# Extraction of Flavonoids from Corn Silk and Biological ActivitiesIn Vitro

Authors: Shuangqi Tian; Yue Sun; Zhicheng Chen
Journal: Journal of Food Quality
 (2021)

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## Abstract

When harvesting corn, corn silk was discarded as waste, including the compounds isolated and identified from corn silk such as flavonoids, sterols, alkaloids, polysaccharides, organic acids, volatile oils, trace elements, and multivitamins. It not only pollutes the environment but also wastes resources. In this paper, extraction methods commonly used for extracting flavonoids from corn silk were reviewed, such as reagent method, enzymatic method, microwave, supercritical CO2 extraction, ultrasonic, and microwave-assisted ultrasonic. Flavonoids are natural antioxidants and have application value in scavenging free radicals, inhibiting bacteria, and regulating blood lipids. The in vitro biological activities of flavonoids from corn silk extracted by different extraction methods were also compared.


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## Body

## 1. Introduction

During the metabolism of the human body, substances such as reactive oxygen species and free radicals with strong oxidizing properties will be produced [1, 2]. If these substances are not removed in time, they would cause damage to biological membranes and cell functions and then cause aging, cardiovascular disease, and cancer [3]. Antioxidants could scavenge free radicals, thereby reducing their harm to the human body [4]. However, due to the potential safety hazards of synthetic antioxidants, the development of low-toxic, safe, and efficient natural antioxidants has become a research hotspot [5]. In recent years, many researchers have found that flavonoids are important secondary metabolites that were widely present in plant tissues [6–8]. Biological activities in vitro aroused great interest of many researchers. At present, the main problems on the antioxidant activity of flavonoids are the low purity of the extracts and the low content of potent flavonoids [9–11].Flavonoids commonly found in plants are an indispensable part of the human diet, especially for compounds found in corn silk, which have antioxidant properties and potential health benefits, and have attracted extensive attention [12]. Corn silk, also named as Maydis stigma (Zea mays hairs), which refers to the stigmas of the maize female flowers, is frequently used in traditional Chinese herbal medicines [13]. Corn silk is made from stigmas, the yellowish thread-like strands from the female flower of corn with various therapeutic values and no documented toxicity [14]. Corn silk contains abundant flavonoids. The total amount of flavonoids varies greatly with the variety, and the content is from less than 0.1% to 3% [15, 16]. Flavonoids show a variety of biological activities, such as antioxidant, antibacterial, antidiabetic, and antifatigue, and also have some clinical applications [16]. Microwave, ultrasonic, supercritical fluid extraction, and multiple extraction technologies were used to extract flavonoids effectively [16, 17]. As shown in Table 1, to date, several flavonoids such as maysin, apigmaysin, 3-methoxymaysine, ax-4-OHmaysin, and isoorientin-2″-O-a-L-rhamnoside have been isolated and identified from corn silk [12, 17, 18]. In order to clarify the extraction method and chemical composition of flavonoids in corn silk, this paper systematically studied the chemical components of corn silk and its biological activities in vitro.Table 1
Structures and molecular weight of flavonoids or flavone glycosides from corn silk.
Structures of flavonoids or flavone glycosidesChemical ingredientsMolecular weight2″-O-α-L-Rhamnosyl-6-C-quinovosylluteolin5702″-O-α-L-Rhamnosyl-6-C-fucosylluteolin5862″-O-α-L-Rhamnosyl-6-C-fucosyl-3′-methoxyluteolin6002″-O-α-L-Rhamnosyl-6-C-3″-deoxyglucosyl-3′-methoxyluteolin6086,4′-Dihydroxy-3′-methoxyflavone-7-O-glucosides451ax-5″-Methane-3′-methoxymaysin584ax-4″–OH–3′-Methoxymaysin5867,4′-Dihydroxy-3′-methoxyflavone-2″-O-α-L-rhamnosyl-6-C-fucoside602Isoorientin-2-2″-O-α-L-rhamnoside632Maysin576Maysin-3′-methyl ether590


## 2. Extraction Methods of Flavonoids from Corn Silk

About 29 flavonoids have been isolated from corn silk. Most of them are C-glycoside compounds and have the same parent nucleus of luteolin. The flavonoids of corn silk show strong biological activities [19]. They are usually combined with sugars in corn silk to form C-glycosides, and a small part exists in a free form [20]. Flavonoids are easily soluble in organic solvents such as ethanol, methanol, ethyl acetate, and ether. Therefore, the extraction of flavonoids usually uses the ethanol reflux extraction method [21]. As shown in Figure 1, different steps may be involved in the preparation of flavonoids of corn silk. In recent years, with the rapid development of microwave, ultrasonic, supercritical fluid extraction, and multiple extraction technologies, many researchers have used them as an auxiliary technology for flavonoid extraction in order to save time and maximize the flavonoid extraction rate [22, 23]. The advantages and disadvantages of various extraction methods are compared in Table 2.Figure 1
Different steps that may be involved in the preparation of flavonoids of corn silk.Table 2
The advantages and disadvantages of various extraction methods.
Extraction methodsAdvantagesDisadvantagesEthanol reflux extractionLess dosage, short extraction time, less impurities, complete extractionVolatile and toxicAqueous extractionSimple operation, low cost, suitable for large-scale productionMore impuritiesMicrowave-assisted extractionHigh selectivity, fast and efficient, stable qualityThe extraction time is too long and the temperature is too high, but the extraction rate decreasesUltrasonic-assisted extractionShort extraction time and high yield without heatingSmall extraction scale, not suitable for large-scale productionSupercritical fluid extractionLow extraction temperature, high extraction rate, low solvent consumption, no pollutionThe separation selectivity is reduced, it is not easy to obtain a pure substance, the investment is large, and the operating component is high


### 2.1. Ethanol Reflux Extraction

Ethanol reflux extraction is a traditional method for flavonoid extraction, which is widely used in the extraction of flavonoids. Flavonoid aglycones are generally insoluble or insoluble in water and easily soluble in organic solvents such as methanol, ethanol, ethyl acetate, and ether. Therefore, taking advantage of the low price and nontoxicity of ethanol, the method of refluxing ethanol is used to remove flavonoids from corn silk [24]. The factors affecting the extraction of corn flavonoids mainly include extraction time, extraction temperature, extraction times, material-liquid ratio, and ethanol concentration. The optimized extraction process is relatively simple, and the extraction rate of corn flavonoids is relatively stable. Although the ethanol reflux extraction method has the advantages of simple operation and better extraction effect, it is associated with a large amount of extraction liquid and a long extraction time. It is not conducive to saving energy and cost and hence not suitable for large-scale production. High extraction yield (5.32 mg·g-1) of total flavonoids was obtained under the optimal extraction conditions, which are 80°C for 3 h with corn silk over 60% ethanol ratio of 1 : 30 [25].


### 2.2. Aqueous Extraction

Aqueous extracts of corn silk showed a broad spectrum of antibacterial activity on the tested microorganisms. These derivatives could be potential alternatives to the traditional control of bacteria and fungi during food storage [26].For aqueous extraction, 50 g of air-dried powder was placed in distilled water and boiled for 6 h. At intervals of 3 h, the solvent was removed in vacuum. The supernatant was collected. After 6 h, the supernatant was concentrated to make the final volume to 100 ml. Finally, 50 g of material was extracted in 100 ml of distilled water giving a concentration of 500 mg/ml [27].


### 2.3. Microwave-Assisted Extraction

Microwaves are electromagnetic waves with a frequency between 300 MHz and 300 GHz and a wavelength between 1 mm and 1 m. During microwave extraction, polar substances in plant cells absorb microwave energy to generate a large amount of heat, which causes the intracellular temperature to rise rapidly and generate pressure [28]. The pressure breaks the cell membrane and cell wall, and the intracellular material is dissolved and released into the extract. Microwave-assisted extraction methods are either better or comparable with conventional solvent extraction methods [29, 30].The process of microwave-assisted extraction (MAE) of total flavonoids from corn silk and the hypolipidemia in animal models were studied. The influence of solvent concentration, microwave power, extraction time, and dose of solvent was investigated, and then, the orthogonal experiments were performed. Animal models of hyperlipidemia induced by a high-fat diet were established. The serum levels were, respectively, measured, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). The optimum extraction parameters were determined as follows: ethanol concentration (60%), power (600 W), extraction time (16 min), ratio of plant material to solvent (M/S) (1 : 20), and yield of extract (4.55%). Corn silk total flavonoids (CSTFs) significantly lowered the serum TC, TG, and LDL-C levels. Furthermore, the serum lipid level was decreased by CSTF in hyperlipidemic animal models in a dose-dependent manner [31].


### 2.4. Ultrasonic-Assisted Extraction

Ultrasonic wave is a kind of sound wave with a frequency higher than 20000 Hz. Ultrasonic waves form mechanical vibrations of medium particles in the material medium, which can cause the interaction of the medium [32]. Cavitation and thermal effects are the main effects of ultrasonic waves in the liquid. This is beneficial to increase the dissolution of flavonoids. The ultrasonic-assisted extraction method does not require any additional chemical reagents and does not destroy the flavonoid structure, so it is considered a good auxiliary extraction method [33]. Through a three-level, three-variable Box–Behnken design of response surface methodology (RSM) adopting yield as response, the optimal conditions were determined as follows: ultrasonic power of 500 W, extraction time of 20 min, material solvent ratio of 1 : 20, and ethanol concentration of 30%. Under the optimum conditions, the extraction yield of total flavonoids was 1.13% [34].


### 2.5. Supercritical Fluid Extraction

Supercritical fluid carbon dioxide (SF-CO2) extraction (SFE) of flavonoids from corn silk and its nitrite scavenging ability were investigated. The effects of extraction time, particle size, and cosolvent composition in terms of water content in ethanol were first optimized. Then, a Box–Behnken design combined with response surface methodology (RSM) was employed to study the effects of three independent variables (temperature, pressure, and cosolvent amount) on the extraction yield of flavonoids. A maximal extraction yield of flavonoids of approximately 4.24 mg/g of corn silk by SFE was obtained under optimal conditions (a temperature of 50.88°C, a pressure of 41.80 MPa, a cosolvent amount of 2.488 mL/g, and an extraction time of 120 min with 0.4 mm particle sizes and 20% aqueous ethanol as the cosolvent). Furthermore, the nitrite scavenging ability of the flavonoid-enriched SFE extracts was assessed using the Griess reagent. The flavonoid-enriched SFE extracts exhibited the highest scavenging ability on nitrite (88.1 ± 3.04%) at the concentration of 500 g/ml and at pH 3.0. The nitrite scavenging ability of the extracts appeared to be concentration-dependent but negatively correlated with the pH [35].


## 2.1. Ethanol Reflux Extraction

Ethanol reflux extraction is a traditional method for flavonoid extraction, which is widely used in the extraction of flavonoids. Flavonoid aglycones are generally insoluble or insoluble in water and easily soluble in organic solvents such as methanol, ethanol, ethyl acetate, and ether. Therefore, taking advantage of the low price and nontoxicity of ethanol, the method of refluxing ethanol is used to remove flavonoids from corn silk [24]. The factors affecting the extraction of corn flavonoids mainly include extraction time, extraction temperature, extraction times, material-liquid ratio, and ethanol concentration. The optimized extraction process is relatively simple, and the extraction rate of corn flavonoids is relatively stable. Although the ethanol reflux extraction method has the advantages of simple operation and better extraction effect, it is associated with a large amount of extraction liquid and a long extraction time. It is not conducive to saving energy and cost and hence not suitable for large-scale production. High extraction yield (5.32 mg·g-1) of total flavonoids was obtained under the optimal extraction conditions, which are 80°C for 3 h with corn silk over 60% ethanol ratio of 1 : 30 [25].


## 2.2. Aqueous Extraction

Aqueous extracts of corn silk showed a broad spectrum of antibacterial activity on the tested microorganisms. These derivatives could be potential alternatives to the traditional control of bacteria and fungi during food storage [26].For aqueous extraction, 50 g of air-dried powder was placed in distilled water and boiled for 6 h. At intervals of 3 h, the solvent was removed in vacuum. The supernatant was collected. After 6 h, the supernatant was concentrated to make the final volume to 100 ml. Finally, 50 g of material was extracted in 100 ml of distilled water giving a concentration of 500 mg/ml [27].


## 2.3. Microwave-Assisted Extraction

Microwaves are electromagnetic waves with a frequency between 300 MHz and 300 GHz and a wavelength between 1 mm and 1 m. During microwave extraction, polar substances in plant cells absorb microwave energy to generate a large amount of heat, which causes the intracellular temperature to rise rapidly and generate pressure [28]. The pressure breaks the cell membrane and cell wall, and the intracellular material is dissolved and released into the extract. Microwave-assisted extraction methods are either better or comparable with conventional solvent extraction methods [29, 30].The process of microwave-assisted extraction (MAE) of total flavonoids from corn silk and the hypolipidemia in animal models were studied. The influence of solvent concentration, microwave power, extraction time, and dose of solvent was investigated, and then, the orthogonal experiments were performed. Animal models of hyperlipidemia induced by a high-fat diet were established. The serum levels were, respectively, measured, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). The optimum extraction parameters were determined as follows: ethanol concentration (60%), power (600 W), extraction time (16 min), ratio of plant material to solvent (M/S) (1 : 20), and yield of extract (4.55%). Corn silk total flavonoids (CSTFs) significantly lowered the serum TC, TG, and LDL-C levels. Furthermore, the serum lipid level was decreased by CSTF in hyperlipidemic animal models in a dose-dependent manner [31].


## 2.4. Ultrasonic-Assisted Extraction

Ultrasonic wave is a kind of sound wave with a frequency higher than 20000 Hz. Ultrasonic waves form mechanical vibrations of medium particles in the material medium, which can cause the interaction of the medium [32]. Cavitation and thermal effects are the main effects of ultrasonic waves in the liquid. This is beneficial to increase the dissolution of flavonoids. The ultrasonic-assisted extraction method does not require any additional chemical reagents and does not destroy the flavonoid structure, so it is considered a good auxiliary extraction method [33]. Through a three-level, three-variable Box–Behnken design of response surface methodology (RSM) adopting yield as response, the optimal conditions were determined as follows: ultrasonic power of 500 W, extraction time of 20 min, material solvent ratio of 1 : 20, and ethanol concentration of 30%. Under the optimum conditions, the extraction yield of total flavonoids was 1.13% [34].


## 2.5. Supercritical Fluid Extraction

Supercritical fluid carbon dioxide (SF-CO2) extraction (SFE) of flavonoids from corn silk and its nitrite scavenging ability were investigated. The effects of extraction time, particle size, and cosolvent composition in terms of water content in ethanol were first optimized. Then, a Box–Behnken design combined with response surface methodology (RSM) was employed to study the effects of three independent variables (temperature, pressure, and cosolvent amount) on the extraction yield of flavonoids. A maximal extraction yield of flavonoids of approximately 4.24 mg/g of corn silk by SFE was obtained under optimal conditions (a temperature of 50.88°C, a pressure of 41.80 MPa, a cosolvent amount of 2.488 mL/g, and an extraction time of 120 min with 0.4 mm particle sizes and 20% aqueous ethanol as the cosolvent). Furthermore, the nitrite scavenging ability of the flavonoid-enriched SFE extracts was assessed using the Griess reagent. The flavonoid-enriched SFE extracts exhibited the highest scavenging ability on nitrite (88.1 ± 3.04%) at the concentration of 500 g/ml and at pH 3.0. The nitrite scavenging ability of the extracts appeared to be concentration-dependent but negatively correlated with the pH [35].


## 3. Biological Activities of Corn Silk

As shown in Table3, corn silk shows a variety of biological activities, such as antioxidant, antibacterial, antidiabetic, and antifatigue, and also has some clinical applications [36, 37].Table 3
Bioactivity and extraction method of flavonoids or flavone glycosides from corn silk.
BioactivityExtraction methodResultsProducing areaReferencesAntioxidant activityEthanol-water extractionThe percentage of DPPH radical scavenged by CS extract was 92.6 at a concentration of 1.6 mg/ml−1Iran[54]Three separate solvents (ethyl acetate, ethanol, and water)The ABTS inhibition of immature and mature silks was ranged from 13.4% to 35% and 15.56% to 44.36%, respectivelyMalaysia[16]Ultrasound-assisted extractionFRAP value of total flavonoids extracted from corn silk was 467.59μmol/LChina[34]Antibacterial activityThe petroleum ether (PECS), chloroform (CECS), and methanol (MECS) extractionsPECS, MECS, and flavonoids were active against eleven bacteria out of twelve bacteria. CECS was active only against five bacteriaMalaysia[46]Ethanol, chloroform, and methanol extractionsEthanol extract showed significant antimicrobial activity against both Gram-positive bacteria (13.17 to 9.45 mg/ml) and Gram-negative bacteria (12.36 to 8.15 mg/ml) bacteriaBangladesh[55]Aqueous extractionThe antimicrobial activity againstStaphylococcus aureus and Bacillus subtilis is 500 mg/ml and 62.5 mg/ml. The minimum inhibitory concentration to Candida albicans is 125 mg/mlChina[27]Antidiabetic activityEthanol extractionTreatment with 300 mg/kg or 500 mg/kg of corn silk flavonoids significantly reduced the body weight loss, water consumption, and especially the blood glucose (BG) concentration of diabetic miceChina[15]Antifatigue activityHot ethanol continuous extractionSwimming exercise results indicated that corn silk flavonoids had antifatigue activity of mice by inhibiting the production of blood lactic acidChina[17]Hot ethanol continuous extractionCorn silk flavonoids could elevate the exercise tolerance of mice and provide protection against oxidative stress induced by exhaustive exercise in miceChina[14]


### 3.1. Antioxidant Activity of Corn Silk

Radicals are molecules or fragments of molecules that possess an unpaired electron in their outer orbital. As a result of this molecular instability, radicals are highly reactive and can promote damaging oxidation reactions with cellular proteins, lipids, or DNA, leading to oxidative stress and impaired cellular function [38].The flavonoids in corn silk have good antioxidant effects, which are mainly manifested in reducing free radical generation and scavenging free radicals [39]. Antioxidant activity of corn silk 70% acetone-water extract was determined by ferric reducing antioxidant power (FRAP) test. The results showed that the antioxidant activity of corn silk extract had a good correlation with total polyphenol content (R2 = 0.9306) [40]. The relationships between phytochemicals and antioxidant activity in corn silk were evaluated and it was found that antioxidant activity was correlated with total phenolics and total flavonoids [41]. The influence of variety and harvest maturity on phytochemical content and antioxidant activity in corn silk was studied. The results showed that different parts of corn silk also differ in antioxidant activity, and high pigmentation generally occurred at the early maturity phase, and it was related directly to high antioxidant activity [42]. The nutritional compositions and antioxidative capacity from the silk obtained by immature corn and mature corn were studied [16]. The result showed that both ethanol extracts of immature and mature silks possessed strong free radical scavenging capacity compared to the water and ethyl acetate extract. It was reported that the antioxidant and free radical scavenging activities of extract and fractions from corn silk were evaluated by using in vitro antioxidant models. The results showed that N-butanol fraction, which demonstrated the highest total phenolic and flavonoid contents, exhibited the highest antioxidant and free radical scavenging activities rather than petroleum ether fraction, ethyl acetate fraction, and water fraction [4]. Antioxidant activity of the flavonoid-rich extract from corn silk was studied in vitro and in vivo. The results showed that the IC50 values of the extract for the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity were 50.73 and 0.23 mg/mL, respectively. Furthermore, the extract could significantly enhance total superoxide dismutase (T-SOD) activity, reduce malondialdehyde (MDA) content in the serum, increase glutathione content, and decrease protein carbonyl content in the liver tissue [43]. Antioxidant activity of crude flavonoid extract using STZ-induced diabetic mice was studied. The results showed that the MDA values were significantly reduced [15].


### 3.2. Antibacterial Activity of Corn Silk

Neucere reported that water extract from corn silk could inhibit the growth ofAspergillus. The active ingredients were showed to contain chitosan and dextran according to the analysis of electrophoresis, both of which had antibacterial activity [44]. The antibacterial activity of corn silk extracts against seven kinds of common pathogens and food spoilage bacteria was investigated. Various extracts were studied on the antibacterial properties, and the results showed that 90% ethanol extract had the best antibacterial effect. In addition, acidic and neutral environment could enhance the antibacterial effects of the extracts. The antibacterial activity of ethanol extracts from corn silk was also studied [45]. Two flavonoid glycosides (compounds I and II) were isolated from n-butanol fraction of methanol extract of corn silk. The chemical structures of the compounds were elucidated as maysin (I) and maysin-3′-methyl ether (II) by means of different analytical methods such as UV, IR, NMR, and MS (ESI) analyses and by comparison with those reported in the literature for the compounds. The antimicrobial activities of maysin (I) and maysin-3′-methyl ether (II) were studied against twelve bacteria and one yeast. The sensitivity of the compounds (2.0 mg/mL) towards bacteria was compared with that of standard gentamycin (50 µg/mL). Flavonoid glycosides showed a wider range of activity towards Gram-positive and Gram-negative bacteria. Comparatively, compound I exerted the highest antibacterial activity towards Gram-positive bacteria than compound II. In comparison with gentamycin, compound I showed significantly (p<0.05) higher activity against the tested bacteria except Enterobacter aerogenes, Salmonella paratyphi, and Proteus mirabilis where it exerted statistically (p<0.05) similar activity with gentamycin. But it showed lower activity towards Pseudomonas aeruginosa than gentamycin. Maysin-3′-methyl ether (II) showed comparatively lower activity than I; it seems that the presence of methoxyl substitution on the C-3′ position slightly decreases the sensitivity towards bacteria [46].


### 3.3. Antidiabetic Activity of Corn Silk

Antidiabetic activity of corn silk flavonoids (CSFs) was studied on STZ-induced diabetic mice. The results revealed that treatment with 300 mg/kg or 500 mg/kg of CSFs significantly increased the body weight, water consumption, and especially the blood glucose concentration of diabetic mice, which indicated their potential antidiabetic activities. Antidiabetic activity of corn silk crude polysaccharides on streptozotocin-induced hyperglycemic rats was studied, and its effects on hyperlipidemia, nerve excitability, and fatigue resistance were investigated. The results showed that the polysaccharides had a certain hypoglycemic effect on diabetic rats, and it also improved the symptoms of emaciation and polydipsia in diabetic rats, but the hypoglycemic effect was not as obvious as that of positive control metformin [47]. Antidiabetic activity of corn silk methanolic extract on glucose uptake by isolated rat hemidiaphragm was studied. The results showed that the treatment of corn silk increased the uptake of glucose by isolated rat hemidiaphragm significantly and was found to be more effective than insulin [48]. Corn silk aqueous extract was also shown to exhibit potent and moderate inhibitory potential against α-amylase and α-glucosidase in vitro, respectively [49]. Corn silk aqueous extract treatment markedly reduced hyperglycemia in alloxan-induced diabetic mice. According to the study, the mechanism of the hypoglycemic effect was through increasing insulin level and repairing the injured β-cells instead of increasing glycogen and inhibiting gluconeogenesis [50]. The hypoglycemic effect of corn silk flavonoids was studied on alloxan-induced diabetic mice, and the results showed that corn silk flavonoids could significantly reduce the level of blood glucose of diabetic mice and significantly slowed down the weight loss of diabetic mice. In addition, corn silk flavonoids had an obvious improving effect on the activity of superoxide dismutases in the diabetic mice and had a certain inhibitory effect on the production of lipid peroxide (MDA), which indicated that the hypoglycemic effect might be associated with antioxidant activity by improving the function of islet cells, especially β-cells. It was found that the combination of corn silk and binahong leaves could be more effective in repairing renal damage caused by oxidative stress [51].


### 3.4. Antifatigue Activity of Corn Silk

In order to clarify the mechanism of antifatigue activity of corn silk flavonoids, blood biochemical parameters were measured in the swimming-treated mice. The swimming exercise was known to induce blood biochemical changes [52]. Blood biochemical parameters are shown in Table 3. It was found that the blood lactate and blood urea nitrogen concentrations of treatment groups were significantly lower than those of the control group (p<0.05). The results suggest that corn silk flavonoids can inhibit the production of blood lactic acid during exercise and retard the formation of blood urea nitrogen (BUN) after exercise. It was known that the endurance capacity of the body markedly decreased if energy was exhausted. As glycogen was the important resource of energy during exercise, the increase of glycogen stored in the liver is advantageous to enhance the endurance of the exercise [53]. It was found that the hepatic glycogen concentration of treatment groups was higher than that of the control group (p<0.05), and the increasing rates were 260.71 and 281.25%, respectively. The results suggested that corn silk flavonoids could elevate hepatic glycogen concentration during exercise. However, the detailed mechanism was not clear. The possible reason is that corn silk flavonoids may increase the content of postexercise hepatic glycogen of mice by improving glycogen reserve, or by reducing the consumption of glycogen during exercise, or both. It still needs further study [17].


## 3.1. Antioxidant Activity of Corn Silk

Radicals are molecules or fragments of molecules that possess an unpaired electron in their outer orbital. As a result of this molecular instability, radicals are highly reactive and can promote damaging oxidation reactions with cellular proteins, lipids, or DNA, leading to oxidative stress and impaired cellular function [38].The flavonoids in corn silk have good antioxidant effects, which are mainly manifested in reducing free radical generation and scavenging free radicals [39]. Antioxidant activity of corn silk 70% acetone-water extract was determined by ferric reducing antioxidant power (FRAP) test. The results showed that the antioxidant activity of corn silk extract had a good correlation with total polyphenol content (R2 = 0.9306) [40]. The relationships between phytochemicals and antioxidant activity in corn silk were evaluated and it was found that antioxidant activity was correlated with total phenolics and total flavonoids [41]. The influence of variety and harvest maturity on phytochemical content and antioxidant activity in corn silk was studied. The results showed that different parts of corn silk also differ in antioxidant activity, and high pigmentation generally occurred at the early maturity phase, and it was related directly to high antioxidant activity [42]. The nutritional compositions and antioxidative capacity from the silk obtained by immature corn and mature corn were studied [16]. The result showed that both ethanol extracts of immature and mature silks possessed strong free radical scavenging capacity compared to the water and ethyl acetate extract. It was reported that the antioxidant and free radical scavenging activities of extract and fractions from corn silk were evaluated by using in vitro antioxidant models. The results showed that N-butanol fraction, which demonstrated the highest total phenolic and flavonoid contents, exhibited the highest antioxidant and free radical scavenging activities rather than petroleum ether fraction, ethyl acetate fraction, and water fraction [4]. Antioxidant activity of the flavonoid-rich extract from corn silk was studied in vitro and in vivo. The results showed that the IC50 values of the extract for the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity were 50.73 and 0.23 mg/mL, respectively. Furthermore, the extract could significantly enhance total superoxide dismutase (T-SOD) activity, reduce malondialdehyde (MDA) content in the serum, increase glutathione content, and decrease protein carbonyl content in the liver tissue [43]. Antioxidant activity of crude flavonoid extract using STZ-induced diabetic mice was studied. The results showed that the MDA values were significantly reduced [15].


## 3.2. Antibacterial Activity of Corn Silk

Neucere reported that water extract from corn silk could inhibit the growth ofAspergillus. The active ingredients were showed to contain chitosan and dextran according to the analysis of electrophoresis, both of which had antibacterial activity [44]. The antibacterial activity of corn silk extracts against seven kinds of common pathogens and food spoilage bacteria was investigated. Various extracts were studied on the antibacterial properties, and the results showed that 90% ethanol extract had the best antibacterial effect. In addition, acidic and neutral environment could enhance the antibacterial effects of the extracts. The antibacterial activity of ethanol extracts from corn silk was also studied [45]. Two flavonoid glycosides (compounds I and II) were isolated from n-butanol fraction of methanol extract of corn silk. The chemical structures of the compounds were elucidated as maysin (I) and maysin-3′-methyl ether (II) by means of different analytical methods such as UV, IR, NMR, and MS (ESI) analyses and by comparison with those reported in the literature for the compounds. The antimicrobial activities of maysin (I) and maysin-3′-methyl ether (II) were studied against twelve bacteria and one yeast. The sensitivity of the compounds (2.0 mg/mL) towards bacteria was compared with that of standard gentamycin (50 µg/mL). Flavonoid glycosides showed a wider range of activity towards Gram-positive and Gram-negative bacteria. Comparatively, compound I exerted the highest antibacterial activity towards Gram-positive bacteria than compound II. In comparison with gentamycin, compound I showed significantly (p<0.05) higher activity against the tested bacteria except Enterobacter aerogenes, Salmonella paratyphi, and Proteus mirabilis where it exerted statistically (p<0.05) similar activity with gentamycin. But it showed lower activity towards Pseudomonas aeruginosa than gentamycin. Maysin-3′-methyl ether (II) showed comparatively lower activity than I; it seems that the presence of methoxyl substitution on the C-3′ position slightly decreases the sensitivity towards bacteria [46].


## 3.3. Antidiabetic Activity of Corn Silk

Antidiabetic activity of corn silk flavonoids (CSFs) was studied on STZ-induced diabetic mice. The results revealed that treatment with 300 mg/kg or 500 mg/kg of CSFs significantly increased the body weight, water consumption, and especially the blood glucose concentration of diabetic mice, which indicated their potential antidiabetic activities. Antidiabetic activity of corn silk crude polysaccharides on streptozotocin-induced hyperglycemic rats was studied, and its effects on hyperlipidemia, nerve excitability, and fatigue resistance were investigated. The results showed that the polysaccharides had a certain hypoglycemic effect on diabetic rats, and it also improved the symptoms of emaciation and polydipsia in diabetic rats, but the hypoglycemic effect was not as obvious as that of positive control metformin [47]. Antidiabetic activity of corn silk methanolic extract on glucose uptake by isolated rat hemidiaphragm was studied. The results showed that the treatment of corn silk increased the uptake of glucose by isolated rat hemidiaphragm significantly and was found to be more effective than insulin [48]. Corn silk aqueous extract was also shown to exhibit potent and moderate inhibitory potential against α-amylase and α-glucosidase in vitro, respectively [49]. Corn silk aqueous extract treatment markedly reduced hyperglycemia in alloxan-induced diabetic mice. According to the study, the mechanism of the hypoglycemic effect was through increasing insulin level and repairing the injured β-cells instead of increasing glycogen and inhibiting gluconeogenesis [50]. The hypoglycemic effect of corn silk flavonoids was studied on alloxan-induced diabetic mice, and the results showed that corn silk flavonoids could significantly reduce the level of blood glucose of diabetic mice and significantly slowed down the weight loss of diabetic mice. In addition, corn silk flavonoids had an obvious improving effect on the activity of superoxide dismutases in the diabetic mice and had a certain inhibitory effect on the production of lipid peroxide (MDA), which indicated that the hypoglycemic effect might be associated with antioxidant activity by improving the function of islet cells, especially β-cells. It was found that the combination of corn silk and binahong leaves could be more effective in repairing renal damage caused by oxidative stress [51].


## 3.4. Antifatigue Activity of Corn Silk

In order to clarify the mechanism of antifatigue activity of corn silk flavonoids, blood biochemical parameters were measured in the swimming-treated mice. The swimming exercise was known to induce blood biochemical changes [52]. Blood biochemical parameters are shown in Table 3. It was found that the blood lactate and blood urea nitrogen concentrations of treatment groups were significantly lower than those of the control group (p<0.05). The results suggest that corn silk flavonoids can inhibit the production of blood lactic acid during exercise and retard the formation of blood urea nitrogen (BUN) after exercise. It was known that the endurance capacity of the body markedly decreased if energy was exhausted. As glycogen was the important resource of energy during exercise, the increase of glycogen stored in the liver is advantageous to enhance the endurance of the exercise [53]. It was found that the hepatic glycogen concentration of treatment groups was higher than that of the control group (p<0.05), and the increasing rates were 260.71 and 281.25%, respectively. The results suggested that corn silk flavonoids could elevate hepatic glycogen concentration during exercise. However, the detailed mechanism was not clear. The possible reason is that corn silk flavonoids may increase the content of postexercise hepatic glycogen of mice by improving glycogen reserve, or by reducing the consumption of glycogen during exercise, or both. It still needs further study [17].


## 4. Conclusions

The biological activity process involves many physiological challenges, which are significantly affected by biologically active substances. In this paper, ethanol, microwave, ultrasonic, supercritical fluid extraction, and multiple extraction technologies were reviewed as impactful technologies for flavonoid extraction of corn silk. Lots of studies have shown the potential benefits of ingested flavonoids against some major selected risk factors. The antioxidant, antibacterial, antidiabetic, and antifatigue activities are also described, whilein vitro and in vivo studies are mainly related to the possible mechanisms of action of flavonoids, which represent a closer approach for the substantiation of the effects of these molecules in humans and allow health benefits to be claimed for foods containing them. Corn silk shows a variety of biological activities, such as antioxidant, antibacterial, antidiabetic, and antifatigue and has some clinical applications. In the future, the structure-activity relationship of corn flavonoids should be explored in depth, and the advantages of multiple sciences should be used to provide a strong scientific basis for the further development of natural and effective new drugs, as well as a basis for the full development and utilization of corn flavonoid resources.



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*Source: 7390425-2021-03-04.xml*