# Sewage Sludge Amendment Combined with Green Manuring to a Coastal Mudflat Salt-Soil in Eastern China: Effects on Soil Physicochemical Properties and Maize Yield

Authors: Yanchao Bai; Wengang Zuo; Yiyun Yan; Chuanhui Gu; Yongxiang Guan; Lijuan Mei; Weijie Xue; Yuhua Shan; Ke Feng
Journal: International Journal of Agronomy
 (2017)

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## Abstract

Sewage sludge and green manure have become widely used organic amendments to croplands in many regions of the world. However, the amending effect of the combination of sewage sludge with green manuring in reclaimed coastal mudflat salt-soil has been unclear yet. This paper was one of earlier studies to investigate and evaluate the effects of sewage sludge amendment combined with green manuring on selected soil physicochemical properties of the mudflat soil in a rain-fed agroecosystem. The mudflat salt-soil was amended by one-time input of sewage sludge at the rates of 0, 30, 75, 150, and 300 t ha−1. After green manuring for three consecutive seasons, maize (Zea mays L.) was planted in 2013 and 2014. The results showed that SSA combined with green manuring decreased bulk density, pH, salinity, and exchangeable sodium percentage of the topsoil (0–20 cm soil layer) and increased aggregate stability, cation exchange capacity, and N and P concentration of the topsoil. As a result, the maize yield increased with the increase of SSA rates. Sewage sludge combined with green manuring can be applied in coastal mudflat salt-soil amendment, which provides an innovative way to create arable land resources and safe disposal of sewage sludge.


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## Body

## 1. Introduction

Soil salinity is a major obstacle in the potential utilization of land resources in salt-affected areas. Salt-affected soils are found in many parts of the world, and about 20% of the world’s cultivated land is salt-affected [1]. In the east of China, there were approximately 1.1~1.2 million ha salt-soil reclaimed from coastal mudflats in the past five decades [2, 3]. It is estimated that additional 2000 km2 mudflats along the Jiangsu coast will be reclaimed by 2020, and another reclamation of 4400 km2 mudflats located in the Yellow River Delta is under evaluation [4]. The newly reclaimed coastal mudflats, as typical salt-soil, have high salinity, high pH, and macronutrient deficiencies [5]. High salinity of mudflat salt-soil is driven by the strong evaporation demand of the air, shallow salt solution rise to the soil surface through soil capillary in the dry season [6]. In the area rich in freshwater resources, soil salinity reduction is often accomplished through freshwater irrigation [7]. However, faced with the scarcity of freshwater resources, organic matter amendment is an effective alternative to decrease soil salinity by forming soil aggregates and breaking capillary rise in salt-soil reclaimed from coastal mudflats in the eastern area of China [8, 9].Salt-soil generally exhibits poor aggregates due to low organic matter content. Previous studies indicated that soil aggregates can be improved by the application of organic materials such as poultry manures, green manures, and compost [10–13]. Barzegar et al. [14] found that the addition of plant residues to a salt-soil had a positive influence on the stability of soil aggregates and decreased the soil salinity and exchangeable sodium percentage (ESP).Sewage sludge generated in municipal wastewater treatment plants has caused serious environmental pollution and ecological safety concern. Safe disposal of sewage sludge is a challenge in China. Approximately 78.4 billion metric tons of sewage sludge was derived from more than 2600 wastewater treatment plants in China in 2015 [15]. Agricultural application of sewage sludge is an effective waste disposal and soil amendment technique that can improve the chemical, physical, and biological characteristics of soils [16–19]. Sewage sludge is rich in organic matter and other nutrients and can be used for mudflat salt-soil amendment [9, 20, 21].However, the quality and application rate of sewage sludge must be controlled, because it might bring various pollutants such as heavy metals, pathogens, and organic micropollutants into the environment and subsequently into the food chain [22, 23]. Thus, total amount of sewage sludge applied must be controlled in compliance with the China national standard for agricultural use of sewage sludge (GB/T 24600-2009) and on the premise of total amount of toxins elements from sewage sludge. Green manuring, on the other hands, plays an important role in soil quality and sustainability of agricultural systems and can be used as an alternative to sewage sludge to increase organic carbon to the soil [24]. Soil water-stable aggregates stability and soil organic matter can be improved by green manuring [25]. Green manuring was potential source for organic matter and nutrient [26, 27] and may provide economic and biological advantages over the traditional approach of soil incorporation [28]. Zubair et al. [29] found that green manuring in salt-affected soil increased soil organomineral interactions. Bruning et al. [30] also found the use of green manure can potentially increase crop productivity in a salt-affected soil and thus contribute to the sustainability of saline agricultural systems. Green manuring in mudflat salt-soil can be an effective way to minimize input of sewage sludge as the source of toxins elements.The past research mainly focused on the application of sewage sludge or green manuring in farmlands [31–34]. However, the application of sewage sludge combined with green manuring to amend mudflat salt-soils has received no attention. The impacts of applying sewage sludge alone to reclaimed mudflat soil on fertility development, growth of ryegrass,Sesbania, and maize and on heavy metals behaviors including their accumulation and fraction in soil and uptake by plants were investigated and evaluated in our previous studies [9, 20, 21, 35]. This study was one of pioneering studies to investigate and evaluate the sustaining effects of sewage sludge amendment combined with green manuring on the soil aggregates, organic carbon, salinity, pH, and N and P of mudflat salt-soil, as well as yield of maize (Zea mays L.) in mudflats salt-soil in a rain-fed agroecosystem.


## 2. Materials and Methods

### 2.1. Study Area and Experimental Materials

The experiment site was located in the Senmao Farm (E 121°23′23′′, N 32°20′03′′) of the Rudong County, Jiangsu Province, China. The land, as reclaimed from coastal mudflats in 2007, has been under amendment since 2011. The distance from the site to the Yellow Sea coastline is approximately 1.2 km. The predominant soil is silt loam and is typic salt-affected soil.The experiment region is characterized by subtropic humid monsoon climate with distinct seasons. Mean annual rainfall is 1041.1 mm with approximately 61% of annual rainfall occurring between June and August (from 2011 to 2014). Mean annual temperature is 15.7°C with an average 230.5 frost-free days and average sunshine duration of 5.2 h d−1. The cold, dry season is from October to March and the hot, wet season is between April and September.The experimental sewage sludge was collected from Sewage Treatment Plant of Rudong County in September, 2011. The quality of the sludge complied with the China national standard for agricultural use of sewage sludge (GB/T 24600-2009). Some chemical properties of mudflat salt-affected soil and sewage sludge were shown in Table1.Table 1
Basic chemical properties of the coastal mudflat salt-soil and sewage sludge used in this study.
Items
Mudflat soil
Sewage sludge
pH
9.02
6.32
Salinity (‰)
8.51
32.9
Organic carbon (g kg−1)
1.97
216.2
Total N (N g kg−1)
0.282
51.2
Total P (P g kg−1)
0.507
5.51
Alkaline N (N mg kg−1)
17.08
3440
Available P (P mg kg−1)
6.99
813


### 2.2. Experimental Design

The field experiment was conducted in randomized complete block design (RCB) with each plot of 4 m length and 4 m width. The organic carbon content in farm soil in this region was about 0.5%–3.0% of total soil weight. Therefore, 30, 75, 150, and 300 t ha−1 (metric tons per hectare) (about 0.5%, 0.9%, 1.6%, and 2.9% organic carbon of soil weight in the 0–20 cm soil layer, resp.) sewage sludge amendment (SSA) rates on a dried weight basis by one-time application were used. The soil without sludge amendment was the control soil. The plots were repeated three times. On experimental site, the sewage sludge was mixed uniformly with the soil of 20 cm layer by a rototiller in late October 2011. Ryegrass (Lolium perenne L.) andSesbania (Sesbania cannabina) were adopted as green manures due to their high-quality and salt-tolerance and successively planted and tilled from October 2011 to September 2012. The rain-fed ryegrass-maize rotation was carried out from October 2012 to September 2014. Maize (Zea mays L.) as a typical dryland and salt-susceptible crop was cultivated as a test crop. Maize was sowed two seeds per hole with average row spacing of 0.50 m and plant spacing of 0.25 m in early July 2013 and 2014 and harvested in late September 2013 and 2014. Thinning of maize seedlings was performed 20 days after sowing (DAS), leaving one seedling per hole. Weeds were hand-hoed two times at 20 and 48 DAS, respectively. During the experimental period, all plants were rain-fed, and no extra artificial irrigation was carried out.


### 2.3. Soil Analysis

Soil samples from each plot at depths of 0–20 and 20–40 cm were collected in quadruplicate in late September 2013 and 2014 at maize harvest stage. The soil samples were immediately transported to the laboratory. Soil bulk density was measured by the core method. Soil salinity was measured by the gravimetric method [36]. The size composition of soil water-stable aggregates was analyzed by wet-sieving method using soil aggregate analyzer (DIK-2001, Daiki, Japan). The K2Cr2O7 method was used for analysis of soil organic carbon. The pH of soil was measured in suspension of 1 : 5 (weight/volume) by pH meter (Model IQ150, Spectrum, USA). Alkaline N and available P were determined by alkaline hydrolysis diffusion and sodium bicarbonate (NaHCO3) extraction method, respectively. The cation exchange capacity (CEC) of soil was measured by using the ammonium acetate (NH4OAc) method after successively leached in sodium acetate (NaOAc) solution and alcohol. Exchangeable sodium content was analyzed to calculate exchangeable sodium percentage (ESP) and was extracted with ammonium acetate-ammonium hydroxide (NH4OAc-NH4OH) (pH 9) and measured by a flame spectrophotometer [36]. The all methods and procedures for soil chemical properties involved in this study were described in detail by the Soil and Agro-Chemistry Analysis [36].


### 2.4. Plant Analysis

Ten maize plants were sampled randomly from each plot for estimation of aerial part growth at their respective harvest seasons in late September 2013 and 2014. Maize grains were threshed using a miniature thresher for each replica, and yields of maize were determined by weighing the grains after dried at 105°C for 15 minutes and oven-dried at 80°C until constant weight was achieved.


### 2.5. Statistical Analysis

Analysis of variance (ANOVA) for the data obtained was conducted using SPSS 13.0 software (SPSS Inc., USA) with the model of RCB design. The differences between the treatments were detected by the least significant difference (LSD) method at the 0.05 level.


## 2.1. Study Area and Experimental Materials

The experiment site was located in the Senmao Farm (E 121°23′23′′, N 32°20′03′′) of the Rudong County, Jiangsu Province, China. The land, as reclaimed from coastal mudflats in 2007, has been under amendment since 2011. The distance from the site to the Yellow Sea coastline is approximately 1.2 km. The predominant soil is silt loam and is typic salt-affected soil.The experiment region is characterized by subtropic humid monsoon climate with distinct seasons. Mean annual rainfall is 1041.1 mm with approximately 61% of annual rainfall occurring between June and August (from 2011 to 2014). Mean annual temperature is 15.7°C with an average 230.5 frost-free days and average sunshine duration of 5.2 h d−1. The cold, dry season is from October to March and the hot, wet season is between April and September.The experimental sewage sludge was collected from Sewage Treatment Plant of Rudong County in September, 2011. The quality of the sludge complied with the China national standard for agricultural use of sewage sludge (GB/T 24600-2009). Some chemical properties of mudflat salt-affected soil and sewage sludge were shown in Table1.Table 1
Basic chemical properties of the coastal mudflat salt-soil and sewage sludge used in this study.
Items
Mudflat soil
Sewage sludge
pH
9.02
6.32
Salinity (‰)
8.51
32.9
Organic carbon (g kg−1)
1.97
216.2
Total N (N g kg−1)
0.282
51.2
Total P (P g kg−1)
0.507
5.51
Alkaline N (N mg kg−1)
17.08
3440
Available P (P mg kg−1)
6.99
813


## 2.2. Experimental Design

The field experiment was conducted in randomized complete block design (RCB) with each plot of 4 m length and 4 m width. The organic carbon content in farm soil in this region was about 0.5%–3.0% of total soil weight. Therefore, 30, 75, 150, and 300 t ha−1 (metric tons per hectare) (about 0.5%, 0.9%, 1.6%, and 2.9% organic carbon of soil weight in the 0–20 cm soil layer, resp.) sewage sludge amendment (SSA) rates on a dried weight basis by one-time application were used. The soil without sludge amendment was the control soil. The plots were repeated three times. On experimental site, the sewage sludge was mixed uniformly with the soil of 20 cm layer by a rototiller in late October 2011. Ryegrass (Lolium perenne L.) andSesbania (Sesbania cannabina) were adopted as green manures due to their high-quality and salt-tolerance and successively planted and tilled from October 2011 to September 2012. The rain-fed ryegrass-maize rotation was carried out from October 2012 to September 2014. Maize (Zea mays L.) as a typical dryland and salt-susceptible crop was cultivated as a test crop. Maize was sowed two seeds per hole with average row spacing of 0.50 m and plant spacing of 0.25 m in early July 2013 and 2014 and harvested in late September 2013 and 2014. Thinning of maize seedlings was performed 20 days after sowing (DAS), leaving one seedling per hole. Weeds were hand-hoed two times at 20 and 48 DAS, respectively. During the experimental period, all plants were rain-fed, and no extra artificial irrigation was carried out.


## 2.3. Soil Analysis

Soil samples from each plot at depths of 0–20 and 20–40 cm were collected in quadruplicate in late September 2013 and 2014 at maize harvest stage. The soil samples were immediately transported to the laboratory. Soil bulk density was measured by the core method. Soil salinity was measured by the gravimetric method [36]. The size composition of soil water-stable aggregates was analyzed by wet-sieving method using soil aggregate analyzer (DIK-2001, Daiki, Japan). The K2Cr2O7 method was used for analysis of soil organic carbon. The pH of soil was measured in suspension of 1 : 5 (weight/volume) by pH meter (Model IQ150, Spectrum, USA). Alkaline N and available P were determined by alkaline hydrolysis diffusion and sodium bicarbonate (NaHCO3) extraction method, respectively. The cation exchange capacity (CEC) of soil was measured by using the ammonium acetate (NH4OAc) method after successively leached in sodium acetate (NaOAc) solution and alcohol. Exchangeable sodium content was analyzed to calculate exchangeable sodium percentage (ESP) and was extracted with ammonium acetate-ammonium hydroxide (NH4OAc-NH4OH) (pH 9) and measured by a flame spectrophotometer [36]. The all methods and procedures for soil chemical properties involved in this study were described in detail by the Soil and Agro-Chemistry Analysis [36].


## 2.4. Plant Analysis

Ten maize plants were sampled randomly from each plot for estimation of aerial part growth at their respective harvest seasons in late September 2013 and 2014. Maize grains were threshed using a miniature thresher for each replica, and yields of maize were determined by weighing the grains after dried at 105°C for 15 minutes and oven-dried at 80°C until constant weight was achieved.


## 2.5. Statistical Analysis

Analysis of variance (ANOVA) for the data obtained was conducted using SPSS 13.0 software (SPSS Inc., USA) with the model of RCB design. The differences between the treatments were detected by the least significant difference (LSD) method at the 0.05 level.


## 3. Results

### 3.1. Size Composition of Soil Water-Stable Aggregates and Bulk Density

Table2 presents the test results of the water-stable aggregates size composition in the soil samples collected in September 2013 and 2014. Under the influence of different sewage sludge amendment combined with green manuring, the percentage of aggregates of different size classes significantly varied in the topsoil (0–20 cm) and subsurface (20–40 cm) layers. The percentage of >0.25 mm and 0.106–0.25 mm water-stable aggregates in the topsoil increased with increasing SSA rates, whereas no significant changes (p>0.05) were found in the percentage of water-stable aggregates of the subsurface soil. The percentage of >0.25 mm water-stable aggregates in the topsoil increased by 7.5–25.1%, 29.7–96.1%, 105.5–121.6%, and 143.5–247.7% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil. There were sharp increases in the percentage of >0.25 mm water-stable aggregates in the topsoil at all SSA rates in September 2014, compared to those in 2013.Table 2
Effects of sewage sludge amendment (SSA) combined with green manuring on the size composition of soil water-stable aggregates at 0–20 cm and 20–40 cm depths of coastal mudflat salt-soil.
Year
SSA (t ha−1)
Water-stable aggregates (%)
0–20 cm depth
20–40 cm depth
>0.25 mm
0.106–0.25 mm
<0.106 mm
>0.25 mm
0.106–0.25 mm
<0.106 mm
2013
0
3.33 ± 1.42c
0.98 ± 0.16b
95.71 ± 1.92a
0.61 ± 0.35a
0.59 ± 0.06a
98.80 ± 0.29a
30
3.58 ± 1.50bc
1.33 ± 0.60b
95.11 ± 3.09a
2.98 ± 2.81a
0.77 ± 0.32a
96.25 ± 3.13a
75
4.32 ± 2.35bc
1.09 ± 0.45b
94.60 ± 2.80a
1.02 ± 0.61a
0.52 ± 0.03a
98.46 ± 0.58a
150
7.38 ± 2.58b
1.75 ± 0.47b
90.87 ± 3.04ab
2.31 ± 0.96a
0.81 ± 0.19a
96.88 ± 1.15a
300
11.58 ± 2.69a
2.97 ± 0.98a
85.46 ± 4.17b
1.20 ± 0.10a
0.50 ± 0.06a
98.30 ± 0.16a
F-value
7.77∗∗
5.59∗
5.80∗
1.44ns
1.07ns
1.05ns
2014
0
13.00 ± 2.17b
3.18 ± 0.95b
83.83 ± 1.46a
0.99 ± 0.19a
0.50 ± 0.06a
98.51 ± 0.13a
30
16.26 ± 7.23b
3.41 ± 0.38b
80.34 ± 7.57ab
0.68 ± 0.45a
0.59 ± 0.06a
98.73 ± 0.38a
75
25.49 ± 7.58a
5.11 ± 2.08ab
69.40 ± 9.62bc
1.97 ± 1.95a
0.77 ± 0.32a
97.26 ± 2.27a
150
26.72 ± 2.43a
5.12 ± 0.46ab
68.16 ± 2.41c
2.22 ± 1.09a
0.72 ± 0.32a
97.06 ± 1.41a
300
31.65 ± 2.93a
6.84 ± 4.45a
61.51 ± 7.27c
1.45 ± 0.26a
0.61 ± 0.10a
97.94 ± 0.35a
F-value
6.98∗∗
5.72∗
6.04∗∗
0.78ns
0.541ns
0.73ns
Values are mean ± SD of three replicates. Different letters in each column indicate significant difference atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗<0.05; p∗∗<0.01; pns>0.05.The sewage sludge amendment combined with green manuring significantly decreased bulk density in the salt-soil, and soil bulk density decreased with increasing SSA rates (Figure1). The similar trend was found in 2013 and 2014. The soil bulk density decreased by 7.4%, 12.2%, 12.3%, and 18.5% at 30, 75, 150, and 300 t ha−1 SSA rates in 2014, respectively, compared to the control soil.Figure 1
Effects of sewage sludge amendment (SSA) combined with green manuring on bulk density of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01; pns>0.05.


### 3.2. Soil Salinity, pH, and ESP

As shown in Table3, the SSA combined with green manuring significantly decreased salinity of the topsoil, whereas no significant changes (p>0.05) were found in salinity of the subsurface soil. The salinity of topsoil decreased by 26.5–37.9%, 29.9–59.8%, 44.7–66.6%, and 56.7–73.1% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil.Table 3
Effects of sewage sludge amendment (SSA) combined with green manuring on salinity of coastal mudflat salt-soil.
Year
SSA (t ha−1)
Salinity (‰)
0–20 cm depth
20–40 cm depth
2013
0
7.67 ± 0.11a
9.40 ± 0.57a
30
4.76 ± 0.15b
8.38 ± 1.01a
75
3.08 ± 0.05c
8.48 ± 1.06a
150
2.56 ± 0.25d
8.25 ± 1.30a
300
2.06 ± 0.24e
7.97 ± 0.82a
F-value
491.75∗∗
0.90ns
2014
0
6.33 ± 2.13a
9.34 ± 0.27a
30
4.65 ± 0.63b
8.14 ± 0.63a
75
4.44 ± 0.07bc
8.57 ± 1.25a
150
3.50 ± 0.66cd
8.27 ± 1.27a
300
2.74 ± 0.82d
7.75 ± 0.51a
F-value
17.96∗∗
1.37ns
Values are mean ± SD of three replicates. Different letters in each column indicate significant difference atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗<0.05; p∗∗<0.01; pns>0.05.The sewage sludge amendment combined with green manuring significantly decreased pH in coastal mudflat salt-soil, and pH decreased with increasing SSA rates in 2013 and 2014 (Figure2(a)). The pH decreased by 0.32–0.47, 0.38–0.79, 0.63–1.29, and 1.13–1.65 pH-unit at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil. The ESP in mudflat salt-soil also decreased with increasing SSA rates in 2013 and 2014 (Figure 2(b)). The ESP decreased by 14.0–22.7%, 32.0–38.7%, 43.2–54.0%, and 60.2–69.8% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil.Figure 2
Effects of sewage sludge amendment (SSA) combined with green manuring on pH (a) and ESP (b) of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01. pH, hydrogen ion concentration; ESP, exchangeable sodium percentage.
(a)
(b)


### 3.3. Soil OC, CEC, and Nutrient Content

As shown in Figure3(a), the sewage sludge amendment combined with green manuring significantly increased OC content in mudflat salt-soil, and the OC content increased with increasing SSA rates in 2013 and 2014. The SSA combined with green manuring increased OC concentration by 97.5–122.2%, 167.9–205.7%, 320.2–345.2%, and 352.8–427.8% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared with the control soil. The sewage sludge amendment combined with green manuring significantly increased CEC in mudflat salt-soil, and CEC increased with increasing SSA rates in 2013 and 2014 (Figure 3(b)). The CEC increased by 1.1–15.0%, 17.0–38.9%, 46.3–50.7%, and 65.6–76.4% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil.Figure 3
Effects of sewage sludge amendment (SSA) combined with green manuring on organic carbon (a) and CEC (b) of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01. CEC, cation exchange capacity.
(a)
(b)The sewage sludge amendment combined with green manuring significantly increased total N and P, alkaline N, and available P in mudflat salt-soil (Figure4). The concentrations of total N and P, alkaline N, and available P in mudflat salt-soil increased with increasing SSA rates, and the similar trend was found in 2013 and 2014. The total N and P increased by 18.0%, 129.0%, 193.0%, and 311.0% and 62.7%, 71.2%, 232.2%, and 291.7% at 30, 75, 150, and 300 t ha−1 SSA rates in 2014, respectively, compared to the control soil. The increments of alkaline N and available P in mudflat salt-soil in 2014 were 209.8%, 203.4%, 402.8%, and 505.8% and 184.9%, 190.1%, 239.3%, and 309.6% at 30, 75, 150, and 300 t ha−1 SSA rates, respectively, compared to the control soil.Figure 4
Effects of sewage sludge amendment (SSA) combined with green manuring on the concentration of total N (a), total P (b), alkaline N (c), and available P (d) of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01. N, nitrogen; P, phosphorus.
(a)
(b)
(c)
(d)


### 3.4. Maize Yields

The sewage sludge amendment combined with green manuring significantly increased yields of maize grown in mudflat salt-soil (Figure5). The maize yield increased by 225.0%, 471.1%, 546.1%, and 648.7% at 30, 75, 150, and 300 t ha−1 SSA rates in 2014, respectively, compared to the control soil. The similar trend was found in 2013, and the yields of maize at ≥ 75 t ha−1 SSA rates were significantly higher than that in the control soil.Figure 5
Effects of sewage sludge amendment (SSA) combined with green manuring on yield of maize grown in the mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01.


## 3.1. Size Composition of Soil Water-Stable Aggregates and Bulk Density

Table2 presents the test results of the water-stable aggregates size composition in the soil samples collected in September 2013 and 2014. Under the influence of different sewage sludge amendment combined with green manuring, the percentage of aggregates of different size classes significantly varied in the topsoil (0–20 cm) and subsurface (20–40 cm) layers. The percentage of >0.25 mm and 0.106–0.25 mm water-stable aggregates in the topsoil increased with increasing SSA rates, whereas no significant changes (p>0.05) were found in the percentage of water-stable aggregates of the subsurface soil. The percentage of >0.25 mm water-stable aggregates in the topsoil increased by 7.5–25.1%, 29.7–96.1%, 105.5–121.6%, and 143.5–247.7% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil. There were sharp increases in the percentage of >0.25 mm water-stable aggregates in the topsoil at all SSA rates in September 2014, compared to those in 2013.Table 2
Effects of sewage sludge amendment (SSA) combined with green manuring on the size composition of soil water-stable aggregates at 0–20 cm and 20–40 cm depths of coastal mudflat salt-soil.
Year
SSA (t ha−1)
Water-stable aggregates (%)
0–20 cm depth
20–40 cm depth
>0.25 mm
0.106–0.25 mm
<0.106 mm
>0.25 mm
0.106–0.25 mm
<0.106 mm
2013
0
3.33 ± 1.42c
0.98 ± 0.16b
95.71 ± 1.92a
0.61 ± 0.35a
0.59 ± 0.06a
98.80 ± 0.29a
30
3.58 ± 1.50bc
1.33 ± 0.60b
95.11 ± 3.09a
2.98 ± 2.81a
0.77 ± 0.32a
96.25 ± 3.13a
75
4.32 ± 2.35bc
1.09 ± 0.45b
94.60 ± 2.80a
1.02 ± 0.61a
0.52 ± 0.03a
98.46 ± 0.58a
150
7.38 ± 2.58b
1.75 ± 0.47b
90.87 ± 3.04ab
2.31 ± 0.96a
0.81 ± 0.19a
96.88 ± 1.15a
300
11.58 ± 2.69a
2.97 ± 0.98a
85.46 ± 4.17b
1.20 ± 0.10a
0.50 ± 0.06a
98.30 ± 0.16a
F-value
7.77∗∗
5.59∗
5.80∗
1.44ns
1.07ns
1.05ns
2014
0
13.00 ± 2.17b
3.18 ± 0.95b
83.83 ± 1.46a
0.99 ± 0.19a
0.50 ± 0.06a
98.51 ± 0.13a
30
16.26 ± 7.23b
3.41 ± 0.38b
80.34 ± 7.57ab
0.68 ± 0.45a
0.59 ± 0.06a
98.73 ± 0.38a
75
25.49 ± 7.58a
5.11 ± 2.08ab
69.40 ± 9.62bc
1.97 ± 1.95a
0.77 ± 0.32a
97.26 ± 2.27a
150
26.72 ± 2.43a
5.12 ± 0.46ab
68.16 ± 2.41c
2.22 ± 1.09a
0.72 ± 0.32a
97.06 ± 1.41a
300
31.65 ± 2.93a
6.84 ± 4.45a
61.51 ± 7.27c
1.45 ± 0.26a
0.61 ± 0.10a
97.94 ± 0.35a
F-value
6.98∗∗
5.72∗
6.04∗∗
0.78ns
0.541ns
0.73ns
Values are mean ± SD of three replicates. Different letters in each column indicate significant difference atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗<0.05; p∗∗<0.01; pns>0.05.The sewage sludge amendment combined with green manuring significantly decreased bulk density in the salt-soil, and soil bulk density decreased with increasing SSA rates (Figure1). The similar trend was found in 2013 and 2014. The soil bulk density decreased by 7.4%, 12.2%, 12.3%, and 18.5% at 30, 75, 150, and 300 t ha−1 SSA rates in 2014, respectively, compared to the control soil.Figure 1
Effects of sewage sludge amendment (SSA) combined with green manuring on bulk density of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01; pns>0.05.


## 3.2. Soil Salinity, pH, and ESP

As shown in Table3, the SSA combined with green manuring significantly decreased salinity of the topsoil, whereas no significant changes (p>0.05) were found in salinity of the subsurface soil. The salinity of topsoil decreased by 26.5–37.9%, 29.9–59.8%, 44.7–66.6%, and 56.7–73.1% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil.Table 3
Effects of sewage sludge amendment (SSA) combined with green manuring on salinity of coastal mudflat salt-soil.
Year
SSA (t ha−1)
Salinity (‰)
0–20 cm depth
20–40 cm depth
2013
0
7.67 ± 0.11a
9.40 ± 0.57a
30
4.76 ± 0.15b
8.38 ± 1.01a
75
3.08 ± 0.05c
8.48 ± 1.06a
150
2.56 ± 0.25d
8.25 ± 1.30a
300
2.06 ± 0.24e
7.97 ± 0.82a
F-value
491.75∗∗
0.90ns
2014
0
6.33 ± 2.13a
9.34 ± 0.27a
30
4.65 ± 0.63b
8.14 ± 0.63a
75
4.44 ± 0.07bc
8.57 ± 1.25a
150
3.50 ± 0.66cd
8.27 ± 1.27a
300
2.74 ± 0.82d
7.75 ± 0.51a
F-value
17.96∗∗
1.37ns
Values are mean ± SD of three replicates. Different letters in each column indicate significant difference atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗<0.05; p∗∗<0.01; pns>0.05.The sewage sludge amendment combined with green manuring significantly decreased pH in coastal mudflat salt-soil, and pH decreased with increasing SSA rates in 2013 and 2014 (Figure2(a)). The pH decreased by 0.32–0.47, 0.38–0.79, 0.63–1.29, and 1.13–1.65 pH-unit at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil. The ESP in mudflat salt-soil also decreased with increasing SSA rates in 2013 and 2014 (Figure 2(b)). The ESP decreased by 14.0–22.7%, 32.0–38.7%, 43.2–54.0%, and 60.2–69.8% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil.Figure 2
Effects of sewage sludge amendment (SSA) combined with green manuring on pH (a) and ESP (b) of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01. pH, hydrogen ion concentration; ESP, exchangeable sodium percentage.
(a)
(b)


## 3.3. Soil OC, CEC, and Nutrient Content

As shown in Figure3(a), the sewage sludge amendment combined with green manuring significantly increased OC content in mudflat salt-soil, and the OC content increased with increasing SSA rates in 2013 and 2014. The SSA combined with green manuring increased OC concentration by 97.5–122.2%, 167.9–205.7%, 320.2–345.2%, and 352.8–427.8% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared with the control soil. The sewage sludge amendment combined with green manuring significantly increased CEC in mudflat salt-soil, and CEC increased with increasing SSA rates in 2013 and 2014 (Figure 3(b)). The CEC increased by 1.1–15.0%, 17.0–38.9%, 46.3–50.7%, and 65.6–76.4% at 30, 75, 150, and 300 t ha−1 SSA rates in 2013 and 2014, respectively, compared to the control soil.Figure 3
Effects of sewage sludge amendment (SSA) combined with green manuring on organic carbon (a) and CEC (b) of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01. CEC, cation exchange capacity.
(a)
(b)The sewage sludge amendment combined with green manuring significantly increased total N and P, alkaline N, and available P in mudflat salt-soil (Figure4). The concentrations of total N and P, alkaline N, and available P in mudflat salt-soil increased with increasing SSA rates, and the similar trend was found in 2013 and 2014. The total N and P increased by 18.0%, 129.0%, 193.0%, and 311.0% and 62.7%, 71.2%, 232.2%, and 291.7% at 30, 75, 150, and 300 t ha−1 SSA rates in 2014, respectively, compared to the control soil. The increments of alkaline N and available P in mudflat salt-soil in 2014 were 209.8%, 203.4%, 402.8%, and 505.8% and 184.9%, 190.1%, 239.3%, and 309.6% at 30, 75, 150, and 300 t ha−1 SSA rates, respectively, compared to the control soil.Figure 4
Effects of sewage sludge amendment (SSA) combined with green manuring on the concentration of total N (a), total P (b), alkaline N (c), and available P (d) of coastal mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01. N, nitrogen; P, phosphorus.
(a)
(b)
(c)
(d)


## 3.4. Maize Yields

The sewage sludge amendment combined with green manuring significantly increased yields of maize grown in mudflat salt-soil (Figure5). The maize yield increased by 225.0%, 471.1%, 546.1%, and 648.7% at 30, 75, 150, and 300 t ha−1 SSA rates in 2014, respectively, compared to the control soil. The similar trend was found in 2013, and the yields of maize at ≥ 75 t ha−1 SSA rates were significantly higher than that in the control soil.Figure 5
Effects of sewage sludge amendment (SSA) combined with green manuring on yield of maize grown in the mudflat salt-soil. Vertical bars indicate standard deviations of the means. Columns with different letters show significant difference between sewage sludge amendment rates atp<0.05 by LSD’s multiple range test. Values of F are the F test in ANOVA; p∗∗<0.01.


## 4. Discussion

The sewage sludge amendment combined with green manuring improved physical properties of topsoil in mudflat salt-soil by increasing the percentage of >0.25 mm and 0.106–0.25 mm water-stable aggregates and by decreasing bulk density in 0–20 cm of the salt-soil layer. The improvement in these physical properties was due to increase in organic carbon content of salt-soil. Soil aggregates are formed from the combination of soil organic matter and soil mineral particles [37]. A significant positive correlation was found between soil organic carbon and the percentage of >0.25 mm and 0.106–0.25 mm water-stable aggregates (p<0.01) (Figure 6(a)). Release of carbohydrates and organic carbon from biodegradation of OM-rich sludge-amended soil may increase aggregate stability percentage, as it can complex soil organic matter and soil mineral particles to soil aggregate [38, 39]. The result in the reduction in bulk density by the amendments was not only due to the addition of less dense organic material, but also due to the increase in soil aggregate stability [40] (Figure 6(b)). Previous studies have shown that the application of sewage sludge increased organic carbon in saline and salinized soil [41, 42], subsequently increased aggregate stability in saline-sodic and salinized soil [42, 43], and decreased bulk density in salt-soil and salinized soil [9, 42].Figure 6
Relationships between percentage of >0.25 mm soil water-stable aggregate (SA) and organic carbon (a), bulk density (b), salinity (c), and ESP (d) in the mudflat salt-soil.
(a)
(b)
(c)
(d)Topsoil salinity in mudflat salt-soil decreased as a result of increasing soil aggregate stability and decreasing bulk density. Increase in noncapillary porosity by the increment of soil aggregates stability percentage [8] breaks capillary rise [6] that translocates saline groundwater to topsoil. Guo and Liu [5] also reported that the high salinity in salt-soil is caused by capillary rise that brings saline groundwater to topsoil. In this study, the SSA significantly decreased soil salinity in 0–20 cm layer, whereas there were no significant changes (p>0.05) in 20–40 cm layer. On the contrary, water-stable aggregates percentage significantly increased in 0–20 cm layer, whereas there are no significant changes (p>0.05) in 20–40 cm layer. Statistical analysis showed that a significant negative correlation was found between soil salinity and soil aggregate stability percentage in topsoil and subsurface soil (p<0.05) (Figure 6(c)). The increase in aggregate stability percentage in topsoil acted as a barrier for upward movement of saline groundwater. Thus, soil salinity in 0–20 cm layer was lower than in 20–40 cm layer.The ESP values in mudflat salt-soil decreased with increasing SSA rates. Similar effect was also observed by García-Orenes et al. [42] after the biosolids application to a salinized soil. The decrease in ESP values might be associated with an increase in infiltration rate that enhances soil washing and thus sodium leachability [44]. Moreover, sodium as a micronutrient could be taken up into plant tissue, specially after the three consecutive seasons of green manuring. In addition, a slight dilution effect can be caused by high doses of sewage sludge application and green manure incorporation. In the present study, statistical analysis also showed a significant negative correlation between soil ESP values and soil aggregate stability percentage in topsoil (p<0.01) (Figure 6(d)). The similar negative effects of ESP on soil aggregation were found by other studies [45–47]. This might be attributed to that sodium causes swelling and dispersion of soil mineral particles and slaking of unstable aggregates [45, 48]. In addition, high ESP value can reduce microbial biomass and activity, which may also contribute to the decrease in aggregation [49].The pH in mudflat salt-soil decreased with increasing SSA rates, which was similar to our previous studies [9, 20, 21]. Other authors have also found lowering of pH with SSA [50–52]. Intense mineralization of the labile OM pool of sludge treated-soils [53] lowers pH by releasing humic acid from biodegradation of OM-rich sewage sludge and green manure [50]. In addition, a slight dilution effect could be caused by lower-pH sewage sludge application, especially in treatments with high doses of application.The sewage sludge amendment combined with green manuring improved salt-soil fertility by increasing CEC and concentration of N and P in mudflat salt-soil. Our previous studies also supported the results [9, 20, 21]. In farmland, soil fertility improvement by SSA has also been reported widely [50, 51, 54]. Soil N and P content increased in the mudflat salt-soil amended with sewage sludge due to higher levels of these nutrients in the sewage sludge. Moreover, green manuring, such as Sesbania as a legume, may supply N through the N fixation process, thereby increasing N for soil fertility supply [55, 56]. The increase in CEC of mudflat soil is probably due to increasing soil OM content [57, 58].Improved physicochemical properties in the mudflat salt-soil enhanced the yields of maize (Zea mays L.) in the mudflat salt-soil. Our previous studies observed the SSA increased biomass of ryegrass (Lolium perenne L.),Sesbania (Sesbania cannabina), and maize (Zea mays L.) [20, 35, 59]. Other studies in farmland also reported that the SSA increased the yields of maize (Zea mays L.) [60], rice (Oryza sativa L.) [61], durum wheat (Triticum durum Desf.) [62], and Spinach (Spinacia oleracea L.) [63]. The yield increase might be the consequence of improved salt-soil physicochemical properties and enriched high-quality OM, N, P, and other nutrients from sewage sludge and green manures.


## 5. Conclusions

The sewage sludge amendment combined with green manuring increased organic carbon in mudflat salt-soil, which improved the physical properties of mudflat salt-soil by increasing aggregate stability of the 0–20 cm soil layer and decreasing topsoil bulk density. The increase in water-stable aggregate percentage in topsoil acted as a barrier for upward movement of saline groundwater. As a result, soil salinity of the topsoil decreased. The ESP values in mudflat salt-soil decreased with increasing sewage sludge amendment rates. The percentage of water-stable aggregate in mudflat salt-soil showed significantly negative correlation with soil salinity and ESP in mudflat salt-soil (p<0.01). In addition, the sewage sludge amendment combined with green manuring decreased soil pH and improved soil fertility by increasing CEC and concentration of N and P in mudflat salt-soil. The improved physicochemical properties subsequently enhanced the yields of maize (Zea mays L.) in the mudflat salt-soil.



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*Source: 8526598-2017-09-28.xml*