# Alkaloids inErythrina by UPLC-ESI-MS and In Vivo Hypotensive Potential of Extractive Preparations

Authors: Liara Merlugo; Marí C. Santos; Liane S. Sant’Anna; Everson W. F. Cordeiro; Luiz A. C. Batista; Silvia T. S. Miotto; Cássia V. Garcia; Cleci M. Moreira; Andreas S. L. Mendez
Journal: Evidence-Based Complementary and Alternative Medicine
 (2015)

---

## Abstract

Erythrina species are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves of E. falcata and E. crista-galli. The hypotensive potential of E. falcata and the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. For in vivo protocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v. The results of total phenolic contents for E. falcata and E. crista-galli were 1.3193–1.4989 mgGAE/mL for maceration and 0.8771–0.9506 mgGAE/mL for infusion. In total flavonoids, the content was 7.7829–8.1976 mg RE/g for maceration and 9.3471–10.4765 RE mg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11β-methoxyglucoerysodine, erysothiopine, 11β-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed for E. falcata, which may be related to the route of β-adrenergic receptors.


---

## Body

## 1. Introduction

Hypertension is a global health problem whose increased incidence can lead to the development of many chronic diseases, as well as collaborating in the progression of chronic kidney disease, and contribute to cardiovascular morbidity and mortality [1]. Although there is a wide range of antihypertensive drugs used in the control of hypertension, a successful treatment becomes even harder to achieve [2], so the knowledge of medicinal plants, their composition, and mechanism of action could have a vital role in the discovery of new products as therapeutic agents.The genusErythrina (Fabaceae) consists of 110 species, distributed in the tropical regions of America, Africa, Asia, and Oceania [3, 4]. Phytochemical studies of its different species showed a chemical constitution by alkaloids, flavonoids, pterocarpans, and cumarines [5–9]. Species asE. velutina, E. mulungu, E. crista-galli, andE. falcata demonstrated the predominance of alkaloids and flavonoids in their composition [10–14]. The alkaloids present inErythrina species have attracted much attention from researchers, since they represent the main source of the tetracyclic alkaloids ofErythrina type and have similar activities of the curare, causing muscle paralysis [15].In popular medicine, these species are used for the treatment of respiratory diseases, as well as anxiolytic, sedative, anti-inflammatory, and antihypertensive [11, 16]. Scientific studies showed different activities for these species such as antibacterial, antifungal, and anti-inflammatory [17–19]. Lehman [20] tested alcoholic extracts ofE. americana and proved its hypotensive effect in rats, rabbits, and dogs. Alkaloids obtained from this species also demonstrated hypotensive activity [21].The present study aimed to investigate the chemical composition of the speciesE. falcata andE. crista-galli by UPLC-ESI-MS and to evaluate the hemodynamic parameters in rats, purposing to elucidate the involved mechanism of action forE. falcata extracts.


## 2. Materials and Methods

### 2.1. Plant Material

Erythrina crista-galli was collected in May 2013 in Uruguaiana, RS (Brazil).Erythrina falcata was collected in Porto Alegre, RS (Brazil), in February 2014. The materials were identified and a voucher specimen of each (Erythrina crista-galli, ICN 183940;Erythrina falcata Benth., ICN 177665) was deposited at the ICN Herbarium (Instituto de Biociências, UFRGS, Avenida Bento Gonçalves, 9500, Prédio 43433, CEP 91501-970, Porto Alegre, RS, Brazil).


### 2.2. Extracts Preparation

Leaves were selected and dried at a temperature of 40°C for five days. After that, they were grounded and submitted to exhaustive extraction processes by maceration using 40% ethanol as solvent (1 : 10, plant : solvent) and by infusion using distilled water at a temperature of 100°C (1 : 15, plant : solvent).For chromatographic assays, extracts obtained by maceration process of both species were used. To reduce the impurities, extracts passed through a liquid-liquid extraction method by affinity solvents with increasing polarity (hexane, dichloromethane, ethyl acetate, and methanol) and for further analysis they were filtered on a membrane of 0.45 micrometers.For biological analyses macerations were used and infusions were obtained fromE. falcata, which were oven-dried and reduced to powder for subsequent redilution in 0.9% saline solution for administration.


### 2.3. Total Phenolic Content

The total phenolic content was determined by Folin-Ciocalteu colorimetric method with some modifications [22]. For the analyses, aliquots of 100 μL of the samples and standard solutions were transferred to test tubes, where 500 μL of Folin-Ciocalteu reagent and 6 mL of distilled water were added. Then, they were agitated and left at rest for 1 minute. After alkalizing the medium, 2 mL of Na2CO3 solution 15% was added, and the volume was completed to 10 mL with distilled water. After 30 minutes at room temperature and being protected from light, samples were analysed in a spectrophotometer Perkin Elmer UV-VIS Lambda 35 (Norwalk, CT, USA) at 750 nm. The total phenolic content was expressed in milligrams of gallic acid equivalent per mL of sample (mgGAE/mL).


### 2.4. Total Flavonoid Content

The total flavonoid content was determined by the method described by Chang et al. [23] with some modifications. In a 25 mL flask 500 μL of sample and 500 μL of AlCl3 0.5% were added and the volume was completed with water. It was incubated for 40 minutes at room temperature and protected from light and reading was done in UV-VIS spectrophotometer Lambda 35 Perkin Elmer (Norwalk, CT, USA) at a wavelength of 415 nm. The results were expressed in mg/g equivalents of rutin.


### 2.5. UPLC-MS

For the protocols a reverse-phase system was performed, using the following conditions: fast C18 analytical column Shim-pack XR-ODS (50 × 2 mm, 2.1μm); mobile phase consisted of acetonitrile : methanol 4 : 1 (v/v) and water with 0.1% formic acid (pH 3.0) with a flow rate of 0.2 mL/min; DAD detection at 340 nm; injection volume of 5.0 μL. The elution gradient was as follows: 0% solvent A (0–2.5 min), 0–5% A (2.5–7.5 min), 5–10% A (7.5–10 min), 10–15% A (10–12.5 min), 15–20% A (12.5–15 min), 20–25% A (15–17.5 min), 25% A (17.5–20 min), 25–60% A (20–27.5 min), 60% A (27.5–30 min), and 60–0% A (30–35 min).Chromatographic analyses were performed using an Acquity UPLC system (Waters Co., MA, USA) equipped with binary solvent delivery system and autosampler. The DAD-UV and mass spectrometer EM Q-TOF Micro-Micromass (Waters Co., MA, USA) detectors were employed, with MassLynx v. 4.1 data acquisition software.The mass spectrometry analyses were performed in positive-ion mode and operated according to the following conditions: collision energy 4.0 eV; temperature of electrospray source and desolvation gas 100°C and 120°C, respectively; capillary voltage 3000 V; sample cone 40 V; extraction cone 3.0 V; N2 was used as the nebulizing. Mass spectra were recorded by using full scan mode in the range ofm/z 200–800.


### 2.6. Hypotensive Activity and Mechanism of Action fromE. falcata Extracts

#### 2.6.1. Animals

Three-month-old male Wistar rats, weighing around 300 g and purchased from the animal house of the Universidade Federal de Santa Maria, were used. They were kept in cages with water availablead libitum, maintaining the temperature controlled at 20–22°C with dark/light cycle of 12 hours.The experimental protocols followed the International Principles for Research Involving Animals (Geneva) (Brazilian legislation by law number 11.794/2008 (procedures for the scientific use of animals), Decree 24.645/34 (animal rights), approved by the Ethics Committee on Animal Use (CEUA), Universidade Federal do Pampa (Protocol 030/2013)).


#### 2.6.2. Hemodynamic Parameters

Rats were anesthetized with urethane (1.4 mg/kg, i.p.) and submitted to surgery for catheterization of the carotid artery (to measure the hemodynamic parameters) and the jugular vein (for administration of the extracts and drugs). Anesthesia was assessed by responsiveness to painful stimuli and when necessary the anesthetic dose was supplemented. For the catheterization, polyethylene catheters (PE 10, Clay-Adams) filled with heparinized saline (50 IU/mL) were used. The arterial catheter was connected to a pressure transducer coupled to an analogue to digital converter (Biopac Systems MP150, Inc.; CA).


#### 2.6.3. Extract Curve

After 30 minutes of stabilization on the equipment, in order to evaluate the effects of the extracts on hemodynamic parameters, a rising curve was performed. 0.5, 2.5, 5, 10, 25, 50, 75, and 100 mg/kg of extract with a volume of 0.2 mL of saline were administered every 10 minutes (n
=
8). Records of systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were made.


#### 2.6.4. Evaluation of Mechanism of Action from Extracts

In order to investigate some possible mechanisms involved in the hypotensive response of the extracts, some drugs were tested after 30 minutes of stabilization: L-NAME, an NO synthesis inhibitor (30 mg/kg,n
=
8); losartan, AT1 receptor antagonist of angiotensin II (10 mg/kg, n
=
8); hexamethonium, ganglionic blocker (20 mg/kg, n
=
8); and propranolol, β-adrenergic blocker (5 mg/kg, n
=
8) [24–27]. After drug administration, a new stabilization period was done and then the extract was administrated (25 mg/kg and 50 mg/kg). Hemodynamic records of SBP, DBP, and HR were then made.


#### 2.6.5. Statistical Analysis

Data were expressed as mean ± standard error of the mean (SEM) and values were analyzed by analysis of variance (ANOVA) followed bypost hoc Tukey. Significance was set at p
<
0
.
05.


## 2.1. Plant Material

Erythrina crista-galli was collected in May 2013 in Uruguaiana, RS (Brazil).Erythrina falcata was collected in Porto Alegre, RS (Brazil), in February 2014. The materials were identified and a voucher specimen of each (Erythrina crista-galli, ICN 183940;Erythrina falcata Benth., ICN 177665) was deposited at the ICN Herbarium (Instituto de Biociências, UFRGS, Avenida Bento Gonçalves, 9500, Prédio 43433, CEP 91501-970, Porto Alegre, RS, Brazil).


## 2.2. Extracts Preparation

Leaves were selected and dried at a temperature of 40°C for five days. After that, they were grounded and submitted to exhaustive extraction processes by maceration using 40% ethanol as solvent (1 : 10, plant : solvent) and by infusion using distilled water at a temperature of 100°C (1 : 15, plant : solvent).For chromatographic assays, extracts obtained by maceration process of both species were used. To reduce the impurities, extracts passed through a liquid-liquid extraction method by affinity solvents with increasing polarity (hexane, dichloromethane, ethyl acetate, and methanol) and for further analysis they were filtered on a membrane of 0.45 micrometers.For biological analyses macerations were used and infusions were obtained fromE. falcata, which were oven-dried and reduced to powder for subsequent redilution in 0.9% saline solution for administration.


## 2.3. Total Phenolic Content

The total phenolic content was determined by Folin-Ciocalteu colorimetric method with some modifications [22]. For the analyses, aliquots of 100 μL of the samples and standard solutions were transferred to test tubes, where 500 μL of Folin-Ciocalteu reagent and 6 mL of distilled water were added. Then, they were agitated and left at rest for 1 minute. After alkalizing the medium, 2 mL of Na2CO3 solution 15% was added, and the volume was completed to 10 mL with distilled water. After 30 minutes at room temperature and being protected from light, samples were analysed in a spectrophotometer Perkin Elmer UV-VIS Lambda 35 (Norwalk, CT, USA) at 750 nm. The total phenolic content was expressed in milligrams of gallic acid equivalent per mL of sample (mgGAE/mL).


## 2.4. Total Flavonoid Content

The total flavonoid content was determined by the method described by Chang et al. [23] with some modifications. In a 25 mL flask 500 μL of sample and 500 μL of AlCl3 0.5% were added and the volume was completed with water. It was incubated for 40 minutes at room temperature and protected from light and reading was done in UV-VIS spectrophotometer Lambda 35 Perkin Elmer (Norwalk, CT, USA) at a wavelength of 415 nm. The results were expressed in mg/g equivalents of rutin.


## 2.5. UPLC-MS

For the protocols a reverse-phase system was performed, using the following conditions: fast C18 analytical column Shim-pack XR-ODS (50 × 2 mm, 2.1μm); mobile phase consisted of acetonitrile : methanol 4 : 1 (v/v) and water with 0.1% formic acid (pH 3.0) with a flow rate of 0.2 mL/min; DAD detection at 340 nm; injection volume of 5.0 μL. The elution gradient was as follows: 0% solvent A (0–2.5 min), 0–5% A (2.5–7.5 min), 5–10% A (7.5–10 min), 10–15% A (10–12.5 min), 15–20% A (12.5–15 min), 20–25% A (15–17.5 min), 25% A (17.5–20 min), 25–60% A (20–27.5 min), 60% A (27.5–30 min), and 60–0% A (30–35 min).Chromatographic analyses were performed using an Acquity UPLC system (Waters Co., MA, USA) equipped with binary solvent delivery system and autosampler. The DAD-UV and mass spectrometer EM Q-TOF Micro-Micromass (Waters Co., MA, USA) detectors were employed, with MassLynx v. 4.1 data acquisition software.The mass spectrometry analyses were performed in positive-ion mode and operated according to the following conditions: collision energy 4.0 eV; temperature of electrospray source and desolvation gas 100°C and 120°C, respectively; capillary voltage 3000 V; sample cone 40 V; extraction cone 3.0 V; N2 was used as the nebulizing. Mass spectra were recorded by using full scan mode in the range ofm/z 200–800.


## 2.6. Hypotensive Activity and Mechanism of Action fromE. falcata Extracts

### 2.6.1. Animals

Three-month-old male Wistar rats, weighing around 300 g and purchased from the animal house of the Universidade Federal de Santa Maria, were used. They were kept in cages with water availablead libitum, maintaining the temperature controlled at 20–22°C with dark/light cycle of 12 hours.The experimental protocols followed the International Principles for Research Involving Animals (Geneva) (Brazilian legislation by law number 11.794/2008 (procedures for the scientific use of animals), Decree 24.645/34 (animal rights), approved by the Ethics Committee on Animal Use (CEUA), Universidade Federal do Pampa (Protocol 030/2013)).


### 2.6.2. Hemodynamic Parameters

Rats were anesthetized with urethane (1.4 mg/kg, i.p.) and submitted to surgery for catheterization of the carotid artery (to measure the hemodynamic parameters) and the jugular vein (for administration of the extracts and drugs). Anesthesia was assessed by responsiveness to painful stimuli and when necessary the anesthetic dose was supplemented. For the catheterization, polyethylene catheters (PE 10, Clay-Adams) filled with heparinized saline (50 IU/mL) were used. The arterial catheter was connected to a pressure transducer coupled to an analogue to digital converter (Biopac Systems MP150, Inc.; CA).


### 2.6.3. Extract Curve

After 30 minutes of stabilization on the equipment, in order to evaluate the effects of the extracts on hemodynamic parameters, a rising curve was performed. 0.5, 2.5, 5, 10, 25, 50, 75, and 100 mg/kg of extract with a volume of 0.2 mL of saline were administered every 10 minutes (n
=
8). Records of systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were made.


### 2.6.4. Evaluation of Mechanism of Action from Extracts

In order to investigate some possible mechanisms involved in the hypotensive response of the extracts, some drugs were tested after 30 minutes of stabilization: L-NAME, an NO synthesis inhibitor (30 mg/kg,n
=
8); losartan, AT1 receptor antagonist of angiotensin II (10 mg/kg, n
=
8); hexamethonium, ganglionic blocker (20 mg/kg, n
=
8); and propranolol, β-adrenergic blocker (5 mg/kg, n
=
8) [24–27]. After drug administration, a new stabilization period was done and then the extract was administrated (25 mg/kg and 50 mg/kg). Hemodynamic records of SBP, DBP, and HR were then made.


### 2.6.5. Statistical Analysis

Data were expressed as mean ± standard error of the mean (SEM) and values were analyzed by analysis of variance (ANOVA) followed bypost hoc Tukey. Significance was set at p
<
0
.
05.


## 2.6.1. Animals

Three-month-old male Wistar rats, weighing around 300 g and purchased from the animal house of the Universidade Federal de Santa Maria, were used. They were kept in cages with water availablead libitum, maintaining the temperature controlled at 20–22°C with dark/light cycle of 12 hours.The experimental protocols followed the International Principles for Research Involving Animals (Geneva) (Brazilian legislation by law number 11.794/2008 (procedures for the scientific use of animals), Decree 24.645/34 (animal rights), approved by the Ethics Committee on Animal Use (CEUA), Universidade Federal do Pampa (Protocol 030/2013)).


## 2.6.2. Hemodynamic Parameters

Rats were anesthetized with urethane (1.4 mg/kg, i.p.) and submitted to surgery for catheterization of the carotid artery (to measure the hemodynamic parameters) and the jugular vein (for administration of the extracts and drugs). Anesthesia was assessed by responsiveness to painful stimuli and when necessary the anesthetic dose was supplemented. For the catheterization, polyethylene catheters (PE 10, Clay-Adams) filled with heparinized saline (50 IU/mL) were used. The arterial catheter was connected to a pressure transducer coupled to an analogue to digital converter (Biopac Systems MP150, Inc.; CA).


## 2.6.3. Extract Curve

After 30 minutes of stabilization on the equipment, in order to evaluate the effects of the extracts on hemodynamic parameters, a rising curve was performed. 0.5, 2.5, 5, 10, 25, 50, 75, and 100 mg/kg of extract with a volume of 0.2 mL of saline were administered every 10 minutes (n
=
8). Records of systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were made.


## 2.6.4. Evaluation of Mechanism of Action from Extracts

In order to investigate some possible mechanisms involved in the hypotensive response of the extracts, some drugs were tested after 30 minutes of stabilization: L-NAME, an NO synthesis inhibitor (30 mg/kg,n
=
8); losartan, AT1 receptor antagonist of angiotensin II (10 mg/kg, n
=
8); hexamethonium, ganglionic blocker (20 mg/kg, n
=
8); and propranolol, β-adrenergic blocker (5 mg/kg, n
=
8) [24–27]. After drug administration, a new stabilization period was done and then the extract was administrated (25 mg/kg and 50 mg/kg). Hemodynamic records of SBP, DBP, and HR were then made.


## 2.6.5. Statistical Analysis

Data were expressed as mean ± standard error of the mean (SEM) and values were analyzed by analysis of variance (ANOVA) followed bypost hoc Tukey. Significance was set at p
<
0
.
05.


## 3. Results and Discussion

### 3.1. Characterization of Plant and Extracts

The water content determination ofE. falcata andE. crista-galli presented values of 64.54% and 63.77%, respectively. The values of total phenolic and total flavonoid content are expressed in Table 1, whose results ranged 0.8771–1.4989 mg GAE/mL and 7.7829–10.4765 mg RE/g, respectively.Table 1
Phenolic and flavonoids content in leaves extracts obtained fromE. falcataand E. crista-galli.
Extraction method
Total phenolic∗ mg GAE/mL (RSD%)
Total flavonoids∗ mgRE/g (RSD%)
E. falcata
Infusion
0.8771 (3.80)
9.3471 (1.27)
Maceration
1.3193 (4.80)
7.7829 (0.85)
E. crista-galli
Infusion
0.9506 (5.09)
10.4765 (1.50)
Maceration
1.4989 (4.29)
8.1976 (1.29)
∗The extracts were analyzed in triplicate.In a study involving the speciesE. indica, the phenolic and flavonoids content were quantified for aqueous and methanolic extracts obtained by maceration [28]. The values were 24.91 and 25.62 mg GAE/g for total phenolic 357.55 and 524.22 mgRE/g for total flavonoids, respectively. Sowndhararajan et al. [29] also evaluated the phenolic content inE. indica for methanolic extracts obtained by maceration of stem, bark, and leaves, demonstrating 412.8 and 184.3 mg GAE/g extract. Another species,E. velutina, was evaluated in terms of total phenolic content in ethanolic and hydroethanolic extracts of seeds, where the levels found were 423.6 and 236.3 mg catechin equivalents/100 g, respectively [30].Our results showed the presence of these chemical classes in the studied medicinal plants, although differences could be observed when compared to published data, probably related to environmental factors, collection locations, experimental procedures (extractive processes), and quantitative values expression.


### 3.2. UPLC-MS

For a better separation profile in the chromatographic run, the analysis was performed by continuous monitoring at 280 and 340 nm, considering as reference for alkaloids and phenolic compounds. In order to improve major peaks, these extracts passed through a purification process by liquid-liquid separation involving increasingly polar solvents (hexane, dichloromethane, ethyl acetate, and methanol). We can observe in Figure1 that the residual ethanol fraction presented a similar profile of separation in the range of 15 to 20 min for both species, when compared to crude extract, although the chemical constituents were detected in major concentrations after purification. Based on these results, the residual ethanol fraction was submitted to analysis by UPLC-MS.Figure 1
Illustrative chromatograms obtained from analysis ofErythrina leaves extracts, with detection at 280 nm. (a)E. falcata: 1: crude hydroethanolic extract at 40%; 2: ethanolic residual fraction. (b)E. crista-galli: 1: crude hydroethanolic extract at 40%; 2: ethanolic residual fraction. (c) UV profile of major chromatographic peak fromE. falcata. (d) UV profile of major chromatographic peak fromE. crista-galli.
(a)
(b)
(c)
(d)The species of the genusErythrina are known for their significant bioproduction of alkaloids, which is the main source of tetracyclicErythrina alkaloids [31]. Based on reported data, a detailed analysis of mass spectra and their respective fragments was performed (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/959081). The careful interpretation was conducted and allowed to suggest the presence of alkaloids derivatives, whose description is illustrated in Table 2. The respective chemical structures are presented in Figure 2.Table 2
Chemical constituents ofE. falcataand E. crista-galli identified by UPLC-MS with correspondent retention times, quasi-molecular ions in positive mode, and key fragments.
Species
Rt (min)
Molecular formula
[M+H]+ (m/z)
MS fragmentation
[M+H]
+ (m/z)
Compound
E. falcata
1
11.72
C20H25O4N
344
316, 299
Erythristemine
2
12.17
C25H33O9N
492
478, 316, 299
11β-Methoxyglucoerysodine
3
12.95
C19H21O7NS
408
348, 316
Erysothiopine
4
13.58
C24H31O9N
478
316, 299
11β-Hydroxyerysodine-glucose
5
13.94
C24H31O9N
478
316, 299
11-Hydroxyerysotinone-rhamnoside
E. crista-galli
1
11.5
C20H25O4N
344
316, 299
Erythristemine
3
12.94
C19H21O7NS
408
349, 332, 316
Erysothiopine
4
13.42
C24H31O9N
478
316, 299
11β-Hydroxyerysodine-glucose
5
13.81
C24H31O9N
478
332, 316, 299
11-Hydroxyerysotinone-rhamnosideFigure 2
Chemical structures of alkaloids suggested for extracts obtained fromE. falcata andE. crista-galli.The presence of these alkaloids has been cited in some works with several species ofErythrina [31–36]. In our study, the presence of the alkaloid erythristemine in both species can be suggested by analyzing the molecular ion [M+H]+ atm/z 344 and its fragments. This alkaloid has been found in the flowers ofE. x bidwillii, a species located in the desert regions [37]. This compound was also reported inE. lysistemon [31]. Juma and Majinda [32] isolated this alkaloid from flowers, pods, and seeds ofE. lysistemon, and also assessed its antioxidant activity.For the molecular ion [M+H]+ atm/z 492 and respective fragments, it is possible to verify a loss of 162 Da, which requires our attention because it is possibly a fragment of hexose [38]. Thus, the presence of the alkaloid 11β-methoxyglucoerysodine is suggested. This derivative has been described inE. lysistemon according to study by Amer et al. [33] that evaluated the composition of the fruits of this species.Equivalent fragments of sugars were also observed in other spectra, suggesting possible alkaloids that may be attached to these fragments as 11β-hydroxyerysodine linked to a hexose which corresponds to 162 Da or even 11-hydroxyerysotinone linked to a rhamnoside fragment, equivalent to 147 Da. These alkaloids, as aglycones, have been described in species ofErythrina. 11β-hydroxyerysodine has been described inE. lysistemon [31] and 11-hydroxyerysotinone inE. macrophylla andE. americana [34, 35].Considering the molecular ion [M+H]+ atm/z 408, the presence of erysothiopine alkaloid is suggested, which was also found inE. glauca seeds [36]. Additionally, this compound is described as one of natural sources of agonists and antagonists of nicotinic acetylcholine receptors [39].


### 3.3. Hemodynamic Parameters

#### 3.3.1. Evaluation ofE. falcata Extract Hemodynamic Curve

According to folk medicine genusErythrina is effective for treatment of hypertension. Ethnobotanical studies also have mentioned the therapeutic use as hypotensive [40–44]. In our study, we consider some literature data that reports the probable hypotensive activity in medicinal speciesE. falcata [40]. Moreover, this species is not frequently explored scientifically, despite its popular use in South of Brazil which indicates a promisorin vivo potential. This context justifies our choice for applyingin vivo protocols only forE. falcata.In the administration curve of infusion and maceration extracts fromE. falcata, an effective and dose-dependent hypotensive action can be observed without affecting the HR (Figure 3). The blood pressure decreased by infusion from doses of 5 mg/kg and 2.5 mg/kg for SBP and DBP, respectively. The maceration extract presented action since the dose of 25 mg/kg for SBP and DBP.Figure 3
Different doses effect ofE. falcata (a) infusion and (b) maceration on hemodynamic parameters in rats. p
∗
<
0
.
05 versus control (n
=
8).
(a)
(b)Another species ofErythrina already showed hypotensive effects. A study by Lehman [20] has shown that alcoholic extracts obtained fromE. americana, when administered intravenously to dogs, rabbits, and rats, caused a decrease in blood pressure from 10 to 20%. Hargreaves et al. [21] also verified the effects ofE. americana, demonstrating that it has similar alkaloids suggested in this work and, between various effects, it showed hypotensive activity.As described in the chemical characterization of the extracts, the content of alkaloids could be contributing on the hypotensive effect. Although alkaloids have a wide range of pharmacological properties, some studies indicated their cardiovascular benefits [45–47]. The erythrinic alkaloids already have many effects described such as antioxidant, anticarcinogenic, anxiolytic, analgesic, and anticonvulsant [48–51]; however, the alkaloids suggested forE. falcata were not well studied pharmacologically; we could expect that these bioactive substances obtained from these genera have important pharmacological potentials.Despite some reports, the present work is the first that describes the hypotensive activity of hydroethanolic and aqueous extracts ofE. falcata, which is extremely important since the incidence of hypertension and use of medicinal plants has been increasing worldwide, generating, thus, future potential phytotherapy and also ensuring the safety of its use by the population.


#### 3.3.2. Evaluation of Mechanism of Action fromE. falcata Extracts

To investigate the possible mechanisms involved in the hypotensive activity of the extracts, different specific drugs, such as L-NAME, losartan, hexamethonium, and propranolol, were administered. L-NAME is an analogue of arginine and competes for NO-synthase generating a prolonged increase in blood pressure and resulting in gross inhibition of NO synthesis [52]. It is widely used in experiments with rats as a model of systemic hypertension [53–57]. After L-NAME, when the infusion extract is administered, SBP, DBP, and HR decreased significantly at both doses (Figure 4(a)). When the maceration extract was administered, SBP and DBP decreased in both doses without changing HR (Figure 4(b)). These data demonstrate that hypotensive action do not have relation to NO pathway, since there was a decrease in blood pressure after administration of L-NAME. Similar results were observed in studies of Kang et al. [54] and Kang et al. [55] that evaluated the effects ofCudrania tricuspidata andFritillaria ussuriensis extracts on blood pressure of L-NAME-induced hypertension rats.Figure 4
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of L-NAME (30 mg/kg). p
∗
<
0.05 versus control and p
#
<
0
.
05 versus L-NAME (n
=
8).
(a)
(b)Losartan causes hypotension through blocking the renin-angiotensin system by selectively antagonizing the receptors for angiotensin II, subtype AT1 [58]. The administration of the extracts, after AT1 receptor blockade, caused a significant decrease in SBP, DBP, and HR at both doses (Figure 5), indicating that this mechanism would not be involved in the hypotensive response. The results of Jiménez-Ferrer et al. [59] differ from our study, but they evaluated the antihypertensive activity ofSalvia elegans extracts compared with losartan as positive control.Figure 5
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of losartan (10 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus losartan (n
=
8).
(a)
(b)The hexamethonium acts on the sympathetic ganglionic blockade, causing decreasing in the blood pressure from arteriolar vasodilatation and cardiovascular reflex blockade [52, 60]. The administration of the extracts after this drug caused a significant reduction in SBP and DBP at both doses. In HR a significant reduction was observed only at 50 mg/kg dose (Figure 6). So this pathway is also not related to the mechanism of action. Study by Suárez et al. [61] evaluatedPimenta dioica extract in SHR rats using hexamethonium. The extract’s hypotensive response decreased significantly with ganglionic blocking, different from our results.Figure 6
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of hexamethonium (20 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus hexamethonium (n
=
8).
(a)
(b)Another drug used was propranolol, which is a potent antagonist nonselective ofβ-adrenergic receptors, acting on β1 and β2 receptors, causing, among other effects, decrease in blood pressure [52]. After the drug, the infusion administration reduced SBP at both doses, but DBP and HR were not decreased in comparison to losartan (Figure 7(a)). Already the administration of maceration extract, SBP, DBP, and HR reduced only in comparison with the control (Figure 7(b)). So we suggest that the hypotensive mechanisms from extracts may be related to the route of β-adrenergic receptors.Figure 7
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of propranolol (5 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus propranolol (n
=
8).
(a)
(b)Nembo et al. [62] investigated the chronotropic effect of aqueous extract ofE. senegalensis and verified that β-adrenergic pathway could be involved in the HR decrease caused by the extract in rats.Other studies with plant extracts also pointedβ-adrenergic pathway as mechanism of its actions for cardiovascular effects such as Praman et al. [63] evaluatingTinospora crispa, which suggested that the hypotensive effect was probably due to the action of the component against β2-adrenergic receptors. Dimo et al. [64] studyingBidens pilosa extracts justified the hypotensive response through the β-adrenergic vasodilation.


## 3.1. Characterization of Plant and Extracts

The water content determination ofE. falcata andE. crista-galli presented values of 64.54% and 63.77%, respectively. The values of total phenolic and total flavonoid content are expressed in Table 1, whose results ranged 0.8771–1.4989 mg GAE/mL and 7.7829–10.4765 mg RE/g, respectively.Table 1
Phenolic and flavonoids content in leaves extracts obtained fromE. falcataand E. crista-galli.
Extraction method
Total phenolic∗ mg GAE/mL (RSD%)
Total flavonoids∗ mgRE/g (RSD%)
E. falcata
Infusion
0.8771 (3.80)
9.3471 (1.27)
Maceration
1.3193 (4.80)
7.7829 (0.85)
E. crista-galli
Infusion
0.9506 (5.09)
10.4765 (1.50)
Maceration
1.4989 (4.29)
8.1976 (1.29)
∗The extracts were analyzed in triplicate.In a study involving the speciesE. indica, the phenolic and flavonoids content were quantified for aqueous and methanolic extracts obtained by maceration [28]. The values were 24.91 and 25.62 mg GAE/g for total phenolic 357.55 and 524.22 mgRE/g for total flavonoids, respectively. Sowndhararajan et al. [29] also evaluated the phenolic content inE. indica for methanolic extracts obtained by maceration of stem, bark, and leaves, demonstrating 412.8 and 184.3 mg GAE/g extract. Another species,E. velutina, was evaluated in terms of total phenolic content in ethanolic and hydroethanolic extracts of seeds, where the levels found were 423.6 and 236.3 mg catechin equivalents/100 g, respectively [30].Our results showed the presence of these chemical classes in the studied medicinal plants, although differences could be observed when compared to published data, probably related to environmental factors, collection locations, experimental procedures (extractive processes), and quantitative values expression.


## 3.2. UPLC-MS

For a better separation profile in the chromatographic run, the analysis was performed by continuous monitoring at 280 and 340 nm, considering as reference for alkaloids and phenolic compounds. In order to improve major peaks, these extracts passed through a purification process by liquid-liquid separation involving increasingly polar solvents (hexane, dichloromethane, ethyl acetate, and methanol). We can observe in Figure1 that the residual ethanol fraction presented a similar profile of separation in the range of 15 to 20 min for both species, when compared to crude extract, although the chemical constituents were detected in major concentrations after purification. Based on these results, the residual ethanol fraction was submitted to analysis by UPLC-MS.Figure 1
Illustrative chromatograms obtained from analysis ofErythrina leaves extracts, with detection at 280 nm. (a)E. falcata: 1: crude hydroethanolic extract at 40%; 2: ethanolic residual fraction. (b)E. crista-galli: 1: crude hydroethanolic extract at 40%; 2: ethanolic residual fraction. (c) UV profile of major chromatographic peak fromE. falcata. (d) UV profile of major chromatographic peak fromE. crista-galli.
(a)
(b)
(c)
(d)The species of the genusErythrina are known for their significant bioproduction of alkaloids, which is the main source of tetracyclicErythrina alkaloids [31]. Based on reported data, a detailed analysis of mass spectra and their respective fragments was performed (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/959081). The careful interpretation was conducted and allowed to suggest the presence of alkaloids derivatives, whose description is illustrated in Table 2. The respective chemical structures are presented in Figure 2.Table 2
Chemical constituents ofE. falcataand E. crista-galli identified by UPLC-MS with correspondent retention times, quasi-molecular ions in positive mode, and key fragments.
Species
Rt (min)
Molecular formula
[M+H]+ (m/z)
MS fragmentation
[M+H]
+ (m/z)
Compound
E. falcata
1
11.72
C20H25O4N
344
316, 299
Erythristemine
2
12.17
C25H33O9N
492
478, 316, 299
11β-Methoxyglucoerysodine
3
12.95
C19H21O7NS
408
348, 316
Erysothiopine
4
13.58
C24H31O9N
478
316, 299
11β-Hydroxyerysodine-glucose
5
13.94
C24H31O9N
478
316, 299
11-Hydroxyerysotinone-rhamnoside
E. crista-galli
1
11.5
C20H25O4N
344
316, 299
Erythristemine
3
12.94
C19H21O7NS
408
349, 332, 316
Erysothiopine
4
13.42
C24H31O9N
478
316, 299
11β-Hydroxyerysodine-glucose
5
13.81
C24H31O9N
478
332, 316, 299
11-Hydroxyerysotinone-rhamnosideFigure 2
Chemical structures of alkaloids suggested for extracts obtained fromE. falcata andE. crista-galli.The presence of these alkaloids has been cited in some works with several species ofErythrina [31–36]. In our study, the presence of the alkaloid erythristemine in both species can be suggested by analyzing the molecular ion [M+H]+ atm/z 344 and its fragments. This alkaloid has been found in the flowers ofE. x bidwillii, a species located in the desert regions [37]. This compound was also reported inE. lysistemon [31]. Juma and Majinda [32] isolated this alkaloid from flowers, pods, and seeds ofE. lysistemon, and also assessed its antioxidant activity.For the molecular ion [M+H]+ atm/z 492 and respective fragments, it is possible to verify a loss of 162 Da, which requires our attention because it is possibly a fragment of hexose [38]. Thus, the presence of the alkaloid 11β-methoxyglucoerysodine is suggested. This derivative has been described inE. lysistemon according to study by Amer et al. [33] that evaluated the composition of the fruits of this species.Equivalent fragments of sugars were also observed in other spectra, suggesting possible alkaloids that may be attached to these fragments as 11β-hydroxyerysodine linked to a hexose which corresponds to 162 Da or even 11-hydroxyerysotinone linked to a rhamnoside fragment, equivalent to 147 Da. These alkaloids, as aglycones, have been described in species ofErythrina. 11β-hydroxyerysodine has been described inE. lysistemon [31] and 11-hydroxyerysotinone inE. macrophylla andE. americana [34, 35].Considering the molecular ion [M+H]+ atm/z 408, the presence of erysothiopine alkaloid is suggested, which was also found inE. glauca seeds [36]. Additionally, this compound is described as one of natural sources of agonists and antagonists of nicotinic acetylcholine receptors [39].


## 3.3. Hemodynamic Parameters

### 3.3.1. Evaluation ofE. falcata Extract Hemodynamic Curve

According to folk medicine genusErythrina is effective for treatment of hypertension. Ethnobotanical studies also have mentioned the therapeutic use as hypotensive [40–44]. In our study, we consider some literature data that reports the probable hypotensive activity in medicinal speciesE. falcata [40]. Moreover, this species is not frequently explored scientifically, despite its popular use in South of Brazil which indicates a promisorin vivo potential. This context justifies our choice for applyingin vivo protocols only forE. falcata.In the administration curve of infusion and maceration extracts fromE. falcata, an effective and dose-dependent hypotensive action can be observed without affecting the HR (Figure 3). The blood pressure decreased by infusion from doses of 5 mg/kg and 2.5 mg/kg for SBP and DBP, respectively. The maceration extract presented action since the dose of 25 mg/kg for SBP and DBP.Figure 3
Different doses effect ofE. falcata (a) infusion and (b) maceration on hemodynamic parameters in rats. p
∗
<
0
.
05 versus control (n
=
8).
(a)
(b)Another species ofErythrina already showed hypotensive effects. A study by Lehman [20] has shown that alcoholic extracts obtained fromE. americana, when administered intravenously to dogs, rabbits, and rats, caused a decrease in blood pressure from 10 to 20%. Hargreaves et al. [21] also verified the effects ofE. americana, demonstrating that it has similar alkaloids suggested in this work and, between various effects, it showed hypotensive activity.As described in the chemical characterization of the extracts, the content of alkaloids could be contributing on the hypotensive effect. Although alkaloids have a wide range of pharmacological properties, some studies indicated their cardiovascular benefits [45–47]. The erythrinic alkaloids already have many effects described such as antioxidant, anticarcinogenic, anxiolytic, analgesic, and anticonvulsant [48–51]; however, the alkaloids suggested forE. falcata were not well studied pharmacologically; we could expect that these bioactive substances obtained from these genera have important pharmacological potentials.Despite some reports, the present work is the first that describes the hypotensive activity of hydroethanolic and aqueous extracts ofE. falcata, which is extremely important since the incidence of hypertension and use of medicinal plants has been increasing worldwide, generating, thus, future potential phytotherapy and also ensuring the safety of its use by the population.


### 3.3.2. Evaluation of Mechanism of Action fromE. falcata Extracts

To investigate the possible mechanisms involved in the hypotensive activity of the extracts, different specific drugs, such as L-NAME, losartan, hexamethonium, and propranolol, were administered. L-NAME is an analogue of arginine and competes for NO-synthase generating a prolonged increase in blood pressure and resulting in gross inhibition of NO synthesis [52]. It is widely used in experiments with rats as a model of systemic hypertension [53–57]. After L-NAME, when the infusion extract is administered, SBP, DBP, and HR decreased significantly at both doses (Figure 4(a)). When the maceration extract was administered, SBP and DBP decreased in both doses without changing HR (Figure 4(b)). These data demonstrate that hypotensive action do not have relation to NO pathway, since there was a decrease in blood pressure after administration of L-NAME. Similar results were observed in studies of Kang et al. [54] and Kang et al. [55] that evaluated the effects ofCudrania tricuspidata andFritillaria ussuriensis extracts on blood pressure of L-NAME-induced hypertension rats.Figure 4
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of L-NAME (30 mg/kg). p
∗
<
0.05 versus control and p
#
<
0
.
05 versus L-NAME (n
=
8).
(a)
(b)Losartan causes hypotension through blocking the renin-angiotensin system by selectively antagonizing the receptors for angiotensin II, subtype AT1 [58]. The administration of the extracts, after AT1 receptor blockade, caused a significant decrease in SBP, DBP, and HR at both doses (Figure 5), indicating that this mechanism would not be involved in the hypotensive response. The results of Jiménez-Ferrer et al. [59] differ from our study, but they evaluated the antihypertensive activity ofSalvia elegans extracts compared with losartan as positive control.Figure 5
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of losartan (10 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus losartan (n
=
8).
(a)
(b)The hexamethonium acts on the sympathetic ganglionic blockade, causing decreasing in the blood pressure from arteriolar vasodilatation and cardiovascular reflex blockade [52, 60]. The administration of the extracts after this drug caused a significant reduction in SBP and DBP at both doses. In HR a significant reduction was observed only at 50 mg/kg dose (Figure 6). So this pathway is also not related to the mechanism of action. Study by Suárez et al. [61] evaluatedPimenta dioica extract in SHR rats using hexamethonium. The extract’s hypotensive response decreased significantly with ganglionic blocking, different from our results.Figure 6
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of hexamethonium (20 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus hexamethonium (n
=
8).
(a)
(b)Another drug used was propranolol, which is a potent antagonist nonselective ofβ-adrenergic receptors, acting on β1 and β2 receptors, causing, among other effects, decrease in blood pressure [52]. After the drug, the infusion administration reduced SBP at both doses, but DBP and HR were not decreased in comparison to losartan (Figure 7(a)). Already the administration of maceration extract, SBP, DBP, and HR reduced only in comparison with the control (Figure 7(b)). So we suggest that the hypotensive mechanisms from extracts may be related to the route of β-adrenergic receptors.Figure 7
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of propranolol (5 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus propranolol (n
=
8).
(a)
(b)Nembo et al. [62] investigated the chronotropic effect of aqueous extract ofE. senegalensis and verified that β-adrenergic pathway could be involved in the HR decrease caused by the extract in rats.Other studies with plant extracts also pointedβ-adrenergic pathway as mechanism of its actions for cardiovascular effects such as Praman et al. [63] evaluatingTinospora crispa, which suggested that the hypotensive effect was probably due to the action of the component against β2-adrenergic receptors. Dimo et al. [64] studyingBidens pilosa extracts justified the hypotensive response through the β-adrenergic vasodilation.


## 3.3.1. Evaluation ofE. falcata Extract Hemodynamic Curve

According to folk medicine genusErythrina is effective for treatment of hypertension. Ethnobotanical studies also have mentioned the therapeutic use as hypotensive [40–44]. In our study, we consider some literature data that reports the probable hypotensive activity in medicinal speciesE. falcata [40]. Moreover, this species is not frequently explored scientifically, despite its popular use in South of Brazil which indicates a promisorin vivo potential. This context justifies our choice for applyingin vivo protocols only forE. falcata.In the administration curve of infusion and maceration extracts fromE. falcata, an effective and dose-dependent hypotensive action can be observed without affecting the HR (Figure 3). The blood pressure decreased by infusion from doses of 5 mg/kg and 2.5 mg/kg for SBP and DBP, respectively. The maceration extract presented action since the dose of 25 mg/kg for SBP and DBP.Figure 3
Different doses effect ofE. falcata (a) infusion and (b) maceration on hemodynamic parameters in rats. p
∗
<
0
.
05 versus control (n
=
8).
(a)
(b)Another species ofErythrina already showed hypotensive effects. A study by Lehman [20] has shown that alcoholic extracts obtained fromE. americana, when administered intravenously to dogs, rabbits, and rats, caused a decrease in blood pressure from 10 to 20%. Hargreaves et al. [21] also verified the effects ofE. americana, demonstrating that it has similar alkaloids suggested in this work and, between various effects, it showed hypotensive activity.As described in the chemical characterization of the extracts, the content of alkaloids could be contributing on the hypotensive effect. Although alkaloids have a wide range of pharmacological properties, some studies indicated their cardiovascular benefits [45–47]. The erythrinic alkaloids already have many effects described such as antioxidant, anticarcinogenic, anxiolytic, analgesic, and anticonvulsant [48–51]; however, the alkaloids suggested forE. falcata were not well studied pharmacologically; we could expect that these bioactive substances obtained from these genera have important pharmacological potentials.Despite some reports, the present work is the first that describes the hypotensive activity of hydroethanolic and aqueous extracts ofE. falcata, which is extremely important since the incidence of hypertension and use of medicinal plants has been increasing worldwide, generating, thus, future potential phytotherapy and also ensuring the safety of its use by the population.


## 3.3.2. Evaluation of Mechanism of Action fromE. falcata Extracts

To investigate the possible mechanisms involved in the hypotensive activity of the extracts, different specific drugs, such as L-NAME, losartan, hexamethonium, and propranolol, were administered. L-NAME is an analogue of arginine and competes for NO-synthase generating a prolonged increase in blood pressure and resulting in gross inhibition of NO synthesis [52]. It is widely used in experiments with rats as a model of systemic hypertension [53–57]. After L-NAME, when the infusion extract is administered, SBP, DBP, and HR decreased significantly at both doses (Figure 4(a)). When the maceration extract was administered, SBP and DBP decreased in both doses without changing HR (Figure 4(b)). These data demonstrate that hypotensive action do not have relation to NO pathway, since there was a decrease in blood pressure after administration of L-NAME. Similar results were observed in studies of Kang et al. [54] and Kang et al. [55] that evaluated the effects ofCudrania tricuspidata andFritillaria ussuriensis extracts on blood pressure of L-NAME-induced hypertension rats.Figure 4
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of L-NAME (30 mg/kg). p
∗
<
0.05 versus control and p
#
<
0
.
05 versus L-NAME (n
=
8).
(a)
(b)Losartan causes hypotension through blocking the renin-angiotensin system by selectively antagonizing the receptors for angiotensin II, subtype AT1 [58]. The administration of the extracts, after AT1 receptor blockade, caused a significant decrease in SBP, DBP, and HR at both doses (Figure 5), indicating that this mechanism would not be involved in the hypotensive response. The results of Jiménez-Ferrer et al. [59] differ from our study, but they evaluated the antihypertensive activity ofSalvia elegans extracts compared with losartan as positive control.Figure 5
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of losartan (10 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus losartan (n
=
8).
(a)
(b)The hexamethonium acts on the sympathetic ganglionic blockade, causing decreasing in the blood pressure from arteriolar vasodilatation and cardiovascular reflex blockade [52, 60]. The administration of the extracts after this drug caused a significant reduction in SBP and DBP at both doses. In HR a significant reduction was observed only at 50 mg/kg dose (Figure 6). So this pathway is also not related to the mechanism of action. Study by Suárez et al. [61] evaluatedPimenta dioica extract in SHR rats using hexamethonium. The extract’s hypotensive response decreased significantly with ganglionic blocking, different from our results.Figure 6
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of hexamethonium (20 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus hexamethonium (n
=
8).
(a)
(b)Another drug used was propranolol, which is a potent antagonist nonselective ofβ-adrenergic receptors, acting on β1 and β2 receptors, causing, among other effects, decrease in blood pressure [52]. After the drug, the infusion administration reduced SBP at both doses, but DBP and HR were not decreased in comparison to losartan (Figure 7(a)). Already the administration of maceration extract, SBP, DBP, and HR reduced only in comparison with the control (Figure 7(b)). So we suggest that the hypotensive mechanisms from extracts may be related to the route of β-adrenergic receptors.Figure 7
Evaluation of hemodynamic parameters in rats treated with doses of 25 mg/kg and 50 mg/kg ofE. falcata (a) infusion and (b) maceration in the presence of propranolol (5 mg/kg). p
∗
<
0.05 versus control and p
#
<
0.05 versus propranolol (n
=
8).
(a)
(b)Nembo et al. [62] investigated the chronotropic effect of aqueous extract ofE. senegalensis and verified that β-adrenergic pathway could be involved in the HR decrease caused by the extract in rats.Other studies with plant extracts also pointedβ-adrenergic pathway as mechanism of its actions for cardiovascular effects such as Praman et al. [63] evaluatingTinospora crispa, which suggested that the hypotensive effect was probably due to the action of the component against β2-adrenergic receptors. Dimo et al. [64] studyingBidens pilosa extracts justified the hypotensive response through the β-adrenergic vasodilation.


## 4. Conclusions

The chemical composition ofE. falcata andE. crista-galli species is based on alkaloids. Through the UPLC-MS analysis the presence of five different alkaloids can be suggested for the studied species, namely, erythristemine, 11β-methoxyglucoerysodine, erysothiopine, 11β-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhanmoside. The hydroethanolic and aqueous extracts ofE. falcata show to possessin vivo dose-dependent hypotensive activity, which could be associated with the alkaloids content. Besides, the mechanism of this effect may be related to the route of β-adrenergic receptors.



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*Source: 959081-2015-08-18.xml*