# Advanced Nanocomposite Coatings of Fusion Bonded Epoxy Reinforced with Amino-Functionalized Nanoparticles for Applications in Underwater Oil Pipelines

Authors: Patricia A. Saliba; Alexandra A. P. Mansur; Herman S. Mansur
Journal: Journal of Nanomaterials
 (2016)

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## Abstract

The performance of fusion-bonded epoxy coatings can be improved through advanced composite coatings reinforced with nanomaterials. Hence, in this study a novel organic-inorganic nanocomposite finish was designed, synthesized, and characterized, achieved by addingγ-aminopropyltriethoxysilane modified silica nanoparticles produced via sol-gel process in epoxy-based powder. After the curing process of the coating reinforced with nanoparticles, the formation of a homogenous novel nanocomposite with the development of interfacial reactions between organic-inorganic and inorganic-inorganic components was observed. These hybrid nanostructures produced better integration between nanoparticles and epoxy matrix and improved mechanical properties that are expected to enhance the overall performance of the system against underwater corrosion.


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## Body

## 1. Introduction

Nanotechnology is growing at a very rapid pace in practically all fields of science and technology such as materials engineering, biomedicine, mechanics, textiles, cosmetics, agriculture, food packaging, optoelectronic and semiconductor devices, aerospace, construction, catalysis, and dentistry. Essentially, it involves the design, synthesis, characterization, and application of materials, components, and devices on the nanometer scale, that is, at sizes below 100 nm. At the nanoscale, physical, chemical, and biological properties significantly differ from the properties of individual atoms and molecules and from bulk matter, which accounted for the quantum confinement regime. Therefore, it provides a unique opportunity to develop new classes of materials, by tuning their properties and responses of living and nonliving matter, which should meet the challenges and requirements of advanced applications [1]. In the large field of nanotechnology, polymer matrix based nanocomposites have become a prominent area of current research and development [1, 2]. Polymer-based (nano)composites have been intensively studied in recent decades, which are commonly made of organic polymer matrices mostly combined with inorganic fillers. Fundamentally, the composites aim at merging the beneficial properties of inorganic materials (e.g., thermal and mechanical stabilities) and of organic polymer (e.g., light weight, flexibility, ductility, and processability) for producing innovative materials with superior properties distinct from the pristine components [3]. But the main properties of the polymer-based composites are inherently affected by the volume fraction, dimensions, shapes, and morphological aspects of the inorganic fillers. Therefore, the incorporation of the nanosized inorganic components leads to a dramatic increase in the interfacial area, which assigns unique properties to the polymer nanocomposites as compared with the ordinary composites. Amongst several alternatives of polymers for producing nanocomposites, epoxies as thermosetting polymers have been widely used as coatings, adhesives, electronic devices packing, medical devices, optical components, and structural composites due to their superior properties and low cost. However, the intrinsic brittle nature of most high-performance epoxy polymers requires the incorporation of reinforcing agents for improving the toughness and other properties. Several strategies have been explored for improving the properties of (nano)composites including the incorporation of inorganic particles, rubber particles, fibers, glass beads, microvoids, hyperbranched polymers, thermoplastic particles, and combinations of these materials [4]. In that sense, nanosized silica particle is the most commonly used nanoparticle (NP) in the preparation of nanocomposites because of its set of advantageous properties [1]. Nowadays, silica nanoparticles in the forms of amorphous or crystalline possess innumerous applications as nanomaterials in the fields of catalysis, paint stabilization, food industry, electronics, and sensors. These nanoparticles can be used in combination with polymeric matrices forming composites for improving the thermal resistance and electrical and mechanical properties, enhancing the chemical stability and tribological properties. However, due to their very small dimensions, large surface area/volume ratio, and high surface free energy, nanoparticles tend to be agglomerated reducing their dispersion in the polymeric matrices and, therefore, affecting the overall properties of the nanocomposite [5]. Thus, many efforts have been exerted to overcome this problem and to improve interactions between NPs and different polymeric matrixes. For instance, the surface of silica nanoparticles can be modified in order to achieve an appropriate dispersion in the polymeric media, as well as better functionality. Grafting of silane coupling agents on the surface of silica nanoparticles to increase the mechanical properties of nanocomposites has been the subject of numerous investigations [1]. Depending on hydrophilic or hydrophobic nature of the silane coupling agent, the surface characteristics of the modified nanoparticles can be different. The hydrophilic nature of nanoparticles increases the surface energy and reduces its wettability by a polymeric matrix [5, 6].Epoxy-based composite coatings are the oldest and still one of the largest classes of thermosetting powder coatings, which consists of a mixture of a primary resin (e.g., bisphenol-A diglycidyl ether, DGEBA), a crosslinker (e.g., polyamines), and in some cases, with the incorporation of inorganic fillers [7]. It is generally applied to the substrate, usually metal, and fused to a continuous film by baking, at temperatures in the range of 140–240°C. The performance of protective coatings is frequently limited by durability of the metal/polymer interface under harsh environment (i.e., wet, corrosive, abrasive, etc.). Fusion bonded-epoxy (FBE) powder coatings, which were first developed by 3M Co., are broadly used as high-performance composite coatings when long-term corrosion protection and thermochemical stability are critical such as in the oil, metal, gas, and water pipelines industries [8–11]. This coating has proven to be a cost-effective material for increasing the service life of reinforced structures. Yet, the performance requirements for FBE powder coatings are challenging because of their high crosslinking density. The inherent brittleness of cured coatings is one of the major obstacles restricting several applications for epoxies in a broader range of industries. Therefore, many methods have been used for improving the toughness of composite epoxy systems, including introducing nanoscale reinforcements to these materials, a possible way to achieve improved mechanical properties.However, surprisingly, although few papers have investigated the epoxy composite-steel interfacial properties [9, 12], no published report was found in consulted literature using a nanotechnology approach, where chemically modified silica nanoparticles were used as multifunctional nanoreinforcements for FBE epoxy powder coatings and the morphological and structural features were systematically characterized.Hence, in this study, a novel organic-inorganic nanocomposite coating was designed, synthesized, and characterized, achieved by adding chemically modified silica nanoparticles (NPs) withγ-aminopropyltriethoxysilane (3-APTES) producedvia sol-gel process into FBE epoxy powder, aiming at producing advanced nanocomposite coatings, which might lead to relevant improvements in performance for submarine steel oil pipelines.


## 2. Materials and Methods

### 2.1. Materials

Tetraethyl orthosilicate (TEOS, 98%, Si(OC2H5)4), (3-aminopropyl)triethoxysilane (3-APTES, 99%, H2N(CH2)3Si(OC2H5)3), and ammonium hydroxide (28–30%, NH4OH) were purchased from Sigma Aldrich (USA). Ethanol (99.5%, C2H6O), potassium hydroxide (85%, KOH), nitric acid (64–66%, HNO3), and 2-propanol (99.5%, (CH3)2CHOH) were obtained from Synth (Brazil). All chemicals and reagents were used as received without further purification. FBE powder (Scotchkote™ 226N, 3M™, River Place Blvd., Austin, USA) was used as the coating material. Deionized (DI) water (Millipore Simplicity™) with resistivity of 18 MΩ·cm was used in the preparation of all solutions. All of the preparations and syntheses were performed at room temperature (25±2°C) unless specified otherwise.


### 2.2. Methods

#### 2.2.1. Syntheses of Bare Silica and Amino-Modified Silica Nanoparticles

In this work, the reaction of TEOS in ethanol in the presence of NH4OH as catalyst for the production of silica nanoparticles was used. The syntheses of silica nanoparticles and surface-functionalized silica nanoparticles with aminopropyl groups were performed based on a previous report by Capeletti et al. [13] with some modifications. In summary, in the case of bare silica nanoparticles, 538 μL of TEOS was solubilized in 12.2 mL of ethanol under stirring. After 30 min, 400 μL of NH4OH was added to the alcoholic TEOS solution. Then, the flask was tightly sealed and the stirring was maintained for 24 h at room temperature. The resulting material (referred to as NanoSiO2) was precipitated by centrifugation, washed 4 times by redispersion of the nanoparticles in 1 mL of ethanol using ultrasonic bath followed by centrifugation and removal of supernatant. In the sequence, nanoparticle precipitate was dried at room temperature for 48 h and grounded using mortar and pestle to remove the agglomeration. Analogously, the surface-modified silica nanoparticles were prepared with the same procedure described above, with the addition of 283 μL of 3-APTES in the flask with just synthesized silica colloidal suspension. This dispersion was maintained under continuous stirring for additional 24 h at room temperature to perform the surface functionalization. Next, the dry powder of this nanomaterial (referred to as NanoSiO2-APTES) was obtained using the same procedure described for NanoSiO2 sample. The molar ratios (related to TEOS) of reagents and precursors are showed in Table 1. Before using the SiO2 nanofiller as reinforcement material, the nanoparticles were thermally treated at 200°C or 400°C for 2 h for the thermochemical stabilization and reduction of particles agglomeration.Table 1
Concentrations and molar ratios of reagents and precursors used for the synthesis of silica-based nanoparticles.
Sample Reagent TEOS H2O NH3 EtOH 3-APTES NanoSiO2 Concentration (mol·L−1) 0.18 1.21 0.36 15.59 — Molar ratio 1.0 6.7 2.0 86.6 — NanoSiO2-APTES Concentration (mol·L−1) 0.18 1.21 0.36 15.59 0.09 Molar ratio 1.0 6.7 2.0 86.6 0.5


#### 2.2.2. Characterization of Bare Silica and Amino-Modified Silica Nanoparticles

The morphological features of the nanoparticles were characterized by scanning electron microscopy (SEM, FEI-INSPECTTM S50) coupled with energy dispersion X-ray spectroscopy (EDX, EDAX GENESIS) and transmission electron microscopy (TEM, Tecnai G2-20-FEI microscope, 200 kV). For SEM analysis, before examination, the samples were coated with a thin carbon film by sputtering using a low deposition rate, substrate cooling, and maximum distance between the target and sample in order to avoid sample damage. Images of secondary electrons (SE) were obtained with an accelerating voltage of 15 kV. The TEM samples were prepared by dropping an aliquot of ethanolic dispersion of nanoparticle samples (0.5 g L−1 ultrasonicated for 30 min) onto a holey carbon grid before analysis. The nanoparticle size distributions were obtained from the SEM and TEM images by measuring at least 100 randomly selected nanoparticles using image processing program freeware (ImageJ, version 1.50).Nanoparticles were analyzed by Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS) method (Thermo Fisher, Nicolet 6700) over the range of 400 to 4000 cm−1 using 64 scans and a resolution of 2 cm−1. The powder samples were mixed in a ratio of 1% (wt.%) to potassium bromide (Sigma Aldrich, USA, ≥99%, KBr, suitable for spectroscopy) previously dried at 110±5°C for 2 h.Thermogravimetric (TG) and differential scanning calorimetry (DSC) thermal analyses were performed using SDT Q-600 simultaneous TGA/DSC instrument (TA Instruments Co., New Castle, DE, USA). Samples of approximately6.5±1.0 mg were used for the experiments at a heating rate of 10°C·min−1 (range from 20 to 900°C). The samples were loaded into an open alumina crucible. TG and DSC curves were recorded simultaneously with 0.1 μg sensitivity. The thermal analyses were performed under the continuous flow of dry nitrogen gas (50 mL·min−1).Dynamic light scattering (DLS) measurements were performed on the colloidal suspension of nanoparticles in ethanol after the synthesis using a Brookhaven ZetaPlus instrument with a laser light wavelength of 660 nm (35 mW red diode laser) and a thermostat with temperature stabilization. Standard square quartz cells with a volume of 4.5 mL were used. Samples were measured at25±2°C and light scattering was detected at 90°. Five measurements were obtained for each system and averaged.


#### 2.2.3. Deposition and Characterization of Epoxy-Based Nanocomposite Coating Reinforced with Silica and Surface-Modified Nanoparticles

Epoxy-based FBE powder was mixed with NanoSiO2 or NanoSiO2-APTES nanoparticles (1% wt.%) previously synthesized and thermally stabilized. The powder mixtures were homogenized by mechanical stirring at 3500 rpm for 2 h. In the sequence, FBE powder and FBE reinforced with SiO2 nanoparticles (FBE-NanoSiO2 and FBE-NanoSiO2-APTES) were deposited on the solid support and the system was heated at 248±5°C for 5 min and cooled down to room temperature, as schematically represented in Figure 1.Figure 1
Schematic representation of the deposition and curing of the FBE nanocomposite coatings.The morphology of the reference FBE coating and FBE nanocomposite coatings was evaluated using scanning electron microscopy (SEM), where images were taken from freshly freeze-fractured surfaces of glass slide/coating systems with a FEI-INSPECTTM S50 microscope coupled to EDX (EDAX GENESIS) for semiquantitative chemical analysis. Before examination, samples were sputtered with carbon as previously described. Images of secondary electrons (SE) and backscattered electrons (BSE) were obtained using an accelerating voltage of 15 kV.Midinfrared spectroscopy (Thermo Fisher, Nicolet 6700) of FBE coatings was collected using attenuated total reflectance method (ATR, 4000 to 675 cm−1, 32 scans, and a 4 cm−1 resolution). In addition, Fourier transform infrared image mapping (FTIR-I) analysis of the cured coatings was performed using Nicolet-iN10 infrared microscope (Thermo Electron Corp., WI, USA) with OMNIC Picta software (Thermo Scientific, WI, USA). ATR image mappings were acquired between 4000 and 675 cm−1 with a spectral resolution of 8 cm−1. For each sample, an area of 400 μm × 400 μm was randomly selected for imaging and analysis constituting 441 points of spectral data acquisition per region analyzed.DSC analyses were performed using SDT Q-600 instrument (TA Instruments Co) to verify the transition temperature (Tg) of the cured coatings. About 6.0±1.0 mg of FBE, FBE-NanoSiO2, and FBE-NanoSiO2-APTES powders was submitted to the following thermal profile: 1st step: temperature increase at 10°C·min−1 from 25°C up to 300°C; 2nd step: cooling down to room temperature; 3rd step: temperature increase at 10°C·min−1 from 25°C up to 300°C. The experiments were carried out in aluminum crucibles with nonsealed lids under nitrogen gas flow of 50 mL·min−1. Tg was calculated as the midpoint temperature according to ASTM E1356–08 (Standard Test Method for Assignment of the Glass Transition Temperatures by Differential Scanning Calorimetry) [14].The nanoindentation study was performed on a MFP3D nanoindenter (Asylum Research) equipped with a Berkovich diamond indenter tip at room temperature. The FBE and nanocomposites were deposited on glass supports and the samples were glued on stubs. The coating thickness was approximately 350μm and the contribution of glass substrate to the final results was negligible. The maximum load was 2 mN, and the loading and unloading rate was 0.13 mN·s−1. To minimize the viscous creep effect, at the maximum load, the indenter was held for 15 s before starting unloading. A total of 36 indentations on a 50 μm × 50 μm area were applied on each sample. The curves were analyzed according to the Oliver-Pharr method [15], and the average values of hardness and elastic modulus were reported. Atomic force microscopy (AFM) was performed on the indented areas, after the nanoindentation tests, using an MFP-3D-SA SPM microscope (Asylum Research).


## 2.1. Materials

Tetraethyl orthosilicate (TEOS, 98%, Si(OC2H5)4), (3-aminopropyl)triethoxysilane (3-APTES, 99%, H2N(CH2)3Si(OC2H5)3), and ammonium hydroxide (28–30%, NH4OH) were purchased from Sigma Aldrich (USA). Ethanol (99.5%, C2H6O), potassium hydroxide (85%, KOH), nitric acid (64–66%, HNO3), and 2-propanol (99.5%, (CH3)2CHOH) were obtained from Synth (Brazil). All chemicals and reagents were used as received without further purification. FBE powder (Scotchkote™ 226N, 3M™, River Place Blvd., Austin, USA) was used as the coating material. Deionized (DI) water (Millipore Simplicity™) with resistivity of 18 MΩ·cm was used in the preparation of all solutions. All of the preparations and syntheses were performed at room temperature (25±2°C) unless specified otherwise.


## 2.2. Methods

### 2.2.1. Syntheses of Bare Silica and Amino-Modified Silica Nanoparticles

In this work, the reaction of TEOS in ethanol in the presence of NH4OH as catalyst for the production of silica nanoparticles was used. The syntheses of silica nanoparticles and surface-functionalized silica nanoparticles with aminopropyl groups were performed based on a previous report by Capeletti et al. [13] with some modifications. In summary, in the case of bare silica nanoparticles, 538 μL of TEOS was solubilized in 12.2 mL of ethanol under stirring. After 30 min, 400 μL of NH4OH was added to the alcoholic TEOS solution. Then, the flask was tightly sealed and the stirring was maintained for 24 h at room temperature. The resulting material (referred to as NanoSiO2) was precipitated by centrifugation, washed 4 times by redispersion of the nanoparticles in 1 mL of ethanol using ultrasonic bath followed by centrifugation and removal of supernatant. In the sequence, nanoparticle precipitate was dried at room temperature for 48 h and grounded using mortar and pestle to remove the agglomeration. Analogously, the surface-modified silica nanoparticles were prepared with the same procedure described above, with the addition of 283 μL of 3-APTES in the flask with just synthesized silica colloidal suspension. This dispersion was maintained under continuous stirring for additional 24 h at room temperature to perform the surface functionalization. Next, the dry powder of this nanomaterial (referred to as NanoSiO2-APTES) was obtained using the same procedure described for NanoSiO2 sample. The molar ratios (related to TEOS) of reagents and precursors are showed in Table 1. Before using the SiO2 nanofiller as reinforcement material, the nanoparticles were thermally treated at 200°C or 400°C for 2 h for the thermochemical stabilization and reduction of particles agglomeration.Table 1
Concentrations and molar ratios of reagents and precursors used for the synthesis of silica-based nanoparticles.
Sample Reagent TEOS H2O NH3 EtOH 3-APTES NanoSiO2 Concentration (mol·L−1) 0.18 1.21 0.36 15.59 — Molar ratio 1.0 6.7 2.0 86.6 — NanoSiO2-APTES Concentration (mol·L−1) 0.18 1.21 0.36 15.59 0.09 Molar ratio 1.0 6.7 2.0 86.6 0.5


### 2.2.2. Characterization of Bare Silica and Amino-Modified Silica Nanoparticles

The morphological features of the nanoparticles were characterized by scanning electron microscopy (SEM, FEI-INSPECTTM S50) coupled with energy dispersion X-ray spectroscopy (EDX, EDAX GENESIS) and transmission electron microscopy (TEM, Tecnai G2-20-FEI microscope, 200 kV). For SEM analysis, before examination, the samples were coated with a thin carbon film by sputtering using a low deposition rate, substrate cooling, and maximum distance between the target and sample in order to avoid sample damage. Images of secondary electrons (SE) were obtained with an accelerating voltage of 15 kV. The TEM samples were prepared by dropping an aliquot of ethanolic dispersion of nanoparticle samples (0.5 g L−1 ultrasonicated for 30 min) onto a holey carbon grid before analysis. The nanoparticle size distributions were obtained from the SEM and TEM images by measuring at least 100 randomly selected nanoparticles using image processing program freeware (ImageJ, version 1.50).Nanoparticles were analyzed by Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS) method (Thermo Fisher, Nicolet 6700) over the range of 400 to 4000 cm−1 using 64 scans and a resolution of 2 cm−1. The powder samples were mixed in a ratio of 1% (wt.%) to potassium bromide (Sigma Aldrich, USA, ≥99%, KBr, suitable for spectroscopy) previously dried at 110±5°C for 2 h.Thermogravimetric (TG) and differential scanning calorimetry (DSC) thermal analyses were performed using SDT Q-600 simultaneous TGA/DSC instrument (TA Instruments Co., New Castle, DE, USA). Samples of approximately6.5±1.0 mg were used for the experiments at a heating rate of 10°C·min−1 (range from 20 to 900°C). The samples were loaded into an open alumina crucible. TG and DSC curves were recorded simultaneously with 0.1 μg sensitivity. The thermal analyses were performed under the continuous flow of dry nitrogen gas (50 mL·min−1).Dynamic light scattering (DLS) measurements were performed on the colloidal suspension of nanoparticles in ethanol after the synthesis using a Brookhaven ZetaPlus instrument with a laser light wavelength of 660 nm (35 mW red diode laser) and a thermostat with temperature stabilization. Standard square quartz cells with a volume of 4.5 mL were used. Samples were measured at25±2°C and light scattering was detected at 90°. Five measurements were obtained for each system and averaged.


### 2.2.3. Deposition and Characterization of Epoxy-Based Nanocomposite Coating Reinforced with Silica and Surface-Modified Nanoparticles

Epoxy-based FBE powder was mixed with NanoSiO2 or NanoSiO2-APTES nanoparticles (1% wt.%) previously synthesized and thermally stabilized. The powder mixtures were homogenized by mechanical stirring at 3500 rpm for 2 h. In the sequence, FBE powder and FBE reinforced with SiO2 nanoparticles (FBE-NanoSiO2 and FBE-NanoSiO2-APTES) were deposited on the solid support and the system was heated at 248±5°C for 5 min and cooled down to room temperature, as schematically represented in Figure 1.Figure 1
Schematic representation of the deposition and curing of the FBE nanocomposite coatings.The morphology of the reference FBE coating and FBE nanocomposite coatings was evaluated using scanning electron microscopy (SEM), where images were taken from freshly freeze-fractured surfaces of glass slide/coating systems with a FEI-INSPECTTM S50 microscope coupled to EDX (EDAX GENESIS) for semiquantitative chemical analysis. Before examination, samples were sputtered with carbon as previously described. Images of secondary electrons (SE) and backscattered electrons (BSE) were obtained using an accelerating voltage of 15 kV.Midinfrared spectroscopy (Thermo Fisher, Nicolet 6700) of FBE coatings was collected using attenuated total reflectance method (ATR, 4000 to 675 cm−1, 32 scans, and a 4 cm−1 resolution). In addition, Fourier transform infrared image mapping (FTIR-I) analysis of the cured coatings was performed using Nicolet-iN10 infrared microscope (Thermo Electron Corp., WI, USA) with OMNIC Picta software (Thermo Scientific, WI, USA). ATR image mappings were acquired between 4000 and 675 cm−1 with a spectral resolution of 8 cm−1. For each sample, an area of 400 μm × 400 μm was randomly selected for imaging and analysis constituting 441 points of spectral data acquisition per region analyzed.DSC analyses were performed using SDT Q-600 instrument (TA Instruments Co) to verify the transition temperature (Tg) of the cured coatings. About 6.0±1.0 mg of FBE, FBE-NanoSiO2, and FBE-NanoSiO2-APTES powders was submitted to the following thermal profile: 1st step: temperature increase at 10°C·min−1 from 25°C up to 300°C; 2nd step: cooling down to room temperature; 3rd step: temperature increase at 10°C·min−1 from 25°C up to 300°C. The experiments were carried out in aluminum crucibles with nonsealed lids under nitrogen gas flow of 50 mL·min−1. Tg was calculated as the midpoint temperature according to ASTM E1356–08 (Standard Test Method for Assignment of the Glass Transition Temperatures by Differential Scanning Calorimetry) [14].The nanoindentation study was performed on a MFP3D nanoindenter (Asylum Research) equipped with a Berkovich diamond indenter tip at room temperature. The FBE and nanocomposites were deposited on glass supports and the samples were glued on stubs. The coating thickness was approximately 350μm and the contribution of glass substrate to the final results was negligible. The maximum load was 2 mN, and the loading and unloading rate was 0.13 mN·s−1. To minimize the viscous creep effect, at the maximum load, the indenter was held for 15 s before starting unloading. A total of 36 indentations on a 50 μm × 50 μm area were applied on each sample. The curves were analyzed according to the Oliver-Pharr method [15], and the average values of hardness and elastic modulus were reported. Atomic force microscopy (AFM) was performed on the indented areas, after the nanoindentation tests, using an MFP-3D-SA SPM microscope (Asylum Research).


## 2.2.1. Syntheses of Bare Silica and Amino-Modified Silica Nanoparticles

In this work, the reaction of TEOS in ethanol in the presence of NH4OH as catalyst for the production of silica nanoparticles was used. The syntheses of silica nanoparticles and surface-functionalized silica nanoparticles with aminopropyl groups were performed based on a previous report by Capeletti et al. [13] with some modifications. In summary, in the case of bare silica nanoparticles, 538 μL of TEOS was solubilized in 12.2 mL of ethanol under stirring. After 30 min, 400 μL of NH4OH was added to the alcoholic TEOS solution. Then, the flask was tightly sealed and the stirring was maintained for 24 h at room temperature. The resulting material (referred to as NanoSiO2) was precipitated by centrifugation, washed 4 times by redispersion of the nanoparticles in 1 mL of ethanol using ultrasonic bath followed by centrifugation and removal of supernatant. In the sequence, nanoparticle precipitate was dried at room temperature for 48 h and grounded using mortar and pestle to remove the agglomeration. Analogously, the surface-modified silica nanoparticles were prepared with the same procedure described above, with the addition of 283 μL of 3-APTES in the flask with just synthesized silica colloidal suspension. This dispersion was maintained under continuous stirring for additional 24 h at room temperature to perform the surface functionalization. Next, the dry powder of this nanomaterial (referred to as NanoSiO2-APTES) was obtained using the same procedure described for NanoSiO2 sample. The molar ratios (related to TEOS) of reagents and precursors are showed in Table 1. Before using the SiO2 nanofiller as reinforcement material, the nanoparticles were thermally treated at 200°C or 400°C for 2 h for the thermochemical stabilization and reduction of particles agglomeration.Table 1
Concentrations and molar ratios of reagents and precursors used for the synthesis of silica-based nanoparticles.
Sample Reagent TEOS H2O NH3 EtOH 3-APTES NanoSiO2 Concentration (mol·L−1) 0.18 1.21 0.36 15.59 — Molar ratio 1.0 6.7 2.0 86.6 — NanoSiO2-APTES Concentration (mol·L−1) 0.18 1.21 0.36 15.59 0.09 Molar ratio 1.0 6.7 2.0 86.6 0.5


## 2.2.2. Characterization of Bare Silica and Amino-Modified Silica Nanoparticles

The morphological features of the nanoparticles were characterized by scanning electron microscopy (SEM, FEI-INSPECTTM S50) coupled with energy dispersion X-ray spectroscopy (EDX, EDAX GENESIS) and transmission electron microscopy (TEM, Tecnai G2-20-FEI microscope, 200 kV). For SEM analysis, before examination, the samples were coated with a thin carbon film by sputtering using a low deposition rate, substrate cooling, and maximum distance between the target and sample in order to avoid sample damage. Images of secondary electrons (SE) were obtained with an accelerating voltage of 15 kV. The TEM samples were prepared by dropping an aliquot of ethanolic dispersion of nanoparticle samples (0.5 g L−1 ultrasonicated for 30 min) onto a holey carbon grid before analysis. The nanoparticle size distributions were obtained from the SEM and TEM images by measuring at least 100 randomly selected nanoparticles using image processing program freeware (ImageJ, version 1.50).Nanoparticles were analyzed by Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS) method (Thermo Fisher, Nicolet 6700) over the range of 400 to 4000 cm−1 using 64 scans and a resolution of 2 cm−1. The powder samples were mixed in a ratio of 1% (wt.%) to potassium bromide (Sigma Aldrich, USA, ≥99%, KBr, suitable for spectroscopy) previously dried at 110±5°C for 2 h.Thermogravimetric (TG) and differential scanning calorimetry (DSC) thermal analyses were performed using SDT Q-600 simultaneous TGA/DSC instrument (TA Instruments Co., New Castle, DE, USA). Samples of approximately6.5±1.0 mg were used for the experiments at a heating rate of 10°C·min−1 (range from 20 to 900°C). The samples were loaded into an open alumina crucible. TG and DSC curves were recorded simultaneously with 0.1 μg sensitivity. The thermal analyses were performed under the continuous flow of dry nitrogen gas (50 mL·min−1).Dynamic light scattering (DLS) measurements were performed on the colloidal suspension of nanoparticles in ethanol after the synthesis using a Brookhaven ZetaPlus instrument with a laser light wavelength of 660 nm (35 mW red diode laser) and a thermostat with temperature stabilization. Standard square quartz cells with a volume of 4.5 mL were used. Samples were measured at25±2°C and light scattering was detected at 90°. Five measurements were obtained for each system and averaged.


## 2.2.3. Deposition and Characterization of Epoxy-Based Nanocomposite Coating Reinforced with Silica and Surface-Modified Nanoparticles

Epoxy-based FBE powder was mixed with NanoSiO2 or NanoSiO2-APTES nanoparticles (1% wt.%) previously synthesized and thermally stabilized. The powder mixtures were homogenized by mechanical stirring at 3500 rpm for 2 h. In the sequence, FBE powder and FBE reinforced with SiO2 nanoparticles (FBE-NanoSiO2 and FBE-NanoSiO2-APTES) were deposited on the solid support and the system was heated at 248±5°C for 5 min and cooled down to room temperature, as schematically represented in Figure 1.Figure 1
Schematic representation of the deposition and curing of the FBE nanocomposite coatings.The morphology of the reference FBE coating and FBE nanocomposite coatings was evaluated using scanning electron microscopy (SEM), where images were taken from freshly freeze-fractured surfaces of glass slide/coating systems with a FEI-INSPECTTM S50 microscope coupled to EDX (EDAX GENESIS) for semiquantitative chemical analysis. Before examination, samples were sputtered with carbon as previously described. Images of secondary electrons (SE) and backscattered electrons (BSE) were obtained using an accelerating voltage of 15 kV.Midinfrared spectroscopy (Thermo Fisher, Nicolet 6700) of FBE coatings was collected using attenuated total reflectance method (ATR, 4000 to 675 cm−1, 32 scans, and a 4 cm−1 resolution). In addition, Fourier transform infrared image mapping (FTIR-I) analysis of the cured coatings was performed using Nicolet-iN10 infrared microscope (Thermo Electron Corp., WI, USA) with OMNIC Picta software (Thermo Scientific, WI, USA). ATR image mappings were acquired between 4000 and 675 cm−1 with a spectral resolution of 8 cm−1. For each sample, an area of 400 μm × 400 μm was randomly selected for imaging and analysis constituting 441 points of spectral data acquisition per region analyzed.DSC analyses were performed using SDT Q-600 instrument (TA Instruments Co) to verify the transition temperature (Tg) of the cured coatings. About 6.0±1.0 mg of FBE, FBE-NanoSiO2, and FBE-NanoSiO2-APTES powders was submitted to the following thermal profile: 1st step: temperature increase at 10°C·min−1 from 25°C up to 300°C; 2nd step: cooling down to room temperature; 3rd step: temperature increase at 10°C·min−1 from 25°C up to 300°C. The experiments were carried out in aluminum crucibles with nonsealed lids under nitrogen gas flow of 50 mL·min−1. Tg was calculated as the midpoint temperature according to ASTM E1356–08 (Standard Test Method for Assignment of the Glass Transition Temperatures by Differential Scanning Calorimetry) [14].The nanoindentation study was performed on a MFP3D nanoindenter (Asylum Research) equipped with a Berkovich diamond indenter tip at room temperature. The FBE and nanocomposites were deposited on glass supports and the samples were glued on stubs. The coating thickness was approximately 350μm and the contribution of glass substrate to the final results was negligible. The maximum load was 2 mN, and the loading and unloading rate was 0.13 mN·s−1. To minimize the viscous creep effect, at the maximum load, the indenter was held for 15 s before starting unloading. A total of 36 indentations on a 50 μm × 50 μm area were applied on each sample. The curves were analyzed according to the Oliver-Pharr method [15], and the average values of hardness and elastic modulus were reported. Atomic force microscopy (AFM) was performed on the indented areas, after the nanoindentation tests, using an MFP-3D-SA SPM microscope (Asylum Research).


## 3. Results and Discussion

### 3.1. Characterization of Bare Silica and Amino-Modified Silica Nanoparticles

The sol-gel process is widely used to produce pure silica particles due to the ability of this method to control the particle size, size distribution, and morphology through systematic monitoring of reaction parameters. The process involves hydrolysis and condensation of silicon alkoxides (Si(OR)4) such as tetraethylorthosilicate (TEOS, Si(OC2H5)4) in the presence of mineral acid (e.g., HCl) or base (e.g., NH3) as catalyst [1]. In this study, the sol-gel process was used to synthesize pure silica and organically modified silica nanoparticles for reinforcement of epoxy-based nanocomposite coatings. The TEM results presented in Figure 2 evidenced that the silica nanoparticles were spherical and reasonably monodispersed with the average size of 53±13 nm, where no significant effect of the amino functionalization was detected on the morphological aspects and size distribution of the silica nanoparticles. In addition, the EDX chemical analysis showed silicon and oxygen as the predominant elements, besides the other constituents of the grid (e.g., copper and carbon). These results demonstrated that nanosized silica particles were successfully produced with dimensions within controllable rangevia facile sol-gel processing route. It is worth highlighting that the properties of nanomaterials are usually size dependent, which can be designed and produced by controlling the size distribution of the nanoparticles by sol-gel processing route [1]. In addition, the amount of atoms residing on the surface increases with the decrease in particle size, which affects the amount of chemical groups present on the surface, such as silanols (-Si-OH) and incorporated functional species (i.e., -NH2). Thus, the combination of size and chemical functionalities of silica nanoparticles assigns unique properties to the resulting nanocomposites reinforced by these nanomaterials.Figure 2
(a) Typical TEM image of silica nanoparticles. (b) EDX spectrum of silica nanoparticles. (c) Nanoparticles size distribution.
(a) (b) (c)FTIR is used as a powerful tool for accessing the chemical groups during the synthesis after the formation of silica particles and the incorporation of functional groups at the surfaces. In Figure3 the FTIR spectra of bare silica (a) and amino-modified silica nanoparticles (b) are presented. As a general trend, in both spectra the major chemical species associated with the sol-gel synthesis of silica nanoparticles is clearly observed, such as stretching vibration bands of Si-O-Si at 1130–1000 cm−1 and 800 cm−1. In addition, the stretching vibration bands of Si-OH groups at 975–865 cm−1 related to the incomplete condensation of silanols. The broad band at 3700–3200 cm−1 is predominantly assigned to the stretching band of hydroxyl groups (Si-OH) in the structure and at the surface of the silica nanoparticles. In particular, the FTIR spectrum of Figure 3(b) shows the vibration bands at 3500–3200 cm−1 associated with amine groups (-NH2) overlapped with signals from silanol groups and the bands at 1573 cm−1 and 1318 cm−1 that are associated with the in-plane scissoring bending of primary amines (-NH2) and the stretching of -CN bonds, respectively [16]. Moreover, alkyl bands (-CH2) were detected at 2925, 2853 cm−1, and 1465 cm−1, which were assigned to the stretching and bending contributions of propyl groups present in the organosilane used as chemical surface modifier. Hence, these results evidenced that silica nanoparticles were produced by the sol-gel process with silanol groups incorporated in the structure. Furthermore, it was possible to chemically modify the system by adding amino-derived organosilane precursor during the sol-gel synthesis, leading to the formation of surface-functionalized silica nanoparticles. These aspects are relevant as far as the reinforcement of composite with nanoparticle is concerned because they offer chemical functional groups for the reaction with the epoxy-based matrix, which can potentially change the overall properties of the system.Figure 3
FTIR spectra of (a) silica nanoparticles and (b) silica nanoparticles modified by 3-APTES.Thermogravimetric (TG) analyses of the silica nanoparticles are presented in Figure4. TG analyses showed two-stage mass losses for bare silica nanoparticles (regions A and B) and three stages for amino-functionalized silica samples (3-APTES, regions A, B, and C) as shown in Figures 4(a) and 4(b), respectively. Essentially, for both silica samples (a and b) significant mass losses of approximately 8% below the temperature of 144°C (region A, Figure 4) can be observed, which were assigned to the drying process by evaporation and desorption of liquids, that is, ethanol and water molecules, from the silica nanoparticulate system [17]. The second stage of mass loss at region B of approximately 2%, between 144°C and 455°C (Figure 4), was predominantly associated with the release of trapped water within the pore, degradation of residual unreacted precursors (e.g., alcohol, ammonia, and silane), and dehydroxylation of silanol groups (Si-OH) and formation of siloxane linkage (Si-O-Si) [18]. Finally, from 455°C to 900°C (region C) silica nanoparticles presented just minor gradual mass loss (~1%) mostly due to the further increase in siloxane bonds and reduced surface hydroxyl groups. However, the amino-modified nanoparticles showed significant additional mass loss of approximately 4%, as a result of the degradation of the aminopropyl functional groups at the surface of the silica nanoparticles. The identification of these stages is very important because they evidenced the transition temperatures and range of temperature required for posterior thermal treatment of the silica nanoparticles after the synthesis in order to promote their densification and thermochemical stabilization. This aspect is crucial for utilizing these silica nanoparticles as inorganic reinforcements of epoxy-based nanocomposites. Therefore, based on the aforementioned reasons, this study used the heat thermal treatment of the silica nanoparticles (bare and amino-modified) at temperatures 200°C and 400°C in order to promote their thermochemical stabilization prior to the incorporation in the epoxy matrix. The curing temperature of the epoxy matrix was 248°C, much lower than the temperature of the thermally stabilized silica nanoparticles avoiding any degradation, which would negatively affect the properties of the FBE nanocomposite coatings.Figure 4
Thermal analyses of silica nanoparticles (a) before and (b) after surface functionalization with 3-APTES.The freshly synthesized silica nanoparticles (in gel form) were dried under heat drying in oven for 2 h. Drying process can cause the decrease in gel volume due to the loss of liquid by evaporation through the silica pore. Conversely, silica nanoparticles tend to coalesce upon heating, which can lead to changes in the average size of the nanoparticles. In this study, particle size distribution of the two silica nanoparticulate systems, with and without surface chemical functionalization, showed no significant statistical difference before and after the thermal treatment at 200°C and 400°C. SEM images (Figure5) reveal a relative narrow size distribution and sphere-like shape on the morphological aspects of the nanosize particles with roughly intermediate aggregation and agglomeration “as synthesized” (Figures 5(a) and 5(b)) due to presence of interparticle moisture. After heating at the temperatures of 200°C (Figures 5(c) and 5(d)) and 400°C (Figure 5(e)) a relative gradual reduction of agglomerates was observed, leading to more uniformly dispersed nanoparticulate systems mostly caused by the removal of water molecules upon heating and, therefore, decreasing the attractive hydrogen bonds between silica nanoparticles [19].Figure 5
SEM images of silica nanoparticles “as synthesized” ((a) nonfunctionalized and (b) amino-functionalized) and after heating at 200°C ((c) nonfunctionalized and (d) amino-functionalized) and at 400°C ((e) nonfunctionalized).
(a) (b) (c) (d) (e)The broad range of applications of silica nanoparticles is often determined by the particle size in the colloidal media. Dynamic light scattering (DLS) is a characterization technique that provides fast, accurate, and repeatable nanoparticle size data and therefore is an essential tool for the preliminary investigation of silica nanoparticles produced via sol-gel chemistry and the effect of surface functionalization by organic species. Hence, in this study, DLS measurements were performed in order to evaluate the hydrodynamic radius (HD) of the silica nanoparticles as synthesized and after surface functionalization with aminopropyl silane. The results are presented in Figure 6, with average HD values of 93±9 nm and 152±65 nm (polydispersity index = 0.032) for bare silica nanoparticles (Figure 6(a)) and APTES-silica nanoparticles (Figure 6(b)), respectively. These size distribution plots indicated the formation of reasonably monodispersed systems [20]. In addition, the results demonstrated the effective chemical functionalization of the silica nanoparticle surfaces, which caused the drastic increase in HD of the silica-modified nanoparticles (i.e., interparticle spacing), because of the steric hindrance and repulsion of hydrophobic aminopropyl groups at the surfaces dispersed in the hydrophilic water medium. The suggested schematic representation of the silica nanoparticles colloidal systems synthesized in this work is depicted in Figure 7.Figure 6
DLS measurements of silica nanoparticles (a) before and (b) after surface functionalization with 3-APTES. (c) Schematic representation of surface interaction of silica nanoparticles in water medium.Figure 7
Schematic representation of nanoparticle chemical structure: (a) NanoSiO2 and (b) NanoSiO2-APTES.
(a) (b)


### 3.2. Characterization of Epoxy-Based Nanocomposite Coating Reinforced with Silica and Surface-Modified Nanoparticles

Epoxy resin of Scotchkote 226N is based on poly(bisphenol A-co-epichlorohydrin) end-capped glycidyl (DGEBA, Figure8(a)) and dicyandiamide (DDA, Figure 8(b)) as the curing agent. Despite the complex mechanisms and reactions involved the curing process starts with the diffusion of the dicyandiamide into the epoxy resin and the reaction between the oxirane rings of DGEBA and primary and secondary amine groups of the dicyandiamide breaking the oxirane ring and forming hydroxyl groups. As the reaction proceeds, the formed hydroxyl groups can react with cyano and oxirane groups leading to the formation of a highly crosslinked network [21–23].Figure 8
Chemical structures of DGEBA (a) and DDA (b).
(a) (b)SEM images obtained from freeze-fractured surfaces of coatings are shown in Figure9. BSE image of FBE coating (Figure 9(a)) indicated that it is a composite material with inorganic particles (bright) immersed in the polymeric matrix (dark). As reported in a previous study developed by our group [9], the inorganic filler of FBE used in this study consists of small rounded anatase (TiO2) particles and needle-shaped wollastonite (CaSiO3) with different grades of aspect ratio in an amount of 32.8±3.0 wt.%. Morphologies of FBE nanocomposites reinforced with NanoSiO2 (Figure 9(b)) and NanoSiO2-APTES (Figure 9(c)) were similar to those observed for original FBE with only small agglomerates of silica nanoparticles (red arrows), with dimensions smaller than the calcium silicate nanoparticles. For all coating samples, secondary electron images of fractured materials (Figures 9(d), 9(e), and 9(f)) revealed a surface with distracted patterns that is characteristic of epoxy matrix reinforced with particles. This feature is usually associated with the presence of well bonded filler materials causing local yielding of the epoxy matrix, in contrast to the smooth and glassy brittle fracture surface of pure epoxy reported in literature [24]. In this sense, the addition of nanofiller into the FBE composite did not significantly affect the fracture surface, probably due to the small amount of SiO2 nanoparticles (1.0 wt.%) incorporated in epoxy-based material that already presented microsized inorganic fillers. The main difference between the FBE composite and the FBE nanocomposites (FBE-NanoSiO2 and FBE-NanoSiO2-APTES) is the presence of some voids in the epoxy matrix due to the presence of residual volatile components (Figures 9(e) and 9(f), white arrows) [18, 25]. Based on the thermal analysis of the silica nanoparticles presented in Section 3.1, there is still a loss of mass associated with the condensation of silanol groups, release of trapped water, and degradation of residual unreacted precursors at curing temperature (248±5°C) that is higher than the temperatures of 200°C used to stabilize and disperse the nanoparticles. On the other hand, no voids were observed for silica nanoparticles thermally treated at 400°C (Figure 10).Figure 9
Morphological analysis of FBE coatings (a and d) FBE, (b and e) FBE-NanoSiO2, and (c and f) FBE-NanoSiO2-APTES (red arrows, SiO2 agglomerates, and white arrows, voids).
(a) (b) (c) (d) (e) (f)Figure 10
Typical SE image of FBE-NanoSiO2-APTES coating with nanoparticles heat dried at 400°C.Based on precursors and main reactions involved in the formation of crosslinked epoxy matrix, midinfrared spectrum of cured FBE presented vibration bands associated with hydroxyl groups, oxirane ring, methyl and methylene groups, and C=C and C-C bonds in aromatic structures, besides ether and primary and secondary amines as depicted in Figure11 [21, 22, 26, 27]. In addition, bands associated with the silicate mineral filler (Si-O-Si and Si-O-Ca) were also observed.Figure 11
FTIR spectra of (A) FBE, (B) FBE-NanoSiO2, and (C) FBE-NanoSiO2-APTES in the spectral range of 4000–2500 cm−1 (a) and 1800–675 cm−1 (b).
(a) (b)To perform a deeper analysis, the curing extent of each system (FBE, FBE-NanoSiO2, and FBE-NanoSiO2-APTES) was evaluated by the absorbance intensity (A) of two bands related to oxirane ring (830 cm−1 and 3060 cm−1) normalized using the absorbance intensity of the C=C band of phenyl ring at 1608 cm−1 that is not involved in curing reactions remaining constant throughout [28]. The oxirane ring bands decrease upon curing as a consequence of the epoxy ring opening and, as the relative intensity of FTIR band is proportional to the relative concentration of the components in a sample, the results of the ratio ofA830/A1608 andA3060/A1608 (Figures 12(a) and 12(b), resp.) revealed that the FBE-NanoSiO2-APTES presented a higher degree of consumption of epoxy resin followed by FBE and FBE-NanoSiO2. FTIR image maps (FTIR-I analysis) of oxirane band at 830 cm−1 showed oxirane distribution in the sample (Figure 13). As blue and red colors indicate low and high oxirane content, respectively, the same trend was observed: an increase of nonreacted epoxy groups in the nanocomposite with unmodified silica nanoparticles. This may be explained based on matrix-nanoparticle interactions, where the incorporation of nanoparticles with high surface area in epoxy composite leads to intermolecular interactions between nanoparticles and polymer chains during curing process. As NanoSiO2-APTES nanoparticles present amine groups at the surfaces, covalent bonds between organofunctional group from surface modifier and epoxy groups from FBE can occur promoting the opening of oxirane rings. On the contrary, the epoxy-NanoSiO2 interaction may prevent the contact of dicyandiamide curing agent with the epoxy resin.Figure 12
Relative intensity of oxirane bands at (a) 830 cm−1 and (b) 3060 cm−1 of the FBE coatings.
(a) (b)Figure 13
FTIR-I image mapping of oxirane band at 830 cm−1 for (a) FBE, (b) FBE-NanoSiO2, and (c) FBE-NanoSiO2-APTES.
(a) (b) (c)In addition to FTIR analysis, the changes in glass transition temperature (Tg) of the cured epoxy coating (referred to as Tg2) can be used to characterize the degree of cure of the epoxy resin. In Figure 14, the results of Tg2 of coatings after the curing treatment obtained from DSC curves are displayed, where FBE-NanoSiO2-APTES nanocomposite presented higher Tg2 than FBE. As the epoxy curing process evolves, the degree of crosslinking increases, and the chains motion becomes more restricted. As a consequence, the glass transition temperature shifts to higher values, which can be readily observed by DSC analysis. These results are in agreement with FTIR analysis indicating that, besides the crosslinking promoted by reaction between DGEBA and DDA, the epoxy resin may have also reacted with the amino-modified nanofiller. The opposite effect was observed for FBE-NanoSiO2 revealing incomplete crosslinking in the presence of NanoSiO2 particles.Figure 14
(a) DSC curves for (A) FBE, (B) FBE-NanoSiO2, and (C) FBE-NanoSiO2-APTES. (b) Histogram of Tg2 values of the composite samples.
(a) (b)Figure15(a) shows the load displacement curves for the nanoindentation tests and Figure 15(b) the AFM image of a representative nanoindentation mark. Figures 15(c), 15(d), and 15(e) show the variation of the main parameters obtained by nanoindentation tests summarized in Table 2. The ratio between the elastic modulus (E) and the hardness (H) can be correlated with the fracture toughness of the coatings, if the geometry and the dimension of the nanoindentation marks are very similar [29–31]. The elastic modulus and hardness parameters measured for the original FBE are comparable to the reported values of epoxy coatings [32]. However, it can be observed that the addition of silica nanoparticles chemically modified by 3-APTES in FBE (FBE-NanoSiO2-APTES) significantly increased the elastic modulus and the hardness of coating by approximately 17%. Thus, these nanomaterials behaved as reinforcements in the epoxy-based matrix of FBE mostly due to the combination of two aspects: (a) the high superficial interfacial area between the epoxy resin and these nanoparticles and (b) the chemical reaction of epoxy oxirane groups with the amine-modified surfaces of the nanoparticles [33, 34]. The second aspect predominantly accounted for the higher effect as the nanosilica particles without chemical modification did not present detectable changes in these parameters compared to the original FBE system. Finally, the ratio E/H, which can be correlated with fracture toughness of the coatings, has not changed significantly among the samples. Therefore, the mechanical properties were increased by the incorporation of amino-modified silica nanoparticles in the FBE without affecting the toughness of nanocomposite, which is relevant as far as the application in high-performance coatings is concerned.Table 2
Elastic modulus (E), hardness (H), and the ratio (E/H) of the samples tested.
Sample E (GPa) H (MPa) E / H FBE 3.6 ± 1.0 282 ± 81 12.8 ± 0.6 FBE-NanoSiO2 3.5 ± 0.4 273 ± 47 12.7 ± 0.3 FBE-NanoSiO2-APTES 4.2 ± 1.0 331 ± 103 12.7 ± 0.6Figure 15
(a) Force-displacement curves of nanoindentation of FBE (A), FBE-NanoSiO2 (B), and FBE-NanoSiO2-APTES (C). (b) AFM image showing the nanoindentation mark produced. (c) Elastic modulus, (d) hardness, and (e) the ratio E/H for the three nanoindentation tests.
(a) (b) (c) (d) (e)Figure16 shows schematic drawings of the interactions between NanoSiO2-APTES and FBE that can enhance mechanical properties through the development of covalent bonds between the organofunctional group from surface modifier and the cured FBE.Figure 16
Schematic representation at the interface polymer-nanofiller in FBE-NanoSiO2-APTES nanocomposite.


## 3.1. Characterization of Bare Silica and Amino-Modified Silica Nanoparticles

The sol-gel process is widely used to produce pure silica particles due to the ability of this method to control the particle size, size distribution, and morphology through systematic monitoring of reaction parameters. The process involves hydrolysis and condensation of silicon alkoxides (Si(OR)4) such as tetraethylorthosilicate (TEOS, Si(OC2H5)4) in the presence of mineral acid (e.g., HCl) or base (e.g., NH3) as catalyst [1]. In this study, the sol-gel process was used to synthesize pure silica and organically modified silica nanoparticles for reinforcement of epoxy-based nanocomposite coatings. The TEM results presented in Figure 2 evidenced that the silica nanoparticles were spherical and reasonably monodispersed with the average size of 53±13 nm, where no significant effect of the amino functionalization was detected on the morphological aspects and size distribution of the silica nanoparticles. In addition, the EDX chemical analysis showed silicon and oxygen as the predominant elements, besides the other constituents of the grid (e.g., copper and carbon). These results demonstrated that nanosized silica particles were successfully produced with dimensions within controllable rangevia facile sol-gel processing route. It is worth highlighting that the properties of nanomaterials are usually size dependent, which can be designed and produced by controlling the size distribution of the nanoparticles by sol-gel processing route [1]. In addition, the amount of atoms residing on the surface increases with the decrease in particle size, which affects the amount of chemical groups present on the surface, such as silanols (-Si-OH) and incorporated functional species (i.e., -NH2). Thus, the combination of size and chemical functionalities of silica nanoparticles assigns unique properties to the resulting nanocomposites reinforced by these nanomaterials.Figure 2
(a) Typical TEM image of silica nanoparticles. (b) EDX spectrum of silica nanoparticles. (c) Nanoparticles size distribution.
(a) (b) (c)FTIR is used as a powerful tool for accessing the chemical groups during the synthesis after the formation of silica particles and the incorporation of functional groups at the surfaces. In Figure3 the FTIR spectra of bare silica (a) and amino-modified silica nanoparticles (b) are presented. As a general trend, in both spectra the major chemical species associated with the sol-gel synthesis of silica nanoparticles is clearly observed, such as stretching vibration bands of Si-O-Si at 1130–1000 cm−1 and 800 cm−1. In addition, the stretching vibration bands of Si-OH groups at 975–865 cm−1 related to the incomplete condensation of silanols. The broad band at 3700–3200 cm−1 is predominantly assigned to the stretching band of hydroxyl groups (Si-OH) in the structure and at the surface of the silica nanoparticles. In particular, the FTIR spectrum of Figure 3(b) shows the vibration bands at 3500–3200 cm−1 associated with amine groups (-NH2) overlapped with signals from silanol groups and the bands at 1573 cm−1 and 1318 cm−1 that are associated with the in-plane scissoring bending of primary amines (-NH2) and the stretching of -CN bonds, respectively [16]. Moreover, alkyl bands (-CH2) were detected at 2925, 2853 cm−1, and 1465 cm−1, which were assigned to the stretching and bending contributions of propyl groups present in the organosilane used as chemical surface modifier. Hence, these results evidenced that silica nanoparticles were produced by the sol-gel process with silanol groups incorporated in the structure. Furthermore, it was possible to chemically modify the system by adding amino-derived organosilane precursor during the sol-gel synthesis, leading to the formation of surface-functionalized silica nanoparticles. These aspects are relevant as far as the reinforcement of composite with nanoparticle is concerned because they offer chemical functional groups for the reaction with the epoxy-based matrix, which can potentially change the overall properties of the system.Figure 3
FTIR spectra of (a) silica nanoparticles and (b) silica nanoparticles modified by 3-APTES.Thermogravimetric (TG) analyses of the silica nanoparticles are presented in Figure4. TG analyses showed two-stage mass losses for bare silica nanoparticles (regions A and B) and three stages for amino-functionalized silica samples (3-APTES, regions A, B, and C) as shown in Figures 4(a) and 4(b), respectively. Essentially, for both silica samples (a and b) significant mass losses of approximately 8% below the temperature of 144°C (region A, Figure 4) can be observed, which were assigned to the drying process by evaporation and desorption of liquids, that is, ethanol and water molecules, from the silica nanoparticulate system [17]. The second stage of mass loss at region B of approximately 2%, between 144°C and 455°C (Figure 4), was predominantly associated with the release of trapped water within the pore, degradation of residual unreacted precursors (e.g., alcohol, ammonia, and silane), and dehydroxylation of silanol groups (Si-OH) and formation of siloxane linkage (Si-O-Si) [18]. Finally, from 455°C to 900°C (region C) silica nanoparticles presented just minor gradual mass loss (~1%) mostly due to the further increase in siloxane bonds and reduced surface hydroxyl groups. However, the amino-modified nanoparticles showed significant additional mass loss of approximately 4%, as a result of the degradation of the aminopropyl functional groups at the surface of the silica nanoparticles. The identification of these stages is very important because they evidenced the transition temperatures and range of temperature required for posterior thermal treatment of the silica nanoparticles after the synthesis in order to promote their densification and thermochemical stabilization. This aspect is crucial for utilizing these silica nanoparticles as inorganic reinforcements of epoxy-based nanocomposites. Therefore, based on the aforementioned reasons, this study used the heat thermal treatment of the silica nanoparticles (bare and amino-modified) at temperatures 200°C and 400°C in order to promote their thermochemical stabilization prior to the incorporation in the epoxy matrix. The curing temperature of the epoxy matrix was 248°C, much lower than the temperature of the thermally stabilized silica nanoparticles avoiding any degradation, which would negatively affect the properties of the FBE nanocomposite coatings.Figure 4
Thermal analyses of silica nanoparticles (a) before and (b) after surface functionalization with 3-APTES.The freshly synthesized silica nanoparticles (in gel form) were dried under heat drying in oven for 2 h. Drying process can cause the decrease in gel volume due to the loss of liquid by evaporation through the silica pore. Conversely, silica nanoparticles tend to coalesce upon heating, which can lead to changes in the average size of the nanoparticles. In this study, particle size distribution of the two silica nanoparticulate systems, with and without surface chemical functionalization, showed no significant statistical difference before and after the thermal treatment at 200°C and 400°C. SEM images (Figure5) reveal a relative narrow size distribution and sphere-like shape on the morphological aspects of the nanosize particles with roughly intermediate aggregation and agglomeration “as synthesized” (Figures 5(a) and 5(b)) due to presence of interparticle moisture. After heating at the temperatures of 200°C (Figures 5(c) and 5(d)) and 400°C (Figure 5(e)) a relative gradual reduction of agglomerates was observed, leading to more uniformly dispersed nanoparticulate systems mostly caused by the removal of water molecules upon heating and, therefore, decreasing the attractive hydrogen bonds between silica nanoparticles [19].Figure 5
SEM images of silica nanoparticles “as synthesized” ((a) nonfunctionalized and (b) amino-functionalized) and after heating at 200°C ((c) nonfunctionalized and (d) amino-functionalized) and at 400°C ((e) nonfunctionalized).
(a) (b) (c) (d) (e)The broad range of applications of silica nanoparticles is often determined by the particle size in the colloidal media. Dynamic light scattering (DLS) is a characterization technique that provides fast, accurate, and repeatable nanoparticle size data and therefore is an essential tool for the preliminary investigation of silica nanoparticles produced via sol-gel chemistry and the effect of surface functionalization by organic species. Hence, in this study, DLS measurements were performed in order to evaluate the hydrodynamic radius (HD) of the silica nanoparticles as synthesized and after surface functionalization with aminopropyl silane. The results are presented in Figure 6, with average HD values of 93±9 nm and 152±65 nm (polydispersity index = 0.032) for bare silica nanoparticles (Figure 6(a)) and APTES-silica nanoparticles (Figure 6(b)), respectively. These size distribution plots indicated the formation of reasonably monodispersed systems [20]. In addition, the results demonstrated the effective chemical functionalization of the silica nanoparticle surfaces, which caused the drastic increase in HD of the silica-modified nanoparticles (i.e., interparticle spacing), because of the steric hindrance and repulsion of hydrophobic aminopropyl groups at the surfaces dispersed in the hydrophilic water medium. The suggested schematic representation of the silica nanoparticles colloidal systems synthesized in this work is depicted in Figure 7.Figure 6
DLS measurements of silica nanoparticles (a) before and (b) after surface functionalization with 3-APTES. (c) Schematic representation of surface interaction of silica nanoparticles in water medium.Figure 7
Schematic representation of nanoparticle chemical structure: (a) NanoSiO2 and (b) NanoSiO2-APTES.
(a) (b)


## 3.2. Characterization of Epoxy-Based Nanocomposite Coating Reinforced with Silica and Surface-Modified Nanoparticles

Epoxy resin of Scotchkote 226N is based on poly(bisphenol A-co-epichlorohydrin) end-capped glycidyl (DGEBA, Figure8(a)) and dicyandiamide (DDA, Figure 8(b)) as the curing agent. Despite the complex mechanisms and reactions involved the curing process starts with the diffusion of the dicyandiamide into the epoxy resin and the reaction between the oxirane rings of DGEBA and primary and secondary amine groups of the dicyandiamide breaking the oxirane ring and forming hydroxyl groups. As the reaction proceeds, the formed hydroxyl groups can react with cyano and oxirane groups leading to the formation of a highly crosslinked network [21–23].Figure 8
Chemical structures of DGEBA (a) and DDA (b).
(a) (b)SEM images obtained from freeze-fractured surfaces of coatings are shown in Figure9. BSE image of FBE coating (Figure 9(a)) indicated that it is a composite material with inorganic particles (bright) immersed in the polymeric matrix (dark). As reported in a previous study developed by our group [9], the inorganic filler of FBE used in this study consists of small rounded anatase (TiO2) particles and needle-shaped wollastonite (CaSiO3) with different grades of aspect ratio in an amount of 32.8±3.0 wt.%. Morphologies of FBE nanocomposites reinforced with NanoSiO2 (Figure 9(b)) and NanoSiO2-APTES (Figure 9(c)) were similar to those observed for original FBE with only small agglomerates of silica nanoparticles (red arrows), with dimensions smaller than the calcium silicate nanoparticles. For all coating samples, secondary electron images of fractured materials (Figures 9(d), 9(e), and 9(f)) revealed a surface with distracted patterns that is characteristic of epoxy matrix reinforced with particles. This feature is usually associated with the presence of well bonded filler materials causing local yielding of the epoxy matrix, in contrast to the smooth and glassy brittle fracture surface of pure epoxy reported in literature [24]. In this sense, the addition of nanofiller into the FBE composite did not significantly affect the fracture surface, probably due to the small amount of SiO2 nanoparticles (1.0 wt.%) incorporated in epoxy-based material that already presented microsized inorganic fillers. The main difference between the FBE composite and the FBE nanocomposites (FBE-NanoSiO2 and FBE-NanoSiO2-APTES) is the presence of some voids in the epoxy matrix due to the presence of residual volatile components (Figures 9(e) and 9(f), white arrows) [18, 25]. Based on the thermal analysis of the silica nanoparticles presented in Section 3.1, there is still a loss of mass associated with the condensation of silanol groups, release of trapped water, and degradation of residual unreacted precursors at curing temperature (248±5°C) that is higher than the temperatures of 200°C used to stabilize and disperse the nanoparticles. On the other hand, no voids were observed for silica nanoparticles thermally treated at 400°C (Figure 10).Figure 9
Morphological analysis of FBE coatings (a and d) FBE, (b and e) FBE-NanoSiO2, and (c and f) FBE-NanoSiO2-APTES (red arrows, SiO2 agglomerates, and white arrows, voids).
(a) (b) (c) (d) (e) (f)Figure 10
Typical SE image of FBE-NanoSiO2-APTES coating with nanoparticles heat dried at 400°C.Based on precursors and main reactions involved in the formation of crosslinked epoxy matrix, midinfrared spectrum of cured FBE presented vibration bands associated with hydroxyl groups, oxirane ring, methyl and methylene groups, and C=C and C-C bonds in aromatic structures, besides ether and primary and secondary amines as depicted in Figure11 [21, 22, 26, 27]. In addition, bands associated with the silicate mineral filler (Si-O-Si and Si-O-Ca) were also observed.Figure 11
FTIR spectra of (A) FBE, (B) FBE-NanoSiO2, and (C) FBE-NanoSiO2-APTES in the spectral range of 4000–2500 cm−1 (a) and 1800–675 cm−1 (b).
(a) (b)To perform a deeper analysis, the curing extent of each system (FBE, FBE-NanoSiO2, and FBE-NanoSiO2-APTES) was evaluated by the absorbance intensity (A) of two bands related to oxirane ring (830 cm−1 and 3060 cm−1) normalized using the absorbance intensity of the C=C band of phenyl ring at 1608 cm−1 that is not involved in curing reactions remaining constant throughout [28]. The oxirane ring bands decrease upon curing as a consequence of the epoxy ring opening and, as the relative intensity of FTIR band is proportional to the relative concentration of the components in a sample, the results of the ratio ofA830/A1608 andA3060/A1608 (Figures 12(a) and 12(b), resp.) revealed that the FBE-NanoSiO2-APTES presented a higher degree of consumption of epoxy resin followed by FBE and FBE-NanoSiO2. FTIR image maps (FTIR-I analysis) of oxirane band at 830 cm−1 showed oxirane distribution in the sample (Figure 13). As blue and red colors indicate low and high oxirane content, respectively, the same trend was observed: an increase of nonreacted epoxy groups in the nanocomposite with unmodified silica nanoparticles. This may be explained based on matrix-nanoparticle interactions, where the incorporation of nanoparticles with high surface area in epoxy composite leads to intermolecular interactions between nanoparticles and polymer chains during curing process. As NanoSiO2-APTES nanoparticles present amine groups at the surfaces, covalent bonds between organofunctional group from surface modifier and epoxy groups from FBE can occur promoting the opening of oxirane rings. On the contrary, the epoxy-NanoSiO2 interaction may prevent the contact of dicyandiamide curing agent with the epoxy resin.Figure 12
Relative intensity of oxirane bands at (a) 830 cm−1 and (b) 3060 cm−1 of the FBE coatings.
(a) (b)Figure 13
FTIR-I image mapping of oxirane band at 830 cm−1 for (a) FBE, (b) FBE-NanoSiO2, and (c) FBE-NanoSiO2-APTES.
(a) (b) (c)In addition to FTIR analysis, the changes in glass transition temperature (Tg) of the cured epoxy coating (referred to as Tg2) can be used to characterize the degree of cure of the epoxy resin. In Figure 14, the results of Tg2 of coatings after the curing treatment obtained from DSC curves are displayed, where FBE-NanoSiO2-APTES nanocomposite presented higher Tg2 than FBE. As the epoxy curing process evolves, the degree of crosslinking increases, and the chains motion becomes more restricted. As a consequence, the glass transition temperature shifts to higher values, which can be readily observed by DSC analysis. These results are in agreement with FTIR analysis indicating that, besides the crosslinking promoted by reaction between DGEBA and DDA, the epoxy resin may have also reacted with the amino-modified nanofiller. The opposite effect was observed for FBE-NanoSiO2 revealing incomplete crosslinking in the presence of NanoSiO2 particles.Figure 14
(a) DSC curves for (A) FBE, (B) FBE-NanoSiO2, and (C) FBE-NanoSiO2-APTES. (b) Histogram of Tg2 values of the composite samples.
(a) (b)Figure15(a) shows the load displacement curves for the nanoindentation tests and Figure 15(b) the AFM image of a representative nanoindentation mark. Figures 15(c), 15(d), and 15(e) show the variation of the main parameters obtained by nanoindentation tests summarized in Table 2. The ratio between the elastic modulus (E) and the hardness (H) can be correlated with the fracture toughness of the coatings, if the geometry and the dimension of the nanoindentation marks are very similar [29–31]. The elastic modulus and hardness parameters measured for the original FBE are comparable to the reported values of epoxy coatings [32]. However, it can be observed that the addition of silica nanoparticles chemically modified by 3-APTES in FBE (FBE-NanoSiO2-APTES) significantly increased the elastic modulus and the hardness of coating by approximately 17%. Thus, these nanomaterials behaved as reinforcements in the epoxy-based matrix of FBE mostly due to the combination of two aspects: (a) the high superficial interfacial area between the epoxy resin and these nanoparticles and (b) the chemical reaction of epoxy oxirane groups with the amine-modified surfaces of the nanoparticles [33, 34]. The second aspect predominantly accounted for the higher effect as the nanosilica particles without chemical modification did not present detectable changes in these parameters compared to the original FBE system. Finally, the ratio E/H, which can be correlated with fracture toughness of the coatings, has not changed significantly among the samples. Therefore, the mechanical properties were increased by the incorporation of amino-modified silica nanoparticles in the FBE without affecting the toughness of nanocomposite, which is relevant as far as the application in high-performance coatings is concerned.Table 2
Elastic modulus (E), hardness (H), and the ratio (E/H) of the samples tested.
Sample E (GPa) H (MPa) E / H FBE 3.6 ± 1.0 282 ± 81 12.8 ± 0.6 FBE-NanoSiO2 3.5 ± 0.4 273 ± 47 12.7 ± 0.3 FBE-NanoSiO2-APTES 4.2 ± 1.0 331 ± 103 12.7 ± 0.6Figure 15
(a) Force-displacement curves of nanoindentation of FBE (A), FBE-NanoSiO2 (B), and FBE-NanoSiO2-APTES (C). (b) AFM image showing the nanoindentation mark produced. (c) Elastic modulus, (d) hardness, and (e) the ratio E/H for the three nanoindentation tests.
(a) (b) (c) (d) (e)Figure16 shows schematic drawings of the interactions between NanoSiO2-APTES and FBE that can enhance mechanical properties through the development of covalent bonds between the organofunctional group from surface modifier and the cured FBE.Figure 16
Schematic representation at the interface polymer-nanofiller in FBE-NanoSiO2-APTES nanocomposite.


## 4. Conclusion

In this work, nanosized silica particles were successfully produced using sol-gel process with organosilane precursors, characterized by the formation of monodisperse size distributions (53±13 nm) of bare silica and aminopropyl-modified silica nanoparticles (NanoSiO2 and NanoSiO2-APTES). The SEM and TEM analyses showed that these silica nanoparticles were uniformly dispersed in the FBE epoxy powder and, after the thermal treatment and curing process, led to the formation of homogenous nanocomposite coatings (nanoFBE). Midinfrared spectroscopy (MID) combined FTIR microscopy demonstrated that the amino-modified silica nanoparticles (NanoSiO2-APTES) reacted with the bisphenol-A diglycidyl ether from the FBE epoxy matrix, causing the formation of interfacial covalent bonds, which render more uniform nanocomposite coatings and increased the glass transition temperature compared to the FBE coating without nanoparticle reinforcement. These results were interpreted as the combination of several chemical reactions that may have occurred with the different chemical functional groups present in the system, besides the amino groups, such as silanol groups at the surface of the silica nanoparticles with the epoxy ring and with the dispersed inorganic components (calcium silicates and TiO2 particles) already present in the composition of FBE composite powder. These interfacial reactions between organic-inorganic and inorganic-inorganic components produced hybrid nanostructures in the novel composite matrix, which have improved the mechanical properties accessed by nanoindentation tests. Thus, it is expected that the superior integration between nanoparticles and epoxy matrix can enhance the overall performance of the system against underwater corrosion because of the improvement of mechanical properties. In summary, it is envisioned that these nanocomposite coatings can play a crucial role in the future to improve the mechanical properties of FBE systems and concurrently against highly corrosive environments for preventing catastrophic environmental accidents.



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*Source: 7281726-2016-10-27.xml*