# Reprogramming with Small Molecules instead of Exogenous Transcription Factors

Authors: Tongxiang Lin; Shouhai Wu
Journal: Stem Cells International
 (2015)

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## Abstract

Induced pluripotent stem cells (iPSCs) could be employed in the creation of patient-specific stem cells, which could subsequently be used in various basic and clinical applications. However, current iPSC methodologies present significant hidden risks with respect to genetic mutations and abnormal expression which are a barrier in realizing the full potential of iPSCs. A chemical approach is thought to be a promising strategy for safety and efficiency of iPSC generation. Many small molecules have been identified that can be used in place of exogenous transcription factors and significantly improve iPSC reprogramming efficiency and quality. Recent studies have shown that the use of small molecules results in the generation of chemically induced pluripotent stem cells from mouse embryonic fibroblast cells. These studies might lead to new areas of stem cell research and medical applications, not only human iPSC by chemicals alone, but also safe generation of somatic stem cells for cell based clinical trials and other researches. In this paper, we have reviewed the recent advances in small molecule approaches for the generation of iPSCs.


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## Body

## 1. Introduction

Human induced pluripotent stem cells (iPSCs) are similar to human embryonic stem cells in that they have the potential to differentiate into cells of all three germ layers [1–3]. Elderly patients with injuries, degenerative diseases, or cancers would benefit from stem cell-based regenerative medical techniques. The iPSC applications promise in cell transplantation and stimulate the regenerative medicine of endogenous cells to rebuild tissues,in vitro drug screening, and disease modeling.Initially, adult cells were induced into iPSCs through exogenous overexpression of the transcription factors Oct4 (also known as Pou5f1), Sox2, cMyc, and Klf4. However, efficiency of this technique is at very low level, with around 0.1% of mouse fibroblasts [4] and 0.01% of human fibroblasts cell [2, 5]. The low efficiency and slow dynamics of this method posed serious potential problems for the generation of iPSCs. Besides low iPSC generation efficiency, there are some safety concerns regarding the overexpression of the four aforementioned transcription factors involving genetic mutations, gene insertions, epigenetic changes, incomplete reprogramming, and immunogenicity [6–12].To improve the efficiency and quality of iPSC induction, much effort has been applied in the development of new iPSC generation methods through the use of integrating and nonintegrating recombinant viruses [13–18], DNA expression vectors [19], episomal vectors [20, 21], minicircle vectors [22], and liposomal magnetofection [23]. Non-DNA methods involving proteins [24, 25], mRNA molecules [26], and various chemical agents [27] have also been trialed, and a chemical method that produces chemically induced pluripotent stem cells (CiPSCs) appears to be the most promising methods [27].Although the human iPSC by using chemicals only has not been developed yet, human stem cells studied with small molecules are revealing further details about epigenetic remodeling. Thus, hopefully these researches might relieve concerns about the specificity, efficiency, kinetics, and safety of generating human iPSCs and bring human iPSC closer to effective clinical use [28–30]. We here discuss the small molecules in iPSC generation including three types of compounds: small molecules that may improve reprogramming efficiency; compounds that replace one or more reprogramming factors; compound combination alone that is sufficient to induce mouse iPSC. We also provide perspective views of the possibility of the iPSC generation from human somatic cells and its future applications.


## 2. Compounds That May Improve the Reprogramming Efficiency and Quality

For the first time, Huangfu et al. studied the compound application in iPSC generation; they investigated the effects of the histone deacetylase (HDAC) inhibitor valproic acid (VPA) and found that reprogramming efficiency was increased 100-fold over that of the transcription factor method [31]. Soon, Ding group used BIX-01294, which inhibits histone methyltransferase (HMT) by activating calcium channels in the plasma membrane, to improve reprogramming efficiency [32, 33]. To date, the small molecules that have been used to generate iPSCs can be categorized as epigenetic modifiers, wingless and integration site growth factor (WNT) signal modulators, moderators of cell senescence, or modulators of metabolism [3, 34, 35] and the functions of the small molecules in iPSC generation are summarized in Figure 1. Through these mechanisms, the small molecules could improve iPSC generation efficiency and/or could replace some of Yamanaka factors. The small molecules that might enhance iPSC generation efficiency were collected in Table 1.Table 1
Small molecules that enhance iPSC generation efficiency and quality.
Target or signaling pathway
Name
Concentration
Host cells
Efficiency and necessity
Reference
HDAC inhibitor
VPA
0.5–2 mM
Mouse, human
>100-fold
Huangfu et al. (2008) [31]
HDAC inhibitor
SAHA
5μM
Mouse
10-fold
Huangfu et al. (2008) [31]
HDAC inhibitor
TSA
20 nM
Mouse
10-fold
Huangfu et al. (2008) [31]
HDAC inhibitor
Sodium butyrate
0.5–1 mM
Mouse, human
100-fold
Mali et al. (2010) [66]
DMNT inhibitor
5-aza-CR, AZA
0.5 mM
Mouse
3-fold
Mikkelsen et al. (2008) [29]
DMNT inhibitor, histone deacetylase inhibitor
RSC133
10μM
Human
3-fold
Lee et al. (2012) [10, 67]
Retinoic acid receptor agonist
AM580
100 nM
Mouse
~200-fold
Wang et al. (2011) [68]
H3K4 demethylation inhibitor (epigenetic modulator)
Tranylcypromine (Parnate)
5–10μM
Mouse
3-fold
Li et al. (2009)  [35]
Epigenetic modulators
DZNep
0.05–0.1μM
Mouse
65-fold
Hou et al. (2013) [27]
Retinoic acid receptor ligand
TTNPB
1μM
Mouse
15-fold
Hou et al. (2013) [27]
ALK4, ALK5, and ALK7 inhibitor
SB431542
10 uM
Human
Thiazovivin and PD0325901, ~200 fold
Lin et al. (2009) [62]
Selective MEK/ERK inhibitor
PD0325901
1 uM
Human
Thiazovivin and SB431542, ~200-fold
Lin et al. (2009) [62]
Rho-associated protein kinase inhibitor
Thiazovivin
1 uM
Human
PD0325901 and SB431542, ~200-fold
Lin et al. (2009) [62]
Rho-associated protein kinase inhibitor
Y27632
10 uM
Human
Improve generation and maintaining
Claassen et al. (2009) [69]
AKt-mediated inhibitor of GSK3-β
Compound B6
1μM
Mouse
3-fold
Li et al. (2009) [35]
GSK-3β inhibitor, LSD1 inhibitor
LiCl
5–10 mM
Mouse and human
>10-fold
Wang et al. (2011) [68]
TGF-β inhibitor
A83-01
0.5μM
Mouse, human
7-fold
Zhu et al. (2010) [59]
Prolyl-4-hydroxylase inhibitor
N-Oxalylglycine
1μM
Human
Zhu et al. (2010) [59]
ALK4 inhibitor
Compound B4 (TGFb-RI)
1μM
Mouse
4-fold
Li and Rana (2012) [70]
mTOR inhibitor
Rapamycin
0.3 nM
Mouse
4.8-fold
Chen et al. (2011) [71]
IP3K inhibitor
Compound B8
1-2μM
Mouse
3-fold
Li et al. (2009) [35]
P38 kinase inhibitor
Compound B10
1-2μM
Mouse
3-fold
Li and Rana (2012) [70]
cAMP agonist
Prostaglandin E2
5μM
Mouse
Efficient in mixture
Hou et al. (2013) [27]
cAMP agonist
Rolipram
10μM
Mouse
Efficient in mixture
Hou et al. (2013) [27]
cAMP-dependent protein kinase activator
8-Br-cAMP
0.1–0.5 mM
Human
6.5-fold
Wang et al. (2011) [68]
PDK1 activator
5-(4-Chloro-phenyl)-3-phenyl-pent-2-enoic acid (PS48)
5μM
Human
15-fold
Zhu et al. (2010)  [59]
HIF PHD1 and PHD2 inhibitor
N-Oxalylglycine
1 uM
Human
3.5-fold
Zhu et al. (2010) [59]
Phosphofructokinase 1 activator
Fructose 2,6-bisphosphate
10 mM
Human
2-fold
Zhu et al. (2010) [59]
Hypoxia-inducible factor pathway activator
Quercetin
1μm
Human
3-fold
Zhu et al. (2010) [59]
Oxidative phosphorylation uncoupler
DNP
1μM
Human
2-fold
Zhu et al. (2010) [59]
Note: small molecules can improve reprogramming efficiency by epigenetic modifications or signaling pathway regulation. Many of these small molecules or compound combinations can also replace c-Myc or other transcription factors. DNP, 2,4-dinitrophenol; DZNep, 3-deazaneplanocin; FSK, forskolin; HDAC, histone deacetylase; IP3K, inositol triphosphate 3-kinase; PDK1, phosphoinositide-dependent kinase 1; SAHA, suberoylanilide hydroxamic acid; TF, transcription factor; TSA, trichostatin A; VPA, valproic acid; 2-Me-5HT, 2-methyl-5-hydroxytryptamine; 5-aza-CR, AZA, 5-azacytidine; 8-Br-cAMP, 8-Bromoadenosine cyclic monophosphate.Figure 1
Differentiation and reprogramming are influenced by various mechanisms. Small molecules used to generate iPSCs can be categorized as epigenetic modifiers, WNT signal modulators, cell senescence moderators, modulators of metabolism, and regulators of MET and cell death/senescence pathways. They can influence both differentiation and reprogramming (dedifferentiation).


### 2.1. Epigenetic Modification

During programming, cells undergo changes at a global transcriptional level and also experience epigenetic changes in their DNA, along with histone modifications [3, 28, 29, 34, 36, 37]. Small molecules, such as Bix-01294 (Bix), target enzymes responsible for histone methylation and demethylation and increase expression levels of OCT4 and KLF4 during somatic cell reprogramming [32]. Parnate, a lysine-specific demethylase inhibitor (LSD1), mediates H3K4 demethylation, enabling Oct4 and KLF4 induction of human keratinocytes into iPSCs when combined with CHIR99021, a glycogen synthase kinase 3 (GSK-3) inhibitor [31]. DNA methyltransferase inhibitor 5-azacytidine (5-Aza) or three other histone deacetylase inhibitors (suberoylanilide hydroxamic acid, trichostatin A, and valproic acid) can also improve reprogramming efficiency following the transduction of MEFs with four transcription factors [31, 35, 38, 39].To improve incomplete epigenetic reprogramming, the somatic memory can be erased and new epigenetics are established in iPSCsvia treatment with trichostatin A [38, 39]. Epigenetic modification using small molecules significantly increases iPSC reprogramming efficiency and reduces epigenetic memory retention [3, 34, 39].


### 2.2. WNT Signal Modulators

The WNT pathway plays an important role in self-renewal of embryonic stem cells (ESCs) [40]. The CHIR99021 molecule, an inhibitor of GSK-3β, activates WNT signaling, improving the efficiency of programming and eliminating the use of c-Myc [41].When combined with other compounds, they serve as replacements for certain key transcription factors, or they can induce differentiation into specific cell types. It has been hypothesized that WNT pathway regulators could play a significant role in cellular reprogramming. As an example, the GSK-3 beta inhibitor CHIR99021 can improve reprogramming efficacy by replacing c-Myc. Another GSK3 inhibitor, kenpaullone, can be used as a replacement for KLF4; however, the three transcription factors Oct4, Sox2, and c-Myc must still be used for successful reprogramming [42]. Many similar small molecules have been found to be effective in iPSC generation. The screening of other WNT signaling modulators has been discussed in detail by other researchers [34, 39, 40, 43].


### 2.3. Moderators of Cell Senescence

Cell senescence in reprogramming is usually thought to contribute to slow dynamics and low efficiency. Stress response, cell senescence, and early programming characteristics of iPSCs involve expression of p21cip1 and p16INK4a/p19arf, which are upregulated by p53. Knocking out p53 improves iPSC generation efficiency and kinetics [44–51]. However, the p53 protein is a key tumor suppressor and improving iPSC generation by blocking p53 likely increases the risk of tumor formation [44–50, 52], as shown in murine ESCs [51].A common small molecule, natural antioxidant, vitamin C, has been found to promote the formation of iPSCs from mouse and human somatic cells by indirectly lowering the expression of p21 and p53 [53]. Some small molecules have been found to be regulators of the pathways involved in cell senescence, with little risk of tumor formation [54]. They downregulate the expression of various genes and result in improved efficiency and dynamics during iPSC generation [55–57].


### 2.4. Modulators of Metabolism

Growth of differentiated adult somatic cells usually involves mitochondrial oxidative phosphorylation, while pluripotent stem cells mainly employ glycolysis metabolism [56–58]. This metabolic programming ability is driven by hypoxia-inducible factor 1 alpha, with c-Myc promoting glycolysis [58]. Consistently, PS48, an activator of 3′-phosphoinositide-dependent protein kinase 1, in combination with other small molecules (a-83-01 and PD0325901) and sodium butyrate (a histone deacetylase inhibitor) has been used to reprogram adult keratinocytes, umbilical vein endothelial cells, and amniotic fluid-derived cells. It has been shown that PS48 activates the phosphoinositide 3-kinase/Akt pathway, upregulating gene expression which promotes mitochondrial oxidative phosphorylation and glycolysis metabolism [59].


### 2.5. MET Mediated by Transforming Growth Factor- (TGF-)β Pathway Signaling Inhibitors Enhances Reprogramming

A mesenchymal-epithelial transition (MET) is a reversible biological process involving the transition from motile multipolar or spindle-shaped mesenchymal cells to planar arrays of polarized epithelial cells. Reprogramming of fibroblast cells or iPSCs inevitably involves MET, with cells at various stages of reprogramming undergoing morphological changes towards an epithelial-like cell type [60, 61].Several researchers have shown that inhibition of the TGF-β signaling pathway enhances reprogramming through derepressing the mesenchymal phenotype and inducing MET. By combining two small molecules, SB431542 (an inhibitor of the TGF-β receptor) and PD0325901 (a MEK inhibitor), we demonstrated a 100-fold improvement of efficiency of human iPSC generation [62].Further features of MET were revealed in three other studies where small molecules were used to inhibit TGF-β signaling or E-cadherin upregulation. Among the many TGF-β inhibitors, E-616452 (also known as Repsox) was recently found to be a functional substitute for SOX2 in mouse fibroblast reprogramming with OKM; it also indirectly enhanced NANOG expression during the late stages of reprogramming [63]. The TGF-β receptor inhibitor, a-83-01, combined with the protein arginine methyltransferase inhibitor, AMI-5, along with OCT4, has also been found to promote reprogramming [64]. Small molecule modulators of signal pathways, alone or in combination, and sometimes with epigenetic modifications induced by exogenous transcription factors, affect reprogramming efficiency through the influence of an integrated cellular network.


## 2.1. Epigenetic Modification

During programming, cells undergo changes at a global transcriptional level and also experience epigenetic changes in their DNA, along with histone modifications [3, 28, 29, 34, 36, 37]. Small molecules, such as Bix-01294 (Bix), target enzymes responsible for histone methylation and demethylation and increase expression levels of OCT4 and KLF4 during somatic cell reprogramming [32]. Parnate, a lysine-specific demethylase inhibitor (LSD1), mediates H3K4 demethylation, enabling Oct4 and KLF4 induction of human keratinocytes into iPSCs when combined with CHIR99021, a glycogen synthase kinase 3 (GSK-3) inhibitor [31]. DNA methyltransferase inhibitor 5-azacytidine (5-Aza) or three other histone deacetylase inhibitors (suberoylanilide hydroxamic acid, trichostatin A, and valproic acid) can also improve reprogramming efficiency following the transduction of MEFs with four transcription factors [31, 35, 38, 39].To improve incomplete epigenetic reprogramming, the somatic memory can be erased and new epigenetics are established in iPSCsvia treatment with trichostatin A [38, 39]. Epigenetic modification using small molecules significantly increases iPSC reprogramming efficiency and reduces epigenetic memory retention [3, 34, 39].


## 2.2. WNT Signal Modulators

The WNT pathway plays an important role in self-renewal of embryonic stem cells (ESCs) [40]. The CHIR99021 molecule, an inhibitor of GSK-3β, activates WNT signaling, improving the efficiency of programming and eliminating the use of c-Myc [41].When combined with other compounds, they serve as replacements for certain key transcription factors, or they can induce differentiation into specific cell types. It has been hypothesized that WNT pathway regulators could play a significant role in cellular reprogramming. As an example, the GSK-3 beta inhibitor CHIR99021 can improve reprogramming efficacy by replacing c-Myc. Another GSK3 inhibitor, kenpaullone, can be used as a replacement for KLF4; however, the three transcription factors Oct4, Sox2, and c-Myc must still be used for successful reprogramming [42]. Many similar small molecules have been found to be effective in iPSC generation. The screening of other WNT signaling modulators has been discussed in detail by other researchers [34, 39, 40, 43].


## 2.3. Moderators of Cell Senescence

Cell senescence in reprogramming is usually thought to contribute to slow dynamics and low efficiency. Stress response, cell senescence, and early programming characteristics of iPSCs involve expression of p21cip1 and p16INK4a/p19arf, which are upregulated by p53. Knocking out p53 improves iPSC generation efficiency and kinetics [44–51]. However, the p53 protein is a key tumor suppressor and improving iPSC generation by blocking p53 likely increases the risk of tumor formation [44–50, 52], as shown in murine ESCs [51].A common small molecule, natural antioxidant, vitamin C, has been found to promote the formation of iPSCs from mouse and human somatic cells by indirectly lowering the expression of p21 and p53 [53]. Some small molecules have been found to be regulators of the pathways involved in cell senescence, with little risk of tumor formation [54]. They downregulate the expression of various genes and result in improved efficiency and dynamics during iPSC generation [55–57].


## 2.4. Modulators of Metabolism

Growth of differentiated adult somatic cells usually involves mitochondrial oxidative phosphorylation, while pluripotent stem cells mainly employ glycolysis metabolism [56–58]. This metabolic programming ability is driven by hypoxia-inducible factor 1 alpha, with c-Myc promoting glycolysis [58]. Consistently, PS48, an activator of 3′-phosphoinositide-dependent protein kinase 1, in combination with other small molecules (a-83-01 and PD0325901) and sodium butyrate (a histone deacetylase inhibitor) has been used to reprogram adult keratinocytes, umbilical vein endothelial cells, and amniotic fluid-derived cells. It has been shown that PS48 activates the phosphoinositide 3-kinase/Akt pathway, upregulating gene expression which promotes mitochondrial oxidative phosphorylation and glycolysis metabolism [59].


## 2.5. MET Mediated by Transforming Growth Factor- (TGF-)β Pathway Signaling Inhibitors Enhances Reprogramming

A mesenchymal-epithelial transition (MET) is a reversible biological process involving the transition from motile multipolar or spindle-shaped mesenchymal cells to planar arrays of polarized epithelial cells. Reprogramming of fibroblast cells or iPSCs inevitably involves MET, with cells at various stages of reprogramming undergoing morphological changes towards an epithelial-like cell type [60, 61].Several researchers have shown that inhibition of the TGF-β signaling pathway enhances reprogramming through derepressing the mesenchymal phenotype and inducing MET. By combining two small molecules, SB431542 (an inhibitor of the TGF-β receptor) and PD0325901 (a MEK inhibitor), we demonstrated a 100-fold improvement of efficiency of human iPSC generation [62].Further features of MET were revealed in three other studies where small molecules were used to inhibit TGF-β signaling or E-cadherin upregulation. Among the many TGF-β inhibitors, E-616452 (also known as Repsox) was recently found to be a functional substitute for SOX2 in mouse fibroblast reprogramming with OKM; it also indirectly enhanced NANOG expression during the late stages of reprogramming [63]. The TGF-β receptor inhibitor, a-83-01, combined with the protein arginine methyltransferase inhibitor, AMI-5, along with OCT4, has also been found to promote reprogramming [64]. Small molecule modulators of signal pathways, alone or in combination, and sometimes with epigenetic modifications induced by exogenous transcription factors, affect reprogramming efficiency through the influence of an integrated cellular network.


## 3. Compounds That Can Replace Reprogramming Factors

To date, many studies on small molecules that could be used to replace reprogramming transcription factors have been published. Most of the small molecules might improve reprogramming efficiency and also could replace some functions of one or more Yamanaka factors, Oct4, Sox2, Klf4, c-Myc, or their combinations; we summarize the representative studies (Table2).Table 2
Small molecule compounds that might replace Yamanaka factors.
Replacementfor TF
Nameconcentration
Concentration
Host cell species
Function or target
Reference
Oct4, Nanog
SAHA-PIP delta
100 nM
Mouse
Histone deacetylase inhibitor
Pandian et al. (2014) [65]
Sox2 (with BIX) or Oct4
RG108
0.04–500μM
Mouse
DMNT inhibitor
Shi et al. (2008) [32, 33, 72]
Oct4
BIX
0.5–2μM
Mouse
G9a HMTase inhibitor
Shi et al. (2008) [32, 33, 72]
Sox2
CHIR
3–10μM
Mouse, human
GSK-3β inhibitor that activates Wnt signalling pathway
Li et al. (2011) [73]
Klf4
Kenpaullone
5μM
Mouse
GSK-3/CDKs inhibitor
Lyssiotis et al. (2009) [42]
Sox2
616452 (E-616452, Repsox)
1μM
Mouse, human
TGF-β inhibitor (ALK inhibitor II)
Ichida et al. (2009) [63]
Sox2
LY-364947
1μM
Mouse
TGF-β inhibitor
Staerk et al. (2011) [74]
Sox2, Klf4 (with A-83-01)
AMI-5
5μM
Mouse
Protein arginine methyltransferase inhibitor
Yuan et al. (2011) [64]
Sox2
Dasatinib
0.5μM
Mouse
Src family tyrosine kinase inhibitor
Staerk et al. (2011) [74]
Sox2
iPYrazine (iPY)
10μM
Mouse
Src family tyrosine kinase inhibitor
Staerk et al. (2011) [74]
Sox2
PP1
10μM
Mouse
Src family tyrosine kinase inhibitor
Staerk et al. (2011) [74]
Oct 4 with FSK and 2-Me-5HT
D4476
5μM
Mouse
CK1 inhibitor
Hou et al. (2013) [27]
Sox2
BayK
2μM
Mouse
An L-channel calcium agonist
Shi et al. (2008) [32, 33, 72]
Oct4, when used with 2-Me-5HT, and D4476
FSK
10–50μM
Mouse
cAMP agonist
Hou et al. (2013) [27]
Oct4 with FSK and D4476
2-Me-5HT
5μM
Mouse
5-HT3 agonist
Hou et al. (2013) [27]
Sox2, Klf4, and C-Myc
Oxysterol
0.5–1μM
Mouse
Sonic hedgehog signaling
Moon et al. (2011) [75]
Sox2, Klf4, and C-Myc
Purmorphamine
0.5–1μM
Mouse
Sonic hedgehog signaling
Moon et al. (2011) [75]
Sox2, Klf4, and C-Myc
Shh
500 ng/mL
Mouse
Sonic hedgehog signaling
Moon et al. (2011) [75]
Note: small molecules can substitute for certain TFs and/or improve reprogramming efficiency by epigenetic modifications or signaling pathway regulation. BayK, Bay K8644; BIX, BIX-01294; CHIR, CHIR99021; CK1, casein kinase 1; DNMT, DNA methyltransferase; DNP, 2,4-dinitrophenol; DZNep, 3-deazaneplanocin; FSK, forskolin; HDAC, histone deacetylase; G9a HMTase, G9a histone ethyltransferase; IP3K, inositol triphosphate 3-kinase; PDK1, phosphoinositide dependent kinase 1; SAHA, suberoylanilide hydroxamic acid; TF, transcription factor; TSA, trichostatin A; VPA, valproic acid; 2-Me-5HT, 2-methyl-5-hydroxytryptamine; 5-aza-CR, AZA, 5-azacytidine; 8-Br-cAMP, 8-bromoadenosine cyclic monophosphate.


### 3.1. Compounds That Can Replace Oct4 in Reprogramming

Oct4 is the master regulatory pluripotency gene and may serve as a pluripotency determinant in reprogramming. Several compounds have been claimed that might replace Oct4 expression (Table2). BIX01294, a G9a HMTase inhibitor, was first reported to induce miPSCs in place of Oct4 [32]. RG108, a DNMT inhibitor, can replace Oct4 during mouse skeletal muscle cell reprogramming into miPSCs where skeletal muscle cells endogenously express Sox2, Klf4, and c-Myc [33].Cellular reprogramming involves profound alterations in genome-wide gene expression that is precisely controlled by a hypothetical epigenetic code that can be created artificially by epigenetic modification in a sequence dependent manner with small molecules. A recent report claimed that a specific DNA binding hairpin pyrrole-imidazole polyamides (PIPs) could be conjugated with chromatin modifying histone deacetylase inhibitors like SAHA to epigenetically activate certain pluripotent genes in mouse fibroblasts and identified a novel compound termed SAHA-PIP delta [65]. It could dramatically induce the endogenous expression of Oct-3/4 and Nanog and other pluripotency associated genes; thereby the cells rapidly overcame the rate-limiting step of epithelial transition in cellular reprogramming by switching “ON” the complex transcriptional gene network [65].However, Oct4 could also be replaced by other compounds, such as FSK under binodal “Seesaw” model (Hou et al. and Shu et al.). This mechanism is mentioned in detail in the section of the CiPSC. Many of the small molecules used to replace Oct4 might fall into this category.


### 3.2. Small Molecules That Might Replace Sox2

The compounds 616452 (E-616452, Repsox) and SB431542 are transforming growth factor- (TGF-) beta inhibitors that could replace Sox2 during mouse and human iPSC generation [63]. However, 616452 does not actually act by inducing Sox2 expression in the target cells; rather, it enables reprogramming through the induction of Nanog transcription as reported. Another TGF-b inhibitor, LY-364947, can replace Sox2 in miPSC generation [74].BayK8644 (BayK), an L-channel calcium agonist, was also reported to be able to replace Sox2 in combination with BIX01294 during MEF reprogramming into miPSCs [32, 33, 72]. Shh, purmorphamine, and oxysterol, the activator of Sonic hedgehog signaling, have been reported to upregulate Bmi1, Sox2, and N-Myc expression in mouse fibroblasts [75].Staerk et al. applied a cell-based, high-throughput chemical screening method to identify small molecules that can replace Sox2 during mouse somatic cell reprogramming [74]. From their Nanog reporter-based screening, they discovered that the Pan-Src family kinase inhibitors  iPYrazine, dasatinib, and PP1 could replace Sox2 in MEF reprogramming into miPSCs [74].


### 3.3. Small Molecules That Might Replace Klf4 and c-Myc

While WNT signaling pathway regulators can improve iPSC generation efficiency, they also could replace the function of the c-Myc. Furthermore, several small molecules can increase the iPSC generation efficiency by replacing Klf4 and c-Myc during somatic cell reprogramming into iPSCs. Huangfu et al. reprogrammed MEFs into miPSCs using VPA and transducing three factors without introducing the oncogene c-My [31]. Kenpaullone has been reported as a substitute for Klf4 in mouse cells, although the underlying mechanism is unknown [42].


### 3.4. Small Molecules That Might Replace Sox2, Klf4, and c-Myc

Li et al. succeeded in generating miPSCs by transduction of Oct4 alone with the addition of VPA, CHIR99021, 616452, and tranylcypromine (VC6T) in the culture medium [73]. It is thought that the small-molecule combination including VC6T facilitated miPSC generation by lowering several major barriers to the reprogramming process. Really, the first iPSC generation protocol by using complete chemical alone is based on this protocol [27]. Similarly, many other small molecules combinations were also reported [29, 64, 66–68, 70–72, 75–79].


### 3.5. Combination of 2i Provides Mouse ESC Ground Status for Mature Mouse iPSC

As reported before, the combination of chir99021 (GSK-3 beta inhibitor) and PD0325901 (mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor), designated 2i, is required to maintain the pluripotent ground state in mouse ES cells [80]. Hou et al. cultured the partially reprogrammed cells in 2i medium with leukemia inhibitory factor which induced the stable upregulation of Oct4 and Nanog, transgene silencing, and competence for somatic and germline chimerism; thereby the cells were completely reprogrammed into mouse CiPSCs [27].


## 3.1. Compounds That Can Replace Oct4 in Reprogramming

Oct4 is the master regulatory pluripotency gene and may serve as a pluripotency determinant in reprogramming. Several compounds have been claimed that might replace Oct4 expression (Table2). BIX01294, a G9a HMTase inhibitor, was first reported to induce miPSCs in place of Oct4 [32]. RG108, a DNMT inhibitor, can replace Oct4 during mouse skeletal muscle cell reprogramming into miPSCs where skeletal muscle cells endogenously express Sox2, Klf4, and c-Myc [33].Cellular reprogramming involves profound alterations in genome-wide gene expression that is precisely controlled by a hypothetical epigenetic code that can be created artificially by epigenetic modification in a sequence dependent manner with small molecules. A recent report claimed that a specific DNA binding hairpin pyrrole-imidazole polyamides (PIPs) could be conjugated with chromatin modifying histone deacetylase inhibitors like SAHA to epigenetically activate certain pluripotent genes in mouse fibroblasts and identified a novel compound termed SAHA-PIP delta [65]. It could dramatically induce the endogenous expression of Oct-3/4 and Nanog and other pluripotency associated genes; thereby the cells rapidly overcame the rate-limiting step of epithelial transition in cellular reprogramming by switching “ON” the complex transcriptional gene network [65].However, Oct4 could also be replaced by other compounds, such as FSK under binodal “Seesaw” model (Hou et al. and Shu et al.). This mechanism is mentioned in detail in the section of the CiPSC. Many of the small molecules used to replace Oct4 might fall into this category.


## 3.2. Small Molecules That Might Replace Sox2

The compounds 616452 (E-616452, Repsox) and SB431542 are transforming growth factor- (TGF-) beta inhibitors that could replace Sox2 during mouse and human iPSC generation [63]. However, 616452 does not actually act by inducing Sox2 expression in the target cells; rather, it enables reprogramming through the induction of Nanog transcription as reported. Another TGF-b inhibitor, LY-364947, can replace Sox2 in miPSC generation [74].BayK8644 (BayK), an L-channel calcium agonist, was also reported to be able to replace Sox2 in combination with BIX01294 during MEF reprogramming into miPSCs [32, 33, 72]. Shh, purmorphamine, and oxysterol, the activator of Sonic hedgehog signaling, have been reported to upregulate Bmi1, Sox2, and N-Myc expression in mouse fibroblasts [75].Staerk et al. applied a cell-based, high-throughput chemical screening method to identify small molecules that can replace Sox2 during mouse somatic cell reprogramming [74]. From their Nanog reporter-based screening, they discovered that the Pan-Src family kinase inhibitors  iPYrazine, dasatinib, and PP1 could replace Sox2 in MEF reprogramming into miPSCs [74].


## 3.3. Small Molecules That Might Replace Klf4 and c-Myc

While WNT signaling pathway regulators can improve iPSC generation efficiency, they also could replace the function of the c-Myc. Furthermore, several small molecules can increase the iPSC generation efficiency by replacing Klf4 and c-Myc during somatic cell reprogramming into iPSCs. Huangfu et al. reprogrammed MEFs into miPSCs using VPA and transducing three factors without introducing the oncogene c-My [31]. Kenpaullone has been reported as a substitute for Klf4 in mouse cells, although the underlying mechanism is unknown [42].


## 3.4. Small Molecules That Might Replace Sox2, Klf4, and c-Myc

Li et al. succeeded in generating miPSCs by transduction of Oct4 alone with the addition of VPA, CHIR99021, 616452, and tranylcypromine (VC6T) in the culture medium [73]. It is thought that the small-molecule combination including VC6T facilitated miPSC generation by lowering several major barriers to the reprogramming process. Really, the first iPSC generation protocol by using complete chemical alone is based on this protocol [27]. Similarly, many other small molecules combinations were also reported [29, 64, 66–68, 70–72, 75–79].


## 3.5. Combination of 2i Provides Mouse ESC Ground Status for Mature Mouse iPSC

As reported before, the combination of chir99021 (GSK-3 beta inhibitor) and PD0325901 (mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor), designated 2i, is required to maintain the pluripotent ground state in mouse ES cells [80]. Hou et al. cultured the partially reprogrammed cells in 2i medium with leukemia inhibitory factor which induced the stable upregulation of Oct4 and Nanog, transgene silencing, and competence for somatic and germline chimerism; thereby the cells were completely reprogrammed into mouse CiPSCs [27].


## 4. Compound Cocktails Required to Generate Chemical iPSCs (CiPSCs)

For the first time in history, Hou et al. obtained chemically induced pluripotent stem cells from mouse embryonic fibroblast cells after extensive screening compounds and compound cocktails (Table3).Table 3
Small molecules (VC6TFZ and 2i) that are used in mouse CiPSC.
Target or signaling pathway
Name and concentration
TF to be replaced
Efficiency
Reference
TGF beta pathwayALK5 inhibitor
Repsox (616452)5–10 uM
Sox2, Myc
Essential
Hou et al. (2013) [27] Ichida et al. (2009) [63]
PKA agonist
Forskolin20–50 uM
Oct4 expression (with SKM)Klf2, klf4 expression
Essential
Hou et al. (2013) [27]
WNT pathway regulator, GSK3 beta inhibitor
Chir9902110 uM
Sox2, Myc
Essential
Hou et al. (2013) [27]
Histone methylation modulator, lysine methyltransferase EZH2 inhibitor
DENep50–100 nM
Increase reprogramming
Essential
Hou et al. (2013) [27]
Not specific
TTNPB5 uM
Nuclease receptor signaling modulator
More efficient
Hou et al. (2013) [27]
Not specific
VPA0.5 mM
Histone deacetylase inhibitor
More efficient
Hou et al. (2013) [27]
PD0325901: selective MEK/ERK inhibitorChir99021: WNT pathway regulator, GSK3 beta inhibitor
2i:PD03259011 uMChir9902110 uM
Increase Oct4, Nanog, Sox2 expression
More mature
Hou et al. (2013) [27]
Small molecules can substitute for all TFs through epigenetic modifications and signaling pathway regulations. Hou et al. [27] reported that CiPSC generation from mEF was carried out in 3 steps of 16–20 days in VC6TF treatment and then 12–20 days in VC6TFZ and followed by 2i compounds regulations for 1 week. The abbreviations of the small molecules are shown in Tables 1 and 2.The CiPSC was induced through a very complicated procedure with 3-step compound treatment shown in Figure2. To start, they searched for small molecules that enabled mEF reprogramming in the absence of Oct4 using mEF expressing Oct4 promoter-driven GFP to identify small molecules [27]. Three small molecules, Forskolin (FSK or F), 2-methyl-5-hydroxytryptamine, and D4476, were chemical substitutes for Oct4 after screening 10 000 compounds. FSK was then chosen for subsequent studies. Their early findings showed that the compounds cocktail “VC6T” (VPA, CHIR99021, 616452, and tranylcypromine) could induce mouse iPSC with a single-gene transduction of Oct4. Thus, they combined these two groups of small molecules into a cocktail, VC6TF, resulting in cells expressing E-cadherin [27]. However, Oct4 and NANOG expression were not detectable in these cells, suggesting that reprogramming was incomplete [27].Figure 2
Small molecules based iPSC generation. Small molecules can substitute for all TFs through epigenetic modifications and signaling pathway regulations. Hou et al. reported that CiPSC generation from mEF was carried out in 3 steps of 16–20 days in VC6TF treatment and then 12–20 days in VC6TFZ and followed by 2i compounds regulations for 1 week. The somatic cell, mouse embryonic fibroblast (mEF) cell, undergoes dedifferentiation and gains multipotent stem cell characteristics under the treatments of VC6TF and VC6TFZ steps and finally obtains the full pluripotency in the medium containing 2i compounds (Chir99021 and PD0325901).They found one more compound, 3-deazaneplanocin (DZNep or Z, epigenetic modulator), whose chemical induced stem cell medium contained VC6TFZ [27]. To enhance more pluripotent stem cell gene expression, they furthermore treated the cell in mouse pluripotent ground status conditions with ES medium containing LIF and PD0325901 and CHIR99021 (2i medium).They designed CiPSC protocol including 3 steps as follows: (a) the mEF cells were cultured in mESC medium containing VC6FT for 16–20 days; (b) the cells were cultured in the medium with VC6FTZ for 12–20 days; (c) the cells were cultured in mESC medium containing 2i (PD0325901 and Chir99021) for 1 week. The characteristics of CiPSCs resembled mESCs in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. Therefore, they completed the CiPSC prepared from mEF solely using a combination of seven small-molecule compounds without using transduction or transfection of TFs [27].The group also found that among seven compounds, individual small molecule of C, 6, F, Z was the most essential ingredient to CiPSC; removal of any one of these compounds from the cocktail might result in failure of CiPSC [27]. While these data are very interesting and clearly showed that CiPSC was generated from mEF, the principle of the CiPSC is not clear. To explore the mechanism of the small molecules based iPSC, the research group reported new findings on mechanisms of the pluripotent stem cell status [37].For years, it was generally believed that ESCs are maintained by a shield of pluripotency factors which function in concert with each other to prevent ESCs from differentiating into any lineage, thus preserving ESCs at an undifferentiated state. Noting that some small molecules could replace the Yamanaka factors individually, the combination of these small molecules should be sufficient to replace the Yamanaka Factors. Unfortunately, while some compounds could induce Oct4 expression, the key iPSC transcription factors and the other Yamanaka factors could not be replaced by a single set of small molecules. Therefore, Deng group performed many studies to discover the chemical based iPSC generation mechanism; a binodal “Seesaw” model for cell fate determination then was discovered [37].Shu et al. proposed a new model, termed the “Seesaw” model, in which the pluripotent state is a precarious balancing equilibrium that results from continuous mutual competition between rival lineage specification forces. While the reprogramming factors that induce pluripotency have been identified primarily from embryonic stem cell- (ESC-) enriched pluripotency-associated factors, pluripotency can be induced with lineage specifiers that suppress ESC identity using pluripotency rivals, most of which are not enriched in ESCs. They found that OCT4 and SOX2, the core regulators of pluripotency, can be replaced by lineage specifiers that are involved in mesendodermal (ME) and ectodermal (ECT) specification, respectively [37].OCT4 and its substitutes attenuated the elevated expression of a group of ECT genes, whereas SOX2 and its substitutes curtailed a group of ME genes during reprogramming. Surprisingly, the two counteracting lineage specifiers can synergistically induce pluripotency in the absence of both OCT4 and SOX2 [37]. Therefore, they concluded that the principle of the mouse CiPSC is that the groups of counteracting lineage specifier compounds, VC6FTZ, which belong to 2 counteracting lineage specifiers, were combined to induce the CiPSC [37].After mouse CiPSC, the next logical step is to try using the CiPSC approach to make human stem cells. This binodal model shed light on fundamental questions regarding the establishment of cellular identity during programming in mouse. However, there are a lot of differences between mouse pluripotent stem cells and human ones. For example, leukemia inhibitory factor, LIF, is a necessary factor for mouse pluripotent stem cell, whereas the bFGF is a key essential supplement for human pluripotent stem cell culture. The subtle “Seesaw” balances of the pluripotent factors might need to be adjusted to specific conditions suitable for human CiPSC.


## 5. Conclusions and Perspectives

### 5.1. Is the Combination of Small Molecule Compounds Alone Sufficient for Human iPSC Generation?

To date, small molecules based iPSC have brought dramatically changes to iPSC research. Some of these small molecules increase reprogramming efficiency and quality, while others or combinations of them replace iPSC factors. Mouse CiPSCs were obtained, and thus the complete small molecule-based reprogramming, in a directed and deterministic manner, has fundamentally changed the reprogramming paradigm through a mechanism that involves the activation of endogenous transcription factors by small molecules instead of exogenously expressed transcription factors. Is the combination of small molecule compounds alone sufficient for human iPSC generation?


### 5.2. Extensively Screening for Novel Oct4 Substitutes and Other ESC-Related Transcription Factors

To our knowledge, hiPSC generation with small molecule cocktails has not yet been reported. While most factors used as reprogramming transgenes can be replaced by other means, Oct4 remained essential until the development of human CiPSC technology. Furthermore, the Oct4 expression level in the mouse CiPSCs was reported much lower than those in the mouse ESCs. In human iPSC generation, activation of Oct4 expression and reaching a high enough level similar to that in human ESC are a key challenge. New specific small molecule targeted to activation of reprogramming factors, such as Oct4, might be useful strategy to solve this problem as recent report [65]. Extensively screening for small molecules and compound modifications for novel Oct4 substitutes and other ESC-related transcription factors will cast light on the discovery of molecular mechanisms for reprogramming and transdifferentiation, which in turn facilitate the development of safer and more efficient stem cell resource for clinical applications.


### 5.3. Mechanisms Governing Human CiPSC Generation Might Need to Be Discovered

Deng group found that the two counteracting lineage specifiers can synergistically induce pluripotency in the absence of both OCT4 and SOX2 [37]. While the Seesaw model might also work in human iPSC generation, human pluripotent stem cell ground status conditions might be very different from mouse ones. For example, leukemia inhibitory factor, LIF, is necessary factor for mouse pluripotent stem cell, whereas the bFGF is a key essential supplement for human pluripotent stem cell culture. As a result, similar “seesaw model” of the pluripotent factors needs to be confirmed in human iPSC, and different mechanisms that specifically govern the human CiPSC generation might need to be discovered.In addition, complete reprogramming in mouse CiPSC need mouse ESC ground status compound combination of chir99021 and PD0325901, designated 2i, are required to maintain the pluripotent ground state [80] and confirmed it is necessary for mouse CiPSC. However, 2i is useful but not sufficient in maintaining the ground state for human ESCs, while the compound Chir99021 is useful for iPSC proliferation when it is used with three small molecules during two-step iPSC induction [81].Furthermore, the human CiPSC generation is so complicated that it needs a lot of cellular reorganization, signaling pathways changes, and extracellular matrix maintaining conditions to achieve final reprogrammed pluripotent stem cell status (Figure3). The cells were induced by chemicals through epigenetic modifications that switch on pluripotency transcription factors, such as Oct4, Nang, Sox2, Klf4, and c-Myc, and were kept on through chromatin remodeling. Conversely, genes responsible for differentiation must be turned off by the transcription machinery and kept silent through epigenetic mechanisms. While critical chemicals for pluripotency were VC6TFZ (C6FZ were essential) in mouse CiPSC generation, we might have to regulate more cell signaling pathways including TGF beta, WNT, ERK, ROCK, mitochondria, and other signaling pathways as found in human iPSC generation through viral or nonviral transfection till now. Therefore, waiting to be discovered mechanisms might also be a lot more, including cell signaling pathways as well as nuclear and mitochondria genomic activities (Figure 3).Figure 3
Human CiPSC perspective regulation pathways. The human CiPSC generation is so complicated that needs a lot of cellular reorganization, signaling pathways changes, and extracellular matrix maintaining conditions to achieve final reprogrammed pluripotent stem cell status. The cells were induced by chemicals through epigenetic modifications that switch on pluripotency transcription factors, such as Oct4, Nang, Sox2, Klf4, and c-Myc. The cell signaling pathways including TGF beta, WNT, ERK, ROCK, mitochondria, and other signaling pathways might need to be regulated in human CiPSC generation. However, waiting to be discovered mechanisms might also be some other unknown cell signaling pathways as well as nuclear and mitochondria genomic activities.


### 5.4. Safety Issues, Especially, Tumor Concern, Have to Be Solved before Clinical Applications

Although human iPSCs provide an alternative stem cell resource for regenerative medicine, we still hesitate before using them in clinic applications due to safety concerns, such as tumor risks. The iPSC generation is so complicated that cellular reorganizations, signaling pathways changes, and extracellular matrix maintain conditions to achieve final reprogramming goals. Furthermore, it is extremely demanding to obtain highly pure population of the target cells. The small molecules based approaches might provide solutions for pure target adult stem cell without tumor concerns.


### 5.5. The Small Molecules Research Might Bring Close Future Applications Not Only for iPSC Application but Also for Adult Stem Cell Based Applications

The small molecules in the iPSC generation might also provide useful information for those studies in adult stem cell. Direct reprogramming of one type of adult stem cell to another one was suggested as an attractive alternative for clinical applications. Some studies have developed small molecules that guide human transdifferentiation of tissue-specific progenitor cells or stem cells. Because adult stem cells, such as bone marrow stem cells and hematopoietic stem cells, are ready to be used in clinical applications, the only obstacle is their limited proliferation. The development of small molecules to work in adult stem cell self-renewals or to guide human somatic cells into progenitor cells will open avenues for the clinical application of these types of progenitor cells and stem cells.Advances in the understanding of reprogramming mechanism and continuous development of small molecular tools to enhance reprogramming will not only facilitate the possibility of generating safer and higher quality reprogrammed cells but also provide useful information for adult stem cell based applications.


## 5.1. Is the Combination of Small Molecule Compounds Alone Sufficient for Human iPSC Generation?

To date, small molecules based iPSC have brought dramatically changes to iPSC research. Some of these small molecules increase reprogramming efficiency and quality, while others or combinations of them replace iPSC factors. Mouse CiPSCs were obtained, and thus the complete small molecule-based reprogramming, in a directed and deterministic manner, has fundamentally changed the reprogramming paradigm through a mechanism that involves the activation of endogenous transcription factors by small molecules instead of exogenously expressed transcription factors. Is the combination of small molecule compounds alone sufficient for human iPSC generation?


## 5.2. Extensively Screening for Novel Oct4 Substitutes and Other ESC-Related Transcription Factors

To our knowledge, hiPSC generation with small molecule cocktails has not yet been reported. While most factors used as reprogramming transgenes can be replaced by other means, Oct4 remained essential until the development of human CiPSC technology. Furthermore, the Oct4 expression level in the mouse CiPSCs was reported much lower than those in the mouse ESCs. In human iPSC generation, activation of Oct4 expression and reaching a high enough level similar to that in human ESC are a key challenge. New specific small molecule targeted to activation of reprogramming factors, such as Oct4, might be useful strategy to solve this problem as recent report [65]. Extensively screening for small molecules and compound modifications for novel Oct4 substitutes and other ESC-related transcription factors will cast light on the discovery of molecular mechanisms for reprogramming and transdifferentiation, which in turn facilitate the development of safer and more efficient stem cell resource for clinical applications.


## 5.3. Mechanisms Governing Human CiPSC Generation Might Need to Be Discovered

Deng group found that the two counteracting lineage specifiers can synergistically induce pluripotency in the absence of both OCT4 and SOX2 [37]. While the Seesaw model might also work in human iPSC generation, human pluripotent stem cell ground status conditions might be very different from mouse ones. For example, leukemia inhibitory factor, LIF, is necessary factor for mouse pluripotent stem cell, whereas the bFGF is a key essential supplement for human pluripotent stem cell culture. As a result, similar “seesaw model” of the pluripotent factors needs to be confirmed in human iPSC, and different mechanisms that specifically govern the human CiPSC generation might need to be discovered.In addition, complete reprogramming in mouse CiPSC need mouse ESC ground status compound combination of chir99021 and PD0325901, designated 2i, are required to maintain the pluripotent ground state [80] and confirmed it is necessary for mouse CiPSC. However, 2i is useful but not sufficient in maintaining the ground state for human ESCs, while the compound Chir99021 is useful for iPSC proliferation when it is used with three small molecules during two-step iPSC induction [81].Furthermore, the human CiPSC generation is so complicated that it needs a lot of cellular reorganization, signaling pathways changes, and extracellular matrix maintaining conditions to achieve final reprogrammed pluripotent stem cell status (Figure3). The cells were induced by chemicals through epigenetic modifications that switch on pluripotency transcription factors, such as Oct4, Nang, Sox2, Klf4, and c-Myc, and were kept on through chromatin remodeling. Conversely, genes responsible for differentiation must be turned off by the transcription machinery and kept silent through epigenetic mechanisms. While critical chemicals for pluripotency were VC6TFZ (C6FZ were essential) in mouse CiPSC generation, we might have to regulate more cell signaling pathways including TGF beta, WNT, ERK, ROCK, mitochondria, and other signaling pathways as found in human iPSC generation through viral or nonviral transfection till now. Therefore, waiting to be discovered mechanisms might also be a lot more, including cell signaling pathways as well as nuclear and mitochondria genomic activities (Figure 3).Figure 3
Human CiPSC perspective regulation pathways. The human CiPSC generation is so complicated that needs a lot of cellular reorganization, signaling pathways changes, and extracellular matrix maintaining conditions to achieve final reprogrammed pluripotent stem cell status. The cells were induced by chemicals through epigenetic modifications that switch on pluripotency transcription factors, such as Oct4, Nang, Sox2, Klf4, and c-Myc. The cell signaling pathways including TGF beta, WNT, ERK, ROCK, mitochondria, and other signaling pathways might need to be regulated in human CiPSC generation. However, waiting to be discovered mechanisms might also be some other unknown cell signaling pathways as well as nuclear and mitochondria genomic activities.


## 5.4. Safety Issues, Especially, Tumor Concern, Have to Be Solved before Clinical Applications

Although human iPSCs provide an alternative stem cell resource for regenerative medicine, we still hesitate before using them in clinic applications due to safety concerns, such as tumor risks. The iPSC generation is so complicated that cellular reorganizations, signaling pathways changes, and extracellular matrix maintain conditions to achieve final reprogramming goals. Furthermore, it is extremely demanding to obtain highly pure population of the target cells. The small molecules based approaches might provide solutions for pure target adult stem cell without tumor concerns.


## 5.5. The Small Molecules Research Might Bring Close Future Applications Not Only for iPSC Application but Also for Adult Stem Cell Based Applications

The small molecules in the iPSC generation might also provide useful information for those studies in adult stem cell. Direct reprogramming of one type of adult stem cell to another one was suggested as an attractive alternative for clinical applications. Some studies have developed small molecules that guide human transdifferentiation of tissue-specific progenitor cells or stem cells. Because adult stem cells, such as bone marrow stem cells and hematopoietic stem cells, are ready to be used in clinical applications, the only obstacle is their limited proliferation. The development of small molecules to work in adult stem cell self-renewals or to guide human somatic cells into progenitor cells will open avenues for the clinical application of these types of progenitor cells and stem cells.Advances in the understanding of reprogramming mechanism and continuous development of small molecular tools to enhance reprogramming will not only facilitate the possibility of generating safer and higher quality reprogrammed cells but also provide useful information for adult stem cell based applications.



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*Source: 794632-2015-04-01.xml*