# Antiobesity and Hypoglycaemic Effects of Aqueous Extract ofIbervillea sonorae in Mice Fed a High-Fat Diet with Fructose

Authors: Fabiola Rivera-Ramírez; Gerardo N. Escalona-Cardoso; Leticia Garduño-Siciliano; Carlos Galaviz-Hernández; Norma Paniagua-Castro
Journal: Journal of Biomedicine and Biotechnology
 (2011)

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## Abstract

Obesity, type II diabetes, and hyperlipidaemia, which frequently coexist and are strongly associated with oxidative stress, increase the risk of cardiovascular disease. An increase in carbohydrate intake, especially of fructose, and a high-fat diet are both factors that contribute to the development of these metabolic disorders. In recent studies carried out in diabetic rats, authors reported thatIbervillea sonorae had hypoglycaemic activity; saponins and monoglycerides present in the plant could be responsible for the effects observed. In the present study, we determined the effects of an aqueous I. sonorae extract on a murine model of obesity and hyperglycaemia, induced by a high-calorie diet, and the relationship of these effects with hepatic oxidation. A high-fat diet over a period of 8 weeks induced weight gain in the mice and increased triglycerides and blood glucose levels. Simultaneous treatment with I. sonorae aqueous extracts, at doses of 100, 200, and 400 mg/kg, decreased triglycerides and glycaemia levels, prevented an increase in body weight in a dose-dependent manner, and decreased hepatic lipid oxidation at a dose of 200 mg/kg. These data suggest that the aqueous extract from I. sonorae root prevents obesity, dyslipidaemia, and hyperglycaemia induced by a hypercaloric diet; however, high doses may induce toxicity.


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## Body

## 1. Introduction

Obesity is a global health problem and is associated with the development of chronic diseases such as hyperlipidaemias, type II diabetes, and cardiovascular complications. Oxidative stress is linked to obesity, and adipose tissue can produce adipokines that induce production of reactive oxygen species (ROS) that cause insulin resistance. All of these factors may contribute to metabolic syndrome [1]. It is estimated that genetic factors, high-fat diets, and sedentary lifestyles are the main factors underlying the development of these diseases [2, 3]. In Mexico, as in other countries, a rise in the number of obesity cases in adults and children has been noted [4–6]. Other reports have demonstrated that high consumption of refined carbohydrates, especially fructose, can induce obesity and hyperglycaemia, with concomitant insulin resistance [7, 8]. The primary recommendations to manage the disease include diet modification and physical exercise to achieve weight loss [9], followed by pharmacological treatment.A variety of natural products have been proposed as pharmacological treatments for obesity. The products that can induce body weight reduction and prevent diet-induced obesity in animal models include crude extracts and compounds isolated from plants [10]. Therefore, they have also been recommended as an aid in prevention of obesity. Other natural products such as cinnamon [11], green tea [12], and oats [13] have demonstrated antioxidant, hypoglycaemic, and hypolipidaemic properties. The use and acceptance of traditional medicine have increased in recent years [14, 15]. In traditional Mexican medicine the root of Ibervillea sonorae(IS), colloquially known as “wareke,” is widely used as a topical antibiotic, cathartic, antirheumatic [16], and, recently, hypoglycaemic [17]. Previously published pharmacological properties for IS root include antiviral and antifungal [18], cytotoxic [19], and hypoglycaemic effects.  Alarcon-Aguilar et al. [20] demonstrated the hypoglycaemic action of dichloromethane-extracted IS on healthy and aloxane-induced diabetic mice [21]; however, its hypolipidaemic effects remain unclear. The present study evaluated the effect of the aqueous extract of IS on glucose and lipid levels as well as hepatic oxidation in mice fed a high-fat diet plus fructose.


## 2. Materials and Methods

### 2.1. Plant Material

The dried powder ofIbervillea sonorae (IS) root was purchased from Nutra Herbal (Mexico City) (http://www.nutraherbalmexico.com/). IS samples were water extracted at 90°C with 10 g sample per 100 mL water and allowed to cool at room temperature. The extract was then filtered and freeze-dried (Freeze Dry System/Freezone 4.5, LABCONCO, USA). The extraction yield for the resulting brown powder was 8%, and it was stored at −20°C until use. Doses of 100, 200, and 400 mg/kg lyophilised extract were dissolved in distilled water and administered daily by gavage.


### 2.2. High-Fat Diet

The high-fat diet was prepared by adding 35% lard to a ground standard diet. Fructose (Cedrosa SA Mexico) solution (60%) was administered daily by gavage. Food intake was measured every day, and body weight was measured once every two days. The composition of the diets is shown in Table1.Table 1
Composition of the control and test diets.
Control (%)*HF (%)**Protein28.0518.23Fat12.147.89Lard035.00Carbohydrate59.8138.88Total (g)100100Calories (kcal)467.5614.5*Lab Chow 5012 diet.**Fructose (60%) was administered by gavage (2.4 kcal).


### 2.3. Animals

Male ICR mice (Biorepository of the National School of Biological Sciences) weighing 25–30 g were housed in a room at a constant temperature of 22 ± 1°C and a 12 h light/dark cycle (lights on at 9:00 AM) and were given food and waterad libitum. All experiments were approved by the Laboratory Animal Care Committee of the National School of Biological Sciences (National Polytechnic Institute) and were conducted in compliance with the Mexican Official Standard (NOM—062-200-1999) technical specifications for the production, care, and use of laboratory animals.The animals were distributed into 6 groups of 10 animals each and were fed a high-fat diet plus fructose (HFF). Mice receiving the aqueous IS extract were dosed for 8 weeks in parallel, as detailed in Table2.Table 2
Treatment groups.
GroupsTreatment (mg/kg body weight)1Control2High fat-fructose (HFF)3Ibervillea sonorae (IS) 4004HFF + IS 1005HFF + IS 2006HFF + IS 400


### 2.4. Biochemical Parameters

After eight weeks of treatment, the animals were fasted for 8 hours and then sacrificed. Blood was collected by retroorbital puncture, and serum was prepared by centrifuging the blood at 3000 rpm for 20 minutes. The levels of serum glucose, total cholesterol (TC), HDL cholesterol (HDL-C), and triglycerides (TGs) were determined to analyse using the automatic Selectra analyser (Modelo Vitalab 2), with commercial kits (RANDOX). The Friedewald formula [22] was used to calculate serum low-density lipoprotein cholesterol (LDL-C) values and atherogenic index as follows: LDL-C = TC-(HDL-C + TG/5); atherogenic index = (TC-HDL-C)/HDL-C. The liver and periepididymal fat were also weighed.


### 2.5. Hepatic Lipid Peroxidation Assay

The hepatic weight was recorded and lipid peroxidation assessed by measuring malondialdehyde (MDA) content as described by Buege and Aust [23], with minor modifications. Briefly, 50 mg liver was homogenised in 0.5 mL cold phosphate buffer (pH 7.0), followed immediately with the addition of 1 mL of a reagent containing trichloroacetic acid (TCA), thiobarbituric acid (TBA) and HCl (15% (w/v), 0.375% (w/v), and 0.25 N, resp.) to the homogenate. The solution was then heated for 30 min in a boiling water bath. After cooling, the flocculent precipitate was removed by centrifugation at 1000 g for 10 min. A Bio-Mini Shimadzu Spectrophotometer was used to determine absorbance at 532 nm. A sample containing reagent only was used as a blank. The MDA content was calculated using an extinction coefficient of 1.56 × 10−5 M−1cm−1. The protein content was determined using the Coomassie blue method [24], with bovine serum albumin (BSA) as the standard.


### 2.6. Statistical Analysis

Data were analysed with one-way ANOVAs followed by Holm-Sidak post hoc tests, with significance set toP<0.05. Food consumption was analysed using two-way ANOVA repeated measures tests followed by Tukey’s post hoc tests, with significance set to P<0.05. All analyses were performed using Sigma Stat (10.0).


## 2.1. Plant Material

The dried powder ofIbervillea sonorae (IS) root was purchased from Nutra Herbal (Mexico City) (http://www.nutraherbalmexico.com/). IS samples were water extracted at 90°C with 10 g sample per 100 mL water and allowed to cool at room temperature. The extract was then filtered and freeze-dried (Freeze Dry System/Freezone 4.5, LABCONCO, USA). The extraction yield for the resulting brown powder was 8%, and it was stored at −20°C until use. Doses of 100, 200, and 400 mg/kg lyophilised extract were dissolved in distilled water and administered daily by gavage.


## 2.2. High-Fat Diet

The high-fat diet was prepared by adding 35% lard to a ground standard diet. Fructose (Cedrosa SA Mexico) solution (60%) was administered daily by gavage. Food intake was measured every day, and body weight was measured once every two days. The composition of the diets is shown in Table1.Table 1
Composition of the control and test diets.
Control (%)*HF (%)**Protein28.0518.23Fat12.147.89Lard035.00Carbohydrate59.8138.88Total (g)100100Calories (kcal)467.5614.5*Lab Chow 5012 diet.**Fructose (60%) was administered by gavage (2.4 kcal).


## 2.3. Animals

Male ICR mice (Biorepository of the National School of Biological Sciences) weighing 25–30 g were housed in a room at a constant temperature of 22 ± 1°C and a 12 h light/dark cycle (lights on at 9:00 AM) and were given food and waterad libitum. All experiments were approved by the Laboratory Animal Care Committee of the National School of Biological Sciences (National Polytechnic Institute) and were conducted in compliance with the Mexican Official Standard (NOM—062-200-1999) technical specifications for the production, care, and use of laboratory animals.The animals were distributed into 6 groups of 10 animals each and were fed a high-fat diet plus fructose (HFF). Mice receiving the aqueous IS extract were dosed for 8 weeks in parallel, as detailed in Table2.Table 2
Treatment groups.
GroupsTreatment (mg/kg body weight)1Control2High fat-fructose (HFF)3Ibervillea sonorae (IS) 4004HFF + IS 1005HFF + IS 2006HFF + IS 400


## 2.4. Biochemical Parameters

After eight weeks of treatment, the animals were fasted for 8 hours and then sacrificed. Blood was collected by retroorbital puncture, and serum was prepared by centrifuging the blood at 3000 rpm for 20 minutes. The levels of serum glucose, total cholesterol (TC), HDL cholesterol (HDL-C), and triglycerides (TGs) were determined to analyse using the automatic Selectra analyser (Modelo Vitalab 2), with commercial kits (RANDOX). The Friedewald formula [22] was used to calculate serum low-density lipoprotein cholesterol (LDL-C) values and atherogenic index as follows: LDL-C = TC-(HDL-C + TG/5); atherogenic index = (TC-HDL-C)/HDL-C. The liver and periepididymal fat were also weighed.


## 2.5. Hepatic Lipid Peroxidation Assay

The hepatic weight was recorded and lipid peroxidation assessed by measuring malondialdehyde (MDA) content as described by Buege and Aust [23], with minor modifications. Briefly, 50 mg liver was homogenised in 0.5 mL cold phosphate buffer (pH 7.0), followed immediately with the addition of 1 mL of a reagent containing trichloroacetic acid (TCA), thiobarbituric acid (TBA) and HCl (15% (w/v), 0.375% (w/v), and 0.25 N, resp.) to the homogenate. The solution was then heated for 30 min in a boiling water bath. After cooling, the flocculent precipitate was removed by centrifugation at 1000 g for 10 min. A Bio-Mini Shimadzu Spectrophotometer was used to determine absorbance at 532 nm. A sample containing reagent only was used as a blank. The MDA content was calculated using an extinction coefficient of 1.56 × 10−5 M−1cm−1. The protein content was determined using the Coomassie blue method [24], with bovine serum albumin (BSA) as the standard.


## 2.6. Statistical Analysis

Data were analysed with one-way ANOVAs followed by Holm-Sidak post hoc tests, with significance set toP<0.05. Food consumption was analysed using two-way ANOVA repeated measures tests followed by Tukey’s post hoc tests, with significance set to P<0.05. All analyses were performed using Sigma Stat (10.0).


## 3. Results

### 3.1. Body Weight

The effect of a high-fat diet plus fructose (HFF) on the body weight of mice is shown in Figure1. During the 8 weeks of treatment, the weight of the HFF group increased significantly from the first week until the end of treatment when compared with the control group. Mice receiving IS only (400 mg/kg) underwent a 6% loss in weight relative to the starting weight. All doses of IS (100, 200, and 400 mg/kg) prevented weight gain in mice fed HFF.Figure 1
Body weight of mice fed a high-fat diet plus fructose (HFF) and treated withI. sonorae(IS) for 8 weeks; a: significant difference compared to control; b: significant difference compared to HFF, P<0.001 (one-way ANOVA, Holm-Sidak post hoc test, n=10 per group).


### 3.2. Food Intake

Throughout treatment, the mice that received the HFF diet consumed less food than the control group, but their calorie intake was higher (Figures2(a) and 2(b)). Coadministration of the aqueous extract of IS reduced food intake in all groups, with a greater effect in groups treated with 200 and 400 mg/kg (Figure 2).Food and calorie consumption (kcal) in mice fed a high-fat diet plus fructose (HFF) and treated withI. sonorae(IS) for 8 weeks; a: significant difference compared to control; b: significant difference compared to HFF, P<0.05 (one-way ANOVA, Tukey’s post hoc test).
(a)(b)Compared with control-fed mice, the amount of periepididymal fat was higher in mice fed the HFF diet. All three doses of IS extract resulted in significantly reduced fat deposition in this region (Figure3).Figure 3
Periepididymal fat in mice fed control diet, or HFF and/or IS for 8 weeks; a: significant difference compared to control; b: significant difference compared to HFF,P<0.05 (one-way ANOVA, Tukey’s post hoc test).


### 3.3. Biochemical Parameters

The HFF diet increased triglyceride levels 1.9-fold (from 0.88 to 1.7 mmol/L), total cholesterol 1.8-fold (from 18.6 to 35.2 mmol/L), HDL-C 2.1-fold (from 9.5 to 20.8 mmol/L), LDL-C 1.2-fold (from 11 to 13.4 mmol/L), and glucose levels from 84.4 to 169.8 mg/dL. Both triglycerides and blood glucose levels in mice fed the HFF diet were significantly different from mice fed with control diet (P<0.05, Table 3).Table 3
Glycaemia and lipid profile in male mice fed HFF and/or IS for 8 weeks.
ControlIS 400 mg/kgHFFHFF + IS 100 mg/kgHFF + IS  200 mg/kgHFF + IS  400 mg/kgTriglycerides (mmol/L)0.9 ± 0.051.15 ± 0.2a1.7 ± 0.2a1.36 ± 0.21.2 ± 0.110.96 ± 0.11bCholesterol (mmol/L)18.6 ± 0.429 ± 2.2a35 ± 2.8a38.8 ± 3.8a32.3 ± 3.8a28.4 ± 1.4bHDL-C (mmol/L)9.5 ± 0.67.7 ± 1.6b20.8 ± 1.4a16.4 ± 1.7a16.1 ± 0.5a16.4 ± 1.4aLDL-C (mmol/L)12.1 ± 1.721.8 ± 6.1a15.0 ± 3.223.1 ± 4.5a16.7 ± 0.0112.40 ± 1.4Glycaemia (mg/dL)84.4 ± 6.796 ± 7.1169.8 ± 13.9a141 ± 21.4a128.3 ± 13.2a91.8 ± 7.1bAtherogenic  index0.9 ± 0.22.8 ± 1.1a0.7 ± 0.91.4 ± 0.71.0 ± 0.090.7 ± 1.2Data are mean ± S.E. HFF: high fat and fructose; IS:Ibervillea sonorae; a: significant difference compared to Control; b: significant difference compared to HFF, P<0.05 (one-way ANOVA, Tukey’s post hoc test).The 400 mg/kg dose of IS increased cholesterol and LDL levels when administered alone. However, in HFF-fed mice, triglyceride and glucose levels were reduced in a dose-dependent manner by IS, with the highest dose inducing a significant reduction in these parameters. In control-fed mice, IS (400 mg/kg) increased the lipid profile and atherogenic index.


### 3.4. Hepatic Lipid Peroxidation

No significant differences were found in liver weight of mice fed the HFF diet and/or treated with IS (Figure4(a)). However, the group receiving the HFF diet had higher levels of hepatic lipid peroxidation than control-fed mice (Figure 4(b)). Co-administration of 200 mg/kg of IS decreased oxidation levels; however, the lower (100 mg/kg) and higher (400 mg/kg) doses showed no liver protection.(a) Weight and (b) lipid peroxidation levels in liver of mice fed a high-fat diet plus fructose (HFF) and treated withI. sonorae(IS) for 8 weeks; a: significant difference compared to Control; b: significant difference compared to HFF, P<0.05 (one way ANOVA, Tukey’s post hoc test).
(a)(b)


## 3.1. Body Weight

The effect of a high-fat diet plus fructose (HFF) on the body weight of mice is shown in Figure1. During the 8 weeks of treatment, the weight of the HFF group increased significantly from the first week until the end of treatment when compared with the control group. Mice receiving IS only (400 mg/kg) underwent a 6% loss in weight relative to the starting weight. All doses of IS (100, 200, and 400 mg/kg) prevented weight gain in mice fed HFF.Figure 1
Body weight of mice fed a high-fat diet plus fructose (HFF) and treated withI. sonorae(IS) for 8 weeks; a: significant difference compared to control; b: significant difference compared to HFF, P<0.001 (one-way ANOVA, Holm-Sidak post hoc test, n=10 per group).


## 3.2. Food Intake

Throughout treatment, the mice that received the HFF diet consumed less food than the control group, but their calorie intake was higher (Figures2(a) and 2(b)). Coadministration of the aqueous extract of IS reduced food intake in all groups, with a greater effect in groups treated with 200 and 400 mg/kg (Figure 2).Food and calorie consumption (kcal) in mice fed a high-fat diet plus fructose (HFF) and treated withI. sonorae(IS) for 8 weeks; a: significant difference compared to control; b: significant difference compared to HFF, P<0.05 (one-way ANOVA, Tukey’s post hoc test).
(a)(b)Compared with control-fed mice, the amount of periepididymal fat was higher in mice fed the HFF diet. All three doses of IS extract resulted in significantly reduced fat deposition in this region (Figure3).Figure 3
Periepididymal fat in mice fed control diet, or HFF and/or IS for 8 weeks; a: significant difference compared to control; b: significant difference compared to HFF,P<0.05 (one-way ANOVA, Tukey’s post hoc test).


## 3.3. Biochemical Parameters

The HFF diet increased triglyceride levels 1.9-fold (from 0.88 to 1.7 mmol/L), total cholesterol 1.8-fold (from 18.6 to 35.2 mmol/L), HDL-C 2.1-fold (from 9.5 to 20.8 mmol/L), LDL-C 1.2-fold (from 11 to 13.4 mmol/L), and glucose levels from 84.4 to 169.8 mg/dL. Both triglycerides and blood glucose levels in mice fed the HFF diet were significantly different from mice fed with control diet (P<0.05, Table 3).Table 3
Glycaemia and lipid profile in male mice fed HFF and/or IS for 8 weeks.
ControlIS 400 mg/kgHFFHFF + IS 100 mg/kgHFF + IS  200 mg/kgHFF + IS  400 mg/kgTriglycerides (mmol/L)0.9 ± 0.051.15 ± 0.2a1.7 ± 0.2a1.36 ± 0.21.2 ± 0.110.96 ± 0.11bCholesterol (mmol/L)18.6 ± 0.429 ± 2.2a35 ± 2.8a38.8 ± 3.8a32.3 ± 3.8a28.4 ± 1.4bHDL-C (mmol/L)9.5 ± 0.67.7 ± 1.6b20.8 ± 1.4a16.4 ± 1.7a16.1 ± 0.5a16.4 ± 1.4aLDL-C (mmol/L)12.1 ± 1.721.8 ± 6.1a15.0 ± 3.223.1 ± 4.5a16.7 ± 0.0112.40 ± 1.4Glycaemia (mg/dL)84.4 ± 6.796 ± 7.1169.8 ± 13.9a141 ± 21.4a128.3 ± 13.2a91.8 ± 7.1bAtherogenic  index0.9 ± 0.22.8 ± 1.1a0.7 ± 0.91.4 ± 0.71.0 ± 0.090.7 ± 1.2Data are mean ± S.E. HFF: high fat and fructose; IS:Ibervillea sonorae; a: significant difference compared to Control; b: significant difference compared to HFF, P<0.05 (one-way ANOVA, Tukey’s post hoc test).The 400 mg/kg dose of IS increased cholesterol and LDL levels when administered alone. However, in HFF-fed mice, triglyceride and glucose levels were reduced in a dose-dependent manner by IS, with the highest dose inducing a significant reduction in these parameters. In control-fed mice, IS (400 mg/kg) increased the lipid profile and atherogenic index.


## 3.4. Hepatic Lipid Peroxidation

No significant differences were found in liver weight of mice fed the HFF diet and/or treated with IS (Figure4(a)). However, the group receiving the HFF diet had higher levels of hepatic lipid peroxidation than control-fed mice (Figure 4(b)). Co-administration of 200 mg/kg of IS decreased oxidation levels; however, the lower (100 mg/kg) and higher (400 mg/kg) doses showed no liver protection.(a) Weight and (b) lipid peroxidation levels in liver of mice fed a high-fat diet plus fructose (HFF) and treated withI. sonorae(IS) for 8 weeks; a: significant difference compared to Control; b: significant difference compared to HFF, P<0.05 (one way ANOVA, Tukey’s post hoc test).
(a)(b)


## 4. Discussion

Fructose is a sweetener in sugary drinks, and its consumption has increased in recent years. Sugar metabolism occurs primarily in the liver, where a high fructose flux leads to enhanced accumulation of hepatic triglycerides, resulting in impaired glucose and lipid metabolism and increased cardiovascular risk [25–27]. Diets high in fat also result in increased levels of blood lipids and glucose; therefore, it is proposed that an overexpression of hepatic 1–6 bisphosphatase, which is involved in gluconeogenesis, may be responsible for hyperglycaemia in type II diabetes [28]. Observed increases in body weight after consumption of a high-fat diet are mainly due to the hyperplasticity and hypertrophy of adipose tissue [29]. Furthermore, studies in humans and in animal models suggest that dietary components such as high fat and fructose can affect fatty infiltration and lipid peroxidation in various diseases of the liver [30, 31]. Extensive interventions are recommended when treating obesity and hyperglycaemia, including diet control, exercise, and pharmacological interventions. However, many patients do not respond to treatment and may present with adverse treatment effects, indicating a need for efficacious alternatives. Some plants contain active substances with important pharmacological activities, and they represent an excellent alternative. In the murine model used in this study, we found the expected HFF-induced increase in body weight and periepididymal fat as well as hyperglycaemia and hypertriglyceridemia. IS treatment prevented these alterations, suggesting that the aqueous extract of IS exhibits pharmacological antiobesity effects. Our results show that the HFF diet increased body weight in mice, even though food intake was lower compared with the control diet. The HFF diet provides more calories than a normal diet, resulting in a high level of fat storage in the periepididymal region. IS administration prevented weight gain and reduced the consumption of food and calories in HFF-fed mice. However, 400 mg/kg IS aqueous extract alone reduced food intake and caused weight loss in mice. This could be a toxic effect in normal mice fed a normal diet, but this effect had not been reported before.The HFF diet increased cholesterol, triglycerides, and blood glucose levels, as well as HDL-C, suggesting that the HFF diet is not atherogenic. Co-administration of IS extract, at 400 mg/kg, reduced triglycerides, cholesterol, and glycaemia levels, but lower doses did not show a similar activity. IS aqueous extract alone (400 mg/kg) increased triglycerides, cholesterol, and LDL-C but decreased HDL-C, which suggests an atherogenic effect in normal mice; a result not reported previously. Andrade-Cetto and Heinrich [18] and Alarcon-Aguilar et al. [32]  reported that aqueous and dichloromethane extracted IS reduced blood glucose levels significantly in mice with alloxan-induced diabetes. The authors suggested that the hypoglycaemic effect may have been due to an improvement in insulin sensitivity and/or reduced insulin resistance. However, functional pancreatic β cells were required to achieve these effects, which suggests a potential therapeutic use of IS for type II diabetes. As observed in results from the liver TBARS assay, our data showed increased hepatic lipid peroxidation in the HFF-fed group. These results indicated that eight weeks of HFF diet induced oxidative stress that could damage the liver. IS extract dosed at 200 mg/kg reduced hepatic oxidation in HFF-fed mice, while lower and higher doses had no effect.The antiobesity effects of IS have not been reported previously. Although the mechanism of action of IS is not clearly established, it has been proposed to result from constituents such as saponins and flavonoids [33]. Studies using other plants of the same family, such as Momordica charantia (MC), have suggested that the blood-glucose-lowering effect of IS is through inhibition of glucose uptake, facilitation of glucose entrance into skeletal muscle cells, and increased insulin sensitivity. We also observed a lipid-lowering effect in HFF-fed mice, and this was mainly attributed to saponins present in the aqueous extract [34, 35]. Other authors also reported that fractions of MC inhibited disaccharidase, which had a marked influence on carbohydrate absorption and inhibited elevation of blood glucose. These results suggest that the main constituent of MC that underlies its hypoglycaemic effect could also inhibit pancreatic lipase and, consequently, the intestinal absorption of lipids thus contributes to the hypolipidemic and antiobesity properties of MC [34]. Because IS belongs to the same family as MC, it is expected to have similar constituents that could contribute to its activity. Hernández-Galicia et al. [21] reported that dichloromethane-extracted IS contained monoglycerides with antioxidant effects, which was observed only with the dose of 200 mg/kg, in our study.In conclusion, a HFF diet increased weight gain, cholesterol, triglycerides, blood glucose levels, and hepatic lipid peroxidation in mice. Aqueous extracts of IS (400 mg/kg) exhibited antiobesity effects and reduced glycaemia and hypotriglyceridemic effects in HFF-diet-induced obese and hyperglycaemic mice. The IS extract alone, at 400 mg/kg, decreased body weight and increased the atherogenic index. However, further investigations are required to assess the possible toxicity ofIbervillea sonorae.



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*Source: 968984-2011-11-17.xml*