# Thromboelastogram-Guided Transfusion Therapy Reduces Blood-Component Transfusion and Improves Coagulation Function during Orthopedic Surgery

Authors: Yan Zhang; Yue Song; Yixin Zhang; Lu Yu; Kai Zhang
Journal: Journal of Nanomaterials
 (2021)

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## Abstract

Massive bleeding is a serious medical complication arising from trauma, surgery, and invasive procedures. In this case, timely and effective hemostasis must be applied to patients. The aim of this study is to compare transfusion therapies guided by thromboelastogram (TEG) and conventional coagulation tests (CCTs) during orthopedic surgery, focusing on blood-component transfusion and coagulation function of patients. The patients who underwent orthopedic surgery in our hospital from May 2019 to November 2020 were retrospectively analyzed. According to different transfusion-guiding strategies, the patients were assigned into the CCT group containing 214 patients and the TEG group containing 266 patients. The TEG group used fewer volumes of blood products including red blood cell (RBC) suspension, fresh-frozen plasma, cryoprecipitate, and apheresis platelets than the CCT group (P<0.05). After orthopedic surgery, the hemoglobin (Hb) and RBC counts were decreased, but the white blood cell (WBC) counts were increased in all patients receiving whether transfusion therapy guided by TEG or CCTs. Importantly, the TEG group exhibited fewer WBC counts concomitant with higher Hb and more RBC counts than the CCT group (P<0.05). There was no significant difference on the platelet (PLT) counts between the two groups before and after orthopedic surgery (P>0.05). Significant declines on thrombin time (TT), partial activated thromboplastin time (PATT), prothrombin time (PT), and d-dimer (D-D), along with an increase on fibrinogen (FIB) were observed in two groups after surgery. The TEG group showed reduced TT, PATT, PT, D-D, and elevated FIB compared to the CCT group (P<0.05). Posttransfusion, the K value (time to reach a certain clot strength) and R value (coagulation reaction time) were decreased, the angle (α) value (clot formation rate), MA value (maximum amplitude), and CI (coagulation index) were increased in the TEG group (P<0.05). When the liver function was assessed, it was found that the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total serum bilirubin (TBIL) were increased significantly, and albumin (ALB) was decreased between the two groups after surgery, but the TEG group with lower levels of ALT, AST, and TBIL and a higher level of ALB than the CCT group (P<0.05). With regard to the renal function, two groups had increased levels of serum creatinine (Scr) and blood urea nitrogen (BUN) with a declined uric acid (UA) level after surgery; however, the patients in the TEG group had lower levels of Scr and BUN and a higher level of UA compared to the CCT group (P<0.05). In view of above data, TEG-guided transfusion therapy could reduce use of blood products, optimize blood components, and improve coagulation function for patients undergoing orthopedic surgery. TEG-guided transfusion therapy may prevent liver and renal dysfunction after orthopedic surgery.


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## Body

## 1. Introduction

Massive blood lose is a risk for mortality and morbidity during orthopedic surgeries [1], including total hip replacement, total knee replacement [2], and spine surgery [3]. Massive bleeding is a serious and life-threatening manifestation, which can cause a variety of complications, including coagulation abnormalities, immunosuppression, infection, hypothermia, and organ damage related with massive transfusion of blood products [4, 5]. Blood transfusion involves red blood cell (RBC) suspension, fresh-frozen plasma, cryoprecipitate, and apheresis platelets, which is critical to the patients with massive bleeding [6]. Conventional coagulation tests (CCTs), such as thrombin time (TT), partial activated thromboplastin time (PATT), prothrombin time (PT), and fibrinogen (FIB), are related to the dose and timing of transfusion [7, 8]. Shortage of blood products makes accurate judgment for time of blood transfusion and requirements of blood components urgent. However, the present massive blood transfusion protocol is fixed infusion of RBC, fresh frozen plasma (FFP), and platelets (PLTs) at a ratio of 1 : 1 : 1, likely leading to a huge waste. Thromboelastogram (TEG) has been advanced by Dr. Hartert in 1948 [9], which comprehensively analyzes the coagulation markers and represents the coagulation activity from coagulation cascade to fibrinolysis, which reflects the whole hemostasis process [10]. Studies have shown that TEG has been played a crucial role in the guidance of transfusion in cardiac surgeries for decades [11, 12]. Present researchers have attempted to establish a transfusion strategy guided by TEG during the perioperative period. However, due to variations in sample size and individual differences, different conclusions have been reported. There is limited evidence supporting its application in orthopedic surgery, and thus this study attempts to compare transfusion therapies guided by TEG and CCTs during orthopedic surgery, with a specific focus on blood-component transfusion and coagulation function of patients.


## 2. Methods

### 2.1. Patient Enrollment

From May 2019 to November 2020, patients who had undergone orthopedic surgery in our hospital were retrospectively studied and then divided into a TEG group (n=266) and a CCT group (n=214) in accordance with transfusion therapies guided by TEG or CCTs. All they belonged to class I-II according to the American Society of Aneshesiologists (ASA) Physical Status Classification System. Those patients were excluded due to use of anticoagulants in half a year, cognitive impairment, hematological diseases, coagulation dysfunction, hepatic and renal insufficiency, RH blood group, chronic alcoholics, pregnant women, and absence of complete clinical data. Patients in the TEG group consisted of 201 males and 66 females, aged from 13-85 years and with an average of age (48.56±21) years and underwent orthopedic surgery for lumbar spinal stenosis (n=55), cervical spondylosis (n=50), total knee replacement (n=55), fracture (n=100), and lumbar disc herniation (n=6). Patients in the CCT group comprised of 162 males and 52 females, aged from 13-84 years and with an average of age (48.54±29) years and underwent orthopedic surgery for lumbar spinal stenosis (n=44), cervical spondylosis (n=40), total knee replacement (n=43), fracture (n=80), and lumbar disc herniation (n=7). The baseline information between the two groups was not significant (P>0.05).


### 2.2. Transfusion Therapy Guided by TEG or CCTs

The automatic coagulation analyzer SYSMEX CA 7000 (TOA Medical Electronics Co., Kobe, Japan) and the hematology analyzer (XN-3000, TOA Medical Electronics Co.) were used to examine PT (normal range [s]: 11.0-13.0), APTT (normal range [s]: 23.00-37.00), TT (normal range [s]: 12.0-19.0), and FIB (normal range [g/L]: 2.0-4.0) before and after transfusion. When the hemoglobin (Hb) was lower than 70 g/L and hematocrit (HTC) was less than 25%, RBC suspension (2 U) was transfused. If the PLT (normal range [×109/L]: 100-300) was lower than 50×109/L, apheresis platelet (10 U) was infused.The TEG analyzer (TEG5000, Haemoscope, USA) was used to analyzeR time (coagulation reaction time), K time (time to reach a certain clot strength), α value (clot formation rate), MA value (maximum amplitude), and CI (coagulation index). The normal R time ranges from 5 min to 8 min, reflecting the function of coagulation factors. Prolonged R time indicates deficiency of coagulation factors, and shortened R time indicates hypercoagulability. The normal K time ranges from 1 min to 3 min, depending on the levels of FIB, and anticoagulants could delay the K time. The α value ranges from 53° to 72°, reflecting the rate of clot formation during which FIB and PLTs are both involved. Once severe hypocoagulability occurs, clot amplitude fails to reach 20 mm, and the K time is undetectable. In this situation, the α value is more significant. A greater angle reflects faster clot formation. Prolonged K time with low angle indicates risk of hypocoagulability and blooding. Shortened K time with high angle indicates risk of hypercoagulability and clot. The normal MA value ranges from 54 mm to 72 mm, which is affected by FIB (20%) and PLT (80%), suggesting the accumulation of PLT. A smaller MA value suggests blooding, dilute blood, reduced numbers of PLTs, depletion, or deficiency of coagulation factors. A greater MA value suggests arterial and venous thrombosis and hypercoagulability. The normal CI ranges from -3 to 3 and equals −0.6516r−0.3772K+0.1224MA+−7.7922. CI>3 suggests hypercoagulability, and CI<−3 suggests hypocoagulability. When the R value was more than 8 min, fresh-frozen plasma (15 mL/kg) was infused. When the MA value was less than 50 mm, the apheresis platelet (10 U) was infused. The cryoprecipitate (13 U) was infused if the angle value was more than 72°. The remaining blood products were transfused according to patients’ condition and clinical experience. The usage of RBC suspension, fresh-frozen plasma, cryoprecipitate, and apheresis platelet was recorded.


### 2.3. Measurements of Liver and Renal Function

Fasting venous blood was collected into EDTA-contained tubes which were then centrifuged to extract the plasma. Blood sample collection was performed pretransfusion and posttransfusion. According to the instructions provided by commercial available kits, aminotransferase (ALT), aspartate aminotransferase (AST), total serum bilirubin (TBIL), albumin (ALB), serum creatinine (Scr), blood urea nitrogen (BUN), and uric acid (UA) were examined by the enzyme linked immunosorbent assay pretransfusion and posttransfusion.


### 2.4. Data Analysis

SPSS 20.0 software was used to process the data. The counting data was represented by percentage and analyzed by the chi-square test. The measurement data was represented bymean±standarddeviation and analyzed by the t-test. A level of P<0.05 suggests a statistically significant difference.


## 2.1. Patient Enrollment

From May 2019 to November 2020, patients who had undergone orthopedic surgery in our hospital were retrospectively studied and then divided into a TEG group (n=266) and a CCT group (n=214) in accordance with transfusion therapies guided by TEG or CCTs. All they belonged to class I-II according to the American Society of Aneshesiologists (ASA) Physical Status Classification System. Those patients were excluded due to use of anticoagulants in half a year, cognitive impairment, hematological diseases, coagulation dysfunction, hepatic and renal insufficiency, RH blood group, chronic alcoholics, pregnant women, and absence of complete clinical data. Patients in the TEG group consisted of 201 males and 66 females, aged from 13-85 years and with an average of age (48.56±21) years and underwent orthopedic surgery for lumbar spinal stenosis (n=55), cervical spondylosis (n=50), total knee replacement (n=55), fracture (n=100), and lumbar disc herniation (n=6). Patients in the CCT group comprised of 162 males and 52 females, aged from 13-84 years and with an average of age (48.54±29) years and underwent orthopedic surgery for lumbar spinal stenosis (n=44), cervical spondylosis (n=40), total knee replacement (n=43), fracture (n=80), and lumbar disc herniation (n=7). The baseline information between the two groups was not significant (P>0.05).


## 2.2. Transfusion Therapy Guided by TEG or CCTs

The automatic coagulation analyzer SYSMEX CA 7000 (TOA Medical Electronics Co., Kobe, Japan) and the hematology analyzer (XN-3000, TOA Medical Electronics Co.) were used to examine PT (normal range [s]: 11.0-13.0), APTT (normal range [s]: 23.00-37.00), TT (normal range [s]: 12.0-19.0), and FIB (normal range [g/L]: 2.0-4.0) before and after transfusion. When the hemoglobin (Hb) was lower than 70 g/L and hematocrit (HTC) was less than 25%, RBC suspension (2 U) was transfused. If the PLT (normal range [×109/L]: 100-300) was lower than 50×109/L, apheresis platelet (10 U) was infused.The TEG analyzer (TEG5000, Haemoscope, USA) was used to analyzeR time (coagulation reaction time), K time (time to reach a certain clot strength), α value (clot formation rate), MA value (maximum amplitude), and CI (coagulation index). The normal R time ranges from 5 min to 8 min, reflecting the function of coagulation factors. Prolonged R time indicates deficiency of coagulation factors, and shortened R time indicates hypercoagulability. The normal K time ranges from 1 min to 3 min, depending on the levels of FIB, and anticoagulants could delay the K time. The α value ranges from 53° to 72°, reflecting the rate of clot formation during which FIB and PLTs are both involved. Once severe hypocoagulability occurs, clot amplitude fails to reach 20 mm, and the K time is undetectable. In this situation, the α value is more significant. A greater angle reflects faster clot formation. Prolonged K time with low angle indicates risk of hypocoagulability and blooding. Shortened K time with high angle indicates risk of hypercoagulability and clot. The normal MA value ranges from 54 mm to 72 mm, which is affected by FIB (20%) and PLT (80%), suggesting the accumulation of PLT. A smaller MA value suggests blooding, dilute blood, reduced numbers of PLTs, depletion, or deficiency of coagulation factors. A greater MA value suggests arterial and venous thrombosis and hypercoagulability. The normal CI ranges from -3 to 3 and equals −0.6516r−0.3772K+0.1224MA+−7.7922. CI>3 suggests hypercoagulability, and CI<−3 suggests hypocoagulability. When the R value was more than 8 min, fresh-frozen plasma (15 mL/kg) was infused. When the MA value was less than 50 mm, the apheresis platelet (10 U) was infused. The cryoprecipitate (13 U) was infused if the angle value was more than 72°. The remaining blood products were transfused according to patients’ condition and clinical experience. The usage of RBC suspension, fresh-frozen plasma, cryoprecipitate, and apheresis platelet was recorded.


## 2.3. Measurements of Liver and Renal Function

Fasting venous blood was collected into EDTA-contained tubes which were then centrifuged to extract the plasma. Blood sample collection was performed pretransfusion and posttransfusion. According to the instructions provided by commercial available kits, aminotransferase (ALT), aspartate aminotransferase (AST), total serum bilirubin (TBIL), albumin (ALB), serum creatinine (Scr), blood urea nitrogen (BUN), and uric acid (UA) were examined by the enzyme linked immunosorbent assay pretransfusion and posttransfusion.


## 2.4. Data Analysis

SPSS 20.0 software was used to process the data. The counting data was represented by percentage and analyzed by the chi-square test. The measurement data was represented bymean±standarddeviation and analyzed by the t-test. A level of P<0.05 suggests a statistically significant difference.


## 3. Results

### 3.1. Reduced Blood Products by TEG-Guided Transfusion Therapy

The transfusion of RBCs and nonRBC components which involves fresh-frozen plasma, platelet, and cryoprecipitate is critical to hemostasis. The TEG group used fewer volumes of blood products including RBC suspension, fresh-frozen plasma, cryoprecipitate, and apheresis platelets than the CCT group (P<0.05) (P<0.05, Table 1). In addition, results showed that the TEG group had a shorter length of hospital stay than the CCT group (P<0.001, Table 1).Table 1
The usage of blood products between the TEG and CCT groups.
GroupRBC suspension (U)Fresh frozen plasma (mL)Cryoprecipitate (U)Apheresis platelet (10 U)Length of hospital stay (d)TEG (n=266)4.51±0.09a254.36±20.17a16.34±1.25a2.04±0.06a10.35±2.11aCCT (n=214)6.09±0.04423.54±40.2828.64±2.313.42±0.0811.17±2.23The letter of a suggestsP<0.05 compared to the CCT group.


### 3.2. Blood Routine Tests following TEG- or CCT-Guided Transfusion Therapy

Prior to orthopedic surgery, there was no significant difference on the value of Hb, WBC, PLT, and RBC between the TEG group and CCT group (P>0.05). As listed in Table 2, after orthopedic surgery, the Hb and RBC counts were decreased, but the WBC counts were increased in all patients receiving whether transfusion therapy guided by TEG or CCTs. Importantly, the TEG group exhibited fewer WBC counts concomitant with higher Hb and more RBC counts than the CCT group (P<0.05). There was no significant difference on PLT counts between the two groups before and after surgery (P>0.05).Table 2
Blood routine tests following TEG- or CCT-guided transfusion therapy.
GroupTimeHb (g/L)WBC (×109/L)PLT (×109/L)RBC (×1012/L)TEG (n=266)Preoperation126.41±12.418.75±1.22238.41±20.333.97±0.09Postoperation106.31±11.25ab10.08±1.22ab149.32±12.41a3.30±0.06abCCT (n=214)Preoperation125.32±12.448.26±1.34235.26±20.123.98±0.08Postoperation76.56±8.44a14.36±1.15a148.26±12.41a3.11±0.05aThe letter of a suggestsP<0.05 compared to the CCT group. The letter of b suggests P<0.05 compared to preoperation.


### 3.3. Changes of the Coagulation Function following Transfusion Therapy Guided by TEG or CCTs

There was little difference on the PT, APTT, TT, FIB, and D-D between the two groups before surgery (P>0.05). Significant declines on TT, PATT, PT, and D-D, along with an increase on FIB were observed in two groups after surgery. The TEG group showed reduced TT, PATT, PT, and D-D and elevated FIB compared to the CCT group, as revealed in Table 3 (P<0.05).Table 3
Changes of coagulation function following transfusion therapy guided by TEG or CCTs.
GroupTimePT (s)APTT (s)TT (s)FIB (g/L)D-D (ng/mL)TEG (n=266)Preoperation20.31±1.0641.08±4.6426.26±1.315.07±0.05ab814.41±50.33Postoperation10.14±1.22ab29.08±3.22ab15.54±1.26ab2.27±0.09760.26±50.33aCCT (n=214)Preoperation20.32±1.1141.06±4.1126.33±2.175.09±0.05ab814.26±50.29Postoperation13.31±2.18a33.61±4.33a18.64±1.21a3.33±0.04a783.36±50.41aThe letter of a suggestsP <0.05 compared to the CCT group. The letter of b suggests P <0.05 compared to pre-operation.


### 3.4. Changes of Coagulation Indexes on the TEG before and after Transfusion

The following TEG variables are associated with the coagulation function. TheR value indicates the time to initial fibrin formation; the K value suggests the time until specific clot strength of 20 mm obtained; the MA reveals the final strength of the clot; the angle value shows rate of clot formation; the CI is obtained by the calculation of the R, K, α and MA. As shown in Table 4, the K time and R time were significantly shortened, while the values of MA, CI, and angle were increased after transfusion (P<0.05).Table 4
Changes of coagulation indexes on the TEG before and after transfusion.
TimeK time (min)R time (min)MA value (mm)CIAngle (°)Before transfusion4.54±0.037.27±0.0747.64±5.11−2.71±0.0553.85±6.22After transfusion3.67±0.05b5.96±0.05b57.74±5.22b1.46±0.22b62.71±6.13bK time: time to reach a certain clot strength; R time: coagulation reaction time; angle (α): clot formation rate; MA value: maximum amplitude; CIn: coagulation index. The letter of b suggests P<0.05 compared to pretransfusion.


### 3.5. TEG-Guided Transfusion Therapy Prevented Liver Dysfunction

There was slight difference on the levels of ALT, AST, ALB, and TBIL between the TEG and CCT groups before orthopedic surgery (P>0.05). After surgery, increased levels of ALT, AST, and TBIL concomitant with a decreased level of ALB were indicated in the TEG and CCT groups. However, the TEG group showed lower levels of ALT, AST, and TBIL and a higher level of ALB compared to the CCT group (P<0.05, Table 5).Table 5
Changes of liver function indexes between the TEG and CCT groups.
GroupTimeALT (U/L)AST (U/L)ALB (g/mL)TBIL (μmol/L)TEG (n=266)Preoperation32.27±3.1438.41±3.2233.31±3.2518.47±1.26Postoperation41.82±4.33b65.64±7.14b26.68±2.33b20.49±2.33bCCT (n=214)Preoperation32.25±3.1138.21±2.3633.32±3.0918.45±1.17Postoperation47.21±4.19b72.59±5.41b21.21±2.35b23.64±2.17bThe letter of b suggestsP<0.05 compared to preoperation.


### 3.6. TEG-Guided Transfusion Therapy Prevented Renal Dysfunction

Permanent renal dysfunction is regarded as a serious complication occurring following major surgery, possibly resulting in increased morbidity and mortality. Considering that, the renal function of patients undergoing orthopedic surgery was compared between the TEG and CCT groups. There was no significant difference on the levels of BUN, Scr, and UA between the TEG and CCT groups before surgery (P>0.05). As listed in Table 6, there was an increase on the levels of BUN and Scr, with a decline on the level of UA in the two groups after surgery, but the levels of BUN and Scr were lower, and the level of UA was higher in the TEG group than those in the CCT group (P<0.05).Table 6
Changes of renal function indexes between the TEG and CCT groups.
GroupTimeBUN (mmol/L)Scr (μmol/L)UA (μmol/L)TEG (n=266)Preoperation6.13±0.1261.91±6.41363.25±30.17Postoperation7.21±0.11b81.95±6.21b314.41±20.33bCCT (n=214)Preoperation6.13±0.1861.93±6.22362.19±31.33Postoperation7.69±0.12b96.58±6.17b297.26±20.33bThe letter of b suggestsP<0.05 compared to preoperation.


## 3.1. Reduced Blood Products by TEG-Guided Transfusion Therapy

The transfusion of RBCs and nonRBC components which involves fresh-frozen plasma, platelet, and cryoprecipitate is critical to hemostasis. The TEG group used fewer volumes of blood products including RBC suspension, fresh-frozen plasma, cryoprecipitate, and apheresis platelets than the CCT group (P<0.05) (P<0.05, Table 1). In addition, results showed that the TEG group had a shorter length of hospital stay than the CCT group (P<0.001, Table 1).Table 1
The usage of blood products between the TEG and CCT groups.
GroupRBC suspension (U)Fresh frozen plasma (mL)Cryoprecipitate (U)Apheresis platelet (10 U)Length of hospital stay (d)TEG (n=266)4.51±0.09a254.36±20.17a16.34±1.25a2.04±0.06a10.35±2.11aCCT (n=214)6.09±0.04423.54±40.2828.64±2.313.42±0.0811.17±2.23The letter of a suggestsP<0.05 compared to the CCT group.


## 3.2. Blood Routine Tests following TEG- or CCT-Guided Transfusion Therapy

Prior to orthopedic surgery, there was no significant difference on the value of Hb, WBC, PLT, and RBC between the TEG group and CCT group (P>0.05). As listed in Table 2, after orthopedic surgery, the Hb and RBC counts were decreased, but the WBC counts were increased in all patients receiving whether transfusion therapy guided by TEG or CCTs. Importantly, the TEG group exhibited fewer WBC counts concomitant with higher Hb and more RBC counts than the CCT group (P<0.05). There was no significant difference on PLT counts between the two groups before and after surgery (P>0.05).Table 2
Blood routine tests following TEG- or CCT-guided transfusion therapy.
GroupTimeHb (g/L)WBC (×109/L)PLT (×109/L)RBC (×1012/L)TEG (n=266)Preoperation126.41±12.418.75±1.22238.41±20.333.97±0.09Postoperation106.31±11.25ab10.08±1.22ab149.32±12.41a3.30±0.06abCCT (n=214)Preoperation125.32±12.448.26±1.34235.26±20.123.98±0.08Postoperation76.56±8.44a14.36±1.15a148.26±12.41a3.11±0.05aThe letter of a suggestsP<0.05 compared to the CCT group. The letter of b suggests P<0.05 compared to preoperation.


## 3.3. Changes of the Coagulation Function following Transfusion Therapy Guided by TEG or CCTs

There was little difference on the PT, APTT, TT, FIB, and D-D between the two groups before surgery (P>0.05). Significant declines on TT, PATT, PT, and D-D, along with an increase on FIB were observed in two groups after surgery. The TEG group showed reduced TT, PATT, PT, and D-D and elevated FIB compared to the CCT group, as revealed in Table 3 (P<0.05).Table 3
Changes of coagulation function following transfusion therapy guided by TEG or CCTs.
GroupTimePT (s)APTT (s)TT (s)FIB (g/L)D-D (ng/mL)TEG (n=266)Preoperation20.31±1.0641.08±4.6426.26±1.315.07±0.05ab814.41±50.33Postoperation10.14±1.22ab29.08±3.22ab15.54±1.26ab2.27±0.09760.26±50.33aCCT (n=214)Preoperation20.32±1.1141.06±4.1126.33±2.175.09±0.05ab814.26±50.29Postoperation13.31±2.18a33.61±4.33a18.64±1.21a3.33±0.04a783.36±50.41aThe letter of a suggestsP <0.05 compared to the CCT group. The letter of b suggests P <0.05 compared to pre-operation.


## 3.4. Changes of Coagulation Indexes on the TEG before and after Transfusion

The following TEG variables are associated with the coagulation function. TheR value indicates the time to initial fibrin formation; the K value suggests the time until specific clot strength of 20 mm obtained; the MA reveals the final strength of the clot; the angle value shows rate of clot formation; the CI is obtained by the calculation of the R, K, α and MA. As shown in Table 4, the K time and R time were significantly shortened, while the values of MA, CI, and angle were increased after transfusion (P<0.05).Table 4
Changes of coagulation indexes on the TEG before and after transfusion.
TimeK time (min)R time (min)MA value (mm)CIAngle (°)Before transfusion4.54±0.037.27±0.0747.64±5.11−2.71±0.0553.85±6.22After transfusion3.67±0.05b5.96±0.05b57.74±5.22b1.46±0.22b62.71±6.13bK time: time to reach a certain clot strength; R time: coagulation reaction time; angle (α): clot formation rate; MA value: maximum amplitude; CIn: coagulation index. The letter of b suggests P<0.05 compared to pretransfusion.


## 3.5. TEG-Guided Transfusion Therapy Prevented Liver Dysfunction

There was slight difference on the levels of ALT, AST, ALB, and TBIL between the TEG and CCT groups before orthopedic surgery (P>0.05). After surgery, increased levels of ALT, AST, and TBIL concomitant with a decreased level of ALB were indicated in the TEG and CCT groups. However, the TEG group showed lower levels of ALT, AST, and TBIL and a higher level of ALB compared to the CCT group (P<0.05, Table 5).Table 5
Changes of liver function indexes between the TEG and CCT groups.
GroupTimeALT (U/L)AST (U/L)ALB (g/mL)TBIL (μmol/L)TEG (n=266)Preoperation32.27±3.1438.41±3.2233.31±3.2518.47±1.26Postoperation41.82±4.33b65.64±7.14b26.68±2.33b20.49±2.33bCCT (n=214)Preoperation32.25±3.1138.21±2.3633.32±3.0918.45±1.17Postoperation47.21±4.19b72.59±5.41b21.21±2.35b23.64±2.17bThe letter of b suggestsP<0.05 compared to preoperation.


## 3.6. TEG-Guided Transfusion Therapy Prevented Renal Dysfunction

Permanent renal dysfunction is regarded as a serious complication occurring following major surgery, possibly resulting in increased morbidity and mortality. Considering that, the renal function of patients undergoing orthopedic surgery was compared between the TEG and CCT groups. There was no significant difference on the levels of BUN, Scr, and UA between the TEG and CCT groups before surgery (P>0.05). As listed in Table 6, there was an increase on the levels of BUN and Scr, with a decline on the level of UA in the two groups after surgery, but the levels of BUN and Scr were lower, and the level of UA was higher in the TEG group than those in the CCT group (P<0.05).Table 6
Changes of renal function indexes between the TEG and CCT groups.
GroupTimeBUN (mmol/L)Scr (μmol/L)UA (μmol/L)TEG (n=266)Preoperation6.13±0.1261.91±6.41363.25±30.17Postoperation7.21±0.11b81.95±6.21b314.41±20.33bCCT (n=214)Preoperation6.13±0.1861.93±6.22362.19±31.33Postoperation7.69±0.12b96.58±6.17b297.26±20.33bThe letter of b suggestsP<0.05 compared to preoperation.


## 4. Discussion

The substantial blood loss happens in the trauma, obstetrical, and surgical surgery [13]. In various clinical settings, the massive bleeding is highly related to the mortality and morbidity, especially for severe trauma patients [14]. The massive blood loss reveals the challenge to the coagulation system [3]. Blood transfusion is a relatively safe but with potential adverse reactions, which might be fatal [15]. Therefore, it is necessary to evaluate thehemostasis function and correct coagulation disorder in time, and an appropriate transfusion management should be made [16].The TEG as a viscoelastic hemostasis assay which evaluates coagulation and fibrinolysis properties by the use of whole blood samples [17], which measures the K value, R value, the angle value, MA value, and CI, and these coagulation parameters are associated with clot factor, platelet count, FIB function, and thrombus risk [18, 19]. According to the data in this study, we found that the above parameters changed after transfusion, the K value and R value were highly decreased, and the value of MA, CI, and angle was obviously increased, which indicated the increase on clot factors and enhancement on FIB function and platelet. The PT, APTT, TT, and FIB were the basic indexes to judge whether the coagulation function is normal or not [20], and the D-D is related to the fibrinolytic activity [21]. Significant declines on TT, PATT, PT, and D-D, along with an increase on FIB were observed in two groups after surgery. The TEG group showed reduced TT, PATT, PT, D-D, and elevated FIB compared to the CCT group. The study suggested that the FIB value was higher in COVID-19 patients than that in the health group, especially for patients with thrombotic disease. The PT, APTT, D-D, and TT were related to the disease severity [22]. Some studies have shown that the TEG group reduced the consumption of blood products in the surgical surgery and severe coagulopathy [23–25], which were similar to our study, and the usage of RBC suspension, fresh-frozen plasma, cryoprecipitate and apheresis platelet in the TEG group was significantly reduced compared to the CCT group, but a study about liver transplantation indicated that the TEG group did not reduce intraoperative blood product consumption [26]. There should be more data to support above outcome since liver transplantation and orthopedic surgery belong to different types. Blood involves plasma, RBC, WBC, and PLT which represent physiological and pathological conditions of the human body [27] According to the routine blood tests, this study revealed that there was an significant decline on the Hb and RBC and an increase on the WBC after surgery, but the WBC was lower, Hb and RBC were higher in the TEG group than those in the CCT group, and there was no obvious difference on PLT between the two groups, which suggested that the TEG group could reduce the incidence of inflammation and anemia caused by blood loss. Under the guidance of TEG, the liver function of the patients was less damaged. Our data indicated a lower growth on the ALT, AST, and TBIL and a lower decline on the ALB, representing the injury of the liver function. These findings were closer to the other COVID-19 experiments, which suggested that the severe patients were with higher growth on the ALT and AST and slight difference on TBIL between the severe patients and the mild patients [28], and 34% of patients are with lower ALB, and 6% of patients were with abnormal TBIL [29]. BUN is closely with the renal function, and the elevated BUN suggests impaired renal function [30]. Scr is widely used as a biomarker of the renal function in clinical [31], and the research found that the rise on Scr caused by drug administration might be related to the impairment on the renal function [32]. UA, excreted mostly by the kidney, might be an essential factor which relates to the acute kidney injury, chronic kidney disease, and hypertension [33]. This study indicated that there was an increase on the levels of BUN and Scr, with a decline on the level of UA in the two groups after surgery, but the levels of BUN and Scr were lower, and the level of UA was higher in the TEG group than those in the CCT group, which implied the TEG-guided transfusion therapy prevented renal dysfunction. Besides the above advantages mentioned in this paper, there are some data limitations, such as the lack of adverse reactions and mortality after blood transfusion. Further studies should be carried out to ensure its efficacy.In summary, this study suggests that the TEG-guided transfusion strategy is superior to CCT-guided during orthopedic surgery, since TEG-guided transfusion therapy could reduce use of blood products, optimize blood components, and improve coagulation function for patients undergoing orthopedic surgery. Importantly, TEG-guided transfusion therapy may prevent liver and renal dysfunction after orthopedic surgery. However, this study was a noninterventional, observational study and did not have the capacity to allow outcome-based comparisons to determine the clinical value of TEG over CCTs or assess TEG as a predictor of bleeding or thrombosis. Therefore, further investigation with larger sample size is required.



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*Source: 8218042-2021-06-28.xml*