# Triazole-ResistantAspergillus fumigatus from Fungicide-Experienced Soils in Naivasha Subcounty and Nairobi County, Kenya

Authors: Edson K. Kemoi; Andrew Nyerere; Christine C. Bii
Journal: International Journal of Microbiology
 (2018)

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## Abstract

The mainstay in prevention and treatment of aspergillosis is the use triazole drugs. In Kenya, the use of agricultural azole is one of the predisposing factors in development of resistance. One hundred fifty-six (156) experienced soils were collected from agricultural farms and cultured on Sabouraud DextroseAagar. The study isolated 48 yieldedAspergillus fumigatus and 2 A. flavus. All the isolates were subjected to antifungal susceptibility testing against three triazoles: posaconazole, voriconazole, and itraconazole. Out of the isolates, 3 had MIC of 32 and 1 had MIC of 16 against itraconazole, and 1 isolate had MIC of 32 against posaconazole. CYP51A gene was sequenced, and TR34/L98H mutation was identified. Triazole resistance existing in Kenya calls for rational use of azole-based fungicides in agriculture over concerns of emerging antifungal resistance in clinical practice.


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## Body

## 1. Introduction

Aspergillus species, especially Aspergillus fumigatus, is the most common cause of aspergillosis, which is the second leading cause of death after cryptococcosis in patients suffering from fungal infections [1]. Azole-resistant Aspergillus fumigatus is an evolving global health challenge [2]. It is a frequent colonizer of cavitary lesions in tuberculosis patients and cause of mortality in post-TB treatment cases [3].Aspergillosis treatment is done by using amphotericin B or azoles. However, resistance against azoles has been increasingly reported especially from high- and middle-income countries [4–12]. Previous studies from different regions of the world—African region (Tanzania), European region (Netherlands, Belgium, Denmark, and Germany), Asia (Kuwait, India, and Iran), and the USA—have reported multiple sources of azole-resistant Aspergillus fumigatus from soil sample, flower beds, plants, compost, and hospitals and its environs [4, 10, 12–23].In contrast, limited data are available from low-income countries, especially from sub-Saharan Africa. However, a recent disturbing report of high resistance to azole was reported in Moshi, Tanzania associated withA. fumigatus with TR34/L98H and TR46/Y12F/T289A mutations [20]. The widespread irrational use of azole-based agricultural fungicides in the flower and horticultural industry in Kenya is a significant risk factor for azole resistance. The study aimed to determine the prevalence of triazole resistance among Aspergillus fumigatus from fungicide-experienced soils.


## 2. Methodology

### 2.1. Study Area

The study was conducted in Nairobi and Naivasha subcounty where horticultural practices and green houses are concentrated. Nairobi is the capital city of Kenya and lies at about 1°17′S and 36°49′E while Naivasha is located approximately 90 km northwest of Nairobi. It is located in Nakuru County at 0°43′S36°26E, and horticulture is the main economy. The trade names of commonly used fungicides include milraz, antracol, mistress, and victory which are broad-spectrum fungicides of ornamental, vegetable, and fruit plants (Figure1).Figure 1
A map showing the study areas. Courtesy ofwww.mapsofworld.com (accessed 12 January 2015).


### 2.2. Sampling for Environmental Isolates

A total of 156 samples were collected and analyzed. Approximately 5 g dry top surface soil from the agricultural site was collected into a sterile 15 ml Falcon tube using a sterile plastic spoon [23]. Samples were transported in a leak-proof packaging in a cool box to the Mycology Laboratory-KEMRI-Center for Microbiology Research for investigation. Clinical isolates were isolated from sputum samples of suspected aspergillosis patients and were archived at Kenya Medical Research Institute, Mycology Laboratory.


### 2.3. Fungal Culture and Identification

One gram of soil sample was mixed with 5 ml saponin, vortexed, and the debris was allowed to settle. One hundred microliters of the supernatant was transferred to 500µl of sterile normal saline and vortexed. Approximately 100 µl of the suspension was cultured onto Sabouraud dextrose agar containing (a). 0.001 mg/l of itraconazole, (b) 0.001 mg/l of voriconazole, and (c) without drug (control). All the inoculated plates were incubated for 5 days at 30°C [24].


### 2.4. Broth Dilution Sensitivity Testing

Aspergillus fumigatus growing on azole-supplemented media were subjected to antifungal susceptibility testing against three triazoles: posaconazole (PCZ), voriconazole (VCZ), and itraconazole (ITZ) using the CLSI M38-A2 broth microdilution method [25].


### 2.5. Sequencing of CYP51A Gene

Aspergillus fumigatus showing high MIC against itraconazole, voriconazole, and posaconazole had their CYP51A gene sequenced for detection of mutation as previously described [26].


## 2.1. Study Area

The study was conducted in Nairobi and Naivasha subcounty where horticultural practices and green houses are concentrated. Nairobi is the capital city of Kenya and lies at about 1°17′S and 36°49′E while Naivasha is located approximately 90 km northwest of Nairobi. It is located in Nakuru County at 0°43′S36°26E, and horticulture is the main economy. The trade names of commonly used fungicides include milraz, antracol, mistress, and victory which are broad-spectrum fungicides of ornamental, vegetable, and fruit plants (Figure1).Figure 1
A map showing the study areas. Courtesy ofwww.mapsofworld.com (accessed 12 January 2015).


## 2.2. Sampling for Environmental Isolates

A total of 156 samples were collected and analyzed. Approximately 5 g dry top surface soil from the agricultural site was collected into a sterile 15 ml Falcon tube using a sterile plastic spoon [23]. Samples were transported in a leak-proof packaging in a cool box to the Mycology Laboratory-KEMRI-Center for Microbiology Research for investigation. Clinical isolates were isolated from sputum samples of suspected aspergillosis patients and were archived at Kenya Medical Research Institute, Mycology Laboratory.


## 2.3. Fungal Culture and Identification

One gram of soil sample was mixed with 5 ml saponin, vortexed, and the debris was allowed to settle. One hundred microliters of the supernatant was transferred to 500µl of sterile normal saline and vortexed. Approximately 100 µl of the suspension was cultured onto Sabouraud dextrose agar containing (a). 0.001 mg/l of itraconazole, (b) 0.001 mg/l of voriconazole, and (c) without drug (control). All the inoculated plates were incubated for 5 days at 30°C [24].


## 2.4. Broth Dilution Sensitivity Testing

Aspergillus fumigatus growing on azole-supplemented media were subjected to antifungal susceptibility testing against three triazoles: posaconazole (PCZ), voriconazole (VCZ), and itraconazole (ITZ) using the CLSI M38-A2 broth microdilution method [25].


## 2.5. Sequencing of CYP51A Gene

Aspergillus fumigatus showing high MIC against itraconazole, voriconazole, and posaconazole had their CYP51A gene sequenced for detection of mutation as previously described [26].


## 3. Results

A total 156 fungicide-exposed soil samples were analyzed, out of which 48 yieldedAspergillus fumigatus and 2 A. flavus. Antifungal susceptibility testing against three triazoles, posaconazole, voriconazole, and itraconazole, indicates that 3 isolates had MIC = 32 µg/ml and other 2 had MIC = 4 µg/ml against itraconazole, 5 isolates had MIC = 8 µg/ml and 1 isolate had MIC = 16 µg/ml against voriconazole, and 1 isolate had MIC of 32 µg/ml and one had MIC = 16 µg/ml against posaconazole (Table 1). Two archived clinical Aspergillus fumigatus from KEMRI-Mycology were used for comparison (Table 1).Table 1
Sources of isolates and MIC against the three triazoles.
Number
Sources
Isolates
MIC against itraconazole
MIC against voriconazole
MIC against posaconazole
F1
Experienced soils
Aspergillus fumigatus
1
0.25
0.25
F2
Experienced soils
Aspergillus fumigatus
1
0.15
0.25
F3
Experienced soils
Aspergillus fumigatus
0.5
0.5
0.06
F4
Experienced soils
Aspergillus fumigatus
1
0.25
0.25
F5
Experienced soils
Aspergillus fumigatus
1
0.15
0.25
F6
Experienced soils
Aspergillus fumigatus
4
1
1
F7
Experienced soils
Aspergillus fumigatus
4
1
1
F8
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.25
F9
Experienced soils
Aspergillus fumigatus
0.5
0.06
0.15
F10
Experienced soils
Aspergillus fumigatus
1
0.5
0.25
F11
Experienced soils
Aspergillus fumigatus
0.5
0.13
0.06
F12
Experienced soils
Aspergillus fumigatus
0.5
0.06
0.06
F13
Experienced soils
Aspergillus fumigatus
0.5
0.03
0.25
F14
Experienced soils
Aspergillus fumigatus
0.25
0.15
0.06
F15
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.25
F16
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.15
F17
Experienced soils
Aspergillus fumigatus
0.13
0.5
0.25
F18
Experienced soils
Aspergillus fumigatus
1
1
1
F19
Experienced soils
Aspergillus fumigatus
0.15
0.06
0.25
F20
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.25
F21
Experienced soils
Aspergillus fumigatus
1
1
1
F22
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.5
F23
Experienced soils
Aspergillus fumigatus
32
8
0.5
F24
Experienced soils
Aspergillus fumigatus
1
0.25
0.25
F25
Experienced soils
Aspergillus fumigatus
1
8
0.5
F26
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.15
F27
Experienced soils
Aspergillus fumigatus
0.5
0.03
0.06
F28
Experienced soils
Aspergillus fumigatus
0.5
1
0.15
F29
Experienced soils
Aspergillus fumigatus
1
0.15
0.25
F30
Experienced soils
Aspergillus fumigatus
0.5
0.05
0.15
F31
Experienced soils
Aspergillus fumigatus
1
0.15
0.25
F32
Experienced soils
Aspergillus fumigatus
1
0.15
0.15
F33
Experienced soils
Aspergillus fumigatus
0.5
0.25
0.5
F34
Experienced soils
Aspergillus fumigatus
1
0.15
0.25
F35
Experienced soils
Aspergillus fumigatus
1
2
1
F36
Experienced soils
Aspergillus fumigatus
32
16
1
F37
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.25
F38
Experienced soils
Aspergillus fumigatus
0.5
0.25
0.15
F39
Experienced soils
Aspergillus fumigatus
1
0.25
0.25
F40
Experienced soils
Aspergillus fumigatus
32
8
32
F41
Experienced soils
Aspergillus fumigatus
1
0.15
0.15
F42
Experienced soils
Aspergillus fumigatus
0.5
0.15
0.15
F43
Experienced soils
Aspergillus fumigatus
0.5
0.06
0.06
F44
Experienced soils
Aspergillus fumigatus
8
8
16
F45
Experienced soils
Aspergillus fumigatus
1
8
0.5
F46
Experienced soils
Aspergillus fumigatus
0.25
0.006
0.25
F47
Experienced soils
Aspergillus fumigatus
0.06
0.25
0.25
F48
Experienced soils
Aspergillus fumigatus
0.5
0.13
0.5
C21
Clinical
Aspergillus fumigatus
4
4
2
C40
Clinical
Aspergillus fumigatus
2
1
0.13Triazole resistance levels of all of theAspergillus fumigatus isolates are summarized in Table 2. According to Arendrup et al. [27] breakpoints, the percentage of resistance against both itraconazole and voriconazole was 12.5% and susceptible cases at 87.5% and 85.4% against itraconazole and voriconazole, respectively. Against posaconazole, 27.1% were resistant, 60.4% were intermediates, and 12.5% were susceptible.Table 2
Triazole resistance levels of the isolatedAspergillus fumigatus.
Resistance
Itraconazole (n (%))
Voriconazole (n (%))
Posaconazole (n (%))
Resistant
6 (12.5)
6 (12.5)
13 (27.1)
Intermediates
0
1 (2.08)
29 (60.4)
Susceptible
42 (87.5)
41 (85.4)
6 (12.5)
Total
48 (100)
48 (100)
48 (100)Three samples with high MIC were subjected to sequence analysis for the detection of mutation in CYP51A in which TR34/L98H was confirmed (Table3).Table 3
Sequencing analysis of CYP51A of selectedAspergillus fumigatus.
Number
Mutation
STRS
Sources
F36
A. fumigatus
TR34/L98H
14
21
8
32
9
38
8
10
18
Environmental
F40
A. fumigatus
TR34/L98H
14
21
8
28
9
6
8
10
18
Environmental
C21
A. fumigatus
TR34/L98H
14
21
8
28
9
6
8
10
18
Clinical


## 4. Discussion

In our study, we report the presence of triazole-resistantAspergillus fumigatus from clinical- and fungicide-experienced soils collected from Naivasha and Nairobi, Kenya. Azole-resistant Aspergillus spp. have been detected worldwide including Asia, Europe, Middle East, Tanzania [20], and Kenya. Kemoi et al. reported prevalence of azole-resistant Aspergillus fumigatus between 19.23% and 36% from both naive and experience soils. However, the study did not sequence CYP51A gene to determine the type of mutations involved [21]. The finding of this study is of great medical implications especially in African environments where resources are limited and effective treatment and early diagnosis are a challenge. Posaconazole, itraconazole, and voriconazole are the first-line drugs used in management and prevention of aspergillosis; hence, detection of environmental isolates resistant to these triazoles poses great challenge in the medical field [27, 28].In Kenya, Naivasha subcounty is known for extensive flower farming with extensive use of fungicides. The use of azole-based fungicide in agriculture introduces antifungal pressure resulting in reduced susceptible fungi and increases azole-resistant strains [29]. Detection of A. fumigatus with TR34/L98H mutation is the first report in Kenya. It has been reported by several authors that isolates with mutations in TR34/L98H region have cross resistance to both medical azole and azole-based fungicides, for example, propiconazole, tebuconazole, difenoconazole, bromuconazole, and epoxiconazole [4, 12]. The TR34/L98H mutation in gene CYP51A involves the substitution of leucine 98 for histidine L98H and two 34-bp tandem copies in CYP51A gene in the promoter region [10, 30–33].Azole-based fungicides in the environment have been linked to TR/L98H mutation inAspergillus fumigatus. This form of resistance has been linked to point alteration in 220, 138, and 54 codons from patient on azole treatment [34]. Plant pathogenic molds having tandem repeat which aid in resistance against sterol demethylation inhibitor fungicides have been reported [35]. Because fungi including A. fumigatus and other plant fungal pathogens share the same habitat, they are constantly exposed to fungicide pressure. Therefore, if Aspergillus species harboring TR/L98H resistance is present in the environment, the conidia can be widely dispersed by wind and may cause infection in the susceptible individual [9].In Moshi, Tanzania, Azole-resistantAspergillus fumigatus was isolated from soil known for extensive farming. It was reported that 20% of the environmental samples harbor azole-resistant Aspergillus fumigatus, of which 5.5% was associated with TR46/Y122FT289A mutation and 20% TR34/L98H mutation was isolated from woody debris and soil samples [20]. The isolation of Aspergillus fumigatus with G54E mutation in Tanzania, Romania, and India from environmental samples is considered to occur in patients with prolonged exposure to azole [36]. The G54E mechanisms are responsible for 20.0% from India, 30.4% from Romania, and 46.4% of resistant isolates from Tanzania [36].


## 5. Conclusion

There is significant triazole resistance among environmental isolates ofAspergillus probably ascribed to irrational use of fungicide in agriculture and calls for legislative mechanism for control of fungicide use. This is a grave public health concern given the limited resources and the limited antifungal options available for the susceptible patients.



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*Source: 7147938-2018-06-26.xml*