# Weed Control in Dicamba-Resistant Soybean with Glyphosate/Dicamba Applied at Various Doses and Timings

Authors: Nader Soltani; Christy Shropshire; Peter H. Sikkema
Journal: International Journal of Agronomy
 (2020)

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## Abstract

Seven field trials were completed over a three-year period (2016 to 2018) in southwestern Ontario, Canada, to assess weed control in conventional-till dicamba-resistant (DR) soybean with glyphosate/dicamba (2 : 1 ratio) applied postemergence (POST) at 3 doses (900, 1350, and 1800 g·ae·ha−1) and 3 application timings (up to 5, 15, and 25 cm weeds). There was minimal soybean injury (≤2%) from treatments evaluated. Glyphosate/dicamba applied at application timing of up to 5, 15, and 25 cm weeds, controlled Amaranthus spp. (pigweed spp.) 87–96, 94–99, and 99%; Ambrosia artemisiifolia (common ragweed) 93–99, 97–99, and 99–100%; Chenopodium album (lambsquarters) 89–99, 95–100, and 99–100%; Echinochloa crus-galli (barnyardgrass) 81–84, 94–96, and 96–97%; Setaria faberi (giant foxtail) 37–90, 77–98, and 99–100%; and Setaria viridis (green foxtail) 94–96, 99, and 99–100%, respectively. Additionally, glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled Amaranthus spp. 90–97, 95–98, and 97–99%; A. artemisiifolia 95–98, 97–99, and 99–100%; C. album 92–99, 95–100, and 98–100%; E. crus-galli 84–88, 93-94, and 95-96%; S. faberi 74–95, 75–97, and 79–98%; and S. viridis 98, 98–99, and 98–100%, respectively. Weed interference reduced DR soybean yield as much as 51% compared to the highest yielding treatments. Results indicate that glyphosate/dicamba applied POST at the label doses can provide an adequate control of troublesome weeds in DR soybean. Weed control was generally most consistent when glyphosate/dicamba was applied at the highest registered dose in Ontario (1800 g·ae·ha−1) and when weeds were up to 25 cm tall.


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## Body

## 1. Introduction

Multiple-herbicide-resistant weeds, especially glyphosate-resistant (GR) weed biotypes, have increased rapidly in respect to number of weed species and geographic areas affected over the past 12 years in Ontario. Presently, there are four weed species with confirmed resistance to glyphosate in Ontario, includingConyza canadensis (L.) Cronq. (Canada fleabane), Amaranthus tuberculatus L. (waterhemp), Ambrosia trifida L. (giant ragweed), and Ambrosia artemisiifolia L. (common ragweed) [1–5]. As herbicide-resistant weeds are becoming more prevalent in many parts of North America, new soybean cultivars have been developed with transgenes that provide resistance to both glyphosate and dicamba (Roundup Ready Xtend®). Dicamba-resistant (DR) soybean has transgenes that provide resistance to glyphosate through an insensitive enolpyruvyl shikimate 3-phosphate synthase (CP4 EPSPS) enzyme and to dicamba through metabolism by dicamba monooxygenase (DMO) [6].DR soybean will allow soybean growers to incorporate dicamba, a Group 4 benzoic acid herbicide with excellent activity on nearly 100 annual, biennial, and perennial broadleaved weeds, into their cropping system. Despite being in use for more than 50 years, there are only six weed species that are currently confirmed resistant to dicamba worldwide [6, 7]. Dicamba also provides effective control of GR weeds including Amaranthus palmeri L. (palmer amaranth Wats.), A. tuberculatus, A. artemisiifolia, A. trifida, and C. canadensis [1, 5, 8, 9].DR soybean was first introduced in Ontario in 2017, and currently, over 50% of soybean fields in eastern Canada are seeded to DR soybean cultivars. The current registered dose of glyphosate/dicamba (2 : 1 ratio premix) in DR soybean in Ontario ranges from 900 (600 + 300) to 1800 (1200 + 600) g·ae·ha−1 [10].Earlier studies have mainly focused on GR weed control with glyphosate/dicamba in DR soybean in Ontario [1, 3–5, 9]. There have been little published data on the effect of glyphosate/dicamba for the control of other troublesome weeds in DR soybean in Ontario. Common problematic weeds in soybean in Ontario include Amaranthus spp. (green and redroot pigweed), A. artemisiifolia, Chenopodium album L. (common lambsquarters), Setaria viridis L. (green foxtail), Setaria faberi Herrm. (giant foxtail), and Echinochloa crus-galli (L.). P. Beauv. (barnyardgrass). The efficacy of glyphosate/dicamba applied postemergence (POST) at various application doses and application timings to control these troublesome weeds in DR soybean is not clear under Ontario environmental conditions. Dicamba, when used in combination with glyphosate in DR soybean, can provide effective broad-spectrum control of these weeds [11]. More research is also required to establish the optimal glyphosate/dicamba application dose when weeds are at different heights.This research was established to assess control of common annual grass and broadleaved weeds in conventional-till DR soybean with glyphosate/dicamba (2 : 1 ratio premix) applied POST at three doses (900, 1350, and 1800 g·ae·ha−1) and three application timings (up to 5, 15, and 25 cm weeds) in Ontario.


## 2. Materials and Methods

There was a total of seven field experiments which included two experiments (one in 2017 and 2018) at Exeter, ON (43° 19′ 1.2108″ N, 81° 30′ 3.8736″ E), and five experiments (two in 2016, one in 2017, and two in 2018) at Ridgetown, ON (42° 26′ 41.46″ N, 81° 52′ 44.472″ W). The experiment was arranged as a factorial with four replications and established as a randomized complete block design. Factor one consisted of three glyphosate/dicamba doses (DOSE: 0, 900, 1350, and 1800 g·ae·ha−1) and the second factor was application timing based on weed height (TIMING: up to 5, 15, and 25 cm weeds).Plots were 10 and 8 m long at Exeter and Ridgetown, respectively, and included four rows (spaced 75 cm apart) of DR soybean (“DKB 14-41”/“DKB 06-61”/“DKB 12-57”) seeded 4 cm deep at a seeding rate of 400,000 ha−1. Density of weed species present at each evaluation for Ridgetown and Exeter, ON, sites is shown in Table 1. Herbicides were applied using a CO2-pressurized backpack sprayer delivering 200 L·ha−1 at a pressure of 207 kPa. Herbicides were applied when weeds were up to approximately 5, 15, and 25 cm in height. The boom for the sprayer was 1.5 m long equipped with four ULD 120-02 (Hypro, New Brighton, Minnesota) nozzles spaced 0.5 m apart producing a spray width of 2.0 m (ground level).Table 1
Density of weed species present at each evaluation for Ridgetown and Exeter, ON, sites.
Weed species
Timing
Weed density (#m−2)
R-2016a
R-2016b
R-2017
E-2017
R-2018a
R-2018b
E-2018
Amaranthus spp.
4 WAA
57
19
19
11
11
—
6
8 WAA
35
17
21
9
10
—
4
10 WAC
50
5
10
9
13
—
2
Ambrosia artemisiifolia
4 WAA
9
14
—
12
54
23
—
8 WAA
9
28
—
12
21
8
—
10 WAC
12
17
—
12
13
7
—
Chenopodium album
4 WAA
33
10
12
12
21
23
5
8 WAA
30
25
14
12
15
13
3
10 WAC
18
10
13
13
10
13
1
Echinochloa crus-galli
4 WAA
—
—
—
9
—
3
19
8 WAA
—
—
—
8
—
7
12
10 WAC
—
—
—
8
—
9
12
Setaria faberi
4 WAA
99
114
70
—
3
—
—
8 WAA
143
138
86
—
4
—
—
10 WAC
136
82
88
—
4
—
—
Setaria viridis
4 WAA
—
—
—
26
19
28
25
8 WAA
—
—
—
26
12
22
18
10 WAC
—
—
—
24
6
18
12
Note.
∗—: not present at location; E: Exeter; R: Ridgetown; WAC: weeks after final herbicide application; WAA: weeks after herbicide application.Visible injury to DR soybean was assessed on a scale of 0–100 (0 = no injury and 100 = complete death) at 1 and 4 weeks after herbicide application (WAA). Weed control was evaluated on a 0–100 scale (0 = no control and 100 = complete control) at 4 and 8 WAA and 10 weeks after the final herbicide treatment (WAC). DR soybean was harvested at maturity using a small-plot combine. DR soybean seed moisture and yield (13% adjustment) were then recorded.The GLIMMIX procedure in SAS [12] was used for data analysis. The experimental design was used to construct the model used; fixed effects were DOSE, TIMING, and the DOSE by TIMING interaction, and random effects were environment, environment by DOSE by TIMING interaction, replicate within environment, the DOSE, and TIMING by replicate within environment interactions. Fit statistics and residual plots were assessed to determine the best distribution and associated link function for each parameter, which was then used to analyze data on the model scale. The normal distribution (identity link) was used for soybean yield, and the same distribution with an arcsine square root transformation was used for percent visible soybean injury and percent visible weed control. The gamma distribution (log link) was used to analyze soybean moisture at harvest. Tukey’s adjustment was applied to pairwise comparisons before determining treatment variations at P<0.05. The inverse link function or a back-transformation, where necessary, was applied to LSMEANS for presentation on the data scale. The DOSE by TIMING interaction was negligible for all parameters; therefore, only differences for the DOSE and TIMING main effects were determined [13]. The nontreated control, assigned a value of 0 for injury to the crop and weed control assessments, was excluded from the analysis because of no variance; differences between treatment means and the value zero were identified using the LSMEANS output.


## 3. Results and Discussion

### 3.1. Crop Response

Injury symptoms in soybean included leaf drooping and chlorosis. At 1 WAA, there was minimal visible soybean injury (<2%) from the herbicide treatments evaluated (Table2). Injury was slightly greater when glyphosate/dicamba was applied at late timing (up to 25 cm tall weeds) compared to the earlier timings (up to 5 or 15 cm tall weeds). According to Kniss [13] meta-analysis investigating soybean response to dicamba exposure, the likelihood for non-DT soybean injury is higher when the crop is exposed to dicamba at late timing compared to earlier timing. Glyphosate/dicamba applied POST at 1350 and 1800 g·ae·ha−1 caused slightly higher DR soybean injury than that at 900 g·ae·ha−1.Table 2
Significance of main effects and interaction for percent visible soybean injury 1 WAA, soybean moisture content at harvest, and soybean yield treated with glyphosate/dicamba at various POST application rates and timings at five Ridgetown, ON, sites (2016–2018) and two Exeter, ON, sites (2017-2018).
Main effects
Injury† (%)
Moisture (%)
Yield (T·ha−1)
Application timing
∗
∗
∗
∗
∗
5 cm weed height
0.2a
15.2b
2.8b
15 cm weed height
0.5a
14.9ab
3.1ab
25 cm weed height
1.6b
14.6a
3.2a
Glyphosate/dicamba rate
∗
∗
∗
∗
∗
∗
Untreated control
0a
16.1b
1.7b
900 g·ae·ha−1
0.1b
14.6a
3.4a
1350 g·ae·ha−1
0.7c
14.6a
3.5a
1800 g·ae·ha−1
1.3c
14.5a
3.5a
Interaction
R × T
NS
NS
NS
Note. NS: not significant at P=0.05 level; POST: postemergence; R: herbicide rate; T: application timing; WAA: weeks after herbicide application. Means followed by the same letter within a column are not significantly different according to a Tukey–Kramer multiple range test at P<0.05. Differences among simple effects are not presented due to negligible R × T interactions. Significance at P<0.05 and P<0.01 levels denoted by ∗ and ∗∗, respectively. †No soybean injury was observed at the 4 WAA evaluation.Soybean seed moisture content was less when glyphosate/dicamba was applied to up to 25 cm tall weeds compared to when it was applied to up to 5 cm tall weeds (Table2). There was no difference between other application timings. Glyphosate/dicamba applied POST at 900, 1350, or 1800 g·ae·ha−1 advanced DR soybean maturity as indicated by seed moisture content at harvest. There was no interaction between herbicide dose and application timing for injury, seed moisture content, and yield. The authors suggest that the delayed DR soybean maturity in the untreated control and with the early application of glyphosate/dicamba was due to greater weed interference. Soybean yield of untreated plots was as much as 51% lower than of treated plots, probably due to weed interference (Table 2). Results are similar to Underwood [14] who found as much as 41% yield loss from weed interference in DR soybean.


### 3.2.Amaranthus spp. (Green and Redroot Pigweed Combined)

The control ofAmaranthus spp. was generally lower with the early timing compared to the late timing of glyphosate/dicamba when weeds were up to 25 cm tall. Glyphosate/dicamba applied when weeds were approximately 5, 15, and 25 cm tall controlled Amaranthus spp. 96, 99, and 99% at 4 WAA; 92, 96, and 99% at 8 WAA; and 87, 94, and 99% at 10 WAC, respectively (Table 3). Glyphosate/dicamba at 900, 1350, and 1800 g·ae·ha−1 controlled Amaranthus spp. 97, 98, and 99% at 4 WAA; 94, 97, and 99% at 8 WAA; and 90, 95, and 97% at 10 WAC, respectively (Table 3). Results are similar to Johnson et al. [15] who found near-perfect control of Amaranthus spp. with glyphosate plus dicamba in DR soybean. Underwood [14] also reported 96–98% control of Amaranthus spp. at 2 WAA with glyphosate plus dicamba (900 + 300 g·ae·ha−1) in DR soybean.Table 3
Significance of main effects and interaction for percent visible control ofAmaranthus spp., Ambrosia artemisiifolia, and Chenopodium album in soybean treated with glyphosate/dicamba at various POST timings at four Ridgetown, ON, sites (2016–2018) and two Exeter, ON, sites (2017-2018).
Main effects
Amaranthus spp. control (%)
Ambrosia artemisiifoliacontrol (%)
Chenopodium album control (%)
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
Application timing
∗
∗
∗
∗
∗
∗
NS
∗
∗
∗
∗
∗
∗
5 cm weed height
96b
92b
87b
99a
95b
93b
99b
94b
89b
15 cm weed height
99a
96b
94b
99a
98ab
97ab
100a
96b
95ab
25 cm weed height
99a
99a
99a
99a
100a
99a
100a
99a
99a
Dose
∗
∗
∗
∗
NS
NS
∗
NS
NS
Untreated control
0c
0c
0c
0c
0b
0b
0 c
0b
0b
900 g·ae·ha−1
97b
94b
90b
98b
96a
95a
99b
94a
92a
1350 g·ae·ha−1
98ab
97ab
95ab
99ab
98a
97a
100a
97a
95a
1800 g·ae·ha−1
99a
99a
97a
100a
99a
99a
100a
99a
98a
Interaction
R × T
NS
NS
NS
NS
NS
NS
NS
NS
NS
Note. NS: not significant at P=0.05 level; POST: postemergence; R: herbicide rate; T: application timing; WAC: weeks after final herbicide application; WAA: weeks after herbicide application. Means followed by the same letter within a column are not significantly different according to a Tukey–Kramer multiple range test at P<0.05. Differences among simple effects are not presented due to negligible R × T interactions. Significance at P<0.05 and P<0.01 levels denoted by ∗ and ∗∗, respectively.


### 3.3.A. artemisiifolia

The control ofA. artemisiifolia tended to be generally lower with the early timing compared to the late timing of glyphosate/dicamba when weeds were up to 25 cm tall (Table 3). Glyphosate/dicamba applied at 5, 15, and 25 cm tall weeds controlled A. artemisiifolia 99, 99, and 99% at 4 WAA; 95, 98, and 100% at 8 WAA; and 93, 97, and 99% at 10 WAC, respectively (Table 3). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled A. artemisiifolia 98, 99, and 100% at 4 WAA; 96, 98, and 99% at 8 WAA; and 95, 97, and 99% at 10 WAC, respectively (Table 3). Results from this study are similar to Breitenbach et al. [16] who reported up to 99% control of A. artemisiifolia with glyphosate/dicamba in DR soybean in Minnesota, USA. Additionally, Underwood [14] reported that glyphosate plus dicamba (900 + 300 g·ae·ha−1) controlled A. artemisiifolia 98–100% in DR soybean.


### 3.4.C. album

The control ofC. album was generally lower with the early timing compared to the late timing of glyphosate/dicamba when weeds were up to 25 cm tall. Glyphosate/dicamba applied POST at up to 5, 15, and 25 cm tall weeds controlled C. album 99, 100, and 100% at 4 WAA; 94, 96, and 99% at 8 WAA; and 89, 95, and 99% at 10 WAC, respectively (Table 3). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled C. album 99, 100, and 100% at 4 WAA; 94, 97, and 99% at 8 WAA; and 92, 95, and 98% at 10 WAC, respectively (Table 3). Similar to this study, Breitenbach et al. [16] reported up to 99% control of C. album with glyphosate + dicamba in DR soybean in Rochester, MN.


### 3.5.E. crus-galli

The control ofE. crus-galli was significantly lower when glyphosate/dicamba was applied up to 5 cm tall weeds compared to 15 and 25 cm tall weeds (Table 4). Glyphosate/dicamba applied at application timing of up to 5, 15, and 25 cm tall weeds controlled E. crus-galli 84, 95, and 96% at 4 WAA; 81, 94, and 97% at 8 WAA; and 82, 96, and 96% at 10 WAC, respectively (Table 4). E. crus-galli control generally increased as the dose of the glyphosate/dicamba was increased. Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled E. crus-galli 84, 94, and 96% at 4 WAA; 87, 93, and 95% at 8 WAA; and 88, 93, and 95% at 10 WAC, respectively (Table 4). In other studies, Breitenbach et al. [16] reported 98, 97, 97, and 94% control of grass species with glyphosate/dicamba applied early POST in DR soybean in Rochester, MN. Additionally, Underwood [14] reported that glyphosate plus dicamba (900 + 300 g·ae·ha−1) controlled E. crus-galli as much as 99% in DR soybean.Table 4
Significance of main effects and interaction for percent visible control ofEchinochloa crus-galli, Setaria faberi, and Setaria viridis in soybean treated with glyphosate/dicamba at various POST timings at four Ridgetown, ON, sites (2016–2018) and two Exeter, ON, sites (2017-2018).
Main effects
Echinochloa crus-galli control (%)
Setaria faberi control (%)
Setaria viridis control (%)
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
Application timing
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
5 cm weed height
84b
81b
82b
90b
51b
37c
94b
96b
95b
15 cm weed height
95a
94a
96a
98a
80b
77b
99a
99a
99a
25 cm weed height
96a
97a
96a
100a
99a
99a
100a
100a
99a
Dose
∗
∗
NS
∗
∗
NS
NS
NS
∗
∗
∗
NS
Untreated control
0c
0b
0c
0b
0b
0b
0c
0c
0b
900 g·ae·ha−1
84b
87a
88b
95a
77a
74a
98b
98b
98a
1350 g·ae·ha−1
94a
93a
93ab
97a
80a
75a
99ab
99ab
98a
1800 g·ae·ha−1
96a
95a
95a
98a
87a
79a
100a
100a
98a
Interaction
R × T
NS
NS
NS
NS
NS
NS
NS
NS
NS
Note. NS: not significant at P=0.05 level; POST: postemergence; R: herbicide rate; T: application timing; WAC: weeks after final herbicide application; WAA: weeks after herbicide application. Means followed by the same letter within a column are not significantly different according to a Tukey–Kramer multiple range test at P<0.05. Differences among simple effects are not presented due to negligible R × T interactions. Significance at P<0.05 and P<0.01 levels denoted by ∗ and ∗∗, respectively.


### 3.6.S. faberi

The control ofS. faberi with glyphosate/dicamba tended to generally increase as the application timing was delayed (Table 4). Glyphosate/dicamba applied at up to 5, 15, and 25 cm tall weeds controlled S. faberi 90, 98, and 100% at 4 WAA; 51, 80, and 99% at 8 WAA; and 37, 77, and 99% at 10 WAC, respectively (Table 3). There was no appreciable influence of the application dose of glyphosate/dicamba on the control of S. faberi (Table 4). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled S. faberi 95, 97, and 98% at 4 WAA; 77, 80, and 87% at 8 WAA; and 74, 75, and 79% at 10 WAC, respectively (Table 4).


### 3.7.S. viridis

The control ofS. viridis with glyphosate/dicamba was higher when the timing was delayed (Table 4). Glyphosate/dicamba applied at application timing of up to 5, 15, and 25 cm tall weeds controlled S. viridis 94, 99, and 100% at 4 WAA; 96, 99, and 100% at 8 WAA; and 95, 99, and 99% at 10 WAC, respectively (Table 4). There was no appreciable influence of the application dose of glyphosate/dicamba on the control of S. viridis (Table 4). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled S. viridis 98, 99, and 100% at 4 WAA; 98, 99, and 100% at 8 WAA; and 98, 98, and 98% at 10 WAC, respectively (Table 4). Similar to this study, Underwood [14] reported that glyphosate plus dicamba (900 + 300 g·ae·ha−1) controlled S. viridis 93–97% in DR soybean.


## 3.1. Crop Response

Injury symptoms in soybean included leaf drooping and chlorosis. At 1 WAA, there was minimal visible soybean injury (<2%) from the herbicide treatments evaluated (Table2). Injury was slightly greater when glyphosate/dicamba was applied at late timing (up to 25 cm tall weeds) compared to the earlier timings (up to 5 or 15 cm tall weeds). According to Kniss [13] meta-analysis investigating soybean response to dicamba exposure, the likelihood for non-DT soybean injury is higher when the crop is exposed to dicamba at late timing compared to earlier timing. Glyphosate/dicamba applied POST at 1350 and 1800 g·ae·ha−1 caused slightly higher DR soybean injury than that at 900 g·ae·ha−1.Table 2
Significance of main effects and interaction for percent visible soybean injury 1 WAA, soybean moisture content at harvest, and soybean yield treated with glyphosate/dicamba at various POST application rates and timings at five Ridgetown, ON, sites (2016–2018) and two Exeter, ON, sites (2017-2018).
Main effects
Injury† (%)
Moisture (%)
Yield (T·ha−1)
Application timing
∗
∗
∗
∗
∗
5 cm weed height
0.2a
15.2b
2.8b
15 cm weed height
0.5a
14.9ab
3.1ab
25 cm weed height
1.6b
14.6a
3.2a
Glyphosate/dicamba rate
∗
∗
∗
∗
∗
∗
Untreated control
0a
16.1b
1.7b
900 g·ae·ha−1
0.1b
14.6a
3.4a
1350 g·ae·ha−1
0.7c
14.6a
3.5a
1800 g·ae·ha−1
1.3c
14.5a
3.5a
Interaction
R × T
NS
NS
NS
Note. NS: not significant at P=0.05 level; POST: postemergence; R: herbicide rate; T: application timing; WAA: weeks after herbicide application. Means followed by the same letter within a column are not significantly different according to a Tukey–Kramer multiple range test at P<0.05. Differences among simple effects are not presented due to negligible R × T interactions. Significance at P<0.05 and P<0.01 levels denoted by ∗ and ∗∗, respectively. †No soybean injury was observed at the 4 WAA evaluation.Soybean seed moisture content was less when glyphosate/dicamba was applied to up to 25 cm tall weeds compared to when it was applied to up to 5 cm tall weeds (Table2). There was no difference between other application timings. Glyphosate/dicamba applied POST at 900, 1350, or 1800 g·ae·ha−1 advanced DR soybean maturity as indicated by seed moisture content at harvest. There was no interaction between herbicide dose and application timing for injury, seed moisture content, and yield. The authors suggest that the delayed DR soybean maturity in the untreated control and with the early application of glyphosate/dicamba was due to greater weed interference. Soybean yield of untreated plots was as much as 51% lower than of treated plots, probably due to weed interference (Table 2). Results are similar to Underwood [14] who found as much as 41% yield loss from weed interference in DR soybean.


## 3.2.Amaranthus spp. (Green and Redroot Pigweed Combined)

The control ofAmaranthus spp. was generally lower with the early timing compared to the late timing of glyphosate/dicamba when weeds were up to 25 cm tall. Glyphosate/dicamba applied when weeds were approximately 5, 15, and 25 cm tall controlled Amaranthus spp. 96, 99, and 99% at 4 WAA; 92, 96, and 99% at 8 WAA; and 87, 94, and 99% at 10 WAC, respectively (Table 3). Glyphosate/dicamba at 900, 1350, and 1800 g·ae·ha−1 controlled Amaranthus spp. 97, 98, and 99% at 4 WAA; 94, 97, and 99% at 8 WAA; and 90, 95, and 97% at 10 WAC, respectively (Table 3). Results are similar to Johnson et al. [15] who found near-perfect control of Amaranthus spp. with glyphosate plus dicamba in DR soybean. Underwood [14] also reported 96–98% control of Amaranthus spp. at 2 WAA with glyphosate plus dicamba (900 + 300 g·ae·ha−1) in DR soybean.Table 3
Significance of main effects and interaction for percent visible control ofAmaranthus spp., Ambrosia artemisiifolia, and Chenopodium album in soybean treated with glyphosate/dicamba at various POST timings at four Ridgetown, ON, sites (2016–2018) and two Exeter, ON, sites (2017-2018).
Main effects
Amaranthus spp. control (%)
Ambrosia artemisiifoliacontrol (%)
Chenopodium album control (%)
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
Application timing
∗
∗
∗
∗
∗
∗
NS
∗
∗
∗
∗
∗
∗
5 cm weed height
96b
92b
87b
99a
95b
93b
99b
94b
89b
15 cm weed height
99a
96b
94b
99a
98ab
97ab
100a
96b
95ab
25 cm weed height
99a
99a
99a
99a
100a
99a
100a
99a
99a
Dose
∗
∗
∗
∗
NS
NS
∗
NS
NS
Untreated control
0c
0c
0c
0c
0b
0b
0 c
0b
0b
900 g·ae·ha−1
97b
94b
90b
98b
96a
95a
99b
94a
92a
1350 g·ae·ha−1
98ab
97ab
95ab
99ab
98a
97a
100a
97a
95a
1800 g·ae·ha−1
99a
99a
97a
100a
99a
99a
100a
99a
98a
Interaction
R × T
NS
NS
NS
NS
NS
NS
NS
NS
NS
Note. NS: not significant at P=0.05 level; POST: postemergence; R: herbicide rate; T: application timing; WAC: weeks after final herbicide application; WAA: weeks after herbicide application. Means followed by the same letter within a column are not significantly different according to a Tukey–Kramer multiple range test at P<0.05. Differences among simple effects are not presented due to negligible R × T interactions. Significance at P<0.05 and P<0.01 levels denoted by ∗ and ∗∗, respectively.


## 3.3.A. artemisiifolia

The control ofA. artemisiifolia tended to be generally lower with the early timing compared to the late timing of glyphosate/dicamba when weeds were up to 25 cm tall (Table 3). Glyphosate/dicamba applied at 5, 15, and 25 cm tall weeds controlled A. artemisiifolia 99, 99, and 99% at 4 WAA; 95, 98, and 100% at 8 WAA; and 93, 97, and 99% at 10 WAC, respectively (Table 3). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled A. artemisiifolia 98, 99, and 100% at 4 WAA; 96, 98, and 99% at 8 WAA; and 95, 97, and 99% at 10 WAC, respectively (Table 3). Results from this study are similar to Breitenbach et al. [16] who reported up to 99% control of A. artemisiifolia with glyphosate/dicamba in DR soybean in Minnesota, USA. Additionally, Underwood [14] reported that glyphosate plus dicamba (900 + 300 g·ae·ha−1) controlled A. artemisiifolia 98–100% in DR soybean.


## 3.4.C. album

The control ofC. album was generally lower with the early timing compared to the late timing of glyphosate/dicamba when weeds were up to 25 cm tall. Glyphosate/dicamba applied POST at up to 5, 15, and 25 cm tall weeds controlled C. album 99, 100, and 100% at 4 WAA; 94, 96, and 99% at 8 WAA; and 89, 95, and 99% at 10 WAC, respectively (Table 3). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled C. album 99, 100, and 100% at 4 WAA; 94, 97, and 99% at 8 WAA; and 92, 95, and 98% at 10 WAC, respectively (Table 3). Similar to this study, Breitenbach et al. [16] reported up to 99% control of C. album with glyphosate + dicamba in DR soybean in Rochester, MN.


## 3.5.E. crus-galli

The control ofE. crus-galli was significantly lower when glyphosate/dicamba was applied up to 5 cm tall weeds compared to 15 and 25 cm tall weeds (Table 4). Glyphosate/dicamba applied at application timing of up to 5, 15, and 25 cm tall weeds controlled E. crus-galli 84, 95, and 96% at 4 WAA; 81, 94, and 97% at 8 WAA; and 82, 96, and 96% at 10 WAC, respectively (Table 4). E. crus-galli control generally increased as the dose of the glyphosate/dicamba was increased. Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled E. crus-galli 84, 94, and 96% at 4 WAA; 87, 93, and 95% at 8 WAA; and 88, 93, and 95% at 10 WAC, respectively (Table 4). In other studies, Breitenbach et al. [16] reported 98, 97, 97, and 94% control of grass species with glyphosate/dicamba applied early POST in DR soybean in Rochester, MN. Additionally, Underwood [14] reported that glyphosate plus dicamba (900 + 300 g·ae·ha−1) controlled E. crus-galli as much as 99% in DR soybean.Table 4
Significance of main effects and interaction for percent visible control ofEchinochloa crus-galli, Setaria faberi, and Setaria viridis in soybean treated with glyphosate/dicamba at various POST timings at four Ridgetown, ON, sites (2016–2018) and two Exeter, ON, sites (2017-2018).
Main effects
Echinochloa crus-galli control (%)
Setaria faberi control (%)
Setaria viridis control (%)
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
4 WAA
8 WAA
10 WAC
Application timing
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
∗
5 cm weed height
84b
81b
82b
90b
51b
37c
94b
96b
95b
15 cm weed height
95a
94a
96a
98a
80b
77b
99a
99a
99a
25 cm weed height
96a
97a
96a
100a
99a
99a
100a
100a
99a
Dose
∗
∗
NS
∗
∗
NS
NS
NS
∗
∗
∗
NS
Untreated control
0c
0b
0c
0b
0b
0b
0c
0c
0b
900 g·ae·ha−1
84b
87a
88b
95a
77a
74a
98b
98b
98a
1350 g·ae·ha−1
94a
93a
93ab
97a
80a
75a
99ab
99ab
98a
1800 g·ae·ha−1
96a
95a
95a
98a
87a
79a
100a
100a
98a
Interaction
R × T
NS
NS
NS
NS
NS
NS
NS
NS
NS
Note. NS: not significant at P=0.05 level; POST: postemergence; R: herbicide rate; T: application timing; WAC: weeks after final herbicide application; WAA: weeks after herbicide application. Means followed by the same letter within a column are not significantly different according to a Tukey–Kramer multiple range test at P<0.05. Differences among simple effects are not presented due to negligible R × T interactions. Significance at P<0.05 and P<0.01 levels denoted by ∗ and ∗∗, respectively.


## 3.6.S. faberi

The control ofS. faberi with glyphosate/dicamba tended to generally increase as the application timing was delayed (Table 4). Glyphosate/dicamba applied at up to 5, 15, and 25 cm tall weeds controlled S. faberi 90, 98, and 100% at 4 WAA; 51, 80, and 99% at 8 WAA; and 37, 77, and 99% at 10 WAC, respectively (Table 3). There was no appreciable influence of the application dose of glyphosate/dicamba on the control of S. faberi (Table 4). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled S. faberi 95, 97, and 98% at 4 WAA; 77, 80, and 87% at 8 WAA; and 74, 75, and 79% at 10 WAC, respectively (Table 4).


## 3.7.S. viridis

The control ofS. viridis with glyphosate/dicamba was higher when the timing was delayed (Table 4). Glyphosate/dicamba applied at application timing of up to 5, 15, and 25 cm tall weeds controlled S. viridis 94, 99, and 100% at 4 WAA; 96, 99, and 100% at 8 WAA; and 95, 99, and 99% at 10 WAC, respectively (Table 4). There was no appreciable influence of the application dose of glyphosate/dicamba on the control of S. viridis (Table 4). Glyphosate/dicamba applied POST at 900, 1350, and 1800 g·ae·ha−1 controlled S. viridis 98, 99, and 100% at 4 WAA; 98, 99, and 100% at 8 WAA; and 98, 98, and 98% at 10 WAC, respectively (Table 4). Similar to this study, Underwood [14] reported that glyphosate plus dicamba (900 + 300 g·ae·ha−1) controlled S. viridis 93–97% in DR soybean.


## 4. Conclusions

Based on these results, glyphosate/dicamba applied postemergence at 900, 1350, and 1800 g·ae·ha−1 causes minimal injury in DR soybean. Presumed weed interference delayed DR soybean maturity and reduced DR soybean yield as much as 51%. There was an increase in DR soybean yield with the application of glyphosate/dicamba, but there was no difference in yield among application doses. DR soybean yield was lower with the early application of glyphosate/dicamba; the authors suggest that this was due to increased weed emergence and interference after the early application. There was a trend for increased weed control as the glyphosate/dicamba dose was increased and the application timing was delayed. Based on these results, glyphosate/dicamba at the manufacturer’s label doses adequately controls C. album, Amaranthus spp., A. artemisiifolia, S. faberi, S. viridis, and E. crus-galli in DR soybean. Weed control was generally least consistent with E. crus-galli and S. faberi especially when glyphosate/dicamba was applied at the lowest registered dose (900 g·ae·ha−1) and when these weeds were up to 5 cm tall. Weed control was generally most consistent when glyphosate/dicamba was applied at the highest registered dose in Ontario (1800 g·ae·ha−1) and when weeds were up to 25 cm tall. However, to reduce injury in neighbouring sensitive crops from the off-target movement of dicamba, it is recommended that glyphosate/dicamba be applied early in the season, preferably before sensitive crops emerge in adjacent fields. Long-term stewardship of the DR soybean technology is critical to minimize injury in sensitive crops due to off-target movement in adjacent fields and ensure that this valuable technology is available for the control of multiple-resistant weed biotypes.



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*Source: 8903941-2020-02-12.xml*