# Algae Mediated Green Fabrication of Silver Nanoparticles and Examination of Its Antifungal Activity against Clinical Pathogens

Authors: Shanmugam Rajeshkumar; Chelladurai Malarkodi; Kanniah Paulkumar; Mahendran Vanaja; Gnanadas Gnanajobitha; Gurusamy Annadurai
Journal: International Journal of Metals
 (2014)

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## Abstract

Algae extract has the great efficiency to synthesize the silver nanoparticles as a green route. Brown seaweed mediates the synthesis of silver nanomaterials using extract ofSargassum longifolium. For the improved production of silver nanomaterials, some kinetic studies such as time incubation and pH were studied in this work. 10 mL of algal extract was added into the 1 mM AgNO3 aqueous solution. The pH and reaction time range were changed and the absorbance was taken for the characterization of the nanoparticles at various time intervals, and the high pH level shows the increased absorbance due to the increased nanoparticles synthesis. The synthesized silver nanoparticles were characterized by Scanning Electron Microscope (SEM) showing that the shape of the material is spherical, and X-Ray Diffraction value obtained from range of (1 1 1) confirmed synthesized silver nanoparticles in crystalline nature. TEM measurement shows spherical shape of nanoparticles. The Fourier Transmittance Infrared spectrum (FT-IR) confirms the presence of biocomponent in the algae extract which was responsible for the nanoparticles synthesis. The effect of the algal mediated silver nanoparticles against the pathogenic fungi Aspergillus fumigatus, Candida albicans, and Fusarium sp. S. longifolium mediated synthesized silver nanoparticles shows cheap and single step synthesis process and it has high activity against fungus. This green process gives the greater potential biomedical applications of silver nanoparticles.


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## Body

## 1. Introduction

Seaweeds are the natural and renewable living resources in the marine ecosystem and they are consumed for food, feed, and medicine. Seaweeds contain more than 60 elements, macro- and micronutrients, proteins, carbohydrates, vitamins, and aminoacids [1]. Seaweeds are the sources for extracting industrial products such as phycocolloids: carrageenan, alginates, and agar [2, 3]. Sargassum is a big family of marine brown algae and it has a broad application field. Most of the seaweeds have the antibacterial activity against pathogenic bacteria like Vibrio parahaemolyticus, Salmonella sp., Shewanella sp., Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, andProteus mirabilis [4], antibiotic resistant postoperative infectious pathogens [5], and also used as antitumor compounds [6]. Moreover, seaweeds play an important role in adsorption of heavy metals like lead, copper, zinc, and manganese [7].In the 21st century, nanotechnology is the newly emerging multidisciplinary research area with synthesis of nanosized materials [8]. Nanotechnology is the manipulation and production of materials ranging in size from 1 to 100 nanometer scale [9]. The nanoparticles can play a topmost role in the field of nanomedicines such as health care and medicine diagnostic and screening purposes, drug delivery systems, antisense and gene therapy applications, and tissue engineering and expectations of nanorobots configuration [10]. Many methods adopted in the field of synthesis of nanoparticles are chemical and physical. Nowadays, biological method of nanoparticles synthesis is a vast growing technique in the field of nanotechnology [11]. The biological sources had the more quantity of trouble-free protocols and when applied for the human health associated field, it is easy to approach for maintain aseptic environment during the synthesis process of nanoparticles [12]. Recently, biological materials such as bacteria, fungi, plant, and algae were used to synthesis of nanoparticles. Some are Enterobacteria[13], Aspergillus fumigatus[14], Coriander leaf [15], and Sargassum wightii [16] were used to synthesise of nanoparticles. Among the biological materials, algae is called as “bionanofactories” because the live and dead dried biomass was used for synthesis of metallic nanoparticles. It is low cost and environmentally effective, macroscopic structured material, and has the distinct advantage due to its high metal uptake capacity [17]. The rate and size of the nanoparticles were controlled by optimizing the parameters such as pH, temperature, substrate concentration, and incubation time [18].This study reported that the exposure time and pH play an important role in the controlling of nanoparticles synthesis by using theS. longifoliumalgae extract. Nanoparticles synthesis was characterized by UV-vis spectroscopy, crystalline, and morphological structure which were characterized by XRD, SEM, and TEM. The antifungal activity of silver nanoparticles against pathogenic fungus was studied as well.


## 2. Materials and Methods

### 2.1. Chemicals

Analytical grade chemicals are silver nitrate, sodium hydroxide, and hydrochloric acid used for preparation of silver nanoparticles (NPs) and role of pH on NPs synthesis. Agar agar, Rose Bengal agar, and Sabouraud Dextrose agar were used for assessment of antifungal activity. All the chemicals and media were purchased from HiMedia (Mumbai, India).


### 2.2. Collection and Preparation of Algal Extract

The brown algaeSargassum longifolium was collected from the Tuticorin coastal area, Tamilnadu, India. The marine brown seaweed was thoroughly washed with fresh water and distilled water to remove the salt minerals and metallic compounds on the surface of the seaweed. Clean seaweed was dried at a shady place for ten days. The dried leaves were ground into fine powder. 1 gm of algal powder was mixed with 100 mL of distilled water in the 250 mL Erlenmeyer flask and boiled at 60°C for 10 min. The boiled extract was filtered through Whatman No. 1 filter paper, collected the supernatant, and stored at 4°C for nanoparticles synthesis.


### 2.3. Green Synthesis of Silver Nanoparticles

Typically, 10 mL of pure algal extract solution was mixed with aqueous solution of 90 mL of 1 mM silver nitrate (AgNO3) solution and kept in room temperature with constant stirring at 120 rpm. A color change of the solution was noted by visual inspection and UV-vis spectroscopy at different time and wavelength confirming the synthesis of silver nanoparticles.


### 2.4. Effect of pH

The role of pH in the synthesis of silver nanoparticles was carried out by altering the pH of algal extract. The pH range was varied from 6.2, 6.8, 7.8 and 8.4 by using analytical graded 0.1 N sodium hydroxide and 0.1 N hydrochloric acid standard solutions. The influence of pH on the synthesis process was analyzed by UV-vis spectrophotometer in the wavelength range of 380–600 nm.


### 2.5. Purification and Characterization of Synthesized Silver Nanoparticles

The bioreduction of silver ions in aqueous solution using algae extract was monitored by double beam UV-vis spectrophotometer at different wavelengths from 320 to 700 nm (Perkin Elmer, Singapore). Green synthesized silver nanoparticles were purified by distilled water by repeated centrifugation at 10,000 rpm for 15 min. Crystalline nature of the purified silver nanoparticles was analyzed by XRD (Bruker, Germany, model: D8Advance) and particle morphology was characterized by Scanning Electron Microscope (Hitachi, Model: S-3400N). The functional biomolecules such as carboxyl groups present in the seaweed responsible for the silver nanoparticles formation were characterized by FT-IR (BrukerOptik GmbH Model No.—Tensor 27). The dried silver nanoparticles were compressed with KBr into thin pellets and measured at the wavelength range from 4000 to 400 cm−1.


### 2.6. Antifungal Assay of Silver Nanoparticles

#### 2.6.1. Clinical Fungal Pathogens

The three fungal pathogenic strains used in the present study were isolated from clinical samples and identified from Microlabs, Vellore District, India, which wereAspergillus fumigatus, Candida albicans, and Fusariumsp.


#### 2.6.2. Assay of Antifungal Activity

The antifungal activity of green synthesized silver nanoparticles against various fungal strains was assayed by Agar well diffusion method. The fungicidal effect of the silver nanoparticles could be assessed by the formation of zone around the well. 100 mL of sterilized Sabouraud Dextrose Agar medium was poured into three sterilized Petri dishes. The fungal strains were grown in Rose Bengal agar and their spores were mixed into the 10 mL sterile distilled water and swapped on the agar. Three wells of 5 mm diameter were prepared and loaded with silver nanoparticles at different concentrations (30, 60, and 90μL). The plates loaded with the fungal and silver nanoparticles were incubated at 37°C. The antifungal activities against the fungal strains were confirmed by forming the zone around the wells and measured after 24 hrs of incubation. The zone of inhibition was expressed in mm in diameter. The experiments were repeated three times to find the standard deviation and standard error.


## 2.1. Chemicals

Analytical grade chemicals are silver nitrate, sodium hydroxide, and hydrochloric acid used for preparation of silver nanoparticles (NPs) and role of pH on NPs synthesis. Agar agar, Rose Bengal agar, and Sabouraud Dextrose agar were used for assessment of antifungal activity. All the chemicals and media were purchased from HiMedia (Mumbai, India).


## 2.2. Collection and Preparation of Algal Extract

The brown algaeSargassum longifolium was collected from the Tuticorin coastal area, Tamilnadu, India. The marine brown seaweed was thoroughly washed with fresh water and distilled water to remove the salt minerals and metallic compounds on the surface of the seaweed. Clean seaweed was dried at a shady place for ten days. The dried leaves were ground into fine powder. 1 gm of algal powder was mixed with 100 mL of distilled water in the 250 mL Erlenmeyer flask and boiled at 60°C for 10 min. The boiled extract was filtered through Whatman No. 1 filter paper, collected the supernatant, and stored at 4°C for nanoparticles synthesis.


## 2.3. Green Synthesis of Silver Nanoparticles

Typically, 10 mL of pure algal extract solution was mixed with aqueous solution of 90 mL of 1 mM silver nitrate (AgNO3) solution and kept in room temperature with constant stirring at 120 rpm. A color change of the solution was noted by visual inspection and UV-vis spectroscopy at different time and wavelength confirming the synthesis of silver nanoparticles.


## 2.4. Effect of pH

The role of pH in the synthesis of silver nanoparticles was carried out by altering the pH of algal extract. The pH range was varied from 6.2, 6.8, 7.8 and 8.4 by using analytical graded 0.1 N sodium hydroxide and 0.1 N hydrochloric acid standard solutions. The influence of pH on the synthesis process was analyzed by UV-vis spectrophotometer in the wavelength range of 380–600 nm.


## 2.5. Purification and Characterization of Synthesized Silver Nanoparticles

The bioreduction of silver ions in aqueous solution using algae extract was monitored by double beam UV-vis spectrophotometer at different wavelengths from 320 to 700 nm (Perkin Elmer, Singapore). Green synthesized silver nanoparticles were purified by distilled water by repeated centrifugation at 10,000 rpm for 15 min. Crystalline nature of the purified silver nanoparticles was analyzed by XRD (Bruker, Germany, model: D8Advance) and particle morphology was characterized by Scanning Electron Microscope (Hitachi, Model: S-3400N). The functional biomolecules such as carboxyl groups present in the seaweed responsible for the silver nanoparticles formation were characterized by FT-IR (BrukerOptik GmbH Model No.—Tensor 27). The dried silver nanoparticles were compressed with KBr into thin pellets and measured at the wavelength range from 4000 to 400 cm−1.


## 2.6. Antifungal Assay of Silver Nanoparticles

### 2.6.1. Clinical Fungal Pathogens

The three fungal pathogenic strains used in the present study were isolated from clinical samples and identified from Microlabs, Vellore District, India, which wereAspergillus fumigatus, Candida albicans, and Fusariumsp.


### 2.6.2. Assay of Antifungal Activity

The antifungal activity of green synthesized silver nanoparticles against various fungal strains was assayed by Agar well diffusion method. The fungicidal effect of the silver nanoparticles could be assessed by the formation of zone around the well. 100 mL of sterilized Sabouraud Dextrose Agar medium was poured into three sterilized Petri dishes. The fungal strains were grown in Rose Bengal agar and their spores were mixed into the 10 mL sterile distilled water and swapped on the agar. Three wells of 5 mm diameter were prepared and loaded with silver nanoparticles at different concentrations (30, 60, and 90μL). The plates loaded with the fungal and silver nanoparticles were incubated at 37°C. The antifungal activities against the fungal strains were confirmed by forming the zone around the wells and measured after 24 hrs of incubation. The zone of inhibition was expressed in mm in diameter. The experiments were repeated three times to find the standard deviation and standard error.


## 2.6.1. Clinical Fungal Pathogens

The three fungal pathogenic strains used in the present study were isolated from clinical samples and identified from Microlabs, Vellore District, India, which wereAspergillus fumigatus, Candida albicans, and Fusariumsp.


## 2.6.2. Assay of Antifungal Activity

The antifungal activity of green synthesized silver nanoparticles against various fungal strains was assayed by Agar well diffusion method. The fungicidal effect of the silver nanoparticles could be assessed by the formation of zone around the well. 100 mL of sterilized Sabouraud Dextrose Agar medium was poured into three sterilized Petri dishes. The fungal strains were grown in Rose Bengal agar and their spores were mixed into the 10 mL sterile distilled water and swapped on the agar. Three wells of 5 mm diameter were prepared and loaded with silver nanoparticles at different concentrations (30, 60, and 90μL). The plates loaded with the fungal and silver nanoparticles were incubated at 37°C. The antifungal activities against the fungal strains were confirmed by forming the zone around the wells and measured after 24 hrs of incubation. The zone of inhibition was expressed in mm in diameter. The experiments were repeated three times to find the standard deviation and standard error.


## 3. Results and Discussion

### 3.1. Visual and UV-Vis Spectrophotometer Analysis

Reduction of silver ions to silver nanoparticles was visually identified by color change from yellow to brown in the aqueous solution of reaction mixture at 1 hr incubation time (Figure1). Brown formation occurred due to the oscillation of free electrons in the reaction mixture. The color change depended on the incubation time. The deep brown color for silver nanoparticles was attained at 32 hr indicating that the increasing of color intensity is directly proportion to the time of incubation. Furthermore, the nanoparticles formation by the algal extract was confirmed by UV-vis spectroscopy at different wavelengths. Similarly, Chandran et al. [19] synthesized silver nanoparticles using Aloe vera extract taking 24 hr of reaction time in the presence of ammonia which enhances the nanoparticles formation.Figure 1
Color change after the addition of algae extract with 1 mM AgNO3 indicates the formation of silver nanoparticles. (a) Initial color change, (b) 1 hr, (c) 32 hr.UV-vis spectroscopy analysis depends on the arising of color in the reaction due to the excitation of surface Plasmon resonance band in a reaction mixture and was recorded as different functional time. Figure2 shows that, there is no peak were formed at the initial stage indicates that there is no synthesis of silver nanoparticles was observed.  After 1 hr of incubation time the surface Plasmon resonance band for silver nanoparticles was positioned at around 460 nm and the synthesis was steadily increasing with the increasing in time of reaction without change in the peak position. It shows no peak variation in their position with broad band indicates presence of polydispersed nanoparticles. The nanoparticles synthesis was completed at 64 h time of incubation.Figure 2
UV-vis spectra recorded the formation of nanoparticles in the reaction mixture of algae extract and AgNO3 at different time intervals showing the peak at 460 nm.


### 3.2. Effect of pH

The pH of the reaction mixture plays an important role in the nanoparticles synthesis. At low pH 6.2, the color change of the reaction mixture was slower than that at high pH 8.4. The color intensity of the reduction process increased with the increase of the pH (Figure3). Figure 4 shows the UV-vis spectra to analyze the pH impact on the nanoparticles synthesis. At lower pH 6.2 and 6.8, the broadened SPR band was shown at 460 nm indicating polydispersed nanoparticles formation. Broadening peaks occurred at lower pH due to the excitations of longitudinal Plasmon vibrations [20]. Broad band at low pH is due to the formation of anisotropic nanoparticles [21]. The narrow peak at higher pH that occurred is due to the formation of monodispersed and small-sized silver nanoparticles [22]. Lower pH suppresses nanoparticles formation and higher pH enhances the nanoparticles synthesis process. The high absorbance and narrow band was formed at 440 nm in the higher pH. Similarly, some reports explained that pH plays an important role in shape and size control synthesis process of silver nanoparticles. This is also in agreement with earlier reports that the addition of an alkaline ion in the reaction mixture is necessary to carry out the reduction of metal ions [23].Color change variation at different pH levels (a) pH 6.2 (b) 6.8 (c) 7.8 (d) 8.4.
(a)
(b)
(c)
(d)Figure 4
Effect of pH on the synthesis of silver nanoparticles shows that maximum synthesis occurred at high pH.


### 3.3. XRD Analysis

The crystalline structure of the silver nanoparticles was determined by XRD in the whole spectrum of 2θ values ranging from 10 to 80. The green synthesized silver nanostructure by employing algae extract of S. longifoliumwas confirmed by the characteristic peaks observed in the XRD image (Figure 5). The four distinct diffraction peaks of the 2θ values of 38.27°, 44.16°, 65.54°, and 77.42° could be assigned the plane of (1 1 1), (2 0 0), (2 2 0), and (3 1 1), respectively, indicating that the silver nanoparticles are fcc (face centered cubic) and crystalline in nature. The synthesized silver nanoparticles are compared with standard silver nitrate and pure silver particles which are published by Joint Committee on Powder Diffraction Standards (File nos. 04-0783 and 84-0713). The XRD clearly shows that the four distinct planes for the silver nanoparticles synthesized from algal extract of S. longifoliumare highly crystallized and purified.Figure 5
XRD spectrum of silver nanoparticles synthesized fromS. longifoliumextract shows crystalline structure of nanoparticles.


### 3.4. SEM Analysis

SEM image shows the morphologies of the algae mediated synthesized bionanoparticles at different magnifications (Figure6). High density of silver nanoparticles is shown in the SEM image which is uniformly distributed on the surface of the cells of algae. The polydispersed silver nanoparticles were attached on the surface of the biomolecules of the algal cells. Silver nanoparticles adhered on the cells due to weakly dislodging of bound silver nanoparticles from the biomass and silver nitrate reaction mixture during preparation [24]. Similar results were obtained in the gold nanoparticles using algal extract of S. marginatum with the size ranging from 40 to 85 nm [25] and also by using the T. conoides algae [26]. It reveals the involvement of the polyphenolic or secondary capping agent in the nanoparticles formation.Scanning electron microscope image of silver nanoparticles synthesized fromS. longifoliumextract showing predominantly spherical shape nanoparticles on the surface of the cell at different magnifications: (a) 5 μm, (b) 3 μm, (c) 1 μm, and (d) 500 nm.
(a)
(b)
(c)
(d)


### 3.5. TEM Image of Silver Nanoparticles

The morphology ofS. longifoliumalgae extract mediated synthesized silver nanoparticles was determined by TEM. The synthesized silver nanoparticles were formed predominantly spherical and some are truncated and ellipsoidal in the form of agglomerates. Some of the nanoparticles noted that the edges of the particles were smoother than the centers (marked by arrows) in Figure 7. The edges of nanoparticles were shown smoother than the center indicating that the proteins in algal extract may cap the silver nanoparticles. Similarly, Ahmad et al. [27] suggested that proteins in basil plant are present among the particles and they adhered to their surfaces.Figure 7
TEM image of silver nanoparticles shows polydispersed synthesized silver nanoparticles.


### 3.6. FTIR

FTIR was used to identify the biomolecules inS. longifoliumresponsible for the silver ions reduction and stabilization of reduced silver ions (Figure 8). The broad spectrum at 3382 cm−1 shows the O–H bond stretching of alcohols and phenols. The narrow band at 1639 cm−1 (–NH–C=O) indicates the presence of N–H bended primary amines. A weak band at 1386 cm−1 corresponds to asymmetrical stretching for nitro compounds. The absorption peak at 1033 cm−1 illustrates the presence of carboxylic acid functional group. The very weak bands were formed at 613 and 504 cm−1 due to the occurrence of vibrations of alkyl halides. FTIR revealed that soluble organic compounds or proteins in the extract may bind with silver ions and reduce the silver ions to nanoparticles. The carboxylic groups in the extract may enhance the stability of silver nanoparticles.Figure 8
FTIR shows the functional groups associated with silver nanoparticles synthesized from extract ofS. longifolium.


### 3.7. Antifungal Activity of Algal Synthesized Silver Nanoparticles

Nanostructured silver materials can be initialized in some potential applications in the field of bactericidal activities and they vigorously involve the fight against the pathogenic microbes causing dangerous disease. The green synthesized silver nanoparticles exhibited excellent antibacterial activity against pathogenic fungi such asA. fumigatus, Candida albicans, and Fusarium sp. Silver could be used as an antibacterial agent for many infectious diseases at ancient time and before the emergence of antibiotics [28]. In this study, the antifungal activity of S. longifoliummediated synthesized silver nanoparticles against harmful pathogenic fungi at different concentrations (50, 100, and 150 μL) was carried out. Table 1 shows that with the increase in the concentration of silver nanoparticles, the zone of inhibition increased. So the zone formation around the well is directly proportional to the concentration of silver nanoparticles (Figure 9). Silver nanoparticles highly form the zone against Fusariumsp. (22.03
±
0.033) mm at the concentration of 150 μL. Minimum zone inhibition was observed against A. fumigates(13.10
±
0.130) mm.Table 1
Antifungal activity of silver bionanoparticles against pathogenic fungi.
Pathogenic fungi
Zone of inhibition (mm in diameter)
50µL
100µL
150µL
Aspergillus fumigatus
11.17
±
0.152
12.07
±
0.120
13.10
±
0.130
Candida albicans
11.20
±
0.256
13.87
±
0.134
14.20
±
0.152
Fusarium sp.
18.10
±
0.208
20.23
±
0.186
22.03
±
0.033
±Standard deviation.Antifungal activity of SNPs synthesized by using marine algaeS. longifolium.
(a)
(b)
(c)The mechanism of antifungal activity of silver nanoparticles was not fully known. A few of the  literature illustrated the possible mechanism of antifungal activity. Antifungal activity depends on the size and shape of the silver nanoparticles. Small size nanoparticles have large surface area ensuring the inhibition of microbial growth. Spherical shape with size-reduced silver ions has the increased contact area so that it can eliminate the bacterial growth. Activity of silver nanoparticles has similar effects as silver ions [29]. Positively charged silver ions may attach with negatively charged cell membranes of microbes by electrostatic attraction [30]. Silver nanoparticles form the pits in the cell wall and damage the cell permeability [31] and induce the proton leakage caused by ROS in the membrane [32, 33] resulting in cell death. Kim et al. [34] demonstrated that silver nanoparticles inhibit the conidial germination on fungi. Finally, the silver nanoparticles have a great potential to control the spore producing fungi.


## 3.1. Visual and UV-Vis Spectrophotometer Analysis

Reduction of silver ions to silver nanoparticles was visually identified by color change from yellow to brown in the aqueous solution of reaction mixture at 1 hr incubation time (Figure1). Brown formation occurred due to the oscillation of free electrons in the reaction mixture. The color change depended on the incubation time. The deep brown color for silver nanoparticles was attained at 32 hr indicating that the increasing of color intensity is directly proportion to the time of incubation. Furthermore, the nanoparticles formation by the algal extract was confirmed by UV-vis spectroscopy at different wavelengths. Similarly, Chandran et al. [19] synthesized silver nanoparticles using Aloe vera extract taking 24 hr of reaction time in the presence of ammonia which enhances the nanoparticles formation.Figure 1
Color change after the addition of algae extract with 1 mM AgNO3 indicates the formation of silver nanoparticles. (a) Initial color change, (b) 1 hr, (c) 32 hr.UV-vis spectroscopy analysis depends on the arising of color in the reaction due to the excitation of surface Plasmon resonance band in a reaction mixture and was recorded as different functional time. Figure2 shows that, there is no peak were formed at the initial stage indicates that there is no synthesis of silver nanoparticles was observed.  After 1 hr of incubation time the surface Plasmon resonance band for silver nanoparticles was positioned at around 460 nm and the synthesis was steadily increasing with the increasing in time of reaction without change in the peak position. It shows no peak variation in their position with broad band indicates presence of polydispersed nanoparticles. The nanoparticles synthesis was completed at 64 h time of incubation.Figure 2
UV-vis spectra recorded the formation of nanoparticles in the reaction mixture of algae extract and AgNO3 at different time intervals showing the peak at 460 nm.


## 3.2. Effect of pH

The pH of the reaction mixture plays an important role in the nanoparticles synthesis. At low pH 6.2, the color change of the reaction mixture was slower than that at high pH 8.4. The color intensity of the reduction process increased with the increase of the pH (Figure3). Figure 4 shows the UV-vis spectra to analyze the pH impact on the nanoparticles synthesis. At lower pH 6.2 and 6.8, the broadened SPR band was shown at 460 nm indicating polydispersed nanoparticles formation. Broadening peaks occurred at lower pH due to the excitations of longitudinal Plasmon vibrations [20]. Broad band at low pH is due to the formation of anisotropic nanoparticles [21]. The narrow peak at higher pH that occurred is due to the formation of monodispersed and small-sized silver nanoparticles [22]. Lower pH suppresses nanoparticles formation and higher pH enhances the nanoparticles synthesis process. The high absorbance and narrow band was formed at 440 nm in the higher pH. Similarly, some reports explained that pH plays an important role in shape and size control synthesis process of silver nanoparticles. This is also in agreement with earlier reports that the addition of an alkaline ion in the reaction mixture is necessary to carry out the reduction of metal ions [23].Color change variation at different pH levels (a) pH 6.2 (b) 6.8 (c) 7.8 (d) 8.4.
(a)
(b)
(c)
(d)Figure 4
Effect of pH on the synthesis of silver nanoparticles shows that maximum synthesis occurred at high pH.


## 3.3. XRD Analysis

The crystalline structure of the silver nanoparticles was determined by XRD in the whole spectrum of 2θ values ranging from 10 to 80. The green synthesized silver nanostructure by employing algae extract of S. longifoliumwas confirmed by the characteristic peaks observed in the XRD image (Figure 5). The four distinct diffraction peaks of the 2θ values of 38.27°, 44.16°, 65.54°, and 77.42° could be assigned the plane of (1 1 1), (2 0 0), (2 2 0), and (3 1 1), respectively, indicating that the silver nanoparticles are fcc (face centered cubic) and crystalline in nature. The synthesized silver nanoparticles are compared with standard silver nitrate and pure silver particles which are published by Joint Committee on Powder Diffraction Standards (File nos. 04-0783 and 84-0713). The XRD clearly shows that the four distinct planes for the silver nanoparticles synthesized from algal extract of S. longifoliumare highly crystallized and purified.Figure 5
XRD spectrum of silver nanoparticles synthesized fromS. longifoliumextract shows crystalline structure of nanoparticles.


## 3.4. SEM Analysis

SEM image shows the morphologies of the algae mediated synthesized bionanoparticles at different magnifications (Figure6). High density of silver nanoparticles is shown in the SEM image which is uniformly distributed on the surface of the cells of algae. The polydispersed silver nanoparticles were attached on the surface of the biomolecules of the algal cells. Silver nanoparticles adhered on the cells due to weakly dislodging of bound silver nanoparticles from the biomass and silver nitrate reaction mixture during preparation [24]. Similar results were obtained in the gold nanoparticles using algal extract of S. marginatum with the size ranging from 40 to 85 nm [25] and also by using the T. conoides algae [26]. It reveals the involvement of the polyphenolic or secondary capping agent in the nanoparticles formation.Scanning electron microscope image of silver nanoparticles synthesized fromS. longifoliumextract showing predominantly spherical shape nanoparticles on the surface of the cell at different magnifications: (a) 5 μm, (b) 3 μm, (c) 1 μm, and (d) 500 nm.
(a)
(b)
(c)
(d)


## 3.5. TEM Image of Silver Nanoparticles

The morphology ofS. longifoliumalgae extract mediated synthesized silver nanoparticles was determined by TEM. The synthesized silver nanoparticles were formed predominantly spherical and some are truncated and ellipsoidal in the form of agglomerates. Some of the nanoparticles noted that the edges of the particles were smoother than the centers (marked by arrows) in Figure 7. The edges of nanoparticles were shown smoother than the center indicating that the proteins in algal extract may cap the silver nanoparticles. Similarly, Ahmad et al. [27] suggested that proteins in basil plant are present among the particles and they adhered to their surfaces.Figure 7
TEM image of silver nanoparticles shows polydispersed synthesized silver nanoparticles.


## 3.6. FTIR

FTIR was used to identify the biomolecules inS. longifoliumresponsible for the silver ions reduction and stabilization of reduced silver ions (Figure 8). The broad spectrum at 3382 cm−1 shows the O–H bond stretching of alcohols and phenols. The narrow band at 1639 cm−1 (–NH–C=O) indicates the presence of N–H bended primary amines. A weak band at 1386 cm−1 corresponds to asymmetrical stretching for nitro compounds. The absorption peak at 1033 cm−1 illustrates the presence of carboxylic acid functional group. The very weak bands were formed at 613 and 504 cm−1 due to the occurrence of vibrations of alkyl halides. FTIR revealed that soluble organic compounds or proteins in the extract may bind with silver ions and reduce the silver ions to nanoparticles. The carboxylic groups in the extract may enhance the stability of silver nanoparticles.Figure 8
FTIR shows the functional groups associated with silver nanoparticles synthesized from extract ofS. longifolium.


## 3.7. Antifungal Activity of Algal Synthesized Silver Nanoparticles

Nanostructured silver materials can be initialized in some potential applications in the field of bactericidal activities and they vigorously involve the fight against the pathogenic microbes causing dangerous disease. The green synthesized silver nanoparticles exhibited excellent antibacterial activity against pathogenic fungi such asA. fumigatus, Candida albicans, and Fusarium sp. Silver could be used as an antibacterial agent for many infectious diseases at ancient time and before the emergence of antibiotics [28]. In this study, the antifungal activity of S. longifoliummediated synthesized silver nanoparticles against harmful pathogenic fungi at different concentrations (50, 100, and 150 μL) was carried out. Table 1 shows that with the increase in the concentration of silver nanoparticles, the zone of inhibition increased. So the zone formation around the well is directly proportional to the concentration of silver nanoparticles (Figure 9). Silver nanoparticles highly form the zone against Fusariumsp. (22.03
±
0.033) mm at the concentration of 150 μL. Minimum zone inhibition was observed against A. fumigates(13.10
±
0.130) mm.Table 1
Antifungal activity of silver bionanoparticles against pathogenic fungi.
Pathogenic fungi
Zone of inhibition (mm in diameter)
50µL
100µL
150µL
Aspergillus fumigatus
11.17
±
0.152
12.07
±
0.120
13.10
±
0.130
Candida albicans
11.20
±
0.256
13.87
±
0.134
14.20
±
0.152
Fusarium sp.
18.10
±
0.208
20.23
±
0.186
22.03
±
0.033
±Standard deviation.Antifungal activity of SNPs synthesized by using marine algaeS. longifolium.
(a)
(b)
(c)The mechanism of antifungal activity of silver nanoparticles was not fully known. A few of the  literature illustrated the possible mechanism of antifungal activity. Antifungal activity depends on the size and shape of the silver nanoparticles. Small size nanoparticles have large surface area ensuring the inhibition of microbial growth. Spherical shape with size-reduced silver ions has the increased contact area so that it can eliminate the bacterial growth. Activity of silver nanoparticles has similar effects as silver ions [29]. Positively charged silver ions may attach with negatively charged cell membranes of microbes by electrostatic attraction [30]. Silver nanoparticles form the pits in the cell wall and damage the cell permeability [31] and induce the proton leakage caused by ROS in the membrane [32, 33] resulting in cell death. Kim et al. [34] demonstrated that silver nanoparticles inhibit the conidial germination on fungi. Finally, the silver nanoparticles have a great potential to control the spore producing fungi.


## 4. Conclusion

In conclusion, this present study stated the green mediated synthesis of silver nanoparticles under optimized parameters using the extract of enormously available algaeS. longifolium. The broad peak was observed under UV-vis spectra at 460 nm for silver nanoparticles. Highly synthesis and spherical shape nanoparticles were obtained at higher pH confirmed by TEM. Crystalline structure of nanoparticles was identified by XRD. Purity and component of silver nanoparticles were confirmed by EDX. S. longifoliumextract mediated synthesized silver nanoparticles show high antifungal activity that can be used therapeutically in biomedical applications.



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*Source: 692643-2014-01-19.xml*