# Deuterium Isotope Effects onN14,15 Chemical Shifts of Ammonium Ions: A Solid State NMR Study

Authors: Poul Erik Hansen
Journal: International Journal of Inorganic Chemistry
 (2011)

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## Abstract

Deuterium isotope effects onN14,15 chemical shifts are measured in ammonium halides in the solid state using both enriched N15 salts and N14 natural abundance materials. The effects are correlated to N15 chemical shifts and to N···X distances. The deuterium isotope effects on N14,15 chemical shifts in the solid state are discussed in relation to effects observed in solution. No NH couplings are seen due to fast rotation in the solid, which leads to self-decoupling, whereas ND couplings are present.


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## Body

## 1. Introduction

Ammonium ions both in solution and in the solid state show extraordinary properties as expressed by a very fast rotation in solution [1] and in the solid by unusual structures both of the ammonium salts themselves [2] but also of the deuteriated species [3].The ammonium chloride, bromide, and iodide exist as CsCl structures at ambient temperature [4]. The nitrogen to halide distances are given in a couple of reference papers [5, 6].Isotope effects of ammonium salts in solution show counter ion-dependent chemical shifts both on1H and 14,15N chemical shifts [7]. The smallest effects for different salts were found in very dilute solutions [7]. Studies of ammonium ions in crown ethers and cryptands showed for ions fully embedded in a cryptand no counter ion dependence [8]. Theoretical calculations on ammonium ions surrounded by water or ammonia showed a dependence on heavy atom distance for deuterium isotope effects on nitrogen or hydrogen chemical shifts [8, 9]. Deuterium isotope effects in the solid state have been demonstrated for ammonium chloride [10]. Recent advances in solid state NMR have made it possible to measure 14N solid state spectra of ammonium salts [11, 12].The present study investigates deuterium isotope effects on nitrogen chemical shifts in order to elucidate the dependence on the heavy atom distance and direction to get a firmer basis for interpretation of deuterium isotope effects on nitrogen chemical shifts also in solution.


## 2. Results

The primary goal has been to measure deuterium isotope effects on nitrogen chemical shifts of ammonium salts in the solid. The experiments have been done using fully deuteriated compounds mixed with the nondeuteriated species. In order to measure the isotope effects, a number of experiments have been done on14N or 15N ammonium salts using both proton decoupling and no proton decoupling.The signal from the fully deuteriated species is a nonet. The chemical shift is that of the highest centre peak (marked on Figures1(a) and 1(b)). The full deuterium isotope effect on the chemical shift is given as the difference between the chemical shift of the NH4 and the ND4 peaks.(a) Solid state15N spectrum of a 1 : 1 mixture of NH4Cl and ND4Cl. The six highest peaks have the frequencies 0 ppm (NH4+) and −1.555, −1.330, −1.506, −1.694, and −1.870 ppm (ND4+). (b) Solid state 14N spectrum of a 1 : 1 mixture of NH4Br and ND4Br (with a small amount of ND3HBr). For the five highest peaks, the frequencies are 2.227 ppm (NH4+) 0.977, 0.783, 0.607, and 0.432 ppm (ND4+).
(a)(b)


### 2.1.N15 Spectra

For the15N observation, spectra were typically recorded with a spinning speed of 12000 Hz in order to achieve good resolution. The line widths for the NH4+ peak 18 Hz and for the ND4+ species slightly better (see Figure 1(a)). A common feature for all proton-coupled spectra is a lack of observation of NH couplings.


### 2.2.N14 Spectra

Magic angle spinning spectra without proton decoupling of ammonium chloride show sharp resonances (line width 42 Hz), but no splittings due to NH couplings. Proton decoupling led to a sharpening of the resonances (line width 17 Hz). This holds true for a spinning speed range between 7000 and 12000 Hz. For the corresponding perdeuteriated compounds primarilyND4+ with a small amount of ND3H+ showed resolved ND couplings at spinning speeds above 10000 Hz. Isotope effects were typically measured in 1 : 1 mixtures of NH4 and ND4 salts (Figures 1(a) and 1(b)). For ammonium chloride, the 14ND coupling constant is 7.6 ± 0.8 Hz and the Δ1N(D)4 isotope effect is measured as 1.61 ppm. For ammonium bromide, the isotope effect is measured as 1.62 ppm and the coupling constant is 7.6 ± 0.8 Hz, whereas the ammonium iodide gave Δ1N(D)4 = 1.81 ppm, no resolved couplings could be seen.


### 2.3.  Nitrogen Chemical Shifts

The nitrogen chemical shifts are −26, 0, 2.3, and 15.9 ppm for the F−, Cl−, Br−, and I−, respectively, as reported earlier [14]. 14N spectra of ammonium chloride cooled to −44°C showed a slight change in chemical shifts at −30°C in line with a phase transition at this temperature [4].


## 2.1.N15 Spectra

For the15N observation, spectra were typically recorded with a spinning speed of 12000 Hz in order to achieve good resolution. The line widths for the NH4+ peak 18 Hz and for the ND4+ species slightly better (see Figure 1(a)). A common feature for all proton-coupled spectra is a lack of observation of NH couplings.


## 2.2.N14 Spectra

Magic angle spinning spectra without proton decoupling of ammonium chloride show sharp resonances (line width 42 Hz), but no splittings due to NH couplings. Proton decoupling led to a sharpening of the resonances (line width 17 Hz). This holds true for a spinning speed range between 7000 and 12000 Hz. For the corresponding perdeuteriated compounds primarilyND4+ with a small amount of ND3H+ showed resolved ND couplings at spinning speeds above 10000 Hz. Isotope effects were typically measured in 1 : 1 mixtures of NH4 and ND4 salts (Figures 1(a) and 1(b)). For ammonium chloride, the 14ND coupling constant is 7.6 ± 0.8 Hz and the Δ1N(D)4 isotope effect is measured as 1.61 ppm. For ammonium bromide, the isotope effect is measured as 1.62 ppm and the coupling constant is 7.6 ± 0.8 Hz, whereas the ammonium iodide gave Δ1N(D)4 = 1.81 ppm, no resolved couplings could be seen.


## 2.3.  Nitrogen Chemical Shifts

The nitrogen chemical shifts are −26, 0, 2.3, and 15.9 ppm for the F−, Cl−, Br−, and I−, respectively, as reported earlier [14]. 14N spectra of ammonium chloride cooled to −44°C showed a slight change in chemical shifts at −30°C in line with a phase transition at this temperature [4].


## 3. Discussion

The finding that no NH couplings could be seen is due to fast rotation of the ammonium ion in the solid leading to self-decoupling as seen for adamantane [15]. In support of such a suggestion is the finding that the spectra become sharper at high spinning speeds and become very complex at low spinning speeds. Chemical exchange can be excluded as no major changes are seen as a function of cooling to temperatures as low as −44°C. The reason that ND couplings are seen but not those of NH can be ascribed to the fact that homonuclear couplings are much smaller for DD than for HH. The importance of homonuclear couplings were demonstrated for adamantane for which the NH couplings could be reestablished by quenching of the self-decoupling by means of strong off-resonance rf-irradiation of the protons [15].The isotope effects in the solid are seen to increase in the series ND4Cl~ND4Br < ND4I. If we furthermore include the data for the cryptand SC-24 [8], we find a decent correlation between Δ1N(D)4 and the 15N chemical shifts (Figure 2). Knowing the chemical shifts of NH4F [14] we can now estimate Δ1N(D)4 to 1.16 ppm. Using the nitrogen to acceptor distances, acceptor being either halide or N (for SC-24), we see again a decent correlation (Figure 3), but slightly different for the solid state halide data and those of SC-24 and for ammonium ions solvated by water (the latter two marked by triangles). The water distance is obtained from [16–18]. This distance is a matter of debate, but, whether one is using 2.78 or 3.08 Å, we see that water is more effective in reducing Δ1N(D)4 than halide ions.Figure 2
Plot of14,15N chemical shifts versus Δ1N(D)4. Data for SC-24 from [8].Figure 3
Plot ofRN⋯X versus Δ1N(D)4. The distance for SC-24 is given as an average of the distances found in [13].The slopeΔ1N(D)4/δN is 0.017 ppm/Å (Figure 2). This can be compared with solution in which it is 0.06 [7]. This indicates a quite different origin of the isotope effects in the liquid and solid state. In the solid state, the isotope effects decrease as the heavy atom distance is shortened (Figure 3). This is also supported by calculations [9, 19]. The heavy atom distance is clearly important but most likely in an indirect way as the nitrogen chemical shift has been shown to depend much more strongly on the NH bond length than on the heavy atom distance [14]. However, indirectly, the heavy atom distance will determine the interaction potential and the NH bond length.In the liquid state, the largest isotope effects were found for high concentrations of iodide ions [7]. In contrast, the smallest Δ1N(D)4 is found in very dilute solutions [7] as a function of the inner water solvation shell leading to short N⋯O distances. At higher concentration of halide ions, the waters will be partly displaced by halide ions but at a longer heavy atom distance leading to the observed increase in Δ1N(D)4 both because of this and because halide ions are less effective in reducing the isotope effects (see earlier).Ammonium ions are also a good model for positively charged lysine molecules. For hydrated lysines, aΔ1N(D)3 of 1.05 ppm is found [20]. The isotope effects per deuterium are only slightly different for ammonium ions (0.30 ppm) and for lysines (0.35 ppm). Deuterium isotope effect on 14,15N chemical shifts may be used to gauge the amount of solvation in biological systems.


## 4. Experimental

Ammonium salts were deuteriated by repeated dissolution in D2O followed by evaporation under reduced pressure.The15N spectra were recorded on 90%  15N enriched ammonium chloride purchased from Aldrich. All solid state NMR spectra were measured on a Varian 600 Inova instrument using magic angle spinning.The14N MAS NMR spectra were acquired at 14.1 T using a homebuilt 5 mm CP/MAS probe for 5 mm o.d. rotors, a spinning speed of νR=12.0 kHz, single-pulse excitation with a 90° pulse (γB1/2π=45 kHz), a 4-s repetition delay, and 512 scans. Acquisition time is 0.2 s, spectral width 100000 Hz, and number of points 40000.The15N MAS spectra were recorded without cross-polarization at 14.1 T (νR=12.0 kHz and γB1/2π=50 kHz) using a homebuilt 4 mm CP/MAS probe for 4 mm o.d. rotors, a 30-s relaxation delay, and 64 scans. Acquisition time is 0.1 s, spectral width 100000 Hz, and number of points 20032. Acoustic ringing caused no significant problems in either of the experiments due to the narrow resonances observed in both spectra.



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*Source: 696497-2011-12-27.xml*