# Comparison of18F-NaF PET/CT and 18F-FDG PET/CT for Detection of Skull-Base Invasion and Osseous Metastases in Nasopharyngeal Carcinoma

Authors: Yin Zhang; Yue Chen; Zhanwen Huang; Li Zhang; Qiang Wan; Lei Lei
Journal: Contrast Media & Molecular Imaging
 (2018)

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## Abstract

Our study aimed at comparing the diagnostic value of18F-NaF positron emission tomography-computed tomography (PET/CT) and 18F-fluorodeoxyglucose (FDG) PET/CT for detection of skull-base invasion and osseous metastases in patients with nasopharyngeal carcinoma (NPC). Our study retrospectively analyzed 45 patients with pathologically proven NPC. They all underwent both 18F-NaF PET/CT and 18F-FDG PET/CT within a 7-day interval. Bone metastases were confirmed by follow-up using PET/CT, enhance-contrast computed tomography (CT), and magnetic resonance image (MRI). These two examinations were compared using per-patient-based analysis and per-lesion-based analysis. 18F-NaF PET/CT detected 27 patients with skull-base invasion, whereas 18F-FDG PET/CT detected 17 patients. 18F-NaF PET/CT and 18F-FDG PET/CT differed significantly in diagnosing skull-base invasion (p=0.02) and sensitivity (p=0.008). The sensitivity, specificity, and agreement rate of 18F-NaF PET/CT for detecting bone metastatic lesions were 98.3%, 65.7%, and 92.9%, respectively; these values were 42.9%, 97.1%, and 51.9%, respectively, for 18F-FDG PET/CT. 18F-NaF PET/CT and 18F-FDG PET/CT differed significantly in the number of osseous metastases detected (t=2.45, p=0.18) sensitivity (p<0.0001) and specificity (p=0.003). In patients with nasopharyngeal carcinoma, 18F-NaF PET/CT assessed invasion of the skull base better and detected more osseous metastases than 18F-FDG PET/CT.


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## Body

## 1. Introduction

Nasopharyngeal carcinoma (NPC) is an uncommon cancer worldwide but is prevalent in East and Southeast Asia [1]. NPC has a tendency to spread early to local sites, and regional nodal involvement is frequent (70–90%). Autopsy studies show that distant metastases are as frequent as 38–87% and that bone metastases occur in 70–80% of patients with distant metastases [2, 3]. The actual frequency of such metastases may be greater than the reported data owing to the low autopsy rate in Asia. Early and accurate NPC staging is important for improving both patient quality of life and therapeutic effects.Prior to treating NPC, the presence of bone metastases or skull-base invasion should be evaluated. The management of patients with osseous metastases is quite different. If skull-base invasion is diagnosed, the T-stage is upgraded to T3, which has implications for changing therapeutic strategies, such as increasing the radiation dose and extending the therapeutic field [4].99mTc-methylene diphosphonate (MDP) planar bone scan or single-photon emission computed tomography (SPECT) is widely used as noninvasive methods for detecting osseous metastases. However, these methods cannot obtain cross-sectional images of all the lesions, and they have lower resolution than other imaging techniques, such as positron emission tomography/computed tomography (PET/CT) [5].As a molecular imaging technology, PET/CT can indicate the degree of metabolic function of a malignancy and the clinical stage, response to therapy, and tumour recurrence, whereas conventional imaging modalities can only reveal morphological and anatomical information [6, 7]. 18F-fluorodeoxyglucose (FDG) has become a routine tracer agent for PET/CT but detecting osseous metastases is a relative weakness of 18F-FDG PET/CT compared with traditional bone scans and 18F-NaF PET/CT.18F-NaF was approved by the U.S. Food and Drug Administration as a bone-seeking diagnostic molecular imaging agent in 1972 [8]. Because 18F-NaF has better pharmacokinetic characteristics than 99mTc-MDP, 18F-NaF regained clinical attention with the development of PET/CT. Many reports have compared the diagnostic value of 18F-NaF PET/CT with that of 18F-FDG PET/CT for detecting osseous metastases of lung, breast, and prostate cancer [9, 10]. However, to the best of our knowledge, no study has compared the clinical value of 18F-NaF PET/CT with that of 18F-FDG PET/CT for staging NPC.


## 2. Materials and Methods

### 2.1. Patients

We reviewed the medical records of patients with pathologically proven NPC from March 2013 to June 2015 who underwent both18F-NaF PET/CT and 18F-FDG PET/CT within an interval of 7 days. The exclusion criteria were a history or the detection of another cancer type and an interval greater than 30 days between an imaging examination and chemotherapy or radiotherapy.We obtained informed consent from patients before both examinations. Our retrospective review of imaging studies was approved by the institutional review board of the Affiliated Hospital of Southwest Medical University.


### 2.2. The18F-FDG PET/CT and 18F-NaF PET/CT Protocols

18F-FDG and 18F-NaF were produced by a Cyclotron (Siemens Eclipse RD) and an automatic synthesis module (Beijing PET Technology Co., Ltd., Beijing, China) in our centre. The radiochemical purity of 18F-FDG was greater than 95%. Patients were requested to fast for at least 6 hours before 18F-FDG was administered. The 18F-FDG PET/CT procedure was delayed in patients with a blood glucose level >11 mmol/L (200 mg/dL) until the blood glucose level decreased to ≤11 mmol/L or these patients underwent the examination on another day [11, 12]. Before and after the injection, the patients rested and were kept quiet. The doses of 18F-FDG and 18F-NaF were 5.55 MBq/kg and 4.07 MBq/kg, respectively.Approximately 1 hour after the injection, examinations began with a Philips Gemini TF/16 PET/CT scanner. For18F-FDG PET/CT, CT scanning was first performed with 120 kV, 80–250 mA, 0.81 pitch, and 0.5 rotation time from the mid-thigh to the skull base. For 18F-NaF PET/CT, the scanned area ranged from the feet to the cranium. The emission image acquisition time was 70 seconds per bed position. PET image data were reconstructed by applying attenuation correction based on the CT data using the ordered subset expectation maximization algorithm.


### 2.3. Image Interpretation

Two experienced nuclear medicine physicians independently evaluated the18F-NaF PET/CT and 18F-FDG PET/CT images in a random order for each patient. They were blinded to other imaging results and the final results of the lesions. For discrepant cases, the interpreters reached a consensus.


### 2.4. Definition of Skull-Base Invasion and Metastases

PET component: local foci of the radioindicator were targeted as malignancy. The maximal standardized uptake value (SUVmax) of the mediastinal blood pool was considered as the reference value for 18F-FDG PET and the SUVmax of heterolateral or adjacent bone was the reference value for 18F-NaF PET. Because of the heterogeneity of 18F-NaF concentration in different bones, we could not establish a unified SUVmax standard to evaluate all skeletons [13].CT component: bone destruction or osteoblastic manifestation of bone (local and asymmetric lesions with increased density) was targeted as malignancy. Differentiations between osteogenic metastases, degenerative disease, and changes after radiotherapy, such as osteoradionecrosis, were difficult only by CT images [14]. Although the uptake was associated with the end plates or joint surfaces, it was always representative of degeneration disease [15]. We combined the clinical history with PET/CT features to make diagnosis.The examination range of18F-NaF PET/CT was from the feet to the cranium because of the clinical request of whole-body evaluation. But when we review the images, we only record the lesions located in the range from the mid-thigh to the skull base, which was same to 18F-FDG PET/CT. The final diagnosis was based on the overall findings from both the PET and CT components.Because the biopsy of all the skull-base invasion and bone metastases lesions were difficult to be obtained, whether the skull base of these patients were invaded was verified by MRI or contrast-enhanced CT within one week after the PET/CT examinations. Bone metastases were confirmed by enhance-contrast computed tomography (CT) or magnetic resonance image (MRI) and one year’s follow-up. If lesions progressed in the period of follow-up or osteolytic lesion changed to osteoblastic lesion during treatment, they were determined as bone metastases [16]. Undetermined lesions and lesions without obvious changes during follow-up were considered benign lesions (verified negatives) in the analysis.


### 2.5. Biochemical Analysis

Biochemical markers such as alkaline phosphatase were reported to be correlated with bone metastases [17]. Test data of serum alkaline phosphatase were collected if the interval time between blood test and 18F-NaF PET/CT was less than 7 days.


### 2.6. Statistical Analysis

The sensitivity, specificity, and positive and negative predictive values (PPVs and NPVs) of18F-NaF PET/CT and 18F-FDG PET/CT were calculated for the diagnosis of skull-base invasion (per-patient analysis) and the detection of bone metastases (per-lesion analyses). The number of osseous metastases detected by 18F-NaF PET/CT and 18F-FDG PET/CT were compared using the paired-samples t test. McNemar’s chi-squared test for matched pairs was used to compare the diagnostic value of 18F-NaF PET/CT with 18F-FDG PET/CT for detecting skull-base invasion. Serum alkaline phosphatase of patients with bone metastases and patients without bone metastases were compared using independent samples t-test. Correlation between serum alkaline phosphatase and SUVmax of 18F-NaF PET/CT had also been assessed by the Spearman analysis.


## 2.1. Patients

We reviewed the medical records of patients with pathologically proven NPC from March 2013 to June 2015 who underwent both18F-NaF PET/CT and 18F-FDG PET/CT within an interval of 7 days. The exclusion criteria were a history or the detection of another cancer type and an interval greater than 30 days between an imaging examination and chemotherapy or radiotherapy.We obtained informed consent from patients before both examinations. Our retrospective review of imaging studies was approved by the institutional review board of the Affiliated Hospital of Southwest Medical University.


## 2.2. The18F-FDG PET/CT and 18F-NaF PET/CT Protocols

18F-FDG and 18F-NaF were produced by a Cyclotron (Siemens Eclipse RD) and an automatic synthesis module (Beijing PET Technology Co., Ltd., Beijing, China) in our centre. The radiochemical purity of 18F-FDG was greater than 95%. Patients were requested to fast for at least 6 hours before 18F-FDG was administered. The 18F-FDG PET/CT procedure was delayed in patients with a blood glucose level >11 mmol/L (200 mg/dL) until the blood glucose level decreased to ≤11 mmol/L or these patients underwent the examination on another day [11, 12]. Before and after the injection, the patients rested and were kept quiet. The doses of 18F-FDG and 18F-NaF were 5.55 MBq/kg and 4.07 MBq/kg, respectively.Approximately 1 hour after the injection, examinations began with a Philips Gemini TF/16 PET/CT scanner. For18F-FDG PET/CT, CT scanning was first performed with 120 kV, 80–250 mA, 0.81 pitch, and 0.5 rotation time from the mid-thigh to the skull base. For 18F-NaF PET/CT, the scanned area ranged from the feet to the cranium. The emission image acquisition time was 70 seconds per bed position. PET image data were reconstructed by applying attenuation correction based on the CT data using the ordered subset expectation maximization algorithm.


## 2.3. Image Interpretation

Two experienced nuclear medicine physicians independently evaluated the18F-NaF PET/CT and 18F-FDG PET/CT images in a random order for each patient. They were blinded to other imaging results and the final results of the lesions. For discrepant cases, the interpreters reached a consensus.


## 2.4. Definition of Skull-Base Invasion and Metastases

PET component: local foci of the radioindicator were targeted as malignancy. The maximal standardized uptake value (SUVmax) of the mediastinal blood pool was considered as the reference value for 18F-FDG PET and the SUVmax of heterolateral or adjacent bone was the reference value for 18F-NaF PET. Because of the heterogeneity of 18F-NaF concentration in different bones, we could not establish a unified SUVmax standard to evaluate all skeletons [13].CT component: bone destruction or osteoblastic manifestation of bone (local and asymmetric lesions with increased density) was targeted as malignancy. Differentiations between osteogenic metastases, degenerative disease, and changes after radiotherapy, such as osteoradionecrosis, were difficult only by CT images [14]. Although the uptake was associated with the end plates or joint surfaces, it was always representative of degeneration disease [15]. We combined the clinical history with PET/CT features to make diagnosis.The examination range of18F-NaF PET/CT was from the feet to the cranium because of the clinical request of whole-body evaluation. But when we review the images, we only record the lesions located in the range from the mid-thigh to the skull base, which was same to 18F-FDG PET/CT. The final diagnosis was based on the overall findings from both the PET and CT components.Because the biopsy of all the skull-base invasion and bone metastases lesions were difficult to be obtained, whether the skull base of these patients were invaded was verified by MRI or contrast-enhanced CT within one week after the PET/CT examinations. Bone metastases were confirmed by enhance-contrast computed tomography (CT) or magnetic resonance image (MRI) and one year’s follow-up. If lesions progressed in the period of follow-up or osteolytic lesion changed to osteoblastic lesion during treatment, they were determined as bone metastases [16]. Undetermined lesions and lesions without obvious changes during follow-up were considered benign lesions (verified negatives) in the analysis.


## 2.5. Biochemical Analysis

Biochemical markers such as alkaline phosphatase were reported to be correlated with bone metastases [17]. Test data of serum alkaline phosphatase were collected if the interval time between blood test and 18F-NaF PET/CT was less than 7 days.


## 2.6. Statistical Analysis

The sensitivity, specificity, and positive and negative predictive values (PPVs and NPVs) of18F-NaF PET/CT and 18F-FDG PET/CT were calculated for the diagnosis of skull-base invasion (per-patient analysis) and the detection of bone metastases (per-lesion analyses). The number of osseous metastases detected by 18F-NaF PET/CT and 18F-FDG PET/CT were compared using the paired-samples t test. McNemar’s chi-squared test for matched pairs was used to compare the diagnostic value of 18F-NaF PET/CT with 18F-FDG PET/CT for detecting skull-base invasion. Serum alkaline phosphatase of patients with bone metastases and patients without bone metastases were compared using independent samples t-test. Correlation between serum alkaline phosphatase and SUVmax of 18F-NaF PET/CT had also been assessed by the Spearman analysis.


## 3. Results

In total, 45 patients were reviewed (Table1). All 45 patients were evaluated during a 3-month follow-up visit. 18F-NaF PET/CT detected skull-base invasion in the 26 patients with verified skull-base invasion. One additional patient who was diagnosed as having skull-base invasion according to 18F-NaF PET/CT was considered a false positive based on the MRI evaluation. In contrast, 18F-FDG PET/CT diagnosed only 17 of the 26 positively verified patients and did not detect any false-positive patients (Figure 1). Therefore, the sensitivity, specificity, accuracy, PPV, and NPV of 18F-NaF PET/CT for detecting skull-base invasion were 100%, 94.7%, 97.8%, 96.3%, and 100%, respectively, whereas these diagnostic measures were 65.4%, 100%, 80%, 100%, and 67.9%, respectively, for 18F-FDG PET/CT. 18F-NaF PET/CT correctly diagnosed more patients than 18F-FDG PET/CT (p=0.02). Whereas the sensitivity of 18F-NaF PET/CT was higher than that of 18F-FDG PET/CT (p=0.008), no significant difference in specificity was observed (p=1).Table 1
Demographic and clinical characteristics of study patients.
Characteristics
Number of patients (n=45)
%
Age
Range (22–73 years)
Median (52 years)
Gender
Male
36
80
Female
9
20
Histology
Squamous carcinoma
38
84.4
Undifferentiated carcinoma
7
15.6
Pretreatment
35
77.8
Posttreatment
10
22.2Figure 1
A 63-year-old man was diagnosed with nonkeratinizing nasopharyngeal carcinoma. (A–C) transverse sections of PET, CT, and fusion views in18F-FDG PET/CT, respectively. (D–F) transverse sections of PET, CT, and fusion views in 18F-NaF PET/CT. (G and H) the maximum intensity projection (MIP) of 18F-FDG PET/CT and 18F-NaF PET/CT, respectively. Skull-base invasion was revealed on 18F-NaF PET/CT but was hidden on 18F-FDG PET/CT because of the interference from the tumor tissue. This was consistent with MRI two days before 18F-NaF PET/CT.Osseous metastases were detected in 26 patients using18F-NaF PET/CT or 18F-FDG PET/CT. Using 18F-NaF PET/CT, 208 lesions were identified as bone metastases in 26 patients (mean, 8). In contrast, using 18F-FDG PET/CT, physicians diagnosed 81 lesions as osseous metastases (mean, 6.75). 18F-NaF PET/CT detected more bone metastatic lesions than 18F-FDG PET/CT did (t=2.45, p=0.018). The locations of these lesions are described in Table 2.Table 2
Description of osseous metastases detected in 26 patients: number of lesions by location, radiotracer, and follow-up status.
Location
NaF PET/CT
FDG PET/CT
Follow-up positive
Skull (except for skull base)
3
0
2
Sternum and ribs
52
17
46
Centrum
89
39
78
Ilium, pubis, and ischia
39
18
31
Limbs (include scapula and clavicle)
25
7
21
Total
208
81
177Over the course of more than one year’s follow-up, 43 patients underwent chest and abdominal CT or MRI examinations. Six patients completed whole-spine MRI scans. Five patients completed pelvic cavity CT or MRI examinations. Seven patients underwent PET/CT reexaminations and 15 patients underwent99mTc-MDP bone SPECT/CT scans. The final number of verified lesions was 212, among which 177 lesions were malignant and the other 35 lesions were benign. The osseous metastatic lesions that were diagnosed using 18F-NaF PET/CT and 18F-FDG PET/CT are presented in Table 3. Among the verified metastatic lesions, 12 lesions detected by 18F-NaF PET/CT were false positives, whereas 3 lesions were false negatives. In contrast, one lesion diagnosed by 18F-FDG PET/CT was a false positive, whereas 101 verified lesions were not detected by 18F-FDG PET/CT (Figure 2). The sensitivity, specificity, PPV, and NPV of 18F-NaF PET/CT and 18F-FDG PET/CT for the diagnosis of osseous metastatic lesions are presented in Table 4. The differences between 18F-NaF PET/CT and 18F-FDG PET/CT in sensitivity and specificity were both significant (p<0.0001 and p=0.003, respectively). Combining 18F-NaF PET/CT with 18F-FDG PET/CT changed 13 of 45 (28.9%) management decisions made by 18F-FDG PET/CT alone.Table 3
The proportion of confirmed osseous metastases that were detected by each radiotracer for each type of metastatic lesion.
18F-NaF PET/CT
18F-FDG PET/CT
Osteoblastic
42/50
18/18
Osteolytic
49/57
27/28
Mixed
22/22
8/8
No obvious abnormality on CT
61/79
23/27
Total
174/208
76/81Figure 2
A, D, and G are parts of18F-FDG PET/CT and C, F, and H are parts of 18F-NaF PET/CT. B and E are transverse sections of low-dose CT. Abnormal uptake of 18F-NaF is shown at the right rib and right ilium, whereas no abnormal concentration of 18F-FDG is found (arrows). The lesions are verified as osseous metastases by CT follow-up.Table 4
Measures of diagnostic performance using18F-NaF PET/CT or 18F-FDG PET/CT to detect osseous metastatic lesions of patients with nasopharyngeal carcinoma.
TP
FP
TN
FN
Sensitivity (%)
Specificity (%)
PPV (%)
NPV (%)
Accuracy (%)
18F-NaF PET/CT
174
12
23
3
98.3
65.7
93.4
88.5
92.9
18F-FDG PET/CT
76
1
34
101
42.9
97.1
98.7
25.2
51.9
TP: true positive; FP: false positive; TN: true negative; FN: false negative.40 patients underwent the blood test of near the18F-NaF PET/CT examinations. The range of serum alkaline phosphatase was between 5.5 and 128 U/L (median was 82.1 U/L). SUVmax of 18F-NaF PET/CT in these patients ranged from 8.16 to 68.8 (median was 16.9). T test showed there were no significant differences between patients with bone metastases and patients without bone metastases (t=1.575, p=0.124). There were no significant correlations between serum alkaline phosphatase and SUVmax of 18F-NaF PET/CT of these patients (rs=0.002, p=0.991).


## 4. Discussion

As revealed by Löfgren’s article [16], the pathological evidence of skull-base invasion and bone metastases was hard to be obtained even in the prospective study. It is mainly due to the impracticality of obtaining more than one, sometimes dozens of, biopsy specimens from one patient. Besides, the torture of patients and the difficulty of biopsy on skull-base invasion and bone metastases are other limitations to biopsy analyses. So the assessments of skull-base invasion and bone metastases are commonly accomplished by imaging methods.Imaging methods commonly used in the clinical staging of NPC include ultrasound, plain film, CT, MRI, bone scans, and PET/CT. These examinations are generally regional, except for bone scans and PET/CT. A whole-body examination using multiple imaging modalities is superior to evaluating the clinical stage using only regional scan methods. Ultrasound is inaccurate for assessing osseous status. Whole-body CT examination is limited by the radiation exposure. As for MRI, its disadvantage is the long examination time required.Among all imaging methods used for the management of cancer, the most specific one is radionuclide-labelled gene imaging. However, the target gene of NPC is under investigation [18, 19].In clinical practice, we use18F-FDG PET/CT as the common method for tumour staging. However, in 18F-FDG PET/CT, the uptake of radiotracer by brain and tumour tissue may disturb the estimation of whether the skull base is invaded. The advantages of 11C-choline PET/CT for T staging of NPC and other disease in skull base compared with 18F-FDG PET/CT have been reported [20, 21]. However, the difficulty of producing 11C-choline and the short half-life of the radionuclide are the limitations of its extension in clinical practice. These disadvantages are not applicable to 18F-NaF PET/CT.The uptake mechanism of18F-NaF is by chemisorption to hydroxyapatite, with resultant conversion into fluorapatite and a hydroxyl group. Regional blood flow and osteoblastic activity are main factors that influence the 18F-NaF uptake [15].The overall accuracy and sensitivity of18F-NaF PET/CT are superior to those of 18F-FDG PET/CT for diagnosing patients with skull-base invasion. Lau et al. previously reported that 18F-NaF PET/CT was more sensitive than 18F-FDG PET/CT for diagnosing skull-base invasion and could improve the diagnostic accuracy [4]. Our study revealed that 18F-NaF PET/CT was more sensitive than 18F-FDG PET/CT and exhibited a similar specificity. Owing to the better diagnostic performance of 18F-NaF PET/CT for evaluating the skull base, it can more accurately determine the target volume for radiotherapy.Although the reported false-positive rate of18F-NaF PET/CT is relatively high, our study demonstrated that the diagnostic accuracies of 18F-NaF PET/CT are sufficiently high for detecting skull-base involvement in patients with NPC while compared with MRI. This finding is consistent with our previous study [22]. We consider that this finding may be related to the false-positive discoveries of MRI owing to common oedema and inflammation before and after radiotherapy. Although the uptake of 18F-NaF is not specific to osseous malignancy, correlation of functional findings on 18F-NaF PET with anatomic information on CT improves the specificity of this modality. Further studies should be performed to compare the accuracies of 18F-NaF PET/CT, MRI, and true positive methods.18F-FDG PET/CT has advantages for evaluating systemic conditions. Liu et al. discovered that 18F-FDG PET can replace conventional work-ups, including chest radiography, abdominal ultrasonography, and skeletal scintigraphy, in the primary M staging of nonkeratinizing NPC [23]. However, a retrospective study of 35 newly diagnosed NPC patients conducted by Yang et al. found no significant difference between 18F-FDG PET/CT and planar bone scanning (PBS) in diagnosing one or more osseous metastases in NPC patients. They also reported that some bone metastases could be detected by PBS but not by 18F-FDG PET/CT [3]. Many studies have reported the superiority of 18F-NaF PET/CT for detecting bone metastases compared with 18F-FDG PET/CT [24–26]. Our study showed that in patients with NPC, 18F-NaF PET/CT detects more bone metastases with a higher sensitivity than 18F-FDG PET/CT does. For osteoblastic lesions, 18F-NaF PET/CT can show more sensitivity than 18F-FDG PET/CT due to the imaging mechanism of these two tracers. 18F-FDG PET/CT detects lesions owing to the abnormal metabolism of cancer cells, whereas 18F-NaF PET/CT reveals abnormal blood perfusion and bone reconstruction. Previous studies have shown that 18F-FDG PET/CT has modest sensitivity for detecting osteoblastic lesions and that 18F-NaF PET/CT detects both osteoblastic and osteolytic bone metastases well [10, 23]. In our study, 18F-NaF PET/CT detected more osteoblastic, osteolytic, and mixed-type metastases and lesions without obvious changes on the CT images compared with 18F-FDG PET/CT. These findings may be due to some osseous metastases having only abnormal blood perfusion or bone reconstruction without disordered glucose metabolism.Because18F-NaF PET/CT and 18F-FDG PET/CT each have unique advantages and disadvantages, medical management could be improved by using both methods in one combined examination [27]. Further study should be made to combine these two methods while keeping the radio-exposure of patients low enough.Serum alkaline phosphatase was proved to be uncorrelated with bone metastases and SUVmax of 18F-NaF PET/CT in our study. It may be due to the small sample and the huge amount of influence factors on serum alkaline phosphatase such as age and living standard. Even so, 18F-NaF PET/CT could still reflect the regional blood flow and osteoblastic activity in an noninvasive way, which could be an indicator for assessing treatment response [28].Our study has several limitations. First, our study was performed retrospectively with a limited number of patients who were heterogeneous, which might have led to selection bias. Second, it was impossible for us to obtain pathological material from each patient, which potentially produced errors in the final diagnosis. Third, in the benign group, we included undetermined lesions and lesions without obvious changes during follow-up, which may have increased the rate of false negatives.


## 5. Conclusion

This retrospective study of NPC patients demonstrated that18F-NaF PET/CT detected more osseous metastases and more accurately assessed skull-base invasion than did 18F-FDG PET/CT. Combining 18F-NaF PET/CT with 18F-FDG PET/CT could improve the stage evaluation of NPC compared with 18F-FDG PET/CT alone.



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