# Rate and Timing Effects of Growth Regulating Herbicides Applications on Grain Sorghum (Sorghum bicolor) Growth and Yield

Authors: Thierry E. Besançon; Ranjit Riar; Ronnie W. Heiniger; Randy Weisz; Wesley J. Everman
Journal: Advances in Agriculture
 (2016)

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## Abstract

Dicamba and 2,4-D are among the most common and inexpensive herbicides used to control broadleaf weeds. However, different studies have pointed the risk of crop injury and grain sorghum yield reduction with postemergence applications of 2,4-D. No research data on grain sorghum response to 2,4-D or dicamba exists in the Southeastern United States. Consequently, a study was conducted to investigate crop growth and yield response to 2,4-D (100, 220, and 330 g acid equivalent ha−1) and dicamba (280 g acid equivalent ha−1) applied on 20 to 65 cm tall sorghum. Greater stunting resulted from 2,4-D applied at 330 g acid equivalent ha−1 or below 45 cm tall sorghum whereas lodging prevailed with 2,4-D at 330 g acid equivalent ha−1 and dicamba applied beyond 35 cm tall crop. Regardless of local environmental conditions, 2,4-D applied up to 35 cm tall did not negatively impact grain yield. There was a trend for yields to be somewhat lower when 2,4-D was applied on 45 or 55 cm tall sorghum whereas application on 65 cm tall sorghum systematically decreased yields. More caution should be taken with dicamba since yield reduction has been reported as early as applications made on 35 cm tall sorghum for a potentially dicamba sensitive cultivar.


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## Body

## 1. Introduction

Grain sorghum [Sorghum bicolor (L.) Moench], a major cereal crop grown in the United States, recently gained renewed interest in the southeastern region where it is used primarily as an animal feedstock. In 2012, 20,000 ha of grain sorghum was grown in North Carolina, an estimated 10-fold increase over 2011 [1]. Weed control remains a major challenge for sorghum producers worldwide as the percentage of grain sorghum yield lost due to weed competition exceeds that of most other grain crops [2]. Herbicide options for postemergence applications (POST) are more restricted in grain sorghum than for other major row crops because the sorghum worldwide market is not large enough to justify the development and registration of new herbicides [2]. Additionally, several products that used POST in sorghum have long rotation restrictions on their label that can limit their use in diverse cropping environments [3]. Among the POST herbicides available for controlling broadleaf weeds in grain sorghum, 2,4-D and dicamba are phenoxy and benzoic acid herbicides, respectively, that provide excellent control if applied while weed seedlings are young [4]. Furthermore, rotation to a non-glyphosate-resistant crop such as grain sorghum and the subsequent use of growth regulating herbicides is a recommended practice to improve control and prevent the spread of herbicide-resistance among several species of broadleaf weeds [5].While 2,4-D is a good option for weed control in grain sorghum, crop injury in the form of rolled leaves and brittle, spreading stems, and tillers may result from its application on grain sorghum [6]. Previous studies have shown that 2,4-D can cause a shortening of developing roots and shoots from sorghum seeds germinated on Petri dishes [7]. Sorghum sprayed with 2,4-D at 225 g acid equivalent (ae) ha−1 when plant height was 15 to 20 cm presented chromosomal aberrations within the pollen mother cells, mostly aneuploidy and polyploidy [8].Grain sorghum tolerance to 2,4-D may also differ among sorghum varieties. In a study comparing the competitiveness and herbicide tolerance of ten sorghum hybrids in Nebraska, 2,4-D applied at 1100 g ae ha−1 caused important sorghum injury by damaging roots, lodging, and abnormal growth independently of the cultivars. Most of the varieties recovered and yielded similarly to a weed-free check, except three that presented a significant yield decrease ranging from 12 to 27% [9]. Herbicide formulation also plays a role in the grain sorghum tolerance to 2,4-D. Low-volatile ester formulation provided better redroot pigweed control than amine salt but resulted in higher percentage of grain sorghum injury without impacting the yield [10].Recent studies have shown that grain sorghum productivity and lodging are sensitive to the rate of 2,4-D as well as phenological stage at which 2,4-D is applied. In a greenhouse experiment conducted in Brazil, Dan et al. [11] reported higher percentage of foliage injury with 2,4-D application made at the three-leaf stage rather than at the nine-leaf stage, regardless of 2,4-D rate ranging from 0 to 1,608 g ae ha−1. Yet, lodging was significantly more important when 2,4-D was applied at the nine-leaf stage compared to the three-leaf stage, with over 40% and < 10% lodged plants, respectively, for 2,4-D rate exceeding 840 g ae ha−1. Yield reduction ranging from 20 to 38% occurred for 2,4-D applied at the nine-leaf stage whereas loss did not exceed 25% for 1,608 g ae ha−1 2,4-D applied at the three-leaf stage [11]. Increasing rates of 2,4-D caused a linear reduction in plant height, biomass, and green dry matter accumulation as well as decreased yield, especially at rates above 1,000 g ae ha−1 and late applications [12]. In similar research, 2,4-D applied at the recommended rate (720 g ae ha−1) and 1.5x dosage at the five-leaf and eight-leaf stages has been reported to cause significant toxicity from 12 to 24% and reduce grain yield from 12 to 16% because of a decrease in the number of grains per panicle [13]. When compared to 2,4-D applied at 225 or 720 g ae ha−1, dicamba at 144 g ae ha−1 resulted in similar weed control but with less damage to sorghum and without reducing yield [14].Previous studies have shown dicamba injury on sensitive wheat (Triticum aestivum L.) varieties. Schroeder and Banks [15] reported crop injury and yield reduction when dicamba was applied at the midtillering stage on soft red winter wheat planted 10 or 21 days later than recommended. Schweizer et al. [16] found that sorghum yield was significantly reduced when a mixture of 280 g ae ha−1 of dicamba and 560 g ae ha−1 of 2,4-D was applied twice at the same rate in spring. Walker et al. [17] reported dicamba phytotoxicity on grain sorghum when leached into the soil but not when remaining on the soil surface. Dicamba applied at 280 or 560 g ae ha−1 one day before sowing significantly reduced sorghum seedling growth if 25 mm of irrigation was received within four days of sowing and yield if 144 mm of rainfall was received within 14 days of sowing.These results stress the need for assessing the potential damage of synthetic auxins applied POST over-the-top beyond the current sorghum height recommendation of 20 cm [18, 19]. Therefore, the objectives of this study were to determine the effect of 2,4-D and dicamba applied at different rates and phenological stages under different soil types and environmental conditions on grain sorghum growth, lodging, and yield.


## 2. Materials and Methods

Field experiments were conducted at the Upper Coastal Plain Research Station near Rocky Mount, NC, in 2012, 2013, and 2014, the Central Crops Research Station, Clayton, NC, in 2012 and 2013, and the Caswell Research Farm near Kinston, NC, in 2014 resulting in six year-by-location combinations (hereafter referred as to environments). All experiments were conducted under dryland conditions. Soils were a Roanoke loam (fine, mixed, semiactive, thermic Typic Endoaquults) with 2.3% organic matter and pH 6.4 in 2012 and 2013 and a Wickham sandy loam (fine-loamy, mixed, semiactive, thermic Typic Hapludults) associated with an AltaVista fine sandy loam (fine-loamy, mixed, semiactive, thermic Aquic Hapludults) with 1.3% organic matter and pH 5.7 in 2014 at Rocky Mount, Varina loamy sand (fine, kaolinitic, thermic Plinthic Paleudults) with 2.4% organic matter and pH 6 at Clayton, and Woodington loamy sand (coarse-loamy, siliceous, semiactive, thermic Typic Paleaquults) with 1.9% organic matter and pH 6.1 at Kinston.Sorghum hybrid DeKalb DKS 53-67 (Monsanto, Saint Louis, MO) was seeded at a rate of 296,000 seeds ha−1 at Rocky Mount and Kinston. At Clayton, sorghum cultivars Mycogen1G600 (Dow AgroSciences, Indianapolis, IN) and Pioneer 84P80 (DuPont, Johnston, IA) were seeded at a rate of 247,000 seeds ha−1 in 2012 and 2013, respectively. Fields were disk tilled before seeding at each environment and seeds were planted in 38 cm rows at a depth of 1.9 cm (see Table 1 for planting dates).S-Metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(1-methoxypropan-2-yl)acetamide] and atrazine [6-chloro-N2-ethyl-N4-(propan-2-yl)-1,3,5-triazine-2,4-diamine] herbicides (Bicep II Magnum® 15G, Syngenta Crop Protection, Greensboro, NC) were applied before emergence at 1412 and 1824 g active ingredient ha−1, respectively. Standard fertilization and insect management practices as recommended by the North Carolina Cooperative Extension Service were followed.Table 1
Planting and herbicide application dates.
Location
Year
Planting
Herbicide application
Harvest
PRE
POST
20 cm
35 cm
45 cm
55 cm
65 cm
Rocky Mount
2012
06/25
06/25
—
07/13
07/19
07/24
07/27
11/14
2013
06/14
06/14
—
07/22
07/25
08/1
08/5
11/3
2014
06/30
05/30
06/17
06/24
06/27
07/3
07/11
09/23
Clayton
2012
06/26
06/26
—
07/20
07/24
07/30
08/6
11/13
2013
06/28
06/26
—
08/7
08/12
08/20
08/23
11/14
Kinston
2014
05/27
05/27
06/14
06/23
06/29
07/7
07/14
10/7
Abbreviation: PRE, preemergence; POST, postemergence.The experimental design at each environment was a two-factorial arrangement in a randomized complete block with four replications. A weed-free control that did not receive POST application was added within each replication to serve as a comparison basis during the ratings. Plot size was 2.4 wide by 9.1 m long, with row spacing of 38 cm. Factors including synthetic auxins applied POST at different rates and timings. Herbicide treatments consisted of dimethylamine formulated 2,4-D [2-(2,4-dichlorophenoxy) acetic acid] (Weedar 64®, Nufarm, Burr Ridge, IL) at 100, 220, and 330 g ae ha−1 and dicamba [3,6-dichloro-2-methoxybenzoic acid] (Clarity®, BASF, Research Triangle Park, NC) at 280 g ae ha−1. POST treatments were applied when grain sorghum was approximately 35, 45, 55, or 65 cm tall. An additional application on 20 cm tall sorghum was added in 2014. No surfactant was added to POST treatments. In all years, the PRE and POST herbicides were applied in water with a CO2-pressurized backpack sprayer equipped with flat-fan nozzles XR1102 (TeeJet, Wheaton, IL) delivering 140 L ha−1 at 165 kPa (see Table 1 for applications dates).For each environment, grain sorghum height at boot stage as well as grain yield at harvest was recorded. Plots were machine harvested between mid-October and mid-November using a combine (Model Delta, Wintersteiger, Ried, Austria) specifically adapted for harvesting small plots and crop weights were adjusted to 14% moisture. In 2014, at Rocky Mount and Kinston, visual grain sorghum injury (chlorosis and stunting) and lodging were evaluated following synthetic auxins applications. Injury symptoms were only recorded in the form of stunting; no leaf chlorosis or necrosis was observed at both locations. These observations were based on a scale of 0 (no chlorosis, stunting, or lodging) to 100 (complete plant death or lodging) and recorded approximately 7, 21, and 35 days after application (DAA) as well as at harvest. Only injury in the form of stunting was observed at both locations and is therefore reported. Ratings taken before harvest did not show any stunting injury and are therefore not reported.Grain sorghum stunting and lodging data were analyzed as a two-way analysis of variance (ANOVA) using the PROC GLIMMIX procedure of SAS (version 9.4; SAS Institute Inc., Cary, NC). As stunting and lodging ratings were taken relatively to the weed-free control, this one was not included in the analysis. Data were transformed to the arcsine square root prior to ANOVA to stabilize the variances [20]. Because the transformation of the data did not change the separation of means, only nontransformed data are presented. The interaction between location and herbicide rate or application timing was not statistically significant for sorghum stunting and lodging. However, interaction between herbicide rate and application timing was significant (P
≤
0.05). Thus, data were analyzed and are presented by herbicide rates within each application timing or by application timings within each herbicide rate.Data for sorghum height at boot stage and grain yield at harvest were firstly subjected to a one-way ANOVA using the PROC GLIMMIX procedure of SAS (version 9.4; SAS Institute Inc., Cary, NC) and Fisher’s protected LSD test to separate treatments means (P
≤
0.05). The environment-by-treatments interaction was statistically significant for sorghum height at boot stage as well as grain yield at harvest; therefore, data are reported by environment. Subsequently, the dicamba treatment was dropped to separately analyze the effect of 2,4-D rates on grain sorghum yield and date were subjected to a one-way ANOVA using PROC GLIMMIX and Fisher’s protected LSD test to separate treatments means (P
≤
0.05). In the absence of environment-by-treatments interaction, 2,4-D treatments have been pooled over environments.


## 3. Results and Discussion

### 3.1. Crop Stunting and Lodging

Stunting was principally observed 7 and 21 DAA, with stunting becoming transient by 35 DAA. Sorghum stunting 7 and 21 DAA increased with higher rate of 2,4-D applied on 20 to 45 cm tall sorghum, ranging from 2 to 12% 7 DAA and from 0 to 7% 21 DAA with 2,4-D at 100 and 330 g ae ha−1, respectively (Table 2). No or minimal stunting (≤2%) regardless of 2,4-D rates was noted at 7 and 21 DAA with applications on sorghum taller than 45 cm. At 35 DAA, negligible stunting (≤2%) was only observed with 2,4-D applied at 330 g ae ha−1 on 20 or 32 cm tall sorghum. At 7 DAA and for any application timing, dicamba applied at 280 g ae ha−1 resulted in a higher percentage of stunting ranging from 3 to 17% than the highest rate of 2,4-D at 330 g ae ha−1. Similar result was observed 21 DAA for dicamba applied on 45 to 65 tall sorghum with stunting ranging from 1 to 4%. Regardless of time of application, limited stunting (≤2%) was noted 35 DAA for dicamba at 280 g ae ha−1.Table 2
Stunting from POST applications of 2,4-D and dicamba as affected by application timing in 2014 at Rocky Mount and Kinston, North Carolina.
Applicationtimingcm
Herbicideg ae ha−1
Stunting1,2
7 DAA
21 DAA
35 DAA
%
20
2,4-D 100
2c
0c
0a
2,4-D 220
7b
1c
0a
2,4-D 330
11
a
b
5a
1a
Dicamba
12
a
3b
0a
32
2,4-D 100
3d
0c
0b
2,4-D 220
8c
3b
0b
2,4-D 330
12b
7a
2a
Dicamba
17a
7a
2a
45
2,4-D 100
2c
0b
0b
2,4-D 220
2c
1b
0b
2,4-D 330
6b
2a
b
0b
Dicamba
10a
4a
2a
55
2,4-D 100
0b
0b
0a
2,4-D 220
0b
0b
0a
2,4-D 330
2a
0b
0a
Dicamba
5a
2a
1a
65
2,4-D 100
0b
0a
0a
2,4-D 220
0b
0a
0a
2,4-D 330
0b
0a
0a
Dicamba
3a
1a
0a
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.
2Abbreviation: DAA, days after application.2,4-D applied at 100 g ae ha−1 caused no or ≤3% stunting 7 DAA with greater injury recorded for 20 to 45 cm tall sorghum at time of application (Table 3). Increasing 2,4-D rate to 220 or 330 g ae ha−1 caused between 8% stunting and 12% stunting 7 DAA when sorghum was 20 to 35 cm tall at time of application. At 21 DAA, ≥5% stunting was only detected for 2,4-D at 330 g ae ha−1 applied on 20 to 35 cm tall sorghum. No or ≤3% stunting was observed 21 and 35 DAA with 2,4-D at 100 or 220 g ae ha−1 regardless of time of application or with 2,4-D at 330 g ae ha−1 applied beyond 35 cm sorghum height. Similar results have been reported by Dan et al. [11] who observed that 2,4-D applications during the early development stages of sorghum (up to six fully expanded leaves) resulted in the highest levels of toxicity when the rates of 2,4-D exceeded 210 g ae ha−1. Persistence of sorghum injury has also been previously documented up to 40 DAA with 16% injury when 2,4-D at 720 g ae ha−1 was applied at the five fully expanded leaves stage and 20% when applied at the eight fully expanded leaves stage [14].Table 3
Stunting from POST applications of 2,4-D and dicamba as affected by herbicide rate in 2014 at Rocky Mount and Kinston, North Carolina.
Herbicideg ae ha−1
Applicationtimingcm
Stunting1,2
7 DAA
21 DAA
35 DAA
%
2,4-D 100
20
2a
0a
0a
35
3a
0a
0a
45
2a
0a
0a
55
0b
0a
0a
65
0b
0a
0a
2,4-D 220
20
7a
1a
0a
35
8a
3a
0a
45
2b
1b
0a
55
0b
0b
0a
65
0b
0b
0a
2,4-D 330
20
11a
5a
1a
35
12a
7a
2a
45
6b
2b
0b
55
2c
0c
0b
65
0d
0c
0b
Dicamba
20
12b
3b
c
0b
35
17a
7a
2a
45
10b
4b
2a
55
6c
2c
d
1a
b
65
3c
1d
0b
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.
2DAA, days after application.Independently of the sorghum growth stage at which POST was applied, raising the 2,4-D rate from 100 to 330 g ae ha−1 increased the percentage of lodged plants by 2 to 4% 21 DAA and by 4 to 14% 35 DAA (Table 4). Application of 2,4-D beyond 35 cm sorghum height caused significant higher lodging at harvest for 330 g ae ha−1 compared to 100 and 220 g ae ha−1 with 6 to 7% and ≤2%, respectively. Depending on the time of application, dicamba applied at the labelled field rate for sorghum (280 g ae ha−1) caused lodging between 3 and 13% 21 DAA and 7 to 25% 35 DAA, similar results to those noted for 2,4-D applied at the highest rate (330 g ae ha−1).Table 4
Lodging from POST applications of 2,4-D and dicamba as affected by application timing in 2014 at Rocky Mount and Kinston, North Carolina.
Applicationtimingcm
Herbicideg ae ha−1
Lodging1,2
7 DAA
21 DAA
35 DAA
Harvest
%
20
2,4-D 100
0a
0b
2c
1a
2,4-D 220
0a
3a
b
3b
c
0a
2,4-D 330
1a
4a
6a
b
2a
Dicamba
1a
3a
b
7a
2a
35
2,4-D 100
0b
2b
3b
2a
2,4-D 220
0b
3a
b
5b
1a
2,4-D 330
0b
6a
13a
4a
Dicamba
2a
4a
15a
3a
45
2,4-D 100
0b
3c
3b
1b
2,4-D 220
0b
5b
c
6b
1b
2,4-D 330
2a
b
6a
b
15a
6a
Dicamba
3a
8a
17a
5a
55
2,4-D 100
0c
2c
7b
1b
2,4-D 220
2b
c
4c
10b
2b
2,4-D 330
4a
b
7b
21a
6a
Dicamba
6a
13a
25a
8a
65
2,4-D 100
0c
5b
8b
2c
2,4-D 220
2b
c
5b
10b
2c
2,4-D 330
5a
b
9a
b
19a
7b
Dicamba
6a
12a
21a
13a
1Means within a column followed by the same letter are not different according to Fisher’s protected LSD test at P
≤
0.05.
2DAA, days after application.Our data also show that grain sorghum is more sensitive to lodging resulting from growth regulating herbicides applied at later sorghum stages (Table5). None or minimal lodging (≤10%) was observed at 7, 21, and 35 DAA or at harvest for 2,4-D applied at 100 or 220 g ae ha−1. Dicamba at 280 g ae ha−1 or 2,4-D at 330 g ae ha−1 caused 15 to 25% lodging when applied on sorghum taller than 35 cm. Persistence of lodging for dicamba or the highest rate of 2,4-D applied beyond 55 cm tall sorghum was noted up to harvest with 6 to 13% lodging compared to 2% when application was made on 20 cm tall sorghum.Table 5
Lodging from POST applications of 2,4-D and dicamba as affected by herbicide rate in 2014 at Rocky Mount and Kinston, North Carolina.
Herbicideg ae ha−1
Applicationtimingcm
Lodging1,2
7 DAA
21 DAA
35 DAA
Harvest
%
2,4-D 100
20
0a
0b
2b
0a
35
0a
2a
b
3b
2a
45
0a
3a
b
3b
0a
55
0a
2a
b
7a
1a
65
0a
5a
8a
2a
2,4-D 220
20
0b
3a
3b
0a
35
0b
4a
5b
1a
45
0b
5a
6b
1a
55
1a
b
4a
10a
2a
65
2a
5a
10a
2a
2,4-D 330
20
1c
4b
6d
2b
35
0c
6a
b
13c
4a
b
45
2b
c
6a
b
15b
c
6a
55
4a
b
7a
b
21a
6a
65
5a
9a
19a
b
7a
Dicamba
20
1b
3c
7d
2c
35
2b
4c
15c
3b
c
45
3a
b
8b
17b
c
5b
c
55
6a
13a
25a
8b
65
6a
12a
b
21a
b
13a
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.
2DAA, days after application.Dan et al. [11] reported an increased percentage of lodged plants when 2,4-D was applied at a rate exceeding 420 g ae ha−1 on grain sorghum plants with at least nine expanded leaves with up to 36% at 840 g ae ha−1 and 50% at 1608 g ae ha−1. In vitro application of 2,4-D has been shown to induce a shortening of sorghum roots accompanied by uncontrolled growth of adventitious roots [7, 21]. Root systems from susceptible species in nutrient solution containing 0.5 ppm 2,4-D also exhibited increased cellulase activity [22]. Increasing rates of 2,4-D from 280 to 1120 g ae ha−1, applied on grain sorghum at the five-leaf stage, caused fasciation of the brace roots and delayed development of the feeder roots without affecting the yield if sufficient moisture was available [23]. As a consequence of the weakened root systems, grain sorghum plants sprayed with 2,4-D are more prone to lodging as observed in our study. Lodging evaluation before harvest suggests that grain sorghum may partially recover from injury observed earlier in the season. However, greater lodging damages persisted when dicamba or 2,4-D at the highest rate was applied on sorghum taller than 35 cm.


### 3.2. Crop Height at Boot Stage

Consistent sorghum height increase at boot stage as compared to the weed-free control that did not receive POST application of growth regulating was observed in three of six environments (Table6). At Rocky Mount and Clayton in 2012 as well as at Kinston in 2014, 2,4-D applied on 35 to 55 cm tall sorghum caused sorghum height to increase by 9% on average for a 220 g ae ha−1 rate and by 10% on average for a 330 g ae ha−1 rate compared to the control. Sorghum height increased by 8% at Rocky Mount in 2012 and by 11% at Kinston in 2014 compared to the control when 2,4-D was applied at 100 g ae ha−1 on 45 or 55 cm tall plants. Regardless of herbicide rates, application of 2,4-D on 65 cm tall sorghum did not cause plants to grow taller than in the control. Similarly, dicamba applied at 280 g ae ha−1 had little or no effect on crop height, only a slight increase by 6% compared to the control being noted at Rocky Mount in 2012 and Kinston in 2014 when dicamba was applied on 45 or 55 cm tall sorghum. Other studies have reported nonsignificant decrease in plant height with 2,4-D applied at 335, 670, or 1005 g ae ha−1 and on three- to six-leaf stage grain sorghum [12] whereas significant reduction occurred at the same 2,4-D rates on three-leaf stage pearl millet [Pennisetum americanum (L.) Leeke] [24]. These contrasting results may be due to differential sensitivity to 2,4-D, which is associated with genetic variations of the different sorghum cultivars.Table 6
Sorghum height at boot stage as affected by growth regulating herbicide rate and application timing in North Carolina1.
Herbicide
Rateg ae ha−1
Timingcm
Rocky Mount
Clayton
Kinston
2012
2013
2014
2012
2013
2014
cm
2,4-D
100
35
75a
b
101a
87a
b
58b–d
84b
c
86e–h
220
35
77a
95a
b
86a
b
59b
c
85b
c
91a–d
330
35
78a
103a
85a
b
59b
c
85b
c
91a–d
Dicamba
280
35
70b
88b
89a
58b–d
85b
c
90b–e
2,4-D
100
45
76a
94a
b
83b
58b–d
89a–c
95a
220
45
78a
94a
b
84b
62a–c
83c
93a
b
330
45
77a
101a
86a
b
63a
85b
c
93a
b
Dicamba
280
45
78a
94a
b
87a
b
53e
85b
c
91a–d
2,4-D
100
55
76a
99a
86a
b
62a
b
85b
c
91a–d
220
55
78a
100a
85a
b
61a–c
93a
b
88c–f
330
55
77a
104a
87a
b
59a–d
88a–c
88c–f
Dicamba
280
55
76a
95a
b
85a
b
57c–e
86b
c
88c–f
2,4-D
100
65
74a
b
100a
88a
b
56d–e
95a
b
81h
220
65
75a
b
96a
b
87a
b
58a–d
90a–c
83g
h
330
65
75a
b
102a
86a
b
57c–e
93a–c
86e–h
Dicamba
280
65
74a
b
95a
b
86a
b
57b–e
97a
83g
h
Weed-free control
71b
101a
85a
b
55d
e
86a–c
83g
h
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.


### 3.3. Grain Yield at Harvest

As compared to the weed-free control, lower grain yield was noted in four of six environments when dicamba at 280 g ae ha−1 was applied on 55 and 65 cm tall sorghum (Table 7). At Clayton and Rocky Mount in 2012 as well as at Kinston and Rocky Mount in 2014, dicamba applied on 55 cm tall sorghum resulted in significant yield reduction averaging 1.3 Mg ha−1. Similar effect was observed on 65 cm tall sorghum at Rocky Mount in 2012 and 2013, Clayton in 2012, and Kinston in 2014 with significant yield reduction averaging 1.5 Mg ha−1. Earlier application on 35 or 45 cm tall sorghum did not cause grain yield loss, except at Clayton in 2012 with reduction by 1.7 and 1.2 Mg ha−1, respectively. Systematic yield reduction at Clayton in 2012 with dicamba applied at any sorghum growth stage may be due to specific cultivar sensitivity to dicamba as Mycogen1G600 was used for this environment whereas DeKalb DKS 53-67 and Pioneer 84P80 were planted in all the other environments. If cultivar sensitivity has not been reported for sorghum, previous studies have shown dicamba injury on sensitive wheat varieties [15, 25]. Rinella et al. [26] have shown that dicamba applied at 140 of 280 g ae ha−1on F2 to F10 “Wakefield” winter wheat cultivar caused 30 to 100% underdeveloped seeds that were too small to be harvested. Awad et al. [27] have indicated that there was rapid and extensive metabolism of absorbed dicamba by sorghum with 32% of the 14C in shoots above treated leaves being metabolized by sorghum 7 days after treatment. Therefore, it is possible that Mycogen1G600 does not metabolize dicamba as rapidly as other cultivars, and underdeveloped seeds may result from herbicide remaining in the plant at the time of gamete formation, anthesis, and seed growth and development.Table 7
Grain yield as affected by growth regulating herbicide rate and application timing in North Carolina1.
Herbicide
Rateg ae ha−1
Timingcm
Rocky Mount
Clayton
Kinston
2012
2013
2014
2012
2013
2014
Mg ha−1
2,4-D
100
35
8.0a–c
4.5a–c
6.6a
2.2a–c
1.5a
b
5.4ab
220
35
8.5a
4.2bc
6.0a–c
2.4a
b
1.6a
6.1a
330
35
7.7b–d
5.2a
b
6.2a
b
2.6a
1.3a
b
6.1a
Dicamba
280
35
8.4a
b
4.3a–c
6.4a
b
0.6fg
1.0a–c
5.7ab
2,4-D
100
45
8.0a–c
4.5a–c
4.4c–e
2.3a
b
1.2a
b
6.0a
220
45
8.1a
b
4.5a–c
3.6e
2.2a
b
1.0a–c
5.6ab
330
45
8.3a
b
5.5a
5.5b–d
2.4a
b
1.5a
b
4.4cd
Dicamba
280
45
8.3a
b
4.5a–c
4.6c–e
1.1d–f
1.5a
4.5b–d
2,4-D
100
55
7.9a–c
5.1a
b
4.3de
2.0a–c
1.1a–c
5.1a–c
220
55
7.9a–c
4.7a–c
5.1b–e
1.8b–d
0.4c
5.1a–c
330
55
7.8b–d
5.2a
b
5.0b–e
2.1a–c
0.6bc
5.0a–c
Dicamba
280
55
7.3cd
4.2a–c
3.6e
0.2a
1.4a
b
4.7bc
2,4-D
100
65
7.3cd
4.2b
c
5.7a–d
2.2a
b
1.3a
b
5.1a–c
220
65
7.6b–d
4.5a–c
4.4c–e
1.5c–e
0.7bc
5.0a–c
330
65
7.6b–d
4.6a–c
4.4c–e
1.9a–c
1.0a–c
4.3cd
Dicamba
280
65
7.0d
3.5c
5.4a–d
0.9e–g
1.4a
b
3.5d
Weed-free control
8.2a
b
4.9a
b
4.8b–e
2.3a
b
1.3a
b
5.8a
b
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.In the absence of environment-by-treatment interaction after dicamba was removed from the ANOVA analysis, yield data were pooled over environments and are presented in Figure1. 2,4-D applied at 100, 220, or 330 g ae ha−1 on 35 cm tall sorghum did not cause significant yield reduction compared to the control. Regardless of herbicide rate, grain yield was not statistically different from the control when 2,4-D application occurred on 45 or 55 cm tall sorghum. However, compared to the average yield for 2,4-D applied on 35 cm tall sorghum, significant reduction by 12% was noted with 2,4-D at 220 g ae ha−1 applied at 45 cm or at any rate when applied on 55 cm tall sorghum. At the latest stage of application (65 cm), 2,4-D at 100 g ae ha−1 did not significantly reduce yield compared to the control but decreased it by 10% compared to the average yield for 2,4-D applied on 35 cm tall sorghum. Increasing 2,4-D rate to 220 or 330 g ae ha−1 at the 65 cm stage caused significant yield reduction by 13% compared to the control or by 18% compared to the average yield for 2,4-D applied on 35 cm tall sorghum.Figure 1
Effect of sorghum height at application time on grain yield for three rates of 2,4-D in North Carolina. Data were pooled over environments and means followed by the same letters are not different according to Fisher’s protected LSD test (P
≤
0.05).Reduction in grain yield ranging from 12 to 16% was also noted for 2,4-D was applied on V5 or V8 sorghum and at 720 or 1080 g ae ha−1 [13]. Yield reduction was caused by a decreased number of grains per panicle.At 21 and 35 DAA, there was a statistically significant correlation (P
≤
0.01) between lodging injury and grain yield (Table 8). While the associated R-values were low, this response suggests that the complete recovery from lodging observed by harvest did not compensate for the negative impact that lodging may have had before sorghum pollination.Table 8
Pearson correlation coefficients between crop stunting or lodging and sorghum yield at Rocky Mount and Kinston, North Carolina, in 20141.
Injury
Rating
7 DAA
21 DAA
35 DAA
Stunting
0.10 (P
=
0.21)
0.03 (P
=
0.73)
−0.01 (P
=
0.88)
Lodging
−0.20 (P
=
0.01)
−0.28 (P
≤
0.01)
−0.27 (P
≤
0.01)
DAA, days after treatment.


## 3.1. Crop Stunting and Lodging

Stunting was principally observed 7 and 21 DAA, with stunting becoming transient by 35 DAA. Sorghum stunting 7 and 21 DAA increased with higher rate of 2,4-D applied on 20 to 45 cm tall sorghum, ranging from 2 to 12% 7 DAA and from 0 to 7% 21 DAA with 2,4-D at 100 and 330 g ae ha−1, respectively (Table 2). No or minimal stunting (≤2%) regardless of 2,4-D rates was noted at 7 and 21 DAA with applications on sorghum taller than 45 cm. At 35 DAA, negligible stunting (≤2%) was only observed with 2,4-D applied at 330 g ae ha−1 on 20 or 32 cm tall sorghum. At 7 DAA and for any application timing, dicamba applied at 280 g ae ha−1 resulted in a higher percentage of stunting ranging from 3 to 17% than the highest rate of 2,4-D at 330 g ae ha−1. Similar result was observed 21 DAA for dicamba applied on 45 to 65 tall sorghum with stunting ranging from 1 to 4%. Regardless of time of application, limited stunting (≤2%) was noted 35 DAA for dicamba at 280 g ae ha−1.Table 2
Stunting from POST applications of 2,4-D and dicamba as affected by application timing in 2014 at Rocky Mount and Kinston, North Carolina.
Applicationtimingcm
Herbicideg ae ha−1
Stunting1,2
7 DAA
21 DAA
35 DAA
%
20
2,4-D 100
2c
0c
0a
2,4-D 220
7b
1c
0a
2,4-D 330
11
a
b
5a
1a
Dicamba
12
a
3b
0a
32
2,4-D 100
3d
0c
0b
2,4-D 220
8c
3b
0b
2,4-D 330
12b
7a
2a
Dicamba
17a
7a
2a
45
2,4-D 100
2c
0b
0b
2,4-D 220
2c
1b
0b
2,4-D 330
6b
2a
b
0b
Dicamba
10a
4a
2a
55
2,4-D 100
0b
0b
0a
2,4-D 220
0b
0b
0a
2,4-D 330
2a
0b
0a
Dicamba
5a
2a
1a
65
2,4-D 100
0b
0a
0a
2,4-D 220
0b
0a
0a
2,4-D 330
0b
0a
0a
Dicamba
3a
1a
0a
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.
2Abbreviation: DAA, days after application.2,4-D applied at 100 g ae ha−1 caused no or ≤3% stunting 7 DAA with greater injury recorded for 20 to 45 cm tall sorghum at time of application (Table 3). Increasing 2,4-D rate to 220 or 330 g ae ha−1 caused between 8% stunting and 12% stunting 7 DAA when sorghum was 20 to 35 cm tall at time of application. At 21 DAA, ≥5% stunting was only detected for 2,4-D at 330 g ae ha−1 applied on 20 to 35 cm tall sorghum. No or ≤3% stunting was observed 21 and 35 DAA with 2,4-D at 100 or 220 g ae ha−1 regardless of time of application or with 2,4-D at 330 g ae ha−1 applied beyond 35 cm sorghum height. Similar results have been reported by Dan et al. [11] who observed that 2,4-D applications during the early development stages of sorghum (up to six fully expanded leaves) resulted in the highest levels of toxicity when the rates of 2,4-D exceeded 210 g ae ha−1. Persistence of sorghum injury has also been previously documented up to 40 DAA with 16% injury when 2,4-D at 720 g ae ha−1 was applied at the five fully expanded leaves stage and 20% when applied at the eight fully expanded leaves stage [14].Table 3
Stunting from POST applications of 2,4-D and dicamba as affected by herbicide rate in 2014 at Rocky Mount and Kinston, North Carolina.
Herbicideg ae ha−1
Applicationtimingcm
Stunting1,2
7 DAA
21 DAA
35 DAA
%
2,4-D 100
20
2a
0a
0a
35
3a
0a
0a
45
2a
0a
0a
55
0b
0a
0a
65
0b
0a
0a
2,4-D 220
20
7a
1a
0a
35
8a
3a
0a
45
2b
1b
0a
55
0b
0b
0a
65
0b
0b
0a
2,4-D 330
20
11a
5a
1a
35
12a
7a
2a
45
6b
2b
0b
55
2c
0c
0b
65
0d
0c
0b
Dicamba
20
12b
3b
c
0b
35
17a
7a
2a
45
10b
4b
2a
55
6c
2c
d
1a
b
65
3c
1d
0b
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.
2DAA, days after application.Independently of the sorghum growth stage at which POST was applied, raising the 2,4-D rate from 100 to 330 g ae ha−1 increased the percentage of lodged plants by 2 to 4% 21 DAA and by 4 to 14% 35 DAA (Table 4). Application of 2,4-D beyond 35 cm sorghum height caused significant higher lodging at harvest for 330 g ae ha−1 compared to 100 and 220 g ae ha−1 with 6 to 7% and ≤2%, respectively. Depending on the time of application, dicamba applied at the labelled field rate for sorghum (280 g ae ha−1) caused lodging between 3 and 13% 21 DAA and 7 to 25% 35 DAA, similar results to those noted for 2,4-D applied at the highest rate (330 g ae ha−1).Table 4
Lodging from POST applications of 2,4-D and dicamba as affected by application timing in 2014 at Rocky Mount and Kinston, North Carolina.
Applicationtimingcm
Herbicideg ae ha−1
Lodging1,2
7 DAA
21 DAA
35 DAA
Harvest
%
20
2,4-D 100
0a
0b
2c
1a
2,4-D 220
0a
3a
b
3b
c
0a
2,4-D 330
1a
4a
6a
b
2a
Dicamba
1a
3a
b
7a
2a
35
2,4-D 100
0b
2b
3b
2a
2,4-D 220
0b
3a
b
5b
1a
2,4-D 330
0b
6a
13a
4a
Dicamba
2a
4a
15a
3a
45
2,4-D 100
0b
3c
3b
1b
2,4-D 220
0b
5b
c
6b
1b
2,4-D 330
2a
b
6a
b
15a
6a
Dicamba
3a
8a
17a
5a
55
2,4-D 100
0c
2c
7b
1b
2,4-D 220
2b
c
4c
10b
2b
2,4-D 330
4a
b
7b
21a
6a
Dicamba
6a
13a
25a
8a
65
2,4-D 100
0c
5b
8b
2c
2,4-D 220
2b
c
5b
10b
2c
2,4-D 330
5a
b
9a
b
19a
7b
Dicamba
6a
12a
21a
13a
1Means within a column followed by the same letter are not different according to Fisher’s protected LSD test at P
≤
0.05.
2DAA, days after application.Our data also show that grain sorghum is more sensitive to lodging resulting from growth regulating herbicides applied at later sorghum stages (Table5). None or minimal lodging (≤10%) was observed at 7, 21, and 35 DAA or at harvest for 2,4-D applied at 100 or 220 g ae ha−1. Dicamba at 280 g ae ha−1 or 2,4-D at 330 g ae ha−1 caused 15 to 25% lodging when applied on sorghum taller than 35 cm. Persistence of lodging for dicamba or the highest rate of 2,4-D applied beyond 55 cm tall sorghum was noted up to harvest with 6 to 13% lodging compared to 2% when application was made on 20 cm tall sorghum.Table 5
Lodging from POST applications of 2,4-D and dicamba as affected by herbicide rate in 2014 at Rocky Mount and Kinston, North Carolina.
Herbicideg ae ha−1
Applicationtimingcm
Lodging1,2
7 DAA
21 DAA
35 DAA
Harvest
%
2,4-D 100
20
0a
0b
2b
0a
35
0a
2a
b
3b
2a
45
0a
3a
b
3b
0a
55
0a
2a
b
7a
1a
65
0a
5a
8a
2a
2,4-D 220
20
0b
3a
3b
0a
35
0b
4a
5b
1a
45
0b
5a
6b
1a
55
1a
b
4a
10a
2a
65
2a
5a
10a
2a
2,4-D 330
20
1c
4b
6d
2b
35
0c
6a
b
13c
4a
b
45
2b
c
6a
b
15b
c
6a
55
4a
b
7a
b
21a
6a
65
5a
9a
19a
b
7a
Dicamba
20
1b
3c
7d
2c
35
2b
4c
15c
3b
c
45
3a
b
8b
17b
c
5b
c
55
6a
13a
25a
8b
65
6a
12a
b
21a
b
13a
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.
2DAA, days after application.Dan et al. [11] reported an increased percentage of lodged plants when 2,4-D was applied at a rate exceeding 420 g ae ha−1 on grain sorghum plants with at least nine expanded leaves with up to 36% at 840 g ae ha−1 and 50% at 1608 g ae ha−1. In vitro application of 2,4-D has been shown to induce a shortening of sorghum roots accompanied by uncontrolled growth of adventitious roots [7, 21]. Root systems from susceptible species in nutrient solution containing 0.5 ppm 2,4-D also exhibited increased cellulase activity [22]. Increasing rates of 2,4-D from 280 to 1120 g ae ha−1, applied on grain sorghum at the five-leaf stage, caused fasciation of the brace roots and delayed development of the feeder roots without affecting the yield if sufficient moisture was available [23]. As a consequence of the weakened root systems, grain sorghum plants sprayed with 2,4-D are more prone to lodging as observed in our study. Lodging evaluation before harvest suggests that grain sorghum may partially recover from injury observed earlier in the season. However, greater lodging damages persisted when dicamba or 2,4-D at the highest rate was applied on sorghum taller than 35 cm.


## 3.2. Crop Height at Boot Stage

Consistent sorghum height increase at boot stage as compared to the weed-free control that did not receive POST application of growth regulating was observed in three of six environments (Table6). At Rocky Mount and Clayton in 2012 as well as at Kinston in 2014, 2,4-D applied on 35 to 55 cm tall sorghum caused sorghum height to increase by 9% on average for a 220 g ae ha−1 rate and by 10% on average for a 330 g ae ha−1 rate compared to the control. Sorghum height increased by 8% at Rocky Mount in 2012 and by 11% at Kinston in 2014 compared to the control when 2,4-D was applied at 100 g ae ha−1 on 45 or 55 cm tall plants. Regardless of herbicide rates, application of 2,4-D on 65 cm tall sorghum did not cause plants to grow taller than in the control. Similarly, dicamba applied at 280 g ae ha−1 had little or no effect on crop height, only a slight increase by 6% compared to the control being noted at Rocky Mount in 2012 and Kinston in 2014 when dicamba was applied on 45 or 55 cm tall sorghum. Other studies have reported nonsignificant decrease in plant height with 2,4-D applied at 335, 670, or 1005 g ae ha−1 and on three- to six-leaf stage grain sorghum [12] whereas significant reduction occurred at the same 2,4-D rates on three-leaf stage pearl millet [Pennisetum americanum (L.) Leeke] [24]. These contrasting results may be due to differential sensitivity to 2,4-D, which is associated with genetic variations of the different sorghum cultivars.Table 6
Sorghum height at boot stage as affected by growth regulating herbicide rate and application timing in North Carolina1.
Herbicide
Rateg ae ha−1
Timingcm
Rocky Mount
Clayton
Kinston
2012
2013
2014
2012
2013
2014
cm
2,4-D
100
35
75a
b
101a
87a
b
58b–d
84b
c
86e–h
220
35
77a
95a
b
86a
b
59b
c
85b
c
91a–d
330
35
78a
103a
85a
b
59b
c
85b
c
91a–d
Dicamba
280
35
70b
88b
89a
58b–d
85b
c
90b–e
2,4-D
100
45
76a
94a
b
83b
58b–d
89a–c
95a
220
45
78a
94a
b
84b
62a–c
83c
93a
b
330
45
77a
101a
86a
b
63a
85b
c
93a
b
Dicamba
280
45
78a
94a
b
87a
b
53e
85b
c
91a–d
2,4-D
100
55
76a
99a
86a
b
62a
b
85b
c
91a–d
220
55
78a
100a
85a
b
61a–c
93a
b
88c–f
330
55
77a
104a
87a
b
59a–d
88a–c
88c–f
Dicamba
280
55
76a
95a
b
85a
b
57c–e
86b
c
88c–f
2,4-D
100
65
74a
b
100a
88a
b
56d–e
95a
b
81h
220
65
75a
b
96a
b
87a
b
58a–d
90a–c
83g
h
330
65
75a
b
102a
86a
b
57c–e
93a–c
86e–h
Dicamba
280
65
74a
b
95a
b
86a
b
57b–e
97a
83g
h
Weed-free control
71b
101a
85a
b
55d
e
86a–c
83g
h
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.


## 3.3. Grain Yield at Harvest

As compared to the weed-free control, lower grain yield was noted in four of six environments when dicamba at 280 g ae ha−1 was applied on 55 and 65 cm tall sorghum (Table 7). At Clayton and Rocky Mount in 2012 as well as at Kinston and Rocky Mount in 2014, dicamba applied on 55 cm tall sorghum resulted in significant yield reduction averaging 1.3 Mg ha−1. Similar effect was observed on 65 cm tall sorghum at Rocky Mount in 2012 and 2013, Clayton in 2012, and Kinston in 2014 with significant yield reduction averaging 1.5 Mg ha−1. Earlier application on 35 or 45 cm tall sorghum did not cause grain yield loss, except at Clayton in 2012 with reduction by 1.7 and 1.2 Mg ha−1, respectively. Systematic yield reduction at Clayton in 2012 with dicamba applied at any sorghum growth stage may be due to specific cultivar sensitivity to dicamba as Mycogen1G600 was used for this environment whereas DeKalb DKS 53-67 and Pioneer 84P80 were planted in all the other environments. If cultivar sensitivity has not been reported for sorghum, previous studies have shown dicamba injury on sensitive wheat varieties [15, 25]. Rinella et al. [26] have shown that dicamba applied at 140 of 280 g ae ha−1on F2 to F10 “Wakefield” winter wheat cultivar caused 30 to 100% underdeveloped seeds that were too small to be harvested. Awad et al. [27] have indicated that there was rapid and extensive metabolism of absorbed dicamba by sorghum with 32% of the 14C in shoots above treated leaves being metabolized by sorghum 7 days after treatment. Therefore, it is possible that Mycogen1G600 does not metabolize dicamba as rapidly as other cultivars, and underdeveloped seeds may result from herbicide remaining in the plant at the time of gamete formation, anthesis, and seed growth and development.Table 7
Grain yield as affected by growth regulating herbicide rate and application timing in North Carolina1.
Herbicide
Rateg ae ha−1
Timingcm
Rocky Mount
Clayton
Kinston
2012
2013
2014
2012
2013
2014
Mg ha−1
2,4-D
100
35
8.0a–c
4.5a–c
6.6a
2.2a–c
1.5a
b
5.4ab
220
35
8.5a
4.2bc
6.0a–c
2.4a
b
1.6a
6.1a
330
35
7.7b–d
5.2a
b
6.2a
b
2.6a
1.3a
b
6.1a
Dicamba
280
35
8.4a
b
4.3a–c
6.4a
b
0.6fg
1.0a–c
5.7ab
2,4-D
100
45
8.0a–c
4.5a–c
4.4c–e
2.3a
b
1.2a
b
6.0a
220
45
8.1a
b
4.5a–c
3.6e
2.2a
b
1.0a–c
5.6ab
330
45
8.3a
b
5.5a
5.5b–d
2.4a
b
1.5a
b
4.4cd
Dicamba
280
45
8.3a
b
4.5a–c
4.6c–e
1.1d–f
1.5a
4.5b–d
2,4-D
100
55
7.9a–c
5.1a
b
4.3de
2.0a–c
1.1a–c
5.1a–c
220
55
7.9a–c
4.7a–c
5.1b–e
1.8b–d
0.4c
5.1a–c
330
55
7.8b–d
5.2a
b
5.0b–e
2.1a–c
0.6bc
5.0a–c
Dicamba
280
55
7.3cd
4.2a–c
3.6e
0.2a
1.4a
b
4.7bc
2,4-D
100
65
7.3cd
4.2b
c
5.7a–d
2.2a
b
1.3a
b
5.1a–c
220
65
7.6b–d
4.5a–c
4.4c–e
1.5c–e
0.7bc
5.0a–c
330
65
7.6b–d
4.6a–c
4.4c–e
1.9a–c
1.0a–c
4.3cd
Dicamba
280
65
7.0d
3.5c
5.4a–d
0.9e–g
1.4a
b
3.5d
Weed-free control
8.2a
b
4.9a
b
4.8b–e
2.3a
b
1.3a
b
5.8a
b
1Means within a column followed by the same letters are not different according to Fisher’s protected LSD test at P
≤
0.05.In the absence of environment-by-treatment interaction after dicamba was removed from the ANOVA analysis, yield data were pooled over environments and are presented in Figure1. 2,4-D applied at 100, 220, or 330 g ae ha−1 on 35 cm tall sorghum did not cause significant yield reduction compared to the control. Regardless of herbicide rate, grain yield was not statistically different from the control when 2,4-D application occurred on 45 or 55 cm tall sorghum. However, compared to the average yield for 2,4-D applied on 35 cm tall sorghum, significant reduction by 12% was noted with 2,4-D at 220 g ae ha−1 applied at 45 cm or at any rate when applied on 55 cm tall sorghum. At the latest stage of application (65 cm), 2,4-D at 100 g ae ha−1 did not significantly reduce yield compared to the control but decreased it by 10% compared to the average yield for 2,4-D applied on 35 cm tall sorghum. Increasing 2,4-D rate to 220 or 330 g ae ha−1 at the 65 cm stage caused significant yield reduction by 13% compared to the control or by 18% compared to the average yield for 2,4-D applied on 35 cm tall sorghum.Figure 1
Effect of sorghum height at application time on grain yield for three rates of 2,4-D in North Carolina. Data were pooled over environments and means followed by the same letters are not different according to Fisher’s protected LSD test (P
≤
0.05).Reduction in grain yield ranging from 12 to 16% was also noted for 2,4-D was applied on V5 or V8 sorghum and at 720 or 1080 g ae ha−1 [13]. Yield reduction was caused by a decreased number of grains per panicle.At 21 and 35 DAA, there was a statistically significant correlation (P
≤
0.01) between lodging injury and grain yield (Table 8). While the associated R-values were low, this response suggests that the complete recovery from lodging observed by harvest did not compensate for the negative impact that lodging may have had before sorghum pollination.Table 8
Pearson correlation coefficients between crop stunting or lodging and sorghum yield at Rocky Mount and Kinston, North Carolina, in 20141.
Injury
Rating
7 DAA
21 DAA
35 DAA
Stunting
0.10 (P
=
0.21)
0.03 (P
=
0.73)
−0.01 (P
=
0.88)
Lodging
−0.20 (P
=
0.01)
−0.28 (P
≤
0.01)
−0.27 (P
≤
0.01)
DAA, days after treatment.


## 4. Conclusion

This research demonstrated that POST application of growth regulating herbicides beyond the current recommended sorghum height caused increasing lodging, greater injury resulting from application of 2,4-D at 330 g ae ha−1 or dicamba at 280 g ae ha−1. In any situation, application of dicamba at the current recommended rate of 280 g ae ha−1 resulted in higher stunting damage than 2,4-D in the days following application. However, unlike lodging, stunting symptoms were not correlated with yield reduction. Since growth regulating herbicide may also affect sorghum root system, damage occurring before pollination may not be compensated early enough to prevent yield reduction. Regardless of local environmental conditions, 2,4-D which applied up to 35 cm tall sorghum could improve broadleaf weed control in grain sorghum systems without negatively impacting grain yield. Applying 2,4-D on 45 or 55 cm tall sorghum had similar yield impact at each rate. There was a trend for yields to be somewhat lower, but it was only statistically significant at the 220 g ae ha−1 rate. However, application of 2,4-D on 65 cm tall sorghum systematically resulted in significant lower yields. More caution should be taken with dicamba applied at 280 g ae ha−1 since yield reduction has been reported as early as applications made on 35 cm tall sorghum for a sorghum cultivar potentially sensitive to dicamba. Further research should be conducted to address potential differences in sensitivity to dicamba and 2,4-D among sorghum cultivars that are currently commercially available. With regard to the negative impact of dicamba applied over-the-top on sorghum yield observed in our study, concerns may arise from the recent introduction of dicamba-resistant cotton or soybean that could result in off-target movement of dicamba and potentially increase lodging and reduce yield in neighboring sorghum fields. Therefore, investigations should also be directed to evaluate the effects of dicamba or 2,4-D drift on grain sorghum development and commercial yield.



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*Source: 9302507-2016-09-15.xml*