# Thorough Characterization of Brazilian New Generation of Eucalypt Clones and Grass for Pulp Production

Authors: Fernando José Borges Gomes; Jorge Luiz Colodette; Auphélia Burnet; Larisse Aparecida Ribas Batalha; Fernando Almeida Santos; Iara Fontes Demuner
Journal: International Journal of Forestry Research
 (2015)

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## Abstract

Eucalypt wood is becoming the most important raw material for the pulp industries in South America. However, due to the high wood cost in comparison to other raw material sources, nonwoody materials are also being investigated aiming at pulp production. In this way, this paper aimed at the evaluation of eighteen eucalypt clones obtained from the Brazilian Genolyptus project, regarding their potential characteristics for pulp production. Aiming at the same goal, two species of elephant grass were also evaluated as alternative raw material sources. Through the analyses of the anatomic and chemical characteristics, five eucalypt clones and one elephant grass species were indicated for pulp production and biorefinery application. The results of this study indicate the high technological quality ofEucalyptus clones evaluated and indicate that they can be used for biorefinery applications since they have the suitable characteristics. In general, the eucalypt clones are less moist and denser and contain fewer minerals and extraneous materials than the elephant grass species, which make them more attractive for utilization in deconstruction studies aiming at production of bioproducts.


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## Body

## 1. Introduction

Biomass sources such as hardwood and softwood are the main raw materials for biorefinery application, since they are largely used in the pulp mills and deliver the suitable characteristics to the final products with competitive costs. In this context, eucalypt is becoming the most important raw material. The major interest in eucalypt wood comes from its relative low production cost in certain regions, for example, in South America, due mainly to high forest productivity and high pulping yield.It is very well known that wood quality is a factor of extreme importance when the goal is the pulp production with high industrial yield, low cost, and high quality. Wood characteristics like fiber anatomy and chemical composition are expected to affect its processability, that is, ease of delignification and the quality of the pulp products [1, 2]. Chemical structures may vary among different wood species and even among eucalypt clones. Many studies have been done in order to increase wood productivity and improve its quality aiming at the pulp production through the selection of clones with better performance and crossings between them [3, 4]. Good results have been observed; for example, the average productivity of the Brazilian eucalypt forests increased from 24 m3/ha/yr in 1980 to 41 m3/ha/yr in 2010, representing a 71% increase in productivity of planted forests in Brazil [5].In spite of wood to be a consolidated raw material for the pulp industry and biorefinery applications, alternative sources of biomass have been investigated to wood replacement, for example, elephant grass. High productivity plants such as elephant grass (30–45 bone dry t/ha/yr) can potentially supply biomass of low cost to meet the current demand [6–12]. However, the quality of such raw material aiming at pulp production is not yet fully known. Elephant grass has special characteristics for the pulp production, which are the high fiber production, similar to sugar cane [13, 14], and its chemical composition. Some works have showed a content of 40%, 30%, and 17.7% for cellulose, hemicelluloses, and lignin, respectively [15].Among Brazilian pulp industries, research institutes, and universities, a research project was developed, which aimed at eucalypts forest improvements for pulp production, called Brazilian Genolyptus. In this study, eighteen eucalypt clones obtained from the Brazilian Genolyptus project were investigated regarding their potential characteristics for pulp production. Aiming at the same goal, two species of elephant grass were also evaluated as alternative source for the pulp industry. Through the analysis of the anatomic and chemical characteristics, five eucalypt clones and one elephant grass species were indicated for pulp production.


## 2. Materials and Methods

### 2.1. Materials

For this study, 18 eucalypt clone samples in commercial cutting age were investigated provided byGENOLYTPUS project, located in Minas Gerais State, Brazil, and also two species of elephant grass (150 days old) were studied. The complete list of samples used in this study is presented in Table 1.Table 1
Codification of the eucalypt clones and elephant grass species.
Sample code
Biomass type
1
U1xU2
E. urophylla(Flores IP) x  E. urophylla(Timor)
2
U2xC1
E. urophylla(Timor) x E. camaldulensis(VM1)
3
G1xUGL
E. grandis(Coffs Harbour) x  [E. urophylla(R) x E. globulus(R)]
4
U1xUGL
E. urophylla(Flores IP) x  [E. urophylla(R) x E. globulus(R)]
5
U1xC2
E. urophylla(Flores IP) x E. camaldulensis (VM2)
6
C1xC2
E. camaldulensis(VM1) x E. camaldulensis(VM1)
7
DGxUGL1
[
E. dunnii(R) x E. grandis(R)]  x [E. urophylla(R) x E. globulus(R)]
8
DGxU2
[
E. dunnii(R) x E. grandis(R)]  x E. urophylla(Timor)
9
C1xUGL
E. camaldulensis(VM1) x  [E. urophylla(R) x E. globulus(R)]
10
G1xGL2
E. grandis(Coffs Harbour) x E. globulus(R)
11
DGxC1
[
E. dunnii(R) x E. grandis(R)]  x E. camaldulensis(VM1)
12
U2xGL1
E. urophylla(Timor) x E. globulus(R)
13
DGxGL2
[
E. dunnii(R) x E. grandis(R)]  x E. globulus(R)
14
U1xD2
E. urophylla(Flores IP) x E. dunnii(R)
15
U1xG2
E. urophylla(Flores IP) x E. grandis
16
IP
E. urophylla(IP) x E. grandis(IP) commercial clone
17
VC
E. urophylla x E. grandiscommercial clone
18
CC
E. urophylla x E. grandiscommercial clone
19
EG1
Pennisetum purpureum
20
EG2
Pennisetum americanumFive trees of each eucalypt clone, with DBH (diameter at breast height: 1.4 m) and heights corresponding to the average population, were harvested, split into five 50 cm long bolts at 0, 25, 50, 75, and 100% of the tree heights, and a 100 kg sample of each elephant grass species was harvested at a Federal University of Viçosa experimental station. The samples were evaluated for their moisture content at the moment of harvesting according to Tappi T264 cm-07 standard procedure [16]. The eucalypt clones samples were chipped in a laboratory chipper, a Chogokukikai model, equipped with 3 knives and 2 screens (40 and 13 mm). Both eucalypt clones and elephant grass chips were well mixed (260 m3 rotary mixer) and the eucalypt clones samples were screened according to SCAN-CN 40:94 procedure [17]. For eucalypt clones samples, the chips retained in the 3 mm and 7 mm screens were collected and mixed again, air dried to about 15% of moisture, and stored in large plastic bags. The elephant grass samples were manually chipped, producing wet chips about 5 mm long. The chip thickness was quite variable since it depended on the grass thickness, which varies from 1 cm diameter to 4 cm diameter. The grass chips were air dried to a moisture content of about 15% and stored in large plastic bags.


### 2.2. Methods

#### 2.2.1. Biomass Density

The chips were used to measure chips bulk and basic density according to SCAN CN-49:92 [18] and SCAN CM-46:92 [19] standard procedures, respectively.


#### 2.2.2. Biomass Productivity

The biomass productivity was calculated using the medium annual increase (MAI) and basic density, by the following equation:(1)Biomassproductivityton/ha/yr=MAIm3/ha/yr×basicdensity(ton/m3).


#### 2.2.3. Samples Preparation for Morphological Analysis

About 200 grams of each biomass sample was sliced into toothpick type material and macerated using a solution of nitric and acetic acid in order to prepare individual fibers for morphological analyses. To make the maceration, a solution of five parts of acetic acid and one part of nitric acid were mixed, added to the biomass material until they were completely immersed and then maintained for 6 hours at 100°C under a hood. The reaction was stopped by washing the material, which was dispersed in distilled water. Then, the material was gently mixed in a magnetic stirrer (slowly and steadily) for 60 min, so that all the fiber bundles were separated. Morphological characterization of fibers, vessels, and fines was carried out on a pulp suspension passing through a specific cell illuminated by a laser beam and connected to a high-resolution camera (CCD). This analysis allowed reliable statistical measurement of thousands of fibers, vessels, and fines to determine the main morphological and dimensional characteristics of the pulp components.


#### 2.2.4. Samples Preparation for Chemical Analysis

For the chemical analyses, about 1 kg of each biomass was sampled and ground in a Wiley type mill to produce sawdust of variable size. This sawdust was screened according to Tappi Standard T257-cm12 [20]. The sawdust that passed the 40-mesh screen and was retained in the 60-mesh screen was selected for the chemical analyses. The sawdust was air dried and conditioned in a temperature and humidity controlled room (23±1°C, 50±2% RH) until an equilibrium moisture was achieved (~10%). This sawdust (raw sawdust) was used for the chemical analyses. The analyses of ash, silica, chloride, iron, copper, manganese, potassium, calcium, and magnesium were carried out directly on the raw sawdust, according to the Standard Methods for the Examination of Water and Wastewater [21], except for chloride, which was determined according to Tappi T256 cm-07 standard procedure [22]. The biomass extractives contents in acetone, ethanol/toluene (1 : 2), and ethanol/toluene (1 : 2) → ethanol → hot water solvent series were also determined in the raw sawdust using the TAPPI T280 pm-99 [23], T204 cm-97 [24], and TAPPI T264 cm-07 [25] standard procedures, respectively. In order to determine biomass main cell wall components, a 200 g sample of biomass extractives free was prepared using TAPPI T264 cm-07 [16] standard procedure. This extracted sample (extractive free sawdust) was conditioned in a temperature and humidity controlled room (23±1°C, 50±2% RH) until an equilibrium moisture was achieved (~10%). The contents of uronic acids, acetyl groups, and sugars (glucans, mannans, galactans, xylans, and arabinans) in the extractive free biomass were determined according to Scott [25], Solar et al. [26], and Wallis et al. [27]. The acid insoluble lignin, acid soluble lignin, and lignin syringyl/guaiacyl (S/G) ratio were determined according to TAPPI T 222 om-97 standard procedure [28], Goldschmid [29], and Lin and Dence [30], respectively.


## 2.1. Materials

For this study, 18 eucalypt clone samples in commercial cutting age were investigated provided byGENOLYTPUS project, located in Minas Gerais State, Brazil, and also two species of elephant grass (150 days old) were studied. The complete list of samples used in this study is presented in Table 1.Table 1
Codification of the eucalypt clones and elephant grass species.
Sample code
Biomass type
1
U1xU2
E. urophylla(Flores IP) x  E. urophylla(Timor)
2
U2xC1
E. urophylla(Timor) x E. camaldulensis(VM1)
3
G1xUGL
E. grandis(Coffs Harbour) x  [E. urophylla(R) x E. globulus(R)]
4
U1xUGL
E. urophylla(Flores IP) x  [E. urophylla(R) x E. globulus(R)]
5
U1xC2
E. urophylla(Flores IP) x E. camaldulensis (VM2)
6
C1xC2
E. camaldulensis(VM1) x E. camaldulensis(VM1)
7
DGxUGL1
[
E. dunnii(R) x E. grandis(R)]  x [E. urophylla(R) x E. globulus(R)]
8
DGxU2
[
E. dunnii(R) x E. grandis(R)]  x E. urophylla(Timor)
9
C1xUGL
E. camaldulensis(VM1) x  [E. urophylla(R) x E. globulus(R)]
10
G1xGL2
E. grandis(Coffs Harbour) x E. globulus(R)
11
DGxC1
[
E. dunnii(R) x E. grandis(R)]  x E. camaldulensis(VM1)
12
U2xGL1
E. urophylla(Timor) x E. globulus(R)
13
DGxGL2
[
E. dunnii(R) x E. grandis(R)]  x E. globulus(R)
14
U1xD2
E. urophylla(Flores IP) x E. dunnii(R)
15
U1xG2
E. urophylla(Flores IP) x E. grandis
16
IP
E. urophylla(IP) x E. grandis(IP) commercial clone
17
VC
E. urophylla x E. grandiscommercial clone
18
CC
E. urophylla x E. grandiscommercial clone
19
EG1
Pennisetum purpureum
20
EG2
Pennisetum americanumFive trees of each eucalypt clone, with DBH (diameter at breast height: 1.4 m) and heights corresponding to the average population, were harvested, split into five 50 cm long bolts at 0, 25, 50, 75, and 100% of the tree heights, and a 100 kg sample of each elephant grass species was harvested at a Federal University of Viçosa experimental station. The samples were evaluated for their moisture content at the moment of harvesting according to Tappi T264 cm-07 standard procedure [16]. The eucalypt clones samples were chipped in a laboratory chipper, a Chogokukikai model, equipped with 3 knives and 2 screens (40 and 13 mm). Both eucalypt clones and elephant grass chips were well mixed (260 m3 rotary mixer) and the eucalypt clones samples were screened according to SCAN-CN 40:94 procedure [17]. For eucalypt clones samples, the chips retained in the 3 mm and 7 mm screens were collected and mixed again, air dried to about 15% of moisture, and stored in large plastic bags. The elephant grass samples were manually chipped, producing wet chips about 5 mm long. The chip thickness was quite variable since it depended on the grass thickness, which varies from 1 cm diameter to 4 cm diameter. The grass chips were air dried to a moisture content of about 15% and stored in large plastic bags.


## 2.2. Methods

### 2.2.1. Biomass Density

The chips were used to measure chips bulk and basic density according to SCAN CN-49:92 [18] and SCAN CM-46:92 [19] standard procedures, respectively.


### 2.2.2. Biomass Productivity

The biomass productivity was calculated using the medium annual increase (MAI) and basic density, by the following equation:(1)Biomassproductivityton/ha/yr=MAIm3/ha/yr×basicdensity(ton/m3).


### 2.2.3. Samples Preparation for Morphological Analysis

About 200 grams of each biomass sample was sliced into toothpick type material and macerated using a solution of nitric and acetic acid in order to prepare individual fibers for morphological analyses. To make the maceration, a solution of five parts of acetic acid and one part of nitric acid were mixed, added to the biomass material until they were completely immersed and then maintained for 6 hours at 100°C under a hood. The reaction was stopped by washing the material, which was dispersed in distilled water. Then, the material was gently mixed in a magnetic stirrer (slowly and steadily) for 60 min, so that all the fiber bundles were separated. Morphological characterization of fibers, vessels, and fines was carried out on a pulp suspension passing through a specific cell illuminated by a laser beam and connected to a high-resolution camera (CCD). This analysis allowed reliable statistical measurement of thousands of fibers, vessels, and fines to determine the main morphological and dimensional characteristics of the pulp components.


### 2.2.4. Samples Preparation for Chemical Analysis

For the chemical analyses, about 1 kg of each biomass was sampled and ground in a Wiley type mill to produce sawdust of variable size. This sawdust was screened according to Tappi Standard T257-cm12 [20]. The sawdust that passed the 40-mesh screen and was retained in the 60-mesh screen was selected for the chemical analyses. The sawdust was air dried and conditioned in a temperature and humidity controlled room (23±1°C, 50±2% RH) until an equilibrium moisture was achieved (~10%). This sawdust (raw sawdust) was used for the chemical analyses. The analyses of ash, silica, chloride, iron, copper, manganese, potassium, calcium, and magnesium were carried out directly on the raw sawdust, according to the Standard Methods for the Examination of Water and Wastewater [21], except for chloride, which was determined according to Tappi T256 cm-07 standard procedure [22]. The biomass extractives contents in acetone, ethanol/toluene (1 : 2), and ethanol/toluene (1 : 2) → ethanol → hot water solvent series were also determined in the raw sawdust using the TAPPI T280 pm-99 [23], T204 cm-97 [24], and TAPPI T264 cm-07 [25] standard procedures, respectively. In order to determine biomass main cell wall components, a 200 g sample of biomass extractives free was prepared using TAPPI T264 cm-07 [16] standard procedure. This extracted sample (extractive free sawdust) was conditioned in a temperature and humidity controlled room (23±1°C, 50±2% RH) until an equilibrium moisture was achieved (~10%). The contents of uronic acids, acetyl groups, and sugars (glucans, mannans, galactans, xylans, and arabinans) in the extractive free biomass were determined according to Scott [25], Solar et al. [26], and Wallis et al. [27]. The acid insoluble lignin, acid soluble lignin, and lignin syringyl/guaiacyl (S/G) ratio were determined according to TAPPI T 222 om-97 standard procedure [28], Goldschmid [29], and Lin and Dence [30], respectively.


## 2.2.1. Biomass Density

The chips were used to measure chips bulk and basic density according to SCAN CN-49:92 [18] and SCAN CM-46:92 [19] standard procedures, respectively.


## 2.2.2. Biomass Productivity

The biomass productivity was calculated using the medium annual increase (MAI) and basic density, by the following equation:(1)Biomassproductivityton/ha/yr=MAIm3/ha/yr×basicdensity(ton/m3).


## 2.2.3. Samples Preparation for Morphological Analysis

About 200 grams of each biomass sample was sliced into toothpick type material and macerated using a solution of nitric and acetic acid in order to prepare individual fibers for morphological analyses. To make the maceration, a solution of five parts of acetic acid and one part of nitric acid were mixed, added to the biomass material until they were completely immersed and then maintained for 6 hours at 100°C under a hood. The reaction was stopped by washing the material, which was dispersed in distilled water. Then, the material was gently mixed in a magnetic stirrer (slowly and steadily) for 60 min, so that all the fiber bundles were separated. Morphological characterization of fibers, vessels, and fines was carried out on a pulp suspension passing through a specific cell illuminated by a laser beam and connected to a high-resolution camera (CCD). This analysis allowed reliable statistical measurement of thousands of fibers, vessels, and fines to determine the main morphological and dimensional characteristics of the pulp components.


## 2.2.4. Samples Preparation for Chemical Analysis

For the chemical analyses, about 1 kg of each biomass was sampled and ground in a Wiley type mill to produce sawdust of variable size. This sawdust was screened according to Tappi Standard T257-cm12 [20]. The sawdust that passed the 40-mesh screen and was retained in the 60-mesh screen was selected for the chemical analyses. The sawdust was air dried and conditioned in a temperature and humidity controlled room (23±1°C, 50±2% RH) until an equilibrium moisture was achieved (~10%). This sawdust (raw sawdust) was used for the chemical analyses. The analyses of ash, silica, chloride, iron, copper, manganese, potassium, calcium, and magnesium were carried out directly on the raw sawdust, according to the Standard Methods for the Examination of Water and Wastewater [21], except for chloride, which was determined according to Tappi T256 cm-07 standard procedure [22]. The biomass extractives contents in acetone, ethanol/toluene (1 : 2), and ethanol/toluene (1 : 2) → ethanol → hot water solvent series were also determined in the raw sawdust using the TAPPI T280 pm-99 [23], T204 cm-97 [24], and TAPPI T264 cm-07 [25] standard procedures, respectively. In order to determine biomass main cell wall components, a 200 g sample of biomass extractives free was prepared using TAPPI T264 cm-07 [16] standard procedure. This extracted sample (extractive free sawdust) was conditioned in a temperature and humidity controlled room (23±1°C, 50±2% RH) until an equilibrium moisture was achieved (~10%). The contents of uronic acids, acetyl groups, and sugars (glucans, mannans, galactans, xylans, and arabinans) in the extractive free biomass were determined according to Scott [25], Solar et al. [26], and Wallis et al. [27]. The acid insoluble lignin, acid soluble lignin, and lignin syringyl/guaiacyl (S/G) ratio were determined according to TAPPI T 222 om-97 standard procedure [28], Goldschmid [29], and Lin and Dence [30], respectively.


## 3. Discussion and Results

### 3.1. Forestry Characteristics

Two very important factors regarding biomass use for pulp production are moisture content and density since they affect harvesting, transportation, and utilization costs. The eucalypt clones analyzed in this study show average moisture and density of 55% and 502 kg/m3. These values are considered satisfactory for pulp production [31–34]. The MAI varied in the range of 16–101.6 m3/ha/yr. The lowest MAI extreme occurred for the C1xC2 woody raw material. This may be explained due to the fact that eucalypt hybrid is poorly adapted to Minas Gerais State climate conditions and did not develop satisfactorily. Among the woody raw materials, the highest growth (101.6 m3/ha/yr) was obtained with sample DGxU2, which is a triple hybrid of (Eucalyptus dunnii xEucalyptus grandis) xEucalyptus urophylla (Table 2). This productivity is much above the average MAI obtained in commercial plantations in the Brazilian Territory (~40–60 m3/ha/yr) [5].Table 2
Forestry and physical characteristics of the eucalypt clones and elephant grass species evaluated.
Sample code
Moisture, %
Average annual increment, m3/ha/yr*
Biomass basic density, kg/m3
Biomass productivity, bone dry ton/ha/yr
Chip bulk density, kg/m3
U1xU2
54
86.0
504
43.3
209
U2xC1
55
54.1
547
29.6
220
G1xUGL
53
46.2
500
23.1
202
U1xUGL
54
46.9
496
23.3
193
U1xC2
53
52.9
517
27.4
203
C1xC2
54
16.0
533
8.5
207
DGxUGL1
55
57.7
449
25.9
193
DGxU2
56
101.6
496
50.4
203
C1xUGL
53
19.9
519
10.3
220
G1xGL2
54
39.3
530
20.8
211
DGxC1
53
72.6
500
36.3
213
U2xGL1
52
40.1
506
20.3
208
DGxGL2
55
28.5
489
13.9
197
U1xD2
54
42.6
441
18.8
178
U1xG2
56
63.4
518
32.8
228
IP
55
80.9
480
38.8
183
VC
54
80.3
473
38.0
179
CC
55
40.2
472
19.0
183
EG1
73
148.1
216
32.0
54
EG2
75
140.0
200
28.0
75
*Data provided by the feedstock suppliers.


### 3.2. Morphological Characteristics

Another important aspect of the raw material for pulp production is its morphological characteristics. The strength and morphology of fibers have a strong influence on the physical properties of paper as well as the wood pulpability [1, 2, 35]. In Table 3, the morphological characterizations of all the evaluated samples are presented.Table 3
Fiber and vessels characterization of the eucalypt clones and elephant grass species evaluated.
Samplecode
Fibre content, millions/g of pulp
Mean fibre arithmetic length,μm
Mean length-weighted fibre length,μm
Mean area-weighted length,μm
Mean fibre width,μm
Mean fibre coarseness, mg/m
Mean fibre curl index, %
Macrofibrillation index, %
Broken fibre content, %
Fine content, % in area
Mean fine area,μm2
Mean fine length,μm
Vessel content, nb/g of pulp
Mean area-weighted length, mm
Mean vessel width,μm
U1xU2
29.8
617.5
746.5
742.5
16.0
0.05
4.5
0.48
14.6
7.8
1707
65.5
8132
0.41
186.5
U2xC1
26.2
692.5
843.5
847.0
15.2
0.05
4.3
0.42
14.1
7.2
1666
64.5
3730
0.31
181.2
G1xUGL
27.9
635.0
779.5
775.0
16.5
0.05
5.0
0.45
15.0
8.0
1661
66.0
10795
0.44
185.0
U1xUGL
26.5
637.0
788.0
786.5
17.0
0.06
4.0
0.43
16.0
9.0
1590
64.0
3699
0.45
189.0
U1xC2
32.1
579.0
732.0
729.5
16.1
0.05
5.0
0.55
16.0
11.0
1537
63.0
3523
0.33
185.0
C1xC2
33.5
601.0
753.0
749.0
15.2
0.04
5.5
0.51
14.6
7.5
1594
64.0
3232
0.41
182.0
DGxUGL1
36.6
584.0
729.0
731.5
18.8
0.04
5.0
0.56
18.4
8.8
1512
61.0
1580
0.34
178.1
DGxU2
31.6
622.5
763.0
768.0
17.6
0.05
4.2
0.49
16.4
8.0
1574
61.5
1774
0.32
182.8
C1xUGL
35.8
631.5
791.0
789.5
15.0
0.04
6.1
0.50
14.3
6.8
1499
61.5
2756
0.30
181.4
G1xGL2
30.5
621.0
755.0
752.0
15.9
0.05
4.9
0.47
14.7
8.0
1534
61.5
3502
0.36
188.7
DGxC1
26.1
655.0
790.5
789.5
16.1
0.05
4.4
0.39
15.3
8.0
1663
63.0
7857
0.35
188.1
U2xGL1
37.5
589.0
717.5
719.5
15.7
0.04
5.4
0.58
15.0
7.4
1479
60.5
2891
0.43
183.5
DGxGL2
29.4
567.0
703.0
705.5
18.2
0.06
5.0
0.53
19.4
12.2
1462
58.5
2942
0.37
182.7
U1xD2
31.2
591.5
736.0
740.5
18.8
0.05
4.4
0.54
18.4
9.0
1469
59.0
8484
0.44
183.3
U1xG2
27.8
629.5
766.5
767.0
17.3
0.05
4.4
0.48
15.3
8.9
1751
64.5
10434
0.35
189.3
IP
28.1
562.0
733.0
733.5
16.4
0.06
4.5
0.54
17.7
12.4
1647
64.5
6771
0.43
194.3
VC
30.0
579.0
729.5
733.0
17.3
0.05
4.6
0.52
17.9
9.0
1578
63.5
3101
0.47
186.6
CC
33.8
551.5
692.5
695.5
18.3
0.05
5.1
0.57
18.1
11.6
1633
64.5
7546
0.45
190.2
EG1
14.9
695.5
1131
1114
21.5
0.09
6.8
0.42
26.1
11.9
1987
62
11628
0.45
191.1
EG2
21.7
686.0
1113
1059
19.1
0.06
6.0
0.45
20.6
11.1
2748
73
12023
0.61
205.6It was observed that the fiber content depends on the coarseness; the lower coarseness, the higher fiber content. The fiber coarseness is lower for samples U2xGL1, C1xC2, DGxUGL1, and C1xUGL and higher for samples DGxGL2, IP, and U1xUGL. This fiber coarseness measurement gives indication on the number of fibers required to reach a given basic weight and therefore affects the runnability of the paper machines, for example. The longest mean area-weighted fiber length is obtained for hybrid eucalypt clone U2xC1, followed by samples DGxC1, UG, DGxU2, G1xUGL, U1xUGL, and C1xUGL. The eucalypt clones DGxGL2 and CC have the shortest fiber. The length of the fiber has an influence on the mechanical properties of the final paper [36]. Longer fiber improves mechanical properties of the final paper. Curl index is higher for samples UxGL1, CC, C1xC2, and C1xUGL. This characteristic indicates that the fibers of these samples are less flexible than those of other eucalypt clone fibers. Macrofibrillation index and broken fiber content give information on the state of the fiber before deconstruction pretreatment or pulping. The CC and DGxUGL1 clones presented more broken fiber and the corresponding fiber is more fibrillated than the other clones. The clones DGxGL2, IP, and CC contain a higher amount of broken fiber which can explain the higher amount of fines on these eucalypt clones. For the pulping process, the vessel elements are desirable, since the penetration of cooking liquors is easier. However, for the production of special kinds of paper, such as printing papers, they are considered undesirable, because the vessel on the surface of the paper sheet tends to be pulled thereby causing printing failures; this is known as “vessels picking” [37]. Eucalypt clones DGxC1, U1xU2, U1xD2, U1xG2, G1xUGL, and CC have higher vessels content than the other samples. These vessels' content can be correlated to the growth rate of the tree, as, for example, the higher wood production, the higher vessels content. This means that, for the choice of the best clones to proceed with the study, the length of the fiber and the vessels content are the most important characteristics to help in the choice of the clones that should be used for the cooking process. Hybrid eucalypt clones C1xUGL, U1xUGL, and DGxUGL1 seem interesting because some characteristics, such as vessels content, fiber length, broken fiber, and fines content, are adequate for quality of papers.Regarding elephant grass samples, a lower fiber content per gram in EG1 and EG2 samples was observed. The width, length, coarseness, fine content, and macrofibrillation index may be considered close to the eucalypt samples. However, the EG1 and EG2 showed a high vessel content. Vessels are recognized to generate problems during printing as explained previously [37]. But in general it is possible to say that the fibers have potential for paper production, since the characteristics desired for paper quality are observed.


### 3.3. Chemical Characteristics

Table4 shows the extractive quantity of the eucalypt clones and elephant grass samples extracted with the ethanol/toluene → ethanol → hot water solvent series, with ethanol/toluene 1 : 2 only and with acetone. In order to measure the biomass cell wall components, it is relevant to remove all extractives present in the material. The Tappi T204 CM 97 standard procedure [24] (ethanol/toluene 1 : 2 → ethanol → hot water) was efficient for removing all polar and apolar extractive fractions. Although this procedure is intended to free the wood from extractives, it serves also to quantify the total amount of extractives present in the biomass, since the main cell wall components (cellulose, hemicelluloses, and lignin) are not soluble in any of the solvents comprising the series. Extraction with ethanol/toluene only extracts substances as waxes, fats, resins, phytosterols, and nonvolatile hydrocarbons. Extraction in acetone [23] serves to quantify those extractives that are more relevant to the pulping operation and pitch formation in the pulp. The acetone extractable content of wood is a measure of such substances as fatty acids, resin acids, sterols, waxes, and nonvolatile hydrocarbons. Because acetone is both more polar and water-miscible than dichloromethane or benzene-ethanol, the quantity of acetone extractable material, especially in wood, may be higher than that found with the other solvents. This procedure will not give the same results as ethanol-toluene or dichloromethane extractions. In their work, Barbosa et al. [38] showed that acetone is the best solvent for the evaluation of the wood lipophilic extract content.Table 4
Extractive content of the eucalypt clones and elephant grass species evaluated.
Sample code
Acetone extractives, %
Ethanol/toluene (1 : 2) extractives, %
Total extractives, %
U1xU2
1.7
1.9
3.6
U2xC1
1.1
1.4
3.4
G1xUGL
2.5
2.5
4.9
U1xUGL
1.9
1.3
3.6
U1xC2
1.2
1.2
2.8
C1xC2
1.2
1.3
3.0
DGxUGL1
1.2
2.7
2.8
DGxU2
1.2
2.2
2.7
C1xUGL
0.9
1.6
2.1
G1xGL2
1.2
1.9
2.8
DGxC1
0.9
1.2
1.9
U2xGL1
1.0
1.4
2.5
DGxGL2
1.1
1.7
1.9
U1xD2
2.3
2.3
3.0
U1xG2
2.1
1.6
2.8
IP
0.8
1.5
2.3
VC
1.6
2.6
3.7
CC
1.1
2.6
3.5
EG1
3.9
6.8
14.8
EG2
9.9
1.8
17.6Biomass extractives are quite troublesome since they cause many difficulties in operating the industrial facilities, causing unexpected lost time in the operation for cleaning of equipment and instruments due to their stickiness and tackiness. In addition the deposition of these substances in the pulp may occur, which are called pitch [38], decreasing the pulp value or even its rejection by market. In their study Cruz et al. [39] showed that in pitch composition there are waxes, fats, and long chain alcohols, being the main compounds associated with pitch formation [40].For the pulp production, among the eucalypt clones acceptable total extractive contents (1.9 to 4.9%) were observed for all clones [34, 41]. On the other hand, the EG samples showed a high content of acetone extractives and ethanol/toluene extractives. In addition, pitch formation and pulp dirtiness are much more likely on raw materials containing high content of these extractives. As a consequence, the EG1 and EG2 also presented a high total extractive content (14.8% and 17.6, resp.) since such materials are likely to result in low yield during cooking process.The raw material minerals are detrimental for their industrial utilization, since they cause corrosion and deposits on equipment, reduce biomass heating value, and decrease mill throughput. In Table5 the results of biomass mineral content are presented. In general the amounts of inorganics present in the eucalypt clones were very low and quite acceptable for most applications [42, 43]. For the eucalypt clones, the total inorganics measured by complete biomass combustion (ash content) varied in the range of 950–2,510 mg/kg biomass. For the EG1 and EG2, they reached 60, 100, and 37,900 mg/kg, respectively. The very high mineral content in the grass is explained by its fast metabolism at the young age when it needs plenty of minerals to produce the biomass. Another interesting fact about the contents of minerals in biomass is that they tend to decrease with aging due to decreased biomass deposition rate as a function of time [44]. The same trend observed for total inorganics (ash content) is also verified for the individual components such as silica, chloride, calcium, potassium, and magnesium, with the elephant grass samples always presenting higher values than the eucalypt clone samples. Calcium, magnesium, and silica are very undesirable in most industrial processes because of their ability to cause deposits in equipment during evaporation of liquid streams and combustion of solid streams. On the other hand, potassium and chloride are particularly dangerous for their ability to decrease the ash melting point during combustion, thus causing sticky ash problems in recovery boiler systems [45]. In addition, chlorides are highly corrosive and troublesome for most equipment regardless of metallurgy. In regard to transition metals (Fe, Cu, and Mn), there were also significant differences among the eucalypt clones and elephant grass materials, again always presenting the same trend, higher content in elephant grass samples.Table 5
Ash and metal content of the eucalypt clones evaluated.
Sample code
Inorganics, mg/kg biomass
Ash
Cu
Fe
Ca
Mn
Mg
K
Cl−
U1xU2
950.0
0.8
15.5
307.0
9.5
81.0
194.0
260.0
U2xC1
1850.0
0.7
12.3
384.0
16.0
146.0
265.0
328.0
G1xUGL
1950.0
0.5
13.1
452.0
14.0
112.0
188.0
629.0
U1xUGL
1200.0
1.2
14.6
394.0
9.8
148.0
185.0
454.0
U1xC2
1250.0
0.7
10.3
328.0
9.7
144.0
340.0
594.0
C1xC2
2510.0
1.1
8.2
518.0
19.8
156.0
404.0
413.0
DGxUGL1
1950.0
0.8
18.2
532.0
16.3
118.0
358.0
500.0
DGxU2
2280.0
1.2
13.5
531.0
13.2
123.0
218.0
427.0
C1xUGL
2160.0
0.6
10.6
627.0
23.6
166.0
435.0
523.0
G1xGL2
1790.0
0.6
19.5
525.0
18.9
129.0
450.0
446.0
DGxC1
1400.0
0.9
10.6
263.0
11.2
128.0
252.0
701.0
U2xGL1
1800.0
0.7
13.5
475.0
14.3
136.0
152.0
519.0
DGxGL2
1750.0
1.0
11.3
462.0
15.0
123.0
202.0
600.0
U1xD2
1400.0
0.8
14.8
264.0
10.9
115.0
222.0
689.0
U1xG2
1850.0
1.1
20.4
296.0
11.2
92.0
384.0
477.0
IP
1550.0
1.1
9.3
378.0
18.2
104.0
369.0
434.0
VC
1800.0
1.4
9.9
491.0
2.2
123.0
469.0
488.0
CC
1700.0
1.2
8.5
356.0
13.9
183.0
370.0
399.0
EG1
60100.0
8.8
11.2
423
11.1
490.0
21194
15167.0
EG2
37900.0
3.8
67.5
352
56.5
1201.0
16055
2335.0In Table6 the biomass chemical composition is presented. There were significant variations among the results of contents of sugars, acetyl group, uronic acids, lignin, and syringyl/guaiacyl ratio of lignin. Among the eucalypt clones, the total lignin contents varied in the range of 27.1 to 31.3%. The maximum value was obtained for the double crossing U1xC2 hybrid and the minimum for the G1xGL2 one. However, these values are considered acceptable for eucalypt clones, but for pulp production a lower lignin content and high S/G ratio are desired due to the increase of the pulpability of the wood [46]. About the carbohydrate content, the eucalypt clones presented values considered satisfactory for eucalypts aiming at pulp production [34].Table 6
Chemical composition of the eucalypt clones and elephant grass species evaluated.
Sample code
Sugar composition, %
Acid soluble lignin %
Total lignin %
Lignin S/G ratio
Acetyl group %
Uronic acid group   %
Glucan
Xylans
Galactans
Mannans
Arabinans
U1xU2
46.1
11.8
0.8
0.8
0.2
4.3
30.3
2.8
2.1
3.7
U2xC1
45.5
10.7
1.2
1.0
0.2
4.6
30.8
2.7
1.9
4.0
G1xUGL
43.9
13
0.8
0.9
0.2
4.7
28.9
2.9
2.7
3.8
U1xUGL
44.9
11.7
1.1
0.8
0.2
5
29.7
3.1
2.4
4.0
U1xC2
45.6
10
1.4
1.1
0.2
4.5
31.3
3.0
1.8
3.8
C1xC2
45.6
9.7
1.6
0.9
0.3
5.1
31.1
3.0
1.6
4.1
DGxUGL1
45.5
13
0.9
0.8
0.3
5.3
29.2
3.2
2.6
4.0
DGxU2
45.3
12.6
0.9
1.0
0.3
4.4
29.8
2.6
2.5
4.0
C1xUGL
46.0
11.9
1.1
0.9
0.3
5.0
30.7
3.2
2.2
4.0
G1xGL2
46.4
14.1
1.0
0.8
0.3
5.0
27.1
3.5
3.0
3.9
DGxC1
47.2
10.8
1.2
0.9
0.3
4.7
31.0
2.8
1.8
4.0
U2xGL1
45.5
13.4
1.2
0.8
0.3
5.6
29.3
3.8
2.6
4.0
DGxGL2
45.8
12.9
1.1
1.0
0.3
4.4
28.2
2.9
2.5
3.8
U1xD2
48.1
11.4
1.0
0.9
0.3
4.4
28.6
2.6
2.0
3.9
U1xG2
46.8
11.8
1.0
0.8
0.3
4.4
30.2
2.6
2.0
4.0
IP
49.4
12
1.2
0.9
0.3
4.2
27.2
2.7
1.9
4.0
VC
46.9
11.4
0.8
1.1
0.2
4.6
28.4
2.9
2.1
3.8
CC
47.4
11.2
1.0
1.1
0.2
4.1
28.4
2.4
1.9
3.9
EG1
38.2
9.6
0.8
0.6
0.2
2.2
18.0
0.8
1.9
1.2
EG2
35.6
16.1
0.5
ND
1.6
1.6
16.5
0.7
1.8
1.4Regarding elephant grass, the lignin contents for EG1 and EG2 biomass were 18 and 16.5%, respectively. The low lignin content of the elephant grass samples is advantageous for potentially improving pulping easiness and yield. However, its low lignin S/G ratio and low sugar content work in the opposite direction [46]. The low sugar content of the elephant grass samples reflected their very high extractive and mineral contents.


### 3.4. Some Relations among Cell Wall Components

In order to determine lignin content, it is necessary to treat biomass with strong acids so that the carbohydrate fraction is hydrolyzed, leaving lignin as a residue. This acid hydrolysis procedure leads to severe lignin condensation and precipitation so that it is easily collected from the solution through simple filtration. Some of the lignin, particularly in hardwoods, seemingly condenses insufficiently in the acid media, becomes soluble, and sieves through the filtration system. This lignin is the so-called acid soluble lignin. Although acid soluble lignin is negligible for softwood biomass, it is quite important for hardwoods. The amount of acid soluble lignin in the woody biomass investigated varied in the range of 4.1–5.6% of the wood dry weight (Table5). Although not commonly investigated, the amount of acid soluble lignin seems to be related to the lignin S/G ratio. Lignin containing larger amounts of syringyl monomers will condense less during the strong acid hydrolysis treatment, since the C5 position in the aromatic ring is blocked in the syringyl units. These blocked C5 positions prevent C5 condensation. Therefore, lignin containing high S/G ratio, that is, high number of syringyl units, will condense less during the acid hydrolysis procedure and produce more soluble lignin in the filtrates. Figure 1 shows that there is very good correlation between lignin S/G ratio and acid soluble lignin content for the various woody and nonwoody biomass, in agreement with the proposed theory.Figure 1
Correlation between biomass acid soluble lignin and lignin S/G ratio.


### 3.5. Raw Material Selection

Based on all the analyses, the best five eucalypt clones and one elephant grass selected for the pulp production were(1)
a double crossing hybrid ofEucalyptus urophylla xEucalyptus urophylla (U1xU2), that was selected on the basis of its very high annual growth (83 m3/ha/yr), high wood density, excellent morphological traits, very high forest yield (43 ton/ha/yr), and low xylan and uronic acid contents;(2)
a triple crossing hybrid ofEucalyptus grandis x (Eucalyptus urophylla xEucalyptus globulus) (G1xUGL), which presented a high xylan content and possessedEucalyptus globulus in its genotype, which is of interest for high S/G ratio, although it is quite challenging for its high content of extractives (4.9%);(3)
a triple crossing of (Eucalyptus dunnii xEucalyptus grandis) xEucalyptus urophylla (DGxU2), that was selected due to its highest annual growth (101 m3/ha/yr) among all eucalypts evaluated, good density, outstanding morphological traits, and the highest forest yield (~50 ton/ha/yr);(4)
a commercial elite clone (IP) that was obtained from a large Brazilian forest company, which is a double crossing ofEucalyptus urophylla xEucalyptus grandis, for its excellent forest productivity (38.5 ton/ha/yr), good density, the highest cellulose content, and lowest lignin content among all and also for being a very good reference since it is commercially planted by a large pulp company in Brazil;(5)
regarding the elephant grass, the EG1 (Pennisetum purpureum) was selected; it presented the highest productivity (32 ton/ha/yr) and density (216 kg/m3) at harvesting age (mature material), highest glucan content, and lowest ash and uronic acid contents among the grasses evaluated.


## 3.1. Forestry Characteristics

Two very important factors regarding biomass use for pulp production are moisture content and density since they affect harvesting, transportation, and utilization costs. The eucalypt clones analyzed in this study show average moisture and density of 55% and 502 kg/m3. These values are considered satisfactory for pulp production [31–34]. The MAI varied in the range of 16–101.6 m3/ha/yr. The lowest MAI extreme occurred for the C1xC2 woody raw material. This may be explained due to the fact that eucalypt hybrid is poorly adapted to Minas Gerais State climate conditions and did not develop satisfactorily. Among the woody raw materials, the highest growth (101.6 m3/ha/yr) was obtained with sample DGxU2, which is a triple hybrid of (Eucalyptus dunnii xEucalyptus grandis) xEucalyptus urophylla (Table 2). This productivity is much above the average MAI obtained in commercial plantations in the Brazilian Territory (~40–60 m3/ha/yr) [5].Table 2
Forestry and physical characteristics of the eucalypt clones and elephant grass species evaluated.
Sample code
Moisture, %
Average annual increment, m3/ha/yr*
Biomass basic density, kg/m3
Biomass productivity, bone dry ton/ha/yr
Chip bulk density, kg/m3
U1xU2
54
86.0
504
43.3
209
U2xC1
55
54.1
547
29.6
220
G1xUGL
53
46.2
500
23.1
202
U1xUGL
54
46.9
496
23.3
193
U1xC2
53
52.9
517
27.4
203
C1xC2
54
16.0
533
8.5
207
DGxUGL1
55
57.7
449
25.9
193
DGxU2
56
101.6
496
50.4
203
C1xUGL
53
19.9
519
10.3
220
G1xGL2
54
39.3
530
20.8
211
DGxC1
53
72.6
500
36.3
213
U2xGL1
52
40.1
506
20.3
208
DGxGL2
55
28.5
489
13.9
197
U1xD2
54
42.6
441
18.8
178
U1xG2
56
63.4
518
32.8
228
IP
55
80.9
480
38.8
183
VC
54
80.3
473
38.0
179
CC
55
40.2
472
19.0
183
EG1
73
148.1
216
32.0
54
EG2
75
140.0
200
28.0
75
*Data provided by the feedstock suppliers.


## 3.2. Morphological Characteristics

Another important aspect of the raw material for pulp production is its morphological characteristics. The strength and morphology of fibers have a strong influence on the physical properties of paper as well as the wood pulpability [1, 2, 35]. In Table 3, the morphological characterizations of all the evaluated samples are presented.Table 3
Fiber and vessels characterization of the eucalypt clones and elephant grass species evaluated.
Samplecode
Fibre content, millions/g of pulp
Mean fibre arithmetic length,μm
Mean length-weighted fibre length,μm
Mean area-weighted length,μm
Mean fibre width,μm
Mean fibre coarseness, mg/m
Mean fibre curl index, %
Macrofibrillation index, %
Broken fibre content, %
Fine content, % in area
Mean fine area,μm2
Mean fine length,μm
Vessel content, nb/g of pulp
Mean area-weighted length, mm
Mean vessel width,μm
U1xU2
29.8
617.5
746.5
742.5
16.0
0.05
4.5
0.48
14.6
7.8
1707
65.5
8132
0.41
186.5
U2xC1
26.2
692.5
843.5
847.0
15.2
0.05
4.3
0.42
14.1
7.2
1666
64.5
3730
0.31
181.2
G1xUGL
27.9
635.0
779.5
775.0
16.5
0.05
5.0
0.45
15.0
8.0
1661
66.0
10795
0.44
185.0
U1xUGL
26.5
637.0
788.0
786.5
17.0
0.06
4.0
0.43
16.0
9.0
1590
64.0
3699
0.45
189.0
U1xC2
32.1
579.0
732.0
729.5
16.1
0.05
5.0
0.55
16.0
11.0
1537
63.0
3523
0.33
185.0
C1xC2
33.5
601.0
753.0
749.0
15.2
0.04
5.5
0.51
14.6
7.5
1594
64.0
3232
0.41
182.0
DGxUGL1
36.6
584.0
729.0
731.5
18.8
0.04
5.0
0.56
18.4
8.8
1512
61.0
1580
0.34
178.1
DGxU2
31.6
622.5
763.0
768.0
17.6
0.05
4.2
0.49
16.4
8.0
1574
61.5
1774
0.32
182.8
C1xUGL
35.8
631.5
791.0
789.5
15.0
0.04
6.1
0.50
14.3
6.8
1499
61.5
2756
0.30
181.4
G1xGL2
30.5
621.0
755.0
752.0
15.9
0.05
4.9
0.47
14.7
8.0
1534
61.5
3502
0.36
188.7
DGxC1
26.1
655.0
790.5
789.5
16.1
0.05
4.4
0.39
15.3
8.0
1663
63.0
7857
0.35
188.1
U2xGL1
37.5
589.0
717.5
719.5
15.7
0.04
5.4
0.58
15.0
7.4
1479
60.5
2891
0.43
183.5
DGxGL2
29.4
567.0
703.0
705.5
18.2
0.06
5.0
0.53
19.4
12.2
1462
58.5
2942
0.37
182.7
U1xD2
31.2
591.5
736.0
740.5
18.8
0.05
4.4
0.54
18.4
9.0
1469
59.0
8484
0.44
183.3
U1xG2
27.8
629.5
766.5
767.0
17.3
0.05
4.4
0.48
15.3
8.9
1751
64.5
10434
0.35
189.3
IP
28.1
562.0
733.0
733.5
16.4
0.06
4.5
0.54
17.7
12.4
1647
64.5
6771
0.43
194.3
VC
30.0
579.0
729.5
733.0
17.3
0.05
4.6
0.52
17.9
9.0
1578
63.5
3101
0.47
186.6
CC
33.8
551.5
692.5
695.5
18.3
0.05
5.1
0.57
18.1
11.6
1633
64.5
7546
0.45
190.2
EG1
14.9
695.5
1131
1114
21.5
0.09
6.8
0.42
26.1
11.9
1987
62
11628
0.45
191.1
EG2
21.7
686.0
1113
1059
19.1
0.06
6.0
0.45
20.6
11.1
2748
73
12023
0.61
205.6It was observed that the fiber content depends on the coarseness; the lower coarseness, the higher fiber content. The fiber coarseness is lower for samples U2xGL1, C1xC2, DGxUGL1, and C1xUGL and higher for samples DGxGL2, IP, and U1xUGL. This fiber coarseness measurement gives indication on the number of fibers required to reach a given basic weight and therefore affects the runnability of the paper machines, for example. The longest mean area-weighted fiber length is obtained for hybrid eucalypt clone U2xC1, followed by samples DGxC1, UG, DGxU2, G1xUGL, U1xUGL, and C1xUGL. The eucalypt clones DGxGL2 and CC have the shortest fiber. The length of the fiber has an influence on the mechanical properties of the final paper [36]. Longer fiber improves mechanical properties of the final paper. Curl index is higher for samples UxGL1, CC, C1xC2, and C1xUGL. This characteristic indicates that the fibers of these samples are less flexible than those of other eucalypt clone fibers. Macrofibrillation index and broken fiber content give information on the state of the fiber before deconstruction pretreatment or pulping. The CC and DGxUGL1 clones presented more broken fiber and the corresponding fiber is more fibrillated than the other clones. The clones DGxGL2, IP, and CC contain a higher amount of broken fiber which can explain the higher amount of fines on these eucalypt clones. For the pulping process, the vessel elements are desirable, since the penetration of cooking liquors is easier. However, for the production of special kinds of paper, such as printing papers, they are considered undesirable, because the vessel on the surface of the paper sheet tends to be pulled thereby causing printing failures; this is known as “vessels picking” [37]. Eucalypt clones DGxC1, U1xU2, U1xD2, U1xG2, G1xUGL, and CC have higher vessels content than the other samples. These vessels' content can be correlated to the growth rate of the tree, as, for example, the higher wood production, the higher vessels content. This means that, for the choice of the best clones to proceed with the study, the length of the fiber and the vessels content are the most important characteristics to help in the choice of the clones that should be used for the cooking process. Hybrid eucalypt clones C1xUGL, U1xUGL, and DGxUGL1 seem interesting because some characteristics, such as vessels content, fiber length, broken fiber, and fines content, are adequate for quality of papers.Regarding elephant grass samples, a lower fiber content per gram in EG1 and EG2 samples was observed. The width, length, coarseness, fine content, and macrofibrillation index may be considered close to the eucalypt samples. However, the EG1 and EG2 showed a high vessel content. Vessels are recognized to generate problems during printing as explained previously [37]. But in general it is possible to say that the fibers have potential for paper production, since the characteristics desired for paper quality are observed.


## 3.3. Chemical Characteristics

Table4 shows the extractive quantity of the eucalypt clones and elephant grass samples extracted with the ethanol/toluene → ethanol → hot water solvent series, with ethanol/toluene 1 : 2 only and with acetone. In order to measure the biomass cell wall components, it is relevant to remove all extractives present in the material. The Tappi T204 CM 97 standard procedure [24] (ethanol/toluene 1 : 2 → ethanol → hot water) was efficient for removing all polar and apolar extractive fractions. Although this procedure is intended to free the wood from extractives, it serves also to quantify the total amount of extractives present in the biomass, since the main cell wall components (cellulose, hemicelluloses, and lignin) are not soluble in any of the solvents comprising the series. Extraction with ethanol/toluene only extracts substances as waxes, fats, resins, phytosterols, and nonvolatile hydrocarbons. Extraction in acetone [23] serves to quantify those extractives that are more relevant to the pulping operation and pitch formation in the pulp. The acetone extractable content of wood is a measure of such substances as fatty acids, resin acids, sterols, waxes, and nonvolatile hydrocarbons. Because acetone is both more polar and water-miscible than dichloromethane or benzene-ethanol, the quantity of acetone extractable material, especially in wood, may be higher than that found with the other solvents. This procedure will not give the same results as ethanol-toluene or dichloromethane extractions. In their work, Barbosa et al. [38] showed that acetone is the best solvent for the evaluation of the wood lipophilic extract content.Table 4
Extractive content of the eucalypt clones and elephant grass species evaluated.
Sample code
Acetone extractives, %
Ethanol/toluene (1 : 2) extractives, %
Total extractives, %
U1xU2
1.7
1.9
3.6
U2xC1
1.1
1.4
3.4
G1xUGL
2.5
2.5
4.9
U1xUGL
1.9
1.3
3.6
U1xC2
1.2
1.2
2.8
C1xC2
1.2
1.3
3.0
DGxUGL1
1.2
2.7
2.8
DGxU2
1.2
2.2
2.7
C1xUGL
0.9
1.6
2.1
G1xGL2
1.2
1.9
2.8
DGxC1
0.9
1.2
1.9
U2xGL1
1.0
1.4
2.5
DGxGL2
1.1
1.7
1.9
U1xD2
2.3
2.3
3.0
U1xG2
2.1
1.6
2.8
IP
0.8
1.5
2.3
VC
1.6
2.6
3.7
CC
1.1
2.6
3.5
EG1
3.9
6.8
14.8
EG2
9.9
1.8
17.6Biomass extractives are quite troublesome since they cause many difficulties in operating the industrial facilities, causing unexpected lost time in the operation for cleaning of equipment and instruments due to their stickiness and tackiness. In addition the deposition of these substances in the pulp may occur, which are called pitch [38], decreasing the pulp value or even its rejection by market. In their study Cruz et al. [39] showed that in pitch composition there are waxes, fats, and long chain alcohols, being the main compounds associated with pitch formation [40].For the pulp production, among the eucalypt clones acceptable total extractive contents (1.9 to 4.9%) were observed for all clones [34, 41]. On the other hand, the EG samples showed a high content of acetone extractives and ethanol/toluene extractives. In addition, pitch formation and pulp dirtiness are much more likely on raw materials containing high content of these extractives. As a consequence, the EG1 and EG2 also presented a high total extractive content (14.8% and 17.6, resp.) since such materials are likely to result in low yield during cooking process.The raw material minerals are detrimental for their industrial utilization, since they cause corrosion and deposits on equipment, reduce biomass heating value, and decrease mill throughput. In Table5 the results of biomass mineral content are presented. In general the amounts of inorganics present in the eucalypt clones were very low and quite acceptable for most applications [42, 43]. For the eucalypt clones, the total inorganics measured by complete biomass combustion (ash content) varied in the range of 950–2,510 mg/kg biomass. For the EG1 and EG2, they reached 60, 100, and 37,900 mg/kg, respectively. The very high mineral content in the grass is explained by its fast metabolism at the young age when it needs plenty of minerals to produce the biomass. Another interesting fact about the contents of minerals in biomass is that they tend to decrease with aging due to decreased biomass deposition rate as a function of time [44]. The same trend observed for total inorganics (ash content) is also verified for the individual components such as silica, chloride, calcium, potassium, and magnesium, with the elephant grass samples always presenting higher values than the eucalypt clone samples. Calcium, magnesium, and silica are very undesirable in most industrial processes because of their ability to cause deposits in equipment during evaporation of liquid streams and combustion of solid streams. On the other hand, potassium and chloride are particularly dangerous for their ability to decrease the ash melting point during combustion, thus causing sticky ash problems in recovery boiler systems [45]. In addition, chlorides are highly corrosive and troublesome for most equipment regardless of metallurgy. In regard to transition metals (Fe, Cu, and Mn), there were also significant differences among the eucalypt clones and elephant grass materials, again always presenting the same trend, higher content in elephant grass samples.Table 5
Ash and metal content of the eucalypt clones evaluated.
Sample code
Inorganics, mg/kg biomass
Ash
Cu
Fe
Ca
Mn
Mg
K
Cl−
U1xU2
950.0
0.8
15.5
307.0
9.5
81.0
194.0
260.0
U2xC1
1850.0
0.7
12.3
384.0
16.0
146.0
265.0
328.0
G1xUGL
1950.0
0.5
13.1
452.0
14.0
112.0
188.0
629.0
U1xUGL
1200.0
1.2
14.6
394.0
9.8
148.0
185.0
454.0
U1xC2
1250.0
0.7
10.3
328.0
9.7
144.0
340.0
594.0
C1xC2
2510.0
1.1
8.2
518.0
19.8
156.0
404.0
413.0
DGxUGL1
1950.0
0.8
18.2
532.0
16.3
118.0
358.0
500.0
DGxU2
2280.0
1.2
13.5
531.0
13.2
123.0
218.0
427.0
C1xUGL
2160.0
0.6
10.6
627.0
23.6
166.0
435.0
523.0
G1xGL2
1790.0
0.6
19.5
525.0
18.9
129.0
450.0
446.0
DGxC1
1400.0
0.9
10.6
263.0
11.2
128.0
252.0
701.0
U2xGL1
1800.0
0.7
13.5
475.0
14.3
136.0
152.0
519.0
DGxGL2
1750.0
1.0
11.3
462.0
15.0
123.0
202.0
600.0
U1xD2
1400.0
0.8
14.8
264.0
10.9
115.0
222.0
689.0
U1xG2
1850.0
1.1
20.4
296.0
11.2
92.0
384.0
477.0
IP
1550.0
1.1
9.3
378.0
18.2
104.0
369.0
434.0
VC
1800.0
1.4
9.9
491.0
2.2
123.0
469.0
488.0
CC
1700.0
1.2
8.5
356.0
13.9
183.0
370.0
399.0
EG1
60100.0
8.8
11.2
423
11.1
490.0
21194
15167.0
EG2
37900.0
3.8
67.5
352
56.5
1201.0
16055
2335.0In Table6 the biomass chemical composition is presented. There were significant variations among the results of contents of sugars, acetyl group, uronic acids, lignin, and syringyl/guaiacyl ratio of lignin. Among the eucalypt clones, the total lignin contents varied in the range of 27.1 to 31.3%. The maximum value was obtained for the double crossing U1xC2 hybrid and the minimum for the G1xGL2 one. However, these values are considered acceptable for eucalypt clones, but for pulp production a lower lignin content and high S/G ratio are desired due to the increase of the pulpability of the wood [46]. About the carbohydrate content, the eucalypt clones presented values considered satisfactory for eucalypts aiming at pulp production [34].Table 6
Chemical composition of the eucalypt clones and elephant grass species evaluated.
Sample code
Sugar composition, %
Acid soluble lignin %
Total lignin %
Lignin S/G ratio
Acetyl group %
Uronic acid group   %
Glucan
Xylans
Galactans
Mannans
Arabinans
U1xU2
46.1
11.8
0.8
0.8
0.2
4.3
30.3
2.8
2.1
3.7
U2xC1
45.5
10.7
1.2
1.0
0.2
4.6
30.8
2.7
1.9
4.0
G1xUGL
43.9
13
0.8
0.9
0.2
4.7
28.9
2.9
2.7
3.8
U1xUGL
44.9
11.7
1.1
0.8
0.2
5
29.7
3.1
2.4
4.0
U1xC2
45.6
10
1.4
1.1
0.2
4.5
31.3
3.0
1.8
3.8
C1xC2
45.6
9.7
1.6
0.9
0.3
5.1
31.1
3.0
1.6
4.1
DGxUGL1
45.5
13
0.9
0.8
0.3
5.3
29.2
3.2
2.6
4.0
DGxU2
45.3
12.6
0.9
1.0
0.3
4.4
29.8
2.6
2.5
4.0
C1xUGL
46.0
11.9
1.1
0.9
0.3
5.0
30.7
3.2
2.2
4.0
G1xGL2
46.4
14.1
1.0
0.8
0.3
5.0
27.1
3.5
3.0
3.9
DGxC1
47.2
10.8
1.2
0.9
0.3
4.7
31.0
2.8
1.8
4.0
U2xGL1
45.5
13.4
1.2
0.8
0.3
5.6
29.3
3.8
2.6
4.0
DGxGL2
45.8
12.9
1.1
1.0
0.3
4.4
28.2
2.9
2.5
3.8
U1xD2
48.1
11.4
1.0
0.9
0.3
4.4
28.6
2.6
2.0
3.9
U1xG2
46.8
11.8
1.0
0.8
0.3
4.4
30.2
2.6
2.0
4.0
IP
49.4
12
1.2
0.9
0.3
4.2
27.2
2.7
1.9
4.0
VC
46.9
11.4
0.8
1.1
0.2
4.6
28.4
2.9
2.1
3.8
CC
47.4
11.2
1.0
1.1
0.2
4.1
28.4
2.4
1.9
3.9
EG1
38.2
9.6
0.8
0.6
0.2
2.2
18.0
0.8
1.9
1.2
EG2
35.6
16.1
0.5
ND
1.6
1.6
16.5
0.7
1.8
1.4Regarding elephant grass, the lignin contents for EG1 and EG2 biomass were 18 and 16.5%, respectively. The low lignin content of the elephant grass samples is advantageous for potentially improving pulping easiness and yield. However, its low lignin S/G ratio and low sugar content work in the opposite direction [46]. The low sugar content of the elephant grass samples reflected their very high extractive and mineral contents.


## 3.4. Some Relations among Cell Wall Components

In order to determine lignin content, it is necessary to treat biomass with strong acids so that the carbohydrate fraction is hydrolyzed, leaving lignin as a residue. This acid hydrolysis procedure leads to severe lignin condensation and precipitation so that it is easily collected from the solution through simple filtration. Some of the lignin, particularly in hardwoods, seemingly condenses insufficiently in the acid media, becomes soluble, and sieves through the filtration system. This lignin is the so-called acid soluble lignin. Although acid soluble lignin is negligible for softwood biomass, it is quite important for hardwoods. The amount of acid soluble lignin in the woody biomass investigated varied in the range of 4.1–5.6% of the wood dry weight (Table5). Although not commonly investigated, the amount of acid soluble lignin seems to be related to the lignin S/G ratio. Lignin containing larger amounts of syringyl monomers will condense less during the strong acid hydrolysis treatment, since the C5 position in the aromatic ring is blocked in the syringyl units. These blocked C5 positions prevent C5 condensation. Therefore, lignin containing high S/G ratio, that is, high number of syringyl units, will condense less during the acid hydrolysis procedure and produce more soluble lignin in the filtrates. Figure 1 shows that there is very good correlation between lignin S/G ratio and acid soluble lignin content for the various woody and nonwoody biomass, in agreement with the proposed theory.Figure 1
Correlation between biomass acid soluble lignin and lignin S/G ratio.


## 3.5. Raw Material Selection

Based on all the analyses, the best five eucalypt clones and one elephant grass selected for the pulp production were(1)
a double crossing hybrid ofEucalyptus urophylla xEucalyptus urophylla (U1xU2), that was selected on the basis of its very high annual growth (83 m3/ha/yr), high wood density, excellent morphological traits, very high forest yield (43 ton/ha/yr), and low xylan and uronic acid contents;(2)
a triple crossing hybrid ofEucalyptus grandis x (Eucalyptus urophylla xEucalyptus globulus) (G1xUGL), which presented a high xylan content and possessedEucalyptus globulus in its genotype, which is of interest for high S/G ratio, although it is quite challenging for its high content of extractives (4.9%);(3)
a triple crossing of (Eucalyptus dunnii xEucalyptus grandis) xEucalyptus urophylla (DGxU2), that was selected due to its highest annual growth (101 m3/ha/yr) among all eucalypts evaluated, good density, outstanding morphological traits, and the highest forest yield (~50 ton/ha/yr);(4)
a commercial elite clone (IP) that was obtained from a large Brazilian forest company, which is a double crossing ofEucalyptus urophylla xEucalyptus grandis, for its excellent forest productivity (38.5 ton/ha/yr), good density, the highest cellulose content, and lowest lignin content among all and also for being a very good reference since it is commercially planted by a large pulp company in Brazil;(5)
regarding the elephant grass, the EG1 (Pennisetum purpureum) was selected; it presented the highest productivity (32 ton/ha/yr) and density (216 kg/m3) at harvesting age (mature material), highest glucan content, and lowest ash and uronic acid contents among the grasses evaluated.


## 4. Conclusions

The results of this study indicate the high technological quality ofEucalyptus clones evaluated and indicate that they can be used for pulp production since they have the suitable characteristics. In general, the eucalypt clones are less moist and denser and contain fewer minerals and extraneous materials than the elephant grass species, which make them more attractive for utilization in deconstruction studies aiming at production of bioproducts.



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*Source: 814071-2015-02-23.xml*