# Strong Cation Exchange Chromatography in Analysis of Posttranslational Modifications: Innovations and Perspectives

Authors: Mariola J. Edelmann
Journal: Journal of Biomedicine and Biotechnology
 (2011)

---

## Abstract

Strong cation exchange (SCX) chromatography has been utilized as an excellent separation technique that can be combined with reversed-phase (RP) chromatography, which is frequently used in peptide mass spectrometry. Although SCX is valuable as the second component of such two-dimensional separation methods, its application goes far beyond efficient fractionation of complex peptide mixtures. Here I describe how SCX facilitates mapping of the protein posttranslational modifications (PTMs), specifically phosphorylation and N-terminal acetylation. The SCX chromatography has been mainly used for enrichment of these two PTMs, but it might also be beneficial for high-throughput analysis of other modifications that alter the net charge of a peptide.


---

## Body

## 1. Introduction

Posttranslational modifications (PTMs) regulate protein function, subcellular localization, and degradation. Although detection of the modified residues is crucial for understanding of the physiological roles of proteins as well as their mechanisms and pathways, it still represents a challenge. It is especially true in case of global proteome studies, in which hundreds or thousands of modified sites are monitored at the same time, and such analyses are becoming now more required due to an increasing popularity of the systems biology approaches. There is therefore a great demand for further development of efficient tools that could be used to characterize the entire complement of modified proteins in cells. Mass-spectrometry-based proteomics offers highly sensitive tools for modification mapping (reviewed in [1, 2]), where thousands of proteins can be concurrently analyzed to construct and validate comprehensive physiological models. Nevertheless, PTM analysis usually requires preceding enrichment methods due to the high complexity of the samples and the low abundance of modified peptides. In this paper I will discuss strong cation exchange (SCX) chromatography as an effective method for enrichment of PTMs, including phosphorylation and N-terminal acetylation (summarized in Table 1).Table 1
Overview of the SCX chromatography methods used for PTM analysis described in this paper.
Sample typeProtein amount/cell numberA number of fractions collectedMass spectrometer usedExperimental workflowOther detailsPTM analysisReferencesHeLa cells8 mg protein4 early fractions from each gel bandLCQ DECA XP ion trapSDS-PAGE (10 gel bands) → SCX → LC-MS/MS2002 phosphorylation sites identified from 967 proteins[6]HeLa cells0.5 mg protein10 fractionsLTQ-OrbitrapSCX → TiO2→ LC-MS/MS722 nonredundant phosphorylation sites[7]Human Chang liver cells1.5 mg protein40 fractionsQ-TOF and LCQSCX → TiO2→ LC-MS/MS1035 phosphorylation sites from 607 phosphoproteins[8]Escherichia coli cells20 mg protein10 fractionsLTQ-OrbitrapSCX → TiO2 → LC-MS/MSLys-C digestion followed by trypsin digestion81 phosphorylation sites from 79 proteins[9]Bacillus subtiliscells10 mg protein15 fractionsLTQ-Orbitrap or LTQ-FTSCX → TiO2→ LC-MS/MSLys-C digestion followed by trypsin digestion78 phosphorylation sites from 78 proteins[10]HeLa cells40 mg protein25 fractionsQSTAR ELITEIMAC → SCX → LC-MS/MS~4512 phosphorylated sites[11]HEK293 cells1 mg protein40 fractionsLTQ-OrbitrapSCX → LC-MS/MSLys-N, Lys-C, and trypsin digestion5036 nonredundant phosphorylation sites[4]Human embryonic stem cells10 mg protein12 fractionsLTQ-OrbitrapSCX → IMAC → LC-MS/MSCID and ETD fragmentation methods used in mass spectrometry10844 nonredundant phosphorylation sites[12]Membrane fraction of human teratocarcinoma Nt2/d1 cells5 × 106 cells~30 fractionsLTQ-OrbitrapSCX → LC-MS/MSLys-C digestion followed by trypsin digestion116 N-acetylated sites[13]HEK293 cells12.3 mg protein/6 × 107 cells49 fractionsLTQ XL Linear ion trapSCX → LC-MS/MSCID and ETD fragmentation methods used in mass spectrometry, Lys-N digestionN-acetylated peptides[14]Cytoplasmic, nuclear, and membrane fractions from Kc 167 cells (Drosophila melanogaster)n/an/aHCT ion trap, LTQ linear ion trap, and XCT-Ultra ion trapSCX → COFRADIC → LC-MS/MS861 N-acetylated sites[15]HEK293 cells1 mg protein~40 fractionsLTQ-OrbitrapSCX → LC-MS/MSLys-N, Lys-C, and trypsin digestion1391 N-acetylated peptides[16]SCX: strong cation exchange; PTMs: post-translational modifications; IMAC: immobilized metal affinity chromatography; ETD: electron transfer dissociation; CID: collision-induced dissociation; COFRADIC: combined fractional diagonal chromatography; LC-MS/MS: liquid chromatography-tandem mass spectrometry; n/a: data not available.


## 2. Principles of SCX Separation for Proteomic Applications

The SCX stationary phase usually contains aliphatic sulfonic acid groups that are negatively charged in aqueous solution, therefore tightly binding any strongly basic analytes. To recover the analyte, the resin is then washed with a solvent neutralizing this ionic interaction. Most tryptic peptides in acidic pH are characterized by a net charge of +2 and above, and they can be therefore separated by SCX from peptides possessing a net charge of +1, such as trypsin-generated phosphopeptides, C-terminal peptides, or peptides with blocked N-termini (i.e., peptides with blocked N-terminal free amine group, for instance, by N-acetylation), as well as from peptides containing higher charges, including ones containing missed cleavages and therefore more arginine and lysine residues [3]. Trypsin is not the only enzyme used to generate peptides for proteomic analyses, and other proteolytic enzymes are also utilized, for instance, Lys-N, Lys-C, Glu-C, or elastase. Some of these enzymes can be used to obtain peptides characterized by specific, distinguishable net charges, which provide additional advantages for SCX separation of differently modified peptides [4, 5].


## 3. SCX in Phosphoproteome Studies

The complex system of signal transduction enables effective and targeted cell responses to external or internal stimuli, and PTMs play here a major role. Phosphorylation is one of the best characterized among these PTMs. This modification has been shown to affect approximately 30% of all the human proteins [17], although recent studies suggest that this percentage is even higher [18]. Protein phosphorylation has been associated with a wide range of cellular processes, such as proliferation, migration, differentiation, cell cycle progression, and apoptosis [19, 20]. The mass spectrometric analysis of phosphopeptides can be very challenging for several reasons. Firstly, these negatively charged peptides are not easily analyzed in a positive mode, and this method of ionization is typically used in the electrospray ionization- (ESI-) based peptide mass spectrometry. Secondly, the hydrophilic property of phosphopeptides lowers their binding to the C18-based trapping columns used for purification of the peptide sample prior to its separation by HPLC. Thirdly, due to the ion suppression, detection of phosphopeptides by a mass spectrometer is problematic, especially in the complex samples containing unmodified or other more easily detectable peptides. Some of the above problems are still questionable, as discussed in [21], but the sole complexity of the peptide samples usually calls for prior enrichment in order to specifically select for phosphopeptides/phosphoproteins. These techniques include purification by phosphospecific antibodies [22, 23] and use of immobilized metal affinity chromatography (IMAC), where negatively charged phosphopeptides bind to positively charged metal ions, such as Fe3+, Ga3+, or Zr4+ [2, 24–26], as well as separation by titanium dioxide (TiO2) columns [27, 28]. Second chromatography dimension is also frequently utilized, and this might include hydrophilic interaction chromatography (HILIC) [29–32], electrostatic repulsion hydrophilic interaction chromatography (ERLIC) [11], and here discussed SCX.The SCX chromatography is the most widely used HPLC fractionation method, and it has also found applications in phosphoproteomics (Table1). The peptides are eluted from an SCX column using a low-pH buffer, and in these conditions phosphopeptides have a net charge of 0 or +1, as opposed to a majority of other tryptic peptides characterized by +2 or higher net charges. Therefore, most of the phosphopeptides elute in the first few SCX fractions, although this depends on the type of gradient used and on a number of fractions collected [3, 33–35].Because of these features, the SCX chromatography can be employed as a stand-alone method for phosphopeptide mapping, as demonstrated by Beausoleil et al. [6], who used it without additional IMAC or TiO2 phosphopeptide enrichment. In this experiment 8 mg of the nuclear protein extract derived from HeLa cells was first separated by SDS-PAGE, then ten gel bands were digested by trypsin and the peptides were resolved by SCX, where only the first four fractions were collected for subsequent LC-MS/MS analysis by LCQ DECA XP ion trap. This simple workflow allowed for identification of 2002 phosphorylation sites from 967 proteins. Adding another dimension to this separation, such as an off-gel fractionation, which is based on the isoelectric point of peptides/proteins, might result in even larger number of identified phosphopeptides without enrichment by IMAC, TiO2, or phosphospecific antibodies.Due to the complexity of the protein samples, SCX-based phosphopeptide separation alone is often ineffective, and the secondary phosphopeptide enrichment is necessary for identification of a larger number of phosphorylated residues [33, 36, 37]. For instance, an automated SCX-TiO2-RP-LC-MS/MS method was used to separate HeLa protein digest, where 722 nonredundant phosphorylation sites were identified, including several doubly and triply phosphorylated peptides [7]. Briefly, 500 μg of HeLa protein extract was digested, and these peptide mixtures were separated either by SCX or strong anion exchange (SAX) columns. In case of SCX, each of ten SCX fractions was additionally subjected to the phosphopeptide enrichment by TiO2. The samples were analyzed by the LTQ-Orbitrap mass spectrometer combined with C18-based HPLC. This SCX method was complementary to the on-line SAX separation, which however did not use subsequent TiO2 enrichment. In comparison with SCX, the latter method was able to separate a higher number of multiply phosphorylated peptides [7], but this could be due to the TiO2 enrichment used after the SCX chromatography which is known to be less ideal for multiply phosphorylated peptide analysis. Furthermore, Sui et al. used similar SCX separation followed by TiO2 enrichment and LC-MS/MS analysis to map the phosphoproteome in human Chang liver cell line [8]. In this experiment, 1.5 mg protein was digested with trypsin and subjected to separation by SCX chromatography into forty fractions, followed by enrichment by TiO2 and LC-MS/MS analysis by Q-TOF and LCQ mass spectrometers. This study identified 1035 phosphorylation sites on 607 phosphoproteins, including a large fraction of previously unknown phosphoproteins, and the functional analysis demonstrated that majority of these proteins were involved in liver metabolism. Finally, similar procedure has been used for the phosphoproteome analysis of a Gram-negative bacterium Escherichia coli,where 81 phosphorylation sites were identified on 79 proteins [9]. In this study, 20 mg protein was digested with Lys-C followed by tryptic digestion. The resulted peptides were split into two technical replicates and separated on SCX column into ten fractions, followed by TiO2 phosphopeptide purification and analysis by HPLC coupled to LTQ-Orbitrap mass spectrometer. A comparison of this dataset to a phosphoproteomic study of bacterium Bacillus subtilis [10] indicated that both phosphoproteomes demonstrate high conservation levels, and the authors suggested that some phosphorylation sites are conserved from Archaea to humans [9]. The Bacillus subtilis study has been done using a very similar technique to the one described for E. coli[9], but 10 mg protein was used for analysis and fifteen SCX fractions were collected, which were then subjected to the TiO2 chromatography and analysis by HPLC coupled to LTQ-Orbitrap or LTQ-FT mass spectrometry.Although SCX is a relatively old separation method, it still performs quite well if compared with newer HPLC techniques. Chen et al. [11] evaluated four different chromatography methods in terms of their capabilities to separate phosphopeptides of HeLa cells, and these included SCX, HILIC, ERLIC chromatography with nonvolatile solvent system (ERLIC-nV), and ERLIC with volatile solvent system (ERLIC-V). Each chromatography method was performed on peptides obtained from 40 mg protein. Phosphopeptides were first enriched on IMAC, followed by SCX, HILIC, ERLIC-nV, or ERLIC-V-based separation into multiple fractions (25 fractions in case of SCX) and finally analyzed by LC-MS/MS using QSTAR ELITE mass spectrometer. As a result, each technique yielded between 4000 and 5000 phosphopeptides, including a large number of nonoverlapping phosphopeptides for each HPLC method. Together, the data from all four chromatography analyses generated 9069 unique phosphopeptides, demonstrating a complementary nature of these different chromatography methods. Interestingly, the phosphopeptide resolving power was the greatest for the SCX method (60.52% nonrepetitive phosphopeptides), followed by ERLIC-nV (50.79% nonrepetitive phosphopeptides), while the lowest performance was assigned to HILIC (50.83% nonrepetitive phosphopeptides), which also had the worst orthogonality relative to reversed-phase chromatography.Apart from combining SCX separation with additional phosphopeptide enrichment methods, another innovation is to utilize other proteolytic enzymes for peptide generation in order to complement the data obtained from trypsin-digested samples. An example is Lys-N [4, 5] that generates phosphopeptides characterized by distinct net charges, which can be thus efficiently separated by SCX and distinguished from the N-acetylated peptide populations [4]. This feature of Lys-N has been taken advantage of in a study where a parallel use of Lys-N, trypsin, and Lys-C digestion followed by SCX and LC-MS/MS analysis provided a total of 5036 nonredundant phosphopeptides [4]. In this study only 1 mg protein was subjected to digestion with one of these proteolytic enzymes. The peptides were then desalted on C18 columns and subjected to the SCX-based fractionation. Forty thus obtained SCX fractions were analyzed by HPLC combined with LTQ-Orbitrap mass spectrometry. Lys-N increased the phosphopeptide coverage by 72% if compared with the trypsin-digested sample, whereas trypsin accounted for only 25% of the increase. Clearly, future exploration of other proteolytic enzymes can additionally benefit the global phosphoproteome studies.In addition, the mass spectrometry methods can also be modified to fully exploit the phosphopeptide separation capabilities provided by SCX. As an example, electron transfer dissociation (ETD) has emerged as an alternative to collision-induced dissociation (CID) fragmentation method used in mass spectrometry. ETD is especially suitable for analysis of highly charged peptides and for PTMs that are labile upon CID fragmentation methods [38]. As an example, this approach has been used to characterize phosphoproteome of human embryonic stem cells [12]. In this study, peptides obtained from 10 mg protein lysate were subjected to the SCX separation into twelve fractions, followed by IMAC phosphopeptide purification and LC-MS/MS analysis by the LTQ-Orbitrap mass spectrometer. The peptides were analyzed using ETD and CID fragmentation methods, which resulted in the identification of 10,844 nonredundant phosphorylation sites. Importantly, the ETD method yielded a much larger number of unique phosphopeptides than CID (8,087 in comparison with 3,868). In this study, ETD fragmentation analysis has been also shown to more frequently localize the phosphorylation site to a specific residue, which is often problematic when using CID fragmentation [12].Finally, my preliminary data suggests that SCX chromatography resins can be further optimized in terms of their phosphopeptide enrichment properties. I used TiO2 resin as well as cartridges containing three different SCX resins to separate phosphorylated peptides of several forms of casein, followed by LS-MS/MS analysis (please, refer to the Supplementary methods in Appendix). The SCX resins included sulfonic acid (SCX-1), propyl sulfonic acid (SCX-2), and ethylbenzene sulfonic acid (SCX-3), and their performance varied in terms of their phosphopeptide enrichment potential. In this experiment, SCX-3 has been shown to be the most efficient column among the tested resins (Figure 1). Due to the presence of an aromatic ring, SCX-1 and SCX-3 resins have nonpolar character, therefore enhancing nonpolar secondary interactions with the analyte and increasing its binding to the column. However, SCX-3 might have also other characteristics that enable for a slightly better separation of phosphopeptides. This preliminary study should be repeated using a more complex sample, but it demonstrates that there might be a bigger potential for the SCX-based separation methods if these resins are further optimized specifically for phosphorylation analysis.Figure 1
Phosphopeptide enrichment capabilities for various SCX resins. 50 μg casein was digested with trypsin and loaded in triplicates onto SCX-1, SCX-2, SCX-3 cartridges, and TiO2-packed tips, followed by peptide elution. In case of the SCX cartridges, the sequential peptide elution was done using four buffers (A–D) characterized by increasing molarity of ammonium acetate (50, 100, 200, or 500 nM ammonium acetate). The TiO2 tips were preconditioned with 100% acetonitrile, conditioned with 0.2 M phosphate buffer pH7, and equilibrated with solvent containing 50% acetonitrile and 0.1% formic acid. The peptide sample was then loaded onto TiO2 resin, washed six times with buffer containing 50% acetonitrile, 0.1% formic acid, and 0.1 M KCl, and eluted with 0.2 M phosphate buffer pH 7 and 0.5% aqueous ammonia; both TiO2 eluates were combined. The volume of each eluate was concentrated, and triplicate samples were analyzed by LC-MS/MS (Esquire HCTplus mass spectrometer, Bruker Daltonics). The displayed percentage of phosphorylated peptides was calculated for three forms of casein (UniProt accession numbers P02663, P02662, and P02663). Standard deviation was calculated using experimental triplicates.


## 4. Utilizing SCX Chromatography in Analysis of N-Terminal Acetylation

The N-terminal acetylation is a common protein modification with a relatively unclear physiological role. It affects a variety of protein functions, such as enzymatic activity, DNA-binding, and protein-protein interactions [39]. It might also represent a specific degron site, thus targeting proteins for ubiquitination and subsequent degradation [40]. The SCX-based separation of N-terminal acetylated peptides is based on the fact that these peptides lack the N-terminal free amine, which contributes to their earlier elution in the SCX chromatography [13] and enables their separation from slightly later migrating phosphopeptides (Table 1; [4, 14]).One of the first attempts to use SCX for analysis of the N-acetylated and C-terminal peptides showed that this approach, if adequately optimized, can provide 95% specificity for this peptide class [13]. The membrane-enriched fraction from 5 × 106 human teratocarcinoma Nt2/d1 cells was digested with Lys-C, followed by tryptic digestion. The peptides were desalted with a C18 column and separated by SCX into approximately thirty fractions, which were then analyzed by the LC-LTQ-Orbitrap mass spectrometry. This workflow proved to be successful, and it allowed for identification of 116 N-terminal acetylated residues, although a relatively small amount of protein material was used.Moreover, similarly as in case of phosphoproteome analysis, the SCX-based separation of N-terminal acetylated peptides has been combined with the ETD fragmentation method [14], yielding results complementary to the ones obtained by the CID fragmentation. Digestion with proteolytic enzymes such as Lys-N and Lys-C has also been shown to be advantageous, for instance, due to already mentioned separation of the N-terminally acetylated peptides from phosphopeptides [4, 14, 16].Furthermore, there are additional methods suitable for enrichment of the N-terminal acetylated peptides that can be used in conjunction with SCX, and these include combined fractional diagonal chromatography (COFRADIC; summarized in [41]). For instance, SCX combined with COFRADIC and LC-MS/MS analysis by an ion trap mass spectrometer has been used to identify over 1,200 mature protein N-termini, including 861 N-terminal acetylation sites in cytoplasmic, nuclear, and membrane fractions obtained from Drosophila melanogastercells [15].In summary, the SCX methods used for mapping of the N-terminal acetylation sites are expected to shed more light on the abundance and function of this prevalent but not well-understood modification.


## 5. Conclusions

Although current efforts are focused on optimizing the SCX chromatography for analysis of phosphorylation as a key signaling modification, this HPLC method has a potential to be used in analysis of other PTMs that alter a net charge of the peptides. For example, SCX could be possibly used in glycomics, based on the assumption that many extracellular N-glycans contain sialic acid residues, thereby reducing the net charge of glycopeptides in comparison with the unmodified peptides [42]. Moreover, modifications of lysine and arginine residues, such as methylation or acetylation, also account for a shift in SCX elution time in comparison with their unmodified counterparts [43]. When compared to the whole complement of peptides, these modifications might not cause a sufficient change in elution properties in order to monitor these modifications on a global scale, but they can still be optimized to study modifications of specific proteins, such as histones that are characterized by multiple lysine and arginine PTMs. Moreover, differential elution of peptides containing modified arginine or lysine residues in comparison with peptides containing these amino acids in an unmodified form can be used as an additional validation of the proteomic results. All this further underlines the importance of the SCX chromatography in proteomic applications focused on investigation of protein modifications.



---

*Source: 936508-2011-11-17.xml*