# Evolutionary Conservation and Diversification of the Translation Initiation Apparatus in Trypanosomatids

Authors: Alexandra Zinoviev; Michal Shapira
Journal: Comparative and Functional Genomics
 (2012)

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## Abstract

Trypanosomatids are ancient eukaryotic parasites that migrate between insect vectors and mammalian hosts, causing a range of diseases in humans and domestic animals. Trypanosomatids feature a multitude of unusual molecular features, including polycistronic transcription and subsequent processing bytrans-splicing and polyadenylation. Regulation of protein coding genes is posttranscriptional and thus, translation regulation is fundamental for activating the developmental program of gene expression. The spliced-leader RNA is attached to all mRNAs. It contains an unusual hypermethylated cap-4 structure in its 5′ end. The cap-binding complex, eIF4F, has gone through evolutionary changes in accordance with the requirement to bind cap-4. The eIF4F components in trypanosomatids are highly diverged from their orthologs in higher eukaryotes, and their potential functions are discussed. The cap-binding activity in all eukaryotes is a target for regulation and plays a similar role in trypanosomatids. Recent studies revealed a novel eIF4E-interacting protein, involved in directing stage-specific and stress-induced translation pathways. Translation regulation during stress also follows unusual regulatory cues, as the increased translation of Hsp83 following heat stress is driven by a defined element in the 3′ UTR, unlike higher eukaryotes. Overall, the environmental switches experienced by trypanosomatids during their life cycle seem to affect their translational machinery in unique ways.


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## Body

## 1. Translation Initiation in Eukaryotes

Translation is a complex process that is controlled by a large number of proteins and factors, allowing the cell to generate a rapid response to external and internal signals. The initiation step of protein synthesis is predominantly viewed as the limiting step of this process, thus serving as a target for a multitude of regulatory cues.During cap-dependent translation initiation, a preassembled 43S preinitiation complex (PIC) is targeted to the 5′ end of the mRNA through the cap-binding complex, eIF4F. The PIC subunits are comprised of the Met-tRNA along with eIF1, eIF1A, eIF2, and the multisubunit eIF3, which is responsible for recruiting the small ribosomal subunit (reviewed in [1, 2]). The eIF4F complex consists of the cap-binding protein, eIF4E, an RNA helicase, eIF4A, and the scaffold protein, eIF4G. The mammalian eIF4G holds together the eIF4F subunits and links them to the 43S PIC by interacting with eIF4E, eIF4A, and eIF3 [3, 4]; it is also responsible for the transient circularization of translating mRNAs, by interacting with the poly(A) binding protein (PABP) that is bound at their 3′ ends (reviewed in [5, 6]). The initiation complex scans the 5′ untranslated region (5′ UTR) until it reaches the initiator AUG codon, where the 60S ribosomal subunit joins to form the mature 80S ribosome. Two factors, eIF5B [7] and eIF6 [8], participate in this step, releasing most of the initiation factors. The scanning process is assisted by helicases which are associated with the initiation complex, with eIF4A serving as the main candidate, although it is not necessarily the only factor involved in this process [9, 10]. The activity of eIF4A is enhanced by an additional factor, eIF4B, which was shown to be essential for the helicase activity [11]. Once the ribosome advances along the mRNA and vacates the AUG start codon, a new initiation complex can be formed on the 5′ cap, leading to the formation of a polyribosome. Actively translating mRNAs are usually associated with the polysomal fraction of disrupted cells.Exposure to a variety of environmental, nutritional, or virus induced stresses, causes a global inhibition of cap-dependent translation. However, certain mRNAs continue to be translated in a cap-independent manner, by which the small ribosomal subunit is targeted to a position that is adjacent to the initiator AUG, with the aid of a highly structured RNA element in the 5′ UTR. This element is denoted the internal ribosome entry site (IRES) [12]. The IRES assigns a functional secondary structure, usually with the aid of trans-activating factors, such as the poly-pyrimidine-tract-binding (PTB) protein, ITAF45 or the La antigen [13]. IRES elements were also reported for endogenous mRNAs [12, 14–16], however, they do not account for all cases of cap-independent translation, and other, less understood pathways, are probably also used by eukaryotic cells [17, 18].Translation initiation is controlled by complex signaling networks, which integrate the internal and external conditions of living cells. It was reported that the arrest in cap-dependent translation observed under stress conditions is coordinated with the dephosphorylation and activation of the 4E-binding protein (4E-BP) The latter competes with eIF4G on binding to eIF4E, thus excluding assembly of the cap-binding complex [19–21]. The phosphorylation status of 4E-BP is mediated by the TOR-based complexes [22] that link between metabolism, protein synthesis and cell cycle [23, 24], which are controlled by a variety of cellular pathways [25]. While in most cases, blocking the dephosphorylation of 4E-BP led to inhibition of protein synthesis, exceptional cases have been reported where protein synthesis was not interrupted despite the dephosphorylation of 4E-BP, which was induced by specific mTOR inhibitors [26].Another way by which translation can be globally stopped is through phosphorylation of eIF2α, which forms a ternary complex with Met-tRNA-GTP. Following the hydrolysis of GTP, activity of eIF2α is regenerated by the eIF2B-mediated exchange of GDP with GTP. It is commonly observed that phosphorylation of eIF2α at Ser 51 blocks this exchange, resulting in a global translational arrest [27].Translation can be regulated also at a gene-specific level, without affecting the overall translation capacity of the cell. In many cases, a regulatory protein that is recruited by elements in the 3′ UTR binds eIF4E, in a manner that blocks the access to eIF4G and prevents assembly of the eIF4F complex. Such a pattern of regulation is observed during the development ofDrosophila embryos, in which specific proteins and mRNAs are distributed unevenly along the body axis. For example, Maskin interacts with the eIF4E only on RNAs that contain a cytoplasmic polyadenylation element (CPE); disruption of the eIF4E-eIF4G complex by this protein is therefore specific to mRNAs that contain a CPE in their 3′ UTR (reviewed in [21]).Exposure to extreme conditions such as temperature and pH alterations, osmotic switches, UV irradiation, nutrient starvation and oxidative stress is hazardous to all living cells, and is combatted by activation of a complex chaperone network. Extreme temperatures cause cellular damage at multiple levels, accompanied by a global arrest in synthesis of most cellular proteins, except for heat shock proteins (HSPs). In cases where the cell cannot overcome the inflicted damage, apoptosis may be induced [16]. The increased translation of HSPs in higher eukaryotes is controlled by the 5′ UTR, as shown for both HSP70 [28, 29] and HSP90 [30]. HSP90 and some variants of HSP70 are expressed at ambient temperatures, but their translation increases dramatically during heat shock. Despite extensive efforts, no functional motifs that could drive preferential translation at elevated temperatures were yet identified. It was also suggested that under normal temperatures, translation of Hsp90 occurs by a typical cap-dependent scanning mechanism, whereas during heat shock, translation shifts to a cap-independent mode.


## 2. Trypanosomatid Organisms

Trypanosomatids are unicellular, diploid protists from the Kinetoplastidæ order, which migrate between insect vectors and mammalian hosts, causing a variety of parasitic diseases in humans and their domestic animals.Leishmania parasites reside in the alimentary tract of female sandflies as extracellular flagellated promastigotes [31, 32]. Upon transmission to mammalian hosts, they differentiate into obligatory intracellular amastigotes within macrophages and dendritic cells [33, 34], causing a range of symptoms, that depend on the infecting species. Trypanosoma cruzi is the causative agent of Chagas disease in South America, and T. bruceispp. cause the African sleeping sickness. Altogether, trypanosomatid-borne diseases cause a great health threat and economical drawbacks for native populations living in endemic countries, as well as being a major problem for travelers visiting these regions. The divergence of most trypanosomatid species occurred very early in evolution, before the emergence of both vectors and hosts [35]. Thus, unique molecular properties are common in this family as compared to higher eukaryotes.Protein coding genes in trypanosomatids are arranged as large, unidirectional transcription units, which are transcribed polycistronically. The resulting pre-mRNAs are matured bytrans-splicing and polyadenylation into mono-cistronic mRNAs (reviewed in [36, 37]). Conventional RNA polymerase II promoters were not identified to date, and there is yet no evidence for the occurrence of regulated transcription activation processes for protein coding genes. Thus, mRNA processing, stability and translation serve as key mechanisms that direct differential program of gene expression throughout the pirasite life cycle (reviewed in [38]). During trans-splicing, a conserved mini-exon of 39 nucleotides is donated by the spliced leader RNA (SL RNA) to the 5′ end of all mRNAs. This mini-exon provides the 5′ cap structure, which in trypanosomatids is heavily methylated on the first four nucleotides and is thus denoted cap-4 [39]. In addition to the consensus m7GTP, cap-4 consists of 2′-O ribose methylations on the first four nucleotides of the SL RNA and two base methylations on the first adenosine and fourth uridine (Figure 1). These base methylations are unique to trypanosomatids, and are not known in any other group of eukaryotes [40]. Substitutions of individual nucleotides in the cap-4 structure diminished the ability of the mutated SL RNA to be utilized in trans-splicing reactions [41] and thus it was not possible to evaluate the exact role of ribose methylation in translation. The enzymes that promote the 2′-O-methylations on the ribose moieties of the cap-4 nucleotides were identified [42, 43], and the effect of their elimination was tested. Single and double knock-out mutants of genes encoding one or two of the enzymes responsible for ribose 2′-O-methylation at position 1 (TbMTr1), 2 (TbMTr2), 3 and 4 (TbMTr3) were examined. Preventing the ribose methylations at positions 3 and 4, but not at position 1, reduced the translation rates; a further exacerbation was observed by additional loss of the methylation at position 2. Knock-out of TbMTr1 alone did not cause an inhibitory effect on translation, however, depletion of TbMTr2 or TbMTr3 on TbMTr1−/− background did not yield viable parasites [44, 45]. Therefore, it was proposed that only a minimal level of mRNA cap ribose methylation is essential for trypanosome viability. The role of cap-1 modification was shown to be related to the SL RNA biogenesis, as formerly shown for Leptomonas collosoma mutants [37].Figure 1
Trypanosomatid cap-4 structure, based on Bangs et al., 1992 [40].


## 3. eIF4E Isoforms of Trypanosomatids

Trypanosomatid genome annotation combined with a functional analysis approach, paved the way for studies of the translation apparatus in these organisms. Some of the factors were subject to a thorough biochemical and cellular analysis, whereas others were only identified based on sequence homology. Homology modeling of the four LeishIF4E paralogs suggested that the structure of the cap-binding pocket was conserved and maintained the basic features observed for the yeast and mammalian eIF4E [46]. It is of interest to note that the association constants for complexes of LeishIF4E homologues with m7GTP as well as with cap-4 were two orders of magnitude lower than those of the mouse protein [46]. This could be related to the evolutionary changes that occurred in the parasite proteins to promote their interaction with cap-4. However, this has yet to be proven.eIF4E is the eukaryote 5′ cap-binding translation initiation factor; its association with eIF4G is fundamental to the assembly of the cap-binding complex. The 3D structure of eIF4E from several organisms was formerly deciphered [3, 47–50]. Its N-terminal part is flexible and not conserved, while the C-terminus adopts a conserved structural signature. eIF4E acquires the shape of a baseball glove with a dedicated pocket for binding of the methylated guanine, which contains a sandwich of three Trp residues (W56, 102 and 166, according to the murine numbering). In addition, the basic residues Arg112, 157 and 162 interact with the negatively charged phosphate moieties of the cap structure. eIF4E binds eIF4G and several translation repressors, such as 4E-BP, through several conserved residues in its C-terminus. The high degree of structural conservation among orthologs of eIF4E enables homologs from different species to functionally replace each other in a yeast-based genetic complementation assay [51].The trypanosomatid cap-4 binding proteins have gone through structural adaptations during their evolution to adjust to binding of the highly modified cap-4 structure. Their genomes encode four isoforms of eIF4E [46, 52]. Although homology modeling of the four proteins supports a structural conservation of the cap-binding pocket, none of the four paralogs could complement the missing function of the yeast eIF4E, indicating high functional divergence from their higher eukaryotic orthologs [46]. Thus, deciphering the roles of the different isoforms is complex, especially since efficient cell-free systems for in vitro reconstitution of translation initiation using parasite mRNAs are not available for trypanosomatids. Biophysical assays using a chemically synthesized cap-4 and intermediate cap analogues [53], showed that the four eIF4E, denoted LeishIF4E-1 through LeishIF4E-4, vary in their cap-binding specificities. Trp fluorescence assays indicated that LeishIF4E-1 and LeishIF4E-4 bound m7GTP and cap-4 with comparable affinities, LeishIF4E-3 bound mainly to m7GTP and hardly to cap-4, whereas LeishIF4E-2 showed a great preference to cap-4, as compared to m7GTP [46]. In agreement, the endogenous LeishIF4E-1 and LeishIF4E-4 were eluted from m7GTP-Sepharose column [46, 54]. Despite the ability of LeishIF4E-3 to bind m7GTP in the fluorescence titration assays, the recombinant and endogenous proteins showed very weak binding to m7GTP-Sepharose column, for reasons not fully understood yet [46].The subunits of theLeishmaniaeIF4F complex were examined by pulldown analysis and by monitoring their migration profile on sucrose gradients. These analyses led to the conclusion that LeishIF4E-4 is the most probable candidate to serve as the conventional eIF4E in promastigotes, and is part of the parasite LeishIF4F cap-4 binding complex. LeishIF4E-4 was shown to interact with LeishIF4G-3, a protein that contains a “middle of eIF4G” (MIF4G) domain [54, 55], which is responsible for recruiting LeishIF4A-1. A parallel interaction was shown for the Trypanosoma brucei ortholog [54]. The three eIF4F subunits comigrate on sucrose density gradients and are found in fractions that are expected to contain the pre-initiation complex [55].Silencing experiments by RNAi were performed on the fourT. bruceiisoforms [54] in an attempt to reveal their function. Downregulation of TbIF4E-4 inhibited the growth of bloodstream form, but not procyclic cells. This result does not coincide with data obtained for the Leishmaniaparalog, since LeishIF4E-4 binds m7GTP and LeishIF4G-3 mainly in promastigotes, and fails to perform these activities upon exposure to mammalian-like temperatures and in axenic amastigotes [56]. Furthermore, the migration pattern of LeishIF4E-4 in sucrose gradients shows that in heat-shocked cells this protein no longer forms large complexes. Altogether, the activity of LeishIF4E-4 is dramatically reduced at prolonged elevated temperatures [55], through a mechanism which is yet to be resolved. The differences observed between the stage-specific function of the LeishIF4E-4 and TbIF4E-4 described above could originate from a variable experimental setup, or alternatively, from differences between the species. It is also possible that after the final differentiation to the mammalian amastigote life-form, LeishlF4E-4 resumes its activity.The dual silencing of TbIF4E-4 and TbIF4E-1 led to a growth arrest of procyclic parasites, suggesting that the two proteins may have a partially redundant function. Individual silencing of TbIF4E-4 or TbIF4E-1 was harmful only to the bloodstream life form. LeishIF4E-1, theLeishmania ortholog of TbIF4E-1, is the only eIF4E isoform that maintains its expression at elevated temperatures and in axenic amastigotes of Leishmania. The other three orthologs were downregulated under these conditions, excluding that they have a role in translation during heat shock [46, 56]. It is, therefore, possible that LeishIF4E-1 is associated with translation under stress and in amastigotes. TbIF4E-1, as well as its Leishmania ortholog LeishIF4E-1, do not interact efficiently with TbIF4G-3 or LeishIF4G-3, respectively [54, 56], suggesting that this isoform does not participate in building an eIF4F complex and therefore, could promote alternative pathways of translation, possibly in an eIF4G-independent manner. LeishIF4E-1 could either have an active role in translation which has not yet been resolved, or alternatively, it could passively protect the cap structure, if cap-independent mechanisms are practiced.Elimination of TbIF4E-2, had no effect onT. brucei growth in both life forms [54]. The Leishmana, LeishIF4E-2, ortholog comigrated with heavy polysomes in sucrose gradients [46], unlike typical translation initiation factors that are found in lighter fractions [57, 58]. The RNAi experiments in T. brucei indicated that among the four paralogs, only TbIF4E-3 was essential for growth of both procyclic and bloodstream life forms. Its silencing caused a reduction in incorporation of a radiolabeled amino acid into newly synthesized polypeptides, and it was, therefore, suggested that TbIF4E-3 serves as a translation initiation factor [54]. However, the reduced translation rates occurred only after 72 hours, while the silencing was observed already after 24 hours. It could therefore be a downstream effect of other processes that inhibit cell growth in the absence of TbIF4E-3. TbIF4E-3 was reported to be part of RNA granules in T. brucei[59, 60] and could, therefore, be associated with trafficking of mRNAs either to storage bodies or to actively translating polysomes. The low affinity of the Leishmaniaortholog LeishIF4E-3 to cap-4 contradicts its definition as a typical translation initiation factor. Thus, although TbIF4E-3 interacts with a MIF4G domain protein, TbIF4G-4, its precise role remains elusive.


## 4. eIF4E Binding Proteins of Trypanosomatids

Trypanosomatids encode several eIF4G candidates that contain the MIF4G domain. This element consists of several HEAT repeats [61], typical of all eIF4G proteins in higher eukaryotes [62]; it is responsible for binding to eIF4A as well as to RNA. The C-terminus of the human eIF4G contains a second site for interaction with eIF4A and its N-terminus carries binding sites for eIF4E and PABP. A consensus peptide motif, YXXXXLΦ, in eIF4G and in 4E-BP is responsible for binding to eIF4E. Substitution of the conserved Y or LΦ residues in the motif abrogates the eIF4E-eIF4G interaction [63].The identity of theLeishmania eIF4G ortholog was deduced by its co-purification and interaction with LeishIF4E-4. This binding was monitored in yeast two-hybrid assays and pulldown experiments [52, 54–56].LeishIF4G-3 and TbIF4G-3 contain a typical MIF4G domain [52, 55], but they vary from their mammalian counterpart in other parts of the protein. For example, the N-terminus of LeishIF4G-3 is short (50 amino acids) and contains the LeishIF4E-4 binding-peptide, but it cannot interact with PABP in a yeast two-hybrid assay. This peptide motif is only partially conserved with the YXXXXLΦ consensus, and binding to LeishIF4E-4 requires the presence of a Phe residue at position 4 [63], in addition to Tyr and Leu at positions 1 and 6 of the peptide. A partial requirement was observed for the Gly and Glu residues at positions 3 and 8 [55]. Altogether, this indicates a certain degree of variability from the eukaryote consensus sequence.Other regions of the parasite eIF4G are also a source for variability, as compared to its human counterpart. eIF4G is a scaffold protein that links the 5′ and 3′ ends by interacting with both eIF4E and PABP. However, unlike in higher eukaryotes, mRNA circularization occurs through an interaction between the N-terminus of LeishIF4E-4 and LeishPABP-1. Elimination of this LeishIF4E-4 domain prevents its binding to LeishPABP-1 [56]. Therefore, LeishIF4E-4 along with LeishIF4G-3 and LeishIF4A-1, comprise a typical eIF4F complex in promastigotes [64], see Figure 2. The binding between LeishIF4G-3 and LeishIF4E-4 is eliminated at elevated temperatures and in axenic amastigotes, supporting that under these conditions translation may proceed through alternative pathways, possibly involving LeishIF4E-1.Interacting partners of cap-binding proteins inLeishmania. The typical eIF4F complex of higher eukaryotes (a) and Leishmania promastigotes (b) are shown. A table summarizing the known interacting partners of the Leishmania eIF4F complex are shown in Table 1. Accession numbers of the described proteins are: LeishIF4E-1—LmjF27.1620, LeishIF4E-3—LmjF28.2500, LeishIF4E-4—LmjF30.0450, LeishIF4G-3—LmjF16.1600, LeishIF4G-4—LmjF36.6060, LeishIF4A-1—LmjF01.0780 and LmjF01.0770, LeishPABP-1—LmjF35.5040, Leish4E-IP—LmjF35.3980.
(a)(b)Table 1ProteinInteracts withSourceLeishIF4E-1Leish4E-IPZinoviev et al., 2011 [56]LeishIF4E-3LeishIF4G-4Freire et al., 2011 [54]LeishIF4E-4LeishIF4G-3Yoffe et al., 2009 [55]LeishIF4A-1LeishIF4G-3Zinoviev et al., 2011 [56]LeishPABP-1LeishIF4G-3Zinoviev et al., 2011 [56]An additional eIF4E-eIF4G pairing was reported for TbIF4E-3 and TbIF4G-4 [54]. The authors of this study propose that TbIF4E-3 is involved in translation initiation. This assumption is not supported by studies in Leishmania that excluded the copurification of these two proteins over a m7GTP affinity column [54]. Furthermore, LeishIF4E-3 failed to bind cap-4 in fluorescence titration assays, thus making it an unlikely candidate to serve as a conventional translation initiation factor [46].Another well-known eIF4E binding protein is 4E-BP of higher eukaryotes. It is a highly conserved protein that is expressed in most eukaryotes, except forC. elegans. The trypanosomatid genome database also does not contain any ortholog of the consensus 4E-BP (~10 kDa). However, affinity co-purification assays identified a novel LeishIF4E-interacting protein, denoted Leish4E-IP (~80 kDa) [56]. Leish4E-IP and the eukaryotic 4E-BP show no sequence homology, but share a predicted unstructured nature. Leish4E-IP binds mainly to LeishIF4E-1 with a tight requirement for the consensus YXXXXLΦ peptide [63]. Furthermore, although Leish4E-IP is expressed at all life stages, its binding to LeishIF4E-1 is observed only in promastigotes, suggesting that it participates in framing a stage-specific program of gene expression, via a mechanism that is yet to be resolved. Posttranslational modifications, such as phosphorylations, are likely to be involved. Data mining in the Leishmania genome revealed three orthologs of the TOR kinase. TOR 1 and 2 are essential, as null mutants could not be generated, whereas TOR3 could be eliminated. However, null mutants of TOR3 were nonvirulent and were impaired in their ability to survive within macrophages [65].


## 5. eIF4A Isoforms of Trypanosomatids

eIF4A is a member of the DEAD-box RNA helicases [66]. It promotes scanning of the 5′ UTR by the 43S PIC, via unwinding of the secondary RNA structures, until it arrives at the initiator AUG codon. DEAD-box proteins participate in a multitude of processes related to transcription, RNA processing, export and translation. The genome of most eukaryotes encodes for more than a single eIF4A isoform. In addition to the translation initiation factor eIF4A, another paralog was reported to be part of the exon junction complex (EJC) [67]. The genome of T. bruceiencodes two isoforms of eIF4A. TbIF4AI and its Leishmania homolog, LeishIF4A-1, are part of the eIF4F cap-binding complex. Tb4AI is cytoplasmic and, as expected, interacts with TbIF4G-3 [52, 56]. Its downregulation caused a dramatic decrease in protein synthesis. TbIF4AIII is predominantly nuclear. It is essential, but its silencing hardly affects protein synthesis, and the resulting lethal effects are delayed, as compared to TbIF4AI. Thus TbIF4AI is the translation initiation factor that comprises the cap-4 binding complex, while the role of TbIF4AIII is not yet understood, although it may assign a similar role as in higher eukaryotes [64].


## 6. Poly(A) Binding Proteins of Trypanosomatids

Yeast encode a single PABP, whereas metazoans express several paralogs, with a tissue, or embryonic-specific pattern of expression [68]. The typical PABP are closely related, and consist of four conserved RRM domains. Their C-terminus promotes protein-protein interactions with their binding partners [69].Elongated poly(A) tails are crucial for translation, but are also involved in a variety of processes related to RNA processing, export and stabilization. The mammalian PABP associates with eIF4G, resulting in transient circularization of the mRNA during translation initiation [70]. This interaction stabilizes the initiation complex and enhances translation. It is therefore targeted by endogenous regulators, such as the PABP-binding proteins PAIP1 and PAIP2, which can enhance or repress the activity of PABP, respectively [71]. PABP is also targeted by viral proteases, as part of their strategy to take over the cellular translational machinery [72].T. brucei and T. cruzi genomes contain two PABP paralogs [73, 74]. Both are essential, indicating that they have different cellular functions. The leishmanias encode another unique isoform, PABP3 [73]. PABP1 was associated with components of the cap-binding complex [56, 73], suggesting that it is the cap-dependent translation initiation factor. To date, it is the only isoform that was shown to bind directly to a translation initiation factor. However, unlike in higher eukaryotes, it interacts with eIF4E instead of eIF4G. The higher eukaryote PABP shuttles between the nucleus and the cytoplasm, but it is mostly cytoplasmic. All three Leishmania isoforms are indeed cytoplasmic, but inhibition of transcription causes PABP2 and PABP3 to accumulate in the nucleus. Their role is yet to be identified.


## 7. Translation under Stress Conditions in Trypanosomatids

The untranslated regions of trypanosomatid mRNAs, mostly those located downstream to the coding sequences, play a key role in differential expression of genes during the life cycle. This was established by the use of reporter systems for Hsp70 [75, 76] and Hsp83 [77, 78] of Leishmania. It was also established, that despite the key role of mRNA stability, elements that affected translation of Hsp83 alone could be identified. Large mutations that destabilized the Hsp83 mRNA at elevated temperatures did not interfere with its preferential translation, as long as the regulatory element for translation was included in the 3′ UTR [77]. Fine deletions finally identified a regulatory element of 30 nucleotides (positions 312–341), containing a stretch of polypyrimidines. This region was shown to be part of an RNA structure that was predicted with high probability [79], using the UNAfold algorithm [80]. A biophysical evaluation of the mRNA melting curves was performed to examine the role of secondary structures in the regulatory region. Incubation of the corresponding wild-type mRNA fragment (1–472) led to its melting at elevated temperatures (35°C). The mutant element, that did not induce preferential translation, failed to show a similar pattern. It was, therefore, assumed that preferential translation of LeishmaniaHSP83 during stress was promoted by melting of the regulatory region in the mRNA [79]. It is interesting to note that the element is not conserved throughout trypanosomatids, emphasizing the role of RNA structure in this mode of regulation.An additional RNA element that confers stage-specific translation was identified in the theLeishmania Amastin genes. It was first mapped as an element of 450 nucleotides within their 3′ UTRs. Additional experiments narrowed this region down to 150 nucleotides that enhance translation of the harboring transcript in the amastigote life stage. The Amastin element was found in other amastigote-specific genes of Leishmania, such as Hsp100 [81, 82]. However, it bears no conservation to that of Hsp83. Furthermore, no defined secondary structure was reported for the Amastin RNA regulatory sequence. Therefore, the preferential translation conferred by the two elements is functionally distinct.Trypanosomatids experience a broad range of environmental stresses during their complex life cycle. In addition to the switch between vector and host, they can also suffer from a shortage in sugars within the insect vector, due to its nutritional diet. Under these conditions, a transient arrest of translation is required until the stress is relieved. One common way to arrest global translation in higher eukaryotes is through inactivation of eIF2α, which can be achieved by phosphorylation of its conserved Ser51 residue. Indeed, phosphorylation of the T. brucei paralog occurs on the corresponding Thr169 [83] and on Thr 166 in the Leishmania eIF2α [84].  Since the trypanosomatid ortholog has a long non-conserved N-terminal extension, it shifts the position of the phosphorylated Thr. This phosphorylation of the Leishmania eIF2α is associated with stage transformation [84]. However, a mutant of T. brucei strain that encodes for a mutated eIF2α (T169A/−) and therefore cannot undergo phosphorylation, showed no effect on translation or on the ability to form heat-shock stress granules [59]. Thus, phosphorylation of Thr169 alone is most probably not sufficient to inhibit translation. Three potential eIF2α kinases (TbeIF2K1 to -3) were identified in the genome of T. brucei [83]. TbeIF2K2 was extensively studied, and shown to localize in the membrane of the flagellar pocket, a site that is known to promote exo- and endocytosis. This kinase phosphorylates the trypanosomatid eIF2α, as well as its mammalian counterpart. However, TbeIF2α is not a substrate for the mammalian GCN2 or PKR.A second conserved mechanism for global translational arrest in higher eukaryotes involves the dephosphorylation of 4E-BP, although recent reports show numerous exceptions [26], indicating that this pathway is much more complex [23]. In most cases, the dephosphorylated 4E-BP binds to eIF4E and prevents assembly of the cap-dependent translation initiation complex. Trypanosomatid genomes lack the consensus 4E-BP and this pathway to achieve translational arrest is not functional as well. Thus, the mechanisms that confer a global decrease in translation in trypanosomatids remain unclear.Differentiation of trypanosomatids from the insect-specific to the mammalian life-form is induced by extreme environmental switches. This raises the question of how these parasites deal with the damaging effects of the prolonged stresses, which would be deadly to other eukaryotes. In the absence of conventional mechanisms for transcriptional activation of protein coding genes [36], their differential pattern of expression along the life cycle is induced by post-transcriptional regulatory mechanisms [38], including splicing and mRNA stability, as well as translation [76, 77, 85]. Transcriptome [86, 87] and proteome [88] screens performed with Leishmaniaparasites during their axenic differentiation were published and are publicly available. In total, they reveal that differential gene expression occurs due to changes in mRNA levels at the early stages of differentiation. However, at later stages, translation and posttranslational regulation mechanisms are more influential [84]. It was also noted by the Zilberstein group that during the initial period at which signaling for differentiation takes place, translation decreased dramatically. Expression of ribosomal proteins was downregulated, eIF2α was phosphorylated and a general decrease in the amount of polysomes was observed. These effects were mainly transient, and translation resumed upon completion of the differentiation process [88].


## 8. What Is Next?

The research of trypanosomatids is rapidly advancing, and new tools are constantly being developed. The use of RNAi, which currently is restricted toT. brucei, is about to be developed for Leishmania braziliensis [88], the only Leishmania species that encodes all the components of this pathway. It should be interesting to see if silencing of the various factors described in this review confers similar effects in Leishmania as compared to Trypanosoma.Another interesting point refers to the elegant strategy developed byLeishmania parasites to overtake their host cells. It was recently shown that the GP63 protease, which is secreted by the parasites into the infected macrophage, causes an efficient shutdown of the host protein synthesis. This is achieved by cleavage of mTOR, causing the constitutive dephosphorylation and activation of 4E-BP [89]. However, other targets of mTOR could presumably be affected, resulting in inhibitory effects on the macrophage metabolism.Several recent publications describe the translational machinery of trypanosomatids and highlight its evolutionary variability, as compared to higher eukaryotes. The reported diversifications provide an exciting target for novel therapeutic approaches [90]. Potential drug targets that could be pursued include protein-RNA and protein-protein interactions that promote assembly of the translation initiation complex. For example, interactions between the cap-binding proteins and the unique cap-4 structure are of interest, as well as the binding between LeishIF4E-4 and LeishIF4G-3. TbIF4E-3, the ortholog of LeishIF4E-3, is also an interesting drug target, as it was shown to be essential in the blood stream form of T. brucei.



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*Source: 813718-2012-07-08.xml*