# Changes in the Physicochemical Quality Characteristics of Cabbage Kimchi with respect to Storage Conditions

Authors: Su-Yeon You; Ji-Su Yang; Sung Hyun Kim; In Min Hwang
Journal: Journal of Food Quality
 (2017)

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## Abstract

In this study, the changes in pH, organic acid content, acidity, and salinity of kimchi prepared at 0 days, stored 1–8 weeks at 4 and 10°C, and stored at room temperature for two days were analyzed. Organic acids content was analyzed by using analytical technique of high-performance liquid chromatography (HPLC). This method was also validated using quality assurance parameters of linearity, limits of detection and quantification (LOD and LOQ), precision, and spike recovery experiments. In the analysis of organic acids content (mg/kg), it was found that the kimchi stored for 1–8 weeks at 4 and 10°C showed gradual increase in the organic acids content during storage period. The order of organic acids was lactic acid > acetic acid > citric acid > malic acid > succinic acid > oxalic acid > fumaric acid. The pH values of kimchi stored at 4°C, 10°C, and 25°C were 4.1, 3.6–3.7, and 4.1, respectively.


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## Body

## 1. Introduction

Kimchi is a traditional fermented food item and side dish with a unique flavor in Korea consumed for over a long time period. Now, it has been consumed as a health food in different countries. The main ingredients of kimchi are cabbage and radish. However, before fermentation various spices such as chili powder, ginger, green onion, garlic, and salted seafood are also added to it. The organic acids and free amino acids generated during fermentation render kimchi its unique flavor. Chinese cabbage kimchi (Baechu kimchi) has unique flavors which vary with the ingredients as well as ripening and distribution [1]. In the past, it had been in houses only. However, due to the changes in the diets of modern society following socioeconomic development, currently, it is produced by companies on a large scale [2]. Segmentation of the kimchi market owing to expansion of the commercial kimchi market and diversification of kimchi preference by consumers has resulted in a growing demand for kimchi of varied ripeness [3].During the fermentation of kimchi, the content of organic acids increases and that of sugar decreases. Concerning the pH, it decreases with fermentation which directly and indirectly affects the sensory characteristics of kimchi [4]. Previous studies on the fermentation characteristics of kimchi indicated that the flavor of kimchi is significantly affected by fermentation, ripening and distribution. Lee and Chun [5] reported that its shelf life when fermented at 4°C and 28°C is 30 and 3 days, respectively. Furthermore, another study reported that kimchi stored at 17°C for 4 days and that stored at 4°C for 48 days have similar quality characteristics. Also, changes in pH, acidity, and organic acid content were reported by the researchers [5, 6].Organic acids are produced by the growth of lactobacillus during fermentation and ripening of kimchi by converting the fermentable sugar contained in vegetables to lactic acid or other organic acids [2]. Thus, ripening and acidification of kimchi directly affect the organic acid production, and sour and savory flavors harmonize at an optimum fermentation time [7]. There are several studies on changes of quality during the fermentation and the factors affecting the flavor and quality of kimchi [8]. The nonvolatile organic acids in kimchi with respect to ripening temperature [9], changes of organic acids and volatile flavor components of kimchi with respect to types of ingredients [10], changes of biological and chemical properties of over-ripened kimchi [11], and effects of the combination of fermentation temperature and time on the quality characteristics of cabbage kimchi [12] have been also previously investigated.Because of the varied ingredients and fermentation environment, kimchi varies significantly; therefore, it is difficult to analyze its characteristics. There are very few studies on changes of acidity or organic acids content with respect to specific conditions during kimchi fermentation. Low-temperature/refrigeration temperature fermentation and storage is widely employed for kimchi ripening. The studies conducted thus far indicate that total acidity, which is an indirect quality index, is highly affected by ripening temperature which in turn significantly affects the fermentation speed of kimchi [8, 13]. In addition, fermentation patterns are more strongly associated with temperature than salinity as the bacteria involved vary with the fermentation temperature, resulting in the changes in kimchi flavor [12]. The time required to reach pH 4.2, which is the pH for the optimal flavor of kimchi, is 10 days at 4°C, 2.4 days at 15°C, 1 day at 25°C, and 19 h at 35°C [8].The current study was aimed at analyzing the changes in quality attributes such as pH, organic acids content, acidity, and salinity of the kimchi stored at different temperatures and time, which contribute to kimchi flavour.


## 2. Materials and Methods

### 2.1. Samples

In this study, cabbage kimchi samples used were from summer cabbage grown in Taebaek, Korea, in 2015. All the raw materials used for preparing samples were purchased from Western Agricultural Market in Gwangju Metropolitan City, Korea. The minor ingredients such as chili powder and fermented anchovy sauce required for making kimchi were purchased from market.


### 2.2. Kimchi Production and Storage Conditions

Cabbage and additional minor ingredients were preprocessed on the day of purchase so that kimchi could be made the following day. Cabbages were sliced in half after the removal of nonedible parts. Subsequently, the cabbage pieces were salted using the salt water soaking method at room temperature (25°C) for 12 h in salt water with a salinity of 10% [4]. For homogeneous salting, water baskets were used to soak all cabbages and after 2-3 h the cabbage in the upper and lower positions were switched. After 18 h, salted cabbages were washed 3 times and water was drained for 2 h. Then, a prepared seasoning consisting of radish, green onion, garlic, ginger, fermented anchovy sauce, chili powder, and glutinous rice paste was added to make kimchi. The prepared kimchi sample was taken at 0 days and also divided into batches to be stored and fermented for 1–8 weeks at 4, 10, and 25°C which were then fermented at each temperature. The quality characteristics of kimchi were analyzed at each week from 1–8 weeks at 4°C and 10°C, and the kimchi stored at room temperature was rapidly ripened for 2 days before its use in the experiment.


### 2.3. Measurement of pH and Acidity

Each 500 g of kimchi stored under each condition was homogenized in a blender (HR1372, 700 W; Philips, Amsterdam, Netherlands) for measuring the pH and acidity of each sample. The solid contents were filtered through sterilized gauze and the filtrate was used for subsequent measurements. The pH and acidity were both measured using a pH meter (TitroLine 5000; SI Analytics, Mainz Germany). The pH was measured by immersing a pH electrode of the pH meter in kimchi liquid. For measuring acidity by the Association of Official Analytical Chemists (AOAC) method [14], the amount of 0.1 N NaOH required to neutralize 10 mL of kimchi liquid to pH 8.3 was used. It was converted to lactic acid content (%) and recorded as the titratable acidity. Each experiment was conducted in triplicate for obtaining mean and standard deviation.


### 2.4. Measurement of Salinity

For measuring salinity, 1 g of each homogenized kimchi sample was added to a 100 mL flask and adjusted to a constant volume with distilled water. Then, 100 mL well-mixed sample solution was filtered in a 100 mL Erlenmeyer flask and the filtrate was used for measurements. Using the Mohr method [15] to measure salinity, 1 mL of 2% potassium chromate was added to 10 mL of the filtrate and 0.02 N AgNO3 was used for titration. The amount of 0.02 N AgNO3 used to change the color to red was observed.


### 2.5. Organic Acid Content Analysis

Each 500 g of kimchi stored under each condition was homogenized in a blender for measuring the organic acid content of the samples. Then, 12.5 mL of distilled water was added to 2 g of sample. The organic acids were extracted for 30 min using a sonicator (Power Sonic 520; Hwashin Tech Co., Daegu, Korea). After extraction, the sample extract adjusted to 25 mL was filtered using a filter paper. This filtrate was then filtered using a syringe filter (RC, 0.2μm, 25 mm) and used for high-performance liquid chromatography (1260 Infinity/G4212B; Agilent Co., Santa Clara, CA, USA) analysis. Oxalic acid, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, fumaric acid, and acetic acid (Sigma, St. Louis, Missouri, USA), were used as standards for organic acids analysis. The standards and the samples were analyzed by HPLC using the conditions set by [16] which are given in Table 1. The organic acids detected in the kimchi samples were identified by comparing the retention times with those of the standard organic acids. Then, the organic acids in each sample were quantitatively analyzed using a calibration curve derived from the peak areas of the standard organic acids.Table 1
HPLC analysis operating conditions and parameters for determination of organic acids.
HPLC Agilent 1260 Infinity/G4212B 1260 DAD Column Aminex HP × 87H column (7.8 mm × 300 mm, 9µm, Bio-Rad, Hercules, CA, USA) Mobile phase 0.008 N H2SO4 Detector DAD, 210 nm Injection volume 10µL Flow rate 0.6 mL/min


### 2.6. Quality Assurance

The analytical method for the determination of the organic acids in kimchi was validated by evaluating several quality control parameters such as linearity, limits of detection (LOD) and limits of quantification (LOQ), precision, and accuracy (spike recovery%). Linearity in terms of correlation coefficient (R2) values was determined from the calibration curves [17, 18]. The LOD and LOQ were calculated as three and ten times of the standard deviations of the blank divided by the slope of the analytical curve, respectively [19]. The precision was determined by evaluating the coefficient of variation (CV%) using the relative standard deviation of ten repeated determinations of one sample [20]. The analytical quality control was verified by the recovery experiments spiking at selected concentration in the samples.


### 2.7. Statistical Analysis

Each experiment was repeated three or more times and presented as mean ± standard deviation. To test the significance of the results, the SPSS Version 8.2 software (SPSS Institute Inc., Cary, NC, USA) was used. Duncan’s multiple range test was conducted atp<0.05. The difference in the organic acid content of kimchi with respect to storage temperature was evaluated by linear discriminant analysis (LDA). LDA is a nonparametric test that assumes that various classes of similar objects are described by multivariate normal distributions with the same covariance. LDA was performed using the XLSTAT 2015 program (Addinsoft, Paris, France) [21].


## 2.1. Samples

In this study, cabbage kimchi samples used were from summer cabbage grown in Taebaek, Korea, in 2015. All the raw materials used for preparing samples were purchased from Western Agricultural Market in Gwangju Metropolitan City, Korea. The minor ingredients such as chili powder and fermented anchovy sauce required for making kimchi were purchased from market.


## 2.2. Kimchi Production and Storage Conditions

Cabbage and additional minor ingredients were preprocessed on the day of purchase so that kimchi could be made the following day. Cabbages were sliced in half after the removal of nonedible parts. Subsequently, the cabbage pieces were salted using the salt water soaking method at room temperature (25°C) for 12 h in salt water with a salinity of 10% [4]. For homogeneous salting, water baskets were used to soak all cabbages and after 2-3 h the cabbage in the upper and lower positions were switched. After 18 h, salted cabbages were washed 3 times and water was drained for 2 h. Then, a prepared seasoning consisting of radish, green onion, garlic, ginger, fermented anchovy sauce, chili powder, and glutinous rice paste was added to make kimchi. The prepared kimchi sample was taken at 0 days and also divided into batches to be stored and fermented for 1–8 weeks at 4, 10, and 25°C which were then fermented at each temperature. The quality characteristics of kimchi were analyzed at each week from 1–8 weeks at 4°C and 10°C, and the kimchi stored at room temperature was rapidly ripened for 2 days before its use in the experiment.


## 2.3. Measurement of pH and Acidity

Each 500 g of kimchi stored under each condition was homogenized in a blender (HR1372, 700 W; Philips, Amsterdam, Netherlands) for measuring the pH and acidity of each sample. The solid contents were filtered through sterilized gauze and the filtrate was used for subsequent measurements. The pH and acidity were both measured using a pH meter (TitroLine 5000; SI Analytics, Mainz Germany). The pH was measured by immersing a pH electrode of the pH meter in kimchi liquid. For measuring acidity by the Association of Official Analytical Chemists (AOAC) method [14], the amount of 0.1 N NaOH required to neutralize 10 mL of kimchi liquid to pH 8.3 was used. It was converted to lactic acid content (%) and recorded as the titratable acidity. Each experiment was conducted in triplicate for obtaining mean and standard deviation.


## 2.4. Measurement of Salinity

For measuring salinity, 1 g of each homogenized kimchi sample was added to a 100 mL flask and adjusted to a constant volume with distilled water. Then, 100 mL well-mixed sample solution was filtered in a 100 mL Erlenmeyer flask and the filtrate was used for measurements. Using the Mohr method [15] to measure salinity, 1 mL of 2% potassium chromate was added to 10 mL of the filtrate and 0.02 N AgNO3 was used for titration. The amount of 0.02 N AgNO3 used to change the color to red was observed.


## 2.5. Organic Acid Content Analysis

Each 500 g of kimchi stored under each condition was homogenized in a blender for measuring the organic acid content of the samples. Then, 12.5 mL of distilled water was added to 2 g of sample. The organic acids were extracted for 30 min using a sonicator (Power Sonic 520; Hwashin Tech Co., Daegu, Korea). After extraction, the sample extract adjusted to 25 mL was filtered using a filter paper. This filtrate was then filtered using a syringe filter (RC, 0.2μm, 25 mm) and used for high-performance liquid chromatography (1260 Infinity/G4212B; Agilent Co., Santa Clara, CA, USA) analysis. Oxalic acid, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, fumaric acid, and acetic acid (Sigma, St. Louis, Missouri, USA), were used as standards for organic acids analysis. The standards and the samples were analyzed by HPLC using the conditions set by [16] which are given in Table 1. The organic acids detected in the kimchi samples were identified by comparing the retention times with those of the standard organic acids. Then, the organic acids in each sample were quantitatively analyzed using a calibration curve derived from the peak areas of the standard organic acids.Table 1
HPLC analysis operating conditions and parameters for determination of organic acids.
HPLC Agilent 1260 Infinity/G4212B 1260 DAD Column Aminex HP × 87H column (7.8 mm × 300 mm, 9µm, Bio-Rad, Hercules, CA, USA) Mobile phase 0.008 N H2SO4 Detector DAD, 210 nm Injection volume 10µL Flow rate 0.6 mL/min


## 2.6. Quality Assurance

The analytical method for the determination of the organic acids in kimchi was validated by evaluating several quality control parameters such as linearity, limits of detection (LOD) and limits of quantification (LOQ), precision, and accuracy (spike recovery%). Linearity in terms of correlation coefficient (R2) values was determined from the calibration curves [17, 18]. The LOD and LOQ were calculated as three and ten times of the standard deviations of the blank divided by the slope of the analytical curve, respectively [19]. The precision was determined by evaluating the coefficient of variation (CV%) using the relative standard deviation of ten repeated determinations of one sample [20]. The analytical quality control was verified by the recovery experiments spiking at selected concentration in the samples.


## 2.7. Statistical Analysis

Each experiment was repeated three or more times and presented as mean ± standard deviation. To test the significance of the results, the SPSS Version 8.2 software (SPSS Institute Inc., Cary, NC, USA) was used. Duncan’s multiple range test was conducted atp<0.05. The difference in the organic acid content of kimchi with respect to storage temperature was evaluated by linear discriminant analysis (LDA). LDA is a nonparametric test that assumes that various classes of similar objects are described by multivariate normal distributions with the same covariance. LDA was performed using the XLSTAT 2015 program (Addinsoft, Paris, France) [21].


## 3. Results and Discussion

### 3.1. Validation of Analytical Method

In validation of the HPLC analytical method, the values of LOD were in the range of 0.806–4.447 (mg/kg) and the LOQ values ranged from 2.442 to 13.475 (mg/kg) (Table2). The recovery (%) was found to be in the range of 90.12–109.81%. The CV% values obtained were all less than 3% (0.37–1.99%). Thus, the results obtained for the quality assurance parameters indicated that the applied analytical method is fully validated and fulfils the criteria required by AOAC.Table 2
Analytical parameters for HPLC analysis of organic acids.
Organic acid Correlation coefficient (R2) LOD1 (mg/kg) LOQ2 (mg/kg) Recovery3 (%) CV4 (%) Oxalic acid 0.99986 4.447 13.475 97.55 0.53 Citric acid 0.99978 1.136 3.442 91.60 0.54 Tartaric acid 0.99985 1.759 5.329 100.99 0.37 Malic acid 0.99972 1.683 5.101 107.21 1.29 Succinic acid 0.99955 1.321 4.003 109.81 1.26 Lactic acid 0.99958 1.503 4.555 93.35 0.81 Fumaric acid 0.99915 1.309 3.967 90.12 0.72 Acetic acid 0.99880 0.806 2.442 97.55 1.99 1 L i m i t of detection. 2Limit of quantification. 3Recovery of citric acid, malic acid, succinic acid, lactic acid, and acetic acid spiked at 1,000 ppm, oxalic acid spiked at 200 ppm, tartaric acid spiked at 500 ppm, and fumaric acid spiked at 5 ppm. 4Coefficient of variation.


### 3.2. Changes of pH during Storage

During the fermentation of kimchi, major components contained in Chinese cabbage and minor ingredients (salted seafood, chili powder, green onion, garlic, and ginger) are degraded or synthesized by microorganisms using various enzymes. The degradation of carbohydrates, a major component of Chinese cabbage, results in the formation of numerous organic acids which gives unique flavor to kimchi [5, 22, 23]. Thus, the pH, total acidity and organic acid of kimchi are important quality indices for determining the quality and storage level of kimchi. The pH changes in kimchi at 0 days and stored at 4 and 10°C for 8 weeks are summarized in Table 3. From the results, it can be noticed that the kimchi stored at 25°C for 2 days exhibited a pH of 4.1 and that at 0 days it exhibited a pH of 5.3. However, the one stored at 4°C showed a pH change ranged from 5.3 to 5.8 during 1–3 weeks which did not change considerably during early storage. However, as weeks passed, the pH gradually decreased to 4.1. The pH of kimchi stored at 10°C for 8 weeks exhibited an early rapid decrease from 5.8 at 0 days to 4.7 at 1 week. It gradually decreased until 6 weeks until it remained constant at 3.6–3.7. The pH values of the kimchi stored at 25°C were 5.3 at 0 day and 4.1±0.1 at 2 days. In general, the pH changes in kimchi at each temperature during fermentation exhibit a sigmoid curve: a slow decrease at the initial fermentation until it reaches 5.4 as fermentation progresses, rapid decrease to 4.2–4.4 at intermediate fermentation stage (the optimal fermentation stage), and a decrease to 4.0 at the final fermentation stage [8]. Such sigmoid curve is observed for the growth of living organisms which in case of kimchi is attributed to bacteria. In this study, the initial fermentation stage for kimchi stored at 4°C was observed until three weeks and the intermediate fermentation stage was observed over three weeks until the pH reached 4.1. However, kimchi stored at 10°C exhibited a very short initial fermentation stage and a rapid pH decrease in the intermediate fermentation stage. From 6 to 8 weeks when pH was 3.7, the final fermentation stage was observed with no changes in pH values. The kimchi produced in summer and stored at 4 and 10°C exhibited different trends in pH with storage over 8 weeks. The results suggest that low-temperature storage at 4°C allows a slower fermentation rate and permits long-term fermentation, whereas kimchi stored at 10°C exhibits high initial fermentation, which slows down once a certain fermentation level is reached. It further indicates that pH changes are more highly associated with temperature rather than salinity, implying that fermentation temperature largely affects the flavor of kimchi [24].Table 3
pH, acidity, and salinity of kimchi samples stored at 4 and 10°C1,2.
Storage period pH Acidity (%) Salinity (%) 0 days 5.84 ± 0.01 c 0.25 ± 0.00 p 2.03 ± 0.00 i  Storage period (weeks) and temperature (4°C) 1 5.76 ± 0.01 a 0.27 ± 0.01 q 1.84 ± 0.01 k 2 5.52 ± 0.01 b 0.36 ± 0.00 o 2.10 ± 0.01 h 3 5.31 ± 0.01 d 0.38 ± 0.01 n 2.28 ± 0.01 d 4 5.07 ± 0.00 e 0.42 ± 0.01 m 2.15 ± 0.01 g 5 4.90 ± 0.01 f 0.43 ± 0.01 l 2.23 ± 0.01 f 6 4.74 ± 0.00 g 0.53 ± 0.01 j 2.35 ± 0.01 b 7 4.27 ± 0.00 j 0.69 ± 0.02 h 2.52 ± 0.01 a 8 4.12 ± 0.00 k 0.79 ± 0.01 g 2.22 ± 0.02 f  Storage period (weeks) and temperature (10°C) 1 4.68 ± 0.01 h 0.51 ± 0.01 k 2.15 ± 0.02 g 2 4.33 ± 0.01 i 0.66 ± 0.01 i 2.14 ± 0.01 g 3 3.96 ± 0.01 l 1.01 ± 0.02 f 2.22 ± 0.01 f 4 3.85 ± 0.04 m 1.22 ± 0.02 e 2.32 ± 0.01 c 5 3.76 ± 0.01 n 1.26 ± 0.02 d 1.99 ± 0.00 j 6 3.70 ± 0.00 o 1.51 ± 0.02 b 1.81 ± 0.02 l 7 3.64 ± 0.00 q 1.48 ± 0.00 c 2.53 ± 0.01 a 8 3.68 ± 0.01 p 1.57 ± 0.01 a 2.25 ± 0.01 e  Storage period (days) and temperature (25°C) 2 4.1 ± 0.1 0.91 ± 0.1 2.22 ± 0.1 1Each value is mean ± SD (n=3). 2Means with the different superscript letters in the same column are significantly different (p<0.05).


### 3.3. Changes of Acidity during Storage

The results of acidity changes in kimchi, stored at 4 and 10°C for 8 weeks, are shown in Table3. The results showed that kimchi stored at 4°C showed no considerable acidity changes (0.3–0.4% from 0 day to 5 weeks). In contrast, the acidity of kimchi stored at 10°C increased from the early storage stage showing 0.3–1.57% increase from 0 days to 8 weeks. The acidity of kimchi stored at 10°C exhibited the initial, intermediate, and final stages in the same manner as that observed for pH changes, whereas that stored at 4°C showed a gradual increase in acidity. As a result, the acidity of kimchi stored at 10°C was 1.57% at 8 weeks, which was two times that of kimchi stored at 4°C (0.79%). The acidity of kimchi of 25 storage temperature exhibited the acidity of 0.25% and 0.9% at 0 and 2 days, respectively. During the fermentation of kimchi, various organic acids are generated by the reproduction of microorganisms which lower the pH and increase the acidity of kimchi [8]. An increase of total acidity and a decrease of pH have been reported to be significantly affected by microorganisms, sugar content, salt concentration, and temperature. Generally, the pH decline is a gradual decrease compared with the increase of total acidity, which is assumed to be caused by the buffer reaction of proteins and amino acids in kimchi [25]. The acidity does not increase significantly because aerobic microorganisms unassociated with acid production are temporarily active during the early ripening of kimchi, lactobacillus remains inactive and also because of water being released from Chinese cabbage [26]. In our study, the acidity of kimchi stored at 4°C gradually increased from 5 weeks, whereas kimchi stored at 10°C increased rapidly from 0 days to 5 weeks. These changes in acidity are significantly affected by ripening temperature and saline concentration as lactobacillus start to grow after their inactive period. From the previous studies, it was found that it took 2 days to reach full ripening at a salinity of 3% and a storage temperature of 20°C, 1 day at a salinity of 2.25%–3.50% and a storage temperature of 30°C, and 2–4 days at a salinity of 5% and a storage temperature of 30°C [27]. Considering that optimal fermentation occurs at an acidity and pH of 0.6% and 4.2, respectively, in the current study, kimchi ripened for 7 weeks at a storage temperature of 4°C exhibited a pH of 4.3 and acidity of 0.7%, whereas kimchi ripened for 2 weeks at a storage temperature of 10°C exhibited a pH of 4.3 and acidity of 0.7%. These optimal fermentation times were determined to be the time of full ripening above these conditions.


### 3.4. Changes of Salinity during Storage

In this study, the kimchi prepared at 0 day and that stored at 4 and 10°C were analyzed for the salinity differences. From the results given in Table3, the salt content ranged from 1.81% to 2.35%, suggesting that the salinity of kimchi did not change considerably at each storage time and temperature. The salinity of kimchi fermented at 25°C was found to be 2.03% and 2.22% at 0 and 2 days, respectively. From the previous literature, during the fermentation of kimchi with added abalone and kelp, the initial salinity was 1.37–1.67% and maintained at 2% because of the kimchi liquid and tissues of major and minor ingredients [28]. In another study, the kimchi made from summer Chinese cabbage of the alpine region, the salinity values of samples stored at 15°C and 5°C were 2.51–2.58 and 2.50–2.58, respectively, whereas measuring by the Mohr method has given slightly lower values of 2.41–2.48 [2]. Thus, several studies proved that salinity does not inhibit the growth of microorganisms for the fermentation of kimchi.


### 3.5. Changes in Organic Acids Content

In this study, a total of eight organic acids including oxalic acid, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, fumaric acid, and acetic acid were identified. The results of organic acids content of kimchi by various storage conditions are summarized in Table4. The results showed that the kimchi stored at 25°C for 2 days contained only oxalic acid, lactic acid, and acetic acid with the content levels (mg/kg) of 76.2±0.6, 4296.3±78.6, and 2913.3±6.9. As shown in Figure S1 (A) in Supplementary Material available online at https://doi.org/10.1155/2017/9562981, the kimchi stored at 4°C ripened for 8 weeks, malic acid exhibited the highest content during the early storage, and lactic acid exhibited the highest content at 8th week. The kimchi stored at 10°C ripened for 8 weeks, lactic acid content was markedly high at the early storage until 8 weeks followed by acetic acid content (Figure S1 (B)). Previous study reported that citric acid and malic acid gradually decrease as ripening progressed whereas lactic acid and acetic acid rapidly increase. Lactic acid was confirmed to be the main organic acid at the last stage of ripening [29]. Other study on kimchi added with abalone and kelp reported that citric acid, malic acid, and succinic acid contents were high during the initial fermentation stage and then gradually decreased as fermentation progressed, with the lactic acid and acetic acid contents increasing on days 7 and 14 of fermentation, respectively [28]. Furthermore, another study revealed that, after ripening of kimchi for 8 weeks at 5°C, the lactic acid which was low during the early storage exhibited a marked increase from day 13 of storage, whereas malic acid exhibited a decrease and the succinic acid, oxalic acid, and malonic acid contents did not change [30].Table 4
Organic acids content (mg/kg) in kimchi samples stored at 4 and 10°C1,2.
Storage period Oxalic acid Citric acid Tartaric acid Malic acid Succinic acid Lactic acid Fumaric acid Acetic acid 16.0 ± 0.5 1 , 362.3 ± 84.0 ND 1 , 546.6 ± 13.0 ND ND 11.5 ± 0.1 273.4 ± 9.8  Storage period (weeks) and temperature (4°C) 1 21.9 ± 0.8 e 1 , 565.0 ± 5.0 a ND 1 , 183.8 ± 14.6 b ND N D g 11.1 ± 0.1 a 279.7 ± 5.3 h 2 29.3 ± 0.7 c 1 , 250.6 ± 2.2 b ND 1 , 469.8 ± 32.6 a ND 188.1 ± 1.6 f 9.8 ± 0.1 b 391.4 ± 8.0 g 3 33.0 ± 1.1 b 1 , 220.9 ± 9.9 b c ND 906.5 ± 4.4 d ND 296.1 ± 6.9 e 9.6 ± 0.0 c 460.5 ± 9.4 e 4 29.0 ± 0.9 c 1 , 129.7 ± 10.6 d ND 1 , 046.8 ± 17.8 c ND 292.8 ± 4.9 e 8.0 ± 0.0 d 437.9 ± 9.4 f 5 25.7 ± 0.7 d 1 , 189.1 ± 44.2 c ND 770.8 ± 10.6 e ND 1 , 013.9 ± 67.1 d 7.9 ± 0.0 d 476.9 ± 12.0 d 6 36.6 ± 1.7 a 1 , 069.2 ± 11.5 e ND 305.0 ± 10.5 f ND 2 , 005.1 ± 12.4 c 4.9 ± 0.0 g 649.2 ± 2.9 c 7 29.1 ± 0.4 c 872.5 ± 35.4 f ND N D g ND 4 , 149.7 ± 24.2 b 6.5 ± 0.1 e 698.3 ± 2.1 b 8 31.5 ± 0.9 b 751.7 ± 1.9 g ND N D g ND 5 , 194.9 ± 32.5 a 5.7 ± 0.0 f 821.6 ± 8.7 a  Storage period (weeks) and temperature (10°C) 1 31.7 ± 1.4 c 576.6 ± 60.1 a ND 434.3 ± 15.6 a N D c 2 , 556.3 ± 32.7 h 6.4 ± 0.1 a 935.8 ± 14.6 f 2 24.9 ± 0.5 d 101.7 ± 0.9 b ND 229.6 ± 4.0 b N D c 3 , 676.4 ± 4.3 g 1.6 ± 0.0 b 1 , 378.3 ± 18.6 e 3 30.6 ± 0.7 c N D c ND N D c 365.3 ± 6.7 b 6 , 100.4 ± 45.8 f N D c 2 , 302.1 ± 51.2 d 4 34.8 ± 2.2 b N D c ND N D c 421.9 ± 18.7 a 7 , 459.9 ± 51.9 e N D c 2 , 710.6 ± 41.0 c 5 22.9 ± 0.7 d N D c ND N D c 343.3 ± 3.8 b 7 , 842.2 ± 161.3 d N D c 2 , 832.4 ± 81.5 b 6 35.2 ± 1.6 b N D c ND N D c 418.4 ± 19.4 a 11 , 922.4 ± 59.7 b N D c 2 , 978.1 ± 43.3 a 7 35.6 ± 1.6 b N D c ND N D c 343.4 ± 12.9 b 11 , 766.2 ± 41.3 c N D c 3 , 031.8 ± 25.3 a 8 38.3 ± 1.9 a N D c ND N D c 361.9 ± 4.6 b 12 , 621.2 ± 68.5 a N D c 2 , 800.9 ± 54.1 b  Storage period (days) and temperature (25°C) 2 76.2 ± 0.6 ND ND ND ND 4296.3 ± 48.6 ND 2913.3 ± 6.9 1Each value is mean ± SD (n=3). 2Means with the different superscript letters in the same column are significantly different (p<0.05).With regard to the present study, in case of kimchi stored at 4°C, the malic acid content was gradually decreased upon ripening and lactic acid content, which was not detected during early storage, rapidly increased at 5th week of storage. The kimchi stored at 10°C, lactic acid content was the highest from the early storage and its rate of increase became higher with increasing storage time. Malic acid content was not detected up to 2 weeks in the kimchi stored at 10°C and 25°C. Similarly, the malic acid content in kimchi stored at 4°C and 10°C rapidly decreased and was not detected at 6th and 4th week. The decrease in malic acid is reported to be caused by the conversion of malic acid to lactic acid and acetic acid by lactobacillus in kimchi [11]. The oxalic acid content did not change significantly with the storage period in kimchi stored at 4°C and 10°C, and it was two times higher in kimchi stored at 25°C. Fumaric acid had the lowest content (1.6–11.1 mg/kg).When changes of the organic acid contents were compared, it was found that the changes in acidity are mainly dependent on lactic acid content. The studies showed that kimchi exhibit a pH of 4.2–4.6 [8], acidity of 0.6–0.75% [16], and lactic acid content of 0.5–0.75% at the optimal fermentation time [31]. In the current study, kimchi stored at 4°C for 7 weeks and 10°C for 2 weeks was found to be the optimally fermented kimchi (Table 3). The lactic acid content in kimchi stored at 4°C for 7 weeks and that stored at 10°C for 2 weeks was 0.4% and 0.36%, respectively, lower than those reported by Kim (1985) [31]. The lactic acid content in kimchi stored at 4°C for 8 weeks and that stored at 10°C for 3 weeks was found to be 0.5% and 0.6%, respectively. Therefore, the optimal fermentation time for kimchi derived from lactic acid content appeared 1 week later than that derived from pH and acidity.To distinguish kimchi at 0 days and those stored at 4, 10, and 25°C, the organic acids content was used by performing linear discriminant analysis (LDA). As shown in Figure1, the differentiation index was found to be 100% for kimchi at 0 day and that stored at 10°C and 25°C, whereas it was 87.5% for the kimchi stored at 4°C. From the results of LDA given in Figure 1, the kimchi samples could be significantly distinguished using all its data of organic acids content at each storage temperature with a separation index of 95.16%.Figure 1
Distinction of kimchi samples at each storage temperature (0 days, 4°C, 10°C, and 25°C) by the organic acid content of each group using linear discriminant analysis (LDA).


## 3.1. Validation of Analytical Method

In validation of the HPLC analytical method, the values of LOD were in the range of 0.806–4.447 (mg/kg) and the LOQ values ranged from 2.442 to 13.475 (mg/kg) (Table2). The recovery (%) was found to be in the range of 90.12–109.81%. The CV% values obtained were all less than 3% (0.37–1.99%). Thus, the results obtained for the quality assurance parameters indicated that the applied analytical method is fully validated and fulfils the criteria required by AOAC.Table 2
Analytical parameters for HPLC analysis of organic acids.
Organic acid Correlation coefficient (R2) LOD1 (mg/kg) LOQ2 (mg/kg) Recovery3 (%) CV4 (%) Oxalic acid 0.99986 4.447 13.475 97.55 0.53 Citric acid 0.99978 1.136 3.442 91.60 0.54 Tartaric acid 0.99985 1.759 5.329 100.99 0.37 Malic acid 0.99972 1.683 5.101 107.21 1.29 Succinic acid 0.99955 1.321 4.003 109.81 1.26 Lactic acid 0.99958 1.503 4.555 93.35 0.81 Fumaric acid 0.99915 1.309 3.967 90.12 0.72 Acetic acid 0.99880 0.806 2.442 97.55 1.99 1 L i m i t of detection. 2Limit of quantification. 3Recovery of citric acid, malic acid, succinic acid, lactic acid, and acetic acid spiked at 1,000 ppm, oxalic acid spiked at 200 ppm, tartaric acid spiked at 500 ppm, and fumaric acid spiked at 5 ppm. 4Coefficient of variation.


## 3.2. Changes of pH during Storage

During the fermentation of kimchi, major components contained in Chinese cabbage and minor ingredients (salted seafood, chili powder, green onion, garlic, and ginger) are degraded or synthesized by microorganisms using various enzymes. The degradation of carbohydrates, a major component of Chinese cabbage, results in the formation of numerous organic acids which gives unique flavor to kimchi [5, 22, 23]. Thus, the pH, total acidity and organic acid of kimchi are important quality indices for determining the quality and storage level of kimchi. The pH changes in kimchi at 0 days and stored at 4 and 10°C for 8 weeks are summarized in Table 3. From the results, it can be noticed that the kimchi stored at 25°C for 2 days exhibited a pH of 4.1 and that at 0 days it exhibited a pH of 5.3. However, the one stored at 4°C showed a pH change ranged from 5.3 to 5.8 during 1–3 weeks which did not change considerably during early storage. However, as weeks passed, the pH gradually decreased to 4.1. The pH of kimchi stored at 10°C for 8 weeks exhibited an early rapid decrease from 5.8 at 0 days to 4.7 at 1 week. It gradually decreased until 6 weeks until it remained constant at 3.6–3.7. The pH values of the kimchi stored at 25°C were 5.3 at 0 day and 4.1±0.1 at 2 days. In general, the pH changes in kimchi at each temperature during fermentation exhibit a sigmoid curve: a slow decrease at the initial fermentation until it reaches 5.4 as fermentation progresses, rapid decrease to 4.2–4.4 at intermediate fermentation stage (the optimal fermentation stage), and a decrease to 4.0 at the final fermentation stage [8]. Such sigmoid curve is observed for the growth of living organisms which in case of kimchi is attributed to bacteria. In this study, the initial fermentation stage for kimchi stored at 4°C was observed until three weeks and the intermediate fermentation stage was observed over three weeks until the pH reached 4.1. However, kimchi stored at 10°C exhibited a very short initial fermentation stage and a rapid pH decrease in the intermediate fermentation stage. From 6 to 8 weeks when pH was 3.7, the final fermentation stage was observed with no changes in pH values. The kimchi produced in summer and stored at 4 and 10°C exhibited different trends in pH with storage over 8 weeks. The results suggest that low-temperature storage at 4°C allows a slower fermentation rate and permits long-term fermentation, whereas kimchi stored at 10°C exhibits high initial fermentation, which slows down once a certain fermentation level is reached. It further indicates that pH changes are more highly associated with temperature rather than salinity, implying that fermentation temperature largely affects the flavor of kimchi [24].Table 3
pH, acidity, and salinity of kimchi samples stored at 4 and 10°C1,2.
Storage period pH Acidity (%) Salinity (%) 0 days 5.84 ± 0.01 c 0.25 ± 0.00 p 2.03 ± 0.00 i  Storage period (weeks) and temperature (4°C) 1 5.76 ± 0.01 a 0.27 ± 0.01 q 1.84 ± 0.01 k 2 5.52 ± 0.01 b 0.36 ± 0.00 o 2.10 ± 0.01 h 3 5.31 ± 0.01 d 0.38 ± 0.01 n 2.28 ± 0.01 d 4 5.07 ± 0.00 e 0.42 ± 0.01 m 2.15 ± 0.01 g 5 4.90 ± 0.01 f 0.43 ± 0.01 l 2.23 ± 0.01 f 6 4.74 ± 0.00 g 0.53 ± 0.01 j 2.35 ± 0.01 b 7 4.27 ± 0.00 j 0.69 ± 0.02 h 2.52 ± 0.01 a 8 4.12 ± 0.00 k 0.79 ± 0.01 g 2.22 ± 0.02 f  Storage period (weeks) and temperature (10°C) 1 4.68 ± 0.01 h 0.51 ± 0.01 k 2.15 ± 0.02 g 2 4.33 ± 0.01 i 0.66 ± 0.01 i 2.14 ± 0.01 g 3 3.96 ± 0.01 l 1.01 ± 0.02 f 2.22 ± 0.01 f 4 3.85 ± 0.04 m 1.22 ± 0.02 e 2.32 ± 0.01 c 5 3.76 ± 0.01 n 1.26 ± 0.02 d 1.99 ± 0.00 j 6 3.70 ± 0.00 o 1.51 ± 0.02 b 1.81 ± 0.02 l 7 3.64 ± 0.00 q 1.48 ± 0.00 c 2.53 ± 0.01 a 8 3.68 ± 0.01 p 1.57 ± 0.01 a 2.25 ± 0.01 e  Storage period (days) and temperature (25°C) 2 4.1 ± 0.1 0.91 ± 0.1 2.22 ± 0.1 1Each value is mean ± SD (n=3). 2Means with the different superscript letters in the same column are significantly different (p<0.05).


## 3.3. Changes of Acidity during Storage

The results of acidity changes in kimchi, stored at 4 and 10°C for 8 weeks, are shown in Table3. The results showed that kimchi stored at 4°C showed no considerable acidity changes (0.3–0.4% from 0 day to 5 weeks). In contrast, the acidity of kimchi stored at 10°C increased from the early storage stage showing 0.3–1.57% increase from 0 days to 8 weeks. The acidity of kimchi stored at 10°C exhibited the initial, intermediate, and final stages in the same manner as that observed for pH changes, whereas that stored at 4°C showed a gradual increase in acidity. As a result, the acidity of kimchi stored at 10°C was 1.57% at 8 weeks, which was two times that of kimchi stored at 4°C (0.79%). The acidity of kimchi of 25 storage temperature exhibited the acidity of 0.25% and 0.9% at 0 and 2 days, respectively. During the fermentation of kimchi, various organic acids are generated by the reproduction of microorganisms which lower the pH and increase the acidity of kimchi [8]. An increase of total acidity and a decrease of pH have been reported to be significantly affected by microorganisms, sugar content, salt concentration, and temperature. Generally, the pH decline is a gradual decrease compared with the increase of total acidity, which is assumed to be caused by the buffer reaction of proteins and amino acids in kimchi [25]. The acidity does not increase significantly because aerobic microorganisms unassociated with acid production are temporarily active during the early ripening of kimchi, lactobacillus remains inactive and also because of water being released from Chinese cabbage [26]. In our study, the acidity of kimchi stored at 4°C gradually increased from 5 weeks, whereas kimchi stored at 10°C increased rapidly from 0 days to 5 weeks. These changes in acidity are significantly affected by ripening temperature and saline concentration as lactobacillus start to grow after their inactive period. From the previous studies, it was found that it took 2 days to reach full ripening at a salinity of 3% and a storage temperature of 20°C, 1 day at a salinity of 2.25%–3.50% and a storage temperature of 30°C, and 2–4 days at a salinity of 5% and a storage temperature of 30°C [27]. Considering that optimal fermentation occurs at an acidity and pH of 0.6% and 4.2, respectively, in the current study, kimchi ripened for 7 weeks at a storage temperature of 4°C exhibited a pH of 4.3 and acidity of 0.7%, whereas kimchi ripened for 2 weeks at a storage temperature of 10°C exhibited a pH of 4.3 and acidity of 0.7%. These optimal fermentation times were determined to be the time of full ripening above these conditions.


## 3.4. Changes of Salinity during Storage

In this study, the kimchi prepared at 0 day and that stored at 4 and 10°C were analyzed for the salinity differences. From the results given in Table3, the salt content ranged from 1.81% to 2.35%, suggesting that the salinity of kimchi did not change considerably at each storage time and temperature. The salinity of kimchi fermented at 25°C was found to be 2.03% and 2.22% at 0 and 2 days, respectively. From the previous literature, during the fermentation of kimchi with added abalone and kelp, the initial salinity was 1.37–1.67% and maintained at 2% because of the kimchi liquid and tissues of major and minor ingredients [28]. In another study, the kimchi made from summer Chinese cabbage of the alpine region, the salinity values of samples stored at 15°C and 5°C were 2.51–2.58 and 2.50–2.58, respectively, whereas measuring by the Mohr method has given slightly lower values of 2.41–2.48 [2]. Thus, several studies proved that salinity does not inhibit the growth of microorganisms for the fermentation of kimchi.


## 3.5. Changes in Organic Acids Content

In this study, a total of eight organic acids including oxalic acid, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, fumaric acid, and acetic acid were identified. The results of organic acids content of kimchi by various storage conditions are summarized in Table4. The results showed that the kimchi stored at 25°C for 2 days contained only oxalic acid, lactic acid, and acetic acid with the content levels (mg/kg) of 76.2±0.6, 4296.3±78.6, and 2913.3±6.9. As shown in Figure S1 (A) in Supplementary Material available online at https://doi.org/10.1155/2017/9562981, the kimchi stored at 4°C ripened for 8 weeks, malic acid exhibited the highest content during the early storage, and lactic acid exhibited the highest content at 8th week. The kimchi stored at 10°C ripened for 8 weeks, lactic acid content was markedly high at the early storage until 8 weeks followed by acetic acid content (Figure S1 (B)). Previous study reported that citric acid and malic acid gradually decrease as ripening progressed whereas lactic acid and acetic acid rapidly increase. Lactic acid was confirmed to be the main organic acid at the last stage of ripening [29]. Other study on kimchi added with abalone and kelp reported that citric acid, malic acid, and succinic acid contents were high during the initial fermentation stage and then gradually decreased as fermentation progressed, with the lactic acid and acetic acid contents increasing on days 7 and 14 of fermentation, respectively [28]. Furthermore, another study revealed that, after ripening of kimchi for 8 weeks at 5°C, the lactic acid which was low during the early storage exhibited a marked increase from day 13 of storage, whereas malic acid exhibited a decrease and the succinic acid, oxalic acid, and malonic acid contents did not change [30].Table 4
Organic acids content (mg/kg) in kimchi samples stored at 4 and 10°C1,2.
Storage period Oxalic acid Citric acid Tartaric acid Malic acid Succinic acid Lactic acid Fumaric acid Acetic acid 16.0 ± 0.5 1 , 362.3 ± 84.0 ND 1 , 546.6 ± 13.0 ND ND 11.5 ± 0.1 273.4 ± 9.8  Storage period (weeks) and temperature (4°C) 1 21.9 ± 0.8 e 1 , 565.0 ± 5.0 a ND 1 , 183.8 ± 14.6 b ND N D g 11.1 ± 0.1 a 279.7 ± 5.3 h 2 29.3 ± 0.7 c 1 , 250.6 ± 2.2 b ND 1 , 469.8 ± 32.6 a ND 188.1 ± 1.6 f 9.8 ± 0.1 b 391.4 ± 8.0 g 3 33.0 ± 1.1 b 1 , 220.9 ± 9.9 b c ND 906.5 ± 4.4 d ND 296.1 ± 6.9 e 9.6 ± 0.0 c 460.5 ± 9.4 e 4 29.0 ± 0.9 c 1 , 129.7 ± 10.6 d ND 1 , 046.8 ± 17.8 c ND 292.8 ± 4.9 e 8.0 ± 0.0 d 437.9 ± 9.4 f 5 25.7 ± 0.7 d 1 , 189.1 ± 44.2 c ND 770.8 ± 10.6 e ND 1 , 013.9 ± 67.1 d 7.9 ± 0.0 d 476.9 ± 12.0 d 6 36.6 ± 1.7 a 1 , 069.2 ± 11.5 e ND 305.0 ± 10.5 f ND 2 , 005.1 ± 12.4 c 4.9 ± 0.0 g 649.2 ± 2.9 c 7 29.1 ± 0.4 c 872.5 ± 35.4 f ND N D g ND 4 , 149.7 ± 24.2 b 6.5 ± 0.1 e 698.3 ± 2.1 b 8 31.5 ± 0.9 b 751.7 ± 1.9 g ND N D g ND 5 , 194.9 ± 32.5 a 5.7 ± 0.0 f 821.6 ± 8.7 a  Storage period (weeks) and temperature (10°C) 1 31.7 ± 1.4 c 576.6 ± 60.1 a ND 434.3 ± 15.6 a N D c 2 , 556.3 ± 32.7 h 6.4 ± 0.1 a 935.8 ± 14.6 f 2 24.9 ± 0.5 d 101.7 ± 0.9 b ND 229.6 ± 4.0 b N D c 3 , 676.4 ± 4.3 g 1.6 ± 0.0 b 1 , 378.3 ± 18.6 e 3 30.6 ± 0.7 c N D c ND N D c 365.3 ± 6.7 b 6 , 100.4 ± 45.8 f N D c 2 , 302.1 ± 51.2 d 4 34.8 ± 2.2 b N D c ND N D c 421.9 ± 18.7 a 7 , 459.9 ± 51.9 e N D c 2 , 710.6 ± 41.0 c 5 22.9 ± 0.7 d N D c ND N D c 343.3 ± 3.8 b 7 , 842.2 ± 161.3 d N D c 2 , 832.4 ± 81.5 b 6 35.2 ± 1.6 b N D c ND N D c 418.4 ± 19.4 a 11 , 922.4 ± 59.7 b N D c 2 , 978.1 ± 43.3 a 7 35.6 ± 1.6 b N D c ND N D c 343.4 ± 12.9 b 11 , 766.2 ± 41.3 c N D c 3 , 031.8 ± 25.3 a 8 38.3 ± 1.9 a N D c ND N D c 361.9 ± 4.6 b 12 , 621.2 ± 68.5 a N D c 2 , 800.9 ± 54.1 b  Storage period (days) and temperature (25°C) 2 76.2 ± 0.6 ND ND ND ND 4296.3 ± 48.6 ND 2913.3 ± 6.9 1Each value is mean ± SD (n=3). 2Means with the different superscript letters in the same column are significantly different (p<0.05).With regard to the present study, in case of kimchi stored at 4°C, the malic acid content was gradually decreased upon ripening and lactic acid content, which was not detected during early storage, rapidly increased at 5th week of storage. The kimchi stored at 10°C, lactic acid content was the highest from the early storage and its rate of increase became higher with increasing storage time. Malic acid content was not detected up to 2 weeks in the kimchi stored at 10°C and 25°C. Similarly, the malic acid content in kimchi stored at 4°C and 10°C rapidly decreased and was not detected at 6th and 4th week. The decrease in malic acid is reported to be caused by the conversion of malic acid to lactic acid and acetic acid by lactobacillus in kimchi [11]. The oxalic acid content did not change significantly with the storage period in kimchi stored at 4°C and 10°C, and it was two times higher in kimchi stored at 25°C. Fumaric acid had the lowest content (1.6–11.1 mg/kg).When changes of the organic acid contents were compared, it was found that the changes in acidity are mainly dependent on lactic acid content. The studies showed that kimchi exhibit a pH of 4.2–4.6 [8], acidity of 0.6–0.75% [16], and lactic acid content of 0.5–0.75% at the optimal fermentation time [31]. In the current study, kimchi stored at 4°C for 7 weeks and 10°C for 2 weeks was found to be the optimally fermented kimchi (Table 3). The lactic acid content in kimchi stored at 4°C for 7 weeks and that stored at 10°C for 2 weeks was 0.4% and 0.36%, respectively, lower than those reported by Kim (1985) [31]. The lactic acid content in kimchi stored at 4°C for 8 weeks and that stored at 10°C for 3 weeks was found to be 0.5% and 0.6%, respectively. Therefore, the optimal fermentation time for kimchi derived from lactic acid content appeared 1 week later than that derived from pH and acidity.To distinguish kimchi at 0 days and those stored at 4, 10, and 25°C, the organic acids content was used by performing linear discriminant analysis (LDA). As shown in Figure1, the differentiation index was found to be 100% for kimchi at 0 day and that stored at 10°C and 25°C, whereas it was 87.5% for the kimchi stored at 4°C. From the results of LDA given in Figure 1, the kimchi samples could be significantly distinguished using all its data of organic acids content at each storage temperature with a separation index of 95.16%.Figure 1
Distinction of kimchi samples at each storage temperature (0 days, 4°C, 10°C, and 25°C) by the organic acid content of each group using linear discriminant analysis (LDA).


## 4. Conclusions

The method of HPLC used for organic acids analysis was fully validated and the results were satisfied and in accordance with the criteria of Association of Official Analytical Chemists (AOAC) in all cases. The lactic acid content increased according to the storage and was found to be a major component to affect the pH and acidity of the kimchi. The results of the organic acids analysis, the kimchi stored for eight weeks at 4°C and that for three weeks at 10°C were similar. However, acetic acid detected was relatively high at 10°C which might lead to a different flavour of kimchi. The pH was found to be decreased with storage time whereas acidity was increased.



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*Source: 9562981-2017-01-15.xml*