# Nursing Effect of Zinc Oxide Nanoantibacterial Materials after Adrenalectomy

Authors: Yiyang Fan; Jingjing Liu; Mingrui Fan
Journal: Journal of Nanomaterials
 (2022)

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## Abstract

Laparoscopic adrenalectomy (RLA) is widely used for adrenal tumors, cancer, cortical hyperplasia, etc. Therefore, how to care for patients after surgery is a necessary means to speed up the rapid healing of surgical wounds and reduce the incidence of related complications. To solve the problem in traditional wound treatment, antibacterial dressings are used, and most of the auxiliary materials need to be replaced frequently, or there is serious adhesion to the wound, which causes secondary wound infection. The methods used to study in this work report a new long-acting antibacterial and breathable wound dressing based on nanoscale zinc oxide nanoparticles (ZnO NPs) in the care of adrenalectomy; the clinical effects of ZnO NP-based antibacterial dressings were systematically evaluated in terms of patients’ wound healing rate (%), infection rate (%), healing time (d), and the number of dressing changes. The results revealed that the wound healing rate of patients with ZnO NP-based antibacterial dressing was as high as 16.67% and the incidence of adverse reactions was only 26.19%, which was 40.48% lower than traditional dressings. Such promising antibacterial dressings are expected to be greatly used in the medical field in the future.


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## Body

## 1. Introduction

The main purpose of any surgical treatment is to complete the resection of the lesion and wound healing as quickly as possible to avoid infection. Minimally invasive RLA also inevitably leaves a wound, and the slow healing process of the wound is the accumulation area of high infectious inflammatory load [1, 2]. The factors that increase the delay of wound healing mainly involve the formation of microbial complexes, types, and biofilms at the wound, but they are not limited to these factors. The main microorganisms concentrated on the wound surface are Pseudomonas, Staphylococcus, Streptococcus, etc., which have been confirmed by various microbiological methods and microbial spectrum. These microbial populations show increasingly strong resistance to traditional antibacterial dressings. Therefore, new antibacterial dressings are waiting to be developed to relieve the distress of postoperative wound care for patients [3].Nanograde zinc oxide nanoparticles (ZnO NPs) belong to the white hexagonal crystal system, with a particle size distribution in the range of 1.00−100 nm. It has extremely high chemical, catalytic, and photocatalytic activities [4]. In addition, ZnO NPs have antibacterial functions similar to ultraviolet rays and are widely used in antibacterial drugs, dressings, test papers, and other fields [5]. Antibacterial compounds such as silver sulfadiazine and silver nitrate developed in modern times enjoy the reputation of the “gold standard” in the treatment of inflammatory wounds. However, these antibacterial compounds are slow in accelerating the wound healing process, have obvious cytotoxic effects on host cells, and easily leave scars. Compared with these classic antibacterial compounds, the novel ZnO NPs exhibit excellent antibacterial function at an appropriate concentration and do not have any side effects on normal cells, which have been confirmed by many experiments [6]. Zn2+ particles are released through ZnO NP cell mediator to promote the formation of keratin and recruit repair cells to gather at the wound, thereby speeding up wound healing [7–9].The optimum dressing should be flexible, mechanically strong, tear-resistant, and porous [10, 11]. It should also be able to adequately absorb extravasated fluid from the wound surface without sticking to it [12]. The antibacterial dressing must also block external germs and prevent their development and reproduction on the wound surface. The antimicrobial dressing must be permeable to allow gas exchange with the outside environment to promote wound healing cell proliferation [13–15]. Because of this, polyvinyl alcohol (PVA), which has good biocompatibility, bioadhesion, and nontoxicity, was chosen as the coating material for the ZnO NP antibacterial agent in this study in order to comply with the demanding standards of medical antibacterial dressings. The chemical response of PVA can be modified by modifying its simple chemical structure, which is based on its basic chemical structure. It is surprising to learn that, aside from its rubber-like qualities (viscoelasticity), PVA exhibits a high degree of swelling in either water or biological fluids, which allows it to mimic real tissues and be adequately tolerated by the human body [16, 17].Bacterial cellulose (BC) is a porous network nanoscale biopolymer synthesized by microbial fermentation. Because of its excellent biocompatibility, adaptability, and biodegradability, it is possible to construct a new type of wound medical dressing [18, 19]. BC is usually a polysaccharide polymer synthesized by bacteria such as Acetobacter, Gluconobacter, Oomycetes, Agrobacterium, Sarcobacteria, and Enterobacter [20]. As far as we know, the study of ZnO NPs in BC and PVA composite gel medical antibacterial dressings has not been reported, and some related studies only consider a single influencing factor and lead to inevitable defects. For example, Wahid et al. [21] only emphasized the antibacterial properties of the BC/ZnO nanocomposite film, but the cytotoxicity of the dressing in the actual application process was not considered in depth. Therefore, when designing wound antibacterial dressings, one should not blindly pursue antibacterial effects. It is necessary to balance the antibacterial properties and potential cytotoxicity of wound dressings from the overall perspective.Surgical therapy is aimed at removing the lesion and encouraging wound healing as early as possible to prevent infection. The cytotoxicity of the dressing during application was not extensively evaluated prior to the operation. When making antibacterial wound dressings, keep in mind that antibacterial characteristics are not the primary consideration.This work systematically explores the reasonable ratio of antibacterial gel dressings (ZnO-BC/PVA) for postadrenalectomy wound care based on BC and PVA polymer composite network coated with ZnO NPs, aiming to improve the antibacterial performance of the dressing at the same time; it reduces its potential toxic effect on cells, thereby constructing a new type of air-permeable wound surface antibacterial care dressing and exploring its actual care effect in clinical applications.


## 2. Materials and Methods

### 2.1. Reagent

Zinc oxide nanoparticles (ZnO NPs, average size:50±10 nm) and polyvinyl alcohol (PVA, alcoholysis degree: 98.0–99.0 mol%, viscosity: 3.2–3.8 mPa·s) were purchased from Sigma-Aldrich (St. Louis, Missouri, United States). Bacterial cellulose (BC, gel state, about 50−100 nm in diameter, 20 μm in length) was provided by Shanghai Guangyu Biotechnology Co., Ltd. (Shanghai, China). Sodium periodate (NaIO4, 99.0%), ethylene glycol (C2H6O2, 99.5%), zinc chloride (ZnCl2, 99.0%), and sodium hydroxide (NaOH, 99.9%) were purchased from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China).


### 2.2. Instrument

The surface morphology and microstructure of ZnO-BC/PVA were observed by a scanning electron microscope (SEM, JSM-6701F, Japan). Purified water equipment (UPH-I-5, UPH Instrument Equipment Co., Ltd., Shaanxi, China) is used to produce deionized water. All experiments use deionized water as the solvent, and all reagents can be used without further purification.


### 2.3. ZnO-BC/PVA Preparation

The antibacterial gel ZnO-BC/PVA was prepared based on a three-step method. Initially, 100 g of BC was dispersed in ultrapure water (500 mL) and stored at room temperature for 24 h. Then, the BC gel was transferred to 0.10 M NaOH solution and soaked for 24 h to remove the remaining impurities in the BC gel. Wash the BC gel with ultrapure water several times until itspH=7.0, and store it in a 4.0°C freezer for later use.The above BC was dispersed in NaIO4 (1 : 2 w/w), and the flask was wrapped in a tin foil to react for 12 h at 50°C in the dark. After the reaction was completed, 100 mL of 0.10 M C2H6O2 was added to the reaction system and the reaction continued for 2.0 h. The obtained BC mixture was soaked in ultrapure water for 3.0 h to remove excess NaIO4 and C2H6O2. PVA was dissolved in distilled water at 80°C for about 6.0 h to obtain a 15.0% PVA dispersion, and then, the oxidized BC gel was placed in a 15.0% PVA solution and allowed to stand for 24 h to obtain a BC/PVA gel. Finally, the BC/PVA was washed three times with ultrapure water to remove unreacted PVA.Add 200 mL of 0.05 M ZnCl2 solution to the BC/PVA gel, and soak for 30 h at room temperature to obtain a ZnO-BC/PVA gel. Then, take out the gel and rinse it with pure water 3 times. Finally, the gel was soaked in 0.20 M NaOH for 12 h and washed with pure water to pH=7.0.


### 2.4. Clinical Application of ZnO-BC/PVA

#### 2.4.1. General Patient Information

Eighty-one patients undergoing adrenalectomy were selected, including 41 males and 40 females. The age distribution ranged from 31 to 62 years, and the disease course was in the distribution range of one to three years. The patients were randomly divided into two groups, namely, silver sulfadiazine (SS) and ZnO NP-based antibacterial dressing group (ZnO-BC/PVA). And the baseline data of each group of patients is recorded in Table1.Table 1
General patient information.
ProjectSS group (n=39)ZnO-BC/PVA group (n=42)SexMale2324Female1618Age (years)47.52±5.6748.29±4.82Course of disease (years)1.68±0.791.74±0.83Tumor diameter (mm)3.21±0.873.19±0.74Disease siteLeft1921Right2021


#### 2.4.2. Inclusion and Exclusion Criteria

The inclusion criteria for this work are as follows: the patient’s cognitive function is normal, and there is no redness, swelling, or purulent discharge on the wound after adrenalectomy; the patient and his family are professionally trained and qualified for home treatment; the study adopts the principle of patient voluntariness; all patients have signed informed consent. The exclusion criteria include patients with a history of infection in the recent (1.0–3 months) and other clinical trials; those with discomfort or allergies to ZnO NP-based antibacterial dressings; patients with critical illness; and the inability to evaluate drugs due to the use of other drugs. Effective patients can withdraw from this study when they have serious adverse reactions.


#### 2.4.3. Nursing Plan

Disinfect the wounds of the two groups of patients, implement the corresponding medical antibacterial dressing treatment, and guide the patients to change the dressing operation (Figure1). In addition, pay close attention to the patient’s psychological state and wound healing, and provide timely guidance. Provide scientific guidance on diet, and urge patients to strengthen nutrition and eat high-calcium, high-vitamin, and high-protein foods to speed up wound healing. Record the bleeding, exudation, pain, and other conditions of the wounds of the two groups of patients, and take targeted treatment measures. For instance, when replacing the antibacterial dressing, use 0.50% povidone-iodine and normal saline to treat the wound repeatedly before changing the dressing. The frequency of dressing change between the two groups of patients was adjusted according to the severity of exudation and pain, and wound secretions were collected regularly for in vitro culture to identify bacteria accumulated on the wound and evaluate wound healing.Figure 1
Application of ZnO-BC/PVA antibacterial nursing dressing in nursing after adrenalectomy.


#### 2.4.4. Measurement Index

After three weeks of antibacterial dressing treatment, the total effective rate, VSA score and nursing satisfaction of the two groups of patients were counted. Among them, the wound is fully healed and no scar is counted as healed; the wound with a reduction of 30.0% and a smaller scar is counted as significant; the wound with a reduction of 50% and scars is counted as effective; and the wound with a smaller reduction rate is counted as ineffective. The timeliness of the two dressings was evaluated by counting the number of dressing changes and the interval between patients, and the number of bacteria on the wounds of the two groups of patients was detected to evaluate the antibacterial properties of the dressings. Statistical analysis was made on the adverse reactions and complication rates of the two medical antibacterial dressings.


### 2.5. Statistical Methods

Use SPSS 22.0 to process, and analyze the data involved in this research. Quantitative variables are expressed as themean±standarddeviationx¯±s, and qualitative variables are described by frequency distribution and percentage (%). The t-test is used for the analysis of quantitative variables, and the analysis of qualitative variables uses the χ2 test. P<0.05 indicates statistical significance.


## 2.1. Reagent

Zinc oxide nanoparticles (ZnO NPs, average size:50±10 nm) and polyvinyl alcohol (PVA, alcoholysis degree: 98.0–99.0 mol%, viscosity: 3.2–3.8 mPa·s) were purchased from Sigma-Aldrich (St. Louis, Missouri, United States). Bacterial cellulose (BC, gel state, about 50−100 nm in diameter, 20 μm in length) was provided by Shanghai Guangyu Biotechnology Co., Ltd. (Shanghai, China). Sodium periodate (NaIO4, 99.0%), ethylene glycol (C2H6O2, 99.5%), zinc chloride (ZnCl2, 99.0%), and sodium hydroxide (NaOH, 99.9%) were purchased from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China).


## 2.2. Instrument

The surface morphology and microstructure of ZnO-BC/PVA were observed by a scanning electron microscope (SEM, JSM-6701F, Japan). Purified water equipment (UPH-I-5, UPH Instrument Equipment Co., Ltd., Shaanxi, China) is used to produce deionized water. All experiments use deionized water as the solvent, and all reagents can be used without further purification.


## 2.3. ZnO-BC/PVA Preparation

The antibacterial gel ZnO-BC/PVA was prepared based on a three-step method. Initially, 100 g of BC was dispersed in ultrapure water (500 mL) and stored at room temperature for 24 h. Then, the BC gel was transferred to 0.10 M NaOH solution and soaked for 24 h to remove the remaining impurities in the BC gel. Wash the BC gel with ultrapure water several times until itspH=7.0, and store it in a 4.0°C freezer for later use.The above BC was dispersed in NaIO4 (1 : 2 w/w), and the flask was wrapped in a tin foil to react for 12 h at 50°C in the dark. After the reaction was completed, 100 mL of 0.10 M C2H6O2 was added to the reaction system and the reaction continued for 2.0 h. The obtained BC mixture was soaked in ultrapure water for 3.0 h to remove excess NaIO4 and C2H6O2. PVA was dissolved in distilled water at 80°C for about 6.0 h to obtain a 15.0% PVA dispersion, and then, the oxidized BC gel was placed in a 15.0% PVA solution and allowed to stand for 24 h to obtain a BC/PVA gel. Finally, the BC/PVA was washed three times with ultrapure water to remove unreacted PVA.Add 200 mL of 0.05 M ZnCl2 solution to the BC/PVA gel, and soak for 30 h at room temperature to obtain a ZnO-BC/PVA gel. Then, take out the gel and rinse it with pure water 3 times. Finally, the gel was soaked in 0.20 M NaOH for 12 h and washed with pure water to pH=7.0.


## 2.4. Clinical Application of ZnO-BC/PVA

### 2.4.1. General Patient Information

Eighty-one patients undergoing adrenalectomy were selected, including 41 males and 40 females. The age distribution ranged from 31 to 62 years, and the disease course was in the distribution range of one to three years. The patients were randomly divided into two groups, namely, silver sulfadiazine (SS) and ZnO NP-based antibacterial dressing group (ZnO-BC/PVA). And the baseline data of each group of patients is recorded in Table1.Table 1
General patient information.
ProjectSS group (n=39)ZnO-BC/PVA group (n=42)SexMale2324Female1618Age (years)47.52±5.6748.29±4.82Course of disease (years)1.68±0.791.74±0.83Tumor diameter (mm)3.21±0.873.19±0.74Disease siteLeft1921Right2021


### 2.4.2. Inclusion and Exclusion Criteria

The inclusion criteria for this work are as follows: the patient’s cognitive function is normal, and there is no redness, swelling, or purulent discharge on the wound after adrenalectomy; the patient and his family are professionally trained and qualified for home treatment; the study adopts the principle of patient voluntariness; all patients have signed informed consent. The exclusion criteria include patients with a history of infection in the recent (1.0–3 months) and other clinical trials; those with discomfort or allergies to ZnO NP-based antibacterial dressings; patients with critical illness; and the inability to evaluate drugs due to the use of other drugs. Effective patients can withdraw from this study when they have serious adverse reactions.


### 2.4.3. Nursing Plan

Disinfect the wounds of the two groups of patients, implement the corresponding medical antibacterial dressing treatment, and guide the patients to change the dressing operation (Figure1). In addition, pay close attention to the patient’s psychological state and wound healing, and provide timely guidance. Provide scientific guidance on diet, and urge patients to strengthen nutrition and eat high-calcium, high-vitamin, and high-protein foods to speed up wound healing. Record the bleeding, exudation, pain, and other conditions of the wounds of the two groups of patients, and take targeted treatment measures. For instance, when replacing the antibacterial dressing, use 0.50% povidone-iodine and normal saline to treat the wound repeatedly before changing the dressing. The frequency of dressing change between the two groups of patients was adjusted according to the severity of exudation and pain, and wound secretions were collected regularly for in vitro culture to identify bacteria accumulated on the wound and evaluate wound healing.Figure 1
Application of ZnO-BC/PVA antibacterial nursing dressing in nursing after adrenalectomy.


### 2.4.4. Measurement Index

After three weeks of antibacterial dressing treatment, the total effective rate, VSA score and nursing satisfaction of the two groups of patients were counted. Among them, the wound is fully healed and no scar is counted as healed; the wound with a reduction of 30.0% and a smaller scar is counted as significant; the wound with a reduction of 50% and scars is counted as effective; and the wound with a smaller reduction rate is counted as ineffective. The timeliness of the two dressings was evaluated by counting the number of dressing changes and the interval between patients, and the number of bacteria on the wounds of the two groups of patients was detected to evaluate the antibacterial properties of the dressings. Statistical analysis was made on the adverse reactions and complication rates of the two medical antibacterial dressings.


## 2.4.1. General Patient Information

Eighty-one patients undergoing adrenalectomy were selected, including 41 males and 40 females. The age distribution ranged from 31 to 62 years, and the disease course was in the distribution range of one to three years. The patients were randomly divided into two groups, namely, silver sulfadiazine (SS) and ZnO NP-based antibacterial dressing group (ZnO-BC/PVA). And the baseline data of each group of patients is recorded in Table1.Table 1
General patient information.
ProjectSS group (n=39)ZnO-BC/PVA group (n=42)SexMale2324Female1618Age (years)47.52±5.6748.29±4.82Course of disease (years)1.68±0.791.74±0.83Tumor diameter (mm)3.21±0.873.19±0.74Disease siteLeft1921Right2021


## 2.4.2. Inclusion and Exclusion Criteria

The inclusion criteria for this work are as follows: the patient’s cognitive function is normal, and there is no redness, swelling, or purulent discharge on the wound after adrenalectomy; the patient and his family are professionally trained and qualified for home treatment; the study adopts the principle of patient voluntariness; all patients have signed informed consent. The exclusion criteria include patients with a history of infection in the recent (1.0–3 months) and other clinical trials; those with discomfort or allergies to ZnO NP-based antibacterial dressings; patients with critical illness; and the inability to evaluate drugs due to the use of other drugs. Effective patients can withdraw from this study when they have serious adverse reactions.


## 2.4.3. Nursing Plan

Disinfect the wounds of the two groups of patients, implement the corresponding medical antibacterial dressing treatment, and guide the patients to change the dressing operation (Figure1). In addition, pay close attention to the patient’s psychological state and wound healing, and provide timely guidance. Provide scientific guidance on diet, and urge patients to strengthen nutrition and eat high-calcium, high-vitamin, and high-protein foods to speed up wound healing. Record the bleeding, exudation, pain, and other conditions of the wounds of the two groups of patients, and take targeted treatment measures. For instance, when replacing the antibacterial dressing, use 0.50% povidone-iodine and normal saline to treat the wound repeatedly before changing the dressing. The frequency of dressing change between the two groups of patients was adjusted according to the severity of exudation and pain, and wound secretions were collected regularly for in vitro culture to identify bacteria accumulated on the wound and evaluate wound healing.Figure 1
Application of ZnO-BC/PVA antibacterial nursing dressing in nursing after adrenalectomy.


## 2.4.4. Measurement Index

After three weeks of antibacterial dressing treatment, the total effective rate, VSA score and nursing satisfaction of the two groups of patients were counted. Among them, the wound is fully healed and no scar is counted as healed; the wound with a reduction of 30.0% and a smaller scar is counted as significant; the wound with a reduction of 50% and scars is counted as effective; and the wound with a smaller reduction rate is counted as ineffective. The timeliness of the two dressings was evaluated by counting the number of dressing changes and the interval between patients, and the number of bacteria on the wounds of the two groups of patients was detected to evaluate the antibacterial properties of the dressings. Statistical analysis was made on the adverse reactions and complication rates of the two medical antibacterial dressings.


## 2.5. Statistical Methods

Use SPSS 22.0 to process, and analyze the data involved in this research. Quantitative variables are expressed as themean±standarddeviationx¯±s, and qualitative variables are described by frequency distribution and percentage (%). The t-test is used for the analysis of quantitative variables, and the analysis of qualitative variables uses the χ2 test. P<0.05 indicates statistical significance.


## 3. Results and Discussion

### 3.1. Morphological Characteristics of ZnO-BC/PVA

SEM, as a promising characterization technology for material morphology research, can provide important information such as the shape and particle size of the material. The SEM morphology image of ZnO-BC/PVA is shown in Figure2. On the one hand, adding BC to the PVA gel can form very fine pores at the communication between the two structures. And with the incorporation of ZnO, the porosity further increases. On the other hand, lamellar and ravine cross-sections can be observed on the ZnO-BC/PVA gel film. The addition of BC reduces the original three-dimensional polymer morphology of PVA, and the appearance of holes, cracks, and ravines appears. This result leads to a decrease in the degree of entanglement of PVA itself, resulting in an increase in the size of the relative entangled porous, thus breaking the original three-dimensional polymer morphology of PVA. From the PVA/BC cross-section, it is observed that the white light-emitting points are ZnO nanoparticles, which are firmly incorporated in the PVA/BC matrix. According to the statistics of the distribution of ZnO nanoparticles, it is found that the distribution of ZnO nanoparticles in the PVA/BC matrix is relatively uniform. The increased ZnO nanoparticles reduce the effect of BC-induced PVA pore enlargement, which is attributed to the van der Waals force between ZnO nanoparticles and BC, which can make ZnO-BC/PVA to pack tightly and reduce the system ability.Figure 2
Characterization of the morphology of zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA).


### 3.2. Pain Assessment and VAS Score

We aim to further clarify the effect of ZnO-BC/PVA antibacterial dressing in the actual experience of patients and to investigate whether it has analgesic effect on patients’ wounds. By applying the antibacterial dressing ZnO-BC/PVA to the surgical wounds of patients undergoing PLA and at the same time recording the changes in the patient’s wound pain index before and 1, 2, and 3 weeks after the care of the patient, it is used to evaluate the analgesic effect of the new antibacterial material. The results are displayed in Figure3. The pain scoring standard is assessed by the visual analog scale (VAS), where zero points represents no pain and ten points represents severe pain. Statistical analysis shows that patients who use the new ZnO-BC/PVA antibacterial dressing and those who use traditional silver sulfadiazine have similar low pain, which further shows that ZnO-BC/PVA can reduce the postoperative wound surface while relieving antibacterial pain.Figure 3
Comparison of the analgesic ability of zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).


### 3.3. Wound Healing Rate

Figure4 shows the effect of ZnO-BC/PVA antibacterial dressing on wound healing after three weeks. Statistics found that the healing rate of patients in the ZnO-BC/PVA group (16.67%) was significantly higher than that of the SS group (7.69%). In addition, the number of invalid patients is small, which proves that compared with the SS group, ZnO-BC/PVA antibacterial dressing has a better nursing effect after adrenalectomy.Figure 4
Comparison of the wound healing ability of zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).
(a)(b)


### 3.4. Interval between Dressing Changes

After the dressing is used for a period of time, a new dressing needs to be replaced. However, frequent dressing changes will expose the wound to the air, which will increase the number of bacteria on the wound. In order to prevent wound infections in patients, the number of dressing changes in the nursing process should be minimized. Increase the interval between dressing changes. Figure5 shows the average time between dressing changes for patients in three weeks. Statistical analysis showed that 48.0% of the patients in the ZnO-BC/PVA group had a dressing change interval of more than 72 h. Compared with the SS group, the dressing change interval was significantly increased (P<0.05).Figure 5
Comparison of dressing change intervals between zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).


### 3.5. Bacteria Detection Rate on Wound

In order to further research the anti-infection ability of ZnO-BC/PVA dressings, the detection rate of bacteria on the wound surface was tested every three days, and the results are recorded in Figure6. With the increase of time, the detection rate of bacteria on the wounds of patients showed a declining trend. And compared with the SS group, the detection rate of ZnO-BC/PVA bacteria decreased faster, and only 4.36±1.23 was detected at 21 days, while in the SS group it was 26.81±1.54%. This result proves that ZnO-BC/PVA dressing can significantly reduce the bacterial content of wounds and reduce the possibility of patient infection.Figure 6
Comparison of the detection rate of wound bacteria between zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).


### 3.6. Adverse Reaction Rate

Wound infection, bleeding, shock, and other adverse reactions often occur in nursing after adrenalectomy. Figure7 shows the occurrence of adverse reactions during the nursing process of the ZnO-BC/PVA group and the SS group. According to statistical analysis, the incidence of adverse reactions in the ZnO-BC/PVA group was low, and there were no serious adverse reactions such as shock. It shows that ZnO-BC/PVA can significantly reduce the occurrence of adverse reactions in postoperative care.Figure 7
The incidence of adverse reactions between zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS). (a) ZnO-BC/PVA. (b) SS.
(a)(b)


## 3.1. Morphological Characteristics of ZnO-BC/PVA

SEM, as a promising characterization technology for material morphology research, can provide important information such as the shape and particle size of the material. The SEM morphology image of ZnO-BC/PVA is shown in Figure2. On the one hand, adding BC to the PVA gel can form very fine pores at the communication between the two structures. And with the incorporation of ZnO, the porosity further increases. On the other hand, lamellar and ravine cross-sections can be observed on the ZnO-BC/PVA gel film. The addition of BC reduces the original three-dimensional polymer morphology of PVA, and the appearance of holes, cracks, and ravines appears. This result leads to a decrease in the degree of entanglement of PVA itself, resulting in an increase in the size of the relative entangled porous, thus breaking the original three-dimensional polymer morphology of PVA. From the PVA/BC cross-section, it is observed that the white light-emitting points are ZnO nanoparticles, which are firmly incorporated in the PVA/BC matrix. According to the statistics of the distribution of ZnO nanoparticles, it is found that the distribution of ZnO nanoparticles in the PVA/BC matrix is relatively uniform. The increased ZnO nanoparticles reduce the effect of BC-induced PVA pore enlargement, which is attributed to the van der Waals force between ZnO nanoparticles and BC, which can make ZnO-BC/PVA to pack tightly and reduce the system ability.Figure 2
Characterization of the morphology of zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA).


## 3.2. Pain Assessment and VAS Score

We aim to further clarify the effect of ZnO-BC/PVA antibacterial dressing in the actual experience of patients and to investigate whether it has analgesic effect on patients’ wounds. By applying the antibacterial dressing ZnO-BC/PVA to the surgical wounds of patients undergoing PLA and at the same time recording the changes in the patient’s wound pain index before and 1, 2, and 3 weeks after the care of the patient, it is used to evaluate the analgesic effect of the new antibacterial material. The results are displayed in Figure3. The pain scoring standard is assessed by the visual analog scale (VAS), where zero points represents no pain and ten points represents severe pain. Statistical analysis shows that patients who use the new ZnO-BC/PVA antibacterial dressing and those who use traditional silver sulfadiazine have similar low pain, which further shows that ZnO-BC/PVA can reduce the postoperative wound surface while relieving antibacterial pain.Figure 3
Comparison of the analgesic ability of zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).


## 3.3. Wound Healing Rate

Figure4 shows the effect of ZnO-BC/PVA antibacterial dressing on wound healing after three weeks. Statistics found that the healing rate of patients in the ZnO-BC/PVA group (16.67%) was significantly higher than that of the SS group (7.69%). In addition, the number of invalid patients is small, which proves that compared with the SS group, ZnO-BC/PVA antibacterial dressing has a better nursing effect after adrenalectomy.Figure 4
Comparison of the wound healing ability of zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).
(a)(b)


## 3.4. Interval between Dressing Changes

After the dressing is used for a period of time, a new dressing needs to be replaced. However, frequent dressing changes will expose the wound to the air, which will increase the number of bacteria on the wound. In order to prevent wound infections in patients, the number of dressing changes in the nursing process should be minimized. Increase the interval between dressing changes. Figure5 shows the average time between dressing changes for patients in three weeks. Statistical analysis showed that 48.0% of the patients in the ZnO-BC/PVA group had a dressing change interval of more than 72 h. Compared with the SS group, the dressing change interval was significantly increased (P<0.05).Figure 5
Comparison of dressing change intervals between zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).


## 3.5. Bacteria Detection Rate on Wound

In order to further research the anti-infection ability of ZnO-BC/PVA dressings, the detection rate of bacteria on the wound surface was tested every three days, and the results are recorded in Figure6. With the increase of time, the detection rate of bacteria on the wounds of patients showed a declining trend. And compared with the SS group, the detection rate of ZnO-BC/PVA bacteria decreased faster, and only 4.36±1.23 was detected at 21 days, while in the SS group it was 26.81±1.54%. This result proves that ZnO-BC/PVA dressing can significantly reduce the bacterial content of wounds and reduce the possibility of patient infection.Figure 6
Comparison of the detection rate of wound bacteria between zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS).


## 3.6. Adverse Reaction Rate

Wound infection, bleeding, shock, and other adverse reactions often occur in nursing after adrenalectomy. Figure7 shows the occurrence of adverse reactions during the nursing process of the ZnO-BC/PVA group and the SS group. According to statistical analysis, the incidence of adverse reactions in the ZnO-BC/PVA group was low, and there were no serious adverse reactions such as shock. It shows that ZnO-BC/PVA can significantly reduce the occurrence of adverse reactions in postoperative care.Figure 7
The incidence of adverse reactions between zinc oxide-bacterial cellulose/polyvinyl alcohol (ZnO-BC/PVA) and silver sulfadiazine (SS). (a) ZnO-BC/PVA. (b) SS.
(a)(b)


## 4. Discussion

RLA has been widely used in clinical practice, but it is prone to wound infection, bleeding, and even shock during postoperative care [22]. Dressings are considered to be an important method to promote wound healing and prevent bacterial infections [23]. The ideal dressing should have multiple properties such as flexibility, porosity, anti-infection, and high swelling properties [24]. PVA is a water-soluble polymer with a simple chemical structure and can be chemically modified through some simple reactions [25, 26]. Meanwhile, PVA gel also has a high degree of swelling, which is a good dressing carrier [27]. In addition, ZnO NPs, as a safe and nontoxic metal oxide nanomaterial, are widely used in the biomedical field because of their antibacterial, antioxidant, and anticancer properties [28, 29]. Based on the antibacterial activity of ZnO NPs, Bharathi and Bhuvaneshwari [30] developed a safer and more environmentally friendly synthesis method for ZnO NPs. At the same time, they proved that ZnO NPs have good antibacterial, antioxidant, and antibreast cancer cell proliferation effects, and can be used as a powerful bactericide.In summary, this work prepared a new long-acting antibacterial and breathable wound dressing ZnO-BC/PVA based on ZnO NPs. The characterization results showed that the ZnO-BC/PVA antibacterial dressing has a compact porous structure. The particles are relatively evenly distributed in the PVA/BC matrix, which is suitable for clinical application as a dressing. In clinical trials, the ZnO-BC/PVA dressing showed good antibacterial properties, and its anti-infection ability was stronger than traditional dressings. At the same time, in the 21-day treatment, ZnO-BC/PVA dressing can effectively promote wound healing, extend the interval between dressing changes, reduce the pain of the patient, and significantly reduce the incidence of postoperative complications. Therefore, the ZnO-BC/PVA material synthesized in this research can be used as an antibacterial dressing in clinical nursing after adrenalectomy.


## 5. Conclusion

The study contributed a ZnO-BC/PVA antibacterial gel dressing for wound care after adrenalectomy was constructed based on BC and PVA polymer composite network coated with ZnO NPs. It possesses antibacterial, analgesic, and anti-infection properties. It can also speed up wound healing and decrease problems. This study offers a new clinical alternative following adrenalectomy.



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*Source: 9051927-2022-03-07.xml*