# Evaluation of the Cytotoxic Effect of Rutin Prenanoemulsion in Lung and Colon Cancer Cell Lines

Authors: Tran Thanh Hoai; Phan Thi Yen; Tran Thi Bich Dao; Luu Hai Long; Duong Xuan Anh; Luu Hai Minh; Bui Quoc Anh; Nghiem Thi Thuong
Journal: Journal of Nanomaterials
 (2020)

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## Abstract

In this work, prenanoemulsion of rutin was prepared using PEG and Tween as emulsifiers via homogenization and evaporation techniques. The particle size of rutin was investigated with high-resolution transmission electron microscopy (HR-TEM); particle size distribution and its chemical structure were analysed by nuclear magnetic resonance (NMR) and Fourier transformed infrared (FT-IR) spectroscopy. It was found that rutin in the prenanoemulsion has excellent solubility in water with the size approximately 15 nm. The chemical structure of nanorutin in prenanoemulsion was identical to that of the pure rutin. It suggested that there is no chemical modification of rutin in the prenanoemulsion. From high-performance liquid chromatography (HPLC), the amount of rutin in the prenanoemulsion was determined to be 9.27%. The cytotoxic effect of rutin in the preemulsion was investigated viain vitro tests to determine rutin’s efficacy in A549 lung cancer cell and colon cancer cell treatment. The results demonstrated that rutin in the prenanoemulsion could inhibit A549 lung cancer cells and colon cancer cells efficiently.


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## Body

## 1. Introduction

Rutin (3,3′,4′,5,7-pentahydroxyflavone-3-rhamnoglucoside) whose chemical structure is shown in Figure 1 is a flavonol, which is plentifully found in many typical plants such as passion flowers, buckwheats, black teas, and apples. It has diverse biological activities and pharmacological applications, i.e., antioxidation, antitumor, anti-inflammatory, antivirus, and low toxicity with potential clinical applications [1]. Amongst all the applications, the anticancer effect of rutin has been extensively investigated. Lin et al. reported that rutin with the dose of 120 mg/kg caused a significant reduction in the tumor size of the murine model implanted human leukemia HL-60 cells [2]. From the study on the LAN-5 neuroblastoma cell line, rutin showed the capability of decreasing levels of MYCN mRNA, the secretion of TNF-α, and reducing BLC2 expression as well as BCL2/BAX ratio [3]. Besides, rutin also showed its effect on many other cancer cell lines such as CRC colorectal cancer cells, B16F10 lung cancer cells, and HTC liver cancer cells. It was demonstrated that rutin affected the cell capture and apoptosis processes, reducing the number of metastatic nodules and cytotoxicity [4–6]. However, rutin was slightly absorbed in the gastrointestinal tract due to the poor water solubility (0.8 mg/mL) [7]. Hence, the oral bioavailability of rutin is rather low (around 20%) [8].Figure 1
The chemical structure of rutin [26].Numerous approaches have been investigated to improve the bioavailability of rutin. These approaches include the preparation of nanoparticular systems [9–13], nanophytosome [14], and cyclodextrin complexes [15, 16]. Recently, nanoparticle-based drug delivery systems have been further utilized in cancer treatment. The advantages of the nanoparticle-based drug delivery systems are that nanoparticle drugs could be designed to achieve longer circulation time, better stability, more improved internal medicine accumulations and concentrations, and better ability of reducing the toxicity to normal tissues [17]. Nanoparticle-based drug delivery systems (such as nanoemulsion, liposomes, prenanoemulsion, niosomes, and nanoparticles) are commonly stabilized with surfactants or polymers [18]. Recently, the prenanoemulsion drug delivery system has been emerging as one of the most interesting approaches to improve the poor solubility of drugs. Prenanoemulsion is a mixture composed of surfactants, cosurfactants, and drug substances, which can form a nanoemulsion under conditions of gastrointestinal fluids and gastrointestinal motilities after the oral administration. It was noted that prenanoemulsion does not contain water and thus its physical and chemical stability lasts longer than those in nanoemulsion [19]. The formation of rutin in nanoemulsion with a particle size less than 100 nm could enhance the solubility and the absorption of drugs in the gastrointestinal tract [20]. The solubility and stability of the prenanoemulsion system closely depend on screening surfactants such as Tween-80, Labrasol, Cremophor RH 40, and Kolliphor-HS15 together with other cosurfactants, i.e., Carbitol, Transcutol-P, and polyethylene glycol- (PEG-) 200, 400, and 600 [21]. The selection of the excipients for the preparation of the prenanoemulsion as anticancer drug delivery systems must ensure that the system should have long-standing stability, a long circulation time, and the ability to increase the drug accumulation in cancer cells. Another requirement is that the system should not be toxic to normal cells.Rutin is widely acknowledged for good solubility in Tween-80 and PEG-600 [21]. Tween-80 is a nonionic surfactant which is well known for its hydrophilicity and biodegradability. It exhibits no toxicity for cell at low concentration [22]. Previous works showed that Tween-80 has the ability to inhibit the mononuclear phagocyte systems and prolong the circulation time of the nanoparticles which then extend drug release times [23, 24]. PEG, on the other hand, is a polymer possessing hydrophilicity, biocompatibility, and nonallergenicity. It plays a role as a good stabilizer for the colloidal system [24, 25]. Therefore, in this study, Tween-80 and PEG-600 were selected as the excipients for the preparation of prenanoemulsion of rutin. To the best of our knowledge, there is few research on the formulation of rutin in the preemulsion system and its cytotoxicity on lung and colon cancers [21]. The aim of our study is to assess the cytotoxic effect of the prenanoemulsion system of rutin in lung cancer (A549) cells, colon cancer (Caco-2) cells, and HDF human fibroblast cells. The rutin in the prenanoemulsion system containing Tween-80 and PEG-600 was prepared with the optimal formulation of substances. The efficacy in anticancer treatments and the safety of fabricated rutin prenanoemulsion were evaluated through the MTS assay. In the in vitro test, the lung cancer (A549) cells, colon cancer (Caco-2) cells, and HDF human fibroblast cells were treated with the rutin prenanoemulsion, pure rutin, and the excipients (mixture of Tween-80 and PEG-600), respectively.


## 2. Materials and Methods

### 2.1. Materials

Rutin was provided by Tokyo Chemical Industry Co., Ltd. (APAC). Ethanol (99.8%) and polyethylene glycol with a molecular weight of 600 g/mol (PEG-600) were supplied by the NOF Corporation of Shibuya-Ku, Tokyo. Tween-80 was provided by INEOS Oxide (Lavéra France). DMEM (Gibco), CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega), and fetal bovine serum (FBS) and penicillin-streptomycin (PS) were supplied by Sigma-Aldrich. All reagents were analytical grade and used as being received without further purification.The cell lines used in thein vitro tests were included lung cancer (A549) cell lines, colon cancer (Caco-2) cell lines, and HDF human fibroblast cell lines provided by ATCC.


### 2.2. Methods

#### 2.2.1. Preparation of the Rutin Prenanoemulsion Drug Delivery System

A solution of rutin was prepared by dissolving rutin in ethanol at the concentration of 0.02 g/mL and stirring at 400 rpm for 10 minutes at room temperature. PEG-600 and Tween-80 were then added to the solution at a ratio 10%w/w and 30% w/w, respectively. The rutin prenanoemulsion system was maintained at 60°C, mixed at 400 rpm by using a magnetic stirrer for 30 minutes, and continuously dispersed using the ultrasonicator. The prenanoemulsion system was left overnight at room temperature then homogenized for 60 minutes at a normal condition. After that, the prenanoemulsion system was evaporated for 60 minutes at 50°C to remove residual ethanol. Finally, it was stored at room temperature for further analysis.


### 2.3. Characterizations

The water solubility of the rutin prenanoemulsion was tested by the Tyndall effect. Rutin preemulsion dispersion and rutin dispersion were prepared by adding the same amount of the prenanoemulsion of rutin and pure rutin into two beakers containing water. The red-wavelength laser was beamed through each dispersion, and the photographs were taken.The morphology of the rutin in the water dispersion was observed using the transmission electron microscopy (TEM) (JEOL Co., JEM-2100, Tokyo, Japan) at an acceleration voltage of 200 kV. The prenanoemulsion of rutin was dispersed in water and then dropped into a specimen. The specimen was dried in a vacuum oven before observation. The average size of the rutin nanoparticles in the suspension was also estimated.The particle size distribution of rutin prenanoemulsion was determined by a dynamic laser scattering technique (DLS) using Horiba SZ-100. The sample was diluted with water to a suitable concentration, and the measurement was carried out at a scattering angle of 90° at room temperature three times.The FTIR of rutin prenanoemulsion, pure rutin, PEG, and Tween-80 were recorded on the JASCO 4600 spectrometer in the range of 4000-400 cm-1 with a resolution of 4 cm-1. The number of scans is 64. All FTIR measurements were performed at room temperature.The NMR measurements of samples were performed with the JEOL FT-NMR 400 MHz (Japan). The samples were dissolved in methanol-d4 and subjected to the measurement with 128 scans. The chemical shift is referenced with the proton signal of methanol-d4.The amount of the rutin in the rutin prenanoemulsion was determined by the HPLC equipped with UV detected. Chromatographic conditions used the Agilent Zorbax Eclipse XDB C18 column (150×4.6 mm; 5 μm), ultraviolet spectrophotometer at 257 nm, and mobile phase: methanol-1% acetic acid solution (40 : 60, v/v). The method has a linear range of 4.97-298.47 μg/mL, limit of quantity 0.205 μg/mL, and recovery from 99.87%-102.3%.


### 2.4. Cell Viability Assay Test

The cell viability was determined to compare the cytotoxic of pure rutin and rutin prenanoemulsion in A549 lung cancer cells, Caco-2 colon cancer cells, and HDF human fibroblasts after the MTS assay. The cells were seeded in 96-well microtiter plates at a density of 5000 cells/well in Dulbecco’s modified Eagle’s medium (DMEM). The mediums were supplied with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS) and were maintained in a humidified incubator containing 5% CO2 at 37°C for 24 hours. After being seeded, the cells were treated with various pure rutin concentrations, prenanoemulsion rutin, excipients, control vehicle solution (DMSO 0.1% in phosphate buffer saline), or control solutions (culture medium without test sample and DMSO). Seventy-two hours after the treatment, 20 μL MTS was added to a culture medium, and incubation was continued at 37°C for 4 hours. The color intensity was measured at 490 nm with an ELISA microplate reader (BioTek Synergy® HT microplate reader (USA)). The experiments were performed in triplicate.A549 lung cancer cells were incubated with multiple concentrations of samples of 30, 50, 100, 150, 200, and 300μM.Caco-2 colon cancer cells were incubated at various concentrations of samples of 20, 30, 50, 100, 150, 200, and 300μM.HDF human fibroblast cells were incubated at a range of concentrations of samples from 50 to 300μM.The MTS assay is based on the conversion of MTS substrate [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] into MTS-formazan by the dehydrogenase respiratory enzyme in the mitochondria of living cells. The MTS-formazan solution that dissolves in the culture medium has a blue-violet color and maximizes the absorption at 490 nm. The number of living cells is directly proportional to the formazan concentration, expressed in the solution’s optical density value at 490 nm.The percentage of living cells determined from OD490 values was obtained using the following equation:(1)Thepercentageoflivingcells%=OD490sample−OD490blankOD490control−OD490blank×100%.


### 2.5. Statistical Analysis

All the results were presented as ±standard error of the mean value. Statistical analysis was performed using ANOVA. A value ofp<0.05 was considered statistically significant.


## 2.1. Materials

Rutin was provided by Tokyo Chemical Industry Co., Ltd. (APAC). Ethanol (99.8%) and polyethylene glycol with a molecular weight of 600 g/mol (PEG-600) were supplied by the NOF Corporation of Shibuya-Ku, Tokyo. Tween-80 was provided by INEOS Oxide (Lavéra France). DMEM (Gibco), CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega), and fetal bovine serum (FBS) and penicillin-streptomycin (PS) were supplied by Sigma-Aldrich. All reagents were analytical grade and used as being received without further purification.The cell lines used in thein vitro tests were included lung cancer (A549) cell lines, colon cancer (Caco-2) cell lines, and HDF human fibroblast cell lines provided by ATCC.


## 2.2. Methods

### 2.2.1. Preparation of the Rutin Prenanoemulsion Drug Delivery System

A solution of rutin was prepared by dissolving rutin in ethanol at the concentration of 0.02 g/mL and stirring at 400 rpm for 10 minutes at room temperature. PEG-600 and Tween-80 were then added to the solution at a ratio 10%w/w and 30% w/w, respectively. The rutin prenanoemulsion system was maintained at 60°C, mixed at 400 rpm by using a magnetic stirrer for 30 minutes, and continuously dispersed using the ultrasonicator. The prenanoemulsion system was left overnight at room temperature then homogenized for 60 minutes at a normal condition. After that, the prenanoemulsion system was evaporated for 60 minutes at 50°C to remove residual ethanol. Finally, it was stored at room temperature for further analysis.


## 2.2.1. Preparation of the Rutin Prenanoemulsion Drug Delivery System

A solution of rutin was prepared by dissolving rutin in ethanol at the concentration of 0.02 g/mL and stirring at 400 rpm for 10 minutes at room temperature. PEG-600 and Tween-80 were then added to the solution at a ratio 10%w/w and 30% w/w, respectively. The rutin prenanoemulsion system was maintained at 60°C, mixed at 400 rpm by using a magnetic stirrer for 30 minutes, and continuously dispersed using the ultrasonicator. The prenanoemulsion system was left overnight at room temperature then homogenized for 60 minutes at a normal condition. After that, the prenanoemulsion system was evaporated for 60 minutes at 50°C to remove residual ethanol. Finally, it was stored at room temperature for further analysis.


## 2.3. Characterizations

The water solubility of the rutin prenanoemulsion was tested by the Tyndall effect. Rutin preemulsion dispersion and rutin dispersion were prepared by adding the same amount of the prenanoemulsion of rutin and pure rutin into two beakers containing water. The red-wavelength laser was beamed through each dispersion, and the photographs were taken.The morphology of the rutin in the water dispersion was observed using the transmission electron microscopy (TEM) (JEOL Co., JEM-2100, Tokyo, Japan) at an acceleration voltage of 200 kV. The prenanoemulsion of rutin was dispersed in water and then dropped into a specimen. The specimen was dried in a vacuum oven before observation. The average size of the rutin nanoparticles in the suspension was also estimated.The particle size distribution of rutin prenanoemulsion was determined by a dynamic laser scattering technique (DLS) using Horiba SZ-100. The sample was diluted with water to a suitable concentration, and the measurement was carried out at a scattering angle of 90° at room temperature three times.The FTIR of rutin prenanoemulsion, pure rutin, PEG, and Tween-80 were recorded on the JASCO 4600 spectrometer in the range of 4000-400 cm-1 with a resolution of 4 cm-1. The number of scans is 64. All FTIR measurements were performed at room temperature.The NMR measurements of samples were performed with the JEOL FT-NMR 400 MHz (Japan). The samples were dissolved in methanol-d4 and subjected to the measurement with 128 scans. The chemical shift is referenced with the proton signal of methanol-d4.The amount of the rutin in the rutin prenanoemulsion was determined by the HPLC equipped with UV detected. Chromatographic conditions used the Agilent Zorbax Eclipse XDB C18 column (150×4.6 mm; 5 μm), ultraviolet spectrophotometer at 257 nm, and mobile phase: methanol-1% acetic acid solution (40 : 60, v/v). The method has a linear range of 4.97-298.47 μg/mL, limit of quantity 0.205 μg/mL, and recovery from 99.87%-102.3%.


## 2.4. Cell Viability Assay Test

The cell viability was determined to compare the cytotoxic of pure rutin and rutin prenanoemulsion in A549 lung cancer cells, Caco-2 colon cancer cells, and HDF human fibroblasts after the MTS assay. The cells were seeded in 96-well microtiter plates at a density of 5000 cells/well in Dulbecco’s modified Eagle’s medium (DMEM). The mediums were supplied with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS) and were maintained in a humidified incubator containing 5% CO2 at 37°C for 24 hours. After being seeded, the cells were treated with various pure rutin concentrations, prenanoemulsion rutin, excipients, control vehicle solution (DMSO 0.1% in phosphate buffer saline), or control solutions (culture medium without test sample and DMSO). Seventy-two hours after the treatment, 20 μL MTS was added to a culture medium, and incubation was continued at 37°C for 4 hours. The color intensity was measured at 490 nm with an ELISA microplate reader (BioTek Synergy® HT microplate reader (USA)). The experiments were performed in triplicate.A549 lung cancer cells were incubated with multiple concentrations of samples of 30, 50, 100, 150, 200, and 300μM.Caco-2 colon cancer cells were incubated at various concentrations of samples of 20, 30, 50, 100, 150, 200, and 300μM.HDF human fibroblast cells were incubated at a range of concentrations of samples from 50 to 300μM.The MTS assay is based on the conversion of MTS substrate [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] into MTS-formazan by the dehydrogenase respiratory enzyme in the mitochondria of living cells. The MTS-formazan solution that dissolves in the culture medium has a blue-violet color and maximizes the absorption at 490 nm. The number of living cells is directly proportional to the formazan concentration, expressed in the solution’s optical density value at 490 nm.The percentage of living cells determined from OD490 values was obtained using the following equation:(1)Thepercentageoflivingcells%=OD490sample−OD490blankOD490control−OD490blank×100%.


## 2.5. Statistical Analysis

All the results were presented as ±standard error of the mean value. Statistical analysis was performed using ANOVA. A value ofp<0.05 was considered statistically significant.


## 3. Results and Discussion

### 3.1. Characterizations

The screening formulation of the prenanoemulsion system was usually considered involving the following factors. First, the formulation composition should be simple, safe, and compatible. Second, the formulation should have good solubility and effective droplet size after forming a microemulsion [27, 28]. The selection of the surfactant/cosurfactant mixtures was primarily considered for the homogeneity of the system. This is much related to the Hydrophilic-Lipophilic Balance (HLB) of the surfactant. The surfactants with HLB of 12-15 are good choices for the best efficiency. Tween-80 with HLB of 15 combined with PEG-600 as cosurfactant can adjust the HLB of surfactant and increase the stability of microemulsion and enhance the solubility of hydrophobic drug [29]. Therefore, the excipients, Tween-80 and PEG-600, were chosen to load rutin in the preemulsion system. Several formulations were performed to evaluate the formation of prenanoemulsion of rutin. The best results were obtained with PEG-600 and Tween-80 at a concentration of 10% w/w and 30% w/w, respectively. We used this formulation of rutin preemulsion for further studies.The solubility of prenanoemulsion of rutin in water is assessed indirectly through dispersing of preemulsion into the water and observed the light scattered through the dispersion, that is, the Tyndall effect.The Tyndall effect is a phenomenon which is based on the light scattering caused by particles in colloidal or suspension. This effect is employed to verify scattering ray caused by particles in the colloidal or suspension and assess the homogeneity of the solution from the scattering [30]. When the beam of light with a wavelength in the range of 630-680 nm went through liquid-containing particles with the size from 40 to 900 nm, these particles scatter the ray; thus, they become brighter spots which can be observed [30]. The image of the Tyndall effect of the two dispersions containing pure rutin and rutin in the prenanoemulsion is presented in Figure 2. The observed results indicated that rutin in the preemulsion with PEG/Tween was well dispersed in water.Figure 2
The Tyndall effect of (a) rutin in water, (b) prenanoemulsion of rutin in water, and (c) pure water.
(a)(b)(c)The morphology of rutin particle in the prenanoemulsion is investigated and shown in Figure3. The rutin nanoparticles had a spherical shape with a smooth surface. The rutin particles were uniform in size and shape and distributed evenly in the water emulsion. The average size of the rutin nanoparticles was about 15 nm. Previous studies [31–33] have shown that nanoparticles with size ranging from 10 to 100 nm are suitable for drug delivery in the body. Nanoparticles with a size larger than 10 nm will not be leaked into capillaries and will then be removed by single-pass renal clearance. Nanoparticles with size less than 100 nm can escape from being captured by the macrophage in the mononuclear phagocyte system and easily penetrate into the human organs, especially tumors. This is because the size of blood vessels of tumors ranges from 100 to 600 nm. Therefore, the size of nanoparticles less than 100 nm is optimal for the accumulation of the toxic compounds in the cancer cells [31–33]. Another reason is that the spherical-shaped nanoparticles have better mobility in the blood vessel than nanoparticles with other shapes. Thus, the prenanoemulsion of rutin as a drug delivery system was proved to be successfully fabricated. The dispersion of the prenanoemulsion system in water has generated rutin nanoparticles with the desired shapes and sizes targeted for enhancing its bioactivity.Figure 3
The HR-TEM image of nanorutin after dissolving the preemulsion in water.The size distribution profile of rutin nanoemulsion is illustrated in Figure4. It was seen that the distribution of rutin particle is unimodal with narrow distribution having a polydisperse index of 1.5. This suggested that the rutin particles are quite uniform with an average size of 8-15 nm. The size of rutin nanoparticles measured from DLS analysis was similar to that estimated from TEM.Figure 4
Particle size distribution profile of rutin emulsion in water.The chemical structure of rutin and its interactions with a mixture of PEG/Tween in the prenanoemulsion with PEG/Tween was investigated by FTIR spectroscopy. Figure5 shows the FTIR of pure rutin, rutin prenanoemulsion, PEG, and Tween. In the IR spectra of pure rutin, the absorption band at 3402 cm-1 was assigned to OH groups and =C-H bonds in the benzene ring. The absorption band at 1637 cm-1 was assigned to the vibration of the C=O bond. Additionally, the absorption band due to the C=C bond at 1601 cm-1 verified the presence of the aromatic alkene group. In the IR spectrum of rutin prenanoemulsion, the absorption band at 3424 cm-1 was ascribed for OH groups and aromatic C-H linkages. The absorption band appeared at 2980 cm-1 belonged to the aliphatic C-H bond in Tween and PEG. The characteristic absorption band of rutin prenanoemulsion was similar to those of PEG, and Tween and most of the characteristic bands corresponding to rutin did not appear. However, the carbonyl absorption band was shifted from 1637 cm-1 in the FTIR spectrum of rutin to 1644 cm-1 in the FTIR spectrum of rutin prenanoemulsion. The shift in the absorption band may be due to the interactions involving hydrogen bonding between rutin and PEG/Tween mixture. The interactions probably favour the hydrophilic characteristics of rutin in the prenanoemulsion.Figure 5
FTIR spectrum of rutin, PEG, Tween, and prenanoemulsion rutin.Figure6 shows the 1H-NMR spectrum of rutin prenanoemulsion. In the spectrum, the signal at 4.4 ppm is assigned to the proton of methanol-d4. The group of signals at 3.6-3.7 ppm is the characteristic peaks of PEG. The signals marked with asterisk were assigned to Tween-80. To assign thoroughly the signals of rutin, the rutin prenanoemulsion was acidified with HCl 1 M to remove substantial amount of PEG and Tween. The sedimental was collected and subjected to NMR measurement.Figure 6
1H-NMR spectrum of rutin prenanoemulsion (400 MHz, in methanol-d4).Figure7 presents the 1H-NMR spectra of pure rutin and rutin prenanoemulsion after the acidification. It could be seen that after the acidification, most of Tween and PEG were removed, corresponding to the significant decrease in the signal intensity of Tween and PEG. Consequently, the small signals of rutin were observed. The assignment for the signals is given in Figure 7. From the assignments, it confirmed that the chemical structure of rutin was almost preserved in the prenanoemulsion system. Therefore, we could conclude that the formation of prenanoemulsion with the PEG/Tween system did not change the chemical characteristics of rutin.Figure 7
1H-NMR of (a) rutin and (b) rutin prenanoemulsion after acidification (400 MHz, in methanol-d4).


### 3.2.In Vitro Cytotoxicity of the Rutin Prenanoemulsion System

From the TEM result, the rutin nanoparticle was liberated into the water phase with the size of 15 nm. The efficacy of this drug delivery system could be enhanced due to the small size of active compounds of rutin. The anticancer effects and the safety of rutin prenanoemulsion were investigated throughin vitro assay tests. In the in vitro assay, lung cancer cells, colon cancer cells, and human fibroblast cells were treated with various concentrations of pure rutin, rutin prenanoemulsion, and the excipients for 72 hours. The rutin concentration in the prenanoemulsion rutin sample was determined by HPLC with the UV detector. The analysis showed that the amount of rutin in the prenanoemulsion is about 9.27%. From this concentration, the rutin prenanoemulsion sample was diluted to various rutin nanoemulsions with the concentration ranges from 30 to 300 μM for the MTS assay.Figure8 shows the MTS assay test results when lung cancer cells A549 were incubated with pure rutin, rutin nanoemulsion, and the excipients at the concentrations ranging from 30 to 300 μM for 72 hours. The reduction in the MTS test indicates the viability and the proliferation of the cells. The results in Figure 8 showed that the living rate of A549 lung cancer cell was remarkably different between the groups of wells supplemented with different reagents (control, pure rutin, rutin nanoemulsion, and excipients) and at different concentrations (∗p<0.05 and #p<0.05). The viability of A549 lung cancer cells decreased as concentrations of nanoemulsion rutin increased in the range of 100-300 μM. At the same concentration of 150 μM, rutin nanoemulsion, pure rutin, and the excipients caused 44.04%, 4.65%, and 5.35% of cell death, respectively. At the concentration of 300 μM, the rutin nanoemulsion, pure rutin, and excipients, the rate of cell death in the wells was 99.74%, 4.4%, and 16.48%, respectively. These results revealed that at the concentration range of 150-300 μM, the rutin prenanoemulsion caused higher viability in inhibition and proliferation of the A549 lung cancer cells than pure rutin and the excipients.Figure 8
Cytotoxicity effects of pure rutin, prenanoemulsion of rutin, and excipients (PEG and Tween 80) to A549 lung cancer cells.The IC50 values of pure rutin, rutin nanoemulsion, and the excipients were determined by the rate of survival of A549 lung cancer cells after they were all treated with the studied concentrations. From the MTS assay results, the typical equation describing the correlation between the rate of survival of A549 cells and the corresponding concentration of nanorutin was established as follows:(2)y=−83.56lnx+471.32R2=0.9654,in which, x represents the concentration of nanorutin (μM) and y represents the percentage of viable cells of sample wells compared to control wells without samples.From Equation (2), when y value was taken as 50, the IC50 values of nanoemulsion rutin in A549 lung cancer cell line were determined to be 154.8 μM. On the other hand, Figure 8 shows that the IC50 values of pure rutin and the excipients were higher than 300 μM. These results indicated that rutin prenanoemulsion can inhibit A549 lung cancer cells from concentrations higher than 150 μM; pure rutin and the excipients are nontoxic to those cells at concentrations lower than 300 μM. In the study reported by Goitia et al., the cytotoxicity of the complex (Na2[VO(rutin)(OH)2]·5H2O) in A549 lung cancer cells has the IC50 value of 93 μM [34]. The IC50 value of the complex was lower than the IC50 value of the rutin prenanoemulsion in this study (150 μM). These results indicate that the encapsulation of rutin in PEG/Tween plays an important role in the enhancement of cytotoxic activity. It may be explained that rutin nanoemulsion is well soluble in water. The increased hydrophilic moiety of rutin molecules in the nanoemulsion decreases the energy barrier and subsequently increases the intermembrane transfer rate by over 25-fold [35]. Therefore, the absorption of rutin in PEG into cells is higher than this of pure rutin.To evaluate the cytotoxicity of the rutin prenanoemulsion in the Caco2 colon cancer cells, the assay was prepared in the same way as for the lung cancer cells. These results are shown in Figure9.Figure 9
Cytotoxicity effects of pure rutin, rutin prenanoemulsion, and the excipients (PEG and Tween-80) to Caco2 colon cancer cells.Figure9 shows the results of the MTS test when Caco2 colon cancer cells were incubated with pure rutin, rutin prenanoemulsion, and the excipients at concentrations in the range of 30-300 μM for 72 hours. As similar as the results observed for A549 lung cancer cell, the living rate of Caco2 colon cancer cell was drastically different between the groups of wells supplemented with different reagents (control, pure rutin, rutin prenanoemulsion, and the excipients) and at different concentrations (∗p<0.05 and #p<0.05). The rutin prenanoemulsion had a cytotoxic effect on the viability and proliferation of Caco2 colon cancer cells within the concentration range of 150-500 μM. However, pure rutin and the excipients exhibited no cytotoxic effect in Caco2 cells at the concentration of less than 500 μM. At the concentrations of 500 μM, the rate of cell death in those wells containing rutin prenanoemulsion, pure rutin, or the excipients compared to the control wells was 82.17%, 25.45%, and 36.75%, respectively. From the results of the MTS assay, the general equation describing the correlation between the rate of survival of Caco2 cells and the corresponding concentration of nanorutin was shown as follows:
(3)y=−44.93lnx+298.33R2=0.9949.From Equation (3), the IC50 value of rutin prenanoemulsion in the Caco2 cell line was 251.5 μM. The IC50 value of pure rutin and the excipients was higher than 500 μM. The result demonstrated that the cytotoxic effect of pure rutin and rutin prenanoemulsion depends on the different cell lines (shown in Figure 10). ben Sghaier et al. also reported the similar results of IC50, in which the IC50 values of pure rutin in A549 lung and Caco2 cancer cells were 559.83 and 710.59 μM, respectively [36]. These results indicated that pure rutin and rutin prenanoemulsion had higher cytotoxicity to A549 cells than Caco2 cells. Rutin prenanoemulsion is more effective in treating anticancer than pure rutin. It is probably because that rutin prenanoemulsion dissolves in culture medium better than pure rutin. Moreover, with nanosize of rutin particles, i.e., 15 nm, rutin in the prenanoemulsion was easily penetrated through cell membranes. Previous studies have demonstrated that nanoparticles with size ranging from 10 to 100 nm are ideal for the drug delivery system to intracellular internalization [37–39]. The bioavailability of rutin in preemulsion with the PEG/Tween mixture has been significantly enhanced. This is because the short-chain length of PEG causes a higher probability of nonspecific protein absorption resulting in a higher uptake of cancer cells [40] and Tween-80 could help to increase the permeability through the cell membrane [41].Figure 10
Comparing the inhibitory effect of rutin prenanoemulsion in cancer cell lines and healthy cell lines.The MTS assay of rutin prenanoemulsion and the PEG/Tween mixture in healthy human fibroblast cell line was conducted to assess the safety of the rutin prenanoemulsion. Healthy human fibroblast cells were incubated with prenanoemulsion rutin, the mixture PEG/Tween, control vehicle solution, or control solution at a concentration in the range 30-300μM for 72 hours. Figure 11 shows the cytotoxicity effects of rutin prenanoemulsion and the excipients to healthy human fibroblast cells. It showed that rutin prenanoemulsion and the PEG/Tween mixture had no cytotoxic effects on fiber cells at the concentrations less than 300 μM. The results revealed that in the concentration range of 100-300 μM, the rutin prenanoemulsion inhibited viability and proliferation of the cancer cells but showed the safety for normal cells.Figure 11
Cytotoxicity effects of rutin prenanoemulsion and the excipients (PEG-600 and Tween-80) to healthy human fibroblast cells.


## 3.1. Characterizations

The screening formulation of the prenanoemulsion system was usually considered involving the following factors. First, the formulation composition should be simple, safe, and compatible. Second, the formulation should have good solubility and effective droplet size after forming a microemulsion [27, 28]. The selection of the surfactant/cosurfactant mixtures was primarily considered for the homogeneity of the system. This is much related to the Hydrophilic-Lipophilic Balance (HLB) of the surfactant. The surfactants with HLB of 12-15 are good choices for the best efficiency. Tween-80 with HLB of 15 combined with PEG-600 as cosurfactant can adjust the HLB of surfactant and increase the stability of microemulsion and enhance the solubility of hydrophobic drug [29]. Therefore, the excipients, Tween-80 and PEG-600, were chosen to load rutin in the preemulsion system. Several formulations were performed to evaluate the formation of prenanoemulsion of rutin. The best results were obtained with PEG-600 and Tween-80 at a concentration of 10% w/w and 30% w/w, respectively. We used this formulation of rutin preemulsion for further studies.The solubility of prenanoemulsion of rutin in water is assessed indirectly through dispersing of preemulsion into the water and observed the light scattered through the dispersion, that is, the Tyndall effect.The Tyndall effect is a phenomenon which is based on the light scattering caused by particles in colloidal or suspension. This effect is employed to verify scattering ray caused by particles in the colloidal or suspension and assess the homogeneity of the solution from the scattering [30]. When the beam of light with a wavelength in the range of 630-680 nm went through liquid-containing particles with the size from 40 to 900 nm, these particles scatter the ray; thus, they become brighter spots which can be observed [30]. The image of the Tyndall effect of the two dispersions containing pure rutin and rutin in the prenanoemulsion is presented in Figure 2. The observed results indicated that rutin in the preemulsion with PEG/Tween was well dispersed in water.Figure 2
The Tyndall effect of (a) rutin in water, (b) prenanoemulsion of rutin in water, and (c) pure water.
(a)(b)(c)The morphology of rutin particle in the prenanoemulsion is investigated and shown in Figure3. The rutin nanoparticles had a spherical shape with a smooth surface. The rutin particles were uniform in size and shape and distributed evenly in the water emulsion. The average size of the rutin nanoparticles was about 15 nm. Previous studies [31–33] have shown that nanoparticles with size ranging from 10 to 100 nm are suitable for drug delivery in the body. Nanoparticles with a size larger than 10 nm will not be leaked into capillaries and will then be removed by single-pass renal clearance. Nanoparticles with size less than 100 nm can escape from being captured by the macrophage in the mononuclear phagocyte system and easily penetrate into the human organs, especially tumors. This is because the size of blood vessels of tumors ranges from 100 to 600 nm. Therefore, the size of nanoparticles less than 100 nm is optimal for the accumulation of the toxic compounds in the cancer cells [31–33]. Another reason is that the spherical-shaped nanoparticles have better mobility in the blood vessel than nanoparticles with other shapes. Thus, the prenanoemulsion of rutin as a drug delivery system was proved to be successfully fabricated. The dispersion of the prenanoemulsion system in water has generated rutin nanoparticles with the desired shapes and sizes targeted for enhancing its bioactivity.Figure 3
The HR-TEM image of nanorutin after dissolving the preemulsion in water.The size distribution profile of rutin nanoemulsion is illustrated in Figure4. It was seen that the distribution of rutin particle is unimodal with narrow distribution having a polydisperse index of 1.5. This suggested that the rutin particles are quite uniform with an average size of 8-15 nm. The size of rutin nanoparticles measured from DLS analysis was similar to that estimated from TEM.Figure 4
Particle size distribution profile of rutin emulsion in water.The chemical structure of rutin and its interactions with a mixture of PEG/Tween in the prenanoemulsion with PEG/Tween was investigated by FTIR spectroscopy. Figure5 shows the FTIR of pure rutin, rutin prenanoemulsion, PEG, and Tween. In the IR spectra of pure rutin, the absorption band at 3402 cm-1 was assigned to OH groups and =C-H bonds in the benzene ring. The absorption band at 1637 cm-1 was assigned to the vibration of the C=O bond. Additionally, the absorption band due to the C=C bond at 1601 cm-1 verified the presence of the aromatic alkene group. In the IR spectrum of rutin prenanoemulsion, the absorption band at 3424 cm-1 was ascribed for OH groups and aromatic C-H linkages. The absorption band appeared at 2980 cm-1 belonged to the aliphatic C-H bond in Tween and PEG. The characteristic absorption band of rutin prenanoemulsion was similar to those of PEG, and Tween and most of the characteristic bands corresponding to rutin did not appear. However, the carbonyl absorption band was shifted from 1637 cm-1 in the FTIR spectrum of rutin to 1644 cm-1 in the FTIR spectrum of rutin prenanoemulsion. The shift in the absorption band may be due to the interactions involving hydrogen bonding between rutin and PEG/Tween mixture. The interactions probably favour the hydrophilic characteristics of rutin in the prenanoemulsion.Figure 5
FTIR spectrum of rutin, PEG, Tween, and prenanoemulsion rutin.Figure6 shows the 1H-NMR spectrum of rutin prenanoemulsion. In the spectrum, the signal at 4.4 ppm is assigned to the proton of methanol-d4. The group of signals at 3.6-3.7 ppm is the characteristic peaks of PEG. The signals marked with asterisk were assigned to Tween-80. To assign thoroughly the signals of rutin, the rutin prenanoemulsion was acidified with HCl 1 M to remove substantial amount of PEG and Tween. The sedimental was collected and subjected to NMR measurement.Figure 6
1H-NMR spectrum of rutin prenanoemulsion (400 MHz, in methanol-d4).Figure7 presents the 1H-NMR spectra of pure rutin and rutin prenanoemulsion after the acidification. It could be seen that after the acidification, most of Tween and PEG were removed, corresponding to the significant decrease in the signal intensity of Tween and PEG. Consequently, the small signals of rutin were observed. The assignment for the signals is given in Figure 7. From the assignments, it confirmed that the chemical structure of rutin was almost preserved in the prenanoemulsion system. Therefore, we could conclude that the formation of prenanoemulsion with the PEG/Tween system did not change the chemical characteristics of rutin.Figure 7
1H-NMR of (a) rutin and (b) rutin prenanoemulsion after acidification (400 MHz, in methanol-d4).


## 3.2.In Vitro Cytotoxicity of the Rutin Prenanoemulsion System

From the TEM result, the rutin nanoparticle was liberated into the water phase with the size of 15 nm. The efficacy of this drug delivery system could be enhanced due to the small size of active compounds of rutin. The anticancer effects and the safety of rutin prenanoemulsion were investigated throughin vitro assay tests. In the in vitro assay, lung cancer cells, colon cancer cells, and human fibroblast cells were treated with various concentrations of pure rutin, rutin prenanoemulsion, and the excipients for 72 hours. The rutin concentration in the prenanoemulsion rutin sample was determined by HPLC with the UV detector. The analysis showed that the amount of rutin in the prenanoemulsion is about 9.27%. From this concentration, the rutin prenanoemulsion sample was diluted to various rutin nanoemulsions with the concentration ranges from 30 to 300 μM for the MTS assay.Figure8 shows the MTS assay test results when lung cancer cells A549 were incubated with pure rutin, rutin nanoemulsion, and the excipients at the concentrations ranging from 30 to 300 μM for 72 hours. The reduction in the MTS test indicates the viability and the proliferation of the cells. The results in Figure 8 showed that the living rate of A549 lung cancer cell was remarkably different between the groups of wells supplemented with different reagents (control, pure rutin, rutin nanoemulsion, and excipients) and at different concentrations (∗p<0.05 and #p<0.05). The viability of A549 lung cancer cells decreased as concentrations of nanoemulsion rutin increased in the range of 100-300 μM. At the same concentration of 150 μM, rutin nanoemulsion, pure rutin, and the excipients caused 44.04%, 4.65%, and 5.35% of cell death, respectively. At the concentration of 300 μM, the rutin nanoemulsion, pure rutin, and excipients, the rate of cell death in the wells was 99.74%, 4.4%, and 16.48%, respectively. These results revealed that at the concentration range of 150-300 μM, the rutin prenanoemulsion caused higher viability in inhibition and proliferation of the A549 lung cancer cells than pure rutin and the excipients.Figure 8
Cytotoxicity effects of pure rutin, prenanoemulsion of rutin, and excipients (PEG and Tween 80) to A549 lung cancer cells.The IC50 values of pure rutin, rutin nanoemulsion, and the excipients were determined by the rate of survival of A549 lung cancer cells after they were all treated with the studied concentrations. From the MTS assay results, the typical equation describing the correlation between the rate of survival of A549 cells and the corresponding concentration of nanorutin was established as follows:(2)y=−83.56lnx+471.32R2=0.9654,in which, x represents the concentration of nanorutin (μM) and y represents the percentage of viable cells of sample wells compared to control wells without samples.From Equation (2), when y value was taken as 50, the IC50 values of nanoemulsion rutin in A549 lung cancer cell line were determined to be 154.8 μM. On the other hand, Figure 8 shows that the IC50 values of pure rutin and the excipients were higher than 300 μM. These results indicated that rutin prenanoemulsion can inhibit A549 lung cancer cells from concentrations higher than 150 μM; pure rutin and the excipients are nontoxic to those cells at concentrations lower than 300 μM. In the study reported by Goitia et al., the cytotoxicity of the complex (Na2[VO(rutin)(OH)2]·5H2O) in A549 lung cancer cells has the IC50 value of 93 μM [34]. The IC50 value of the complex was lower than the IC50 value of the rutin prenanoemulsion in this study (150 μM). These results indicate that the encapsulation of rutin in PEG/Tween plays an important role in the enhancement of cytotoxic activity. It may be explained that rutin nanoemulsion is well soluble in water. The increased hydrophilic moiety of rutin molecules in the nanoemulsion decreases the energy barrier and subsequently increases the intermembrane transfer rate by over 25-fold [35]. Therefore, the absorption of rutin in PEG into cells is higher than this of pure rutin.To evaluate the cytotoxicity of the rutin prenanoemulsion in the Caco2 colon cancer cells, the assay was prepared in the same way as for the lung cancer cells. These results are shown in Figure9.Figure 9
Cytotoxicity effects of pure rutin, rutin prenanoemulsion, and the excipients (PEG and Tween-80) to Caco2 colon cancer cells.Figure9 shows the results of the MTS test when Caco2 colon cancer cells were incubated with pure rutin, rutin prenanoemulsion, and the excipients at concentrations in the range of 30-300 μM for 72 hours. As similar as the results observed for A549 lung cancer cell, the living rate of Caco2 colon cancer cell was drastically different between the groups of wells supplemented with different reagents (control, pure rutin, rutin prenanoemulsion, and the excipients) and at different concentrations (∗p<0.05 and #p<0.05). The rutin prenanoemulsion had a cytotoxic effect on the viability and proliferation of Caco2 colon cancer cells within the concentration range of 150-500 μM. However, pure rutin and the excipients exhibited no cytotoxic effect in Caco2 cells at the concentration of less than 500 μM. At the concentrations of 500 μM, the rate of cell death in those wells containing rutin prenanoemulsion, pure rutin, or the excipients compared to the control wells was 82.17%, 25.45%, and 36.75%, respectively. From the results of the MTS assay, the general equation describing the correlation between the rate of survival of Caco2 cells and the corresponding concentration of nanorutin was shown as follows:
(3)y=−44.93lnx+298.33R2=0.9949.From Equation (3), the IC50 value of rutin prenanoemulsion in the Caco2 cell line was 251.5 μM. The IC50 value of pure rutin and the excipients was higher than 500 μM. The result demonstrated that the cytotoxic effect of pure rutin and rutin prenanoemulsion depends on the different cell lines (shown in Figure 10). ben Sghaier et al. also reported the similar results of IC50, in which the IC50 values of pure rutin in A549 lung and Caco2 cancer cells were 559.83 and 710.59 μM, respectively [36]. These results indicated that pure rutin and rutin prenanoemulsion had higher cytotoxicity to A549 cells than Caco2 cells. Rutin prenanoemulsion is more effective in treating anticancer than pure rutin. It is probably because that rutin prenanoemulsion dissolves in culture medium better than pure rutin. Moreover, with nanosize of rutin particles, i.e., 15 nm, rutin in the prenanoemulsion was easily penetrated through cell membranes. Previous studies have demonstrated that nanoparticles with size ranging from 10 to 100 nm are ideal for the drug delivery system to intracellular internalization [37–39]. The bioavailability of rutin in preemulsion with the PEG/Tween mixture has been significantly enhanced. This is because the short-chain length of PEG causes a higher probability of nonspecific protein absorption resulting in a higher uptake of cancer cells [40] and Tween-80 could help to increase the permeability through the cell membrane [41].Figure 10
Comparing the inhibitory effect of rutin prenanoemulsion in cancer cell lines and healthy cell lines.The MTS assay of rutin prenanoemulsion and the PEG/Tween mixture in healthy human fibroblast cell line was conducted to assess the safety of the rutin prenanoemulsion. Healthy human fibroblast cells were incubated with prenanoemulsion rutin, the mixture PEG/Tween, control vehicle solution, or control solution at a concentration in the range 30-300μM for 72 hours. Figure 11 shows the cytotoxicity effects of rutin prenanoemulsion and the excipients to healthy human fibroblast cells. It showed that rutin prenanoemulsion and the PEG/Tween mixture had no cytotoxic effects on fiber cells at the concentrations less than 300 μM. The results revealed that in the concentration range of 100-300 μM, the rutin prenanoemulsion inhibited viability and proliferation of the cancer cells but showed the safety for normal cells.Figure 11
Cytotoxicity effects of rutin prenanoemulsion and the excipients (PEG-600 and Tween-80) to healthy human fibroblast cells.


## 4. Conclusions

The preparation of rutin prenanoemulsion was successfully made in this work. Rutin in the prenanoemulsion had a spherical shape with a size of 15 nm. The rutin prenanoemulsion showed good solubility in water. The chemical structure of rutin was unmodified in the prenanoemulsion system with PEG/Tween and subsequently preserved its bioactivities. When rutin was formed in the prenanoemulsion system with a small size, the rutin’s bioactivity was enhanced double. The IC50 of rutin in prenanoemulsion was determined to be 154.8μM for A549 cancer cell treatment and 251.5 μM for Caco-2 cancer cell treatment. It reveals that the formation of the rutin prenanoemulsion has potential to become a new formulation which stabilizes the rutin and increases its bioactivity towards cancer diseases.



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