# Binding Quantum Dots to Silk Biomaterials for Optical Sensing

Authors: Disi Lu; Zhaozhu Zheng; Shaozhe Guo; Cheng Wang; David L. Kaplan; Xiaoqin Wang
Journal: Journal of Sensors
 (2015)

---

## Abstract

Quantum dots (QDs), have great potential for fabricating optical sensing devices and
imaging biomaterial degradationin vivo. In the present study, 2-mercaptoethylamine- (MEA-)
and mercaptopropionic acid- (MPA-) capped CdTe-QDs were physically incorporated in silk films that contained
a high content (>30%) of crystalline beta-sheet structure. The beta-sheets were induced by the
addition of glycerol, water annealing, glycerol/annealing, or treatment with methanol. Incorporation of QDs did
not influence the formation of beta-sheets. When the films were extracted with water, most QDs remained
associated with the silk, based on the retention of photoluminescence in the silk films and negligible
photoluminescence in the extracts. Compared to the solution state, photoluminescence intensity significantly decreased
for MEA-QDs but not for MPA-QDs in the silk films, while the emission maximum blue shifted (≈4 nm)
slightly for both. Further film digestion using protease XIV, alpha-chymotrypsin, and the combination of the two proteases
suggested that QDs may be bound to the silk beta-sheet regions but not the amorphous regions.
QDs photoluminescence in silk films was quenched when the concentration of hydrogen peroxide
(H2O2) was above 0.2-0.3 mM, indicating the QDs-incorporated
silk films can be used to report oxidation potential in solution.


---

## Body

## 1. Introduction

Quantum dot (QD) is a type of synthetic nanocrystal composed of an inorganic core and an organic outer layer of surfactant molecules (ligands). Compared with organic dyes, QDs possess greater brightness, better stability with respect to photobleaching, and narrower spectral line widths [1], thus having potential for optoelectronic and photovoltaic devices, optical amplifier media, and bioimaging [2]. QDs have been used as photoluminescence probes to detect many analytes, such as hydrogen ions (pH), metal ions, organic compounds, and biomolecules in a growing range of chemical and bioanalytical applications [3–5]. ODs have also been used in glucose oxidase (GOX-) based glucose monitoring; for example, photoluminescence of thioglycolic acid- (TGA-) capped CdTe-QDs was quenched in response to the change of H2O2 and pH [3, 6, 7]. Thein vivo uses of QDs have been hindered mainly due to toxicity and potential risks to the environment [8]. Various modifications have been employed to improve the stability and safety of QDs forin vivo applications, including surface modifications, coatings, and encapsulation in carriers [9].Assembling QDs on solid surfaces with minimal influence of photoluminescence is critical for their integration with solid-state devices. Subtle interactions between the surface atoms of QDs and surrounding molecules can significantly influence the photoluminescence characteristics of QDs. Several fabrication techniques are used to make ultrathin organized nanocomposite films for the incorporation of QDs, including spin casting, Langmuir-Blodgett (LB) deposition, and layer-by-layer (LbL) assembly [2, 10, 11]. However, there are challenges to maintain the photoluminescence stability of QDs in the solid state: (i) the QD photoluminescence quantum yields can be significantly reduced via solvent transfer steps usually involved in fabrication procedures. (ii) QD photoluminescence quantum yields can rapidly deteriorate upon continuous excitation in solid state as a function of moisture, oxygen, heat, and light, worse than in solution phase where an excess of surface ligands passivate the QD surface. (iii) Optical spectra can be red-shifted or blue-shifted due to QD aggregation or degradation, respectively, during material solidification process [12]. Aside from coating with amphiphilic molecules, such as phospholipids, polymers, and saccharides [13–15], one effective method to stabilize QDs is to incorporate them into an appropriate host matrix such as organic polymers, carbon nanomaterials, and inorganic materials throughin situ nanocrystal growth, electrostatic interactions, and cross-linking processes [16–18]. To achieve these improvements, the original procedures for material synthesis need to be modified or reoptimized, which is not practical in most applications.Silks spun by spiders and silkworms represent the strongest and toughest natural fibers known and offer unlimited opportunities for functionalization, processing, and biological integration [19]. Silk fibroin protein isolated fromBombyx mori silkworm cocoons can be used directly for surface coatings or the fabrication of various biomaterials (films, hydrogels, sponges, fibers, and particles), thus holding promise in biomedical applications ranging from tissue engineering to controlled drug delivery due to the unique structural and biological properties [19]. Long-term stabilization and sustained release of bioactive molecules from silk biomaterials have been well documented [20, 21]. Other advantages of using silk as biomaterials include solvent-free processing and sterilization by autoclaving or gamma irradiation [22]. Silk films have been widely studied and can be prepared by drying silk solution in the air and further treated by water vapor or alcohol to increase content of thermodynamically stable beta-sheets (over 50% of total secondary structures of fibroin) and thus crystallinity [22]. No chemical cross-linking reactions or postprocessing cross-linking is needed during these processes. Through fine-tuning beta-sheet content, such as time- and temperature-dependent water annealing, mechanically robust films with tunable thicknesses (from below ten nanometers to hundreds of micrometers or more), high surface quality (surface roughness about 5 nm RMS) and transparency (over 90% transmission across the visible range) can be obtained, which are suitable for optical and electronic substrate uses [23].In a previous study we showed that 3-mercaptopropionic acid- (MPA-) coated CdTe-QDs could be incorporated in silk hydrogels and microspheres with minimal impact on photoluminescence properties and silk structures (to be published). In the present study, the interaction between two different types of QDs (MPA-capped and 2-mercaptoethylamine- (MEA-) capped) and silk was further investigated by monitoring photoluminescence changes under various conditions, such as different film treatments, proteases digestion, and oxygen peroxide (H2O2) quenching. The results elucidated the mechanism of QDs binding to silk and demonstrated the potential of using QDs-bound silk biomaterials in biosensing but also for tracking thein vivo degradation of silk biomaterials.


## 2. Materials and Methods

### 2.1. Materials

Partially degummed silk fibers were purchased from Xiehe Silk Corporation Ltd. (Hangzhou, China). Lithium bromide (LiBr) was purchased from Aladdin (Shanghai, China). Cd(ClO4)2·6H2O, Al2Te3 powders, 2-mercaptoethylamine (MEA), and mercaptopropionic acid (MPA) were purchased from Alfa Aesar (MA, USA). Protease XIV and alpha-chymotrypsin were purchased from Sigma-Aldrich. Analytical grade hydrogen peroxide (H2O2) and other chemical reagents were all purchased from Sinopharm Chemical Reagent Corporation Ltd. (Shanghai, China).


### 2.2. Methods

#### 2.2.1. Synthesis of CdTe-QDs

The CdTe-QDs were prepared by colloidal methods as reported in the literature [24, 25]. Briefly, 0.985 g (2.35 mmol) of Cd(ClO4)2·6H2O was added to 125 mL water. To this, 5.7 mmol MPA or MEA was added. The pH of the above solution was adjusted to 11.2 or 5.6 and purged with N2. The tellurium precursor H2Te gas was prepared by reaction of 0.2 g (0.46 mmol) of Al2Te3 lumps with 15–20 mL of 0.5 M H2SO4 under N2 atmosphere. H2Te gas was passed through the solution together with a slow nitrogen flow for ~20 min. CdTe precursors were formed at this stage as seen by a change of the solution color. The precursors were converted to CdTe nanocrystals by refluxing the reaction mixture at 100°C. The size of the CdTe-QDs grew further with time and was controlled by the duration of reflux, which was monitored by photoluminescence spectroscopy.


#### 2.2.2. Purification of Silk Fibroin

Silk fibroin was purified by degumming in sodium carbonate solution and dissolving in lithium bromide solution as reported in the literature [22]. Briefly, silk fibers were boiled in 0.02 M sodium carbonate solution for 30 min, rinsed with ultrapure water three times, drained, and dried in a fume hood overnight. The dried fibers were dissolved in 9.3 M lithium bromide solution at 60°C for 4 h to obtain a concentration of 20%. The solution was dialyzed against pure water for 30 h to remove the lithium bromide and centrifuged to remove insoluble fibrous debris. The concentration of purified silk was about 8-9 wt.%.


#### 2.2.3. Incorporation of QDs in Silk Films

QDs were incorporated in silk films that were prepared following standard protocols [22]. Briefly, synthesized QDs (31.5 mM) were added to 4% silk solution. The mixed solution was aliquoted into a 24-well plate with 300 μL for each well, and the plate was placed in a fume hood overnight for drying. The mass of silk was approximately 12 mg in each film. The amount of MPA-QDs loaded was 0, 9.75, 19.5, 39, 52, and 78 μmol, and the amount of MEA-QDs was 0, 15.75, 31.5, 63, 84, and 126 μmol. Silk and silk/QDs films in each well were treated as follows: (1) silk solution was supplemented with 1.3% (w/v) glycerol prior film casting; (2) as-cast silk films were vacuumed overnight in a desiccator with a water tank at the bottom (water-annealing); (3) silk/glycerol films were water annealed as described above (glycerol and water-annealing); (4) films were immersed in 300 μL methanol for 1 h and then redried in the air (methanol treatment). All these treatments induced silk beta-sheet structure formation to some extents, thus making silk film water insoluble. The addition of glycerol (>30% silk mass) conferred water-insolubility to silk films and also made the films more flexible and smooth than methanol-treated and water-annealed films [26].


#### 2.2.4. Photoluminescence Measurement and Photography

Photoluminescence spectra were recorded using a synergy H1 microplate reader (Bio-Tek, USA) at excitation wavelength of 380 nm and emission wavelength of 450–700 nm, in black 96-well plates (Conning). If not specifically stated, the top probe was set with a vertical offset of 7.00 mm and sensitivity of 100. Photographs were taken by a Cannon 60D digital camera.


#### 2.2.5. Silk Structure Determination by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR)

Silk films with and without encapsulation of QDs were loaded into an ATR-FTIR instrument (Nicolet 5700, Thermo Fisher Scientific Inc., America) with resolution of 4 cm−1, scanning range from 400 to 4000 cm−1, and 64 scans in total. The peaks shown at 1616–1637 cm−1 and 1695–1705 cm−1 were assigned to silk β-sheet structure, 1638–1655 cm−1 to random coil structure, 1656–1663 cm−1 to α-helical structure, and 1663–1695 cm−1 to β-turn structure [27]. To identify the proportion of beta-sheet structure among all secondary structures of silk, the absorbance peaks in the Amide I region (1595–1705 cm−1) were deconvoluted using Peakfit software with three repeats of data fitting for each sample (N=3).


#### 2.2.6. Proteolytic Degradation

The QDs-loaded silk films were treated with protease XIV, alpha-chymotrypsin, and the combination of protease XIV and alpha-chymotrypsin (1/1 weight ratio) in 0.1 M phosphate buffer, pH 7.4. Silk films formed in 24 wells were immersed in 1 mL of freshly prepared protease solution (300μg/mL). After incubation at 37°C for 24 h, the solution containing noncleavable silk particles (precipitate) was removed to an empty tube. Silk films without exposure to proteases (controls), residual film debris after removal of solution, and digested solution were photographed under a UV light with excitation wavelength of 365 nm.


#### 2.2.7. QDs Photoluminescence Quenching by Hydrogen Peroxide (H2O2)

QDs-loaded silk films were prepared as described above in a 24-well plate. Each film was immersed in 900μL PBS buffer, pH 7.4. After incubation for 5 min, 100 μL H2O2 solution with concentrations of 0.01, 0.05, 0.1, 0.5, 0.75, 1, 2, 3, 3.5, 4, 4.5, 5, 6.25, 7.5, 8.75, 10, 25, 50, 75, and 100 mM (diluted from 10 M H2O2 stock solution) was added to each well to start reaction. In the control wells 100 μL ultrapure water instead of H2O2 was added. The plate was placed at 37°C and incubated for 15 min before being photographed under the UV light. The films were then removed from the wells and cut into small pieces with a defined size and weight, which were placed into black 96-well plates for photoluminescence measurement. To compare photoluminescence intensity changes in response to H2O2, the photoluminescence maximum for each sample was normalized by the photoluminescence intensity in the control sample. Four repeat samples (N=4) were measured for each H2O2 concentration.


#### 2.2.8. Statistics

Results were expressed as means ± standard deviations. Statistical differences between samples were evaluated in SPSS (16.0) using one-way ANOVA test. Difference was considered significant whenp<0.05.


## 2.1. Materials

Partially degummed silk fibers were purchased from Xiehe Silk Corporation Ltd. (Hangzhou, China). Lithium bromide (LiBr) was purchased from Aladdin (Shanghai, China). Cd(ClO4)2·6H2O, Al2Te3 powders, 2-mercaptoethylamine (MEA), and mercaptopropionic acid (MPA) were purchased from Alfa Aesar (MA, USA). Protease XIV and alpha-chymotrypsin were purchased from Sigma-Aldrich. Analytical grade hydrogen peroxide (H2O2) and other chemical reagents were all purchased from Sinopharm Chemical Reagent Corporation Ltd. (Shanghai, China).


## 2.2. Methods

### 2.2.1. Synthesis of CdTe-QDs

The CdTe-QDs were prepared by colloidal methods as reported in the literature [24, 25]. Briefly, 0.985 g (2.35 mmol) of Cd(ClO4)2·6H2O was added to 125 mL water. To this, 5.7 mmol MPA or MEA was added. The pH of the above solution was adjusted to 11.2 or 5.6 and purged with N2. The tellurium precursor H2Te gas was prepared by reaction of 0.2 g (0.46 mmol) of Al2Te3 lumps with 15–20 mL of 0.5 M H2SO4 under N2 atmosphere. H2Te gas was passed through the solution together with a slow nitrogen flow for ~20 min. CdTe precursors were formed at this stage as seen by a change of the solution color. The precursors were converted to CdTe nanocrystals by refluxing the reaction mixture at 100°C. The size of the CdTe-QDs grew further with time and was controlled by the duration of reflux, which was monitored by photoluminescence spectroscopy.


### 2.2.2. Purification of Silk Fibroin

Silk fibroin was purified by degumming in sodium carbonate solution and dissolving in lithium bromide solution as reported in the literature [22]. Briefly, silk fibers were boiled in 0.02 M sodium carbonate solution for 30 min, rinsed with ultrapure water three times, drained, and dried in a fume hood overnight. The dried fibers were dissolved in 9.3 M lithium bromide solution at 60°C for 4 h to obtain a concentration of 20%. The solution was dialyzed against pure water for 30 h to remove the lithium bromide and centrifuged to remove insoluble fibrous debris. The concentration of purified silk was about 8-9 wt.%.


### 2.2.3. Incorporation of QDs in Silk Films

QDs were incorporated in silk films that were prepared following standard protocols [22]. Briefly, synthesized QDs (31.5 mM) were added to 4% silk solution. The mixed solution was aliquoted into a 24-well plate with 300 μL for each well, and the plate was placed in a fume hood overnight for drying. The mass of silk was approximately 12 mg in each film. The amount of MPA-QDs loaded was 0, 9.75, 19.5, 39, 52, and 78 μmol, and the amount of MEA-QDs was 0, 15.75, 31.5, 63, 84, and 126 μmol. Silk and silk/QDs films in each well were treated as follows: (1) silk solution was supplemented with 1.3% (w/v) glycerol prior film casting; (2) as-cast silk films were vacuumed overnight in a desiccator with a water tank at the bottom (water-annealing); (3) silk/glycerol films were water annealed as described above (glycerol and water-annealing); (4) films were immersed in 300 μL methanol for 1 h and then redried in the air (methanol treatment). All these treatments induced silk beta-sheet structure formation to some extents, thus making silk film water insoluble. The addition of glycerol (>30% silk mass) conferred water-insolubility to silk films and also made the films more flexible and smooth than methanol-treated and water-annealed films [26].


### 2.2.4. Photoluminescence Measurement and Photography

Photoluminescence spectra were recorded using a synergy H1 microplate reader (Bio-Tek, USA) at excitation wavelength of 380 nm and emission wavelength of 450–700 nm, in black 96-well plates (Conning). If not specifically stated, the top probe was set with a vertical offset of 7.00 mm and sensitivity of 100. Photographs were taken by a Cannon 60D digital camera.


### 2.2.5. Silk Structure Determination by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR)

Silk films with and without encapsulation of QDs were loaded into an ATR-FTIR instrument (Nicolet 5700, Thermo Fisher Scientific Inc., America) with resolution of 4 cm−1, scanning range from 400 to 4000 cm−1, and 64 scans in total. The peaks shown at 1616–1637 cm−1 and 1695–1705 cm−1 were assigned to silk β-sheet structure, 1638–1655 cm−1 to random coil structure, 1656–1663 cm−1 to α-helical structure, and 1663–1695 cm−1 to β-turn structure [27]. To identify the proportion of beta-sheet structure among all secondary structures of silk, the absorbance peaks in the Amide I region (1595–1705 cm−1) were deconvoluted using Peakfit software with three repeats of data fitting for each sample (N=3).


### 2.2.6. Proteolytic Degradation

The QDs-loaded silk films were treated with protease XIV, alpha-chymotrypsin, and the combination of protease XIV and alpha-chymotrypsin (1/1 weight ratio) in 0.1 M phosphate buffer, pH 7.4. Silk films formed in 24 wells were immersed in 1 mL of freshly prepared protease solution (300μg/mL). After incubation at 37°C for 24 h, the solution containing noncleavable silk particles (precipitate) was removed to an empty tube. Silk films without exposure to proteases (controls), residual film debris after removal of solution, and digested solution were photographed under a UV light with excitation wavelength of 365 nm.


### 2.2.7. QDs Photoluminescence Quenching by Hydrogen Peroxide (H2O2)

QDs-loaded silk films were prepared as described above in a 24-well plate. Each film was immersed in 900μL PBS buffer, pH 7.4. After incubation for 5 min, 100 μL H2O2 solution with concentrations of 0.01, 0.05, 0.1, 0.5, 0.75, 1, 2, 3, 3.5, 4, 4.5, 5, 6.25, 7.5, 8.75, 10, 25, 50, 75, and 100 mM (diluted from 10 M H2O2 stock solution) was added to each well to start reaction. In the control wells 100 μL ultrapure water instead of H2O2 was added. The plate was placed at 37°C and incubated for 15 min before being photographed under the UV light. The films were then removed from the wells and cut into small pieces with a defined size and weight, which were placed into black 96-well plates for photoluminescence measurement. To compare photoluminescence intensity changes in response to H2O2, the photoluminescence maximum for each sample was normalized by the photoluminescence intensity in the control sample. Four repeat samples (N=4) were measured for each H2O2 concentration.


### 2.2.8. Statistics

Results were expressed as means ± standard deviations. Statistical differences between samples were evaluated in SPSS (16.0) using one-way ANOVA test. Difference was considered significant whenp<0.05.


## 2.2.1. Synthesis of CdTe-QDs

The CdTe-QDs were prepared by colloidal methods as reported in the literature [24, 25]. Briefly, 0.985 g (2.35 mmol) of Cd(ClO4)2·6H2O was added to 125 mL water. To this, 5.7 mmol MPA or MEA was added. The pH of the above solution was adjusted to 11.2 or 5.6 and purged with N2. The tellurium precursor H2Te gas was prepared by reaction of 0.2 g (0.46 mmol) of Al2Te3 lumps with 15–20 mL of 0.5 M H2SO4 under N2 atmosphere. H2Te gas was passed through the solution together with a slow nitrogen flow for ~20 min. CdTe precursors were formed at this stage as seen by a change of the solution color. The precursors were converted to CdTe nanocrystals by refluxing the reaction mixture at 100°C. The size of the CdTe-QDs grew further with time and was controlled by the duration of reflux, which was monitored by photoluminescence spectroscopy.


## 2.2.2. Purification of Silk Fibroin

Silk fibroin was purified by degumming in sodium carbonate solution and dissolving in lithium bromide solution as reported in the literature [22]. Briefly, silk fibers were boiled in 0.02 M sodium carbonate solution for 30 min, rinsed with ultrapure water three times, drained, and dried in a fume hood overnight. The dried fibers were dissolved in 9.3 M lithium bromide solution at 60°C for 4 h to obtain a concentration of 20%. The solution was dialyzed against pure water for 30 h to remove the lithium bromide and centrifuged to remove insoluble fibrous debris. The concentration of purified silk was about 8-9 wt.%.


## 2.2.3. Incorporation of QDs in Silk Films

QDs were incorporated in silk films that were prepared following standard protocols [22]. Briefly, synthesized QDs (31.5 mM) were added to 4% silk solution. The mixed solution was aliquoted into a 24-well plate with 300 μL for each well, and the plate was placed in a fume hood overnight for drying. The mass of silk was approximately 12 mg in each film. The amount of MPA-QDs loaded was 0, 9.75, 19.5, 39, 52, and 78 μmol, and the amount of MEA-QDs was 0, 15.75, 31.5, 63, 84, and 126 μmol. Silk and silk/QDs films in each well were treated as follows: (1) silk solution was supplemented with 1.3% (w/v) glycerol prior film casting; (2) as-cast silk films were vacuumed overnight in a desiccator with a water tank at the bottom (water-annealing); (3) silk/glycerol films were water annealed as described above (glycerol and water-annealing); (4) films were immersed in 300 μL methanol for 1 h and then redried in the air (methanol treatment). All these treatments induced silk beta-sheet structure formation to some extents, thus making silk film water insoluble. The addition of glycerol (>30% silk mass) conferred water-insolubility to silk films and also made the films more flexible and smooth than methanol-treated and water-annealed films [26].


## 2.2.4. Photoluminescence Measurement and Photography

Photoluminescence spectra were recorded using a synergy H1 microplate reader (Bio-Tek, USA) at excitation wavelength of 380 nm and emission wavelength of 450–700 nm, in black 96-well plates (Conning). If not specifically stated, the top probe was set with a vertical offset of 7.00 mm and sensitivity of 100. Photographs were taken by a Cannon 60D digital camera.


## 2.2.5. Silk Structure Determination by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR)

Silk films with and without encapsulation of QDs were loaded into an ATR-FTIR instrument (Nicolet 5700, Thermo Fisher Scientific Inc., America) with resolution of 4 cm−1, scanning range from 400 to 4000 cm−1, and 64 scans in total. The peaks shown at 1616–1637 cm−1 and 1695–1705 cm−1 were assigned to silk β-sheet structure, 1638–1655 cm−1 to random coil structure, 1656–1663 cm−1 to α-helical structure, and 1663–1695 cm−1 to β-turn structure [27]. To identify the proportion of beta-sheet structure among all secondary structures of silk, the absorbance peaks in the Amide I region (1595–1705 cm−1) were deconvoluted using Peakfit software with three repeats of data fitting for each sample (N=3).


## 2.2.6. Proteolytic Degradation

The QDs-loaded silk films were treated with protease XIV, alpha-chymotrypsin, and the combination of protease XIV and alpha-chymotrypsin (1/1 weight ratio) in 0.1 M phosphate buffer, pH 7.4. Silk films formed in 24 wells were immersed in 1 mL of freshly prepared protease solution (300μg/mL). After incubation at 37°C for 24 h, the solution containing noncleavable silk particles (precipitate) was removed to an empty tube. Silk films without exposure to proteases (controls), residual film debris after removal of solution, and digested solution were photographed under a UV light with excitation wavelength of 365 nm.


## 2.2.7. QDs Photoluminescence Quenching by Hydrogen Peroxide (H2O2)

QDs-loaded silk films were prepared as described above in a 24-well plate. Each film was immersed in 900μL PBS buffer, pH 7.4. After incubation for 5 min, 100 μL H2O2 solution with concentrations of 0.01, 0.05, 0.1, 0.5, 0.75, 1, 2, 3, 3.5, 4, 4.5, 5, 6.25, 7.5, 8.75, 10, 25, 50, 75, and 100 mM (diluted from 10 M H2O2 stock solution) was added to each well to start reaction. In the control wells 100 μL ultrapure water instead of H2O2 was added. The plate was placed at 37°C and incubated for 15 min before being photographed under the UV light. The films were then removed from the wells and cut into small pieces with a defined size and weight, which were placed into black 96-well plates for photoluminescence measurement. To compare photoluminescence intensity changes in response to H2O2, the photoluminescence maximum for each sample was normalized by the photoluminescence intensity in the control sample. Four repeat samples (N=4) were measured for each H2O2 concentration.


## 2.2.8. Statistics

Results were expressed as means ± standard deviations. Statistical differences between samples were evaluated in SPSS (16.0) using one-way ANOVA test. Difference was considered significant whenp<0.05.


## 3. Results and Discussion

### 3.1. Physical Characterization of QDs-Loaded Silk Films

MPA- and MEA-capped QDs were synthesized as described in Section2. Depending on the size, the fluorescence emissions of QDs were different (from green to red) (Figure 1). The red fluorescent QDs (diameter approximately 4.5 nm according to the literature [24, 25]) were chosen for the current study. When MPA- and MEA-QDs were mixed with silk/glycerol (3/1 w/w) solution and the mixture was air-dried in the fume hood, both MPA-silk and MEA-silk films fluoresced under UV light. Photoluminescence increased with the increase of MPA-QDs and MEA-QDs concentrations in the films (Figure 1). The films containing 39 μmol MPA-QDs and 63 μmol MPA-QDs (the 4th one from left to right on Figure 1) showed stronger photoluminescence than those with less QDs loading (to the left) while showing less difference from those with more QDs loading (to the right). The loading conditions were thus used for the following studies.Figure 1
QDs-loaded silk films exposed to UV light (excitation wavelength at 365 nm). Upper photographs showed as-prepared QDs in solution and encapsulated in silk/glycerol (weight ratio 3/1) films. Lower spectra showed the photoluminescence of as-prepared QDs in water, 4% silk (SF) solution, and silk/glycerol (SF) film, using QDs-free SF solution and SF film as controls. (a) MPA-capped CdTe-QDs. The mass of silk was 12 mg in each film, and the amount of QDs loaded was (from left to right) 0, 9.75, 19.5, 39, 52, and 78μmol. (b) MEA-capped CdTe-QDs. The mass of silk was 12 mg in each film, and the amount of QDs loaded was (from left to right) 0, 15.75, 31.5, 63, 84, and 126 μmol.
(a)
(b)The emission maximum of MPA-QDs was at 635 nm when diluted in water and in silk solution and 631 nm when dried in the silk/glycerol film. The photoluminescence maximum of MEA-QDs was at 616, 612, and 610 nm when diluted in water, silk solution, and silk/film, respectively (Figure1). Thus, MPA-QDs were more stable than MEA-QDs in terms of photoluminescence. QDs-incorporated silk films were treated in different ways (glycerol addition, water-annealing, glycerol with water-annealing, and methanol treatment) and washed with water to determine QDs release from the films. Both MPA-QDs and MEA-QDs bound tightly to silk, as seen by almost unchanged photoluminescence from the silk films after washing (Figure 2, upper photographs) and low levels of photoluminescence in washing solution determined by photoluminescence spectroscopy (Figure 2, lower graphs). The high peaks seen in the graphs were actually from the intrinsic fluorescence from silk fibroin protein; the photoluminescence from QDs at 620–630 nm was negligible even in the first washing solution (see arrows in the graphs). For MEA-QDs, neither the silk nor QDs photoluminescence was detectable in the washing solution. The fact that QDs can tightly bind to silk with minimal change of photoluminescence properties (intensity, emission maximum) suggests that the complications of cross-linking or crystal growth during processing can be avoided. Thus the QDs can be easily incorporated in silk materials via physical binding, facilitating the fabrication of silk-based optical sensors and other applications.Figure 2
Binding of MPA-QDs and MEA-QDs to silk. (a) MPA-QDs-loaded silk films after various treatments were washed with water. (b) MEA-QDs-loaded silk films after various treatments were washed with water. (c) Photoluminescence spectra of the solutions collected from washing MPA-QDs-loaded silk films. (d) Photoluminescence spectra of five washing solutions from the methanol-treated MPA-QDs-loaded silk films.
(a)
(b)
(c)
(d)


### 3.2. Mechanism of QDs Binding to Silk

Silk has been known to predominately consist of repeating hexapeptides GAGAGS and GAGAGY, which form crystalline beta-sheet structure in silk materials (e.g., films) after various treatments mentioned above, conferring strength and chemical and physical stabilities to silk materials [19]. Previous studies have shown that many types of molecules, from low molecular weight compounds to proteins, bind to silk, likely due to intermolecular interaction between the molecules and silk beta-sheet structure domains, along with the amphipathic characteristics [20, 21]. The same mechanism might also apply to QDs, as the hydrophobic core of QDs might interact with the hydrophobic beta-sheet domains in silk. When the secondary structure of silk fibroin protein was examined by ATR-FTIR, it was found that all the QDs-incorporated silk films after treatments exhibited characteristic peaks at 1616–1637 cm−1 in Amide I region, corresponding to beta-sheet structure, whereas the untreated silk film (as-cast) showed a peak at 1652 cm−1, corresponding to random coil structure (Figure 3). Further calculation of beta-sheet structure content by peak deconvolution indicated that the beta-sheet structure content in glycerol-blended, water-annealed, and glycerol-blended/water annealed films was 20–30% of the total structure, whereas it was 30–40% and less than 20% for the methanol-treated and untreated films, respectively (Figure 4). Compared to other treatment methods, methanol-treated films showed significantly higher beta-sheet structure content, consistent with the literature [27]. Compared with plain silk films (no QDs), incorporation of MPA-QDs and MEA-QDs in silk films did not result in significantly more beta-sheet structures except for the glycerol-blended/water-annealed MEA-QDs-incorporated silk films and methanol-treated MPA- and MEA-QDs films (Figure 4).Figure 3
ATR-FTIR spectra of the as-prepared QDs, silk films, and QDs-loaded silk films. (a) MPA-QDs-loaded silk film. (b) MEA-QDs-loaded silk film.
(a)
(b)Figure 4
Determination ofβ-sheet structure contents in silk films by deconvolution of the Amide I peaks (1595–1705 cm−1) on ATR-FTIR spectra. Significant difference between samples was indicated by asterisk (p<0.05).Silk materials could be digested by different proteases. Protease XIV cleaved both random coil and crystalline beta-sheet regions in silk, more efficiently for the random coil than the beta-sheet regions, while alpha-chymotrypsin only cleaved the random coil regions [28, 29]. Upon alpha-chymotrypsin digestion, the noncleavable hydrophobic beta-sheet domains formed water-insoluble precipitates. MPA-QDs-incorporated glycerol-blended silk films were used in this protease study. After alpha-chymotrypsin digestion, the solution was removed to an empty tube, in which the noncleavable silk particles precipitated and strongly photoluminesced at the bottom of the tube, while the supernatant was not photoluminescent, indicating the QDs were still bound to the crystalline beta-sheets (2nd row of photos in Figure 5). In the case of protease XIV (1st row of photos in Figure 5), the photoluminescence from the precipitate was weaker compared to that of alpha-chymotrypsin, suggesting that part of the crystalline beta-sheets was digested, releasing QDs into the supernatant. When dual proteases were used, the films were completely digested, with no visible precipitate in the tube (Figure 5, 3rd row of photos). Both amorphous and crystalline beta-sheet regions of silk were digested, resulting in the release of the bound QDs in this case. The photoluminescence spectra for the supernatants were taken using a photoluminescence spectrometer. As shown in Figure 5 (lower graph), the emission maxima of the supernatants after protease digestion blue-shifted from 631 to 621 nm. Photoluminescence intensities in the supernatants were also significantly lower than that of control QDs at the same concentration. Thus, protease digestion might have influenced the size and photoluminescence of QDs after they were released from the bound silk matrix. Photoluminescence intensity of the supernatant after protease XIV digestion was higher than that after alpha-chymotrypsin digestion, suggesting protease XIV digestion released more QDs from silk films, consistent with the photographic observations.Figure 5
Proteolytic degradation of MPA-QDs-loaded silk films by protease XIV, alpha-chymotrypsin, and the combination of protease XIV and alpha-chymotrypsin. Upper photographs from the left to the right: control (no proteases), residual silk films after removal of digested solution, and digested solution in a tube. The samples were exposed to the UV light with an excitation wavelength at 365 nm. Lower graph: photoluminescence spectra of the supernatants after removal of noncleavable silk precipitates at the bottom of the tubes.The mechanism underlying interaction of QDs with silk is schematically presented in Figure6. Silk fibroin extracted fromB. moriis composed of three structural components (heavy chain, light chain, and P25). The heavy chain, which is the key component to determine material properties, consists of the hydrophilic regions (nonrepetitive N- and C-terminus and spacer) and hydrophobic, repeating, large hexapeptide domains (GAGAGS, GAGAGY) [19]. Depending on the processing, the repetitive hexapeptide domains can form different contents of crystalline beta-sheet regions, conferring tunable strength and stability to silk biomaterials. Various types of compounds, from small molecules to peptides and proteins, can bind to silk via different mechanisms, including hydrogen bonding, electrostatic interaction, and hydrophobic interaction [20, 21]. In the present study, binding of QDs to the crystalline beta-sheet regions on silk without significant change of photoluminescence properties was demonstrated. Of note, although only methanol treatment to induce silk crystalline beta-sheet structure was indicated in the process, other treatments, for example, glycerol addition, water-annealing, and combinations of both can also induce the beta-sheet structures to lesser degrees, as indicated in the ATR-FTIR study. Thus, the mechanism described should apply to all of the silk films prepared with the different treatments.Figure 6
Schematic illustration of proteolytic degradation of QDs-loaded silk films. Crystalline beta-sheet regions in silk films were induced after methanol treatment (a). Silk crystalline beta-sheet regions were partially cleaved into nanofibril and nanofilaments by protease XIV (b), while they remained intact in the presence of alpha-chymotrypsin (c). The random coil regions of silk molecules were cleaved into soluble fragments by both protease XIV and alpha-chymotrypsin. Beta-sheet and random coil regions were both cleaved by the dual proteases (protease XIV and alpha-chymotrypsin), yielding more soluble fragments in solution (d).


### 3.3. H2O2-Induced Photoluminescence Quenching of QDs-Silk Films

To demonstrate that QDs-incorporated silk films can be used for optical sensing in biomedical applications, the response of QDs photoluminescence to hydrogen peroxide (H2O2) in solution was examined. MPA-QDs- and MEA-QDs-incorporated silk films with glycerol addition and methanol treatment were used in the study. H2O2 was added to the wells containing different films and PBS buffer, pH 7.4, to obtain a concentration range of 0.001–10 mM. The samples were photographed after reaction for 15 min at 37°C (Figures 7(A) and 7(B)). Both glycerol-added and methanol-treated films showed significant photoluminescence quenching when the H2O2 concentration reached 0.02–0.03 mM (2nd-3rd well in the second row). The films were subjected to quantitative photoluminescence determination (Figure 7(C)). Compared to the initial photoluminescence (0 mM H2O2), the photoluminescence intensity decreased approximately 40%, 75%, 45%, and 65% for MEA-QDs/glycerol film, MPA-QDs/glycerol film, MEA-QDs/methanol film, and MPA-QDs/methanol film, respectively, when the concentration of H2O2 was above 0.2 mM, confirming the photographic observations. Furthermore, it seems the glycerol-added films were more sensitive than the methanol-treated films and MPA-QDs were more sensitive than MEA-QDs to the H2O2 quenching. For both types of films, the decrease of photoluminescence plateaued after H2O2 concentration reached 2 mM (Figure 7(C)), indicating that all exposed QDs had been quenched at this point. Unquenchable QDs might be those deeply embedded in the silk matrix, likely the hydrophobic beta-sheet stacks, which could not be penetrated by the H2O2 molecules.Figure 7
Change of photoluminescence intensities in QDs-loaded silk films in response to H2O2. (a) shows glycerol-added silk films. (b) shows methanol-treated silk films. (A) MPA-QDs-loaded silk films. (B) MEA-QDs-loaded silk films. H2O2 concentrations in the samples (from left to right): 0.0, 0.001, 0.005, 0.01, 0.05, and 0.075 mM on the 1st row; 0.1, 0.2, 0.3, 0.35, 0.4, and 0.45 mM on the 2nd row; 0.5, 0.625, 0.75, 0.875, 1.0, and 2.5 mM on the 3rd row; 5.0, 7.5, 10.0, and 0.0 mM on the 4th row. (C) Photoluminescence intensity changes as a function of H2O2 concentrations in solution.
(a)
(b)H2O2 is generated during the glucose oxidase (GOD) catalyzed reaction of βD-glucose and oxygen and has been used as an indicator for glucose level in diabetes [30]. H2O2 can also be generated during pathogenic biological processes, for example, cancer, stroke, renal failure, anathema, and chronic obstructive pulmonary disease (COPD), and thus has been used as an indicator forin vitro andin vivo diagnosis [31]. Due to its low concentrations (<0.1 μm), H2O2 had to be condensed from the exhaled breath prior to electrochemical or colorimetrical assays. Recent progress on nanomaterials has significantly improved the sensitivity and accuracy of H2O2 detection [32]. The present study provides another option for fabricating H2O2 detection devices using QDs-incorporated silk materials. Due to the natural sourcing and “green” processing of silk materials, along with the strong binding of QDs to silk, QDs-incorporated silk-based optical sensors could find broad applications in biomedical and environment applications. Except for optical sensing, the physical incorporation of QDs can also be used for tracking degradation and thus the durability of silk-based biomaterials after implantation or injectionin vivo.


## 3.1. Physical Characterization of QDs-Loaded Silk Films

MPA- and MEA-capped QDs were synthesized as described in Section2. Depending on the size, the fluorescence emissions of QDs were different (from green to red) (Figure 1). The red fluorescent QDs (diameter approximately 4.5 nm according to the literature [24, 25]) were chosen for the current study. When MPA- and MEA-QDs were mixed with silk/glycerol (3/1 w/w) solution and the mixture was air-dried in the fume hood, both MPA-silk and MEA-silk films fluoresced under UV light. Photoluminescence increased with the increase of MPA-QDs and MEA-QDs concentrations in the films (Figure 1). The films containing 39 μmol MPA-QDs and 63 μmol MPA-QDs (the 4th one from left to right on Figure 1) showed stronger photoluminescence than those with less QDs loading (to the left) while showing less difference from those with more QDs loading (to the right). The loading conditions were thus used for the following studies.Figure 1
QDs-loaded silk films exposed to UV light (excitation wavelength at 365 nm). Upper photographs showed as-prepared QDs in solution and encapsulated in silk/glycerol (weight ratio 3/1) films. Lower spectra showed the photoluminescence of as-prepared QDs in water, 4% silk (SF) solution, and silk/glycerol (SF) film, using QDs-free SF solution and SF film as controls. (a) MPA-capped CdTe-QDs. The mass of silk was 12 mg in each film, and the amount of QDs loaded was (from left to right) 0, 9.75, 19.5, 39, 52, and 78μmol. (b) MEA-capped CdTe-QDs. The mass of silk was 12 mg in each film, and the amount of QDs loaded was (from left to right) 0, 15.75, 31.5, 63, 84, and 126 μmol.
(a)
(b)The emission maximum of MPA-QDs was at 635 nm when diluted in water and in silk solution and 631 nm when dried in the silk/glycerol film. The photoluminescence maximum of MEA-QDs was at 616, 612, and 610 nm when diluted in water, silk solution, and silk/film, respectively (Figure1). Thus, MPA-QDs were more stable than MEA-QDs in terms of photoluminescence. QDs-incorporated silk films were treated in different ways (glycerol addition, water-annealing, glycerol with water-annealing, and methanol treatment) and washed with water to determine QDs release from the films. Both MPA-QDs and MEA-QDs bound tightly to silk, as seen by almost unchanged photoluminescence from the silk films after washing (Figure 2, upper photographs) and low levels of photoluminescence in washing solution determined by photoluminescence spectroscopy (Figure 2, lower graphs). The high peaks seen in the graphs were actually from the intrinsic fluorescence from silk fibroin protein; the photoluminescence from QDs at 620–630 nm was negligible even in the first washing solution (see arrows in the graphs). For MEA-QDs, neither the silk nor QDs photoluminescence was detectable in the washing solution. The fact that QDs can tightly bind to silk with minimal change of photoluminescence properties (intensity, emission maximum) suggests that the complications of cross-linking or crystal growth during processing can be avoided. Thus the QDs can be easily incorporated in silk materials via physical binding, facilitating the fabrication of silk-based optical sensors and other applications.Figure 2
Binding of MPA-QDs and MEA-QDs to silk. (a) MPA-QDs-loaded silk films after various treatments were washed with water. (b) MEA-QDs-loaded silk films after various treatments were washed with water. (c) Photoluminescence spectra of the solutions collected from washing MPA-QDs-loaded silk films. (d) Photoluminescence spectra of five washing solutions from the methanol-treated MPA-QDs-loaded silk films.
(a)
(b)
(c)
(d)


## 3.2. Mechanism of QDs Binding to Silk

Silk has been known to predominately consist of repeating hexapeptides GAGAGS and GAGAGY, which form crystalline beta-sheet structure in silk materials (e.g., films) after various treatments mentioned above, conferring strength and chemical and physical stabilities to silk materials [19]. Previous studies have shown that many types of molecules, from low molecular weight compounds to proteins, bind to silk, likely due to intermolecular interaction between the molecules and silk beta-sheet structure domains, along with the amphipathic characteristics [20, 21]. The same mechanism might also apply to QDs, as the hydrophobic core of QDs might interact with the hydrophobic beta-sheet domains in silk. When the secondary structure of silk fibroin protein was examined by ATR-FTIR, it was found that all the QDs-incorporated silk films after treatments exhibited characteristic peaks at 1616–1637 cm−1 in Amide I region, corresponding to beta-sheet structure, whereas the untreated silk film (as-cast) showed a peak at 1652 cm−1, corresponding to random coil structure (Figure 3). Further calculation of beta-sheet structure content by peak deconvolution indicated that the beta-sheet structure content in glycerol-blended, water-annealed, and glycerol-blended/water annealed films was 20–30% of the total structure, whereas it was 30–40% and less than 20% for the methanol-treated and untreated films, respectively (Figure 4). Compared to other treatment methods, methanol-treated films showed significantly higher beta-sheet structure content, consistent with the literature [27]. Compared with plain silk films (no QDs), incorporation of MPA-QDs and MEA-QDs in silk films did not result in significantly more beta-sheet structures except for the glycerol-blended/water-annealed MEA-QDs-incorporated silk films and methanol-treated MPA- and MEA-QDs films (Figure 4).Figure 3
ATR-FTIR spectra of the as-prepared QDs, silk films, and QDs-loaded silk films. (a) MPA-QDs-loaded silk film. (b) MEA-QDs-loaded silk film.
(a)
(b)Figure 4
Determination ofβ-sheet structure contents in silk films by deconvolution of the Amide I peaks (1595–1705 cm−1) on ATR-FTIR spectra. Significant difference between samples was indicated by asterisk (p<0.05).Silk materials could be digested by different proteases. Protease XIV cleaved both random coil and crystalline beta-sheet regions in silk, more efficiently for the random coil than the beta-sheet regions, while alpha-chymotrypsin only cleaved the random coil regions [28, 29]. Upon alpha-chymotrypsin digestion, the noncleavable hydrophobic beta-sheet domains formed water-insoluble precipitates. MPA-QDs-incorporated glycerol-blended silk films were used in this protease study. After alpha-chymotrypsin digestion, the solution was removed to an empty tube, in which the noncleavable silk particles precipitated and strongly photoluminesced at the bottom of the tube, while the supernatant was not photoluminescent, indicating the QDs were still bound to the crystalline beta-sheets (2nd row of photos in Figure 5). In the case of protease XIV (1st row of photos in Figure 5), the photoluminescence from the precipitate was weaker compared to that of alpha-chymotrypsin, suggesting that part of the crystalline beta-sheets was digested, releasing QDs into the supernatant. When dual proteases were used, the films were completely digested, with no visible precipitate in the tube (Figure 5, 3rd row of photos). Both amorphous and crystalline beta-sheet regions of silk were digested, resulting in the release of the bound QDs in this case. The photoluminescence spectra for the supernatants were taken using a photoluminescence spectrometer. As shown in Figure 5 (lower graph), the emission maxima of the supernatants after protease digestion blue-shifted from 631 to 621 nm. Photoluminescence intensities in the supernatants were also significantly lower than that of control QDs at the same concentration. Thus, protease digestion might have influenced the size and photoluminescence of QDs after they were released from the bound silk matrix. Photoluminescence intensity of the supernatant after protease XIV digestion was higher than that after alpha-chymotrypsin digestion, suggesting protease XIV digestion released more QDs from silk films, consistent with the photographic observations.Figure 5
Proteolytic degradation of MPA-QDs-loaded silk films by protease XIV, alpha-chymotrypsin, and the combination of protease XIV and alpha-chymotrypsin. Upper photographs from the left to the right: control (no proteases), residual silk films after removal of digested solution, and digested solution in a tube. The samples were exposed to the UV light with an excitation wavelength at 365 nm. Lower graph: photoluminescence spectra of the supernatants after removal of noncleavable silk precipitates at the bottom of the tubes.The mechanism underlying interaction of QDs with silk is schematically presented in Figure6. Silk fibroin extracted fromB. moriis composed of three structural components (heavy chain, light chain, and P25). The heavy chain, which is the key component to determine material properties, consists of the hydrophilic regions (nonrepetitive N- and C-terminus and spacer) and hydrophobic, repeating, large hexapeptide domains (GAGAGS, GAGAGY) [19]. Depending on the processing, the repetitive hexapeptide domains can form different contents of crystalline beta-sheet regions, conferring tunable strength and stability to silk biomaterials. Various types of compounds, from small molecules to peptides and proteins, can bind to silk via different mechanisms, including hydrogen bonding, electrostatic interaction, and hydrophobic interaction [20, 21]. In the present study, binding of QDs to the crystalline beta-sheet regions on silk without significant change of photoluminescence properties was demonstrated. Of note, although only methanol treatment to induce silk crystalline beta-sheet structure was indicated in the process, other treatments, for example, glycerol addition, water-annealing, and combinations of both can also induce the beta-sheet structures to lesser degrees, as indicated in the ATR-FTIR study. Thus, the mechanism described should apply to all of the silk films prepared with the different treatments.Figure 6
Schematic illustration of proteolytic degradation of QDs-loaded silk films. Crystalline beta-sheet regions in silk films were induced after methanol treatment (a). Silk crystalline beta-sheet regions were partially cleaved into nanofibril and nanofilaments by protease XIV (b), while they remained intact in the presence of alpha-chymotrypsin (c). The random coil regions of silk molecules were cleaved into soluble fragments by both protease XIV and alpha-chymotrypsin. Beta-sheet and random coil regions were both cleaved by the dual proteases (protease XIV and alpha-chymotrypsin), yielding more soluble fragments in solution (d).


## 3.3. H2O2-Induced Photoluminescence Quenching of QDs-Silk Films

To demonstrate that QDs-incorporated silk films can be used for optical sensing in biomedical applications, the response of QDs photoluminescence to hydrogen peroxide (H2O2) in solution was examined. MPA-QDs- and MEA-QDs-incorporated silk films with glycerol addition and methanol treatment were used in the study. H2O2 was added to the wells containing different films and PBS buffer, pH 7.4, to obtain a concentration range of 0.001–10 mM. The samples were photographed after reaction for 15 min at 37°C (Figures 7(A) and 7(B)). Both glycerol-added and methanol-treated films showed significant photoluminescence quenching when the H2O2 concentration reached 0.02–0.03 mM (2nd-3rd well in the second row). The films were subjected to quantitative photoluminescence determination (Figure 7(C)). Compared to the initial photoluminescence (0 mM H2O2), the photoluminescence intensity decreased approximately 40%, 75%, 45%, and 65% for MEA-QDs/glycerol film, MPA-QDs/glycerol film, MEA-QDs/methanol film, and MPA-QDs/methanol film, respectively, when the concentration of H2O2 was above 0.2 mM, confirming the photographic observations. Furthermore, it seems the glycerol-added films were more sensitive than the methanol-treated films and MPA-QDs were more sensitive than MEA-QDs to the H2O2 quenching. For both types of films, the decrease of photoluminescence plateaued after H2O2 concentration reached 2 mM (Figure 7(C)), indicating that all exposed QDs had been quenched at this point. Unquenchable QDs might be those deeply embedded in the silk matrix, likely the hydrophobic beta-sheet stacks, which could not be penetrated by the H2O2 molecules.Figure 7
Change of photoluminescence intensities in QDs-loaded silk films in response to H2O2. (a) shows glycerol-added silk films. (b) shows methanol-treated silk films. (A) MPA-QDs-loaded silk films. (B) MEA-QDs-loaded silk films. H2O2 concentrations in the samples (from left to right): 0.0, 0.001, 0.005, 0.01, 0.05, and 0.075 mM on the 1st row; 0.1, 0.2, 0.3, 0.35, 0.4, and 0.45 mM on the 2nd row; 0.5, 0.625, 0.75, 0.875, 1.0, and 2.5 mM on the 3rd row; 5.0, 7.5, 10.0, and 0.0 mM on the 4th row. (C) Photoluminescence intensity changes as a function of H2O2 concentrations in solution.
(a)
(b)H2O2 is generated during the glucose oxidase (GOD) catalyzed reaction of βD-glucose and oxygen and has been used as an indicator for glucose level in diabetes [30]. H2O2 can also be generated during pathogenic biological processes, for example, cancer, stroke, renal failure, anathema, and chronic obstructive pulmonary disease (COPD), and thus has been used as an indicator forin vitro andin vivo diagnosis [31]. Due to its low concentrations (<0.1 μm), H2O2 had to be condensed from the exhaled breath prior to electrochemical or colorimetrical assays. Recent progress on nanomaterials has significantly improved the sensitivity and accuracy of H2O2 detection [32]. The present study provides another option for fabricating H2O2 detection devices using QDs-incorporated silk materials. Due to the natural sourcing and “green” processing of silk materials, along with the strong binding of QDs to silk, QDs-incorporated silk-based optical sensors could find broad applications in biomedical and environment applications. Except for optical sensing, the physical incorporation of QDs can also be used for tracking degradation and thus the durability of silk-based biomaterials after implantation or injectionin vivo.


## 4. Conclusions

MPA-capped and MEA-capped QDs were synthesized and physically incorporated in silk films that were treated in different ways to induce crystalline beta-sheet structure. Compared to MEA-QDs, MPA-QDs showed longer emission wavelength (>630 nm) and more stable photoluminescence properties after incorporation in silk films. Both types of QDs bound to silk tightly, with negligible amounts of QDs being extracted from the films. Protease digestion using protease XIV and alpha-chymotrypsin suggested that the QDs bound to hydrophobic beta-sheet domains preferentially in the silk matrix. The tightly bound QDs in both glycerol-added and methanol-treated silk films sensed H2O2 concentration changes in solution, with a critical concentration range of 0.2–0.3 mM, suggesting the QDs-incorporated silk materials can be used for fabricating optical sensors for diagnostic applications.



---

*Source: 819373-2015-08-16.xml*