# Portulaca oleracea L.: A Review of Phytochemistry and Pharmacological Effects

Authors: Yan-Xi Zhou; Hai-Liang Xin; Khalid Rahman; Su-Juan Wang; Cheng Peng; Hong Zhang
Journal: BioMed Research International
 (2015)

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## Abstract

Portulaca oleracea L., belonging to the Portulacaceae family, is commonly known
as purslane in English and Ma-Chi-Xian in Chinese. It is a warm-climate, herbaceous succulent
annual plant with a cosmopolitan distribution. It is eaten extensively as a potherb and added in
soups and salads around the Mediterranean and tropical Asian countries and has been used as a
folk medicine in many countries. Diverse compounds have been isolated from Portulaca oleracea, such
as flavonoids, alkaloids, polysaccharides, fatty acids, terpenoids, sterols, proteins vitamins and minerals.
Portulaca oleracea possesses a wide spectrum of pharmacological properties such as
neuroprotective, antimicrobial, antidiabetic, antioxidant, anti-inflammatory, antiulcerogenic, and anticancer
activities. However, few molecular mechanisms of action are known.
This review provides a summary of phytochemistry and pharmacological effects of this plant.


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## Body

## 1. Introduction

Portulaca oleracea L. is a warm-climate, herbaceous succulent annual plant with a cosmopolitan distribution belonging to the Portulacaceae family. It is commonly known as purslane (USA and Australia), rigla (Egypt), pigweed (England), pourpier (France), and Ma-Chi-Xian (China) [1]. It is distributed widely in the tropical and subtropical areas of the world including many parts of the United States and is eaten extensively as a potherb and is added to soups and salads around the Mediterranean and tropical Asian countries [2]. Americans and aborigines of Australia grind the seeds of this plant into flour for use in mush and bread [3].Portulaca oleracea also provides a source of nutritional benefits owing to its rich omega-3 fatty acids and antioxidant properties [4].Portulaca oleraceahas been used as a folk medicine in many countries, acting as a febrifuge, antiseptic, vermifuge, and so forth [5]. It exhibits a wide range of pharmacological effects, including antibacterial [6], antiulcerogenic [7], anti-inflammatory [8], antioxidant [9], and wound-healing [10] properties. It is listed by the World Health Organization as one of the most used medicinal plants, and it has been given the term “Global Panacea” [11]. The Chinese folklore described it as “vegetable for long life” and it has been used for thousands of years in traditional Chinese Medicine [12, 13]. It is cold in nature and sour in taste and is used to cool the blood, stanch bleeding, clear heat, and resolve toxins. The dried aerial part of this plant is indicated for the treatment of fever, dysentery, diarrhoea, carbuncle, eczema and hematochezia, with a recommended dose of 9–15 g [14–16].Portulaca oleracea has a high potential to be used as human and animal food and to be utilized as a pharmacological agent in medicine. In this paper, phytochemistry and pharmacological activities of this plant are reviewed and its potential for further investigation, exploitation, and utilization are discussed.


## 2. Phytochemistry

Many constituents ofPortulaca oleracea have been isolated, including flavonoids, alkaloids, fatty acids, terpenoids, polysaccharides, vitamins, sterols, proteins, and minerals; these are listed in Table 1 and the chemical structures of the main compounds are presented in Figure 1.Table 1
Compounds isolated from purslane.
Classification
Chemical component
Part of plant
Reference
Flavonoids
Kaempferol (1)
Leaf and stem
[11]
Apigenin (2)
Leaf and stem
[11]
Luteolin (3)
Whole plant
[11]
Myricetin (4)
Whole plant
[11]
Quercetin (5)
Whole plant
[11]
Portulacanones A (6)
Aerial part
[18]
Portulacanones B (7)
Aerial part
[18]
Portulacanones C (8)
Aerial part
[18]
Portulacanones D (9)
Aerial part
[18]
2,2′-Dihydroxy-4′,6′-dimethoxychalcone (10)
Aerial parts
[18]
Genistein
Whole plant
[17]
Genistin
Whole plant
[17]
Alkaloids
Dopamine (11)
Stem, leaf and seed
[20]
Noradrenalin (12)
Stem, leaf and seed
[48]
Dopa
[21]
Oleraceins A (13)
Whole plant
[21]
Oleraceins B (14)
Whole plant
[21]
Oleraceins C (15)
Whole plant
[21]
Oleraceins D (16)
Whole plant
[21]
Oleraceins E (17)
Whole plant
[21]
Oleracins I
Stem
[21]
Oleracins II
Stem
[21]
Adenosine
Whole plant
[21]
N-trans-Feruloyltyramine (18)
Aerial part
[22]
(7′R)-N-Feruloylnormetanephrine (19)
Aerial part
[22]
1,5-Dimethyl-6-phenyl-1,2-dihydro-1,2,4-triazin-3(2H)-one (20)
Aerial part
[22]
(3R)-3,5-Bis(3-methoxy-4-hydroxyphenyl)-2,3-dihydro-2(1H)-pyridinone (21)
Aerial part
[22]
Thymine (22)
Aerial parts
[18]
Uracil (23)
Aerial parts
[18]
N-cis-Feruloyltyramine (24)
Aerial parts
[18]
N-trans-Feruloyloctopamine (25)
Aerial parts
[18]
N-cis-Feruloyloctopamine (26)
Aerial parts
[18]
Trollisine (27)
Aerial part
[49]
Aurantiamide (28)
Aerial part
[49]
Aurantiamide acetate (29)
Aerial part
[49]
Cyclo(L-tyrosinyl-L-tyrosinyl) (30)
Aerial part
[49]
1,5-Dimethyl-6-phenyl-1,6,3,4-tetrahydro-1,2,4-2(1H)-triazin (31)
Aerial part
[49]
Scopoletin
[50]
Terpenoids
Portuloside A (32)
Aerial part
[51]
Portuloside B (33)
Aerial part
[52]
(3S)-3-O-(β-D-Glucopyranosyl)-3,7-dimethylocta-1,6-dien-3-ol (34)
Aerial part
[52]
(3S)-3-O-(β-D-Glucopyranosyl)-3,7-dimethylocta-1,5-dien-3,7-diol (35)
Aerial part
[52]
Portulene (36)
Aerial part
[1]
Lupeol (37)
Aerial part
[1]
(2a,3a)-3-{[4-O-(β-D-Glucopyranosyl)-β-D-xylopyranosyl]oxy}-2,23-dihydroxy-30-methoxy-30-oxoolean-12-en-28-oic acid (38)
Aerial part
[25]
(2a,3a)-2,23,30-Trihydroxy-3-[(β-D-xylopyranosyl)oxy]olean-12-en-28-oic acid (39)
Aerial part
[25]
Friedelane
Aerial part
[25]
Organic acids
3-Quinolinecarboxylic acid (40)
Aerial parts
[18]
Indole-3-carboxylic acid (41)
Aerial parts
[18]
a-Linolenic acid
Leaf
[24]
Linoleic acid
Leaf
[26]
Palmitic acid
Leaf
[4]
Stearic acid
Leaf
[4]
Oleic acid
Leaf
[4]
p-Coumaric acid
Whole plant
[21]
Ferulic acid
Whole plant
[21]
Docosapentaenoic acid
Stem
[26]
Eicosapentaenoic acid
[53]
Docosahexaenoic acid
[53]
Catechol
[53]
Caffeic acid
Aerial part
[54]
Oxalic acid
Leaf
[2]
Lonchocarpic acid
[50]
Vitamins
Vitamin A
Leaf
[26]
Riboflavin
Leaf
[26]
Niacin
Leaf
[26]
Pyridoxine
Leaf
[26]
Vitamin C
Leaf
[26]
Folates
Leaf
[26]
Pantothenic acid
Leaf
[26]
Thiamin
Leaf
[26]
α-Tocopherol
Leaf
[4]
Hesperidin
Leaf
[55]
Minerals
Phosphorus
Root, stem and leaf
[3]
Iron
Root, stem and leaf
[3]
Manganese
Root, stem and leaf
[3]
Calcium
Root, stem and leaf
[3]
Copper
Root, stem and leaf
[3]
Zinc
Leaf
[26]
Selenium
Leaf
[26]
Magnesium
Leaf
[26]
Other compounds
Portulacerebroside A (42)
Aerial part
[56]
β-Sitosterol (43)
Aerial part
[1]
Daucosterol (44)
Aerial part
[1]
β-Carotene
Leaf
[4]
Glutathione
Leaf
[4]
Proline
Leaf
[57]
Melatonin
Leaf
[24]
1,4-Di-O-acetyl-2,3,5-tri-O-methyl-L-arabinitol
Leaf
[58]
1,4,5-Tri-O-acetyl-2,3-di-O-methyl-L-arabinitol
Leaf
[58]
1,5-Di-O-acetyl-2,3,4,6-tetra-O-methyl-D-galactitol
Leaf
[58]
1,4,5-Tri-O-acetyl-2,3,6-tri-O-methyl-D-galactitol
Leaf
[58]
1,3,4,5-Tetra-O-acetyl-2,6-di-O-methyl-D-galactitol
Leaf
[58]
Chlorophyll
[53]
Tannin
[53]
Isopimpinellin
[50]
Robustin
[50]
Bergapten
[50]Chemical structures of main compounds present inPortulaca oleracea.One of the most effective constituents present in Chinese Herbal Medicines are flavonoids which are biologically active and possess a wide range of pharmacological properties such as antibacterial, antivirus, anti-inflammation, and antioxidation properties. In thePortulaca oleracea plant, the flavonoids levels vary according to the part of the plant; the highest levels are present in the root followed by stem and the leaf; and seven different flavonoids are present in this plant, including kaempferol, myricetin, luteolin, apigenin, quercetin, genistein, and genistin [17]. However, only kaempferol and apigenin have been found in ethanolic extracts of leaves and stems, with the levels in the former being higher [11]. Portulacanones B–D, three homoisoflavonoids compounds, display selectively cytotoxic activities against three human cancer cell lines (SF-268, NCI-H460, and SGC-7901) [18]. Flavonoids are also widely present in foods such as fruits and vegetables [19].In addition to flavonoids, another important chemical found in this plant is alkaloids including dopa, dopamine, and noradrenalin. The content of dopamine and noradrenalin is higher in leaves compared to stem and seeds. The amount of dopamine and noradrenalin obtained from leaves varies according to the solvents used in the extraction process, suggesting that the levels of these compounds are dependent on the solvents used during the extraction process [20]. Oleraceins A, B, C, D, and E are cyclodopa alkaloids isolated from this plant [21] and several analytes such as (3R)-3,5-bis(3-methoxy-4-hydroxyphenyl)-2,3-dihydro-2(1H)-pyridinone and 1,5-dimethyl-6-phenyl-1,2-dihydro-1,2,4-triazin-3(2H)-one display cytotoxic activities against human cancer cells [22].Portulaca oleracea is also an excellent source of omega-3 fatty acids, which is usually present in oil and fat of fishes but not normally found in plants. Omega-3 fatty acids play an important role in the enhancement of immune function [23] and prevention and treatment of hypertension, coronary artery disease, cancer, and other inflammatory and autoimmune disorders [24]. It includes α-linolenic acid and linoleic acid, which are essential for normal growth, health promotion, and disease prevention in humans. Polysaccharides found inPortulaca oleracea are potential therapeutic agents for the treatment of diabetes mellitus owing to their modulation of blood lipids, metabolism, and decrease of blood glucose.Portulaca oleracea contains monoterpenes such as portulosides A and B, diterpenes such as portulene, and β-amyrin type triterpenoids [1, 25]; in addition, vitamins have also been isolated from the leaves of this plant. It contains the highest content of vitamin A which is a natural antioxidant playing an important role in vision, maintaining healthy mucus membranes and protecting against lung and oral cavity cancers among green leafy vegetables. This plant also contains ascorbic acid, α-tocopherol, and B-complex vitamins, for example, niacin, pyridoxine, and riboflavin [26]. Furthermore it is rich in minerals like phosphorus, manganese, icon, calcium selenium [3], and the amino acids isoleucine, proline, leucine, lysine, phenylalanine, methionine, cystine, valine, threonine, and tyrosine [2]. Many other constituents have also been isolated from this plant, such as β-carotene, glutathione, melatonin, portulacerebroside A, catechol, and bergapten.


## 3. Pharmacology

Over the past decades, numerous researchers have investigated the pharmacological activities ofPortulaca oleracea. This review provides a comprehensive summary of the main pharmacological properties which are presented below.


### 3.1. Neuroprotective Activity

Administration ofPortulaca oleracea can scavenge free radicals and antagonize rotenone-induced neurons apoptosis, dopamine depletion, and complex-I inhibition in striatum of rats, suggesting thatPortulaca oleracea may be a potential neuroprotective candidate against Parkinson’s disease [23]. The extract ofPortulaca oleracea (EP) protects nerve tissue/cells from hypoxic damage probably by elevation of glycolysis, EPO, and hypoxia inducible factor-1 expression levels [27]. The ethanol extract decreases the activity of caspase-3 in neuron whilst reducing serum levels of neuron specific enolase in hypoxia mice and the pathological damages caused by hypoxia. In these studies, an increase in the neuron viability and an induction in the mRNA and protein expression of endogenous erythropoietin have also been reported. Thus, the stabilization of hypoxia inducible factor-1 α expression is associated with the neuroprotective effects of EP against hypoxia injury by eliciting endogenous erythropoietin expression [28]. β-Cyanin evidently inhibits D-galactose-induced neurotoxicity in mice, which at the doses of 50 and 100 mg/kg upregulates the activities of superoxide dismutases, catalase, glutathione reductase, and glutathione peroxidase, whilst reducing the level of the lipid peroxidation product malondialdehyde in the brain of D-galactose-treated mice. When compared to vitamin C, β-cyanin play a more pronounced effect on alleviating cognition deficits in mice [29]. The total alkaloidal extracts from 31 traditional Chinese Herbal Medicines were tested for their acetylcholinesterase (AChE) inhibitory activities by Ellman’s method and modified TLC bioautographic assay. As a result, the alkaloidal extract ofPortulaca oleracea significantly inhibited AChE activity at a final concentration of 100 μg/mL with the IC50 value of 29.4 μg/mL. The use of AChE inhibitors has been a promising treatment strategy for Alzheimer’s disease (AD); therefore,Portulaca oleracea may be an effective agent for the prophylaxis and treatment of AD [30].


### 3.2. Antidiabetic Activity

Portulaca oleracea attenuates body weight, serum free fatty acids, and hyperinsulinemia. It also increases insulin sensitivity and ameliorates impaired glucose tolerance and lipid metabolism in rats with type 2 diabetes mellitus induced by injection of streptozotocin (25 mg/kg) and feeding of high calorie forage, suggesting thatPortulaca oleracea alleviates insulin resistance [31]. Administration of the seeds powder (5 g × 2/day) increases high density lipoprotein cholesterol (HDLC) and albumin, while lowering the levels of serum total cholesterol, triglycerides, low density lipoprotein cholesterol (LDLC), liver gamma glutamyl transaminase (GGT), alanine transaminase (ALT), aspartate transaminase (AST), total and direct bilirubin, fasting and postprandial blood glucose, insulin, body weight, and body mass index in type 2 diabetic subjects. There were no differences in these results compared to the data obtained with metformin treatment (1500 mg/day) except for LDLC, HDLC, and alkaline phosphatase (ALP) levels, suggesting thatPortulaca oleracea seeds are valuable and effective as an adjunctive and alternative therapy for the treatment of type 2 diabetes mellitus [32].The aqueous extract ofPortulaca oleracea also prevents diabetic vascular inflammation, hyperglycemia, and diabetic endothelial dysfunction in type 2 diabetic db/db mice, suggesting its protective role against diabetes and related vascular complications [33]. The crude polysaccharide extract of this plant also lowers blood glucose and modulates the metabolism of blood lipids and glucose in alloxan-induced diabetic mice [34], whilst decreasing the levels of total cholesterol, triglycerides, and fasting blood glucose in type 2 diabetic mice [32].


### 3.3. Antioxidant Activity

The antioxidant property ofPortulaca oleracea is attributed to its constituents, such as gallotannins, omega-3 fatty acids, ascorbic acid, α-tocopherols, kaempferol, quercetin, and apigenin [8, 16, 17]. The single cell gel electrophoresis assay (comet assay), which is an simple, rapid, and inexpensive method for measuring DNA strand breaks, confirmed that the aqueous extract significantly alleviated hydrogen peroxide-induced oxidative DNA lesions in human lymphocytes, whereas the ethanolic extract had no effects, which may be associated with the antioxidant constituents contained in the aqueous extract [35]. The aqueous extract decreases high fat diet-elicited oxidative damage by modulating blood and liver antioxidant enzyme activities, elevating leptin/β-actin and liver PPAR a/β-actin and inhibiting the protein expression of p-PERK and the FAS mRNA expression of liver and spleen in mice [9]. In another study, the aqueous extract at a concentration range of 100, 150, 200, and 400 μg/mL and the ethanolic extract at a range of 1200 and 1800 μg/mL, respectively, exerted cytoprotective effects on 2,2′-azobis hydrochloride-induced hemolytic damages of RBCs in a concentration-dependent manner [36].


### 3.4. Anticancer Activity

Polysaccharides fromPortulaca oleracea display several biological activities, such as anticancer, antioxidation, anti-inflammation, and immunity enhancing properties [37–40]. Polysaccharides evidently scavenge the accumulation of free radicals and modulate immunity functions of rats with ovarian cancer [41]. Sulfated derivatives of POP, a water-soluble polysaccharide isolated fromPortulaca oleracea, have a suppressive effect on the growth of HeLa and HepG2 cellsin vitro, suggesting that the sulfation of POP increases the cytotoxicity in tumor cells [42]. In addition to polysaccharides, other bioactive compounds such as cerebrosides, homoisoflavonoids, and alkaloids also showin vitro cytotoxic activities against human cancer cell lines. Portulacerebroside A stimulates human liver cancer HCCLM3 cell apoptosis via the activation of the p38 MAPK- and JNK-triggered mitochondrial death pathway [43] and 2,2′-dihydroxy-4′,6′-dimethoxychalcone is more active against cell line SGC-7901 with an IC50 value of 1.6 ug/mL than mitomycin C which has an IC50 value of 13.0 ug/mL. Portulacanones B is active against SGC-7901 cell lines with an IC50 value of 16.2 ug/mL, which is very close to the value obtained with mitomycin C. 2,2′-Dihydroxy-4′,6′-dimethoxychalcone is moderately active against K-562 cells with an IC50 value of 24.6 ug/mL and portulacanones B–D show selective cytotoxic activity against SF-268 and/or NCI-H460 cells with IC50 values of 14.3–20.1 ug/mL [18]. N-trans-Feruloyltyramine, (7′R)-N-feruloylnormetanephrine, 1,5-dimethyl-6-phenyl-1,2-dihydro-1,2,4-triazin-3(2H)-one, and (3R)-3,5-bis(3-methoxy-4-hydroxyphenyl)-2,3-dihydro-2(1H)-pyridinone have weak bioactivities against K562 with IC50 values of 222.77, 66.94, 90.09, and 41.52 umol/L, respectively, and moderate bioactivities against A549 with IC50 values of 28.80, 21.76, 24.54, and 37.20 umol/L, respectively [22]. These studies demonstrate thatPortulaca oleracea has a potential application in the treatment of cancer.


### 3.5. Antimicrobial

Portulaca oleracea possesses antibacterial, antifungal, and antiviral activities as revealed by its antifungal effect against dermatophytes of the generaTrichophyton [44]. A pectic polysaccharide isolated from the aerial part of this plant displays antiherpes property against simplex virus type 2 which is due to the inhibition of virus penetration and not virus adsorption [45]. A 70% methyl alcohol extract ofPortulaca oleracea shows antibacterial activity against the Gram-negative stains:Escherichia coli,Pseudomonas aeruginosa, andNeisseria gonorrhea with inhibition zones of 14, 15, and 15 mm, respectively, and the Gram-positive strains:Staphylococcus aureus,Bacillus subtilis, andStreptococcus faecalis with inhibition zones of 13, 14, and 15 mm, respectively, as well as antifungal activity againstCandida albicans with inhibition zone of 12 mm [1].


### 3.6. Anti-Inflammatory Activity

Pretreatment with the aqueous extract ofPortulaca oleracea inhibits tumor necrosis factor- (TNF-) α-induced production of intracellular reactive oxygen species (ROS) and overexpression of intercellular adhesion molecule- (ICAM-) 1, vascular cell adhesion molecule (VCAM)-1, and E-selectin in human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. This extract also suppresses the translocation of nuclear factor κB (NF-κB) p65 to the nucleus, TNF-α-induced NF-κB binding, and the degradation of inhibitor molecule (IκB)α. Furthermore, an inhibition in the adhesion of HL-60 cells to TNF-α-induced HUVECs and TNF-α-induced mRNA expression of interleukin- (IL-) 8 and monocyte chemoattractant protein- (MCP-) 1 is also observed. The aqueous extract ofPortulaca oleracea may also play an important role in the suppression of the vascular inflammatory process related to the development of atherosclerosis [46].


### 3.7. Antiulcerogenic Activity

Aqueous and ethanolic extracts ofPortulaca oleracea at 0.8 g/kg and 1.4 g/kg, respectively, can reduce the severity of HCl-induced gastric ulcers in a dose-dependent manner; this is comparable to the effect observed with sucralfate 0.1 g/kg. In addition, the aqueous extract (0.56 and 0.8 g/kg) and the ethanolic extract (0.8 and 1.4 g/kg) display suppression of lesions induced by absolute ethanol. The oral and intraperitoneal doses of both extracts dose-dependently increase the pH of gastric juice in mice with pylorus ligation. Thus,Portulaca oleracea holds great promise as an effective therapeutic agent for gastrointestinal diseases due to its gastroprotective activity [7].


### 3.8. Hepatoprotective Activity

Intraperitoneal administration of CCl4 elicits liver injury in rats, which notably upregulates the levels of total bilirubin and serum hepatic marker enzymes, including glutamate pyruvate transaminase (GPT) and glutamate oxaloacetate transaminase (GOT). A 70% alcohol extract ofPortulaca oleracea significantly reverses the increase in hepatic marker enzymes and total bilirubin levels, confirming the hepatoprotective activity of this plant [1].


### 3.9. Other Activities

The ethanol extract fromPortulaca oleracea at a concentration range of 100, 200, and 400 mg/kg, respectively, displays a dose-dependent effect in prolonging the survival time of mice in hypoxic models, including closed normobaric hypoxia and potassium cyanide or sodium nitrite toxicosis. This extract also enhances the activities of phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in glycolysis and the level of adenosine triphosphate of mouse cortices in hypoxia models [12]. The preliminary wound healing activity ofPortulaca oleracea has been appraised inMus musculusJVI-1 and it has been shown that a fresh crude extract significantly accelerates the wound healing course by the stimulation of wound contraction and downregulation of the surface area of the excision wound [10].Portulaca oleracea also has the ability to accumulate Se even at the shortest time span of 42 days, and hence it can perform the dual functions of preventing the occurrence of Se deficiency linked diseases such as Keshan and Kashin-Beck diseases [47].


## 3.1. Neuroprotective Activity

Administration ofPortulaca oleracea can scavenge free radicals and antagonize rotenone-induced neurons apoptosis, dopamine depletion, and complex-I inhibition in striatum of rats, suggesting thatPortulaca oleracea may be a potential neuroprotective candidate against Parkinson’s disease [23]. The extract ofPortulaca oleracea (EP) protects nerve tissue/cells from hypoxic damage probably by elevation of glycolysis, EPO, and hypoxia inducible factor-1 expression levels [27]. The ethanol extract decreases the activity of caspase-3 in neuron whilst reducing serum levels of neuron specific enolase in hypoxia mice and the pathological damages caused by hypoxia. In these studies, an increase in the neuron viability and an induction in the mRNA and protein expression of endogenous erythropoietin have also been reported. Thus, the stabilization of hypoxia inducible factor-1 α expression is associated with the neuroprotective effects of EP against hypoxia injury by eliciting endogenous erythropoietin expression [28]. β-Cyanin evidently inhibits D-galactose-induced neurotoxicity in mice, which at the doses of 50 and 100 mg/kg upregulates the activities of superoxide dismutases, catalase, glutathione reductase, and glutathione peroxidase, whilst reducing the level of the lipid peroxidation product malondialdehyde in the brain of D-galactose-treated mice. When compared to vitamin C, β-cyanin play a more pronounced effect on alleviating cognition deficits in mice [29]. The total alkaloidal extracts from 31 traditional Chinese Herbal Medicines were tested for their acetylcholinesterase (AChE) inhibitory activities by Ellman’s method and modified TLC bioautographic assay. As a result, the alkaloidal extract ofPortulaca oleracea significantly inhibited AChE activity at a final concentration of 100 μg/mL with the IC50 value of 29.4 μg/mL. The use of AChE inhibitors has been a promising treatment strategy for Alzheimer’s disease (AD); therefore,Portulaca oleracea may be an effective agent for the prophylaxis and treatment of AD [30].


## 3.2. Antidiabetic Activity

Portulaca oleracea attenuates body weight, serum free fatty acids, and hyperinsulinemia. It also increases insulin sensitivity and ameliorates impaired glucose tolerance and lipid metabolism in rats with type 2 diabetes mellitus induced by injection of streptozotocin (25 mg/kg) and feeding of high calorie forage, suggesting thatPortulaca oleracea alleviates insulin resistance [31]. Administration of the seeds powder (5 g × 2/day) increases high density lipoprotein cholesterol (HDLC) and albumin, while lowering the levels of serum total cholesterol, triglycerides, low density lipoprotein cholesterol (LDLC), liver gamma glutamyl transaminase (GGT), alanine transaminase (ALT), aspartate transaminase (AST), total and direct bilirubin, fasting and postprandial blood glucose, insulin, body weight, and body mass index in type 2 diabetic subjects. There were no differences in these results compared to the data obtained with metformin treatment (1500 mg/day) except for LDLC, HDLC, and alkaline phosphatase (ALP) levels, suggesting thatPortulaca oleracea seeds are valuable and effective as an adjunctive and alternative therapy for the treatment of type 2 diabetes mellitus [32].The aqueous extract ofPortulaca oleracea also prevents diabetic vascular inflammation, hyperglycemia, and diabetic endothelial dysfunction in type 2 diabetic db/db mice, suggesting its protective role against diabetes and related vascular complications [33]. The crude polysaccharide extract of this plant also lowers blood glucose and modulates the metabolism of blood lipids and glucose in alloxan-induced diabetic mice [34], whilst decreasing the levels of total cholesterol, triglycerides, and fasting blood glucose in type 2 diabetic mice [32].


## 3.3. Antioxidant Activity

The antioxidant property ofPortulaca oleracea is attributed to its constituents, such as gallotannins, omega-3 fatty acids, ascorbic acid, α-tocopherols, kaempferol, quercetin, and apigenin [8, 16, 17]. The single cell gel electrophoresis assay (comet assay), which is an simple, rapid, and inexpensive method for measuring DNA strand breaks, confirmed that the aqueous extract significantly alleviated hydrogen peroxide-induced oxidative DNA lesions in human lymphocytes, whereas the ethanolic extract had no effects, which may be associated with the antioxidant constituents contained in the aqueous extract [35]. The aqueous extract decreases high fat diet-elicited oxidative damage by modulating blood and liver antioxidant enzyme activities, elevating leptin/β-actin and liver PPAR a/β-actin and inhibiting the protein expression of p-PERK and the FAS mRNA expression of liver and spleen in mice [9]. In another study, the aqueous extract at a concentration range of 100, 150, 200, and 400 μg/mL and the ethanolic extract at a range of 1200 and 1800 μg/mL, respectively, exerted cytoprotective effects on 2,2′-azobis hydrochloride-induced hemolytic damages of RBCs in a concentration-dependent manner [36].


## 3.4. Anticancer Activity

Polysaccharides fromPortulaca oleracea display several biological activities, such as anticancer, antioxidation, anti-inflammation, and immunity enhancing properties [37–40]. Polysaccharides evidently scavenge the accumulation of free radicals and modulate immunity functions of rats with ovarian cancer [41]. Sulfated derivatives of POP, a water-soluble polysaccharide isolated fromPortulaca oleracea, have a suppressive effect on the growth of HeLa and HepG2 cellsin vitro, suggesting that the sulfation of POP increases the cytotoxicity in tumor cells [42]. In addition to polysaccharides, other bioactive compounds such as cerebrosides, homoisoflavonoids, and alkaloids also showin vitro cytotoxic activities against human cancer cell lines. Portulacerebroside A stimulates human liver cancer HCCLM3 cell apoptosis via the activation of the p38 MAPK- and JNK-triggered mitochondrial death pathway [43] and 2,2′-dihydroxy-4′,6′-dimethoxychalcone is more active against cell line SGC-7901 with an IC50 value of 1.6 ug/mL than mitomycin C which has an IC50 value of 13.0 ug/mL. Portulacanones B is active against SGC-7901 cell lines with an IC50 value of 16.2 ug/mL, which is very close to the value obtained with mitomycin C. 2,2′-Dihydroxy-4′,6′-dimethoxychalcone is moderately active against K-562 cells with an IC50 value of 24.6 ug/mL and portulacanones B–D show selective cytotoxic activity against SF-268 and/or NCI-H460 cells with IC50 values of 14.3–20.1 ug/mL [18]. N-trans-Feruloyltyramine, (7′R)-N-feruloylnormetanephrine, 1,5-dimethyl-6-phenyl-1,2-dihydro-1,2,4-triazin-3(2H)-one, and (3R)-3,5-bis(3-methoxy-4-hydroxyphenyl)-2,3-dihydro-2(1H)-pyridinone have weak bioactivities against K562 with IC50 values of 222.77, 66.94, 90.09, and 41.52 umol/L, respectively, and moderate bioactivities against A549 with IC50 values of 28.80, 21.76, 24.54, and 37.20 umol/L, respectively [22]. These studies demonstrate thatPortulaca oleracea has a potential application in the treatment of cancer.


## 3.5. Antimicrobial

Portulaca oleracea possesses antibacterial, antifungal, and antiviral activities as revealed by its antifungal effect against dermatophytes of the generaTrichophyton [44]. A pectic polysaccharide isolated from the aerial part of this plant displays antiherpes property against simplex virus type 2 which is due to the inhibition of virus penetration and not virus adsorption [45]. A 70% methyl alcohol extract ofPortulaca oleracea shows antibacterial activity against the Gram-negative stains:Escherichia coli,Pseudomonas aeruginosa, andNeisseria gonorrhea with inhibition zones of 14, 15, and 15 mm, respectively, and the Gram-positive strains:Staphylococcus aureus,Bacillus subtilis, andStreptococcus faecalis with inhibition zones of 13, 14, and 15 mm, respectively, as well as antifungal activity againstCandida albicans with inhibition zone of 12 mm [1].


## 3.6. Anti-Inflammatory Activity

Pretreatment with the aqueous extract ofPortulaca oleracea inhibits tumor necrosis factor- (TNF-) α-induced production of intracellular reactive oxygen species (ROS) and overexpression of intercellular adhesion molecule- (ICAM-) 1, vascular cell adhesion molecule (VCAM)-1, and E-selectin in human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. This extract also suppresses the translocation of nuclear factor κB (NF-κB) p65 to the nucleus, TNF-α-induced NF-κB binding, and the degradation of inhibitor molecule (IκB)α. Furthermore, an inhibition in the adhesion of HL-60 cells to TNF-α-induced HUVECs and TNF-α-induced mRNA expression of interleukin- (IL-) 8 and monocyte chemoattractant protein- (MCP-) 1 is also observed. The aqueous extract ofPortulaca oleracea may also play an important role in the suppression of the vascular inflammatory process related to the development of atherosclerosis [46].


## 3.7. Antiulcerogenic Activity

Aqueous and ethanolic extracts ofPortulaca oleracea at 0.8 g/kg and 1.4 g/kg, respectively, can reduce the severity of HCl-induced gastric ulcers in a dose-dependent manner; this is comparable to the effect observed with sucralfate 0.1 g/kg. In addition, the aqueous extract (0.56 and 0.8 g/kg) and the ethanolic extract (0.8 and 1.4 g/kg) display suppression of lesions induced by absolute ethanol. The oral and intraperitoneal doses of both extracts dose-dependently increase the pH of gastric juice in mice with pylorus ligation. Thus,Portulaca oleracea holds great promise as an effective therapeutic agent for gastrointestinal diseases due to its gastroprotective activity [7].


## 3.8. Hepatoprotective Activity

Intraperitoneal administration of CCl4 elicits liver injury in rats, which notably upregulates the levels of total bilirubin and serum hepatic marker enzymes, including glutamate pyruvate transaminase (GPT) and glutamate oxaloacetate transaminase (GOT). A 70% alcohol extract ofPortulaca oleracea significantly reverses the increase in hepatic marker enzymes and total bilirubin levels, confirming the hepatoprotective activity of this plant [1].


## 3.9. Other Activities

The ethanol extract fromPortulaca oleracea at a concentration range of 100, 200, and 400 mg/kg, respectively, displays a dose-dependent effect in prolonging the survival time of mice in hypoxic models, including closed normobaric hypoxia and potassium cyanide or sodium nitrite toxicosis. This extract also enhances the activities of phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in glycolysis and the level of adenosine triphosphate of mouse cortices in hypoxia models [12]. The preliminary wound healing activity ofPortulaca oleracea has been appraised inMus musculusJVI-1 and it has been shown that a fresh crude extract significantly accelerates the wound healing course by the stimulation of wound contraction and downregulation of the surface area of the excision wound [10].Portulaca oleracea also has the ability to accumulate Se even at the shortest time span of 42 days, and hence it can perform the dual functions of preventing the occurrence of Se deficiency linked diseases such as Keshan and Kashin-Beck diseases [47].


## 4. Conclusion

Portulaca oleracea is of considerable importance to the food industry and also possesses a wide spectrum of pharmacological properties such as neuroprotective, antimicrobial, antidiabetic, antioxidant, anti-inflammatory, antiulcerogenic, and anticancer activities, which are associated with its diverse chemical constituents, including flavonoids, alkaloids, polysaccharides, fatty acids, terpenoids, sterols, proteins, vitamins, and minerals.Although bioactivities of extracts or compounds isolated fromPortulaca oleracea are substantiated by usingin vitro andin vivo studies including animal models and cell culture studies, the mechanisms of action have not been addressed. Hence, more mechanistic studies are required beforePortulaca oleracea can be considered for further clinical use. This review concludes thatPortulaca oleracea is an edible and a medicinal plant which is important to the food industry and may also have a significant role to play in health care provided that adequate studies are conducted.



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