# Effect of Adding Chelating Agents on the Absorption of Zinc from Polluted Soil Sludge Textile Industrial Waste by Sunflower Plant (Helianthus annuus L.)

Authors: Ruslan Wirosoedarmo; F. Anugroho; S. D. Hanggara; Kiki Gustinasari
Journal: Applied and Environmental Soil Science
 (2018)

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## Abstract

Textile industry caused the increase of the heavy metal Zn in soil in the form of sludge. Waste treatment by the phytoremediation method is one of the alternatives of environmental refinement with low cost and high effectiveness. This study used sunflower plants (Helianthus annuus L.) as a phytoremediator. The chelating agent EDTA was added in some treatment to increase the heavy metal absorption. The addition of 1.5 kg sludge on growth media inhibits the sunflower growth. T0K0 treatment has the most efficient result with a total Zn absorption of 101.74 mg/kg. T0K0 treatment was able to reduce Zn up to 96%. In 100% soil treatment, the EDTA additions decreased the ability of sunflower plants to absorb Zn metal in the phytoremediation process. However, in the 40% sludge treatment, the addition of EDTA did not affect the ability of sunflower plants to absorb the Zn metal in the phytoremediation process.


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## Body

## 1. Introduction

Wastes are the major source of soil pollution and originate from mining, chemical, and metal-processing industries, textile industry, leather equality industry, and other allied industries [1, 2]. The development of the textile industry creates some negative impacts on the environment, one of which is the increase of the heavy metal Zn in the soil in the form of sludge [3–5]. The soil serves for providing nutrients, as a rooting medium, providing water, as a reservoir of water, and providing air for root respiration, as a place of cultivation of plants [4, 6, 7].Generally, zinc is an essential element which belongs to Group-II of the periodic table. It acts as a plant nutrient, but at higher concentrations, it is toxic [8]. Zinc is also a constituent of metalloenzyme or a cofactor for several enzymes such as anhydrases, dehydrogenases, oxidases, and peroxidases and plays an important role in regulating the nitrogen metabolism, cell multiplication, photosynthesis, and auxin synthesis in plants [9–12]. The function of zinc is to help a plant to produce chlorophyll. When the soil is deficient in zinc, leaves get discolored and plant growth is stunted [12–14]. Zinc deficiency causes leaf discoloration called chlorosis, a condition that causes tissue of the veins to turn yellow. Zinc toxicity also causes chlorosis in the younger leaves, which can extend to older leaves after prolonged exposure to high soil Zn levels [8, 15]. Zinc in excess reduces the germination, chlorophyll, carotenoid, sugar, amino acid, and growth of cluster beans (Cyamopsis tetragonoloba) [1].The toxicity of heavy metals is apparent in reducing growth and development of plants and seriously harms the health of animals and humans [2]. Increasing the concentration of the heavy metal Zn in the environment can cause serious effects on all life forms. For plants, heavy metal Zn pollution can cause shortening of the roots, leaf loss, chlorosis, and nutritional deficiencies. However, in pea (Pisum sativum), it reduces chlorophyll, photosynthesis, and plant growth [16, 17]. In rye grass (Lolium perenne), it reduces the growth, nutrient content, and photosynthetic energy conversion [18]. The acquired knowledge is to be used in planning of biomass processing systems and, in parallel, in phytoremediation of polluted soils, using cultivation of energy plants as a main concept [19, 20]. One technology that can be used to remediate the contaminated soil of heavy metals is phytoremediation [21, 22]. Phytoremediation is the use of plants for removal, stabilization, or destruction of pollutants in both organic and inorganic compounds [8, 15].The phytoremediation method has several advantages such as the method used is simple, efficient, cost-effective, and environmentally friendly [15]. In the phytoremediation process, sunflower plants (Helianthus annuus L.) are used as phytoremediators because these plants are hyperaccumulators and are fast growing plants with high biomass production [23, 24]. In addition to plant species, the phytoremediation process is also influenced by the presence of the chelating agent [25].The purpose of this research was to know the effect of adding a chelating agent to the absorption of sludge containing contaminated heavy metal by using sunflower plants (Helianthus annuus L.)


## 2. Methodology

### 2.1. Study Area

This research was conducted in Greenhouse, Integrated Laboratory, University of Brawijaya. This location is on the line 112°36′43″ East longitude and 7°54′58″ South latitude. This research was conducted in March 2017 until July 2017. Sludge of the textile industry was taken from PT. Behaestex, Pandaan, Pasuruan, Indonesia. Testing of the heavy metal content of Zn on soil and sunflower plant was tested using spectrophotometry at Chemical Laboratory, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, East Java, Indonesia.


### 2.2. Instruments

Some tools used in this research include pots as a place of planting media, sacks carrying planting medium, a jerigen container for sludge, shovels to plant crops, an analytical scale measuring plant mass, a soil tester to measure pH and temperature, a measuring cylinder to measure sludge volume, an oven for drying samples, gloves and masks as personal protective equipment, sample bottles as sample containers, stationery for pot information, a camera as a documentation tool, atomic absorption spectrophotometry (SSA) as a tool for testing heavy metal content of Zn, tubes reaction as a sample mixing container, a filter paper to filter the sample, an Erlenmeyer flask as a sample container, and a blender to smoothen the sample.


### 2.3. Materials

Materials used in this research include soil as planting medium, sludge as planting medium and treatment, sunflower plant as planting medium and treatment, compost as a provider of organic material for the plant and is useful to improve the soil structure, EDTA as the treatment material, water to watering plant, and ZnCl2 to add the Zn content in the treatment.


### 2.4. Research Stages

#### 2.4.1. Planting Medium

Prepare the pot as a container of planting media as many as 18 pieces. The prepared soil, sludge, and compost were each dried. Soil, sludge, and compost were then sieved with a 2 mm sieve. Each uniform soil, sludge, and compost was sampled for testing to determine the initial Zn, NPK, and water content in the material. The test was conducted to determine the required compost that is suitable for the needs of N and water. The soil and sludge that had been tested were mixed according to the treatment, soil without sludge and soil with 40% sludge. Then, it was homogenized by stirring and put into the pot that had been prepared as many as 18 pieces. The filled pots of each planting medium were weighed and calculated to know the field capacity.The availability of water in the soil was an important factor for the growth of the sunflower, and a good moisture content (% weight) of the sunflower clay texture was 62.18%. The capacity arrangement was done by taking samples of soil that are placed in a container that had two holes at the bottom and top, and then, the bottom hole was closed using a gauze that had been weighed previously. Furthermore, the initial soil was weighed to be 10 grams. Once weighed, the soil and the container were dipped into the water until filled sufficiently, and the top of the container hole was covered with plastic and then ignored until the soil is saturated in absorbing water. Then, the saturated soil was drained until no water is dripped and weighed as wet weight. Then, the soil was dried using an oven at 105°C for 24 hours and found dry soil. Herein, field capacity (FC) 100% can be obtained by the following calculation:(1)FC=wetweightofsoil−dryweightofsoildryweightofsoil×100.Addition of water was done to meet the needs of plants ranging from 60 to 100% of field capacity. In conjunction with water field capacity, 160 mg of ZnCl2 was added in 1 kg sludge by dissolving into water, and it was poured into sludge evenly with 3 times rinse and was left for 24 hours. Then, 2Na-EDTA chelating agent of 0 mg/kg, 876.72 mg/kg, and 1461.2 mg/kg was added to soil with three replications each. Before planting was done, the medium was added with compost as much as 3.3 gram, and then it was stirred until blended.


#### 2.4.2. Planting of Sunflower Seeds

Transplantation is the transfer of plants from a shedding medium to planting medium. In this study, seeds of sunflower plants were first taken on the tray of seedlings, and then, they entered the planting medium with each treatment. Planting on the tray is done for 1 week of plant age with an average height of 8 cm–10 cm and leaf number of 2–4 pieces.


#### 2.4.3. Monitoring

Maintenance of sunflower plants (Helianthus annuus L.) at the time of growth was done by watering the plants and also the selection of plants at the age of 2 weeks. The sunflower plants were watered with tap water with the provision of the water holding capacity of 60-100%, and rewatering was done if the water content was less than the water holding capacity. After 2 weeks of planting in pots, 2 sunflower plants would be selected for which the average growth had the same high characteristics and the same number of leaves for analysis. Provision of insecticides for 1-month-old plants is to prevent the arrival of pests.Observations of sunflower plants include measurements of room temperature and soil, soil pH, plant height, and leaf count calculations. Temperature and pH measurements were performed every day morning and evening using a soil tester. The measurement of plant height and leaf count calculation is done once every 1 week.


#### 2.4.4. Harvesting

Harvesting was done at the 8th week after planting. The samples were taken from 18 pots. Sunflower was taken the root, stems, and leaves to know the absorption of the heavy metal zinc (Zn) in sunflower plants. The stage of sunflower harvesting includes (1) separation of the stem from its roots by cutting the stem part above the soil surface, (2) separation of leaves from the stem, and (3) separation of roots with soil; after being separated, the roots are washed with water and then dried. The root of the leaf stalk is inserted into the sample place. The soil sample to be taken on one pot that was homogenized first was dried on the tray. The drying of the soil was carried out for 1 day. After drying, the soil was pounded using mortar. The soil that had been subtly ground was then put in a plastic clip of 300 grams. Then, samples of sunflower and soil had been taken to the laboratory for testing Zn concentration.


#### 2.4.5. Laboratory Test

Analysis of soil samples was performed twice, that is, on the initial soil and soil after treatment. Initial soil samples were taken for testing the concentrations of Zn and NPK metals contained in the initial soil. Soil after treatment only tested its Zn concentration. Testing of Zn metal concentration on soil was done at Chemical Laboratory, and NPK testing was conducted at Soil Chemistry Laboratory in University of Brawijaya.Testing of Zn concentration on soil begins by weighing 1 gram of the soil sample, it is then put into the reaction tube, and 10 mL of HNO3 is added. After it was heated for ±15 minutes with a temperature of 350°C, it was cooled. The quads were added to 25 mL and filtered with a 0.42 μ pore membrane filter. The tool used to read the concentration of the Zn metal on the soil was the Atomic Absorption Spectrophotometry (AAS) Shimadzu AA-6200 type.TotalN tests on the initial soil were carried out by the Kjeldahl method. The test procedure was to weigh 0.5 mm of 0.5 mm soil samples and then add 1 gram of selenium mixture and 5 mL of concentrated H2SO4, after which the mixed material was heated to 300°C. When the heating results were complete, the mixture was diluted with 50 mL pure H2O. The heating product was diluted to ±100 mL and added with 40% NaOH . 20 mL boric acid was added to the mixture; boric acid serves as an indicator of discoloration. The last step was to do the titration until the H2SO4 solution was at 0.1 N point.P test was done on soil using the spectrophotometric method, Bray-1 extract, and Bray-2 extract. The test procedure was to take 1.5 grams of soil samples that pass through a 2 mm sieve, add Bray and Kurtz I extract as much as 15 mL, shake it for 5 min, and filter with a membrane pore size 0.42μ. Then, add aquades up to 25 mL and phosphate reagent. Then, the result of the solution can be read on Spectronic 21.The testing procedure ofK concentration on the soil sample that was weighing 1 gram was added with 10 mL of the NH4OAc solution of pH 7 and homogenized. Then, it was filtered with a filter paper membrane with a pore size of 0.42 μ, and the filtrate accommodated in the container. Then, 10 mL of NH4OAc solution was added again, homogenized again, and filtered through a 0.42 μ pore membrane filter. Then, 10 mL of the NH4OAc solution was added again, the sample was centrifuged for 10 minutes, and the filtrate was collected back into the container. Calculations are performed using standard curves (standard curve regression).


#### 2.4.6. Data Analysis

This study used the Completely Randomized Design (CRD) method on 2 × 3 factorial with three repetitions. The first factor is the process of planting media, that are planting media without polluted sludge and planting media contaminated with 40% sludge. The second factor is the addition of EDTA concentration, that is, 0 mg/kg, 876.72 mg/kg, and 1461.2 mg/kg of soil. Each treatment consists of 3 repetitions.


## 2.1. Study Area

This research was conducted in Greenhouse, Integrated Laboratory, University of Brawijaya. This location is on the line 112°36′43″ East longitude and 7°54′58″ South latitude. This research was conducted in March 2017 until July 2017. Sludge of the textile industry was taken from PT. Behaestex, Pandaan, Pasuruan, Indonesia. Testing of the heavy metal content of Zn on soil and sunflower plant was tested using spectrophotometry at Chemical Laboratory, Faculty of Mathematics and Natural Sciences, University of Brawijaya, Malang, East Java, Indonesia.


## 2.2. Instruments

Some tools used in this research include pots as a place of planting media, sacks carrying planting medium, a jerigen container for sludge, shovels to plant crops, an analytical scale measuring plant mass, a soil tester to measure pH and temperature, a measuring cylinder to measure sludge volume, an oven for drying samples, gloves and masks as personal protective equipment, sample bottles as sample containers, stationery for pot information, a camera as a documentation tool, atomic absorption spectrophotometry (SSA) as a tool for testing heavy metal content of Zn, tubes reaction as a sample mixing container, a filter paper to filter the sample, an Erlenmeyer flask as a sample container, and a blender to smoothen the sample.


## 2.3. Materials

Materials used in this research include soil as planting medium, sludge as planting medium and treatment, sunflower plant as planting medium and treatment, compost as a provider of organic material for the plant and is useful to improve the soil structure, EDTA as the treatment material, water to watering plant, and ZnCl2 to add the Zn content in the treatment.


## 2.4. Research Stages

### 2.4.1. Planting Medium

Prepare the pot as a container of planting media as many as 18 pieces. The prepared soil, sludge, and compost were each dried. Soil, sludge, and compost were then sieved with a 2 mm sieve. Each uniform soil, sludge, and compost was sampled for testing to determine the initial Zn, NPK, and water content in the material. The test was conducted to determine the required compost that is suitable for the needs of N and water. The soil and sludge that had been tested were mixed according to the treatment, soil without sludge and soil with 40% sludge. Then, it was homogenized by stirring and put into the pot that had been prepared as many as 18 pieces. The filled pots of each planting medium were weighed and calculated to know the field capacity.The availability of water in the soil was an important factor for the growth of the sunflower, and a good moisture content (% weight) of the sunflower clay texture was 62.18%. The capacity arrangement was done by taking samples of soil that are placed in a container that had two holes at the bottom and top, and then, the bottom hole was closed using a gauze that had been weighed previously. Furthermore, the initial soil was weighed to be 10 grams. Once weighed, the soil and the container were dipped into the water until filled sufficiently, and the top of the container hole was covered with plastic and then ignored until the soil is saturated in absorbing water. Then, the saturated soil was drained until no water is dripped and weighed as wet weight. Then, the soil was dried using an oven at 105°C for 24 hours and found dry soil. Herein, field capacity (FC) 100% can be obtained by the following calculation:(1)FC=wetweightofsoil−dryweightofsoildryweightofsoil×100.Addition of water was done to meet the needs of plants ranging from 60 to 100% of field capacity. In conjunction with water field capacity, 160 mg of ZnCl2 was added in 1 kg sludge by dissolving into water, and it was poured into sludge evenly with 3 times rinse and was left for 24 hours. Then, 2Na-EDTA chelating agent of 0 mg/kg, 876.72 mg/kg, and 1461.2 mg/kg was added to soil with three replications each. Before planting was done, the medium was added with compost as much as 3.3 gram, and then it was stirred until blended.


### 2.4.2. Planting of Sunflower Seeds

Transplantation is the transfer of plants from a shedding medium to planting medium. In this study, seeds of sunflower plants were first taken on the tray of seedlings, and then, they entered the planting medium with each treatment. Planting on the tray is done for 1 week of plant age with an average height of 8 cm–10 cm and leaf number of 2–4 pieces.


### 2.4.3. Monitoring

Maintenance of sunflower plants (Helianthus annuus L.) at the time of growth was done by watering the plants and also the selection of plants at the age of 2 weeks. The sunflower plants were watered with tap water with the provision of the water holding capacity of 60-100%, and rewatering was done if the water content was less than the water holding capacity. After 2 weeks of planting in pots, 2 sunflower plants would be selected for which the average growth had the same high characteristics and the same number of leaves for analysis. Provision of insecticides for 1-month-old plants is to prevent the arrival of pests.Observations of sunflower plants include measurements of room temperature and soil, soil pH, plant height, and leaf count calculations. Temperature and pH measurements were performed every day morning and evening using a soil tester. The measurement of plant height and leaf count calculation is done once every 1 week.


### 2.4.4. Harvesting

Harvesting was done at the 8th week after planting. The samples were taken from 18 pots. Sunflower was taken the root, stems, and leaves to know the absorption of the heavy metal zinc (Zn) in sunflower plants. The stage of sunflower harvesting includes (1) separation of the stem from its roots by cutting the stem part above the soil surface, (2) separation of leaves from the stem, and (3) separation of roots with soil; after being separated, the roots are washed with water and then dried. The root of the leaf stalk is inserted into the sample place. The soil sample to be taken on one pot that was homogenized first was dried on the tray. The drying of the soil was carried out for 1 day. After drying, the soil was pounded using mortar. The soil that had been subtly ground was then put in a plastic clip of 300 grams. Then, samples of sunflower and soil had been taken to the laboratory for testing Zn concentration.


### 2.4.5. Laboratory Test

Analysis of soil samples was performed twice, that is, on the initial soil and soil after treatment. Initial soil samples were taken for testing the concentrations of Zn and NPK metals contained in the initial soil. Soil after treatment only tested its Zn concentration. Testing of Zn metal concentration on soil was done at Chemical Laboratory, and NPK testing was conducted at Soil Chemistry Laboratory in University of Brawijaya.Testing of Zn concentration on soil begins by weighing 1 gram of the soil sample, it is then put into the reaction tube, and 10 mL of HNO3 is added. After it was heated for ±15 minutes with a temperature of 350°C, it was cooled. The quads were added to 25 mL and filtered with a 0.42 μ pore membrane filter. The tool used to read the concentration of the Zn metal on the soil was the Atomic Absorption Spectrophotometry (AAS) Shimadzu AA-6200 type.TotalN tests on the initial soil were carried out by the Kjeldahl method. The test procedure was to weigh 0.5 mm of 0.5 mm soil samples and then add 1 gram of selenium mixture and 5 mL of concentrated H2SO4, after which the mixed material was heated to 300°C. When the heating results were complete, the mixture was diluted with 50 mL pure H2O. The heating product was diluted to ±100 mL and added with 40% NaOH . 20 mL boric acid was added to the mixture; boric acid serves as an indicator of discoloration. The last step was to do the titration until the H2SO4 solution was at 0.1 N point.P test was done on soil using the spectrophotometric method, Bray-1 extract, and Bray-2 extract. The test procedure was to take 1.5 grams of soil samples that pass through a 2 mm sieve, add Bray and Kurtz I extract as much as 15 mL, shake it for 5 min, and filter with a membrane pore size 0.42μ. Then, add aquades up to 25 mL and phosphate reagent. Then, the result of the solution can be read on Spectronic 21.The testing procedure ofK concentration on the soil sample that was weighing 1 gram was added with 10 mL of the NH4OAc solution of pH 7 and homogenized. Then, it was filtered with a filter paper membrane with a pore size of 0.42 μ, and the filtrate accommodated in the container. Then, 10 mL of NH4OAc solution was added again, homogenized again, and filtered through a 0.42 μ pore membrane filter. Then, 10 mL of the NH4OAc solution was added again, the sample was centrifuged for 10 minutes, and the filtrate was collected back into the container. Calculations are performed using standard curves (standard curve regression).


### 2.4.6. Data Analysis

This study used the Completely Randomized Design (CRD) method on 2 × 3 factorial with three repetitions. The first factor is the process of planting media, that are planting media without polluted sludge and planting media contaminated with 40% sludge. The second factor is the addition of EDTA concentration, that is, 0 mg/kg, 876.72 mg/kg, and 1461.2 mg/kg of soil. Each treatment consists of 3 repetitions.


## 2.4.1. Planting Medium

Prepare the pot as a container of planting media as many as 18 pieces. The prepared soil, sludge, and compost were each dried. Soil, sludge, and compost were then sieved with a 2 mm sieve. Each uniform soil, sludge, and compost was sampled for testing to determine the initial Zn, NPK, and water content in the material. The test was conducted to determine the required compost that is suitable for the needs of N and water. The soil and sludge that had been tested were mixed according to the treatment, soil without sludge and soil with 40% sludge. Then, it was homogenized by stirring and put into the pot that had been prepared as many as 18 pieces. The filled pots of each planting medium were weighed and calculated to know the field capacity.The availability of water in the soil was an important factor for the growth of the sunflower, and a good moisture content (% weight) of the sunflower clay texture was 62.18%. The capacity arrangement was done by taking samples of soil that are placed in a container that had two holes at the bottom and top, and then, the bottom hole was closed using a gauze that had been weighed previously. Furthermore, the initial soil was weighed to be 10 grams. Once weighed, the soil and the container were dipped into the water until filled sufficiently, and the top of the container hole was covered with plastic and then ignored until the soil is saturated in absorbing water. Then, the saturated soil was drained until no water is dripped and weighed as wet weight. Then, the soil was dried using an oven at 105°C for 24 hours and found dry soil. Herein, field capacity (FC) 100% can be obtained by the following calculation:(1)FC=wetweightofsoil−dryweightofsoildryweightofsoil×100.Addition of water was done to meet the needs of plants ranging from 60 to 100% of field capacity. In conjunction with water field capacity, 160 mg of ZnCl2 was added in 1 kg sludge by dissolving into water, and it was poured into sludge evenly with 3 times rinse and was left for 24 hours. Then, 2Na-EDTA chelating agent of 0 mg/kg, 876.72 mg/kg, and 1461.2 mg/kg was added to soil with three replications each. Before planting was done, the medium was added with compost as much as 3.3 gram, and then it was stirred until blended.


## 2.4.2. Planting of Sunflower Seeds

Transplantation is the transfer of plants from a shedding medium to planting medium. In this study, seeds of sunflower plants were first taken on the tray of seedlings, and then, they entered the planting medium with each treatment. Planting on the tray is done for 1 week of plant age with an average height of 8 cm–10 cm and leaf number of 2–4 pieces.


## 2.4.3. Monitoring

Maintenance of sunflower plants (Helianthus annuus L.) at the time of growth was done by watering the plants and also the selection of plants at the age of 2 weeks. The sunflower plants were watered with tap water with the provision of the water holding capacity of 60-100%, and rewatering was done if the water content was less than the water holding capacity. After 2 weeks of planting in pots, 2 sunflower plants would be selected for which the average growth had the same high characteristics and the same number of leaves for analysis. Provision of insecticides for 1-month-old plants is to prevent the arrival of pests.Observations of sunflower plants include measurements of room temperature and soil, soil pH, plant height, and leaf count calculations. Temperature and pH measurements were performed every day morning and evening using a soil tester. The measurement of plant height and leaf count calculation is done once every 1 week.


## 2.4.4. Harvesting

Harvesting was done at the 8th week after planting. The samples were taken from 18 pots. Sunflower was taken the root, stems, and leaves to know the absorption of the heavy metal zinc (Zn) in sunflower plants. The stage of sunflower harvesting includes (1) separation of the stem from its roots by cutting the stem part above the soil surface, (2) separation of leaves from the stem, and (3) separation of roots with soil; after being separated, the roots are washed with water and then dried. The root of the leaf stalk is inserted into the sample place. The soil sample to be taken on one pot that was homogenized first was dried on the tray. The drying of the soil was carried out for 1 day. After drying, the soil was pounded using mortar. The soil that had been subtly ground was then put in a plastic clip of 300 grams. Then, samples of sunflower and soil had been taken to the laboratory for testing Zn concentration.


## 2.4.5. Laboratory Test

Analysis of soil samples was performed twice, that is, on the initial soil and soil after treatment. Initial soil samples were taken for testing the concentrations of Zn and NPK metals contained in the initial soil. Soil after treatment only tested its Zn concentration. Testing of Zn metal concentration on soil was done at Chemical Laboratory, and NPK testing was conducted at Soil Chemistry Laboratory in University of Brawijaya.Testing of Zn concentration on soil begins by weighing 1 gram of the soil sample, it is then put into the reaction tube, and 10 mL of HNO3 is added. After it was heated for ±15 minutes with a temperature of 350°C, it was cooled. The quads were added to 25 mL and filtered with a 0.42 μ pore membrane filter. The tool used to read the concentration of the Zn metal on the soil was the Atomic Absorption Spectrophotometry (AAS) Shimadzu AA-6200 type.TotalN tests on the initial soil were carried out by the Kjeldahl method. The test procedure was to weigh 0.5 mm of 0.5 mm soil samples and then add 1 gram of selenium mixture and 5 mL of concentrated H2SO4, after which the mixed material was heated to 300°C. When the heating results were complete, the mixture was diluted with 50 mL pure H2O. The heating product was diluted to ±100 mL and added with 40% NaOH . 20 mL boric acid was added to the mixture; boric acid serves as an indicator of discoloration. The last step was to do the titration until the H2SO4 solution was at 0.1 N point.P test was done on soil using the spectrophotometric method, Bray-1 extract, and Bray-2 extract. The test procedure was to take 1.5 grams of soil samples that pass through a 2 mm sieve, add Bray and Kurtz I extract as much as 15 mL, shake it for 5 min, and filter with a membrane pore size 0.42μ. Then, add aquades up to 25 mL and phosphate reagent. Then, the result of the solution can be read on Spectronic 21.The testing procedure ofK concentration on the soil sample that was weighing 1 gram was added with 10 mL of the NH4OAc solution of pH 7 and homogenized. Then, it was filtered with a filter paper membrane with a pore size of 0.42 μ, and the filtrate accommodated in the container. Then, 10 mL of NH4OAc solution was added again, homogenized again, and filtered through a 0.42 μ pore membrane filter. Then, 10 mL of the NH4OAc solution was added again, the sample was centrifuged for 10 minutes, and the filtrate was collected back into the container. Calculations are performed using standard curves (standard curve regression).


## 2.4.6. Data Analysis

This study used the Completely Randomized Design (CRD) method on 2 × 3 factorial with three repetitions. The first factor is the process of planting media, that are planting media without polluted sludge and planting media contaminated with 40% sludge. The second factor is the addition of EDTA concentration, that is, 0 mg/kg, 876.72 mg/kg, and 1461.2 mg/kg of soil. Each treatment consists of 3 repetitions.


## 3. Results and Discussion

### 3.1. Characteristics of Planting Media and Materials Prior to Treatment

Physical characteristics of the soil before the research were solid form, black, and odorless. The physical characteristics of sludge were solid, brown, and smelly. The physical characteristics of compost were solid, black, and odorless. Each planting medium was tested early to determine the Zn levels used before. The testing results of the initial Zn content of planting medium can be seen in Table1.Table 1
Zn test results on planting media.
Number
Media
Analysis results
Zn levels
Units
1
Soil
186.77 ± 0.12
mg/kg
2
Sludge
0.13 ± 0.00
%
3
Compost
0.02 ± 0.00
%
4
Faucet water
Not detected
mg/L


### 3.2. Environmental Condition during Research

Environmental conditions during the study include room temperature and soil temperature. The room was measured, and soil temperature was noted to know the condition of temperature in place of research (greenhouse) and also on soil planting media. Room temperature measurements were performed at the minimum temperature (morning) from 07:00 to 08:00 and at the maximum temperature (noon) from 12:00 to 13:00 daily for 42 days. The average graph of room temperature of the study site and the average soil temperature can be seen in Figure1.Figure 1
Graph of temperature: (a) room temperature and (b) soil temperature.
(a)
(b)Figure1(a) shows the temperature of the greenhouse room during sunflower plant growth with a mean value of 37°C and an average temperature of at least 21°C. This is in accordance with the opinion of Khalifa et al.[23], where the maximum growth of indoor sunflower germination occurs at a temperature of 20.4°C to 37.6°C.Figure1(b) shows the soil temperature during the growth of sunflower plants. It also shows that it still meets the tolerable temperature tolerance limits with a mean value of a maximum temperature of 35.17°C and a minimum average temperature of 21.33°C. The growth of sprouts and sunflower seeds (Helianthus annuus L.) is good at temperatures of 10°C and 35.5°C.Figure2 shows that the average soil pH per week in the T1 treatment (60% soil + 40% sludge) is higher than in the T0 treatment (100% soil). The T1 treatment had an average soil pH ranging from 6.8 to 7.0, whereas the T0 treatment had an average soil pH ranging from 6.0 to 6.8. Sunflower plants grow well at a pH ranging from 5.7 to 8.1. The soil pH reaction outside the 6-7 range may result in a decrease in the availability of certain nutrients and sometimes lead to the availability of other nutrients.Figure 2
Average graph of soil pH.


### 3.3. Plant Height

The height of sunflower plants at the beginning of planting had the same average height of 8 cm. At the 1st WAT (weeks after transplant), the high growth of sunflower plants tended to have no significant difference. The height of the sunflower plants in the T0K0 and T1K0 treatments (without EDTA addition) showed significantly different growth at 2 weeks after transplantation. The 3rd week after transplantation of each treatment tends to show the difference of plant growth that is real but still related. Plant height growth at T0K0 was significantly higher than that in other treatments at the 4th–8th WAT. Treatment of T0K1, T0K2, T1K1, and T1K2 with EDTA addition had significantly different plant height growth that is still related. Treatment without EDTA addition tended to have higher high growth compared with treatment with EDTA addition.T0 treatment had higher plant growth than T1 treatment, and this was because the addition of sludge on planting medium causes plant growth to be inhibited. Sludge had a hard characteristic that makes it difficult for rooting zones to grow [6]. The increasing concentration of EDTA also causes plant growth to be inhibited. The addition of EDTA to the growing medium causes Zn available in the soil which is then absorbed by the increasing sunflower plants. The average graph of high growth of sunflower can be seen in Figure 3.Figure 3
Average growth of plants.Growth of sunflower plants had increased weekly (1st–8th WAT). Differences in the plant height began to be seen in the 4th WAT. At the 4th–8th WAT, the T0K0 treatment showed a significant high difference compared with other treatments.The T0K0 treatment had the best high growth compared to other treatments. T0 treatment (100% soil) with the addition of different EDTA concentrations had a significant high difference at the 8th WAT. Treatment of T1 (60% soil + 40% sludge) with the addition of different EDTA concentrations tended not to have a significant high difference at the 8th WAT. Maximum height growth of sunflower plants is found in the T0K0 treatment, and the minimal growth of sunflower plants is found in the T1K2 treatment. The addition of sludge to planting media not only damages the root zone due to its hard texture but also inhibits the growth of sunflower plants due to its high Zn content. The increasing concentration of EDTA also causes the heavy metal content of Zn in soil to increase. According to Novitasari et al. [24], based on observation data on the effect of metal Zn concentration on the plant height taken for 8 weeks, the higher Zn concentration would reduce the height of the sunflower plants.T0 treatment had an average leaf number greater than T1 treatment. The average grain growth of leaf number indicates that the addition of sludge with higher Zn concentration causes stunted leaf growth. The graph of average growth of leaf amount can be seen in Figure4.Figure 4
Graph of the average growth of leaves amount.The excess Zn metal inJatropha plants causes yellowing of leaves. When the plants are exposed to excess metal Zn for longer time, the yellowish leaves turn brown, showing the symptoms of chlorosis and necrosis, and become small. Based on the research Novitasari [24], the higher Zn content in plants will cause more severe leaf damage such as chlorosis, necrosis, and abnormal leaf growth. Leaf damage can also be caused by environmental conditions such as animal/pest disturbance at the time of the research.


### 3.4. Wet Weight and Dry Weight Plants

Wet weight and dry weight were measured at the 9th week to determine the mass growth of sunflower plants. Measurement of wet weight and dry weight is divided into 4 parts: flowers, leaf, trunk, and root. Data on the measurement of wet weight and dry weight of sunflower plants at the 8th WAT can be seen in Tables2 and 3, respectively.Table 2
Wet weight of plants.
Treatment
Root
Trunk
Flower
Leaf
Total
g/plant
T0K0
4.88b
13.93c
17.76d
6.60c
31.33c
T0K1
1.94a
8.01b
11.76c
3.91b
17.79b
T0K2
2.00a
5.46ab
11.75c
3.36b
14.74b
T1K0
0.81a
3.27a
5.55b
1.11ab
7.05ab
T1K1
0.56a
1.80a
2.77a
0.57a
3.87a
T1K2
0.78a
2.12a
3.53ab
0.71a
4.80a
BNT 5%
1.82
4.68
2.76
2.28
9.46
The numbers followed by different letters in the same column give different effect to the height of sunflower plants (Helianthus annuus L.) at 5% LSD (least significant difference) test level.Table 3
Dry weight of plants.
Treatment
Root
Trunk
Flower
Leaf
Total
g/plant
T0K0
0.76b
2.07c
0.98bc
1.25c
5.06c
T0K1
0.21a
1.08bc
0.62b
0.70b
2.62b
T0K2
0.18a
0.91b
0.64b
0.67b
2.42b
T1K0
0.12a
0.62ab
0.34ab
0.30a
1.40ab
T1K1
0.07a
0.29a
0.17a
0.16a
0.69a
T1K2
0.12a
0.40ab
0.23ab
0.21a
0.96a
BNT 5%
0.40
0.59
0.44
0.59
1.34
The numbers followed by different letters in the same column give different effect to the height of sunflower plants (Helianthus annuus L.) at 5% LSD (least significant difference) test level.The wet weight of roots, leaves, flowers, and stems was significantly different at 5% significance level. The T0K0 treatment had a significantly higher wet weight value compared to the other treatments at the root.In the treated roots, T0K0 and T0K1 had significantly higher wet weight values compared to other treatments. T0 treatment had shown significant differences in wet weight on the flower portion compared with T1 treatment.Statistical calculations of dry weight show that the root, leaf, flower, and stem had different effects on the 5% confidence level. The T0K0 treatment had a significantly higher dry weight compared with the other treatments at the root. The stem section shows that T0 treatment had a significantly higher dry weight than T1 treatment. The interest rate tends to show no significant difference in dry weight in each treatment. The T0 treatment had a significantly higher dry weight than the T1 treatment on the leaf. T0 treatment tended to have higher dry weight than T1 treatment for the total dry weight of sunflower plants.In both wet weight and dry weight, T0 treatment (100% soil) has an average weight of plants higher than T1 treatment (60% soil + 40% sludge). Sunflower plants grown on planting media with a combination of sludge growth is inhibited.This is because the planting medium with a combination of sludge contains higher levels of Zn compared with soil crop media sludge characteristics that tend to be hard and dry, which also make the development of the root zone of sunflower plants to be disturbed. The result is that sunflower plants grown on planting medium with a combination of sludge has a smaller growth rate compared to that grown on soil media. In accordance with the opinion of Mcbride et al. [8], the addition of sludge to the soil resulted in increased permeability on the soil. Permeability affects root penetration, water penetration rate, water absorption rate, internal drainage, and nutrient leaching [3]. According to Norvell [5], the results observed from the growth of sengon and mangium seedlings in solid soil suggest that the depth of root penetration and high growth decreases with increasing soil density.The results of wet weight and dry weight tests also show that the increasing concentration of EDTA in planting medium inhibits higher growth of sunflower plants. The increasing concentration of EDTA in the soil caused the high concentration of the heavy metal Zn on the ground, which was then absorbed by the tissue of sunflower plants (Helianthus annuus L.)


### 3.5. Analysis of Zn Concentration in Soil and Plants

This study analyzes the concentration of the heavy metal Zn found in soil and sunflower plants to determine the concentration of Zn in soil and plants. The plant part consists of roots, leaves, flowers, and stems. The effect of sludge and EDTA addition treatment on the mean of total Zn concentration on the soil and sunflower plants can be seen in Table4.Table 4
Level of Zn metal on soil and sunflower plants.
Treatment
Concentration of Zn
Uptake of Zn (mg/plant)
Soil (mg/kg)
Plant (mg/kg)
T0K0
163.62a
101.74b
179.00c
T0K1
159.8a
136.91bc
122.33b
T0K2
155.57a
160.17c
139.67bc
T1K0
289.31c
61.22a
31.33a
T1K1
251.07b
151.71c
38.67a
T1K2
306.31c
137.35bc
53.33ab
BNT 5%
21.37
37.79
71.23
The numbers followed by different letters in the same column give different effect to the height of sunflower plants (Helianthus annuus L.) at 5% LSD (least significant difference) test level.The average concentration of Zn on soil and plants has a significant difference in the 5% confidence level. The accumulation of the Zn metal on soil showed significant differences between T0 and T1 treatments.The value of Zn metal content is highest in T1K2 treatment and lowest in T0K2 treatment. The result of the Zn test on the plant showed significant differences, and the value of the Zn metal content is highest in T0K2 treatment and lowest in T1K0 treatment.The uptake value obtained from the dry weight of the plant divided by the number of plants per pot was then multiplied by the concentration of Zn. T0 treatment (100% soil) had a higher uptake value compared with T1 treatment (60% soil + 40% sludge).T1 treatment had a higher Zn concentration on soil compared with T0 treatment. This was because sludge had a higher initial Zn content than the soil. The addition of EDTA had an effect on the Zn concentration in plants. The more the EDTA addition, the higher the Zn concentration in the plant. Uptake values tend not to have significant differences, and the highest uptake value in the T0K0 treatment was with a total absorption of 179.00 mg/plant.Zn concentration which was absorbed by sunflower plants (Helianthus annuus L.) was Zn available in soil. The addition of chelating compounds in the soil may not only increase the total concentration of dissolved metals but also alter the absorption mechanism. Depending on the type of the metal, the type of the plant, and the concentration of the engaging compound, absorption is significantly improved [12].The removal percentage was calculated to determine the absorption ability of sunflower plants in reducing the heavy metal Zn. The efficiency of heavy metal removal by plants is determined by(2)efficiency=ZnuptakeinitialZn,Table5 shows that T0 treatment with an initial Zn content of soil of 186.77 mg/kg can reduce Zn weight by 96% (T0K0), 65% (T0K1), and 75% (T0K2). T1 treatment with an initial Zn content in soil of 696,062 mg/kg was capable of reducing Zn weight by 4% (T1K0), 6% (T1K1), and 8% (T1K2).Table 5
Efficiency of Zn weight loss by sunflower plants.
Treatment
Percentage removal
T0K0
96
T0K1
65
T0K2
75
T1K0
4
T1K1
6
T1K2
8T0 treatment (100% soil) showed better removal efficiency compared to T1 (60% soil + 40% sludge). This was because T1 treatment had a very high initial Zn content compared with T0 treatment. The very high content of the heavy metal Zn on the soil causes toxic effects on plants that can decrease the ability of plant absorption in the phytoremediation process. According to Namgay et al. [12], plant growth requires only a small amount of Zn content compared to macronutrients. Zn in large quantities would cause damage to plant tissue.The addition of EDTA causes the growth of sunflower plants to be inhibited and can decrease the absorption efficiency of the heavy metal Zn by sunflower plants. The addition of EDTA to the T1 treatment tended to have no significant effect. This is because the initial Zn content of the T1 treatment is too high, causing the sunflower plants to have heavy metal Zn toxicity and damage to the plant tissue.


## 3.1. Characteristics of Planting Media and Materials Prior to Treatment

Physical characteristics of the soil before the research were solid form, black, and odorless. The physical characteristics of sludge were solid, brown, and smelly. The physical characteristics of compost were solid, black, and odorless. Each planting medium was tested early to determine the Zn levels used before. The testing results of the initial Zn content of planting medium can be seen in Table1.Table 1
Zn test results on planting media.
Number
Media
Analysis results
Zn levels
Units
1
Soil
186.77 ± 0.12
mg/kg
2
Sludge
0.13 ± 0.00
%
3
Compost
0.02 ± 0.00
%
4
Faucet water
Not detected
mg/L


## 3.2. Environmental Condition during Research

Environmental conditions during the study include room temperature and soil temperature. The room was measured, and soil temperature was noted to know the condition of temperature in place of research (greenhouse) and also on soil planting media. Room temperature measurements were performed at the minimum temperature (morning) from 07:00 to 08:00 and at the maximum temperature (noon) from 12:00 to 13:00 daily for 42 days. The average graph of room temperature of the study site and the average soil temperature can be seen in Figure1.Figure 1
Graph of temperature: (a) room temperature and (b) soil temperature.
(a)
(b)Figure1(a) shows the temperature of the greenhouse room during sunflower plant growth with a mean value of 37°C and an average temperature of at least 21°C. This is in accordance with the opinion of Khalifa et al.[23], where the maximum growth of indoor sunflower germination occurs at a temperature of 20.4°C to 37.6°C.Figure1(b) shows the soil temperature during the growth of sunflower plants. It also shows that it still meets the tolerable temperature tolerance limits with a mean value of a maximum temperature of 35.17°C and a minimum average temperature of 21.33°C. The growth of sprouts and sunflower seeds (Helianthus annuus L.) is good at temperatures of 10°C and 35.5°C.Figure2 shows that the average soil pH per week in the T1 treatment (60% soil + 40% sludge) is higher than in the T0 treatment (100% soil). The T1 treatment had an average soil pH ranging from 6.8 to 7.0, whereas the T0 treatment had an average soil pH ranging from 6.0 to 6.8. Sunflower plants grow well at a pH ranging from 5.7 to 8.1. The soil pH reaction outside the 6-7 range may result in a decrease in the availability of certain nutrients and sometimes lead to the availability of other nutrients.Figure 2
Average graph of soil pH.


## 3.3. Plant Height

The height of sunflower plants at the beginning of planting had the same average height of 8 cm. At the 1st WAT (weeks after transplant), the high growth of sunflower plants tended to have no significant difference. The height of the sunflower plants in the T0K0 and T1K0 treatments (without EDTA addition) showed significantly different growth at 2 weeks after transplantation. The 3rd week after transplantation of each treatment tends to show the difference of plant growth that is real but still related. Plant height growth at T0K0 was significantly higher than that in other treatments at the 4th–8th WAT. Treatment of T0K1, T0K2, T1K1, and T1K2 with EDTA addition had significantly different plant height growth that is still related. Treatment without EDTA addition tended to have higher high growth compared with treatment with EDTA addition.T0 treatment had higher plant growth than T1 treatment, and this was because the addition of sludge on planting medium causes plant growth to be inhibited. Sludge had a hard characteristic that makes it difficult for rooting zones to grow [6]. The increasing concentration of EDTA also causes plant growth to be inhibited. The addition of EDTA to the growing medium causes Zn available in the soil which is then absorbed by the increasing sunflower plants. The average graph of high growth of sunflower can be seen in Figure 3.Figure 3
Average growth of plants.Growth of sunflower plants had increased weekly (1st–8th WAT). Differences in the plant height began to be seen in the 4th WAT. At the 4th–8th WAT, the T0K0 treatment showed a significant high difference compared with other treatments.The T0K0 treatment had the best high growth compared to other treatments. T0 treatment (100% soil) with the addition of different EDTA concentrations had a significant high difference at the 8th WAT. Treatment of T1 (60% soil + 40% sludge) with the addition of different EDTA concentrations tended not to have a significant high difference at the 8th WAT. Maximum height growth of sunflower plants is found in the T0K0 treatment, and the minimal growth of sunflower plants is found in the T1K2 treatment. The addition of sludge to planting media not only damages the root zone due to its hard texture but also inhibits the growth of sunflower plants due to its high Zn content. The increasing concentration of EDTA also causes the heavy metal content of Zn in soil to increase. According to Novitasari et al. [24], based on observation data on the effect of metal Zn concentration on the plant height taken for 8 weeks, the higher Zn concentration would reduce the height of the sunflower plants.T0 treatment had an average leaf number greater than T1 treatment. The average grain growth of leaf number indicates that the addition of sludge with higher Zn concentration causes stunted leaf growth. The graph of average growth of leaf amount can be seen in Figure4.Figure 4
Graph of the average growth of leaves amount.The excess Zn metal inJatropha plants causes yellowing of leaves. When the plants are exposed to excess metal Zn for longer time, the yellowish leaves turn brown, showing the symptoms of chlorosis and necrosis, and become small. Based on the research Novitasari [24], the higher Zn content in plants will cause more severe leaf damage such as chlorosis, necrosis, and abnormal leaf growth. Leaf damage can also be caused by environmental conditions such as animal/pest disturbance at the time of the research.


## 3.4. Wet Weight and Dry Weight Plants

Wet weight and dry weight were measured at the 9th week to determine the mass growth of sunflower plants. Measurement of wet weight and dry weight is divided into 4 parts: flowers, leaf, trunk, and root. Data on the measurement of wet weight and dry weight of sunflower plants at the 8th WAT can be seen in Tables2 and 3, respectively.Table 2
Wet weight of plants.
Treatment
Root
Trunk
Flower
Leaf
Total
g/plant
T0K0
4.88b
13.93c
17.76d
6.60c
31.33c
T0K1
1.94a
8.01b
11.76c
3.91b
17.79b
T0K2
2.00a
5.46ab
11.75c
3.36b
14.74b
T1K0
0.81a
3.27a
5.55b
1.11ab
7.05ab
T1K1
0.56a
1.80a
2.77a
0.57a
3.87a
T1K2
0.78a
2.12a
3.53ab
0.71a
4.80a
BNT 5%
1.82
4.68
2.76
2.28
9.46
The numbers followed by different letters in the same column give different effect to the height of sunflower plants (Helianthus annuus L.) at 5% LSD (least significant difference) test level.Table 3
Dry weight of plants.
Treatment
Root
Trunk
Flower
Leaf
Total
g/plant
T0K0
0.76b
2.07c
0.98bc
1.25c
5.06c
T0K1
0.21a
1.08bc
0.62b
0.70b
2.62b
T0K2
0.18a
0.91b
0.64b
0.67b
2.42b
T1K0
0.12a
0.62ab
0.34ab
0.30a
1.40ab
T1K1
0.07a
0.29a
0.17a
0.16a
0.69a
T1K2
0.12a
0.40ab
0.23ab
0.21a
0.96a
BNT 5%
0.40
0.59
0.44
0.59
1.34
The numbers followed by different letters in the same column give different effect to the height of sunflower plants (Helianthus annuus L.) at 5% LSD (least significant difference) test level.The wet weight of roots, leaves, flowers, and stems was significantly different at 5% significance level. The T0K0 treatment had a significantly higher wet weight value compared to the other treatments at the root.In the treated roots, T0K0 and T0K1 had significantly higher wet weight values compared to other treatments. T0 treatment had shown significant differences in wet weight on the flower portion compared with T1 treatment.Statistical calculations of dry weight show that the root, leaf, flower, and stem had different effects on the 5% confidence level. The T0K0 treatment had a significantly higher dry weight compared with the other treatments at the root. The stem section shows that T0 treatment had a significantly higher dry weight than T1 treatment. The interest rate tends to show no significant difference in dry weight in each treatment. The T0 treatment had a significantly higher dry weight than the T1 treatment on the leaf. T0 treatment tended to have higher dry weight than T1 treatment for the total dry weight of sunflower plants.In both wet weight and dry weight, T0 treatment (100% soil) has an average weight of plants higher than T1 treatment (60% soil + 40% sludge). Sunflower plants grown on planting media with a combination of sludge growth is inhibited.This is because the planting medium with a combination of sludge contains higher levels of Zn compared with soil crop media sludge characteristics that tend to be hard and dry, which also make the development of the root zone of sunflower plants to be disturbed. The result is that sunflower plants grown on planting medium with a combination of sludge has a smaller growth rate compared to that grown on soil media. In accordance with the opinion of Mcbride et al. [8], the addition of sludge to the soil resulted in increased permeability on the soil. Permeability affects root penetration, water penetration rate, water absorption rate, internal drainage, and nutrient leaching [3]. According to Norvell [5], the results observed from the growth of sengon and mangium seedlings in solid soil suggest that the depth of root penetration and high growth decreases with increasing soil density.The results of wet weight and dry weight tests also show that the increasing concentration of EDTA in planting medium inhibits higher growth of sunflower plants. The increasing concentration of EDTA in the soil caused the high concentration of the heavy metal Zn on the ground, which was then absorbed by the tissue of sunflower plants (Helianthus annuus L.)


## 3.5. Analysis of Zn Concentration in Soil and Plants

This study analyzes the concentration of the heavy metal Zn found in soil and sunflower plants to determine the concentration of Zn in soil and plants. The plant part consists of roots, leaves, flowers, and stems. The effect of sludge and EDTA addition treatment on the mean of total Zn concentration on the soil and sunflower plants can be seen in Table4.Table 4
Level of Zn metal on soil and sunflower plants.
Treatment
Concentration of Zn
Uptake of Zn (mg/plant)
Soil (mg/kg)
Plant (mg/kg)
T0K0
163.62a
101.74b
179.00c
T0K1
159.8a
136.91bc
122.33b
T0K2
155.57a
160.17c
139.67bc
T1K0
289.31c
61.22a
31.33a
T1K1
251.07b
151.71c
38.67a
T1K2
306.31c
137.35bc
53.33ab
BNT 5%
21.37
37.79
71.23
The numbers followed by different letters in the same column give different effect to the height of sunflower plants (Helianthus annuus L.) at 5% LSD (least significant difference) test level.The average concentration of Zn on soil and plants has a significant difference in the 5% confidence level. The accumulation of the Zn metal on soil showed significant differences between T0 and T1 treatments.The value of Zn metal content is highest in T1K2 treatment and lowest in T0K2 treatment. The result of the Zn test on the plant showed significant differences, and the value of the Zn metal content is highest in T0K2 treatment and lowest in T1K0 treatment.The uptake value obtained from the dry weight of the plant divided by the number of plants per pot was then multiplied by the concentration of Zn. T0 treatment (100% soil) had a higher uptake value compared with T1 treatment (60% soil + 40% sludge).T1 treatment had a higher Zn concentration on soil compared with T0 treatment. This was because sludge had a higher initial Zn content than the soil. The addition of EDTA had an effect on the Zn concentration in plants. The more the EDTA addition, the higher the Zn concentration in the plant. Uptake values tend not to have significant differences, and the highest uptake value in the T0K0 treatment was with a total absorption of 179.00 mg/plant.Zn concentration which was absorbed by sunflower plants (Helianthus annuus L.) was Zn available in soil. The addition of chelating compounds in the soil may not only increase the total concentration of dissolved metals but also alter the absorption mechanism. Depending on the type of the metal, the type of the plant, and the concentration of the engaging compound, absorption is significantly improved [12].The removal percentage was calculated to determine the absorption ability of sunflower plants in reducing the heavy metal Zn. The efficiency of heavy metal removal by plants is determined by(2)efficiency=ZnuptakeinitialZn,Table5 shows that T0 treatment with an initial Zn content of soil of 186.77 mg/kg can reduce Zn weight by 96% (T0K0), 65% (T0K1), and 75% (T0K2). T1 treatment with an initial Zn content in soil of 696,062 mg/kg was capable of reducing Zn weight by 4% (T1K0), 6% (T1K1), and 8% (T1K2).Table 5
Efficiency of Zn weight loss by sunflower plants.
Treatment
Percentage removal
T0K0
96
T0K1
65
T0K2
75
T1K0
4
T1K1
6
T1K2
8T0 treatment (100% soil) showed better removal efficiency compared to T1 (60% soil + 40% sludge). This was because T1 treatment had a very high initial Zn content compared with T0 treatment. The very high content of the heavy metal Zn on the soil causes toxic effects on plants that can decrease the ability of plant absorption in the phytoremediation process. According to Namgay et al. [12], plant growth requires only a small amount of Zn content compared to macronutrients. Zn in large quantities would cause damage to plant tissue.The addition of EDTA causes the growth of sunflower plants to be inhibited and can decrease the absorption efficiency of the heavy metal Zn by sunflower plants. The addition of EDTA to the T1 treatment tended to have no significant effect. This is because the initial Zn content of the T1 treatment is too high, causing the sunflower plants to have heavy metal Zn toxicity and damage to the plant tissue.


## 4. Conclusion

(1)
The sunflower planted in the T1 treatment (60% soil + 40% sludge) has lower mean height and weight than the sunflower planted in the T0 treatment (100% soil). The addition of sludge as much as 1.5 kg on planting medium resulted in the inhibited growth of sunflower plants (Helianthus annus L.).
(2)
The most efficient absorption of the heavy metal Zn was on the T0K0 treatment (100% soil and 0 mg/kg EDTA) with the total of Zn concentration of 101.74 mg/kg in plants. The T0K0 treatment is capable of reducing Zn weight by up to 96% of total Zn available on the soil.
(3)
The addition of EDTA chelating agents affects the concentration of the heavy metal Zn on the sunflower plant tissue. The greater the addition of EDTA, the greater the concentration of heavy metals in the sunflower plant tissue. The addition of EDTA to 100% of the soil causes the decrease in the absorption efficiency of the heavy metal Zn by sunflower plants. The addition of EDTA to 40% sludge did not affect the absorption of the heavy metal Zn by sunflower plants.



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*Source: 8259520-2018-05-22.xml*