# Cervical Cancer Screening Guidelines in the Postvaccination Era: Review of the Literature

Authors: Carlo A. Liverani; Jacopo Di Giuseppe; Luca Giannella; Giovanni Delli Carpini; Andrea Ciavattini
Journal: Journal of Oncology
 (2020)

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## Abstract

Cervical cancer is relatively rare in high-income countries, where organized screening programs are in place, as well as opportunistic ones. As the human papillomavirus (HPV) vaccination rates increase, the prevalence of cervical precancers and cancers is going to decrease rapidly very soon, even if, in the most optimistic scenario, it is unlikely that optimal vaccination coverage will be achieved. Then, the optimal screening paradigm for cervical cancer prevention in the postvaccination era is still debated. Screening guidelines are being developed with the aim of reducing the number of tests a woman needs during her lifetime, in order to receive the maximum benefit from screening, while decreasing potential harms that may result with the use of a screening strategy (overdiagnosis, overtreatment, anxiety, and costs). With this purpose in mind, new management guidelines for cervical cancer screening abnormalities are recommendations based on risks, not on results. This review aims to summarize the process that led to the introduction of the HPV DNA test in screening programs and the different screening strategies. Moreover, it aims to introduce the new risk-based guidelines for the future, where full HPV genotyping can resize the risk on the basis of specific high-risk genotypes. In the same way, the data regarding HPV vaccination could be introduced as soon as women vaccinated with the nonavalent vaccine reach the screening age, with the recommendation of a prolonged screening interval.


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## Body

## 1. Introduction

Cervical cancer is the seventh most common cancer in European women, accounting for about 3.8% of the total [1]. It is relatively rare in high-income countries, where organized screening programs are in place, as well as opportunistic ones.Multiple studies have provided mounting evidence supporting human papillomavirus (HPV) DNA-based screening provides greater protection against invasive cervical carcinomas compared with cytological screening [1–4]. Nonetheless, HPV testing has created confusion for many specialists as well as for most of the patients, who often misinterpret the implication of a positive result. When a new test is introduced into daily clinical practice, a misuse is always expected. If the new test is introduced in a screening guideline, then it will be employed even more frequently out of recommendations, because it is presumed to be very effective.Since HPV DNA testing has been introduced for cervical cancer screening in women aged 30 to 65 years both in the American and in the European guidelines [5–10] and the costs of the new assays have declined and become affordable, HPV tests are being more frequently used in situations where they are not needed. It is well known that the natural history of HPV infections implies frequent clearance of the virus in immunocompetent patients, as well as high rates of spontaneous regression of low-grade lesions [11–13]. However, not rarely clinicians start treating HPV infections detected by molecular methods with surgery, laser, cryotherapy, interferon, and 5-florouracil. Then, multiple preventive, diagnostic, and therapeutic activities are initiated, both in women and in their partners, with strict follow-up programs, more tests, and more interventions. What many health professionals actually do is test women under 25 years of age: rescreen every 1-2 years, test for low-risk HPV types, test anal, vulvar, penile, and oral sites, and test male partners [14].All these indications are not recommended and may lead to wrong decisions, with well-documented but poorly recognized ill effects [15, 16]. Overtreatment refers not only to the well-studied unfavorable obstetric outcomes following cervical conization procedures [17], but also to possible cervical stenosis or even hematometra requiring surgery, damage to the uterine cervix compromising regular follow-up, and a heavy psychological burden to women and their sexual partners. Intricate algorithms are not easy to follow and poor adherence to guidelines recommendations affect the prerequisite upon which HPV testing strategies are based [18, 19]. Moreover, while HPV primary testing could assume a central role within an organized vaccination and screening program, the opportunistic screening environment in some countries does not yield itself to primary HPV screening and may result in underscreening or lack of screening [20].Similarly, invasive cervical cancer disproportionately affects women without sufficient access to care, with higher rates among minority groups in higher-income countries and women in low-resource regions of the world [21]. Since equity in access is one of the main objectives of organized screening programs, the introduction of HPV test may suffer from the effect of social inequality: some authors speculated noncompliance to protocols and interaction with opportunistic screening can increase the existing disparities because HPV will be much more expensive than the Pap test. Moreover, longer intervals may disrupt women’s habits and thus have detrimental effects on coverage [22].The same disparity also exists for HPV vaccination: only in industrialized countries, a decline of cervical precancers and cancers is expected, with the increase of the vaccination rate. However, even in the most optimistic scenario, it is unlikely that optimal vaccination coverage will be achieved [23, 24]. Then, what could be the optimal screening paradigm for cervical cancer prevention in the postvaccination era?This review aims to summarize the process that led to the introduction of the HPV DNA test in screening programs and the different screening strategies in some of the main areas of the world. Moreover, it aims to introduce the new risk-based guidelines for the future, in the postvaccination era.This review was based on literature search in PubMed (published before October 2020), concerning national and international screening guidelines for cervical cancer.


## 2. Materials and Methods

### 2.1. Accuracy of the Screening Test

All women in the world, regardless of their sexual orientation, must be considered candidates for cervical cancer screening. However, in any population, with any screening program and with any test employed, there will always be some cases of cervical neoplastic and preneoplastic lesions that will not be detected; no test is perfect and the advantages and disadvantages must be carefully weighed in each local reality, when deciding which test or which tests should be adopted.The sensitivity of conventional cytology, often reported around 50%, was higher than 80% in some studies [25, 26]. In a Japanese study, considering high-grade squamous lesions, the sensitivity of the Pap test in detecting cervical cancer was 94.7% [27]. Overall, the sensitivity of the Pap test varies from 50% to 80%, while the specificity from 70% to 90%.A large number of studies evaluated molecular biology tests (HPV tests) in primary screening [28, 29]. The sensitivity of the HPV test had often been assessed for cervical intraepithelial neoplasia (CIN) lesions or worse (CIN 2+) [30], but when it was assessed for CIN 3 lesions or worse (CIN 3+), the sensitivity of the HPV test was comparable to that of conventional cytology. In any case, the higher detection of CIN did not lead to an absolute reduction in the incidence of invasive cancers, given the high probability of nonprogression [31]. Both the specificity and the positive predictive values were lower for the HPV test compared to the Pap smear. The combination of the Pap test and HPV test showed greater sensitivity than cytology, but less specificity [32]. In two Italian trials [33, 34], although a CIN 2+ lesion was detected more often with the combination of the Pap test and HPV test compared to cytology alone, the positive predictive value was lower with the combination of the two methods. Regarding HPV testing with cytological triage, a Swedish study showed that the increased incidence of CIN 2 lesions diagnosed at the initial screening was not followed by a statistically significant reduction in CIN 2 lesions to a subsequent screening. Therefore, although the combination of the HPV test and cytology seems to increase the sensitivity, it is known that many lesions can often regress spontaneously [35]. Expanding the detection of CIN could increase overdiagnosis, since the vast majority of low-grade lesions regress in 10 years or less (CIN 1 and 2 regress in 87.7% and 82.9% of the cases, respectively; CIN 1 and 2 progress to a more severe lesion in 9.9% and 32.0% of the cases, respectively) [13]. In a randomised controlled trial (the ARTISTIC study), when combining the first and the second round of screening, the proportion of women with CIN 3+ lesions was similar in the Pap test group and in the Pap test/HPV test group; the sensitivity of thin-layer cytology was slightly higher than that of the HPV test with cytological triage or the Pap test with the HPV triage test also in detecting CIN 3+ lesions [36].Eventually, sensitivity should not be overstated [37]. As reported above, less than a third of high-grade lesions progress to cancer [13, 38, 39]. Unfortunately in the United States, one doctor out of four is testing for both high-risk and low-risk HPV types; about 60% of health care providers choose cotesting (the Pap test and HPV test) under the age of 30 years; there is a widespread use of molecular tests for high-grade lesions already histologically confirmed; the cotest is repeated annually or every two years. All these habits are wrong and may lead to waste of resources and possible harms caused by overtreatment [40–42]. Moreover, according to the data reported by the World Health Organization (WHO), there are several HPV testing products available (29 tests) [43]. Some of these are able to detect a group of high or low-risk genotypes. Only a small part is able to type the viral genotype, in particular for HPV 16 and 18. All these make interpretation of the result even more difficult. In Italy, there are even 12 different types of tests validated and usable for screening, but which provide different results [44]. Another concern raised is that some studies have demonstrated that up to 10% of cervical tissues in US tumor registries do not contain detectible HPV [45–48] and that 37% of cervical adenocarcinomas around the world are HPV negative [49].Nonetheless, the publication of Ronco et al. in 2014, analyzing the follow-up for the four main randomised controlled trials on the effectiveness of screening based on the HPV test, definitely showed that the cumulative probability of developing cervical cancer in women included in the Pap test program was greater than those included in the Pap test/HPV test program, leading to the conclusion that HPV-based strategies confer 60–70% greater protection than cervical cytology [2]. This study was not free from criticism [50], but influenced many associations to change their current guidelines.


## 2.1. Accuracy of the Screening Test

All women in the world, regardless of their sexual orientation, must be considered candidates for cervical cancer screening. However, in any population, with any screening program and with any test employed, there will always be some cases of cervical neoplastic and preneoplastic lesions that will not be detected; no test is perfect and the advantages and disadvantages must be carefully weighed in each local reality, when deciding which test or which tests should be adopted.The sensitivity of conventional cytology, often reported around 50%, was higher than 80% in some studies [25, 26]. In a Japanese study, considering high-grade squamous lesions, the sensitivity of the Pap test in detecting cervical cancer was 94.7% [27]. Overall, the sensitivity of the Pap test varies from 50% to 80%, while the specificity from 70% to 90%.A large number of studies evaluated molecular biology tests (HPV tests) in primary screening [28, 29]. The sensitivity of the HPV test had often been assessed for cervical intraepithelial neoplasia (CIN) lesions or worse (CIN 2+) [30], but when it was assessed for CIN 3 lesions or worse (CIN 3+), the sensitivity of the HPV test was comparable to that of conventional cytology. In any case, the higher detection of CIN did not lead to an absolute reduction in the incidence of invasive cancers, given the high probability of nonprogression [31]. Both the specificity and the positive predictive values were lower for the HPV test compared to the Pap smear. The combination of the Pap test and HPV test showed greater sensitivity than cytology, but less specificity [32]. In two Italian trials [33, 34], although a CIN 2+ lesion was detected more often with the combination of the Pap test and HPV test compared to cytology alone, the positive predictive value was lower with the combination of the two methods. Regarding HPV testing with cytological triage, a Swedish study showed that the increased incidence of CIN 2 lesions diagnosed at the initial screening was not followed by a statistically significant reduction in CIN 2 lesions to a subsequent screening. Therefore, although the combination of the HPV test and cytology seems to increase the sensitivity, it is known that many lesions can often regress spontaneously [35]. Expanding the detection of CIN could increase overdiagnosis, since the vast majority of low-grade lesions regress in 10 years or less (CIN 1 and 2 regress in 87.7% and 82.9% of the cases, respectively; CIN 1 and 2 progress to a more severe lesion in 9.9% and 32.0% of the cases, respectively) [13]. In a randomised controlled trial (the ARTISTIC study), when combining the first and the second round of screening, the proportion of women with CIN 3+ lesions was similar in the Pap test group and in the Pap test/HPV test group; the sensitivity of thin-layer cytology was slightly higher than that of the HPV test with cytological triage or the Pap test with the HPV triage test also in detecting CIN 3+ lesions [36].Eventually, sensitivity should not be overstated [37]. As reported above, less than a third of high-grade lesions progress to cancer [13, 38, 39]. Unfortunately in the United States, one doctor out of four is testing for both high-risk and low-risk HPV types; about 60% of health care providers choose cotesting (the Pap test and HPV test) under the age of 30 years; there is a widespread use of molecular tests for high-grade lesions already histologically confirmed; the cotest is repeated annually or every two years. All these habits are wrong and may lead to waste of resources and possible harms caused by overtreatment [40–42]. Moreover, according to the data reported by the World Health Organization (WHO), there are several HPV testing products available (29 tests) [43]. Some of these are able to detect a group of high or low-risk genotypes. Only a small part is able to type the viral genotype, in particular for HPV 16 and 18. All these make interpretation of the result even more difficult. In Italy, there are even 12 different types of tests validated and usable for screening, but which provide different results [44]. Another concern raised is that some studies have demonstrated that up to 10% of cervical tissues in US tumor registries do not contain detectible HPV [45–48] and that 37% of cervical adenocarcinomas around the world are HPV negative [49].Nonetheless, the publication of Ronco et al. in 2014, analyzing the follow-up for the four main randomised controlled trials on the effectiveness of screening based on the HPV test, definitely showed that the cumulative probability of developing cervical cancer in women included in the Pap test program was greater than those included in the Pap test/HPV test program, leading to the conclusion that HPV-based strategies confer 60–70% greater protection than cervical cytology [2]. This study was not free from criticism [50], but influenced many associations to change their current guidelines.


## 3. Cervical Cancer Screening around the World

Table1 shows the worldwide HPV vaccine coverage among female population aged 10–20 [51], the cervical cancer incidence (by continent) [52], and the health care systems (by countries).Table 1
Worldwide human papilloma virus coverages among female population aged 10–20 years, the cervical cancer incidence (by continent), and the health care systems (by countries).
Geographical regionHPV vaccine coverageCervical cancer incidence (cumulative risk 0–74) (%)Africa1.2% (0.6–2.0)Eastern Africa4.34Middle Africa3.02Northern Africa0.82Southern Africa4.20Western Africa3.47Asia1.2% (0.7–1.8)Eastern Asia1.09South-Eastern Asia1.86South-Central Asia1.41Western Asia0.45Europe36.4% (30.3–42.9)Central and Eastern Europe1.58Western Europe0.66Southern Europe0.77Northern Europe0.85Latin America and Caribbean22.1% (12.7–32.6)Caribbean1.54Central America1.32South America1.56North America53.4% (27.1–85.6)North America0.62Oceania41.1% (21.5–64.0)Australia and New Zealand0.55Melanesia2.60Polynesia1.16Micronesia2.03Data in parentheses are 95% confidence interval.(i)
Nonuniversal insurance system: Bangladesh, Egypt, Ethiopia, Jordan, Kenya, Nigeria, Paraguay, Tanzania, Uganda, the United States, and Yemen(ii)
Universal government-funded: Australia, Bahrain, Bhutan, Botswana, Brazil, Brunei Canada, Cuba, Denmark, Finland, Georgia, Greece, Iceland, India, Ireland, Italy, Kuwait, Malaysia, Malta, New Zealand, North Korea, Norway, Oman, Portugal, San Marino, Saudi Arabia, South Africa, Spain, Sri Lanka, Sweden, Taiwan, Trinidad and Tobago, and the United Kingdom(iii)
Universal private health insurance system: Israel, Liechtenstein, Netherlands, and Switzerland(iv)
Universal public insurance system: Albania, Andorra, Belgium, Bulgaria, China and Hong Kong, Colombia, Croatia, Czech Republic, Estonia, France, Hungary, Iran, Japan, Latvia, Lithuania, Luxembourg, Monaco, Moldova, Montenegro, Poland, Qatar, Romania, Russia Serbia, Singapore, Slovakia, Slovenia, South Korea, Ukraine, and the United Arab Emirates(v)
Universal public-private insurance system: Algeria, Argentina, Austria, Chile, Cyprus, Germany, Mexico, Peru, and Turkey


### 3.1. Europe

In Europe, about 60,000 new cases of cervical cancer are being diagnosed every year, with 25,000 deaths per year [53]. Despite the implementation of vaccination programs, screening for cervical cancer precursors and treatment of detected lesions will remain the most effective tool for cervical cancer prevention in the medium-long term. Cervical cytology has been the cornerstone of European screening programs: according to data from the International Agency for Research on Cancer, Pap smears performed every 3–5 years reduce the incidence of cervical cancer by 80% [54]. Screening is “organized” in the Nordic countries, in the United Kingdom, in Holland, in Poland, Croatia and Slovenia, Belgium, and the Czech Republic, and in many parts of Italy [54, 55]; screening is “opportunistic” in the remaining countries (with a low cost-effectiveness ratio: overscreened women and underscreened women). Table 2 shows the programmes of cervical cancer screening and human papillomavirus (HPV) vaccination in European countries [55].Table 2
Programmes of cervical cancer screening and HPV vaccination in European countries.
CountriesCervical screening program (starting year)Screening ageScreening intervalPrimary test usedHPV vaccine (national vaccination program) (year of initiation)AustriaOpportunistic18+ or 2 years after sexual onset1CC2014BelgiumOrganized population-based, in some regions, rollout ongoing, 201325–643CC & LBC2010BulgariaOpportunisticNANANA-CroatiaOrganized population-based, rollout ongoing, 201225–643CC2016CyprusOpportunisticNANAN/A2016Czech republicOrganized population-based, rollout ongoing, 200815+1CC2012DenmarkOrganized population-based, 200623–643 (ages: 23–59); 5 (ages: 60–64)LBC (ages: 23–59); HPV primary test (ages:60–64)2009EstoniaOrganized population-based, 200630–595CC2018FinlandOrganized population-based (depending on the region), 196325–655CC, HPV primary testing in some regions2013FranceTransitioning to organized population-based planned, 199125–643 (CC), 5 (HPV test)CC & LBC (ages: 25–64); HPV primary testing in regional pilot projects (ages: 30–64)2007GermanyTransitioning to organized population-based planned, 197120+1CC, HPV primary testing in implementation2007GreeceOpportunisticSexual onset1CC2008HungaryOrganized population-based, rollout ongoing, 200325–653CC2014IrelandOrganized population-based, rollout ongoing, 200825–603 (ages: 25–44); 5 (ages: 45–60)LBC2010ItalyOrganized population-based, rollout ongoing, depending on the region, 198925–643 (ages: 25–30/35); 5 (ages: 30/35–64)CC & LBC (ages: 25–30/35); HPV primary testing in some regions (ages: 30/34–64)2008LatviaOrganized population-based, 200925–693CC2010LithuaniaOrganized population-based, rollout ongoing, 200425–593CC2016LuxembourgOpportunistic15+1LBC2008MaltaOrganized population-based, piloting, 201525–353CC2012NetherlandsOrganized population-based, 197030–645HPV primary testing2010NorwayOrganized population-based, 200625–693CC & LBC, regional pilot for HPV primary testing,2009PolandOrganized population-based, 200625–593CC (ages: 25–59); regional pilot for cotesting (ages: 30–59)—PortugalOrganized population-based, in some regions, rollout ongoing, depending on the region, 199025–593Cotesting in some regions2008RomaniaOrganized population-based, in some regions, rollout ongoing, 201225–645Cotesting in some regions—SlovakiaTransitioning to organized population-based, 200823–64Yearly x 2; then 3 yearlyCC—SloveniaOrganized population-based, (2003)20–64Yearly x 2; then 3 yearlyCC2009SpainOpportunistic (depending on the region)25–653CC2007SwedenOrganized population-based, 196723–603 (ages: 23–50); 5 (ages: 51–60)HPV test replacing CC & LBC2012SwitzerlandOpportunistic21/702 (ages: sexual onset/21–29); 3 (ages: 30–70)CC & LBC2008The United KingdomOrganized population-based, 198825–643 (ages: 25–49); 5 (ages: 50–64)HPV primary testing in implementation2008CC = conventional cytology; LBC = liquid-based cytology; N/A: not available.The Council of the European Union (which represents the 27 member states) has always recommended the cervical cancer screening intervals reported in Table3 [56].Table 3
Cervical cancer screening intervals with the Pap test according to the European Union recommendation (depending on the available resources in each member state).
AgeMethod and intervalsOnset of screening: between 20-25 and 30 yearsPap test every 3–5 yearsEnd of screening: 60–65 yearsA supplement to the second edition of the European guidelines [6] was published in September 2015 introducing the HPV DNA test alone, as the primary screening test in European countries (as opposed to the American guidelines, which recommended cotesting), together with the implementation of HPV vaccination programs. Notably, it was reported that(1)
the primary test for the detection of high-risk oncogenic HPV types can be used within an organized screening program,(2)
HPV test is not recommended outside of organized programs. Cotesting (the Pap test and HPV test) should be avoided: only one primary test, Pap test or HPV test, should be employed,(3)
routine screening with HPV tests should be used starting at the age of 35 and in any case not below 30 years (in the age group between 30 and 34 years, the available evidence was not considered sufficient to recommend HPV DNA testing). Under 30–34 years of age, the recommended screening test is the Pap test,(4)
the screening interval in women with the HPV negative test must be at least 5 years, with the possibility of extension up to 10 years, depending on the age and history of the patient. Screening can cease at the age of 60–65, provided that the woman has a recent negative test, and(5)
the management of a woman with a positive HPV test involves triage with cervical cytology, while direct referral to colposcopy is not recommended.Table4 reports cervical cancer screening intervals with the HPV test and Pap test, according to European Union recommendations.Table 4
Cervical cancer screening intervals with the HPV test and pap test, according to the European union recommendation (depending on the available resources in each member state).
AgeMethod and intervalsScreening starts between 20-25 and 30 yearsPap test every 3–5 yearsAn alternative starting at 30–35 yearsHPV DNA test every 5–10 yearsEnd of screening: 60–65 yearsFurthermore, the European Council recommends high quality standards in all phases of the program (invitation, screening, diagnosis, treatment of detected lesions, and post-treatment follow-up), and urges to discourage opportunistic screening as well as to refrain from introducing new primary screening programs in the absence of population studies [6, 56].Despite the consistent evidence that organized programs are more efficient than opportunistic screening, and despite the recommendations of the European Council, the health authorities of many European countries have not yet implemented a nationally organized cervical cancer screening program [57, 58]. The Italian health system, by means of all the regions in the country, is committed to changing the screening program by the end of 2020. However, a great variability still exists from region to region [59], with local realities employing cervical cytology every 3 years from 25 to 64 years of age, and regions employing cytology from 25 to 29 years and then shifting to HPV testing every 5 years from 30 to 64, and situations in which screening is spontaneous and unorganized, with differences even within a same region. On the other hand, in Holland, the HPV DNA test program was established at a national level, at 5-year intervals from 30 to 40 years, and at 10-year intervals from 40 to 60 years. No screening is recommended for women under 30 and over 64 years [60].In the United Kingdom, cervical screening follows the European Guidelines, but liquid-based cytology is recommended instead of conventional cytology (Table5). HPV screening is being piloted across England to assess how this approach can be used across the programme as a whole [61, 62].Table 5
Cervical cancer screening intervals with the Pap test, according to the United Kingdom recommendation.
AgeMethod and intervals<25 yearsNo screening25–49 yearsLiquid-based cytology every 3 years50–64 yearsLiquid-based cytology every 5 years≥65 yearsScreening for women who have had recent abnormal tests or who have not had an adequate screening test reported since age 50


### 3.2. The Russian Federation

Cervical cancer is the second cause of death in women in Russia. About 70% of all cancers are still diagnosed in advanced stages. No organized screening program is in place, but the Minister of Health recommends screening as for the WHO and European standards [63].


### 3.3. The United States of America

The American guidelines are the most comprehensive, undoubtedly validated by sufficient scientific evidence in their effort to achieve the maximum cost-effectiveness ratio, but certainly complex in practical management. Recommendations have a “grading” with 5 degrees (“A” when the benefit is high, “B” when it is moderate, “C” when it is low, “D” when the disadvantages outweigh the advantages, and “I” when the scientific evidence is not yet sufficient to determine the balance between benefits and harms), and quality of evidence at 3 levels (“high,” “moderate,” and “low”). The recommendations were shared, until July 2020, by the American Cancer Society (ACS), the American Society for Colposcopy and Cervical Pathology (ASCCP), and the American Society for Clinical Pathology (ASCP) (Table6) [7, 8]. In July 2020, the ACS produced a guideline update that leads to substantial changes [10]. Until July 2020, the ACS was in accordance with other American societies.Table 6
Cervical cancer screening intervals with the HPV test and Pap test, according to the American Society for Colposcopy and Cervical Pathology (ASCCP) and the American Society for Clinical Pathology (ASCP).
AgeMethods<21 years (Grade D)No screening (in the case of a Pap smear ASC-US: HPV testing is not recommended)21–29 years (Grade A)Pap smear every 3 years30–65 years for women who want to extend their screening interval (Grade A)Cotest (the Pap test + HPV DNA test) every 5 years30–65 years in women who choose to be tested more often (Grade A)Pap smear every 3 years>65 years (Grade D)No screening in women with previous adequate screening (i.e., at least two negative results in the last 10 years, with at least one in the last 5) in women with a history of CIN 2 or worse lesions: Continue routine screening for at least 20 yearsScreening posthysterectomy (Grade D)No screening (women with removal of the cervix and no history of a high-grade precancerous lesion in the last 20 years, or cervical cancer ever)After HPV vaccination (Grade C)Age-based recommendations for the general population (at the moment according to the same guidelines as women who have not been vaccinated)The most recent ACS guideline (July 2020) [10] calls for substantial changes to the screening method: the ACS recommends that individuals with a cervix initiate cervical cancer screening at age 25 y and undergo primary HPV testing every 5 y through age 65 y (preferred). If primary HPV testing is not available, individuals aged 25–65 y should be screened with cotesting (HPV testing in combination with cytology) every 5 y or cytology alone every 3 y (acceptable) (strong recommendation). A cotesting or cytology testing alone are included as acceptable options for cervical cancer screening because access to primary HPV testing with a test approved by the FDA for primary screening may be limited in some settings. The guideline is transitional; that is, options for screening with cotesting or cytology alone are provided but should be phased out once full access to primary HPV testing for cervical cancer screening is available without barriers [10].The U.S. Preventive Services Task Force (USPSTF), probably the most rigorous in the formulation of the guidelines, prepared a separate document in which women aged 30 to 65 years should be screened every 3 years with cervical cytology alone, or every 5 years with high-risk HPV testing alone or in combination with cytology [11].The cotest (the Pap test + HPV test) is only offered in women aged 30 to 65 years, provided that the 5-year rescreening interval is respected. This approach is defined “preferable” for the ASCCP/ASCP, while it is defined “acceptable” for the ACS/USPSTF. HPV testing as a standalone test (i.e., without cytology) was not recommended.A study [64] was conducted to verify the ideal strategy for cervical cancer screening, which should offer the maximum sensitivity to minimize the risk of missing the disease, together with the maximum specificity to reduce the rate of false-positive cases. This analysis involved 34,254 women aged ≥30 years, extrapolated from the ATHENA (Addressing the Need for Advanced HPV Diagnostics) trial, with an average age of 44.7 years. 8.4% of women were HPV positive, and 5.7% had an abnormal Pap smear. Ten different screening strategies were compared. The most attractive for the “benefits” versus “harms” analysis was the four strategies that involved the use of the HPV test with genotyping for HPV 16/18 (two of which in the cotest with cytology). The two strategies that would seem to optimize the balance between sensitivity and specificity are the cotest with genotyping and triage with Pap LSIL (strategy no. 5) and the HPV test with genotyping and triage with Pap ASC-US (strategy no. 9). The latter led to a 50% reduction in the number of screening tests required, as well as being slightly more sensitive and requiring slightly less colposcopies to detect a CIN 3 or worse lesion. In conclusion [64], according to the authors, to employ in primary screening, a strategy involving the use of the HPV test with triage of HPV-positive women through a combination of genotyping for HPV 16/18 and cytology would provide a good balance between obtaining of maximum sensitivity and specificity, limiting the number of colposcopy referrals. However, it must be noted that the study in question is a post hoc analysis (the ATHENA study had another objective); moreover, it was impossible to know how many high-grade lesions or worse would have been diagnosed at the next screening or during the follow-up.Last but not least, compliance with recommendations to follow-up is essential; otherwise, the assumptions of the entire algorithm collapse. These strategies are therefore a little too intricated, difficult to apply in real practice as far as regards doctors’ attitudes as well as patients’ preferences. The American algorithms are also very complex for professionals and, in their attempt to maintain a correct cost-effectiveness ratio, they risk being difficult to use and above all not being respected [7–9, 18, 19, 65]. For instance, the most common rescreening interval after a negative HPV result was found to be only 12 months in the United States [60]. Interim Clinical Guidance for use of primary high-risk human papillomavirus testing for cervical cancer screening has also been published introducing in the USA the use of the HPV test as the only primary screening method [3, 66]. However, these works are burdened by heavy conflicts of interest (Merck, GSK, Hologic, Roche, Gen Probe, Becton-Dickinson, Cepheid, and Genentech). In Wright’s article, even one of the sponsors (Roche Molecular Systems, Pleasanton, CA) was involved in all aspects of the design and conduct of the study: collection, management, analysis, and interpretation of the data; two employees of the pharmaceutical company were integral to the preparation of the manuscript, and the sponsor reviewed the final version of the manuscript [3].In a conflict-free article [67], however, the Cobas test was evaluated on more than 33,000 Pap smears, detecting a 59.7% negative test rate in cases with abnormal cytology and a 38.2% negative test rate in a subset of histologically confirmed CIN 2–3 lesions. Obviously, the authors’ conclusion is that the HPV Cobas test cannot be used in primary screening, as it would provide a false guarantee to doctors and patients, delaying appropriate treatment.The latest recommendations from the Atlanta Centers for Disease Control and Prevention (CDC) (June 2015) clearly stated that the HPV test should not be recommended for screening for cervical cancer as a standalone test (i.e., without a concurrent Pap test) [68].Finally, in 2020, the ASCCP has introduced new management guidelines for cervical cancer screening abnormalities, revolutionizing the future of risk-driven recommendations for this topic (see below) [69].


### 3.4. Canada

In 2011, around 1.300 new cases of cervical cancer were recorded in Canada (with about 350 deaths). There has been no reduction in cervical cancer mortality in Canada since 1970 in women aged 20–24 years (i.e., since the start of screening) [70].The recommendations follow a simpler “grading” than the USA, with only 2 degrees: “strong” or “weak”; on the other side, the quality of evidence is divided into 4 levels: “high,” “moderate,” “low,” and “very low.” Three-year intervals are the right balance between the small potential to increase the benefits obtainable with shorter intervals and the greater potential for harms resulting from the increase in tests and procedures related to more frequent screening. Screening by the HPV test, alone or in combination with the Pap test, is not recommended (as there was insufficient evidence on its effect on mortality and incidence of invasive cancer). Canadian guidelines also state that organized screening is more effective than opportunistic screening. The sensitivity and specificity of thin-layer and conventional cytology are similar. An increase or decrease in screening may be appropriate for women with different risk profiles. The recommendations are reported in Table7.Table 7
Cervical cancer screening intervals according to the Canadian task force on preventive health care recommendations on screening for cervical cancer.
AgeMethodGrade of recommendation/quality of evidence<20 yearsNo screeningStrong/high20–24 yearsNo screeningWeak/moderate25–29 yearsPap smear every 3 yearsWeak/moderate30–69 yearsPap smear every 3 yearsStrong/high>70 a yearsNo screening if at least three consecutive negative Pap tests in the past 10 years; otherwise, continue screening until three consecutive negative resultsWeak/lowThese recommendations do not apply to women with cervical cancer symptoms or previous abnormal screening test results; women whose uterine cervix has been completely removed; immunosuppressed women for HIV, organ transplants, chemotherapy, or chronic use of corticosteroids; and women with reduced life expectancy so that they cannot benefit from screening. Finally, it is recommended that clinicians be aware of the values, preferences, and beliefs of each woman. HPV DNA testing as primary screening strategy is being introduced in many provinces.


### 3.5. Latin America

Screening programs for cervical cancer in Latin America and the Caribbean had not been successful in many situations, and incidence and mortality are still high. Even after the introduction of screening programs, incidence and mortality for cervical cancer did not change in many countries, because the coverage of screening is inadequate in rural areas [71, 72]. Of note, Chile, Colombia, Mexico, Peru, and more recently Argentina, are actively evaluating and proposing new strategies for cervical cancer control [73]. In consequence, countries such as Chile, Colombia, and Costa Rica are registering reductions in mortality rates for cervical cancer [72].


### 3.6. Australia

Until 2017, Australian recommendations were very simple and effective. The first Pap smear was proposed between 18 and 20 years of age, or 1–2 years after the onset of sexual activity (depending on which of the two events occurs later) and then repeated every 2 years until 69 years.The “National Cervical Screening Program” was revised in 2017 [74]. Therefore, starting from this date, HPV testing was introduced for primary screening (Table 8).Table 8
Cervical cancer screening intervals according to the Australian guidelines.
AgeMethod and intervals25–74 yearsHPV DNA every 5 years>74 yearsNo screening (at the discretion of the caregiver, if the previous results are normal)The new cervical cancer screening pathway is a risk-based approach in which partial genotyping is used to classify the type of HPV into one of two groups: oncogenic HPV 16/18 or oncogenic HPV types not 16/18, as a pooled result. The Australian risk-based approach, according to HPV test result, is reported in Table9.Table 9
Cervical screening test results for clinician-collected sample according to the Australian guidelines.
Risk of significant cervical abnormalitiesHPV test resultReflex liquid-based cytology resultRecommended managementLow-risk resultHPV not detected—Return to screening in 5 yearsIntermediate-risk resultHPV not 16/18 detectedNegative, possible LSIL or LSILRepeat HPV test in 12 monthsHigher-risk resultHPV not 16/18 detectedPossible HSIL or HSILRefer to specialist (colposcopy)HPV 16/18 detectedAny LBC resultRefer to specialist (colposcopy)—Unsatisfactory HPV test—Collect new sample for the HPV test in 6–12 weeks—HPV not 16/18 detectedUnsatisfactoryCollect new sample for LBC only in 6–12 weeksCotesting is recommended for symptomatic patients (usually abnormal vaginal bleeding) requiring investigation, patients exposed to diethylstilbestrol (DES), patients undergoing Test of Cure (TOC) surveillance, and patients who have been treated for glandular abnormalities.


### 3.7. Japan

Cervical cancer is the ninth leading cause of cancer death in women in Japan. In 2018, about 13.277 new cervical cancer cases were diagnosed in Japan. Recommendations have a 5-degree “grading” as in the USA (“A” and “B” grades indicate when recommendations are valid for both mass and opportunistic screening programs, “C” when they are not valid for mass screening, “D” when they are neither valid for mass nor opportunistic screening, and “I” refers to screening methods in which there is insufficient evidence), and quality of evidence up to 8 levels (based on the type of study: design, quality, and consistency of the study) [75]. Screening guidelines were formulated on the basis of the balance between benefits and harms recommendations for population-based and opportunistic. Five cervical cancer screening methods were evaluated (three of them with the HPV test), after considering 3.450 articles published from January 1985 to October 2007. After a systematic review of the literature, 161 articles were selected and 66 were confirmed. The results of 33 studies were considered satisfactory and the scientific evidence sufficient to evaluate the effect of screening by conventional cytology. The accuracy of thin-layer cytology was equivalent to that of conventional cytology. Although HPV tests and combination methods have shown high sensitivity, no studies have evaluated the reduction in mortality from cervical cancer. In mass screening, as well as in opportunistic screening, conventional or thin-layer cytology is recommended, given sufficient scientific evidence (Grade B). Cervical cancer screening using the HPV test alone or in combination with cervical cytology was not recommended in mass screening, given insufficient scientific evidence (Grade I). Since 2003, screening in Japan has been carried out, as reported in Table 10.Table 10
Cervical cancer screening intervals according to the Japanese guidelines.
AgeMethod and intervals>20 yearsPap test every 2 yearsGiven the different health system in Japan compared to other countries, it was not considered appropriate to modify the current recommendations. To reduce cervical cancer mortality in Japan, it is rather necessary to improve adherence to screening programs and the appropriateness of managing the lesions detected [75]. At the same time, a retrospective study on 3.804 women aged ≥20 years followed for 5 years (from 2005 to 2010) [76], assessed how the incidence rates of cytological abnormalities, CIN, and cervical cancer vary according to age. Under the age of 40, about 5% of women had an abnormal Pap test result, about 3% had CIN, and 0.5% developed a CIN within two years. In women aged between 40 and 49, less than 4% had a cytological abnormality within two years and approximately 5% within three years. In the group between 50 and 59 years old, less than 2% had a cytological abnormality within two years and about 3% within three years. While 0.1% of women developed a CIN in the 40–49 year group within three years, none in the 50–59 year group developed a CIN within three years. In women aged 60 and over, less than 3% had an abnormal Pap test result, less than 0.5% developed a CIN within 5 years, and none developed cancer in the same period. According to the results of this study, the author proposed the following modification [76]: Pap test every 2 years between 20 and 40 years, every 2 or 3 years between 40 and 59 years, and every 5 years after 60 years.


### 3.8. China

Although in China today there are several excellent programs for cervical cancer screening in large cities, these programs cover only a minimal part of the whole population in the country. To achieve a significant reduction of the incidence of invasive cervical cancer with concomitant reduction in the death rates, a population-based screening program reaching all women at risk should be instituted. Since it would take almost two decades to build up the infrastructure for a conventional approach, it has been decided to employ newly developed technology, which could be implemented in a couple of years [77].


### 3.9. Cervical Cancer Screening in Developing Countries

In Sub-Saharan Africa (SSA), although incidence rates vary from country to country, cervical cancer is the second most common cancer of reproductive-aged women [78]. In 2004, the Malawian Ministry of Health has introduced a comprehensive cervical cancer screening programme using visual inspection with acetic acid (VIA) and cryotherapy [79]; objective of the screening programme was to screen 80% of Malawi’s eligible women. Despite the programme has not achieved its goal, the percentage of women screened increased from 9.3% to 26.5%, but with major problems related to “lost to follow-up” of positive patients. In Nigeria, the most populous country in Africa, almost 15,000 new cases of cervical cancer are diagnosed annually [80]. Nigeria has developed a National Cancer Control Policy that incorporates implementation of HPV DNA testing/VIA with the treatment of precervical lesions and implementation of HPV vaccination programmes [80]. Currently, HPV vaccination is available on a private basis only, but efforts are ongoing to incorporate HPV vaccination into their routine immunization programmes. In 2013, it became the first African country to implement a national prevention programme for cervical cancer [81]. The Rwanda national HPV vaccination model could be the first step towards cervical cancer elimination in Africa [80].In the Middle East and North Africa, the first steps to implement national screening programmes based on visual inspection tests are being currently complete [82]. The cervical cancer screening coverage in Southern Africa ranges between 4.1 and 38.0% [83]. One preventive strategy used in South Africa was the introduction of the HPV vaccine. In 2014, a national school-based program for the HPV bivalent vaccine was introduced in all public schools, targeting girls in Grade 4 (aged ≥9 years old) with a two-dose (6 months apart) schedule [84]. In India, guidelines for population-based screening programmes for cervical cancer are available [85] and are based, largely, on visual inspection tests; however, screening coverage is still very low.Few population-based cervical cancer screening programs had been implemented in Western Asian and Middle Eastern Arab countries, with low awareness about cervical screening among Arab Muslim. Factors affecting the coverage of cervical cancer screening practices were the absence of organized screening programs, low screening knowledge among women, healthcare professionals’ attitudes toward screening, pain and embarrassment, stigma, and sociocultural beliefs [86].


## 3.1. Europe

In Europe, about 60,000 new cases of cervical cancer are being diagnosed every year, with 25,000 deaths per year [53]. Despite the implementation of vaccination programs, screening for cervical cancer precursors and treatment of detected lesions will remain the most effective tool for cervical cancer prevention in the medium-long term. Cervical cytology has been the cornerstone of European screening programs: according to data from the International Agency for Research on Cancer, Pap smears performed every 3–5 years reduce the incidence of cervical cancer by 80% [54]. Screening is “organized” in the Nordic countries, in the United Kingdom, in Holland, in Poland, Croatia and Slovenia, Belgium, and the Czech Republic, and in many parts of Italy [54, 55]; screening is “opportunistic” in the remaining countries (with a low cost-effectiveness ratio: overscreened women and underscreened women). Table 2 shows the programmes of cervical cancer screening and human papillomavirus (HPV) vaccination in European countries [55].Table 2
Programmes of cervical cancer screening and HPV vaccination in European countries.
CountriesCervical screening program (starting year)Screening ageScreening intervalPrimary test usedHPV vaccine (national vaccination program) (year of initiation)AustriaOpportunistic18+ or 2 years after sexual onset1CC2014BelgiumOrganized population-based, in some regions, rollout ongoing, 201325–643CC & LBC2010BulgariaOpportunisticNANANA-CroatiaOrganized population-based, rollout ongoing, 201225–643CC2016CyprusOpportunisticNANAN/A2016Czech republicOrganized population-based, rollout ongoing, 200815+1CC2012DenmarkOrganized population-based, 200623–643 (ages: 23–59); 5 (ages: 60–64)LBC (ages: 23–59); HPV primary test (ages:60–64)2009EstoniaOrganized population-based, 200630–595CC2018FinlandOrganized population-based (depending on the region), 196325–655CC, HPV primary testing in some regions2013FranceTransitioning to organized population-based planned, 199125–643 (CC), 5 (HPV test)CC & LBC (ages: 25–64); HPV primary testing in regional pilot projects (ages: 30–64)2007GermanyTransitioning to organized population-based planned, 197120+1CC, HPV primary testing in implementation2007GreeceOpportunisticSexual onset1CC2008HungaryOrganized population-based, rollout ongoing, 200325–653CC2014IrelandOrganized population-based, rollout ongoing, 200825–603 (ages: 25–44); 5 (ages: 45–60)LBC2010ItalyOrganized population-based, rollout ongoing, depending on the region, 198925–643 (ages: 25–30/35); 5 (ages: 30/35–64)CC & LBC (ages: 25–30/35); HPV primary testing in some regions (ages: 30/34–64)2008LatviaOrganized population-based, 200925–693CC2010LithuaniaOrganized population-based, rollout ongoing, 200425–593CC2016LuxembourgOpportunistic15+1LBC2008MaltaOrganized population-based, piloting, 201525–353CC2012NetherlandsOrganized population-based, 197030–645HPV primary testing2010NorwayOrganized population-based, 200625–693CC & LBC, regional pilot for HPV primary testing,2009PolandOrganized population-based, 200625–593CC (ages: 25–59); regional pilot for cotesting (ages: 30–59)—PortugalOrganized population-based, in some regions, rollout ongoing, depending on the region, 199025–593Cotesting in some regions2008RomaniaOrganized population-based, in some regions, rollout ongoing, 201225–645Cotesting in some regions—SlovakiaTransitioning to organized population-based, 200823–64Yearly x 2; then 3 yearlyCC—SloveniaOrganized population-based, (2003)20–64Yearly x 2; then 3 yearlyCC2009SpainOpportunistic (depending on the region)25–653CC2007SwedenOrganized population-based, 196723–603 (ages: 23–50); 5 (ages: 51–60)HPV test replacing CC & LBC2012SwitzerlandOpportunistic21/702 (ages: sexual onset/21–29); 3 (ages: 30–70)CC & LBC2008The United KingdomOrganized population-based, 198825–643 (ages: 25–49); 5 (ages: 50–64)HPV primary testing in implementation2008CC = conventional cytology; LBC = liquid-based cytology; N/A: not available.The Council of the European Union (which represents the 27 member states) has always recommended the cervical cancer screening intervals reported in Table3 [56].Table 3
Cervical cancer screening intervals with the Pap test according to the European Union recommendation (depending on the available resources in each member state).
AgeMethod and intervalsOnset of screening: between 20-25 and 30 yearsPap test every 3–5 yearsEnd of screening: 60–65 yearsA supplement to the second edition of the European guidelines [6] was published in September 2015 introducing the HPV DNA test alone, as the primary screening test in European countries (as opposed to the American guidelines, which recommended cotesting), together with the implementation of HPV vaccination programs. Notably, it was reported that(1)
the primary test for the detection of high-risk oncogenic HPV types can be used within an organized screening program,(2)
HPV test is not recommended outside of organized programs. Cotesting (the Pap test and HPV test) should be avoided: only one primary test, Pap test or HPV test, should be employed,(3)
routine screening with HPV tests should be used starting at the age of 35 and in any case not below 30 years (in the age group between 30 and 34 years, the available evidence was not considered sufficient to recommend HPV DNA testing). Under 30–34 years of age, the recommended screening test is the Pap test,(4)
the screening interval in women with the HPV negative test must be at least 5 years, with the possibility of extension up to 10 years, depending on the age and history of the patient. Screening can cease at the age of 60–65, provided that the woman has a recent negative test, and(5)
the management of a woman with a positive HPV test involves triage with cervical cytology, while direct referral to colposcopy is not recommended.Table4 reports cervical cancer screening intervals with the HPV test and Pap test, according to European Union recommendations.Table 4
Cervical cancer screening intervals with the HPV test and pap test, according to the European union recommendation (depending on the available resources in each member state).
AgeMethod and intervalsScreening starts between 20-25 and 30 yearsPap test every 3–5 yearsAn alternative starting at 30–35 yearsHPV DNA test every 5–10 yearsEnd of screening: 60–65 yearsFurthermore, the European Council recommends high quality standards in all phases of the program (invitation, screening, diagnosis, treatment of detected lesions, and post-treatment follow-up), and urges to discourage opportunistic screening as well as to refrain from introducing new primary screening programs in the absence of population studies [6, 56].Despite the consistent evidence that organized programs are more efficient than opportunistic screening, and despite the recommendations of the European Council, the health authorities of many European countries have not yet implemented a nationally organized cervical cancer screening program [57, 58]. The Italian health system, by means of all the regions in the country, is committed to changing the screening program by the end of 2020. However, a great variability still exists from region to region [59], with local realities employing cervical cytology every 3 years from 25 to 64 years of age, and regions employing cytology from 25 to 29 years and then shifting to HPV testing every 5 years from 30 to 64, and situations in which screening is spontaneous and unorganized, with differences even within a same region. On the other hand, in Holland, the HPV DNA test program was established at a national level, at 5-year intervals from 30 to 40 years, and at 10-year intervals from 40 to 60 years. No screening is recommended for women under 30 and over 64 years [60].In the United Kingdom, cervical screening follows the European Guidelines, but liquid-based cytology is recommended instead of conventional cytology (Table5). HPV screening is being piloted across England to assess how this approach can be used across the programme as a whole [61, 62].Table 5
Cervical cancer screening intervals with the Pap test, according to the United Kingdom recommendation.
AgeMethod and intervals<25 yearsNo screening25–49 yearsLiquid-based cytology every 3 years50–64 yearsLiquid-based cytology every 5 years≥65 yearsScreening for women who have had recent abnormal tests or who have not had an adequate screening test reported since age 50


## 3.2. The Russian Federation

Cervical cancer is the second cause of death in women in Russia. About 70% of all cancers are still diagnosed in advanced stages. No organized screening program is in place, but the Minister of Health recommends screening as for the WHO and European standards [63].


## 3.3. The United States of America

The American guidelines are the most comprehensive, undoubtedly validated by sufficient scientific evidence in their effort to achieve the maximum cost-effectiveness ratio, but certainly complex in practical management. Recommendations have a “grading” with 5 degrees (“A” when the benefit is high, “B” when it is moderate, “C” when it is low, “D” when the disadvantages outweigh the advantages, and “I” when the scientific evidence is not yet sufficient to determine the balance between benefits and harms), and quality of evidence at 3 levels (“high,” “moderate,” and “low”). The recommendations were shared, until July 2020, by the American Cancer Society (ACS), the American Society for Colposcopy and Cervical Pathology (ASCCP), and the American Society for Clinical Pathology (ASCP) (Table6) [7, 8]. In July 2020, the ACS produced a guideline update that leads to substantial changes [10]. Until July 2020, the ACS was in accordance with other American societies.Table 6
Cervical cancer screening intervals with the HPV test and Pap test, according to the American Society for Colposcopy and Cervical Pathology (ASCCP) and the American Society for Clinical Pathology (ASCP).
AgeMethods<21 years (Grade D)No screening (in the case of a Pap smear ASC-US: HPV testing is not recommended)21–29 years (Grade A)Pap smear every 3 years30–65 years for women who want to extend their screening interval (Grade A)Cotest (the Pap test + HPV DNA test) every 5 years30–65 years in women who choose to be tested more often (Grade A)Pap smear every 3 years>65 years (Grade D)No screening in women with previous adequate screening (i.e., at least two negative results in the last 10 years, with at least one in the last 5) in women with a history of CIN 2 or worse lesions: Continue routine screening for at least 20 yearsScreening posthysterectomy (Grade D)No screening (women with removal of the cervix and no history of a high-grade precancerous lesion in the last 20 years, or cervical cancer ever)After HPV vaccination (Grade C)Age-based recommendations for the general population (at the moment according to the same guidelines as women who have not been vaccinated)The most recent ACS guideline (July 2020) [10] calls for substantial changes to the screening method: the ACS recommends that individuals with a cervix initiate cervical cancer screening at age 25 y and undergo primary HPV testing every 5 y through age 65 y (preferred). If primary HPV testing is not available, individuals aged 25–65 y should be screened with cotesting (HPV testing in combination with cytology) every 5 y or cytology alone every 3 y (acceptable) (strong recommendation). A cotesting or cytology testing alone are included as acceptable options for cervical cancer screening because access to primary HPV testing with a test approved by the FDA for primary screening may be limited in some settings. The guideline is transitional; that is, options for screening with cotesting or cytology alone are provided but should be phased out once full access to primary HPV testing for cervical cancer screening is available without barriers [10].The U.S. Preventive Services Task Force (USPSTF), probably the most rigorous in the formulation of the guidelines, prepared a separate document in which women aged 30 to 65 years should be screened every 3 years with cervical cytology alone, or every 5 years with high-risk HPV testing alone or in combination with cytology [11].The cotest (the Pap test + HPV test) is only offered in women aged 30 to 65 years, provided that the 5-year rescreening interval is respected. This approach is defined “preferable” for the ASCCP/ASCP, while it is defined “acceptable” for the ACS/USPSTF. HPV testing as a standalone test (i.e., without cytology) was not recommended.A study [64] was conducted to verify the ideal strategy for cervical cancer screening, which should offer the maximum sensitivity to minimize the risk of missing the disease, together with the maximum specificity to reduce the rate of false-positive cases. This analysis involved 34,254 women aged ≥30 years, extrapolated from the ATHENA (Addressing the Need for Advanced HPV Diagnostics) trial, with an average age of 44.7 years. 8.4% of women were HPV positive, and 5.7% had an abnormal Pap smear. Ten different screening strategies were compared. The most attractive for the “benefits” versus “harms” analysis was the four strategies that involved the use of the HPV test with genotyping for HPV 16/18 (two of which in the cotest with cytology). The two strategies that would seem to optimize the balance between sensitivity and specificity are the cotest with genotyping and triage with Pap LSIL (strategy no. 5) and the HPV test with genotyping and triage with Pap ASC-US (strategy no. 9). The latter led to a 50% reduction in the number of screening tests required, as well as being slightly more sensitive and requiring slightly less colposcopies to detect a CIN 3 or worse lesion. In conclusion [64], according to the authors, to employ in primary screening, a strategy involving the use of the HPV test with triage of HPV-positive women through a combination of genotyping for HPV 16/18 and cytology would provide a good balance between obtaining of maximum sensitivity and specificity, limiting the number of colposcopy referrals. However, it must be noted that the study in question is a post hoc analysis (the ATHENA study had another objective); moreover, it was impossible to know how many high-grade lesions or worse would have been diagnosed at the next screening or during the follow-up.Last but not least, compliance with recommendations to follow-up is essential; otherwise, the assumptions of the entire algorithm collapse. These strategies are therefore a little too intricated, difficult to apply in real practice as far as regards doctors’ attitudes as well as patients’ preferences. The American algorithms are also very complex for professionals and, in their attempt to maintain a correct cost-effectiveness ratio, they risk being difficult to use and above all not being respected [7–9, 18, 19, 65]. For instance, the most common rescreening interval after a negative HPV result was found to be only 12 months in the United States [60]. Interim Clinical Guidance for use of primary high-risk human papillomavirus testing for cervical cancer screening has also been published introducing in the USA the use of the HPV test as the only primary screening method [3, 66]. However, these works are burdened by heavy conflicts of interest (Merck, GSK, Hologic, Roche, Gen Probe, Becton-Dickinson, Cepheid, and Genentech). In Wright’s article, even one of the sponsors (Roche Molecular Systems, Pleasanton, CA) was involved in all aspects of the design and conduct of the study: collection, management, analysis, and interpretation of the data; two employees of the pharmaceutical company were integral to the preparation of the manuscript, and the sponsor reviewed the final version of the manuscript [3].In a conflict-free article [67], however, the Cobas test was evaluated on more than 33,000 Pap smears, detecting a 59.7% negative test rate in cases with abnormal cytology and a 38.2% negative test rate in a subset of histologically confirmed CIN 2–3 lesions. Obviously, the authors’ conclusion is that the HPV Cobas test cannot be used in primary screening, as it would provide a false guarantee to doctors and patients, delaying appropriate treatment.The latest recommendations from the Atlanta Centers for Disease Control and Prevention (CDC) (June 2015) clearly stated that the HPV test should not be recommended for screening for cervical cancer as a standalone test (i.e., without a concurrent Pap test) [68].Finally, in 2020, the ASCCP has introduced new management guidelines for cervical cancer screening abnormalities, revolutionizing the future of risk-driven recommendations for this topic (see below) [69].


## 3.4. Canada

In 2011, around 1.300 new cases of cervical cancer were recorded in Canada (with about 350 deaths). There has been no reduction in cervical cancer mortality in Canada since 1970 in women aged 20–24 years (i.e., since the start of screening) [70].The recommendations follow a simpler “grading” than the USA, with only 2 degrees: “strong” or “weak”; on the other side, the quality of evidence is divided into 4 levels: “high,” “moderate,” “low,” and “very low.” Three-year intervals are the right balance between the small potential to increase the benefits obtainable with shorter intervals and the greater potential for harms resulting from the increase in tests and procedures related to more frequent screening. Screening by the HPV test, alone or in combination with the Pap test, is not recommended (as there was insufficient evidence on its effect on mortality and incidence of invasive cancer). Canadian guidelines also state that organized screening is more effective than opportunistic screening. The sensitivity and specificity of thin-layer and conventional cytology are similar. An increase or decrease in screening may be appropriate for women with different risk profiles. The recommendations are reported in Table7.Table 7
Cervical cancer screening intervals according to the Canadian task force on preventive health care recommendations on screening for cervical cancer.
AgeMethodGrade of recommendation/quality of evidence<20 yearsNo screeningStrong/high20–24 yearsNo screeningWeak/moderate25–29 yearsPap smear every 3 yearsWeak/moderate30–69 yearsPap smear every 3 yearsStrong/high>70 a yearsNo screening if at least three consecutive negative Pap tests in the past 10 years; otherwise, continue screening until three consecutive negative resultsWeak/lowThese recommendations do not apply to women with cervical cancer symptoms or previous abnormal screening test results; women whose uterine cervix has been completely removed; immunosuppressed women for HIV, organ transplants, chemotherapy, or chronic use of corticosteroids; and women with reduced life expectancy so that they cannot benefit from screening. Finally, it is recommended that clinicians be aware of the values, preferences, and beliefs of each woman. HPV DNA testing as primary screening strategy is being introduced in many provinces.


## 3.5. Latin America

Screening programs for cervical cancer in Latin America and the Caribbean had not been successful in many situations, and incidence and mortality are still high. Even after the introduction of screening programs, incidence and mortality for cervical cancer did not change in many countries, because the coverage of screening is inadequate in rural areas [71, 72]. Of note, Chile, Colombia, Mexico, Peru, and more recently Argentina, are actively evaluating and proposing new strategies for cervical cancer control [73]. In consequence, countries such as Chile, Colombia, and Costa Rica are registering reductions in mortality rates for cervical cancer [72].


## 3.6. Australia

Until 2017, Australian recommendations were very simple and effective. The first Pap smear was proposed between 18 and 20 years of age, or 1–2 years after the onset of sexual activity (depending on which of the two events occurs later) and then repeated every 2 years until 69 years.The “National Cervical Screening Program” was revised in 2017 [74]. Therefore, starting from this date, HPV testing was introduced for primary screening (Table 8).Table 8
Cervical cancer screening intervals according to the Australian guidelines.
AgeMethod and intervals25–74 yearsHPV DNA every 5 years>74 yearsNo screening (at the discretion of the caregiver, if the previous results are normal)The new cervical cancer screening pathway is a risk-based approach in which partial genotyping is used to classify the type of HPV into one of two groups: oncogenic HPV 16/18 or oncogenic HPV types not 16/18, as a pooled result. The Australian risk-based approach, according to HPV test result, is reported in Table9.Table 9
Cervical screening test results for clinician-collected sample according to the Australian guidelines.
Risk of significant cervical abnormalitiesHPV test resultReflex liquid-based cytology resultRecommended managementLow-risk resultHPV not detected—Return to screening in 5 yearsIntermediate-risk resultHPV not 16/18 detectedNegative, possible LSIL or LSILRepeat HPV test in 12 monthsHigher-risk resultHPV not 16/18 detectedPossible HSIL or HSILRefer to specialist (colposcopy)HPV 16/18 detectedAny LBC resultRefer to specialist (colposcopy)—Unsatisfactory HPV test—Collect new sample for the HPV test in 6–12 weeks—HPV not 16/18 detectedUnsatisfactoryCollect new sample for LBC only in 6–12 weeksCotesting is recommended for symptomatic patients (usually abnormal vaginal bleeding) requiring investigation, patients exposed to diethylstilbestrol (DES), patients undergoing Test of Cure (TOC) surveillance, and patients who have been treated for glandular abnormalities.


## 3.7. Japan

Cervical cancer is the ninth leading cause of cancer death in women in Japan. In 2018, about 13.277 new cervical cancer cases were diagnosed in Japan. Recommendations have a 5-degree “grading” as in the USA (“A” and “B” grades indicate when recommendations are valid for both mass and opportunistic screening programs, “C” when they are not valid for mass screening, “D” when they are neither valid for mass nor opportunistic screening, and “I” refers to screening methods in which there is insufficient evidence), and quality of evidence up to 8 levels (based on the type of study: design, quality, and consistency of the study) [75]. Screening guidelines were formulated on the basis of the balance between benefits and harms recommendations for population-based and opportunistic. Five cervical cancer screening methods were evaluated (three of them with the HPV test), after considering 3.450 articles published from January 1985 to October 2007. After a systematic review of the literature, 161 articles were selected and 66 were confirmed. The results of 33 studies were considered satisfactory and the scientific evidence sufficient to evaluate the effect of screening by conventional cytology. The accuracy of thin-layer cytology was equivalent to that of conventional cytology. Although HPV tests and combination methods have shown high sensitivity, no studies have evaluated the reduction in mortality from cervical cancer. In mass screening, as well as in opportunistic screening, conventional or thin-layer cytology is recommended, given sufficient scientific evidence (Grade B). Cervical cancer screening using the HPV test alone or in combination with cervical cytology was not recommended in mass screening, given insufficient scientific evidence (Grade I). Since 2003, screening in Japan has been carried out, as reported in Table 10.Table 10
Cervical cancer screening intervals according to the Japanese guidelines.
AgeMethod and intervals>20 yearsPap test every 2 yearsGiven the different health system in Japan compared to other countries, it was not considered appropriate to modify the current recommendations. To reduce cervical cancer mortality in Japan, it is rather necessary to improve adherence to screening programs and the appropriateness of managing the lesions detected [75]. At the same time, a retrospective study on 3.804 women aged ≥20 years followed for 5 years (from 2005 to 2010) [76], assessed how the incidence rates of cytological abnormalities, CIN, and cervical cancer vary according to age. Under the age of 40, about 5% of women had an abnormal Pap test result, about 3% had CIN, and 0.5% developed a CIN within two years. In women aged between 40 and 49, less than 4% had a cytological abnormality within two years and approximately 5% within three years. In the group between 50 and 59 years old, less than 2% had a cytological abnormality within two years and about 3% within three years. While 0.1% of women developed a CIN in the 40–49 year group within three years, none in the 50–59 year group developed a CIN within three years. In women aged 60 and over, less than 3% had an abnormal Pap test result, less than 0.5% developed a CIN within 5 years, and none developed cancer in the same period. According to the results of this study, the author proposed the following modification [76]: Pap test every 2 years between 20 and 40 years, every 2 or 3 years between 40 and 59 years, and every 5 years after 60 years.


## 3.8. China

Although in China today there are several excellent programs for cervical cancer screening in large cities, these programs cover only a minimal part of the whole population in the country. To achieve a significant reduction of the incidence of invasive cervical cancer with concomitant reduction in the death rates, a population-based screening program reaching all women at risk should be instituted. Since it would take almost two decades to build up the infrastructure for a conventional approach, it has been decided to employ newly developed technology, which could be implemented in a couple of years [77].


## 3.9. Cervical Cancer Screening in Developing Countries

In Sub-Saharan Africa (SSA), although incidence rates vary from country to country, cervical cancer is the second most common cancer of reproductive-aged women [78]. In 2004, the Malawian Ministry of Health has introduced a comprehensive cervical cancer screening programme using visual inspection with acetic acid (VIA) and cryotherapy [79]; objective of the screening programme was to screen 80% of Malawi’s eligible women. Despite the programme has not achieved its goal, the percentage of women screened increased from 9.3% to 26.5%, but with major problems related to “lost to follow-up” of positive patients. In Nigeria, the most populous country in Africa, almost 15,000 new cases of cervical cancer are diagnosed annually [80]. Nigeria has developed a National Cancer Control Policy that incorporates implementation of HPV DNA testing/VIA with the treatment of precervical lesions and implementation of HPV vaccination programmes [80]. Currently, HPV vaccination is available on a private basis only, but efforts are ongoing to incorporate HPV vaccination into their routine immunization programmes. In 2013, it became the first African country to implement a national prevention programme for cervical cancer [81]. The Rwanda national HPV vaccination model could be the first step towards cervical cancer elimination in Africa [80].In the Middle East and North Africa, the first steps to implement national screening programmes based on visual inspection tests are being currently complete [82]. The cervical cancer screening coverage in Southern Africa ranges between 4.1 and 38.0% [83]. One preventive strategy used in South Africa was the introduction of the HPV vaccine. In 2014, a national school-based program for the HPV bivalent vaccine was introduced in all public schools, targeting girls in Grade 4 (aged ≥9 years old) with a two-dose (6 months apart) schedule [84]. In India, guidelines for population-based screening programmes for cervical cancer are available [85] and are based, largely, on visual inspection tests; however, screening coverage is still very low.Few population-based cervical cancer screening programs had been implemented in Western Asian and Middle Eastern Arab countries, with low awareness about cervical screening among Arab Muslim. Factors affecting the coverage of cervical cancer screening practices were the absence of organized screening programs, low screening knowledge among women, healthcare professionals’ attitudes toward screening, pain and embarrassment, stigma, and sociocultural beliefs [86].


## 4. The New Risk-Based Guidelines for Cervical Cancer Screening Abnormalities, in the Postvaccination Era

Guidelines are being developed with the aim of reducing the number of tests a woman needs during her lifetime, in order to receive the maximum benefit from screening, while decreasing potential harms that may result with the use of a screening strategy (overdiagnosis, overtreatment, anxiety, and costs). With this purpose in mind, the ASCCP has recently introduced new management guidelines for cervical cancer screening abnormalities, where recommendations are based on risks, not results [69, 87–89].The American guidelines for the management of the altered screening test are an evolution also with respect to the innovative Australian guidelines [74].This change from primarily test results to primarily risk-driven guidelines was made possible through careful evaluation of specific clinical action thresholds. HPV DNA testing is the basis for risk estimation, with reflex cytology as a triage test for all HPV-positive results. Surveillance thresholds work on the principle of similar management for similar risks. Thus, a set of probabilistic risk models were studied within a minimum amount of data available, to provide a personalized estimate of risk for the high-grade cervical disease over time. Depending on age (<25 years and ≥25 years), current test results (cytology, HPV testing or both), and previous screening history when available, five different scenarios could be identified [66]:(1)
Return to routine screening (preferably HPV-based), when the 5-year risk of a high-grade cervical lesion is less than 0.15%(2)
Surveillance with repeat testing in 3 years without colposcopy, when the 5-year risk ranges from 0.15% to 0.54%(3)
Surveillance with repeat testing in 1 year without colposcopy, when the 5-year risk ranges from 0.55% to 3.9%(4)
Colposcopy, when there is an immediate risk of high-grade disease ranging between 4% and 24%(5)
Treatment, when the immediate risk of high-grade disease is ≥ 25% (with the option of expedited treatment, i.e., not preceded by a confirmation biopsy, when the risk is ≥ 60%)This shift represents a great leap forward to precision medicine, where risks are individualized and care is person-centered.The risk of progression to severe high-grade CIN and cancer is strongly associated with HPV genotype and genotype-specific persistence. Different oncogenic genotypes entail different risks, with HPV 16 ranking the highest, and HPV 66 the lowest [90]. Extended genotyping (i.e., assays reporting all 14 high-risk genotypes) enables personalized clinical management for women screened through the primary HPV paradigm. By stratifying genotype-specific results, it is possible to assign women to different interventions. Highest risk genotypes (HPV 16, 18, 31, and 33) together with moderate risk genotypes (HPV 45, 52, and 58) should be managed differently than the types at lower risk (HPV 35, 39, 51, 56, 59, 66, and 68), which could be followed up less stringently. Since guidelines are intentionally built to allow updates to incorporate new tests (e.g., full HPV genotyping or dual staining with p16/Ki-67 immunocytology), as well as to adjust for decreasing incidence of high-grade lesions following HPV vaccination [66], it can be foreseen a prolonged rescreening interval among the vaccinated population. Quadrivalent HPV vaccine is available since 2006, while nonavalent HPV vaccine since 2016. Women vaccinated with the latter are to be considered protected right against the seven abovementioned HPV genotypes at higher risk. The information obtained can be easily introduced into the data required to retailor risk estimates, though algorithms would certainly result even more intricate. To overcome the challenge, national societies are planning to implement algorithms on mobile phones. Apps are already available to assist with the navigation of previous guidelines [91] and a new one has been recently developed by ASCCP. These apps could be upgraded in real time, as new mosaic tiles will become available once validated by clinical trials. In a world where telemedicine and telemonitoring are at hand [92], not only as a support in underserved communities but also when there is a pandemic or other types of crisis, the opportunity to dispose of tech tools to guide health providers through all steps of a complex screening algorithm will be much appreciated.In conclusion, full genotyping can resize the risk on the basis of specific high-risk genotypes. In the same way, the data regarding HPV vaccination could be introduced as soon as women vaccinated with the nonavalent vaccine reach the screening age, with the recommendation of a prolonged screening interval. Lifetime testing could be cut in half among the vaccinated population, reducing costs, anxiety, and possible overtreatments. Nevertheless, clinicians should always bear in mind that screening focuses on people with no symptoms and that in the presence of risk factors (postcoital bleeding, cigarette smoking, immunosuppression, and unexplained vaginal discharge), women should follow a different pathway, and further investigation may be requested regardless of test results.



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*Source: 8887672-2020-11-05.xml*