# Chitosan-Grafted Copolymers and Chitosan-Ligand Conjugates as Matrices for Pulmonary Drug Delivery

Authors: Fernanda Andrade; Francisco Goycoolea; Diego A. Chiappetta; José das Neves; Alejandro Sosnik; Bruno Sarmento
Journal: International Journal of Carbohydrate Chemistry
 (2011)

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## Abstract

Recently, much attention has been given to pulmonary drug delivery by means of nanosized systems to treat both local and systemic diseases. Among the different materials used for the production of nanocarriers, chitosan enjoys  high popularity due to its inherent characteristics such as biocompatibility, biodegradability, and mucoadhesion, among others. Through the modification of chitosan chemical structure, either by the addition of new chemical groups or by the functionalization with ligands, it is possible to obtain derivatives with advantageous and specific characteristics for pulmonary administration. In this paper, we discuss the advantages of using chitosan for nanotechnology-based pulmonary delivery of drugs and summarize the most recent and promising modifications performed to the chitosan molecule in order to improve its characteristics.


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## Body

## 1. Introduction

Alongside the successful market launch of different products over the last decades, a continuous effort to formulate delivery systems for the pulmonary administration of a wide variety of drugs has been extensively described in the literature    [1, 2]. The particular anatomical, physiological, and pathophysiological features of the respiratory tract pose enormous challenges that need to be overcome in order to obtain effective lung deposition, uniform distribution, in loco retention (main challenge is to circumvent mucocilliary clearance), and stability (particularly to enzymatic degradation) of therapeutic agents    [1, 3].Nevertheless, particular attention has been dedicated not only to drugs themselves but also to excipients required to improve the bioavailability of drugs administered pulmonarily. In this context, excipients that could transiently enhance the absorption of drugs are on the spot. Chitosan, a polysaccharide with structural characteristics similar to glucosamines and obtained by the alkaline deacetylation of chitin, derived from the exoskeleton of crustaceans, is one of such appealing excipients.The safety and tolerability of chitosan are synergistic characteristics towards its application in drug delivery by different administration routes. Despite the natural properties, some drawbacks are associated with the poor solubility at physiologic pH and the passive targeting effect. Thus, chemical modifications of chitosan by conjugating various functional groups allow the control of the hydrophilicity and the solubility at neutral and basic pH and open new opportunities to expand the application of this biopolymer.In this paper, we revise some of the most recent and promising modifications performed to chitosan with special focus on its employment in the pulmonary delivery of drugs.


## 2. Advantages of Chitosan for Pulmonary Delivery of Drugs

Chitosan possesses different beneficial properties that make it an attractive option for designing adequate dosage forms and advanced drug delivery systems to be administered to or through the lung. General advantages include the well-established biocompatibility and biodegradability of chitosan [4]. Moreover, antimicrobial [5] and antioxidant    [6, 7] activities have been reported for different types of chitosan and derivatives, which can also be regarded as potentially useful for the development of pulmonary drug delivery systems. The processability of chitosan and several derivatives allow obtaining different types of systems (powders or well-structured micro-  and nanocarriers) that can be optimized in order to present optimal aerodynamic particle diameters for lung deposition and retention    [8–11]. Also, the presence of reactive amine groups grants chitosan the chemical versatility for modification and functionalization (Figure 1) [12].Figure 1
Chemical structure of chitosan, comprisingN-acetyl-D-glucosamine (right) and D-glucosamine (left) units.Chitosan is a cationic mucoadhesive polymer. The ability to establish ionic, hydrogen, and hydrophobic bonding with negatively charged chains of mucin [13], the structural component of mucus fluids, evidences its potential for increasing lung retention of drug carriers comprising chitosan. The mucoadhesive properties of the native polymer can be further increased by chemical modification. In particular, thiolation (i.e., attachment of side chains containing thiol groups) has been proved an interesting strategy for this last purpose [14]. In one recent study, Makhlof et al.    [15] reported on the enhanced mucoadhesion of nanoparticles composed of thioglycolic acid-glycol chitosan, as compared to nanoparticles based on the nonthiolated polymer (i.e., glycol chitosan), after intratracheal administration to rats. More importantly, the increment in pulmonary mucoadhesion observed by these researchers was correlated with the greater bioavailability of calcitonin when this peptide was associated with nanoparticles. However, mucoadhesive properties of chitosan and derivatives may also be detrimental, since adhesion of delivery systems at the upper respiratory tract and airways can also occur, thus limiting the amount of carrier that effectively reaches the deep lung. Formulating scientists should keep this phenomenon in mind when developing chitosan-based systems.Chitosan is able to enhance absorption of drugs by the paracellular route, in particular macromolecules, due to the transiently disruption of tight junctions (Figure2) [16].Effect of chitosan on the absorption of drugs by the paracellular route. (a) Normal epithelium. (b) Transient disruption of tight junctions by chitosan with enhancement of drug absorption. 1: represents the drug, 2: represents the tight junction, and 3: represents chitosan.
(a)(b)In order to confirm this effect in pulmonary drug delivery, Yamamoto et al.    [17] performed in vivo experiments in guinea pigs by comparing the pulmonary absorption of different model drugs in solution (carboxyfluorescein and fluorescein isothiocyanate dextran with molecular weight (MW) varying from 4 to 70 kDa), either in the presence or absence of chitosan. Results showed significantly higher permeation for all the investigated model drugs in the presence of chitosan, as assessed by blood drug levels. Absorption enhancement was higher for higher MW isothiocyanate dextran, which is mainly absorbed by the paracellular route, providing evidence for the mechanism of intercellular tight junction disruption.Toxicological data on chitosan has been extensively reviewed and is regarded to be favorable when considering its use as a pharmaceutical excipient [4]. In the particular case of pulmonary drug delivery, in vitro cytotoxicity experiments were conducted for chitosan presenting different degrees of deacetylation and MW using human embryonic lung cells (L132 cells) [18]. Results revealed that chitosan presented 50% inhibitory concentration (IC50) values higher than 1 mg/mL. Studies have also been conducted for derivatives of chitosan or chitosan-based formulations intended for pulmonary administration. For instance, Grenha et al.    [19] showed that chitosan nanoparticles obtained by ionotropic gelation with tripolyphosphate and entrapped in mannitol microparticles presented reduced toxicity (concentrations of up to 1.3 mg/mL of nanoparticles were tested) to adenocarcinoma epithelial lung cell lines (Calu-3 and A549). In another study conducted in A549 cells, stearic acid-g-chitosan oligosaccharide micelles presented an IC50 value of approximately 369 μg/mL    [20]. However, chitosan and derivatives may also induce immune responses by lung cells/tissue. In an in vitrostudy, Calu-3 cells exposed to chitosan microparticles were able to elicit the release of inflammatory cytokines (IL-2 and IL-8) [21]. Florea et al.    [22] performed in vivostudies in rats by administering intratracheally chitosan and two brands of N-trimethylated chitosan (TMC), presenting different degrees of substitution (20% and 60%). Histopathological analysis of lung tissue showed that chitosan elicited neutrophil infiltration and structural damage in the lung parenchyma; however, this effect may be attributable not to the inherent toxicity of chitosan but most probably to the physical obstruction of the bronchioles due to higher viscosity of the chitosan formulation, thus causing local asphyxiation. In the case of TMC, these effects were milder, which could be associated with lower viscosity when compared to chitosan. Comparable inflammatory effects were also observed in rats after the intratracheal administration of chitosan microparticles [23]. Moreover, the production of several proinflammatory cytokines has been observed for hydrophobically modified glycol-chitosan nanoparticles in vivo after intratracheal instillation in mice [24]. Even if generally regarded as detrimental, immune stimulation may be a particularly interesting feature for vaccine development    [25].


## 3. Chitosan and Chitosan-Grafting Copolymers for Nanoparticle-Based Pulmonary Drug Delivery Systems

The first nanoparticles with drug delivery purposes began in the late 1960s [26]. Nanoparticles are solid colloidal particles made of macromolecular materials ranging in size from 1 to 1000 nm, although sometimes the term identifies particles in the 1 to 200 nm range, depending on the application field [27]. For pharmaceutical and medical applications, nanoparticles can be used therapeutically as carriers, either by dissolving, entrapping, or encapsulating the active substance (drug or biologically active material) or by adsorbing or attaching the active substance on the surface.Different nanoparticles have been developed for pulmonary administration of various drugs to treat diseases such as tuberculosis (TB)[28–30] and other pulmonary infections [31] and diseases [31, 32]. Since this natural polymer offers remarkable advantages over other natural and synthetic polymeric carriers, in this section, we will focus on chitosan-grafting-based polymeric nanoparticles as drug carriers.


### 3.1. Trimethyl Chitosan (TMC)

Trimethylated chitosan (TMC) is a partially quaternized chitosan derivative that is freely soluble in aqueous solutions over a wide range of pH as compared to other chitosan salt derivatives (Figure3). TMC is obtained by reductive methylation of chitosan using methyl iodide in the presence of a strong base (e.g., NaOH) at 60°C    [33, 34]. This soluble chitosan derivative has mucoadhesive properties and displays excellent absorption-enhancing properties, even at neutral pH    [35, 36]. This capability as enhancer is due to opening the tight junctions between adjacent epithelial cells through interactions between the protonated (positively charged) amino groups on the C-2 position and the negatively charged sites on the cell membrane and/or in the tight junctions    [37]; TMC has positive charges, independently of the pH, at all degrees of quaternization    [38]. An increase in the degree of quaternization leads to increase of the permeation-enhancing effect of TMC    [34].Figure 3
Chemical structure of trimethyl chitosan (showing the modification at the D-glucosamine unit).TMC-based nanoparticles have consistently shown their feasibility for mucosal immunization. Studies have addressed oral vaccination againstHelicobacter pylori using urease [39]. Intranasal administration of the influenza antigen elicited local and systemic immune responses in mice [40]. A similar approach was assessed with the tetanus toxoid by nasal instillation in mice [41]. TMC nanoparticles were loaded with FTIC ovalbumin, and their transport across the nasal epithelium of rat was studied [42]. A recent study has also shown that the delivery of the model antigen ovalbumin (OVA) to the cervical lymph nodes in a nanoconjugated form with TMC (~30 nm diameter) was twice more effective than the nasal administration of ovalbumin-containing TMC nanoparticles with size of one order of magnitude greater (~300 nm diameter) [43]. In turn, synergistic effects of the TLR9 ligand CpG in TMC particles have been reported after nasal vaccination [44].Florea et al. observed that 20% and 60% N-TMC (TMC20 and TMC60) enhanced the permeation of octreotidein vitro by 21-, 16-, and 30-fold. Also, the bioavailability was enhanced by 2.4-, 2.5-, and 3.9-fold, respectively. Cell viability and histology studies showed that the TMCs are safer than chitosan and that Calu-3 cell monolayers are a valuable model for predicting the paracellular transport kinetics in the airway epithelia. Taking into account these results, they sustain that cationic polysaccharides are promising enhancers for peptide drug absorption with prospect for sustained-release formulations [22]. Slutter et al. reported on the conjugation of an antigen, OVA, to TMC and the preparation of nanoparticles for subunit vaccination. The uptake of TMC-OVA conjugates by dendritic cells was similar to the uptake of TMC/OVA nanoparticles and over 5-fold greater when compared to a solution of OVA and TMC. Conjugation of the antigen to TMC and TMC/OVA is therefore a viable strategy to increase the immunogenicity of subunit vaccines [45].


### 3.2. Carboxymethyl Chitosan (CMC)

CMC is prepared by adding a carboxymethyl group in the structure of chitosan. This modification increases its solubility in neutral and basic solutions without affecting other important characteristics [46]. CMC is prepared by carboxymethylation of the hydroxyl and amine chitosan groups [47]. Different substitutions patterns can be obtained according to the reaction temperature used (Figure 4). At room temperature the O-substitution is favored, while at higher temperature the N-substitution is the preferred pathway. Taking into account reaction conditions and reagents, different derivatives can be produced, that is, N-, O-, N,O-, or N,N-dicarboxymethyl chitosan [48].Figure 4
Chemical structure of different types of carboxymethyl chitosan (CMC): (1)N-CMC, (2) N,N-CMC, (3) O-CMC, and (3) N,O-CMC (showing the modification at the D-glucosamine unit).CMC nanoparticles were prepared as carriers for some anticancer drugs. For example, Shi et al. used different kinds of CMC with various molecular weights and degrees of substitution to prepare nanoparticles through ionotropic gelification with calcium ions. These results showed the feasibility of CMC nanoparticles to entrap doxorubicin and the potential to deliver it following a controlled profile [49]. Anhitha et al. prepared curcumin-loaded O-CMC nanoparticles (curcumin-O-CMC Nps) as a novel carrier in cancer drug delivery applications. In L929, MCF-7, and PC-3 cell lines, the O-CMC NPs without drug showed no cytotoxicity, whereas curcumin and curcumin-O-CMC NPs resulted in considerable cell death. Cellular uptake was analyzed by fluorescence microscopy. Control cells without any exposure to NPs and cells incubated with O-CMC NPs showed no fluorescence. Conversely, cells incubated with curcumin-O-CMC NPs displayed green fluorescence, confirming the internalization of the particles [46]. In another work, tea polyphenols (TPs) were loaded into carboxymethyl chitosan and chitosan hydrochloride    [50]. In vitro studies showed that TPs were controlled released from nanoparticles in PBS at pH 7.4. In addition, the cell apoptosis rate was increased from 30% after 24 h to 62% at the end of 72 h, induced by loaded nanoparticles.CMC-based nanoparticles of varying average size (40–400 nm diameter) were developed for intranasal immunization [41]. An interesting finding of this study was that TMC-MCC composite nanoparticles obtained by electrostatic complexation of the two polymers with a positive surface charge exhibited higher immune responses when compared to chitosan, TMC, and MCC nanoparticles.Li et al. prepared nanoparticles with oleoyl-carboxymethyl chitosan encapsulating rifampicin as drug delivery systems [51]. These nanoparticles were not tested for any specific route of administration. But their pulmonary administration could be a possibility to treat tuberculosis. Therefore, it is necessary to carry out studies to test the feasibility of these NPs as inhaled drug delivery systems.


### 3.3. N-Succinyl-Chitosan (NSC)

NSC is a chitosan derivative obtained by the incorporation of succinyl moieties into the N-terminal group of the glucosamine units (Figure5) [52]. Like other derivatives, NSC displays good water solubility in a broad  pH range, and it is considered biocompatible both in vitro and in vivo. NSC was initially developed as a wound dressing material combined with collagen. It is also recognized as an excellent cosmetic ingredient (Moistfine liquid, INCI name Chitosan Succinamide).Figure 5
Chemical structure ofN-succinyl-chitosan (showing the modification at the D-glucosamine unit).Some authors have used this chitosan derivative to prepare anticancer-drug-loaded NPs. For example, Hou et al. synthesized a new NSC derivative by means of microwave irradiation. They entrapped successfully hydroxycamptothecin (HCPT) into the NSC nanoparticles and observed tumor targeting and significant suppression of tumor growth after s.c. injection (close to the tumor) in mice bearing S180 sarcoma tumor [53]. Yan et al. prepared similar nanoparticles loaded with 5-fluorouracil (5-FU) [54]. They evaluated biodistribution and tumor targeting after i.v. administration in Sarcoma 180-bearing mice. The 5-FU-loaded NPs were biodistributed mainly in the tumor and liver, being found small quantities in kidney and spleen [54]. Luo et al. evaluated antitumor effects of NSC nanoparticles (NSCNPs) without drugs in K562 cells    [55]. The results revealed that NSCNP could inhibit the proliferation of K562 with an IC50 of 37.78, 14.26, 10.93, and 9.78 υg/mL at 12, 24, 36, and 48 h, respectively. According to a cytomorphology study and the analysis of DNA fragments, the antitumor effect of NSCNP is achieved by necrosis and apoptosis induction in K562 cells.


### 3.4. PEGylated Chitosan

Grafting of hydrophilic polymers such as PEG onto chitosan is a well-known strategy to improve the solubility and biocompatibility of chitosan as well as to achieve lower recognition by the host immune system and increased blood circulation time (Figure6) [56]. These PEG chains create a barrier layer to prevent the adhesion of opsonins present in the blood, so that the particles can be “invisible” to phagocytic cells.Figure 6
PEG-g-chitosan as obtained by the two most commonly used synthetic routes: (1) reaction with an active ester derivative and (2) reductive amination. X: linker.Due to the advantages that PEG confers to chitosan, chitosan-g-PEG copolymer has been prepared and utilized to develop various types of nanocarriers for transmucosal drug delivery. Chitosan-g-PEG nanoparticles have been prepared by ionotropic gelation with TPP [57].  This system displayed a high association efficiency (>78.6%) leading to insulin loading values as high as 38.6%.  Results of in vivo studies after intranasal administration to healthy rabbits showed that the plasma glucose levels fell sharply and remained at a low concentration for, at most, 2-3 h and returned to baseline after 5 h. Other studies have shown that, apart from the reduction of the cytotoxicity, PEGylation of TMC led to improved colloidal stability of polyplexes and significantly increased cellular uptake compared to unmodified TMC [58]. These improvements resulted in a significant, up to 10-fold, increase of transfection efficiency in NIH/3T3, L929, and MeWo cells.Mao et al. prepared chitosan-DNA nanoparticles using a complex coacervation process [59]. They conjugated PEG on the nanoparticles and observed that the clearance of the PEGylated nanoparticles in male AKR/J mice (6–8 weeks) following intravenous administration was slower than that of unmodified nanoparticles at 15 min, with higher depositions in kidney and liver [59]. Other authors developed a functional nanoparticulate carrier for DNA transfection in asialoglycoprotein receptor overexpressed in HepG2 cells. For this, they grafted a methoxy PEG (MPEG) and a receptor ligand, lactobionic acid (LA) in chitosan [60]. They observed that the system with ligand chitosan-(O-MPEG)-(N-LA) showed better transfection efficiency (45.3%) than ligand-free chitosan-(O-MPEG) (19.8%).


### 3.5. Thiolated Chitosan

Thiol modifications to chitosan and nanoparticles derived from it have been aimed to localize a drug delivery system at a given target site (Figure7). Recently, it was documented that thiolated chitosan has strong mucoadhesive properties ascribed to the formation of disulfide bonds with cysteine-rich domains of mucus glycoproteins, leading to an improvement in mucoadhesion of up to 140-fold when compared to unmodified chitosan [61]. Insulin-loaded nanoparticles prepared with chitosan-N-acetyl-L-cysteine were found to improve the systemic absorption of insulin after nasal administration [62]. Although the exact mechanism for the enhanced effectiveness of this system remains unclear, it was speculated that the mucoadhesive and permeation-enhancing properties based on disulfide bond “anchorage” increase the accessibility of insulin molecules to the epithelial membrane and, hence, facilitates the absorption of the protein across the nasal epithelium.Figure 7
Thiolated chitosan: (1) general structure of thiolated chitosan as modified by an –SH group and (2) chitosan-N-acetyl-cysteine (showing the modification at the D-glucosamine unit). X: linker.


## 3.1. Trimethyl Chitosan (TMC)

Trimethylated chitosan (TMC) is a partially quaternized chitosan derivative that is freely soluble in aqueous solutions over a wide range of pH as compared to other chitosan salt derivatives (Figure3). TMC is obtained by reductive methylation of chitosan using methyl iodide in the presence of a strong base (e.g., NaOH) at 60°C    [33, 34]. This soluble chitosan derivative has mucoadhesive properties and displays excellent absorption-enhancing properties, even at neutral pH    [35, 36]. This capability as enhancer is due to opening the tight junctions between adjacent epithelial cells through interactions between the protonated (positively charged) amino groups on the C-2 position and the negatively charged sites on the cell membrane and/or in the tight junctions    [37]; TMC has positive charges, independently of the pH, at all degrees of quaternization    [38]. An increase in the degree of quaternization leads to increase of the permeation-enhancing effect of TMC    [34].Figure 3
Chemical structure of trimethyl chitosan (showing the modification at the D-glucosamine unit).TMC-based nanoparticles have consistently shown their feasibility for mucosal immunization. Studies have addressed oral vaccination againstHelicobacter pylori using urease [39]. Intranasal administration of the influenza antigen elicited local and systemic immune responses in mice [40]. A similar approach was assessed with the tetanus toxoid by nasal instillation in mice [41]. TMC nanoparticles were loaded with FTIC ovalbumin, and their transport across the nasal epithelium of rat was studied [42]. A recent study has also shown that the delivery of the model antigen ovalbumin (OVA) to the cervical lymph nodes in a nanoconjugated form with TMC (~30 nm diameter) was twice more effective than the nasal administration of ovalbumin-containing TMC nanoparticles with size of one order of magnitude greater (~300 nm diameter) [43]. In turn, synergistic effects of the TLR9 ligand CpG in TMC particles have been reported after nasal vaccination [44].Florea et al. observed that 20% and 60% N-TMC (TMC20 and TMC60) enhanced the permeation of octreotidein vitro by 21-, 16-, and 30-fold. Also, the bioavailability was enhanced by 2.4-, 2.5-, and 3.9-fold, respectively. Cell viability and histology studies showed that the TMCs are safer than chitosan and that Calu-3 cell monolayers are a valuable model for predicting the paracellular transport kinetics in the airway epithelia. Taking into account these results, they sustain that cationic polysaccharides are promising enhancers for peptide drug absorption with prospect for sustained-release formulations [22]. Slutter et al. reported on the conjugation of an antigen, OVA, to TMC and the preparation of nanoparticles for subunit vaccination. The uptake of TMC-OVA conjugates by dendritic cells was similar to the uptake of TMC/OVA nanoparticles and over 5-fold greater when compared to a solution of OVA and TMC. Conjugation of the antigen to TMC and TMC/OVA is therefore a viable strategy to increase the immunogenicity of subunit vaccines [45].


## 3.2. Carboxymethyl Chitosan (CMC)

CMC is prepared by adding a carboxymethyl group in the structure of chitosan. This modification increases its solubility in neutral and basic solutions without affecting other important characteristics [46]. CMC is prepared by carboxymethylation of the hydroxyl and amine chitosan groups [47]. Different substitutions patterns can be obtained according to the reaction temperature used (Figure 4). At room temperature the O-substitution is favored, while at higher temperature the N-substitution is the preferred pathway. Taking into account reaction conditions and reagents, different derivatives can be produced, that is, N-, O-, N,O-, or N,N-dicarboxymethyl chitosan [48].Figure 4
Chemical structure of different types of carboxymethyl chitosan (CMC): (1)N-CMC, (2) N,N-CMC, (3) O-CMC, and (3) N,O-CMC (showing the modification at the D-glucosamine unit).CMC nanoparticles were prepared as carriers for some anticancer drugs. For example, Shi et al. used different kinds of CMC with various molecular weights and degrees of substitution to prepare nanoparticles through ionotropic gelification with calcium ions. These results showed the feasibility of CMC nanoparticles to entrap doxorubicin and the potential to deliver it following a controlled profile [49]. Anhitha et al. prepared curcumin-loaded O-CMC nanoparticles (curcumin-O-CMC Nps) as a novel carrier in cancer drug delivery applications. In L929, MCF-7, and PC-3 cell lines, the O-CMC NPs without drug showed no cytotoxicity, whereas curcumin and curcumin-O-CMC NPs resulted in considerable cell death. Cellular uptake was analyzed by fluorescence microscopy. Control cells without any exposure to NPs and cells incubated with O-CMC NPs showed no fluorescence. Conversely, cells incubated with curcumin-O-CMC NPs displayed green fluorescence, confirming the internalization of the particles [46]. In another work, tea polyphenols (TPs) were loaded into carboxymethyl chitosan and chitosan hydrochloride    [50]. In vitro studies showed that TPs were controlled released from nanoparticles in PBS at pH 7.4. In addition, the cell apoptosis rate was increased from 30% after 24 h to 62% at the end of 72 h, induced by loaded nanoparticles.CMC-based nanoparticles of varying average size (40–400 nm diameter) were developed for intranasal immunization [41]. An interesting finding of this study was that TMC-MCC composite nanoparticles obtained by electrostatic complexation of the two polymers with a positive surface charge exhibited higher immune responses when compared to chitosan, TMC, and MCC nanoparticles.Li et al. prepared nanoparticles with oleoyl-carboxymethyl chitosan encapsulating rifampicin as drug delivery systems [51]. These nanoparticles were not tested for any specific route of administration. But their pulmonary administration could be a possibility to treat tuberculosis. Therefore, it is necessary to carry out studies to test the feasibility of these NPs as inhaled drug delivery systems.


## 3.3. N-Succinyl-Chitosan (NSC)

NSC is a chitosan derivative obtained by the incorporation of succinyl moieties into the N-terminal group of the glucosamine units (Figure5) [52]. Like other derivatives, NSC displays good water solubility in a broad  pH range, and it is considered biocompatible both in vitro and in vivo. NSC was initially developed as a wound dressing material combined with collagen. It is also recognized as an excellent cosmetic ingredient (Moistfine liquid, INCI name Chitosan Succinamide).Figure 5
Chemical structure ofN-succinyl-chitosan (showing the modification at the D-glucosamine unit).Some authors have used this chitosan derivative to prepare anticancer-drug-loaded NPs. For example, Hou et al. synthesized a new NSC derivative by means of microwave irradiation. They entrapped successfully hydroxycamptothecin (HCPT) into the NSC nanoparticles and observed tumor targeting and significant suppression of tumor growth after s.c. injection (close to the tumor) in mice bearing S180 sarcoma tumor [53]. Yan et al. prepared similar nanoparticles loaded with 5-fluorouracil (5-FU) [54]. They evaluated biodistribution and tumor targeting after i.v. administration in Sarcoma 180-bearing mice. The 5-FU-loaded NPs were biodistributed mainly in the tumor and liver, being found small quantities in kidney and spleen [54]. Luo et al. evaluated antitumor effects of NSC nanoparticles (NSCNPs) without drugs in K562 cells    [55]. The results revealed that NSCNP could inhibit the proliferation of K562 with an IC50 of 37.78, 14.26, 10.93, and 9.78 υg/mL at 12, 24, 36, and 48 h, respectively. According to a cytomorphology study and the analysis of DNA fragments, the antitumor effect of NSCNP is achieved by necrosis and apoptosis induction in K562 cells.


## 3.4. PEGylated Chitosan

Grafting of hydrophilic polymers such as PEG onto chitosan is a well-known strategy to improve the solubility and biocompatibility of chitosan as well as to achieve lower recognition by the host immune system and increased blood circulation time (Figure6) [56]. These PEG chains create a barrier layer to prevent the adhesion of opsonins present in the blood, so that the particles can be “invisible” to phagocytic cells.Figure 6
PEG-g-chitosan as obtained by the two most commonly used synthetic routes: (1) reaction with an active ester derivative and (2) reductive amination. X: linker.Due to the advantages that PEG confers to chitosan, chitosan-g-PEG copolymer has been prepared and utilized to develop various types of nanocarriers for transmucosal drug delivery. Chitosan-g-PEG nanoparticles have been prepared by ionotropic gelation with TPP [57].  This system displayed a high association efficiency (>78.6%) leading to insulin loading values as high as 38.6%.  Results of in vivo studies after intranasal administration to healthy rabbits showed that the plasma glucose levels fell sharply and remained at a low concentration for, at most, 2-3 h and returned to baseline after 5 h. Other studies have shown that, apart from the reduction of the cytotoxicity, PEGylation of TMC led to improved colloidal stability of polyplexes and significantly increased cellular uptake compared to unmodified TMC [58]. These improvements resulted in a significant, up to 10-fold, increase of transfection efficiency in NIH/3T3, L929, and MeWo cells.Mao et al. prepared chitosan-DNA nanoparticles using a complex coacervation process [59]. They conjugated PEG on the nanoparticles and observed that the clearance of the PEGylated nanoparticles in male AKR/J mice (6–8 weeks) following intravenous administration was slower than that of unmodified nanoparticles at 15 min, with higher depositions in kidney and liver [59]. Other authors developed a functional nanoparticulate carrier for DNA transfection in asialoglycoprotein receptor overexpressed in HepG2 cells. For this, they grafted a methoxy PEG (MPEG) and a receptor ligand, lactobionic acid (LA) in chitosan [60]. They observed that the system with ligand chitosan-(O-MPEG)-(N-LA) showed better transfection efficiency (45.3%) than ligand-free chitosan-(O-MPEG) (19.8%).


## 3.5. Thiolated Chitosan

Thiol modifications to chitosan and nanoparticles derived from it have been aimed to localize a drug delivery system at a given target site (Figure7). Recently, it was documented that thiolated chitosan has strong mucoadhesive properties ascribed to the formation of disulfide bonds with cysteine-rich domains of mucus glycoproteins, leading to an improvement in mucoadhesion of up to 140-fold when compared to unmodified chitosan [61]. Insulin-loaded nanoparticles prepared with chitosan-N-acetyl-L-cysteine were found to improve the systemic absorption of insulin after nasal administration [62]. Although the exact mechanism for the enhanced effectiveness of this system remains unclear, it was speculated that the mucoadhesive and permeation-enhancing properties based on disulfide bond “anchorage” increase the accessibility of insulin molecules to the epithelial membrane and, hence, facilitates the absorption of the protein across the nasal epithelium.Figure 7
Thiolated chitosan: (1) general structure of thiolated chitosan as modified by an –SH group and (2) chitosan-N-acetyl-cysteine (showing the modification at the D-glucosamine unit). X: linker.


## 4. Chitosan-Based Polymeric Micelles for Pulmonary Drug Delivery

Micelles are spherical nanosized colloidal dispersions with a hydrophobic core and hydrophilic shell, composed by amphiphilic molecules which self-assembly under certain concentration and temperature conditions (Figure8). The concentration and the temperature above which the monomers turn in micelles are called critical micelle concentration (CMC) and critical micellization temperature (CMT), respectively [63]. The self-assembly of amphiphilic molecules in water is driven by a gain in entropy of the solvent molecules and a decrease of free energy in the system as the hydrophobic components withdraw from the aqueous media [64]. Two forces are involved in the micelle formation, an attractive force that leads to the association of molecules and a repulsive force that prevents unlimited growth of the micelles [65]. Micelles can be used as drug delivery systems, especially for poorly water-soluble drugs that are incorporated into the micelle core. Like liposomes, polymeric micelles allow the encapsulation of drugs with different polarities. Water-soluble drugs are adsorbed on the micelle surface, and drugs with intermediate polarity are distributed along the amphiphilic molecules [66]. However, they are more stable than liposomes. Furthermore, due to its general small size, micelles have the ability to penetrate tissues, showing also a high encapsulation efficiency and possibility of sterilization by filtration    [63, 66, 67].Figure 8
Formation of micelles by self-assembly of polymer monomers above critical micelle concentration (CMC) and critical micellization temperature (CMT).Recently, much attention has been given to polymeric micelles formed by lipid polymer or block copolymers, generally di- and triblock copolymers, as drug delivery systems. Compared to micelles prepared from conventional detergents, polymeric micelles present a lower CMC value, making them more physically stable, bothin vitro and in vivo, even under dilutions to final concentration below the CMC [68]. Many studies have been published in the last years regarding the use of polymeric micelles as solubilization agents and bioavailability enhancers of different drugs, with emphasis on poorly soluble anticancer drugs    [69–73]. They have also been studied as carriers of genetic material [74] and diagnostic agents [75]. Due to its size, generally lower than 200 nm, and hydrophilic surface, micelles are poorly recognized by the reticuloendothelial system (RES) and present long circulation times in bloodstream with enhanced permeability and retention effects (EPR effect) at solid tumor sites or another areas with leaky vasculature such infarcts, infections, and inflammations, for passive targeting    [76, 77]. It is also possible to promote the targeting of drugs to specific organs and tissues using stimuli-responsive micelles    [77–79] or modulating the micellar surface with active-targeting ligands    [77, 80]. By sharing some structural and functional features with natural transport systems, for example, virus and lipoproteins, polymeric micelles can be a useful strategy to solve the problem of drug resistance [81].Different polymeric micelles have been developed, in the last years, for pulmonary administration of various drugs to treat different diseases such as cancer [82], inflammation conditions [83], and pulmonary infections    [84–86]. They have been also studied as carriers of genetic material to treat cystic fibrosis [87]. In this section, we will focus on chitosan-based polymeric micelles as drug carriers.


### 4.1. Chitosan-Based Micelles

Due to its cationic nature, chitosan can be complexed with negatively charged DNA and be used as nonviral vector for gene therapy. Hu and coworkers synthesized stearic acid- (SA-) grafted chitosan oligosaccharide (CSO) in order to produce polymeric micelles to delivery pDNA (pEGFP-C1) [88]. The SA-CSO/DNA micelles efficiently protected the condensed DNA from enzymatic degradation by DNAse I and  presented lower cytotoxicity and comparable transfection efficiency in A549 cells compared to Lipofectamine 2000 [88], making these micelles a promising gene delivery system in the treatment of pulmonary diseases. The same research group developed SA-CSO micelles for the delivery of drugs like paclitaxel [89], doxorubicin    [90, 91], and proteins [92]. The encapsulation of doxorubicin in SA-CSO micelles resulted in higher uptake and accumulation by A549 cells and a decrease in the IC50 [20]. Recently, SA-CSO micelles were developed by another research group, for the pulmonary administration of amphotericin B (AmB) [93]. The local administration of AmB to treat invasive pulmonary fungal infections, present in some patients receiving immune suppressive treatments, avoids the systemic side effects and improves the bioavailability of the drug [94]. After encapsulation into micelles, AmB presents the same antifungal activity of Fungizone but lower toxicity [93], a phenomenon intimately related to its aggregation state [95]. The micelles possessed positive charges with mean diameters between 100 and 250 nm and were efficiently nebulized using an Air-jet nebulizer presenting up to 52% of fine particle fraction, making them a suitable choice for pulmonary delivery of AmB [93].In the field of block copolymers, Liu and coworkers developed an triblock copolymer consisting of poly(ε-caprolactone)-b-chitooligosaccharide-b-poly(ethylene glycol) (PCL-b-COS-b-PEG) for delivery of drugs, using doxorubicin as model drug [96]. The obtained polymer presents the capacity to form micelles with encapsulation efficiency of doxorubicin close to 50%. Genipin after crosslinking did not affect the macroscopic characteristics of the micelles but delayed the in vitro release of doxorubicin from the micellar reservoir [96]. Similar results were obtained by Chen et al. using chitosan-poly(ε-caprolactone)-poly(ethylene glycol) to encapsulate paclitaxel and rutin with glutaraldehyde after crosslinking [97]. Wu and co-workers also synthesized chitosan-based copolymers for drug delivery [98]. Polylactide- (PLA-) chitosan copolymers with different molar ratios were developed and characterized. Rifampicin was used as lipophilic drug model to be encapsulated. As PLA molar ratio increased, the micelle size and drug-loading content increased, and the rifampicin release rate decreased [98]. The present systems were not tested for any specific route of administration.Besides the creation of lipid-chitosan and polymer-chitosan conjugates, it is also possible to produce chitosan derivatives with amphiphilic characteristics that may self-assemble in aqueous environment and form polymeric micelles    [99–101]. Zhang and co-workers synthesized different chitosan derivatives composed by long-chain alkyl groups as hydrophobic moieties and  sulfated groups as hydrophilic moieties to delivery of paclitaxel [102]. Between the different derivatives, N-octyl-O-sulfate chitosan presented the best results in terms of solubilization of paclitaxel [102]. Paclitaxel-loaded micelles prepared with N-octyl-O-sulfate chitosan had high drug-loading capacity and encapsulation efficiency, were shown to be safe for intravenous injection, and presented similar antitumor efficacy as Taxol, but significantly reduced toxicity and improved bioavailability    [103]. They also synthesized N-alkyl-N-trimethyl chitosan derivatives to deliver 10-hydroxycamptothecin. The best results in terms of encapsulation efficiency, stability, release behavior, and pharmacokinetic properties were obtained with N-octyl-N-trimethyl chitosan (degree of octyl and trimethyl substitution is 8% and 54%, resp.) [104]. Another research group studied the feasibility of N-succinyl-N-octyl chitosan as delivery system of doxorubicin [105]. Although these systems have been tested for intravenous administration, they present antitumor efficacy against lung cancer cell lines such as Lewis lung cancer cells    [103] and A549 cells    [103, 105]. Their pulmonary administration could be a possibility to treat locally lung cancer; however, studies are required to access its feasibility as inhaled drug delivery systems.Chitosan-based polymeric micelles have also been developed as drug delivery systems for other routes of administration than pulmonary, with emphasis on intravenous administration of paclitaxel. Examples are the N-mPEG-N-octyl-O-sulfate chitosan micelles produced by Qu and co-workers [106], the N-lauryl-carboxymethyl-chitosan micelles developed by Miwa et al. [107], and the N-octyl-N-(2-carboxyl-cyclohexamethenyl) chitosan micelles produced by Liu and co-workers [108]. Other chitosan-based micelles have been developed such as the doxorubicin-loaded linoleic acid-grafted chitosan oligosaccharide micelles produced by Du et al. for intercellular antitumor drug delivery to drug resistance tumor cells    [109, 110] or the methoxy poly(ethylene glycol)-grafted chitosan micelles for delivery of methotrexate to treat colon carcinoma    [111, 112].


## 4.1. Chitosan-Based Micelles

Due to its cationic nature, chitosan can be complexed with negatively charged DNA and be used as nonviral vector for gene therapy. Hu and coworkers synthesized stearic acid- (SA-) grafted chitosan oligosaccharide (CSO) in order to produce polymeric micelles to delivery pDNA (pEGFP-C1) [88]. The SA-CSO/DNA micelles efficiently protected the condensed DNA from enzymatic degradation by DNAse I and  presented lower cytotoxicity and comparable transfection efficiency in A549 cells compared to Lipofectamine 2000 [88], making these micelles a promising gene delivery system in the treatment of pulmonary diseases. The same research group developed SA-CSO micelles for the delivery of drugs like paclitaxel [89], doxorubicin    [90, 91], and proteins [92]. The encapsulation of doxorubicin in SA-CSO micelles resulted in higher uptake and accumulation by A549 cells and a decrease in the IC50 [20]. Recently, SA-CSO micelles were developed by another research group, for the pulmonary administration of amphotericin B (AmB) [93]. The local administration of AmB to treat invasive pulmonary fungal infections, present in some patients receiving immune suppressive treatments, avoids the systemic side effects and improves the bioavailability of the drug [94]. After encapsulation into micelles, AmB presents the same antifungal activity of Fungizone but lower toxicity [93], a phenomenon intimately related to its aggregation state [95]. The micelles possessed positive charges with mean diameters between 100 and 250 nm and were efficiently nebulized using an Air-jet nebulizer presenting up to 52% of fine particle fraction, making them a suitable choice for pulmonary delivery of AmB [93].In the field of block copolymers, Liu and coworkers developed an triblock copolymer consisting of poly(ε-caprolactone)-b-chitooligosaccharide-b-poly(ethylene glycol) (PCL-b-COS-b-PEG) for delivery of drugs, using doxorubicin as model drug [96]. The obtained polymer presents the capacity to form micelles with encapsulation efficiency of doxorubicin close to 50%. Genipin after crosslinking did not affect the macroscopic characteristics of the micelles but delayed the in vitro release of doxorubicin from the micellar reservoir [96]. Similar results were obtained by Chen et al. using chitosan-poly(ε-caprolactone)-poly(ethylene glycol) to encapsulate paclitaxel and rutin with glutaraldehyde after crosslinking [97]. Wu and co-workers also synthesized chitosan-based copolymers for drug delivery [98]. Polylactide- (PLA-) chitosan copolymers with different molar ratios were developed and characterized. Rifampicin was used as lipophilic drug model to be encapsulated. As PLA molar ratio increased, the micelle size and drug-loading content increased, and the rifampicin release rate decreased [98]. The present systems were not tested for any specific route of administration.Besides the creation of lipid-chitosan and polymer-chitosan conjugates, it is also possible to produce chitosan derivatives with amphiphilic characteristics that may self-assemble in aqueous environment and form polymeric micelles    [99–101]. Zhang and co-workers synthesized different chitosan derivatives composed by long-chain alkyl groups as hydrophobic moieties and  sulfated groups as hydrophilic moieties to delivery of paclitaxel [102]. Between the different derivatives, N-octyl-O-sulfate chitosan presented the best results in terms of solubilization of paclitaxel [102]. Paclitaxel-loaded micelles prepared with N-octyl-O-sulfate chitosan had high drug-loading capacity and encapsulation efficiency, were shown to be safe for intravenous injection, and presented similar antitumor efficacy as Taxol, but significantly reduced toxicity and improved bioavailability    [103]. They also synthesized N-alkyl-N-trimethyl chitosan derivatives to deliver 10-hydroxycamptothecin. The best results in terms of encapsulation efficiency, stability, release behavior, and pharmacokinetic properties were obtained with N-octyl-N-trimethyl chitosan (degree of octyl and trimethyl substitution is 8% and 54%, resp.) [104]. Another research group studied the feasibility of N-succinyl-N-octyl chitosan as delivery system of doxorubicin [105]. Although these systems have been tested for intravenous administration, they present antitumor efficacy against lung cancer cell lines such as Lewis lung cancer cells    [103] and A549 cells    [103, 105]. Their pulmonary administration could be a possibility to treat locally lung cancer; however, studies are required to access its feasibility as inhaled drug delivery systems.Chitosan-based polymeric micelles have also been developed as drug delivery systems for other routes of administration than pulmonary, with emphasis on intravenous administration of paclitaxel. Examples are the N-mPEG-N-octyl-O-sulfate chitosan micelles produced by Qu and co-workers [106], the N-lauryl-carboxymethyl-chitosan micelles developed by Miwa et al. [107], and the N-octyl-N-(2-carboxyl-cyclohexamethenyl) chitosan micelles produced by Liu and co-workers [108]. Other chitosan-based micelles have been developed such as the doxorubicin-loaded linoleic acid-grafted chitosan oligosaccharide micelles produced by Du et al. for intercellular antitumor drug delivery to drug resistance tumor cells    [109, 110] or the methoxy poly(ethylene glycol)-grafted chitosan micelles for delivery of methotrexate to treat colon carcinoma    [111, 112].


## 5. Chitosan-Ligand Conjugates for Nanoparticle-Based Active Target Drug Delivery Systems

As discussed in previous sections, chitosan is an ideal natural polymer for the design and development of drug delivery systems structured at micro- and nanoscopic scales. Particularly, its mucoadhesiveness [113, 114], biocompatibility [115, 116], and capacity to promote the absorption of poorly absorbable macromolecules across epithelial barriers by transient widening of cell tight junctions thus modifying the parallel transport    [117–120] have been exploited in the development of nanocarrier systems for transmucosal delivery. A recognized feature of chitosan-based nanostructured systems is their capacity to protect sensitive therapeutic macromolecules against degradation and their ability to overcome mucosal barriers. As a consequence, their application has been centered particularly in noninvasive routes of administration including transmucosal administration of proteins    [121–125] and genetic material    [126–130].The capacity of chitosan to undergo multiple chemical modifications has been exploited to increase the active targeting of chitosan-based nanocarriers particularly for protein and genetic material delivery towards specific cells    [126, 131]. The modifications have comprised from derivatization with small functional groups or substructures (e.g., thiolated derivatives, grafted PEG) to conjugation with biologically active ligands such as carbohydrates (e.g., galactose, mannose) or specific ligands such as folate, transferrin, or KNOB viral protein. Nanocarrier systems of this kind with improved biological and biopharmaceutical performance have been the subject of active research in the past decade or so, as discussed, with reference to specific systems, in the following sections.


### 5.1. Conjugation of Sugar Ligands

Strategies to improve the targeting potential of chitosan have addressed its functionalization with sugar moieties, mostly with D-galactose and D-mannose. Galactose groups were chemically bound to chitosan aiming to achieve liver target delivery, while dextran was grafted to enhance the stability of the complex in aqueous media. The system was found efficient to transfect liver cells expressing asialoglycoprotein receptor (ASGRr), which specifically recognizes the galactose ligands on modified chitosan [132]. Galactosylated chitosan-graft-PEG (GCP) was developed for the same purpose. GCP-DNA complexes were found to be stable due to hydrophobic shielding by PEG and increased the stability against DNAse degradation. This system was found to enhance the transfection of HepG2 cells having ASGRr, thus indicating that galactosylated chitosan is an effective hepatocyte-targeted gene carrier [132]. This same system was tested in vivo by a different group. Glycoconjugated chitosan was designed for ASGRr-directed delivery to liver parenchymal cells. It was found that this system has the potential to be a vector for targeting to Kupffer cells in vivo.  Other chitosan glycoconjugates have incorporated lactose to develop gene nanocarriers. HeLa cells were effectively transfected by this nanocarrier system, but neither HepG2 nor BNL CL2 cells. TEM evidence was consistent with the proposal that the nanocomplexes were internalized by HeLa cells and located inside endocytotic vesicles and endosome-like compartments [133]. In turn, efforts have been made to target mannose receptors on dendritic cells residing in tumors; hence, chitosan has been functionalized with mannose. Using these nanoparticles in the delivery of a plasmid encoding IL-12 resulted in enhanced IL-12 gene transfer efficiency, suppressed tumour growth and angiogenesis in the carcinoma BALB/c mouse model [134]. A trisaccharide branch was attached onto chitosan chain in order to target lectins on the cell surface in lung tissues [135]. This modification increased the carrier uptake and transfection efficiencies in various in vitro assays as well as in mouse lung tissue as recently reviewed elsewhere [136].


### 5.2. Conjugation of Folate

Folic acid (FA) is appealing as a ligand for targeting cell membrane and allowing nanoparticle endocytosis via the folate receptor (FR) for higher transfection yields. Importantly, the high affinity of folate to bind its receptor (1 nM) [137] and folate small size allows its use for specific cell targeting. Moreover, the ability of FA to bind its receptor to allow endocytosis is not altered by covalent conjugation of small molecules [138]. Folate receptor (FR) is over-expressed on many human cancer cell surfaces,  and the nonepithelial isoform of FR (FRb) is expressed on activated synovial macrophages present in large numbers in arthritic joints [139]. Hence, folate conjugation to the surface of chitosan and chitosan-derivatives-based nanoparticles has been one of the actively studied strategies to vectorize drugs over the past few years    [140–149]. These systems have been developed with a view to achieve targeting effect in the delivery of cytostatic drugs to tumor cells, genetic material, or antiarthritis therapies and also for diagnostic and imaging purposes. To this end, the majority of in vitro studies have been conducted in various types of cell lines well known to overexpress the human folate receptors (FRa and FLRb), such as HeLa [145], HT29 [143], Caco-2 [143], B16F1 [150], KB    [151, 152], HepG-2     [153], and SKOV3    [141, 154] cells.The evidence from most of these studies is consistent to indicate that the folic acid modification promotes the uptake of nanoparticles by FR-positive tumor cell lines most likely via receptor-mediated endocytosis but has little impact on other cells without FR    [155]. Results of transfection studies showed that folate-chitosan-based nanoparticles enhanced the reporter gene expression against a cell line overexpressing FR (SKOV3 cells) compared to an FR-deficient cell line (A549 cells) and did not induce obvious cytotoxicity against HEK 293 cells [154]. In turn, NPs made out of folate-grafted chitosan were produced to transfect interleukin-1 receptor antagonist (IL-1Ra) in synovial mononuclear cells and CD14+ cells via the targeting of the folate receptor-b    [156]. Compared to unmodified chitosan or naked DNA, this system allowed for an increase in IL-1Ra expression combined with a diminution of cytotoxicity in vitro and reinforced protection against inflammation and abnormal bone metabolismin vivo.


### 5.3. Conjugation of Protein Ligands

Proteins, such as transferrin and viral KNOB, have been conjugated at the surface of chitosan-based nanoparticles intended for DNA delivery, as a strategy to achieve active targeting and thus high transfection efficiency    [59, 157]. The transferrin receptor (TfR) is found in many mammalian cells, responsible for iron import to cells, and it is known to enhance the transcytosis of viral vector [158, 159]. Transferrin (Tf) or antibodies against TfR were conjugated to oligonucleotides or polycations, which then complexed with pcDNA. Two different synthetic approaches were tested to couple Tf to the surface of chitosan nanoparticles, achieving a conjugation in both cases about 33–43% (transferrin to chitosan moL%) [59]. In this study HEK293 cells were transfected using luciferase reporter gene. Tf-conjugated nanoparticles invariably showed threefold greater transfection efficiency than unmodified nanoparticles. However, the Tf-decorated nanoparticles did not show significant enhancement of the transfection of HeLa cells [160].Tf decoration of chitosan-based DNA-loaded nanoparticles has been found to enhance the transport across polarized monolayer Caco-2 cells known to have abundant Tf receptors on their surface [159] and  extensively used as a model of normal intestinal epithelium transport [161]. It was demonstrated that Tf conjugation could enhance the transport of nanoparticles through Caco-2 alone and Caco-2-PPL cocultures by 3- to 5-fold. One drawback of these systems is that they only proved to be ineffective in the presence of added serum medium. An interesting contribution of this study was to address the behavior of a coculture of polarized Caco-2 cells infiltrated with lymphocytes that induces the differentiation of M-type phenotype, a model much closer to the real intestinal epithelium. The results led to suggest that uptake in the lymphoid follicles of the duodenum could play a more significant role compared to Peyer’s patches [157].A second related strategy has been to decorate the surface of DNA-loaded chitosan-based nanoparticles with KNOB protein so as to enhance the uptake and overcome one of the major rate-limiting steps for transfection mediated by chitosan nanoparticles via a specific receptor-mediated endocytosis mechanism [59]. KNOB conjugation to the nanoparticles significantly improved the transfection efficiency (increased 130 times) when transfecting HeLa cells but still not to a level that could rival Lipofectamine transfection.


### 5.4. Hybrid Nanoparticles Comprising Other Polysaccharides

Yet another approach to enhance the targeting capacity of chitosan-based nanocapsules has been to develop hybrid co-gelled systems including other polysaccharides with known affinity toward specific receptors. Among these, nanoparticles comprising hyaluronan, alginate, and glucomannan have deserved attention, particularly for the delivery of insulin, vaccines, and DNA via transmucosa. The reader is referred to recent comprehensive reviews on the subject    [125, 127, 162].


## 5.1. Conjugation of Sugar Ligands

Strategies to improve the targeting potential of chitosan have addressed its functionalization with sugar moieties, mostly with D-galactose and D-mannose. Galactose groups were chemically bound to chitosan aiming to achieve liver target delivery, while dextran was grafted to enhance the stability of the complex in aqueous media. The system was found efficient to transfect liver cells expressing asialoglycoprotein receptor (ASGRr), which specifically recognizes the galactose ligands on modified chitosan [132]. Galactosylated chitosan-graft-PEG (GCP) was developed for the same purpose. GCP-DNA complexes were found to be stable due to hydrophobic shielding by PEG and increased the stability against DNAse degradation. This system was found to enhance the transfection of HepG2 cells having ASGRr, thus indicating that galactosylated chitosan is an effective hepatocyte-targeted gene carrier [132]. This same system was tested in vivo by a different group. Glycoconjugated chitosan was designed for ASGRr-directed delivery to liver parenchymal cells. It was found that this system has the potential to be a vector for targeting to Kupffer cells in vivo.  Other chitosan glycoconjugates have incorporated lactose to develop gene nanocarriers. HeLa cells were effectively transfected by this nanocarrier system, but neither HepG2 nor BNL CL2 cells. TEM evidence was consistent with the proposal that the nanocomplexes were internalized by HeLa cells and located inside endocytotic vesicles and endosome-like compartments [133]. In turn, efforts have been made to target mannose receptors on dendritic cells residing in tumors; hence, chitosan has been functionalized with mannose. Using these nanoparticles in the delivery of a plasmid encoding IL-12 resulted in enhanced IL-12 gene transfer efficiency, suppressed tumour growth and angiogenesis in the carcinoma BALB/c mouse model [134]. A trisaccharide branch was attached onto chitosan chain in order to target lectins on the cell surface in lung tissues [135]. This modification increased the carrier uptake and transfection efficiencies in various in vitro assays as well as in mouse lung tissue as recently reviewed elsewhere [136].


## 5.2. Conjugation of Folate

Folic acid (FA) is appealing as a ligand for targeting cell membrane and allowing nanoparticle endocytosis via the folate receptor (FR) for higher transfection yields. Importantly, the high affinity of folate to bind its receptor (1 nM) [137] and folate small size allows its use for specific cell targeting. Moreover, the ability of FA to bind its receptor to allow endocytosis is not altered by covalent conjugation of small molecules [138]. Folate receptor (FR) is over-expressed on many human cancer cell surfaces,  and the nonepithelial isoform of FR (FRb) is expressed on activated synovial macrophages present in large numbers in arthritic joints [139]. Hence, folate conjugation to the surface of chitosan and chitosan-derivatives-based nanoparticles has been one of the actively studied strategies to vectorize drugs over the past few years    [140–149]. These systems have been developed with a view to achieve targeting effect in the delivery of cytostatic drugs to tumor cells, genetic material, or antiarthritis therapies and also for diagnostic and imaging purposes. To this end, the majority of in vitro studies have been conducted in various types of cell lines well known to overexpress the human folate receptors (FRa and FLRb), such as HeLa [145], HT29 [143], Caco-2 [143], B16F1 [150], KB    [151, 152], HepG-2     [153], and SKOV3    [141, 154] cells.The evidence from most of these studies is consistent to indicate that the folic acid modification promotes the uptake of nanoparticles by FR-positive tumor cell lines most likely via receptor-mediated endocytosis but has little impact on other cells without FR    [155]. Results of transfection studies showed that folate-chitosan-based nanoparticles enhanced the reporter gene expression against a cell line overexpressing FR (SKOV3 cells) compared to an FR-deficient cell line (A549 cells) and did not induce obvious cytotoxicity against HEK 293 cells [154]. In turn, NPs made out of folate-grafted chitosan were produced to transfect interleukin-1 receptor antagonist (IL-1Ra) in synovial mononuclear cells and CD14+ cells via the targeting of the folate receptor-b    [156]. Compared to unmodified chitosan or naked DNA, this system allowed for an increase in IL-1Ra expression combined with a diminution of cytotoxicity in vitro and reinforced protection against inflammation and abnormal bone metabolismin vivo.


## 5.3. Conjugation of Protein Ligands

Proteins, such as transferrin and viral KNOB, have been conjugated at the surface of chitosan-based nanoparticles intended for DNA delivery, as a strategy to achieve active targeting and thus high transfection efficiency    [59, 157]. The transferrin receptor (TfR) is found in many mammalian cells, responsible for iron import to cells, and it is known to enhance the transcytosis of viral vector [158, 159]. Transferrin (Tf) or antibodies against TfR were conjugated to oligonucleotides or polycations, which then complexed with pcDNA. Two different synthetic approaches were tested to couple Tf to the surface of chitosan nanoparticles, achieving a conjugation in both cases about 33–43% (transferrin to chitosan moL%) [59]. In this study HEK293 cells were transfected using luciferase reporter gene. Tf-conjugated nanoparticles invariably showed threefold greater transfection efficiency than unmodified nanoparticles. However, the Tf-decorated nanoparticles did not show significant enhancement of the transfection of HeLa cells [160].Tf decoration of chitosan-based DNA-loaded nanoparticles has been found to enhance the transport across polarized monolayer Caco-2 cells known to have abundant Tf receptors on their surface [159] and  extensively used as a model of normal intestinal epithelium transport [161]. It was demonstrated that Tf conjugation could enhance the transport of nanoparticles through Caco-2 alone and Caco-2-PPL cocultures by 3- to 5-fold. One drawback of these systems is that they only proved to be ineffective in the presence of added serum medium. An interesting contribution of this study was to address the behavior of a coculture of polarized Caco-2 cells infiltrated with lymphocytes that induces the differentiation of M-type phenotype, a model much closer to the real intestinal epithelium. The results led to suggest that uptake in the lymphoid follicles of the duodenum could play a more significant role compared to Peyer’s patches [157].A second related strategy has been to decorate the surface of DNA-loaded chitosan-based nanoparticles with KNOB protein so as to enhance the uptake and overcome one of the major rate-limiting steps for transfection mediated by chitosan nanoparticles via a specific receptor-mediated endocytosis mechanism [59]. KNOB conjugation to the nanoparticles significantly improved the transfection efficiency (increased 130 times) when transfecting HeLa cells but still not to a level that could rival Lipofectamine transfection.


## 5.4. Hybrid Nanoparticles Comprising Other Polysaccharides

Yet another approach to enhance the targeting capacity of chitosan-based nanocapsules has been to develop hybrid co-gelled systems including other polysaccharides with known affinity toward specific receptors. Among these, nanoparticles comprising hyaluronan, alginate, and glucomannan have deserved attention, particularly for the delivery of insulin, vaccines, and DNA via transmucosa. The reader is referred to recent comprehensive reviews on the subject    [125, 127, 162].


## 6. Conclusion

Pondering advantages and drawbacks, chitosan-based materials represent a very versatile and appealing technological platform to address the design of drug delivery systems that combine unique features such as mucoadhesiveness and enhanced drug absorption for the localized and systemic delivery of drugs through the respiratory system. Furthermore, in a more global perspective that values the potential of the bench-to-bedside translation, relatively low price, commercial availability in a broad spectrum of molecular weights and degrees of deacetylation and chemical versatility are outstanding properties that make of chitosan an excellent candidate in an industrial setting. On the other hand, the lack of reproducibility between batches usually displayed by natural polymers might lead to a less robust production process and remains a challenge to be addressed and overcome. Finally, to date chitosan is approved only as food supplement classified as a “generally recognized as safe” (GRAS) material [4]. In the context of pharmaceutical and biomedical products, a diversity of systems (e.g., micro- and nanoparticles, films, scaffolds, etc.) is under investigation. Remarkably, none of them has been approved by regulatory agencies yet. The diversity of molecular aspects that might affect the biodegradability, biodistribution, and biocompatibility needs to be carefully investigated as straight extrapolations among derivatives seem very complex, if not impossible. Hence, the regulatory phase will also need to be comprehensively addressed if an effective technology transfer and novel endeavors want to be ensured for chitosan and its derivatives.



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*Source: 865704-2011-10-04.xml*