# Butterfly Species Diversity in Protected and Unprotected Habitat of Ise Forest Reserve, Ise Ekiti, Ekiti State

Authors: Jacob Olufemi Orimaye; Olumide Odunayo Ogunyemi; Ehi Francis Okosodo; Victor Abiodun Ojo; Tejumola Olayinka Agbelusi
Journal: Advances in Ecology
 (2016)

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## Abstract

This study investigated butterfly diversity in the protected area (PA) and unprotected area (UPA) of Ise Forest reserve, Ise Ekiti, Ekiti State, using sweep net along existing trails. Butterfly species seen in the study sites were captured and released after proper identification was made. The results indicated that a total of 837 butterflies were identified in the study sites with 661 species observed in PA and 176 species in UPA. Butterfly species diversity was significantly different (p
≤
0.05) between PA and UPA. Shannon diversity index was higher in PA (3.59) than UPA (3.27) as against Menhinick’s index, higher in UPA (2.11) than in PA (1.52). Likewise, 10 families of butterflies were recorded in PA and 8 families in UPA. The family with highest species occurrence was Satyridae (17.9%) in PA and Lycaenidae in UPA with 20.1%. Butterfly families’ diversity was not significant (p
≥
0.05) between the two study sites. Ise Forest Reserve recorded approximately 6.6% of all butterflies recorded in West Africa. The findings indicated that mature secondary and regenerated forests supported high butterfly diversity and species richness, while cultivated land and grassland had a negative impact on butterfly community suggesting the negative effect of agricultural activities on the ecosystem.


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## Body

## 1. Introduction

There is a disparity in the ecological niche for different habitats and many species of plants and animals are limited in ecological capabilities yet some are constrained to one or a small set of habitat [1]. A significant reduction in butterfly diversity is becoming gradually noticeable [2] and this reduction in diversity is largely caused by anthropogenic activities in and around their habitats [3]. However, there is a lot of anxiety on the status of the earth’s biodiversity [4] and from this observation, biodiversity is being rapidly depleted resulting in a threat to the continued support that nature make available for human existence and development [5]. Several animal species could tolerate only a limited level of human disturbances in their natural environments [6]. This is of special significance to Nigerian landscapes where human impact on land use for several hundreds of years changed the great majority of natural habitats [7]. One of the group of animals with diverse species richness is insects which represent over 50% of terrestrial biodiversity. Butterflies, unlike most of other groups of insects, are popular, well-documented, and easy to recognize. They are well adapted to the landscape and react quickly to any alteration in their habitat as a result of human-induced activities such as farmland intensification [8] and intensive logging. A viable butterfly population depends on a connection of breeding habitats scattered over the landscape which makes butterflies particularly susceptible to habitat fragmentation [9]. Moreover, the phenomenon of climate change affects quite a lot of butterflies and this has been used as a model to predict the impact of climate change on wildlife. This, however, has made butterflies one of the best species groups for monitoring biodiversity changes [9]. To this effect, Brown [10] identified that plant plays an important role in butterfly biodiversity, emphasizing that the value of the diversity of any ecosystem is viewed from the perspective of the number of species intermingling among themselves and with their physical environment. This paper, therefore, seeks to explore the differences in butterfly species composition in the protected and unprotected habitat of Ise Forest Reserve, Ekiti State.


## 2. Methodology

The study was carried out in Ise Forest Reserve, Ekiti State, Nigeria. Ise Forest Reserve covers 56.77 km2, with latitude 7.38 (7°22 60 N) and a longitude of 5.37 (5°22 0 E). The reserve can be accessed from Ise Ekiti town which is about 6 km in a straight direction to the northern edge of the reserve (Figure 1).Figure 1
Map of the study areas.


### 2.1. Data Collection

Three transects of 1 km (which has 20 sections of 50 m each) were selected from the existing trails in the protected area (PA) and unprotected area (UPA) of Ise Forest Reserve. The transect in each site was sampled 14 times for butterfly early in the morning when butterflies were active. Butterfly species seen within the range of 2.5 m to each side and 5 m in front and above were trapped using an improvised sweep net and released after proper identification [9]. All the captured butterflies that were not identified in the field were put in specimen bottles containing ethyl acetate soaked in cotton wool kept in the bottom of the jar and were later identified using the field guide to butterflies of West Africa [11] for both protected and unprotected habitats. The ecological composition of butterflies in the study areas was compared with the available knowledge of 1,000 species of butterflies recorded by Akwashiki et al. [12] for West Africa using the method of Nwosu and Iwu (Figure 2) [13].Figure 2
Comparison between the ecological composition of butterflies and the current composition of butterflies of West Africa in the study areas.


### 2.2. Data Analysis

The computer model PAST, version 3™, was used to compute diversity indices such as Shannon-Weiner index and the Simpson index of dominance in order to compare the diversities of butterflies of the two study areas. Ecological composition of butterflies in the study area was calculated using the following formula:y
/
1000
s
p
e
c
i
e
s
100
/
1%, where y is the total number of species of butterflies identified in the study areas. Student’s t-test was used to compare the differences in the mean of data collected from the two study sites and descriptive statistics such as frequency tables, charts, and percentages were used to describe results.


## 2.1. Data Collection

Three transects of 1 km (which has 20 sections of 50 m each) were selected from the existing trails in the protected area (PA) and unprotected area (UPA) of Ise Forest Reserve. The transect in each site was sampled 14 times for butterfly early in the morning when butterflies were active. Butterfly species seen within the range of 2.5 m to each side and 5 m in front and above were trapped using an improvised sweep net and released after proper identification [9]. All the captured butterflies that were not identified in the field were put in specimen bottles containing ethyl acetate soaked in cotton wool kept in the bottom of the jar and were later identified using the field guide to butterflies of West Africa [11] for both protected and unprotected habitats. The ecological composition of butterflies in the study areas was compared with the available knowledge of 1,000 species of butterflies recorded by Akwashiki et al. [12] for West Africa using the method of Nwosu and Iwu (Figure 2) [13].Figure 2
Comparison between the ecological composition of butterflies and the current composition of butterflies of West Africa in the study areas.


## 2.2. Data Analysis

The computer model PAST, version 3™, was used to compute diversity indices such as Shannon-Weiner index and the Simpson index of dominance in order to compare the diversities of butterflies of the two study areas. Ecological composition of butterflies in the study area was calculated using the following formula:y
/
1000
s
p
e
c
i
e
s
100
/
1%, where y is the total number of species of butterflies identified in the study areas. Student’s t-test was used to compare the differences in the mean of data collected from the two study sites and descriptive statistics such as frequency tables, charts, and percentages were used to describe results.


## 3. Results

### 3.1. Butterfly Species Diversity in the Study Areas

Data of butterfly species diversity is presented in Table1. A total of individual 837 butterflies were identified in the study areas. Thirty-nine species were observed in PA withCymothoe egesta of the family Lycaenidae having the highest abundance of 4.2%, followed byCitrinophila marginalis of the family Lycaenidae andMylothris chloris of Pieridae family which recorded the same value of 3.8% whilePseudacraea striataof the family Nymphalidae had the least abundance of 0.8% of the total butterflies observed in PA. In the unprotected area of the reserve, a total of 28 species of butterflies were recorded withEritis melaniabelonging to family Hesperidae having the highest abundance of 6.1% whileEuphura chalcis of Lycaenidae (1.0%) recorded the least occurrence value. Butterfly species diversity was significantly different (p
≤
0.05) (t
=
0.001) between PA and UPA.Table 1
Butterfly species diversity in the study areas.
SN
Protected area
Unprotected area
Family
Genus
Freq.
%
Family
Species
Freq
%
1
Acaridae
Acraea lycia
20
3.0
Acaridae
Bemadistes umbra
5
3.1
2
Benatistes alcinve
17
2.6
Acrarea jolutta
4
2
3
Danaidae
Amauris hecate
6
0.9
Danaidae
Danaus chrysippus
4
2
4
Danaus limniace
11
1.7
Hesperiidae
Gorgra aretina
9
5.1
5
Hesperiidae
Gomalia elma
22
Gorgra minima
7
4.1
6
Coeliades chalybe
17
Eritis melania
11
6.1
7
Eagris denuba
9
Anthene lachares
9
5.1
8
Xanthodisca astrape
20
3.0
Lycaenidae
Telipna acracea
9
5.1
9
Gorgyra heterochrus
9
1.4
Iolaus curisus
7
4.1
10
Kedestes protensa
23
3.5
Cacyreus audeoudi
9
5.1
11
Libytheidae
Libythea labdaca
7
1.1
Liptena flavicans
4
2
12
Harma theobene superna
9
1.4
Spindasis menelas
7
4.1
13
Limenitidinae
Cymothoe beckeri
20
3.0
Neptis nemetes
5
3.1
14
Hypolycaena nigra
14
2.1
Eurphura chalcis
2
1
15
Lycaenidae
Azanus ixis
11
1.7
Cynandra opis
4
2
16
Spalgis lemolea
23
3.5
Precis chorimene
7
4.1
17
Citrinophila marginalis
25
3.8
Precis oenone
9
5.1
18
Liptena august
17
2.6
Nymphalidae
Bebearia zonara
5
3.1
19
Micropentila dorothea
23
3.5
Papilionidae
Graphium pylades
5
3.1
20
Appias sabina
9
1.4
Graphium leonidas
9
5.1
21
Nymphalidae
Cymothoe egesta
28
4.2
Pieridae
Eurema brenda
7
4.1
22
Eurphene plautilla
23
3.5
Castopsilia florella
7
4.1
23
Bebearia zonara
17
2.6
Belenois theora
7
4.1
24
Hamaniida daedalus
6
0.9
Eurema hapale
4
2
25
Psudacraea striata
5
0.8
Eurema britta
5
3.1
26
Papilionidae
Graphium antheus
31
4.7
Leptosia hybrid
4
2
27
Pieridae
Appias sylvia
20
3.0
Satyridae
Elymnias phegea
7
4.1
28
Mylothris chloris
25
3.8
Bicyelus dorothea
4
2
29
Belenois calypso
14
2.1
176
100
30
Castopsilia florella
17
2.6
31
Leptosia medusa
23
3.5
32
Nepheronia thalassina
18
2.7
33
Satyridae
Ypthima doleta
14
2.1
34
Hallesis asochis
17
2.6
35
Bicyclus funebris
20
3.0
36
Melanitis parmeno
17
2.6
37
Elymnias bamboo
11
1.7
38
Bicycus xeneas
23
3.5
39
Bicycnus italus
20
3.0
661
100


### 3.2. Biodiversity Indices of Butterfly Species in the Study Area

The number of the different species and the number of individuals within each species of butterfly in the study areas (Shannon-Wiener diversity index) were higher in PA (3.59) than in UPA (3.27). The number of species found in the study areas (Menhinick’s index) was higher in UPA (2.11) than in PA (1.52) (Table2).Table 2
Biodiversity Indices of butterfly species in the study area.
PA
UPA
Taxa_S
39
28
Individuals
661
176
Dominance_D
0.03
0.04
Shannon_H
3.59
3.27
Menhinick
1.52
2.11


### 3.3. Diversity of Butterfly Family in the Study Areas

A total of 10 families representing 56% were identified at the PA and 8 families representing 44% of the collected butterfly were recorded in UPA of Ise Forest Reserve, Ekiti State, during the study period. Butterflies belonging to the family Satyridae were most abundant, representing 17.9% of the total butterflies identified in PA and the least abundance of 2.6% was observed for Papilionidae. Lycaenidae (35.7%), Pieridae (21.4%), and Hesperiidae (14.3%) were the most abundant species in UPA, respectively, whereas the families Libytheidae and Limenitidinae were completely absent.


## 3.1. Butterfly Species Diversity in the Study Areas

Data of butterfly species diversity is presented in Table1. A total of individual 837 butterflies were identified in the study areas. Thirty-nine species were observed in PA withCymothoe egesta of the family Lycaenidae having the highest abundance of 4.2%, followed byCitrinophila marginalis of the family Lycaenidae andMylothris chloris of Pieridae family which recorded the same value of 3.8% whilePseudacraea striataof the family Nymphalidae had the least abundance of 0.8% of the total butterflies observed in PA. In the unprotected area of the reserve, a total of 28 species of butterflies were recorded withEritis melaniabelonging to family Hesperidae having the highest abundance of 6.1% whileEuphura chalcis of Lycaenidae (1.0%) recorded the least occurrence value. Butterfly species diversity was significantly different (p
≤
0.05) (t
=
0.001) between PA and UPA.Table 1
Butterfly species diversity in the study areas.
SN
Protected area
Unprotected area
Family
Genus
Freq.
%
Family
Species
Freq
%
1
Acaridae
Acraea lycia
20
3.0
Acaridae
Bemadistes umbra
5
3.1
2
Benatistes alcinve
17
2.6
Acrarea jolutta
4
2
3
Danaidae
Amauris hecate
6
0.9
Danaidae
Danaus chrysippus
4
2
4
Danaus limniace
11
1.7
Hesperiidae
Gorgra aretina
9
5.1
5
Hesperiidae
Gomalia elma
22
Gorgra minima
7
4.1
6
Coeliades chalybe
17
Eritis melania
11
6.1
7
Eagris denuba
9
Anthene lachares
9
5.1
8
Xanthodisca astrape
20
3.0
Lycaenidae
Telipna acracea
9
5.1
9
Gorgyra heterochrus
9
1.4
Iolaus curisus
7
4.1
10
Kedestes protensa
23
3.5
Cacyreus audeoudi
9
5.1
11
Libytheidae
Libythea labdaca
7
1.1
Liptena flavicans
4
2
12
Harma theobene superna
9
1.4
Spindasis menelas
7
4.1
13
Limenitidinae
Cymothoe beckeri
20
3.0
Neptis nemetes
5
3.1
14
Hypolycaena nigra
14
2.1
Eurphura chalcis
2
1
15
Lycaenidae
Azanus ixis
11
1.7
Cynandra opis
4
2
16
Spalgis lemolea
23
3.5
Precis chorimene
7
4.1
17
Citrinophila marginalis
25
3.8
Precis oenone
9
5.1
18
Liptena august
17
2.6
Nymphalidae
Bebearia zonara
5
3.1
19
Micropentila dorothea
23
3.5
Papilionidae
Graphium pylades
5
3.1
20
Appias sabina
9
1.4
Graphium leonidas
9
5.1
21
Nymphalidae
Cymothoe egesta
28
4.2
Pieridae
Eurema brenda
7
4.1
22
Eurphene plautilla
23
3.5
Castopsilia florella
7
4.1
23
Bebearia zonara
17
2.6
Belenois theora
7
4.1
24
Hamaniida daedalus
6
0.9
Eurema hapale
4
2
25
Psudacraea striata
5
0.8
Eurema britta
5
3.1
26
Papilionidae
Graphium antheus
31
4.7
Leptosia hybrid
4
2
27
Pieridae
Appias sylvia
20
3.0
Satyridae
Elymnias phegea
7
4.1
28
Mylothris chloris
25
3.8
Bicyelus dorothea
4
2
29
Belenois calypso
14
2.1
176
100
30
Castopsilia florella
17
2.6
31
Leptosia medusa
23
3.5
32
Nepheronia thalassina
18
2.7
33
Satyridae
Ypthima doleta
14
2.1
34
Hallesis asochis
17
2.6
35
Bicyclus funebris
20
3.0
36
Melanitis parmeno
17
2.6
37
Elymnias bamboo
11
1.7
38
Bicycus xeneas
23
3.5
39
Bicycnus italus
20
3.0
661
100


## 3.2. Biodiversity Indices of Butterfly Species in the Study Area

The number of the different species and the number of individuals within each species of butterfly in the study areas (Shannon-Wiener diversity index) were higher in PA (3.59) than in UPA (3.27). The number of species found in the study areas (Menhinick’s index) was higher in UPA (2.11) than in PA (1.52) (Table2).Table 2
Biodiversity Indices of butterfly species in the study area.
PA
UPA
Taxa_S
39
28
Individuals
661
176
Dominance_D
0.03
0.04
Shannon_H
3.59
3.27
Menhinick
1.52
2.11


## 3.3. Diversity of Butterfly Family in the Study Areas

A total of 10 families representing 56% were identified at the PA and 8 families representing 44% of the collected butterfly were recorded in UPA of Ise Forest Reserve, Ekiti State, during the study period. Butterflies belonging to the family Satyridae were most abundant, representing 17.9% of the total butterflies identified in PA and the least abundance of 2.6% was observed for Papilionidae. Lycaenidae (35.7%), Pieridae (21.4%), and Hesperiidae (14.3%) were the most abundant species in UPA, respectively, whereas the families Libytheidae and Limenitidinae were completely absent.


## 4. Discussion

A total of 66 species of butterflies belonging to 10 families were identified during the study period from two sampled sites, namely, the protected and unprotected areas of Ise Forest Reserve. Butterfly species belonging to Satyridae and Lycaenidae families were the most abundant, accounting for 17.9% and 35.7% of the butterflies collected at PA and UPA of Ise Forest Reserve, Ise Ekiti, respectively. These observations corroborated with the observation of Brown [10], Akwashiki et al. [12], Nwosu and Iwu [13], and Kemabontal et al. [14] who reported these families as the largest of the Lepidoptera order. The high occurrence of these families in the two study areas has a positive effect for pollination in these areas because they are among the exceptional fruit-feeding butterfly community [15]. The abundance of Satyridae in the PA of Ise Forest Reserve also agrees with the observation of Nwosu and Iwu [13] where members of Satyridae were reported to be abundant in PA of Okwu Ogbaku forest reserve, Mbaitoli, Nigeria. Also, the study recorded a relatively high diversity of butterfly species and species richness in PA compared to UPA [15].The higher diversity, minimum dominance, and occurrence of 10 unique families of butterflies recorded in PA as against 8 recorded in UPA may be attributable to the vegetation complexity and multilayered canopy of the PA, which facilitate different sets of microclimates, making the habitat distinct for different butterfly species [16]. Diversity is enhanced by the presence of specialists that exhibit distinct habitat preferences [17, 18].Most of the African butterfly species are restricted to one or a smaller number of ecological zones and are, therefore, found in specific habitats [19]. For instance, there is a large difference in the total number of species found between the fauna of the forest (PA) and the derived savanna (UPA) separated by the guinea savanna [20]. Viejo et al. [21] documented about 2000 species of butterflies in West Africa. In 2005, Larsen [11] reported that the butterflies of West Africa (west of the Dahomey Gap) consist of approximately 1,000 species. Overall butterfly species recorded for the study area were 66 species translating to 6.6% of the 1000 species of butterfly in West Africa. This might be due to climate change effects on the vegetation which invariably affect the diversity of butterfly in the study areas.


## 5. Conclusion

The findings indicated that mature secondary and regenerated forests (PA) supported high butterfly diversity and species richness, while cultivated land and grassland (UPA) had a negative impact on butterfly community. The distribution of butterfly species showed a significant difference between the two habitats. This suggests the effect of agricultural activities on the ecosystem. The species recorded in Ise Forest Reserve approximately 6.6% of all butterflies recorded in West Africa.



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*Source: 7801930-2016-10-09.xml*