# Nitrogen Immobilization in Plant Growth Substrates: Clean Chip Residual, Pine Bark, and Peatmoss

Authors: Cheryl R. Boyer; H. Allen Torbert; Charles H. Gilliam; Glenn B. Fain; Thomas V. Gallagher; Jeff L. Sibley
Journal: International Journal of Agronomy
 (2012)

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## Abstract

Rising costs of potting substrates have caused horticultural growers to search for alternative, lower-cost materials. Objectives of this study were to determine the extent of nitrogen immobilization and microbial respiration in a high wood-fiber content substrate, clean chip residual. Microbial activity and nitrogen availability of two screen sizes (0.95 cm and 0.48 cm) of clean chip residual were compared to control treatments of pine bark and peatmoss in a 60-day incubation experiment. Four rates (0, 1, 2, or 3 mg) of supplemental nitrogen were assessed. Peatmoss displayed little microbial respiration over the course of the study, regardless of nitrogen rate; followed by pine bark, 0.95 cm clean chip residual, and 0.48 cm clean chip residual. Respiration increased with increasing nitrogen. Total inorganic nitrogen (plant available nitrogen) was greatest with peatmoss; inorganic nitrogen in other treatments were similar at the 0, 1, and 2 mg supplemental nitrogen rates, while an increase occurred with the highest rate (3 mg). Clean chip residual and pine bark were similar in available nitrogen compared to peatmoss. This study suggests that nitrogen immobilization in substrates composed of clean chip residual is similar to pine bark and can be treated with similar fertilizer amendments during nursery production.


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## Body

## 1. Introduction

Pine bark (PB) and peatmoss (PM) have traditionally been used as nursery and greenhouse substrates in the US. These materials are becoming more costly to use in horticultural industries due to increasing fuel costs, reduced availability of PB [1], and environmental concerns over the use of PM for growing crops [2, 3]. Finding alternative substrates as a way to reduce costs has become an important issue for growers.One promising alternative substrate is CCR, a forest by-product of the ‘clean chip’ industry. The ‘clean chip’ industry processes small caliper pine trees into uniform, bark-free material for making paper products. This procedure is conducted on site at pine plantations with in-field harvesting equipment. This equipment delimbs, debarks, and chips the material into the back of a chip van/truck for shipment to a pulp mill. The remaining material, composed of approximately 40% wood, 35% bark, 10% needles, and 15% indistinguishable fine material, is either spread back across the harvested area or processed once more through a grinder with 10.2 to 15.2 cm screens and sold to the pulp mills for boiler fuel. Currently, this leftover material composes around 25% of the site biomass and represents an income loss for forest landowners.Clean chip residual has been evaluated (in a fresh state) for the production of several types of horticultural crops [4–7]. The residual material is obtained from loggers and further processed through a swinging hammer mill in order to produce material with reasonable particle size for horticultural use. Since this material is processed before use, it can be hammer milled to pass several different screen sizes, producing substrates that are suitable for a variety of crop types and container sizes. Boyer et al. [4] evaluated perennials, buddleia (Buddleja davidii ‘Pink Delight’), and verbena (Verbena canadensis ‘Homestead Purple’) in CCR and reported similar results among all treatments. A further study indicated that the use of supplemental N (in addition to standard control release fertilizer) was not necessary for growth of buddleia as compared to PB [5]. Later, Boyer et al. [6] demonstrated that annual plants, ageratum (Ageratum houstonianum ‘Blue Hawaii’), and salvia (Salvia  superba ‘Vista Purple’) grown in CCR or in combinations of CCR and PM produced similarly sized plants when compared to a traditional PB substrate treated with the same fertilizer regime. Woody plants such as loropetalum (Loropetalum chinensis var. rubrum) were also shown to have adequate growth in several screen sizes of CCR (compared to PB) over the course of one year [7].While crop growth in CCR has been equal to that displayed by plants grown in traditional substrates, questions remain regarding the high wood content of forest residuals (especially among growers). Since PB has a high lignin content, it is slow to decompose, and producing crops over a short-term growing season (and some long-term seasons) has not caused problems due to shrinkage of substrate during decomposition [8].Gruda et al. [9] reported significant N-immobilization resulting in less tomato (Lycopersicon lycopersicum) plant growth in substrates composed of a 100% wood fiber product. N-immobilization was calculated on the basis of N-balance including N-uptake by plants and residual mineral N in the substrates. Higher N-immobilization was found by increasing N-application rates. They determined that it is necessary to supply wood fiber substrates with nutrient solutions or fertilizer from the beginning of plant culture. Also, substrates without plants in this study (wood fiber and white PM) exposed to the same environmental conditions showed the same tendencies in N-immobilization as substrates with plants.Concern has arisen over whether the high wood content of CCR will immobilize N to an extent that plants experience a reduction in growth early in the crop cycle. This is especially important in greenhouse crops where the first few days and weeks are critical to long-term crop growth. The objective of this study was to determine the extent of N-immobilization in CCR, PB, and PM in order to make recommendations regarding how to overcome such a production problem.


## 2. Materials and Methods

Clean chip residual used in this study was obtained from a 10- to 12-year-old loblolly pine (Pinus taeda) plantation near Atmore, AL, which was thinned and processed for clean chips using a total tree harvester (Peterson DDC-5000-G Portable Chip Plant, Peterson Pacific Corp., Eugene, OR) and a horizontal grinder with 10.2 cm screens (Peterson 4700B Heavy Duty Horizontal Grinder, Peterson Pacific Corp., Eugene, OR). The material was further processed through a swinging hammer mill (no. 30; C.S. Bell, Tifton, OH) with either a 0.95 cm or 0.48 cm screen to produce two CCR products for testing. These two CCR particle sizes were compared with PB and PM (Table 1). Pine bark used in this study was obtained from Pineywoods Mulch Company (Alexander City, AL). Peatmoss was obtained from Premier Horticulture, Inc. (Quakertown, PA) and was tested to confirm that no supplemental N had been added prior to use in this study.Table 1
Physical properties of clean chip residual, pine bark, and peatmoss substrates.z
SubstratesyAir spacexContainer capacityw (% Vol)Total porosityvBulk density (g·cm−3)u0.48 cm CCR28 bt57 b85 b0.22 a0.95 cm CCR48 a42 d90 b0.19 bPB31 b48 c79 c0.18 bPM11 c87 a98 a0.11 czAnalysis performed using the North Carolina State University porometer.yCCR: clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.xAir space is the volume of water drained from the sample/volume of the sample.wContainer capacity is (wet weight−oven dry weight)/volume of the sample.vTotal porosity is container capacity  +  air space.uBulk density after forced-air drying at 105°C (221.0°F) for 48 h.tMeans within column followed by the same letter are not significantly different based on Waller-Duncan k ratio t-tests at α  =  0.05 (n  =  3).Substrate air space (AS), container capacity (CC), and total porosity (TP) were determined following procedures described by Bilderback et al. [10]. Substrate bulk density (BD) (g·cm−3) was determined from 347.5 g·cm−3 samples dried in a 105°C forced-air oven for 48 h. Substrates were analyzed for particle size distribution (PSD) by passing a 100 g air dried sample through 12.5, 9.5, 6.35, 3.35, 2.36, 2.0, 1.4, 1.0, 0.5, 0.25, and 0.11 mm sieves with particles passing the 0.11 mm sieve collected in a pan. Sieves were shaken for 3 min with a Ro-Tap (Ro-Tap RX-29, W.S. Tyler, Mentor, OH) sieve shaker (278 oscillations/min, 159 taps/min). Substrate samples (four reps per treatment) were further evaluated for soilless media nutrient analysis (Brookside Laboratories, Inc., New Knoxville, OH). The material was ground and screened before saturated media extracts (water based) were prepared from the samples. Substrate pH, EC, NO3, NH4, and all other nutrients were measured using this water extract. Plant available NO3 and NH4 were determined using flow injection analysis (FIAlab-2500, FIAlab Instruments, Bellevue, WA), and Ca, Mg, P, K, Na, SO4, B, Mn, Zn, Fe, and Cu were determined by microwave digestion with inductively coupled plasma-emission spectrometry (ICP) (Thermo Jarrell Ash 6500 ICAP series, Offenbach, Germany).An incubation study was conducted at the USDA-ARS National Soil Dynamics Laboratory in Auburn, AL to determine N mineralization/immobilization and microbial activity of each of the four substrate materials. A completely randomized design (CRD) with 272 experimental units was conducted. Treatment structure was factorial with four substrates by four N rates by four sample dates, each with four replications. There were also 16 units (four for each collection date) without substrate, serving as ambient CO2 traps. The incubation procedure consisted of weighing 20 g (dry weight basis) of substrate into plastic containers. Deionized water was added to adjust samples to consistent moisture content. Moisture content was determined by saturating 20 g of each substrate with deionized water and recording wet weight (after draining to simulate CC) and dry weight (after drying in an oven at 105°C for 48 h). The change in weight (wet minus dry) divided by the wet weight multiplied by 100 equals the percent (%) of moisture content (average of three subsamples). Each substrate had different percent of moisture contents, and subsequently an appropriate amount of deionized water was added to each sample in order to bring the moisture of the substrate up to CC. Container capacity is the amount of water in a just-drained container substrate. Four rates of supplemental N (0, 1, 2, and 3 mg of N added by the addition of 0, 0.5, 1.0, or 1.5 mL of 2000 ppm stock solution of NH4NO3) were added to each of the four substrates in the study. The containers were placed in sealed glass jars with 10 mL of water for humidity control and a vial containing 10 mL of 1 M NaOH as a CO2 trap. The jars were incubated in the dark at 25°C and removed after 7, 15, 30, and 60 days. Carbon mineralization, which is a direct measurement of total microbial respiration, was measured in this study. Carbon dioxide in the NaOH traps was determined by titrating the excess base with 1 M HCl in the presence of BaCl2. All traps were measured at each sampling date, and fresh NaOH traps were placed in the jars remaining to be sampled at later dates. At each sampling date, a set of samples were measured for inorganic N concentration. Samples were extracted with a 2 N KCl solution and measured for NH4-N and NO3-N using a Model 680 Microplate Reader (Bio-Rad Laboratories Inc., Hercules, CA). Inorganic N was calculated as the sum of NH4 and NO3. Potential N mineralization was the difference between final and initial inorganic N contents.Data were analyzed using Waller-Duncank ratio t-tests (P≤0.05) and a statistical software package (SAS Institute, Cary, NC). Data were analyzed separately for each sampling date.


## 3. Results and Discussion

### 3.1. Substrate Physical and Chemical Properties

Physical properties of the four substrates tested varied (Table1). Each substrate had significantly different AS with 0.95 cm CCR having the greatest (48%) and PM having the least (11%). Container capacity was also different for each substrate; however, PM had the greatest CC (87%), as expected, and PB had the least CC (48%). Both CCR treatments were similar in TP but were between of 98% for PM and 79% for PB. Bulk density was greatest for 0.48 cm CCR (0.22 g·cm−3) and least for PM (0.11 g·cm−3).Particle size analysis revealed that 0.48 cm CCR had the least amount of coarse particles (0.8%), while PM had the greatest amount of coarse particles (38%) (Table2). Pine bark and 0.95 cm CCR were similar (30% and 27%) for coarse particles. Both CCR substrates had the highest amount of medium-sized particles (48% for 0.48 cm and 50% for 0.95 cm). Pine bark had 38% medium-sized particles and PM had 31%. The greatest percentage of fine particles was measured in 0.48 cm CCR (51%) followed by PM (31%), PB (32%), and 0.95 cm CCR (24%).Table 2
Particle size analysis of clean chip residual, pine bark, and peatmoss substrates.
U.S. standard sieve no.Sieve opening (mm)Substratez0.48 cm CCR0.95 cm CCRPBPM1/212.500.0 ay0.0 a0.0 a2.2 a3/89.500.0 b0.0 b0.1 b8.2 a1/46.350.0 d2.7 c6.0 b11.0 a63.350.8 c23.9 a24.0 a17.0 b82.368.8 c20.0 a12.6 b9.1 c102.007.9 a8.1 a5.0 b3.5 c141.4019.0 a13.2 b11.3 c9.0 d181.0012.4 a8.2 b9.1 b9.0 b350.5013.0 b7.7 c13.8 ab15.1 a600.2512.1 a7.8 b8.4 b9.4 b1400.1115.1 a7.0 b5.1 b5.0 b2700.055.8 a0.9 c2.5 b1.1 cpan0.005.1 a0.5 c2.1 b0.4 cTexturexCoarse0.8 c26.6 b30.0 b38.3 aMedium48.0 a49.5 a38.0 b31.0 cFine51.2 a23.9 c32.0 b30.7 bzCCR: clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.yPercent weight of sample collected on each screen, means within row followed by the same letter are not significantly different based on Waller-Duncan k ratio t-tests at α  =  0.05 (n  =  3).xCoarse: >3.35 mm, Medium:  >1.00–<3.35 mm and Fine:  <1.0 mm.Substrate pH was significantly different for each substrate (Table3). Clean chip residual screened at 0.95 cm had the highest pH (5.5) while 0.48 cm had a pH of 5.0. Peatmoss had the next highest pH (4.8) while PB had the lowest pH (4.1). Peatmoss and PB generally require lime addition to raise the pH for adequate plant culture. This may not be needed for substrates composed of CCR as its pH is already in an acceptable range for plant growth. Electrical conductivity was in the typical range for plant production, though each substrate was different (from 0.15 to 0.29 mS·cm−1).Table 3
Chemical properties of clean chip residual, pine bark, and peatmoss substrates.
SubstratezpHEC (mS·cm−1)ySubstrate micronutrient contentxB (ppm)Fe (ppm)Mn (ppm)Cu (ppm)Zn (ppm)0.48 cm CCR5.0 bw0.21 b0.23 a3.7 a2.3 a0.01 b0.28 a0.95 cm CCR5.5 a0.15 c0.17 b2.1 b0.6 b0.02 ab0.06 cPB4.1 d0.23 b0.16 b1.3 c1.0 b0.03 a0.16 bPM4.8 c0.29 a0.13 c0.2 d0.2 c0.01 b0.05 cSubstrate macronutrient contentNO3-N (ppm)NH4-N (ppm)P (ppm)K (ppm)Ca (ppm)Mg (ppm)SO4 (ppm)0.48 cm CCR0.1 b0.2 b4.1 a84.6 a9.1 c7.0 c15.8 c0.95 cm CCR0.2 b0.4 a1.4 b48.2 b5.3 d2.6 d7.7 dPB0.1 b0.1 b4.0 a52.5 b16.2 b11.5 b50.9 aPM39.0 a0.1 a1.6 b6.9 c27.7 a28.3 a29.5 bzCCR:  clean chip residual, PB:  pine bark, PM:  sphagnum peatmoss.y1 mS·cm−1  =  1 mmho/cm.xSubstrate analysis performed on nonamended samples; N:  nitrogen, P:  phosphorous, K:  potassium, Ca:  calcium, Mg:  magnesium, S:  sulfur, B:  boron, Fe:  iron, Mn:  manganese, Cu:  copper, Zn:  zinc, and 1 ppm  =  1 mg·kg−1.wMeans within column followed by the same letter are not significantly different based on Waller-Duncan k ratio t-tests (α  =  0.05, n  =  3).Substrate chemical analysis revealed that PM had a significantly higher amount of NO3-N (39.0 ppm) than all other treatments (0.1-0.2 ppm) (Table 3). Values for NH4-N were all low (from 0.1 to 0.4 ppm). Potassium was high in all substrates except PM (48.2–84.6 ppm versus 6.9 ppm). Calcium was greatest in PM (27.7 ppm) and least in the CCR treatments (from 5.3 to 9.1 ppm). Magnesium was also greatest in PM (28.3 ppm) and least in CCR (2.6 to 7.0 ppm). Sulfur was high in PB (50.9 ppm) and low in 0.95 cm CCR (7.7%). Iron, Mn, and Zn were higher in 0.48 cm CCR than all other treatments; however, these differences are not meaningful in a production context.


### 3.2. Microbial Respiration

Microbial respiration (MR) was evaluated at each sampling date (Table4). Peatmoss consistently had the least microbial respiration regardless of date or supplemental N rate. The greatest microbial respiration occurred with the CCR treatments. As particle size decreased (0.48 cm), microbial respiration increased. Also, as N rate increased, microbial respiration increased in CCR and PB.Table 4
Microbial respiration in clean chip residual, pine bark and peatmoss substrates.
SubstratezCarbon mineralization (mg/kg)0 mg N1 mg Ny2 mg N3 mg NMSD N-ratex0–7 days0.48 cm CCR23703236358438821130.95 cm CCR1757259329873414154PB1918231123672478111PM14394614258335MSD Substrate841212841708–15 days0.48 cm CCR26152827288833581830.95 cm CCR2200262031053706209PB1625198619442021329PM356384353371117MSD Substrate28314017214916–30 days0.48 cm CCR34793615363134361340.95 cm CCR3229355637283714225PB2254248525852586129PM751591578641166MSD Substrate19513912312631–60 days0.48 cm CCR41333812372138374590.95 cm CCR4082421140413767324PB2950317333563417190PM1723168215811582258MSD Substrate355166238248Total: 0–60 days0.48 cm CCR123601341413778141086090.95 cm CCR11016131101437714624799PB8954100971031310484422PM2989292227622781440MSD Substrate668405662409zCCR:  clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.y2000 ppm stock solution of NH4NO3 (0, 0.5, 1.0, 1.5 mL).xMSD based on Waller-Duncan k ratio t-tests (α  =  0.05).Microbial respiration at 7 days after treatment (DAT) showed that at each N rate, 0.48 cm CCR had the greatest microbial respiration followed by 0.95 cm CCR, PB, and PM (Table4). For 0.48 cm and 0.95 cm CCR, microbial respiration was highest with 2 mg N and decreased significantly as N rate decreased. Pine bark had significantly higher microbial respiration at 2 mg N than at 0 and 1 mg N (each was different from each other).At 15 DAT, 0.48 cm CCR (0 and 1 mg N rates) had the greatest microbial respiration followed by 0.95 cm CCR, PB, and PM (Table4). At 2 and 3 mg N, the CCR treatments switched with 0.95 cm CCR having more microbial respiration than 0.48 cm. For 0.48 cm and 0.95 cm CCR, MR was highest with 2 mg N and decreased significantly as N rate decreased. Pine bark microbial respiration was similar at the three highest N rates, and although 0 and 1 mg N had less microbial respiration they were similar to each other. There were no differences in microbial respiration for PM at any N rate.The greatest microbial respiration at 30 DAT for 0 mg N was with 0.48 cm CCR followed by 0.95 cm CCR, PB, and PM (Table4). At 1 and 2 mg N both CCR treatments were similar, but PB followed by PM had less microbial respiration. At the highest N rate, 0.95 cm CCR had the greatest microbial respiration followed by 0.48 cm CCR, PB, and PM. For 0.48 cm CCR the greatest microbial respiration was with 1 and 2 mg N, while 0 and 3 mg N had less microbial respiration. For 0.95 cm CCR and PB only 0 mg N was significantly less than other N rates. There were no differences in microbial respiration for PM at any N rate.Microbial respiration at 60 DAT showed that at 0 and 3 mg N rate, both CCR treatments had the highest microbial respiration, followed by PB and PM (Table4). At 1 and 2 mg N rates, 0.95 cm CCR had the greatest microbial respiration followed by 0.48 cm CCR, PB, and PM. For 0.48 cm CCR and PM there were no differences across N rates. For 0.95 cm CCR, only the highest rate of N had less microbial respiration than the other rates, though the values were similar to 0 and 2 mg N.Clean chip residual consistently had the greatest amount of microbial respiration among the substrates over the course of the incubation period (0–60 DAT) (Table4). At 0 mg N rate, 0.48 cm CCR had greater microbial respiration than 0.95 cm, but at 1 and 2 mg N it was similar. At 3 mg N, 0.95 cm CCR had greater microbial respiration than 0.48 cm CCR. Pine bark and PM were different from each other and less than CCR treatments for microbial respiration. Across the N rates for 0.48 cm CCR, microbial respiration increased with increasing N rate. For 0.95 cm CCR, microbial respiration increased with increasing N rate, though 2 and 3 mg N were similar. Pine bark was similar at 1, 2, and 3 mg N rates, only 0 mg N had less microbial respiration. There were no differences in microbial respiration across N rates for PM.


### 3.3. Total Inorganic N (Plant Available N)

At 7 DAT, PM had more N than all other treatments; all other treatments were similar (Table5). At 2 and 3 mg N, PM had the most N followed by PB and the CCR treatments, which were similar to each other. Across N rates for 0.48 cm CCR and PB, 3 mg N had more N than other rates, which were similar. For 0.95 cm CCR, N increased with increasing N rate, though 0 and 2 mg N were similar. Peatmoss had greater available N as N rate increased.Table 5
Total inorganic nitrogen (NH4 and NO3) mineralization in clean chip residual, pine bark and peatmoss substrates.
SubstratezNitrogen mineralization (mg/kg)0 mg N1 mg Ny2 mg N3 mg NMSD N-ratex0–7 days0.48 cm CCR312837128160.95 cm CCR4477262515174PB44564401458459PM1753254933595264790MSD Substrate1301742508500–15 days0.48 cm CCR58614250550.95 cm CCR53526416879PB5882931572442PM1619293736105869588MSD Substrate1692811696300–30 days0.48 cm CCR1011489980670.95 cm CCR114116102310119PB75108152106182PM2530359140436149768MSD Substrate3424293564020–60 days0.48 cm CCR638791117440.95 cm CCR9114212114234PB33395876194PM1806263235335404783MSD Substrate44150205734zCCR:  clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.y2000 ppm stock solution of NH4NO3 (0, 0.5, 1.0, 1.5 mL).xMSD based on Waller-Duncan k ratio t-tests (α  =  0.05).Total inorganic N at 15 DAT showed that at 0 and 1 mg N PM had the greatest amount of available N followed by PB and CCR treatments, which were all similar to each other (Table5). At 2 and 3 mg N, PM had the highest N followed by PB and the two CCR treatments, which were similar to each other. There was no significant difference in available N for 0.48 cm CCR across N rates. For 0.95 cm CCR and PB, the greatest amount of available N was with 3 mg N, all other N rates had less N and were similar to each other. Peatmoss had increasingly available N as N rate increased.At 30 DAT, results for total available N were similar to results at 15 DAT, with one exception: among substrates treated with 2 mg supplemental N, PM had the greatest amount of N and all other treatments were similar (Table5). Also for PM, 1 and 2 mg N were similar, though the trend continued toward having more available N as N rate increased.Peatmoss had the most available N at all N rates among substrates at 60 DAT (Table5). At 0 mg N, 0.95 cm CCR had greater N than PB. For all other N rates, CCR and PB had similar available N, though less than PM. Across N rates, there were no differences for 0.48 cm CCR. For the remainder of the substrates, available N increased with increasing N rates.


## 3.1. Substrate Physical and Chemical Properties

Physical properties of the four substrates tested varied (Table1). Each substrate had significantly different AS with 0.95 cm CCR having the greatest (48%) and PM having the least (11%). Container capacity was also different for each substrate; however, PM had the greatest CC (87%), as expected, and PB had the least CC (48%). Both CCR treatments were similar in TP but were between of 98% for PM and 79% for PB. Bulk density was greatest for 0.48 cm CCR (0.22 g·cm−3) and least for PM (0.11 g·cm−3).Particle size analysis revealed that 0.48 cm CCR had the least amount of coarse particles (0.8%), while PM had the greatest amount of coarse particles (38%) (Table2). Pine bark and 0.95 cm CCR were similar (30% and 27%) for coarse particles. Both CCR substrates had the highest amount of medium-sized particles (48% for 0.48 cm and 50% for 0.95 cm). Pine bark had 38% medium-sized particles and PM had 31%. The greatest percentage of fine particles was measured in 0.48 cm CCR (51%) followed by PM (31%), PB (32%), and 0.95 cm CCR (24%).Table 2
Particle size analysis of clean chip residual, pine bark, and peatmoss substrates.
U.S. standard sieve no.Sieve opening (mm)Substratez0.48 cm CCR0.95 cm CCRPBPM1/212.500.0 ay0.0 a0.0 a2.2 a3/89.500.0 b0.0 b0.1 b8.2 a1/46.350.0 d2.7 c6.0 b11.0 a63.350.8 c23.9 a24.0 a17.0 b82.368.8 c20.0 a12.6 b9.1 c102.007.9 a8.1 a5.0 b3.5 c141.4019.0 a13.2 b11.3 c9.0 d181.0012.4 a8.2 b9.1 b9.0 b350.5013.0 b7.7 c13.8 ab15.1 a600.2512.1 a7.8 b8.4 b9.4 b1400.1115.1 a7.0 b5.1 b5.0 b2700.055.8 a0.9 c2.5 b1.1 cpan0.005.1 a0.5 c2.1 b0.4 cTexturexCoarse0.8 c26.6 b30.0 b38.3 aMedium48.0 a49.5 a38.0 b31.0 cFine51.2 a23.9 c32.0 b30.7 bzCCR: clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.yPercent weight of sample collected on each screen, means within row followed by the same letter are not significantly different based on Waller-Duncan k ratio t-tests at α  =  0.05 (n  =  3).xCoarse: >3.35 mm, Medium:  >1.00–<3.35 mm and Fine:  <1.0 mm.Substrate pH was significantly different for each substrate (Table3). Clean chip residual screened at 0.95 cm had the highest pH (5.5) while 0.48 cm had a pH of 5.0. Peatmoss had the next highest pH (4.8) while PB had the lowest pH (4.1). Peatmoss and PB generally require lime addition to raise the pH for adequate plant culture. This may not be needed for substrates composed of CCR as its pH is already in an acceptable range for plant growth. Electrical conductivity was in the typical range for plant production, though each substrate was different (from 0.15 to 0.29 mS·cm−1).Table 3
Chemical properties of clean chip residual, pine bark, and peatmoss substrates.
SubstratezpHEC (mS·cm−1)ySubstrate micronutrient contentxB (ppm)Fe (ppm)Mn (ppm)Cu (ppm)Zn (ppm)0.48 cm CCR5.0 bw0.21 b0.23 a3.7 a2.3 a0.01 b0.28 a0.95 cm CCR5.5 a0.15 c0.17 b2.1 b0.6 b0.02 ab0.06 cPB4.1 d0.23 b0.16 b1.3 c1.0 b0.03 a0.16 bPM4.8 c0.29 a0.13 c0.2 d0.2 c0.01 b0.05 cSubstrate macronutrient contentNO3-N (ppm)NH4-N (ppm)P (ppm)K (ppm)Ca (ppm)Mg (ppm)SO4 (ppm)0.48 cm CCR0.1 b0.2 b4.1 a84.6 a9.1 c7.0 c15.8 c0.95 cm CCR0.2 b0.4 a1.4 b48.2 b5.3 d2.6 d7.7 dPB0.1 b0.1 b4.0 a52.5 b16.2 b11.5 b50.9 aPM39.0 a0.1 a1.6 b6.9 c27.7 a28.3 a29.5 bzCCR:  clean chip residual, PB:  pine bark, PM:  sphagnum peatmoss.y1 mS·cm−1  =  1 mmho/cm.xSubstrate analysis performed on nonamended samples; N:  nitrogen, P:  phosphorous, K:  potassium, Ca:  calcium, Mg:  magnesium, S:  sulfur, B:  boron, Fe:  iron, Mn:  manganese, Cu:  copper, Zn:  zinc, and 1 ppm  =  1 mg·kg−1.wMeans within column followed by the same letter are not significantly different based on Waller-Duncan k ratio t-tests (α  =  0.05, n  =  3).Substrate chemical analysis revealed that PM had a significantly higher amount of NO3-N (39.0 ppm) than all other treatments (0.1-0.2 ppm) (Table 3). Values for NH4-N were all low (from 0.1 to 0.4 ppm). Potassium was high in all substrates except PM (48.2–84.6 ppm versus 6.9 ppm). Calcium was greatest in PM (27.7 ppm) and least in the CCR treatments (from 5.3 to 9.1 ppm). Magnesium was also greatest in PM (28.3 ppm) and least in CCR (2.6 to 7.0 ppm). Sulfur was high in PB (50.9 ppm) and low in 0.95 cm CCR (7.7%). Iron, Mn, and Zn were higher in 0.48 cm CCR than all other treatments; however, these differences are not meaningful in a production context.


## 3.2. Microbial Respiration

Microbial respiration (MR) was evaluated at each sampling date (Table4). Peatmoss consistently had the least microbial respiration regardless of date or supplemental N rate. The greatest microbial respiration occurred with the CCR treatments. As particle size decreased (0.48 cm), microbial respiration increased. Also, as N rate increased, microbial respiration increased in CCR and PB.Table 4
Microbial respiration in clean chip residual, pine bark and peatmoss substrates.
SubstratezCarbon mineralization (mg/kg)0 mg N1 mg Ny2 mg N3 mg NMSD N-ratex0–7 days0.48 cm CCR23703236358438821130.95 cm CCR1757259329873414154PB1918231123672478111PM14394614258335MSD Substrate841212841708–15 days0.48 cm CCR26152827288833581830.95 cm CCR2200262031053706209PB1625198619442021329PM356384353371117MSD Substrate28314017214916–30 days0.48 cm CCR34793615363134361340.95 cm CCR3229355637283714225PB2254248525852586129PM751591578641166MSD Substrate19513912312631–60 days0.48 cm CCR41333812372138374590.95 cm CCR4082421140413767324PB2950317333563417190PM1723168215811582258MSD Substrate355166238248Total: 0–60 days0.48 cm CCR123601341413778141086090.95 cm CCR11016131101437714624799PB8954100971031310484422PM2989292227622781440MSD Substrate668405662409zCCR:  clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.y2000 ppm stock solution of NH4NO3 (0, 0.5, 1.0, 1.5 mL).xMSD based on Waller-Duncan k ratio t-tests (α  =  0.05).Microbial respiration at 7 days after treatment (DAT) showed that at each N rate, 0.48 cm CCR had the greatest microbial respiration followed by 0.95 cm CCR, PB, and PM (Table4). For 0.48 cm and 0.95 cm CCR, microbial respiration was highest with 2 mg N and decreased significantly as N rate decreased. Pine bark had significantly higher microbial respiration at 2 mg N than at 0 and 1 mg N (each was different from each other).At 15 DAT, 0.48 cm CCR (0 and 1 mg N rates) had the greatest microbial respiration followed by 0.95 cm CCR, PB, and PM (Table4). At 2 and 3 mg N, the CCR treatments switched with 0.95 cm CCR having more microbial respiration than 0.48 cm. For 0.48 cm and 0.95 cm CCR, MR was highest with 2 mg N and decreased significantly as N rate decreased. Pine bark microbial respiration was similar at the three highest N rates, and although 0 and 1 mg N had less microbial respiration they were similar to each other. There were no differences in microbial respiration for PM at any N rate.The greatest microbial respiration at 30 DAT for 0 mg N was with 0.48 cm CCR followed by 0.95 cm CCR, PB, and PM (Table4). At 1 and 2 mg N both CCR treatments were similar, but PB followed by PM had less microbial respiration. At the highest N rate, 0.95 cm CCR had the greatest microbial respiration followed by 0.48 cm CCR, PB, and PM. For 0.48 cm CCR the greatest microbial respiration was with 1 and 2 mg N, while 0 and 3 mg N had less microbial respiration. For 0.95 cm CCR and PB only 0 mg N was significantly less than other N rates. There were no differences in microbial respiration for PM at any N rate.Microbial respiration at 60 DAT showed that at 0 and 3 mg N rate, both CCR treatments had the highest microbial respiration, followed by PB and PM (Table4). At 1 and 2 mg N rates, 0.95 cm CCR had the greatest microbial respiration followed by 0.48 cm CCR, PB, and PM. For 0.48 cm CCR and PM there were no differences across N rates. For 0.95 cm CCR, only the highest rate of N had less microbial respiration than the other rates, though the values were similar to 0 and 2 mg N.Clean chip residual consistently had the greatest amount of microbial respiration among the substrates over the course of the incubation period (0–60 DAT) (Table4). At 0 mg N rate, 0.48 cm CCR had greater microbial respiration than 0.95 cm, but at 1 and 2 mg N it was similar. At 3 mg N, 0.95 cm CCR had greater microbial respiration than 0.48 cm CCR. Pine bark and PM were different from each other and less than CCR treatments for microbial respiration. Across the N rates for 0.48 cm CCR, microbial respiration increased with increasing N rate. For 0.95 cm CCR, microbial respiration increased with increasing N rate, though 2 and 3 mg N were similar. Pine bark was similar at 1, 2, and 3 mg N rates, only 0 mg N had less microbial respiration. There were no differences in microbial respiration across N rates for PM.


## 3.3. Total Inorganic N (Plant Available N)

At 7 DAT, PM had more N than all other treatments; all other treatments were similar (Table5). At 2 and 3 mg N, PM had the most N followed by PB and the CCR treatments, which were similar to each other. Across N rates for 0.48 cm CCR and PB, 3 mg N had more N than other rates, which were similar. For 0.95 cm CCR, N increased with increasing N rate, though 0 and 2 mg N were similar. Peatmoss had greater available N as N rate increased.Table 5
Total inorganic nitrogen (NH4 and NO3) mineralization in clean chip residual, pine bark and peatmoss substrates.
SubstratezNitrogen mineralization (mg/kg)0 mg N1 mg Ny2 mg N3 mg NMSD N-ratex0–7 days0.48 cm CCR312837128160.95 cm CCR4477262515174PB44564401458459PM1753254933595264790MSD Substrate1301742508500–15 days0.48 cm CCR58614250550.95 cm CCR53526416879PB5882931572442PM1619293736105869588MSD Substrate1692811696300–30 days0.48 cm CCR1011489980670.95 cm CCR114116102310119PB75108152106182PM2530359140436149768MSD Substrate3424293564020–60 days0.48 cm CCR638791117440.95 cm CCR9114212114234PB33395876194PM1806263235335404783MSD Substrate44150205734zCCR:  clean chip residual, PB:  pine bark, and PM:  sphagnum peatmoss.y2000 ppm stock solution of NH4NO3 (0, 0.5, 1.0, 1.5 mL).xMSD based on Waller-Duncan k ratio t-tests (α  =  0.05).Total inorganic N at 15 DAT showed that at 0 and 1 mg N PM had the greatest amount of available N followed by PB and CCR treatments, which were all similar to each other (Table5). At 2 and 3 mg N, PM had the highest N followed by PB and the two CCR treatments, which were similar to each other. There was no significant difference in available N for 0.48 cm CCR across N rates. For 0.95 cm CCR and PB, the greatest amount of available N was with 3 mg N, all other N rates had less N and were similar to each other. Peatmoss had increasingly available N as N rate increased.At 30 DAT, results for total available N were similar to results at 15 DAT, with one exception: among substrates treated with 2 mg supplemental N, PM had the greatest amount of N and all other treatments were similar (Table5). Also for PM, 1 and 2 mg N were similar, though the trend continued toward having more available N as N rate increased.Peatmoss had the most available N at all N rates among substrates at 60 DAT (Table5). At 0 mg N, 0.95 cm CCR had greater N than PB. For all other N rates, CCR and PB had similar available N, though less than PM. Across N rates, there were no differences for 0.48 cm CCR. For the remainder of the substrates, available N increased with increasing N rates.


## 4. Discussion

Incubation studies have previously been used to evaluate N-immobilization for horticultural purposes. A study by Hartz and Giannini [11] reported short-term net N-immobilization (2-week aerobic incubation) in samples of composted municipal yard and landscape wastes from three locations, with an overall trend toward decreased immobilization with increased compost age. At least 9–12 weeks of composting were required to minimize the undesirable characteristics of immature compost. Compost materials generally provide enough N to negate the use of supplemental fertilizer; however, materials such as PB, PM, and CCR do not contain adequate N to support plant growth and require fertilization before use in plant production.A subsequent study by Hartz et al. [12] determined the N and C mineralization rates of 19 manure and compost samples for use as soil amendments in vegetable production in 1996 and 12 additional samples in 1997. Net N mineralization was measured at 4- or 8-week intervals while C mineralization was measured at 4-week intervals. An average of 16%, 7%, and 1% of organic N was mineralized after 12 weeks in 1996, and an average of 15%, 6%, and 2% was mineralized after 24 weeks of incubation for 1997, in manure, manure compost, and plant residue compost, respectively. Mineralization of manure C averaged 35% of initial C content after 24 weeks, while compost averaged only 14%. Within 4 (compost) or 16 weeks (manure), the rate of mineralization of amendment C had declined to a level similar to soil organic C.Waste paper as a substitute for PM displays significant N-immobilization and high pH [13]. An incubation study was conducted to define the N status of the paper medium. Initial results indicated diminished plant growth in pure paper substrate. The amount of additional N needed was difficult to predict during cultivation. A composting process was deemed necessary to overcome N-immobilization and lower pH and to improve water-conducting properties of the substrate.When manure compost is used, N-immobilization will eventually stop and N-mineralization will begin. In the previous cases, N was provided to the plant instead of being removed, resulting in competition between plants and microbes for N. Our results indicate that N became available for 0.95 cm CCR during the course of our study, but the change was small relative to the loss of N from PM.While CCR exhibits high microbial respiration (particularly with smaller particle sizes), microbial activity and N-immobilization were generally similar to PB. Clean chip residual screened to 0.48 cm was more microbially active, most likely due to increased surface area resulting from smaller particle size. Since CCR has an inherently high percentage of PB (35%), it tends to perform similarly; the addition of 40% wood fiber does not seem to inhibit plant growth or require amendment changes in nursery crop production. This study indicated that almost no management differences should be expected for crops produced in CCR compared to PB.Peatmoss had virtually no microbial activity as measured by respiration in this study. However, unlike PB and CCR this was not due to N limitation as there was no indication that respiration was impacted at all by the addition of N. While it is clear that the remaining materials produced significant immobilization of the fertilizer N, there was little or no difference between PB and CCR. While PB did have increased N levels at the highest supplemental rate, this was miniscule compared to PM and does not imply a need to change management. In fact, N levels in PB decreased over time compared to CCR, which showed slow increases in N. For example, at 2 mg supplemental N PB had 440 mg·kg−1 available N at 7 DAT, which was reduced to 58 mg·kg−1 by day 60. On the other hand, CCR had 37 mg·kg−1 at 7 DAT but increased to 91 mg·kg−1 by 60 DAT. This indicates that while the immobilization of N was similar in PB and CCR, it may have increased in PB over time. This is reflected in respiration where there initially was much lower microbial respiration from PB, but by 31–60 DAT the respiration rate was similar to CCR and much more responsive to changes in supplemental N rate. This indicates that PB became more inclined toward N-immobilization as time progressed.In the current study, pH and EC of CCR were determined to be acceptable for plant culture. In fact, CCR may not require a limestone amendment to raise pH. Since 0.95 cm CCR and PB have similar particle size distributions, we recommend this screen size for 1-gal. containers on outdoor beds, while 0.48 cm CCR is more suitable for greenhouse production. Since many PM suppliers premix amendments into shipped products, it is essential to determine the correct fertilization amendments so that substrates composed of CCR can perform similarly to PM. The results of this study indicate that growers should not be concerned about short-term negative impacts from N-immobilization in CCR (compared to PB).



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*Source: 978528-2012-05-16.xml*