# Effects of Nutrients in Substrates of Different Grains on Aflatoxin B1 Production byAspergillus flavus

Authors: Jie Liu; Lvhui Sun; Niya Zhang; Jiacai Zhang; Jiao Guo; Chong Li; Shahid Ali Rajput; Desheng Qi
Journal: BioMed Research International
 (2016)

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## Abstract

The current study was to better understand the potential factors affecting aflatoxin B1 (AFB1) accumulation varies between different grains. The nutrient composition and contents of defatted substrates were determined; additionally, according to the nutrient content of the substrates, the effects of starch, soluble sugars, amino acids, and trace elements on AFB1 production and mycelial growth in Czapek-Dox medium were examined. These results verified that removal of lipids from ground substrates significantly reduced the substrate’s potential for AFB1 production byAspergillus flavus. Maltose, glucose, sucrose, arginine, glutamic acid, aspartic acid, and zinc significantly induced AFB1 production up to 1.7- to 26.6-fold. And stachyose more significantly promotedA. flavus growth than the other nutrients. Thus, this study demonstrated that, combined with the nutrients content of grains, in addition to lipids, sucrose, stachyose, glutamic acid, and zinc might play key roles in various grains that are differentially infected byA. flavus. Particularly, two new nutrients (arginine and stachyose) of the grains we found significantly stimulate AFB1 production andA. flavus growth, respectively. The results provide new concepts for antifungal methods to protect food and animal feed from AFB1 contamination.


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## Body

## 1. Introduction

Maize, wheat, soybean, and peanut are the major economic crops in most countries. These grains and their by-products (e.g., soybean meal, peanut meal, and corn germ meal) are used extensively for food and animal feed [1]. In addition, these products provide the best natural substrate for mould, which can be easily contaminated with mycotoxins under suitable conditions [2, 3]. Recently, the problem of food and animal feed contamination with mycotoxin, particularly aflatoxin contamination, has received attention worldwide [4, 5].Aflatoxins are a secondary metabolite produced byA. flavus andA. parasiticus. These toxins mainly exist in four forms in nature: aflatoxin B1 (AFB1), aflatoxin B2, aflatoxin G1, and aflatoxin G2. AFB1 was classified as a group I human carcinogen by IARC [6]. Thus, the AFB1 contamination of crops and by-products poses a serious threat to human and animal health and is associated with enormous economic losses in agricultural economy and animal husbandry [7].Most previous studies focus on controls on AFB1 production in crops; however, few studies concerned the factors for the different AFB1 contamination in various grains. Although several researches reported that there was a relationship between lipid and AFB1 production in crops [8–12], because of the complex compositions of grains, AFB1 production not only is related to the lipids but also is associated with other nutrients, such as starch, proteins, saccharides, and trace elements. Our previous study suggested that, in addition to the lipid, the nutrient composition and content of the substrates might be the key factors affecting AFB1 production byA. parasiticus [13]. Recently, some studies reported that some nutrients were related to AFB1 biosynthesis. For example, a strong relationship between maize endosperm starch and AFB1 contamination was previously hypothesized [14, 15]. A genetically modified maize crop capable of inhibiting α-amylase activity was recommended for AFB1 contamination control [16]. However, the maize whole-kernel study of Mellon et al. [12] did not support this hypothesis. On the other hand, glucose, ribose, xylose, and glycerol were considered excellent substrates for both growth and aflatoxin production byA. parasiticus [17]. Mellon et al. [12] also reported thatA. flavus utilizes saccharides as the basic carbon source for mycelial growth and AFB1 production. In addition, Payne and Hagler Jr. [18] found that proline stimulated aflatoxin production more than asparagine, but Reddy et al. [19] reported that asparagine excellently supported the production of aflatoxin. Meanwhile, trace elements can also affect AFB1 biosynthesis. Lillehoj et al. [20] reported that trace element contents were higher in AFB1-contaminated maize germ compared with noncontaminated maize germ. Furthermore, Stossel [21] found that zinc supplementation could promote AFB1 biosynthesis in soybean, and Cuero et al. [22] reported that iron, copper, and zinc induced aflatoxin production byA. flavus. These previous studies just focused on the effects of nutrients on the production of aflatoxin byA. flavus andA. parasiticus. However, few studies researched on the relationships between the various nutrients in grains and the differential AFB1 contamination of these grains.The objectives of this study were to examine differences in the nutrient composition and content of four grains (maize, wheat, soybean, and peanut) and corn isolate tissues and according to the nutrients content of grains and isolate tissues to systematically evaluate the effects of different nutrients (lipids, amino acids, starch, soluble sugars, and trace elements) onA. flavus growth and AFB1 biosynthesis byA. flavus, to identify the nutrients responsible for the different AFB1 contamination in these grains and isolate tissues. Taken together, these results provide new concepts for antifungal methods to protect food from AFB1 contamination.


## 2. Materials and Methods

### 2.1. Samples and Reagents

Four samples (corn, wheat, peanut, and soybean) were collected from raw material manufacturers in China, and two samples (corn germ and corn endosperm) were obtained by manual dissection [12]. All samples were ground to a fine, 40-mesh powder using a laboratory mill. The samples were stored at −20°C in zipper bags prior to use. A total of six soluble sugars (stachyose, raffinose, sucrose, fructose, maltose, and glucose), five amino acids (aspartic acid, glutamic acid, arginine, alanine, and glycine), and corn oil were purchased from Aladdin Industrial Corporation (Shanghai, China) and stored at 4°C.


### 2.2. Fungal Cultures and Preparation of Spore Suspensions

A. flavus isolate (NRRL-3357) was used in this study with a known AFB1 production capacity [23] and it is widely used in laboratory and field studies in B group (B1 and B2) aflatoxins and mainly product aflatoxin B1. The isolate was maintained as a glycerol stock preparation at −80°C. It was grown on potato dextrose agar (E. Merck) medium at 30°C for 7 days. Mature spores were harvested with sterile 0.05% Tween 80 saline solution [24]. Spore suspensions were diluted to approximately 2 × 107 spores/mL or 1 × 108 spores/mL. The spore population was quantified using a haemocytometer.


### 2.3. Effects of Corn Oil and Different Substrates on AFB1 Production inA. flavus

The initial crude fat of full-fat substrates was determined using the solvent extraction method [25], and ground peanut, soybean, corn, wheat, corn germ, and corn endosperm were defatted in a Soxhlet apparatus with anhydrous diethyl and stored at −20°C in zipper bags until further use.Twenty grams of each substrate (full-fat peanut, soybean, corn, wheat, corn germ, and corn endosperm; defatted peanut, soybean, corn, wheat, corn germ, and corn endosperm) was added to a series of 150 mL Erlenmeyer flasks, which were autoclaved at 121°C for 20 min and then cooled. Corn oil was added to the corresponding flask at a rate of 10 g/100 g of defatted substrates and full-fat substrates without corn oil. All treatments consisted of five replicates, and each experiment was repeated three times. Inoculated treatments received 2 mL of anA. flavus conidial suspension (2 × 107 spores/mL) and additional sterile deionized water to adjust the moisture to 25%. Initial moisture was determined using the oven-drying method [26]. All treatments were incubated at 30°C and 85% relative humidity. Samples were collected on the 15th day to determine AFB1 production.


### 2.4. Determination of the Starch, Soluble Sugar, Amino Acid, and Trace Element Contents of Samples

Starch content was determined using the enzyme hydrolysis method [27]. Soluble sugar extraction was performed according to Mellon et al. [12] with slight modifications. Briefly, soluble sugar analysis was performed by adding 0.2 g of ground corn sample to 2.0 mL of deionized water, allowing the mixture to sit for 5 min, and then vortex mixing for 1 min. This soak/mix cycle was repeated twice, and the sample was centrifuged at 2000 g for 5 min. The supernatant was removed and stored at −20°C. Prior to drying, the sample was centrifuged at 1500 g for 5 min, and the supernatant was filtered through a 0.22 μm Millex PTFE (Tianjin, China). Next, 0.5 mL of this filtrate was evaporated under nitrogen at 50°C. The soluble sugar analysis method was performed according to Mellon et al. [28]. The amino acid hydrolysis was performed according to GB/T 18246-2000 [29], and then, the solution was filtered through a 0.22 μm PTFE filter for amino acid analysis by liquid chromatography, as previously described by Gratzfeld-Huesgen [30]. The trace element content was detected using atomic absorption spectrometry [31].


### 2.5. Effects of Starch, Soluble Sugars, Amino Acids, and Trace Elements on AFB1 Production byA. flavus

The basic medium used in this study was Czapek-Dox medium (0.3% NaNO3, 0.1% K2HPO4, 0.05% MgSO4·7H2O, 0.05% KCl, 0.001% FeSO4·7H2O, and 3.0% sucrose). Next, 30 mL of basic medium was added to a series of 100 mL Erlenmeyer flasks. Five amino acids (aspartic acid, glutamic acid, arginine, alanine, and glycine) were added to the corresponding flask at rates of 0.5, 1.0, and 2.0 g/100 mL. Four trace elements—copper (Cu, CuSO4·5H2O), iron (Fe, FeSO4·7H2O), zinc (Zn, ZnSO4·7H2O), and manganese (Mn, MnSO4·H2O)—were added to the corresponding flask; the concentrations of Cu, Fe, Zn, and Mn were 5, 10, 20, and 50 mg/L; 20, 50, 100, and 200 mg/L; 20, 50, and 100 mg/L; and 10, 20, 50, and 100 mg/L, respectively. The concentrations of starch were 0.5, 1.0, 2.0, 3.0, and 5.0 mg/L, and starch was substituted for sucrose in the basic medium. The basic medium served as a control for the three experiments. For soluble sugar incubation, six soluble sugars (stachyose, raffinose, sucrose, fructose, maltose, and glucose) were substituted for sucrose in the basic medium, and the concentrations of soluble sugars were 0.5, 1.0, 3.0, and 6.0 g/100 mL. The media were adjusted to pH 6.0 using 6 M HCl or 5 M NaOH. Inoculated treatments received 100 μL of anA. flavus conidial suspension (1 × 108 spores/mL). All treatments were maintained at 30°C with shaking at 150 rpm for 5 days. AFB1 was extracted from the liquid medium. The experiment used a total of five replicates and was performed three times. The mycelia were collected by filtration and washed with distilled water three times and then dried for 48 h at 60°C to determine the total mycelia biomass [32].


### 2.6. AFB1 Extraction and Analysis

Substrate samples were extracted to determine the AFB1 production according to the method of Ma et al. [13]. Next, 15 mL fluid medium samples were extracted three times with 20 mL (10, 5, and 5 mL) of chloroform, and the extract was evaporated under nitrogen at 60°C. The residue was stored at 4°C for AFB1 detection.The residue was redissolved in 200μL of acetonitrile/water (9 : 1, v/v) and then derivatized using 700 μL of TFA (trifluoroacetic acid)/acetic acid/water (20 : 10 : 70, v/v/v), as described by Trucksess et al. [33]. Fifty microliters of derivatized solution was filtered through Millex PTFE 0.22 μm filters (Tianjin, China) and analysed using a reversed-phase HPLC/fluorescence detection system by Abdel-Hadi et al. [34] with slight modifications. Sample extracts were analysed using an Agilent 1260 series HPLC (Waldbronn, Germany) equipped with a fluorescence detector (λ
e
x
c 360 nm; λ
e
m 440 nm) and a C18 column (250 × 4.6 mm, 5 μm; Agilent). The analysis was performed using a mobile phase of water: methanol: acetonitrile (60 : 30 : 10) at a flow rate of 1 mL/min and a run time of 20 min. The temperature of the column oven was 30°C. The mean recovery percentage for AFB1 exceeded 90%. The limit of detection of the analytical method was 1 ng·g−1.


### 2.7. Statistical Analysis

All data analyses were performed by analysis of variance (ANOVA) using the SPSS statistical analysis system software (SPSS 22, Chicago, USA) and presented as the mean ± standard deviation (SD). Significant differences were identified using Duncan’s new multiple range test at theP
=
0.05 or P
=
0.01 level.


## 2.1. Samples and Reagents

Four samples (corn, wheat, peanut, and soybean) were collected from raw material manufacturers in China, and two samples (corn germ and corn endosperm) were obtained by manual dissection [12]. All samples were ground to a fine, 40-mesh powder using a laboratory mill. The samples were stored at −20°C in zipper bags prior to use. A total of six soluble sugars (stachyose, raffinose, sucrose, fructose, maltose, and glucose), five amino acids (aspartic acid, glutamic acid, arginine, alanine, and glycine), and corn oil were purchased from Aladdin Industrial Corporation (Shanghai, China) and stored at 4°C.


## 2.2. Fungal Cultures and Preparation of Spore Suspensions

A. flavus isolate (NRRL-3357) was used in this study with a known AFB1 production capacity [23] and it is widely used in laboratory and field studies in B group (B1 and B2) aflatoxins and mainly product aflatoxin B1. The isolate was maintained as a glycerol stock preparation at −80°C. It was grown on potato dextrose agar (E. Merck) medium at 30°C for 7 days. Mature spores were harvested with sterile 0.05% Tween 80 saline solution [24]. Spore suspensions were diluted to approximately 2 × 107 spores/mL or 1 × 108 spores/mL. The spore population was quantified using a haemocytometer.


## 2.3. Effects of Corn Oil and Different Substrates on AFB1 Production inA. flavus

The initial crude fat of full-fat substrates was determined using the solvent extraction method [25], and ground peanut, soybean, corn, wheat, corn germ, and corn endosperm were defatted in a Soxhlet apparatus with anhydrous diethyl and stored at −20°C in zipper bags until further use.Twenty grams of each substrate (full-fat peanut, soybean, corn, wheat, corn germ, and corn endosperm; defatted peanut, soybean, corn, wheat, corn germ, and corn endosperm) was added to a series of 150 mL Erlenmeyer flasks, which were autoclaved at 121°C for 20 min and then cooled. Corn oil was added to the corresponding flask at a rate of 10 g/100 g of defatted substrates and full-fat substrates without corn oil. All treatments consisted of five replicates, and each experiment was repeated three times. Inoculated treatments received 2 mL of anA. flavus conidial suspension (2 × 107 spores/mL) and additional sterile deionized water to adjust the moisture to 25%. Initial moisture was determined using the oven-drying method [26]. All treatments were incubated at 30°C and 85% relative humidity. Samples were collected on the 15th day to determine AFB1 production.


## 2.4. Determination of the Starch, Soluble Sugar, Amino Acid, and Trace Element Contents of Samples

Starch content was determined using the enzyme hydrolysis method [27]. Soluble sugar extraction was performed according to Mellon et al. [12] with slight modifications. Briefly, soluble sugar analysis was performed by adding 0.2 g of ground corn sample to 2.0 mL of deionized water, allowing the mixture to sit for 5 min, and then vortex mixing for 1 min. This soak/mix cycle was repeated twice, and the sample was centrifuged at 2000 g for 5 min. The supernatant was removed and stored at −20°C. Prior to drying, the sample was centrifuged at 1500 g for 5 min, and the supernatant was filtered through a 0.22 μm Millex PTFE (Tianjin, China). Next, 0.5 mL of this filtrate was evaporated under nitrogen at 50°C. The soluble sugar analysis method was performed according to Mellon et al. [28]. The amino acid hydrolysis was performed according to GB/T 18246-2000 [29], and then, the solution was filtered through a 0.22 μm PTFE filter for amino acid analysis by liquid chromatography, as previously described by Gratzfeld-Huesgen [30]. The trace element content was detected using atomic absorption spectrometry [31].


## 2.5. Effects of Starch, Soluble Sugars, Amino Acids, and Trace Elements on AFB1 Production byA. flavus

The basic medium used in this study was Czapek-Dox medium (0.3% NaNO3, 0.1% K2HPO4, 0.05% MgSO4·7H2O, 0.05% KCl, 0.001% FeSO4·7H2O, and 3.0% sucrose). Next, 30 mL of basic medium was added to a series of 100 mL Erlenmeyer flasks. Five amino acids (aspartic acid, glutamic acid, arginine, alanine, and glycine) were added to the corresponding flask at rates of 0.5, 1.0, and 2.0 g/100 mL. Four trace elements—copper (Cu, CuSO4·5H2O), iron (Fe, FeSO4·7H2O), zinc (Zn, ZnSO4·7H2O), and manganese (Mn, MnSO4·H2O)—were added to the corresponding flask; the concentrations of Cu, Fe, Zn, and Mn were 5, 10, 20, and 50 mg/L; 20, 50, 100, and 200 mg/L; 20, 50, and 100 mg/L; and 10, 20, 50, and 100 mg/L, respectively. The concentrations of starch were 0.5, 1.0, 2.0, 3.0, and 5.0 mg/L, and starch was substituted for sucrose in the basic medium. The basic medium served as a control for the three experiments. For soluble sugar incubation, six soluble sugars (stachyose, raffinose, sucrose, fructose, maltose, and glucose) were substituted for sucrose in the basic medium, and the concentrations of soluble sugars were 0.5, 1.0, 3.0, and 6.0 g/100 mL. The media were adjusted to pH 6.0 using 6 M HCl or 5 M NaOH. Inoculated treatments received 100 μL of anA. flavus conidial suspension (1 × 108 spores/mL). All treatments were maintained at 30°C with shaking at 150 rpm for 5 days. AFB1 was extracted from the liquid medium. The experiment used a total of five replicates and was performed three times. The mycelia were collected by filtration and washed with distilled water three times and then dried for 48 h at 60°C to determine the total mycelia biomass [32].


## 2.6. AFB1 Extraction and Analysis

Substrate samples were extracted to determine the AFB1 production according to the method of Ma et al. [13]. Next, 15 mL fluid medium samples were extracted three times with 20 mL (10, 5, and 5 mL) of chloroform, and the extract was evaporated under nitrogen at 60°C. The residue was stored at 4°C for AFB1 detection.The residue was redissolved in 200μL of acetonitrile/water (9 : 1, v/v) and then derivatized using 700 μL of TFA (trifluoroacetic acid)/acetic acid/water (20 : 10 : 70, v/v/v), as described by Trucksess et al. [33]. Fifty microliters of derivatized solution was filtered through Millex PTFE 0.22 μm filters (Tianjin, China) and analysed using a reversed-phase HPLC/fluorescence detection system by Abdel-Hadi et al. [34] with slight modifications. Sample extracts were analysed using an Agilent 1260 series HPLC (Waldbronn, Germany) equipped with a fluorescence detector (λ
e
x
c 360 nm; λ
e
m 440 nm) and a C18 column (250 × 4.6 mm, 5 μm; Agilent). The analysis was performed using a mobile phase of water: methanol: acetonitrile (60 : 30 : 10) at a flow rate of 1 mL/min and a run time of 20 min. The temperature of the column oven was 30°C. The mean recovery percentage for AFB1 exceeded 90%. The limit of detection of the analytical method was 1 ng·g−1.


## 2.7. Statistical Analysis

All data analyses were performed by analysis of variance (ANOVA) using the SPSS statistical analysis system software (SPSS 22, Chicago, USA) and presented as the mean ± standard deviation (SD). Significant differences were identified using Duncan’s new multiple range test at theP
=
0.05 or P
=
0.01 level.


## 3. Results

### 3.1. Effects of Oil and Different Substrates on AFB1 Production inA. flavus

Table1 showed that the six defatted substrates were slightly contaminated byA. flavus and had relatively low levels of AFB1 accumulation. However, AFB1 accumulation sharply increased with the addition of corn oil. The concentrations of AFB1 in full-fat substrates were also higher than in the defatted substrates. Specifically, when corn oil was added to defatted wheat, the AFB1 concentration (14.87 mg/kg) increased 495- (0.03 mg/kg) and 135-fold (0.11 mg/kg) compared to defatted wheat and full-fat wheat, respectively. Other groups also showed the same trend, except the corn germ and soybean group which have abundant lipids in full-fat substrates. However, different substrates have different effects on AFB1 production. Avoiding the effect of lipids, when substrates were defatted, the maximum AFB1 concentrations were found in defatted soybean (8.15 mg/kg), followed by defatted corn germ, peanut, corn endosperm, corn, and wheat.Table 1
Effects of corn oil and different substrates on AFB1 production by A. flavusNRRL3357.
AflatoxinB
1
D
Defatted substrates
Defatted substrates + corn oilE
Full-fat substrates
Soybean
8.15 ± 2.13B
52.06 ± 8.31A
66.26 ± 11.95A
Peanut
3.04 ± 0.33C
22.55 ± 7.07A
10.14 ± 1.07B
Corn
0.04 ± 0.02B
9.46 ± 0.56A
0.12 ± 0.01B
Wheat
0.03 ± 0.00B
14.87 ± 5.11A
0.11 ± 0.05B
Corn endosperm
0.41 ± 0.11B
5.49 ± 1.54A
0.17 ± 0.04B
Corn germ
6.82 ± 1.14C
33.03 ± 3.49B
48.21 ± 7.29A
A
–
CMeans within rows followed by the same letter are not significantly different at P
≥
0.05.
DMean aflatoxin B1 is expressed in milligrams per kilogram (n
=
5).
EAn average of 10% corn oil was added to defatted substrates.


### 3.2. Starch, Soluble Sugars, Amino Acids, and Trace Elements Contents of Different Substrates

The four main nutrients (starch, soluble sugars, amino acids, and trace elements) of defatted substrates were examined. The nutrients composition and content of six defatted substrates were very different. The starch contents of defatted wheat, corn, and corn endosperm were 67.22%, 72.21%, and 82.19%, respectively, and were significantly higher than those of the other three substrates (Table2).Table 2
Starch, trace element, soluble sugar, and amino acid contents of different substrates.
Nutrients
Defatted soybean
Defatted peanut
Defatted wheat
Defatted corn
Defatted corn endosperm
Defatted corn germ
Starch content (%)
Starch
5.26 ± 0.94E
7.01 ± 0.71E
67.22 ± 2.87C
72.21 ± 1.97B
82.19 ± 1.11A
28.12 ± 0.76D
Trace elements contents (mg/kg)
Copper
25.33 ± 9.41A
B
34.63 ± 2.44A
12.12 ± 1.90C
D
6.89 ± 0.35D
6.79 ± 1.70D
19.86 ± 3.21B
C
Iron
93.47 ± 0.22B
64.18 ± 7.68C
83.97 ± 8.51B
21.95 ± 4.15D
6.23 ± 3.36D
145.05 ± 11.31A
Zinc
51.90 ± 4.07C
84.40 ± 10.88B
39.14 ± 3.23D
18.96 ± 1.52E
12.47 ± 0.91E
107.47 ± 1.94A
Manganese
36.19 ± 0.65B
59.86 ± 1.92A
60.30 ± 0.15A
5.28 ± 0.18D
2.13 ± 0.22E
30.54 ± 0.27C
Soluble sugar concentrations (mg/g)
Fructose
3.89 ± 1.65B
C
11.12 ± 2.06A
1.94 ± 0.06C
7.72 ± 0.18A
B
1.88 ± 0.24C
9.67 ± 2.65A
Glucose
1.61 ± 0.52B
4.92 ± 1.12B
4.01 ± 0.13B
4.95 ± 0.12B
7.21 ± 0.07B
13.75 ± 4.27A
Sucrose
31.17 ± 5.05A
32.5 ± 0.46A
2.24 ± 0.12B
2.15 ± 0.33B
5.07 ± 0.1B
42.36 ± 9.19A
Maltose
0.63 ± 0.14C
0.54 ± 0.07C
4.10 ± 0.35B
4.05 ± 0.35B
7.96 ± 0.60A
1.66 ± 0.28C
Raffinose
3.32 ± 0.41B
2.08 ± 0.27B
3.00 ± 0.05B
1.11 ± 0.17B
1.16 ± 0.03B
10.71 ± 2.62A
Stachyose
34.71 ± 7.39A
6.78 ± 0.98B
0.36 ± 0.06B
NDB
NDB
0.72 ± 0.10B
Amino acid contents (%)
Aspartic acid
6.3 ± 0.38
7.70 ± 0.23
0.74 ± 0.04
0.54 ± 0.01
0.51 ± 0.15
1.81 ± 0.05
Glutamic acid
10.56 ± 0.57
13.89 ± 0.39
4.56 ± 0.24
1.72 ± 0.01
1.93 ± 0.20
3.33 ± 0.05
Glycine
2.46 ± 0.10
3.35 ± 0.15
0.55 ± 0.03
0.34 ± 0.00
0.30 ± 0.02
1.16 ± 0.02
Arginine
3.81 ± 0.18
6.95 ± 0.18
0.65 ± 0.04
0.43 ± 0.00
0.37 ± 0.03
1.73 ± 0.04
Alanine
2.58 ± 0.11
2.97 ± 0.06
0.51 ± 0.03
0.61 ± 0.01
0.66 ± 0.01
1.37 ± 0.01
Protein level
45.11 ± 2.53
55.45 ± 1.82
12.25 ± 0.37
8.36 ± 0.09
8.60 ± 1.20
19.47 ± 0.42
A–EMeans within rows followed by the same letter are not significantly different at P
≥
0.01 according to Duncan’s multiple range test (n
=
3).The concentrations of six soluble sugars (fructose, glucose, sucrose, maltose, raffinose, and stachyose) are shown in Table2. Soluble sugars were most abundant in defatted corn germ, followed by defatted soybean and peanut. Specifically, there were high concentrations of sucrose in defatted corn germ (42.36 mg/g), soybean (31.17 mg/g), and peanut (32.50 mg/g). Furthermore, the stachyose content was particularly high in defatted soybean (34.71 mg/g) (P
<
0.01).This study determined the contents of sixteen amino acids, and based on previous studies and differences in the amino acid contents, only five amino acids contents are shown in Table2; the others are not shown. The protein content was the sum of the contents of the sixteen amino acids analysed. Among the studied amino acids, glutamic acid had the highest content, followed by aspartic acid. Although the other three amino acid contents were different, these differences were not obvious compared with the contents of glutamic acid and aspartic acid. The amino acid contents of defatted peanuts and soybeans were relatively high compared with those of other grains and isolate tissues. Specifically, the glutamic acid contents of defatted soybean and peanut were 10.56% and 13.89%, respectively.The trace element contents of different grains were fairly different. The maximum content of Cu was 34.63 mg/kg in defatted peanut, and its minimum content (6.89 mg/kg) was observed in defatted corn. The maximum contents of Fe and Zn were found in defatted corn germ (145.05 and 107.47 mg/kg), and their minimum contents were identified in defatted corn endosperm. The defatted peanut and wheat were rich in Mn. Thus, the content of the four trace elements was the most abundant in defatted corn germ, followed by defatted soybean and peanut, whereas it was minimal in corn endosperm (Table2).


### 3.3. Effects of Starch, Soluble Sugars, Amino Acids, and Trace Elements on AFB1 Production

According to the solubility of the starch, this study selected five concentrations from 0.5% to 5.0%, and basal medium served as a control. These results showed that starch did not promote AFB1 production and inhibited AFB1 synthesis. The AFB1 content of control was 194.4 ng/30 mL, but in the five treatments, the maximum content of AFB1 was only 7.64 ng/30 mL which was found in 2.0% starch.The effect of soluble sugars on AFB1 production is shown in Table 3. The soluble sugar addition concentrations depend on the contents of the six substrates. These results indicated that low concentrations of soluble sugars could not promote AFB1 biosynthesis; however, when the soluble sugar concentration reached 3.0% and 6.0%, the soluble sugars promoted AFB1 production somewhat, including glucose, sucrose, and maltose (P
<
0.05). When the concentration was 3.0%, sucrose exhibited the highest promoting effect on AFB1 production (39782.61 ng/30 mL), followed by maltose (23687.29 ng/30 mL) and fructose, which had the smallest effect. However, when the concentration was 6.0%, the enhancing effects of sucrose (4471.97 ng/30 mL), raffinose (5.50 ng/30 mL), and stachyose (120.61 ng/30 mL) were reduced. Maltose promoted aflatoxin production to the greatest extent (74848.68 ng/30 mL), followed by glucose (35860.57 ng/30 mL). Among the six soluble sugars, raffinose exhibited the weakest enhancement.Table 3
Effect of soluble sugars on AFB1 production.
AflatoxinB
1
D
Concentration of soluble sugars (%)
0.5
1.0
3.0
6.0
Fructose
46.92 ± 11.30B
7.54 ± 0.83B
5.41 ± 0.56B
1137.56 ± 308.79A
Glucose
28.77 ± 3.33B
8.92 ± 0.77B
5664.33 ± 570.70B
35860.57 ± 7028.16A
Sucrose
4.63 ± 1.26B
4.28 ± 0.60B
39782.61 ± 5896.87A
4471.97 ± 442.68B
Maltose
2.84 ± 0.85C
2.34 ± 0.33C
23687.29 ± 5777.01B
74848.68 ± 8422.06A
Raffinose
5.70 ± 1.13B
2.76 ± 0.65B
208.27 ± 24.28A
5.50 ± 1.21B
Stachyose
17.02 ± 1.22B
10.08 ± 1.39B
5426.44 ± 292.49A
120.61 ± 16.55B
A–CMean values with different letters for each soluble sugar are significantly different (P
<
0.05).
DAflatoxin B1 values are the average of five replications with standard deviation in ng/30 mL.The amounts of amino acid added in this study were based on the concentrations used by Payne and Hagler Jr. [18] and amino acid concentrations of the substrates (Table 2). As the amount of amino acids increased, aflatoxin synthesis decreased (Figure 1). However, only 0.5% glutamic acid, aspartic acid, and glycine significantly stimulated AFB1 production (P
<
0.05), whereas all concentrations of arginine significantly stimulated AFB1 production (P
<
0.05). Moreover, 0.5 and 2.0% alanine reduced AFB1 production.Figure 1
Effects of glutamic acid (a), aspartic acid (b), arginine (c), alanine (d), and glycine (e) on AFB1 accumulation (ng/30 mL) at concentrations of 0, 0.5, 1.0, and 2.0 g/100 mL with incubation on a constant temperature shaker (30°C) for 5 days. The data represent the mean ± SD. Different superscript letters represent significant differences at P
<
0.05, and all treatments consisted of five replicates.
(a)
(b)
(c)
(d)
(e)Different trace elements were selected to obtain different gradient concentrations depending on the trace element contents of the six substrates (Table4). Only zinc significantly promoted AFB1 biosynthesis, and as the zinc concentration increased, AFB1 accumulation also increased. When the concentrations of zinc were 20, 50, and 100 mg/L, the contents of AFB1 were increased 4-, 5-, and 19-fold, respectively. In addition, the other three trace elements significantly inhibited AFB1 production (P
<
0
.
05) at all concentrations examined.Table 4
Effect of trace elements on AFB1 production.
AflatoxinB
1
D
Control
Concentration of trace elements (mg/L)
5
10
20
50
100
200
Copper
238.35 ± 36.61A
4.39 ± 0.29B
4.90 ± 0.59B
3.00 ± 0.94B
4.31 ± 0.08B
—
—
Iron
238.35 ± 36.61A
—
—
2.39 ± 0.08B
2.71 ± 1.21B
0.36 ± 0.23B
0.73 ± 0.20B
Zinc
238.35 ± 36.61C
—
—
954.95 ± 21.15B
C
1232.08 ± 312.71B
4733.88 ± 795.24A
—
Manganese
238.35 ± 36.61A
—
1.75 ± 0.14B
17.87 ± 3.53B
29.30 ± 4.87B
10.00 ± 3.18B
—
—: concentration not studied.
A–CMean values with different letters for each trace element are significantly different (P
<
0.05).
DAflatoxin B1 values are the average of five replications with standard deviation in ng/30 mL.


### 3.4. Effects of Starch, Soluble Sugars, Amino Acids, and Trace Elements onA. flavus Growth

The dry weights of the starch samples indicated that when more starch was present, mycelial growth was enhanced (data not shown). The dose relationship between the mycelial growth and soluble sugars revealed that higher concentrations of soluble sugars resulted in improved mycelial growth. The growth-promoting effect of stachyose was obvious, and 3.0 and 6.0% stachyose increased mycelial growth more than 2-fold compared to the other soluble sugars (Figure2). The effects of amino acids onA. flavus were also evaluated (Figure 3). Glutamic acid, aspartic acid, arginine, and alanine stimulated mycelial growth as their concentrations increased. Low concentrations of glycine promoted mycelial growth, but no significant effect was observed with 2.0% glycine. All studied trace elements, except copper, were able to promoteA. flavus mycelial growth to varying degrees. In contrast, copper significantly reduced mycelial growth at four different concentrations (Figure 4).Figure 2
Effects of soluble sugars onA. flavus growth. Different superscript letters represent significant differences at P
<
0.05 for each soluble sugar. Error bars show standard deviations of the results of five replications.Figure 3
Effects of amino acids onA. flavus growth. The data represent the mean ± SD. Letters indicate statistically significant differences (P
<
0.05) for each amino acid, and all treatments consisted of five replicates.Figure 4
Effects of copper (a), iron (b), zinc (c), and manganese (d) onA. flavus growth. Each treatment was grown as described in Section 2. The data represent the mean ± SD. Different superscript letters represent significant differences at P
<
0.05, and all treatments consisted of five replicates.
(a)
(b)
(c)
(d)


## 3.1. Effects of Oil and Different Substrates on AFB1 Production inA. flavus

Table1 showed that the six defatted substrates were slightly contaminated byA. flavus and had relatively low levels of AFB1 accumulation. However, AFB1 accumulation sharply increased with the addition of corn oil. The concentrations of AFB1 in full-fat substrates were also higher than in the defatted substrates. Specifically, when corn oil was added to defatted wheat, the AFB1 concentration (14.87 mg/kg) increased 495- (0.03 mg/kg) and 135-fold (0.11 mg/kg) compared to defatted wheat and full-fat wheat, respectively. Other groups also showed the same trend, except the corn germ and soybean group which have abundant lipids in full-fat substrates. However, different substrates have different effects on AFB1 production. Avoiding the effect of lipids, when substrates were defatted, the maximum AFB1 concentrations were found in defatted soybean (8.15 mg/kg), followed by defatted corn germ, peanut, corn endosperm, corn, and wheat.Table 1
Effects of corn oil and different substrates on AFB1 production by A. flavusNRRL3357.
AflatoxinB
1
D
Defatted substrates
Defatted substrates + corn oilE
Full-fat substrates
Soybean
8.15 ± 2.13B
52.06 ± 8.31A
66.26 ± 11.95A
Peanut
3.04 ± 0.33C
22.55 ± 7.07A
10.14 ± 1.07B
Corn
0.04 ± 0.02B
9.46 ± 0.56A
0.12 ± 0.01B
Wheat
0.03 ± 0.00B
14.87 ± 5.11A
0.11 ± 0.05B
Corn endosperm
0.41 ± 0.11B
5.49 ± 1.54A
0.17 ± 0.04B
Corn germ
6.82 ± 1.14C
33.03 ± 3.49B
48.21 ± 7.29A
A
–
CMeans within rows followed by the same letter are not significantly different at P
≥
0.05.
DMean aflatoxin B1 is expressed in milligrams per kilogram (n
=
5).
EAn average of 10% corn oil was added to defatted substrates.


## 3.2. Starch, Soluble Sugars, Amino Acids, and Trace Elements Contents of Different Substrates

The four main nutrients (starch, soluble sugars, amino acids, and trace elements) of defatted substrates were examined. The nutrients composition and content of six defatted substrates were very different. The starch contents of defatted wheat, corn, and corn endosperm were 67.22%, 72.21%, and 82.19%, respectively, and were significantly higher than those of the other three substrates (Table2).Table 2
Starch, trace element, soluble sugar, and amino acid contents of different substrates.
Nutrients
Defatted soybean
Defatted peanut
Defatted wheat
Defatted corn
Defatted corn endosperm
Defatted corn germ
Starch content (%)
Starch
5.26 ± 0.94E
7.01 ± 0.71E
67.22 ± 2.87C
72.21 ± 1.97B
82.19 ± 1.11A
28.12 ± 0.76D
Trace elements contents (mg/kg)
Copper
25.33 ± 9.41A
B
34.63 ± 2.44A
12.12 ± 1.90C
D
6.89 ± 0.35D
6.79 ± 1.70D
19.86 ± 3.21B
C
Iron
93.47 ± 0.22B
64.18 ± 7.68C
83.97 ± 8.51B
21.95 ± 4.15D
6.23 ± 3.36D
145.05 ± 11.31A
Zinc
51.90 ± 4.07C
84.40 ± 10.88B
39.14 ± 3.23D
18.96 ± 1.52E
12.47 ± 0.91E
107.47 ± 1.94A
Manganese
36.19 ± 0.65B
59.86 ± 1.92A
60.30 ± 0.15A
5.28 ± 0.18D
2.13 ± 0.22E
30.54 ± 0.27C
Soluble sugar concentrations (mg/g)
Fructose
3.89 ± 1.65B
C
11.12 ± 2.06A
1.94 ± 0.06C
7.72 ± 0.18A
B
1.88 ± 0.24C
9.67 ± 2.65A
Glucose
1.61 ± 0.52B
4.92 ± 1.12B
4.01 ± 0.13B
4.95 ± 0.12B
7.21 ± 0.07B
13.75 ± 4.27A
Sucrose
31.17 ± 5.05A
32.5 ± 0.46A
2.24 ± 0.12B
2.15 ± 0.33B
5.07 ± 0.1B
42.36 ± 9.19A
Maltose
0.63 ± 0.14C
0.54 ± 0.07C
4.10 ± 0.35B
4.05 ± 0.35B
7.96 ± 0.60A
1.66 ± 0.28C
Raffinose
3.32 ± 0.41B
2.08 ± 0.27B
3.00 ± 0.05B
1.11 ± 0.17B
1.16 ± 0.03B
10.71 ± 2.62A
Stachyose
34.71 ± 7.39A
6.78 ± 0.98B
0.36 ± 0.06B
NDB
NDB
0.72 ± 0.10B
Amino acid contents (%)
Aspartic acid
6.3 ± 0.38
7.70 ± 0.23
0.74 ± 0.04
0.54 ± 0.01
0.51 ± 0.15
1.81 ± 0.05
Glutamic acid
10.56 ± 0.57
13.89 ± 0.39
4.56 ± 0.24
1.72 ± 0.01
1.93 ± 0.20
3.33 ± 0.05
Glycine
2.46 ± 0.10
3.35 ± 0.15
0.55 ± 0.03
0.34 ± 0.00
0.30 ± 0.02
1.16 ± 0.02
Arginine
3.81 ± 0.18
6.95 ± 0.18
0.65 ± 0.04
0.43 ± 0.00
0.37 ± 0.03
1.73 ± 0.04
Alanine
2.58 ± 0.11
2.97 ± 0.06
0.51 ± 0.03
0.61 ± 0.01
0.66 ± 0.01
1.37 ± 0.01
Protein level
45.11 ± 2.53
55.45 ± 1.82
12.25 ± 0.37
8.36 ± 0.09
8.60 ± 1.20
19.47 ± 0.42
A–EMeans within rows followed by the same letter are not significantly different at P
≥
0.01 according to Duncan’s multiple range test (n
=
3).The concentrations of six soluble sugars (fructose, glucose, sucrose, maltose, raffinose, and stachyose) are shown in Table2. Soluble sugars were most abundant in defatted corn germ, followed by defatted soybean and peanut. Specifically, there were high concentrations of sucrose in defatted corn germ (42.36 mg/g), soybean (31.17 mg/g), and peanut (32.50 mg/g). Furthermore, the stachyose content was particularly high in defatted soybean (34.71 mg/g) (P
<
0.01).This study determined the contents of sixteen amino acids, and based on previous studies and differences in the amino acid contents, only five amino acids contents are shown in Table2; the others are not shown. The protein content was the sum of the contents of the sixteen amino acids analysed. Among the studied amino acids, glutamic acid had the highest content, followed by aspartic acid. Although the other three amino acid contents were different, these differences were not obvious compared with the contents of glutamic acid and aspartic acid. The amino acid contents of defatted peanuts and soybeans were relatively high compared with those of other grains and isolate tissues. Specifically, the glutamic acid contents of defatted soybean and peanut were 10.56% and 13.89%, respectively.The trace element contents of different grains were fairly different. The maximum content of Cu was 34.63 mg/kg in defatted peanut, and its minimum content (6.89 mg/kg) was observed in defatted corn. The maximum contents of Fe and Zn were found in defatted corn germ (145.05 and 107.47 mg/kg), and their minimum contents were identified in defatted corn endosperm. The defatted peanut and wheat were rich in Mn. Thus, the content of the four trace elements was the most abundant in defatted corn germ, followed by defatted soybean and peanut, whereas it was minimal in corn endosperm (Table2).


## 3.3. Effects of Starch, Soluble Sugars, Amino Acids, and Trace Elements on AFB1 Production

According to the solubility of the starch, this study selected five concentrations from 0.5% to 5.0%, and basal medium served as a control. These results showed that starch did not promote AFB1 production and inhibited AFB1 synthesis. The AFB1 content of control was 194.4 ng/30 mL, but in the five treatments, the maximum content of AFB1 was only 7.64 ng/30 mL which was found in 2.0% starch.The effect of soluble sugars on AFB1 production is shown in Table 3. The soluble sugar addition concentrations depend on the contents of the six substrates. These results indicated that low concentrations of soluble sugars could not promote AFB1 biosynthesis; however, when the soluble sugar concentration reached 3.0% and 6.0%, the soluble sugars promoted AFB1 production somewhat, including glucose, sucrose, and maltose (P
<
0.05). When the concentration was 3.0%, sucrose exhibited the highest promoting effect on AFB1 production (39782.61 ng/30 mL), followed by maltose (23687.29 ng/30 mL) and fructose, which had the smallest effect. However, when the concentration was 6.0%, the enhancing effects of sucrose (4471.97 ng/30 mL), raffinose (5.50 ng/30 mL), and stachyose (120.61 ng/30 mL) were reduced. Maltose promoted aflatoxin production to the greatest extent (74848.68 ng/30 mL), followed by glucose (35860.57 ng/30 mL). Among the six soluble sugars, raffinose exhibited the weakest enhancement.Table 3
Effect of soluble sugars on AFB1 production.
AflatoxinB
1
D
Concentration of soluble sugars (%)
0.5
1.0
3.0
6.0
Fructose
46.92 ± 11.30B
7.54 ± 0.83B
5.41 ± 0.56B
1137.56 ± 308.79A
Glucose
28.77 ± 3.33B
8.92 ± 0.77B
5664.33 ± 570.70B
35860.57 ± 7028.16A
Sucrose
4.63 ± 1.26B
4.28 ± 0.60B
39782.61 ± 5896.87A
4471.97 ± 442.68B
Maltose
2.84 ± 0.85C
2.34 ± 0.33C
23687.29 ± 5777.01B
74848.68 ± 8422.06A
Raffinose
5.70 ± 1.13B
2.76 ± 0.65B
208.27 ± 24.28A
5.50 ± 1.21B
Stachyose
17.02 ± 1.22B
10.08 ± 1.39B
5426.44 ± 292.49A
120.61 ± 16.55B
A–CMean values with different letters for each soluble sugar are significantly different (P
<
0.05).
DAflatoxin B1 values are the average of five replications with standard deviation in ng/30 mL.The amounts of amino acid added in this study were based on the concentrations used by Payne and Hagler Jr. [18] and amino acid concentrations of the substrates (Table 2). As the amount of amino acids increased, aflatoxin synthesis decreased (Figure 1). However, only 0.5% glutamic acid, aspartic acid, and glycine significantly stimulated AFB1 production (P
<
0.05), whereas all concentrations of arginine significantly stimulated AFB1 production (P
<
0.05). Moreover, 0.5 and 2.0% alanine reduced AFB1 production.Figure 1
Effects of glutamic acid (a), aspartic acid (b), arginine (c), alanine (d), and glycine (e) on AFB1 accumulation (ng/30 mL) at concentrations of 0, 0.5, 1.0, and 2.0 g/100 mL with incubation on a constant temperature shaker (30°C) for 5 days. The data represent the mean ± SD. Different superscript letters represent significant differences at P
<
0.05, and all treatments consisted of five replicates.
(a)
(b)
(c)
(d)
(e)Different trace elements were selected to obtain different gradient concentrations depending on the trace element contents of the six substrates (Table4). Only zinc significantly promoted AFB1 biosynthesis, and as the zinc concentration increased, AFB1 accumulation also increased. When the concentrations of zinc were 20, 50, and 100 mg/L, the contents of AFB1 were increased 4-, 5-, and 19-fold, respectively. In addition, the other three trace elements significantly inhibited AFB1 production (P
<
0
.
05) at all concentrations examined.Table 4
Effect of trace elements on AFB1 production.
AflatoxinB
1
D
Control
Concentration of trace elements (mg/L)
5
10
20
50
100
200
Copper
238.35 ± 36.61A
4.39 ± 0.29B
4.90 ± 0.59B
3.00 ± 0.94B
4.31 ± 0.08B
—
—
Iron
238.35 ± 36.61A
—
—
2.39 ± 0.08B
2.71 ± 1.21B
0.36 ± 0.23B
0.73 ± 0.20B
Zinc
238.35 ± 36.61C
—
—
954.95 ± 21.15B
C
1232.08 ± 312.71B
4733.88 ± 795.24A
—
Manganese
238.35 ± 36.61A
—
1.75 ± 0.14B
17.87 ± 3.53B
29.30 ± 4.87B
10.00 ± 3.18B
—
—: concentration not studied.
A–CMean values with different letters for each trace element are significantly different (P
<
0.05).
DAflatoxin B1 values are the average of five replications with standard deviation in ng/30 mL.


## 3.4. Effects of Starch, Soluble Sugars, Amino Acids, and Trace Elements onA. flavus Growth

The dry weights of the starch samples indicated that when more starch was present, mycelial growth was enhanced (data not shown). The dose relationship between the mycelial growth and soluble sugars revealed that higher concentrations of soluble sugars resulted in improved mycelial growth. The growth-promoting effect of stachyose was obvious, and 3.0 and 6.0% stachyose increased mycelial growth more than 2-fold compared to the other soluble sugars (Figure2). The effects of amino acids onA. flavus were also evaluated (Figure 3). Glutamic acid, aspartic acid, arginine, and alanine stimulated mycelial growth as their concentrations increased. Low concentrations of glycine promoted mycelial growth, but no significant effect was observed with 2.0% glycine. All studied trace elements, except copper, were able to promoteA. flavus mycelial growth to varying degrees. In contrast, copper significantly reduced mycelial growth at four different concentrations (Figure 4).Figure 2
Effects of soluble sugars onA. flavus growth. Different superscript letters represent significant differences at P
<
0.05 for each soluble sugar. Error bars show standard deviations of the results of five replications.Figure 3
Effects of amino acids onA. flavus growth. The data represent the mean ± SD. Letters indicate statistically significant differences (P
<
0.05) for each amino acid, and all treatments consisted of five replicates.Figure 4
Effects of copper (a), iron (b), zinc (c), and manganese (d) onA. flavus growth. Each treatment was grown as described in Section 2. The data represent the mean ± SD. Different superscript letters represent significant differences at P
<
0.05, and all treatments consisted of five replicates.
(a)
(b)
(c)
(d)


## 4. Discussion

There is a close relationship between nutrients and AFB1 production in different grains. This study verified that lipids modulate AFB1 production byA. flavus NRRL 3357. Removal of lipids from ground substrate significantly reduced the substrate’s potential for AFB1 production. Furthermore, reconstituting the defatted substrates with corn oil restored the AFB1 production. In addition, full-fat substrates, which contain abundant oil, can significantly stimulate AFB1 production. These results were consistent with those of previous studies, which provided evidence that oil-rich crops are often infected by toxigenic fungi and that lipid contents in seeds contribute to determining the severity of AFB1 contamination [10, 11, 28, 35].Although lipids are one of the most important factors supporting AFB1 biosynthesis, other nutrients also play key roles. There were big differences between the nutrients composition and content in six defatted substrates. This study found that different nutrients exert different effects on AFB1 production byA. flavus NRRL 3357 and that sucrose, glucose, maltose, arginine, glutamic acid, aspartic acid, and zinc significantly stimulated AFB1 production byA. flavus NRRL 3357. In fact, soluble sugar utilization drives AFB1 biosynthesis. In this study, when the soluble sugar concentration was increased, the AFB1 concentration also increased. When the concentrations reached 3.0% and 6.0%, soluble sugars substantially promoted AFB1 production, especially sucrose, glucose, and maltose. Similarly, Abdollahi and Buchanan [36] found that glucose and sucrose could induce AFB1 production. Manda et al. [37] also found that glucose and sucrose were positively correlated with total aflatoxins and also observed a significant positive correlation between AFB1 and sucrose. Furthermore, this study found that the contents of six soluble sugars, especially sucrose, were abundant in defatted corn germ, soybean, and peanut and that these contents correlated with AFB1 contamination. In addition, Uppala et al. [38] revealed that increasing the sugar content (specifically sucrose) of the media resulted in greater AFB1 production byA. flavus. Thus, sucrose, glucose, and maltose readily support AFB1 biosynthesis in liquid media and may also play significant roles in the AFB1 contamination of grain substrates. In six substrates, the content of glutamic acid was the highest, followed by aspartic acid and arginine, and these three amino acids were observed to promote AFB1 production byA. flavus NRRL 3357. Furthermore, the concentrations of glutamic acid and aspartic and arginine acid in defatted peanut and soybean were higher than those in other defatted substrates. Although Mellon et al. [12] indicated thatA. flavus does not appear to favour the use of storage proteins as a carbon substrate and that storage proteins are the last to be utilized by fungi [28, 39], our results revealed that glutamic acid and aspartic acid could induce AFB1 production; particularly, the study found a new factor, arginine acid, significantly stimulated AFB1 production. This discrepancy may be attributed to the different experimental conditions and substrates used. In addition to soluble sugars and amino acids, trace elements also have a substantial effect on AFB1 production. Zinc significantly stimulated AFB1 biosynthesis. Similarly, Gupta and Venkitasubramanian [40] reported that the addition of zinc to both autoclaved and nonautoclaved soybeans promoted aflatoxin production and indicated that Zn2+ is specifically required for aflatoxin biosynthesis byA. flavus [41, 42]. Furthermore, at least twenty enzymes have been found to be zinc dependent [43], which may partly account for its key role in AFB1 biosynthesis. Thus, analysing the trace elements revealed that defatted corn germ contained abundant zinc, followed by defatted soybean and peanut; however, the contents in maize and corn endosperm were very low, and these values were closely correlated with AFB1 contamination. Thus, zinc content may be one of the main causes underlying the varying degrees of contamination of these grains byA. flavus.Compared to the seven nutrients mentioned above, other nutrients did not significantly stimulate AFB1 production byA. flavus NRRL 3357 (fructose, raffinose, stachyose, alanine, and glycine) and may even inhibit AFB1 production (iron, copper, manganese, and starch). Raffinose and fructose do not clearly stimulate AFB1 production, which is in contrast to findings obtained by Mellon and Cotty [44] and Manda et al. [37], most likely because of the different strains and medium used. In addition, Tiwari et al. [45] found that iron, copper, and manganese sulfate decreased the aflatoxin production to different levels, consistent with our results. While enhanced aflatoxin production was previously observed by Lijinsky [46] in endosperm and defatted corn supplemented with Mn2+ and Cu2+, this result was not consistent with those obtained in our study, possibly because of the use of in vitro experiments and trace element ions in our study. In addition, most trace elements present in grains occur in bound forms. Although starch is abundant in corn, corn endosperm, and wheat, it does not to appear induce AFB1 biosynthesis. It is possible that the high starch content will change the characteristics of the ground substrates and that the hygroscopic nature of starch granules impedes water acquisition by the fungus [12].These nutrients not only affected AFB1 production byA. flavus NRRL 3357 but also modulatedA. flavus growth. Starch and all types of soluble sugars, amino acids, and trace elements increasedA. flavus growth, except copper, and stachyose exhibited the most significant effect. Uppala et al. [38] reported that increasing the sugar content in the media increased the mycelial growth. Moreover, many amino acids, such as asparagine, proline, and hydroxyproline, supported better mycelial growth [18]. Additionally, because increased mycelial growth is generally associated with increased toxin production [18], mycelial growth may increase the AFB1 production capacity. Thus, these results are consistent with reports of AFB1 contamination in various substrates. Indeed, soluble sugars, trace elements, and amino acids are abundant in defatted corn germ, peanut, and soybean.A. flavus is a causal agent of aspergillosis and the second-most-common pathogen responsible for invasive and noninvasive aspergillosis [47]. In addition, farmers infected by breathing in spores from contaminated foods and feeds have been reported worldwide [48] and animals can also be infected byA. flavus [49]. Thus, mycelial growth not only promotes AFB1 production but also threatens human and animal health.In conclusion, the study systematically researched the effects of nutrients on AFB1 accumulation and found the relationship between the various nutrients in grains and AFB1 contamination levels in various grains and isolate tissues. In addition to lipids, other nutrients in substrate also play key roles in AFB1 biosynthesis and mycelial growth. Maltose, glucose, sucrose, arginine, glutamic acid, aspartic acid, and zinc significantly stimulate AFB1 biosynthesis. All types of soluble sugars, amino acids, and trace elements increase mycelial growth, except copper, as evidenced by the strong relationship observed for stachyose. According to the nutrient contents and their enhancing abilities for AFB1 production byA. flavus NRRL 3357 andA. flavus growth, in addition to lipids, sucrose, stachyose, glutamic acid, and zinc may play key roles in the differential AFB1 contamination of various grains and isolate tissues. Particularly, we found that a new nutrient (arginine) of grains significantly stimulates AFB1 production and a new nutrient (stachyose) significantly stimulatesA. flavus growth. Although saccharides are utilized byA. flavus first, followed by triglycerides and finally protein [12, 28, 39], the interactions between these nutrients remain poorly understood. Thus, further studies should focus on the effects of the interactions between these nutrients on AFB1 production byA. flavus.



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*Source: 7232858-2016-05-17.xml*