# Environmentally Smart Nitrogen Performance in Northern Great Plains’ Spring Wheat Production Systems

Authors: Olga S. Walsh; Kefyalew Girma
Journal: International Journal of Agronomy
 (2016)

---

## Abstract

Experiments were conducted in Montana to evaluate Environmentally Smart Nitrogen (ESN) as a nitrogen (N) source in wheat. Plots were arranged in a split-plot design with ESN, urea, and a 50%-50% urea-ESN blend at low, medium, and high at-seeding N rates in the subplot, with four replications. Measurements included grain yield (GY), protein (GP), and N uptake (GNU). A partial budget economic analysis was performed to assess the net benefits of the three sources. Average GY varied from 1816 to 5583 kg ha−1 and grain protein (GP) content ranged from 9.1 to 17.3% among site-years. Urea, ESN, and the blend resulted in higher GYs at 3, 2, and 2 site-years out of 8 evaluated site-years, respectively. Topdressing N improved GY for all sources. No trend in GP associated with N source was observed. With GP-adjusted revenue, farmer would not recover investment costs from ESN or blend compared with urea. With ESN costing consistently more than urea per unit of N, we recommend urea as N source for spring wheat in Northern Great Plains.


---

## Body

## 1. Introduction

To address the nutritional needs of the fast-growing human population, the annual cereal production will have to increase to about 3 billion tones by 2050 from currently produced 2.1 billion tones [1]. It was shown that 80 percent of the required crop production increases will have to come from improved GY and crop intensification versus only 20 percent from expansion of agricultural land [1, 2]. Furthermore, the concern is that the rate of growth in cereal GY has been steadily declining worldwide.The reports indicate that the rate of growth of major cereals has dropped from 3.2 percent per year in the 1960s to only 1.5 percent in 2000 [1, 2]. Although the genetics and biotech industries estimated their ability to increase cereal GYs due to genetic GY potential of 3 to 4% per year [3], history indicates that the genetic advances alone may not be sufficient to solve the world’s food shortage issues [4]. At least 50% of food currently produced in the world is possible due to use of commercial N, phosphorus (P), and potassium (K) fertilizer [5]. Roberts [4] pointed out the critical role the fertilizers play in the world’s food security and underlined the close correlation between global cereal crop production and fertilizer consumption. Consequently, the fertilizer use is estimated to increase dramatically worldwide at an expected rate of 2.5 million metric tons per year [6].Nitrogen use efficiency (NUE), the ratio of the difference of N uptake in the N treated plot and N uptake in the check and the total applied N rate, is only at 40 to 50% for most crop production systems [7]. A considerable improvement from the previously estimated 33% NUE in the late 1990s [8] is mainly due to advances in precision agriculture and novel fertilizer technologies [9]. Developing an effective and efficient N management strategy and improving N guidelines are the key challenges that must be addressed to maintain and enhance the sustainability of cereal crop production. Identifying practical solutions for reducing agricultural input costs such as fertilizer is critical for maintaining soil fertility and improving productivity for crop growers [10].The Northern Great Plains is the key region for production of top quality spring wheat grain. Spring wheat continues to be one of the major cereal crops for Montana and North Dakota, which account approximately for 20 and 44%, respectively, of all spring wheat produced in the United States [11]. Not considering water, inadequate N nutrition is considered to be the most GY-limiting factor in wheat production [12].The awareness and interest to enhanced-efficiency fertilizer technologies among the Northern Great Plains producers are apparent. This trend is mainly due to steady efforts to improve the efficiency of fertilizer use and to minimize the negative impact of intensive agricultural production on the environment. The key to both increasing NUE and reducing N losses is the synchronization of N availability with the crop requirements [10], which has been the basis of many enhanced-efficiency fertilizers [13]. Shaviv [14] has proposed that enhanced-efficiency fertilizers could be effectively used to improve synchrony between soil N availability and crop uptake requirements for N. The enhanced-efficiency fertilizer products can reduce nutrient losses to the environment and deliver improved availability of nutrients to the crop [15].Environmentally Smart Nitrogen (ESN), a common enhanced-efficiency fertilizer, is produced by coating urea granules with a polymer shell that allows for a slow-release of N to the soil. Literature review points to numerous studies showing that higher GYs and better crop quality could be achieved with ESN compared to conventional urea providing crop producers with a higher return on their fertilizer investment [16, 17]. Furthermore, due to the slow-release technology, the increase in NUE is achieved by reduction of N losses through denitrification, leaching, and volatilization. In the recent years, the interest in use of the enhanced-efficiency fertilizers has increased due to their potential to reduce negative environmental impact [18, 19] observed improved GY and significantly lower N losses with sidedress ESN application to corn.Recently, enhanced-efficiency fertilizers have been defined as “fertilizer products with characteristics that allow increased plant uptake and reduce the potential of nutrient losses to the environment (e.g., gaseous losses, leaching, or runoff) when compared to an appropriate reference product” by the Association of American Plant Food Control Officials [20]. Numerous reports showed that enhanced-efficiency fertilizer products can decrease the nitrous oxide (N2O) emission rate from soils, especially immediately after fertilizer application [18].The Environmental Protection Agency (EPA) estimated that, in 2012, N2O accounted for about 6% of all US greenhouse gas emissions associated with human activities; agricultural activities account for approximately 75% of all N2O emissions [21]. Halvorson et al. [22] observed a 42% reduction in N2O emissions compared to traditional urea (46-0-0). Hatfield and Parkin [23] suggested that the increase in GY could be due to prolonged period of greenness in crops, especially during the grain-filling period. Furthermore, the use of enhanced-efficiency fertilizers such as ESN resulted in improved NUE, demonstrating positive environmental and agronomic advantages. Because the ESN was formulated to minimize N loss, ESN application is considered an environmentally sound farming practice.In the USA the growers using ESN are eligible for payments through the Environmental Quality Incentives Program (EQIP), which helps to offset the higher cost of ESN compared to traditional uncoated urea. The ESN also may be beneficial for producers in complying with tighter environmental regulations, shorter crop seeding windows, and adverse weather conditions [24].Some consider the ESN as a controlled release N fertilizer [25, 26] that supplies the crop with N all throughout the growing season [13]. Moreover, the manufacturer affirms that ESN offers a predictable release of N due to the coating characteristics. The polymer coating represents a semipermeable membrane that allows water to diffuse into the granule and dissolve N; encapsulated N remains inside and is released over time at a controlled rate [25].The N release rate is controlled by soil temperature; N release rate increases as the soils temperatures rise in the spring. This methodology aims to match crop need for N by “spoon-feeding” it with gradual N release from the ESN capsules. However, others tend to classify ESN simply as a slow-release product [27, 28] due to uncertainty in the level of control provided by the polymer coating. In fact, as water diffuses through a flexible, microthin polymer coating, the liquefied N is allowed to disperse out through the membrane and into the soil [29].The common variations in soil characteristics and temperatures must be also considered. Soil and air temperatures, as well as crop development rate, do not increase linearly throughout the growing season. In most cropping systems, warmer/colder periods are likely; the speed of crop growth is known to vary considerably depending on the developmental growth stage. For instance, stem elongation (Zadoks 30–39) is the most rapid stage of wheat crop growth, resulting in much faster vegetative tissue growth and biomass production compared to other growth stages [30].Studies conducted in a variety of crops have indicated that weather conditions, soil characteristics, application time, method, and crop rotations are the major factors influencing the effectiveness of the enhanced-efficiency [31–34]. Studies have shown that as all N has been dissolved within the ESN casing and the soil temperatures reach 20°C, between 65 and 90% of N is released into the soil within a 30-day period [35]. Also, a comprehensive assessment of N release from the ESN granules revealed that the rate of N release is highly dependent on the soil type. A more rapid release of N was observed in clay soils compared with silt and sandy loam soils [35].To meet a producers’ GY and quality targets for specific crops and growing conditions, ESN can be used as a sole N source or as a blend with other fertilizers such as traditional urea. Typically, ESN blended with other soluble N products performs best as spring preplant applications in spring wheat. Research in potato also has shown that a one-time application of ESN can be as cost effective as multiple in-season applications of traditional N products with fertigation [36]. Better or similar potato GYs were observed with a single application of ESN (even when 25% less N was applied), compared to those obtained with the split urea application [37].The ESN recommendations for a wide variety of field crops such as corn, canola, cotton, and wheat have been developed by the manufacturer. The manufacturer’s guidelines for ESN use in spring wheat in the Great Plains area are application of N as 100% ESN in the fall prior to seeding. Alternatively, spring wheat can be fertilized with a blend (40–75% ESN + 25–60% urea) [25]. This paper summarizes the results from a three-year-long study that compared the effect of ESN and urea-ESN blend with urea, for spring wheat production in the Northern Great Plains. The specific objectives of the study were to evaluate urea, ESN, and ESN-urea blend along at-seeding and topdress N rates on spring wheat production, quality, and economic return in Montana.


## 2. Materials and Methods

### 2.1. Experimental Sites and Treatment Structure

Three field studies were initiated in the spring of 2011 and completed in the 2013 growing season (eight site-years). Field trials were conducted at one irrigated site, at Western Agricultural Research Center (Corvallis, 46°19′39.8532′′, −114°05′08.6496′′) and two dryland sites at the North Western Agricultural Research Center (Kalispell, 48°11′14.4312′′, −114°08′04.7436′′) and at the Western Triangle Agricultural Research Center (Conrad, 48°19′11.6868′′, −111°55′28.7256′′).The soils at Conrad, Corvallis, and Kalispell are Scobey clay loam (fine, smectitic, frigid Aridic Argiustolls), burnt fork loam (coarse-loamy, mixed, superactive, frigid Typic Haplustolls), and Creston silt loam (fine-silty, mixed, superactive Typic Haploborolls), respectively. Initial soil properties, growing conditions, and field activities are presented in Table1.Table 1
Initial soil chemical properties (0–30 cm), growing conditions, and summary of field activities at Conrad, Corvallis, and Kalispell, MT, in 2011–2013.
Field activity
2011
2012Conrad
2013
Herbicide
GoldSky
GoldSky, Huskie
GoldSky, Huskie
Herbicide date
May 31
May 16
May 23
Sensing date
June 15
May 18
May 13
Topdress N date
June 15
May 21
May 14
Harvest date
August 30
August 16
September 10
Soil test N, kg ha−1
22
56
51
Soil P, ppm (Olson)
18
12
32
Soil K, ppm
221
152
234
Organic matter, %
1.5
1.2Corvallis
1.8
Herbicide
Bronate, Axial XL
Bronate, Axial XL
Supremacy, Axial XL
Herbicide date
June 13
May 28
May 28
Sensing date
June 15
June 8
June 5
Topdress N date
June 15
June 8
June 5
Harvest date
September 8
August 16
August 19
Soil test N, kg N ha−1
37.5
44.3
51.6
Soil P, ppm
23
23
25
Soil K, ppm
423
423
398
Organic matter, %
2.9
2.9Kalispell
2.2
Herbicide
Bronate, Axial XL
Bronate, Axial XL
—
Herbicide date
June 13
May 28
—
Sensing date
June 15
June 8
—
Topdress N date
June 15
June 8
—
Harvest date
September 8
August 16
—
Soil test N, kg N ha−1
37.5
44.3
—
Soil P, ppm
23
23
—
Soil K, ppm
423
425
—
Organic matter, %
2.8
2.9
—Plots were arranged in a split-plot design with three N sources and at-seeding N rates in the main plot and topdress N in the subplot. An additional control (0 kg N ha−1) treatment was included outside the factorial in the main plot. Each treatment was replicated 4 times at each site-year. The three N sources were urea, ESN, and a 50 : 50 blend of urea and ESN. The at-seeding N fertilizer rates were 56, 112, and 168 kg N ha−1 at Conrad and 112, 224, and 336 kg N ha−1 at Corvallis and Kalispell, representing low, medium, and high N rates, respectively, for each site. The at-seeding N rates were established based on the respective area’s GY goal and current Montana State University’s N fertilization guidelines for spring wheat. The topdress N was applied at 0 or 45 kg N ha−1 as granular broadcasted urea at Zadoks 30 (late tillering/beginning of stem elongation) growth stage.Hard red spring wheat (cv. Choteau) was seeded into plots measuring 1.5 by 7.6 m at the seeding rate of 1,830,000 plants ha−1 for dryland sites and from 2,150,000 to 3,225,000 plants per ha−1 at the irrigated site, using the custom-made direct-seed drill with Conserva Pak™ openers manufactured by Swift Machining (Washougal, WA). At-seeding N fertilizer was sidebanded about 1.90 cm to the side and about 2.54 cm above the seed.


### 2.2. Measurements

At maturity, spring wheat was harvested with Hege 125 plot combine in 2011 and 2012 and Wintersteiger classic plot combine in 2013. The harvested grain was dried in the drying room for 14 days at the temperature of 35°C; then, the by-plot GY was determined. From each by-plot sample, 400 g subsample was drawn and analyzed by the Agvise Laboratories (Northwood, ND) for total grain N content using near infrared reflectance spectroscopy (NIR) with a Perten DA 7250 NIR analyzer (Perten Instruments, Inc., Springfield, IL).From the GY and lab analysis results, grain N uptake (GNU) was determined using the following relationship:(1)GNU=GY∗N  content  of  grain.Furthermore, GP of spring wheat was calculated by multiplying the N content of grain by 6.5.


### 2.3. Statistical Analysis

All data were subjected to analysis of variance (ANOVA) using GLIMMIX procedure in SAS (SAS version 9.4, Cary, NC, USA). Before testing hypotheses, assumptions of normality and homogeneity of variance were checked for all measured variables using the UNIVARIATE procedure and Levene’s Homogeneity of Variance Test, respectively. Very few outliers were identified and removed from the data.After preliminary statistical analysis showed a significant site and year effects, data were analyzed for each site-year. In this three-factor study, the focus was on the two-way interaction effects (plus selected three-way interactions) of sources with at-seeding and topdress N rate treatment levels rather than the main effects. Whenever interactions were significant, tests of simple effects were generated and further dissected using the “slice” option under the least square (LS) means statement [38] in GLIMMIX for each factor level, at fixed level of the other factor. The test produces F value by extracting the appropriate rows from the coefficient matrix for each two-way interaction simple effect LS means [38, 39]. Then the “lines” statement under LS means statement was used to generate letter based pairwise LS means differences at p
<
0.05 if not specified for each response variable.Additionally, orthogonal polynomial contrasts were used to assess trends (linear and quadratic) in response variables against increasing rates of at-seeding N rate main effects and their interaction effect with source or topdress N levels when significant at least atp
<
0.1. Pearson product-moment correlation [40] analysis was used to assess the relationship of GY and GP. Pearson’s correlation was performed for each site-year. If no significant interaction effects were observed, then main effects were discussed.


### 2.4. Partial Budget Economic Analysis

A partial budget economic analysis [41] was performed to assess the net benefits of the three sources. The marginal benefit and rate of return [42] of the ESN and blend relative to N from urea were also evaluated, using the optimum N rates for each significant site-year (168 kg N ha−1 at Conrad and 224 kg N ha−1 at Corvallis and Kalispell, Table 2). An additional partial budget analysis was performed to compare ESN at medium at-seeding N rate with urea at low at-seeding N rate along topdress N rate. For this analysis the medium at-seeding rates were 112 and 224 kg N ha−1 at Conrad and Kalispell, respectively. The low at-seeding rates were 56 and 112 kg N ha−1 at Conrad and Kalispell, respectively, along 45 kg ha−1 topdress N. The calculation assumed spring wheat grain GY price unadjusted and adjusted for GP premium and discount. Premium and discount values were obtained from West-Con Cooperative [43]. Price of wheat was set at $0.17 kg−1 [44]. Current price of N in urea and ESN was set at $1.31 and 1.71 kg−1, respectively [45]. The cost of mixing urea and ESN blend was estimated at $3 ha−1. The cost of topdress application of N was set to be $15.2 ha−1. Partial budget equations were defined as follows [42]:(2)NR=TR-TVC,ΔNR=ΔTR-ΔTVC,MRR=ΔTRΔTVC,where NR is net return, TR is total revenue, TVC is total variable cost, and ΔNR, ΔTR, and ΔTVC represent the change in net return, change in total revenue, and change in total variable cost due to ESN and ESN-urea blend, respectively, and RR is the rate of return that measures the dollar recovery to a dollar investment on ESN or blend.Table 2
Effect of N source and preplant and topdress N rate on spring wheat grain yield at Conrad, Corvallis, and Kalispell, MT, in 2011–2013 growing seasons.
Effect
Conrad
Corvallis
Kalispell
2011
2012
2013
2011
2012
2013
2011
2012
N source
kg ha−1
Urea
2488
5111b
£
4506
3161a
4640
2623
3699b
4506
ESN
2556
5515a
4439
2690b
4842
2488
3901a
b
4304
Blend
—
5447a
b
4708
2757b
4640
2152
4035a
4371
Prob >F
0.969
0.051
0.117
<0.001
0.699
0.452
0.003
0.517
At-seeding N rate, kg ha−1
0
2488
4035
3699
2623
3833
1816
2898
3833
Low†
2556
5178
4640
2757
4304
2556
4237
4371
Medium
2892
5447
4573
2959
5044
2556
4237
4371
High
3161
5582
4439
3026
4640
2152
4102
4371
Prob >F (trend)
0.006‡
0.003‡
0.055
0.048‡
0.050
0.051
0.114
0.945
Topdress N rate, kg ha−1
0
2389b
§
5380
4573
2690b
4506b
2354
3699b
4371
45
2741a
5447
4506
3026a
4977a
2488
4035a
4439
Prob >F
<0.001
0.749
0.410
<0.001
<0.001
0.813
0.003
0.175
£Down a column for each site-year source means followed by different letter are significant at p
<
0.05.
†At-seeding N rates: low, medium, and high were 56, 112, and 168 kg ha−1 for Conrad and 112, 224, and 336 kg ha−1 for Corvallis and Kalispell.
‡ represents significant linear trend, for at-seeding N rate for each site-year.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.


## 2.1. Experimental Sites and Treatment Structure

Three field studies were initiated in the spring of 2011 and completed in the 2013 growing season (eight site-years). Field trials were conducted at one irrigated site, at Western Agricultural Research Center (Corvallis, 46°19′39.8532′′, −114°05′08.6496′′) and two dryland sites at the North Western Agricultural Research Center (Kalispell, 48°11′14.4312′′, −114°08′04.7436′′) and at the Western Triangle Agricultural Research Center (Conrad, 48°19′11.6868′′, −111°55′28.7256′′).The soils at Conrad, Corvallis, and Kalispell are Scobey clay loam (fine, smectitic, frigid Aridic Argiustolls), burnt fork loam (coarse-loamy, mixed, superactive, frigid Typic Haplustolls), and Creston silt loam (fine-silty, mixed, superactive Typic Haploborolls), respectively. Initial soil properties, growing conditions, and field activities are presented in Table1.Table 1
Initial soil chemical properties (0–30 cm), growing conditions, and summary of field activities at Conrad, Corvallis, and Kalispell, MT, in 2011–2013.
Field activity
2011
2012Conrad
2013
Herbicide
GoldSky
GoldSky, Huskie
GoldSky, Huskie
Herbicide date
May 31
May 16
May 23
Sensing date
June 15
May 18
May 13
Topdress N date
June 15
May 21
May 14
Harvest date
August 30
August 16
September 10
Soil test N, kg ha−1
22
56
51
Soil P, ppm (Olson)
18
12
32
Soil K, ppm
221
152
234
Organic matter, %
1.5
1.2Corvallis
1.8
Herbicide
Bronate, Axial XL
Bronate, Axial XL
Supremacy, Axial XL
Herbicide date
June 13
May 28
May 28
Sensing date
June 15
June 8
June 5
Topdress N date
June 15
June 8
June 5
Harvest date
September 8
August 16
August 19
Soil test N, kg N ha−1
37.5
44.3
51.6
Soil P, ppm
23
23
25
Soil K, ppm
423
423
398
Organic matter, %
2.9
2.9Kalispell
2.2
Herbicide
Bronate, Axial XL
Bronate, Axial XL
—
Herbicide date
June 13
May 28
—
Sensing date
June 15
June 8
—
Topdress N date
June 15
June 8
—
Harvest date
September 8
August 16
—
Soil test N, kg N ha−1
37.5
44.3
—
Soil P, ppm
23
23
—
Soil K, ppm
423
425
—
Organic matter, %
2.8
2.9
—Plots were arranged in a split-plot design with three N sources and at-seeding N rates in the main plot and topdress N in the subplot. An additional control (0 kg N ha−1) treatment was included outside the factorial in the main plot. Each treatment was replicated 4 times at each site-year. The three N sources were urea, ESN, and a 50 : 50 blend of urea and ESN. The at-seeding N fertilizer rates were 56, 112, and 168 kg N ha−1 at Conrad and 112, 224, and 336 kg N ha−1 at Corvallis and Kalispell, representing low, medium, and high N rates, respectively, for each site. The at-seeding N rates were established based on the respective area’s GY goal and current Montana State University’s N fertilization guidelines for spring wheat. The topdress N was applied at 0 or 45 kg N ha−1 as granular broadcasted urea at Zadoks 30 (late tillering/beginning of stem elongation) growth stage.Hard red spring wheat (cv. Choteau) was seeded into plots measuring 1.5 by 7.6 m at the seeding rate of 1,830,000 plants ha−1 for dryland sites and from 2,150,000 to 3,225,000 plants per ha−1 at the irrigated site, using the custom-made direct-seed drill with Conserva Pak™ openers manufactured by Swift Machining (Washougal, WA). At-seeding N fertilizer was sidebanded about 1.90 cm to the side and about 2.54 cm above the seed.


## 2.2. Measurements

At maturity, spring wheat was harvested with Hege 125 plot combine in 2011 and 2012 and Wintersteiger classic plot combine in 2013. The harvested grain was dried in the drying room for 14 days at the temperature of 35°C; then, the by-plot GY was determined. From each by-plot sample, 400 g subsample was drawn and analyzed by the Agvise Laboratories (Northwood, ND) for total grain N content using near infrared reflectance spectroscopy (NIR) with a Perten DA 7250 NIR analyzer (Perten Instruments, Inc., Springfield, IL).From the GY and lab analysis results, grain N uptake (GNU) was determined using the following relationship:(1)GNU=GY∗N  content  of  grain.Furthermore, GP of spring wheat was calculated by multiplying the N content of grain by 6.5.


## 2.3. Statistical Analysis

All data were subjected to analysis of variance (ANOVA) using GLIMMIX procedure in SAS (SAS version 9.4, Cary, NC, USA). Before testing hypotheses, assumptions of normality and homogeneity of variance were checked for all measured variables using the UNIVARIATE procedure and Levene’s Homogeneity of Variance Test, respectively. Very few outliers were identified and removed from the data.After preliminary statistical analysis showed a significant site and year effects, data were analyzed for each site-year. In this three-factor study, the focus was on the two-way interaction effects (plus selected three-way interactions) of sources with at-seeding and topdress N rate treatment levels rather than the main effects. Whenever interactions were significant, tests of simple effects were generated and further dissected using the “slice” option under the least square (LS) means statement [38] in GLIMMIX for each factor level, at fixed level of the other factor. The test produces F value by extracting the appropriate rows from the coefficient matrix for each two-way interaction simple effect LS means [38, 39]. Then the “lines” statement under LS means statement was used to generate letter based pairwise LS means differences at p
<
0.05 if not specified for each response variable.Additionally, orthogonal polynomial contrasts were used to assess trends (linear and quadratic) in response variables against increasing rates of at-seeding N rate main effects and their interaction effect with source or topdress N levels when significant at least atp
<
0.1. Pearson product-moment correlation [40] analysis was used to assess the relationship of GY and GP. Pearson’s correlation was performed for each site-year. If no significant interaction effects were observed, then main effects were discussed.


## 2.4. Partial Budget Economic Analysis

A partial budget economic analysis [41] was performed to assess the net benefits of the three sources. The marginal benefit and rate of return [42] of the ESN and blend relative to N from urea were also evaluated, using the optimum N rates for each significant site-year (168 kg N ha−1 at Conrad and 224 kg N ha−1 at Corvallis and Kalispell, Table 2). An additional partial budget analysis was performed to compare ESN at medium at-seeding N rate with urea at low at-seeding N rate along topdress N rate. For this analysis the medium at-seeding rates were 112 and 224 kg N ha−1 at Conrad and Kalispell, respectively. The low at-seeding rates were 56 and 112 kg N ha−1 at Conrad and Kalispell, respectively, along 45 kg ha−1 topdress N. The calculation assumed spring wheat grain GY price unadjusted and adjusted for GP premium and discount. Premium and discount values were obtained from West-Con Cooperative [43]. Price of wheat was set at $0.17 kg−1 [44]. Current price of N in urea and ESN was set at $1.31 and 1.71 kg−1, respectively [45]. The cost of mixing urea and ESN blend was estimated at $3 ha−1. The cost of topdress application of N was set to be $15.2 ha−1. Partial budget equations were defined as follows [42]:(2)NR=TR-TVC,ΔNR=ΔTR-ΔTVC,MRR=ΔTRΔTVC,where NR is net return, TR is total revenue, TVC is total variable cost, and ΔNR, ΔTR, and ΔTVC represent the change in net return, change in total revenue, and change in total variable cost due to ESN and ESN-urea blend, respectively, and RR is the rate of return that measures the dollar recovery to a dollar investment on ESN or blend.Table 2
Effect of N source and preplant and topdress N rate on spring wheat grain yield at Conrad, Corvallis, and Kalispell, MT, in 2011–2013 growing seasons.
Effect
Conrad
Corvallis
Kalispell
2011
2012
2013
2011
2012
2013
2011
2012
N source
kg ha−1
Urea
2488
5111b
£
4506
3161a
4640
2623
3699b
4506
ESN
2556
5515a
4439
2690b
4842
2488
3901a
b
4304
Blend
—
5447a
b
4708
2757b
4640
2152
4035a
4371
Prob >F
0.969
0.051
0.117
<0.001
0.699
0.452
0.003
0.517
At-seeding N rate, kg ha−1
0
2488
4035
3699
2623
3833
1816
2898
3833
Low†
2556
5178
4640
2757
4304
2556
4237
4371
Medium
2892
5447
4573
2959
5044
2556
4237
4371
High
3161
5582
4439
3026
4640
2152
4102
4371
Prob >F (trend)
0.006‡
0.003‡
0.055
0.048‡
0.050
0.051
0.114
0.945
Topdress N rate, kg ha−1
0
2389b
§
5380
4573
2690b
4506b
2354
3699b
4371
45
2741a
5447
4506
3026a
4977a
2488
4035a
4439
Prob >F
<0.001
0.749
0.410
<0.001
<0.001
0.813
0.003
0.175
£Down a column for each site-year source means followed by different letter are significant at p
<
0.05.
†At-seeding N rates: low, medium, and high were 56, 112, and 168 kg ha−1 for Conrad and 112, 224, and 336 kg ha−1 for Corvallis and Kalispell.
‡ represents significant linear trend, for at-seeding N rate for each site-year.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.


## 3. Results

### 3.1. Grain Yield

In 2011, the lowest GY was observed at Conrad (2488 kg ha−1), followed by Corvallis (2623 to 3161 kg ha−1). Grain yield at Kalispell was the highest among the three experimental sites, ranging from 2898 to 4237 kg ha−1. In 2012, GY was the highest among the three growing seasons for all experimental locations. Conrad was the highest producing site with GY ranging from 4035 to 5583 kg ha−1. The GY for Kalispell and Corvallis was comparable but slightly higher at Corvallis. In 2013, GY was higher at Conrad (3699 to 4640 kg ha−1) than Corvallis (1816 to 2623 kg ha−1).Nitrogen source affected GY significantly at Conrad in 2012 and Corvallis and Kalispell in 2011 (Table2). At Conrad, ESN surpassed urea by 405 kg ha−1. At Corvallis, urea resulted in 16% more GY than ESN and blend combined. In 2011, at Kalispell, the blend yielded 336 kg ha−1 more compared to urea alone (Table 2). Overall, urea-ESN blend resulted in a significantly higher GY at Kalispell.At-seeding N rate significantly affected wheat GY at Conrad in 2011 and 2012 and at Corvallis in 2012 (Table2). Wheat GY increased linearly from 2488 to 3161 kg ha−1 in 2011 and from 4035 kg ha−1 to 5582 kg ha−1 in 2012 at Conrad when at-seeding N rate increased from 0 to 168 kg ha−1. Similarly, a linear increase from 2623 to 3026 kg ha−1 was observed in 2011 at Corvallis when at-seeding N rate increased from 0 to 336 kg ha−1 (Table 2). A quadratic trend was observed at Corvallis in 2012 and 2013 where GY reached peak at the medium N rate (224 kg ha−1).The topdress N treatment significantly affected GY in four of the eight site-years. At Conrad in 2011, Corvallis in 2011 and 2012, and Kalispell 2011, topdressing 45 kg N ha−1 resulted in 14.7, 12.5, 10.5, and 9.1% more GY than those plots that did not receive topdress N (Table 2).The assessment of interaction effects of source by at-seeding N rate on GY showed significant (p
<
0.07) effect at Conrad in 2013 and Kalispell in 2011. At Conrad, for 168 kg ha−1 at-seeding N rate, the blend source had 621 kg ha−1 more GY compared with urea and ESN combined. At this site-year, for urea, GY was linearly increased with increase in at-seeding N rate (Table 3(a)). At Kalispell, for 336 kg ha−1 at-seeding N rate, ESN had 563 kg ha−1 more GY than urea. Consistent with Conrad 2013, at Kalispell in 2011, GY decreased linearly with increase in at-seeding N rate from 112 kg ha−1 to 336 kg ha−1. The source by topdress interaction showed significant differences at Conrad in 2011 (p
<
0.016) and 2013 (p
<
0.025). In both years at this site, for each source, topdressing N resulted in more GY than not topdressing although statistically significant LS means difference was observed only for ESN source in 2011 and urea in 2013 (Table 3(a)). At Conrad in 2013, at the 45 kg ha−1 topdress N rate, the blend had 448 kg ha−1 more GY than urea.Table 3
(a) Simple effects of two-way interactions of source by at-seeding and topdress N rates for grain yield in selected site-years where interaction effect was significant at least atp
<
0.1. (b) Simple effects of three-way interactions of source and at-seeding and topdress N rates on grain yield in selected site-years where interaction effect was significant at least at p
<
0.15.
(a)
Site-year
At-seeding N rate, kg ha−1
Blend
ESN
Urea
kg ha−1
Conrad-2013
56
4536
4578
4855
112
4695
4570
4452
168
4838A
†
4200B
4234B
Prob >F (trend)
0.156
0.754
0.019‡
Kalispell-2011
112
3656
4013
3785
224
3878
3875
3603
336
3749A
B
3905A
3342B
Prob >F (trend)
0.745
0.689
0.05‡
Topdress N rate, kg ha−1
Conrad-2011
0
2445
2255b
§
2470
45
2668
2943a
2613
Conrad-2013
0
4648
4379
4743a
45
4732A
4519A
B
4284B
b
†Across rows for each site-year and at-seeding N rate, LS means followed by different letters are significant at p
<
0.05.
‡Significant linear trend for at-seeding N rate for each source.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.
(b)
At-seeding–topdress N rate, kg ha−1
Blend
ESN
Urea
Blend
ESN
Urea
Conrad-2011
Conrad-2013
56–0
2523b
c
§
1812c
2027b
4385b
4301a
b
4755a
56–45
2202c
2268c
2298b
4688a
b
4855
a
4956
a
112–0
2008c
B
†
2480b
c
B
2932
a
A
4620a
b
4486a
b
4805a
112–45
2454b
c
2886b
2885a
4771a
b
A
4654a
b
A
4099b
B
168/0
2804a
b
2474b
c
2450a
b
4939
a
A
4351a
b
B
4670a
b
A
B
168–45
3349
a
A
B
3676
a
A
2656a
b
B
4738a
b
A
4049b
B
3797b
B
Kalispell-2011
112–0
3478b
3808b
3681a
b
112–45
3833a
b
4218
a
3889
a
224–0
3842a
b
3755b
3583a
b
224–45
3915
a
3994a
b
3624a
b
336–0
3625a
b
A
B
3901a
b
A
3431b
B
336–45
3872a
A
3910a
b
A
3253b
B
§Down a column for each source, at-seeding–topdress N rate LS means followed by different lowercase letters are significant at p
<
0.05.
†Across rows for each site-year, at-seeding–topdress N rate combination, source LS means followed by different uppercase letters are significant at p
<
0.05.Results of assessment of three-way interaction effects (p
<
0.15) of source and at-seeding and topdress N rates on GY are presented in Table 3(b). At Conrad the maximum GY was obtained with the highest rate (168 kg ha−1) at-seeding N rate combined with 45 kg ha−1 topdress N for the blend and ESN sources (Table 3(b)). For this site-year, the maximum yield for urea source was achieved with 120 kg ha−1 at-seeding N rate without topdress N. In 2013, at Conrad, the lowest at-seeding N rate combined with 45 kg ha−1 topdress N rate maximized yield for the ESN and urea. In contrast, GY was maximized with the application of 168 N ha−1 at-seeding N rate for the blend source although the values were not statistically different from other rates but the lowest at-seeding rate without topdress. At Kalispell in 2011, similar trend was observed for the ESN and urea where GY was maximized with the low (112 kg ha−1) at-seeding rate plus 45 kg N ha−1 topdress rate. Furthermore, at Kalispell in 2011, GY was maximized with the application of 224 kg ha−1 at-seeding N rate and 45 kg ha−1 topdress N rate combination. But the value was not statistically different from the rest of the rates, except for the low rate without topdress N. Results suggest that low to medium at-seeding N rate along topdres N rate improved GY. In Montana, despite the short spring wheat growing season, yields did increase in this study as a result of topdressing. Splitting N improved yield but this may not translate to positive marginal return. We have found conflicting results from the different site-years regarding the effect of sources and N rates.


### 3.2. Grain Protein Content

In 2011, GP content values ranged from 13.9 to 15.6% at Corvallis, 11.2 to 13.8% at Kalispell, and 9.1 to 9.9% at Conrad. In 2012, excellent GP values were achieved at Kalispell (from 12.8 to 14.6%) and Corvallis (from 12.7 to 13.4%). At Conrad, the GP content was also high, ranging from 9.6 to 13.6%. Grain protein ranged from 14.7 to 17.3% at Corvallis and 11.0 to 14.4% at Conrad in 2013.Grain protein content was significantly affected by N sources at Conrad in all the three years and at Kalispell in 2012 only (Table4). At Conrad in 2011, the ESN and blend sources combined had 0.6% higher GP content than urea. Likewise, in 2013, the ESN source had 0.5% more GP content than the urea source. In contrast, in 2012 at Conrad, urea had 0.9 and 0.4% more GP content than ESN and blend, respectively. At Kalispell in 2012, the ESN and blend sources combined had 0.50% more GP content than urea. Wheat GP has increased with increase in at-seeding N rates at all site-years except at Corvallis in 2012. Accordingly, wheat GP values increased linearly with increase in at-seeding N rate for all site-years where effects were significant (Table 4).Table 4
Effect of at N source and preplant and topdress N rate on spring wheat grain protein Conrad, Corvallis and Kalispell, 2011–2013 growing seasons.
Effect
Conrad
Corvallis
Kalispell
2011
2012
2013
2011
2012
2013
2011
2012
N source, kg ha−1
%
Urea
9.4b
£
12.9a
13.6b
14.8
13.4
16.7
12.6
13.8b
ESN
10.0a
12.0c
14.1a
14.9
12.9
16.8
12.4
14.2a
Blend
9.9a
12.5b
13.8a
b
14.9
12.7
16.8
12.4
14.4a
Prob >F
0.0501
<0.001
0.011
0.732
0.123
0.887
0.656
<0.001
At-seeding N rate, kg ha−1
0
9.1
9.6
11.0
13.9
13.1
14.7
11.2
12.8
Low†
9.1
11.1
13.0
14.4
12.7
16.4
11.7
13.9
Medium
9.3
12.7
14.1
15.6
13.4
16.6
13.1
13.9
High
9.9
13.6
14.4
15.6
13.0
17.3
13.8
14.6
Prob >F (trend)
0.013‡
<0.001‡
<0.001‡
<0.001‡
0.078
0.003‡
<0.001‡
<0.001‡
Topdress N rate, kg ha−1
0
9.4
12.1b
§
13.6b
14.4b
13.0
16.9
12.1b
14.4b
45
9.5
12.9a
14.1a
15.3a
13.0
16.6
12.9a
13.9a
Prob >F
0.918
0.001
0.001
0.001
0.968
0.251
0.001
0.001
£Down a column for each site-year source means followed by different letter are significant at p
<
0.05.
†At-seeding N rates: low, medium, and high were 56, 112, and 168 kg ha−1 for Conrad and 112, 224, and 336 kg ha−1 for Corvallis and Kalispell.
‡Significant linear trend, for at-seeding N rate for each site-year.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.Source by at-seeding N rate interaction was significant at Conrad (p
<
0.02) and Kalispell (p
<
0.001) in 2012. At Conrad GP content increased consistently with increase in at-seeding N rate from 56 kg ha−1 to 168 kg ha−1 for all the three sources. However, a more steep increase was observed for urea than ESN or the blend (Table 5). At Conrad in 2012, at l056 kg ha−1 at-seeding N rate, all sources exhibited low and insignificant GP content (11.1 to 11.2%). At the 112 kg ha−1 at-seeding N rate, GP content for blend and ESN was 1.2 and 0.7% lower than urea, respectively, while the difference was 1.4 and 0.6% for 168 kg ha−1 at-seeding N rate, in the same order.Table 5
Simple effects of two-way interactions of source by at-seeding and topdress N rates for wheat grin protein from selected site-years where interaction effect was significant at least atp
<
0.1.
Site-year
⁢At-seeding N rate, kg ha−1
Source
Blend
ESN
Urea
%
Conrad-2012
56
11.1
11.1
11.2
112
12.1C
†
12.6B
13.3B
168
12.9C
13.7B
14.3A
Prob >F (trend)
0.012‡
0.021‡
0.011‡
Kalispell-2012
112
14.4A
14.3A
13.0B
224
13.7
14.2
13.9
336
14.5
14.8
14.5
Prob >F (trend)
0.023€
0.047€
0.009‡
Topdress N, kg ha−1
Conrad-2012
0
11.6B
b
§
12.2A
b
12.4A
b
45
12.4B
a
12.8B
a
13.5A
a
Corvallis-2011
0
14.7b
14.6b
14.8b
45
15.9A
a
15.8A
B
a
15.4B
a
Corvallis-2012
0
13.2A
a
12.6B
13.2A
b
45
12.5B
b
12.8B
13.7A
a
Corvallis-2013
0
17.2A
a
17.1A
16.4B
b
45
16.4B
b
17.1A
17A
a
Kalispell-2012
0
14.6A
a
14.8A
a
13.8B
45
13.8b
14.1b
13.8
†Across rows for each site-year, at-seeding N rate, or topdress N rate, LS means followed by different uppercase letters are significant at p
<
0.05.
‡  &  € represent significant linear and quadratic trends, for at-seeding N rate for each source.
§Down a column for topdress N rates, LS means followed by different lowercase letters are significant at p
<
0.05 for each source.At Kalispell in 2012, GP content linearly increased with at-seeding N rates for urea but decreased with increasing at-seeding N rate from 112 kg ha−1 t to 224 kg ha−1 and then peaked again with the ESN and blend sources (Table 5). For this site-year, at the 112 kg ha−1 at-seeding N rate, urea had the lowest GP content while ESN and the blend had similar GP content (Table 5).Source by topdress N interaction was significant at Conrad in 2012 (p
<
0.05), at Corvallis in all the three years (p
<
0.02 to p
<
0.06), and at Kalispell in 2012 (p
<
0.001). At Conrad in 2012, without topdress N, ESN and urea combined resulted in 0.7% more GP content than the blend. With topdress N, urea resulted in 1.1 and 0.7% more GP content than the blend and ESN, respectively (Table 5). Additionally, at this site-year, topdress N resulted in 0.8, 0.6, and 1.1% more GP content for the blend, ESN, and urea, respectively, than plots that did not receive topdress N.At Corvallis in 2011, with topdress N, the blend source had 0.5% more GP content than urea. Likewise, at this site-year, consistent across sources, topdressing N resulted in more GP content than no topdressing. Accordingly, topdress N resulted in 1.2, 1.2, and 0.6% more GP content for the blend, ESN, and urea sources, respectively, compared with no topdress.At Corvallis in 2012, the effect of sources on the GP content at each topdress N was inconsistent. For this site-year, both blend and urea resulted in 0.6% more GP content than the ESN source with no topdress N while urea resulted in almost 1% more GP content than blend and ESN combined. For this site-year, for the blend source topdressed plots resulted in 0.7% less GP content than nontopdressed plots; however, when wheat was topdressed with N, 0.5% more GP content was obtained compared to nontopdressed plots (Table5).At Corvallis in 2013, without topdress N, urea had 0.8% less GP content than ESN and blend sources combined whereas, with topdress N, ESN and urea sources resulted in 0.7% more GP content than the blend (Table5). For this site-year, topdress N resulted in 0.8% less and 0.6% more GP content for blend and urea sources, respectively.At Kalispell in 2012, without topdress N, blend and ESN sources resulted in 1.2 and 1.0% more GP content than urea, respectively. Furthermore, for this site-year, when sources were blend and ESN, topdressed plots had 0.8 and 0.7% more GP content than plots that did not receive topdress N.


### 3.3. Nitrogen Uptake

Nitrogen source significantly affected GNU only at Corvallis and Kalispell in 2011 (Table6). At Corvallis, urea resulted in 16.3 kg ha−1 more GNU than ESN and blend combined. In contrast, ESN had 7.8 kg ha−1 more GNU than urea at Kalispell in 2011.Table 6
Effect of at N source, preplant and topdress N rate on spring wheat grain uptake Conrad, Corvallis and Kalispell, 2011–2013 growing seasons.
Effect
Conrad
Corvallis
Kalispell
2011
2012
2013
2011
2012
2013
2011
2012
N source
kg ha−1
Urea
51.6
117.7
106.5
96.4a
£
106.5
72.9
99.8b
106.5
ESN
54.9
114.3
107.6
78.5b
107.6
71.7
104.3a
b
105.4
Blend
54.9
116.6
109.9
81.8b
102.0
67.3
107.6a
107.6
Prob >F
0.620
0.328
0.401
<0.001
0.664
0.853
0.011
0.782
At-seeding N rate
0
44.8
86.3
68.4
65.0
86.3
46.0
53.8
85.2
Low†
43.7
98.7
104.3
77.4
94.2
71.7
96.4
104.3
Medium
53.8
118.8
111.0
88.6
116.6
77.4
106.5
104.3
High
62.8
131.2
108.7
91.9
105.4
62.8
108.7
109.9
Prob >F
<0.001‡
<0.001‡
0.047‡
0.001‡
0.008€
0.395
<0.001‡
0.051‡
Topdress N rate
0
50.5b
§
112.1b
106.5
79.6b
99.8
70.62
98.7b
107.6
45
57.2a
120.0a
108.7
91.9a
111.0
70.6
109.9a
105.4
Prob >F
0.049
<0.001
0.321
<0.001
<0.001
0.932
0.001
0.204
£Down a column for each site-year source means followed by different letter are significant at p
<
0.05.
†At-seeding N rates: low, medium, and high were 56, 112, and 168 kg ha−1 for Conrad and 112, 224, and 336 kg ha−1 for Corvallis and Kalispell.
‡  &  € represent significant linear and quadratic trends, for at-seeding N rate for each site-year.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.At-seeding N rate treatments significantly affected GNU at all site-years except at Corvallis 2013 (Table6). Grain N uptake increased linearly with increase in at-seeding N rate at all site-years where the effect was significant except at Corvallis 2012. At Corvallis in 2012, a quadratic trend was observed where GNU peaked at 224 kg ha−1 at-seeding N rate. Likewise, plots supplemented with topdress N resulted in higher GNU than nontopdressed plots consistently across site-years where the effect was significant (Table 6).Source by at-seeding N rate interaction was significant at Conrad in 2011 (p
<
0.05) and 2013 (0.021) and at Kalispell (p
<
0.021) in 2011. At Conrad GNU increased linearly with increase in at-seeding N rate for blend and ESN. In contrast, GNU was increased to 41.5 kg ha−1 at 112 kg ha−1 at-seeding N rate and decreased to 37.3 kg ha−1 with 168 kg N ha−1 at-seeding N rate for urea source (Table 7). Additionally, at the 112 and 168 kg ha−1 at-seeding N rates, sources showed significantly different response. Accordingly, at 112 kg ha−1 at-seeding N rate, blend had 8.5 and 8.8 less GNU than ESN and urea, respectively. In contrast, at 168 kg ha−1 at-seeding N rate, blend and ESN had 12.4 and 10.3 more GNU than urea, respectively (Table 7).Table 7
Simple effects of two-way interactions of source by at-seeding and topdress N rates for N uptake from selected site-years where interaction effect was significant at least atp
<
0.1.
Site-year
⁢At-seeding N rate, kg ha−1
Source
Blend
ESN
Urea
kg ha−1
Conrad-2011
56
34.6
29.4
30.3
112
32.7B
†
41.2A
41.5
168
49.7A
47.6A
B
37.3B
Prob >F (trend)
0.05‡
0.008‡
0.045€
Conrad-2013
56
89.5
94
95.2
112
100.1A
100.7B
96.5B
168
104.8B
93.9A
94.5A
B
Prob >F (trend)
0.022‡
0.165
0.754
Kalispell-2011
112
64A
74.1A
B
68.7B
224
77.7
77.1
73.5
336
81.5A
80.2A
71.5B
Prob >F (trend)
0.007‡
0.068‡
0.171
Topdress N, kgh
a
-
1
§
Conrad-2013
0
94.9b
93.1b
99.1a
45
101.4A
a
99.3A
a
90.7B
b
†Across rows for each site-year, at-seeding N rate, or topdress N rate, LS means followed by different uppercase letters are significant at p
<
0.05.
‡ represents significant linear trend, for at-seeding N rate for each site-year.
§ Down a column for topdress N rates, LS means followed by different lowercase letters are significant at p
<
0.05 for each source.At Conrad in 2013, for the blend source, GNU increased linearly with increase in at-seeding N rate. At this site-year, at 168 kg ha−1 at-seeding N rate, the blend source had 10.6 kg ha−1 more GNU than ESN and urea combined. At Kalispell in 2011, GNU increased linearly with at-seeding N rate from 64.0 to 81.5 kg ha−1 when at-seeding N rate increased from 112 to 336 kg ha−1 for the blend source. At this site-year, at 112 kg ha−1 at-seeding N rate, ESN had 10.1 kg ha−1 more GNU than ESN. At 336 kg ha−1 at-seeding N rate, blend and ESN had 11.0 and 8.7 kg ha−1 more GNU than urea.Significant source by topdress N rate interaction was observed only at Conrad in 2013. Accordingly, topdressing N increased GNU by 6.5 and 6.2 kg ha−1 compared with no topdressing. Alternatively, for urea GNU was decreased by 8.4 kg ha−1 in plots that received topdress N compared with that not receiving N.


### 3.4. Partial Budget Economic Analysis

Only three site-years, Conrad and Corvallis in 2012 and Kalispell in 2011, were considered for the economic analysis based on significant differences in GY (Table8(a)). Net returns for GP-unadjusted revenue were higher for the blend source at Conrad in 2012 (Table 8(a)).Table 8
(a) Protein-unadjusted and -adjusted revenues and marginal returns for selected site-years where grain yield was significant. (b) Protein-unadjusted and -adjusted partial budget analysis comparing medium at-seeding rate (112 and 224 N kg ha−1 at Conrad and Kalispell, resp.) and low at-seeding rate (56 and 112 kg ha−1 at Conrad and Kalispell, resp.) along 45 kg ha−1 topdress N rates for selected site-years.
(a)
Site-year
Unadjusted for protein
Adjusted for protein
Urea
ESN
Blend
Urea
ESN
Blend
Net return,$ ha−1
Conrad-2012
648.79
650.27
669.31
536.35
448.05
529.48
Corvallis-2012
243.93
74.26
126.42
209.16
15.08
55.68
Kalispell-2011
335.39
280.13
343.68
240.45
165.70
225.31
Marginal profit,$ ha−1
Conrad-2012
—
1.48
20.50
—
−88.30
−6.85
Corvallis-2012
—
−169.67
−117.51
—
−194.08
−153.50
Kalispell-2011
—
−55.26
8.29
—
−74.75
−15.13
Marginal rate of return,$ recovery $
-
1 investment
Conrad-2012
—
1.02
1.56
—
−0.31
0.81
Corvallis-2012
—
−0.89
−1.41
—
−1.17
−2.14
Kalispell-2011
—
0.38
1.17
—
0.17
0.69
(b)
Site-year
Unadjusted for protein
Adjusted GY for protein
Urea
ESN
Urea
ESN
Net return,$ ha−1
Conrad-2011
612.68
134.32
502.94
43.38
Conrad-2013
533.88
374.48
479.36
276.45
Kalispell-2011
352.49
255.31
252.67
145.16
Marginal profit,$ ha−1
Conrad-2011
—
−426.36
−459.56
Conrad-2013
—
−85.00
−202.91
Kalispell-2011
—
−22.78
−107.51
Marginal rate of return,$ recovery $
-
1 investment
Conrad-2011
—
−8.20
−7.84
Conrad-2013
—
−1.14
−1.73
Kalispell-2011
—
−0.31
−0.45A slight revenue advantage was obtained with the blend at Kalispell. At Corvallis, urea outperformed both the ESN and blend. In fact, ESN performed poorly in all the three site-ears. At Conrad in 2012, ESN had lower marginal net return than blend ($19.02 ha−1 more). At Kalispell, a similar result was observed where the blend resulted in $8.29 ha−1 additional income compared to urea. At Conrad, MRR was $1.02 and 1.56 for ESN and blend, respectively, while at Kalispell the MRR was 0.38 and 1.17 for GP-unadjusted revenue. At Corvallis 2012, negative marginal rate of return was observed for both ESN and blend sources.Similarly, protein-unadjusted and -adjusted partial budget analysis comparing medium at-seeding rate and low at-seeding rate along 45 kg ha−1 topdress N rates showed that ESN did not result in better marginal profit or marginal rate of return compared with urea at the specified rates (Table 8(b)).


## 3.1. Grain Yield

In 2011, the lowest GY was observed at Conrad (2488 kg ha−1), followed by Corvallis (2623 to 3161 kg ha−1). Grain yield at Kalispell was the highest among the three experimental sites, ranging from 2898 to 4237 kg ha−1. In 2012, GY was the highest among the three growing seasons for all experimental locations. Conrad was the highest producing site with GY ranging from 4035 to 5583 kg ha−1. The GY for Kalispell and Corvallis was comparable but slightly higher at Corvallis. In 2013, GY was higher at Conrad (3699 to 4640 kg ha−1) than Corvallis (1816 to 2623 kg ha−1).Nitrogen source affected GY significantly at Conrad in 2012 and Corvallis and Kalispell in 2011 (Table2). At Conrad, ESN surpassed urea by 405 kg ha−1. At Corvallis, urea resulted in 16% more GY than ESN and blend combined. In 2011, at Kalispell, the blend yielded 336 kg ha−1 more compared to urea alone (Table 2). Overall, urea-ESN blend resulted in a significantly higher GY at Kalispell.At-seeding N rate significantly affected wheat GY at Conrad in 2011 and 2012 and at Corvallis in 2012 (Table2). Wheat GY increased linearly from 2488 to 3161 kg ha−1 in 2011 and from 4035 kg ha−1 to 5582 kg ha−1 in 2012 at Conrad when at-seeding N rate increased from 0 to 168 kg ha−1. Similarly, a linear increase from 2623 to 3026 kg ha−1 was observed in 2011 at Corvallis when at-seeding N rate increased from 0 to 336 kg ha−1 (Table 2). A quadratic trend was observed at Corvallis in 2012 and 2013 where GY reached peak at the medium N rate (224 kg ha−1).The topdress N treatment significantly affected GY in four of the eight site-years. At Conrad in 2011, Corvallis in 2011 and 2012, and Kalispell 2011, topdressing 45 kg N ha−1 resulted in 14.7, 12.5, 10.5, and 9.1% more GY than those plots that did not receive topdress N (Table 2).The assessment of interaction effects of source by at-seeding N rate on GY showed significant (p
<
0.07) effect at Conrad in 2013 and Kalispell in 2011. At Conrad, for 168 kg ha−1 at-seeding N rate, the blend source had 621 kg ha−1 more GY compared with urea and ESN combined. At this site-year, for urea, GY was linearly increased with increase in at-seeding N rate (Table 3(a)). At Kalispell, for 336 kg ha−1 at-seeding N rate, ESN had 563 kg ha−1 more GY than urea. Consistent with Conrad 2013, at Kalispell in 2011, GY decreased linearly with increase in at-seeding N rate from 112 kg ha−1 to 336 kg ha−1. The source by topdress interaction showed significant differences at Conrad in 2011 (p
<
0.016) and 2013 (p
<
0.025). In both years at this site, for each source, topdressing N resulted in more GY than not topdressing although statistically significant LS means difference was observed only for ESN source in 2011 and urea in 2013 (Table 3(a)). At Conrad in 2013, at the 45 kg ha−1 topdress N rate, the blend had 448 kg ha−1 more GY than urea.Table 3
(a) Simple effects of two-way interactions of source by at-seeding and topdress N rates for grain yield in selected site-years where interaction effect was significant at least atp
<
0.1. (b) Simple effects of three-way interactions of source and at-seeding and topdress N rates on grain yield in selected site-years where interaction effect was significant at least at p
<
0.15.
(a)
Site-year
At-seeding N rate, kg ha−1
Blend
ESN
Urea
kg ha−1
Conrad-2013
56
4536
4578
4855
112
4695
4570
4452
168
4838A
†
4200B
4234B
Prob >F (trend)
0.156
0.754
0.019‡
Kalispell-2011
112
3656
4013
3785
224
3878
3875
3603
336
3749A
B
3905A
3342B
Prob >F (trend)
0.745
0.689
0.05‡
Topdress N rate, kg ha−1
Conrad-2011
0
2445
2255b
§
2470
45
2668
2943a
2613
Conrad-2013
0
4648
4379
4743a
45
4732A
4519A
B
4284B
b
†Across rows for each site-year and at-seeding N rate, LS means followed by different letters are significant at p
<
0.05.
‡Significant linear trend for at-seeding N rate for each source.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.
(b)
At-seeding–topdress N rate, kg ha−1
Blend
ESN
Urea
Blend
ESN
Urea
Conrad-2011
Conrad-2013
56–0
2523b
c
§
1812c
2027b
4385b
4301a
b
4755a
56–45
2202c
2268c
2298b
4688a
b
4855
a
4956
a
112–0
2008c
B
†
2480b
c
B
2932
a
A
4620a
b
4486a
b
4805a
112–45
2454b
c
2886b
2885a
4771a
b
A
4654a
b
A
4099b
B
168/0
2804a
b
2474b
c
2450a
b
4939
a
A
4351a
b
B
4670a
b
A
B
168–45
3349
a
A
B
3676
a
A
2656a
b
B
4738a
b
A
4049b
B
3797b
B
Kalispell-2011
112–0
3478b
3808b
3681a
b
112–45
3833a
b
4218
a
3889
a
224–0
3842a
b
3755b
3583a
b
224–45
3915
a
3994a
b
3624a
b
336–0
3625a
b
A
B
3901a
b
A
3431b
B
336–45
3872a
A
3910a
b
A
3253b
B
§Down a column for each source, at-seeding–topdress N rate LS means followed by different lowercase letters are significant at p
<
0.05.
†Across rows for each site-year, at-seeding–topdress N rate combination, source LS means followed by different uppercase letters are significant at p
<
0.05.Results of assessment of three-way interaction effects (p
<
0.15) of source and at-seeding and topdress N rates on GY are presented in Table 3(b). At Conrad the maximum GY was obtained with the highest rate (168 kg ha−1) at-seeding N rate combined with 45 kg ha−1 topdress N for the blend and ESN sources (Table 3(b)). For this site-year, the maximum yield for urea source was achieved with 120 kg ha−1 at-seeding N rate without topdress N. In 2013, at Conrad, the lowest at-seeding N rate combined with 45 kg ha−1 topdress N rate maximized yield for the ESN and urea. In contrast, GY was maximized with the application of 168 N ha−1 at-seeding N rate for the blend source although the values were not statistically different from other rates but the lowest at-seeding rate without topdress. At Kalispell in 2011, similar trend was observed for the ESN and urea where GY was maximized with the low (112 kg ha−1) at-seeding rate plus 45 kg N ha−1 topdress rate. Furthermore, at Kalispell in 2011, GY was maximized with the application of 224 kg ha−1 at-seeding N rate and 45 kg ha−1 topdress N rate combination. But the value was not statistically different from the rest of the rates, except for the low rate without topdress N. Results suggest that low to medium at-seeding N rate along topdres N rate improved GY. In Montana, despite the short spring wheat growing season, yields did increase in this study as a result of topdressing. Splitting N improved yield but this may not translate to positive marginal return. We have found conflicting results from the different site-years regarding the effect of sources and N rates.


## 3.2. Grain Protein Content

In 2011, GP content values ranged from 13.9 to 15.6% at Corvallis, 11.2 to 13.8% at Kalispell, and 9.1 to 9.9% at Conrad. In 2012, excellent GP values were achieved at Kalispell (from 12.8 to 14.6%) and Corvallis (from 12.7 to 13.4%). At Conrad, the GP content was also high, ranging from 9.6 to 13.6%. Grain protein ranged from 14.7 to 17.3% at Corvallis and 11.0 to 14.4% at Conrad in 2013.Grain protein content was significantly affected by N sources at Conrad in all the three years and at Kalispell in 2012 only (Table4). At Conrad in 2011, the ESN and blend sources combined had 0.6% higher GP content than urea. Likewise, in 2013, the ESN source had 0.5% more GP content than the urea source. In contrast, in 2012 at Conrad, urea had 0.9 and 0.4% more GP content than ESN and blend, respectively. At Kalispell in 2012, the ESN and blend sources combined had 0.50% more GP content than urea. Wheat GP has increased with increase in at-seeding N rates at all site-years except at Corvallis in 2012. Accordingly, wheat GP values increased linearly with increase in at-seeding N rate for all site-years where effects were significant (Table 4).Table 4
Effect of at N source and preplant and topdress N rate on spring wheat grain protein Conrad, Corvallis and Kalispell, 2011–2013 growing seasons.
Effect
Conrad
Corvallis
Kalispell
2011
2012
2013
2011
2012
2013
2011
2012
N source, kg ha−1
%
Urea
9.4b
£
12.9a
13.6b
14.8
13.4
16.7
12.6
13.8b
ESN
10.0a
12.0c
14.1a
14.9
12.9
16.8
12.4
14.2a
Blend
9.9a
12.5b
13.8a
b
14.9
12.7
16.8
12.4
14.4a
Prob >F
0.0501
<0.001
0.011
0.732
0.123
0.887
0.656
<0.001
At-seeding N rate, kg ha−1
0
9.1
9.6
11.0
13.9
13.1
14.7
11.2
12.8
Low†
9.1
11.1
13.0
14.4
12.7
16.4
11.7
13.9
Medium
9.3
12.7
14.1
15.6
13.4
16.6
13.1
13.9
High
9.9
13.6
14.4
15.6
13.0
17.3
13.8
14.6
Prob >F (trend)
0.013‡
<0.001‡
<0.001‡
<0.001‡
0.078
0.003‡
<0.001‡
<0.001‡
Topdress N rate, kg ha−1
0
9.4
12.1b
§
13.6b
14.4b
13.0
16.9
12.1b
14.4b
45
9.5
12.9a
14.1a
15.3a
13.0
16.6
12.9a
13.9a
Prob >F
0.918
0.001
0.001
0.001
0.968
0.251
0.001
0.001
£Down a column for each site-year source means followed by different letter are significant at p
<
0.05.
†At-seeding N rates: low, medium, and high were 56, 112, and 168 kg ha−1 for Conrad and 112, 224, and 336 kg ha−1 for Corvallis and Kalispell.
‡Significant linear trend, for at-seeding N rate for each site-year.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.Source by at-seeding N rate interaction was significant at Conrad (p
<
0.02) and Kalispell (p
<
0.001) in 2012. At Conrad GP content increased consistently with increase in at-seeding N rate from 56 kg ha−1 to 168 kg ha−1 for all the three sources. However, a more steep increase was observed for urea than ESN or the blend (Table 5). At Conrad in 2012, at l056 kg ha−1 at-seeding N rate, all sources exhibited low and insignificant GP content (11.1 to 11.2%). At the 112 kg ha−1 at-seeding N rate, GP content for blend and ESN was 1.2 and 0.7% lower than urea, respectively, while the difference was 1.4 and 0.6% for 168 kg ha−1 at-seeding N rate, in the same order.Table 5
Simple effects of two-way interactions of source by at-seeding and topdress N rates for wheat grin protein from selected site-years where interaction effect was significant at least atp
<
0.1.
Site-year
⁢At-seeding N rate, kg ha−1
Source
Blend
ESN
Urea
%
Conrad-2012
56
11.1
11.1
11.2
112
12.1C
†
12.6B
13.3B
168
12.9C
13.7B
14.3A
Prob >F (trend)
0.012‡
0.021‡
0.011‡
Kalispell-2012
112
14.4A
14.3A
13.0B
224
13.7
14.2
13.9
336
14.5
14.8
14.5
Prob >F (trend)
0.023€
0.047€
0.009‡
Topdress N, kg ha−1
Conrad-2012
0
11.6B
b
§
12.2A
b
12.4A
b
45
12.4B
a
12.8B
a
13.5A
a
Corvallis-2011
0
14.7b
14.6b
14.8b
45
15.9A
a
15.8A
B
a
15.4B
a
Corvallis-2012
0
13.2A
a
12.6B
13.2A
b
45
12.5B
b
12.8B
13.7A
a
Corvallis-2013
0
17.2A
a
17.1A
16.4B
b
45
16.4B
b
17.1A
17A
a
Kalispell-2012
0
14.6A
a
14.8A
a
13.8B
45
13.8b
14.1b
13.8
†Across rows for each site-year, at-seeding N rate, or topdress N rate, LS means followed by different uppercase letters are significant at p
<
0.05.
‡  &  € represent significant linear and quadratic trends, for at-seeding N rate for each source.
§Down a column for topdress N rates, LS means followed by different lowercase letters are significant at p
<
0.05 for each source.At Kalispell in 2012, GP content linearly increased with at-seeding N rates for urea but decreased with increasing at-seeding N rate from 112 kg ha−1 t to 224 kg ha−1 and then peaked again with the ESN and blend sources (Table 5). For this site-year, at the 112 kg ha−1 at-seeding N rate, urea had the lowest GP content while ESN and the blend had similar GP content (Table 5).Source by topdress N interaction was significant at Conrad in 2012 (p
<
0.05), at Corvallis in all the three years (p
<
0.02 to p
<
0.06), and at Kalispell in 2012 (p
<
0.001). At Conrad in 2012, without topdress N, ESN and urea combined resulted in 0.7% more GP content than the blend. With topdress N, urea resulted in 1.1 and 0.7% more GP content than the blend and ESN, respectively (Table 5). Additionally, at this site-year, topdress N resulted in 0.8, 0.6, and 1.1% more GP content for the blend, ESN, and urea, respectively, than plots that did not receive topdress N.At Corvallis in 2011, with topdress N, the blend source had 0.5% more GP content than urea. Likewise, at this site-year, consistent across sources, topdressing N resulted in more GP content than no topdressing. Accordingly, topdress N resulted in 1.2, 1.2, and 0.6% more GP content for the blend, ESN, and urea sources, respectively, compared with no topdress.At Corvallis in 2012, the effect of sources on the GP content at each topdress N was inconsistent. For this site-year, both blend and urea resulted in 0.6% more GP content than the ESN source with no topdress N while urea resulted in almost 1% more GP content than blend and ESN combined. For this site-year, for the blend source topdressed plots resulted in 0.7% less GP content than nontopdressed plots; however, when wheat was topdressed with N, 0.5% more GP content was obtained compared to nontopdressed plots (Table5).At Corvallis in 2013, without topdress N, urea had 0.8% less GP content than ESN and blend sources combined whereas, with topdress N, ESN and urea sources resulted in 0.7% more GP content than the blend (Table5). For this site-year, topdress N resulted in 0.8% less and 0.6% more GP content for blend and urea sources, respectively.At Kalispell in 2012, without topdress N, blend and ESN sources resulted in 1.2 and 1.0% more GP content than urea, respectively. Furthermore, for this site-year, when sources were blend and ESN, topdressed plots had 0.8 and 0.7% more GP content than plots that did not receive topdress N.


## 3.3. Nitrogen Uptake

Nitrogen source significantly affected GNU only at Corvallis and Kalispell in 2011 (Table6). At Corvallis, urea resulted in 16.3 kg ha−1 more GNU than ESN and blend combined. In contrast, ESN had 7.8 kg ha−1 more GNU than urea at Kalispell in 2011.Table 6
Effect of at N source, preplant and topdress N rate on spring wheat grain uptake Conrad, Corvallis and Kalispell, 2011–2013 growing seasons.
Effect
Conrad
Corvallis
Kalispell
2011
2012
2013
2011
2012
2013
2011
2012
N source
kg ha−1
Urea
51.6
117.7
106.5
96.4a
£
106.5
72.9
99.8b
106.5
ESN
54.9
114.3
107.6
78.5b
107.6
71.7
104.3a
b
105.4
Blend
54.9
116.6
109.9
81.8b
102.0
67.3
107.6a
107.6
Prob >F
0.620
0.328
0.401
<0.001
0.664
0.853
0.011
0.782
At-seeding N rate
0
44.8
86.3
68.4
65.0
86.3
46.0
53.8
85.2
Low†
43.7
98.7
104.3
77.4
94.2
71.7
96.4
104.3
Medium
53.8
118.8
111.0
88.6
116.6
77.4
106.5
104.3
High
62.8
131.2
108.7
91.9
105.4
62.8
108.7
109.9
Prob >F
<0.001‡
<0.001‡
0.047‡
0.001‡
0.008€
0.395
<0.001‡
0.051‡
Topdress N rate
0
50.5b
§
112.1b
106.5
79.6b
99.8
70.62
98.7b
107.6
45
57.2a
120.0a
108.7
91.9a
111.0
70.6
109.9a
105.4
Prob >F
0.049
<0.001
0.321
<0.001
<0.001
0.932
0.001
0.204
£Down a column for each site-year source means followed by different letter are significant at p
<
0.05.
†At-seeding N rates: low, medium, and high were 56, 112, and 168 kg ha−1 for Conrad and 112, 224, and 336 kg ha−1 for Corvallis and Kalispell.
‡  &  € represent significant linear and quadratic trends, for at-seeding N rate for each site-year.
§Down a column for topdress N rates, LS means followed by different letters are significant at p
<
0.05 for each source.At-seeding N rate treatments significantly affected GNU at all site-years except at Corvallis 2013 (Table6). Grain N uptake increased linearly with increase in at-seeding N rate at all site-years where the effect was significant except at Corvallis 2012. At Corvallis in 2012, a quadratic trend was observed where GNU peaked at 224 kg ha−1 at-seeding N rate. Likewise, plots supplemented with topdress N resulted in higher GNU than nontopdressed plots consistently across site-years where the effect was significant (Table 6).Source by at-seeding N rate interaction was significant at Conrad in 2011 (p
<
0.05) and 2013 (0.021) and at Kalispell (p
<
0.021) in 2011. At Conrad GNU increased linearly with increase in at-seeding N rate for blend and ESN. In contrast, GNU was increased to 41.5 kg ha−1 at 112 kg ha−1 at-seeding N rate and decreased to 37.3 kg ha−1 with 168 kg N ha−1 at-seeding N rate for urea source (Table 7). Additionally, at the 112 and 168 kg ha−1 at-seeding N rates, sources showed significantly different response. Accordingly, at 112 kg ha−1 at-seeding N rate, blend had 8.5 and 8.8 less GNU than ESN and urea, respectively. In contrast, at 168 kg ha−1 at-seeding N rate, blend and ESN had 12.4 and 10.3 more GNU than urea, respectively (Table 7).Table 7
Simple effects of two-way interactions of source by at-seeding and topdress N rates for N uptake from selected site-years where interaction effect was significant at least atp
<
0.1.
Site-year
⁢At-seeding N rate, kg ha−1
Source
Blend
ESN
Urea
kg ha−1
Conrad-2011
56
34.6
29.4
30.3
112
32.7B
†
41.2A
41.5
168
49.7A
47.6A
B
37.3B
Prob >F (trend)
0.05‡
0.008‡
0.045€
Conrad-2013
56
89.5
94
95.2
112
100.1A
100.7B
96.5B
168
104.8B
93.9A
94.5A
B
Prob >F (trend)
0.022‡
0.165
0.754
Kalispell-2011
112
64A
74.1A
B
68.7B
224
77.7
77.1
73.5
336
81.5A
80.2A
71.5B
Prob >F (trend)
0.007‡
0.068‡
0.171
Topdress N, kgh
a
-
1
§
Conrad-2013
0
94.9b
93.1b
99.1a
45
101.4A
a
99.3A
a
90.7B
b
†Across rows for each site-year, at-seeding N rate, or topdress N rate, LS means followed by different uppercase letters are significant at p
<
0.05.
‡ represents significant linear trend, for at-seeding N rate for each site-year.
§ Down a column for topdress N rates, LS means followed by different lowercase letters are significant at p
<
0.05 for each source.At Conrad in 2013, for the blend source, GNU increased linearly with increase in at-seeding N rate. At this site-year, at 168 kg ha−1 at-seeding N rate, the blend source had 10.6 kg ha−1 more GNU than ESN and urea combined. At Kalispell in 2011, GNU increased linearly with at-seeding N rate from 64.0 to 81.5 kg ha−1 when at-seeding N rate increased from 112 to 336 kg ha−1 for the blend source. At this site-year, at 112 kg ha−1 at-seeding N rate, ESN had 10.1 kg ha−1 more GNU than ESN. At 336 kg ha−1 at-seeding N rate, blend and ESN had 11.0 and 8.7 kg ha−1 more GNU than urea.Significant source by topdress N rate interaction was observed only at Conrad in 2013. Accordingly, topdressing N increased GNU by 6.5 and 6.2 kg ha−1 compared with no topdressing. Alternatively, for urea GNU was decreased by 8.4 kg ha−1 in plots that received topdress N compared with that not receiving N.


## 3.4. Partial Budget Economic Analysis

Only three site-years, Conrad and Corvallis in 2012 and Kalispell in 2011, were considered for the economic analysis based on significant differences in GY (Table8(a)). Net returns for GP-unadjusted revenue were higher for the blend source at Conrad in 2012 (Table 8(a)).Table 8
(a) Protein-unadjusted and -adjusted revenues and marginal returns for selected site-years where grain yield was significant. (b) Protein-unadjusted and -adjusted partial budget analysis comparing medium at-seeding rate (112 and 224 N kg ha−1 at Conrad and Kalispell, resp.) and low at-seeding rate (56 and 112 kg ha−1 at Conrad and Kalispell, resp.) along 45 kg ha−1 topdress N rates for selected site-years.
(a)
Site-year
Unadjusted for protein
Adjusted for protein
Urea
ESN
Blend
Urea
ESN
Blend
Net return,$ ha−1
Conrad-2012
648.79
650.27
669.31
536.35
448.05
529.48
Corvallis-2012
243.93
74.26
126.42
209.16
15.08
55.68
Kalispell-2011
335.39
280.13
343.68
240.45
165.70
225.31
Marginal profit,$ ha−1
Conrad-2012
—
1.48
20.50
—
−88.30
−6.85
Corvallis-2012
—
−169.67
−117.51
—
−194.08
−153.50
Kalispell-2011
—
−55.26
8.29
—
−74.75
−15.13
Marginal rate of return,$ recovery $
-
1 investment
Conrad-2012
—
1.02
1.56
—
−0.31
0.81
Corvallis-2012
—
−0.89
−1.41
—
−1.17
−2.14
Kalispell-2011
—
0.38
1.17
—
0.17
0.69
(b)
Site-year
Unadjusted for protein
Adjusted GY for protein
Urea
ESN
Urea
ESN
Net return,$ ha−1
Conrad-2011
612.68
134.32
502.94
43.38
Conrad-2013
533.88
374.48
479.36
276.45
Kalispell-2011
352.49
255.31
252.67
145.16
Marginal profit,$ ha−1
Conrad-2011
—
−426.36
−459.56
Conrad-2013
—
−85.00
−202.91
Kalispell-2011
—
−22.78
−107.51
Marginal rate of return,$ recovery $
-
1 investment
Conrad-2011
—
−8.20
−7.84
Conrad-2013
—
−1.14
−1.73
Kalispell-2011
—
−0.31
−0.45A slight revenue advantage was obtained with the blend at Kalispell. At Corvallis, urea outperformed both the ESN and blend. In fact, ESN performed poorly in all the three site-ears. At Conrad in 2012, ESN had lower marginal net return than blend ($19.02 ha−1 more). At Kalispell, a similar result was observed where the blend resulted in $8.29 ha−1 additional income compared to urea. At Conrad, MRR was $1.02 and 1.56 for ESN and blend, respectively, while at Kalispell the MRR was 0.38 and 1.17 for GP-unadjusted revenue. At Corvallis 2012, negative marginal rate of return was observed for both ESN and blend sources.Similarly, protein-unadjusted and -adjusted partial budget analysis comparing medium at-seeding rate and low at-seeding rate along 45 kg ha−1 topdress N rates showed that ESN did not result in better marginal profit or marginal rate of return compared with urea at the specified rates (Table 8(b)).


## 4. Discussion and Conclusion

This study has enabled us to evaluate the N source effect on GY and GP in a wide range of growing environments. Average spring wheat GY has varied from 1816 to 5583 kg ha−1 (Table 2) and GP content ranged from 9.1 to 17.3% among the site-years (Table 4).Spring wheat GY responded to N applied at the time of seeding at seven of eight site-years (Table2); also higher GP content was achieved with at-seeding N application (Table 4). These results emphasize the importance of initial N fertilizer application for wheat stand establishment and early-season crop development. The fact that only three out of eight site-years have benefited from the application of a high N rate at seeding suggests that the current university guidelines for spring wheat are too high for the typically observed GYs for the area. This is further reinforced by the finding that GY had not responded to at-seeding N application rate above the lowest rate at four out of eight site-years (Table 2). Furthermore, in four of eight site-years, GY was significantly increased with topdress N application (Table 2). Current work is underway at Montana State University to revise N fertilizer recommendations and adjust them to newly released varieties.The 45 kg N ha−1 rate of topdress used in this study is a typically recommended rate for spring wheat grown in Montana for growers aiming to enhance GP values. The GP content was increased with topdress application of N in five of eight site-years (Table 4). This shows the significance of topdressing N to boost GP content that essentially improves wheat quality.No consistent trend in GY associated with N source was observed in this study: out of eight site-years, urea resulted in higher GYs at three site-years, ESN produced higher GYs at three site-years, and the blend produced higher GYs at two site-years (with 1 site-year having no data available for the blend) (Table2). These results are in agreement with previous findings [46, 47] who observed that ESN and blend have outperformed urea in some years but resulted in lower crop GY in other years.No significant differences in GP content associated with N source were observed in most site-years; ESN and blend resulted in higher GP content compared to urea alone at three of eight site-years evaluated (Table4). While preplant or at-seeding applications of slow-release N products like ESN may be advantageous in winter wheat, it does not always result in improved GP in spring wheat. This may be mainly due to insufficient time for N release during shorter growing season.Nitrogen uptake increased with increase in at-seeding N rates at seven of eight site-years; topdress N application increased N uptake in five of eight site-years (Table6).Nitrogen uptake was similar for all N sources, except for one site-year where slight increase in N uptake was observed with the blend application and for one site-year where the blend resulted in significantly greater N uptake (Table6). It is possible that similar GY results achieved with urea, ESN, and blend were obtained because similar N losses (predominantly due to ammonia volatilization) have occurred from urea and ESN. As the soil temperatures increased, the rate of urea hydrolysis from the urea treatments increased [48]. At the same time, the dissolution of urea and its release from the ESN capsules has also increased with higher soil temperatures.This is where the controlled release versus delayed release discussion comes in, as an example; with average soil temperatures in Montana being approximately 68°F for June and July [29] it is expected that most of the urea has been released into the soil. In reality, we can expect comparable losses of N to occur from both urea and ESN treatments via ammonia volatilization. Thus, similar N amounts were available to wheat crop from all N sources applied.The manufacturer’s general recommendation for utilization of ESN for spring wheat in Great Plains region is application of N as 100% ESN in the fall prior to seeding wheat the next spring [49]. Another commonly recommended application scenario is a spring application as a blend (40–75% ESN + 25–60% urea). Our findings showed that ESN and urea-ESN blend performed well, but not better than urea alone. This is also supported by the partial budget economic analysis results. With GP-unadjusted revenue, by adopting ESN alone as N source, a farmer would lose money on investment but would recover costs by using urea-ESN blend. With GP-adjusted revenue, farmer would not recover investment costs from ESN or blend compared with urea. The losses associated with ESN fertilization strategy make it uneconomical for spring wheat in dryland or irrigated spring wheat production system given the assumptions of the partial budget analysis.Farmers need to be cautious when adopting ESN for improving economic return although environmentally the ESN may be beneficial. An important aspect of fertilizer management strategy is to carefully assess growing conditions that might affect profitability of fertilization.



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*Source: 8969513-2016-06-06.xml*