# Influence of Soybean (Glycine max) Population and Herbicide Program on Palmer Amaranth (Amaranthus palmeri) Control, Soybean Yield, and Economic Return

Authors: Amy E. Hoffner; David L. Jordan; Alan C. York; E. James Dunphy; Wesley J. Everman
Journal: ISRN Agronomy
 (2012)

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## Abstract

Palmer amaranth (Amaranthus palmeri S. Wats) has become one of the most prominent and difficult weeds to control in soybean (Glycine max (L.) Merr.) in North Carolina. A survey was conducted in North Carolina during fall 2010 to estimate the magnitude of this problem. Palmer amaranth was present in 39% of 2,512 fields representing 0.24% of soybean ha in North Carolina. In recent years, growers have reduced soybean seeding rates in an effort to decrease production costs associated with technology fees. However, given the increase in prevalence of Palmer amaranth and the difficultly in controlling this weed due to herbicide resistance, growers may need to reconsider reductions in seeding rates. Therefore, research was conducted during 2010 and 2011 to determine if Palmer amaranth control, soybean yield, and economic return were affected by soybean plant population, preemergence (PRE) and postemergence (POST) herbicides, and herbicide resistant traits (glufosinate-resistant and glyphosate-resistant cultivars). Applying PRE or POST herbicides and increasing soybean population increased Palmer amaranth control, soybean yield, and economic return when compared with POST herbicides only or when lower soybean populations were present. Efficacy of glufosinate and glyphosate did not vary in most instances, most likely because these herbicides were applied timely, and the frequency of glyphosate resistance did not exceed 10% in these fields.


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## Body

## 1. Introduction

Effective weed management continues to be an important component of profitable soybean production in North Carolina. In recent years, herbicide-resistant weed populations have developed and have limited the effectiveness of herbicide options for growers. Populations of Palmer amaranth and horseweed (Conyza canadensis L.) in the coastal plain of North Carolina resistant to glyphosate have been confirmed [1, 2]. Resistance of Palmer amaranth to acetolactate synthase (ALS)-inhibiting herbicides also has been confirmed in North Carolina [1, 2]. Managing herbicide-resistant weed populations continues to be a challenge, especially Palmer amaranth, where alternatives to glyphosate and ALS-inhibiting herbicides require timely application [3, 4]. During fall 2010, 242 specific sites were sampled in North Carolina to determine the geographical range of Palmer amaranth and to determine if these populations were resistant to glyphosate. Of these specific sites, 52% had Palmer amaranth present with 98% of these populations expressing resistance to glyphosate and 85% expressing resistance to thifensulfuron-methyl [5]. Glyphosate-resistant Palmer amaranth has become the most challenging weed in cotton (Gossypium hirsutum L.) and soybean production in the southeastern United States [6–8].Cultural practices, such as cultivar selection, row pattern, and plant populations, can influence weed management [9, 10]. Residual herbicides applied PRE and POST herbicides other than glyphosate are important in managing glyphosate-resistant Palmer amaranth in soybean [11–13]. Glufosinate applied to glufosinate-resistant cultivars is an alternative to glyphosate for control of Palmer amaranth. Developing a strategy for controlling Palmer amaranth that is as sustainable and effective as programs in glyphosate-resistant crops has become one of the most important weed management challenges in the southeastern United States [11–13].In North Carolina, soybean at populations below those established in many fields yield as well as higher populations considered “standard” (exceeding 300,000 plants ha−1) by the farming community (Dunphy, E. J., personal communication). Reducing input costs in soybean by using lower seeding rates, especially when transgenic cultivars are planted which include substantial technology fees, is a reasonable approach to soybean production. While research has demonstrated that higher plant populations and narrow rows suppress weeds more effectively than lower plant populations or production in wider row spacings [14–17], Norsworthy and Oliver [18] reported that applying a second application of glyphosate was more economical than increasing the seeding rate. Kratochvil et al. [19] reported that a population 80% of a traditional seeding rate (345,000 plants ha−1 versus 432,500 seed ha−1) increased profit from $14 to $28 ha−1. However, under high populations of weeds, and most especially glyphosate-resistant Palmer amaranth, there is concern that lower populations will be susceptible to greater weed interference and possibly lower yield. Owen [12] indicated that increased difficulty in controlling weeds with glyphosate most likely will occur due to prolonged use of glyphosate. Guillermo et al. [14] suggested that higher seeding rates should be used in fields where weeds are difficult to control due to shifts in populations or development of herbicide resistance within weed communities. Given that Palmer amaranth resistance to glyphosate has increased dramatically in North Carolina [2, 5], research is needed to better define the role of seeding rate on weed management programs for soybean. Therefore, research was conducted at five locations in North Carolina during 2010 and 2011 to compare Palmer amaranth control, soybean yield, and economic return with combinations of PRE and POST herbicides applied to glufosinate- and glyphosate-resistant cultivars at two soybean populations. A survey of fields to determine the extent of Palmer amaranth infestation also was conducted during 2010 within the latter portion of the growing season.


## 2. Materials and Methods

### 2.1. Survey of Soybean Fields in North Carolina

A total of 2,512 soybean fields in North Carolina were surveyed during 2010 from mid August through September to determine the extent of Palmer amaranth escapes. The area represented in these fields was 0.24% of the 62,700 ha of soybean harvested in North Carolina during 2010 [20]. The procedure involved estimating the percentage of total ha within each field with at least one Palmer amaranth plant present. The survey did not involve walking or driving across soybean fields but was a result of viewing the field from one side. Although the average field size in the survey was 6.2 ha, this procedure most likely under estimated infestation in some fields. The percentage of ha with Palmer amaranth was grouped into fields with up to 50% of ha infested and fields with more than 50% of ha infested.


### 2.2. Interactions of Plant Population, Herbicide Resistant Trait, and Herbicide Program

The experiment was conducted in five fields in North Carolina during 2010 and 2011 at the Upper Coastal Plain Research Station near Rocky Mount in fields with natural and relatively high populations of Palmer amaranth. Although other weed species were present, populations were low and inconsistent across fields compared with density and distribution of Palmer amaranth. The experiment was conducted during both years in one field (referred to as Field B13) consisting of a Lynchburg fine sandy loam soil (fine-loamy, siliceous, semiactive, thermic Aeric Paleaquults). The experiment was also conducted in Field E2 (2010) and Field E3 (2011) consisting of the same soil series. A final experiment in 2011 was conducted in Field C11 consisting of a Nahunta loam (fine-silty, siliceous, subactive, thermic Aeric Paleaquults). Soybean was planted after disking and field cultivation in rows spaced 20 cm apart. Plot size was 12 rows by 9 m in length. In 2010, soil moisture conditions were marginal for soybean emergence at planting and resulted in lower than desired populations but adequate for production. Soil conditions at planting during 2011 were very dry and fields were irrigated with a traveling gun system delivering 1.5 cm within 3 d after planting to ensure uniform emergence.Treatments consisted of two levels of herbicide resistance trait (cultivars expressing either glyphosate- or glufosinate-resistance) (HRT), two levels of soybean population (referred to as low or high), three levels of PRE herbicide (none,S-metolachlor, S-metolachlor plus fomesafen), and two levels of POST herbicide (none and a single POST application). The glufosinate-resistant cultivar was LL 595 N (Southern States Cooperative, Inc., 9120 W. Marlboro St., Farmville, NC 27828). The glyphosate-resistant cultivar was AG5605 (Monsanto Company, St. Louis, MO 63107). S-metolachlor (Dual II Magnum herbicide, Syngenta Crop Protection, Greensboro, NC 27709) and S-metolachlor plus fomesafen (Prefix herbicide, Syngenta Crop Protection, Greensboro, NC 27709) were applied at 1500 and 1200 + 270 g ae ha−1, respectively. Glufosinate-ammonium (Ignite 280 herbicide, Bayer CropScience, Research Triangle Park, NC 27709) and potassium salt of glyphosate (Roundup PowerMAX  herbicide Monsanto Company, St. Louis, MO 63107) were applied at 560 g and 840 g ae ha−1, respectively. Herbicides were applied using a CO2-pressurized backpack sprayer calibrated to deliver 145 L ha−1 using 8002 regular flat-fan nozzles (Teejet Corporation, Wheaton, IL 60187) at 275 kPa. Planting dates, dates of herbicide application, and soybean populations are listed in Table 1.Table 1
Planting date, soybean plant population, dates of preemergence and postemergence herbicide application, and estimated level of resistance to glyphosate at Rocky Mount during 2010 and 2011.
Soybean population
Date of herbicide application
Year
Field
Planting date
Low
High
Preemergence
Postemergence
Resistance to glyphosatea
Plants ha−1
2010
B13
May 24
122,000
342,000
May 24
June 10
Yes (5%)
2010
E2
May 24
145,000
464,000
May 24
June 10
No
2011
B13
June 7
221,000
560,000
June 7
June 21
Yes (5%)
2011
C11
June 7
208,000
519,000
June 7
June 21
Yes (10%)
2011
E3
June 7
193,000
533,000
June 7
June 21
No
aResistance to glyphosate was not confirmed in these fields but has been confirmed previously at this location during 2008; percentages refer to estimated percent of Palmer amaranth population surviving glyphosate application.Soybean and Palmer amaranth densities were determined 2 weeks after planting immediately prior to POST herbicide application by counting plants in two, 0.5-m randomly selected areas within each plot (Tables1 and 2). Visible estimates of percent Palmer amaranth control were recorded 2 and 8 weeks after POST herbicides were applied using a scale of 0 to 100, where 0 = no control and 100 = complete control. Soybean yield was determined, and estimated economic return was calculated based on North Carolina Cooperative Extension Service enterprise budgets [21]. Seed, overhead, management, and weed control costs were removed from the budget to establish a base production cost of $393 ha−1. Appropriate seed, herbicide, and application costs were then added based on treatments in the experiment. Seed cost was determined based on $99 ha−1 for a seeding rate of 345,800 plants ha−1 for both cultivars. Cost of S-metolachlor, S-metolachlor plus fomesafen, glufosinate, and glyphosate were set at $41 ha−1, $42 ha−1, $45 ha−1, and $15 ha−1, respectively. Application cost was $9.88 ha−1. Soybean price was set at $0.44 kg−1.Table 2
Palmer amaranth population as affected by preemergence herbicides at Rocky Mount, NC, during 2010 and 2011.a
Year
Field
Palmer amaranth population
No preemergence herbicide
S-metolachlor
S-metolachlor plus fomesafen
Plants m−2
2010
B13
124a
4b
1b
2010
E2
165a
10b
6b
2011
B13
74a
5b
0b
2011
C11
163a
4b
0b
2011
E3
76a
5b
0b
aMeans within a year and field combination followed by the same letter are not significantly different based on Fisher’s Protected LSD test at P≤ 0.05.  Data are pooled over soybean populations and herbicide resistance traits.  Postemergence herbicides have not been applied when Palmer amaranth density was recorded.The experimental design was a split-plot with HRT serving as whole plot units and combinations of soybean populations and herbicide treatments serving as sub-plot units. Treatments were replicated four times. Data for Palmer amaranth control, soybean yield, and estimated economic return were subjected to ANOVA using the Proc GLM procedure in SAS (SAS Institute, GLM Procedure, 100 Campus Drive, Cary, NC 27513) by experiment (combination of field and year) for a two (HRT) by two (soybean population) by three (PRE herbicide treatment) by two (POST herbicide treatment) factorial arrangement of treatments. Palmer amaranth control 2 weeks after POST herbicide application generally reflected control 8 weeks after POST herbicide application and will be the only visible ratings discussed. Significant year and field interactions were noted for Palmer amaranth control, soybean yield, and estimated economic return. Therefore, data are presented individually by year/field combination. Means of significant main effects and interactions were separated using Fisher’s Protected LSD test atP≤0.05. Main effects and interactions not stated in the text exceeded P=0.05.


## 2.1. Survey of Soybean Fields in North Carolina

A total of 2,512 soybean fields in North Carolina were surveyed during 2010 from mid August through September to determine the extent of Palmer amaranth escapes. The area represented in these fields was 0.24% of the 62,700 ha of soybean harvested in North Carolina during 2010 [20]. The procedure involved estimating the percentage of total ha within each field with at least one Palmer amaranth plant present. The survey did not involve walking or driving across soybean fields but was a result of viewing the field from one side. Although the average field size in the survey was 6.2 ha, this procedure most likely under estimated infestation in some fields. The percentage of ha with Palmer amaranth was grouped into fields with up to 50% of ha infested and fields with more than 50% of ha infested.


## 2.2. Interactions of Plant Population, Herbicide Resistant Trait, and Herbicide Program

The experiment was conducted in five fields in North Carolina during 2010 and 2011 at the Upper Coastal Plain Research Station near Rocky Mount in fields with natural and relatively high populations of Palmer amaranth. Although other weed species were present, populations were low and inconsistent across fields compared with density and distribution of Palmer amaranth. The experiment was conducted during both years in one field (referred to as Field B13) consisting of a Lynchburg fine sandy loam soil (fine-loamy, siliceous, semiactive, thermic Aeric Paleaquults). The experiment was also conducted in Field E2 (2010) and Field E3 (2011) consisting of the same soil series. A final experiment in 2011 was conducted in Field C11 consisting of a Nahunta loam (fine-silty, siliceous, subactive, thermic Aeric Paleaquults). Soybean was planted after disking and field cultivation in rows spaced 20 cm apart. Plot size was 12 rows by 9 m in length. In 2010, soil moisture conditions were marginal for soybean emergence at planting and resulted in lower than desired populations but adequate for production. Soil conditions at planting during 2011 were very dry and fields were irrigated with a traveling gun system delivering 1.5 cm within 3 d after planting to ensure uniform emergence.Treatments consisted of two levels of herbicide resistance trait (cultivars expressing either glyphosate- or glufosinate-resistance) (HRT), two levels of soybean population (referred to as low or high), three levels of PRE herbicide (none,S-metolachlor, S-metolachlor plus fomesafen), and two levels of POST herbicide (none and a single POST application). The glufosinate-resistant cultivar was LL 595 N (Southern States Cooperative, Inc., 9120 W. Marlboro St., Farmville, NC 27828). The glyphosate-resistant cultivar was AG5605 (Monsanto Company, St. Louis, MO 63107). S-metolachlor (Dual II Magnum herbicide, Syngenta Crop Protection, Greensboro, NC 27709) and S-metolachlor plus fomesafen (Prefix herbicide, Syngenta Crop Protection, Greensboro, NC 27709) were applied at 1500 and 1200 + 270 g ae ha−1, respectively. Glufosinate-ammonium (Ignite 280 herbicide, Bayer CropScience, Research Triangle Park, NC 27709) and potassium salt of glyphosate (Roundup PowerMAX  herbicide Monsanto Company, St. Louis, MO 63107) were applied at 560 g and 840 g ae ha−1, respectively. Herbicides were applied using a CO2-pressurized backpack sprayer calibrated to deliver 145 L ha−1 using 8002 regular flat-fan nozzles (Teejet Corporation, Wheaton, IL 60187) at 275 kPa. Planting dates, dates of herbicide application, and soybean populations are listed in Table 1.Table 1
Planting date, soybean plant population, dates of preemergence and postemergence herbicide application, and estimated level of resistance to glyphosate at Rocky Mount during 2010 and 2011.
Soybean population
Date of herbicide application
Year
Field
Planting date
Low
High
Preemergence
Postemergence
Resistance to glyphosatea
Plants ha−1
2010
B13
May 24
122,000
342,000
May 24
June 10
Yes (5%)
2010
E2
May 24
145,000
464,000
May 24
June 10
No
2011
B13
June 7
221,000
560,000
June 7
June 21
Yes (5%)
2011
C11
June 7
208,000
519,000
June 7
June 21
Yes (10%)
2011
E3
June 7
193,000
533,000
June 7
June 21
No
aResistance to glyphosate was not confirmed in these fields but has been confirmed previously at this location during 2008; percentages refer to estimated percent of Palmer amaranth population surviving glyphosate application.Soybean and Palmer amaranth densities were determined 2 weeks after planting immediately prior to POST herbicide application by counting plants in two, 0.5-m randomly selected areas within each plot (Tables1 and 2). Visible estimates of percent Palmer amaranth control were recorded 2 and 8 weeks after POST herbicides were applied using a scale of 0 to 100, where 0 = no control and 100 = complete control. Soybean yield was determined, and estimated economic return was calculated based on North Carolina Cooperative Extension Service enterprise budgets [21]. Seed, overhead, management, and weed control costs were removed from the budget to establish a base production cost of $393 ha−1. Appropriate seed, herbicide, and application costs were then added based on treatments in the experiment. Seed cost was determined based on $99 ha−1 for a seeding rate of 345,800 plants ha−1 for both cultivars. Cost of S-metolachlor, S-metolachlor plus fomesafen, glufosinate, and glyphosate were set at $41 ha−1, $42 ha−1, $45 ha−1, and $15 ha−1, respectively. Application cost was $9.88 ha−1. Soybean price was set at $0.44 kg−1.Table 2
Palmer amaranth population as affected by preemergence herbicides at Rocky Mount, NC, during 2010 and 2011.a
Year
Field
Palmer amaranth population
No preemergence herbicide
S-metolachlor
S-metolachlor plus fomesafen
Plants m−2
2010
B13
124a
4b
1b
2010
E2
165a
10b
6b
2011
B13
74a
5b
0b
2011
C11
163a
4b
0b
2011
E3
76a
5b
0b
aMeans within a year and field combination followed by the same letter are not significantly different based on Fisher’s Protected LSD test at P≤ 0.05.  Data are pooled over soybean populations and herbicide resistance traits.  Postemergence herbicides have not been applied when Palmer amaranth density was recorded.The experimental design was a split-plot with HRT serving as whole plot units and combinations of soybean populations and herbicide treatments serving as sub-plot units. Treatments were replicated four times. Data for Palmer amaranth control, soybean yield, and estimated economic return were subjected to ANOVA using the Proc GLM procedure in SAS (SAS Institute, GLM Procedure, 100 Campus Drive, Cary, NC 27513) by experiment (combination of field and year) for a two (HRT) by two (soybean population) by three (PRE herbicide treatment) by two (POST herbicide treatment) factorial arrangement of treatments. Palmer amaranth control 2 weeks after POST herbicide application generally reflected control 8 weeks after POST herbicide application and will be the only visible ratings discussed. Significant year and field interactions were noted for Palmer amaranth control, soybean yield, and estimated economic return. Therefore, data are presented individually by year/field combination. Means of significant main effects and interactions were separated using Fisher’s Protected LSD test atP≤0.05. Main effects and interactions not stated in the text exceeded P=0.05.


## 3. Results and Discussion

### 3.1. Survey of Soybean Fields in North Carolina

The percentage of ha within the 2,512 fields with at least one Palmer amaranth plant in the field was estimated to be 39% with 61% not having Palmer amaranth present (data not shown in tables). The percentage of fields with between 1 and 50% of ha infested was 22% while 17% of fields were infested at a level of 51 to 100% by Palmer amaranth (data not presented in tables). Results from this survey, combined with those of Hoffner et al. [5], reveal the magnitude of Palmer amaranth infestation in North Carolina.


### 3.2. Interactions of Plant Population, Herbicide Resistant Trait, and Herbicide Program

Palmer amaranth population in absence of PRE herbicides ranged from 74 to 165 plants m−2 (Table 2). When S-metolachlor or S-metolachlor plus fomesafen was applied, the Palmer amaranth population decreased to 10 plants m−2 or less. No difference in Palmer amaranth density was noted when comparing S-metolachlor and S-metolachlor plus fomesafen. When activated by rainfall or irrigation, these herbicides often control Palmer amaranth for several weeks during the season [22].The interaction of soybean population by POST herbicide was significant for Palmer amaranth control in Field E2 during 2010. When pooled over HRT and PRE herbicides, control was 50 to 55% without POST herbicides (data not shown in tables). Applying a POST herbicide to the low seeding rate increased control to 81%, while the combination of the high seeding rate and POST herbicide controlled Palmer amaranth 94% (data not shown in tables). Soybean population also influenced the interaction of PRE herbicide and HRT in this field. In absence of PRE herbicides, Palmer amaranth was controlled more effectively when glufosinate was applied in the high population compared with the low population (Table3). The most effective control in this HRT was S-metolachlor plus fomesafen in the high population. Although PRE herbicides increased control when glyphosate was applied, control was not affected by soybean population (Table 3). In contrast to these results, soybean population did not interact with HRT or herbicide treatments in Fields B13 and C11. When pooled over HRT and PRE and POST herbicides, Palmer amaranth control was greater in Fields B13 (2010) and C11 (2011) when soybean was planted at a higher population compared with control at lower soybean populations (control of 75% versus 64% and 72% versus 67%, resp., data not shown in tables).Table 3
The influence of herbicide resistance trait, soybean plant population, and preemergence herbicide on Palmer amaranth control 2 weeks after POST application in Field E2 during 2010.a
Soybean populationb
Preemergence herbicidesc
Palmer amaranth control
Herbicide resistance traitd
Glyphosate
Glufosinate
%
Low
None
43c
34d
Low
S-metolachlor
73b
74b
Low
S-metolachlor plus fomesafen
86a
82a
High
None
49c
49c
High
S-metolachlor
84ab
77b
High
S-metolachlor plus fomesafen
93a
97a
aMeans within a herbicide resistance trait followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P≤ 0.05.  Data are pooled over postemergence herbicides.
bLow and high soybean populations were 145,000 and 464,000, respectively.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
dGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 g ha−1 and 840 g ha−1, respectively.An interaction of PRE by POST by HRT was observed in Field E2 during 2010. In the absence of POST herbicides, Palmer amaranth control byS-metolachlor exceeded that of no PRE herbicide while S-metolachlor plus fomesafen was more effective than S-metolachlor alone (Table 4). Applying glufosinate or glyphosate after a residual PRE herbicide increased control over each POST herbicide alone.Table 4
The influence of herbicide resistance trait, preemergence herbicide, and postemergence herbicide on Palmer amaranth control 2 weeks after POST application in Field E2 during 2010.a
Preemergence herbicideb
Postemergence herbicide
Palmer amaranth control
Herbicide resistance traitc
Glufosinate
Glyphosate
%
None
No
0e
0d
S-metolachlor
No
33d
54b
S-metolachlor plus fomesafen
No
62b
46c
None
Yes
46c
59b
S-metolachlor
Yes
64ab
81a
S-metolachlor plus fomesafen
Yes
70a
85a
aMeans within a herbicide resistance trait followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P≤ 0.05.  Data are pooled over soybean population.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 kg ha−1, respectively.In Fields B13 during both years and Field C11 in 2011, the interaction of PRE and POST herbicide was significant for Palmer amaranth control.S-metolachlor plus fomesafen was more effective than S-metolachlor alone in 2 of 3 fields when POST herbicides were not applied and in 1 of 3 fields when POST herbicides were applied (Table 5). As expected, impact of PRE herbicides on Palmer amaranth control was minimized when POST herbicides were applied compared with no POST herbicide, although control increased in 2 of 3 fields when S-metolachlor plus fomesafen was applied compared with no PRE herbicide.Table 5
The influence of preemergence and postemergence herbicides on Palmer amaranth control 2 weeks after POST application.a
Preemergence herbicidesb
Postemergence herbicidesc
2010
2011
Field B13
Field B13
Field C11
%
None
No
6d
0b
14d
S-metolachlor
No
65c
92a
62c
S-metolachlor plus fomesafen
No
88ab
99a
90b
None
Yes
82b
93a
65c
S-metolachlor
Yes
89ab
100a
87b
S-metolachlor plus fomesafen
Yes
91a
100a
98a
aMeans within a year and field followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05. Data are pooled over herbicide resistance traits and soybean populations.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.The interaction of POST herbicides and HRT was significant for Palmer amaranth control in Field B13 during 2010. When pooled over soybean populations and PRE herbicides, Palmer amaranth control increased from 57% to 81% (glufosinate) and 43% to 94% (glyphosate) (data not shown in tables). Additionally, the value of PRE herbicides was noted for both HRT, withS-metolachlor plus fomesafen being the most effective PRE treatment (Table 6).Table 6
Palmer amaranth control 2 weeks after POST application as influenced by herbicide resistance trait and preemergence herbicide treatment during 2010 in Field B13.a
Preemergence herbicideb
Palmer amaranth control
Herbicide resistance traitc
Glufosinate
Glyphosate
%
None
49c
29c
S-metolachlor
78b
71b
S-metolachlor plus fomesafen
94a
93a
aMeans within a herbicide resistance trait followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.  Data are pooled over soybean population.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.In Field E3 during 2011, the interaction of soybean population, PRE herbicide, POST herbicide, and HRT was significant for Palmer amaranth control. Increasing the soybean population increased Palmer amaranth control from 83 to 85% to 98% whenS-metolachlor was applied alone (Table 7). This response was not observed when POST herbicides were applied. S-metolachlor plus fomesafen controlled Palmer amaranth at least 95% regardless of soybean population or POST herbicides.Table 7
The influence of herbicide resistance trait, soybean population, and preemergence and postemergence herbicides on Palmer amaranth control 2 weeks after post application in Field E3 during 2011.a
Soybean populationb
Palmer amaranth control
Herbicide application timing
Herbicide resistance traitd
Preemergencec
Postemergence
Glufosinate
Glyphosate
%
Low
None
No
0d
0d
Low
S-metolachlor
No
83bc
85bc
Low
S-metolachlor plus fomesafen
No
95ab
100a
Low
None
Yes
76c
78c
Low
S-metolachlor
Yes
98a
100a
Low
S-metolachlor plus fomesafen
Yes
100a
100a
High
None
No
0d
0d
High
S-metolachlor
No
98a
98a
High
S-metolachlor plus fomesafen
No
95ab
100a
High
None
Yes
85bc
85bc
High
S-metolachlor
Yes
100a
100a
High
S-metolachlor plus fomesafen
Yes
100a
100a
aMeans within a HRT followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P< 0.05.
bLow soybean population in Field E3 in 2011 was 193,000 plants m−2.  High soybean population in this field was 533,000 plants m−2.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
dGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.Soybean yield during 2010 was low compared with yield during 2011 due to dry conditions throughout most of the growing season. Soybean yield was higher in Fields E2 during 2010 and Field E3 during 2011 when the soybean population increased independent of PRE or POST herbicides and HRT (Table8). Applying S-metolachlor alone or with fomesafen increased yield over no PRE in each of these fields. Increasing the soybean population increased estimated economic return for Field E3 during 2011 (Table 9). Applying S-metolachlor or S-metolachlor plus fomesafen increased estimated economic return over no PRE treatment. Additionally, applying a POST herbicide increased estimated economic return (Table 9).Table 8
Soybean yield as influenced by soybean population and preemergence herbicide in Field E2 during 2010 and Field E3 during 2011.a
Treatment factor
2010
2011
Field E2
Field E3
kg ha−1
Soybean population
b
Low
200b
2210b
High
430a
3310a
Preemergence herbicide
c
None
250b
1910b
S-metolachlor
350a
3130a
S-metolachlor plus fomesafen
340a
3240a
aMeans within a field and treatment factor followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.
bLow soybean populations in Fields E2 (2010) and E3 (2011) were 145,000 and 193,000 plants ha−1, respectively.  High soybean populations in these respective fields were 464,000 and 533,000 plants ha−1.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.Table 9
Estimated economic return as influenced by soybean population, preemergence herbicide, or postemergence herbicide in Field E3 during 2011.a
Treatment factor
Economic return
$ ha−1
Soybean population
b
Low
585b
High
1017a
Preemergence herbicide
c
None
415b
S-metolachlor
962a
S-metolachlor plus fomesafen
1026a
Postemergence herbicide
d
No
562b
Yes
1039a
aMeans within a treatment factor followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.
bLow and high soybean populations in Field E3 during 2011 were 193,000 and 533,000  plants m−2, respectively.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
dPostemergence herbicides included glufosinate and glyphosate applied to appropriate herbicide-resistant cultivars at 560 and 840 g ha−1, respectively.Soybean yield and economic return were affected by the interaction of PRE and POST herbicides in Field B13 and C11 during 2011. In Field B13, a POST herbicide did not increase yield compared with no POST herbicides whenS-metolachlor or S-metolachlor plus fomesafen were applied (Table 10). The lowest yield in this field was noted when neither PRE nor POST herbicides were applied. In Field C11, yield was similar when S-metolachlor plus fomesafen were applied irrespective of POST herbicides or when a POST herbicide was applied irrespective of PRE herbicide treatment. Estimated economic return reflected soybean yield in Field C11. In Field B13, no difference in economic return was noted among treatments except when comparing the no PRE or POST treatment with S-metolachlor plus fomesafen.Table 10
Soybean yield and estimated economic return as influenced by preemergence and postemergence herbicide treatments during 2011 in Fields B13 and C11.a
Preemergence herbicidesb
Postemergence herbicidesc
Soybean yield
Economic return
Field B13
Field C11
Field B13
Field C11
kg ha−1
$ ha−1
None
No
720b
1620c
−112b
288c
S-metolachlor
No
930a
3420b
−57ab
1118b
S-metolachlor plus fomesafen
No
1120a
3990a
48a
1415a
None
Yes
940a
3820a
−31ab
1339a
S-metolachlor
Yes
1000a
4070a
−56ab
1408a
S-metolachlor plus fomesafen
Yes
1040a
4200a
−34ab
1471a
aMeans within a column followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.  Data are pooled over herbicide resistance traits and soybean populations.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cPostemergence herbicides included glufosinate and glyphosate applied to appropriate herbicide-resistant cultivars at 560 and 840 g ha−1, respectively.The interaction of soybean population by POST herbicide by HRT was significant for both yield and estimated economic return in Fields B13 and C11 in 2011. Increasing the soybean population and applying either glufosinate or glyphosate increased yield in both fields compared with yield at lower soybean populations with or without POST herbicides (Table11). Applying a POST herbicide in the low population or not including a POST herbicide in the higher population affected yield similarly in both fields. The lowest yield was noted in absence of POST herbicides at the lowest soybean population. Estimated economic return reflected yield in Field B13 for glyphosate and for glufosinate in Field C11. In Field B13 following glufosinate, the highest estimated economic return was noted after glufosinate was applied at the higher soybean population. Applying glyphosate resulted in similar estimated economic returns regardless of soybean population in Field C11. In the absence of POST herbicides, estimated economic return was greater with the higher soybean population.Table 11
Soybean yield and estimated economic return as influenced by herbicide resistance trait, soybean plant population, and postemergence herbicide treatment during 2011 in Fields B13 and C11.a
Soybean populationb
Post herbicidec
Soybean yield
Economic return
Herbicide resistance trait
Herbicide resistance trait
Field B13
Field C11
Field B13
Field C11
Glufosinate
Glyphosate
Glufosinate
Glyphosate
Glufosinate
Glyphosate
Glufosinate
Glyphosate
kg ha−1
$ ha−1
Low
No
1420c
540c
2860c
1980c
224c
−237c
924c
481c
Low
Yes
2180b
2290b
3790b
3620b
544b
601b
1326b
1263ab
High
No
2240b
2680b
3620b
3580b
507bc
705b
1201b
1153b
High
Yes
3720a
3850a
4560a
4150a
1195a
1255a
1625a
1408a
aMeans within a column followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P< 0.05. Data are pooled over preemergence herbicides.
bLow soybean populations in Fields B13 and C11 during 2011 was 221,000 and 208,000 plants m−2, respectively.  High soybean populations in these fields were 560,000 and 519,000 plants m−2, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.


## 3.1. Survey of Soybean Fields in North Carolina

The percentage of ha within the 2,512 fields with at least one Palmer amaranth plant in the field was estimated to be 39% with 61% not having Palmer amaranth present (data not shown in tables). The percentage of fields with between 1 and 50% of ha infested was 22% while 17% of fields were infested at a level of 51 to 100% by Palmer amaranth (data not presented in tables). Results from this survey, combined with those of Hoffner et al. [5], reveal the magnitude of Palmer amaranth infestation in North Carolina.


## 3.2. Interactions of Plant Population, Herbicide Resistant Trait, and Herbicide Program

Palmer amaranth population in absence of PRE herbicides ranged from 74 to 165 plants m−2 (Table 2). When S-metolachlor or S-metolachlor plus fomesafen was applied, the Palmer amaranth population decreased to 10 plants m−2 or less. No difference in Palmer amaranth density was noted when comparing S-metolachlor and S-metolachlor plus fomesafen. When activated by rainfall or irrigation, these herbicides often control Palmer amaranth for several weeks during the season [22].The interaction of soybean population by POST herbicide was significant for Palmer amaranth control in Field E2 during 2010. When pooled over HRT and PRE herbicides, control was 50 to 55% without POST herbicides (data not shown in tables). Applying a POST herbicide to the low seeding rate increased control to 81%, while the combination of the high seeding rate and POST herbicide controlled Palmer amaranth 94% (data not shown in tables). Soybean population also influenced the interaction of PRE herbicide and HRT in this field. In absence of PRE herbicides, Palmer amaranth was controlled more effectively when glufosinate was applied in the high population compared with the low population (Table3). The most effective control in this HRT was S-metolachlor plus fomesafen in the high population. Although PRE herbicides increased control when glyphosate was applied, control was not affected by soybean population (Table 3). In contrast to these results, soybean population did not interact with HRT or herbicide treatments in Fields B13 and C11. When pooled over HRT and PRE and POST herbicides, Palmer amaranth control was greater in Fields B13 (2010) and C11 (2011) when soybean was planted at a higher population compared with control at lower soybean populations (control of 75% versus 64% and 72% versus 67%, resp., data not shown in tables).Table 3
The influence of herbicide resistance trait, soybean plant population, and preemergence herbicide on Palmer amaranth control 2 weeks after POST application in Field E2 during 2010.a
Soybean populationb
Preemergence herbicidesc
Palmer amaranth control
Herbicide resistance traitd
Glyphosate
Glufosinate
%
Low
None
43c
34d
Low
S-metolachlor
73b
74b
Low
S-metolachlor plus fomesafen
86a
82a
High
None
49c
49c
High
S-metolachlor
84ab
77b
High
S-metolachlor plus fomesafen
93a
97a
aMeans within a herbicide resistance trait followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P≤ 0.05.  Data are pooled over postemergence herbicides.
bLow and high soybean populations were 145,000 and 464,000, respectively.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
dGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 g ha−1 and 840 g ha−1, respectively.An interaction of PRE by POST by HRT was observed in Field E2 during 2010. In the absence of POST herbicides, Palmer amaranth control byS-metolachlor exceeded that of no PRE herbicide while S-metolachlor plus fomesafen was more effective than S-metolachlor alone (Table 4). Applying glufosinate or glyphosate after a residual PRE herbicide increased control over each POST herbicide alone.Table 4
The influence of herbicide resistance trait, preemergence herbicide, and postemergence herbicide on Palmer amaranth control 2 weeks after POST application in Field E2 during 2010.a
Preemergence herbicideb
Postemergence herbicide
Palmer amaranth control
Herbicide resistance traitc
Glufosinate
Glyphosate
%
None
No
0e
0d
S-metolachlor
No
33d
54b
S-metolachlor plus fomesafen
No
62b
46c
None
Yes
46c
59b
S-metolachlor
Yes
64ab
81a
S-metolachlor plus fomesafen
Yes
70a
85a
aMeans within a herbicide resistance trait followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P≤ 0.05.  Data are pooled over soybean population.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 kg ha−1, respectively.In Fields B13 during both years and Field C11 in 2011, the interaction of PRE and POST herbicide was significant for Palmer amaranth control.S-metolachlor plus fomesafen was more effective than S-metolachlor alone in 2 of 3 fields when POST herbicides were not applied and in 1 of 3 fields when POST herbicides were applied (Table 5). As expected, impact of PRE herbicides on Palmer amaranth control was minimized when POST herbicides were applied compared with no POST herbicide, although control increased in 2 of 3 fields when S-metolachlor plus fomesafen was applied compared with no PRE herbicide.Table 5
The influence of preemergence and postemergence herbicides on Palmer amaranth control 2 weeks after POST application.a
Preemergence herbicidesb
Postemergence herbicidesc
2010
2011
Field B13
Field B13
Field C11
%
None
No
6d
0b
14d
S-metolachlor
No
65c
92a
62c
S-metolachlor plus fomesafen
No
88ab
99a
90b
None
Yes
82b
93a
65c
S-metolachlor
Yes
89ab
100a
87b
S-metolachlor plus fomesafen
Yes
91a
100a
98a
aMeans within a year and field followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05. Data are pooled over herbicide resistance traits and soybean populations.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.The interaction of POST herbicides and HRT was significant for Palmer amaranth control in Field B13 during 2010. When pooled over soybean populations and PRE herbicides, Palmer amaranth control increased from 57% to 81% (glufosinate) and 43% to 94% (glyphosate) (data not shown in tables). Additionally, the value of PRE herbicides was noted for both HRT, withS-metolachlor plus fomesafen being the most effective PRE treatment (Table 6).Table 6
Palmer amaranth control 2 weeks after POST application as influenced by herbicide resistance trait and preemergence herbicide treatment during 2010 in Field B13.a
Preemergence herbicideb
Palmer amaranth control
Herbicide resistance traitc
Glufosinate
Glyphosate
%
None
49c
29c
S-metolachlor
78b
71b
S-metolachlor plus fomesafen
94a
93a
aMeans within a herbicide resistance trait followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.  Data are pooled over soybean population.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.In Field E3 during 2011, the interaction of soybean population, PRE herbicide, POST herbicide, and HRT was significant for Palmer amaranth control. Increasing the soybean population increased Palmer amaranth control from 83 to 85% to 98% whenS-metolachlor was applied alone (Table 7). This response was not observed when POST herbicides were applied. S-metolachlor plus fomesafen controlled Palmer amaranth at least 95% regardless of soybean population or POST herbicides.Table 7
The influence of herbicide resistance trait, soybean population, and preemergence and postemergence herbicides on Palmer amaranth control 2 weeks after post application in Field E3 during 2011.a
Soybean populationb
Palmer amaranth control
Herbicide application timing
Herbicide resistance traitd
Preemergencec
Postemergence
Glufosinate
Glyphosate
%
Low
None
No
0d
0d
Low
S-metolachlor
No
83bc
85bc
Low
S-metolachlor plus fomesafen
No
95ab
100a
Low
None
Yes
76c
78c
Low
S-metolachlor
Yes
98a
100a
Low
S-metolachlor plus fomesafen
Yes
100a
100a
High
None
No
0d
0d
High
S-metolachlor
No
98a
98a
High
S-metolachlor plus fomesafen
No
95ab
100a
High
None
Yes
85bc
85bc
High
S-metolachlor
Yes
100a
100a
High
S-metolachlor plus fomesafen
Yes
100a
100a
aMeans within a HRT followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P< 0.05.
bLow soybean population in Field E3 in 2011 was 193,000 plants m−2.  High soybean population in this field was 533,000 plants m−2.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
dGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.Soybean yield during 2010 was low compared with yield during 2011 due to dry conditions throughout most of the growing season. Soybean yield was higher in Fields E2 during 2010 and Field E3 during 2011 when the soybean population increased independent of PRE or POST herbicides and HRT (Table8). Applying S-metolachlor alone or with fomesafen increased yield over no PRE in each of these fields. Increasing the soybean population increased estimated economic return for Field E3 during 2011 (Table 9). Applying S-metolachlor or S-metolachlor plus fomesafen increased estimated economic return over no PRE treatment. Additionally, applying a POST herbicide increased estimated economic return (Table 9).Table 8
Soybean yield as influenced by soybean population and preemergence herbicide in Field E2 during 2010 and Field E3 during 2011.a
Treatment factor
2010
2011
Field E2
Field E3
kg ha−1
Soybean population
b
Low
200b
2210b
High
430a
3310a
Preemergence herbicide
c
None
250b
1910b
S-metolachlor
350a
3130a
S-metolachlor plus fomesafen
340a
3240a
aMeans within a field and treatment factor followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.
bLow soybean populations in Fields E2 (2010) and E3 (2011) were 145,000 and 193,000 plants ha−1, respectively.  High soybean populations in these respective fields were 464,000 and 533,000 plants ha−1.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.Table 9
Estimated economic return as influenced by soybean population, preemergence herbicide, or postemergence herbicide in Field E3 during 2011.a
Treatment factor
Economic return
$ ha−1
Soybean population
b
Low
585b
High
1017a
Preemergence herbicide
c
None
415b
S-metolachlor
962a
S-metolachlor plus fomesafen
1026a
Postemergence herbicide
d
No
562b
Yes
1039a
aMeans within a treatment factor followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.
bLow and high soybean populations in Field E3 during 2011 were 193,000 and 533,000  plants m−2, respectively.
c
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
dPostemergence herbicides included glufosinate and glyphosate applied to appropriate herbicide-resistant cultivars at 560 and 840 g ha−1, respectively.Soybean yield and economic return were affected by the interaction of PRE and POST herbicides in Field B13 and C11 during 2011. In Field B13, a POST herbicide did not increase yield compared with no POST herbicides whenS-metolachlor or S-metolachlor plus fomesafen were applied (Table 10). The lowest yield in this field was noted when neither PRE nor POST herbicides were applied. In Field C11, yield was similar when S-metolachlor plus fomesafen were applied irrespective of POST herbicides or when a POST herbicide was applied irrespective of PRE herbicide treatment. Estimated economic return reflected soybean yield in Field C11. In Field B13, no difference in economic return was noted among treatments except when comparing the no PRE or POST treatment with S-metolachlor plus fomesafen.Table 10
Soybean yield and estimated economic return as influenced by preemergence and postemergence herbicide treatments during 2011 in Fields B13 and C11.a
Preemergence herbicidesb
Postemergence herbicidesc
Soybean yield
Economic return
Field B13
Field C11
Field B13
Field C11
kg ha−1
$ ha−1
None
No
720b
1620c
−112b
288c
S-metolachlor
No
930a
3420b
−57ab
1118b
S-metolachlor plus fomesafen
No
1120a
3990a
48a
1415a
None
Yes
940a
3820a
−31ab
1339a
S-metolachlor
Yes
1000a
4070a
−56ab
1408a
S-metolachlor plus fomesafen
Yes
1040a
4200a
−34ab
1471a
aMeans within a column followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P ≤ 0.05.  Data are pooled over herbicide resistance traits and soybean populations.
b
S-metolachlor and S-metolachlor plus fomesafen applied at 1500 and 1200 + 270 g ha−1, respectively.
cPostemergence herbicides included glufosinate and glyphosate applied to appropriate herbicide-resistant cultivars at 560 and 840 g ha−1, respectively.The interaction of soybean population by POST herbicide by HRT was significant for both yield and estimated economic return in Fields B13 and C11 in 2011. Increasing the soybean population and applying either glufosinate or glyphosate increased yield in both fields compared with yield at lower soybean populations with or without POST herbicides (Table11). Applying a POST herbicide in the low population or not including a POST herbicide in the higher population affected yield similarly in both fields. The lowest yield was noted in absence of POST herbicides at the lowest soybean population. Estimated economic return reflected yield in Field B13 for glyphosate and for glufosinate in Field C11. In Field B13 following glufosinate, the highest estimated economic return was noted after glufosinate was applied at the higher soybean population. Applying glyphosate resulted in similar estimated economic returns regardless of soybean population in Field C11. In the absence of POST herbicides, estimated economic return was greater with the higher soybean population.Table 11
Soybean yield and estimated economic return as influenced by herbicide resistance trait, soybean plant population, and postemergence herbicide treatment during 2011 in Fields B13 and C11.a
Soybean populationb
Post herbicidec
Soybean yield
Economic return
Herbicide resistance trait
Herbicide resistance trait
Field B13
Field C11
Field B13
Field C11
Glufosinate
Glyphosate
Glufosinate
Glyphosate
Glufosinate
Glyphosate
Glufosinate
Glyphosate
kg ha−1
$ ha−1
Low
No
1420c
540c
2860c
1980c
224c
−237c
924c
481c
Low
Yes
2180b
2290b
3790b
3620b
544b
601b
1326b
1263ab
High
No
2240b
2680b
3620b
3580b
507bc
705b
1201b
1153b
High
Yes
3720a
3850a
4560a
4150a
1195a
1255a
1625a
1408a
aMeans within a column followed by the same letter are not significantly different according to Fisher’s Protected LSD test at P< 0.05. Data are pooled over preemergence herbicides.
bLow soybean populations in Fields B13 and C11 during 2011 was 221,000 and 208,000 plants m−2, respectively.  High soybean populations in these fields were 560,000 and 519,000 plants m−2, respectively.
cGlufosinate and glyphosate applied to appropriate herbicide-resistant cultivar at 560 and 840 g ha−1, respectively.


## 4. Conclusions

Results from the survey during 2010 reveal the magnitude of Palmer amaranth infestation in soybean in North Carolina after weed control programs had been implemented (39% of fields surveyed). Palmer amaranth control did not differ in most cases when comparing glufosinate with glyphosate, most likely because frequency of glyphosate resistance was relatively low in these fields (10% or less) and because POST herbicides were applied when Palmer amaranth was relatively small based on manufacturer and Cooperative Extension recommendations. The combination ofS-metolachlor plus fomesafen PRE was often very effective in controlling Palmer amaranth in these experiments. The most consistent control across years and field sites was noted when PRE herbicides were followed by a POST herbicide. Currently, this strategy is used by many growers in North Carolina to control Palmer amaranth in soybean and other crops. However, concern has been expressed that even though herbicides that inhibit protoporphyrinogen oxidase, such as fomesafen, are effective in controlling Palmer amaranth, over reliance on herbicides with this mode of action could lead to resistance [23]. Therefore, cultural practices such as increased seeding rate, as investigated in this study, may become increasingly important in managing Palmer amaranth and other weeds where concern over herbicide resistance exists [14]. Although some variation was noted among combinations of HRT and PRE herbicides in our study, a higher soybean population often increased Palmer amaranth control, soybean yield, and estimated economic return. Even though production costs are higher with elevated seeding rates, results suggest that seeding rates that are higher than those necessary under weed-free conditions to optimize yield can be established without a negative impact on economic return when Palmer amaranth is present. While not documented in these studies, minimizing Palmer amaranth reproduction is an important management practice in soybean and other crops, especially in fields where herbicide resistance is present.



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*Source: 947395-2012-09-19.xml*