# Formation of Aldehydic Phosphatidylcholines during the Anaerobic Decomposition of a Phosphatidylcholine Bearing the 9-Hydroperoxide of Linoleic Acid

Authors: Arnold N. Onyango
Journal: BioMed Research International
 (2016)

---

## Abstract

Lipid oxidation-derived carbonyl compounds are associated with the development of various physiological disorders. Formation of most of these products has recently been suggested to require further reactions of oxygen with lipid hydroperoxides. However, in rat and human tissues, the formation of 4-hydroxy-2-nonenal is greatly elevated during hypoxic/ischemic conditions. Furthermore, a previous study found an unexpected result that the decomposition of a phosphatidylcholine (PC) bearing the 13-hydroperoxide of linoleic acid under a nitrogen atmosphere afforded 9-oxononanoyl-PC rather than 13-oxo-9,11-tridecadienoyl-PC as the main aldehydic PC. In the present study, products of the anaerobic decomposition of a PC bearing the 9-hydroperoxide of linoleic acid were analysed by electrospray ionization mass spectrometry. 9-Oxononanoyl-PC (ONA-PC) and several well-known bioactive aldehydes including 12-oxo-9-hydroperoxy-(or oxo or hydroxy)-10-dodecenoyl-PCs were detected. Hydrolysis of the oxidized PC products, methylation of the acids obtained thereby, and subsequent gas chromatography-mass spectroscopy with electron impact ionization further confirmed structures of some of the key aldehydic PCs. Novel, hydroxyl radical-dependent mechanisms of formation of ONA-PC and peroxyl-radical dependent mechanisms of formation of the rest of the aldehydes are proposed. The latter mechanisms will mainly be relevant to tissue injury under hypoxic/anoxic conditions, while the former are relevant under both normoxia and hypoxia/anoxia.


---

## Body

## 1. Introduction

Lipid oxidation results in the formation of a plethora of products with diverse biological activities relevant to human health [1–3]. Autoxidation of linoleic acid, the most abundant polyunsaturated fatty acid in mammalian tissues, affords two monohydroperoxides, namely, 13-hydroperoxy-9,11-octadecadienoic acid (13-LA-OOH,1 in Scheme 1) and 9-hydroperoxy-10,12-octadecadienoic acid (9-LA-OOH,2) [3–5]. Decomposition of these hydroperoxides under aerobic conditions readily affords hexanal3 and 9-oxononanoic acid4 [4, 5]. In addition, 13-LA-OOH1 affords 4-hydroperoxy-2-nonenal5 as a major product which decomposes to form 4-hydroxy-2-nonenal6 and 4-oxo-2-nonenal7 [4, 6]. Decomposition of hydroperoxide1 also affords, as minor products, 12-oxo-9-hydroperoxy-10-dodecenoic acid8, a precursor of 12-oxo-9-hydroxy (or oxo)-10-dodecenoic acids9 and10, respectively [4]. Conversely, 9-LA-OOH2 affords oxo-acids8–10 as major products and aldehydes5–7 as minor products [4].Scheme 1
Some major products of decomposition of the 13- and the 9-hydroperoxides of linoleic acid (13-LA-OOH1 and 9-LA-OOH2, resp.).Ironically, inadequate oxygen supply to tissues elevates oxidative stress and results in greater generation of lipid oxidation products, which contribute to tissue damage. For example, exposing rat heart to ischemia led to a 50-fold increase in 4-hydroxy-2-nonenal, and there was no further increase in this aldehyde upon reperfusion [7]. Elevated levels of 4-hydroxy-2-nonenal6 during hypoxia/ischemia lead to severe myocardial toxicity [8], and renal ischemia/reperfusion injury is a major cause of acute renal failure [9]. 9-Oxononanoic acid4 and its phospholipid esters may not be as chemically reactive as aldehydes6–10, but it contributes to inflammation by quickly inducing the activity of phospholipase A2 (PLA2), the key enzyme for initiating arachidonic acid cascade and eicosanoid production [10, 11]. Moreover, cardiolipin hydrolysis by mitochondrial secretory PLA2 disrupts the respiratory chain and increases the production of reactive oxygen species [12]. Thus, an understanding of the mechanisms of formation of aldehydic products such as3–10 under both normoxia and hypoxia-anoxia is necessary.Although great progress has recently been made in elucidating the mechanisms of formation of these compounds under aerobic conditions [13–15], not much has been done regarding their formation under anaerobic conditions, with most of the suggested mechanisms requiring further reactions of linoleic acid hydroperoxides with molecular oxygen. However, it was previously found that decomposition of a phosphatidylcholine bearing the 13-hydroperoxide of linoleic acid (PC-13-LA-OOH) under anaerobic conditions led to the formation of 9-oxononanoyl-PC as the major aldehydic PC even though the latter cannot be directly formed by β-scission of the alkoxyl radical derived from PC-13-LA-OOH [16]. In the present study, a phosphatidylcholine bearing 9-LA-OOH2 was decomposed under anaerobic conditions (nitrogen atmosphere) and the decomposition products analysed by electrospray ionization mass spectroscopy (ESI-MS), which readily gives molecular ion peaks of oxidatively modified phospholipids without the need for prior separation [14]. Peaks for various well-known aldehydic phospholipid products were detected, and their structures were confirmed by GC-MS with electron impact ionization. Based on the codetection of the aldehydic PCs with their potential precursors or coproducts by ESI-MS, novel mechanisms of their formation are proposed.


## 2. Materials and Methods

### 2.1. General

1-Stearoyl-2-lyso-sn-3-glycerophosphocholine (lyso PC) was purchased from Avanti Polar Lipids (Alabaster, AL), soybean lipoxygenase and linoleic acid were purchased from Nacalai Tesque (Kyoto, Japan), FeSO4·7H2O was purchased from Kanto Chemical Co. (Tokyo, Japan), and TLC was done on precoated silica gel 60 F254 plates (Merck, Art 5554). Column chromatography was done on silica gel 60. NMR spectra were recorded on a Varian VXR-500 instrument. ES-MS measurements were taken on an API III triple quadruple mass spectrometer (PE-Sciex, Thorn Hill, ON, Canada).


### 2.2. Preparation of 1-Stearoyl-2-(9′-hydroperoxy-10′,12′-octadecadienoyl)-sn-glycero-3-phosphocholine (PC-9-LA-OOH11)

1-Stearoyl-2-(9′-hydroperoxy-10′,12′-octadecadienoyl)-sn-glycero-3-phosphocholine (PC-9-LA-OOH) was prepared as previously described [14]. Briefly, the 9-hydroperoxide of linoleic acid (9-LA-OOH) was prepared by oxidation of linoleic acid with a crude homogenate of tomato fruit as described by Schneider et al. [4]. 9-LA-OOH was protected as a peracetal and esterified to lyso-PC, after which the peracetal group was hydrolysed [14, 17]. After column chromatography, PC-9-LA-OOH was obtained as a resinous solid, and its structure was confirmed by 1H NMR and ESI-MS. 1H NMR (500 MHz, CDCl3) δ 0.87 (6H, m, CH3 ×2), 1.28 (44H, m, CH2 ×22), 1.58 (4H, m, OCOCH2CH2 in each chain), 2.15 (2H, m, 8′-H), 2.28 (4H, t, OCOCH2 in each chain), 3.30 (9H, s, NMe3), 3.75 (2H, m, CH2N), 3.98 (2H, m, OCH2-CH-CH2O), 4.14 (1H, m, one proton of OCH2-CH-CH2O), 4.34 (4H, one proton of OCH2-CH-CH2O, OPOCH2, 13′-H), 5.22 (1H, m, CO-CH-CO), 5.44 (1H, m, 9-H), 5.57 (1H, dd, H-12), 5.97 (1H, t, 10-H), 6.46 (1H, dd, 11-H). ESI-MS: Found,m/z 818 (M + H+ requires 818).


### 2.3. Decomposition of PC-9-LA-OOH11 under Anaerobic and Aerobic Conditions

PC-9-LA-OOH (6 mg, 7.3μmol) was dissolved in 12 mL chloroform. After addition of FeSO4·7H2O, (1 mg, 3.6 μmol), the air in the reaction flask was evacuated under vaccuum and replaced with nitrogen. The evacuation and nitrogen replacement was repeated three times. The reaction mixture was stirred at 37°C in the dark under the nitrogen atmosphere (a balloon filled with nitrogen was connected to the mouth of the reaction flask). Aliquots were taken at different time points and analysed by ESI-MS. A trace of the antioxidant BHT was added to the aliquots to minimize further oxidations before analysis. For decomposition of PC-9-LA-OOH under aerobic conditions, oxygen replacement with nitrogen was not done.


### 2.4. Derivatization of PC-9-LAOOH Decomposition Products with Methoxylamine Hydrochloride

200μL aliquots taken from the decomposition mixture of PC-9-LA-OOH were evaporated in vacuo and the residue was reacted with methoxylamine hydrochloride (2.5 mg in 100 μL of pyridine) at 37°C for 2 hours in the dark [18].


### 2.5. Electrospray Ionization Mass Spectroscopy (ESI-MS)

ESI-MS analysis was performed on an API III triple quadruple mass spectrometer (PE-Sciex, Thorn Hill, ON, Canada) equipped with an electrospray interface. Aliquots taken from the decomposition experiment were diluted with THF-MeOH-H2O to less than 50 ppm and introduced directly into the spectrometer with a microsyringe (250 μL) through an infusion pump and a fused silica capillary tubing (0.25 mm diameter) at a rate of 5 μL/min. Spectra were acquired in the positive ion mode with the orifice at 70 V. Both normal scans for protonated molecular ion peaks and scans for the parents ofm/z 184 (corresponding to the phosphocholine ion characteristic to all PCs) were done.


### 2.6. Hydrolysis and GC-MS of PC-9-LA-OOH Decomposition Products

The mixtures of PC-9-LA-OOH decomposition products obtained as described above were hydrolysed and the acids obtained thereby converted to methyl esters prior to GC-MS as described previously for identification of the decomposition products from a PC bearing the 13-hydroperoxide ofα-linolenic acid [18]. The hydrolysis was done in 1 M HCl at 80°C for 30 minutes, followed by adjustment of the pH to 5 and subsequent extraction with ethyl acetate. The solvent was subsequently evaporated and the residue treated with ethereal diazomethane for 2 min. After further solvent evaporation, the residue was dissolved in ethyl acetate and submitted for GC MS analysis on a Hewlett Packard 5890 series II GC fitted with a DB-1 (30 m × 0.25 mm) column and coupled to an automass 20-mass detector. The injector and detector temperatures were set at 250°C and the column temperature was programmed from 80°C (5 minutes) to 230°C (10 minutes) at 10°C/min. Compound identification was based on comparison of their retention times and mass spectra with those obtained from the mass spectral reference library of National Institute of Standards and Technology as well as those found in literature.


## 2.1. General

1-Stearoyl-2-lyso-sn-3-glycerophosphocholine (lyso PC) was purchased from Avanti Polar Lipids (Alabaster, AL), soybean lipoxygenase and linoleic acid were purchased from Nacalai Tesque (Kyoto, Japan), FeSO4·7H2O was purchased from Kanto Chemical Co. (Tokyo, Japan), and TLC was done on precoated silica gel 60 F254 plates (Merck, Art 5554). Column chromatography was done on silica gel 60. NMR spectra were recorded on a Varian VXR-500 instrument. ES-MS measurements were taken on an API III triple quadruple mass spectrometer (PE-Sciex, Thorn Hill, ON, Canada).


## 2.2. Preparation of 1-Stearoyl-2-(9′-hydroperoxy-10′,12′-octadecadienoyl)-sn-glycero-3-phosphocholine (PC-9-LA-OOH11)

1-Stearoyl-2-(9′-hydroperoxy-10′,12′-octadecadienoyl)-sn-glycero-3-phosphocholine (PC-9-LA-OOH) was prepared as previously described [14]. Briefly, the 9-hydroperoxide of linoleic acid (9-LA-OOH) was prepared by oxidation of linoleic acid with a crude homogenate of tomato fruit as described by Schneider et al. [4]. 9-LA-OOH was protected as a peracetal and esterified to lyso-PC, after which the peracetal group was hydrolysed [14, 17]. After column chromatography, PC-9-LA-OOH was obtained as a resinous solid, and its structure was confirmed by 1H NMR and ESI-MS. 1H NMR (500 MHz, CDCl3) δ 0.87 (6H, m, CH3 ×2), 1.28 (44H, m, CH2 ×22), 1.58 (4H, m, OCOCH2CH2 in each chain), 2.15 (2H, m, 8′-H), 2.28 (4H, t, OCOCH2 in each chain), 3.30 (9H, s, NMe3), 3.75 (2H, m, CH2N), 3.98 (2H, m, OCH2-CH-CH2O), 4.14 (1H, m, one proton of OCH2-CH-CH2O), 4.34 (4H, one proton of OCH2-CH-CH2O, OPOCH2, 13′-H), 5.22 (1H, m, CO-CH-CO), 5.44 (1H, m, 9-H), 5.57 (1H, dd, H-12), 5.97 (1H, t, 10-H), 6.46 (1H, dd, 11-H). ESI-MS: Found,m/z 818 (M + H+ requires 818).


## 2.3. Decomposition of PC-9-LA-OOH11 under Anaerobic and Aerobic Conditions

PC-9-LA-OOH (6 mg, 7.3μmol) was dissolved in 12 mL chloroform. After addition of FeSO4·7H2O, (1 mg, 3.6 μmol), the air in the reaction flask was evacuated under vaccuum and replaced with nitrogen. The evacuation and nitrogen replacement was repeated three times. The reaction mixture was stirred at 37°C in the dark under the nitrogen atmosphere (a balloon filled with nitrogen was connected to the mouth of the reaction flask). Aliquots were taken at different time points and analysed by ESI-MS. A trace of the antioxidant BHT was added to the aliquots to minimize further oxidations before analysis. For decomposition of PC-9-LA-OOH under aerobic conditions, oxygen replacement with nitrogen was not done.


## 2.4. Derivatization of PC-9-LAOOH Decomposition Products with Methoxylamine Hydrochloride

200μL aliquots taken from the decomposition mixture of PC-9-LA-OOH were evaporated in vacuo and the residue was reacted with methoxylamine hydrochloride (2.5 mg in 100 μL of pyridine) at 37°C for 2 hours in the dark [18].


## 2.5. Electrospray Ionization Mass Spectroscopy (ESI-MS)

ESI-MS analysis was performed on an API III triple quadruple mass spectrometer (PE-Sciex, Thorn Hill, ON, Canada) equipped with an electrospray interface. Aliquots taken from the decomposition experiment were diluted with THF-MeOH-H2O to less than 50 ppm and introduced directly into the spectrometer with a microsyringe (250 μL) through an infusion pump and a fused silica capillary tubing (0.25 mm diameter) at a rate of 5 μL/min. Spectra were acquired in the positive ion mode with the orifice at 70 V. Both normal scans for protonated molecular ion peaks and scans for the parents ofm/z 184 (corresponding to the phosphocholine ion characteristic to all PCs) were done.


## 2.6. Hydrolysis and GC-MS of PC-9-LA-OOH Decomposition Products

The mixtures of PC-9-LA-OOH decomposition products obtained as described above were hydrolysed and the acids obtained thereby converted to methyl esters prior to GC-MS as described previously for identification of the decomposition products from a PC bearing the 13-hydroperoxide ofα-linolenic acid [18]. The hydrolysis was done in 1 M HCl at 80°C for 30 minutes, followed by adjustment of the pH to 5 and subsequent extraction with ethyl acetate. The solvent was subsequently evaporated and the residue treated with ethereal diazomethane for 2 min. After further solvent evaporation, the residue was dissolved in ethyl acetate and submitted for GC MS analysis on a Hewlett Packard 5890 series II GC fitted with a DB-1 (30 m × 0.25 mm) column and coupled to an automass 20-mass detector. The injector and detector temperatures were set at 250°C and the column temperature was programmed from 80°C (5 minutes) to 230°C (10 minutes) at 10°C/min. Compound identification was based on comparison of their retention times and mass spectra with those obtained from the mass spectral reference library of National Institute of Standards and Technology as well as those found in literature.


## 3. Results and Discussion

The profile of products of the decomposition of PC-9-LAOOH obtained by ESI-MS depended on the time when an aliquot was taken for analysis. Figures1(a) and 1(b) are sample spectra taken after 4 hours of reaction under anaerobic and aerobic conditions, respectively. The peaks in these spectra are parents ofm/z 184 (corresponding to the phosphocholine ion) in the parent ion scan mode, confirming that they are all oxidatively modified phosphatidylcholines. Since the parent hydroperoxide PC-9-LAOOH is of known structure, and its common decomposition products are known [14], most of the peaks in Figure 1 can be easily assigned.Figure 1
ESI-MS spectra obtained after decomposition of PC-9-LAOOH for 4 hours in the presence of Fe2+ under (a) anaerobic and (b) aerobic conditions.
(a)
(b)Before decomposition, PC-9-LA-OOH (11 in Scheme 2) gave a single ESI-MS peak atm/z 818 (not shown). However, the peak atm/z 818 in Figure 1(a) should at least partly also belong to 9,10-epoxy-11-hydroxy-12-octadecenoyl-PC and 9,10-epoxy-13-hydroxy-11-octadecenoyl-PC because the decomposition of linoleic acid hydroperoxides in the presence of ferrous ions generates such hydroxy-epoxides [19, 20]. Morever, there is a peak atm/z 836, which is 18 amu higher than 818, and is consistent with dihydroxy PCs formed by hydrolysis of the mentioned epoxy-hydroxy-PCs. Epoxy-hydroxy acids are also generated from linoleic acid hydroperoxides in the presence of hematin or lipoxygenases under anaerobic conditions [21–23].Scheme 2
Suggested conversion of PC-9-LA-OOH11 to secondary products by in-cage reaction of alkoxyl radical12 or epoxy allyl radical13 with O
∙
H formed by a reverse Fenton reaction betwen Fe3+ and −OH.The hematin-mediated conversion of linoleic acid hydroperoxides to epoxy-hydroxy acids was proposed to involve the ferric ion (Fe3+) of hematin reacting with the hydroperoxide (ROOH) to form F
e
4
+
O
-
H and an alkoxyl radical (R
O
∙), followed by the alkoxyl radical cyclizing to an epoxy allylic radical, which subsequently couples to hydroxyl radical (O
∙
H) derived from F
e
4
+
O
-
H, while the latter is still within the “solvent cage”, in the so-called oxygen-rebound mechanism [21]. A similar mechanism is involved in the lipoxygenase-mediated formation of the hydroxy epoxides [22, 23]. P. Spiteller and G. Spiteller [19] found that the 9,10-epoxy-11-hydroxy-12-octadecenoic acid generated during the anaerobic Fe2+-mediated decomposition of 9-LA-OOH also retained both oxygen atoms from the hydroperoxide and that molecular oxygen was not required for its formation. They proposed that the epoxy-hydroxy acid was formed by conversion of the hydroperoxide to the corresponding peroxyl radical, which then transfers one oxygen atom to the 11-position. However, the mechanism for such oxygen transfer is unclear.Lipinski [24] recently reported that free ferric ions oxidize hydroxide ions to generate hydroxyl radicals, in a special kind of Fenton reaction:(1)
F
e
3
+
+
O
H
-
⟶
F
e
2
+
+
O
H
∙Therefore, as illustrated in Scheme 2, the Fe2+-mediated decomposition of PC hydroperoxide11 should involve initial conversion of the latter to alkoxyl radical12, with coformation of Fe3+ and −OH, followed by cyclization of12 to afford epoxy allylic radical13, whose combination with O
H
∙ derived from F
e
3
+
O
-
H pair produces 9,10-epoxy-11-hydroxy-12-octadecenoyl-PC14 (m/z 818) or 9,10-epoxy-13-hydroxy-11-octadecenoyl-PC (not shown).The peak atm/z 800 (Figure 1(a)) corresponds to 9-oxo-10,12-octadecadienoyl-PC15 (Scheme 2), and it is more prominent than a peak atm/z 802, corresponding to 9-hydroxy-10,12-octadecadienoyl-PC16. This result is similar to other studies of the decomposition of linoleoyl hydroperoxides where oxodiene formation is always higher than hydroxydiene formation [14, 25] unless there is excess of a reducing agent [19]. It is reasonable to consider that the O
H
∙ generated from the F
e
3
+
O
-
H pair does not selectively add to epoxyallyl radical13 but it abstracts a hydrogen atom from alkoxyl radical12 to form oxodiene-PC15, and this should at least partly explain the greater formation of the latter than hydroxydiene PC16.The prominent peak atm/z 678 belongs to 9-oxononanoyl-PC (9-ONA-PC,17). Derivatization with methoxylamine hydrochloride shifted this peak by 29 amu tom/z 707 (not shown), thus confirming the carbonyl group [14, 18]. Other aldehydes mentioned hereafter were similarly derivatized by methoxylamine hydrochloride. The major peak atm/z 694 belongs to 9-carboxy-nonanoyl-PC (azelaoyl-PC) formed by oxidation of ONA-PC17, according to the known conversion of aldehydes to their corresponding acids under anaerobic conditions [18].It is unlikely that alkoxyl radical12 underwent β-scission to form 9-ONA-PC17 and vinylic radical18 (Scheme 2). Although such a reaction is commonly claimed in the literature, argument has been raised against it because vinylic radicals are unstable and their formation is highly energetically unfavourable [25–27]. On the other hand, β-scission of a hydroperoxydiene-derived alkoxyl radical to form an allylic radical and a 2,4-dienal is facile, while β-scission to form an alkyl radical and a 2,4-dienal occurs to a small extent [27]. Thus, β-scission of alkoxyl radical12 occurs to a small extent to afford 2,4-decadienal and octanoyl radical19. Oxygen rebound from F
e
3
+
O
-
H to radical19 affords 8-hydroxy-octanoyl-PC (not shown), for which a peak is present atm/z 666. This is the same mechanism for lipoxygenase-mediated formation of short chain alcohols from hydroperoxides under anaerobic conditions [28]. If radical19 abstracts a hydrogen atom instead, octanoyl PC will be formed, and a small peak consistent with this compound is present atm/z 650. If, on the other hand,19 combines with a peroxyl radical, a peroxide will be formed, whose decomposition will produce the alkoxyl radical derivative of19, whose decomposition may afford 8-oxooctanoyl-PC (m/z 664). However, there are other possible ways of formation of the latter (vide infra).Gardner and Plattner [26] suggested that the facile formation of 9-oxononanoic acid from 9-LA-OOH may occur by the heterolytic Hock cleavage mechanism. However, Hock cleavage requires strong acidic conditions and is therefore not relevant for the facile formation of 9-ONA-PC17 under the conditions of the present study.Grechkin et al. [29] reported that a hydroperoxide lyase from water melon could catalyze the isomerization of 9-LA-OOH2 to a hemiacetal via the corresponding epoxy allylic radical and a vinyl ether radical, and that the hemiacetal cleaves to afford 9-oxononanoic acid and 3-nonenal. The nonenzymatic rearrangement of the epoxy allylic radical to a vinyl ether radical is also known [30]. Thus, as suggested in Scheme 2, a likely pathway for the formation of 9-ONA-PC17 in the present study involves conversion of epoxy allylic radical13 to vinyl ether radical20, followed by oxygen rebound from the F
e
3
+
O
-
H pair to form hemiacetal21, whose rearrangement affords ONA-PC17 and 3-nonenal. Furthermore, similar to the known reduction of the alkoxyl radical to alkoxide ion by Fe2+ [19], Fe2+ might reduce vinyl ether radical20 to carbanion22, which would cleave to form ONA-PC17 and vinylic anion23, because, unlike vinylic radicals which are unstable, vinylic anions are relatively stable [31]. Vinylic anion23 will then be converted to 1,3-nonadiene24, a known product of the oxidation of sunflower oil [32] whose formation has otherwise been speculated to involve unstable vinylic radical18 [25].Since hydroxyl radicals also undergo addition to double bonds, the hydroxyl radical derived fromF
e
3
+
O
-
H pair may alternatively add to alkoxyl radical12 to form biradical25 which would easily cleave to form ONA-PC17 and dienol26 which would then rearrange to form 3-nonenal.Unoxidized 1-stearoyl-2-linoleoyl-PC would havem/z 783. Therefore all peaks occurring betweenm/z 783 andm/z 694 (for 9-carboxy-nonanoyl-PC) belong to products having oxidatively shortened acyl chains of at least 10 carbons. As suggested in Scheme 3, formation of such aldehydes under anaerobic conditions may depend on the addition of peroxyl radicals to PC-LA-OOH11, since such peroxyl radical additions are known to form dimers and even trimers [25], whose decomposition affords aldehydic products [13, 33].Scheme 3
Proposed anaerobic pathways for conversion of PC-9-LA-OOH11 to aldehyde-PCs other than 9-oxononanoyl-PC. Conversion of32 to33 may begin with Fe2+-mediated or spontaneous cleavage of32 to the corresponding alkoxyl radical, which cyclizes to form an epoxy allylic radical, which adds a peroxide radical, whose decomposition affords33.The peroxyl radicals may be formed by the alkoxyl radical or ferric ion-mediated oxidation of hydroperoxide molecules [19, 25]:(2)
R
O
O
H
+
R
O
∙
⟶
R
O
O
∙
+
R
O
H
(3)
F
e
3
+
+
R
O
O
H
⟶
R
O
O
∙
+
F
e
2
+
+
H
+Thus, peroxyl radical addition to PC-9-LA-OOH11 affords dimeric radical27, which may be converted to trimer28, whose decomposition would afford hexanal3 and 12-oxo-9-hydroperoxy-10-dodecenoyl-PC29 (m/z 750). This is based on the mechanism proposed to occur under aerobic conditions, where radical27 reacts with O2 and is eventually converted to a dihydroperoxy-peroxide whose cleavage affords3 and29 [13]. The peak atm/z 766 corresponds to 12-carboxy-9-hydroperoxy-10-dodecenoyl-PC, the corresponding acid of29.Hydroperoxy aldehyde-PC29 may decompose to form 9,12-dioxo-10-dodecenoyl-PC or 9-hydroxy-12-oxo-10-dodecenoyl-PC, with peaks atm/z 732 and 734, respectively, and the corresponding acids of the latter two have peaks atm/z 748 and 750, respectively.A peroxide linked dimeric radical such as27 decomposes to form an alkoyl radical and an epoxide [13, 25]. Hence27 may be converted to epoxy-hydroperoxy-PC30, for which there is a matching peak atm/z 834. The peak atm/z 704 is consistent with 11-oxo-9-undecenoyl-PC, whose formation requires displacement of the hydroperoxy group from carbon 9 of the linoleoyl chain. This is speculated to partly involve rearrangement of dimeric radical27 to31, followed by expulsion of a hydroperoxyl radical (H
O
O
∙) from the latter to form peroxide-linked dimer32. Similar expulsion of the H
O
O
∙ may have been involved in the recently reported conversion of PC-13-LA-OOH (a PC bearing 13-LA-OOH1) to an octadecatrienoyl-PC on human skin [34]. Cleavage of the O-O bond in peroxide32 affords an alkoxy radical whose further cyclization and reaction with a peroxyl radical may lead to formation of epoxyalkoxyl radical33, whose β-scission affords 11-oxo-9-undecenoyl-PC34 (m/z 704) and an oxiranyl radical. β-scission of epoxy-alkoxyl radicals such as33 may be facile because the oxiranyl radicals are oxygen-stabilized [14].Gardner and Plattner [26] found a related result that methyl 11-oxo-9-undecenoate was a product of the thermal decomposition of methyl 12,13-epoxy-9-hydroperoxy-10-octadecenoate. The mechanism they proposed for the conversion of the latter to the former may also apply to the conversion of epoxy-hydroperoxide30 to 11-oxo-undecenoyl-PC34. This involves conversion of30 to the corresponding peroxyl radical (not shown), whose β-scission affords the corresponding epoxy allylic radical, which adds a peroxyl radical at C-11 to form a dimeric epoxy-peroxide, whose O-O cleavage affords epoxyalkoxyl radical33 as a precursor of34.β-Scission of epoxy-alkoxyl radical35, formed from PC-9-LA-OOH11 via epoxy allylic radical13, will produce 2-octenal and oxiranyl radical36, which may abstract a hydrogen atom to form 9,10-epoxy-decanoyl-PC37 (m/z 692). This is not a well-known product, but the detection of epoxides is not always easy because of their reactivity, including their susceptibility to hydrolysis [18, 25]. The peak atm/z 710 is consistent with 9,10-dihydroxy-decanoyl-PC38 formed by hydrolysis of epoxy-PC37.Oxiranyl radicals such as36 undergo oxidation to oxiranoxy radicals such as39 [35]. Hydrogen abstraction by39 may then lead to formation of 9-hydroxy-10-oxo-decanoyl-PC40 (m/z 708). 9-Hydroxy-10-oxo-decanoic acid is a known product of the decomposition of 9-LA-OOH [19]. Moreover, 2-hydroxy-heptanal, the corresponding hydroxyaldehyde from 13-LA-OOH, is a major product formed during the autoxidation of n-6 fatty acids such as linoleic acid [36] and is generated at comparable level to 4-hydroxy-2-nonenal6 [37]. Notably, the peak for 9-hydroxy-10-oxodecanoyl-PC39 (m/z 708) has a relative intensity comparable to that of 9-hydroxy-12-oxo-10-dodecenoyl-PC (m/z 734) in Figure 1(a).The peak for 9-hydroxy-10-carboxy-decanoyl-PC, expected from the oxidation of hydroxy-aldehyde PC40, is negligible (m/z 724) in Figure 1(a). This is understandable because oxidation of aldehydes begins by their conversion to the corresponding acyl radicals [14, 18], which may undergo decarbonylation [38]. The radical formed by decarbonylation of40 is oxygen-stabilized, and this may favour decarbonylation over conversion to an acid, especially when it is not readily trapped by oxygen to form a peracid (vide infra) as would happen under aerobic conditions.Further oxidation of 11-oxo-undecenoyl-PC34 via alkoxyl radical41 will not only lead to formation of 8-oxooctanoyl-PC42 (m/z 664) as the main product, but β-scission of alkoxyl radical41 may also lead to formation of heptanoyl radical as a precursor of 7-oxo-heptanoyl-PC (m/z 650).The peak atm/z 860 (Figure 1) belongs to a product whose identity is not known at the moment but is most likely a decomposition product of a dimer, possibly a C-C linked dimer.GC-MS of the mixture of products obtained by hydrolysis of PC-9-LA-OOH decomposition products, followed by methylation of the resultant acids, helped to confirm the structures of some of the compounds whose molecular ion peaks are shown in Figure1. Table 1 shows some of the detected methyl esters and their key fragment ions. Their identification was based on matching spectra in the commercial spectral library, except for methyl 9,12-dioxo-10-dodecenoate, which was compared with other literatures [18]. The detection of methyl-9-oxononanoate confirms the identity of 9-oxononanoyl-PC 17 (m/z 678 in Figure 1), nonanedioc acid dimethyl ester confirms 9-carboxy-oxononanoyl-PC (m/z 694), methyl-9,12-dioxo-10-dodecenoate confirms 9,12-dioxo-10-dodecenoyl-PC (m/z 732), and methyl stearate is derived fromsn-1 position of all the PC-9-LAOOH-derived products. Methyl 8-(2-furyl)octanoate indirectly confirms the formation of 9-hydroxy-12-oxo-10-dodecenoyl-PC (m/z 734), since the latter is known to undergo cyclization into a hemiacetal that dehydrates to form 8-(2-furyl)octanoyl-PC [39, 40]. Since no peak for 8-(2-furyl)octanoyl-PC was observed atm/z 716 in the ESI-MS spectrum (Figure 1), it is likely that the formation of this compound occurred during the acidic hydrolysis of PC-9-LA-OOH decomposition products.Table 1
Methyl esters detected by GC-MS after hydrolysis of PC-9-LA-OOH decomposition products under anaerobic conditions.
Compound
R.T (min)
Amplitude (%)
Key fragment ions
Methyl 9-oxononanoic acid
13.23
25
43 (48), 55 (65), 69 (30), 74 (100), 87 (65), 111 (40), 143 (20), 155 (12), 158 (6)
Nonanedioic acid dimethyl ester
15.03
27
43 (48), 55 (100), 83 (90), 97 (37), 111 (52), 124 (23), 143 (23), 152 (62), 185 (38)
Methyl 8-(2-furyl)-octanoate
19.44
12.5
41 (12), 53 (20), 67 (6), 81 (100), 95 (50), 123 (10), 193 (2), 224 (5)
Methyl 9,12-dioxo-10-dodecenoate
20.12
8
41 (20), 55 (85), 98 (100), 130 (63), 181 (5), 209 (25), 240 (3)
Methyl stearate
22.21
100
43 (50), 55 (30), 69 (16), 74 (100), 87 (62), 143 (13), 199 (10), 255 (12), 298 (11)Unfortunately, structures of the rest of the compounds mentioned in Schemes2 and 3 could not be confirmed by GC-MS, most likely due to side reactions they underwent during hydrolysis and/or methylation or their requirement for alternative derivatization procedures. For example, although methyl-9-oxononanoate was detected, the structurally similar methyl-8-oxo-octanoate was not. It is possible that chances for the detection of methyl-9-oxononanoate were increased by it being generated in much larger amounts than the rest of the aldehydic products.It was previously reported that, during the decomposition of a PC bearing the 13-hydroperoxide ofα-linolenic acid (PC-13-LNA-OOH) under anaerobic conditions similar to that used in the present study, the formation of products such as 12-oxo-9-hydroperoxy-(or oxo- or hydroxy)-10-dodecenoyl-PCs was negligible even though such products were significant under aerobic conditions. The difference between the decomposition of PC-13-LNA-OOH and the decomposition of PC-9-LA-OOH is that β-scission of the alkoxyl radical derived from the former is facile, so that its major decomposition product is 13-oxo-9,11-tridecadienoyl-PC, which is the main precursor of the 12-oxo-9-hydroperoxy (or hydroxy- or oxo)-10-dodecenoyl-PCs under aerobic conditions but is instead largely converted to 13-carboxy-9,11-tridecadienoyl-PC under anaerobic conditions [18]. Thus, although it is highly likely that a small amount of oxygen remained in the reaction flask during decomposition of PC-9-LA-OOH in the present study and could have participated to some extent in product formation, the oxygen-independent reactions proposed in Scheme 3 must have played an important role.For comparison, the ESI-MS spectrum obtained by decomposition of PC-9-LA-OOH for 4 hours under aerobic conditions is shown in Figure1(b). Many of the peaks are common to Figures 1(a) and 1(b). However, the peak atm/z 800 for 9-oxo-10,11-octadecadienoyl-PC15 is greatly diminished in Figure 1(b), although it was major during earlier stages of the aerobic decomposition. Its time-dependent disappearance corresponds with enhancement in the peak atm/z 694 for azelaoyl-PC. A potential pathway for the conversion of oxo-PC15 (m/z 800) to azelaoyl-PC is suggested to begin with hydrogen abstraction to form radical43 whose reaction with oxygen affords a peroxyl radical which finally gets converted to alkoxyl radical44, whose β-scission affords 2,4-nonadienal and relatively stable carbonyl radical45 (Scheme 4). The latter is then converted to peracid46 which undergoes a Bayer-Villiger reaction with another aldehyde molecule (RCHO) to form azelaoyl PC47 [14]. Alternatively, peracid46 decomposes to the corresponding acyl radical (not shown), which abstracts a hydrogen to form azelaoyl-PC47 [14].Scheme 4
Suggested pathway for the conversion of 9-oxo-10,11-octadecadienoyl-PC15 to azelaoyl-PC47 under aerobic conditions.Another difference between the aerobic decomposition and anaerobic decomposition of PC-9-LA-OOH is the enhanced formation of a peak atm/z 816 under aerobic conditions (Figure 1(b)). Under aerobic conditions, epoxy allylic radical13 is expected to be readily converted to epoxy-hydroperoxy-PC48 and subsequently to 9,10-epoxy-13-oxo-PC49 (m/z 816) (Scheme 5), as opposed to anaerobic conditions, where oxygen rebound to radical13 and the formation of epoxy-hydroxy-PC14 (m/z 818) are favoured (Scheme 2).Scheme 5
The conversion of PC-9-LA-OOH to epoxy-oxo derivative49 under aerobic conditions.From the mechanisms of formation of 9-oxononanoyl-PC17 in Scheme 2, it can be deduced that hydroxyl radical scavenging antioxidants will reduce its formation, with an associated decreases in PLA2-dependent proinflammatory factors. Likewise, peroxyl radical scavengers should lower the formation of various aldehydes according to Scheme 3. The polyphenol resveratrol and the peptide carnosine are examples of compounds that decrease ischemia induced tissue injury [41, 42], which may partly be attributed to them being good hydroxyl radical scavengers [24, 43]. Carnosine is also a scavenger of reactive aldehydes such as 4-hydroxy-2-nonenal [44].


## 4. Conclusions

The decomposition of a phosphatidylcholine bearing the 9-hydroperoxide of linoleic acid under anaerobic conditions was found to easily form aldehydic products including 9-oxononanoyl-phosphatidylcholine as a predominant product, 9-hydroperoxy-(or oxo or hydroxy)-12-oxo-10-dodecenoyl-PCs, and compounds putatively identified as 9-hydroxy-10-oxo-decanoyl-PC, 11-oxo-9-undecenoyl-PC, and 8-oxooctanoyl-PC. Novel mechanisms of their formation are proposed, involving a key role of hydroxyl and peroxyl radicals. This information will be important when considering antioxidant strategies, especially targeting hypoxia-induced oxidative stress. However, more studies will be required to confirm the identities of compounds that were not confirmed by GC-MS in the present study.



---

*Source: 8218439-2016-06-05.xml*