# High CXCR3 on Leukemic Cells DistinguishesIgHVmut from IgHVunmut in Chronic Lymphocytic Leukemia: Evidence from CD5high and CD5low Clones

Authors: Gayane Manukyan; Tomas Papajik; Zuzana Mikulkova; Renata Urbanova; Veronika Smotkova Kraiczova; Jakub Savara; Milos Kudelka; Peter Turcsanyi; Eva Kriegova
Journal: Journal of Immunology Research
 (2020)

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## Abstract

Despite the shared pattern of surface antigens, neoplastic cells in chronic lymphocytic leukemia (CLL) are highly heterogeneous in CD5 expression, a marker linked to a proliferative pool of neoplastic cells. To further characterize CD5high and CD5low neoplastic cells, we assessed the chemokine receptors (CCR5, CCR7, CCR10, CXCR3, CXCR4, CXCR5) and adhesion molecules (CD54, CD62L, CD49d) on the CD5high and CD5low subpopulations, defined by CD5/CD19 coexpression, in peripheral blood of CLL patients (n=60) subgrouped according to the IgHV mutational status (IgHVmut, n=24; IgHVunmut, n=36). CD5high subpopulation showed a high percentage of CXCR3 (P<0.001), CCR10 (P=0.001), and CD62L (P=0.031) and high levels of CXCR5 (P=0.005), CCR7 (P=0.013) compared to CD5low cells expressing high CXCR4 (P<0.001). Comparing IgHVmut and IgHVunmut patients, high levels of CXCR3 on CD5high and CD5low subpopulations were detected in the IgHVmut patients, with better discrimination in CD5low subpopulation. Levels of CXCR3 on CD5low subpopulation were associated with time to the next treatment, thus further confirming its prognostic value. Taken together, our analysis revealed higher CXCR3 expression on both CD5high and CD5low neoplastic cells in IgHVmut with a better prognosis compared to IgHVunmut patients. Contribution of CXCR3 to CLL pathophysiology and its suitability for prognostication and therapeutic exploitation deserves future investigations.


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## Body

## 1. Introduction

Chronic lymphocytic leukemia (CLL) is a lymphoproliferative malignancy of clonally expanded heterogeneous pool of neoplastic B cells with aberrant expression of CD5 [1, 2], which are highly variably distributed between bone marrow, lymphoid organs, and peripheral blood. There is a growing body of evidence that proliferation of neoplastic cells plays a critical role in CLL pathogenesis [3–5], with the highest degree of proliferation being observed in the lymph nodes [6].CD5, a marker normally present on T cells, acts as a repressor of B-cell receptor (BCR) signalling in CLL [7]. Proliferating, migrating CLL cells have been shown to preferentially express high levels of CD5, while the low levels of CD5 are associated with resting, circulating cells [8]. The overlapping BCR repertoires between CD5high and CD5low cells suggest a dynamic relationship of these two B-CLL cell subpopulations [2]. The inversed expression of CD5 and CXCR4 was used for the identification of fractions enriched in recently born/divided and older/quiescent CLL cells [8, 9]. It has been shown that CXCR4dimCD5bright “proliferative” cells overexpress more “cell division” genes, while CXCR4brightCD5dim “resting” cells express higher levels of “antiproliferative” genes, suggesting that the last subset could be a distinct self-renewing one from which all clonal members derive [8, 9].Given the dynamic variability and heterogeneity of CD5 expression and its link to the proliferative pool of neoplastic cells, we aimed to further characterize the chemokine and adhesion molecule profile of CD5high and CD5low neoplastic clones using the novel CD5/CD19 model in peripheral blood of CLL patients. Being important for lymphocyte migration, we evaluated the expression of molecules linked to adhesion and extravasation (CD54, CD62L, CD49d), migration into lymph nodes (CCR7, CXCR3, CXCR5), homing lymphocytes to the bone marrow (CXCR4), and lymphocyte trafficking (CCR5, CCR10) in CD5high and CD5low neoplastic clones. Moreover, the differential expression pattern of chemokines and adhesion molecules on CD5high and CD5low clones was for the first time studied in two biologically and clinically distinct CLL subtypes defined by the abundance of somatic hypermutations affecting the Ig variable heavy-chain locus (IgHV), which markedly differ in their prognosis and response to the chemoimmunotherapy [10–12].


## 2. Materials and Methods

### 2.1. Patients

The patient cohort consisted of 60 patients with CLL, all diagnosed according to the IWCLL guidelines [13]. Patient subgroups were formed based on IgHV mutational status (IgHVmut, n=24; IgHVunmut, n=36). Clinical characteristics of CLL patients are shown in Table 1. All patients provided written informed consent for the use of peripheral blood for research purposes in accordance with the Declaration of Helsinki. The study was approved by the ethics committee of University Hospital and Palacký University Olomouc.Table 1
Patient demographic and clinical characteristics.
ParameterCLL (n=60)Age, years, median (min-max)67 (50-86)Gender (male/female)35/25Binet stage (A/B/C)26/23/11IgHV gene mutational status∗ (mutated/unmutated)24/36Genetics11q-/17p-13/6N/O6/46Not determined2Follow-up time months (mean, min–max)53 (0-160)Treatment history (yes/no)29/31Time of last treatment in treated patients (in respect to the sampling time) months (mean, min–max)23 (1-61)Time to the next treatment (in respect to the sampling time) months (mean, min–max)29 (0-49)CLL cells in peripheral bloodPercentage, mean (95% CI)69.3 (62.3-76.3)Absolute number (×109/L), mean (95% CI)49.1 (32.3-65.9)∗IgHV mutational status was defined as follows: IgHVunmut with a cut-off of 2% deviation or >98% sequence identity to germline in the IgHV sequence (13). 11q- and 17p-: any FISH or karyotypic abnormality involving 11q or 17p; N: no detectable cytogenetic aberration by FISH; O: other cytogenetic abnormality (excluding 11q- or 17p-).


### 2.2. Flow Cytometry Analysis of Chemokine Receptors and Adhesion Molecules

Cells in whole blood were stained with optimal concentrations of monoclonal antibody combinations directed against the following surface antigens: CD45-PerCP/Cy5.5, CD5-PE, CD19-APC/Cy7, CD54-FITC, CD62L-APC, CD49d-PE/Cy7, CD183-FITC (CXCR3), CD184-APC (CXCR4), CD185-FITC (CXCR5), CD197-PE/Cy7 (CCR7), CD195-PE/Cy7 (CCR5), CCR10-APC (all BioLegend), as reported previously [14, 15]. Isotype-matched antibodies were used as negative controls. The analysis was performed on BD FACSCanto II (Becton Dickinson). Data acquisition was performed using BD FACSDiva software (Becton Dickinson). Flow cytometry data were analysed using the FlowJo vX0.7 software (Tree Star, Inc, San Carlos, CA). In all experiments, a minimum of 10,000 events was counted. Results are expressed as the percentage and median fluorescence intensity (MFI).


### 2.3. Identification of CD5high and CD5low Subpopulations

Coexpression of CD5 and CD19, surface molecules essential for phenotypic characteristic of CLL cells, was used to discriminate between CD5high and CD5low subsets of CLL cells. Gating strategy for detection of CD5high and CD5low subpopulations and representative examples of CXCR3 and CXCR4 expression in IgHVmut and IgHVunmut patients are shown in Figure 1.Figure 1
(a) Gating strategy for detection of CD5high and CD5low subpopulations within CD5+CD19+neoplastic cells and visualization of their CXCR3 and CXCR4 expression. (b) CXCR3 and CXCR4 expression on CD5high and CD5low subpopulations in IgHVunmut and IgHVmut patient—representative example.
(a)(b)


### 2.4. Chemotaxis Assay

Peripheral blood mononuclear cells (PBMC) were isolated using a Ficoll density gradient centrifugation; only samples containing more than 70% CLL cells in PBMC were chosen for the assay. Transmigration of CLL cells was assessed using polycarbonate Transwell inserts with 5-μm pore size (Corning Costar). Briefly, the cells at 1×10exp6/mL were applied to the upper chamber in RPMI-1640 containing 1% bovine serum albumin (BSA) in the presence or absence of CXCL11 (BioLegend). Filters were transferred into the lower wells containing RPMI-1640 with 1% BSA in the presence or absence of CXCL12 (BioLegend). After 3 hours at 37°C in 5% CO2, cells that migrated into the lower chambers were counted and analysed for CXCR3 and CXCR4 expression on BD FACSCanto II.


### 2.5. Statistical and Data Mining Analyses

Statistical analyses (Mann-WhitneyU-test, paired Wilcoxon nonparametric test, Kruskal-Wallis test, 95% confidence intervals (CI), receiver operating characteristic (ROC) curves, the Kaplan-Meier curve, Spearman correlation analysis) were performed using the R statistical software package (http://www.r-project.org/). The multivariate patient similarity networks (PSNs) based on the nearest neighbour analysis [16, 17] were applied for the visualization of patient similarities of chemokine profiles. Correlation networks using LRNet algorithm [16] and Spearman’s rank correlation coefficient were constructed and visualized to investigate the relationships between expression of individual molecules on CLL cells [18]. P values <0.05 were considered significant.


## 2.1. Patients

The patient cohort consisted of 60 patients with CLL, all diagnosed according to the IWCLL guidelines [13]. Patient subgroups were formed based on IgHV mutational status (IgHVmut, n=24; IgHVunmut, n=36). Clinical characteristics of CLL patients are shown in Table 1. All patients provided written informed consent for the use of peripheral blood for research purposes in accordance with the Declaration of Helsinki. The study was approved by the ethics committee of University Hospital and Palacký University Olomouc.Table 1
Patient demographic and clinical characteristics.
ParameterCLL (n=60)Age, years, median (min-max)67 (50-86)Gender (male/female)35/25Binet stage (A/B/C)26/23/11IgHV gene mutational status∗ (mutated/unmutated)24/36Genetics11q-/17p-13/6N/O6/46Not determined2Follow-up time months (mean, min–max)53 (0-160)Treatment history (yes/no)29/31Time of last treatment in treated patients (in respect to the sampling time) months (mean, min–max)23 (1-61)Time to the next treatment (in respect to the sampling time) months (mean, min–max)29 (0-49)CLL cells in peripheral bloodPercentage, mean (95% CI)69.3 (62.3-76.3)Absolute number (×109/L), mean (95% CI)49.1 (32.3-65.9)∗IgHV mutational status was defined as follows: IgHVunmut with a cut-off of 2% deviation or >98% sequence identity to germline in the IgHV sequence (13). 11q- and 17p-: any FISH or karyotypic abnormality involving 11q or 17p; N: no detectable cytogenetic aberration by FISH; O: other cytogenetic abnormality (excluding 11q- or 17p-).


## 2.2. Flow Cytometry Analysis of Chemokine Receptors and Adhesion Molecules

Cells in whole blood were stained with optimal concentrations of monoclonal antibody combinations directed against the following surface antigens: CD45-PerCP/Cy5.5, CD5-PE, CD19-APC/Cy7, CD54-FITC, CD62L-APC, CD49d-PE/Cy7, CD183-FITC (CXCR3), CD184-APC (CXCR4), CD185-FITC (CXCR5), CD197-PE/Cy7 (CCR7), CD195-PE/Cy7 (CCR5), CCR10-APC (all BioLegend), as reported previously [14, 15]. Isotype-matched antibodies were used as negative controls. The analysis was performed on BD FACSCanto II (Becton Dickinson). Data acquisition was performed using BD FACSDiva software (Becton Dickinson). Flow cytometry data were analysed using the FlowJo vX0.7 software (Tree Star, Inc, San Carlos, CA). In all experiments, a minimum of 10,000 events was counted. Results are expressed as the percentage and median fluorescence intensity (MFI).


## 2.3. Identification of CD5high and CD5low Subpopulations

Coexpression of CD5 and CD19, surface molecules essential for phenotypic characteristic of CLL cells, was used to discriminate between CD5high and CD5low subsets of CLL cells. Gating strategy for detection of CD5high and CD5low subpopulations and representative examples of CXCR3 and CXCR4 expression in IgHVmut and IgHVunmut patients are shown in Figure 1.Figure 1
(a) Gating strategy for detection of CD5high and CD5low subpopulations within CD5+CD19+neoplastic cells and visualization of their CXCR3 and CXCR4 expression. (b) CXCR3 and CXCR4 expression on CD5high and CD5low subpopulations in IgHVunmut and IgHVmut patient—representative example.
(a)(b)


## 2.4. Chemotaxis Assay

Peripheral blood mononuclear cells (PBMC) were isolated using a Ficoll density gradient centrifugation; only samples containing more than 70% CLL cells in PBMC were chosen for the assay. Transmigration of CLL cells was assessed using polycarbonate Transwell inserts with 5-μm pore size (Corning Costar). Briefly, the cells at 1×10exp6/mL were applied to the upper chamber in RPMI-1640 containing 1% bovine serum albumin (BSA) in the presence or absence of CXCL11 (BioLegend). Filters were transferred into the lower wells containing RPMI-1640 with 1% BSA in the presence or absence of CXCL12 (BioLegend). After 3 hours at 37°C in 5% CO2, cells that migrated into the lower chambers were counted and analysed for CXCR3 and CXCR4 expression on BD FACSCanto II.


## 2.5. Statistical and Data Mining Analyses

Statistical analyses (Mann-WhitneyU-test, paired Wilcoxon nonparametric test, Kruskal-Wallis test, 95% confidence intervals (CI), receiver operating characteristic (ROC) curves, the Kaplan-Meier curve, Spearman correlation analysis) were performed using the R statistical software package (http://www.r-project.org/). The multivariate patient similarity networks (PSNs) based on the nearest neighbour analysis [16, 17] were applied for the visualization of patient similarities of chemokine profiles. Correlation networks using LRNet algorithm [16] and Spearman’s rank correlation coefficient were constructed and visualized to investigate the relationships between expression of individual molecules on CLL cells [18]. P values <0.05 were considered significant.


## 3. Results

### 3.1. CD5/CD19 Markers as Identifiers of CD5high and CD5low Cells

First, we identified CD5high and CD5low cells based on the CD5/CD19 model and compared it with the reported CD5low/CXCR4high model [8, 9]. The CD5high cells in the CD5/CD19 model corresponded to those in the CD5high/CXCR4low model, and similarly CD5low cells corresponded to those in the CD5low/CXCR4high one (Figure S1A). High interindividual variability in the proportion of both closely related subpopulations of CD5high and CD5low cells was observed, irrespective of IgHV mutational status and other clinical characteristics (Figure S1B).


### 3.2. Differential Expression of Chemokine Receptors and Adhesion Molecules on CD5high and CD5low CLL Cells

The expression of CD54, CD62L, CD49d, CCR5, CCR7, CCR10, CXCR3, CXCR4, and CXCR5 was evaluated on CD5high and CD5low subpopulations (Table S1).When analysing levels of studied markers (MFI) in CD5high and CD5low subpopulations, the CD5high cells expressed higher levels of CXCR5 (P=0.005) and CCR7 (P=0.013) and lower levels of CXCR4 receptor (P<0.001) than the population of CD5low cells. Besides, more CD5high cells were positive for CXCR3 (P<0.001), CCR5 (P=0.012), CCR10 (P=0.007), and CD62L (P=0.047) than CD5low cells (Figure 2, Table S1).Figure 2
Distribution of (a) percentages of positive cells for CXCR3, CCR10, and CD62L and (b) expression (MFI) of CXCR4, CXCR5, and CCR7 on CD5high and CD5low subpopulations in patients with CLL. Group means are indicated by horizontal bars, error bars indicate 95% CI.
(a)(b)


### 3.3. Characterization of CD5high and CD5low CLL Cells in Patient Subgroups according to the IgHV Mutational Status

To characterize the CLL cells and their subpopulations in patient subgroups according to theIgHV mutational status, we compared the expression of studied markers on CLL cells as a whole and separately on CD5high and CD5low cells in CLL patients with IgHVmut and IgHVunmut status (Figure 3, Table S2, Table S3).Figure 3
Distribution of CXCR3 and CXCR4 chemokines inIgHVmut (violet) and IgHVunmut (red) patients in (a) CLL cells as a whole, (b) CD5high, and CD5low subpopulations in a paired analysis. The lines connect the values on CD5high and CD5low subpopulations in the same patient.
(a)(b)Of the studied markers, patients withIgHVmut had a higher percentage of cells expressing CXCR3 (P=0.003, Figure 3(a)) and CD62L (P=0.003) in the whole population of CLL cells compared to those with IgHVunmut status. When IgHVmut and IgHVunmut patients were evaluated separately, in both groups, a higher proportion of CLL cells positive on CXCR3 (in both P<0.001) and lower expression of CXCR4 (in both P<0.001) was observed on CD5high subpopulation in comparison with CD5low cells (Figure 3(b)). Similarly, when CD5high and CD5low cell populations were evaluated separately, the IgHVmut group exhibited higher percentages of CXCR3 (in both P<0.001), CD62L (in both P=0.003) positive cells, as well as higher expression of CXCR5 (P<0.001 and P=0.011, respectively) in comparison with IgHVunmut (Table S2, Table S3).To exclude possible influence of the treatment on the studied parameters, we performed subanalysis on a cohort of untreated patients subdivided according to theIgHV mutational status, and we confirmed the differences for studied markers observed in the whole patient cohort (Figure S2A). Moreover, we did not observe significant differences in studied markers between IgHVunmut patients untreated and those with treatment history (Figure S2B).


### 3.4. Correlation of CXCR3, CXCR4, and CXCR5 with CD5 Expression on CLL Cells

On CLL cells, CD5 expression (MFI) positively correlated with the percentages and MFI of CXCR3 (rs=0.54, P<0.001 and rs=0.54, P<0.001) and MFI of CXCR5 (rs=0.34, P=0.010). There was a trend towards inverse correlation between CD5 and CXCR4 expression on the whole CLL subpopulation (rs=−0.23, P=0.086) (Figure 4(a)). Further information about correlations of CXCR3, CXCR4, and CXCR5 expression on CD5high and CD5low subpopulations is provided in the Supplementary file.Figure 4
Correlation of CD5 expression with (a) percentages of CXCR3, levels (MFI) of CXCR4 and CXCR5 on CLL cells, and (b) studied chemokines on CLL cells assessed by the correlation network analysis.
(a)(b)Regarding the relationship between CXCR3 and CXCR4, correlation analysis revealed a negative correlation between percentages as well as expression (MFI) of CXCR3 with CXCR4 on CLL cells (rs=−0.35, P=0.009 and rs=−0.38, P=0.006, respectively) (Figure S3). Network correlation analysis among studied chemokines and CD5 further confirmed a relationship and importance of CD5-CXCR3-CXCR5 axis on CLL cells (Figure 4(b)).


### 3.5. Migration Rate of CLL Cells in the Presence of CXCL11

To analyse the cooperative interplay between CXCR3 and CXCR4, we analysed the migratory ability of CXCL11-treated and untreated CLL cells towards chemokine CXCL12. The highest migration rates were observed for the CXCL11-untreated cells that migrated towards CXCL12 (P=0.010) (Figure 5). When CLL cells were treated with CXCL11, their spontaneous migration as well as migration rate toward CXCL12 decreased (Figure 5).Figure 5
Cooperative effect of CXCL11 and CXCL12 on the migratory ability of CLL cells. CLL cells (n=6), with/without pretreatment with 200 ng/mL CXCL11, were added to the upper chamber of Transwell inserts. 200 ng/mL CXCL12 was added to the medium in the bottom chamber, and migrated cells were counted after 3 hours by flow cytometry. Migration index was calculated as the ratio of cells that transmigrated through the insert in the chemokine-treated cells vs untreated ones (CXCR11-/CXCR12-).


### 3.6. CXCR3 On CLL Cells as a Prognostic Marker

To study the prognostic value of studied markers, we constructed ROC curves for CLL patients with favourable (IgHVmut) and unfavourable (IgHVunmut) prognostic groups. Among the studied markers, CXCR3 had the highest sensitivity and specificity on both CD5high and CD5low populations. Cut-off values for CXCR3 were 65.2%, 24.0%, and 54.8% for CD5high, CD5low, and CLL cells as a whole, respectively. Correspondingly, AUC reached values of 0.810, 0.859, and 0.763 on CD5high, CD5low, and CLL cells as a whole (Figure 6(a)). Kaplan-Meier curves using CXCR3 cut-off values showed the prognostic value of CXCR3 on CD5low (P=0.030) on time to the next treatment, calculated from the sampling time (Figure 6(b)). For the analysis, only patients with sufficient follow-up time were included.Figure 6
(a) Receiver operating characteristic (ROC) curves created for the percentage of CXCR3 positive all CLL cells, CD5low and CD5high cells, respectively, in patients with IgHVmut and IgHVunmut status. AUC: area under ROC curve. (b) Kaplan-Meier curves of time to next treatment in CLL patients according to the cut-off values of the percentage of CXCR3 on CD5low and CD5high cells.
(a)(b)


### 3.7. Multivariate Patient Similarity Networks

To gain more insights into CD5high and CD5low subpopulations in IgHVmut and IgHVunmut patients, we constructed the multivariate PSNs and performed their clustering based on the CXCR3, CXCR4, CXCR5, and CCR7 expression in enrolled CLL patients. Clusters with high CXCR3 include predominantly patients with IgHVmut status, and vice versa clusters with low CXCR3 include predominantly patients with IgHVunmut status. CXCR3 and other selected markers on CD5low subpopulations better discriminate between patients with IgHVmut and IgHVunmut subgroups than markers on CD5high (Figure 7). For clustering and distribution of chemokine expression in particular clusters and corresponding expression patterns, see the Supplementary file (Figure S4).Figure 7
Patient similarity networks for the combination of chemokine expression (% of CXCR3, and MFI of CXCR4, CXCR5, CCR7) on (a) CD5high and (b) CD5low subpopulations in CLL patients. The nodes represent individual CLL patients, the lines connect the patients with high similarity in chemokine profiles. IgHVmut patients are presented in green, IgHVunmut patients in red. CXCR3 positive cells with cut-off <65% for CD5high and <24% for CD5low, respectively, are orange, and CXCR3 positive cells with cut-off >65% for CD5high and >24% for CD5low are blue.
(a)(b)


## 3.1. CD5/CD19 Markers as Identifiers of CD5high and CD5low Cells

First, we identified CD5high and CD5low cells based on the CD5/CD19 model and compared it with the reported CD5low/CXCR4high model [8, 9]. The CD5high cells in the CD5/CD19 model corresponded to those in the CD5high/CXCR4low model, and similarly CD5low cells corresponded to those in the CD5low/CXCR4high one (Figure S1A). High interindividual variability in the proportion of both closely related subpopulations of CD5high and CD5low cells was observed, irrespective of IgHV mutational status and other clinical characteristics (Figure S1B).


## 3.2. Differential Expression of Chemokine Receptors and Adhesion Molecules on CD5high and CD5low CLL Cells

The expression of CD54, CD62L, CD49d, CCR5, CCR7, CCR10, CXCR3, CXCR4, and CXCR5 was evaluated on CD5high and CD5low subpopulations (Table S1).When analysing levels of studied markers (MFI) in CD5high and CD5low subpopulations, the CD5high cells expressed higher levels of CXCR5 (P=0.005) and CCR7 (P=0.013) and lower levels of CXCR4 receptor (P<0.001) than the population of CD5low cells. Besides, more CD5high cells were positive for CXCR3 (P<0.001), CCR5 (P=0.012), CCR10 (P=0.007), and CD62L (P=0.047) than CD5low cells (Figure 2, Table S1).Figure 2
Distribution of (a) percentages of positive cells for CXCR3, CCR10, and CD62L and (b) expression (MFI) of CXCR4, CXCR5, and CCR7 on CD5high and CD5low subpopulations in patients with CLL. Group means are indicated by horizontal bars, error bars indicate 95% CI.
(a)(b)


## 3.3. Characterization of CD5high and CD5low CLL Cells in Patient Subgroups according to the IgHV Mutational Status

To characterize the CLL cells and their subpopulations in patient subgroups according to theIgHV mutational status, we compared the expression of studied markers on CLL cells as a whole and separately on CD5high and CD5low cells in CLL patients with IgHVmut and IgHVunmut status (Figure 3, Table S2, Table S3).Figure 3
Distribution of CXCR3 and CXCR4 chemokines inIgHVmut (violet) and IgHVunmut (red) patients in (a) CLL cells as a whole, (b) CD5high, and CD5low subpopulations in a paired analysis. The lines connect the values on CD5high and CD5low subpopulations in the same patient.
(a)(b)Of the studied markers, patients withIgHVmut had a higher percentage of cells expressing CXCR3 (P=0.003, Figure 3(a)) and CD62L (P=0.003) in the whole population of CLL cells compared to those with IgHVunmut status. When IgHVmut and IgHVunmut patients were evaluated separately, in both groups, a higher proportion of CLL cells positive on CXCR3 (in both P<0.001) and lower expression of CXCR4 (in both P<0.001) was observed on CD5high subpopulation in comparison with CD5low cells (Figure 3(b)). Similarly, when CD5high and CD5low cell populations were evaluated separately, the IgHVmut group exhibited higher percentages of CXCR3 (in both P<0.001), CD62L (in both P=0.003) positive cells, as well as higher expression of CXCR5 (P<0.001 and P=0.011, respectively) in comparison with IgHVunmut (Table S2, Table S3).To exclude possible influence of the treatment on the studied parameters, we performed subanalysis on a cohort of untreated patients subdivided according to theIgHV mutational status, and we confirmed the differences for studied markers observed in the whole patient cohort (Figure S2A). Moreover, we did not observe significant differences in studied markers between IgHVunmut patients untreated and those with treatment history (Figure S2B).


## 3.4. Correlation of CXCR3, CXCR4, and CXCR5 with CD5 Expression on CLL Cells

On CLL cells, CD5 expression (MFI) positively correlated with the percentages and MFI of CXCR3 (rs=0.54, P<0.001 and rs=0.54, P<0.001) and MFI of CXCR5 (rs=0.34, P=0.010). There was a trend towards inverse correlation between CD5 and CXCR4 expression on the whole CLL subpopulation (rs=−0.23, P=0.086) (Figure 4(a)). Further information about correlations of CXCR3, CXCR4, and CXCR5 expression on CD5high and CD5low subpopulations is provided in the Supplementary file.Figure 4
Correlation of CD5 expression with (a) percentages of CXCR3, levels (MFI) of CXCR4 and CXCR5 on CLL cells, and (b) studied chemokines on CLL cells assessed by the correlation network analysis.
(a)(b)Regarding the relationship between CXCR3 and CXCR4, correlation analysis revealed a negative correlation between percentages as well as expression (MFI) of CXCR3 with CXCR4 on CLL cells (rs=−0.35, P=0.009 and rs=−0.38, P=0.006, respectively) (Figure S3). Network correlation analysis among studied chemokines and CD5 further confirmed a relationship and importance of CD5-CXCR3-CXCR5 axis on CLL cells (Figure 4(b)).


## 3.5. Migration Rate of CLL Cells in the Presence of CXCL11

To analyse the cooperative interplay between CXCR3 and CXCR4, we analysed the migratory ability of CXCL11-treated and untreated CLL cells towards chemokine CXCL12. The highest migration rates were observed for the CXCL11-untreated cells that migrated towards CXCL12 (P=0.010) (Figure 5). When CLL cells were treated with CXCL11, their spontaneous migration as well as migration rate toward CXCL12 decreased (Figure 5).Figure 5
Cooperative effect of CXCL11 and CXCL12 on the migratory ability of CLL cells. CLL cells (n=6), with/without pretreatment with 200 ng/mL CXCL11, were added to the upper chamber of Transwell inserts. 200 ng/mL CXCL12 was added to the medium in the bottom chamber, and migrated cells were counted after 3 hours by flow cytometry. Migration index was calculated as the ratio of cells that transmigrated through the insert in the chemokine-treated cells vs untreated ones (CXCR11-/CXCR12-).


## 3.6. CXCR3 On CLL Cells as a Prognostic Marker

To study the prognostic value of studied markers, we constructed ROC curves for CLL patients with favourable (IgHVmut) and unfavourable (IgHVunmut) prognostic groups. Among the studied markers, CXCR3 had the highest sensitivity and specificity on both CD5high and CD5low populations. Cut-off values for CXCR3 were 65.2%, 24.0%, and 54.8% for CD5high, CD5low, and CLL cells as a whole, respectively. Correspondingly, AUC reached values of 0.810, 0.859, and 0.763 on CD5high, CD5low, and CLL cells as a whole (Figure 6(a)). Kaplan-Meier curves using CXCR3 cut-off values showed the prognostic value of CXCR3 on CD5low (P=0.030) on time to the next treatment, calculated from the sampling time (Figure 6(b)). For the analysis, only patients with sufficient follow-up time were included.Figure 6
(a) Receiver operating characteristic (ROC) curves created for the percentage of CXCR3 positive all CLL cells, CD5low and CD5high cells, respectively, in patients with IgHVmut and IgHVunmut status. AUC: area under ROC curve. (b) Kaplan-Meier curves of time to next treatment in CLL patients according to the cut-off values of the percentage of CXCR3 on CD5low and CD5high cells.
(a)(b)


## 3.7. Multivariate Patient Similarity Networks

To gain more insights into CD5high and CD5low subpopulations in IgHVmut and IgHVunmut patients, we constructed the multivariate PSNs and performed their clustering based on the CXCR3, CXCR4, CXCR5, and CCR7 expression in enrolled CLL patients. Clusters with high CXCR3 include predominantly patients with IgHVmut status, and vice versa clusters with low CXCR3 include predominantly patients with IgHVunmut status. CXCR3 and other selected markers on CD5low subpopulations better discriminate between patients with IgHVmut and IgHVunmut subgroups than markers on CD5high (Figure 7). For clustering and distribution of chemokine expression in particular clusters and corresponding expression patterns, see the Supplementary file (Figure S4).Figure 7
Patient similarity networks for the combination of chemokine expression (% of CXCR3, and MFI of CXCR4, CXCR5, CCR7) on (a) CD5high and (b) CD5low subpopulations in CLL patients. The nodes represent individual CLL patients, the lines connect the patients with high similarity in chemokine profiles. IgHVmut patients are presented in green, IgHVunmut patients in red. CXCR3 positive cells with cut-off <65% for CD5high and <24% for CD5low, respectively, are orange, and CXCR3 positive cells with cut-off >65% for CD5high and >24% for CD5low are blue.
(a)(b)


## 4. Discussion

There is a growing body of evidence that CLL neoplastic cells are composed of subpopulations of cells that differ in their biological function [1, 2, 8, 19, 20]. Our study revealed differences in the expression of molecules linked to adhesion, extravasation, migration, and homing, on CD5high and CD5low CLL populations, defined using the CD5/CD19 coexpression model, in the patient subgroups according to the IgHV mutational status.In our CLL cohort, we observed high expression levels of CXCR3, CXCR5, CCR10, and CD62L on CD5high cells and high CXCR4 on CD5low cells, which is in line with a previous study [8]. Interestingly, our data showed that CXCR3 better discriminates both CD5high and CD5low cell populations than CXCR4, a key chemokine receptor involved in migration of CLL cells to the supportive lymphoid tissues [1, 21, 22]. Moreover, our study revealed differences in expression pattern between patient subgroups according to the IgHV mutational status, a key prognostic predictor of overall survival and treatment-response duration [10–12]. The most prominent were the differences between the percentages of CXCR3 on CD5low cell subpopulation between IgHVmut and IgHVunmut statuses. Moreover, our results revealed that CXCR3 on CD5low cells has the best prognostic utility in discriminating patients with IgHVmut and IgHVunmut status, and correspondingly the patients with favourable or poor prognosis. This observation contributes to further characterization of IgHVmut and IgHVunmut mutation statuses, known to differ in gene expression [23], methylation status [24], and the mutational landscape [25].Recently, CXCR3 has been found as a marker of independent prognostic significance in CLL [26]. High CXCR3 expression and CXCR3/CXCR4 ratio delineated patients with a significantly better clinical course, as opposed to patients with low CXCR3 and high CXCR4 expression [26, 27]. To date, there is no clear understanding of how CXCR3 influences the pathogenesis of CLL. A formation of CXCR3-CXCR4 heteromers and a negative binding cooperativity between CXCR3 and CXCR4 at the cell surface was reported on CLL [26] and HEK293T [28] cells. Importantly, the CXCR3-CXCR4 heteromerization has been shown to alter the ligand-binding kinetics: CXCR3 and CXCR4 agonists have been proved to inhibit each other’s equilibrium binding on membranes and specifically accelerate dissociation of CXCL12 from CXCR4 [28]. The negative impact of CXCR3 stimulation by its ligands CXCL9, CXCL10, and CXCL11 was shown to be highly specific for CXCR4-induced migration and resulted in reduced CXCR4-CXCL12 chemotaxis [26], as we also confirmed by the migration experiment in our patients. Given the critical role of CXCR4-CXCL12 axis in migration of CLL cells between blood and supportive lymphoid tissues in CLL, the formation of CXCR3-CXCR4 heterodimers on CLL cells and its consequences may significantly reduce the migration of CLL cells [1, 21, 22]. Since the proportion of CXCR3-CXCR4 heteromers is relative to homomers of both receptors [28], low levels of CXCR3 on CLL cells, observed in our patients with mutated IgHV status, might not be sufficient to abrogate migration of CLL cells driven by CXCR4. Contribution of CXCR3 to better prognosis and attenuation of CLL cell migration deserves future investigations.Our study introduced a simple model based on CD5/CD19 coexpression for studying the CLL subpopulations. Given comparable data to a study using markers CD5/CXCR4 [8], CD5/CD19 coexpression might represent a combination of markers capable of reflecting biological differences between CLL clones, an assumption that needs to be verified in future studies. Moreover, the fact that the subpopulation of CLL cells in the CD5/CD19 model correspond to those in the CD5/CXCR4 model suggests that CD5 may play a more important pathogenic role in CLL than previously recognized. The precise function of CD5 in the interactions of immune cells remains unclear, especially on CLL cells. It was shown that this molecule negatively regulates B1 cell activation and activation-induced cell death [29, 30]. There is growing evidence of several pools of leukemia cells present in CLL, including circulating cell cycle arrested CLL cells expressing preferably low levels of CD5, and migrated activated cells that express high levels of CD5 and that are driven to proliferate via signals from the lymphoid tissue microenvironment [2, 8, 31, 32]. Despite this, CD5 positive cells were shown to have a longer lifespan than CD5 negative cells [29]. Whether CD5 contributes also to the recirculating capacity of CLL cells and their differential proliferative potential deserves further investigations. A deeper understanding of the CD5 role in CLL clones’ biology may permit potentiation of current immunotherapeutic strategies.The study has several limitations. First, we analysed a diagnostic real-world CLL cohort of patients sampled at different time points and treatment regimes. Second, our exploratory study should be followed by functional investigations on the role of CXCR3 on CD5high and CD5low cell populations in future studies. Despite these limitations, we believe that our novel model for distinguishing between proliferative and resting fractions of neoplastic cells and first study on characteristics of CLL subpopulations in CLL patients with different IgHV mutational statuses highlights the critical contribution of chemokine receptors to the disease outcome in CLL.In summary, we present for the first time the marked differences in expression of chemokine receptor CXCR3 on CD5high and CD5low cell populations in patients with different IgHV mutational statuses. The wide presence of CXCR3 marker on CLL cells appears to portend a favourable prognosis, thus further supporting its potential as a prognostic marker. Understanding the pathological relevance of CD5high and CD5low cell subsets, their characteristics and phenotypes may likely broaden our understanding of CLL pathology as well as reveal novel therapeutic avenues.



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