# Ficus deltoidea Jack: A Review on Its Phytochemical and Pharmacological Importance

Authors: Hamidun Bunawan; Noriha Mat Amin; Siti Noraini Bunawan; Syarul Nataqain Baharum; Normah Mohd Noor
Journal: Evidence-Based Complementary and Alternative Medicine
 (2014)

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## Abstract

Ficus deltoidea Jack (Moraceae) has had a long history of use in traditional medicine among the Malays to alleviate and heal ailments such as sores, wounds, and rheumatism and as an after-birth tonic and an antidiabetic drug. Modern pharmacological studies demonstrated that this plant has a wide variety of beneficial attributes for human health. Despite its importance, a review of this species has not been published in the scientific literature to date. Here, we review and summarize the historic and current literature concerning the botany, traditional uses, phytochemistry, pharmacological effects, and toxicity of this wonder plant. This summary could be beneficial for future research aiming to exploit the therapeutic potential of this useful, medicinal species.


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## Body

## 1. Introduction

For many years there has been a tremendous interest in biological products gained from megadiversity countries like Malaysia. These invaluable products are explored for the potential discovery of novel biomolecules that have possible future uses. Recently, natural plant products have been gaining popularity in the prevention and treatment of various diseases. Traditional medicines from plants have attracted major attention worldwide because of their potential pharmaceutical importance.One of the most popular and well-known plants with a long history of use among the Malays isFicus deltoideaJack, a plant of the family Moraceae.Ficus deltoidea not only has been used as a medicine for various ailments in the Malay Archipelago, but also has been distributed and formulated as capsules, tea, and tonic tea throughout Malaysia. This review will unify fragmented information regardingF. deltoidea in terms of its botany, traditional uses, phytochemistry, and pharmaceutical effects in order to facilitate better understanding and provide further support for the ethnopharmacological use of this important species.


## 2. Botany

### 2.1. Botanical Name

Its botanical name isFicus deltoidea Jack.


#### 2.1.1.  Subsp.
Deltoidea

The synonyms areF.deltoidea var.angustifolia (Miq.) Corner,F.deltoidea formaangustissima Corner,F.deltoidea var.arenaria Corner,F.deltoidea var.bilobata Corner,F.deltoidea var.borneensisCorner,F.deltoidea formasubhirsuta Corner,F.deltoidea var.kunstleri (King) Corner,F.deltoidea var.lutescens (Desf.) Corner,F.deltoidea formalongipedunculata Corner,F.deltoidea formasubsessilis (Miq.) Corner,F.deltoidea var.peltata Corner,F.deltoidea var.recurvata Kochummen, andF.deltoidea var.trengganuensis Corner [8].


#### 2.1.2. Subsp.motleyana (Miq.) C.C Berg, Comb. and Stat. Nov

The synonym isF. deltoidea var.oligoneura (Miq.) Corner [8].


### 2.2. Common Names

It is commonly known as mistletoe fig and also as Mas Cotek, Telinga Beruk, or Serapat Angin by Malays. Other vernacular names include Sempit-Sempit and Agoluran for people in Sabah, Sarawak, and the Kalimantan Islands, Tabat Barito in Indonesia, and Kangkalibang in Africa.


### 2.3. Botanical Description and Distribution

Ficus deltoidea is an evergreen shrub that reaches a height of 2 meters, with whitish grey bark, leaves broadly spoon-shaped to obovate, with a leaf length between 4 cm and 8 cm, coloured bright green above, and rust-red to olive-brown beneath. The plant produces figs that are spherical to round, with a width of 1.0 to 1.5 cm and coloured yellow to orange-red (Figure 1). This plant is native to the Malayan Archipelago and has been introduced elsewhere. It is distributed throughout the region known as Malesia, which includes Thailand, Indonesia, and Malaysia [9].Figure 1
Ficus deltoidea commonly known as Mas Cotek in Malaysia.


## 2.1. Botanical Name

Its botanical name isFicus deltoidea Jack.


### 2.1.1.  Subsp.
Deltoidea

The synonyms areF.deltoidea var.angustifolia (Miq.) Corner,F.deltoidea formaangustissima Corner,F.deltoidea var.arenaria Corner,F.deltoidea var.bilobata Corner,F.deltoidea var.borneensisCorner,F.deltoidea formasubhirsuta Corner,F.deltoidea var.kunstleri (King) Corner,F.deltoidea var.lutescens (Desf.) Corner,F.deltoidea formalongipedunculata Corner,F.deltoidea formasubsessilis (Miq.) Corner,F.deltoidea var.peltata Corner,F.deltoidea var.recurvata Kochummen, andF.deltoidea var.trengganuensis Corner [8].


### 2.1.2. Subsp.motleyana (Miq.) C.C Berg, Comb. and Stat. Nov

The synonym isF. deltoidea var.oligoneura (Miq.) Corner [8].


## 2.1.1.  Subsp.
Deltoidea

The synonyms areF.deltoidea var.angustifolia (Miq.) Corner,F.deltoidea formaangustissima Corner,F.deltoidea var.arenaria Corner,F.deltoidea var.bilobata Corner,F.deltoidea var.borneensisCorner,F.deltoidea formasubhirsuta Corner,F.deltoidea var.kunstleri (King) Corner,F.deltoidea var.lutescens (Desf.) Corner,F.deltoidea formalongipedunculata Corner,F.deltoidea formasubsessilis (Miq.) Corner,F.deltoidea var.peltata Corner,F.deltoidea var.recurvata Kochummen, andF.deltoidea var.trengganuensis Corner [8].


## 2.1.2. Subsp.motleyana (Miq.) C.C Berg, Comb. and Stat. Nov

The synonym isF. deltoidea var.oligoneura (Miq.) Corner [8].


## 2.2. Common Names

It is commonly known as mistletoe fig and also as Mas Cotek, Telinga Beruk, or Serapat Angin by Malays. Other vernacular names include Sempit-Sempit and Agoluran for people in Sabah, Sarawak, and the Kalimantan Islands, Tabat Barito in Indonesia, and Kangkalibang in Africa.


## 2.3. Botanical Description and Distribution

Ficus deltoidea is an evergreen shrub that reaches a height of 2 meters, with whitish grey bark, leaves broadly spoon-shaped to obovate, with a leaf length between 4 cm and 8 cm, coloured bright green above, and rust-red to olive-brown beneath. The plant produces figs that are spherical to round, with a width of 1.0 to 1.5 cm and coloured yellow to orange-red (Figure 1). This plant is native to the Malayan Archipelago and has been introduced elsewhere. It is distributed throughout the region known as Malesia, which includes Thailand, Indonesia, and Malaysia [9].Figure 1
Ficus deltoidea commonly known as Mas Cotek in Malaysia.


## 3. Ethnobotanical Uses

Malays in the Peninsular Malaysia have been using the powdered root and leaves ofF. deltoidea to treat wounds, rheumatism, sores, and other ailments for centuries. The decoction of boiled leaves ofF. deltoidea is traditionally used as an antidiabetic treatment and an after-birth tonic to contract the uterus and vaginal muscles, to treat disorders of the menstrual cycle, and also to treat leucorrhoea [10]. Additionally,F. deltoidea fruits are traditionally chewed to relieve toothache, cold, and headache and the entireFicus deltoidea plant is also traditionally used as an aphrodisiac tonic and as health tonic by women in Indonesia.


## 4. Chemical Composition

A wide range of chemical compounds have been isolated and characterized fromF. deltoidea, particularly from the leaves and figs. An initial comprehensive study on volatile compounds produced by the fruits was conducted by Grison-Pigé et al. [1, 2]. The volatile compounds isolated and identified are mainly products of the shikimic acid pathway, terpenoids, and aliphatic groups, generally present as floral fragrances in plants. In 2009, Mohd Lip et al. [5] isolated and identified moretenol fromF. deltoidea leaves using nuclear magnetic resonance (NMR) and mass spectrometers. Later on, an antibacterial compound known as lupeol (C30H50O) was also isolated from the leaves ofF. deltoidea and exhibited toxicity againstStaphylococcus aureus,Bacillus subtilis,andEscherichia coli [6]. Ong et al. [7] demonstrated enhancement of flavonoid compounds (rutin, quercetin, and naringenin) in cell cultures ofF. deltoidea influenced by different carbon sources as well as plant growth regulators. A comprehensive study on flavonoid compounds of aqueous extract ofF. deltoidea was conducted by Omar et al. [3] using HPLC-MS-based methodology and more than 25 compounds were identified. Currently, two bioactive constituents known as vitexin and isovitexin have been isolated, identified, and evaluated to show α-glucosidase inhibition [4]. The metabolites identified fromF. deltoidea to date are presented in Table 1.Table 1
Chemical constituents identified inF. deltoidea.
Plant part used
Compound
Class
References
Fig
4-Methylbenzaldehyde
Shikimic acid
[1]
Methyl benzoate
Shikimic acid
[1]
Indole
Shikimic acid
[1]
Decanal
Aliphatics
[1]
6-Methyl-5-hepten-2-one
Acyclic monoterpenes
[1]
Myrcene
Acyclic monoterpenes
[1]
(Z)-β-Ocimene
Acyclic monoterpenes
[1]
(E)-β-Ocimene
Acyclic monoterpenes
[1]
cis-Furanoid linalool oxide
Acyclic monoterpenes
[1]
trans-Furanoid linalool oxide
Acyclic monoterpenes
[1, 2]
Linalool
Acyclic monoterpenes
[1, 2]
cis-Pyranoid linalool oxide
Acyclic monoterpenes
[1]
trans-Pyranoid linalool oxide
Acyclic monoterpenes
[1]
Hotrienol
Acyclic monoterpenes
[1]
Perillene
Acyclic monoterpenes
[1]
Limonene
Cyclic monoterpenes
[1]
Dendrolasine
Sesquiterpenes
[1]
α-Cubebene
Sesquiterpenes
[1]
Cyclosativene
Sesquiterpenes
[1]
Α-Ylangene
Sesquiterpenes
[1]
α-Copaene
Sesquiterpenes
[1, 2]
β-Bourbonene
Sesquiterpenes
[1]
1,5-Diepi-β-bourbonene
Sesquiterpenes
[1]
β-Cubebene
Sesquiterpenes
[1, 2]
β-Elemene
Sesquiterpenes
[1]
α-Gurjunene
Sesquiterpenes
[1]
α-cis-Bergamotene
Sesquiterpenes
[1]
β-Caryophyllene
Sesquiterpenes
[1, 2]
α-Santalene
Sesquiterpenes
[1]
Selina-3-6-diene
Sesquiterpenes
[1]
α-trans-Bergamotene
Sesquiterpenes
[1, 2]
α-Humulene
Sesquiterpenes
[1, 2]
Alloaromadendrene
Sesquiterpenes
[1, 2]
Aciphyllene
Sesquiterpenes
[1]
Germacrene D
Sesquiterpenes
[1, 2]
β-Selinene
Sesquiterpenes
[1]
αd-Selinene
Sesquiterpenes
[1]
α-Selinene
Sesquiterpenes
[1]
Bicyclogermacrene
Sesquiterpenes
[1]
α-Muurolene
Sesquiterpenes
[1]
Germacrene A
Sesquiterpenes
[1, 2]
δ-Amorphene
Sesquiterpenes
[1]
(E,E)α-Farnesene
Sesquiterpenes
[1, 2]
2-epi-α-Selinene
Sesquiterpenes
[1]
δ-Cadinene
Sesquiterpenes
[1, 2]
Cadina-1,4-diene
Sesquiterpenes
[1]
Germacrene B
Sesquiterpenes
[1]
Caryophyllene oxide
Sesquiterpenes
[1]
Leaves
Gallocatechin
Flavonoids
[3]
Epigallocatechin
Flavonoids
[3]
Catechin
Flavonoids
[3]
(Epi)afzelechin-(epi)catechin
Flavonoids
[3]
(Epi)afzelechin-(epi)afzelechin
Flavonoids
[3]
(Epi)catechin Epicatechin
Flavonoids
[3]
Luteolin-6,8-C-diglucoside (lucenin-2)
Flavonoids
[3]
Apigenin-6,8-C-diglucoside (vicenin-2)
Flavonoids
[3]
Luteolin-6-C-hexosyl-8-C-pentoside
Flavonoids
[3]
Luteolin-6-C-glucosyl-8-C-arabinoside
Flavonoids
[3]
Apigenin-6-C-arabinosyl-8-C-glucoside (isoschaftoside)
Flavonoids
[3]
Luteolin-6-C-arabinosyl-8-C-glucoside
Flavonoids
[3]
Apigenin-6-C-glucoside-8-C-arabinoside (schaftoside)
Flavonoids
[3]
Luteolin-8-C-glucoside (orientin)
Flavonoids
[3]
Apigenin-6-C-pentosyl-8-C-glucoside
Flavonoids
[3]
Apigenin-8-C-glucoside (vitexin)
Flavonoids
[3, 4]
Apigenin-6-C-glucosyl-8-C-pentoside
Flavonoids
[3]
Apigenin-6,8-C-dipentoside isomer
Flavonoids
[3]
Apigenin-6-C-glucoside (isovitexin)
Flavonoids
[3, 4]
4-p-coumarolyquinic acid
Flavonoids
[3]
Moretenol
Terpenes
[5]
(3β,13ξ)-Lup-20(29)-en-3-ol (lupeol)
Triterpenes
[6]
Cell culture
Rutin
Flavonoids
[7]
Quercetin
Flavonoids
[7]
Naringenin
Flavonoids
[7]


## 5. Pharmacological Properties

### 5.1. Antidiabetic

Ficus deltoidea is traditionally used in Malaysia to control blood sugar levels. Adam et al. [11] evaluated the potential of five extracts and three fractions ofF. deltoidea in order to enhance basal and insulin-stimulated glucose uptake into the Chang liver cell line. The results show thatF. deltoidea extracts and fractions, with the exception of petroleum ether, possess the potential to stimulate basal and insulin-stimulated glucose uptake into the liver cell line, with ethanolic extract having the highest insulin mimetic activity. Another study by Adam et al. [12] reported that hot aqueous, ethanolic, and methanolic extracts ofF. deltoidea inhibit rat intestine α-glucosidase activity significantly, an enzyme that is accountable for carbohydrate digestion to condense the effect of carbohydrate in blood sugar. This was supported in an animal model in which all extracts reduced postprandial hyperglycemia after sucrose administration. Methanolic extract was found to be the most potent inhibitor of α-glucosidase [12].Another study by Farsi et al. [13] reported that the n-butanol fraction from the leaves ofF. deltoidea showed visible α-glucosidase and α-amylase inhibitory effects to control postprandial hyperglycemia. A different study conducted by Adam et al. [14] to evaluate the ethanolic effect ofF. deltoidea on glucose levels in normal rats showed that all doses reduced fasting blood glucose, especially after 6 hours of administration. Adam et al. [15] also evaluated extracts ofF. deltoidea for hyperglycemia effects at different prandial states. The study found that hot aqueous extracts ofF. deltoidea stimulate insulin secretion and show a high magnitude of stimulation and the extract induced the usage of intracellular Ca2+ to initiate release of insulin, whilst increased basal and insulin-mediated glucose uptake into adipocytes cells was observed with ethanolic and methanolic extracts.Partitioned extracts, subfractions, and pure bioactive constituents were the subject of anα-glucosidase inhibition assay. Both vitexin and isovitexin significantly reduced postprandial blood glucose level in mice, were nontoxic even at the highest dose of 2 g per kg, and exhibitedin vivo  α-glucosidase activity (Figure 2) [4]. Draman et al. [16] evaluated the effects ofF. deltoidea leaves on fasting blood sugar and renal and lipid profiles of type 2 diabetic humans in Malaysia. The patients were givenF. deltoidea 350 mg orally twice daily for two months. Even though the patients reported feeling energetic and fresh, effects on fasting blood sugar, HbA1C, and renal and lipid profiles were not significant. On the other hand, Kalman et al. [17] reported thatF. deltoidea has significant effect on reducing glucose and lipid levels in human body after testing the antidiabetic effects ofF. deltoideain adults with prediabetes.(a) Vitexin and (b) isovitexin chemical structures.
(a)
(b)Recently, the fruits of two varieties ofF. deltoidea, var.angustifolia and var.kunstleri, were separately extracted and the antidiabetic activities were evaluated according to the ability of the extract to inhibit both yeast and mammalian α-glucosidase and α-amylase [18]. The study also found that the crude extracts and fractions of both varieties hindered yeast and rat intestinal α-glucosidases depending on dose used but failed to inhibit porcine pancreatic α-amylase.


### 5.2. Anti-Inflammatory and Antinociceptive Activity

The anti-inflammatory activity of the aqueous extract ofF. deltoidea leaf has been evaluated in rats using a carrageenan-induced paw edema test, a cotton pellet-induced granuloma test, and a formalin test, demonstrating thatF. deltoidea had significant anti-inflammatory activity in every test with dose-response effects observed [19]. The leaves possess anti-inflammatory effects against various inflammatory responses as well as pain-associated inflammatory responses. Previously, Abdullah et al. [20] evaluated standardised extracts of different varieties ofF. deltoidea for anti-inflammatory activity using threein vitro assays: lipoxygenase, hyaluronidase, and TPA-induced edema. The results demonstrated that extracts of leaves ofF. deltoidea have significant anti-inflammatory properties, suggesting that the extracts may be useful for relieving pain by reducing inflammation.Antinociceptive activity ofF. deltoidea aqueous extract from leaves was studied by Sulaiman et al. [21] in order to verify using this plant to reduce sensitivity to painful stimuli using chemical and thermal nociception models. Three methods were used in their experiment: acetic acid-induced abdominal writhing, formalin, and a hot plate test. Significant antinociceptive effects ofF. deltoidea leaves were observed in all three methods used when administered 30 minutes prior to pain induction. The results demonstrate thatF. deltoidea leaves do have antinociceptive activity, corroborating its traditional use in treating painful conditions, such as headache and toothache.


### 5.3. Antimelanogenic and Antiphotoaging Effect

Ficus deltoidea was also reported to have antimelanogenic effects in reducing melanogenesis biosynthesis for melanin production [22].Ficus deltoidea extract activity was investigated using cultured B16F1 melanoma cells focusing on the characterization of tyrosinase, the enzyme that is required for melanin production. The extract fromF. deltoidea inhibited mushroom tyrosinase activity and intracellular tyrosinase activity of B16F1. Microphthalmia-associated transcription factor (MITF) expression also decreased whenF. deltoidea extract was applied, suggesting thatF. deltoidea has strong potential for use as a depigmenting agent in cosmetics.Treatment withF. deltoidea extract also significantly inhibited UV-induced TNF-α, IL-1α, IL-6, and COX-2 expression [23]. Decreased collagen synthesis of fibroblasts as a result of UVB exposure returned to normal afterF. deltoidea extract treatment. Furthermore, following UVB irradiation enhanced MMP-1 expression that would usually be observed decreased after treatment with theF. deltoidea extract in a dose-dependent manner. The study concluded that theF. deltoidea extract may have a positive effect against UVB-induced skin damage and that it is useful for antiphotoaging cosmetic products [23].


### 5.4. Antioxidant Effect

The antioxidant property of theF. deltoidea extract was revealed through a total phenolic content and ferric reducing antioxidant potential (FRAP) assay by Omar et al. [3]. It was found that flavan-3-ol monomers and proanthocyanidins contributed 85% of the antioxidant activity of the aqueous extract ofF. deltoidea (Figure 3). In contrast, this figure was 15% for flavones, primarily due to apigenin-6,8-C-diglucoside. Previously, Hakiman and Maziah [24] described another experiment where different aqueous extracts ofF. deltoidea accessions were evaluated for their antioxidant activities using several assays such as FRAP, free radical scavenging assay, total polyphenol, flavonoid, phenolic acid, and vitamin C.Chemical structure of (a) flavan-3-ol monomer and (b) proanthocyanidins.
(a)
(b)


### 5.5. Antiulcerogenic Effect

The potential ofF. deltoidea whole-plant extract as an ulcer healing agent for gastric ulcers caused by ethanol in Sprague Dawley rats was investigated by Zahra et al. [25]. Four groups of rats were used in the experiment: negative controls (treated with distilled water), 250 and 500 mg kg−1 ofF. deltoidea extract, and positive controls (treated with omeprazole). Pretreatment withF. deltoidea extract demonstrated considerably less gastric mucosal lesions produced by ulcerogens compared to the negative control. Predictably, gastric protection was more effective with use of 500 mg kg−1  F. deltoidea extract compared to 250 mg kg−1. The negative control rats showed very severe gastric mucosal damage as well as edema and leucocyte permeation of the submucosal layer, suggesting thatF. deltoidea extract protects against ulcers, demonstrated by the reduction in ulcer areas, inhibition of submucosal edema, and reduced leucocyte infiltration.


### 5.6. Wound Healing Activity

The effects ofF. deltoidea extract for healing of wounds in Sprague Dawley rats were evaluated by Abdulla et al. [26]. The group that was treated with a placebo containing 5% and 10% ofF. deltoidea extracts showed significantly accelerated wound healing in comparison with deionized water (negative control) and blank placebo treatments. The rats treated withF. deltoidea also showed decreased scar width, more fibroblast proliferation, and more collagen fibres accompanied by angiogenesis in the granulation tissue.


### 5.7. Antibacterial Activity

A study by Uyub et al. [27] found thatF. deltoidealeaf extract has antimicrobial activity towardsHelicobacter pylori, the major agent in chronic gastritis and gastric ulcers. Methanol extracts ofF. deltoidea showed highest activity againstH. pylori with an inhibition zone diameter of 12.0
±
0.6 mm followed by petroleum ether 10.0
±
0.6 mm and chloroform extract 8.0
±
0.1 mm. Another study found that ethanol and methanol extracts ofF. deltoidea leaves inhibited growth ofBacillus subtilis[28]. This was further supported by Samah et al. [29] who found methanol extracts of wholeF. deltoidea exhibited antibacterial activity againstStaphylococcus aureus,Bacillus subtilis,Escherichia coli, andPseudomonas aeruginosa and antifungal activity towardsCandida albicans.


### 5.8. Anticancer Effect

Aqueous and ethanol extracts ofF. deltoidea were found to have anticancer activity against human ovarian carcinoma cell line A2780 using a cell-based assay and confirmed by microscopic observation [30]. The plant extracts gave IC50 value of 224.39
±
6.24 μg/mL and 143.03
±
20.21 μg/mL in aqueous and ethanolic extracts, respectively. Both of the plant extracts have different effects on cell growth: the aqueous extract promoted cell detachment, while the ethanolic extract decreased cell proliferation. This might be due to different types of phytochemical property in those two extracts. The study also reported that apoptosis was caused by both extracts at 1000 μg/mL.


### 5.9. Miscellaneous Activity

A study was conducted to assess whether the aqueous and ethanolic extracts ofF. deltoidea leaves possessed antithrombotic activity and to observe the effect of the extracts on sperm quality and testosterone levels in diabetic rats [31]. These rats were administered alloxan monohydrate, which is known to cause an increase in blood glucose level, blood clotting rate, and sperm abnormalities and a decrease in testosterone level. Interestingly, rats given aqueous and ethanolic leaf extracts ofF. deltoidea demonstrated significantly increased testosterone levels, sperm count, and motility and showed a reduction in the blood clotting levels and blood glucose level, as well as a reduction in sperm abnormalities.Samsulrizal et al. [32] conducted another similar study with the same result and they additionally considered lactate dehydrogenase C4 (LDH-C4), a specific isoenzyme in mammalian testes that plays a role in sperm capacitation. The effect ofF. deltoidea on LDH-C4 activity was also seen positive for maintaining healthy sperm. A further study on the effects ofF. deltoidea methanolic extract on the reproductive systems of male rats was conducted by Norrizah et al. [33]. Surprisingly, the study found that the leaf extract decreased testes and epididymis weight, sperm count, and sperm viability. Rats treated with stem extract showed a significant increase in epididymis weight and sperm count and viability, although reduction in testes weight and the number of normal sperm morphology was observed.


## 5.1. Antidiabetic

Ficus deltoidea is traditionally used in Malaysia to control blood sugar levels. Adam et al. [11] evaluated the potential of five extracts and three fractions ofF. deltoidea in order to enhance basal and insulin-stimulated glucose uptake into the Chang liver cell line. The results show thatF. deltoidea extracts and fractions, with the exception of petroleum ether, possess the potential to stimulate basal and insulin-stimulated glucose uptake into the liver cell line, with ethanolic extract having the highest insulin mimetic activity. Another study by Adam et al. [12] reported that hot aqueous, ethanolic, and methanolic extracts ofF. deltoidea inhibit rat intestine α-glucosidase activity significantly, an enzyme that is accountable for carbohydrate digestion to condense the effect of carbohydrate in blood sugar. This was supported in an animal model in which all extracts reduced postprandial hyperglycemia after sucrose administration. Methanolic extract was found to be the most potent inhibitor of α-glucosidase [12].Another study by Farsi et al. [13] reported that the n-butanol fraction from the leaves ofF. deltoidea showed visible α-glucosidase and α-amylase inhibitory effects to control postprandial hyperglycemia. A different study conducted by Adam et al. [14] to evaluate the ethanolic effect ofF. deltoidea on glucose levels in normal rats showed that all doses reduced fasting blood glucose, especially after 6 hours of administration. Adam et al. [15] also evaluated extracts ofF. deltoidea for hyperglycemia effects at different prandial states. The study found that hot aqueous extracts ofF. deltoidea stimulate insulin secretion and show a high magnitude of stimulation and the extract induced the usage of intracellular Ca2+ to initiate release of insulin, whilst increased basal and insulin-mediated glucose uptake into adipocytes cells was observed with ethanolic and methanolic extracts.Partitioned extracts, subfractions, and pure bioactive constituents were the subject of anα-glucosidase inhibition assay. Both vitexin and isovitexin significantly reduced postprandial blood glucose level in mice, were nontoxic even at the highest dose of 2 g per kg, and exhibitedin vivo  α-glucosidase activity (Figure 2) [4]. Draman et al. [16] evaluated the effects ofF. deltoidea leaves on fasting blood sugar and renal and lipid profiles of type 2 diabetic humans in Malaysia. The patients were givenF. deltoidea 350 mg orally twice daily for two months. Even though the patients reported feeling energetic and fresh, effects on fasting blood sugar, HbA1C, and renal and lipid profiles were not significant. On the other hand, Kalman et al. [17] reported thatF. deltoidea has significant effect on reducing glucose and lipid levels in human body after testing the antidiabetic effects ofF. deltoideain adults with prediabetes.(a) Vitexin and (b) isovitexin chemical structures.
(a)
(b)Recently, the fruits of two varieties ofF. deltoidea, var.angustifolia and var.kunstleri, were separately extracted and the antidiabetic activities were evaluated according to the ability of the extract to inhibit both yeast and mammalian α-glucosidase and α-amylase [18]. The study also found that the crude extracts and fractions of both varieties hindered yeast and rat intestinal α-glucosidases depending on dose used but failed to inhibit porcine pancreatic α-amylase.


## 5.2. Anti-Inflammatory and Antinociceptive Activity

The anti-inflammatory activity of the aqueous extract ofF. deltoidea leaf has been evaluated in rats using a carrageenan-induced paw edema test, a cotton pellet-induced granuloma test, and a formalin test, demonstrating thatF. deltoidea had significant anti-inflammatory activity in every test with dose-response effects observed [19]. The leaves possess anti-inflammatory effects against various inflammatory responses as well as pain-associated inflammatory responses. Previously, Abdullah et al. [20] evaluated standardised extracts of different varieties ofF. deltoidea for anti-inflammatory activity using threein vitro assays: lipoxygenase, hyaluronidase, and TPA-induced edema. The results demonstrated that extracts of leaves ofF. deltoidea have significant anti-inflammatory properties, suggesting that the extracts may be useful for relieving pain by reducing inflammation.Antinociceptive activity ofF. deltoidea aqueous extract from leaves was studied by Sulaiman et al. [21] in order to verify using this plant to reduce sensitivity to painful stimuli using chemical and thermal nociception models. Three methods were used in their experiment: acetic acid-induced abdominal writhing, formalin, and a hot plate test. Significant antinociceptive effects ofF. deltoidea leaves were observed in all three methods used when administered 30 minutes prior to pain induction. The results demonstrate thatF. deltoidea leaves do have antinociceptive activity, corroborating its traditional use in treating painful conditions, such as headache and toothache.


## 5.3. Antimelanogenic and Antiphotoaging Effect

Ficus deltoidea was also reported to have antimelanogenic effects in reducing melanogenesis biosynthesis for melanin production [22].Ficus deltoidea extract activity was investigated using cultured B16F1 melanoma cells focusing on the characterization of tyrosinase, the enzyme that is required for melanin production. The extract fromF. deltoidea inhibited mushroom tyrosinase activity and intracellular tyrosinase activity of B16F1. Microphthalmia-associated transcription factor (MITF) expression also decreased whenF. deltoidea extract was applied, suggesting thatF. deltoidea has strong potential for use as a depigmenting agent in cosmetics.Treatment withF. deltoidea extract also significantly inhibited UV-induced TNF-α, IL-1α, IL-6, and COX-2 expression [23]. Decreased collagen synthesis of fibroblasts as a result of UVB exposure returned to normal afterF. deltoidea extract treatment. Furthermore, following UVB irradiation enhanced MMP-1 expression that would usually be observed decreased after treatment with theF. deltoidea extract in a dose-dependent manner. The study concluded that theF. deltoidea extract may have a positive effect against UVB-induced skin damage and that it is useful for antiphotoaging cosmetic products [23].


## 5.4. Antioxidant Effect

The antioxidant property of theF. deltoidea extract was revealed through a total phenolic content and ferric reducing antioxidant potential (FRAP) assay by Omar et al. [3]. It was found that flavan-3-ol monomers and proanthocyanidins contributed 85% of the antioxidant activity of the aqueous extract ofF. deltoidea (Figure 3). In contrast, this figure was 15% for flavones, primarily due to apigenin-6,8-C-diglucoside. Previously, Hakiman and Maziah [24] described another experiment where different aqueous extracts ofF. deltoidea accessions were evaluated for their antioxidant activities using several assays such as FRAP, free radical scavenging assay, total polyphenol, flavonoid, phenolic acid, and vitamin C.Chemical structure of (a) flavan-3-ol monomer and (b) proanthocyanidins.
(a)
(b)


## 5.5. Antiulcerogenic Effect

The potential ofF. deltoidea whole-plant extract as an ulcer healing agent for gastric ulcers caused by ethanol in Sprague Dawley rats was investigated by Zahra et al. [25]. Four groups of rats were used in the experiment: negative controls (treated with distilled water), 250 and 500 mg kg−1 ofF. deltoidea extract, and positive controls (treated with omeprazole). Pretreatment withF. deltoidea extract demonstrated considerably less gastric mucosal lesions produced by ulcerogens compared to the negative control. Predictably, gastric protection was more effective with use of 500 mg kg−1  F. deltoidea extract compared to 250 mg kg−1. The negative control rats showed very severe gastric mucosal damage as well as edema and leucocyte permeation of the submucosal layer, suggesting thatF. deltoidea extract protects against ulcers, demonstrated by the reduction in ulcer areas, inhibition of submucosal edema, and reduced leucocyte infiltration.


## 5.6. Wound Healing Activity

The effects ofF. deltoidea extract for healing of wounds in Sprague Dawley rats were evaluated by Abdulla et al. [26]. The group that was treated with a placebo containing 5% and 10% ofF. deltoidea extracts showed significantly accelerated wound healing in comparison with deionized water (negative control) and blank placebo treatments. The rats treated withF. deltoidea also showed decreased scar width, more fibroblast proliferation, and more collagen fibres accompanied by angiogenesis in the granulation tissue.


## 5.7. Antibacterial Activity

A study by Uyub et al. [27] found thatF. deltoidealeaf extract has antimicrobial activity towardsHelicobacter pylori, the major agent in chronic gastritis and gastric ulcers. Methanol extracts ofF. deltoidea showed highest activity againstH. pylori with an inhibition zone diameter of 12.0
±
0.6 mm followed by petroleum ether 10.0
±
0.6 mm and chloroform extract 8.0
±
0.1 mm. Another study found that ethanol and methanol extracts ofF. deltoidea leaves inhibited growth ofBacillus subtilis[28]. This was further supported by Samah et al. [29] who found methanol extracts of wholeF. deltoidea exhibited antibacterial activity againstStaphylococcus aureus,Bacillus subtilis,Escherichia coli, andPseudomonas aeruginosa and antifungal activity towardsCandida albicans.


## 5.8. Anticancer Effect

Aqueous and ethanol extracts ofF. deltoidea were found to have anticancer activity against human ovarian carcinoma cell line A2780 using a cell-based assay and confirmed by microscopic observation [30]. The plant extracts gave IC50 value of 224.39
±
6.24 μg/mL and 143.03
±
20.21 μg/mL in aqueous and ethanolic extracts, respectively. Both of the plant extracts have different effects on cell growth: the aqueous extract promoted cell detachment, while the ethanolic extract decreased cell proliferation. This might be due to different types of phytochemical property in those two extracts. The study also reported that apoptosis was caused by both extracts at 1000 μg/mL.


## 5.9. Miscellaneous Activity

A study was conducted to assess whether the aqueous and ethanolic extracts ofF. deltoidea leaves possessed antithrombotic activity and to observe the effect of the extracts on sperm quality and testosterone levels in diabetic rats [31]. These rats were administered alloxan monohydrate, which is known to cause an increase in blood glucose level, blood clotting rate, and sperm abnormalities and a decrease in testosterone level. Interestingly, rats given aqueous and ethanolic leaf extracts ofF. deltoidea demonstrated significantly increased testosterone levels, sperm count, and motility and showed a reduction in the blood clotting levels and blood glucose level, as well as a reduction in sperm abnormalities.Samsulrizal et al. [32] conducted another similar study with the same result and they additionally considered lactate dehydrogenase C4 (LDH-C4), a specific isoenzyme in mammalian testes that plays a role in sperm capacitation. The effect ofF. deltoidea on LDH-C4 activity was also seen positive for maintaining healthy sperm. A further study on the effects ofF. deltoidea methanolic extract on the reproductive systems of male rats was conducted by Norrizah et al. [33]. Surprisingly, the study found that the leaf extract decreased testes and epididymis weight, sperm count, and sperm viability. Rats treated with stem extract showed a significant increase in epididymis weight and sperm count and viability, although reduction in testes weight and the number of normal sperm morphology was observed.


## 6. Cytotoxicity Activity

Comprehensive attempts have been made on efficiency and safety studies onF. deltoidea [34–36]. A study by Shafaei et al. [35] confirmed thatF. deltoidea leaves do not contain toxic elements by evaluating toxicological elements (lead, cadmium, arsenic, and mercury) using atomic absorption spectroscopy technique, standardisation parameters (moisture, volatile, total ash, and acid insoluble ash), and Microbial Limit Test (MLT) for microbial contamination.A genotoxicity study was performed using the Ames test with the TA98 and TA100Salmonella typhimurium strains and showed theF. deltoidea extract did not have any potential to induce mutations in the presence or absence of S9 metabolic activation [36]. Further study on the acute toxicity showed that the LD50 of the extract was greater than 5000 mg/kg on Sprague Dawley rats treated with five different doses of the extract. In the subchronic study, there were no significant adverse effects on body weight, organ weight, food consumption, mortality, haematology, histopathology, and clinical chemistry, except an increase in the serum urea level [36].


## 7. Conclusions

The scientific research onF. deltoidea indicated that this plant has received increasing interest in recent years.Ficus deltoideahas been reported to have beneficial pharmaceutical uses as an antidiabetic, anti-inflammatory, antinociceptive, antimelanogenic, antiphotoaging, antioxidant, antiulcerogenic, and antibacterial agent. Phytochemical and pharmaceutical studies have validated the traditional uses ofF. deltoidea; however, there is a need to investigate the chemical or bioactive components that are responsible for providing the specific properties. The principle bioactive metabolites have to be examined for their bioactivities and their mechanism of action needs to be determined, in conjunction with analysing the pharmacokinetics and physiological pathways of specific compounds inF. deltoidea. These may help to strengthen our understanding of this highly therapeutic plant for commercial exploitation.



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*Source: 902734-2014-03-18.xml*