# The Impact of Seeding Density and Nitrogen Rates on Forage Yield and Quality ofAvena sativa L

Authors: Aroosa Kanwal; Dawood Zubair; Rao Mehboob ur Rehman; Muhammad Ibrahim; Muhammad Amjad Bashir; Muhammad Mudassar Maqbool; Muhammad Imran; Ubaid ur Rehman; Omaima Nasif; Mohammad Javed Ansari
Journal: BioMed Research International
 (2022)

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## Abstract

Green forage is an excellent feed source for livestock. It is an integral part of livestock production to accomplish the demands for butter, milk, and other derivatives for human utilization. Livestock contributes 11.39% towards the gross domestic product of Pakistan and 58.33% in agricultural farming. Livestock face shortage or insufficient supply of green fodder during the winter season, which ultimately reduces milk yield. Oat (Avena sativa L.) is a major forage crop in the winter season; however, several biotic and abiotic factors negatively affect its yields. Low soil fertility, particularly nitrogen deficiency, is regarded as one of the few reasons responsible for the low forage yield of oat. Low organic matter content in the soil, suboptimal agronomic practices, and harsh climatic conditions are the other major reasons for low oat yield. Seed rate and different nitrogen rates significantly alter green forage yield and quality of oat. This study assessed the impact of different seeding densities and nitrogen (N) doses on the forage yield of oat. Three seeding densities (70, 80, and 90 kg ha-1) and five N doses (0, 40, 80, 120, and 160 kg ha-1) were included in the study. The interactive effect of seeding density and N doses significantly altered green forage yield and quality attributes of oat. The highest green forage yield (54.67 t ha-1) was noted for the interaction among 90 kg seed rate ha-1 and 160 kg N ha-1. Similarly, the highest germination count (140 m-2), number of tillers (5.97 m-2), plant height (122.97 cm), number of leaves per plant (24.50 m-2), leaf area per tiller (123.18 cm2), fresh weight (5.47 kg m-2), dry weight (1692 g m-2), dry matter yield (20.90 t ha-1), crude protein (10.54%), crude fiber (31.62%), and total ash (9.39%) were recorded for the interactive effect of 90 kg seed rate ha-1 and 160 kg N ha-1. Economic analysis revealed that interaction between 90 kg seed rate ha-1 with 120 and 160 kg N ha-1 was superior to others with higher benefit: cost ratio and net economic returns. It is recommended that the oat seed rate of forage oat crop must be kept at 90 kg ha-1 and it should be supplied 120 kg N ha-1 for higher yield, better quality, and more economic returns.


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## Body

## 1. Introduction

Green forage is a valuable and the cheapest source of energy and provides excellent feed for livestock. A sustainable supply of green forage is a major constraint in livestock production to achieve the requirements for milk, butter, and other milk derivatives for human consumption [1]. Livestock contributes 58.33% towards agriculture and 11.39% towards the gross domestic product of Pakistan during 2016-2017 [2]. Livestock is usually underfed in Pakistan, which results in a low animal population. Imbalanced and low soil fertility, low organic matter content, and nitrogen deficiency are the major factors responsible for the low forage yield globally [3].Oat is locally known as “jai” or “jodar” in Pakistan and belongs to the family Poaceae. Pakistan is facing a 52-54% deficiency in the domestic fodder requirements [4]. Globally, oat is grown for grain, green forage, and fodder for livestock. It is the most important and cheapest source of cereal fodder crops grown during the winter season throughout Pakistan under rain-fed and irrigated conditions. Oat fodder is nutritious, palatable, and succulent. The nutritive value of oat fodder can be increased by combining it with legumes, like alfalfa, Persian clover, berseem, and pea [5]. It contains high amounts of minerals, including phosphorus and iron, fat, vitamin B1, and protein. Oat is a high-yielding crop in temperate climates and exhibits low tolerance to waterlogging [1]. Oat grains are a rich nutritive feed for dairy cows, sheep, horses, and young breeding animals [6]. Oat forage contains 30.44% crude fiber, 9.3% crude protein, 3.56% fat, and 0.27% phosphorus. It can be directly grazed to feed animals before seed setting and can be grown for grain purposes [7]. Its good quality grains and leaves are a rich source of carotene and carbohydrates. Oat requires 16-32°C temperature and 400 mm rainfall during the growing season for optimum growth and development [8].Grains and leaves of forage oat are a rich source of carotene and carbohydrates [7]. The forage yield of oat in Pakistan is too low than other countries. The main reasons for low forage production are changing climate, low soil fertility, unavailability of high-yielding varieties, socioeconomic factors, shortage of irrigation water, poor seeding techniques, and mismanagement of fertilizer application [9].GenusAvena consists of seventy species. Avena byzantina and Avena sativa are mainly cultivated for green forage and fodder purposes. There is a dire need to improve the forage yield of oat, which can be achieved by adopting improved agronomic practices [8]. Oat ranks 6th as a cereal crop worldwide after wheat, maize, rice, barley, and sorghum. Oat is a multicut fodder crop and achieves maximum green fodder yield with appropriate management. It should be harvested at 50% flower blooming [10].Sowing fodder crops with optimum seed rate is important to get sufficient plant population, which ultimately contributes towards high forage production. Plant population has a direct impact on forage yield and quality. Low and high plant population reduces the yield and quality of forages; thus, seeding density must be kept optimum. The seed rate of legumes could be decreased when these are sown in a mixture with other fodders [11, 12] The use of low or high seed rate exerts negative impacts on forage yield and quality [13]. A lower seed rate increases plant height, while a high seed rate reduces plant height due to less space, antagonism for light, and other resources [14]. The plant height of forage crops decreases with increasing seeding rate, which indicates competition for light [14].Kakol et al. [15] recorded the highest green forage yield of oat with a 100 kg ha-1 seed rate compared to 125 kg ha-1, while the quality of forage remained unaffected. Jan and Jan [16] have also reported a nonsignificant impact of seed rates on green and dry forage yields of oat. Abate and Wegi [13] concluded that optimum seed rate and fertilizer level have a significant effect on green forage yield of oat and dry matter production.Nitrogen (N) is a compulsory part of protein and a physiologically important compound that improves the growth and development of crop plants [17]. Nitrogen plays a vital role in crop production [18–20]. It is an essential ingredient of plant cell constituents like green pigments, amino acids, enzymes, and nucleic acids. Plants uptake N in dissolved form and partition it into different organs. Nitrogen exerts significant impacts on tillering, stem elongation, heading, cell division, booting, and grain filling. Nitrogen also affects crop morphology [21]. It is the most deficient nutrient in soils, thus required in heavy amounts for cereal and fodder crops [22]. Several factors including soil pH, moisture contents, and temperature significantly affect N losses [23]. However, the application of optimum dose is important to fetch high yield and quality [16]. Higher N application improves forage yield. Green fodder yield of oat was significantly affected by 80 kg N ha-1, and it was higher than control, 40 and 120 kg ha-1 [24]. However, the optimum N dose significantly varies among locations and agroclimatic conditions. Therefore, it is mandatory to optimize the N application dose and seed rate for high forage production. It was hypothesized that increasing the N dose will significantly differ the forage yield and quality. Similarly, different seed rates would have a significant impact on forage yield and quality of oat. The results will help to optimize seed rate and N doses for oat fodder production in agroclimatic conditions of Dera Ghazi Khan, Pakistan.


## 2. Materials and Methods

### 2.1. Experimental Site

The current field study to optimize seed rate and N application rate for oat were conducted at a research farm, Ghazi University, Dera Ghazi Khan, Pakistan, during the winter season, 2015-2016.


### 2.2. Experimental Details

The experiment was conducted on a fallow field, which was leveled, and fallow cultivation was done. Thereafter, presocking irrigation of 10 cm was applied, and the field was cultivated two times with the help of a cultivator followed by planking when the soil attained a workable moisture regime. The approved oat cultivar for forage production (S-2000) was used in the experiment. Three seed rates, i.e., 70, 80, and 90 kg ha-1, and five N levels (0, 40, 80, 120, and 160 kg ha-1) were included in the study. Seeds were sown in 30 cm-apart rows with the help of a single-row hand drill, and each experimental unit consisted of six lines. The crop was sown during the 2nd week of December 2015. Urea and single super phosphate (SSP) were used as the source of nitrogen and phosphorus, respectively. The whole amount of recommended phosphorus rate (80 kg ha-1) was applied as a basal dose, while N was applied in two splits according to the treatments. The first split of N was applied at the time of sowing, whereas the second split was given with the first irrigation. Three irrigations were given during the entire growth period of the crop. The crop was harvested manually at a ground level with the help of a sickle date.


### 2.3. Data Collection

Standard procedures were used for data collection which were kept uniform for all treatments. Data relating to germination count (m-2), plant height (cm), number of leaves (per plant), number of tillers (m-2), leaf area per tiller (cm2), fresh weight (kg m-2), dry weight (g m-2), green forage yield (t ha-1), and dry matter yield (t ha-1) were collected. For seed germination, experimental plots were visited daily until the last seed emerged. The number of seeds germinated on the final day of the count was regarded as germination count. The heights of five randomly selected plants from each experimental unit were measured and averaged. The number of tillers from five randomly selected plants in each experimental unit was counted and averaged. The destructive sampling method was used for the determination of fresh and dry biomass. A 1 m2 area was harvested and weighed to record fresh forage yield. The harvested sample was dried in an oven at 70°C, and then the dry yield was measured. This yield was then converted to t ha-1 by a unitary method. Crude protein (%), crude fiber (%), and total ash (%) were determined by burning a predefined quantity of the plants.


### 2.4. Economic Analysis

Economics analysis was conducted to determine the economic feasibility of applied treatments. Total and gross incomes were calculated from the total yield of the forage oat. Then the total cost of production was calculated by adding total fixed and total variable costs. Benefit-cost was determined by dividing the gross income by the total cost according to the procedures devised by CIMMYT (1988).(1)Benefit−costratio=NetincomeTotalexpenditure.


### 2.5. Statistical Analysis

The collected data of all parameters were analyzed by Fisher‘s analysis of the variance technique, and the LSD test at a 0.05 probability level was applied to compare the significance of treatment means [25].


## 2.1. Experimental Site

The current field study to optimize seed rate and N application rate for oat were conducted at a research farm, Ghazi University, Dera Ghazi Khan, Pakistan, during the winter season, 2015-2016.


## 2.2. Experimental Details

The experiment was conducted on a fallow field, which was leveled, and fallow cultivation was done. Thereafter, presocking irrigation of 10 cm was applied, and the field was cultivated two times with the help of a cultivator followed by planking when the soil attained a workable moisture regime. The approved oat cultivar for forage production (S-2000) was used in the experiment. Three seed rates, i.e., 70, 80, and 90 kg ha-1, and five N levels (0, 40, 80, 120, and 160 kg ha-1) were included in the study. Seeds were sown in 30 cm-apart rows with the help of a single-row hand drill, and each experimental unit consisted of six lines. The crop was sown during the 2nd week of December 2015. Urea and single super phosphate (SSP) were used as the source of nitrogen and phosphorus, respectively. The whole amount of recommended phosphorus rate (80 kg ha-1) was applied as a basal dose, while N was applied in two splits according to the treatments. The first split of N was applied at the time of sowing, whereas the second split was given with the first irrigation. Three irrigations were given during the entire growth period of the crop. The crop was harvested manually at a ground level with the help of a sickle date.


## 2.3. Data Collection

Standard procedures were used for data collection which were kept uniform for all treatments. Data relating to germination count (m-2), plant height (cm), number of leaves (per plant), number of tillers (m-2), leaf area per tiller (cm2), fresh weight (kg m-2), dry weight (g m-2), green forage yield (t ha-1), and dry matter yield (t ha-1) were collected. For seed germination, experimental plots were visited daily until the last seed emerged. The number of seeds germinated on the final day of the count was regarded as germination count. The heights of five randomly selected plants from each experimental unit were measured and averaged. The number of tillers from five randomly selected plants in each experimental unit was counted and averaged. The destructive sampling method was used for the determination of fresh and dry biomass. A 1 m2 area was harvested and weighed to record fresh forage yield. The harvested sample was dried in an oven at 70°C, and then the dry yield was measured. This yield was then converted to t ha-1 by a unitary method. Crude protein (%), crude fiber (%), and total ash (%) were determined by burning a predefined quantity of the plants.


## 2.4. Economic Analysis

Economics analysis was conducted to determine the economic feasibility of applied treatments. Total and gross incomes were calculated from the total yield of the forage oat. Then the total cost of production was calculated by adding total fixed and total variable costs. Benefit-cost was determined by dividing the gross income by the total cost according to the procedures devised by CIMMYT (1988).(1)Benefit−costratio=NetincomeTotalexpenditure.


## 2.5. Statistical Analysis

The collected data of all parameters were analyzed by Fisher‘s analysis of the variance technique, and the LSD test at a 0.05 probability level was applied to compare the significance of treatment means [25].


## 3. Results

### 3.1. Germination Count (m-2)

The germination count of forage oat was significantly affected by different seed rates, while the main effects of the N level were nonsignificant (Table1). Similarly, the interactive effect of the seed rate and N level was also nonsignificant. The highest germination count was recorded for S3 (138.77 m-2), whereas the lowest plant population (108.42 m-2) was noted for S1 (Table 2). The higher germination count is directly linked to a higher seed rate used.Table 1
Analysis of variance of different seeding rates, nitrogen doses, and their interactions on germination count, plant height, number of tillers per plant, leaf area per tiller, and fresh and dry biomass of oat.
SOV
DF
SS
MS
P value
Germination count
Seed rate (S)
2
6932.5
3466.25
0.0000∗∗
Nitrogen doses (N)
4
994.2
248.56
0.0852NS
S
×
N
8
1168.6
146.08
0.2640NS
Plant height
Seed rate (S)
2
359.69
179.85
0.0028∗
Nitrogen doses (N)
4
4829.57
1207.39
0.000∗∗
S
×
N
8
569.46
71.18
0.0173∗
Number of tillers
Seed rate (S)
2
9.9613
4.98067
0.0000∗∗
Nitrogen doses (N)
4
25.3444
6.33611
0.0000∗∗
S
×
N
8
2.5542
0.31928
0.00136∗
Leaf area per tiller
Seed rate (S)
2
1570.23
785.114
0.0000∗∗
Nitrogen doses (N)
4
2863.32
715.831
0.0000∗∗
S
×
N
8
883.81
110.476
0.0235∗
Fresh weight
Seed rate (S)
2
3.4964
1.74822
0.0000∗
Nitrogen doses (N)
4
32.3658
8.09144
0.0000∗∗
S
×
N
8
0.1969
0.02461
0.0240∗
Dry weight
Seed rate (S)
2
287097
143548
0.0008∗
Nitrogen doses (N)
4
7122222
1780555
0.0000∗∗
S
×
N
8
534114
66764
0.0016∗
SOV: source of variation, DF: degree of freedom, SS: sum of squares, MS: mean squares,∗: significant, NS: nonsignificant.Table 2
Interactive effect of seed rate and nitrogen level on germination count, plant height, numbers of leaves, and leaf area per tiller of forage oat.
Treatments
Germination count (m-2)
Plant height (cm)
Number of leaves (m-2)
Leaf area per tiller (cm2)
Fresh weight (kg m-2)
Dry weight (g m-2)
Seed rate (S)
S1
108.42 C
104.45 B
20.180 B
110.08 B
3.8267 C
897.7 B
S2
125.19 B
108.1 AB
20.780 B
118.32 A
4.2200 B
845.3 B
S3
138.77 A
111.37 A
22.060 A
100.02 C
4.5067 A
1034.7 A
LSD
3.8
1.8
0.32
2.33
0.34
45.13
Nitrogen doses (N)
N1
119.93 NS
92.51 D
19.667 D
107.00 B
2.7889 E
457.6 C
N2
118.64
103.48 C
17.756 E
117.81 A
3.7444 D
468.1 C
N3
123.08
105.32 C
20.956 C
121.95 A
4.3333 C
1008.9 B
N4
128.47
116.71 B
22.756 B
94.91 de
4.9556 B
1342.0 A
N5
130.61
121.90 A
23.900 A
96.54 de
5.1000 A
1353.0 A
LSD 0.05
4.91
2.33
0.42
90.54 e
0.44
58.26
S
×
N
S1N1
109.97 NS
94.43 g
17.50 g
115.91 ab
2.47 k
403.0 e
S1N2
103.23
94.37 g
17.67 fg
121.61 a
3.23 h
459.7 e
S1N3
101.70
96.33 fg
20.63 d
102.15 cd
4.03 fg
962.7 cd
S1N4
115.97
115.70 abc
22.20 c
104.71 cd
4.63 d
1317.3 b
S1N5
111.23
121.40 ab
22.90 bc
104.58 cd
4.77 d
1346.0 b
S2N1
111.2
91.37 g
17.43 g
115.78 ab
2.87 j
494.0 e
S2N2
114.53
104.50 ef
19.10 ef
123.18 a
3.90 g
470.7 e
S2N3
129.13
106.30 de
20.03 de
107.38 bc
4.33 e
1167.3 bc
S2N4
131.23
115.57 abc
23.03 abc
121.54 a
4.93 c
1016.7 cd
S2N5
139.87
122.97 a
24.30 ab
119.88 a
5.07 c
1348.3 b
S3N1
138.33
91.73 g
18.33 fg
121.74 a
3.03 i
475.7 e
S3N2
138.17
111.57 cde
22.23 c
121.08 a
4.10 f
474.0 e
S3N3
138.4
113.33 bcd
22.20 c
10.65
4.63 d
896.7 d
S3N4
138.2
118.87 abc
23.03 abc
110.77
5.30 b
1692.0 a
S3N5
140.73
121.33 ab
24.50 a
5.47 a
1364.7 b
LSD 0.05
17.44
8.29
1.48
0.16
206.7
Mean
124.13
107.98
21
4.18
19.32
Here,S1=70kgha−1, S2=80kgha−1, S3=90kgha−1, N1=0kgha−1, N2=40kgha−1, N3=80kgha−1, N4=120kgha−1, and N5=160kgha−1. Means sharing similar letters within a column are statistically nonsignificant.


### 3.2. Morphological Attributes

The individual and interactive effects of seed rate and N doses significantly altered plant height, number of leaves per plant, leaf area per tiller, and fresh and dry weight (Table1). The highest plants were observed for S2N5 (122.97 cm), which was statistically at par with S1N5, S3N5, S3N4, and S1N4. The lowest plant height was observed for S2N1 (91.37 cm), S1N2 (94.37 cm), S1N1 (94.43 cm), and S1N3 (96.33 cm). The increase in N unit increased plant height (Table 2).More number of leaves of forage oat were noted for S3N5 (24.50 m-2), which was statistically similar to S2N5, S3N4, and S2N4. The lowest numbers of leaves per plant were recorded for S2N1, which was statistically similar to S1N1, S1N2, and S3N1 (Table 2).The highest leaf area was recorded for S2N5, which was statistically similar to S3N4, S1N5, S3N2, and S3N5. The lowest leaf area was observed for S1N1, and it was closely related to S1N2 and S1N3 (Table 2).


### 3.3. Yield and Quality Attributes

The individual and interactive effects of seed rate and N doses significantly affected forage yield, dry matter yield, crude fiber, crude protein, and total ash in forage oat (Table3). Overall, these parameters were increased with increasing seed rate and N levels. The highest green forage yield was noted for S3N5, which was similar to S3N4, S2N5, and S2N4 (Table 4). The lowest green forage yield was produced by S1N1, and it was statistically similar to S2N1 and S3N1. Kakol et al. (2003) reported that oat plants positively respond to seed rate and N, which improve their growth and forage production. Shukla and Lal (1998) recorded significant differences among 80 and 60 kg phosphors for green forage yield. The highest dry matter yield was observed for S1N4, whereas S1N1 recorded the lowest dry matter yield (Table 4).Table 3
Analysis of variance of different seeding rates, nitrogen doses, and their interactions on forage and dry matter yields, crude fiber, crude protein, and total ash of forage oat.
SOV
DF
SS
MS
P value
Forage yield
Seed rate (S)
2
338.71
169.356
0.0000∗∗
Nitrogen doses (N)
4
3213.35
803.338
0.0000∗∗
S
×
N
8
28.89
3.612
0.0151∗
Dry matter yield
Seed rate (S)
2
3.7773
1.8887
0.0008∗
Nitrogen doses (N)
4
83.3142
20.8286
0.0000∗∗
S
×
N
8
4.2604
0.5326
0.0268∗
Crude protein
Seed rate (S)
2
1.2379
0.6189
0.0304∗
Nitrogen doses (N)
4
72.2770
18.0692
0.0000∗∗
S
×
N
8
6.8236
0.8529
0.0003∗
Crude fiber
Seed rate (S)
2
53.324
26.6618
0.0037∗
Nitrogen doses (N)
4
226.558
56.6395
0.0000∗∗
S
×
N
8
97.847
12.2309
0.0111∗
Total ash
Seed rate (S)
2
9.3352
4.66760
0.0000∗∗
Nitrogen doses (N)
4
11.2192
2.80480
0.0000∗∗
S
×
N
8
4.8808
0.61010
0.0029∗
SOV: source of variation, DF: degree of freedom, SS: sum of squares, MS: mean squares,∗: significant, NS: nonsignificant.Table 4
Interactive effect of seed rate and nitrogen level on green forage yield, dry matter yield, crude protein, crude fiber, and total ash of forage oat.
Treatments
Green forage yield (t ha-1)
Dry matter yield (t ha-1)
Crude protein (%)
Crude fiber (%)
Total ash (%)
Seed rate (S)
S1
38.827 C
19.07 B
8.87 A
27.28 B
7.58 C
S2
42.717 B
19.16 B
8.52 A
27.10 B
7.91 B
S3
45.517 A
19.73 A
8.88 B
29.49 A
8.67 A
LSD 0.05
0.4
0.16
0.14
0.72
0.14
Nitrogen doses (N)
N1
28.389 E
17.17 D
6.98 E
24.75 C
7.14 C
N2
37.978 D
18.57 C
7.82 D
26.76 B
7.93 B
N3
43.983 C
19.40 B
8.72 C
27.36 B
8.30 AB
N4
50.170 B
20.64 A
9.86 B
29.88 A
8.44 A
N5
51.249 A
20.87 A
10.42 A
31.03 A
8.48 A
LSD 0.05
0.51
0.21
0.19
0.92
0.18
S
×
N
S1N1
25.833 i
16.60 d
7.45 ef
24.55 d
6.57 e
S1N2
32.600 g
18.07 c
8.23 d
24.71d
7.02 e
S1N3
41.033 ef
19.33 b
8.41 d
27.75 bcd
8.00 cd
S1N4
46.667 c
20.57 a
9.80 bc
29.32 abc
7.88 cd
S1N5
48.000 c
20.80 a
10.45 ab
30.05 ab
8.45 bc
S2N1
29.000 h
16.70 d
5.94 g
20.55 e
6.78 e
S2N2
39.667 f
18.23 c
7.22 f
26.22 cd
7.79 d
S2N3
43.917 d
19.43 b
9.27 c
27.15 bcd
8.45 bc
S2N4
49.923 b
20.63 a
9.93 abc
30.15 ab
8.05 cd
S2N5
51.080 b
20.80 a
10.28 ab
31.42 a
8.49 bc
S3N1
30.333 h
18.20 c
7.54 ef
29.15 abc
8.05 cd
S3N2
41.667 e
19.37 b
8.00 de
29.35 abc
8.99 ab
S3N3
47.000 c
19.43 b
8.49 d
27.18 bcd
8.45 bc
S3N4
53.920 a
20.90 a
9.85 bc
30.15 ab
9.39 a
S3N5
54.667 a
20.73 a
10.54 a
31.62 a
8.49 bc
LSD 0.05
1.84
0.75
0.66
3.29
0.65
Mean
42.35
19.32
8.76
27.95
8.06
Here,S1=70kgha−1, S2=80kgha−1, S3=90kgha−1, N1=0kgha−1, N2=40kgha−1, N3=80kgha−1, N4=120kgha−1, and N5=160kgha−1. Means sharing similar letters within a column are statistically nonsignificant.Variation in seed rate and N level significantly affected the crude fiber percentage. The highest crude fiber percentage was observed for S3N5, while S2N1 resulted in the lowest crude fiber percentage (Table 4).


### 3.4. Economic Analysis

The interactive effect of seed rate and N doses has a significant impact on variable costs (Table5) and economics (Table 6). The highest net income and benefit-cost ratio was recorded for S3N4, whereas S1N1 recorded the lowest net income and benefit-cost ratio, although increasing N doses improved all traits and the highest dose proved superior in this regard. However, the economic analysis revealed that applying higher N doses is not an economic option. Although the highest N dose, i.e., N5, also recorded a higher net income and benefit-cost ratio than other N doses, N4 recorded the highest net income and benefit-cost ratio (Table 6).Table 5
Fixed and variable costs of production for forage oat.
Operation/input
No. of operations/ha
Rate per unit (PKR)
Cost per unit (PKR)
Land preparation
Ploughing
2
2000 ha-1
4000 ha-1
Planking
1
1500 ha-1
1500 ha-1
Sowing
Seed/sowing
∗
∗
∗
Drill sowing
2500 ha-1
2500 ha-1
Irrigation
Irrigation charges
3 irrigations
1000
3000
Labor cost for irrigation
1 man for 3 times
500 per day
1500
Fertilizer
Nitrogen from urea
∗
∗
∗
Phosphorus from single super phosphate
80 kg
111 kg-1
8888
Plant protection measures
Weeding
1000
Harvesting
Labor charges for harvesting
7 men
500 per men
3500
Land rent
6 months
30250 ha-1
15125
Total fixed cost
41013
∗: variable cost of seed, ∗: variable cost of nitrogen.Table 6
Economics analysis for different seed rates and nitrogen doses used to produce forage oat.
Treatments
Gross income (PKR ha-1)
Total cost (PKR ha-1)
Net income (PKR ha-1)
Benefit : cost ratio
S1N1
51666
45213
6453
1.14
S1N2
65200
48253
16947
1.35
S1N3
82066
51293
30773
1.60
S1N4
93334
54333
39001
1.72
S1N5
96000
57373
38627
1.67
S2N1
58000
45813
12187
1.27
S2N2
79334
48853
30481
1.62
S2N3
87834
51893
35941
1.69
S2N4
99846
54933
44913
1.82
S2N5
102160
57973
44187
1.76
S3N1
60666
46413
14253
1.31
S3N2
83334
49453
33881
1.69
S3N3
94000
52493
41507
1.79
S3N4
107840
55533
52307
1.94
S3N5
109334
58573
50761
1.87
1
USD
=
160
PKR. Here, S1=70kgha−1, S2=80kgha−1, S3=90kgha−1, N1=0kgha−1, N2=40kgha−1, N3=80kgha−1, N4=120kgha−1, and N5=160kgha−1.


## 3.1. Germination Count (m-2)

The germination count of forage oat was significantly affected by different seed rates, while the main effects of the N level were nonsignificant (Table1). Similarly, the interactive effect of the seed rate and N level was also nonsignificant. The highest germination count was recorded for S3 (138.77 m-2), whereas the lowest plant population (108.42 m-2) was noted for S1 (Table 2). The higher germination count is directly linked to a higher seed rate used.Table 1
Analysis of variance of different seeding rates, nitrogen doses, and their interactions on germination count, plant height, number of tillers per plant, leaf area per tiller, and fresh and dry biomass of oat.
SOV
DF
SS
MS
P value
Germination count
Seed rate (S)
2
6932.5
3466.25
0.0000∗∗
Nitrogen doses (N)
4
994.2
248.56
0.0852NS
S
×
N
8
1168.6
146.08
0.2640NS
Plant height
Seed rate (S)
2
359.69
179.85
0.0028∗
Nitrogen doses (N)
4
4829.57
1207.39
0.000∗∗
S
×
N
8
569.46
71.18
0.0173∗
Number of tillers
Seed rate (S)
2
9.9613
4.98067
0.0000∗∗
Nitrogen doses (N)
4
25.3444
6.33611
0.0000∗∗
S
×
N
8
2.5542
0.31928
0.00136∗
Leaf area per tiller
Seed rate (S)
2
1570.23
785.114
0.0000∗∗
Nitrogen doses (N)
4
2863.32
715.831
0.0000∗∗
S
×
N
8
883.81
110.476
0.0235∗
Fresh weight
Seed rate (S)
2
3.4964
1.74822
0.0000∗
Nitrogen doses (N)
4
32.3658
8.09144
0.0000∗∗
S
×
N
8
0.1969
0.02461
0.0240∗
Dry weight
Seed rate (S)
2
287097
143548
0.0008∗
Nitrogen doses (N)
4
7122222
1780555
0.0000∗∗
S
×
N
8
534114
66764
0.0016∗
SOV: source of variation, DF: degree of freedom, SS: sum of squares, MS: mean squares,∗: significant, NS: nonsignificant.Table 2
Interactive effect of seed rate and nitrogen level on germination count, plant height, numbers of leaves, and leaf area per tiller of forage oat.
Treatments
Germination count (m-2)
Plant height (cm)
Number of leaves (m-2)
Leaf area per tiller (cm2)
Fresh weight (kg m-2)
Dry weight (g m-2)
Seed rate (S)
S1
108.42 C
104.45 B
20.180 B
110.08 B
3.8267 C
897.7 B
S2
125.19 B
108.1 AB
20.780 B
118.32 A
4.2200 B
845.3 B
S3
138.77 A
111.37 A
22.060 A
100.02 C
4.5067 A
1034.7 A
LSD
3.8
1.8
0.32
2.33
0.34
45.13
Nitrogen doses (N)
N1
119.93 NS
92.51 D
19.667 D
107.00 B
2.7889 E
457.6 C
N2
118.64
103.48 C
17.756 E
117.81 A
3.7444 D
468.1 C
N3
123.08
105.32 C
20.956 C
121.95 A
4.3333 C
1008.9 B
N4
128.47
116.71 B
22.756 B
94.91 de
4.9556 B
1342.0 A
N5
130.61
121.90 A
23.900 A
96.54 de
5.1000 A
1353.0 A
LSD 0.05
4.91
2.33
0.42
90.54 e
0.44
58.26
S
×
N
S1N1
109.97 NS
94.43 g
17.50 g
115.91 ab
2.47 k
403.0 e
S1N2
103.23
94.37 g
17.67 fg
121.61 a
3.23 h
459.7 e
S1N3
101.70
96.33 fg
20.63 d
102.15 cd
4.03 fg
962.7 cd
S1N4
115.97
115.70 abc
22.20 c
104.71 cd
4.63 d
1317.3 b
S1N5
111.23
121.40 ab
22.90 bc
104.58 cd
4.77 d
1346.0 b
S2N1
111.2
91.37 g
17.43 g
115.78 ab
2.87 j
494.0 e
S2N2
114.53
104.50 ef
19.10 ef
123.18 a
3.90 g
470.7 e
S2N3
129.13
106.30 de
20.03 de
107.38 bc
4.33 e
1167.3 bc
S2N4
131.23
115.57 abc
23.03 abc
121.54 a
4.93 c
1016.7 cd
S2N5
139.87
122.97 a
24.30 ab
119.88 a
5.07 c
1348.3 b
S3N1
138.33
91.73 g
18.33 fg
121.74 a
3.03 i
475.7 e
S3N2
138.17
111.57 cde
22.23 c
121.08 a
4.10 f
474.0 e
S3N3
138.4
113.33 bcd
22.20 c
10.65
4.63 d
896.7 d
S3N4
138.2
118.87 abc
23.03 abc
110.77
5.30 b
1692.0 a
S3N5
140.73
121.33 ab
24.50 a
5.47 a
1364.7 b
LSD 0.05
17.44
8.29
1.48
0.16
206.7
Mean
124.13
107.98
21
4.18
19.32
Here,S1=70kgha−1, S2=80kgha−1, S3=90kgha−1, N1=0kgha−1, N2=40kgha−1, N3=80kgha−1, N4=120kgha−1, and N5=160kgha−1. Means sharing similar letters within a column are statistically nonsignificant.


## 3.2. Morphological Attributes

The individual and interactive effects of seed rate and N doses significantly altered plant height, number of leaves per plant, leaf area per tiller, and fresh and dry weight (Table1). The highest plants were observed for S2N5 (122.97 cm), which was statistically at par with S1N5, S3N5, S3N4, and S1N4. The lowest plant height was observed for S2N1 (91.37 cm), S1N2 (94.37 cm), S1N1 (94.43 cm), and S1N3 (96.33 cm). The increase in N unit increased plant height (Table 2).More number of leaves of forage oat were noted for S3N5 (24.50 m-2), which was statistically similar to S2N5, S3N4, and S2N4. The lowest numbers of leaves per plant were recorded for S2N1, which was statistically similar to S1N1, S1N2, and S3N1 (Table 2).The highest leaf area was recorded for S2N5, which was statistically similar to S3N4, S1N5, S3N2, and S3N5. The lowest leaf area was observed for S1N1, and it was closely related to S1N2 and S1N3 (Table 2).


## 3.3. Yield and Quality Attributes

The individual and interactive effects of seed rate and N doses significantly affected forage yield, dry matter yield, crude fiber, crude protein, and total ash in forage oat (Table3). Overall, these parameters were increased with increasing seed rate and N levels. The highest green forage yield was noted for S3N5, which was similar to S3N4, S2N5, and S2N4 (Table 4). The lowest green forage yield was produced by S1N1, and it was statistically similar to S2N1 and S3N1. Kakol et al. (2003) reported that oat plants positively respond to seed rate and N, which improve their growth and forage production. Shukla and Lal (1998) recorded significant differences among 80 and 60 kg phosphors for green forage yield. The highest dry matter yield was observed for S1N4, whereas S1N1 recorded the lowest dry matter yield (Table 4).Table 3
Analysis of variance of different seeding rates, nitrogen doses, and their interactions on forage and dry matter yields, crude fiber, crude protein, and total ash of forage oat.
SOV
DF
SS
MS
P value
Forage yield
Seed rate (S)
2
338.71
169.356
0.0000∗∗
Nitrogen doses (N)
4
3213.35
803.338
0.0000∗∗
S
×
N
8
28.89
3.612
0.0151∗
Dry matter yield
Seed rate (S)
2
3.7773
1.8887
0.0008∗
Nitrogen doses (N)
4
83.3142
20.8286
0.0000∗∗
S
×
N
8
4.2604
0.5326
0.0268∗
Crude protein
Seed rate (S)
2
1.2379
0.6189
0.0304∗
Nitrogen doses (N)
4
72.2770
18.0692
0.0000∗∗
S
×
N
8
6.8236
0.8529
0.0003∗
Crude fiber
Seed rate (S)
2
53.324
26.6618
0.0037∗
Nitrogen doses (N)
4
226.558
56.6395
0.0000∗∗
S
×
N
8
97.847
12.2309
0.0111∗
Total ash
Seed rate (S)
2
9.3352
4.66760
0.0000∗∗
Nitrogen doses (N)
4
11.2192
2.80480
0.0000∗∗
S
×
N
8
4.8808
0.61010
0.0029∗
SOV: source of variation, DF: degree of freedom, SS: sum of squares, MS: mean squares,∗: significant, NS: nonsignificant.Table 4
Interactive effect of seed rate and nitrogen level on green forage yield, dry matter yield, crude protein, crude fiber, and total ash of forage oat.
Treatments
Green forage yield (t ha-1)
Dry matter yield (t ha-1)
Crude protein (%)
Crude fiber (%)
Total ash (%)
Seed rate (S)
S1
38.827 C
19.07 B
8.87 A
27.28 B
7.58 C
S2
42.717 B
19.16 B
8.52 A
27.10 B
7.91 B
S3
45.517 A
19.73 A
8.88 B
29.49 A
8.67 A
LSD 0.05
0.4
0.16
0.14
0.72
0.14
Nitrogen doses (N)
N1
28.389 E
17.17 D
6.98 E
24.75 C
7.14 C
N2
37.978 D
18.57 C
7.82 D
26.76 B
7.93 B
N3
43.983 C
19.40 B
8.72 C
27.36 B
8.30 AB
N4
50.170 B
20.64 A
9.86 B
29.88 A
8.44 A
N5
51.249 A
20.87 A
10.42 A
31.03 A
8.48 A
LSD 0.05
0.51
0.21
0.19
0.92
0.18
S
×
N
S1N1
25.833 i
16.60 d
7.45 ef
24.55 d
6.57 e
S1N2
32.600 g
18.07 c
8.23 d
24.71d
7.02 e
S1N3
41.033 ef
19.33 b
8.41 d
27.75 bcd
8.00 cd
S1N4
46.667 c
20.57 a
9.80 bc
29.32 abc
7.88 cd
S1N5
48.000 c
20.80 a
10.45 ab
30.05 ab
8.45 bc
S2N1
29.000 h
16.70 d
5.94 g
20.55 e
6.78 e
S2N2
39.667 f
18.23 c
7.22 f
26.22 cd
7.79 d
S2N3
43.917 d
19.43 b
9.27 c
27.15 bcd
8.45 bc
S2N4
49.923 b
20.63 a
9.93 abc
30.15 ab
8.05 cd
S2N5
51.080 b
20.80 a
10.28 ab
31.42 a
8.49 bc
S3N1
30.333 h
18.20 c
7.54 ef
29.15 abc
8.05 cd
S3N2
41.667 e
19.37 b
8.00 de
29.35 abc
8.99 ab
S3N3
47.000 c
19.43 b
8.49 d
27.18 bcd
8.45 bc
S3N4
53.920 a
20.90 a
9.85 bc
30.15 ab
9.39 a
S3N5
54.667 a
20.73 a
10.54 a
31.62 a
8.49 bc
LSD 0.05
1.84
0.75
0.66
3.29
0.65
Mean
42.35
19.32
8.76
27.95
8.06
Here,S1=70kgha−1, S2=80kgha−1, S3=90kgha−1, N1=0kgha−1, N2=40kgha−1, N3=80kgha−1, N4=120kgha−1, and N5=160kgha−1. Means sharing similar letters within a column are statistically nonsignificant.Variation in seed rate and N level significantly affected the crude fiber percentage. The highest crude fiber percentage was observed for S3N5, while S2N1 resulted in the lowest crude fiber percentage (Table 4).


## 3.4. Economic Analysis

The interactive effect of seed rate and N doses has a significant impact on variable costs (Table5) and economics (Table 6). The highest net income and benefit-cost ratio was recorded for S3N4, whereas S1N1 recorded the lowest net income and benefit-cost ratio, although increasing N doses improved all traits and the highest dose proved superior in this regard. However, the economic analysis revealed that applying higher N doses is not an economic option. Although the highest N dose, i.e., N5, also recorded a higher net income and benefit-cost ratio than other N doses, N4 recorded the highest net income and benefit-cost ratio (Table 6).Table 5
Fixed and variable costs of production for forage oat.
Operation/input
No. of operations/ha
Rate per unit (PKR)
Cost per unit (PKR)
Land preparation
Ploughing
2
2000 ha-1
4000 ha-1
Planking
1
1500 ha-1
1500 ha-1
Sowing
Seed/sowing
∗
∗
∗
Drill sowing
2500 ha-1
2500 ha-1
Irrigation
Irrigation charges
3 irrigations
1000
3000
Labor cost for irrigation
1 man for 3 times
500 per day
1500
Fertilizer
Nitrogen from urea
∗
∗
∗
Phosphorus from single super phosphate
80 kg
111 kg-1
8888
Plant protection measures
Weeding
1000
Harvesting
Labor charges for harvesting
7 men
500 per men
3500
Land rent
6 months
30250 ha-1
15125
Total fixed cost
41013
∗: variable cost of seed, ∗: variable cost of nitrogen.Table 6
Economics analysis for different seed rates and nitrogen doses used to produce forage oat.
Treatments
Gross income (PKR ha-1)
Total cost (PKR ha-1)
Net income (PKR ha-1)
Benefit : cost ratio
S1N1
51666
45213
6453
1.14
S1N2
65200
48253
16947
1.35
S1N3
82066
51293
30773
1.60
S1N4
93334
54333
39001
1.72
S1N5
96000
57373
38627
1.67
S2N1
58000
45813
12187
1.27
S2N2
79334
48853
30481
1.62
S2N3
87834
51893
35941
1.69
S2N4
99846
54933
44913
1.82
S2N5
102160
57973
44187
1.76
S3N1
60666
46413
14253
1.31
S3N2
83334
49453
33881
1.69
S3N3
94000
52493
41507
1.79
S3N4
107840
55533
52307
1.94
S3N5
109334
58573
50761
1.87
1
USD
=
160
PKR. Here, S1=70kgha−1, S2=80kgha−1, S3=90kgha−1, N1=0kgha−1, N2=40kgha−1, N3=80kgha−1, N4=120kgha−1, and N5=160kgha−1.


## 4. Discussion

The higher germination count is directly linked to a higher seed rate used. These results are similar to [26] who recorded the highest number of plants of forage maize with a higher seeding density. The nonsignificant effect of N doses on germination of forage oat is also reported by Shukla and Lal [27] who also reported nonsignificant results of oat germination percentage when grown with organic or inorganic sources of fertilizers. This might be due to the contribution of N in the growth of oat plants. Zahid et al. [28] concluded that plant height was increased with increasing N doses and farmyard manure. Irfan et al. [29] found a significant difference in the plant height of oat, and these results are also in line with others. Another concluded that the plant height of oat was significantly altered by the split application of N and potassium. One of them recorded significantly higher plant height (119.58 cm) in forage oat by the application of 80 kg N ha-1.The higher number of leaves per plant can be owed to the contribution of N in vegetative growth. Similar findings have been reported by Ahmad et al. [30]. Irfan et al. [29] reported higher and lower numbers of leaves of forage oat with the highest and the lowest N application.The highest number of tillers m-2 was counted for S3N5, which was similar to S3N4, S2N5, S3N3, and S3N2. The lowest number of tillers per plant in forage oat was observed for S1N1, which was statistically similar to S2N1, S3N1, and S1N2 (Table 2). Metwally et al. [31] concluded that the application of 100 kg ha-1 N significantly enhanced the tillering capacity of forage oat. Jehangir et al. [32] reported that the number of tillers significantly increased with increasing the fertility status of the soil. These results are in line with Ahmad et al. [30] who concluded that 150 kg ha-1 N and 60 kg ha-1 phosphorus produces the highest leaf area of (128 cm2) in forage oat. Jiwang et al. [33] reported that increasing the fertilizer level increases the leaf area. Khandaker and Islam [34] recorded the highest leaf area of forage oat with 120 kg ha-1 N. Tanha [35] observed the highest leaf area in forage maize with 200 kg ha-1 N application.Fresh and dry weights significantly increased with the increasing seed rate and N level. The highest fresh weight was observed for S3N5, which was close to S3N4. The lowest fresh weight was observed for S1N1. Sharma and Bhunia [36] studied the response of organic and inorganic sources of N and concluded that the inorganic N source produced the highest fresh weight per tiller. Singh et al. [37] reported that the application of farmyard manure significantly increased the fodder yield in maize. Wheed et al. [38] reported that a higher N level increased green forage yield.Orloff et al. [39] recorded the highest dry fodder yield with 136 kg ha-1 N application.Crude protein percentage is an important quality parameter, which determined the quality of forage crops. Application of N from lower to higher levels significantly increased crude protein contents; however, crude protein beyond a certain range reduced the forage quality and increases succulence. The highest crude protein was observed for S2N5, whereas S2N1 resulted in the lowest crude protein. Kumar et al. [40] observed that 80 kg ha-1 N resulted in the maximum crude protein yield and observed a maximum crude protein yield with 120 kg N ha-1. Kumar et al. [41] observed that the application of N up to 80 kg per hectare enhanced the crude protein yield, and a further increase in N decreased the crude protein yield. Khan et al. [42] concluded that crude protein quality may be affected by N application as it is an essential part of protein, chlorophyll, and protoplast. Farooq et al. [43] also reported similar results. These results are in line with [44] who observed higher crude fiber with 150 kg ha-1 N application.The highest ash percentage was recorded for S3N4, and the lowest was observed for S1N1 (Table 4). These results are in line with Alajmi et al. [45] who observed a maximum ash percentage with 150 kg ha-1 N application. Saleh et al. [46] also concluded similar results and reported that increasing N application significantly increased the ash content in forage maize.


## 5. Conclusion

The results revealed that seed rate and nitrogen doses significantly altered the yield and forage quality of forage oat. It is concluded that forage oat crops should be grown with a seed rate of 90 kg ha-1 and supplemented with 120 kg ha-1 of nitrogen for higher yield, better quality, and more economic returns.



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*Source: 8238634-2022-06-30.xml*