# Fertilization and Colors of Plastic Mulch Affect Biomass and Essential Oil of Sweet-Scented Geranium

Authors: Anderson de Carvalho Silva; Arie Fitzgerald Blank; Wallace Melo dos Santos; Paloma Santana Prata; Péricles Barreto Alves; Maria de Fátima Arrigoni-Blank
Journal: The Scientific World Journal
 (2014)

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## Abstract

Sweet-scented geranium (Pelargonium graveolens L’Hér), a plant belonging to the Geraniaceae family, has medicinal and aromatic properties and is widely used in the cosmetic, soap, perfume, aromatherapy, and food industries. The aim of this study was to evaluate the influence of fertilization and the use of different colors of plastic mulch on sweet-scented geranium biomass and essential oil. Three colors of plastic mulch (black, white, and silver-colored) and a control without plastic mulch were assessed along with three fertilizers (20,000 L·ha−1 of cattle manure; 1,000 kg·ha−1 of NPK 3-12-6; and 20,000 L·ha−1 of cattle manure + 1,000 kg·ha−1 of NPK 3-12-6 fertilizer) and a control without fertilizer. The absence of a soil cover negatively influenced the agronomical variables, while coverage with plastic mulch was associated with increased biomass. The use of fertilizer had no effect on the evaluated agronomic variables. When cattle manure and NPK 3-12-6 were used together, combined with white or black plastic mulch, the highest yields of essential oil were obtained. For the silver-colored plastic mulch, higher amounts of essential oil (6,9-guaiadien) were obtained with mineral fertilizer.


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## Body

## 1. Introduction

ThePelargonium (Geraniaceae) genus contains around 280 herbaceous, shrub, and subshrub species, including annuals and perennials that typically originated from southern Africa [1].Pelargonium graveolensL’Hér is one of the most important species in this genus and is an aromatic shrub species that can reach up to 1.3 m in height. This geranium is native to Cape Province, South Africa, and is grown in many regions worldwide, including the Reunion Islands, Algeria, southern France, Spain, Morocco, Madagascar, Congo, and Russia, for the production of its essential oil [2–6]. The geranium essential oil is an important material for the cosmetic, soap, perfume, aromatherapy, and food industries [2, 7–10]. It is also used in popular medicines against dysentery, diarrhea, colds, and lung infections [3, 11].The production of essential oils in plants may be altered by environmental or ontogenic factors, such as the physical-chemical characteristics of the soil, moisture, temperature, developmental stage, and phenology [12]. These factors have therefore been evaluated to determine how they influence the production of volatile compounds in medicinal and aromatic species [8, 13–15].Soil composition is one of the determining factors for crop establishment and may be altered to meet the nutritional needs of plants. Agricultural inputs, namely, chemical or organic fertilizers, can be used for this purpose. Organic fertilizers naturally reset nutritional characteristics and rely on the production of organic matter by decomposition, which is an important source of nutrients for plants, microflora, and terrestrial fauna. In the soil, organic matter provides nutrients and has an important role as a conditioner of the physical, chemical, and biological properties of the soil [16]. The traditional cultivation of geranium combines chemical fertilizer and organic matter inputs. With respect to essential oil production, the cultivation ofP. graveolensis more profitable when the crop is grown in soils rich in organic matter [17].For the use of proper growth conditions and increase of the production of active constituents it is necessary to develop agricultural technologies for a correct cultivation of aromatic plants [16, 18]. Mulching is a common technology used in the cultivation of medicinal and aromatic species to increase the production of active ingredients [19]. Among other benefits, the use of mulch regulates surface radiation, promotes vegetative growth and productivity, increases insect repellence, controls weeds, decreases water loss by evaporation, and facilitates harvesting [20].The aim of this study was to evaluate the effect of organic-mineral fertilizers and soil coverage with plastic mulch on sweet-scented geranium (P. graveolens) biomass and essential oil.


## 2. Materials and Methods

### 2.1. Plant Materials

Plants of sweet-scented geranium (Pelargonium graveolens L’Hér) genotype UFS-PEL001 were propagated by cuttings. The plants were maintained in 1500 cm3 plastic bags containing a 1 : 1 mixture of soil and sand as substrate for 30 days and then transplanted to the field. Genotype exsiccates were deposited in the ASE Herbarium of the Federal University of Sergipe (UFS), under the record number ASE-14844.


### 2.2. Experimental Procedure

The assay was conducted at the “Campus Rural da UFS” Research Farm, located in São Cristóvão, Sergipe State, Brazil, from 2008 to 2009. The climate of the region is tropical semiarid, and the soil is classified as Red-Yellow Argisol with low fertility. The chemical characteristics of the soil are as follows:pH=5.4 in water; P=2.3 mg·dm−3; K (Mehlich 1) = 0.09 cmolc·dm−3; Ca + Mg [21] = 2.70 cmolc·dm−3; and organic matter = 21.1 g·dm−3.A randomized block design was used for the experiment, with three replicates in a split-plot design. In the plots, three different colored plastic mulches (black, white, and silver-colored) were tested in addition to the control with no plastic mulch. In the subplots, three fertilizer treatments (20,000 L·ha−1 of cattle manure; 1,000 kg·ha−1 of NPK 3-12-6; and 20,000 L·ha−1 of cattle manure + 1,000 kg·ha−1 of NPK 3-12-6) were tested in addition to the control without fertilizer. Fertilizer was applied 15 days before transplantation of the plants to the field.The spacing between rows and between plants was 50 cm. The beds were 90 cm wide, and each plot contained four plants cultivated in two rows. A drip irrigation system was used. Planting in the field was performed on May 28, and the first harvest was realized on August 26. Subsequent harvests were realized 90 days apart.At each harvest, the height and canopy diameter of each plant was measured before cutting the plants and after cutting the fresh weight was measured. To determine the moisture content, three 100 g fresh leaf samples were dried in an oven dryer at 105°C until constant weight.


### 2.3. Essential Oils

The essential oils of the fresh leaves were obtained by steam distillation using a Clevenger apparatus [22] for 2 h and 40 min [23]. The content percentage was estimated using the dry matter weight (v/m) of the three 100 g leaf samples.


### 2.4. Analysis of Essential Oils

The analysis of the essential oil chemical composition was performed in a gas chromatograph coupled to a mass spectrometer (GC-MS) (Shimadzu, model QP 5050A) equipped with an AOC-20i autoinjector (Shimadzu) and a fused-silica capillary column (5%-phenyl-95%-dimethylpolysiloxane, 30 m × 0.25 mm id., 0.25μm film, J&W Scientific). Helium was used as the carrier gas at a flow rate of 1.2 mL/min. The temperature program was as follows: 50°C for 1.5 min, temperature increase at 4°C/min until reaching 200°C, temperature increase at 15°C/min until reaching 250°C, and 250°C for 5 min. The injector temperature was 250°C, and the detector (or interface) temperature was 280°C. The injection volume of ethyl acetate was 0.5 μL, the partition rate of the injected volume was 1 : 87, and the column pressure was 64.20 kPa. The mass spectrometer conditions were as follows: ionic capture detector impact energy of 70 eV and scanning speed 0.85 scan/s from 40 to 550 Da.Quantitative analysis of the chemical constituents was performed by flame ionization gas chromatography (FID), using a Shimadzu GC-17A (Shimadzu Corporation, Kyoto, Japan) instrument, under the following operational conditions: capillary ZB-5MS column (5% phenyl-arylene-95%-dimethylpolysiloxane) and fused-silica capillary column (30 m × 0.25 mm i.d. × 0.25μm film thickness) from Phenomenex (Torrance, CA, USA), under same conditions as reported for the GC-MS. Quantification of each constituent was estimated by area normalization (%). Compound concentrations were calculated from the GC peak areas and they were arranged in order of GC elution.The essential oil components were identified by comparing their mass spectra with the available spectra in the equipment database (NIST05 and WILEY8). Additionally, the measured retention indices were compared with those in the literature [24]. The relative retention indices (RRI) were determined using the Vandendool and Kratz [25] equation and a homologous series of n-alkanes (C8-C18) injected under the chromatography conditions described above.


### 2.5. Statistical Analysis

We analyzed the following variables during the 12-month period: survival (%), plant height (cm), canopy diameter (cm), leaf fresh weight (g·plant−1), leaf moisture (%), essential oil content (%), and yield (mL·plant−1). The content (%) of the following chemical constituents was determined: citronellol, geraniol, citronellyl formate, linalool, 6,9-guaiadien, geranyl formate, geranial, and isomenthone.The means of the variables were subjected to the analysis of varianceF test and were compared using the Scott-Knott test at 5% probability.


## 2.1. Plant Materials

Plants of sweet-scented geranium (Pelargonium graveolens L’Hér) genotype UFS-PEL001 were propagated by cuttings. The plants were maintained in 1500 cm3 plastic bags containing a 1 : 1 mixture of soil and sand as substrate for 30 days and then transplanted to the field. Genotype exsiccates were deposited in the ASE Herbarium of the Federal University of Sergipe (UFS), under the record number ASE-14844.


## 2.2. Experimental Procedure

The assay was conducted at the “Campus Rural da UFS” Research Farm, located in São Cristóvão, Sergipe State, Brazil, from 2008 to 2009. The climate of the region is tropical semiarid, and the soil is classified as Red-Yellow Argisol with low fertility. The chemical characteristics of the soil are as follows:pH=5.4 in water; P=2.3 mg·dm−3; K (Mehlich 1) = 0.09 cmolc·dm−3; Ca + Mg [21] = 2.70 cmolc·dm−3; and organic matter = 21.1 g·dm−3.A randomized block design was used for the experiment, with three replicates in a split-plot design. In the plots, three different colored plastic mulches (black, white, and silver-colored) were tested in addition to the control with no plastic mulch. In the subplots, three fertilizer treatments (20,000 L·ha−1 of cattle manure; 1,000 kg·ha−1 of NPK 3-12-6; and 20,000 L·ha−1 of cattle manure + 1,000 kg·ha−1 of NPK 3-12-6) were tested in addition to the control without fertilizer. Fertilizer was applied 15 days before transplantation of the plants to the field.The spacing between rows and between plants was 50 cm. The beds were 90 cm wide, and each plot contained four plants cultivated in two rows. A drip irrigation system was used. Planting in the field was performed on May 28, and the first harvest was realized on August 26. Subsequent harvests were realized 90 days apart.At each harvest, the height and canopy diameter of each plant was measured before cutting the plants and after cutting the fresh weight was measured. To determine the moisture content, three 100 g fresh leaf samples were dried in an oven dryer at 105°C until constant weight.


## 2.3. Essential Oils

The essential oils of the fresh leaves were obtained by steam distillation using a Clevenger apparatus [22] for 2 h and 40 min [23]. The content percentage was estimated using the dry matter weight (v/m) of the three 100 g leaf samples.


## 2.4. Analysis of Essential Oils

The analysis of the essential oil chemical composition was performed in a gas chromatograph coupled to a mass spectrometer (GC-MS) (Shimadzu, model QP 5050A) equipped with an AOC-20i autoinjector (Shimadzu) and a fused-silica capillary column (5%-phenyl-95%-dimethylpolysiloxane, 30 m × 0.25 mm id., 0.25μm film, J&W Scientific). Helium was used as the carrier gas at a flow rate of 1.2 mL/min. The temperature program was as follows: 50°C for 1.5 min, temperature increase at 4°C/min until reaching 200°C, temperature increase at 15°C/min until reaching 250°C, and 250°C for 5 min. The injector temperature was 250°C, and the detector (or interface) temperature was 280°C. The injection volume of ethyl acetate was 0.5 μL, the partition rate of the injected volume was 1 : 87, and the column pressure was 64.20 kPa. The mass spectrometer conditions were as follows: ionic capture detector impact energy of 70 eV and scanning speed 0.85 scan/s from 40 to 550 Da.Quantitative analysis of the chemical constituents was performed by flame ionization gas chromatography (FID), using a Shimadzu GC-17A (Shimadzu Corporation, Kyoto, Japan) instrument, under the following operational conditions: capillary ZB-5MS column (5% phenyl-arylene-95%-dimethylpolysiloxane) and fused-silica capillary column (30 m × 0.25 mm i.d. × 0.25μm film thickness) from Phenomenex (Torrance, CA, USA), under same conditions as reported for the GC-MS. Quantification of each constituent was estimated by area normalization (%). Compound concentrations were calculated from the GC peak areas and they were arranged in order of GC elution.The essential oil components were identified by comparing their mass spectra with the available spectra in the equipment database (NIST05 and WILEY8). Additionally, the measured retention indices were compared with those in the literature [24]. The relative retention indices (RRI) were determined using the Vandendool and Kratz [25] equation and a homologous series of n-alkanes (C8-C18) injected under the chromatography conditions described above.


## 2.5. Statistical Analysis

We analyzed the following variables during the 12-month period: survival (%), plant height (cm), canopy diameter (cm), leaf fresh weight (g·plant−1), leaf moisture (%), essential oil content (%), and yield (mL·plant−1). The content (%) of the following chemical constituents was determined: citronellol, geraniol, citronellyl formate, linalool, 6,9-guaiadien, geranyl formate, geranial, and isomenthone.The means of the variables were subjected to the analysis of varianceF test and were compared using the Scott-Knott test at 5% probability.


## 3. Results

### 3.1. Agronomical Variables

The use of plastic mulch is common in vegetable production, but the effect of this mulching approach has not been widely studied in medicinal and aromatic plants.The results from the present study indicate that the interaction between the type of plastic mulch and the type of fertilizer was significant only for the yield of essential oil. The use of cattle manure alone or cattle manure + NPK 3-12-6, combined with white plastic mulch, produced a higher essential oil yield than did the same treatment with black or silver-colored plastic mulch (Table1). As for the other variables, the lack of plastic mulch significantly reduced survival and leaf fresh weight and the essential oil yield of the plants from the first two harvests. For the third and fourth harvests, reductions were observed for all the variables (Table 2).Table 1
The effect of different colors of plastic mulch and fertilizers on content and yield of sweet-scented geranium essential oil.
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver-colored
Essential oil content (%)
Control
1.30aA
1.42aA
0.87aA
1.16aA
Cattle manure
1.32aA
1.51aA
1.15aA
0.96aA
NPK 3-12-6
1.23aA
1.14aA
1.16aA
1.26aA
Cattle manure + NPK 3-12-6
1.30aA
1.25aA
1.08aA
1.16aA
Essential oil yield (mL·plant−1)
Control
0.32aA
0.49aA
0.26aA
0.52aA
Cattle manure
0.21aB
1.04aA
0.42aB
0.59aB
NPK 3-12-6
0.21aA
0.71aA
0.45aA
0.62aA
Cattle manure + NPK 3-12-6
0.18aB
1.02aA
0.64aB
0.97aA
Lowercase letters indicate differences within columns, and uppercase letters indicate differences within rows. Values followed by the same letter are not statistically different based on the Scott-Knott test (P≤0.05).Table 2
The effect of different colors of plastic mulch and fertilizers on the survival and leaf fresh weight of sweet-scented geranium.
Survival (%)
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver colored
First harvest
Control
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure
58.33Aa
91,66Aa
100.00Aa
100.00Aa
NPK 3-12-6
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
25.00Bb
100.00Aa
100.00Aa
100.00Aa
Second harvest
Control
58.33Aa
91.66Aa
100.00Aa
100.00Aa
Cattle manure
58.33Aa
100.00Aa
100.00Aa
100.00Aa
NPK 3-12-6
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
16.66Ba
100.00Aa
100.00Aa
100.00Aa
Third harvest
Control
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure
58.33Aa
91.66Aa
100.00Aa
100.00Aa
NPK 3-12-6
41.66Ba
100.00Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
16.66Bb
91.66Aa
100.00Aa
100.00Aa
Fourth harvest
Control
25.00Bb
91.66Aa
100.00Aa
100.00Aa
Cattle manure
33.33Ba
83.33Aa
100.00Aa
91.66Aa
NPK 3-12-6
33.33Ba
83.33Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
25.00Bb
91.66Aa
50.00Bb
100.00Aa
Leaf fresh weight (g·plant−1)
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver colored
First harvest
Control
107.65aA
164.33bA
150.07aA
303.09aA
Cattle manure
70.83Ba
346.65Aa
179.76Ba
319.15Aa
NPK 3-12-6
78.51Ba
303.38Aa
148.52Ba
248.25Aa
Cattle manure + NPK 3-12-6
62.53Ba
374.98Aa
261.36Aa
363.86Aa
Second harvest
Control
106.65Aa
311.32Aa
196.28Aa
372.15Aa
Cattle manure
82.21Aa
332.41Aa
232.34Aa
404.18Aa
NPK 3-12-6
65.17Ba
368.68Aa
215.04Ba
359.23Aa
Cattle manure + NPK 3-12-6
23.28Ba
299.97Aa
272.50Aa
305.56Aa
Third harvest
Control
141.66Ba
425.00Aa
187.50Ba
389.83Aa
Cattle manure
137.50Ba
555.00Aa
316.66Ba
482.44Aa
NPK 3-12-6
136.11Aa
434.16Aa
326.66Aa
334.71Aa
Cattle manure + NPK 3-12-6
21.66Ba
456.39Aa
324.16Aa
451.29Aa
Fourth harvest
Control
23.33Aa
23.33Aa
141.66Aa
269.16Aa
Cattle manure
55.00Aa
55.00Aa
183.99Aa
207.50Aa
NPK 3-12-6
63.33Aa
63.33Aa
203.33Aa
138.33Aa
Cattle manure + NPK 3-12-6
150.00Aa
150.00Aa
216.66Aa
289.16Aa
Lowercase letters indicate differences within columns, and uppercase letters indicate differences within rows. Values followed by the same letter are not statistically different based on the Scott-Knott test (P≤0.05).For the different fertilization treatments, there were no significant differences observed for the assessed variables (Table1).


### 3.2. Essential Oils

The contents of several of the analyzed compounds varied significantly when different types of fertilizers were used. In addition, there were variations in compound content when the plants were grown under different colored plastic mulches (Table3).Table 3
The effects of different colors of plastic mulch and fertilizers on contents of citronellol, geraniol, citronellyl formate, linalool, 6,9-guaiadien, geranyl formate, geranial, and isomenthone in sweet-scented geranium essential oil.
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver-colored
Citronellol (%) (RRI = 1223)
Control
24.68bB
28.18aA
26.95aB
30.42aA
Cattle manure
29.44aA
29.46aA
31.22aA
28.72aA
NPK 3-12-6
25.14bB
30.56aA
28.73aA
29.00aA
Cattle manure + NPK 3-12-6
23.68bB
29.44aA
29.61aA
30.64aA
Geraniol (%) (RRI = 1249)
Control
20.85aA
17.99aA
19.88aA
19.04aA
Cattle manure
18.99aA
19.39aA
20.63aA
20.28aA
NPK 3-12-6
21.86aA
17.80aB
18.21aB
16.66aB
Cattle manure + NPK 3-12-6
24.72aA
18.99aB
18.99aB
18.49aB
Citronellyl formate (%) (RRI = 1271)
Control
11.16aA
10.97aA
11.40aA
12.00bA
Cattle manure
11.69aA
11.67aA
12.15aA
10.69bA
NPK 3-12-6
11.11aA
11.87aA
12.16aA
14.28aA
Cattle manure + NPK 3-12-6
9.54aA
11.69aA
12.52aA
14.61aA
Linalool (%) (RRI = 1095)
Control
12.36aA
11.80aA
10.00aA
10.89aA
Cattle manure
10.31aA
11.42aA
10.06aA
8.99aA
NPK 3-12-6
9.95aA
13.08aA
13.55aA
12.82aA
Cattle manure + NPK 3-12-6
11.63aA
10.31aA
12.66aA
9.90aA
6,9-guaiadien (%) (RRI = 1442)
Control
6.19aA
5.95aA
5.74aA
4.39bB
Cattle manure
6.02aA
4.93aA
4.84aA
4.56bA
NPK 3-12-6
5.93aA
5.46aA
5.68aA
6.10aA
Cattle manure + NPK 3-12-6
5.07aA
6.02aA
5.04aA
5.87aA
Geranyl formate (%) (RRI = 1298)
Control
3.50aA
3.48aA
4.10aA
4.12aA
Cattle manure
3.63aA
3.73aA
3.55aA
3.79aA
NPK 3-12-6
3.25aA
3.12aA
3.91aA
4.62aA
Cattle manure + NPK 3-12-6
3.39aA
3.63aA
3.29aA
5.64aA
Geranial (%) (RRI = 1264)
Control
1.12aA
1.29aA
1.33aA
1.12aA
Cattle manure
1.18aA
0.79aA
0.00bB
1.26aA
NPK 3-12-6
1.18aA
1.13aA
1.20aA
1.08aA
Cattle manure + NPK 3-12-6
1.12aA
1.19aA
1.16aA
0.30bB
Isomenthone (%) (RRI = 1158)
Control
1.23aA
1.40aA
1.24aA
1.37aA
Cattle manure
1.18aA
1.18aA
0.93bA
1.24aA
NPK 3-12-6
0.99aB
1.40aA
1.31aA
1.31aA
Cattle manure + NPK 3-12-6
1.18aA
1.18aA
1.25aA
1.32aA
RRI: relative retention index.
Lowercase letters indicate differences within columns, and uppercase letters indicate differences within rows. Values followed by the same letter are not statistically different based on the Scott-Knott test (P≤0.05).There was an interaction between the various factors of the contents of citronellol, geraniol, 6,9-guaiadien, geranial, and isomenthone. The citronellyl formate content was only affected by colors of plastic mulch. The linalool and geranyl formate contents were not affected by types of fertilizers and colors of plastic mulches (Table3). Significant differences in citronellol content were observed for the different fertilizer treatments when no plastic mulch was used. In this case, the use of cattle manure as fertilizer led to an increased content of citronellol. There were significant differences in geraniol content for the different colors of plastic mulch with the NPK (3-12-6) and cattle manure + NPK (3-12-6) fertilizer treatments; greater geraniol content was observed when no plastic mulch was used compared to the other plastic mulch treatments. The citronellol content was greater when cattle manure was used as fertilizer, while geraniol production increased when NPK (3-12-6) was used.The lowest contents of 6,9-guaiadien were observed with the silver-colored plastic mulch using no fertilizer or cattle manure for fertilizing (Table3). The production of geranial was strongly reduced by two particular combinations: the use of a black plastic mulch with cattle manure and the use of a silver-colored plastic mulch with cattle manure + NPK 3-12-6 (Table 3). These observations highlight the negative influence of cattle manure combined with the plastic mulches on the geranial compound.Significant differences were also observed for citronellyl formate content, which was reduced when the silver-colored plastic mulch was combined with cattle manure or without fertilizer. The isomenthone content was lowest when NPK 3-12-6 was combined with no plastic mulch and when cattle manure was combined with the black plastic mulch.


## 3.1. Agronomical Variables

The use of plastic mulch is common in vegetable production, but the effect of this mulching approach has not been widely studied in medicinal and aromatic plants.The results from the present study indicate that the interaction between the type of plastic mulch and the type of fertilizer was significant only for the yield of essential oil. The use of cattle manure alone or cattle manure + NPK 3-12-6, combined with white plastic mulch, produced a higher essential oil yield than did the same treatment with black or silver-colored plastic mulch (Table1). As for the other variables, the lack of plastic mulch significantly reduced survival and leaf fresh weight and the essential oil yield of the plants from the first two harvests. For the third and fourth harvests, reductions were observed for all the variables (Table 2).Table 1
The effect of different colors of plastic mulch and fertilizers on content and yield of sweet-scented geranium essential oil.
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver-colored
Essential oil content (%)
Control
1.30aA
1.42aA
0.87aA
1.16aA
Cattle manure
1.32aA
1.51aA
1.15aA
0.96aA
NPK 3-12-6
1.23aA
1.14aA
1.16aA
1.26aA
Cattle manure + NPK 3-12-6
1.30aA
1.25aA
1.08aA
1.16aA
Essential oil yield (mL·plant−1)
Control
0.32aA
0.49aA
0.26aA
0.52aA
Cattle manure
0.21aB
1.04aA
0.42aB
0.59aB
NPK 3-12-6
0.21aA
0.71aA
0.45aA
0.62aA
Cattle manure + NPK 3-12-6
0.18aB
1.02aA
0.64aB
0.97aA
Lowercase letters indicate differences within columns, and uppercase letters indicate differences within rows. Values followed by the same letter are not statistically different based on the Scott-Knott test (P≤0.05).Table 2
The effect of different colors of plastic mulch and fertilizers on the survival and leaf fresh weight of sweet-scented geranium.
Survival (%)
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver colored
First harvest
Control
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure
58.33Aa
91,66Aa
100.00Aa
100.00Aa
NPK 3-12-6
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
25.00Bb
100.00Aa
100.00Aa
100.00Aa
Second harvest
Control
58.33Aa
91.66Aa
100.00Aa
100.00Aa
Cattle manure
58.33Aa
100.00Aa
100.00Aa
100.00Aa
NPK 3-12-6
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
16.66Ba
100.00Aa
100.00Aa
100.00Aa
Third harvest
Control
58.33Aa
100.00Aa
100.00Aa
100.00Aa
Cattle manure
58.33Aa
91.66Aa
100.00Aa
100.00Aa
NPK 3-12-6
41.66Ba
100.00Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
16.66Bb
91.66Aa
100.00Aa
100.00Aa
Fourth harvest
Control
25.00Bb
91.66Aa
100.00Aa
100.00Aa
Cattle manure
33.33Ba
83.33Aa
100.00Aa
91.66Aa
NPK 3-12-6
33.33Ba
83.33Aa
100.00Aa
100.00Aa
Cattle manure + NPK 3-12-6
25.00Bb
91.66Aa
50.00Bb
100.00Aa
Leaf fresh weight (g·plant−1)
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver colored
First harvest
Control
107.65aA
164.33bA
150.07aA
303.09aA
Cattle manure
70.83Ba
346.65Aa
179.76Ba
319.15Aa
NPK 3-12-6
78.51Ba
303.38Aa
148.52Ba
248.25Aa
Cattle manure + NPK 3-12-6
62.53Ba
374.98Aa
261.36Aa
363.86Aa
Second harvest
Control
106.65Aa
311.32Aa
196.28Aa
372.15Aa
Cattle manure
82.21Aa
332.41Aa
232.34Aa
404.18Aa
NPK 3-12-6
65.17Ba
368.68Aa
215.04Ba
359.23Aa
Cattle manure + NPK 3-12-6
23.28Ba
299.97Aa
272.50Aa
305.56Aa
Third harvest
Control
141.66Ba
425.00Aa
187.50Ba
389.83Aa
Cattle manure
137.50Ba
555.00Aa
316.66Ba
482.44Aa
NPK 3-12-6
136.11Aa
434.16Aa
326.66Aa
334.71Aa
Cattle manure + NPK 3-12-6
21.66Ba
456.39Aa
324.16Aa
451.29Aa
Fourth harvest
Control
23.33Aa
23.33Aa
141.66Aa
269.16Aa
Cattle manure
55.00Aa
55.00Aa
183.99Aa
207.50Aa
NPK 3-12-6
63.33Aa
63.33Aa
203.33Aa
138.33Aa
Cattle manure + NPK 3-12-6
150.00Aa
150.00Aa
216.66Aa
289.16Aa
Lowercase letters indicate differences within columns, and uppercase letters indicate differences within rows. Values followed by the same letter are not statistically different based on the Scott-Knott test (P≤0.05).For the different fertilization treatments, there were no significant differences observed for the assessed variables (Table1).


## 3.2. Essential Oils

The contents of several of the analyzed compounds varied significantly when different types of fertilizers were used. In addition, there were variations in compound content when the plants were grown under different colored plastic mulches (Table3).Table 3
The effects of different colors of plastic mulch and fertilizers on contents of citronellol, geraniol, citronellyl formate, linalool, 6,9-guaiadien, geranyl formate, geranial, and isomenthone in sweet-scented geranium essential oil.
Fertilizer
Colors of plastic mulch
Control
White
Black
Silver-colored
Citronellol (%) (RRI = 1223)
Control
24.68bB
28.18aA
26.95aB
30.42aA
Cattle manure
29.44aA
29.46aA
31.22aA
28.72aA
NPK 3-12-6
25.14bB
30.56aA
28.73aA
29.00aA
Cattle manure + NPK 3-12-6
23.68bB
29.44aA
29.61aA
30.64aA
Geraniol (%) (RRI = 1249)
Control
20.85aA
17.99aA
19.88aA
19.04aA
Cattle manure
18.99aA
19.39aA
20.63aA
20.28aA
NPK 3-12-6
21.86aA
17.80aB
18.21aB
16.66aB
Cattle manure + NPK 3-12-6
24.72aA
18.99aB
18.99aB
18.49aB
Citronellyl formate (%) (RRI = 1271)
Control
11.16aA
10.97aA
11.40aA
12.00bA
Cattle manure
11.69aA
11.67aA
12.15aA
10.69bA
NPK 3-12-6
11.11aA
11.87aA
12.16aA
14.28aA
Cattle manure + NPK 3-12-6
9.54aA
11.69aA
12.52aA
14.61aA
Linalool (%) (RRI = 1095)
Control
12.36aA
11.80aA
10.00aA
10.89aA
Cattle manure
10.31aA
11.42aA
10.06aA
8.99aA
NPK 3-12-6
9.95aA
13.08aA
13.55aA
12.82aA
Cattle manure + NPK 3-12-6
11.63aA
10.31aA
12.66aA
9.90aA
6,9-guaiadien (%) (RRI = 1442)
Control
6.19aA
5.95aA
5.74aA
4.39bB
Cattle manure
6.02aA
4.93aA
4.84aA
4.56bA
NPK 3-12-6
5.93aA
5.46aA
5.68aA
6.10aA
Cattle manure + NPK 3-12-6
5.07aA
6.02aA
5.04aA
5.87aA
Geranyl formate (%) (RRI = 1298)
Control
3.50aA
3.48aA
4.10aA
4.12aA
Cattle manure
3.63aA
3.73aA
3.55aA
3.79aA
NPK 3-12-6
3.25aA
3.12aA
3.91aA
4.62aA
Cattle manure + NPK 3-12-6
3.39aA
3.63aA
3.29aA
5.64aA
Geranial (%) (RRI = 1264)
Control
1.12aA
1.29aA
1.33aA
1.12aA
Cattle manure
1.18aA
0.79aA
0.00bB
1.26aA
NPK 3-12-6
1.18aA
1.13aA
1.20aA
1.08aA
Cattle manure + NPK 3-12-6
1.12aA
1.19aA
1.16aA
0.30bB
Isomenthone (%) (RRI = 1158)
Control
1.23aA
1.40aA
1.24aA
1.37aA
Cattle manure
1.18aA
1.18aA
0.93bA
1.24aA
NPK 3-12-6
0.99aB
1.40aA
1.31aA
1.31aA
Cattle manure + NPK 3-12-6
1.18aA
1.18aA
1.25aA
1.32aA
RRI: relative retention index.
Lowercase letters indicate differences within columns, and uppercase letters indicate differences within rows. Values followed by the same letter are not statistically different based on the Scott-Knott test (P≤0.05).There was an interaction between the various factors of the contents of citronellol, geraniol, 6,9-guaiadien, geranial, and isomenthone. The citronellyl formate content was only affected by colors of plastic mulch. The linalool and geranyl formate contents were not affected by types of fertilizers and colors of plastic mulches (Table3). Significant differences in citronellol content were observed for the different fertilizer treatments when no plastic mulch was used. In this case, the use of cattle manure as fertilizer led to an increased content of citronellol. There were significant differences in geraniol content for the different colors of plastic mulch with the NPK (3-12-6) and cattle manure + NPK (3-12-6) fertilizer treatments; greater geraniol content was observed when no plastic mulch was used compared to the other plastic mulch treatments. The citronellol content was greater when cattle manure was used as fertilizer, while geraniol production increased when NPK (3-12-6) was used.The lowest contents of 6,9-guaiadien were observed with the silver-colored plastic mulch using no fertilizer or cattle manure for fertilizing (Table3). The production of geranial was strongly reduced by two particular combinations: the use of a black plastic mulch with cattle manure and the use of a silver-colored plastic mulch with cattle manure + NPK 3-12-6 (Table 3). These observations highlight the negative influence of cattle manure combined with the plastic mulches on the geranial compound.Significant differences were also observed for citronellyl formate content, which was reduced when the silver-colored plastic mulch was combined with cattle manure or without fertilizer. The isomenthone content was lowest when NPK 3-12-6 was combined with no plastic mulch and when cattle manure was combined with the black plastic mulch.


## 4. Discussion

The present evaluation of different colored plastic mulches demonstrates the effectiveness of mulching for geranium production in light of the fact that the control treatment (without mulch) resulted in lower values for almost all of the agronomic variables assessed. Plant development is certainly influenced by factors affected by the use of plastic mulch, such as moisture retention, reduced competition for light and nutrients due to the absence of weeds, a reduction of pests, and an increase in the surface temperature of the soil [26].The findings of similar studies confirm these benefits, including the study of [27], which demonstrated a reduction in the productivity of cantaloupe when no plastic mulch was used. In experiments investigating the use of plastic mulch on potato crops, plants were found to emerge earlier, achieving greater height and tuber volume, when a plastic mulch was used on the soil [28, 29]. In a study assessing five different plastic mulch colors (black, white, red, silver, and blue) on an okra crop, the greatest yield was obtained with the silver-colored plastic mulch, followed by the blue plastic mulch, while the control treatment with no mulch produced the lowest yield [30].Moreover, studies evaluating the effects of other factors associated with soil coverage, such as changes in the soil microclimate and the incidence of agricultural pests, demonstrate how the use of plastic mulch can be advantageous and economical for producers [31–33].InMelissa officinalis L. soil coverage, with either pine needles or black plastic mulch, improved the biomass and essential oil yields [34]. In a related study in patchouli (Pogostemon cablin(Blanco) Benth.), the use of organic mulch for soil coverage improved the yield, amount, and quality of the patchouli essential oil [35]. InP. graveolens, an absence of soil coverage was associated with reduced yield and essential oil content along with changes in the contents of the major components of the essential oil [18]. In the present study, different fertilizer treatments did not influence the cultivation of geranium; this finding is probably due to the low nutritional requirements of the crop and its tolerance of acidic soils. It is likely that the existing nutrients in the soil sufficiently supported the crop during the evaluation period. On the other hand, when different levels of nitrogen fertilizer were tested inP. graveolens, significant differences were observed for the biomass and essential oil yield and content [18]. In a related study inOcimum basilicum L. the use of organic-mineral fertilizer did not significantly influence the development of the plants or the production of essential oil [36]. Gopichand et al. [37] similarly observed that different levels of organic fertilizer did not significantly affect the development, yield, and essential oil content ofCurcuma aromatica.The results of the present study suggest that the interaction observed among the various factors and the changes in the contents of the major components of the geranium essential oil were due to the benefits provided by the plastic mulch: the temperature and humidity of the soil were maintained, and the resources necessary for the biosynthesis of secondary metabolites were available to the plants. The high contents of the essential oil and its major components in treatments where a plastic cover was used are in accordance with the data found in the literature.


## 5. Conclusions

The absence of a soil cover negatively influenced the agronomical variables, while coverage with plastic mulch was associated with increased biomass. When cattle manure and NPK 3-12-6 were used together, combined with white or black plastic mulch, the highest yields of essential oil were obtained. For the silver-colored plastic mulch, higher amounts of essential oil (6,9-guaiadien) were obtained with mineral fertilizer.



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*Source: 828259-2014-03-16.xml*