# Glufosinate Rate and Timing for Control of Glyphosate-Resistant Rhizomatous Johnsongrass (Sorghum halepense) in Glufosinate-Resistant Soybean

Authors: Randall L. Landry; Daniel O. Stephenson; Brandi C. Woolam
Journal: International Journal of Agronomy
 (2016)

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## Abstract

Field studies were conducted over a three-year period (2011, 2012, and 2013) in Louisiana to evaluate the effect of glufosinate rate and timing on glyphosate-resistant (GR) rhizomatous johnsongrass control in glufosinate-resistant soybean. Treatments included glufosinate (0.5, 0.6, or 0.7 kg ai ha−1) applied alone POST1 (46 cm tall johnsongrass) and sequentially 3 (POST2) or 4 (POST3) wk after POST1 at 0.5 or 0.6 kg ha−1. Glufosinate (0.7 kg ha−1) applied POST1 controlled johnsongrass 77% at soybean harvest. Averaged across sequential application rate, delaying the sequential application from POST2 to POST3 increased control from 65 to 78% at harvest. Increasing sequential application rate from 0.5 to 0.6 kg ha−1 reduced johnsongrass heights 15% at harvest. Furthermore, delaying the sequential application from POST2 to POST3 reduced GR rhizomatous johnsongrass heights to 63% of the nontreated at harvest. Soybean yields were maximized following the POST1 application of glufosinate at 0.7 kg ha−1 (2670 kg ha−1) and by applying 0.6 kg ha−1 of glufosinate sequentially (2620 kg ha−1), regardless of sequential application timing. Maximum control and soybean yield were observed following glufosinate POST1 at 0.7 kg ha−1 followed by 0.6 kg ha−1 at POST3. This data indicates that glufosinate is an option for management of GR rhizomatous johnsongrass.


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## Body

## 1. Introduction

Louisiana growers planted 457,000, 570,000, and 579,000 ha of soybean [Glycine max (L.) Merr.] in 2013, 2014, and 2015, respectively [1]. Weed interference in soybean can cause yield losses that range from 5 to 100%, depending upon weed species and species density [2–6]. Johnsongrass infestations compete with soybean for light, moisture, and nutrients, which extends time needed for seed drying and slows mechanical harvest [7]. In addition, johnsongrass infestations in soybean can reduce yields up to 42% [7] and can serve as a host for insects and diseases [8].Johnsongrass is native to the Mediterranean region [9, 10] and its range has been expanded by environmental (i.e., animals, wind, and water), human (i.e., sowing as forage), and mechanical means (i.e., harvesters, cultivators). The current range of johnsongrass is from latitude 55° N to latitude 45° S and it is found on six continents; the only continent where it is not found is Antarctica [9]. Expansion to colder climates is not expected because johnsongrass rhizomes are not tolerant of freezing temperatures [11]. Information is limited on introduction into North America; however, it is reported that johnsongrass was planted extensively as a forage in the 1830s [12]. By 1975, johnsongrass was reported in 59 of the 64 parishes in Louisiana [13].Early johnsongrass control in soybean relied on combinations of cultural and mechanical methods [14, 15]. McWhorter [16, 17] reported acceptable johnsongrass control after two successive years of soil-incorporated dinitroaniline herbicides. The acetyl coenzyme-A carboxylase- (ACCase-) inhibitors, which were introduced in the 1980s, provided soybean producers with a POST option for johnsongrass control. Sequential POST applications of sethoxydim, fluazifop-P, haloxyfop, quizalofop, or clethodim provided 70 to 90% control of johnsongrass [18]. Similarly, Winton-Daniels et al. [19] reported greater johnsongrass control following sequential POST applications of fenoxaprop, fluazifop-p, haloxyfop, quizalofop, and sethoxydim than a single application.The commercialization of glyphosate-resistant (GR) soybean in the 1990s provided producers another POST option to effectively manage many broadleaf and grass weeds [20–23], including rhizomatous johnsongrass. In GR soybean, glyphosate controlled johnsongrass greater than 90% 50 days after treatment (DAT) [24, 25]. As a consequence of glyphosate’s excellent activity on johnsongrass and numerous other broadleaf and grassy weeds, 92% of all GR soybean acres were treated with glyphosate in 2006 in the United States [26]. However, Green and Owen [27] reported that excessive use of a single herbicide mode of action over time could result in the selection of weed biotypes that are resistant to that herbicide. The overreliance on glyphosate for weed management leads to the development of glyphosate-resistant weeds with 32 species documented globally [28]. Furthermore, GR johnsongrass has been reported in Argentina, Arkansas, Mississippi, and Louisiana [28–31]. With continued reliance on glyphosate for weed management in GR crops, it is imperative that integrated weed management strategies are developed [32, 33], specifically for GR rhizomatous johnsongrass.Introduction of glufosinate-resistant soybean in 1996 provided growers the opportunity to apply glufosinate POST to weeds without injury to the soybean [34]. Glufosinate interrupts essential amino acid biosynthesis by inhibiting the glutamine synthetase enzyme, which is responsible for converting glutamate and ammonia to glutamine [35]. This inhibition causes a buildup of ammonia in susceptible plants which rapidly destroys cells and tissue [36, 37]. Glufosinate provides broad-spectrum control of many grass and broadleaf weeds [38, 39]. At 14 DAT, Johnson and Norsworthy [40] observed an inverse relationship between johnsongrass height at glufosinate (0.7 kg ha−1) application and control with 96, 91, and 78% control of 15, 30, and 45 cm johnsongrass, respectively. However, effective johnsongrass control has been reported with sequential applications of glufosinate or herbicide combinations with glufosinate (80% or greater 10 WAE), but sequential applications were made at the same rate (e.g., 590 g ai ha−1 followed by (fb) 590 g ai ha−1) and timing (3 and 6 wk after emergence) [41].Johnsongrass populations resistant to both glyphosate and ACCase-inhibitors have not been discovered in Louisiana. However, current utilization of only ACCase-inhibiting herbicides to control GR rhizomatous johnsongrass populations could potentially lead to populations with multiple resistance to glyphosate and ACCase-inhibitors. Alternative strategies need to be investigated for management of current GR rhizomatous johnsongrass populations. However, limited research is available pertaining to glufosinate rates and application timings for control of GR rhizomatous johnsongrass in glufosinate-resistant soybean. Therefore, the objective of this research was to evaluate glufosinate rates and application timings for control of GR rhizomatous johnsongrass.


## 2. Materials and Methods

Three field studies were conducted in 2011, 2012, and 2013 at Louisiana State University Agricultural Center Dean Lee Research and Extension Center near Alexandria, LA. Soil was a Coushatta silt loam (fine-silty, mixed, superactive, thermic Fluventic Eutrudepts) with a pH of 8.0 and 1.5% organic matter. An augmented factorial arranged in a randomized complete block replicated four times was used in all studies. Treatments include an initial application (POST1) of glufosinate POST1 (Liberty 280 SL, herbicide, Bayer CropScience LP 2 T.W. Alexander Drive Research Triangle Park, North Carolina 27709) at 0.5, 0.6, or 0.7 kg ai ha−1 followed by a sequential application of glufosinate at 0.5 or 0.6 kg ha−1 3 (POST2) or 4 (POST3) wk after POST1, plus a nontreated control.Soybean planting in Louisiana typically begins in early-April; however, johnsongrass emergence can begin in early-March, reach heights of 10 to 20 cm by mid-April, and, oftentimes, reach 30 to 50 cm in height at V2 to V4 soybean (Daniel O. Stephenson IV, personal communication). Therefore, glufosinate (0.5, 0.6, or 0.7 kg ha−1) was applied to 46 cm johnsongrass (POST1). Glufosinate was applied at POST2 or POST3 to 122 cm johnsongrass, but johnsongrass leaf number was greater at POST3 (5 versus 7 leaves). Corresponding soybean growth stages at the POST1 and POST2/POST3 were VC to V4 (0.6 to 20 cm tall) and V6 to V8 (15 to 91 cm tall), respectively, in all years.Herbicides were applied with a tractor-mounted, compressed-air sprayer calibrated to deliver 187 L ha−1 at 145 kPa using Teejet 11002, flat fan nozzles (Spraying Systems Co., P.O. Box  7900, Wheaton, IL 60189). Plot size was 9 m long and 3.9 m wide with four soybean rows spaced 0.97 m apart. “Merschman Miami 949,” “Halo 494,” and “HBK 4950” glufosinate-resistant soybean were planted in 2011, 2012, and 2013, respectively. Soybean was seeded at 282,400 seeds ha−1 in 2011 and 305,900 seeds ha−1 in 2012 and 2013. All studies were conducted using conventional-tillage methods and standard soybean production practices.Soybean injury, johnsongrass control (0% no control to 100% complete control), and heights were assessed 20 and 28 DAT and at harvest. Johnsongrass heights were determined by measuring five plants per plot from the soil surface to the bend of the upper most leaf. Prior to analysis, johnsongrass heights were converted to a percentage of the nontreated control. Yield was determined by harvesting the center two rows using conventional harvesting equipment. Johnsongrass densities in the nontreated averaged 200 plants m−2 prohibiting machine harvest of these plots; thus the nontreated yields were excluded from analysis. Yield was adjusted to 13% moisture before analysis.All data were subjected to analysis of variance using PROC GLIMMIX in SAS (release 9.3, SAS Institute, Cary, NC). Type III statistics were used to test all possible fixed effects (initial and sequential glufosinate application rates and sequential glufosinate application timing) or interactions among the fixed effects. Random effects were years and replications nested within in years [42]. Considering year a random effect permits inferences about treatments to be made over a range of environments [42, 43]. Table 1 provides P values of all main effects and interactions from analysis. Least square means were calculated and means separated (P
≤
0.05) using Tukey’s honest significant difference test. All data were subject to arcsine square root transformation to test for normality [44], but nontransformed means are presented.Table 1
Significance of the main effects of initial glufosinate application rate (POST1), sequential glufosinate application rate, and sequential glufosinate application timing (POST2 or POST3) and interactions among main effects pooled across environments 20 and 28 d after treatment (DAT) and at harvest for glyphosate-resistant rhizomatous johnsongrass control and height as a percent of the nontreated and soybean grain yield.
Parameter
Data collection
IRa
SR
ST
IT × SR
IT × ST
SR × ST
IT × SR × ST
P valueb
Control
20 DAT
0.0002
0.3073
0.2284
0.1628
0.0853
0.4139
0.6861
28 DAT
0.0541
0.0575
0.0094
0.2399
0.4606
0.3473
0.6594
At harvest
0.0107
0.5275
0.0014
0.2678
0.7232
0.1330
0.4546
Height
20 DAT
0.0397
0.4905
0.0041
0.5300
0.3473
0.1703
0.0931
28 DAT
0.2998
0.0358
0.0253
0.1106
0.3439
0.7247
0.6855
At harvest
0.4725
0.0042
0.0191
0.8517
0.6954
0.9213
0.9310
Soybean grain yield
0.0076
0.0346
0.5869
0.2337
0.2896
0.7770
0.7820
aIR: initial glufosinate application rate (POST1); SR: sequential glufosinate application rate; ST: sequential glufosinate application timing (POST2 or POST3).
bMain effects and interactions considered significant if P
≤
0.05.


## 3. Results and Discussion

### 3.1. Soybean Injury

Glufosinate applied POST (0.5 to 0.7 kg ha−1) did not cause any injury to glufosinate-resistant soybean at any evaluation date (data not shown). Pline et al. [45] reported similar results 6 and 23 DAT. In contrast, Beyers et al. [46] observed ≤11% injury 2 WAT, but injury was ≤1% 4 WAT. Results from numerous studies conclude that there is an adequate margin of crop safety with glufosinate applied POST in glufosinate-resistant soybean.


### 3.2. Glyphosate-Resistant Rhizomatous Johnsongrass Control

Control 20 DAT was greater following glufosinate POST1 at 0.7 kg ha−1 (85%) and 0.6 kg ha−1 (83%) compared with 0.5 kg ha−1 (79%) (Table 2). Furthermore, greater GR rhizomatous johnsongrass control was observed at harvest following the POST1 application of 0.7 kg ha−1 (77%) than 0.5 kg ha−1 (64%), while control following application of 0.5 and 0.7 kg ha−1 was similar to 0.6 kg ha−1 (74%) (Table 2). In contrast, Johnson and Norsworthy [40] observed no difference in control between 0.5, 0.6, and 0.7 kg ha−1 of glufosinate when applied to 45 cm johnsongrass with an average of 75 and 62% control 14 and 28 DAT, respectively. The increased control observed in this research may be due to higher relative humidity prior to, at, and following glufosinate applications. Glufosinate activity on common waterhemp (Amaranthus rudis Sauer), green foxtail [Setaria viridis (L.) Beauv.], Palmer amaranth (Amaranthus palmeri S. Wats.), redroot pigweed (Amaranthus retroflexus L.), and wild oat (Avena fatua L.) was greater following application when relative humidity was 90% or greater [47–49] with the relative humidity within 12 h of glufosinate application having the greatest effect on activity [49]. Maximum daily relative humidity following all POST applications was 92 to 94% one day prior, day of, and after application in each year (data not shown). Therefore, high relative humidity may be the reason for greater johnsongrass control observed in this study compared with that of Johnson and Norsworthy [40]. Others [25, 40, 50] also observed an increase in johnsongrass control following increased rates of glufosinate.Table 2
Glyphosate-resistant rhizomatous johnsongrass control 20 d after treatment (DAT) and at harvest and heights as a percent of the nontreated 20 DAT and soybean yield as influenced by POST1 glufosinate application ratea.
Rate
Johnsongrass control
Johnsongrass heights
Soybean yield
kg ai ha−1
20 DATb (%)
At harvest (%)
20 DAT (%)
kg ha−1
0.5
79b
64b
48a
2400b
0.6
83a
74ab
42ab
2600a
b
0.7
85a
77a
41b
2670a
aData pooled over sequential glufosinate application rate of 0.5 or 0.6 kg ai ha−1 and POST2 or POST3 sequential glufosinate application timing.
bMeans followed by the same letter within each column are not significantly different based on Tukey’s HSD at P
≤
0.05.Although no difference in johnsongrass control was observed 20 DAT between POST2 and POST3 (P
=
0.2284), delaying the sequential application from POST2 to POST3 increased johnsongrass control 7 and 13% 28 DAT and at harvest, respectively (Table 3). This observation may be due to increased johnsongrass leaf number (5 leaves versus 7 leaves) providing greater interception of glufosinate when applied POST3. Johnson et al. [41], Wiesbrook et al. [50], and Prostko et al. [51] suggested that sequential applications of herbicides are more effective at controlling weeds.Table 3
Glyphosate-resistant rhizomatous johnsongrass control 28 d after treatment (DAT) and at harvest and heights as a percent of the nontreated 20 and 28 DAT and at harvest as influenced by POST2 or POST3 sequential glufosinate application timinga.
Timing
Johnsongrass control
Johnsongrass heights
28 DATb (%)
At harvest (%)
20 DAT (%)
28 DAT (%)
At harvest (%)
POST2
76b
65b
47a
49a
76a
POST3
83a
78a
40b
36b
63b
aData pooled over POST1 glufosinate application rate of 0.5, 0.6, or 0.7 kg ai ha−1 and POST2 and POST3 sequential glufosinate application rate of 0.5 or 0.6 kg ai ha−1.
bMeans followed by the same letter within each column are not significantly different based on Tukey’s HSD at P
≤
0.05.


### 3.3. Glyphosate-Resistant Rhizomatous Johnsongrass Heights

At 20 DAT, glufosinate applied at 0.5, 0.6, and 0.7 kg ha−1 POST1 reduced johnsongrass heights 48, 42, and 41% of the nontreated control, respectively, with differences between 0.5 and 0.7 kg ha−1 (Table 2), but these differences were not observed 28 DAT (P
=
0.2998) or at harvest (P
=
0.4725). Conversely, the sequential glufosinate application rate did not influence GR rhizomatous johnsongrass heights as a percent of the nontreated 20 DAT (P
=
0.4905), but increasing the sequential glufosinate application rate from 0.5 to 0.6 kg ha−1 decreased GR rhizomatous johnsongrass heights 11 and 15% 28 DAT and at harvest, respectively (Table 4). Additionally, delaying the sequential glufosinate application timing from POST2 to POST3 decreased GR rhizomatous johnsongrass heights as a percent of the nontreated 7 to 13% at all evaluation dates (Table 3). Johnson and Norsworthy [40] reported johnsongrass stand reductions following applications of glufosinate (65 to 73%), but no differences were detected between rates 28 DAT.Table 4
Glyphosate-resistant rhizomatous johnsongrass heights as a percent of the nontreated 28 d after treatment (DAT) and at harvest and soybean yield as influenced by POST2 and POST3 sequential glufosinate application rate.
Rate
Johnsongrass heights
Soybean yield
kg ai ha−1
28 DAT (%)
At harvest (%)
kg ha−1
0.5
48a
77a
2490b
0.6
37b
62b
2620a
aData pooled over POST1 glufosinate application rate of 0.5, 0.6, or 0.7 kg ai ha−1 and POST2 and POST3 sequential glufosinate application timing.
bMeans followed by the same letter within each column are not significantly different based on Tukey’s HSD at P
≤
0.05.


### 3.4. Soybean Yield

Soybean yields were 2400, 2600, and 2670 kg ha−1 following POST1 at 0.5, 0.6, and 0.7 kg ha−1, respectively, but only yields following the 0.5 and 0.7 kg ha−1 rates differed (Table 2). Similarly, increasing the sequential glufosinate application rate from 0.5 to 0.6 kg ha−1 increased soybean yield 130 kg ha−1 (Table 4). No difference was observed between sequential glufosinate application timings (P
=
0.5869) with soybean yielding 2440 and 2550 kg ha−1 following POST2 and POST3, respectively (data not shown). Conversely, Johnson et al. [41] observed no differences in glufosinate-resistant soybean yield following herbicide programs for johnsongrass control (2,690 to 3,160 kg ha−1); however, differences might have been observed if their studies had not been sprayed with clethodim 10 WAE.


## 3.1. Soybean Injury

Glufosinate applied POST (0.5 to 0.7 kg ha−1) did not cause any injury to glufosinate-resistant soybean at any evaluation date (data not shown). Pline et al. [45] reported similar results 6 and 23 DAT. In contrast, Beyers et al. [46] observed ≤11% injury 2 WAT, but injury was ≤1% 4 WAT. Results from numerous studies conclude that there is an adequate margin of crop safety with glufosinate applied POST in glufosinate-resistant soybean.


## 3.2. Glyphosate-Resistant Rhizomatous Johnsongrass Control

Control 20 DAT was greater following glufosinate POST1 at 0.7 kg ha−1 (85%) and 0.6 kg ha−1 (83%) compared with 0.5 kg ha−1 (79%) (Table 2). Furthermore, greater GR rhizomatous johnsongrass control was observed at harvest following the POST1 application of 0.7 kg ha−1 (77%) than 0.5 kg ha−1 (64%), while control following application of 0.5 and 0.7 kg ha−1 was similar to 0.6 kg ha−1 (74%) (Table 2). In contrast, Johnson and Norsworthy [40] observed no difference in control between 0.5, 0.6, and 0.7 kg ha−1 of glufosinate when applied to 45 cm johnsongrass with an average of 75 and 62% control 14 and 28 DAT, respectively. The increased control observed in this research may be due to higher relative humidity prior to, at, and following glufosinate applications. Glufosinate activity on common waterhemp (Amaranthus rudis Sauer), green foxtail [Setaria viridis (L.) Beauv.], Palmer amaranth (Amaranthus palmeri S. Wats.), redroot pigweed (Amaranthus retroflexus L.), and wild oat (Avena fatua L.) was greater following application when relative humidity was 90% or greater [47–49] with the relative humidity within 12 h of glufosinate application having the greatest effect on activity [49]. Maximum daily relative humidity following all POST applications was 92 to 94% one day prior, day of, and after application in each year (data not shown). Therefore, high relative humidity may be the reason for greater johnsongrass control observed in this study compared with that of Johnson and Norsworthy [40]. Others [25, 40, 50] also observed an increase in johnsongrass control following increased rates of glufosinate.Table 2
Glyphosate-resistant rhizomatous johnsongrass control 20 d after treatment (DAT) and at harvest and heights as a percent of the nontreated 20 DAT and soybean yield as influenced by POST1 glufosinate application ratea.
Rate
Johnsongrass control
Johnsongrass heights
Soybean yield
kg ai ha−1
20 DATb (%)
At harvest (%)
20 DAT (%)
kg ha−1
0.5
79b
64b
48a
2400b
0.6
83a
74ab
42ab
2600a
b
0.7
85a
77a
41b
2670a
aData pooled over sequential glufosinate application rate of 0.5 or 0.6 kg ai ha−1 and POST2 or POST3 sequential glufosinate application timing.
bMeans followed by the same letter within each column are not significantly different based on Tukey’s HSD at P
≤
0.05.Although no difference in johnsongrass control was observed 20 DAT between POST2 and POST3 (P
=
0.2284), delaying the sequential application from POST2 to POST3 increased johnsongrass control 7 and 13% 28 DAT and at harvest, respectively (Table 3). This observation may be due to increased johnsongrass leaf number (5 leaves versus 7 leaves) providing greater interception of glufosinate when applied POST3. Johnson et al. [41], Wiesbrook et al. [50], and Prostko et al. [51] suggested that sequential applications of herbicides are more effective at controlling weeds.Table 3
Glyphosate-resistant rhizomatous johnsongrass control 28 d after treatment (DAT) and at harvest and heights as a percent of the nontreated 20 and 28 DAT and at harvest as influenced by POST2 or POST3 sequential glufosinate application timinga.
Timing
Johnsongrass control
Johnsongrass heights
28 DATb (%)
At harvest (%)
20 DAT (%)
28 DAT (%)
At harvest (%)
POST2
76b
65b
47a
49a
76a
POST3
83a
78a
40b
36b
63b
aData pooled over POST1 glufosinate application rate of 0.5, 0.6, or 0.7 kg ai ha−1 and POST2 and POST3 sequential glufosinate application rate of 0.5 or 0.6 kg ai ha−1.
bMeans followed by the same letter within each column are not significantly different based on Tukey’s HSD at P
≤
0.05.


## 3.3. Glyphosate-Resistant Rhizomatous Johnsongrass Heights

At 20 DAT, glufosinate applied at 0.5, 0.6, and 0.7 kg ha−1 POST1 reduced johnsongrass heights 48, 42, and 41% of the nontreated control, respectively, with differences between 0.5 and 0.7 kg ha−1 (Table 2), but these differences were not observed 28 DAT (P
=
0.2998) or at harvest (P
=
0.4725). Conversely, the sequential glufosinate application rate did not influence GR rhizomatous johnsongrass heights as a percent of the nontreated 20 DAT (P
=
0.4905), but increasing the sequential glufosinate application rate from 0.5 to 0.6 kg ha−1 decreased GR rhizomatous johnsongrass heights 11 and 15% 28 DAT and at harvest, respectively (Table 4). Additionally, delaying the sequential glufosinate application timing from POST2 to POST3 decreased GR rhizomatous johnsongrass heights as a percent of the nontreated 7 to 13% at all evaluation dates (Table 3). Johnson and Norsworthy [40] reported johnsongrass stand reductions following applications of glufosinate (65 to 73%), but no differences were detected between rates 28 DAT.Table 4
Glyphosate-resistant rhizomatous johnsongrass heights as a percent of the nontreated 28 d after treatment (DAT) and at harvest and soybean yield as influenced by POST2 and POST3 sequential glufosinate application rate.
Rate
Johnsongrass heights
Soybean yield
kg ai ha−1
28 DAT (%)
At harvest (%)
kg ha−1
0.5
48a
77a
2490b
0.6
37b
62b
2620a
aData pooled over POST1 glufosinate application rate of 0.5, 0.6, or 0.7 kg ai ha−1 and POST2 and POST3 sequential glufosinate application timing.
bMeans followed by the same letter within each column are not significantly different based on Tukey’s HSD at P
≤
0.05.


## 3.4. Soybean Yield

Soybean yields were 2400, 2600, and 2670 kg ha−1 following POST1 at 0.5, 0.6, and 0.7 kg ha−1, respectively, but only yields following the 0.5 and 0.7 kg ha−1 rates differed (Table 2). Similarly, increasing the sequential glufosinate application rate from 0.5 to 0.6 kg ha−1 increased soybean yield 130 kg ha−1 (Table 4). No difference was observed between sequential glufosinate application timings (P
=
0.5869) with soybean yielding 2440 and 2550 kg ha−1 following POST2 and POST3, respectively (data not shown). Conversely, Johnson et al. [41] observed no differences in glufosinate-resistant soybean yield following herbicide programs for johnsongrass control (2,690 to 3,160 kg ha−1); however, differences might have been observed if their studies had not been sprayed with clethodim 10 WAE.


## 4. Discussion

The purpose of these studies was to evaluate glufosinate application rates and timings for control of GR rhizomatous johnsongrass. These data indicate that applying glufosinate POST1 at 0.7 kg ha−1 followed by a sequential application of 0.6 kg ha−1 POST3 provided the greatest control and reduction in GR rhizomatous johnsongrass height throughout the season. Additionally, yields were maximized following 0.7 kg ha−1 of glufosinate POST1 followed by the 0.6 kg ha−1, but no differences were observed between POST2 and POST3. Although data show that glufosinate will control GR rhizomatous johnsongrass, utilization of only one herbicidal site of action (glufosinate in this case) for weed management is not a proper implementation of a herbicide resistance management program. Therefore, we suggest the use of glufosinate as part of a herbicide resistance management program that contains ACCase-inhibiting herbicides, such as clethodim or quizalofop p-ethyl, and acetolactate synthase- (ALS-) inhibiting herbicides, such as imazethapyr or chlorimuron, as POST applications in glufosinate-resistant soybean to manage GR rhizomatous johnsongrass and, potentially, other problematic weeds.



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*Source: 8040235-2016-02-28.xml*