# Comparing the Differential Diagnostic Values of18F-Alfatide II PET/CT between Tuberculosis and Lung Cancer Patients

Authors: Xiaoqing Du; Yu Zhang; Liping Chen; Baoming Mi; Qingjun You; Yuping Xu; Donghui Pan; Weixing Wan; Min Yang; Chunjing Yu
Journal: Contrast Media & Molecular Imaging
 (2018)

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## Abstract

Purpose. To compare the differential diagnostic values of 18F-Alfatide II PET/CT between tuberculosis and lung cancer patients and in patients with sarcoidosis and common inflammation.Methods. Nine inflammation patients (4 tuberculosis, 3 sarcoidosis, and 2 common inflammation) and 11 lung cancer patients were included in this study. All patients underwent 18F-FDG and 18F-Alfatide II PET/CT within 2 weeks, followed by biopsy and surgery. The maximized standard uptake value (SUVmax) and the mean standard uptake value (SUVmean) were evaluated.Results. The active tuberculosis lesions showed a high accumulation of 18F-FDG, but varying degrees of accumulation of 18F-Alfatide II, including negative results. The SUVmax of 18F-Alfatide II in malignant lesions was significantly higher than that in tuberculosis (4.08 ± 1.51 versus 2.63 ± 1.34, P = 0.0078). Three patients with sarcoidosis showed negative results in 18F-Alfatide II PET/CT.Conclusions. The expression of αVβ3 is much lower in tuberculosis as compared to that in lung cancer, and accumulation of 18F-Alfatide II varied even in lesions of the same patient. The negative results of sarcoidosis patients led to the speculation that αVβ3 was not expressed in those lesions.


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## Body

## 1. Introduction

Lung cancer is one of the largest malignant tumors with fast-growing morbidity and mortality.18F-FDG PET/CT has been verified as a crucial tool for detecting, identifying, and staging lung cancer. However, the specificity of 18F-FDG PET/CT in lung cancer is controversial as some benign lesions such as tuberculosis and sarcoidosis also show a high accumulation of 18F-FDG. Thus, a new tracer with higher specificity in differentiating lung cancer and inflammation is essential.The expression of integrinαVβ3 on the surface of cancer cells and neovascularization endothelial cells is upregulated in cancer, inflammation, and wound [1]. 18F-Alfatide II is an annular dimer RGD (Arg-Gly-Asp) tracer targeting integrin αVβ3. Previous studies reported that the uptake of Alfatide in lung cancer and tuberculosis lesions is markedly different [2, 3]. Thus, additional clinical data is needed to illustrate whether this new tracer is beneficial for the differentiation of tuberculosis from lung cancer.The present study investigated the differential diagnostic value of18F-Alfatide II PET/CT between tuberculosis and lung cancer patients. Also, the angiogenesis in sarcoidosis and chronic inflammations was investigated.


## 2. Materials and Methods

### 2.1. Radiopharmaceutical Preparation

The kit was provided by Jiangsu Institute of Nuclear Medicine. Synthesis of18F-Alfatide II has been described previously [4].


### 2.2. Patients

The local institutional review board approved the18F-Alfatide II PET/CT compliment protocol. Written informed consent was obtained from each patient. The cohort consisted of 9 patients [5 men and 4 women; aged 25–71 (55 ± 16) years] with suspected inflammations and 11 patients [10 men, 1 women; aged 48–78 (66 ± 9) years] with suspected lung cancer.


### 2.3. PET/CT Acquisition and Image Analysis

18F-FDG and 18F-Alfatide II PET/CT were performed at an interval of 2 weeks. Patients were required to fast at least 6 h before 18F-FDG (5.55 MBq/kg) intravenous injection. The acquisitions were conducted at 60 min after the injection. The patients were placed in a supine position on the scanner bed. Imaging data were acquired from the skull to the thigh, using PET/CT scanner, at 1.5 min/bed position. Low-dose CT was performed for attenuation correction and lesion localization. 18F-Alfatide II PET/CT was performed on the next day without any specific preparation before the examination. 18F-Alfatide II (248.27 ± 45.14 MBq) was injected intravenously in all patients. The acquisition procedure and parameters were identical to that as 18F-FDG PET/CT. Regions of interest (ROIs) were drawn manually on the site of lesions based on the corresponding CT images.PET/CT Scanner was from Siemens (Biograph True Point PET/CT).Visual analysis was used to evaluate the preliminary accumulation of18F-Alfatide II and 18F-FDG in tuberculosis and lung cancer. Maximum standard uptake value (SUVmax), mean standard uptake value (SUVmean), and lesion/muscle (L/M) ratio recorded the uptake of the lesions in this study. The uptake of the right hip muscle was selected as a reference for lesions. All lesions were divided into different regions of head-neck, chest, abdomen, and pelvis. The largest lesion of each region was chosen to measure the uncountable lesions.Two physicians evaluated the images independently, and the discrepancies were resolved by consultation.


### 2.4. Pathological Analysis and Follow-Up

Inflammation Group. Number 1 patient was confirmed as thoracic tuberculosis by biopsy. Number 2–4 patients, receiving PPD test, T-SPOT test, and antituberculosis treatment, were followed up for 16, 17, and 33 months, respectively; they were confirmed as lung tuberculosis, lymph node tuberculosis, and lung tuberculosis mixed with tuberculous pleurisy. Number 5 patient was confirmed as sarcoidosis by bronchoscope puncture biopsy. Number 6-7 patients, receiving no treatment and followed up for 17 and 28 months, respectively, were diagnosed as sarcoidosis. Number 8 patient was shown to have chronic inflammation accompanied by fibrosis as assessed by percutaneous lung biopsy. Number 9 patient was diagnosed with inflammation caused by common infection after 20 months’ follow-up based on CT (Table 1).Table 1
Patient demographics (inflammation group).
Patient number Age (years) Sex Histology 1 69 M Thoracic tuberculosis 2 71 M Lung tuberculosis 3 25 F Lymph node tuberculosis 4 63 M Lung tuberculosis + tuberculous pleurisy 5 66 F Sarcoidosis 6 56 F Sarcoidosis 7 32 F Sarcoidosis 8 67 M Chronic-inflammation with fibrosis 9 66 M Common inflammationMalignancy Group. Eight patients received surgery. One patient did not undergo surgery since the pulmonary trunk was invaded by cancer, and two patients were not recommended surgery as distant metastasis detected by PET/CT and MRI. All patients were confirmed by pathology; one distant metastasis patient received bronchoscopy biopsy, while the other underwent clavicle lymph node excision (Table 2).Table 2
Patient demographics (malignancy group).
Patient number Age (years) Sex Histology 1 65 M Squamous cell carcinoma (SCC) 2 75 M Adenocarcinoma 3 78 M Squamous cell carcinoma (SCC) 4 72 M Squamous cell carcinoma (SCC) 5 58 M Squamous cell carcinoma (SCC) 6 57 M Squamous cell carcinoma (SCC) 7 67 M Squamous cell carcinoma (SCC) 8 48 M Squamous cell carcinoma (SCC) 9 57 F Adenocarcinoma 10 75 M Adenocarcinoma 11 74 M Adenocarcinoma


### 2.5. Statistical Analysis

Mean ± standard deviation (m ± SD) was used to express all quantitative data. Differences in SUVmax/SUVmean between patients with different diseases were compared and assessed byt-test or Mann–Whitney U test. All statistical analyses were carried out using SAS 9.2. P < 0.05 indicated statistical significance.


## 2.1. Radiopharmaceutical Preparation

The kit was provided by Jiangsu Institute of Nuclear Medicine. Synthesis of18F-Alfatide II has been described previously [4].


## 2.2. Patients

The local institutional review board approved the18F-Alfatide II PET/CT compliment protocol. Written informed consent was obtained from each patient. The cohort consisted of 9 patients [5 men and 4 women; aged 25–71 (55 ± 16) years] with suspected inflammations and 11 patients [10 men, 1 women; aged 48–78 (66 ± 9) years] with suspected lung cancer.


## 2.3. PET/CT Acquisition and Image Analysis

18F-FDG and 18F-Alfatide II PET/CT were performed at an interval of 2 weeks. Patients were required to fast at least 6 h before 18F-FDG (5.55 MBq/kg) intravenous injection. The acquisitions were conducted at 60 min after the injection. The patients were placed in a supine position on the scanner bed. Imaging data were acquired from the skull to the thigh, using PET/CT scanner, at 1.5 min/bed position. Low-dose CT was performed for attenuation correction and lesion localization. 18F-Alfatide II PET/CT was performed on the next day without any specific preparation before the examination. 18F-Alfatide II (248.27 ± 45.14 MBq) was injected intravenously in all patients. The acquisition procedure and parameters were identical to that as 18F-FDG PET/CT. Regions of interest (ROIs) were drawn manually on the site of lesions based on the corresponding CT images.PET/CT Scanner was from Siemens (Biograph True Point PET/CT).Visual analysis was used to evaluate the preliminary accumulation of18F-Alfatide II and 18F-FDG in tuberculosis and lung cancer. Maximum standard uptake value (SUVmax), mean standard uptake value (SUVmean), and lesion/muscle (L/M) ratio recorded the uptake of the lesions in this study. The uptake of the right hip muscle was selected as a reference for lesions. All lesions were divided into different regions of head-neck, chest, abdomen, and pelvis. The largest lesion of each region was chosen to measure the uncountable lesions.Two physicians evaluated the images independently, and the discrepancies were resolved by consultation.


## 2.4. Pathological Analysis and Follow-Up

Inflammation Group. Number 1 patient was confirmed as thoracic tuberculosis by biopsy. Number 2–4 patients, receiving PPD test, T-SPOT test, and antituberculosis treatment, were followed up for 16, 17, and 33 months, respectively; they were confirmed as lung tuberculosis, lymph node tuberculosis, and lung tuberculosis mixed with tuberculous pleurisy. Number 5 patient was confirmed as sarcoidosis by bronchoscope puncture biopsy. Number 6-7 patients, receiving no treatment and followed up for 17 and 28 months, respectively, were diagnosed as sarcoidosis. Number 8 patient was shown to have chronic inflammation accompanied by fibrosis as assessed by percutaneous lung biopsy. Number 9 patient was diagnosed with inflammation caused by common infection after 20 months’ follow-up based on CT (Table 1).Table 1
Patient demographics (inflammation group).
Patient number Age (years) Sex Histology 1 69 M Thoracic tuberculosis 2 71 M Lung tuberculosis 3 25 F Lymph node tuberculosis 4 63 M Lung tuberculosis + tuberculous pleurisy 5 66 F Sarcoidosis 6 56 F Sarcoidosis 7 32 F Sarcoidosis 8 67 M Chronic-inflammation with fibrosis 9 66 M Common inflammationMalignancy Group. Eight patients received surgery. One patient did not undergo surgery since the pulmonary trunk was invaded by cancer, and two patients were not recommended surgery as distant metastasis detected by PET/CT and MRI. All patients were confirmed by pathology; one distant metastasis patient received bronchoscopy biopsy, while the other underwent clavicle lymph node excision (Table 2).Table 2
Patient demographics (malignancy group).
Patient number Age (years) Sex Histology 1 65 M Squamous cell carcinoma (SCC) 2 75 M Adenocarcinoma 3 78 M Squamous cell carcinoma (SCC) 4 72 M Squamous cell carcinoma (SCC) 5 58 M Squamous cell carcinoma (SCC) 6 57 M Squamous cell carcinoma (SCC) 7 67 M Squamous cell carcinoma (SCC) 8 48 M Squamous cell carcinoma (SCC) 9 57 F Adenocarcinoma 10 75 M Adenocarcinoma 11 74 M Adenocarcinoma


## 2.5. Statistical Analysis

Mean ± standard deviation (m ± SD) was used to express all quantitative data. Differences in SUVmax/SUVmean between patients with different diseases were compared and assessed byt-test or Mann–Whitney U test. All statistical analyses were carried out using SAS 9.2. P < 0.05 indicated statistical significance.


## 3. Results

### 3.1. Safety

Patients did not report any subjective effects following the injected dose of18F-Alfatide II. No adverse events were noted during the examination of 18F-Alfatide II PET/CT or follow-up (at least 6 months).


### 3.2. Visual Analysis Results

The accumulation of18F-Alfatide II in tuberculosis was much lower than that of 18F-FDG (Figures 1 and 2), while no accumulation was observed in sarcoidosis lesions (Figure 3). Additionally, 2 chronic inflammations showed a high accumulation of 18F-Alfatide II.Figure 1
F   18-FDG (a) and F18-Alfatide II (b) PET/CT images of a thoracic tuberculosis patient. T2, T3, and T4 showed intense F18-FDG uptake and mild F18-Alfatide II uptake. The white arrows indicate tuberculosis lesions in T4.
(a) (b)Figure 2
F   18-FDG (a) and F18-Alfatide II (b) PET/CT images of a lymph node tuberculosis patient. Lymph nodes tuberculosis lesions showed intense F18-FDG uptake and no F18-Alfatide II uptake. The white arrows indicate tuberculosis lesions in porta hepatis lymph nodes.
(a) (b)Figure 3
F   18-FDG (a) and F18-Alfatide II (b) PET/CT images of a sarcoidosis patient. All lesions showed intense F18-FDG uptake and no F18-Alfatide II uptake. The white arrows indicate sarcoidosis lesions in mediastinum and hilus pulmonis.
(a) (b)All lung cancer patients in this study showed a high accumulation of18F-Alfatide II, including brain and bone metastasis. The results were similar to that in the previous reports [5].


### 3.3. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Tuberculosis

The SUVmax of tuberculosis was calculated as 7.53 ± 2.88 and 2.63 ± 1.34 for18F-FDG and 18F-Alfatide II, respectively. The SUVmean of tuberculosis was 4.58 ± 1.73 and 1.86 ± 1.0, respectively.


### 3.4. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Chronic Inflammation

The SUVmax of two patients with chronic inflammation was 10.80 and 1.62 for18F-FDG and 9.13 and 5.75 for 18F-Alfatide II, respectively. The SUVmean was 5.33, 0.99 and 5.12, 2.65, respectively.


### 3.5. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Sarcoidosis

The SUVmax of18F-FDG and 18F-Alfatide II in sarcoidosis was calculated as 8.82 ± 5.17 and 1.77 ± 0.69, respectively, while SUVmean was 5.34 ± 3.08 and 1.28 ± 0.63, respectively.


### 3.6. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Lung Cancer

The SUVmax of18F-FDG and 18F-Alfatide II in lung cancer was 12.04 ± 4.67 and 4.08 ± 1.51, respectively, while SUVmean was 4.55 ± 1.98 and 1.99 ± 0.81, respectively.


### 3.7. Difference between the SUVmax of Malignant Lesions and Tuberculosis Lesions

The lesion-to-lesion analysis showed that the SUVmax of18F-Alfatide II in malignant lesions was 4.08 ± 1.51, which was significantly higher than that in tuberculosis (2.63 ± 1.34, P = 0.0078).


### 3.8. Difference between the SUVmean of Malignant Lesions and Tuberculosis Lesions

The SUVmean of18F-Alfatide II in malignant lesions was 1.99 ± 0.81 without any statistically significant difference from that of tuberculosis (1.86 ± 1.0, P = 0.3820).


### 3.9. Preliminary Diagnostic Value of18F-FDG and 18F-Alfatide II PET/CT in Inflammations (Active TB, Chronic Inflammation, and Sarcoidosis) and Lung Cancer

See Table3.Table 3
SUVmax and SUVmean of lesions.
18F-FDG 18F-Alfatide II 18F-FDG 18F-Alfatide II SUVmax SUVmax SUVmean SUVmean Tuberculosis 7.53 ± 2.88 2.63 ± 1.34 4.58 ± 1.73 1.86 ± 1.0 Lung cancer 12.04 ± 4.67 4.08 ± 1.51 4.55 ± 1.98 1.99 ± 0.81 Sarcoidosis 8.82 ± 5.17 1.77 ± 0.69 5.34 ± 3.08 1.28 ± 0.63 Chronic inflammation 1 10.80 9.13 5.33 5.12 Chronic inflammation 2 1.62 5.75 0.99 2.65


## 3.1. Safety

Patients did not report any subjective effects following the injected dose of18F-Alfatide II. No adverse events were noted during the examination of 18F-Alfatide II PET/CT or follow-up (at least 6 months).


## 3.2. Visual Analysis Results

The accumulation of18F-Alfatide II in tuberculosis was much lower than that of 18F-FDG (Figures 1 and 2), while no accumulation was observed in sarcoidosis lesions (Figure 3). Additionally, 2 chronic inflammations showed a high accumulation of 18F-Alfatide II.Figure 1
F   18-FDG (a) and F18-Alfatide II (b) PET/CT images of a thoracic tuberculosis patient. T2, T3, and T4 showed intense F18-FDG uptake and mild F18-Alfatide II uptake. The white arrows indicate tuberculosis lesions in T4.
(a) (b)Figure 2
F   18-FDG (a) and F18-Alfatide II (b) PET/CT images of a lymph node tuberculosis patient. Lymph nodes tuberculosis lesions showed intense F18-FDG uptake and no F18-Alfatide II uptake. The white arrows indicate tuberculosis lesions in porta hepatis lymph nodes.
(a) (b)Figure 3
F   18-FDG (a) and F18-Alfatide II (b) PET/CT images of a sarcoidosis patient. All lesions showed intense F18-FDG uptake and no F18-Alfatide II uptake. The white arrows indicate sarcoidosis lesions in mediastinum and hilus pulmonis.
(a) (b)All lung cancer patients in this study showed a high accumulation of18F-Alfatide II, including brain and bone metastasis. The results were similar to that in the previous reports [5].


## 3.3. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Tuberculosis

The SUVmax of tuberculosis was calculated as 7.53 ± 2.88 and 2.63 ± 1.34 for18F-FDG and 18F-Alfatide II, respectively. The SUVmean of tuberculosis was 4.58 ± 1.73 and 1.86 ± 1.0, respectively.


## 3.4. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Chronic Inflammation

The SUVmax of two patients with chronic inflammation was 10.80 and 1.62 for18F-FDG and 9.13 and 5.75 for 18F-Alfatide II, respectively. The SUVmean was 5.33, 0.99 and 5.12, 2.65, respectively.


## 3.5. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Sarcoidosis

The SUVmax of18F-FDG and 18F-Alfatide II in sarcoidosis was calculated as 8.82 ± 5.17 and 1.77 ± 0.69, respectively, while SUVmean was 5.34 ± 3.08 and 1.28 ± 0.63, respectively.


## 3.6. Preliminary Diagnostic Value of18F-FDG PET/CT and 18F-Alfatide II PET/CT in Lung Cancer

The SUVmax of18F-FDG and 18F-Alfatide II in lung cancer was 12.04 ± 4.67 and 4.08 ± 1.51, respectively, while SUVmean was 4.55 ± 1.98 and 1.99 ± 0.81, respectively.


## 3.7. Difference between the SUVmax of Malignant Lesions and Tuberculosis Lesions

The lesion-to-lesion analysis showed that the SUVmax of18F-Alfatide II in malignant lesions was 4.08 ± 1.51, which was significantly higher than that in tuberculosis (2.63 ± 1.34, P = 0.0078).


## 3.8. Difference between the SUVmean of Malignant Lesions and Tuberculosis Lesions

The SUVmean of18F-Alfatide II in malignant lesions was 1.99 ± 0.81 without any statistically significant difference from that of tuberculosis (1.86 ± 1.0, P = 0.3820).


## 3.9. Preliminary Diagnostic Value of18F-FDG and 18F-Alfatide II PET/CT in Inflammations (Active TB, Chronic Inflammation, and Sarcoidosis) and Lung Cancer

See Table3.Table 3
SUVmax and SUVmean of lesions.
18F-FDG 18F-Alfatide II 18F-FDG 18F-Alfatide II SUVmax SUVmax SUVmean SUVmean Tuberculosis 7.53 ± 2.88 2.63 ± 1.34 4.58 ± 1.73 1.86 ± 1.0 Lung cancer 12.04 ± 4.67 4.08 ± 1.51 4.55 ± 1.98 1.99 ± 0.81 Sarcoidosis 8.82 ± 5.17 1.77 ± 0.69 5.34 ± 3.08 1.28 ± 0.63 Chronic inflammation 1 10.80 9.13 5.33 5.12 Chronic inflammation 2 1.62 5.75 0.99 2.65


## 4. Discussion

IntegrinαVβ3 is overexpressed not only in various tumor cells and tumor neovasculature [6] but also in chronic inflammatory diseases, such as inflammatory bowel disease (IBD) and rheumatoid arthritis (RA) [7, 8]. Previous studies reported that angiogenesis and chronic inflammation are interrelated [7, 9]. Jackson et al. [10] suggested that several resident cells (fibroblasts, monocytes-macrophages, neutrophils, and lymphocytes) can promote angiogenesis when the microenvironment becomes hypoxic or inflammatory, thereby facilitating the migration of inflammatory cells to inflammatory sites and the supply of nutrients and oxygen to the proliferating tissue. The frequent dual functionality of angiogenic factors such as αVβ3 and VEGF reflects the close relationship between angiogenesis and inflammation [11]. Cao et al. [12] demonstrated specific uptake of αVβ3 in the chronic inflammation of mouse ear using 64Cu-DOTA-RGD tetramer PET imaging.Chin et al. [13] carried out a PET imaging study with 18F-FPP(RGD)2 in a healthy woman volunteer, and no unusual or adverse patient symptoms were found on the day of imaging as well as during follow-up. Wan et al. [2] did not record any adverse events associated with 18F-Alfatide in the first subject during the study, in which nine lung cancer patients were investigated. No adverse events occurred in all patients during or after the 18F-Alfatide II PET/CT imaging in the current study. All these investigations revealed that using RGD tracers labeled by 18F is safe for patients.Tuberculosis is a major global health problem with an estimated 8.6 million new cases worldwide in 2012 [14]. The tuberculous granuloma is an organized collection of differentiated macrophages surrounded by T-lymphocytes, B-lymphocytes, dendritic cells, fibroblasts, and extracellular matrix components [15]. Some studies demonstrated αVβ1 expression in lung granulomas and lymph nodes of sarcoid patients [16]. Rojas et al. [17] found that the mycobacterial glycolipid phosphatidylinositol mannoside interacts directly with αVβ1 integrin VLA-5 on CD4+ T-lymphocytes, resulting in fibronectin binding and T-cell migration. Several studies have reported increased levels of VEGF in granulomatous disease, such as pulmonary tuberculosis [18–21] and Crohn’s disease [22]. Hur et al. [23] reported that median concentration of serum VEGF-A was significantly higher in tuberculosis patients than that in the latent tuberculosis infection and control groups. Four patients with active tuberculosis showed varying degree of accumulation of 18F-Alfatide II in this study, including negative results, and even the positive lesions showed a low accumulation of 18F-Alfatide II than that of 18F-FDG. Kang et al. [3] reported that tuberculosis granuloma and the surrounding vasculature epithelium showed baseline αVβ3 expression as assessed by immunohistochemistry. The diversity in the current study revealed different expression of αVβ3 in all tuberculosis lesions.All lung cancer lesions and the metastases in the brain and bone showed an increased RGD uptake. A significant difference was noted in SUVmax between the lung cancer and tuberculosis groups, which indicated that RGD PET/CT might differentiate lung cancer from tuberculosis.Sarcoidosis is an immunological, granulomatous disorder affecting multiple systems. The presence of noncaseating granulomas in involved organs is a pathological feature [24]. The common sites of the disease are lung, mediastinum, and hilus pulmonis lymph node [25, 26]. The precise pathogenesis is yet unknown, which might include various factors: environmental, occupational exposure, the presence of infectious agents, and genetic susceptibility [27–29]. Various studies suggested that angiogenic factors contribute to the pathogenesis of sarcoidosis [30, 31]. Agostini et al. [32] and Antoniou et al. [31] indicated the presence of angiogenesis in the pathogenesis of granulomatous and pulmonary fibrosis. Tzouvelekis et al. [33] revealed an abundant expression of VEGF and ING4 within the granulomatous tissue, localized in the epithelioid and giant cells. Three sarcoidosis patients, in this study, showed negative results in 18F-Alfatide II PET/CT, thereby indicating the lack of αVβ3 expression in sarcoidosis. Kambouchner et al. [34] proposed the presence of an avascular microenvironment within sarcoid lesions. Tzouvelekis et al. [33] speculated that abundant expression of VEGF might be implicated in the inflammatory than the angiogenic cascade of sarcoidosis. Murdoch et al. supported this speculation with respect to the pleiotropic properties of VEGF in promoting the Th1-dependent immunity via facilitation of monocyte recruitment and T-cell migration to sites of ongoing inflammation [35]. The results from the current study were in agreement with the theory by Kambouchner et al. and Tzouvelekis et al.Furthermore, the present study comprised 2 common infection patients: one patient showed high accumulation of18F-Alfatide II as well as 18F-FDG, while the other showed a high accumulation of 18F-Alfatide II compared to 18F-FDG. Winter et al. [36] speculated that integrin αVβ3 was a potential marker of inflammation and angiogenesis in atherosclerotic lesions. Srivatsa et al. and Hansson both observed persistently high levels of αVβ3 expression between 7 and 21 days following injury in the neointima, media, and adventitia [37, 38]. Other studies demonstrated the expression of integrin αVβ3 on activated macrophages by different methods [39–41]. When acute inflammation transforms into subacute and chronic inflammation, macrophages are gradually increased in number in lesions than the neutrophils. Thus, the high accumulation of 18F-Alfatide II in the 2 patients in this study indicated the chronic inflammatory stage, which was confirmed by fibrosis tested by percutaneous lung biopsy in one lesion. Storgard et al. [42] reported that the treatment with cyclic RGD peptide c(RGDfV), an integrin αVβ3 antagonist, significantly inhibited the disease progression in an experimental RA model. Taken together, 18F-Alfatide II PET/CT may not only detect chronic inflammation but also allow the evaluation of angiogenesis and neovascularization during chronic inflammation and guide the selection of patients for antiangiogenesis therapy.Nevertheless, the present study has some deficiencies. (1) Early experimental design was not perfect; for example, we did not recruit lymphoma patients to compare with sarcoidosis in18F-Alfatide II PET/CT. (2) The number of patients was small. (3) Further investigations are essential.


## 5. Conclusion

The accumulation of lung cancer and tuberculosis exhibits distinct difference, which might be valuable in differentiating the two diseases. Three sarcoidoses showed negative results, and thus, we speculated the lack ofαVβ3 expression within sarcoidosis. 18F-Alfatide II might be valuable in the evaluation of angiogenesis and neovascularization during chronic inflammation, which could guide the selection of patients for antiangiogenesis therapy and evaluate the clinical effect of the treatment.



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