# Leptin Administration Downregulates the Increased Expression Levels of Genes Related to Oxidative Stress and Inflammation in the Skeletal Muscle ofob/ob Mice

Authors: Neira Sáinz; Amaia Rodríguez; Victoria Catalán; Sara Becerril; Beatriz Ramírez; Javier Gómez-Ambrosi; Gema Frühbeck
Journal: Mediators of Inflammation
 (2010)

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## Abstract

Obese leptin-deficientob/ob mice exhibit a
low-grade chronic inflammation together with a low muscle mass.
Our aim was to analyze the changes in muscle expression levels of
genes related to oxidative stress and inflammatory responses in
leptin deficiency and to identify the effect of in
vivo leptin administration. Ob/ob mice
were divided in three groups as follows: control
ob/ob, leptin-treated ob/ob
(1 mg/kg/d) and leptin pair-fed ob/ob
mice. Gastrocnemius weight was lower in control
ob/ob than in wild type mice (P<.01) exhibiting an increase after leptin treatment
compared to control and pair-fed (P<.01) ob/ob animals. Thiobarbituric acid
reactive substances, markers of oxidative stress, were higher in
serum (P<.01) and gastrocnemius (P=.05) of control ob/ob than in wild type
mice and were significantly decreased (P<.01) by leptin treatment. Leptin deficiency altered the
expression of 1,546 genes, while leptin treatment modified the
regulation of 1,127 genes with 86 of them being involved in
oxidative stress, immune defense and inflammatory response. Leptin
administration decreased the high expression of Crybb1,
Hspb3, Hspb7, Mt4, Cat, Rbm9, Serpinc1 and
Serpinb1a observed in control
ob/ob mice, indicating that it improves
inflammation and muscle loss.


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## Body

## 1. Introduction

Obesity is associated with a low-grade proinflammatory state resulting in an increase of circulating cytokines and inflammatory markers [1]. Inflammatory cytokines have been involved in the impairment of insulin signaling, thus providing molecular links between inflammation and insulin resistance [2]. Inflammation reportedly produces metabolic alterations in skeletal muscle with both inflammatory response and insulin resistance being associated with loss of muscle mass by decreased protein synthesis and increased proteolysis [3–5]. Recently, our group has shown that leptin reverses muscle loss of ob/ob mice by inhibiting the activity of the transcriptional factor forkhead box class O3a (FoxO3a) [6].Leptin is an adipocyte-derived peptidic hormone [7] that inhibits food intake and increases thermogenesis by acting through its hypothalamic receptors [8, 9]. Leptin-deficient ob/ob mice are obese, hyperphagic, exhibit type 2 diabetes, decreased body temperature and hypogonadotropic hypogonadism [10]. Leptin is a member of the long-chain helical cytokine family and its receptors, which belong to the class I cytokine receptors, are present in bone marrow and spleen as well as on peripheral monocytes and lymphocytes [1]. Leptin increases in response to acute infection and sepsis and it has been reported to exert a profound influence on the function and proliferation of T lymphocytes and natural killer cells [11], on the phagocytosis of macrophages/monocytes [12], and to have a direct effect on the secretion of anti- and proinflammatory cytokines [13]. In this regard, impaired cellular and humoral immunity have been shown in leptin-deficient ob/ob mice as well as in leptin receptor-deficient db/db mice [14, 15]. These studies reflect the molecular nature of leptin as a cytokine and are consistent with leptin signaling playing a pivotal role in the pathogenesis of obesity-associated inflammation and muscle loss.In the present paper, gastrocnemius muscle samples from wild type andob/ob mice were analyzed for mRNA presence of over 41,000 transcripts by microarray analysis to identify genes involved in inflammation and oxidative stress that are affected by leptin deficiency and leptin administration in ob/ob mice. It was shown that leptin increases the gastrocnemius weight and reduces the high expression levels of genes related to the obesity-associated low-grade inflammation in skeletal muscle of ob/ob mice.


## 2. Material and Methods

### 2.1. Animals and Treatments

Ten-week-old male genetically obeseob/ob mice (C57BL/6J) (n=15) and their lean control littermates wild type (n=5) supplied by Harlan (Barcelona, Spain) were housed in a room with controlled temperature (22±2°C) and a 12:12 light-dark cycle (lights on at 08:00 am). Body weight of ob/ob mice was measured before randomization into control, leptin-treated (1 mg/kg/d) and pair-fed groups (n=5 per group). The control and pair-fed groups received vehicle (PBS), while leptin-treated mice were intraperitoneally administered with leptin (Bachem, Bubendorf, Switzerland) twice daily at 08:00 am and 08:00 pm for 28 days. Control and leptin-treated groups were provided with water and food ad libitum with a standard rodent chow (2014S Teklad, Harlan), while daily food intake of the pair-fed group was matched to the amount consumed by the leptin-treated group the day before in order to discriminate the inhibitory effect of leptin on appetite. Animals were sacrificed on the 28th day of treatment by CO2 inhalation 20 hours after the last PBS or leptin administration (in order to avoid picking up effects reflecting an acute response) and after 8 hours of fasting. Serum samples and gastrocnemius muscles were obtained and stored at -80°C. All experimental procedures conformed to the European Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by the Ethical Committee for Animal Experimentation of the University of Navarra (080/05).


### 2.2. Blood Analysis

Serum glucose was analyzed using a sensitive-automatic glucose sensor (Ascensia Elite, Bayer, Barcelona, Spain). Free fatty acid (FFA) concentrations were measured by a colorimetric determination using the NEFA C kit (WAKO Chemicals, Neuss, Germany). Serum glycerol concentrations were evaluated by enzymatic methods as previously described [6]. Serum triglycerides (TG) concentrations were spectrophotometrically determined using a commercial kit (Infinity, Thermo Electron, Melbourne, Australia). Insulin and leptin were determined using specific mouse ELISA kits (Crystal Chem Inc., Chicago, IL, USA). Intra- and interassay coefficients of variation for measurements of insulin and leptin were 3.5% and 6.3%, respectively, for the former, and 2.8% and 5.8%, for the latter. Adiponectin concentrations were also assessed using a mouse ELISA kit (BioVendor Laboratory Medicine, Inc., Modrice, Czech Republic). Intra- and interassay coefficients of variation for adiponectin were 2.6% and 5.3%, respectively. Insulin resistance was calculated using the homeostasis model assessment score (HOMA; fasting insulin (μU/mL) × fasting glucose (mmol/L)/22.5) [16]. An indirect measure of insulin sensitivity was calculated by using the quantitative insulin sensitivity check index (QUICKI; 1/[log(fasting insulin mU/mL) + log(fasting glucose mg/dL)] [17].Lipid peroxidation was analyzed by the measurement of thiobarbituric acid reactive substances (TBARS) in serum and gastrocnemius as previously described by Conti et al. [18] with some modifications. Since the best-known specific TBARS is malondialdehyde (MDA), we used serum MDA levels, a secondary product of lipid peroxidation, as an indicator of lipid peroxidation and oxidative stress. Gastrocnemius samples (20–30 mg) were homogenized in 20 volumes of phosphate buffer pH 7.4. Serum, muscle homogenates (5 μL) or standard (MDA) were mixed with 120 μL of diethyl thiobarbituric acid (DETBA) 10 mM and vortexed for 5 seconds. The reaction mixture was then incubated at 95°C for 60 minutes. After cooling to room temperature DETBA-MDA adducts were extracted in 360 μL n-butanol vortexing for 1 minute and centrifuged at 1,600 g for 10 minutes at room temperature. Then, the chromophore of the DETBA-MDA adduct was quantified in 200 μL of the upper butanol phase by fluorescence emission at 535 nm with an excitation at 590 nm. MDA equivalents (TBARS) were quantified using a calibration curve prepared using MDA standard working solutions and expressed as serum MDA μM and gastrocnemius MDA μM/mg protein. Protein concentrations were determined using a Bradford protein assay kit (BioRad, Hercules, CA, USA).


### 2.3. Microarray Experiments and Analysis

Total RNA was extracted from 20–30 mg of gastrocnemius muscle samples by homogenization with an ULTRA-TURRAX T 25 basic (IKA Werke GmbH, Staufen, Germany) using TRIzol™ reagent (Invitrogen, Barcelona, Spain). RNA was purified using the RNeasy Mini kit (Qiagen, Barcelona, Spain) and treated with DNase I (RNase-free DNase Set, Qiagen) in order to remove any trace of genomic DNA.Gene expression analyses were conducted using the Agilent Whole Mouse Genome array (G4121B, Agilent Technologies, Santa Clara, CA, USA) containing ~41,000 mouse genes and transcripts. Fluorescence-labeled cDNA probes were prepared from 1 μg of total RNA from each sample (5 animals per group) to be subsequently amino-allyl labeled and amplified using the Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion, Austin, TX, USA). Aliquots (1.2 μg) of amplified aRNA were fluorescently labeled using Cy3/Cy5 (Amersham Biosciences, Buckinghamshire, UK) and then appropriately combined and hybridized to Agilent microarrays. Hybridizations were performed following a reference design, where control samples were pools of RNA from all individual samples. Two hybridizations with fluor reversal (Dye-swap) were performed for each sample. After washing, microarray slides were scanned using a Gene Pix 4100A scanner (Axon Instruments, Union City, CA, USA) and image quantization was performed using the software GenePiX Pro 6.0. Gene expression data for all replicate experiments were analyzed using the GeneSpring GX software version 7.3.1 (Agilent Technologies). Clustering was accomplished with the Gene and Condition Tree algorithms. In addition, Gene Ontology database (http://babelomics.bioinfo.cipf.es) and the KEGG website (http://www.genome.ad.jp/kegg/pathway) were used in conjunction with GeneSpring (http://www.agilent.com/chem/genespring) toidentify pathways and functional groups of genes. All microarray data reported are described in accordance with MIAME guidelines (http://www.mged.org/Workgroups/MIAME/miame.html). More information regarding the microarray experiments can be found at the EMBL-European Bioinformatics Institute (http://www.ebi.ac.uk/aerep/login. ArrayExpress accession number: E-MEXP-1831). To validate the microarray data, a number of representative differentially expressed genes were selected to be individually studied by Real-Time PCR (7300 Real Time PCR System, Applied Biosystems, Foster City, CA, USA) (n=5 per group) as previously described [19]. Primers and probes were designed using the software Primer Express 2.0 (Applied Biosystems) and purchased from Genosys (Sigma, Madrid, Spain) (Table 1).Table 1
Sequences of the primers and Taqman probes used in the Real-Time PCR.
GeneGene SymbolGenBank accesión numberOligonucleotide sequence (5′-3′)Peroxisome proliferator-activated receptor-γ coactivator-1αPgc1aNM_008904Forward: GTCTGAAAGGGCCAAACAGAGAReverse: TCAATTCTGTCCGCGTTGTGProbe: FAM-AGCAGAAAGCAATTGAAGAGCGCCGT-TAMRAForkhead box O1Foxo1NM_019739Forward: GCGGGCTGGAAGAATTCAATReverse: TCCTTCATTCTGCACTCGAATAAAC TProbe: FAM-CGCCACAATCTGTCCCTTCACA-TAMRAMuscle atrophy F boxMAFbxNM_026346Forward: CCATCCTGGATTCCAGAAGATTCReverse: TCAGGGATGTGAGCTGTGACTTTProbe: FAM-CTACGTAGTAAGGCTGTTGGAGCTGAT-TAMRAMuscle RING finger 1MuRF1NM_001039048Forward:  CGCCATGAAGTGATCATGGAReverse: TCCTTGGAAGATGCTTTGCAProbe: FAM-TGTACGGCCTGCAGAGGAACCTGAAA-TAMRA


### 2.4. Statistical Analysis

Data are expressed asmean±standard error of the mean (SEM). Differences between groups were assessed by Kruskal-Wallis followed by Mann Whitney’s U test. As previously outlined, Gene Ontology groupings were used to identify pathways significantly affected by leptin deficiency as opposed to its administration. Furthermore, statistical comparisons for microarray data to identify differentially expressed genes across different groups were performed using one-way ANOVA and Student’s t-tests as appropriate. Spearman’s correlations were used to evaluate the relations among different variables. All statistical analyses were performed by using the SPSS statistical program version 15.0 for Windows (SPSS, Chicago, IL, USA) and statistical significance was defined as P<.05.


## 2.1. Animals and Treatments

Ten-week-old male genetically obeseob/ob mice (C57BL/6J) (n=15) and their lean control littermates wild type (n=5) supplied by Harlan (Barcelona, Spain) were housed in a room with controlled temperature (22±2°C) and a 12:12 light-dark cycle (lights on at 08:00 am). Body weight of ob/ob mice was measured before randomization into control, leptin-treated (1 mg/kg/d) and pair-fed groups (n=5 per group). The control and pair-fed groups received vehicle (PBS), while leptin-treated mice were intraperitoneally administered with leptin (Bachem, Bubendorf, Switzerland) twice daily at 08:00 am and 08:00 pm for 28 days. Control and leptin-treated groups were provided with water and food ad libitum with a standard rodent chow (2014S Teklad, Harlan), while daily food intake of the pair-fed group was matched to the amount consumed by the leptin-treated group the day before in order to discriminate the inhibitory effect of leptin on appetite. Animals were sacrificed on the 28th day of treatment by CO2 inhalation 20 hours after the last PBS or leptin administration (in order to avoid picking up effects reflecting an acute response) and after 8 hours of fasting. Serum samples and gastrocnemius muscles were obtained and stored at -80°C. All experimental procedures conformed to the European Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by the Ethical Committee for Animal Experimentation of the University of Navarra (080/05).


## 2.2. Blood Analysis

Serum glucose was analyzed using a sensitive-automatic glucose sensor (Ascensia Elite, Bayer, Barcelona, Spain). Free fatty acid (FFA) concentrations were measured by a colorimetric determination using the NEFA C kit (WAKO Chemicals, Neuss, Germany). Serum glycerol concentrations were evaluated by enzymatic methods as previously described [6]. Serum triglycerides (TG) concentrations were spectrophotometrically determined using a commercial kit (Infinity, Thermo Electron, Melbourne, Australia). Insulin and leptin were determined using specific mouse ELISA kits (Crystal Chem Inc., Chicago, IL, USA). Intra- and interassay coefficients of variation for measurements of insulin and leptin were 3.5% and 6.3%, respectively, for the former, and 2.8% and 5.8%, for the latter. Adiponectin concentrations were also assessed using a mouse ELISA kit (BioVendor Laboratory Medicine, Inc., Modrice, Czech Republic). Intra- and interassay coefficients of variation for adiponectin were 2.6% and 5.3%, respectively. Insulin resistance was calculated using the homeostasis model assessment score (HOMA; fasting insulin (μU/mL) × fasting glucose (mmol/L)/22.5) [16]. An indirect measure of insulin sensitivity was calculated by using the quantitative insulin sensitivity check index (QUICKI; 1/[log(fasting insulin mU/mL) + log(fasting glucose mg/dL)] [17].Lipid peroxidation was analyzed by the measurement of thiobarbituric acid reactive substances (TBARS) in serum and gastrocnemius as previously described by Conti et al. [18] with some modifications. Since the best-known specific TBARS is malondialdehyde (MDA), we used serum MDA levels, a secondary product of lipid peroxidation, as an indicator of lipid peroxidation and oxidative stress. Gastrocnemius samples (20–30 mg) were homogenized in 20 volumes of phosphate buffer pH 7.4. Serum, muscle homogenates (5 μL) or standard (MDA) were mixed with 120 μL of diethyl thiobarbituric acid (DETBA) 10 mM and vortexed for 5 seconds. The reaction mixture was then incubated at 95°C for 60 minutes. After cooling to room temperature DETBA-MDA adducts were extracted in 360 μL n-butanol vortexing for 1 minute and centrifuged at 1,600 g for 10 minutes at room temperature. Then, the chromophore of the DETBA-MDA adduct was quantified in 200 μL of the upper butanol phase by fluorescence emission at 535 nm with an excitation at 590 nm. MDA equivalents (TBARS) were quantified using a calibration curve prepared using MDA standard working solutions and expressed as serum MDA μM and gastrocnemius MDA μM/mg protein. Protein concentrations were determined using a Bradford protein assay kit (BioRad, Hercules, CA, USA).


## 2.3. Microarray Experiments and Analysis

Total RNA was extracted from 20–30 mg of gastrocnemius muscle samples by homogenization with an ULTRA-TURRAX T 25 basic (IKA Werke GmbH, Staufen, Germany) using TRIzol™ reagent (Invitrogen, Barcelona, Spain). RNA was purified using the RNeasy Mini kit (Qiagen, Barcelona, Spain) and treated with DNase I (RNase-free DNase Set, Qiagen) in order to remove any trace of genomic DNA.Gene expression analyses were conducted using the Agilent Whole Mouse Genome array (G4121B, Agilent Technologies, Santa Clara, CA, USA) containing ~41,000 mouse genes and transcripts. Fluorescence-labeled cDNA probes were prepared from 1 μg of total RNA from each sample (5 animals per group) to be subsequently amino-allyl labeled and amplified using the Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion, Austin, TX, USA). Aliquots (1.2 μg) of amplified aRNA were fluorescently labeled using Cy3/Cy5 (Amersham Biosciences, Buckinghamshire, UK) and then appropriately combined and hybridized to Agilent microarrays. Hybridizations were performed following a reference design, where control samples were pools of RNA from all individual samples. Two hybridizations with fluor reversal (Dye-swap) were performed for each sample. After washing, microarray slides were scanned using a Gene Pix 4100A scanner (Axon Instruments, Union City, CA, USA) and image quantization was performed using the software GenePiX Pro 6.0. Gene expression data for all replicate experiments were analyzed using the GeneSpring GX software version 7.3.1 (Agilent Technologies). Clustering was accomplished with the Gene and Condition Tree algorithms. In addition, Gene Ontology database (http://babelomics.bioinfo.cipf.es) and the KEGG website (http://www.genome.ad.jp/kegg/pathway) were used in conjunction with GeneSpring (http://www.agilent.com/chem/genespring) toidentify pathways and functional groups of genes. All microarray data reported are described in accordance with MIAME guidelines (http://www.mged.org/Workgroups/MIAME/miame.html). More information regarding the microarray experiments can be found at the EMBL-European Bioinformatics Institute (http://www.ebi.ac.uk/aerep/login. ArrayExpress accession number: E-MEXP-1831). To validate the microarray data, a number of representative differentially expressed genes were selected to be individually studied by Real-Time PCR (7300 Real Time PCR System, Applied Biosystems, Foster City, CA, USA) (n=5 per group) as previously described [19]. Primers and probes were designed using the software Primer Express 2.0 (Applied Biosystems) and purchased from Genosys (Sigma, Madrid, Spain) (Table 1).Table 1
Sequences of the primers and Taqman probes used in the Real-Time PCR.
GeneGene SymbolGenBank accesión numberOligonucleotide sequence (5′-3′)Peroxisome proliferator-activated receptor-γ coactivator-1αPgc1aNM_008904Forward: GTCTGAAAGGGCCAAACAGAGAReverse: TCAATTCTGTCCGCGTTGTGProbe: FAM-AGCAGAAAGCAATTGAAGAGCGCCGT-TAMRAForkhead box O1Foxo1NM_019739Forward: GCGGGCTGGAAGAATTCAATReverse: TCCTTCATTCTGCACTCGAATAAAC TProbe: FAM-CGCCACAATCTGTCCCTTCACA-TAMRAMuscle atrophy F boxMAFbxNM_026346Forward: CCATCCTGGATTCCAGAAGATTCReverse: TCAGGGATGTGAGCTGTGACTTTProbe: FAM-CTACGTAGTAAGGCTGTTGGAGCTGAT-TAMRAMuscle RING finger 1MuRF1NM_001039048Forward:  CGCCATGAAGTGATCATGGAReverse: TCCTTGGAAGATGCTTTGCAProbe: FAM-TGTACGGCCTGCAGAGGAACCTGAAA-TAMRA


## 2.4. Statistical Analysis

Data are expressed asmean±standard error of the mean (SEM). Differences between groups were assessed by Kruskal-Wallis followed by Mann Whitney’s U test. As previously outlined, Gene Ontology groupings were used to identify pathways significantly affected by leptin deficiency as opposed to its administration. Furthermore, statistical comparisons for microarray data to identify differentially expressed genes across different groups were performed using one-way ANOVA and Student’s t-tests as appropriate. Spearman’s correlations were used to evaluate the relations among different variables. All statistical analyses were performed by using the SPSS statistical program version 15.0 for Windows (SPSS, Chicago, IL, USA) and statistical significance was defined as P<.05.


## 3. Results

### 3.1. Leptin Treatment Improves the Metabolic Profile of ob/ob Mice

The morphological and biochemical characteristics of wild type andob/ob mice are reported in Table 2. As expected, leptin treatment corrected the obese and diabetic phenotype of ob/ob mice. Body weight was significantly higher (P<.01) in the control ob/ob group as compared to wild type mice. Leptin-treated mice exhibited a decreased body weight (P<.01) as compared to control and pair-fed ob/ob animals. Importantly, leptin treatment normalized body weight of ob/ob mice as compared to wild type (P=.690). In addition, the gastrocnemius of control ob/ob mice exhibited a lower (P<.01) muscle weight than that of wild type mice and it was increased (P<.01) by leptin administration in comparison with that of control and pair-fed ob/ob rodents. As depicted in Table 2, higher fasting glucose (P<.05) and insulin (P<.01) concentrations were observed in the control ob/ob mice compared to wild types. Although no differences in glucose concentrations were observed in pair-fed as compared to leptin-treated ob/ob mice, higher serum insulin concentrations (P<.05) were detected in the pair-fed animals than in the leptin-treated ob/ob group. Furthermore, leptin administration normalized both the glucose and insulin levels in ob/ob mice compared to wild types. These data suggest that leptin increases the insulin sensitivity in peripheral tissues, as evidenced by the lower HOMA and higher QUICKI indices (P<.01) in the leptin-treated in comparison with the control ob/ob animals. Serum glycerol was markedly increased (P<.05) in the control ob/ob mice, while FFA and TG levels remained unchanged as compared to wild type mice. Interestingly, leptin not only decreased circulating concentrations of FFA (P<.05) and glycerol (P<.01) levels as compared to control ob/obmice, but also FFA (P<.01), glycerol (P<.01) and TG (P<.05) concentrations as compared to pair-fed mice. Leptin administration to ob/ob mice reduced serum glycerol concentrations (P=.032) and tended to decrease FFA (P=.095) as compared to wild types. Furthermore, leptin treatment increased the low concentrations of adiponectin of ob/ob mice, but the differences fell out of statistical significance (P=.095).Table 2
Total body and skeletal muscle weights and biochemical characteristics of wild type andob/ob mice.
wild typecontrolob/obpair-fedob/obleptin-treatedob/obBody weight (g)25.6±0.347.8±4.9b35.7±0.724.7±1.2d,fGastrocnemius (mg)142.9±3.490.7±10.0b68.5±1.6104.9±2.6b,fGastrocnemius (mg/g)5.59±0.121.91±0.11b1.92±0.074.28±0.15b,d,fGlucose (mg/dL)149±42430±59a160±24d178±29dFFA (mmol/L)1.62±0.491.61±0.301.65±0.120.78±0.13c,fGlycerol (mmol/L)42.8±6.781.6±19.6a39.6±4.9c12.3±4.7a,d,fTG (mg/dL)122±18169±32151±1086±17eInsulin (ng/mL)0.42±0.098.60±1.51b2.40±0.68c0.47±0.09d,eAdiponectin (μg/mL)30.2±3.028.3±5.439.1±1.840.2±3.0Leptin (ng/mL)1.36±0.42UDUD3.48±1.02HOMA4.3±1.8202.4±33.8b25.8±10.4d5.12±1.1dQUICKI0.333±0.0230.205±0.003b0.263±0.015d0.311±0.016dData aremean±SEM (n=5 per group). Differences between groups were analyzed by Kruskal-Wallis followed by Mann Whitney’s U test. aP<.05 and bP<.01versus wild type. cP<.05 and dP<.01   versus  ob/ob. eP<.05 and fP<.01versus pair-fed ob/ob. FFA: free fatty acids. TG: triglycerides. UD: undetectable. HOMA: homeostasis model assessment. QUICKI: quantitative insulin sensitivity check index.Controlob/ob mice exhibited significantly higher serum TBARS than wild type littermates (P<.01), which were significantly reduced after leptin administration as compared to the control (P<.01) and pair-fed (P<.05) ob/ob groups (Figure 1(a)). In addition, leptin decreased (P<.01) the high concentrations of MDA measured in the gastrocnemius muscle of control ob/ob mice, while this effect was not observed in the pair-fed group (Figure 1(b)). Serum and gastrocnemius TBARS levels were positively associated with body weight, FFA, insulin, and the HOMA index. Oppositely, TBARS levels were negatively associated with adiponectin and the QUICKI index both in serum and muscle. Importantly, a high positive relation were found between serum and gastrocnemius concentrations of TBARS (ρ=0.63, P=.003) (Table 3).Table 3
Bivariate analysis of the correlations between TBARS concentrations in serum and the gastrocnemius muscle with anthropometric and biochemical variables in wild type andob/ob mice.
SerumTBARSGastrocnemiusTBARSρPρPBody weight0.57.0090.46.040Glucose0.44.0550.38.103FFA0.54.0150.59.007Glycerol0.49<.0010.44.053TG0.44.0540.44.050Insulin0.49.0270.52.020Adiponectin-0.51.022-0.53.016QUICKI-0.48.031-0.48.033HOMA0.53.0190.51.025Values are Spearman's correlation coefficients (ρ) and associated P values. TBARS: thiobarbituric acid reactive substances. FFA: free fatty acids. TG: triglycerides. HOMA: homeostasis model assessment. QUICKI: quantitative insulin sensitivity check index.Leptin reduces TBARS concentrations inob/ob mice. Thiobarbituric acid reactive substances (TBARS) presented as concentrations of malondialdehyde (MDA μM) in serum (a) and gastrocnemius muscle (MDA μM/mg prot) (b) of wild type (open), control ob/ob (closed), pair-fed ob/ob (gray) and leptin-treated ob/ob (striped) mice (n=5 per group). Data are expressed as mean±SEM. *P<.05 and **P<.01 by Kruskal-Wallis followed by Mann Whitney’s U test.
(a)(b)


### 3.2. Leptin Induces Changes in Gene Expression—Effect of Leptin on Genes Invoved in Oxidative Stress and Inflammation

Differential gene expression profiles in gastrocnemius muscle of wild type andob/obgroups were compared by microarray analysis. Only genes whose mRNA levels were changed 1.5-fold or higher and identified as significantly changed by statistical analysis were designated as differentially expressed genes. Applying these criteria, microarray data showed that 7,582 genes were differentially expressed by leptin deficiency and leptin administration in ob/ob mice. In particular, leptin deficiency altered the expression of 1,127 genes between wild type and control ob/ob mice. Of these, 580 were upregulated and 547 were downregulated in ob/ob mice. Leptin treatment modified the expression of 1,546 genes in ob/ob mice, upregulating 512 and repressing 1,034. In addition, leptin repressed 736 genes that were upregulated in gastrocnemius muscle of control ob/ob and increased the transcript levels of 846 downregulated genes. Functional enrichment analysis using GeneOntology and KEGG databases revealed that the set of genes with altered expression levels induced by leptin deficiency and administration represents a broad spectrum of biological processes. However, for the purpose of the present paper we focused on the effects of leptin on the set of genes encoding proteins involved in oxidative stress and inflammation. Table 4 shows that leptin deficiency and leptin administration altered the expression of a large number of genes involved in oxidative stress and inflammation. The biological processes mainly affected between control ob/ob mice and wild types included “response to oxidative stress” (P=.0006), “response to stress” (P=.0031) and “acute-phase response” (P=.023). Furthermore, several processes regulating proliferation, differentiation, and activity of lymphocytes were also significantly affected by leptin deficiency. Importantly, comparison of leptin-treated and control ob/ob groups showed that leptin administration altered the expression of genes implicated in the “positive regulation of lymphocyte activation” (P=.0003), “positive regulation of immune response” (P=.0032) and “response to stress” (P=.0187), as well as genes involved in the “chaperone cofactor dependent protein folding” (P=.0023).Table 4
Biological processes according to Gene Ontology (GO) and number of genes altered by leptin deficiency, leptin administration, and pair-feeding in the gastrocnemius muscle of wild type andob/ob mice.
CategoryGenes in Categorywild typevs ob/obob/ob vs leptinleptinvs pair-fedAltered genesP valueAltered genesP valueAltered genesP valueGO:6950: response to stress115661.0031469.018722.0757GO:6952: defense response101043.18247.510333.83e-6GO:6955: immune response83536.18645.165335.53e-8GO:45321: immune cell activation2309.47513.2706.0974GO:46649: lymphocyte activation2089.35913.1706.0673GO:6954: inflammatory response1994.9384.9842.7590GO:50776: regulation of immune response1489.09712.04268.00102GO:6959: humoral immune response1237.1698.2114.0891GO:42110: T cell activation1125.3967.2635.0191GO:30098: lymphocyte differentiation1078.04418.1234.0597GO:42113: B cell activation1013.7247.1883.1610GO:6800: oxygen and reactive oxygen species metabolism9211.000567.1357.00027GO:50778: positive regulation of immune response917.050811.003283.6e-5GO:51249: regulation of lymphocyte activation897.04610.008085.0076GO:19882: antigen presentation819.00299.012581.53e-5GO:31098: stress-activated protein kinase signaling pathway808.009215.3131.6690GO:30333: antigen processing7811.00013135.65e-581.16e-5GO:7254: JNK cascade758.006294.4611.6450GO:46651: lymphocyte proliferation672.7125.1992.2340GO:6979: response to oxidative stress659.00067.030372.99e-5GO:50863: regulation of T cell activation625.07796.06675.0016GO:7249: I-kappaB kinase/NF-kappaB cascade612.6633.5423.0512GO:51251: positive regulation of lymphocyte activation586.019610.00035.00118GO:30217: T cell differentiation545.04816.03804.00638GO:9266: response to temperature stimulus54124.78e-7137.96e-71.5260GO:30183: B cell differentiation502.5543.4102.1500GO:50670: regulation of lymphocyte proliferation462.5093.3601.4700GO:50864: regulation of B cell activation462.5095.06062.1310GO:42087: cell-mediated immune response441.8091.8762.1220GO:50777: negative regulation of immune response433.2102.5991.4480GO:50870: positive regulation of T cell activation435.02036.01375.000294GO:42088: T-helper 1 type immune response411.7861.8572.1080GO:9408: response to heat4091.17e-5121.54e-71.4240GO:45619: regulation of lymphocyte differentiation366.001865.02424.00144GO:42100: B cell proliferation321.6995.01502.0709GO:19884: antigen presentation, exogenous antigen3191.17e-697.62e-686.81e-9GO:50851: antigen receptor-mediated signaling pathway301.6763.1601.3390GO:50871: positive regulation of B cell activation301.6765.01152.0633GO:51250: negative regulation of lymphocyte activation302.3041.7591.3390GO:50671: positive regulation of lymphocyte proliferation292.2903.1491.3300GO:1909: immune cell mediated cytotoxicity272.2622.3583.00584GO:45580: regulation of T cell differentiation265.002325.006174.00041GO:30888: regulation of B cell proliferation241.5943.09751.2820GO:45621: positive regulation of lymphocyte differentiation224.007885.002883.00323GO:19886: antigen processing, exogenous antigen via MHC class II2192.37e-882.45e-681.98e-10GO:45058: T cell selection202.1671.6133.00244GO:50868: negative regulation of T cell activation201.5281.6131.2410G O:42591: antigen presentation, exogenous antigen via MHC class II1964.42e-56.00015761.47e-7GO:45582: positive regulation of T cell differentiation194.004565.001433.0021GO:1910: regulation of immune cell mediated cytotoxicity182.1412.2023.00178GO:19724: B cell mediated immunity181.4911.5741.2200GO:45577: regulation of B cell differentiation161.4521.5322.0198GO:46328: regulation of JNK cascade161.4522.1681.1980GO:30890: positive regulation of B cell proliferation141.4093.02461.1760GO:45060: negative thymic T cell selection141.4091.4851.1760GO:51085: chaperone cofactor dependent protein folding132.08094.002343.00066GO:1912: positive regulation of immune cell mediated cytotoxicity111.3381.4073.00039GO:48002: antigen presentation, peptide antigen1051.45e-554.39e-546.8e-6GO:48005: antigen presentation, exogenous peptide antigen751.33e-654.11e-641.17e-6GO:45620: negative regulation of lymphocyte differentiation62.01841.2481.0794GO:46330: positive regulation of JNK cascade41.1391.1731.0537GO:45581: negative regulation of T cell differentiation21.07231.09051.0272P values reflect the significance of change in prevalence of genes in each category under the leptin deficiency (ob/ob), leptin administration (leptin) and pair-feeding (pair-fed) conditions in ob/ob mice to the expected prevalence of genes in each category. Statistically significant P values are highlighted in bold.Noteworthy, leptin reduced the expression of several genes related to inflammatory conditions. DNA microarray analysis showed that 86 genes encoding proteins related to defense, stress, and inflammatory responses were altered in the gastrocnemius muscle of controlob/ob mice and modified by leptin administration. Leptin reduced the mRNA levels of various isoforms of the family of heat shock proteins (HSPs) (Dnajc16, Dnaja4, Dnajb4, Hspa2, Hspa4, and Hspb7), metallothioneins (Mt2, Mt4), crystallins (Cryab, Crybb1) and RNA binding proteins (RBMs) (Rbm9, Rbm22) in ob/ob mice (Table 5). In addition, histocompatibility 2, complement component factor B H2-Bf and several genes of the acute-phase response or inflammatory processes, such as kallikrein 5 (Klk5), and serine (or cysteine) proteinase inhibitor clade C member 1 (Serpinc1) and clade B member 1a (Serpinb1a), displayed an increased expression in ob/ob mice that was reduced by leptin administration. On the contrary, gene expression of Cryl1, Hsp105, Rbm5, and H2-Aa were enhanced in ob/obmice after treated with leptin. Pair-feeding, which accounts for the decrease in food intake that is independent of the direct action of leptin, altered the expression of 1,960 genes, upregulating 984 while downregulating 976 genes. In the context of a food intake reduction as compared to the simple effect due to the caloric restriction, leptin administration further significantly altered the expression of genes involved in processes encompassing “immune response” (P=5.53e-8) “defense response” (P=3.83e-6), “response to oxidative stress” (P=2.99e-5), “positive regulation of T cell activation” (P=.0003) and “positive regulation of immune cell mediated cytotoxicity” (P=.0004) (Table 4). In particular, the gene array analysis provided evidence for elevated Hspa4, Mt4, Crybb1, and Serpinb8 mRNA levels in the pair-fed group as compared to the leptin-treated ob/ob mice (Table 6). On the contrary, leptin increased the gene expression of H2-Ab1 and H2-Eb1 in ob/ob mice. To confirm the microarray data, the mRNA expression of several representative transcripts was analyzed by Real-Time PCR (Figure 2). In this sense, leptin administration reduced the mRNA levels of the muscle atrophy-related transcription factor forkhead box O1 (Foxo1) and of the E3 ubiquitin-ligases muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1) in leptin-treated ob/ob mice, while no effect of leptin was evidenced on the mRNA levels of the transcriptional coactivator peroxisome proliferator-activated receptor-γ coactivator-1α  (Pgc1α). The expression of the selected genes was concordant with that of the microarray.Table 5
Genes involved in oxidative stress and inflammatory responses altered by leptin in the gastrocnemius muscle ofob/ob mice.
GeneBank NumberGene SymbolGene NameFold changeRatioob/obleptinGenes downregulated by leptinNM_009804CatCatalase1.471.130.77NM_007705CirbpCold inducible RNA binding protein1.681.140.68NM_009964CryabCrystallin,αB1.321.150.87NM_023695Crybb1Crystallin,βB12.211.390.63NM_023646Dnaja3DnaJ (Hsp40) homolog, subfamily A, member 30.950.640.67NM_021422Dnaja4Heat shock protein, DNAJ-like 40.880.300.34NM_018808Dnajb1DnaJ (Hsp40) homolog, subfamily B, member 10.440.330.74NM_026400Dnajb11DnaJ (Hsp40) homolog, subfamily B, member 111.110.930.84NM_027287Dnajb4DnaJ (Hsp40) homolog, subfamily B, member 41.090.600.55NM_019874Dnajb5DnaJ (Hsp40) homolog, subfamily B, member 51.030.730.72NM_011847Dnajb6DnaJ (Hsp40) homolog, subfamily B, member 6 isoform c0.700.470.67NM_013760Dnajb9DnaJ (Hsp40) homolog, subfamily B, member 90.620.390.63NM_007869Dnajc1DnaJ (Hsp40) homolog, subfamily C, member 10.820.520.63NM_028873Dnajc14DnaJ (Hsp40) homolog, subfamily C, member 141.120.870.77NM_172338Dnajc16DnaJ (Hsp40) homolog, subfamily C, member 161.150.660.57NM_009584Dnajc2DnaJ (Hsp40) homolog, subfamily C, member 21.010.820.81NM_008929Dnajc3DnaJ (Hsp40) homolog, subfamily C, member 3B1.020.830.82NM_016775Dnajc5DnaJ (Hsp40) homolog, subfamily C, member 50.740.500.67NM_010344GsrGlutathione reductase 11.170.710.61NM_008180GssGlutathione synthetase1.130.880.78NM_010357Gsta4Glutathione S-transferase,α41.501.460.97NM_010362Gsto1Glutathione S-transferaseο 11.421.150.81NM_008198H2-BfHistocompatibility 2, complement component factor B2.001.440.72NM_013558Hspa1lHeat shock 70kDa protein 1-like1.601.040.65NM_008301Hspa2Heat shock protein 21.490.980.65NM_008300Hspa4Heat shock protein 40.920.300.32NM_031165Hspa8Heat shock protein 80.910.570.62NM_010481Hspa9aHeat shock protein 91.030.880.86NM_024441Hspb2Heat shock protein 21.451.210.83NM_019960Hspb3Heat shock protein 31.661.270.77NM_013868Hspb7Heat shock protein family, member 71.830.350.19NM_008302HspcbHeat shock protein 1,β0.860.690.80NM_008416JunbJun-B oncogene0.590.360.61NM_010592Jund1Jun D proto-oncogene1.490.940.63NM_008456Klk5Kallikrein 52.231.430.64NM_026346MAFbxMuscle atrophy F box0.650.430.67NM_008209Mr1Histocompatibility-2 complex class 1-like1.190.980.82NM_008630Mt2Metallothionein 21.110.500.46NM_008631Mt4Metallothionein 41.271.030.81NM_008872PlatPlasminogen activator, tissue1.561.120.72NM_029397Rbm12RNA binding motif protein 121.401.030.74NM_026453Rbm13RNA binding motif protein 131.010.870.86NM_026434Rbm18RNA binding motif protein 180.940.590.63BC080205Rbm22RNA binding motif protein 221.140.750.66BC040811Rbm28Rbm28 protein0.690.490.71NM_172762Rbm34RNA binding motif protein 341.010.670.66NM_009032Rbm4RNA binding motif protein 41.040.810.78NM_148930Rbm5RNA binding motif protein 50.690.630.91NM_144948Rbm7RNA binding motif protein 70.810.740.91NM_025875Rbm8aRNA binding motif protein 8a0.910.690.76NM_175387Rbm9RNA binding motif protein 9 isoform 21.960.460.23NM_025429Serpinb1aSerine (or cysteine) proteinase inhibitor, clade B, member 1a2.732.090.77NM_080844Serpinc1Serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 14.981.930.39NM_008871Serpine1Serine (or cysteine) proteinase inhibitor, clade E, member 12.120.970.46NM_011340Serpinf1Serine (or cysteine) proteinase inhibitor, clade F, member 12.431.500.62NM_009776Serping1Serine (or cysteine) proteinase inhibitor, clade G, member 11.411.150.81NM_009776Serping1Serine (or cysteine) proteinase inhibitor, clade G, member 11.411.150.81NM_013749Tnfrsf12aTumor necrosis factor receptor superfamily, member 12a0.780.290.37Genes upregulated by leptinNM_030004Cryl1Crystallinλ11.251.721.38NM_016669CrymCrystallinμ1.371.641.19NM_133679Cryzl1Crystallin,ζ(quinone reductase)-like 11.101.281.16NM_008161Gpx3Glutathione peroxidase 3 isoform 20.470.541.15NM_024198Gpx7Glutathione peroxidase 71.001.341.33NM_010359Gstm3Glutathione S-transferase,μ31.061.231.17NM_010360Gstm5Glutathione S-transferase,μ51.091.391.27NM_013541Gstp1Glutathione S-transferase,π10.871.041.20NM_010361Gstt2Glutathione S-transferase,θ21.211.701.40NM_133994Gstt3Glutathione S-transferase,θ31.531.691.11NM_010363Gstz1Glutathione transferase zeta 1 (maleylacetoacetate isomerase)1.131.241.10NM_010378H2-AaHistocompatibility 2, class II antigen A,α0.461.262.76NM_010379H2-Ab1Histocompatibility 2, class II antigen A,β10.371.042.84NM_010382H2-Eb1Histocompatibility 2, class II antigen Eβ0.431.032.40NM_010395H2-T10Histocompatibility 2, T region locus 101.111.411.27NM_013559Hsp105Heat shock protein 1050.410.731.79NM_008303Hspe1Heat shock protein 1 (chaperonin 10)0.670.981.48AK_052911MuRF1M muscle RING finger 10.200.281.43XM_131139Rbm15RNA binding motif protein 150.811.341.66NM_197993Rbm21RNA binding motif protein 210.670.731.08BC029079Rbm26Rbm26 protein0.751.191.59AK087759Rbm27RNA binding motif protein 270.881.191.36NM_148930Rbm5RNA binding motif protein 50.771.181.55NM_011251Rbm6RNA binding motif protein 6 isoform a0.800.971.21NM_207105Rmcs1histocompatibility 2, class II antigen A,β10.380.892.37NM_011454Serpinb6bSerine (or cysteine) proteinase inhibitor, clade B, member 6b1.061.231.16NM_009825Serpinh1Serine (or cysteine) proteinase inhibitor, clade H, member 10.650.991.53NM_145533SmoxSpermine oxidase0.411.233.00AK080908Sod1Superoxide dismutase0.580.621.07NM_011723XdhXanthine dehydrogenase0.681.011.47Differential expression of genes is indicated as fold changes with respect to the wild type group presenting only the genes which were significantly different (P<.05) between the leptin-treated and the ob/ob groups. Ratio: fold change value for leptin-treated between the ob/ob groups.Table 6
Genes involved in oxidative stress and inflammatory responses altered by leptin in gastrocnemius muscle ofob/obmice independently of food intake restriction.
GeneBank NumberGene symbolGene nameFold changeGenes downregulated by leptinNM_023695Crybb1Crystallin,βB10.51NM_021422Dnaja4Heat shock protein, DNAJ-like 40.63NM_019739Foxo1Forkhead box O10.34NM_008300Hspa4Heat shock protein 40.64NM_013868Hspb7Heat shock protein family, member 70.34NM_010592Jund1Jun D proto-oncogene0.50NM_008456Klk5Kallikrein 50.46NM_008491Lcn2Lipocalin 20.34NM_008631Mt4Metallothionein 40.63NM_026346MAFbxMuscle atrophy F box0.37AK_052911MuRF1M muscle RING finger 10.29NM_011459Serpinb8Serine (or cysteine) proteinase inhibitor, clade B, member 80.38NM_011459Serpinb8Serine (or cysteine) proteinase inhibitor, clade B, member 80.59NM_008871Serpine1Serine (or cysteine) proteinase inhibitor, clade E, member 10.42Genes upregulated by leptinNM_009735B2mβ-2-microglobulin1.92NM_010361Gstt2Glutathione S-transferase,θ21.94NM_010379H2-Ab1Histocompatibility 2, class II antigen A,β14.72NM_010379H2-Ab1Histocompatibility 2, class II antigen A,β13.66NM_010386H2-DMaHistocompatibility 2, class II, locus Dma2.35NM_010387H2-DMb1Histocompatibility 2, class II, locus Mb13.31NM_010382H2-Eb1Histocompatibility 2, class II antigen Eβ4.65NM_013559Hsp105Heat shock protein 1051,79AK220167Hspa4MKIAA4025 protein1,59NM_207105Rmcs1Histocompatibility 2, class II antigen A,β14.24NM_207105Rmcs1Histocompatibility 2, class II antigen A,β14.17NM_009255Serpine2Serine (or cysteine) proteinase inhibitor, clade E, member 21.53NM_009825Serpinh1Serine (or cysteine) proteinase inhibitor, clade H, member 12.21NM_145533SmoxSpermine oxidase4.67Differential expression of genes is indicated as fold changes presenting only the genes which were significantly different (P<.05) between the leptin-treated and the pair-fed ob/ob groups.Real-Time PCR analysis of peroxisome proliferator-activated receptor coactivator 1α  (Pgc1a), forkhead box class O1 (Foxo1), muscle atrophy F box (MAFbx) and muscle RING finger 1 (MuRF1) in gastrocnemius muscle of wild type (open), control ob/ob (closed), pair-fed ob/ob (gray) and leptin-treated ob/ob (striped) mice (n=5 per group). Data are presented as mean±SEM of the ratio between gene expression and 18S rRNA. *P<.05 and **P<.01 by Kruskal-Wallis followed by Mann Whitney’s U test.
(a)(b)(c)(d)


## 3.1. Leptin Treatment Improves the Metabolic Profile of ob/ob Mice

The morphological and biochemical characteristics of wild type andob/ob mice are reported in Table 2. As expected, leptin treatment corrected the obese and diabetic phenotype of ob/ob mice. Body weight was significantly higher (P<.01) in the control ob/ob group as compared to wild type mice. Leptin-treated mice exhibited a decreased body weight (P<.01) as compared to control and pair-fed ob/ob animals. Importantly, leptin treatment normalized body weight of ob/ob mice as compared to wild type (P=.690). In addition, the gastrocnemius of control ob/ob mice exhibited a lower (P<.01) muscle weight than that of wild type mice and it was increased (P<.01) by leptin administration in comparison with that of control and pair-fed ob/ob rodents. As depicted in Table 2, higher fasting glucose (P<.05) and insulin (P<.01) concentrations were observed in the control ob/ob mice compared to wild types. Although no differences in glucose concentrations were observed in pair-fed as compared to leptin-treated ob/ob mice, higher serum insulin concentrations (P<.05) were detected in the pair-fed animals than in the leptin-treated ob/ob group. Furthermore, leptin administration normalized both the glucose and insulin levels in ob/ob mice compared to wild types. These data suggest that leptin increases the insulin sensitivity in peripheral tissues, as evidenced by the lower HOMA and higher QUICKI indices (P<.01) in the leptin-treated in comparison with the control ob/ob animals. Serum glycerol was markedly increased (P<.05) in the control ob/ob mice, while FFA and TG levels remained unchanged as compared to wild type mice. Interestingly, leptin not only decreased circulating concentrations of FFA (P<.05) and glycerol (P<.01) levels as compared to control ob/obmice, but also FFA (P<.01), glycerol (P<.01) and TG (P<.05) concentrations as compared to pair-fed mice. Leptin administration to ob/ob mice reduced serum glycerol concentrations (P=.032) and tended to decrease FFA (P=.095) as compared to wild types. Furthermore, leptin treatment increased the low concentrations of adiponectin of ob/ob mice, but the differences fell out of statistical significance (P=.095).Table 2
Total body and skeletal muscle weights and biochemical characteristics of wild type andob/ob mice.
wild typecontrolob/obpair-fedob/obleptin-treatedob/obBody weight (g)25.6±0.347.8±4.9b35.7±0.724.7±1.2d,fGastrocnemius (mg)142.9±3.490.7±10.0b68.5±1.6104.9±2.6b,fGastrocnemius (mg/g)5.59±0.121.91±0.11b1.92±0.074.28±0.15b,d,fGlucose (mg/dL)149±42430±59a160±24d178±29dFFA (mmol/L)1.62±0.491.61±0.301.65±0.120.78±0.13c,fGlycerol (mmol/L)42.8±6.781.6±19.6a39.6±4.9c12.3±4.7a,d,fTG (mg/dL)122±18169±32151±1086±17eInsulin (ng/mL)0.42±0.098.60±1.51b2.40±0.68c0.47±0.09d,eAdiponectin (μg/mL)30.2±3.028.3±5.439.1±1.840.2±3.0Leptin (ng/mL)1.36±0.42UDUD3.48±1.02HOMA4.3±1.8202.4±33.8b25.8±10.4d5.12±1.1dQUICKI0.333±0.0230.205±0.003b0.263±0.015d0.311±0.016dData aremean±SEM (n=5 per group). Differences between groups were analyzed by Kruskal-Wallis followed by Mann Whitney’s U test. aP<.05 and bP<.01versus wild type. cP<.05 and dP<.01   versus  ob/ob. eP<.05 and fP<.01versus pair-fed ob/ob. FFA: free fatty acids. TG: triglycerides. UD: undetectable. HOMA: homeostasis model assessment. QUICKI: quantitative insulin sensitivity check index.Controlob/ob mice exhibited significantly higher serum TBARS than wild type littermates (P<.01), which were significantly reduced after leptin administration as compared to the control (P<.01) and pair-fed (P<.05) ob/ob groups (Figure 1(a)). In addition, leptin decreased (P<.01) the high concentrations of MDA measured in the gastrocnemius muscle of control ob/ob mice, while this effect was not observed in the pair-fed group (Figure 1(b)). Serum and gastrocnemius TBARS levels were positively associated with body weight, FFA, insulin, and the HOMA index. Oppositely, TBARS levels were negatively associated with adiponectin and the QUICKI index both in serum and muscle. Importantly, a high positive relation were found between serum and gastrocnemius concentrations of TBARS (ρ=0.63, P=.003) (Table 3).Table 3
Bivariate analysis of the correlations between TBARS concentrations in serum and the gastrocnemius muscle with anthropometric and biochemical variables in wild type andob/ob mice.
SerumTBARSGastrocnemiusTBARSρPρPBody weight0.57.0090.46.040Glucose0.44.0550.38.103FFA0.54.0150.59.007Glycerol0.49<.0010.44.053TG0.44.0540.44.050Insulin0.49.0270.52.020Adiponectin-0.51.022-0.53.016QUICKI-0.48.031-0.48.033HOMA0.53.0190.51.025Values are Spearman's correlation coefficients (ρ) and associated P values. TBARS: thiobarbituric acid reactive substances. FFA: free fatty acids. TG: triglycerides. HOMA: homeostasis model assessment. QUICKI: quantitative insulin sensitivity check index.Leptin reduces TBARS concentrations inob/ob mice. Thiobarbituric acid reactive substances (TBARS) presented as concentrations of malondialdehyde (MDA μM) in serum (a) and gastrocnemius muscle (MDA μM/mg prot) (b) of wild type (open), control ob/ob (closed), pair-fed ob/ob (gray) and leptin-treated ob/ob (striped) mice (n=5 per group). Data are expressed as mean±SEM. *P<.05 and **P<.01 by Kruskal-Wallis followed by Mann Whitney’s U test.
(a)(b)


## 3.2. Leptin Induces Changes in Gene Expression—Effect of Leptin on Genes Invoved in Oxidative Stress and Inflammation

Differential gene expression profiles in gastrocnemius muscle of wild type andob/obgroups were compared by microarray analysis. Only genes whose mRNA levels were changed 1.5-fold or higher and identified as significantly changed by statistical analysis were designated as differentially expressed genes. Applying these criteria, microarray data showed that 7,582 genes were differentially expressed by leptin deficiency and leptin administration in ob/ob mice. In particular, leptin deficiency altered the expression of 1,127 genes between wild type and control ob/ob mice. Of these, 580 were upregulated and 547 were downregulated in ob/ob mice. Leptin treatment modified the expression of 1,546 genes in ob/ob mice, upregulating 512 and repressing 1,034. In addition, leptin repressed 736 genes that were upregulated in gastrocnemius muscle of control ob/ob and increased the transcript levels of 846 downregulated genes. Functional enrichment analysis using GeneOntology and KEGG databases revealed that the set of genes with altered expression levels induced by leptin deficiency and administration represents a broad spectrum of biological processes. However, for the purpose of the present paper we focused on the effects of leptin on the set of genes encoding proteins involved in oxidative stress and inflammation. Table 4 shows that leptin deficiency and leptin administration altered the expression of a large number of genes involved in oxidative stress and inflammation. The biological processes mainly affected between control ob/ob mice and wild types included “response to oxidative stress” (P=.0006), “response to stress” (P=.0031) and “acute-phase response” (P=.023). Furthermore, several processes regulating proliferation, differentiation, and activity of lymphocytes were also significantly affected by leptin deficiency. Importantly, comparison of leptin-treated and control ob/ob groups showed that leptin administration altered the expression of genes implicated in the “positive regulation of lymphocyte activation” (P=.0003), “positive regulation of immune response” (P=.0032) and “response to stress” (P=.0187), as well as genes involved in the “chaperone cofactor dependent protein folding” (P=.0023).Table 4
Biological processes according to Gene Ontology (GO) and number of genes altered by leptin deficiency, leptin administration, and pair-feeding in the gastrocnemius muscle of wild type andob/ob mice.
CategoryGenes in Categorywild typevs ob/obob/ob vs leptinleptinvs pair-fedAltered genesP valueAltered genesP valueAltered genesP valueGO:6950: response to stress115661.0031469.018722.0757GO:6952: defense response101043.18247.510333.83e-6GO:6955: immune response83536.18645.165335.53e-8GO:45321: immune cell activation2309.47513.2706.0974GO:46649: lymphocyte activation2089.35913.1706.0673GO:6954: inflammatory response1994.9384.9842.7590GO:50776: regulation of immune response1489.09712.04268.00102GO:6959: humoral immune response1237.1698.2114.0891GO:42110: T cell activation1125.3967.2635.0191GO:30098: lymphocyte differentiation1078.04418.1234.0597GO:42113: B cell activation1013.7247.1883.1610GO:6800: oxygen and reactive oxygen species metabolism9211.000567.1357.00027GO:50778: positive regulation of immune response917.050811.003283.6e-5GO:51249: regulation of lymphocyte activation897.04610.008085.0076GO:19882: antigen presentation819.00299.012581.53e-5GO:31098: stress-activated protein kinase signaling pathway808.009215.3131.6690GO:30333: antigen processing7811.00013135.65e-581.16e-5GO:7254: JNK cascade758.006294.4611.6450GO:46651: lymphocyte proliferation672.7125.1992.2340GO:6979: response to oxidative stress659.00067.030372.99e-5GO:50863: regulation of T cell activation625.07796.06675.0016GO:7249: I-kappaB kinase/NF-kappaB cascade612.6633.5423.0512GO:51251: positive regulation of lymphocyte activation586.019610.00035.00118GO:30217: T cell differentiation545.04816.03804.00638GO:9266: response to temperature stimulus54124.78e-7137.96e-71.5260GO:30183: B cell differentiation502.5543.4102.1500GO:50670: regulation of lymphocyte proliferation462.5093.3601.4700GO:50864: regulation of B cell activation462.5095.06062.1310GO:42087: cell-mediated immune response441.8091.8762.1220GO:50777: negative regulation of immune response433.2102.5991.4480GO:50870: positive regulation of T cell activation435.02036.01375.000294GO:42088: T-helper 1 type immune response411.7861.8572.1080GO:9408: response to heat4091.17e-5121.54e-71.4240GO:45619: regulation of lymphocyte differentiation366.001865.02424.00144GO:42100: B cell proliferation321.6995.01502.0709GO:19884: antigen presentation, exogenous antigen3191.17e-697.62e-686.81e-9GO:50851: antigen receptor-mediated signaling pathway301.6763.1601.3390GO:50871: positive regulation of B cell activation301.6765.01152.0633GO:51250: negative regulation of lymphocyte activation302.3041.7591.3390GO:50671: positive regulation of lymphocyte proliferation292.2903.1491.3300GO:1909: immune cell mediated cytotoxicity272.2622.3583.00584GO:45580: regulation of T cell differentiation265.002325.006174.00041GO:30888: regulation of B cell proliferation241.5943.09751.2820GO:45621: positive regulation of lymphocyte differentiation224.007885.002883.00323GO:19886: antigen processing, exogenous antigen via MHC class II2192.37e-882.45e-681.98e-10GO:45058: T cell selection202.1671.6133.00244GO:50868: negative regulation of T cell activation201.5281.6131.2410G O:42591: antigen presentation, exogenous antigen via MHC class II1964.42e-56.00015761.47e-7GO:45582: positive regulation of T cell differentiation194.004565.001433.0021GO:1910: regulation of immune cell mediated cytotoxicity182.1412.2023.00178GO:19724: B cell mediated immunity181.4911.5741.2200GO:45577: regulation of B cell differentiation161.4521.5322.0198GO:46328: regulation of JNK cascade161.4522.1681.1980GO:30890: positive regulation of B cell proliferation141.4093.02461.1760GO:45060: negative thymic T cell selection141.4091.4851.1760GO:51085: chaperone cofactor dependent protein folding132.08094.002343.00066GO:1912: positive regulation of immune cell mediated cytotoxicity111.3381.4073.00039GO:48002: antigen presentation, peptide antigen1051.45e-554.39e-546.8e-6GO:48005: antigen presentation, exogenous peptide antigen751.33e-654.11e-641.17e-6GO:45620: negative regulation of lymphocyte differentiation62.01841.2481.0794GO:46330: positive regulation of JNK cascade41.1391.1731.0537GO:45581: negative regulation of T cell differentiation21.07231.09051.0272P values reflect the significance of change in prevalence of genes in each category under the leptin deficiency (ob/ob), leptin administration (leptin) and pair-feeding (pair-fed) conditions in ob/ob mice to the expected prevalence of genes in each category. Statistically significant P values are highlighted in bold.Noteworthy, leptin reduced the expression of several genes related to inflammatory conditions. DNA microarray analysis showed that 86 genes encoding proteins related to defense, stress, and inflammatory responses were altered in the gastrocnemius muscle of controlob/ob mice and modified by leptin administration. Leptin reduced the mRNA levels of various isoforms of the family of heat shock proteins (HSPs) (Dnajc16, Dnaja4, Dnajb4, Hspa2, Hspa4, and Hspb7), metallothioneins (Mt2, Mt4), crystallins (Cryab, Crybb1) and RNA binding proteins (RBMs) (Rbm9, Rbm22) in ob/ob mice (Table 5). In addition, histocompatibility 2, complement component factor B H2-Bf and several genes of the acute-phase response or inflammatory processes, such as kallikrein 5 (Klk5), and serine (or cysteine) proteinase inhibitor clade C member 1 (Serpinc1) and clade B member 1a (Serpinb1a), displayed an increased expression in ob/ob mice that was reduced by leptin administration. On the contrary, gene expression of Cryl1, Hsp105, Rbm5, and H2-Aa were enhanced in ob/obmice after treated with leptin. Pair-feeding, which accounts for the decrease in food intake that is independent of the direct action of leptin, altered the expression of 1,960 genes, upregulating 984 while downregulating 976 genes. In the context of a food intake reduction as compared to the simple effect due to the caloric restriction, leptin administration further significantly altered the expression of genes involved in processes encompassing “immune response” (P=5.53e-8) “defense response” (P=3.83e-6), “response to oxidative stress” (P=2.99e-5), “positive regulation of T cell activation” (P=.0003) and “positive regulation of immune cell mediated cytotoxicity” (P=.0004) (Table 4). In particular, the gene array analysis provided evidence for elevated Hspa4, Mt4, Crybb1, and Serpinb8 mRNA levels in the pair-fed group as compared to the leptin-treated ob/ob mice (Table 6). On the contrary, leptin increased the gene expression of H2-Ab1 and H2-Eb1 in ob/ob mice. To confirm the microarray data, the mRNA expression of several representative transcripts was analyzed by Real-Time PCR (Figure 2). In this sense, leptin administration reduced the mRNA levels of the muscle atrophy-related transcription factor forkhead box O1 (Foxo1) and of the E3 ubiquitin-ligases muscle atrophy F-box (MAFbx) and muscle RING finger 1 (MuRF1) in leptin-treated ob/ob mice, while no effect of leptin was evidenced on the mRNA levels of the transcriptional coactivator peroxisome proliferator-activated receptor-γ coactivator-1α  (Pgc1α). The expression of the selected genes was concordant with that of the microarray.Table 5
Genes involved in oxidative stress and inflammatory responses altered by leptin in the gastrocnemius muscle ofob/ob mice.
GeneBank NumberGene SymbolGene NameFold changeRatioob/obleptinGenes downregulated by leptinNM_009804CatCatalase1.471.130.77NM_007705CirbpCold inducible RNA binding protein1.681.140.68NM_009964CryabCrystallin,αB1.321.150.87NM_023695Crybb1Crystallin,βB12.211.390.63NM_023646Dnaja3DnaJ (Hsp40) homolog, subfamily A, member 30.950.640.67NM_021422Dnaja4Heat shock protein, DNAJ-like 40.880.300.34NM_018808Dnajb1DnaJ (Hsp40) homolog, subfamily B, member 10.440.330.74NM_026400Dnajb11DnaJ (Hsp40) homolog, subfamily B, member 111.110.930.84NM_027287Dnajb4DnaJ (Hsp40) homolog, subfamily B, member 41.090.600.55NM_019874Dnajb5DnaJ (Hsp40) homolog, subfamily B, member 51.030.730.72NM_011847Dnajb6DnaJ (Hsp40) homolog, subfamily B, member 6 isoform c0.700.470.67NM_013760Dnajb9DnaJ (Hsp40) homolog, subfamily B, member 90.620.390.63NM_007869Dnajc1DnaJ (Hsp40) homolog, subfamily C, member 10.820.520.63NM_028873Dnajc14DnaJ (Hsp40) homolog, subfamily C, member 141.120.870.77NM_172338Dnajc16DnaJ (Hsp40) homolog, subfamily C, member 161.150.660.57NM_009584Dnajc2DnaJ (Hsp40) homolog, subfamily C, member 21.010.820.81NM_008929Dnajc3DnaJ (Hsp40) homolog, subfamily C, member 3B1.020.830.82NM_016775Dnajc5DnaJ (Hsp40) homolog, subfamily C, member 50.740.500.67NM_010344GsrGlutathione reductase 11.170.710.61NM_008180GssGlutathione synthetase1.130.880.78NM_010357Gsta4Glutathione S-transferase,α41.501.460.97NM_010362Gsto1Glutathione S-transferaseο 11.421.150.81NM_008198H2-BfHistocompatibility 2, complement component factor B2.001.440.72NM_013558Hspa1lHeat shock 70kDa protein 1-like1.601.040.65NM_008301Hspa2Heat shock protein 21.490.980.65NM_008300Hspa4Heat shock protein 40.920.300.32NM_031165Hspa8Heat shock protein 80.910.570.62NM_010481Hspa9aHeat shock protein 91.030.880.86NM_024441Hspb2Heat shock protein 21.451.210.83NM_019960Hspb3Heat shock protein 31.661.270.77NM_013868Hspb7Heat shock protein family, member 71.830.350.19NM_008302HspcbHeat shock protein 1,β0.860.690.80NM_008416JunbJun-B oncogene0.590.360.61NM_010592Jund1Jun D proto-oncogene1.490.940.63NM_008456Klk5Kallikrein 52.231.430.64NM_026346MAFbxMuscle atrophy F box0.650.430.67NM_008209Mr1Histocompatibility-2 complex class 1-like1.190.980.82NM_008630Mt2Metallothionein 21.110.500.46NM_008631Mt4Metallothionein 41.271.030.81NM_008872PlatPlasminogen activator, tissue1.561.120.72NM_029397Rbm12RNA binding motif protein 121.401.030.74NM_026453Rbm13RNA binding motif protein 131.010.870.86NM_026434Rbm18RNA binding motif protein 180.940.590.63BC080205Rbm22RNA binding motif protein 221.140.750.66BC040811Rbm28Rbm28 protein0.690.490.71NM_172762Rbm34RNA binding motif protein 341.010.670.66NM_009032Rbm4RNA binding motif protein 41.040.810.78NM_148930Rbm5RNA binding motif protein 50.690.630.91NM_144948Rbm7RNA binding motif protein 70.810.740.91NM_025875Rbm8aRNA binding motif protein 8a0.910.690.76NM_175387Rbm9RNA binding motif protein 9 isoform 21.960.460.23NM_025429Serpinb1aSerine (or cysteine) proteinase inhibitor, clade B, member 1a2.732.090.77NM_080844Serpinc1Serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 14.981.930.39NM_008871Serpine1Serine (or cysteine) proteinase inhibitor, clade E, member 12.120.970.46NM_011340Serpinf1Serine (or cysteine) proteinase inhibitor, clade F, member 12.431.500.62NM_009776Serping1Serine (or cysteine) proteinase inhibitor, clade G, member 11.411.150.81NM_009776Serping1Serine (or cysteine) proteinase inhibitor, clade G, member 11.411.150.81NM_013749Tnfrsf12aTumor necrosis factor receptor superfamily, member 12a0.780.290.37Genes upregulated by leptinNM_030004Cryl1Crystallinλ11.251.721.38NM_016669CrymCrystallinμ1.371.641.19NM_133679Cryzl1Crystallin,ζ(quinone reductase)-like 11.101.281.16NM_008161Gpx3Glutathione peroxidase 3 isoform 20.470.541.15NM_024198Gpx7Glutathione peroxidase 71.001.341.33NM_010359Gstm3Glutathione S-transferase,μ31.061.231.17NM_010360Gstm5Glutathione S-transferase,μ51.091.391.27NM_013541Gstp1Glutathione S-transferase,π10.871.041.20NM_010361Gstt2Glutathione S-transferase,θ21.211.701.40NM_133994Gstt3Glutathione S-transferase,θ31.531.691.11NM_010363Gstz1Glutathione transferase zeta 1 (maleylacetoacetate isomerase)1.131.241.10NM_010378H2-AaHistocompatibility 2, class II antigen A,α0.461.262.76NM_010379H2-Ab1Histocompatibility 2, class II antigen A,β10.371.042.84NM_010382H2-Eb1Histocompatibility 2, class II antigen Eβ0.431.032.40NM_010395H2-T10Histocompatibility 2, T region locus 101.111.411.27NM_013559Hsp105Heat shock protein 1050.410.731.79NM_008303Hspe1Heat shock protein 1 (chaperonin 10)0.670.981.48AK_052911MuRF1M muscle RING finger 10.200.281.43XM_131139Rbm15RNA binding motif protein 150.811.341.66NM_197993Rbm21RNA binding motif protein 210.670.731.08BC029079Rbm26Rbm26 protein0.751.191.59AK087759Rbm27RNA binding motif protein 270.881.191.36NM_148930Rbm5RNA binding motif protein 50.771.181.55NM_011251Rbm6RNA binding motif protein 6 isoform a0.800.971.21NM_207105Rmcs1histocompatibility 2, class II antigen A,β10.380.892.37NM_011454Serpinb6bSerine (or cysteine) proteinase inhibitor, clade B, member 6b1.061.231.16NM_009825Serpinh1Serine (or cysteine) proteinase inhibitor, clade H, member 10.650.991.53NM_145533SmoxSpermine oxidase0.411.233.00AK080908Sod1Superoxide dismutase0.580.621.07NM_011723XdhXanthine dehydrogenase0.681.011.47Differential expression of genes is indicated as fold changes with respect to the wild type group presenting only the genes which were significantly different (P<.05) between the leptin-treated and the ob/ob groups. Ratio: fold change value for leptin-treated between the ob/ob groups.Table 6
Genes involved in oxidative stress and inflammatory responses altered by leptin in gastrocnemius muscle ofob/obmice independently of food intake restriction.
GeneBank NumberGene symbolGene nameFold changeGenes downregulated by leptinNM_023695Crybb1Crystallin,βB10.51NM_021422Dnaja4Heat shock protein, DNAJ-like 40.63NM_019739Foxo1Forkhead box O10.34NM_008300Hspa4Heat shock protein 40.64NM_013868Hspb7Heat shock protein family, member 70.34NM_010592Jund1Jun D proto-oncogene0.50NM_008456Klk5Kallikrein 50.46NM_008491Lcn2Lipocalin 20.34NM_008631Mt4Metallothionein 40.63NM_026346MAFbxMuscle atrophy F box0.37AK_052911MuRF1M muscle RING finger 10.29NM_011459Serpinb8Serine (or cysteine) proteinase inhibitor, clade B, member 80.38NM_011459Serpinb8Serine (or cysteine) proteinase inhibitor, clade B, member 80.59NM_008871Serpine1Serine (or cysteine) proteinase inhibitor, clade E, member 10.42Genes upregulated by leptinNM_009735B2mβ-2-microglobulin1.92NM_010361Gstt2Glutathione S-transferase,θ21.94NM_010379H2-Ab1Histocompatibility 2, class II antigen A,β14.72NM_010379H2-Ab1Histocompatibility 2, class II antigen A,β13.66NM_010386H2-DMaHistocompatibility 2, class II, locus Dma2.35NM_010387H2-DMb1Histocompatibility 2, class II, locus Mb13.31NM_010382H2-Eb1Histocompatibility 2, class II antigen Eβ4.65NM_013559Hsp105Heat shock protein 1051,79AK220167Hspa4MKIAA4025 protein1,59NM_207105Rmcs1Histocompatibility 2, class II antigen A,β14.24NM_207105Rmcs1Histocompatibility 2, class II antigen A,β14.17NM_009255Serpine2Serine (or cysteine) proteinase inhibitor, clade E, member 21.53NM_009825Serpinh1Serine (or cysteine) proteinase inhibitor, clade H, member 12.21NM_145533SmoxSpermine oxidase4.67Differential expression of genes is indicated as fold changes presenting only the genes which were significantly different (P<.05) between the leptin-treated and the pair-fed ob/ob groups.Real-Time PCR analysis of peroxisome proliferator-activated receptor coactivator 1α  (Pgc1a), forkhead box class O1 (Foxo1), muscle atrophy F box (MAFbx) and muscle RING finger 1 (MuRF1) in gastrocnemius muscle of wild type (open), control ob/ob (closed), pair-fed ob/ob (gray) and leptin-treated ob/ob (striped) mice (n=5 per group). Data are presented as mean±SEM of the ratio between gene expression and 18S rRNA. *P<.05 and **P<.01 by Kruskal-Wallis followed by Mann Whitney’s U test.
(a)(b)(c)(d)


## 4. Discussion

Obesity is accompanied by a chronic proinflammatory state associated not only with insulin resistance, but also with muscular atrophy [4, 5]. Our study provides evidence that leptin constitutes a negative regulator of oxidative stress and inflammation in the gastrocnemius, which is a representative skeletal muscle of the whole skeletal musculature. This statement is supported by findings reported herein: (a) leptin deficiency is accompanied by systemic and skeletal muscle oxidative stress, muscle inflammation, and reduced muscle mass; (b) systemic and skeletal muscle oxidative stress, muscle atrophy and inflammation of ob/ob mice are reversed by leptin administration independently of the effects of food intake inhibition. Therefore, leptin is able to prevent the muscle atrophy associated with obese and inflammatory states.Skeletal muscle constitutes an important target for leptin playing a key role on the regulation of lipid and glucose metabolism [20]. Since obese ob/ob mice exhibit an increased oxidative stress and impaired immune response [14, 15] and a reduced skeletal muscle mass [21] compared with their lean littermates, we aimed to identify the genes related to inflammatory processes differentially altered by leptin in the gastrocnemius muscle of obese ob/ob mice. In particular, 86 transcripts encoding inflammation-related proteins were shown to be modified by exogenous leptin administration. However, it has to be taken into account that many of these genes are multifunctional and may have important functions in other biological processes. Among them, leptin repressed the high expression levels of acute-phase reactants and several members of the HSP and RBM families. In addition, confirming a previous study of our group [6], leptin treatment increased the reduced muscle weight of gastrocnemius muscle of ob/ob mice. Taken together, these data suggest that leptin may prevent the obesity-associated inflammatory state and the muscle mass loss related to inflammatory states in leptin-deficient ob/ob mice.Leptin-deficientob/ob and leptin receptor-deficient db/db mice display many abnormalities in the immune response similar to those observed in starved animals and malnourished humans [14, 15, 22]. In this respect, exogenous leptin replacement to ob/ob mice modulates T cell responses in mice and prevents starvation-induced immunosuppression, suggesting that lack of leptin is directly involved in these immune system abnormalities [23, 24]. In agreement with these studies, our findings show that leptin deficiency and administration differentially regulate biological processes related to the immune response as well as the T and B cell differentiation and activation in gastrocnemius muscle of ob/ob mice.Oxidative stress is defined as the imbalanced redox state in which prooxidants overwhelm the antioxidant capacity, resulting in an increased production of reactive oxygen species (ROS), ultimately leading to oxidative damage of cellular macromolecules. The major ROS is the superoxide anion (•O2-). Dismutation of •O2- by superoxide dismutase (SOD) produces hydrogen peroxide (H2O2), a more stable ROS, which, in turn, is converted to water by catalase and glutathione peroxidase (GPx) [25]. Oxidative stress is increased in diabetes [26, 27] with leptin administration reportedly improving insulin sensitivity in normal and diabetic rodents [28–30]. However, the relationship between leptin and oxidative stress has not been clearly exhibited. Leptin stimulates in vitro ROS production by inflammatory cells [31] and endothelial cells [32] and the level of systemic oxidative stress in nonobese animals [33, 34], suggesting a “prooxidative” role of leptin. However, administration of recombinant leptin reduces the oxidative stress induced by a high-fat diet in mice [35]. In this sense, findings of our study show a high oxidative stress in diabetic ob/ob mice, as reflected by increased TBARS concentrations in serum and the gastrocemius muscle. These observations are in agreement with a large number of studies related to increased plasma TBARS or MDA in diabetic rats [36] and humans [37]. Lipid peroxidation is a common index of free radical mediated injury and induction of antioxidant enzyme is a common cellular response [38]. More importantly, leptin administration decreased serum and gastrocnemius TBARS concentrations as compared to control ob/ob mice, with TBARS levels in gastrocnemius muscle from pair-fed ob/ob animals remaining very similar to those of control ob/ob mice. In this sense, from a molecular perspective, our results further show that transcript levels of Sod1, Gpx3 and glutathione S-transferase π 1 Gstp1are downregulated in control ob/ob mice as compared to wild type controls being upregulated after leptin treatment. Furthermore, leptin administration also upregulated Gpx7, glutathione S-transferase μ 5 (Gstm5) and glutathione S-transferase θ 2 (Gstt2). On the contrary, the high expression of catalase (Cat) was repressed by the exogenous injection of leptin to ob/ob mice. These findings are in line with previous observations showing the restoration of the defective antioxidant enzyme activity in plasma of ob/ob mice [39] and humans with a leptin gene mutation [40].Acute-phase reactants have been suggested to contribute to the maintenance of the chronic low-grade inflammation state involved in the progression of obesity and related diseases [41]. Interestingly, our study provides evidence that genes of the acute-phase response were altered in gastrocnemius muscle of ob/ob mice, which were counteracted by exogenous leptin administration. Leptin reduced the elevated gene expression of tissue-type plasminogen activator (Plat) and lipocalin-2 (Lcn2), which are upregulated in many inflammatory conditions [42, 43], including human obesity [44]. In addition, a pivotal role for oxidative stress in the pathogenesis of muscle wasting in disuse and in a variety of pathological conditions is now being widely recognized [45]. A potential link between oxidative stress and muscle atrophy involves the redox regulation of the proteolytic system [46]. Moreover, various inflammatory cytokines induce oxidative stress [47] and muscle atrophy through the activation of the lysosomal [48, 49] and the ubiquitin-proteolysis system [50]. In this context, biological processes related to oxidative stress and inflammatory responses were altered in the gastrocnemius muscle of ob/ob mice and improved following leptin treatment. In spite of the usual  upregulation of the E3 ubiquitin-ligases MAFbx and MuRF1 in most conditions associated with atrophy, their gene expression levels in ob/ob were lower as compared to wild type animals, although no statistically significant differences were observed. Contrarily to what would be expected, leptin administration prevented the increase of both MAFbx and MuRF1 mRNA expression levels induced by pair-feeding in ob/ob mice. A plausible explanation for this surprising finding may relate to the fact that in extreme conditions the energy homeostasis system is overriden whereby leptin is able to inhibit muscular protein degradation associated to food intake reduction. These data are in accordance with a previous study of our group evidencing that leptin replacement inhibits the ubiquitin proteolysis system activity in leptin-deficient mice [6]. Muscle atrophy is associated with increased expression of genes coding for RBM proteins which facilitate the translation, protection, and restoration of native RNA conformations during oxidative stress. It has been suggested that the gene expression of RBM proteins may increase as a compensatory mechanism in response to loss of muscle proteins [51, 52]. Other proteins involved in oxidative stress are metallothioneins, endogenous antioxidants [53] that have been shown to be overexpressed in muscle atrophy in rodents [54–56]. In the present work, we have observed that administration of leptin inhibits the gene expression of several members of the RBM (Rbm9, Rbm22) and metallothioneins (Mt2, Mt4) families in the gastrocnemius of ob/ob mice, suggesting that leptin may modulate the inflammatory and oxidative stress responses and consequently, the muscle loss related to inflammatory states.Genes involved in the chaperone system were also differentially expressed inob/ob mice as compared to wild types and modified by leptin treatment. HSPs represent a family of molecular chaperones induced in response to cellular stress, responsible for maintaining the structure of proteins and contributing to the repair of damaged or malformed proteins in highly oxidative and lipotoxic conditions. As a result, HSPs are considered antiproteolytic proteins [57]. Muscle atrophy is also associated with an increased gene expression of HSPs [58]. In fact, HSPs are repressed in many rat models of skeletal muscle atrophy [54, 59, 60]. HSP70 is constitutively expressed in skeletal muscle, but its levels are increased in response to oxidative stress [61] with the induction of HSP70 expression by hyperthermia and during inactivity attenuating muscle atrophy [62, 63]. In this regard, a recent study has shown that HSP70 prevents muscle atrophy induced by physical inactivity through inhibition of the muscle atrophy-related transcription factor FoxO3a and the expression of MAFbx and MuRF1 [64]. Among the HSPs, HSP70 and αB-crystallin in particular, are considered negative regulators of muscle cell apoptosis [65, 66] and may inhibit the loss of nuclei taking place during muscle atrophy. In addition, ROS induce the activity of FoxO [67] and gene expression of members of the ubiquitin-proteolysis system in myotubes [68]. In this sense, our results provide evidence that leptin inhibits the increased gene expression of different members of the HSPs (Hspb7, Dnajc16, Hspa4, Cryab, and Crybb1) in the gastrocnemius muscle of ob/ob mice. Taken together, the elevated expression of HSPs in the control and pair-fed ob/ob groups suggests a high defense and stress response in these mice. Moreover, induction of HSPs may confer broader health benefits to patients who are insulin resistant or diabetic [69]. In mammals, caloric restriction has been shown to upregulate HSP induction [70, 71], while expression of HSP72 has been found to be low in skeletal muscle of patients with insulin resistance or type 2 diabetes [72, 73]. Figueiredo et al. [74] have recently shown that leptin downregulates HSP70 gene expression in chicken liver and hypothalamus but not in muscle, which was independent of food intake restriction. On the contrary, Bonior et al. [75] reported an increase in HSP60 gene expression in pancreatic cells by leptin.Obesity is accompanied by a chronic proinflammatory state resulting in an increase in circulating cytokines and inflammatory markers. In this regard, inflammation produces metabolic alterations in skeletal muscle with both inflammatory response and insulin resistance being associated with muscle mass loss. Findings of our study provide evidence that systemic and skeletal muscle oxidative stress, muscle atrophy and the elevated expression of genes involved in oxidative stress and inflammation ofob/ob mice are reversed by leptin administration. Taken together, these data thereby support that leptin is able to prevent the muscle atrophy associated with obese and inflammatory states in ob/ob mice. Most obese people develop muscle atrophy in spite of exhibiting high leptin circulating concentrations, which may be explained by the leptin resistance present in these patients. Our paper sheds light on the relation between obesity and the loss of muscle mass associated to inflammatory states suggesting that leptin treatment may be an attractive therapeutic approach to prevent muscle loss associated with inflammatory diseases.



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*Source: 784343-2010-06-30.xml*