# An Efficient and Mild Method for the Synthesis and Hydrazinolysis ofN-Glyoxylamino Acid Esters

Authors: Ayman El-Faham; Zainab Al Marhoon; Ahmed Abdel-Megeed; Mohamed Siddiqui
Journal: Journal of Chemistry
 (2013)

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## Abstract

N-Glyoxylamino acid ester derivatives were synthesized via the ring opening of N-acetylisatin using moderate conditions. During the hydrazinolysis of N-glyoxylamino acid ester derivatives with hydrazine hydrate (80%) in methanol, unexpected reduction of the α-keto group occurred to afford N-acylamino acid hydrazide derivatives in good yield (80–90%) (Wolff-Kishner type reaction). All the synthesized compounds were characterized by 1H NMR, 13C NMR, and elemental microanalysis.


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## Body

## 1. Introduction

The glyoxylamides are compounds of widespread interest in organic chemistry and present in many biologically active compounds [1–8]. In parallel with the application of the glyoxylamide in medicinal chemistry, numerous synthetic methods have been described in the literature [9–26]. Other authors have also reported that synthesis of the glyoxylamide fragment could be achieved by the ring opening of N-acetylisatin 1by attacking an amine at C2-carbonyl group of N-acetylisatin (Scheme 1) [27–35].Scheme 1
Formation of glyoxylamide fromN-acylisatin.Recently, Cheah et al. [35] reported the synthesis of N-glyoxylamide peptide mimics from the reaction of N-acetylisatin with L-α-amino esters. The reaction was carried in two-phase solvent system, DCM/H2O (2 : 1) in the presence of saturated NaHCO3 with yields ranging from 61 to 98%. They claimed that the low yield in some cases is due to the formation of glyoxalic acid derivative (Figure 1).Figure 1
Structure of glycoxalic acid derivative.In the present work, we reported the synthesis of some newN-glyoxylamino acid ester using the reported method by Popp and Piccirilli [28] and Obafemi et al. [29] where acetonitrile and K2CO3 were used instead of DCM-H2O/NaHCO3. The products N-glyoxylamino acid ester were used as precursors for the synthesis of their hydrazide derivatives with reduction of the α-keto amide group under very mild conditions.


## 2. Results and Discussion

Ethyl-4-aminobenzoate was selected as a first model to react withN-acetylisatin using methanol as a solvent to afford the expected product 2 in yield 87% (Scheme 2). Compound 2 was dissolved in methanol and hydrazine hydrate (80%) was added dropwise at room temperature; the reaction mixture was stirred at the same temperature overnight. The white precipitate formed during the reaction was filtered and dried to afford the product 4 in pure state as indicated from its spectral data. IR for compound 2 showed the carbonyl groups at 1746.09 (CO-ester), 1694.361 (α-CO), 1654.00, and 1603.36 (CONH) cm−1, while the IR spectra of 4 showed only the carbonyl group at 1676.44, 1611.89, and 1563.43 (CONH) cm−1 with the disappearance of the α-keto group at 1694 cm−1.Scheme 2
Proposed mechanism for the hydrazinolysis of glyoxylamide ester.The1H NMR of 4, also confirmed the structure, where a singlet peak was observed at δ 4.48 corresponding to the methylene group. The 13C NMR of 4also confirmed its structure, where the α-keto group at δ 185.61 ppm was not observed but instead a methylene group was observed at δ 87.45 ppm. The data obtained from the IR and NMR spectral analysis proved the suggested mechanism illustrated in Scheme 2. During the hydrazinolysis of 2 with hydrazine hydrate (80%), compound 3 was formed and then undergoes reduction due to the presence of excess of hydrazine [36–39] (Wolff-Kishner type reaction) to afford the product 4 instead of the ketoamide hydrazide derivative 5 (Scheme 2).In the light of the reaction conditions described in Scheme2, the same reaction was carried out using methyl-2-aminobenzoate 6; after removing of the solvent, yellow crystals were formed. The spectral data of the product obtained agreed with the structure of 7(Scheme 3); where the IR spectra of 7 showed three carbonyl groups corresponding to α-ketoester (COCOOCH3) and amide group (NHCOCH3) at 1746.66, 1695.87, and 1605.65 cm−1, respectively. The 1H NMR confirmed the structure of 7, where two singlet peak were observed at δ = 2.25 and 3.99 ppm corresponding to the methyl group NHCOCH3 and COOCH3, respectively. The 13C NMR also confirmed the structure of 7, where two singlet peak were observed at δ = 25.60, 53.06, and 190.32 ppm corresponding to (NHCOCH3), (COOCH3), and (C6H4COCO–), respectively, with other peaks related to the rest of carbons skeleton.Scheme 3
Reaction ofN-acetylisatin with methyl-2-aminobenzoate.The reaction of1 with methyl-2-aminobenzoate was repeated in acetonitrile instead of methanol, and only about 8–10% yield of 8 was formed even after reflux in acetonitrile for 12 h as observed for the NMR data. These results might be due the steric hindrance of methyl-2-aminobenzoate; N-acetylisatin 1 undergoes ring opening in presence of methanol to afford 7as a major product (Scheme 3).The reaction ofN-acetylisatin 1was extended to react with other amino acid ester hydrochloride 9a–d[40, 41]. The reaction was performed in CH3CN and K2CO3 at rt to afford the products 10a–d in 80–92% yield (Scheme 4, Table 1). The products 10a–d were subject to react with hydrazine hydrate under the same conditioned described in Scheme 3 to afford products 11a–d in 80–92% yield (Scheme 4, Table 1). The structures 10a–dand 11a–d were confirmed by IR, NMR (1H NMR and 13C NMR), and elemental analysis.Table 1
Yield %, Mp (°C) and Elemental Analysis of10a–d and 11a–d.
Cpd no.
Yield (%)
Mp (°C)
Elemental analysis calcd (found)
C
H
N
10a
89
108–110
57.53 (57.85)
5.52 (5.81)
9.58 (9.33)
10b
87
90–92
57.53 (57.28)
5.52 (5.81)
9.58 (9.33)
10c
90
100–102
58.82 (59.03)
5.92 (5.81)
9.15 (8.89)
10d
92
92–94
61.07 (61.23)
6.63 (6.79)
8.38 (8.57)
11a
80
198–200
54.54 (54.88)
6.10 (6.43)
21.20 (21.50)
11b
86
204–206
56.10 (55.87)
6.52 (6.81)
20.13 (19.89)
11c
84
195–197
57.52 (57.33)
6.90 (7.11)
19.17 (19.42)
11d
90
186–188
59.98 (60.11)
7.55 (7.67)
17.49 (17.68)Scheme 4
Reaction ofN-acetylisatin with amino acid ester. HCl and hydrazinolysis.


## 3. Conclusions

The acetonitrile/K2CO3 method avoids the formation of the glyoxylic acid formation during the synthesis ofN-glyoxylamino acid ester from the ring opening of N-acetylisatin. The hydrazine hydrate-methanol is an efficient and mild one-pot synthetic method for the preparation of N-phenyl acylamino acid hydrazide derivatives from their corresponding N-phenylglyoxylamino acid ester derivatives with reduction of the α-keto group (Wolff-Kishner reaction under mild condition) in an excellent yield.


## 4. Experimental Section

### 4.1. Materials

The solvents used were of HPLC reagent grade. Melting points were determined with a Mel-Temp apparatus and are uncorrected. Magnetic resonance spectra (1H NMR and 13C NMR spectra) were recorded on a Joel 400 MHz spectrometer with chemical shift values reported in δ units (ppm) relative to an internal standard. Elemental analyses were performed on Perkin-Elmer 2400 elemental analyzer, and the values found were within ±0.3% of the theoretical values. Followup of the reactions and checks of the purity of the compounds was done by TLC on silica gel-protected aluminum sheets (Type 60 GF254, Merck) and the spots were detected by exposure to UV-lamp at λ 254 nm for few seconds. The compounds were named using Chem. Draw Ultra version 11, Cambridge soft Corporation.


### 4.2. General Method for the Reaction ofN-Acetylisatin with Aminobenzoic Ester Derivatives

To the solution ofN-acetylisatin 1 (1.89 g, 10 mmol) in methanol (50 mL), which was heated up to 40–50°C, aminobenzoic ester (1.65 g, 10 mmol) was added with intensive stirring. The reaction mixture was refluxed with stirring for 2 h and then cooled down to room temperature. On the next day, the crystalline compound was collected with suction filtration, washed with little cold methanol, and dried under vacuum to afford the pure product.


#### 4.2.1. Ethyl 4-(2-(2-Acetamidophenyl)-2-oxoacetamido)benzoate (2)

The product was obtained as a yellowish white solid from ethanol (mp 78–80°C) in 87% yield. IR (KBr): 3424.75, 3222.27 (NH), 1746.09 (CO-ester), 1694.36 (α-CO), 1654.00, 1603.36 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.26 (t, 3H, CH3CH2), 2.03 (s, 3H, COCH3), 4.20 (q, 2H, COOCH2CH3), 6.57 (d, 2H, Ar), 7.29 (t, 1H, Ar), 7.56 (d, 1H, Ar), 7.63–7.65 (m, 5H, NH, Ar), 10.44 (s, 1H, NHCOCH3) ppm. 13C NMR (d6-DMSO): δ = 14.92, 23.95, 53.38, 113.25, 116.68, 122.96, 124.80, 126.46, 130.89, 131.57, 134.53, 137.89, 153.86, 163.14, 166.42, 169.65, 185.61 ppm. C19H18N2O5 (354.36): Calcd. C 64.40, H 5.12, N 7.91; found: C 64.60, H 5.33, N 7.76.


#### 4.2.2. Methyl 2-(2-Acetamidophenyl)-2-oxoacetate (7)

The product was obtained as yellow needles from methanol (mp 108–110°C) in 92% yield. IR (KBr): 3220.99 (NH), 1746.58 (CO-ester), 1696.22 (α-CO), 1656.79 (CONH) cm−1. 1H NMR (CDCl3): δ = 2.25 (s, 3H, COCH3), 3.99 (s, 3H, COOCH3), 7.14 (t, 1H, Ar), 7.64–7.68 (m, 2H, Ar), 8.78 (d, 1H, Ar), 11.06 (s, 1H, NH) ppm. 13C NMR (CDCl3): δ = 25.63, 53.08, 117.03, 120.76, 122.64, 133.63, 137.29, 142.77, 142.77, 163.95, 169.56, 190.34 ppm. C11H11NO4 (221.21): Calcd. C, 59.73; H, 5.01; N, 6.33; found: C 59.95, H 5.31, N 6.44.


### 4.3. General Method for the Synthesis of10a–d

To the solution ofN-acetylisatin 1 (1.89 g, 10 mmol) in acetonitrile (50 mL), amino acid ester hydrochloride [40, 41] (12 mmol) and K2CO3 (1.66 g, 12 mmol) were added with intensive stirring. The reaction mixture was stirred at room temperature overnight. On the next day, the reaction mixture was filtered with suction filtration and washed with 10 mL of acetonitrile. The solvent was removed under vacuum to dryness and the crude product was recrystallized from dichloromethane-hexane (1 : 3) to afford the pure product.


#### 4.3.1. Ethyl 2-(2-(2-Acetamidophenyl)-2-oxoacetamido)acetate (10a)

The product was obtained as off-white solid (mp 108–110°C) in 89% yield. IR (KBr): broad peak at 3263.71 for two (NH), 1747.33 (CO-ester), 1673.41 (α-CO), 1645.10, 1579.62 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.23 (t, 3H, CH2CH3), 2.10 (s, 3H, COCH3), 4.00 (d, 2H, NHCH2CO), 4.15 (q, 2H, COOCH2CH3), 7.25 (t, 1H, Ar), 7.65 (t, 1H, Ar), 7.76 (d, 1H, Ar), 7.97 (d, 1H, Ar), 9.17 (s, 1H, NH), 10.65 (s, 1H, NH) ppm. 13C NMR (d6-DMSO): δ = 14.65, 24.73, 40.28, 61.31, 121.31, 123.38, 123.81, 132.58, 135.08, 139.50, 165.00, 169.49, 169.65, 191.95 ppm. C14H16N2O5 (292.11): Calcd. C 57.53, H 5.52, N 9.58;    found C 57.85, H 5.81, N 9.33.


#### 4.3.2. Methyl 3-(2-(2-Acetamidophenyl)-2-oxoacetamido)propanoate (10b)

The product was obtained as off white solid (mp 90–92°C) in 87% yield. IR (KBr): 3287.56, 3124.00 (NH), 1740.76 (CO-ester), 1671.80 (α-CO), 1606.70, 1536.07 (CONH) cm−1. 1H NMR (CDCl3): δ = 2.18 (s, 3H, COCH3), 2.64 (t, 2H, CH2CH2CO), 3.67 (q, 2H, NHCH2), 3.70 (s, 3H, COOCH3), 7.09 (t, 1H, Ar), 7.45 (s, 1H, NH), 7.57 (t, 1H, Ar), 8.28 (d, 1H, Ar), 8.62 (d, 1H, Ar), 10.93 (s, 1H, NH). 13C NMR (CDCl3): δ = 25.52, 33.49, 35.06, 52.08, 118.57, 120.71, 122.58, 134.37, 136.62, 142.19, 163.05, 169.37, 172.61, 191.90 ppm. C14H16N2O5 (292.29): Calcd. C 57.53, H 5.52, N 9.58; found: C 57.28, H 5.81, N 9.33.


#### 4.3.3. Methyl 4-(2-(2-Acetamidophenyl)-2-oxoacetamido)butanoate (10c)

The product was obtained as off white solid (mp 100–102°C) in 90% yield. IR (KBr): 3272.48, 3123.79 (NH), 1739.37 (CO-ester), 1667.80 (α-CO), 1606.09, 1532.29 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.75 (m, 2H, CH2CH2CH2), 2.05 (s, 3H, COCH3), 2.39 (t, 2H, CH2CH2CO), 3.21 (q, 2H, HNCH2CH2), 3.60 (s, 3H, COOCH3), 7.24 (t, 1H, Ar), 7.60–7.63 (m, 2H, Ar), 7.87 (d, 1H, Ar), 8.75 (t, 1H, CH2NH), 10.60 (1H, s, NHCO). 13C NMR (d6-DMSO): δ = 24.45, 26.68, 29.56, 31.13, 38.37, 51.87, 122.02, 124.08, 124.79, 131.97, 134.43, 138.76, 164.11, 169.39, 173.63, 191.69 ppm. C15H18N2O5 (306.31): Calcd. C 58.82, H 5.92, N 9.15; found: C 59.03, H 5.81, N 8.89.


#### 4.3.4. Methyl 6-(2-(2-Acetamidophenyl)-2-oxoacetamido)hexanoate (10d)

The product was obtained as off white solid (mp 92–94°C) in 92% yield. IR (KBr): 3273.64, 3123.48 (NH), 1739.31 (CO-ester), 1667.77 (α-CO), 1606.05, 1532.23 (CONH) cm−1. 1H NMR (CDCl3): δ = 1.37–1.41 (m, 2H, CH2), 1.59–1.67 (m, 4H, 2CH2) 2.17 (s, 3H, COCH3), 2.31 (t, 2H, CH2), 3.37 (q, 2H, CH2), 3.64 (s, 3H, COOCH3), 7.03 (t, 1H, NHCH2), 7.09 (t, 1H, Ar), 7.54 (t, 1H, Ar), 8.26 (d, 1H, Ar), 8.57 (d, 1H, Ar), 10.93 (s, 1H, NHCO). 13C NMR (CDCl3): δ = 24.47, 25.50, 26.37, 29.00, 33.83, 39.39, 51.62, 118.76, 120.68, 122.64, 134.40, 136.51, 142.04, 163.17, 169.37, 174.01, 192.39 ppm. C17H22N2O5 (334.37): Calcd. C 61.07, H 6.63, N 8.38; found: C 61.23, H 6.79, N 8.57.


### 4.4. General Method for the Synthesis of (4 and 11a–d)

To the solution of glyoxyl derivatives (3 mmol) in methanol (15 mL), 0.7 mL of hydrazine hydrate (80%) was added. The reaction mixture was stirred at room temperature overnight. On the next day, the white precipitate was filtered with suction filtration and washed with cold 5 mL methanol (in case of there is no precipitation formed, the solvent was removed under vacuum and the crude product was washed with ether under stirring) to afford the product in pure state as observed from spectroscopic data.


#### 4.4.1.  2-(2-Acetamidophenyl)-N-(4-(hydrazinecarbonyl)phenyl)acetamide (4)

The product was obtained as a white solid (mp 186–188°C) in 82% yield. IR (KBr): 3420.52, 3338.27, 3340.34, 3209.27 (NH2 and NH amide), 1676.44, 1611.89 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.20 (s, 3H, COCH3), 4.33 (brs, 2H, 2NH), 4.48 (s, 2H, C6H4CH2CO), 6.97–6.99 (m, 4H, Ar), 7.08 (s, 1H, NH), 7.17–7.23 (m, 4H, Ar), 9.39 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.38, 87.45, 123.52, 124.08, 125.56, 126.57, 129.45, 142.01, 157.46, 168.93 ppm. C17H18N4O3 (326.35): Calcd. C 62.57, H 5.56, N 17.17; found C 62.80, H 5.74, N 17.43.


#### 4.4.2.  2-(2-Acetamidophenyl)-N-(2-hydrazinyl-2-oxoethyl)acetamide (11a)

The product was obtained as a white solid (mp 198–200°C) in 80% yield. IR (KBr): broad peak at 3275.61 (NH2 and NH amide), 1660.66, 1617.14, 1559.58 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.20 (s, 3H, COCH3), 3.69–3.79 (m, 2H, CH2), 4.24 (brs, 2H, 2NH), 4.71 (s, 2H, C6H4CH2CO), 6.98 (d, 1H, Ar), 7.16–7.24 (m, 3H, Ar), 8.48 (t, 1H, NH), 9.05 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.33, 41.59, 87.22, 123.68, 124.11, 125.49, 126.63, 129.43, 141.95, 157.40, 168.73, 170.72 ppm. C12H16N4O3 (264.28): Calcd. C 54.54, H 6.10, N 21.20; found: C 54.88, H 6.43, N 21.50.


#### 4.4.3.  2-(2-Acetamidophenyl)-N-(3-hydrazinyl-3-oxopropyl)acetamide (11b)

The product was obtained as a white solid (mp 204–206°C) in 86% yield. IR (KBr): 3432.09, 3366.21, 3320.90, 3222.65 (NH2 and NH amide), 1666.91, 1593.01, 1559.48 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.17 (s, 3H, COCH3), 2.30 (t, 2H, CH2CH2CO), 3.36 (q, 2H, NHCH2CH2), 4.19 (brs, 2H, NH), 4.51 (s, 2H, C6H4CH2CO), 7.00 (m, 2H, Ar), 7.14 (d, 1H, Ar), 7.24 (t, 1H, Ar), 7.28 (s, 1H, NH), 8.33 (t, 1H, NH), 9.08 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.18, 33.58, 36.46, 87.35, 123.75, 123.97, 125.54, 126.31, 129.50, 141.78, 157.73, 169.85, 170.63 ppm. C13H18N4O3 (278.31): Calcd. C 56.10, H 6.52, N 20.13; found: C 55.87, H 6.81, N 19.89.


#### 4.4.4.  2-(2-Acetamidophenyl)-N-(4-hydrazinyl-4-oxobutyl)acetamide (11c)

The product was obtained as a white solid (mp 195–197°C) in 84% yield. IR (KBr): 3344.24, 3304.18, 3260.00, 3219.07 (NH2 and NH amide), 1662.11, 1588.61, 1563.41 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.69 (m, 2H, CH2CH2CH2), 2.04 (t, 2H, CH2CO), 2.18 (s, 3H, COCH3), 3.14 (q, 2H, CH2CH2NH), 4.16 (brs, 2H, NH), 4.51 (s, 2H, C6H4CH2CO), 6.97–7.01 (m, 2H, Ar), 7.15–7.24 (m, 3H, 1NH, Ar), 8.34 (t, 1H, NH), 8.97 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.27, 25.83, 31.71, 40.32, 87.44, 123.62, 124.07, 126.27, 129.44, 157.63, 169.96, 171.95 ppm. C14H20N4O3 (292.33): Calcd. C 57.52, H 6.90, N 19.17; found: C 57.33, H 7.11, N 19.42.


#### 4.4.5.  2-(2-Acetamidophenyl)-N-(6-hydrazinyl-6-oxohexyl)acetamide (11d)

The product was obtained as a white solid (mp 186–188°C) in 90% yield. IR (KBr): 3400.00, 3311.34, 3213.91 (br) (NH2 and NH amide), 1663.64, 1563.97, 1527.95 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.20–1.23 (m, 2H, CH2CH2CH2), 1.45–1.48 (m, 4H, CH2CH2CH2CH2), 2.00 (t, 2H, CH2CO), 2.17 (s, 3H, COCH3), 3.13 (q, 2H, CH2CH2NH), 4.15–4.45 (br, 2H, NH), 4.50 (s, 2H, C6H4CH2CO), 6.98–7.02 (m, 2H, Ar), 7.13–7.33 (m, 3H, NH, Ar), 8.30 (t, 1H, HNCH2), 8.96 (s, 1H, HNCOCH3). 13C NMR (d6-DMSO): δ = 22.16, 25.50, 26.59, 29.23, 33.92, 40.13, 87.43, 123.68, 123.97, 125.69, 126.26, 129.47, 141.81, 157.81, 169.80, 172.27 ppm. C16H24N4O3 (320.39): Calcd. C 59.98, H 7.55, N 17.49; found C 60.11, H 7.67, N 17.68.


## 4.1. Materials

The solvents used were of HPLC reagent grade. Melting points were determined with a Mel-Temp apparatus and are uncorrected. Magnetic resonance spectra (1H NMR and 13C NMR spectra) were recorded on a Joel 400 MHz spectrometer with chemical shift values reported in δ units (ppm) relative to an internal standard. Elemental analyses were performed on Perkin-Elmer 2400 elemental analyzer, and the values found were within ±0.3% of the theoretical values. Followup of the reactions and checks of the purity of the compounds was done by TLC on silica gel-protected aluminum sheets (Type 60 GF254, Merck) and the spots were detected by exposure to UV-lamp at λ 254 nm for few seconds. The compounds were named using Chem. Draw Ultra version 11, Cambridge soft Corporation.


## 4.2. General Method for the Reaction ofN-Acetylisatin with Aminobenzoic Ester Derivatives

To the solution ofN-acetylisatin 1 (1.89 g, 10 mmol) in methanol (50 mL), which was heated up to 40–50°C, aminobenzoic ester (1.65 g, 10 mmol) was added with intensive stirring. The reaction mixture was refluxed with stirring for 2 h and then cooled down to room temperature. On the next day, the crystalline compound was collected with suction filtration, washed with little cold methanol, and dried under vacuum to afford the pure product.


### 4.2.1. Ethyl 4-(2-(2-Acetamidophenyl)-2-oxoacetamido)benzoate (2)

The product was obtained as a yellowish white solid from ethanol (mp 78–80°C) in 87% yield. IR (KBr): 3424.75, 3222.27 (NH), 1746.09 (CO-ester), 1694.36 (α-CO), 1654.00, 1603.36 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.26 (t, 3H, CH3CH2), 2.03 (s, 3H, COCH3), 4.20 (q, 2H, COOCH2CH3), 6.57 (d, 2H, Ar), 7.29 (t, 1H, Ar), 7.56 (d, 1H, Ar), 7.63–7.65 (m, 5H, NH, Ar), 10.44 (s, 1H, NHCOCH3) ppm. 13C NMR (d6-DMSO): δ = 14.92, 23.95, 53.38, 113.25, 116.68, 122.96, 124.80, 126.46, 130.89, 131.57, 134.53, 137.89, 153.86, 163.14, 166.42, 169.65, 185.61 ppm. C19H18N2O5 (354.36): Calcd. C 64.40, H 5.12, N 7.91; found: C 64.60, H 5.33, N 7.76.


### 4.2.2. Methyl 2-(2-Acetamidophenyl)-2-oxoacetate (7)

The product was obtained as yellow needles from methanol (mp 108–110°C) in 92% yield. IR (KBr): 3220.99 (NH), 1746.58 (CO-ester), 1696.22 (α-CO), 1656.79 (CONH) cm−1. 1H NMR (CDCl3): δ = 2.25 (s, 3H, COCH3), 3.99 (s, 3H, COOCH3), 7.14 (t, 1H, Ar), 7.64–7.68 (m, 2H, Ar), 8.78 (d, 1H, Ar), 11.06 (s, 1H, NH) ppm. 13C NMR (CDCl3): δ = 25.63, 53.08, 117.03, 120.76, 122.64, 133.63, 137.29, 142.77, 142.77, 163.95, 169.56, 190.34 ppm. C11H11NO4 (221.21): Calcd. C, 59.73; H, 5.01; N, 6.33; found: C 59.95, H 5.31, N 6.44.


## 4.2.1. Ethyl 4-(2-(2-Acetamidophenyl)-2-oxoacetamido)benzoate (2)

The product was obtained as a yellowish white solid from ethanol (mp 78–80°C) in 87% yield. IR (KBr): 3424.75, 3222.27 (NH), 1746.09 (CO-ester), 1694.36 (α-CO), 1654.00, 1603.36 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.26 (t, 3H, CH3CH2), 2.03 (s, 3H, COCH3), 4.20 (q, 2H, COOCH2CH3), 6.57 (d, 2H, Ar), 7.29 (t, 1H, Ar), 7.56 (d, 1H, Ar), 7.63–7.65 (m, 5H, NH, Ar), 10.44 (s, 1H, NHCOCH3) ppm. 13C NMR (d6-DMSO): δ = 14.92, 23.95, 53.38, 113.25, 116.68, 122.96, 124.80, 126.46, 130.89, 131.57, 134.53, 137.89, 153.86, 163.14, 166.42, 169.65, 185.61 ppm. C19H18N2O5 (354.36): Calcd. C 64.40, H 5.12, N 7.91; found: C 64.60, H 5.33, N 7.76.


## 4.2.2. Methyl 2-(2-Acetamidophenyl)-2-oxoacetate (7)

The product was obtained as yellow needles from methanol (mp 108–110°C) in 92% yield. IR (KBr): 3220.99 (NH), 1746.58 (CO-ester), 1696.22 (α-CO), 1656.79 (CONH) cm−1. 1H NMR (CDCl3): δ = 2.25 (s, 3H, COCH3), 3.99 (s, 3H, COOCH3), 7.14 (t, 1H, Ar), 7.64–7.68 (m, 2H, Ar), 8.78 (d, 1H, Ar), 11.06 (s, 1H, NH) ppm. 13C NMR (CDCl3): δ = 25.63, 53.08, 117.03, 120.76, 122.64, 133.63, 137.29, 142.77, 142.77, 163.95, 169.56, 190.34 ppm. C11H11NO4 (221.21): Calcd. C, 59.73; H, 5.01; N, 6.33; found: C 59.95, H 5.31, N 6.44.


## 4.3. General Method for the Synthesis of10a–d

To the solution ofN-acetylisatin 1 (1.89 g, 10 mmol) in acetonitrile (50 mL), amino acid ester hydrochloride [40, 41] (12 mmol) and K2CO3 (1.66 g, 12 mmol) were added with intensive stirring. The reaction mixture was stirred at room temperature overnight. On the next day, the reaction mixture was filtered with suction filtration and washed with 10 mL of acetonitrile. The solvent was removed under vacuum to dryness and the crude product was recrystallized from dichloromethane-hexane (1 : 3) to afford the pure product.


### 4.3.1. Ethyl 2-(2-(2-Acetamidophenyl)-2-oxoacetamido)acetate (10a)

The product was obtained as off-white solid (mp 108–110°C) in 89% yield. IR (KBr): broad peak at 3263.71 for two (NH), 1747.33 (CO-ester), 1673.41 (α-CO), 1645.10, 1579.62 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.23 (t, 3H, CH2CH3), 2.10 (s, 3H, COCH3), 4.00 (d, 2H, NHCH2CO), 4.15 (q, 2H, COOCH2CH3), 7.25 (t, 1H, Ar), 7.65 (t, 1H, Ar), 7.76 (d, 1H, Ar), 7.97 (d, 1H, Ar), 9.17 (s, 1H, NH), 10.65 (s, 1H, NH) ppm. 13C NMR (d6-DMSO): δ = 14.65, 24.73, 40.28, 61.31, 121.31, 123.38, 123.81, 132.58, 135.08, 139.50, 165.00, 169.49, 169.65, 191.95 ppm. C14H16N2O5 (292.11): Calcd. C 57.53, H 5.52, N 9.58;    found C 57.85, H 5.81, N 9.33.


### 4.3.2. Methyl 3-(2-(2-Acetamidophenyl)-2-oxoacetamido)propanoate (10b)

The product was obtained as off white solid (mp 90–92°C) in 87% yield. IR (KBr): 3287.56, 3124.00 (NH), 1740.76 (CO-ester), 1671.80 (α-CO), 1606.70, 1536.07 (CONH) cm−1. 1H NMR (CDCl3): δ = 2.18 (s, 3H, COCH3), 2.64 (t, 2H, CH2CH2CO), 3.67 (q, 2H, NHCH2), 3.70 (s, 3H, COOCH3), 7.09 (t, 1H, Ar), 7.45 (s, 1H, NH), 7.57 (t, 1H, Ar), 8.28 (d, 1H, Ar), 8.62 (d, 1H, Ar), 10.93 (s, 1H, NH). 13C NMR (CDCl3): δ = 25.52, 33.49, 35.06, 52.08, 118.57, 120.71, 122.58, 134.37, 136.62, 142.19, 163.05, 169.37, 172.61, 191.90 ppm. C14H16N2O5 (292.29): Calcd. C 57.53, H 5.52, N 9.58; found: C 57.28, H 5.81, N 9.33.


### 4.3.3. Methyl 4-(2-(2-Acetamidophenyl)-2-oxoacetamido)butanoate (10c)

The product was obtained as off white solid (mp 100–102°C) in 90% yield. IR (KBr): 3272.48, 3123.79 (NH), 1739.37 (CO-ester), 1667.80 (α-CO), 1606.09, 1532.29 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.75 (m, 2H, CH2CH2CH2), 2.05 (s, 3H, COCH3), 2.39 (t, 2H, CH2CH2CO), 3.21 (q, 2H, HNCH2CH2), 3.60 (s, 3H, COOCH3), 7.24 (t, 1H, Ar), 7.60–7.63 (m, 2H, Ar), 7.87 (d, 1H, Ar), 8.75 (t, 1H, CH2NH), 10.60 (1H, s, NHCO). 13C NMR (d6-DMSO): δ = 24.45, 26.68, 29.56, 31.13, 38.37, 51.87, 122.02, 124.08, 124.79, 131.97, 134.43, 138.76, 164.11, 169.39, 173.63, 191.69 ppm. C15H18N2O5 (306.31): Calcd. C 58.82, H 5.92, N 9.15; found: C 59.03, H 5.81, N 8.89.


### 4.3.4. Methyl 6-(2-(2-Acetamidophenyl)-2-oxoacetamido)hexanoate (10d)

The product was obtained as off white solid (mp 92–94°C) in 92% yield. IR (KBr): 3273.64, 3123.48 (NH), 1739.31 (CO-ester), 1667.77 (α-CO), 1606.05, 1532.23 (CONH) cm−1. 1H NMR (CDCl3): δ = 1.37–1.41 (m, 2H, CH2), 1.59–1.67 (m, 4H, 2CH2) 2.17 (s, 3H, COCH3), 2.31 (t, 2H, CH2), 3.37 (q, 2H, CH2), 3.64 (s, 3H, COOCH3), 7.03 (t, 1H, NHCH2), 7.09 (t, 1H, Ar), 7.54 (t, 1H, Ar), 8.26 (d, 1H, Ar), 8.57 (d, 1H, Ar), 10.93 (s, 1H, NHCO). 13C NMR (CDCl3): δ = 24.47, 25.50, 26.37, 29.00, 33.83, 39.39, 51.62, 118.76, 120.68, 122.64, 134.40, 136.51, 142.04, 163.17, 169.37, 174.01, 192.39 ppm. C17H22N2O5 (334.37): Calcd. C 61.07, H 6.63, N 8.38; found: C 61.23, H 6.79, N 8.57.


## 4.3.1. Ethyl 2-(2-(2-Acetamidophenyl)-2-oxoacetamido)acetate (10a)

The product was obtained as off-white solid (mp 108–110°C) in 89% yield. IR (KBr): broad peak at 3263.71 for two (NH), 1747.33 (CO-ester), 1673.41 (α-CO), 1645.10, 1579.62 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.23 (t, 3H, CH2CH3), 2.10 (s, 3H, COCH3), 4.00 (d, 2H, NHCH2CO), 4.15 (q, 2H, COOCH2CH3), 7.25 (t, 1H, Ar), 7.65 (t, 1H, Ar), 7.76 (d, 1H, Ar), 7.97 (d, 1H, Ar), 9.17 (s, 1H, NH), 10.65 (s, 1H, NH) ppm. 13C NMR (d6-DMSO): δ = 14.65, 24.73, 40.28, 61.31, 121.31, 123.38, 123.81, 132.58, 135.08, 139.50, 165.00, 169.49, 169.65, 191.95 ppm. C14H16N2O5 (292.11): Calcd. C 57.53, H 5.52, N 9.58;    found C 57.85, H 5.81, N 9.33.


## 4.3.2. Methyl 3-(2-(2-Acetamidophenyl)-2-oxoacetamido)propanoate (10b)

The product was obtained as off white solid (mp 90–92°C) in 87% yield. IR (KBr): 3287.56, 3124.00 (NH), 1740.76 (CO-ester), 1671.80 (α-CO), 1606.70, 1536.07 (CONH) cm−1. 1H NMR (CDCl3): δ = 2.18 (s, 3H, COCH3), 2.64 (t, 2H, CH2CH2CO), 3.67 (q, 2H, NHCH2), 3.70 (s, 3H, COOCH3), 7.09 (t, 1H, Ar), 7.45 (s, 1H, NH), 7.57 (t, 1H, Ar), 8.28 (d, 1H, Ar), 8.62 (d, 1H, Ar), 10.93 (s, 1H, NH). 13C NMR (CDCl3): δ = 25.52, 33.49, 35.06, 52.08, 118.57, 120.71, 122.58, 134.37, 136.62, 142.19, 163.05, 169.37, 172.61, 191.90 ppm. C14H16N2O5 (292.29): Calcd. C 57.53, H 5.52, N 9.58; found: C 57.28, H 5.81, N 9.33.


## 4.3.3. Methyl 4-(2-(2-Acetamidophenyl)-2-oxoacetamido)butanoate (10c)

The product was obtained as off white solid (mp 100–102°C) in 90% yield. IR (KBr): 3272.48, 3123.79 (NH), 1739.37 (CO-ester), 1667.80 (α-CO), 1606.09, 1532.29 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.75 (m, 2H, CH2CH2CH2), 2.05 (s, 3H, COCH3), 2.39 (t, 2H, CH2CH2CO), 3.21 (q, 2H, HNCH2CH2), 3.60 (s, 3H, COOCH3), 7.24 (t, 1H, Ar), 7.60–7.63 (m, 2H, Ar), 7.87 (d, 1H, Ar), 8.75 (t, 1H, CH2NH), 10.60 (1H, s, NHCO). 13C NMR (d6-DMSO): δ = 24.45, 26.68, 29.56, 31.13, 38.37, 51.87, 122.02, 124.08, 124.79, 131.97, 134.43, 138.76, 164.11, 169.39, 173.63, 191.69 ppm. C15H18N2O5 (306.31): Calcd. C 58.82, H 5.92, N 9.15; found: C 59.03, H 5.81, N 8.89.


## 4.3.4. Methyl 6-(2-(2-Acetamidophenyl)-2-oxoacetamido)hexanoate (10d)

The product was obtained as off white solid (mp 92–94°C) in 92% yield. IR (KBr): 3273.64, 3123.48 (NH), 1739.31 (CO-ester), 1667.77 (α-CO), 1606.05, 1532.23 (CONH) cm−1. 1H NMR (CDCl3): δ = 1.37–1.41 (m, 2H, CH2), 1.59–1.67 (m, 4H, 2CH2) 2.17 (s, 3H, COCH3), 2.31 (t, 2H, CH2), 3.37 (q, 2H, CH2), 3.64 (s, 3H, COOCH3), 7.03 (t, 1H, NHCH2), 7.09 (t, 1H, Ar), 7.54 (t, 1H, Ar), 8.26 (d, 1H, Ar), 8.57 (d, 1H, Ar), 10.93 (s, 1H, NHCO). 13C NMR (CDCl3): δ = 24.47, 25.50, 26.37, 29.00, 33.83, 39.39, 51.62, 118.76, 120.68, 122.64, 134.40, 136.51, 142.04, 163.17, 169.37, 174.01, 192.39 ppm. C17H22N2O5 (334.37): Calcd. C 61.07, H 6.63, N 8.38; found: C 61.23, H 6.79, N 8.57.


## 4.4. General Method for the Synthesis of (4 and 11a–d)

To the solution of glyoxyl derivatives (3 mmol) in methanol (15 mL), 0.7 mL of hydrazine hydrate (80%) was added. The reaction mixture was stirred at room temperature overnight. On the next day, the white precipitate was filtered with suction filtration and washed with cold 5 mL methanol (in case of there is no precipitation formed, the solvent was removed under vacuum and the crude product was washed with ether under stirring) to afford the product in pure state as observed from spectroscopic data.


### 4.4.1.  2-(2-Acetamidophenyl)-N-(4-(hydrazinecarbonyl)phenyl)acetamide (4)

The product was obtained as a white solid (mp 186–188°C) in 82% yield. IR (KBr): 3420.52, 3338.27, 3340.34, 3209.27 (NH2 and NH amide), 1676.44, 1611.89 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.20 (s, 3H, COCH3), 4.33 (brs, 2H, 2NH), 4.48 (s, 2H, C6H4CH2CO), 6.97–6.99 (m, 4H, Ar), 7.08 (s, 1H, NH), 7.17–7.23 (m, 4H, Ar), 9.39 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.38, 87.45, 123.52, 124.08, 125.56, 126.57, 129.45, 142.01, 157.46, 168.93 ppm. C17H18N4O3 (326.35): Calcd. C 62.57, H 5.56, N 17.17; found C 62.80, H 5.74, N 17.43.


### 4.4.2.  2-(2-Acetamidophenyl)-N-(2-hydrazinyl-2-oxoethyl)acetamide (11a)

The product was obtained as a white solid (mp 198–200°C) in 80% yield. IR (KBr): broad peak at 3275.61 (NH2 and NH amide), 1660.66, 1617.14, 1559.58 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.20 (s, 3H, COCH3), 3.69–3.79 (m, 2H, CH2), 4.24 (brs, 2H, 2NH), 4.71 (s, 2H, C6H4CH2CO), 6.98 (d, 1H, Ar), 7.16–7.24 (m, 3H, Ar), 8.48 (t, 1H, NH), 9.05 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.33, 41.59, 87.22, 123.68, 124.11, 125.49, 126.63, 129.43, 141.95, 157.40, 168.73, 170.72 ppm. C12H16N4O3 (264.28): Calcd. C 54.54, H 6.10, N 21.20; found: C 54.88, H 6.43, N 21.50.


### 4.4.3.  2-(2-Acetamidophenyl)-N-(3-hydrazinyl-3-oxopropyl)acetamide (11b)

The product was obtained as a white solid (mp 204–206°C) in 86% yield. IR (KBr): 3432.09, 3366.21, 3320.90, 3222.65 (NH2 and NH amide), 1666.91, 1593.01, 1559.48 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.17 (s, 3H, COCH3), 2.30 (t, 2H, CH2CH2CO), 3.36 (q, 2H, NHCH2CH2), 4.19 (brs, 2H, NH), 4.51 (s, 2H, C6H4CH2CO), 7.00 (m, 2H, Ar), 7.14 (d, 1H, Ar), 7.24 (t, 1H, Ar), 7.28 (s, 1H, NH), 8.33 (t, 1H, NH), 9.08 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.18, 33.58, 36.46, 87.35, 123.75, 123.97, 125.54, 126.31, 129.50, 141.78, 157.73, 169.85, 170.63 ppm. C13H18N4O3 (278.31): Calcd. C 56.10, H 6.52, N 20.13; found: C 55.87, H 6.81, N 19.89.


### 4.4.4.  2-(2-Acetamidophenyl)-N-(4-hydrazinyl-4-oxobutyl)acetamide (11c)

The product was obtained as a white solid (mp 195–197°C) in 84% yield. IR (KBr): 3344.24, 3304.18, 3260.00, 3219.07 (NH2 and NH amide), 1662.11, 1588.61, 1563.41 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.69 (m, 2H, CH2CH2CH2), 2.04 (t, 2H, CH2CO), 2.18 (s, 3H, COCH3), 3.14 (q, 2H, CH2CH2NH), 4.16 (brs, 2H, NH), 4.51 (s, 2H, C6H4CH2CO), 6.97–7.01 (m, 2H, Ar), 7.15–7.24 (m, 3H, 1NH, Ar), 8.34 (t, 1H, NH), 8.97 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.27, 25.83, 31.71, 40.32, 87.44, 123.62, 124.07, 126.27, 129.44, 157.63, 169.96, 171.95 ppm. C14H20N4O3 (292.33): Calcd. C 57.52, H 6.90, N 19.17; found: C 57.33, H 7.11, N 19.42.


### 4.4.5.  2-(2-Acetamidophenyl)-N-(6-hydrazinyl-6-oxohexyl)acetamide (11d)

The product was obtained as a white solid (mp 186–188°C) in 90% yield. IR (KBr): 3400.00, 3311.34, 3213.91 (br) (NH2 and NH amide), 1663.64, 1563.97, 1527.95 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.20–1.23 (m, 2H, CH2CH2CH2), 1.45–1.48 (m, 4H, CH2CH2CH2CH2), 2.00 (t, 2H, CH2CO), 2.17 (s, 3H, COCH3), 3.13 (q, 2H, CH2CH2NH), 4.15–4.45 (br, 2H, NH), 4.50 (s, 2H, C6H4CH2CO), 6.98–7.02 (m, 2H, Ar), 7.13–7.33 (m, 3H, NH, Ar), 8.30 (t, 1H, HNCH2), 8.96 (s, 1H, HNCOCH3). 13C NMR (d6-DMSO): δ = 22.16, 25.50, 26.59, 29.23, 33.92, 40.13, 87.43, 123.68, 123.97, 125.69, 126.26, 129.47, 141.81, 157.81, 169.80, 172.27 ppm. C16H24N4O3 (320.39): Calcd. C 59.98, H 7.55, N 17.49; found C 60.11, H 7.67, N 17.68.


## 4.4.1.  2-(2-Acetamidophenyl)-N-(4-(hydrazinecarbonyl)phenyl)acetamide (4)

The product was obtained as a white solid (mp 186–188°C) in 82% yield. IR (KBr): 3420.52, 3338.27, 3340.34, 3209.27 (NH2 and NH amide), 1676.44, 1611.89 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.20 (s, 3H, COCH3), 4.33 (brs, 2H, 2NH), 4.48 (s, 2H, C6H4CH2CO), 6.97–6.99 (m, 4H, Ar), 7.08 (s, 1H, NH), 7.17–7.23 (m, 4H, Ar), 9.39 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.38, 87.45, 123.52, 124.08, 125.56, 126.57, 129.45, 142.01, 157.46, 168.93 ppm. C17H18N4O3 (326.35): Calcd. C 62.57, H 5.56, N 17.17; found C 62.80, H 5.74, N 17.43.


## 4.4.2.  2-(2-Acetamidophenyl)-N-(2-hydrazinyl-2-oxoethyl)acetamide (11a)

The product was obtained as a white solid (mp 198–200°C) in 80% yield. IR (KBr): broad peak at 3275.61 (NH2 and NH amide), 1660.66, 1617.14, 1559.58 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.20 (s, 3H, COCH3), 3.69–3.79 (m, 2H, CH2), 4.24 (brs, 2H, 2NH), 4.71 (s, 2H, C6H4CH2CO), 6.98 (d, 1H, Ar), 7.16–7.24 (m, 3H, Ar), 8.48 (t, 1H, NH), 9.05 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.33, 41.59, 87.22, 123.68, 124.11, 125.49, 126.63, 129.43, 141.95, 157.40, 168.73, 170.72 ppm. C12H16N4O3 (264.28): Calcd. C 54.54, H 6.10, N 21.20; found: C 54.88, H 6.43, N 21.50.


## 4.4.3.  2-(2-Acetamidophenyl)-N-(3-hydrazinyl-3-oxopropyl)acetamide (11b)

The product was obtained as a white solid (mp 204–206°C) in 86% yield. IR (KBr): 3432.09, 3366.21, 3320.90, 3222.65 (NH2 and NH amide), 1666.91, 1593.01, 1559.48 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 2.17 (s, 3H, COCH3), 2.30 (t, 2H, CH2CH2CO), 3.36 (q, 2H, NHCH2CH2), 4.19 (brs, 2H, NH), 4.51 (s, 2H, C6H4CH2CO), 7.00 (m, 2H, Ar), 7.14 (d, 1H, Ar), 7.24 (t, 1H, Ar), 7.28 (s, 1H, NH), 8.33 (t, 1H, NH), 9.08 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.18, 33.58, 36.46, 87.35, 123.75, 123.97, 125.54, 126.31, 129.50, 141.78, 157.73, 169.85, 170.63 ppm. C13H18N4O3 (278.31): Calcd. C 56.10, H 6.52, N 20.13; found: C 55.87, H 6.81, N 19.89.


## 4.4.4.  2-(2-Acetamidophenyl)-N-(4-hydrazinyl-4-oxobutyl)acetamide (11c)

The product was obtained as a white solid (mp 195–197°C) in 84% yield. IR (KBr): 3344.24, 3304.18, 3260.00, 3219.07 (NH2 and NH amide), 1662.11, 1588.61, 1563.41 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.69 (m, 2H, CH2CH2CH2), 2.04 (t, 2H, CH2CO), 2.18 (s, 3H, COCH3), 3.14 (q, 2H, CH2CH2NH), 4.16 (brs, 2H, NH), 4.51 (s, 2H, C6H4CH2CO), 6.97–7.01 (m, 2H, Ar), 7.15–7.24 (m, 3H, 1NH, Ar), 8.34 (t, 1H, NH), 8.97 (s, 1H, NH). 13C NMR (d6-DMSO): δ = 22.27, 25.83, 31.71, 40.32, 87.44, 123.62, 124.07, 126.27, 129.44, 157.63, 169.96, 171.95 ppm. C14H20N4O3 (292.33): Calcd. C 57.52, H 6.90, N 19.17; found: C 57.33, H 7.11, N 19.42.


## 4.4.5.  2-(2-Acetamidophenyl)-N-(6-hydrazinyl-6-oxohexyl)acetamide (11d)

The product was obtained as a white solid (mp 186–188°C) in 90% yield. IR (KBr): 3400.00, 3311.34, 3213.91 (br) (NH2 and NH amide), 1663.64, 1563.97, 1527.95 (CONH) cm−1. 1H NMR (d6-DMSO): δ = 1.20–1.23 (m, 2H, CH2CH2CH2), 1.45–1.48 (m, 4H, CH2CH2CH2CH2), 2.00 (t, 2H, CH2CO), 2.17 (s, 3H, COCH3), 3.13 (q, 2H, CH2CH2NH), 4.15–4.45 (br, 2H, NH), 4.50 (s, 2H, C6H4CH2CO), 6.98–7.02 (m, 2H, Ar), 7.13–7.33 (m, 3H, NH, Ar), 8.30 (t, 1H, HNCH2), 8.96 (s, 1H, HNCOCH3). 13C NMR (d6-DMSO): δ = 22.16, 25.50, 26.59, 29.23, 33.92, 40.13, 87.43, 123.68, 123.97, 125.69, 126.26, 129.47, 141.81, 157.81, 169.80, 172.27 ppm. C16H24N4O3 (320.39): Calcd. C 59.98, H 7.55, N 17.49; found C 60.11, H 7.67, N 17.68.



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*Source: 901745-2013-11-21.xml*