# Comparative Analysis of the Full-Length Genome Sequence of a Clinical Isolate ofHuman Parainfluenza Virus 4B

Authors: John A. Lednicky; Thomas B. Waltzek; Micah D. Halpern; Sara B. Hamilton
Journal: Scientifica
 (2012)

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## Abstract

We are engaged in airborne transmission and epidemiology studies of respiratory pathogens, with particular interest inhuman parainfluenza virus type 4 (hPIV-4) and other lesser studied viruses. In this paper, hPIV-4 was detected in primary rhesus monkey kidney (PRMK) cells that had been inoculated with nasopharyngeal swab material obtained from a child with a mild upper respiratory tract illness.  Attempts to isolate the virus in pure culture were hampered by the presence of a fast-growing simian spumavirus that was a contaminant of the PRMK cells.  Total RNA was extracted from the PRMK cell culture, and PCR followed by sequencing of a subgenomic section of the fusion protein gene suggested the hPIV-4 was subtype 4B. At the time of this work, two complete but dissimilar hPIV-4B genomes had been deposited by others in GenBank. To gain better insights on hPIV-4B, and to test methods that we are developing for viral forensics, the entire genomic sequence of our virus was determined from archived RNA. The hPIV-4B genomic sequence that we determined conforms to the paramyxovirus “rule of six.” Here, we compare and contrast the genetic features of the three completely sequenced hPIV-4B genomes currently present in GenBank.


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*Source: 871201-2012-07-19.xml*