# Exposure Assessment Based Recommendations to Improve Nanosafety at Nanoliposome Production Sites

Authors: Christophe Bressot; Neeraj Shandilya; Eugénia Sofia da Costa Nogueira; Artur Cavaco-Paulo; Martin Morgeneyer; Olivier Le Bihan; Olivier Aguerre-Chariol
Journal: Journal of Nanomaterials
 (2015)

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## Abstract

The NANOFOL  concept aims at creating nanodevices containing a drug for inflammatory disorder treatment. This paper provides recommendations for nanosafety based on a measurement campaign which aimed at identifying exposure risks with respect to two specific phases of the product’s lifecycle, that is, production of the device and its waste management. The nanoparticle’s presence both in air and in liquid phase was studied. While no emissions were detected during the production period, many recommendations have been made, particularly regarding the nanowaste treatment, based on nanosafety guidelines.


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## Body

## 1. Introduction

NANOFOL [1] was a European Project supported through the Seventh Framework Programme for Research and Technological Development. It aims at improving the treatment of chronic inflammatory diseases by the development and production of nanobiodevices (i.e., nanoliposomes) in nanosafe conditions. Nanoliposomes are nanosized vesicles made mainly of phospholipids, able to enclose molecules, and used as device to facilitate drug transport and penetration into cells. As a result, a part of this project is devoted to nanosafety management with different operations which are used to deal with nanorisk during production and waste management. Nanosafety is a function of two distinct factors, exposure (of workers) and hazard (toxicity/ecotoxicity of nanomaterial). One of the most widely applied standards for exposure assessment at workplaces is EN 689, 1995 [2]. This standard enlists the critical points that need to be addressed for a fair evaluation of these two factors. Table 1 summarizes these points. In addition, the standard also enlists two preventive measures to deal for the safety of workers.Table 1
Factors to be considered  in exposure assessment as enlisted by NF EN 689, 1995.
Description
Points to be addressed
Hazard
(a) Number of sources emitting pollution
(b) Size of the production in relation to production capacity
(c) Size of each of the sources
(d) Type and location of each source
Probability of exposure
(a) Dispersion of pollutants by air movement
(b) Proximity to sources/confinement barrier
(c) Time spent in a location
Preventive measures
(a) Type and efficacy of evacuation and ventilation systems
(b) Personal working habitsAs far as the handling and manipulation of the nanosuspensions are concerned, there is no such standard available as per the knowledge of the authors. Moreover, the two abovementioned factors exposure and hazards are still rather poorly understood in the context of nanosuspensions [3]. Nanosuspensions are biphasic systems consisting of pure drug particles (or nanostructured vesicles like liposomes) dispersed in an aqueous solvent, stabilized by surfactants. With the ability to improve the stability as well as the bioavailability of poorly soluble drugs and easiness to prepare, these nanosuspensions are becoming a vital technology in the pharmaceutical sector [4]. Hence, it is critical to control the exposure and hazards associated during their manipulation for their sustainable development. This has been a subject of interest of some European Community regulations, for example, the EC directives 1998 [5] and EP and EC directives 2004 [6]. The main application of these nanosuspensions is in the production of nanomedicines. The nanostructured compounds used in nanomedicine are rarely reported as a main risk [7–9]. Nanomedicine is a relatively new topic in the nanorisk framework and the amount of chemical compounds involved seems to be low compared with industrial products containing nanomaterials [10].Elsaesser and Howard [11] reported the need to develop a regulatory framework based on objective scientific research which will limit human exposure to safe levels of the unwanted engineered nanomaterials in the environment. This approach requires a different framework which balances the therapeutic benefit against the potential risk to worker and the environment. In spite of clear improvements of risk assessment to drive nanodevice conception to safer drug delivery [12–18], to our knowledge no studies concerning nanomedicine safety at the workplace have been published.However nanomedicine recommendations in healthcare sector [19] or general guideline on nanosafety [20–23] can also be applied in the context of nanomedicine production workplace. In case of nanosuspension manipulation, converging recommendations consist consequently to handle liquid dispersions with adapted personal protective equipment (e.g., nitrile gloves, lab coats, and safety glasses) under a fume hood. The complementary works that have studied the nanosuspensions report that the hazards related to nanosuspension are not only influenced by the particle nature but also by the dispersant, for example, [24]. Here, the main recommendation focuses on the manipulated quantities more than one litre and the nature of the used dispersant (i.e., flammable, toxic, etc.). In case of nanoparticle production under high pressure conditions, a significant risk of nanoparticle aerosolization has been identified. Lee et al. [25] and Johnson et al. [26] reported aerosolization during energetic process using carbon nanotube suspensions. It is the understanding of the authors that such a behavior of carbon nanotube suspensions can also be expected in case of nanoliposome suspensions. Daigle et al. [27] asserted that the likely exposure route to engineered nanomaterials is through inhalation, though ingestion or dermal penetration may also occur [28–30].The exposure measurement in presence of nanomaterial use implicates an evaluation of data gathering to estimate the nanorisk or the critical periods which necessitate a measurement campaign. These measurements are intended, whether a nanorisk is identified, to provide evidence for the nanoparticle presence within the breathing zone of the workers during the period of nanomaterial use. An appropriate method to perform an exposure measurement campaign consists of an assessment campaign with a particle counter and sizer and, if necessary, a complementary campaign of measurements [31–34].The present paper assesses the potential of this method in an industry-like laboratory that produces nanoliposome liquid suspensions. It aims at assessing the exposure to nanoparticles and at providing recommendations with respect to this. The three steps of this assessment have been(i)
to identify and quantify the sources of nanostructured particle emissions in the production facility,(ii)
to characterize the emitted aerosol particles in terms of number concentration,(iii)
to manage the nanoliposome waste in the production facility by cracking the inherent nanostructure of the membrane,(iv)
to propose recommendations by means of concrete measures for the alleviation of the potential exposure in case of an accident.A particle counter is used to quantify the number concentration of the aerosol particles with an aerosol sampler to characterize their shape and elemental composition. The latter also allows for identifying the probable nanoliposome emissions with certainty as a particle counter cannot differentiate between the process and background related aerosol particles.


## 2. Materials and Methods

### 2.1. Measurement Strategy

Various attempts have been made in the past to develop a comprehensive assessment strategy for the measurement campaigns at workplaces or production sites [34–36]. The design of the assessment strategy relies on the choice of the exposure measurement instruments which in turn depends upon the purpose of the measurement (as outlined by [37, 38]). Besides this, the extensitivity, longevity, and background aerosols ladening (originating from the work environment or the process itself) of the assessment procedure and the production site’s structure or architecture also play key roles while designing the strategy. In the present work, the investigated production site is the laboratory of University of Minho (UMINHO) on the Campus of Azurém of Guimarães (Portugal) which produces the liquid suspension of the nanoliposomes for manufacturing nanodrugs which are used for various inflammatory disorder treatments. As shown in Figure 1, the laboratory is divided in two parts. The first part is used to store samples and chemical compounds in a fridge and the second area is used to handle compounds and produce nanoliposomes. All sorts of product handling or processing are performed inside the conformity of a fume hood or directly on the laboratory benches (marked as A, B, and C). To reduce operators’ risks of exposure, the two key means of action are the air flow and protective equipment. The ventilation controls were kept switched off during the measurement campaign and the air flow was consequently natural in the laboratory. The protective equipment used for the operator’s protection is nitrile gloves, safety goggles, cotton lab coat, and mask.Figure 1
Nanoliposome liquid suspension producing laboratory floor plan.The different steps during the production process are presented in Figure2. The first step consists in weighing various chemical compounds as dioleoylphosphatidylethanolamine (DOPE), cholesterol (CH), or (N-(carbonylmethoxypolyethylene glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine) DSPE-MPEG using a digital balance and placing them under the fume hood. In the second step of handling, these chemical compounds are mixed with chloroform in definite ratios inside the fume hood 1.Figure 2
A series of steps involved during the production process of nanoliposome liquid suspensions; the last two steps, that is, extrusion and purification of these suspensions, are considered to be the steps during which possible emissions of nanoliposomes might occur.The chloroform is then evaporated inside fume hood 2 during the third step. During fourth step, a lipid film is prepared on a round bottom flask to prepare for the nanoliposomes production. This is performed mainly on the laboratory bench A; see Figure1. Until this step, no nanostructured product is present and consequently there is no risk of exposure to nanoliposomes. The dried lipid film is treated with phosphate buffered saline (PBS) to prepare its suspension which is followed by the suspension’s vortex mixing, to yield multilamellar vesicles (MLVs) the first step when the nanoliposomes are manufactured. Consequently, this could be the first moment when their emission might occur. The extrusion step, through polycarbonate filters, uses a pressure system, to form large unilamellar vesicles. Following extrusion, these nanoliposomes are purified or separated from the residue by passing the mixture through a SPE column. Like extrusion, this step could also be a potential source of nanoliposomes emission and could drive to an inhalation or dermal exposure risk. Both of these steps are carried out at A and C places (Figure 1). The DOPE-containing liposomes formulation is used to stabilize the bilayer form through the addition of a cleavable lipid derivative of polyethylene glycol (DSPE-MPEG). This new nanobiodevice has been developed by UMINHO and tested by other partners and seems to be the best candidate to be used [3]. Approximately a duration of 1 hour 30 minutes is required to produce liquid suspension of 15 mL containing nanoliposomes with a liposomal formulation DOPE/CH/DSPE-MPEG concentration of 11.9 mM at the purification step. The production batch can be repeated 5 times in a day and necessitates only one worker.Hence, we consider these two stages, that is, extrusion and purification to be sole potential periods of emission. As a result, the particle counting and sampling should be done within this zone only.Further to the risk of emission to the air, the liquid suspensions represent an inherent possible accidental source of nanoparticles.


### 2.2. Nanoliposome Liquid Suspension Analysis

Before starting the measurement campaign, a characterization of the final product, that is, nanoliposome liquid suspension, was carried out. In Figure3, scattering intensity-weighted particle size distribution of the nanoliposome liquid suspension, manufactured by producer, is shown. It was carried out using Zetasizer Nano ZS (Malvern Instruments) by means of Dynamic Light Scattering (DLS). A monodispersed distribution of the nanoliposomes, with a mode just below 100 nm, can be observed.Figure 3
Particle size distribution of the liposome liquid suspension obtained using DLS.For the Transmission Electron Microcopy (TEM, Model CM12; Philips, Netherlands; shown in Figure4), 8 μL droplet from this suspension was deposited on a TEM copper mesh grid (Model S162; Formvar/Carbon, Quantifoil Micro Tools GmbH, Germany). The mesh grid was made hydrophilic by its plasma treatment, 0.1 mbar, 45 mA, and 3 min (Model K100X, Glow Discharge, Emitech, Quorum Technologies Ltd., UK), prior to drop deposition. After the deposition, the grid was then allowed to dry in a closed chamber so that the aqueous content gets evaporated and the nanoliposomes rest deposited on the grid. Since many nanoliposomes get destroyed during drying, a lot of disrupted membranes are observed in TEM microscopy (examples marked by solid black arrows in Figure 4). Fortunately, the nanoliposomes provided by UMINHO are so stable that some of them are still observable after drying (examples marked by dashed white arrows in Figure 4). The size of the nanoliposomes, in Figure 4, is in a size range of 75 nm to ~1 μm with small vesicles in the range of 75 to 150 nm. These observations are consistent with the previous observations of DLS sizing (Figure 3). The Energy Dispersive X-ray (EDS) analysis of the droplet revealed the chemical elements present in the membrane. An example is shown in Figure 5. Most of the elements found are ubiquitous like Cl, C, Na, Al, Si, and K. To be certain of the nature of the sampled aerosol particles during exposure analysis, the need of a tracer element is indispensable since this distinguishes the nanoliposomes from the background particles. For this, the tracer element (i) should be present inside the vesicular membrane, (ii) should not to be present in the laboratory environment, and (iii) should be easily identifiable. The only element satisfying these criteria is phosphorus (P) which is present in the phospholipid membranes. Another criterion that can be taken into account in order to further confirm the release of aerosolized nanoliposome is its spherical shape as most of the background particles are irregularly shaped.Figure 4
TEM image of the liposomes obtained after drying a deposited drop of its liquid suspension on a grid; examples of liposomes with disrupted membranes are marked with black arrows and those with stable membrane are marked with white arrows.Figure 5
Elemental composition of one nanoliposome with C, Na, Cl, K, Al, and P.


### 2.3. Exposure Analysis

The choice of the exposure measurement device was guided by two factors: (i) portability and easiness to manage; (ii) ability to measure nanosized particles. Considering these two factors, the exposure analysis during the production of the nanoliposomes was carried out using a Condensation Particle Counter (CPC 3007, TSI Inc.) which measures particles in the size range from 10 nm to 1μm. The sampling time was kept at 1 s with an air flow rate equal to 0.7 L/min. The CPC 3007 was complemented with a Mini Particle Sampler (MPS; Ecomesure) [39]. It is used for aerosol particles collection through filtration technique on porous copper mesh grids (Model S143-3; Quantifoil Micro Tools GmbH Germany). The use of a sampler is imperative here because a particle counter is generally insensitive to particle source or composition and makes it difficult to differentiate between background and process-related aerosol particles. Once collected, these particles then can be analyzed through TEM in order to confirm their nature, that is, background particles or nanoliposomes.


## 2.1. Measurement Strategy

Various attempts have been made in the past to develop a comprehensive assessment strategy for the measurement campaigns at workplaces or production sites [34–36]. The design of the assessment strategy relies on the choice of the exposure measurement instruments which in turn depends upon the purpose of the measurement (as outlined by [37, 38]). Besides this, the extensitivity, longevity, and background aerosols ladening (originating from the work environment or the process itself) of the assessment procedure and the production site’s structure or architecture also play key roles while designing the strategy. In the present work, the investigated production site is the laboratory of University of Minho (UMINHO) on the Campus of Azurém of Guimarães (Portugal) which produces the liquid suspension of the nanoliposomes for manufacturing nanodrugs which are used for various inflammatory disorder treatments. As shown in Figure 1, the laboratory is divided in two parts. The first part is used to store samples and chemical compounds in a fridge and the second area is used to handle compounds and produce nanoliposomes. All sorts of product handling or processing are performed inside the conformity of a fume hood or directly on the laboratory benches (marked as A, B, and C). To reduce operators’ risks of exposure, the two key means of action are the air flow and protective equipment. The ventilation controls were kept switched off during the measurement campaign and the air flow was consequently natural in the laboratory. The protective equipment used for the operator’s protection is nitrile gloves, safety goggles, cotton lab coat, and mask.Figure 1
Nanoliposome liquid suspension producing laboratory floor plan.The different steps during the production process are presented in Figure2. The first step consists in weighing various chemical compounds as dioleoylphosphatidylethanolamine (DOPE), cholesterol (CH), or (N-(carbonylmethoxypolyethylene glycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine) DSPE-MPEG using a digital balance and placing them under the fume hood. In the second step of handling, these chemical compounds are mixed with chloroform in definite ratios inside the fume hood 1.Figure 2
A series of steps involved during the production process of nanoliposome liquid suspensions; the last two steps, that is, extrusion and purification of these suspensions, are considered to be the steps during which possible emissions of nanoliposomes might occur.The chloroform is then evaporated inside fume hood 2 during the third step. During fourth step, a lipid film is prepared on a round bottom flask to prepare for the nanoliposomes production. This is performed mainly on the laboratory bench A; see Figure1. Until this step, no nanostructured product is present and consequently there is no risk of exposure to nanoliposomes. The dried lipid film is treated with phosphate buffered saline (PBS) to prepare its suspension which is followed by the suspension’s vortex mixing, to yield multilamellar vesicles (MLVs) the first step when the nanoliposomes are manufactured. Consequently, this could be the first moment when their emission might occur. The extrusion step, through polycarbonate filters, uses a pressure system, to form large unilamellar vesicles. Following extrusion, these nanoliposomes are purified or separated from the residue by passing the mixture through a SPE column. Like extrusion, this step could also be a potential source of nanoliposomes emission and could drive to an inhalation or dermal exposure risk. Both of these steps are carried out at A and C places (Figure 1). The DOPE-containing liposomes formulation is used to stabilize the bilayer form through the addition of a cleavable lipid derivative of polyethylene glycol (DSPE-MPEG). This new nanobiodevice has been developed by UMINHO and tested by other partners and seems to be the best candidate to be used [3]. Approximately a duration of 1 hour 30 minutes is required to produce liquid suspension of 15 mL containing nanoliposomes with a liposomal formulation DOPE/CH/DSPE-MPEG concentration of 11.9 mM at the purification step. The production batch can be repeated 5 times in a day and necessitates only one worker.Hence, we consider these two stages, that is, extrusion and purification to be sole potential periods of emission. As a result, the particle counting and sampling should be done within this zone only.Further to the risk of emission to the air, the liquid suspensions represent an inherent possible accidental source of nanoparticles.


## 2.2. Nanoliposome Liquid Suspension Analysis

Before starting the measurement campaign, a characterization of the final product, that is, nanoliposome liquid suspension, was carried out. In Figure3, scattering intensity-weighted particle size distribution of the nanoliposome liquid suspension, manufactured by producer, is shown. It was carried out using Zetasizer Nano ZS (Malvern Instruments) by means of Dynamic Light Scattering (DLS). A monodispersed distribution of the nanoliposomes, with a mode just below 100 nm, can be observed.Figure 3
Particle size distribution of the liposome liquid suspension obtained using DLS.For the Transmission Electron Microcopy (TEM, Model CM12; Philips, Netherlands; shown in Figure4), 8 μL droplet from this suspension was deposited on a TEM copper mesh grid (Model S162; Formvar/Carbon, Quantifoil Micro Tools GmbH, Germany). The mesh grid was made hydrophilic by its plasma treatment, 0.1 mbar, 45 mA, and 3 min (Model K100X, Glow Discharge, Emitech, Quorum Technologies Ltd., UK), prior to drop deposition. After the deposition, the grid was then allowed to dry in a closed chamber so that the aqueous content gets evaporated and the nanoliposomes rest deposited on the grid. Since many nanoliposomes get destroyed during drying, a lot of disrupted membranes are observed in TEM microscopy (examples marked by solid black arrows in Figure 4). Fortunately, the nanoliposomes provided by UMINHO are so stable that some of them are still observable after drying (examples marked by dashed white arrows in Figure 4). The size of the nanoliposomes, in Figure 4, is in a size range of 75 nm to ~1 μm with small vesicles in the range of 75 to 150 nm. These observations are consistent with the previous observations of DLS sizing (Figure 3). The Energy Dispersive X-ray (EDS) analysis of the droplet revealed the chemical elements present in the membrane. An example is shown in Figure 5. Most of the elements found are ubiquitous like Cl, C, Na, Al, Si, and K. To be certain of the nature of the sampled aerosol particles during exposure analysis, the need of a tracer element is indispensable since this distinguishes the nanoliposomes from the background particles. For this, the tracer element (i) should be present inside the vesicular membrane, (ii) should not to be present in the laboratory environment, and (iii) should be easily identifiable. The only element satisfying these criteria is phosphorus (P) which is present in the phospholipid membranes. Another criterion that can be taken into account in order to further confirm the release of aerosolized nanoliposome is its spherical shape as most of the background particles are irregularly shaped.Figure 4
TEM image of the liposomes obtained after drying a deposited drop of its liquid suspension on a grid; examples of liposomes with disrupted membranes are marked with black arrows and those with stable membrane are marked with white arrows.Figure 5
Elemental composition of one nanoliposome with C, Na, Cl, K, Al, and P.


## 2.3. Exposure Analysis

The choice of the exposure measurement device was guided by two factors: (i) portability and easiness to manage; (ii) ability to measure nanosized particles. Considering these two factors, the exposure analysis during the production of the nanoliposomes was carried out using a Condensation Particle Counter (CPC 3007, TSI Inc.) which measures particles in the size range from 10 nm to 1μm. The sampling time was kept at 1 s with an air flow rate equal to 0.7 L/min. The CPC 3007 was complemented with a Mini Particle Sampler (MPS; Ecomesure) [39]. It is used for aerosol particles collection through filtration technique on porous copper mesh grids (Model S143-3; Quantifoil Micro Tools GmbH Germany). The use of a sampler is imperative here because a particle counter is generally insensitive to particle source or composition and makes it difficult to differentiate between background and process-related aerosol particles. Once collected, these particles then can be analyzed through TEM in order to confirm their nature, that is, background particles or nanoliposomes.


## 3. Exposure Assessment Results

The exposure assessment with respect to the production phase of the product was carried out in three steps:(a)
Preliminary or “naked eye” evaluation of the exposure on the basis of the points enlisted in Table1.(b)
Measurement and analysis of the background aerosol particles with no production activity.(c)
Measurement and analysis of the aerosol particles during production activity.Following this investigation, the waste management phase needed to be considered; see Section4.


### 3.1. Data Evaluation Based on EN 689

The points enlisted in Table1 were analyzed for the present production site and observations were made corresponding to each point. Table 2 summarizes the results.Table 2
Responses to the factors considered (field study) [2].
Description
Points to be addressed
Observations on the production site
Hazard
(a) Number of sources emitting pollution
Three sources: extruder, storage, and garbage
(b) Size of the production and waste generation in relation to the production capacity
A daily intensive production (5 batches per day) produces 75 mL.  If we consider 260 days/year of production, the maximum total production is 19,500 mL/year. The phospholipid concentration in dioleoylphosphatidylethanolamine (DOPE) is 6.43 mM (4.78 mg/mL) in the final productThe waste consists of residues from water based solution which is used to clean the extruder and tools
(c) Size of each of the sources
During extrusion and storage phases, 15 mL of nanoliposome suspension is dealt with
(d) Type and location of each source
(a) Nanoliposomes production on the three benches A, B, and C,(b) fridge, (c) garbage (Figure 1)
Probability of exposure
(a) Dispersion of pollutants by air movement
There was neither any measured dispersion nor any ATEX risk identified
(b) Proximity to sources/confinement barrier
Handling without hood
(c) Time passed in a location
10 min for handling the nanoliposome liquid suspensions, 60 min for their extrusion, and 30 min for their purification
Preventive measures
(a) Type and efficacy of evacuation and ventilation systems
Presence of hood for chemical and biological products; no measured dispersion
(b) Personal working habits
In accordance with the chemical/biological riskNo direct account taken yet of nanoriskIn Table2, two main exposure sources are mentioned: nanoliposomesproduction and theirwaste disposal. This part of the campaign has provided a visual perception of the workplace (process and work practices) to define the sampling points which could be the potential sources of emission during production. At the same time, the information collected during this part has also provided useful inputs, like details regarding ventilation and personal protection equipment, to carry out the risk assessment studies. Since there is high nanoliposome stability after drying, therefore, an easy and efficient management of their safe disposal is equally crucial.


### 3.2. Measurement Results

In Figure6, the aerosol particles concentration, obtained using CPC 3007, is shown for the background particles as well as for the particles emitted during extrusion and purification steps of the nanoliposomes production. As already mentioned in Table 2, the duration of the extrusion and purification steps are 60 min and 30 min, respectively. For the background particles, the mean concentration ≈17,000 #/cm3 with a standard deviation ≈2,200 #/cm3 which proves relatively high fluctuations in the laboratory air (see Table 3). Towards the end, an increase in the concentration can be observed (≈30,000 #/cm3). Such fluctuations are very frequent to be observed at any production site, especially when the source of ventilation is natural, as in the present case.Table 3
Statistic data and the observations from the TEM analysis obtained during the three measurement steps.
Mean conc.
Min
Max
Std. Dev.
TEM analysis
(#/cm3)
(#/cm3)
(#/cm3)
(#/cm3)
Background
16 764
13 469
29 818
2 188
Oil droplets, soot building debris
Extrusion
11 250
9 189
27 388
822
Oil droplets or soot; no nanoliposomes detected
Purification
38 583
21 440
72 180
7 375
Oil droplets, soot building debris; no nanoliposomes detectedFigure 6
(a) Number concentration of the background particles and (b) the ambient airborne particles in the vicinity of the fume hood 1 (breathing zone of the worker) during the extrusion (green color) and purification (black color) of the nanoliposome liquid suspensions.
(a)
(b)The TEM analysis of the background particles, sampled att=10 : 46, reveals the typical nature of any particle usually found in ambient air, that is, droplets of oil, soot (Figure 7(a)), and rare cement-type materials (Figure 7(b)). The overview shows a moderately loaded grid with a common duration sampling of 6 minutes where drops of oil seem to be predominant.Figure 7
(a) Overview of the sampled particles from the background; (b) typical cement-type debris present in the ambient air.
(a)
(b)Coming back to Figure6, with respect to the background particle, no significant change in the number concentration can be observed during extrusion. The TEM images of the aerosol particles, sampled during this step, are shown in Figures 8(a) and 8(b). Like number concentration, there is no change in their nature too. Same type of oil droplets (Figure 8(a)) or soot (Figure 8(b)) was observed during extrusion phase.Figure 8
Typical TEM image examples of (a) soot and oil droplets (main compound carbon and iron) and (b) soot (main compound carbon) reaped during the airborne particles collection while the worker filters the liquid suspensions.
(a)
(b)In contrast with the extrusion, the purification step does give rise to an increase in the number concentration (mean concentration ≈39,000 #/cm3) (Figure 6). This is the highest mean value obtained during the campaign with maximum concentration reaching 72,000 #/cm3. Different peaks in the number concentration curve can be attributed to the opening/closing of the doors in the weighing room which perturbs the air flow of the laboratory. The absence of nanoliposomes emission during purification is confirmed from the TEM images of the sampled aerosol particles (Figures 9(a) and 9(b)) which like earlier TEM images also contain soot and oil droplets.Figure 9
(a) Typical overview of TEM grid with oil droplets and soot; (b) presence of big cement-type material on the TEM grid during the purification step.
(a)
(b)Hence, in all the activities during production process, the phosphorus element is never detected in the particles tested on the grids. Therefore, despite the intensive research and data collection during both particle counting and aerosols sampling in the laboratory, it has not been possible to identify nanoliposomes observed in Figure4. All the peaks in the number concentration of the aerosol particles, during either extrusion or purification, are associated with perturbations in the laboratory’s air flow due to the incidents like the turning on/off of the fume hood, doors opening/close, and so forth.


## 3.1. Data Evaluation Based on EN 689

The points enlisted in Table1 were analyzed for the present production site and observations were made corresponding to each point. Table 2 summarizes the results.Table 2
Responses to the factors considered (field study) [2].
Description
Points to be addressed
Observations on the production site
Hazard
(a) Number of sources emitting pollution
Three sources: extruder, storage, and garbage
(b) Size of the production and waste generation in relation to the production capacity
A daily intensive production (5 batches per day) produces 75 mL.  If we consider 260 days/year of production, the maximum total production is 19,500 mL/year. The phospholipid concentration in dioleoylphosphatidylethanolamine (DOPE) is 6.43 mM (4.78 mg/mL) in the final productThe waste consists of residues from water based solution which is used to clean the extruder and tools
(c) Size of each of the sources
During extrusion and storage phases, 15 mL of nanoliposome suspension is dealt with
(d) Type and location of each source
(a) Nanoliposomes production on the three benches A, B, and C,(b) fridge, (c) garbage (Figure 1)
Probability of exposure
(a) Dispersion of pollutants by air movement
There was neither any measured dispersion nor any ATEX risk identified
(b) Proximity to sources/confinement barrier
Handling without hood
(c) Time passed in a location
10 min for handling the nanoliposome liquid suspensions, 60 min for their extrusion, and 30 min for their purification
Preventive measures
(a) Type and efficacy of evacuation and ventilation systems
Presence of hood for chemical and biological products; no measured dispersion
(b) Personal working habits
In accordance with the chemical/biological riskNo direct account taken yet of nanoriskIn Table2, two main exposure sources are mentioned: nanoliposomesproduction and theirwaste disposal. This part of the campaign has provided a visual perception of the workplace (process and work practices) to define the sampling points which could be the potential sources of emission during production. At the same time, the information collected during this part has also provided useful inputs, like details regarding ventilation and personal protection equipment, to carry out the risk assessment studies. Since there is high nanoliposome stability after drying, therefore, an easy and efficient management of their safe disposal is equally crucial.


## 3.2. Measurement Results

In Figure6, the aerosol particles concentration, obtained using CPC 3007, is shown for the background particles as well as for the particles emitted during extrusion and purification steps of the nanoliposomes production. As already mentioned in Table 2, the duration of the extrusion and purification steps are 60 min and 30 min, respectively. For the background particles, the mean concentration ≈17,000 #/cm3 with a standard deviation ≈2,200 #/cm3 which proves relatively high fluctuations in the laboratory air (see Table 3). Towards the end, an increase in the concentration can be observed (≈30,000 #/cm3). Such fluctuations are very frequent to be observed at any production site, especially when the source of ventilation is natural, as in the present case.Table 3
Statistic data and the observations from the TEM analysis obtained during the three measurement steps.
Mean conc.
Min
Max
Std. Dev.
TEM analysis
(#/cm3)
(#/cm3)
(#/cm3)
(#/cm3)
Background
16 764
13 469
29 818
2 188
Oil droplets, soot building debris
Extrusion
11 250
9 189
27 388
822
Oil droplets or soot; no nanoliposomes detected
Purification
38 583
21 440
72 180
7 375
Oil droplets, soot building debris; no nanoliposomes detectedFigure 6
(a) Number concentration of the background particles and (b) the ambient airborne particles in the vicinity of the fume hood 1 (breathing zone of the worker) during the extrusion (green color) and purification (black color) of the nanoliposome liquid suspensions.
(a)
(b)The TEM analysis of the background particles, sampled att=10 : 46, reveals the typical nature of any particle usually found in ambient air, that is, droplets of oil, soot (Figure 7(a)), and rare cement-type materials (Figure 7(b)). The overview shows a moderately loaded grid with a common duration sampling of 6 minutes where drops of oil seem to be predominant.Figure 7
(a) Overview of the sampled particles from the background; (b) typical cement-type debris present in the ambient air.
(a)
(b)Coming back to Figure6, with respect to the background particle, no significant change in the number concentration can be observed during extrusion. The TEM images of the aerosol particles, sampled during this step, are shown in Figures 8(a) and 8(b). Like number concentration, there is no change in their nature too. Same type of oil droplets (Figure 8(a)) or soot (Figure 8(b)) was observed during extrusion phase.Figure 8
Typical TEM image examples of (a) soot and oil droplets (main compound carbon and iron) and (b) soot (main compound carbon) reaped during the airborne particles collection while the worker filters the liquid suspensions.
(a)
(b)In contrast with the extrusion, the purification step does give rise to an increase in the number concentration (mean concentration ≈39,000 #/cm3) (Figure 6). This is the highest mean value obtained during the campaign with maximum concentration reaching 72,000 #/cm3. Different peaks in the number concentration curve can be attributed to the opening/closing of the doors in the weighing room which perturbs the air flow of the laboratory. The absence of nanoliposomes emission during purification is confirmed from the TEM images of the sampled aerosol particles (Figures 9(a) and 9(b)) which like earlier TEM images also contain soot and oil droplets.Figure 9
(a) Typical overview of TEM grid with oil droplets and soot; (b) presence of big cement-type material on the TEM grid during the purification step.
(a)
(b)Hence, in all the activities during production process, the phosphorus element is never detected in the particles tested on the grids. Therefore, despite the intensive research and data collection during both particle counting and aerosols sampling in the laboratory, it has not been possible to identify nanoliposomes observed in Figure4. All the peaks in the number concentration of the aerosol particles, during either extrusion or purification, are associated with perturbations in the laboratory’s air flow due to the incidents like the turning on/off of the fume hood, doors opening/close, and so forth.


## 4. Nanowaste Management

Nanowaste management has been emphasized recently due to free or agglomerated nanoparticle release characterization in smoke or incineration residue [40, 41]. In case of nanoparticles present in sewage due, for example, to feces/urine, one part of this pollution is detected in the treated effluent [42–44].In the framework of this study, the experimentally simulated waste treatment consisted of a dilution by PBS followed by thermal cracking to manage the nanorisk posed by the waste. To test the destruction of DOPE formulations of nanoliposome, we diluted the 20μL sample of the suspension provided by UMINHO into 10 mL of PBS (1/50 v/v) at ambient temperature, followed by heating at 60°C for 10 hours. Consequently, there was a complete destruction of the suspension sample. To illustrate this, Figures 10(a), 10(b), and 10(c) show a shift of the size mode from 100 nm (Figure 10(a)) to around 1000 nm (Figure 10(c)) which shows a complete destruction of the nanostructure of the nanoliposome.Figure 10
(a) The initial particle size distribution of the diluted nanoliposome liquid suspension provided by UMINHO (20μL of nanoliposome suspension in 10 mL of PBS) placed in contact with hydrogen peroxide (30% v/v) at the same proportion (1/1 v/v), (b) after 4 hours, and (c) after 10 hours.
(a)
(b)
(c)


## 5. Recommendations for Nanosafety

No significant emission—compared to the background noise—was observed during the whole campaign. However, the possibility of exposure of humans to the emitted nanostructured material cannot be excluded in case of an accident, even though the workers involved in the production of nanoliposomes at UMINHO are protected by the use of nitrile gloves, cotton lab coat, and sometimes safety glasses. Considering this, the following recommendations are made here for future producers of devices in order to improve nanosecurity:(a)
All the operations of production of nanoliposomes should be carried out in each step under a closed conformity of a fume hood, particularly the extrusion and the purification of nanoliposomes activities.(b)
Two nitrile gloves per hand should be worn during all handling operations.(c)
During transport, a double containment of all materials should be implemented. Plastic bags with air tight zips can be used to wrap, for example.(d)
All the storages of nanomaterials should be clearly labeled.(e)
A proposition for a dilution-cracking based procedure for destroying the nanostructure of the nanoliposomes, thus simplifying the nanowaste management, has been made in the present study. The producers should use such a method to enhance the nanosafety with respect to their nanowaste.


## 6. Conclusion

During the campaign at the production site of the nanoliposome suspensions, an attentive study of the whole manufacturing process was carried out. Using the NF EN 689 standard, initial evaluations of possible sources of nanoliposome emissions were made. The quantification of the aerosol particles in the vicinity or within the breathing zone of these sources did not show any significant increase in their number concentration during various production activities. Their sampling showed the only presence of oil droplets or soot which are common particles of the urban aerosols produced by the human activity (traffic, heating). However, considering the nanowaste management and unanticipated accidents at the production site, simple and effective method and guidelines have also been proposed to ensure nanosafety.



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*Source: 931405-2015-10-08.xml*