# Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C

Authors: Justin F. Shaffer; Peony Wong; Kristina L. Bezold; Samantha P. Harris
Journal: Journal of Biomedicine and Biotechnology
 (2010)

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## Abstract

The N-terminus of cMyBP-C can activate actomyosin interactions in the absence ofCa2+, but it is unclear which domains are necessary. Prior studies suggested that the Pro-Ala rich region of human cMyBP-C activated force in permeabilized human cardiomyocytes, whereas the C1 and M-domains of mouse cMyBP-C activated force in permeabilized rat cardiac trabeculae. Because the amino acid sequence of the P/A region differs between human and mouse cMyBP-C isoforms (46% identity), we investigated whether species-specific differences in the P/A region could account for differences in activating effects. Using chimeric fusion proteins containing combinations of human and mouse C0, Pro-Ala, and C1 domains, we demonstrate here that the human P/A and C1 domains activate actomyosin interactions, whereas the same regions of mouse cMyBP-C are less effective. These results suggest that species-specific differences between homologous cMyBP-C isoforms confer differential effects that could fine-tune cMyBP-C function in hearts of different species.


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## Body

## 1. Introduction

Myosin binding protein-C (MyBP-C) is a regulatory and structural protein associated with the A-bands (thickfilaments) of vertebrate striated muscle sarcomeres. Regulatory effects are mediated in part by the N-terminus of cardiac cMyBP-C, which is made up of modular immunoglobulin-(Ig-) like domains, termed C0 at the N-terminus, followed by domains C1 through C10 (Figure1(a)). Between the C0 and C1 domains there is a sequence of ~50 amino acids that contains a high percentage of proline and alanine residues (referred to as the Pro-Ala rich region (P/A)). Between the C1 and C2 domains there is a stretch of ~100 highly conserved amino acids referred to as MyBP-C motif or M-domain. The M-domain is phosphorylated by β-adrenergic agonists and phosphorylation increases the rate of cross-bridge cycling [1], thereby contributing to increased inotropic responses of the heart [2]. Phosphorylation reduces binding of the M-domain to actin [3] and myosin S2 in vitro [4], but the precise mechanism(s) by which phosphorylation accelerates actomyosin interactions is not well understood.Schematic of full-length cMyBP-C, domain boundaries, and recombinant chimeric mouse and human C0C1 proteins. (a) Schematic of full-length cMyBP-C. The Pro-Ala rich region is denoted by a box between the C0 and C1 domains. (b) Amino acid sequence alignment of mouse and human C0, P/A, and C1 domains. Arrows indicate domain boundaries. Asterisks denote identical residues. (c) Schematic of recombinant chimeric proteins used in this study. Squares represent human domains; circles represent mouse domains.
(a)(b)(c)In efforts to uncover mechanisms by which the N-terminus of cMyBP-C affects cross-bridge cycling several groups have investigated the effects of recombinant proteins containing N-terminal cMyBP-C domains on actomyosin interactions. For instance, Razumova et al. [5] investigated effects of N-terminal domains in in vitro motility assays and found that recombinant proteins containing the C1 through C2 domains of mouse cMyBP-C (referred to as C1C2, inclusive of the M-domain) could activate thin filament motility even in the absence of Ca2+. Incubation of permeabilized rat trabeculae with C1C2 also increased Ca2+ sensitivity of force and increased the rate of tension redevelopment (ktr) [6]. The activating effects of C1C2 were attributed to the combined effects of the C1 and M-domains because these domains together were necessary and sufficient to increase Ca2+ sensitivity of force, whereas other domains including the C0, P/A, and C1 domains had little, if any, effect on force activation. However, in apparent contrast to these results, Herron et al. [7] found that a recombinant protein comprised of the human C0, P/A, and C1 domains (i.e., C0C1) activated tension and increased ktr in permeabilized myocytes from human myocardium, whereas C1C2 (which lacked the P/A region) had no effect. These authors concluded that the P/A rich region was required to activate tension and increase ktr.A potential explanation for the different conclusions reached in the two studies is that species-specific differences are responsible for the different functional effects observed for the mouse versus human proteins. Consistent with this idea, the full-length cMyBP-C protein is quite similar between mouse and human isoforms (89% identity, Table1) and the C1, M, and C2 domains are highly conserved (>90% identity), but domains near the N-terminus show greater sequence divergence with 81% and 46% identity in the C0 domain and P/A regions, respectively (Figure 1(b)). The large difference in sequence homology in the P/A region is primarily due to differences in the content of proline and alanine residues: in the human P/A region, proline and alanine account for 51% of the sequence, while the mouse isoform contains only 28% proline and alanine. Interestingly, a similar trend was noted for a P/A rich sequence in fast skeletal myosin essential light chains (ELC1) where Bicer and Reiser    [8] found that P/A content scaled with mammalian size.Table 1
Sequence identity between domains of human and mouse cMyBP-C.
Protein or DomainNumber of Amino AcidsPercent IdentityMouseHumancMyBP-C1270127489C0C244745184C010410181Pro-Ala465146C111111190M10510791C2818196The purpose of the present study was to determine whether species-specific differences in the P/A rich regions of human and mouse cMyBP-C can account for functional differences in the ability of recombinant proteins from the two species to activate actomyosin interactions. Results show that both the human P/A rich region and the C1 domains can activate actomyosin interactions, whereas the mouse P/A and C1 domains are less effective. These results thus reconcile apparently disparate experimental results and raise the possibility that species-specific variations in cMyBP-C regulatory domains contribute to cross-species variations in cardiac function.


## 2. Methods

### 2.1. Protein Cloning, Expression, and Purification

The recombinant mouse protein mC0C1 (containing the C0, P/A, and C1 domains, Figure1(c)) was subcloned from a full-length mouse cDNA (GenBank gi:3747133), expressed in M15 cells, and purified using Ni-NTA affinity chromatography as previously described [5]. Recombinant human hC0C1 was cloned from a human cDNA obtained by RT-PCR from whole heart human total RNA (Stratagene, La Jolla, CA) using a one-step RT-PCR kit (Invitrogen, Carlsbad, CA). A PCR product encoding hC0C1 was generated using gene specific primers flanking the desired domains (forward primer 5′-GGCCCATATGCCTGAGCCGGGGAAGAAG-3′, reverse primer 5′-GGCCAAGCTTTCATCCGGTGCCCATGGCCTCGTG-3′) and cloned into the pQE-2 expression vector (Qiagen, Valencia, CA) using NdeI and HindIII restriction sites on the upstream and downstream primers, respectively. Expression and purification of hC0C1 were as described previously [5]. Protein concentrations were determined by measuring light absorbance at 280 nm (corrected for turbidity at 310 nm) using extinction coefficients calculated from Swiss Institute for Bioinformatics software [9].


### 2.2. Engineered Chimeric C0C1 Proteins

The In-Fusion PCR Cloning System (Clontech, Mountain View, CA) was used according to manufacturer’s instructions and the protocol by Zhu et al. [10] to create seamless chimeric C0C1 proteins consisting of various combinations of mouse and human C0, P/A, and C1 domains. Boundaries of the C0, P/A, and C1 domains are shown in Figure 1(b) and were as described by Gautel et al.    [11] and as listed for human and mouse cMyBP-C on the Universal Protein Resources Databank (UniProt) [12]. PCR products encoding the mouse and human domains were amplified from mouse and human cMyBP-C cDNA with additional 15 bp flanking sequences at the 5′ and 3′ ends that overlap with the adjacent segment of the construct (Table 2, Figure 1(c)). The pQE-2 expression vector (Qiagen) was digested with NdeI and HindIII and gel-purified. PCR products and the digested pQE-2 vector were mixed at a 1 : 2 : 2 : 2 molar ratio with the In-Fusion enzyme according to manufacturer’s instructions and transformed into Top10 cells (Invitrogen). Correctly ligated plasmids were chosen after selection with carbenicillin and sequences were verified by DNA sequencing at the UC Davis DNA Sequencing Facility. Selected clones were transformed into M15 cells for expression and purification as described previously [5]. Chimeric C0C1 proteins used in this study are shown schematically in Figure 1(c).Table 2
Primers used to create recombinant chimeric C0C1 proteins.
cMyBP-C domainPrimer pairs (upstream/downstream primer)Mouse C05′-ATCACCATCACCATATGCCGGAGCCAGGGAAGAAACCA -3′/5′-AGGGGCCAGCATGGGTTCTGCCTTCTCTGGAGGGGC -3′Mouse P/A5′-TCTGAAGTTGCTCCAGGAGCC -3′/5′-GTCATCAGGGGCTCCCTGATG -3′Mouse C15′-GGAGCCCCCGATGACCCTATTGGCCTCTTTCTGATGCGA -3′/5′-TCAGCTAATTAAGCTTTCATCCAGAACCAATCGCCTCATGGAC -3′Human C05′-ATCACCATCACCATATGCCTGAGCCGGGGAAGAAGC - 3′/5′-TGGAGCAACTTCAGACTCTGCCTTCTCTGCCTCTATGAC -3′Human P/A5′-CCCATGCTGGCCCCTGC -3′/5′-GTCATCGGGGGCTCCAGG -3′Human C15′-GGAGCCCCTGATGACCCCATTGGCCTCTTCGTGATGC -3′/5′-TCAGCTAATTAAGCTTTCATCCGGTGCCCATGGCCT -3′


### 2.3. Native Protein Purification

Heavy meromyosin (HMM) and S1 were prepared from rabbit psoas skeletal myosin viaα-chymotryptic digest as described [13]. Bovine cardiac F-actin, tropomyosin, and troponin were purified from ether powder as described    [14–16]. Thin filaments, comprised of F-actin and regulatory proteins, were reconstituted in AB buffer (in mmol/L: 25 KCl, 25 imidazole (pH 7.4), 4 MgCl2, 1 EGTA, and 1 DTT) by combining at an actin:tropomyosin:troponin ratio of 4 : 1 : 1 for in vitro motility assays and at 7 : 2 : 2 for ATPase assays. The thin filament mix was labeled with rhodamine-phalloidin according to Homsher et al. [17] and used in in vitro motility assays.


### 2.4. Mechanical Force Measurements

Treatment of all animals was in strict accordance with guidelines and protocols established by the University of California Institutional Animal Care and Use Committee. Male Sprague-Dawley rats (200–250 g) were euthanized by intraperitoneal injection of sodium pentobarbital. Hearts were then rapidly excised and right ventricles were dissected in a Ringer’s solution at pH 7.4 (in mmol/L: 100 NaCl, 24 NaHCO3, 2.5 KCl, 1 MgSO4, 1 Na2HPO4, and 1 CaCl2). Trabeculae were permeabilized in situ by incubation of splayed ventricles overnight in a relaxing solution containing 50% glycerol and 1% Triton X-100 (Sigma, St. Louis, Missouri) at 4°C. Individual trabeculae were dissected free from ventricle walls, pinned to the bottom of a sylgar-coated Petri dish, and stored for up to one week in glycerinated relaxing solution at −20°C.Steady state force and rate of tension redevelopment (ktr) measurements were performed as previously described [6]. Briefly, permeabilized trabeculae were mounted between a force transducer (model 403A, Aurora Scientific Inc.) and a torque motor (Model 312-Cl, Aurora Scientific Inc). Sarcomere length was adjusted to ~2.3 μm in relaxing solution and monitored throughout the course of the experiment using an inverted microscope (Olympus IX-71) fitted with a 12-Mega pixel digital camera (Olympus DP70). Relaxing, preactivating, and Ca2+-activating solutions were prepared as previously described using a custom software package    [18, 19]. Solutions were maintained at 0.18 M ionic strength and pH 7.0 at 15°C (in mmol/L: 15 phosphocreatine, 15 EGTA, at least 40 MOPS, 1 free Mg2+, 135 Na+ + K+, 1 DTT, 250 units ml-1 creatine kinase (CK), and 5 ATP). Ca2+ concentration (reported as pCa = −log[Ca2+]) was established by varying amounts of CaCl2. Recombinant proteins were added to relaxing and preactivating solutions by buffer exchange using desalting spin columns (Pierce, Rockford, IL).


### 2.5. In Vitro Motility Assays

In vitro motility assays were performed as described previously [5]. Briefly, HMM was applied to a nitrocellulose-coated coverslip followed by incubation with bovine serum albumin (BSA) to prevent nonspecific adsorption of thin filaments or recombinant proteins to the motility surface. Shredded actin filaments followed by ATP were then added to block nonfunctional myosin heads (dead-heads). Next, rhodamine-phalloidin labeled bovine cardiac thin filaments (4 : 1 : 1 actin : tropomyosin : troponin) were added, followed by a motility buffer containing AB buffer, 2 mM ATP, and an oxygen-scavenging system to limit photo-bleaching. Recombinant cMyBP-C proteins were dialyzed into AB buffer and added to the slide surface in the motility buffer. Motility was viewed using an Olympus IX-71 microscope with an Hg-arc lamp, TRITC filter, and a 100×/1.4 NA oil-immersion objective. Video files were recorded using a Q-Imaging Retiga Exi digital camera and ImageProPlus software. Filament motility was analyzed using custom software developed in LabView and NI Vision Development (National Instruments, Austin, TX) kindly provided by Dr. Michael Regnier (University of Washington).


### 2.6. ATPase Assay

ATPase assays were performed by mixing 0.2μM myosin S1 and 3.5 μM reconstituted bovine cardiac thin filaments (mixed at a 7 : 2 : 2 actin:tropomyosin:troponin ratio) in ATPase buffer (in mmol/L: 10 imidazole (pH 7.4), 2 MgCl2, 1 EGTA, and 1 DTT) with or without 1 μM recombinant cMyBP-C proteins. The addition of 1 mM ATP started the reaction and reactions were quenched at three different time points with stop solution (3.3% SDS, 0.12 M Na-EDTA, pH 7.4). Phosphate production was determined via colorimetric assay as described [20].


### 2.7. Statistical Tests

Data were compared using ANOVA followed by Bonferroni post-hoc comparisons. Significance was considered atP<.05.


## 2.1. Protein Cloning, Expression, and Purification

The recombinant mouse protein mC0C1 (containing the C0, P/A, and C1 domains, Figure1(c)) was subcloned from a full-length mouse cDNA (GenBank gi:3747133), expressed in M15 cells, and purified using Ni-NTA affinity chromatography as previously described [5]. Recombinant human hC0C1 was cloned from a human cDNA obtained by RT-PCR from whole heart human total RNA (Stratagene, La Jolla, CA) using a one-step RT-PCR kit (Invitrogen, Carlsbad, CA). A PCR product encoding hC0C1 was generated using gene specific primers flanking the desired domains (forward primer 5′-GGCCCATATGCCTGAGCCGGGGAAGAAG-3′, reverse primer 5′-GGCCAAGCTTTCATCCGGTGCCCATGGCCTCGTG-3′) and cloned into the pQE-2 expression vector (Qiagen, Valencia, CA) using NdeI and HindIII restriction sites on the upstream and downstream primers, respectively. Expression and purification of hC0C1 were as described previously [5]. Protein concentrations were determined by measuring light absorbance at 280 nm (corrected for turbidity at 310 nm) using extinction coefficients calculated from Swiss Institute for Bioinformatics software [9].


## 2.2. Engineered Chimeric C0C1 Proteins

The In-Fusion PCR Cloning System (Clontech, Mountain View, CA) was used according to manufacturer’s instructions and the protocol by Zhu et al. [10] to create seamless chimeric C0C1 proteins consisting of various combinations of mouse and human C0, P/A, and C1 domains. Boundaries of the C0, P/A, and C1 domains are shown in Figure 1(b) and were as described by Gautel et al.    [11] and as listed for human and mouse cMyBP-C on the Universal Protein Resources Databank (UniProt) [12]. PCR products encoding the mouse and human domains were amplified from mouse and human cMyBP-C cDNA with additional 15 bp flanking sequences at the 5′ and 3′ ends that overlap with the adjacent segment of the construct (Table 2, Figure 1(c)). The pQE-2 expression vector (Qiagen) was digested with NdeI and HindIII and gel-purified. PCR products and the digested pQE-2 vector were mixed at a 1 : 2 : 2 : 2 molar ratio with the In-Fusion enzyme according to manufacturer’s instructions and transformed into Top10 cells (Invitrogen). Correctly ligated plasmids were chosen after selection with carbenicillin and sequences were verified by DNA sequencing at the UC Davis DNA Sequencing Facility. Selected clones were transformed into M15 cells for expression and purification as described previously [5]. Chimeric C0C1 proteins used in this study are shown schematically in Figure 1(c).Table 2
Primers used to create recombinant chimeric C0C1 proteins.
cMyBP-C domainPrimer pairs (upstream/downstream primer)Mouse C05′-ATCACCATCACCATATGCCGGAGCCAGGGAAGAAACCA -3′/5′-AGGGGCCAGCATGGGTTCTGCCTTCTCTGGAGGGGC -3′Mouse P/A5′-TCTGAAGTTGCTCCAGGAGCC -3′/5′-GTCATCAGGGGCTCCCTGATG -3′Mouse C15′-GGAGCCCCCGATGACCCTATTGGCCTCTTTCTGATGCGA -3′/5′-TCAGCTAATTAAGCTTTCATCCAGAACCAATCGCCTCATGGAC -3′Human C05′-ATCACCATCACCATATGCCTGAGCCGGGGAAGAAGC - 3′/5′-TGGAGCAACTTCAGACTCTGCCTTCTCTGCCTCTATGAC -3′Human P/A5′-CCCATGCTGGCCCCTGC -3′/5′-GTCATCGGGGGCTCCAGG -3′Human C15′-GGAGCCCCTGATGACCCCATTGGCCTCTTCGTGATGC -3′/5′-TCAGCTAATTAAGCTTTCATCCGGTGCCCATGGCCT -3′


## 2.3. Native Protein Purification

Heavy meromyosin (HMM) and S1 were prepared from rabbit psoas skeletal myosin viaα-chymotryptic digest as described [13]. Bovine cardiac F-actin, tropomyosin, and troponin were purified from ether powder as described    [14–16]. Thin filaments, comprised of F-actin and regulatory proteins, were reconstituted in AB buffer (in mmol/L: 25 KCl, 25 imidazole (pH 7.4), 4 MgCl2, 1 EGTA, and 1 DTT) by combining at an actin:tropomyosin:troponin ratio of 4 : 1 : 1 for in vitro motility assays and at 7 : 2 : 2 for ATPase assays. The thin filament mix was labeled with rhodamine-phalloidin according to Homsher et al. [17] and used in in vitro motility assays.


## 2.4. Mechanical Force Measurements

Treatment of all animals was in strict accordance with guidelines and protocols established by the University of California Institutional Animal Care and Use Committee. Male Sprague-Dawley rats (200–250 g) were euthanized by intraperitoneal injection of sodium pentobarbital. Hearts were then rapidly excised and right ventricles were dissected in a Ringer’s solution at pH 7.4 (in mmol/L: 100 NaCl, 24 NaHCO3, 2.5 KCl, 1 MgSO4, 1 Na2HPO4, and 1 CaCl2). Trabeculae were permeabilized in situ by incubation of splayed ventricles overnight in a relaxing solution containing 50% glycerol and 1% Triton X-100 (Sigma, St. Louis, Missouri) at 4°C. Individual trabeculae were dissected free from ventricle walls, pinned to the bottom of a sylgar-coated Petri dish, and stored for up to one week in glycerinated relaxing solution at −20°C.Steady state force and rate of tension redevelopment (ktr) measurements were performed as previously described [6]. Briefly, permeabilized trabeculae were mounted between a force transducer (model 403A, Aurora Scientific Inc.) and a torque motor (Model 312-Cl, Aurora Scientific Inc). Sarcomere length was adjusted to ~2.3 μm in relaxing solution and monitored throughout the course of the experiment using an inverted microscope (Olympus IX-71) fitted with a 12-Mega pixel digital camera (Olympus DP70). Relaxing, preactivating, and Ca2+-activating solutions were prepared as previously described using a custom software package    [18, 19]. Solutions were maintained at 0.18 M ionic strength and pH 7.0 at 15°C (in mmol/L: 15 phosphocreatine, 15 EGTA, at least 40 MOPS, 1 free Mg2+, 135 Na+ + K+, 1 DTT, 250 units ml-1 creatine kinase (CK), and 5 ATP). Ca2+ concentration (reported as pCa = −log[Ca2+]) was established by varying amounts of CaCl2. Recombinant proteins were added to relaxing and preactivating solutions by buffer exchange using desalting spin columns (Pierce, Rockford, IL).


## 2.5. In Vitro Motility Assays

In vitro motility assays were performed as described previously [5]. Briefly, HMM was applied to a nitrocellulose-coated coverslip followed by incubation with bovine serum albumin (BSA) to prevent nonspecific adsorption of thin filaments or recombinant proteins to the motility surface. Shredded actin filaments followed by ATP were then added to block nonfunctional myosin heads (dead-heads). Next, rhodamine-phalloidin labeled bovine cardiac thin filaments (4 : 1 : 1 actin : tropomyosin : troponin) were added, followed by a motility buffer containing AB buffer, 2 mM ATP, and an oxygen-scavenging system to limit photo-bleaching. Recombinant cMyBP-C proteins were dialyzed into AB buffer and added to the slide surface in the motility buffer. Motility was viewed using an Olympus IX-71 microscope with an Hg-arc lamp, TRITC filter, and a 100×/1.4 NA oil-immersion objective. Video files were recorded using a Q-Imaging Retiga Exi digital camera and ImageProPlus software. Filament motility was analyzed using custom software developed in LabView and NI Vision Development (National Instruments, Austin, TX) kindly provided by Dr. Michael Regnier (University of Washington).


## 2.6. ATPase Assay

ATPase assays were performed by mixing 0.2μM myosin S1 and 3.5 μM reconstituted bovine cardiac thin filaments (mixed at a 7 : 2 : 2 actin:tropomyosin:troponin ratio) in ATPase buffer (in mmol/L: 10 imidazole (pH 7.4), 2 MgCl2, 1 EGTA, and 1 DTT) with or without 1 μM recombinant cMyBP-C proteins. The addition of 1 mM ATP started the reaction and reactions were quenched at three different time points with stop solution (3.3% SDS, 0.12 M Na-EDTA, pH 7.4). Phosphate production was determined via colorimetric assay as described [20].


## 2.7. Statistical Tests

Data were compared using ANOVA followed by Bonferroni post-hoc comparisons. Significance was considered atP<.05.


## 3. Results

### 3.1. Human C0C1 Increases Ca2+ Sensitivity of Tension in Permeabilized Trabeculae

Effects of recombinant mouse and human C0C1 proteins (inclusive of the C0, P/A, and C1 domains) were first assessed in permeabilized rat cardiac trabeculae. Figure2(a) shows effects of human (h)C0C1 and mouse (m)C0C1 on force generation at pCa 9.0 and at maximal Ca2+ activation (pCa 4.5). Neither hC0C1 nor mC0C1 affected resting force in the absence of Ca2+ (pCa 9.0) or maximal force at pCa 4.5 even at concentrations up to 80 μM, the highest concentration used by Herron et al. [7]. This result is consistent with observations by Razumova et al. [6] who found that preincubation of rat trabeculae with 30 μM mC0C1 did not affect force, but it differs somewhat from Herron et al. [7] who found that 30 μM hC0C1 activated force generation even in the absence of Ca2+ at pCa 9.0. Because measurements by Herron et al. [7] were conducted primarily using permeabilized myocytes from human myocardium, species-specific differences (rat versus human) could potentially contribute to the different effects observed here. However, as shown in Figures 2(b) and 2(c), hC0C1 was effective at increasing force and ktr at submaximal Ca2+ (pCa 5.3) near the half-maximal [Ca2+] (pCa50) required for maximal force generation [6]. Effects were significant after preincubation with 50 μM hC0C1. 50 μM mC0C1 also increased Ca2+ sensitivity of tension, albeit to a lesser extent than hC0C1, but had no effect on ktr.Effects of mC0C1 and hC0C1 on steady state force and rate of tension redevelopment (ktr) in permeabilized rat trabeculae. (a) Preincubation of trabeculae with 80 μM mC0C1 or hC0C1 did not affect resting force at pCa 9.0 or maximal Ca2+ activated force at pCa 4.5. However, hC0C1 increased force (b) and ktr (c) at intermediate [Ca2+] (pCa 5.3) with effects significant at 50 μM. mC0C1 increased force at 50 μM but did not significantly increase ktr. Data are mean ± SEM (n = 4 for hC0C1 and n = 3 for mC0C1). Asterisks denote significant differences in compared values obtained prior to protein addition (P<.05).
(a)(b)(c)


### 3.2. The Human Pro-Ala Rich Region Activates Filament Motility in the Absence of Ca2+

Because results from permeabilized rat trabeculae suggested that species-specific differences contribute to differences in effects of hC0C1 and mC0C1, we sought to compare effects of hC0C1 versus mC0C1 in a defined system that minimizes variability in cross-species isoform expression. We therefore compared effects of hC0C1 and mC0C1 using in vitro motility assays with reconstituted cardiac thin filaments and skeletal HMM. Control values for average filament velocities in the absence (pCa 9) and presence (pCa 5) of Ca2+ were 0.3 ± 0.1 μm/s (n = 12) and 3.8 ± 0.5 μm/s (n = 9), respectively, demonstrating that reconstituted thin filaments were well regulated by Ca2+ under control conditions in the absence of added recombinant proteins. Figure 3(a) compares filament sliding speeds at pCa 9.0 in the absence or presence of hC0C1 or mC0C1. Under control conditions at low Ca2+, filament sliding speed was low and the vast majority of filaments were stopped (fraction moving was 4%). Addition of 1 μM mC0C1 to motility buffers did not activate motility, whereas addition of hC0C1 significantly increased motility. These results are similar to those obtained in rat trabeculae (Figure 2) where hC0C1 increased Ca2+ sensitivity of tension and ktr to a greater extent than mC0C1. They are also consistent with Herron et al. [7]  who found that hC0C1 could activate tension development in myocytes in the absence of Ca2+.Effects of mouse and human C0, P/A, and C1 domains on motility of regulated thin filaments (F-actin + Tm + Tn) at pCa 9. (a) Compared to control experiments in the absence of added protein (n = 12), 1 μM hC0C1 significantly activated filament sliding speed motility and the fraction of filaments moving (n = 8), whereas 1 μM mC0C1 did not affect motility (n = 9). (b) Substitution of the human for mouse P/A domain in the mouse C0C1 backbone (mhmC0C1) activated motility (n = 7), whereas substitution of the mouse P/A domain into the human backbone (hmhC0C1) depressed motility relative to hC0C1 (n = 5). (c) Exchange of mouse and human C1 domains. The human C1 domain (mmhC0C1) activated motility (compared to mC0C1, n = 5), whereas the mouse C1 domain (hhmC0C1) depressed motility (compared to hC0C1, n = 6). (d) Exchange of mouse and human C0 domains. The human C0 (hmmC0C1, n = 5) and mouse C0 (mhhC0C1, n = 10) domains did not affect thin filament motility (compared to mC0C1 and hC0C1 controls, resp.). Data are mean ± SD. Asterisks (*) and crosses (†) denote significant differences compared to mC0C1 and hC0C1, respectively, (P<.05).
(a)(b)(c)(d)To determine whether the P/A region contributes to the activating properties of hC0C1, chimeric C0C1 proteins were created that substituted human and mouse P/A regions. Figure3(b) shows effects on filament motility of exchanging the human and mouse P/A domains in chimeric proteins. Insertion of the human P/A region into mouse C0C1 to create the chimeric protein mhmC0C1 (mouse C0, human P/A, and mouse C1) increased filament motility at pCa 9. This result demonstrates that the human P/A region but not the mouse P/A region is sufficient to confer activating effects on C0C1 proteins. However, activation was not complete since sliding speeds were still somewhat less than in the presence of hC0C1 comprised of all human sequences. This suggests that domains outside of the P/A region must also contribute to the activating effects of hC0C1. Consistent with this idea, when the mouse P/A region was exchanged into hC0C1, that is, in hmhC0C1 (human C0, mouse P/A, and human C1), filament sliding speeds were increased relative to control but were less than in the presence of hC0C1. Collectively, these results demonstrate that the human P/A rich region is sufficient to activate motility in the absence of Ca2+, but that the C0 or C1 domains must also contribute to the ability of hC0C1 to activate filament motility.


### 3.3. Human C1, but not C0, Activates Motility in the Absence of Ca2+

To determine whether human C1 or C0 domains also contribute to the activating effects of hC0C1, we created additional chimeric proteins that substituted human and mouse C0 and C1 domains. To assess if the C1 domain is required for activating effects, 1μM mmhC0C1 or hhmC0C1 proteins were added to in vitro motility assays. As shown in Figure 3(c), adding the human C1 to mouse C0 and P/A domains, (mmhC0C1) activated thin filament motility at pCa 9, demonstrating that the human C1 domain can also confer activating effects. However, filament velocity was reduced compared to hC0C1 (Figure 3(a)), indicating that other domains (e.g., P/A; Figure 3(b)) contribute to the activating effects of hC0C1. Conversely, when the mouse C1 domain was added to human C0 and P/A domains (hhmC0C1) activation occurred but to a lesser extent than hC0C1 (Figure 3(a)). These results show that mouse C1 cannot substitute for human C1 and cannot restore full activating effects when expressed with the human C0 and P/A domains.To assess whether the human C0 domain also contributes to activating effects of hC0C1, hmmC0C1 and mhhC0C1 proteins were created and their effects in motility assays were assessed at pCa 9. As shown in Figure3(d), when the human C0 domain was added to mouse P/A and C1 domains (hmmC0C1), activation of filament motility did not occur and effects of hmmC0C1 were not different from the parent mC0C1.  Similarly, when the mouse C0 domain was expressed with the human P/A and C1 domains (mhhC0C1), filament motility was the same as in the presence of hC0C1 (Figure 3(a)). These results show that C0 does not contribute to the activating effects of hC0C1 in motility assays, and that the human P/A and C1 domains together are sufficient to account for the full activating properties of hC0C1 on actomyosin interactions in the absence of Ca2+.


### 3.4. Activating Effects Are Independent of Myosin S2

When expressed in rat neonatal cardiomyocytes, hC0C1 localized to sarcomere A-bands [7], suggesting that interactions of hC0C1 with myosin or other thick filament proteins are required for the observed activating effects. Consistent with this idea, the C0 domain was reported to bind to myosin S1    [21] and C1 can bind to myosin S2 [22]. Therefore to test whether interactions with myosin S2 are required for the activating effects of the human P/A and C1 domains, effects of chimeric C0C1 proteins were assessed in ATPase assays using myosin S1 (without S2) and regulated thin filaments. As shown in Figure 4, under control conditions in the absence of recombinant proteins, ATPase rates were low (0.2 ± 0.2 sec-1, n = 17) at low Ca2+ (pCa 10) and increased (3.3 ± 1.0 sec-1, n = 16) in the presence of maximal Ca2+ (pCa 3). These results demonstrate that the thin filaments were well regulated by Ca2+. Similar to the results obtained in the in vitro motility assays, addition of 1 μM hC0C1 activated ATPase activity at pCa 10, whereas mC0C1 did not significantly affect ATPase rates. Addition of the human P/A domain or the C1 domains to the mouse C0C1 backbone (mhmC0C1 or mmhC0C1) also activated ATPase activity in the absence of Ca2+, but not to the full extent of hC0C1. Substituting the mouse P/A and C1 domains in the human C0C1 backbone (hmhC0C1 and hhmC0C1) reduced ATPase activity when compared to hC0C1. Similar to motility assays (Figure 3(d)), exchanging the C0 domains (hmmC0C1 and mhhC0C1) had no effect on the ATPase rates when compared to mC0C1 and hC0C1 controls (Figure 4). Taken together, these results confirm conclusions from the in vitro motility assays that eitherthe human P/A or C1 domains can confer activating properties to the hC0C1 protein, but that bothdomains are required for full effects. However, because all activating effects occurred in the absence of myosin S2, results from the ATPase assays demonstrate that activation does not require interactions of hC0C1 with myosin S2.Figure 4
Effects of mouse and human C0, P/A, and C1 domains on thin filament activated myosin S1 ATPase assays at pCa 10 in the absence of myosin S2. 1μM mC0C1 had no effect on ATPase activity (n = 9), whereas 1 μM hC0C1 increased ATPase activity (n = 9). The human P/A and C1 domains each activated ATPase activity (mhmC0C1 (n = 7) and mmhC0C1 (n = 5), resp.) to intermediate values. The mouse P/A and C1 domains (hmhC0C1, (n = 5) and hhmC0C1, (n = 6), resp.) depressed ATPase activity when compared to hC0C1. The human C0 domain (hmmC0C1, n = 6) had no activating effects on actin-activated S1 ATPase activity, while the mouse C0 domain (mhhC0C1, n = 4) did not affect activity when compared to hC0C1. Data are mean ± SD. Asterisks (*) and crosses (†) denote significant differences when compared to mC0C1 and hC0C1, respectively, (P<.05).


## 3.1. Human C0C1 Increases Ca2+ Sensitivity of Tension in Permeabilized Trabeculae

Effects of recombinant mouse and human C0C1 proteins (inclusive of the C0, P/A, and C1 domains) were first assessed in permeabilized rat cardiac trabeculae. Figure2(a) shows effects of human (h)C0C1 and mouse (m)C0C1 on force generation at pCa 9.0 and at maximal Ca2+ activation (pCa 4.5). Neither hC0C1 nor mC0C1 affected resting force in the absence of Ca2+ (pCa 9.0) or maximal force at pCa 4.5 even at concentrations up to 80 μM, the highest concentration used by Herron et al. [7]. This result is consistent with observations by Razumova et al. [6] who found that preincubation of rat trabeculae with 30 μM mC0C1 did not affect force, but it differs somewhat from Herron et al. [7] who found that 30 μM hC0C1 activated force generation even in the absence of Ca2+ at pCa 9.0. Because measurements by Herron et al. [7] were conducted primarily using permeabilized myocytes from human myocardium, species-specific differences (rat versus human) could potentially contribute to the different effects observed here. However, as shown in Figures 2(b) and 2(c), hC0C1 was effective at increasing force and ktr at submaximal Ca2+ (pCa 5.3) near the half-maximal [Ca2+] (pCa50) required for maximal force generation [6]. Effects were significant after preincubation with 50 μM hC0C1. 50 μM mC0C1 also increased Ca2+ sensitivity of tension, albeit to a lesser extent than hC0C1, but had no effect on ktr.Effects of mC0C1 and hC0C1 on steady state force and rate of tension redevelopment (ktr) in permeabilized rat trabeculae. (a) Preincubation of trabeculae with 80 μM mC0C1 or hC0C1 did not affect resting force at pCa 9.0 or maximal Ca2+ activated force at pCa 4.5. However, hC0C1 increased force (b) and ktr (c) at intermediate [Ca2+] (pCa 5.3) with effects significant at 50 μM. mC0C1 increased force at 50 μM but did not significantly increase ktr. Data are mean ± SEM (n = 4 for hC0C1 and n = 3 for mC0C1). Asterisks denote significant differences in compared values obtained prior to protein addition (P<.05).
(a)(b)(c)


## 3.2. The Human Pro-Ala Rich Region Activates Filament Motility in the Absence of Ca2+

Because results from permeabilized rat trabeculae suggested that species-specific differences contribute to differences in effects of hC0C1 and mC0C1, we sought to compare effects of hC0C1 versus mC0C1 in a defined system that minimizes variability in cross-species isoform expression. We therefore compared effects of hC0C1 and mC0C1 using in vitro motility assays with reconstituted cardiac thin filaments and skeletal HMM. Control values for average filament velocities in the absence (pCa 9) and presence (pCa 5) of Ca2+ were 0.3 ± 0.1 μm/s (n = 12) and 3.8 ± 0.5 μm/s (n = 9), respectively, demonstrating that reconstituted thin filaments were well regulated by Ca2+ under control conditions in the absence of added recombinant proteins. Figure 3(a) compares filament sliding speeds at pCa 9.0 in the absence or presence of hC0C1 or mC0C1. Under control conditions at low Ca2+, filament sliding speed was low and the vast majority of filaments were stopped (fraction moving was 4%). Addition of 1 μM mC0C1 to motility buffers did not activate motility, whereas addition of hC0C1 significantly increased motility. These results are similar to those obtained in rat trabeculae (Figure 2) where hC0C1 increased Ca2+ sensitivity of tension and ktr to a greater extent than mC0C1. They are also consistent with Herron et al. [7]  who found that hC0C1 could activate tension development in myocytes in the absence of Ca2+.Effects of mouse and human C0, P/A, and C1 domains on motility of regulated thin filaments (F-actin + Tm + Tn) at pCa 9. (a) Compared to control experiments in the absence of added protein (n = 12), 1 μM hC0C1 significantly activated filament sliding speed motility and the fraction of filaments moving (n = 8), whereas 1 μM mC0C1 did not affect motility (n = 9). (b) Substitution of the human for mouse P/A domain in the mouse C0C1 backbone (mhmC0C1) activated motility (n = 7), whereas substitution of the mouse P/A domain into the human backbone (hmhC0C1) depressed motility relative to hC0C1 (n = 5). (c) Exchange of mouse and human C1 domains. The human C1 domain (mmhC0C1) activated motility (compared to mC0C1, n = 5), whereas the mouse C1 domain (hhmC0C1) depressed motility (compared to hC0C1, n = 6). (d) Exchange of mouse and human C0 domains. The human C0 (hmmC0C1, n = 5) and mouse C0 (mhhC0C1, n = 10) domains did not affect thin filament motility (compared to mC0C1 and hC0C1 controls, resp.). Data are mean ± SD. Asterisks (*) and crosses (†) denote significant differences compared to mC0C1 and hC0C1, respectively, (P<.05).
(a)(b)(c)(d)To determine whether the P/A region contributes to the activating properties of hC0C1, chimeric C0C1 proteins were created that substituted human and mouse P/A regions. Figure3(b) shows effects on filament motility of exchanging the human and mouse P/A domains in chimeric proteins. Insertion of the human P/A region into mouse C0C1 to create the chimeric protein mhmC0C1 (mouse C0, human P/A, and mouse C1) increased filament motility at pCa 9. This result demonstrates that the human P/A region but not the mouse P/A region is sufficient to confer activating effects on C0C1 proteins. However, activation was not complete since sliding speeds were still somewhat less than in the presence of hC0C1 comprised of all human sequences. This suggests that domains outside of the P/A region must also contribute to the activating effects of hC0C1. Consistent with this idea, when the mouse P/A region was exchanged into hC0C1, that is, in hmhC0C1 (human C0, mouse P/A, and human C1), filament sliding speeds were increased relative to control but were less than in the presence of hC0C1. Collectively, these results demonstrate that the human P/A rich region is sufficient to activate motility in the absence of Ca2+, but that the C0 or C1 domains must also contribute to the ability of hC0C1 to activate filament motility.


## 3.3. Human C1, but not C0, Activates Motility in the Absence of Ca2+

To determine whether human C1 or C0 domains also contribute to the activating effects of hC0C1, we created additional chimeric proteins that substituted human and mouse C0 and C1 domains. To assess if the C1 domain is required for activating effects, 1μM mmhC0C1 or hhmC0C1 proteins were added to in vitro motility assays. As shown in Figure 3(c), adding the human C1 to mouse C0 and P/A domains, (mmhC0C1) activated thin filament motility at pCa 9, demonstrating that the human C1 domain can also confer activating effects. However, filament velocity was reduced compared to hC0C1 (Figure 3(a)), indicating that other domains (e.g., P/A; Figure 3(b)) contribute to the activating effects of hC0C1. Conversely, when the mouse C1 domain was added to human C0 and P/A domains (hhmC0C1) activation occurred but to a lesser extent than hC0C1 (Figure 3(a)). These results show that mouse C1 cannot substitute for human C1 and cannot restore full activating effects when expressed with the human C0 and P/A domains.To assess whether the human C0 domain also contributes to activating effects of hC0C1, hmmC0C1 and mhhC0C1 proteins were created and their effects in motility assays were assessed at pCa 9. As shown in Figure3(d), when the human C0 domain was added to mouse P/A and C1 domains (hmmC0C1), activation of filament motility did not occur and effects of hmmC0C1 were not different from the parent mC0C1.  Similarly, when the mouse C0 domain was expressed with the human P/A and C1 domains (mhhC0C1), filament motility was the same as in the presence of hC0C1 (Figure 3(a)). These results show that C0 does not contribute to the activating effects of hC0C1 in motility assays, and that the human P/A and C1 domains together are sufficient to account for the full activating properties of hC0C1 on actomyosin interactions in the absence of Ca2+.


## 3.4. Activating Effects Are Independent of Myosin S2

When expressed in rat neonatal cardiomyocytes, hC0C1 localized to sarcomere A-bands [7], suggesting that interactions of hC0C1 with myosin or other thick filament proteins are required for the observed activating effects. Consistent with this idea, the C0 domain was reported to bind to myosin S1    [21] and C1 can bind to myosin S2 [22]. Therefore to test whether interactions with myosin S2 are required for the activating effects of the human P/A and C1 domains, effects of chimeric C0C1 proteins were assessed in ATPase assays using myosin S1 (without S2) and regulated thin filaments. As shown in Figure 4, under control conditions in the absence of recombinant proteins, ATPase rates were low (0.2 ± 0.2 sec-1, n = 17) at low Ca2+ (pCa 10) and increased (3.3 ± 1.0 sec-1, n = 16) in the presence of maximal Ca2+ (pCa 3). These results demonstrate that the thin filaments were well regulated by Ca2+. Similar to the results obtained in the in vitro motility assays, addition of 1 μM hC0C1 activated ATPase activity at pCa 10, whereas mC0C1 did not significantly affect ATPase rates. Addition of the human P/A domain or the C1 domains to the mouse C0C1 backbone (mhmC0C1 or mmhC0C1) also activated ATPase activity in the absence of Ca2+, but not to the full extent of hC0C1. Substituting the mouse P/A and C1 domains in the human C0C1 backbone (hmhC0C1 and hhmC0C1) reduced ATPase activity when compared to hC0C1. Similar to motility assays (Figure 3(d)), exchanging the C0 domains (hmmC0C1 and mhhC0C1) had no effect on the ATPase rates when compared to mC0C1 and hC0C1 controls (Figure 4). Taken together, these results confirm conclusions from the in vitro motility assays that eitherthe human P/A or C1 domains can confer activating properties to the hC0C1 protein, but that bothdomains are required for full effects. However, because all activating effects occurred in the absence of myosin S2, results from the ATPase assays demonstrate that activation does not require interactions of hC0C1 with myosin S2.Figure 4
Effects of mouse and human C0, P/A, and C1 domains on thin filament activated myosin S1 ATPase assays at pCa 10 in the absence of myosin S2. 1μM mC0C1 had no effect on ATPase activity (n = 9), whereas 1 μM hC0C1 increased ATPase activity (n = 9). The human P/A and C1 domains each activated ATPase activity (mhmC0C1 (n = 7) and mmhC0C1 (n = 5), resp.) to intermediate values. The mouse P/A and C1 domains (hmhC0C1, (n = 5) and hhmC0C1, (n = 6), resp.) depressed ATPase activity when compared to hC0C1. The human C0 domain (hmmC0C1, n = 6) had no activating effects on actin-activated S1 ATPase activity, while the mouse C0 domain (mhhC0C1, n = 4) did not affect activity when compared to hC0C1. Data are mean ± SD. Asterisks (*) and crosses (†) denote significant differences when compared to mC0C1 and hC0C1, respectively, (P<.05).


## 4. Discussion

The major result from this study is that species-specific differences between mouse and human cMyBP-C contribute to functional differences in the activities of recombinant cMyBP-C proteins. In particular, we found significant sequence divergence (46% identity) in a proline-alanine (P/A) rich region near the N-terminus of the molecule. The human P/A rich region but not the mouse P/A region promoted actomyosin interactions as shown by the ability of proteins containing the human P/A region to increase thin filament motility and actomyosin ATPase activity even in the absence of Ca2+ (Figure 3). Interestingly, the human C1 domain but not mouse C1 also conferred activating effects even though the sequences of the C1 domains are much more similar (90% identity). Taken together, these results suggest that even modest sequence variations in conserved domains can lead to significant functional differences between homologous cMyBP-C proteins.Results from this study partially reconcile disparate results from two previously published studies. Using human cMyBP-C recombinant proteins, Herron et al. [7] attributed activating effects to the P/A rich region, whereas Razumova et al. [6], using mouse recombinant proteins, found that the C1 and M domains together were required to activate force. In the present study we performed a side-by-side comparison of the effects of mouse and human C0C1 proteins and found in good agreement with Herron et al. [7] that the human P/A region can confer activating effects on actomyosin interactions, whereas also in good agreement with Razumova et al. [6] that the mouse P/A region does not. Thus, species-specific differences can account in part for the different conclusions reached in the two studies.The precise mechanism(s) by which the P/A and C1 domains promote actomyosin interactions is not known, but interactions with either thick filaments    [23] or actin [3] are possible. In support of the former, hC0C1 localizes to sarcomere A-bands when expressed in rat neonatal cardiomyocytes [7] and C1 can bind to myosin S2 [22]. However, the present results exclude interactions with thick filaments because myosin S2 was not required for hC0C1 to increase acto-S1 ATPase activity (Figure 4). Furthermore, while C0 was reported to bind to myosin S1    [21], C0 made little or no contribution to the activating effects of hC0C1 (Figures 3 and 4). Thus, interactions with myosin do not appear important for the activating effects of hC0C1, although interactions with other thick filament proteins (e.g., myosin light chains) cannot be excluded. Alternatively, the P/A region could interact with thin filaments to promote activation. In support of this idea Squire et al.    [24] proposed that the P/A region of human cMyBP-C binds actin based on sequence similarity to a P/A rich segment found in essential myosin light chains (MLC). Although in a previous study we found little evidence that the mouse P/A region binds to actin because mC0C1 bound weakly if at all to F-actin in cosedimentation assays [3], Kulikovskya et al. [25] reported that human C0C1 can bind actin. Side-by-side comparisons of binding affinity are needed, but these data are thus in the right direction for the Pro-Ala sequence to contribute to functional effects in human (e.g., because of greater actin binding affinity) but exert less effects in mouse because of reduced interactions with actin    [6, 7].The functional significance of the Pro-Ala rich sequence of cMyBP-C in vivo is not known. However, the Pro-Ala rich sequences have been identified in other thick filament regulatory proteins including cardiac and skeletal isoforms of myosin essential light chains (MLC) [26]. In these proteins the Pro-Ala sequences modulate cross-bridge cycling rates and shortening velocity (Vmax) by binding to actin    [27]. The proline-alanine rich regions of different MLC isoforms slow cross-bridge kinetics either by binding directly to actin [28] or by functioning as a rigid spacer arm that extends an actin binding site located near the N-terminus of the MLC out toward the thin filament [29]. In either case, interactions with actin are thought to create a drag that limits filament sliding and slows cross-bridge cycling and shortening velocity [30]. Consistent with this idea, atrial myocytes that express an atrial MLC isoform with reduced affinity for actin have nearly twice the Vmax and maximal power output than ventricular myocytes expressing an MLC that binds to actin with greater affinity [26].By analogy with MLC, it is possible that the Pro-Ala rich region of cMyBP-C performs a similar role and contributes to the ability of cMyBP-C to limit myocyte shortening velocity, cross-bridge cycling, and power output [31]. If so, then the species-specific differences described here between the Pro-Ala regions of mouse and human cMyBP-C could serve to fine-tune shortening velocity to optimize power output (the product of force and velocity) such that contractile efficiency is maximized in hearts that contract under different hemodynamic loads and at different speeds. Consistent with this idea, we found that the percentage of proline and alanine residues in the Pro-Ala region varies inversely with heart rate in mammals such that larger mammals have a greater proportion of Pro-Ala residues, Shaffer and Harris [32]. Thus, decreased Pro-Ala content of cMyBP-C in small mammals could accelerate cross-bridge cycling kinetics, whereas increased Pro-Ala content in larger mammals could slow cycling rates. Species-specific changes in cMyBP-C could thereby fine-tune larger shifts in cross-bridge cycling kinetics that occur due to differences in isoform expression of other contractile proteins such as in myosin heavy chain that shifts from fast α-MHC (high ATPase activity and cross-bridge cycling) expressed in small mammals to slow β-MHC expressed in larger mammals    [33–36]. Such systematic changes in cross-bridge cycling kinetics could also tune cardiac relaxation rates, for instance, to ensure adequate diastolic filling times even at high heart rates in small mammals.In summary, results presented here demonstrate significant species-specific differences in the ability of the P/A rich region and C1 domains of mouse versus human cMyBP-C to activate actomyosin interactions. These differences suggest that the function of cMyBP-C varies in different species and raises the intriguing possibility that cMyBP-C-fine-tunes cardiac contraction in different animals to better match contractile speed to hemodynamic load. Experiments to test these ideas are in progress.



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*Source: 789798-2010-04-07.xml*