]. Ozone is a powerful oxidant, soluble in water and is readily available. It is highly reactive towards the compounds incorporating conjugated double bonds and functional groups with high electron densities. Therefore, the most likely biomass constituent to be oxidized is lignin due to its high content of C=C bounds [75].
Ozonolysis pretreatment has the advantages of effectively removal of lignin, it does not produce toxic residues for the downstream processes, and the reactions are carried out at ambient temperature and pressure [76]. However, a large amount of ozone is required, making the process expensive [44]. Furthermore, the fact that ozone can be easily decomposed by using a catalytic bed or increasing the temperature means that processes can be designed to minimize environmental pollution [43].(5) Organosolv
Organosolv process seems one of the most promising methods for the pretreatment of lignocellulosics materials [77]. During this process, strong inorganic acid acts as a catalyst, promoting the breakdown of lignin-lignin and carbohydrates-lignin bonds from the biomass [38]. When the lignin is removed, the superficial area and volume of the material are also increased considerably, facilitating the enzyme accessibility and consequently improving the efficiency of the process to achieve fermentable sugars [78].
The organosolv process uses fewer amounts of chemicals to neutralize the hydrolyzate and generates few amounts of wastes compared with other similar process [61]. Chemicals such as NaOH or Na2SO3 could be used as catalyst [79]. High efficiency for lignin removal coupled with the high pressure of carbon dioxide has been observed using this process [80].(6) Wet Oxidation
The wet oxidation process occurs in the presence of oxygen or catalyzed air, where the most used catalyst is the sodium carbonate [81]. Wet oxidation allows obtaining high yields of biomass conversion into monosaccharides with low formation of furan and phenolic aldehydes. In the wet oxidation process, the delignification is reported with the increasing of aliphatic acids. This pretreatment is considered expensive [81]. The major advantage of this pretreatment is the combination with alkalis where it is possible to achieve released sugars without generation of furfural and 5-hydroxymethylfurfural, undesirable compounds for fermentations [82].


### 5.1.4. Biological Pretreatment

Biological pretreatment is the alternative to chemical pretreatment to alter the structure of lignocellulosic materials. Generally, wood degrading microorganisms like bacteria and brown rot, white rot, and soft rot fungi are employed in the biological pretreatment [72]. This method provides degradation of lignin and hemicellulose making the biomass more amenable to enzyme digestion [47].The most effective microorganism for biological pretreatment of lignocellulosic materials is white rot fungi [47]. These microorganisms degrade lignin through the action of lignin-degrading enzymes such as peroxidases and laccase [43]. Brown rot fungi mainly attack cellulose, while white and soft rot fungi attack both cellulose and lignin [44].This pretreatment is environmental friendly because of its low energy use and mild environmental conditions [59]. The main disadvantages, that is, low efficiency, considerable loss of carbohydrates, long residence time, requirement of careful control of growth conditions, and space restrain its applications. In addition, most ligninolytic microorganisms solubilize/consume not only lignin but also a considerable fraction of hemicellulose and cellulose [72]. To overcome these limitations, biological treatments can be used in combination with other treatments [59]. Wang et al. [83] combined fungal treatment with liquid hot water (LHW) to enhance the enzymatic hydrolysis of Populus tomentosa.


## 5.1.1. Physical Pretreatments

(1) Milling
Milling is a mechanical pretreatment that breaks down the structure of lignocellulosic materials and decrease the cellulose crystallinity [44]. Ball milling method is most commonly employed, where the contact of the biomass with balls inside a cycle machine reduces the particles size [28]. This method can be considered environment friendly because it does not required chemicals addition [45] and thus inhibitors are not generated [28]. A disadvantage of milling is the high powerrequiredby the machines and consequently high energy costs. For sugarcane bagasse pretreatment is necessary for a lot of cycles and many passes through the miller and the cycles usually have a long time of operation [46].(2) Pyrolysis
The pyrolysis process is carried out at high temperatures (more than 300°C). This process degrades cellulose rapidly into H2, CO, and residual char [43]. After the separation of char, the recovered solution is primarily composed by glucose, which can be eventually fermented for ethanol production [47]. This process starts with the heating of the biomass. Primary pyrolysis reactions initiate at high temperatures to release volatiles, followed by condensation of hot volatiles and proceeded with autocatalytic secondary pyrolysis reactions [48].
The yield and quality of products after pyrolysis will depend on several parameters which can be categorized as process parameters (temperature, heating rate, residence time, reaction time, reactor type, type and amount of catalyst, type of sweeping gas, and flow rate) [48] and feedstock properties (particle size, porosity, cellulose, hemicellulose, and lignin content [49].(3) Microwave
Microwave pretreatment is considered as an alternative process for conventional heating. If compared with conventional heating method that uses superficial transfer of heat, microwave pretreatment uses the direct interaction between a heated object and an applied electromagnetic, generating high heating efficiency and easy operation [50]. The main advantage of this process is the short reactions times and homogeneous heating of the reaction mixture [51]. Microwave assisted pretreatment of SB/SS could be a useful process to save time and energy and minimum generation of inhibitors [52].
It can be considered as one of the most promising pretreatment method to change the native structure of cellulose [53], with the occurrence of the lignin and hemicellulose degradation and thus increasing the enzymatic susceptibility [54]. Microwave can be combined with the chemicals further to improve the sugar yield from the substrate [51].


## 5.1.2. Physicochemical Pretreatments

(1) Steam Explosion or Hydrothermal
Steam explosion (or hydrothermal) is one of the most common pretreatment methods. This method can be described as a thermochemical process, where lignocellulosic material is exposed with steam [55]. This pretreatment requires minimum, or in some cases, no chemical addition, then it can be seen as a good technology when it is regarding environment concerns [56]. In this process, a mix of biomass and steam is maintained in high temperature in a reactor, promoting the hemicellulose hydrolysis followed by a quickly decompression ending the reaction [57].
Steam explosion treatment yields high solubility of the hemicellulose (producing mainly oligosaccharides) with low lignin solubility [14]. Usually, temperatures between 160 and 240°C and pressure between 0.7 and 4.8 MPa are employed [57]. Steam explosion process followed by enzymatic saccharification is a promising approach to enhance the amount of fermentable sugars.(2) Ammonia Fiber Explosion (AFEX)
AFEX process consists of liquid ammonia and steam explosion. It is a alkaline thermal treatment that exposes the lignocellulosic material to high temperature and pressure followed by fast pressure release. This pretreatment can significantly improve the fermentation rate of various herbaceous crops and grasses [43], and it can be used for the pretreatment of many lignocellulosic materials including alfalfa, wheat straw, wheat chaff, barley straw, corn stover, rice straw, and bagasse [44]. The main advantages of AFEX are the efficient lignin removal and less generation of inhibitors, retaining appreciable amount of carbohydrates in the substrates. Furthermore, it is a simple with short-time process [47]. During the AFEX, structure of the material is changed, resulting in a increase of water holding capacity and of digestibility of substrates (hemicellulose and cellulose) by enzymes, obtaining thus, high sugars recovery [43, 47].
The cost of AFEX process could be minimized if the ammonia is recovered from the lignin-rich solution [43, 47]. The parameters that influence the AFEX process are ammonia loading, temperature, high pressure, moisture content of biomass, and residence time [47, 58].(3) CO2 Explosion
The CO2 explosion occurs similarly to ammonia explosion and is based on the hypothesis that CO2 would form carbonic acid, increasing the hydrolysis rate of the pretreated material [44]. Carbon dioxide molecules are comparable in size to water, penetrating into the bagasse or straw surfaces, improving the hydrolysis of hemicellulose and cellulose fractions. The increase of the pressure during the explosion helps the penetration of CO2 molecules into the crystalline structure of lignocellulosics [43].
CO2 explosion presents conversion yields higher than the steam explosion method, more cost effective than ammonia explosion and does not cause the formation of inhibitors because mild temperature is used during the process, preventing any appreciable decomposition of monosaccharides [43, 59]. This method is nontoxic, noninflammable, and environmental friendly. However, it is a method with hard operation and process complexities [60].(4) Hot Water
This method employs hot water under high pressure in the biomass hydrating the cellulose and removes a considerable part of hemicellulose fraction. One of the main advantage of this process is the no use of chemicals and consequently not necessary to use corrosion-resistant materials in the hydrolysis reactor. In addition, reduction the size of the raw material is also not required [61].
Usually, in this process the hot water is maintained in contact of the biomass for about 15 minutes at a temperature of 200–230°C. During this process, about 40–60% of the total biomass is dissolved, and all hemicellulose is removed. This process is generally used for pretreatment of corn fibers and herbaceous crops [62].


## 5.1.3. Chemical Pretreatments

(1) Acid Pretreatment
Among all types of chemical pretreatments of biomass, dilute acid hydrolysis is reported as one of the most used and oldest method. The solubilization of hemicellulose occurs at high temperatures, or at high concentrated acid, releasing pentose sugars [14, 63] and facilitating the enzymatic hydrolysis of remaining substrate (cellulignin) [61]. The most commonly used acid is H2SO4, where its contact with biomass promotes hemicellulose breakdown in xylose and other sugars [14]. However, other acids such as HCl [64], phosphoric acid [65], nitric acid [66], and oxalic acid [67] have also shown promising results. The conditions of the process usually can be performed at temperatures among 120–180°C and residence times ranging 15–60 min [63].
One advantage of the acid pretreatment process is the operation at low and medium temperatures and consequently decreasing of energy costs [68]. However, in high concentration of acid problems can occur with equipment corrosion and expensive costs of maintenance [63], and also after this pretreatment, it is necessary to neutralize the hydrolysate before fermentations [14]. Another disadvantage of this process is the possibility of formation of other by-products that are considered inhibitory to microbial fermentation, like furans, furfural, carboxylic acids, formic levulinic and acetic acids, and phenolic compounds. Therefore, a detoxification step is required to remove these inhibitory compounds to increase the fermentability of hydrolysates [69].
To calculate the efficiency of acid hydrolysis, factors like temperature, reaction time and acid concentration must be considered [70]. Overend and Chornet [71] developed an equation that involves the temperature and reaction time, indicating the severity of the pretreatment by combined severity factor (CSF):
(1)CSF=texp[(T-Tref)14.75],
where t is the residence time (min); T is the temperature (°C), and Tref is the reference temperature, usually set to 100°C.(2) Alkaline Pretreatment
Alkaline pretreatment is a delignification process, in which a significant amount of hemicellulose is also solubilized. It employs various bases, including sodium hydroxide, calcium hydroxide (lime), potassium hydroxide, ammonia hydroxide, and sodium hydroxide in combination with hydrogen peroxide or others [72]. The action mechanism of alkaline hydrolysis is believed to be saponification of intermolecular ester bonds crosslinking xylan hemicelluloses and other components [44]. This process utilizes lower temperatures and pressures than other pretreatment technologies; however, pretreatment times are on the order of hours or days [72]. Compared with acid-based pretreatment processes, alkaline processes causes less sugar degradation, and many of the caustic salts can be recovered and/or regenerated [43].
Alkaline pretreatment of lignocellulosic materials causes swelling, leading to an increase in internal surface area, decrease in the degree of polymerization and crystallinity, separation of structural linkages between lignin and carbohydrates, and disruption of the lignin structure [72] making cellulose and hemicellulose available for the enzymatic degradation [47].
The effectiveness of alkaline pretreatment varies, depending on the substrate and treatment conditions. In general, alkaline pretreatment is more effective on hardwood, herbaceous crops, and agricultural residues with low lignin content than softwood with high lignin content [72]. The end residue (mainly cellulose) can be used to produce either paper or cellulose derivatives [47] or the sugars generation upon enzymatic hydrolysis which can eventually be used for ethanol production. Reactor costs are lower than those for acid technologies. However, the use of these more expensive salts in high concentrations is a significant disadvantage that raises environmental concerns and may lead to prohibitive recycling, wastewater treatment, and residual handling costs [59, 72].(3) Oxidative Delignification
Oxidative delignification process causes the delignification and the chemical swelling of the cellulose improving enzymatic saccharification significantly [73]. In this process, the lignin degradation is catalyzed by the peroxidase enzyme with the presence of H2O2 [44]. The oxidative delignification has been successfully operated in a continuous flow operation at high biomass loading (approximately 40% solids) and low H2O2 loading; though it is still a relatively less explored method compared to other thermochemical pretreatments [74]. This pretreatment method has been applied to a large variety of biomass such as corn stover, barley straw, wheat straw, bamboo, rice straw, and sugarcane bagasse [74].(4) Ozonolysis
Ozone can be used to degrade the lignin and hemicellulose fractions from lignocellulosic materials such as wheat straw, bagasse, peanut, pine, cotton straw, and poplar sawdust [43]. Ozone is a powerful oxidant, soluble in water and is readily available. It is highly reactive towards the compounds incorporating conjugated double bonds and functional groups with high electron densities. Therefore, the most likely biomass constituent to be oxidized is lignin due to its high content of C=C bounds [75].
Ozonolysis pretreatment has the advantages of effectively removal of lignin, it does not produce toxic residues for the downstream processes, and the reactions are carried out at ambient temperature and pressure [76]. However, a large amount of ozone is required, making the process expensive [44]. Furthermore, the fact that ozone can be easily decomposed by using a catalytic bed or increasing the temperature means that processes can be designed to minimize environmental pollution [43].(5) Organosolv
Organosolv process seems one of the most promising methods for the pretreatment of lignocellulosics materials [77]. During this process, strong inorganic acid acts as a catalyst, promoting the breakdown of lignin-lignin and carbohydrates-lignin bonds from the biomass [38]. When the lignin is removed, the superficial area and volume of the material are also increased considerably, facilitating the enzyme accessibility and consequently improving the efficiency of the process to achieve fermentable sugars [78].
The organosolv process uses fewer amounts of chemicals to neutralize the hydrolyzate and generates few amounts of wastes compared with other similar process [61]. Chemicals such as NaOH or Na2SO3 could be used as catalyst [79]. High efficiency for lignin removal coupled with the high pressure of carbon dioxide has been observed using this process [80].(6) Wet Oxidation
The wet oxidation process occurs in the presence of oxygen or catalyzed air, where the most used catalyst is the sodium carbonate [81]. Wet oxidation allows obtaining high yields of biomass conversion into monosaccharides with low formation of furan and phenolic aldehydes. In the wet oxidation process, the delignification is reported with the increasing of aliphatic acids. This pretreatment is considered expensive [81]. The major advantage of this pretreatment is the combination with alkalis where it is possible to achieve released sugars without generation of furfural and 5-hydroxymethylfurfural, undesirable compounds for fermentations [82].


## 5.1.4. Biological Pretreatment

Biological pretreatment is the alternative to chemical pretreatment to alter the structure of lignocellulosic materials. Generally, wood degrading microorganisms like bacteria and brown rot, white rot, and soft rot fungi are employed in the biological pretreatment [72]. This method provides degradation of lignin and hemicellulose making the biomass more amenable to enzyme digestion [47].The most effective microorganism for biological pretreatment of lignocellulosic materials is white rot fungi [47]. These microorganisms degrade lignin through the action of lignin-degrading enzymes such as peroxidases and laccase [43]. Brown rot fungi mainly attack cellulose, while white and soft rot fungi attack both cellulose and lignin [44].This pretreatment is environmental friendly because of its low energy use and mild environmental conditions [59]. The main disadvantages, that is, low efficiency, considerable loss of carbohydrates, long residence time, requirement of careful control of growth conditions, and space restrain its applications. In addition, most ligninolytic microorganisms solubilize/consume not only lignin but also a considerable fraction of hemicellulose and cellulose [72]. To overcome these limitations, biological treatments can be used in combination with other treatments [59]. Wang et al. [83] combined fungal treatment with liquid hot water (LHW) to enhance the enzymatic hydrolysis of Populus tomentosa.


## 5.2. Enzymatic Hydrolysis of Cellulosic Fraction

The general concept of conversion of cellulosic fraction into fermentable sugars involves the pretreatment of the raw material followed by its enzymatic hydrolysis. Enzymatic hydrolysis is an ideal approach for degrading cellulose into reducing sugars because mild reaction conditions (pH between 4.8–5.0 and temperature between 45–50°C) can be used; it does not present corrosion problems in the reactors and result in negligible by-products formation with high sugar yields. However, enzymatic hydrolysis depends on optimized conditions for maximal efficiency (hydrolysis temperature, time, pH, enzyme loading, and substrate concentration) and suffers from end-product inhibition and biomass structural restraints [84, 85]. To overcome the end-product inhibition and reducing the time, hydrolysis and fermentation can be combined, so-called simultaneous saccharification and fermentation (SSF) or simultaneous saccharification and cofermentation (SSCF).The enzymatic hydrolysis of cellulosic fraction requires three classes of cellulolytic enzymes (cellulases): (1) endo-β-1,4-glucanases (EG, E.C. 3.2.1.4) which attacks regions of low crystallinity in the cellulose fiber, creating free chain ends; (2) cellobiohydrolases or exoglucanase (CBH, E.C. 3.2.1.91) which degrades the molecule further by removing cellobiose units from the free chain-ends; (3) β-glucosidases (E.C. 3.2.1.21) which hydrolyses cellobiose to produce glucose [44]. To breakdown the hemicellulose, several enzymes such as xylanase, b-xylosidase, glucuronidase, acetylesterase, galactomannanase, and glucomannanase are required [84]. Cellulase enzymes when acting together with xylanases on delignified SB/SS exhibit a better yield due to the synergistic action of enzymes [86].Both bacteria (Clostridium, Cellulomonas, Bacillus, Thermomonospora, Ruminococcus, Bacteriodes, Erwinia, Acetovibrio, Microbispora, and Streptomyces) and fungi (Sclerotium rolfsii, Phanenerochate chrysosporium and species of Trichoderma, Aspergillus, Schizophyllum and Penicillium) can produce cellulases for the hydrolysis of cellulosic materials [44, 84]. Amongst the cellulase producing microorganisms, Aspergillus and Trichoderma genera are the widely studied.For ethanol production from cellulosic materials, removal of lignin improved significantly the substrate digestibility. Furthermore, due to the lower lignin content, enzyme loadings can be considerably reduced. However, extensive lignin removals by pretreatments add cost to the processes [85].Although enzyme price has decreased due to intensive research to improve their production, enzymes loading during cellulose hydrolysis should be minimized because it also increases the cellulosic ethanol production costs. Thus, finding paths to reduce cellulase loadings would be particularly effective in lowering the process costs. The enzyme source has also a major effect on the hydrolysis efficiency. Therefore, understanding the interaction between cellulases and pretreated biomass is vital to effectively develop low-cost pretreatment and enzyme properties that can lead to competitive ethanol costs [85].


## 5.3. Detoxification (Treatment) of Hemicellulosic Hydrolysates

The main preoccupations in the pretreatment of lignocellulosic materials are to minimize the sugars degradation and subsequently minimize the formation of inhibitory compounds for microbial metabolism, limit the consumption of chemicals, energy and water, and the production of wastes [39]. The inhibitory compounds could be divided into four groups: (1) substances that are released by the hemicellulosic structure, such as acetic acid, which originates in the deacetylation of xylan; (2) phenolic compounds and other aromatic compounds derived from the partial degradation of lignin; (3) the furan derivatives, furfural and 5-hydroxymethylfurfural, resulting from the degradation of pentoses and hexoses, respectively; (4) metals like chromium, copper, iron, and nickel leached from the equipment [69]. These compounds individually as well as synergistically affect the physiology of fermenting microorganisms, therefore, it is essential to eliminate these inhibitory compounds or reduce their concentration to obtain the satisfactory product yields during microbial fermentation of lignocellulose hydrolysates [67].A number of methods like evaporation; neutralization; use of membranes, ion exchange resins, and activated charcoal; enzymatic detoxification using laccases and peroxidases have been attempted to detoxify the hydrolysates aiming ethanol production. Considering that different lignocellulosic hydrolysates have different degrees of inhibition and that microorganisms have different inhibitor tolerances, the methods of detoxification change will depend on the source of the lignocellulosic hydrolysate and the microorganism being used [87]. Several detoxification methods are described in this overview and can be divided into physical, physicochemical, chemical, and biological treatments.


### 5.3.1. Physical Treatments

(1) Evaporation (Concentration)
The concentration of hydrolysates by vacuum evaporation process is a physical detoxification method which reduces the volatile compounds concentration, including acetic acid, furfural and vanillin [87]. However, this treatment has the disadvantage of increasing the nonvolatile toxic compounds, as extractives [88].(2) Use of Membranes
The use of membranes has several advantages over conventional extraction. Membrane adsorption prevents that the aqueous phase (hydrolysate) is mixed with organic phase (solvent) which is likely to be toxic to microorganisms [89]. The membranes have surface functional groups attached to their internal pores, which may eliminate metabolic inhibitors as acetic acid, 5-hydroxymethylfurfural, furfural, formic, levulinic and sulphuric acid [67].


### 5.3.2. Physicochemical Treatments

(1) Ion Exchange Resins
The ion exchange resins process has been reported as the most efficient detoxification method. It is known that this process remove lignin-derived inhibitors, acetic acid, and furfurals of hydrolysate, improving significantly the yield fermentation [67]. The main advantage of the use of ion exchange resins is that they can be regenerated and reused without affecting the efficiency of the treatment [65, 90]. However, this method presents some disadvantages: the high pressure drop across the bed that tends to increase during operation due to media deformation; long processing time because of the slow pore diffusion; possible degradation of fragile biological product molecules, and it is difficult to scale-up [91]. The ion exchange resins process also leads to a significant loss of fermentable sugars after the process [67].(2) Neutralization
Considering the low pH of the hydrolysates provided by acid hydrolysis, it is needed that the neutralization of the pH to be close to the fermentations conditions. In this step, phenolics and furfurals are removed due to precipitations [67]. The chemicals employed in the neutralization of hydrolysates are calcium hydroxide and sodium hydroxide. The addition of Ca(OH)2 generates precipitate of CaSO4; therefore, it is desirable to be removed by centrifugation, adding one more stage in the process. During the generation of precipitates it can offer problems in fermentation [92].(3) Overliming
Among different types of detoxification, overliming is reported as the most used method [93]. This process consists in an increase of the pH of acid hydrolysate followed by reduction until a pH desirable to fermentations. The principle of this process is the precipitation of toxic components and the instability of some inhibitors at high pH [69]. This method showed high efficiency towards the removal of inhibitors and is being widely used [90, 94, 95]. This method has been considered promising and economic, revealing good efficiency for the removal of the furans compounds [67].(4) Activated Charcoal
Activated charcoal adsorption is a widely used detoxification method which is considered a low cost and efficient for inhibitors compounds removal. This method removes mainly phenolics compounds and does not provide large changes in the fermentable sugars levels [90]. The ratio of charcoal and hydrolysates, pH, time of contact, and temperature are the important factors for the improvement of this method [88, 96].(5) Extraction with Organic Solvents
Due to large availability of inhibitors such as acetic acid, furfural, vanillin, 4-hydroxybenzoic acid, and low molecular weight phenolics, the solvent extraction has been considered an efficient method of detoxification. The most common solvents used in this process are ethyl acetate, chloroform, and trichloroethylene [97].


### 5.3.3. Biological Treatment

Biological method uses specific enzymes or microorganisms that act on the inhibitors compounds present in the hydrolysate and change them [87]. In contrast with physical and chemical detoxifications, biological detoxification represents an improvement because little waste is generated and could be performed directly in the fermentation vessel before fermentation [98]. This method is still more feasible, environmental friendly, with fewer side reactions and less energy requirements [99]; however, it presents a long process time [100].The use of enzymes is a very studied and promising method. Laccase and peroxidase enzymes derived from white rot fungi have been found effective for the removal of phenolics compounds from lignocellulosic hydrolysates [99]. The detoxification mechanism of these enzymes probably involves oxidative polymerization of phenolic compounds of low molecular weight [88]; they catalyze the oxidation of substituted phenols, anilines, and aromatic thiols, at the expense of molecular oxygen [101]. The disadvantages of enzyme detoxification are long incubation time and high costs of enzymes; however it has the advantage that is usually conducted at mild conditions (pH 5.0, mesophilic temperature) [102].The use of microorganism has also been applied to remove inhibitors compounds from lignocellulosic hydrolysates [87]. There are several microorganisms which can naturally assimilate inhibitory compounds, including yeasts, fungi, and bacteria [102]. Some microorganisms during incubation are able to release cellulase and hemicellulase and degrade only lignin, resulting in a lignocellulosic substrate which can be easily hydrolyzed into fermentable sugars with mild conditions and short time [99].These microorganisms can effectively degrade lignin while retaining cellulose and hemicellulose in the substrate. This method can also be referredas in situ microbial delignification (ISMD). Recently, several microorganisms have shown their preference towards inhibitors by transforming their chemical nature and can be employed for detoxification of lignocellulose hydrolysates [99, 103]. The adaptation of a microorganism to a nondetoxified hydrolysate is another interesting alternative to replace the detoxification step. This method is based on successive fermentations using the microorganism of each experiment as the inoculum of the next one [88]. The use of adapted microorganisms not only reduces the detoxification cost but also avoids loss of fermentable sugars [102].


## 5.3.1. Physical Treatments

(1) Evaporation (Concentration)
The concentration of hydrolysates by vacuum evaporation process is a physical detoxification method which reduces the volatile compounds concentration, including acetic acid, furfural and vanillin [87]. However, this treatment has the disadvantage of increasing the nonvolatile toxic compounds, as extractives [88].(2) Use of Membranes
The use of membranes has several advantages over conventional extraction. Membrane adsorption prevents that the aqueous phase (hydrolysate) is mixed with organic phase (solvent) which is likely to be toxic to microorganisms [89]. The membranes have surface functional groups attached to their internal pores, which may eliminate metabolic inhibitors as acetic acid, 5-hydroxymethylfurfural, furfural, formic, levulinic and sulphuric acid [67].


## 5.3.2. Physicochemical Treatments

(1) Ion Exchange Resins
The ion exchange resins process has been reported as the most efficient detoxification method. It is known that this process remove lignin-derived inhibitors, acetic acid, and furfurals of hydrolysate, improving significantly the yield fermentation [67]. The main advantage of the use of ion exchange resins is that they can be regenerated and reused without affecting the efficiency of the treatment [65, 90]. However, this method presents some disadvantages: the high pressure drop across the bed that tends to increase during operation due to media deformation; long processing time because of the slow pore diffusion; possible degradation of fragile biological product molecules, and it is difficult to scale-up [91]. The ion exchange resins process also leads to a significant loss of fermentable sugars after the process [67].(2) Neutralization
Considering the low pH of the hydrolysates provided by acid hydrolysis, it is needed that the neutralization of the pH to be close to the fermentations conditions. In this step, phenolics and furfurals are removed due to precipitations [67]. The chemicals employed in the neutralization of hydrolysates are calcium hydroxide and sodium hydroxide. The addition of Ca(OH)2 generates precipitate of CaSO4; therefore, it is desirable to be removed by centrifugation, adding one more stage in the process. During the generation of precipitates it can offer problems in fermentation [92].(3) Overliming
Among different types of detoxification, overliming is reported as the most used method [93]. This process consists in an increase of the pH of acid hydrolysate followed by reduction until a pH desirable to fermentations. The principle of this process is the precipitation of toxic components and the instability of some inhibitors at high pH [69]. This method showed high efficiency towards the removal of inhibitors and is being widely used [90, 94, 95]. This method has been considered promising and economic, revealing good efficiency for the removal of the furans compounds [67].(4) Activated Charcoal
Activated charcoal adsorption is a widely used detoxification method which is considered a low cost and efficient for inhibitors compounds removal. This method removes mainly phenolics compounds and does not provide large changes in the fermentable sugars levels [90]. The ratio of charcoal and hydrolysates, pH, time of contact, and temperature are the important factors for the improvement of this method [88, 96].(5) Extraction with Organic Solvents
Due to large availability of inhibitors such as acetic acid, furfural, vanillin, 4-hydroxybenzoic acid, and low molecular weight phenolics, the solvent extraction has been considered an efficient method of detoxification. The most common solvents used in this process are ethyl acetate, chloroform, and trichloroethylene [97].


## 5.3.3. Biological Treatment

Biological method uses specific enzymes or microorganisms that act on the inhibitors compounds present in the hydrolysate and change them [87]. In contrast with physical and chemical detoxifications, biological detoxification represents an improvement because little waste is generated and could be performed directly in the fermentation vessel before fermentation [98]. This method is still more feasible, environmental friendly, with fewer side reactions and less energy requirements [99]; however, it presents a long process time [100].The use of enzymes is a very studied and promising method. Laccase and peroxidase enzymes derived from white rot fungi have been found effective for the removal of phenolics compounds from lignocellulosic hydrolysates [99]. The detoxification mechanism of these enzymes probably involves oxidative polymerization of phenolic compounds of low molecular weight [88]; they catalyze the oxidation of substituted phenols, anilines, and aromatic thiols, at the expense of molecular oxygen [101]. The disadvantages of enzyme detoxification are long incubation time and high costs of enzymes; however it has the advantage that is usually conducted at mild conditions (pH 5.0, mesophilic temperature) [102].The use of microorganism has also been applied to remove inhibitors compounds from lignocellulosic hydrolysates [87]. There are several microorganisms which can naturally assimilate inhibitory compounds, including yeasts, fungi, and bacteria [102]. Some microorganisms during incubation are able to release cellulase and hemicellulase and degrade only lignin, resulting in a lignocellulosic substrate which can be easily hydrolyzed into fermentable sugars with mild conditions and short time [99].These microorganisms can effectively degrade lignin while retaining cellulose and hemicellulose in the substrate. This method can also be referredas in situ microbial delignification (ISMD). Recently, several microorganisms have shown their preference towards inhibitors by transforming their chemical nature and can be employed for detoxification of lignocellulose hydrolysates [99, 103]. The adaptation of a microorganism to a nondetoxified hydrolysate is another interesting alternative to replace the detoxification step. This method is based on successive fermentations using the microorganism of each experiment as the inoculum of the next one [88]. The use of adapted microorganisms not only reduces the detoxification cost but also avoids loss of fermentable sugars [102].


## 5.4. Fermentation of Sugars from Sugarcane Biomass into Ethanol

### 5.4.1.  Bioconversion of Hexose Sugars into Ethanol

Ethanol fermentation is a biological process in which sugars are converted by microorganisms to produce ethanol and CO2. Even though there are the existence of many methods and process to use lignocellulosic materials for ethanol production, however, it is still difficult to obtain economic ethanol from lignocellulosics [86].The microorganism most commonly used in fermentation process is the yeasts and, among the yeasts,Saccharomyces cerevisiae is the preferred choice for ethanol fermentation [104]. This yeast can grow both on simple sugars, such as glucose, and on the disaccharide sucrose. Furthermore, the availability of a robust genetic transformation system of S. cerevisiae along with a long history of this microorganism in industrial fermentation processes makes it most desired microorganisms for ethanol production. S. cerevisiae has high resistance to ethanol, consumes significant amounts of substrate in adverse conditions, and shows high resistance to inhibitors present in the medium [105]. Unfortunately, xylose metabolism presents a unique challenge for S. cerevisiae to assimilate pentose sugars due to the absence of genes required for assimilation of these molecules [105].There are three kinds of processes to produce ethanol from sugarcane bagasse (SB) and sugarcane straw (SS). The first process is called separate (or sequential) hydrolysis and fermentation (SHF) where hydrolysis of lignocellulosic material and ethanol fermentation is done separately. SB/SS is pretreated, and the pretreated material is enzymatically hydrolyzed separately to recover the sugars. The recovered sugar solution (hexose sugars) is then fermented with appropriate microorganism into ethanol. SHF is a little staggered process [21]. The other two kinds of processes are called simultaneous saccharification and fermentation (SSF) and simultaneous saccharification and cofermentation (SSCF), where both enzymatic hydrolysis and fermentation of released sugars into ethanol occur simultaneously making the overall process short [21, 86]. In the SSF process, the glucose (from cellulose hydrolyzed) is fermented separately of pentoses (from hydrolysate) in a separate reactor, while that in the SSCF process, the fermentation of xylose and glucose occurs together in the same reactor [21, 86].


### 5.4.2. Bioconversion of Pentose Sugars into Ethanol

The maximum utilization of all sugar fractions is essential to obtain an economic and viable conversion technology for bioethanol production from sugarcane bagasse (SB) and sugarcane straw (SS). To obtain the desired ethanol yields from SB/SS hydrolysates, it is essential that the hemicellulose fraction should be fermented with same conversion rates as the cellulose fraction [104].Hemicellulose hydrolysate typically contains primarily pentose sugars (xylose and arabinose) and some amounts of hexose sugars (mannose, glucose and galactose) [106, 107]. A variety of yeast, fungi, and bacteria are capable of assimilating pentose, but only a few are promising candidates for the efficient xylose fermentation into ethanol [106]. In yeasts, the assimilation of D-xylose follows the pathway where the sugar passes through a pool enzymatic to enter in the phosphopentose pathway [108]. There are several microorganisms capable of assimilating pentose sugars, but only few species are capable of assimilating sugars to produce ethanol at industrial scale. Microorganisms, such as Scheffersomyces stipitis (Pichia stipitis) [109], Candida guilliermondii, Candida shehatae, and Pachysolen tannophilus are able to assimilate pentose sugars by a reduction/oxidation pathway bioconversion of sugarcane bagasse/sugarcane straw hydrolysates under different cultivation conditions [110].The process to assimilate pentose sugars consists in the xylose being converted to xylitol, by the action of D-xylose reductase (E.C. 1.1.1.21) and immediately oxidized by the action of xylitol dehydrogenase (E.C. 1.1.1.9), producing D-xylose-5-phosphate. Ribolosephosphate-3-epimerase (5.1.3.1), transaldolase (E.C. 2.2.1.2), and transketolase (E.C. 2.2.1.1) sequentially convert D-xylose-5-phosphate into glyceraldehyde-3-phosphate and fructose-6-phoshate by non-oxidative rearrangement resulting into the formation of ethanol by the Emden-Meyorhoff Pathway. NADPH must be regenerated through metabolic routes. The metabolic pathway of the arabinose is similar to the route shown by xylose, where aldose reductase mechanistically converts arabinose into L-arabitol. Through the action of L-arabitol dehydrogenase, L-arabitol is reduced to ethanol [99, 105]. Recently published reviews have competently presented the important progress made for pentose sugars fermentation into ethanol [99, 110].


## 5.4.1.  Bioconversion of Hexose Sugars into Ethanol

Ethanol fermentation is a biological process in which sugars are converted by microorganisms to produce ethanol and CO2. Even though there are the existence of many methods and process to use lignocellulosic materials for ethanol production, however, it is still difficult to obtain economic ethanol from lignocellulosics [86].The microorganism most commonly used in fermentation process is the yeasts and, among the yeasts,Saccharomyces cerevisiae is the preferred choice for ethanol fermentation [104]. This yeast can grow both on simple sugars, such as glucose, and on the disaccharide sucrose. Furthermore, the availability of a robust genetic transformation system of S. cerevisiae along with a long history of this microorganism in industrial fermentation processes makes it most desired microorganisms for ethanol production. S. cerevisiae has high resistance to ethanol, consumes significant amounts of substrate in adverse conditions, and shows high resistance to inhibitors present in the medium [105]. Unfortunately, xylose metabolism presents a unique challenge for S. cerevisiae to assimilate pentose sugars due to the absence of genes required for assimilation of these molecules [105].There are three kinds of processes to produce ethanol from sugarcane bagasse (SB) and sugarcane straw (SS). The first process is called separate (or sequential) hydrolysis and fermentation (SHF) where hydrolysis of lignocellulosic material and ethanol fermentation is done separately. SB/SS is pretreated, and the pretreated material is enzymatically hydrolyzed separately to recover the sugars. The recovered sugar solution (hexose sugars) is then fermented with appropriate microorganism into ethanol. SHF is a little staggered process [21]. The other two kinds of processes are called simultaneous saccharification and fermentation (SSF) and simultaneous saccharification and cofermentation (SSCF), where both enzymatic hydrolysis and fermentation of released sugars into ethanol occur simultaneously making the overall process short [21, 86]. In the SSF process, the glucose (from cellulose hydrolyzed) is fermented separately of pentoses (from hydrolysate) in a separate reactor, while that in the SSCF process, the fermentation of xylose and glucose occurs together in the same reactor [21, 86].


## 5.4.2. Bioconversion of Pentose Sugars into Ethanol

The maximum utilization of all sugar fractions is essential to obtain an economic and viable conversion technology for bioethanol production from sugarcane bagasse (SB) and sugarcane straw (SS). To obtain the desired ethanol yields from SB/SS hydrolysates, it is essential that the hemicellulose fraction should be fermented with same conversion rates as the cellulose fraction [104].Hemicellulose hydrolysate typically contains primarily pentose sugars (xylose and arabinose) and some amounts of hexose sugars (mannose, glucose and galactose) [106, 107]. A variety of yeast, fungi, and bacteria are capable of assimilating pentose, but only a few are promising candidates for the efficient xylose fermentation into ethanol [106]. In yeasts, the assimilation of D-xylose follows the pathway where the sugar passes through a pool enzymatic to enter in the phosphopentose pathway [108]. There are several microorganisms capable of assimilating pentose sugars, but only few species are capable of assimilating sugars to produce ethanol at industrial scale. Microorganisms, such as Scheffersomyces stipitis (Pichia stipitis) [109], Candida guilliermondii, Candida shehatae, and Pachysolen tannophilus are able to assimilate pentose sugars by a reduction/oxidation pathway bioconversion of sugarcane bagasse/sugarcane straw hydrolysates under different cultivation conditions [110].The process to assimilate pentose sugars consists in the xylose being converted to xylitol, by the action of D-xylose reductase (E.C. 1.1.1.21) and immediately oxidized by the action of xylitol dehydrogenase (E.C. 1.1.1.9), producing D-xylose-5-phosphate. Ribolosephosphate-3-epimerase (5.1.3.1), transaldolase (E.C. 2.2.1.2), and transketolase (E.C. 2.2.1.1) sequentially convert D-xylose-5-phosphate into glyceraldehyde-3-phosphate and fructose-6-phoshate by non-oxidative rearrangement resulting into the formation of ethanol by the Emden-Meyorhoff Pathway. NADPH must be regenerated through metabolic routes. The metabolic pathway of the arabinose is similar to the route shown by xylose, where aldose reductase mechanistically converts arabinose into L-arabitol. Through the action of L-arabitol dehydrogenase, L-arabitol is reduced to ethanol [99, 105]. Recently published reviews have competently presented the important progress made for pentose sugars fermentation into ethanol [99, 110].


## 5.5. Distillation of Ethanol

Despite the downstream process being the highest energy consuming process during the ethanol production [111], the ethanol recovery from the fermented broth is necessary. The final medium is composed by water and ethanol (5–12 wt%) [112]. The ethanol-water cannot be separated by conventional distillation processes because they form a nonideal mixture system [113]. The dehydration is sophisticated method because they form an azeotropic mixture with water (at 95.6 wt% at a temperature of 78.15°C), which makes it impossible to separate in a single distillation column [112]. This way, the ethanol purification occurs in three steps: distillation, rectification, and dehydration. A high concentrated ethanol solution is obtained in the two first steps (about 92.4 wt%), then the mixture is dehydrated in order to obtain ethanol anhydrous by a dehydration method. The dehydration can be realized by azeotropic distillation, extractive distillation, liquid-liquid extraction, adsorption, or some complex hybrid separation methods [112].


## 6. Conclusion

Sugarcane bagasse (SB) and sugarcane straw (SS) are the attractive second-generation renewable feedstock available in several countries like Brazil. This feedstock if used judiciously may provide the sustainable supply of drop-in ethanol, industrial enzymes, organics acids, single cell proteins, and so forth. However, a significant fraction of this biomass goes to industries for steam and electricity generation. The remaining fraction represents the ideal feedstock for the generation high-value commodities. Last three decades of vigorous developments in pretreatment technologies, microbial biotechnology, and downstream processing have made it reality to harness the sugarcane residues for the production of many products of commercial significance at large scale without jeopardizing the food/feed requirements. Biomass recalcitrance is a main challenge toward the successful exploitation of these residues. To overcome the biomass recalcitrance, pretreatment is an inevitable process to ameliorate the accessibility of carbohydrate for the subsequent enzymatic hydrolysis reaction to generate fermentable sugars. There are several robust pretreatment methods available; however, the ultimate choice for the selection of pretreatment process depends upon the effective delignification or hemicellulose removal, minimum generation of inhibitors, low sugar loss, time savings, being economic and causing less environmental pollution. The released sugars after enzymatic hydrolysis and hemicellulose depolymerization are converted into ethanol by the suitable ethnologic strain. In order to get desired ethanol yields, the ethnologic strains should have ability to utilize pentose and hexose sugars, inhibitor resistance, and high osmotolerance. The following ten requirements are pivotal in order to establish a long-term sustainable second-generation ethanol production process from sugarcane residues.(1)
Fullest utilization of SB and SS generated in the country for the better management.(2)
Selection of right pretreatment and detoxification strategy.(3)
In-house cellulase production and development of cellulolytic strains and ethanol producing strains from pentose and hexose sugars showing inhibitor resistance, ethanol tolerance, and faster sugar conversion rates.(4)
Process intensification: hydrolysis and fermentation together in one place.(5)
Cheap, fast, and effective ethanol distillation.(6)
Integration of bioethanol producing units with sugar/distilleries for the coutilization of machinery, reactors, and other equipment.(7)
Maximum by products utilization (lignin, furans, and yeast cell mass).(8)
Environmental protection.(9)
Government subsidies to promote the renewable energy.(10)
Encouragement of private investments.



---

*Source: 989572-2012-11-26.xml*