# 3D Plasmonic Ensembles of Graphene Oxide and Nobel Metal Nanoparticles with Ultrahigh SERS Activity and Sensitivity

Authors: Jing Lin; Xiansong Wang; Guangxia Shen; Daxiang Cui
Journal: Journal of Nanomaterials
 (2016)

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## Abstract

We describe a comparison study on 3D ensembles of graphene oxide (GO) and metal nanoparticles (silver nanoparticles (AgNPs), gold nanoparticles (GNPs), and gold nanorods (GNRs)) for surface-enhanced Raman scattering (SERS) application. For the first time, GNRs were successfully assembled on the surfaces of GO by means of electrostatic interactions without adding any surfactant. The SERS properties of GO/AgNPs, GO/GNPs, and GO/GNRs were compared using 2-mercaptopyridine (2-Mpy) as probing molecule. We found that GO/AgNPs and GO/GNPs substrates are not suitable for detecting 2-Mpy due to the very strongπ-π stacking interaction between the 2-Mpy molecules andsp
2 carbon structure of GO. Conversely, the GO/GNRs substrates show ultrahigh SERS activity and sensitivity of 2-Mpy with the detection limit as low as ~10
-
1
5 M, which is ~2-3 orders of magnitude higher than that of the corresponding GNRs.


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## Body

## 1. Introduction

Design and construction of novel assembly architectures with individual nanoparticles (NPs) promote the practical application of NPs into functional nano/microdevices [1–5]. Generally, assemblies exhibit new and unique collective properties that are different from those of both themselves and bulk counterparts [6]. Particularly, ensembles of noble-metal NPs (gold and silver), due to their unique plasmon resonance, have attracted increasing attention in optical waveguides [7], photothermal therapy [8, 9], surface-enhanced Raman scattering (SERS) [10, 11], and so on.SERS is a fascinating and potentially analytical technique to detect a wide variety of biochemicals at trace even down to single-molecular level [12, 13]. For individual noble-metal NPs (gold and silver), due to collective oscillation of conduction band electrons, when they are irradiated at the wavelength of their localized surface plasmon resonance (LSPR) peak, greatly enhanced electric (E) field area will produce in special surface confined locations (namely, hotspots) such as the tips of gold nanorods (GNRs) and gold nanostars [14, 15]. Raman scattering signals of biochemicals within the vicinity of hotspots are dramatically amplified with E
4 dependence, which is called SERS. However, for individual noble-metal NPs (gold and silver), the uncontrolled aggregation directly decide the numbered and uniformly distributed on the surface of topographically complex [16, 17]. Therefore, development of novel SERS substrates with a large number of hotspots is still challenging for ultrasensitive biochemical detection.Anisotropic GNRs with hotspots at their tips show better performance in Raman detection than spherical gold nanoparticles [18, 19]. Recently, assembly of GNRs has shown great potential to increase high density of hotspots, as a result of side-to-side, tip-to-tip, and side-to-tip plasmon coupling between adjacent GNRs [18, 20]. Yu group developed the self-assembly of GNRs in PVA nanofibers by electrospinning, which exhibited high SERS activity and sensitivity of Raman probe 3,3′-diethylthiatricarbocyanine iodide (DTTCI) with the trace level 10
-
7 M [18]. Mirkin group have successfully prepared tailorable GNR dimers in high yield and high monodispersity (~96% dimers) using on-wire lithography (OWL) method for SERS detection [16]. Afterwards, the GNR dimers were physically attached on the micrometer-sized, ultrathin, and flexible silica sheets [17]. Coupled with the ability to tailor the optical properties of each dimer and the unique ability of the nanosheets to conform to complex topographies while maintaining the geometry and integrity of the dimers, the novel nanosheets promise SERS application. However, those methods are complex and time-consuming and of high cost.Graphene oxide (GO) is an atomically ultrathin and flexible sheet with large specific surface area [21, 22]. The sheet structure promises to assemble metal nanoparticles on two accessible sides of GO to build new 3D ensembles [23–25]. Herein, we constructed three different 3D ensembles of metal nanoparticles (silver nanoparticles (AgNPs), gold nanoparticles (GNPs), and gold nanorods (GNRs)) on the surface of GO and conducted a comparison study of SERS application on them. The GO/AgNPs was synthesized by thein situ growth method. The GO/GNPs and GO/GNRs were prepared by the electrostatic self-assembly method. Their SERS properties were compared using 2-mercaptopyridine (2-Mpy) as probing molecule.


## 2. Experimental Details

### 2.1. Synthesis of GO

Water soluble GO was prepared by oxidizing graphite powder according to a modified Hummers’ method [26] and our previous reports [21, 27, 28]. The aqueous solution of the GO (0.5 mg/mL) was stored at room temperature for further use.


### 2.2. Synthesis of GO/AgNPs

The GO/AgNPs was synthesized by thein situ growth method [27]. The aqueous solution of AgNO3 (10 mM) was gradually added to 5 mL of an aqueous solution of GO (0.5 mg/mL), by 1 mL per time for five times, while under vigorous stirring at room temperature for 10 min. Then the mixed reaction solution was kept static under ambient conditions for aging.


### 2.3. Synthesis of GNPs and GO/GNPs

GNPs with a diameter of 20 nm were prepared by the use of citrate to reduce chloroauric acid in the aqueous phase [8, 9]. A certain of PEI solution (50 mg/mL) was slowly added to a GO solution (0.5 mg/mL) to give a final weight ratio of GO : PEI 2 : 1. The mixture was sonicated for over 30 min and stirred overnight. The excess PEI molecules were removed by centrifugation filtration through 100 kDa MWCO Amicon filters and washed away with water for several times with distilled water. Then 25 mL of GO-PEI aqueous solution was added to 1 mL of GNPs solution and incubated for 12 h. The GO/GNPs composite was collected by centrifugation and stored at room temperature for further characterization and use.


### 2.4. Synthesis of GNRs and GO/GNRs

GNRs were synthesized via a seed-mediated growth method [29, 30]. Before the self-assembly, the GNRs were purified by twice centrifugation at 9600 rpm for 15 min to remove the excess CTAB molecules during the synthesis of GNRs. The GNRs (0.5 mM) were dispersed in distilled water and stored at room temperature for further use.For the self-assembly of GO and GNRs, a series of mass ratio of GO and GNRs from 1 : 1 to 1 : 50 was tried in this study. A certain volume of GO (0.50 mg/mL) was added to 1 mL of the GNRs aqueous solution (0.5 mM) with vigorous shake. Then the mixed solution was sonicated for 1 min. After 24 h incubation, the GO/GNRs composite was collected by centrifugation and stored at room temperature for further characterization and use.


### 2.5. SERS Experiments

All the experiments were carried out at room temperature. A series of 2-mercaptopyridine (2-Mpy) aqueous solutions with different concentrations were prepared for SERS detection. 100μL of 2-Mpy was added to 200 μL of GO/GNRs in a centrifugal tube and kept for 5 h to reach adsorption equilibrium under ultrasound. The 2-Mpy adsorbed GO/GNRs were washed for several times with distilled water and dispersed in 100 μL of distilled water. The purified 2-Mpy adsorbed GO/GNRs aqueous solution was dropped onto a Si wafer. After evaporation of water, the Raman spectra of samples were measured.


### 2.6. Characterization

Transmission electron microscopy (TEM) images were taken by JEM-2010 (JEOL Ltd., Japan) operated at an accelerating voltage of 200 kV. Scanning electron microscopy (SEM) images were acquired on a field emission scanning electron microscope (FESEM, Zeiss Ultra). UV-Vis spectra were measured at 20°C using a Varian Cary 50 equipped with a 10 mm quartz cell, where the light path length was 1 cm. Zeta potential measurements were measured by NICOMP 380ZLS Zeta potential/particle sizer. The Fourier transform infrared (FTIR) spectra were recorded on a Bruker EQUINOX 55 FTIR Spectrometer. Raman spectra were recorded by using Renishaw-1000-type confocal Raman spectrometer equipped with excitation source of the air-cooled argon ion laser (Spectra-Physics Model 163-C4260). The excitation wavelength of 532 nm was used for GO/AgNPs and GO/GNPs samples. The excitation wavelength of 785 nm was used for GO/GNRs samples. The laser power of ca. 1.0 mW was used as the excitation source for the Raman experiments. Each spectrum was obtained using five accumulations, and the acquisition time in each case was 20 s. Data analysis was performed by a Renishaw v1.3 WIRE software.


## 2.1. Synthesis of GO

Water soluble GO was prepared by oxidizing graphite powder according to a modified Hummers’ method [26] and our previous reports [21, 27, 28]. The aqueous solution of the GO (0.5 mg/mL) was stored at room temperature for further use.


## 2.2. Synthesis of GO/AgNPs

The GO/AgNPs was synthesized by thein situ growth method [27]. The aqueous solution of AgNO3 (10 mM) was gradually added to 5 mL of an aqueous solution of GO (0.5 mg/mL), by 1 mL per time for five times, while under vigorous stirring at room temperature for 10 min. Then the mixed reaction solution was kept static under ambient conditions for aging.


## 2.3. Synthesis of GNPs and GO/GNPs

GNPs with a diameter of 20 nm were prepared by the use of citrate to reduce chloroauric acid in the aqueous phase [8, 9]. A certain of PEI solution (50 mg/mL) was slowly added to a GO solution (0.5 mg/mL) to give a final weight ratio of GO : PEI 2 : 1. The mixture was sonicated for over 30 min and stirred overnight. The excess PEI molecules were removed by centrifugation filtration through 100 kDa MWCO Amicon filters and washed away with water for several times with distilled water. Then 25 mL of GO-PEI aqueous solution was added to 1 mL of GNPs solution and incubated for 12 h. The GO/GNPs composite was collected by centrifugation and stored at room temperature for further characterization and use.


## 2.4. Synthesis of GNRs and GO/GNRs

GNRs were synthesized via a seed-mediated growth method [29, 30]. Before the self-assembly, the GNRs were purified by twice centrifugation at 9600 rpm for 15 min to remove the excess CTAB molecules during the synthesis of GNRs. The GNRs (0.5 mM) were dispersed in distilled water and stored at room temperature for further use.For the self-assembly of GO and GNRs, a series of mass ratio of GO and GNRs from 1 : 1 to 1 : 50 was tried in this study. A certain volume of GO (0.50 mg/mL) was added to 1 mL of the GNRs aqueous solution (0.5 mM) with vigorous shake. Then the mixed solution was sonicated for 1 min. After 24 h incubation, the GO/GNRs composite was collected by centrifugation and stored at room temperature for further characterization and use.


## 2.5. SERS Experiments

All the experiments were carried out at room temperature. A series of 2-mercaptopyridine (2-Mpy) aqueous solutions with different concentrations were prepared for SERS detection. 100μL of 2-Mpy was added to 200 μL of GO/GNRs in a centrifugal tube and kept for 5 h to reach adsorption equilibrium under ultrasound. The 2-Mpy adsorbed GO/GNRs were washed for several times with distilled water and dispersed in 100 μL of distilled water. The purified 2-Mpy adsorbed GO/GNRs aqueous solution was dropped onto a Si wafer. After evaporation of water, the Raman spectra of samples were measured.


## 2.6. Characterization

Transmission electron microscopy (TEM) images were taken by JEM-2010 (JEOL Ltd., Japan) operated at an accelerating voltage of 200 kV. Scanning electron microscopy (SEM) images were acquired on a field emission scanning electron microscope (FESEM, Zeiss Ultra). UV-Vis spectra were measured at 20°C using a Varian Cary 50 equipped with a 10 mm quartz cell, where the light path length was 1 cm. Zeta potential measurements were measured by NICOMP 380ZLS Zeta potential/particle sizer. The Fourier transform infrared (FTIR) spectra were recorded on a Bruker EQUINOX 55 FTIR Spectrometer. Raman spectra were recorded by using Renishaw-1000-type confocal Raman spectrometer equipped with excitation source of the air-cooled argon ion laser (Spectra-Physics Model 163-C4260). The excitation wavelength of 532 nm was used for GO/AgNPs and GO/GNPs samples. The excitation wavelength of 785 nm was used for GO/GNRs samples. The laser power of ca. 1.0 mW was used as the excitation source for the Raman experiments. Each spectrum was obtained using five accumulations, and the acquisition time in each case was 20 s. Data analysis was performed by a Renishaw v1.3 WIRE software.


## 3. Results and Discussion

Water soluble GO was synthesized by oxidizing graphite powder according to the modified Hummers method [21, 22, 26, 28]. Atomic force microscopy (AFM) image (Figure 1(a)) shows that the planar nature of GO sheets allows them to be in good contact with the flat mica substrate as well as the stacking of GO layers (white arrows). The thickness is about 0.8 nm in Figure 1(b), indicating the formation of the single-layered GO.Figure 1
(a) High-resolution AFM imaging of single-layered graphene oxide (GO) nanosheets absorbed on a mica substrate. White arrows indicate the stacking of GO layers. (b) The height profile of the AFM image.
(a)
(b)GO/AgNPs were synthesized by thein situ growth of silver nanoparticles on the surfaces of GO. As shown in Figures 2(a) and 2(b), AgNPs are distributed on two accessible surfaces of GO. The size of AgNPs is in the range of 20~30 nm. GO/GNPs were prepared by the assembly of negatively charged GNPs on the surfaces of positively charged polyethylenimine (PEI) modified GO. Figures 2(c) and 2(d) show GNPs with diameter of 20 nm distributed on two accessible surfaces of GO. GNRs with LSPR peak at 770 nm were prepared by the seed-mediated template-assisted protocol [29–31]. Before the self-assembly, the GNRs were purified by twice centrifugation at 9600 rpm for 15 min to remove the excess CTAB molecules during the synthesis of GNRs. Figure 3(a) shows the self-assembly mechanism of negatively charged GO and positively charged GNRs. To find the best mass ratio of GO and GNRs for self-assembly, a series of mass ratio from 1 : 1 to 1 : 50 was tried in this study. As shown in Figure 3(b), when the ratio is less than 1 : 5, a lot of aggregates were found in the solutions. When the ratio is over 1 : 7, the products display excellent water solubility and stability. No aggregate has been found in the solutions even stored several weeks later. The color of GO/GNRs solution shows a GO-concentration dependent change from deep to light. The representative TEM images shown in Figures 3(c) and 3(d) display GO/GNRs having two different sandwich structures: GO-GNRs-GO at the mass ratio of 1 : 2 and GNRs-GO-GNRs at the mass ratio of 1 : 10. The results indicated that the assembly strongly depended on the mass ratio of GO and GNRs.Figure 2
((a), (b)) TEM images of GO/AgNPs. ((c), (d)) TEM images of GO/GNPs. Black arrows indicate the GO nanosheets.
(a)
(b)
(c)
(d)Figure 3
(a) Scheme illustrating the self-assembly of graphene oxide (GO) and gold nanorods (GNRs). CTAB-capped GNRs and GO sheets display positive and negative charge, respectively. (b) Photographs of GO/GNRs aqueous solutions with different mass ratio. Blue box: a lot of aggregates in the solutions. Red box: no aggregates in the solutions. (c) TEM image of GO/GNRs at the mass ratio of 1 : 2 (Inset: GO-GNRs-GO sandwich). (d) TEM image of GO/GNRs at the mass ratio of 1 : 10 (Inset: GNRs-GO-GNRs sandwich).
(a)
(b)
(c)
(d)Since the density of hotspots on the GO sheet is dependent on the number of GNRs and their junctions, the proper mass of GNRs in GO/GNRs is crucial for SERS application. Two mass ratios (1 : 7 and 1 : 10) of GO and GNRs were carefully investigated by SEM and TEM. Figures4(a)–4(c) show the SEM images of GO/GNRs complex at mass ratio 1 : 7. GNRs are evenly distributed on the two accessible surfaces of GO via electrostatic interactions, with almost no free GNRs in the SEM observation. With the increase of the ratio from 1 : 7 to 1 : 10 (Figures 4(d) and 4(e)), several free GNRs were found out of GO. The density of GNRs on the surfaces of GO/GNRs (1 : 10) is markedly higher than that of GO/GNRs (1 : 7). As shown in Figure 4(f), TEM image of GO/GNRs (1 : 10) also shows the similar results with SEM images. For the assembly of GO and GNRs at 1 : 10, the GNRs are a little bit excess, which suggested that the surfaces of GO were totally covered by GNRs. The 3D topography of GO/GNRs (1 : 10) was also observed by AFM imaging (Figure 5). Therefore, the GO/GNRs (1 : 10) composite was chosen for later Raman detection.Figure 4
SEM images of GO/GNRs complex at different mass ratio of GO and GNRs. ((a)–(c)) 1 : 7 and ((d), (e)) 1 : 10. (f) TEM image of GO/GNRs complex at mass ratio 1 : 10.
(a)
(b)
(c)
(d)
(e)
(f)Figure 5
3D AFM image of GO/GNRs complex at mass ratio 1 : 10.To further study the interaction between GO and GNRs, UV-vis-NIR spectrophotometer was used to monitor the SPR absorption change of GNRs, and zeta potential measurement was employed to detect the surface charge of the resultant products. Figure6(a) shows the UV-vis-NIR spectra of GO/GNRs aqueous solutions with different mass ratio. Pure GNRs show both the transverse and the longitudinal plasmon resonance peaks at 512 and 770 nm, respectively. After GNRs assembled on the surfaces of GO, the SPR absorbance of GNRs shows GO-concentration dependent decrease along with the color gradually fading until being colorless finally [32]. The results were consistent with the observation in Figure 3(b). The intensity of longitudinal SPR peak decreased more quickly than that of the transverse SPR peak, which is probably due to the fact that the area at long axis direction of GNRs is much larger than that at the short axis direction as well as more positive charges on the area at long axis direction of GNRs. Therefore, the long axis of GNRs was bound preferentially to the surface of GO, depending on the large area and more positive charges.Figure 6
(a) UV-vis-NIR spectra of GO/GNRs aqueous solutions with different mass ratio. (b) Zeta potential of GO, GO/GNRs, and GNRs.
(a)
(b)As shown in Figure6(b), zeta potential of GO/GNRs shows GNRs-concentration dependent increase. GO exhibits a negative charge (
-
81.33
±
3.23
), due to the abundant presence of carboxyl and hydroxyl groups on their surface. GNRs display a positive charge (36.09
±
2.93), which is attributed to a lot of positive CTAB molecules on their surface. With the increase of GNRs concentration, the zeta potential of the GO/GNRs composites becomes more positive due to the assembly of positively charged GNRs on the surface of GO.To compare the efficiency of GO/GNRs as SERS substrate, GO/AgNPs and GO/GNPs composites were chosen as control (Figure7). GO/AgNPs were synthesized by thein situ growth of silver nanoparticles on the surfaces of GO (Figures 7(a) and 7(b)) [27]. GO/GNPs were prepared by the assembly of negatively charged gold nanoparticles (20 nm) on the surfaces of positively charged polyethylenimine (PEI) modified GO (Figures 7(c) and 7(d)). Curiously, even with 2-Mpy at 1 × 10
-
5 M, no Raman bands of 2-Mpy in the range of 900–1200 nm were observed for both GO/AgNPs and GO/GNPs substrates at the excitation wavelength of 532 nm (Figures 7(b) and 7(d)). In contrast, the Raman bands of 2-Mpy on the GO/GNRs are very strong at the excitation wavelength of 785 nm (Figures 7(e) and 7(f)). The peaks at 1002, 1055, 1087, and 1118 cm
-
1 are assigned to the vibration modes of the 2-Mpy molecule [33]. The results suggested that GO/AgNPs and GO/GNPs substrates are not suitable for detecting aromatic Raman probes due to the very strong π-π stacking interaction between the aromatic molecules and thesp
2 carbon structure of GO [34]. For GO/GNRs substrate, the presence of CTAB on GNRs increases the distance of GNRs and GO, thus avoiding the direct touch (π-π stacking) between Raman probes and GO. We next investigate the SERS sensitivity of the GO/GNRs. Under the dark field imaging (Figure 8(a)), we can easily find the location of GO/GNRs. As shown in Figure 8(b), the Raman detection limit is as low as 10
-
15 M, which is ~2-3 orders of magnitude higher than that of the corresponding GNRs (~4.5 × 10
-
12 M). The high sensitivity is ascribed to the high density of hotspots on the surface of GO/GNRs.Figure 7
TEM images of GO/AgNPs (a), GO/GNPs (c), and GO/GNRs (e). SERS spectra of 2-mercaptopyridine (2-Mpy) (1 × 10
-
5 M) at different substrates: GO/AgNPs (b), GO/GNPs (d), and GO/GNRs (f).Figure 8
(a) Dark field image of GO/GNRs (1 : 10). (b) SERS spectra of 2-mercaptopyridine (2-Mpy) with different concentration on the GO/GNRs (1 : 10): 1 × 10
-
9, 1 × 10
-
12, and 1 × 10
-
15 M.
(a)
(b)Several key points are responsible for the design and construction of GO/GNRs with ultrahigh density of hotspots. (1) The charge of GO(
-
81.33
±
3.23
) and GNRs (36.09
±
2.93) directly decides the interaction force between them. (2) The mass ratio of GO and GNRs directly determines sandwich structures: GO-GNRs-GO or GNRs-GO-GNRs. (3) The Raman excitation wavelength should be coupled with the LPSR peak of the inner GNRs.


## 4. Conclusions

In summary, we have developed a convenient and versatile self-assembly strategy of GO and GNRs by means of electrostatic interactions without adding any surfactant, which may be extended to construct other graphene oxide-based assemblies, for SERS application. The as-prepared GO/AgNPs and GO/GNPs substrates are not suitable for detecting 2-Mpy due to the very strongπ-π stacking interaction between the 2-Mpy molecules and thesp
2 carbon structure of GO. Conversely, the GO/GNRs architecture owns abundant hotspots and exhibits ultrahigh SERS activity and sensitivity of 2-Mpy with the detection limit as low as ~10
-
15 M.



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*Source: 7689357-2016-05-17.xml*