# Synthesis, Characterization and Evaluation of the Cytotoxicity of Ni-Doped Zn(Se,S) Quantum Dots

Authors: Melissa Cruz-Acuña; Sonia Bailón-Ruiz; Carlos R. Marti-Figueroa; Ricardo Cruz-Acuña; Oscar J. Perales-Pérez
Journal: Journal of Nanomaterials
 (2015)

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## Abstract

Quantum dots (QDs) are semiconductor nanocrystals with desirable optical properties for biological applications, such as bioimaging and drug delivery. However, the potential toxicity of these nanostructures in biological systems limits their application. The present work is focused on the synthesis, characterization, and evaluation of the toxicity of water-stable Ni-doped Zn(Se,S) QDs. Also, the study of nondoped nanostructures was included for comparison purposes. Ni-doped nanostructures were produced from zinc chloride and selenide aqueous solutions in presence of 3-mercaptopropionic acid and Ni molar concentration of 0.001 M. In order to evaluate the potential cytoxicity of these doped nanostructures, human pancreatic carcinoma cells (PANC-1) were used as model. The cell viability was monitored in presence of Ni-doped Zn(Se,S) QDs at concentrations ranging from 0μg/mL to 500 μg/mL and light excited Ni-doped Zn(Se,S) nanostructures were evaluated at 50 μg/mL. Results suggested that Ni-doped Zn(Se,S) nanostructures were completely safe to PANC-1 when concentrations from 0 μg/mL to 500 μg/mL were used, whereas non-doped nanostructures evidenced toxicity at concentrations higher than 200 μg/mL. Also, Ni-doped Zn(Se,S) QDs under light excitation do not evidence toxicity to PANC-1. These findings suggest strongly that Zn(Se,S) nanostructures doped with nickel could be used in a safe manner in light-driving biological applications and drug delivery.


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## Body

## 1. Introduction

Quantum dots (QDs) are semiconductor nanocrystals that exhibit tunable emission, high absorption coefficients and exceptional chemical and photostability [1–5]. Their corresponding optical properties can be tuned by altering their size, composition, and crystal array, and, moreover, make them suitable for biological purposes such as drug delivery and bio-imaging [6–8].Traceable drug delivery is one of the most important applications of QDs because it provides the possibility of studying the pharmacokinetics and pharmacodynamics of potential drugs. In this way, nontoxic and photochemically stable QDs are needed in order to facilitate their functionalization and traceability by fluorescence [9].Cd-based chalcogenides are the most studied quantum dots. However, there is a prevailing concern about the potential long-term toxicity they could provoke to biological systems, which limits their application for in vitro or small animal studies [10, 11]. For this reason, Zn-based materials are attractive substitutes that have no documented toxicity and still exhibit useful optical and chemical properties. Studies have reported that adding doping agents in trace concentrations to these Zn-based nanostructures is possible to change their corresponding optical properties, increase the quantum yield, and also reduce or increase the emission traps [12, 13]. Nickel (II) is a transition metal that represents an option as dopant agent for Zn-based QDs due to its atomic radius, which differs within the 10% limit (8.3% of difference) to Zn2+ radius. Therefore, the substitution of Zn2+ atoms by Ni2+ atoms in the lattice is possible.Frequently, QDs are synthesized in organic phase under refluxing, using coordinating solvents like trioctylphosphine oxide (TOPO), trioctylphosphine (TOP), and hexadecylamine [14–20]. Although, the reflux technique is highly reproducible and able to produce nanocrystals of narrow size distribution; the long time period required for the synthesis; the limited temperatures that can be achieved by this technique; and the generation of QDs stable in organic media limit its application. Furthermore, QDs stable in organic solvent are required for a ligand-exchange procedure in order to stabilize them in aqueous phase and can be used in biological applications [17, 18].The Microwave irradiation technique has been recently used to produce nanoparticles because of its high reproducibility, less cost of energy and homogeneous heating capability which promote the generation of well monodispersed fluorescent nanoparticles. The possibility to produce water-stable nanoparticles using thiols agents under microwave irradiation is desirable to biomedical applications [19–24]. In this study, we have produced water-stable Ni-doped Zn(Se,S) nanocrystals of high-optical quality using 3-mercaptopropionic acid (MPA) under microwave irradiation. Ni-doped nanostructures characterized morphological, structurally and optically; were used in cytotoxicity studies using Human Pancreatic Carcinoma Cells (PANC-1) as model. Nondoped nanostructures also were used as comparison purposes.


## 2. Material and Methods

### 2.1. Synthesis of Ni-Doped Zn(Se,S) Quantum Dots

Ni-doped nanostructures were synthesized from initial zinc chloride (99.999% trace metal basis), selenide and nickel chloride solutions in presence of 3-mercaptopropionic acid (MPA, 99.0%) in a CEM Mars Xpress digestion system operated at 200 W. All reactants were supply by Sigma-Aldrich. The selenide solution was prepared previously through the reduction of elemental selenium powder (99.99% trace metal basis) with sodium bisulfide (>98%). The molar ratio selenium/sodium bisulfide was 1.0 M/5.0 M and the reduction was carried out at 100°C during 8 hours. Ni-doped Nanoparticles were prepared as follow: Zinc chloride solution was mixed with MPA in order to form a stable complex and to avoid the formation of zinc hydroxides. Then, nickel solution was added to the solution in order to attain molar concentrations from 0.0005 M to 0.010 M. Finally, reduced selenide solution was added and the mixed solution was put into the vessel and heated using the microwave. The final molar ratio Zn/MPA/Se on all synthesis was estimated to be 1/2.4/0.04. Also, for comparison purposes, nondoped nanostructures were synthesized using the same molar ratios Zn/MPA/Se. The microwave power was set on 200 W, pre-heating at 50°C for 15 minutes, increasing the temperature from 50 to 140°C for 6 minutes, and the actual reaction stage took place at 140°C during 30 minutes. Once the reaction concluded, the solution was cooled to room temperature, coagulated using 2-propanol, centrifuged, and resuspended in deionized water.


### 2.2. Characterization Techniques

A high-resolution TEM (HRTEM) analysis was made on a JEM-ARM200F and accomplished at 200 KV. A Shimadtzu 1800 UV/Vis Spectrophotometer was used to obtain UV-Vis absorption measurements. The photoluminescence (PL) spectra were obtained using a Shimadtzu RF-5301 Spectrofluorometer with a 150 W Xenon lamp as excitation source. All measurements were performed at room temperature. With the purpose to study the desirable photostability of QDs needed to biological applications, nanostructures were irradiated under 302 nm during 60 minutes using a UV lamp with a power density of 10 mW cm−2.


### 2.3. Cell Culture

Human pancreatic carcinoma cells (PANC-1) were purchased from ATCC (CRL number 1469) and cultured in DMEM (Dulbecco’s Modified Eagle Medium) containing 10% (v/v) bovine fetal serum and 50µg/L gentamicin at temperature of 37°C under humidified air containing 5% CO2.


### 2.4. Cytotoxicity Assays

The cell viability of PANC-1 cells exposed to Ni-doped nanostructures was determined by using a T4 Automatic Cell Counter. PANC-1 cells were cultured in a CellBind 25 cm2 flask until it was observed 80% of confluence. Cells were trypsinized, homogenized, added to 96-well plate and waited until cells attached. Ni-doped nanostructures were re-suspended in PBS and added to each well to achieve final concentrations ranging from 0 to 500 µg/mL. The incubation time was 48 hours. Finally, cells were trypsinized and mixed with trypan blue to measure the corresponding cell viability.The viability of PANC-1 cells under light-irradiated Ni-doped nanostructures was investigated. Ni-doped nanostructures dispersed in PBS were added to PANC-1 cells that were seeded in 48-well plates in order to achieve a final concentration of 50µg/mL. Cells in presence of Ni-doped nanostructures were irradiated using a 302 nm-UV lamp (8 watts, power density of 10 mW cm−2) up to 60 minutes. The cell viability was assessed after 4 hours of post-incubation at 37°C in humidified air containing 5% CO2.Nondoped nanostructures (ZnSeS QDs) and control samples (only cells) were evaluated in the same conditions to Ni-doped nanostructures as comparison purposes.


## 2.1. Synthesis of Ni-Doped Zn(Se,S) Quantum Dots

Ni-doped nanostructures were synthesized from initial zinc chloride (99.999% trace metal basis), selenide and nickel chloride solutions in presence of 3-mercaptopropionic acid (MPA, 99.0%) in a CEM Mars Xpress digestion system operated at 200 W. All reactants were supply by Sigma-Aldrich. The selenide solution was prepared previously through the reduction of elemental selenium powder (99.99% trace metal basis) with sodium bisulfide (>98%). The molar ratio selenium/sodium bisulfide was 1.0 M/5.0 M and the reduction was carried out at 100°C during 8 hours. Ni-doped Nanoparticles were prepared as follow: Zinc chloride solution was mixed with MPA in order to form a stable complex and to avoid the formation of zinc hydroxides. Then, nickel solution was added to the solution in order to attain molar concentrations from 0.0005 M to 0.010 M. Finally, reduced selenide solution was added and the mixed solution was put into the vessel and heated using the microwave. The final molar ratio Zn/MPA/Se on all synthesis was estimated to be 1/2.4/0.04. Also, for comparison purposes, nondoped nanostructures were synthesized using the same molar ratios Zn/MPA/Se. The microwave power was set on 200 W, pre-heating at 50°C for 15 minutes, increasing the temperature from 50 to 140°C for 6 minutes, and the actual reaction stage took place at 140°C during 30 minutes. Once the reaction concluded, the solution was cooled to room temperature, coagulated using 2-propanol, centrifuged, and resuspended in deionized water.


## 2.2. Characterization Techniques

A high-resolution TEM (HRTEM) analysis was made on a JEM-ARM200F and accomplished at 200 KV. A Shimadtzu 1800 UV/Vis Spectrophotometer was used to obtain UV-Vis absorption measurements. The photoluminescence (PL) spectra were obtained using a Shimadtzu RF-5301 Spectrofluorometer with a 150 W Xenon lamp as excitation source. All measurements were performed at room temperature. With the purpose to study the desirable photostability of QDs needed to biological applications, nanostructures were irradiated under 302 nm during 60 minutes using a UV lamp with a power density of 10 mW cm−2.


## 2.3. Cell Culture

Human pancreatic carcinoma cells (PANC-1) were purchased from ATCC (CRL number 1469) and cultured in DMEM (Dulbecco’s Modified Eagle Medium) containing 10% (v/v) bovine fetal serum and 50µg/L gentamicin at temperature of 37°C under humidified air containing 5% CO2.


## 2.4. Cytotoxicity Assays

The cell viability of PANC-1 cells exposed to Ni-doped nanostructures was determined by using a T4 Automatic Cell Counter. PANC-1 cells were cultured in a CellBind 25 cm2 flask until it was observed 80% of confluence. Cells were trypsinized, homogenized, added to 96-well plate and waited until cells attached. Ni-doped nanostructures were re-suspended in PBS and added to each well to achieve final concentrations ranging from 0 to 500 µg/mL. The incubation time was 48 hours. Finally, cells were trypsinized and mixed with trypan blue to measure the corresponding cell viability.The viability of PANC-1 cells under light-irradiated Ni-doped nanostructures was investigated. Ni-doped nanostructures dispersed in PBS were added to PANC-1 cells that were seeded in 48-well plates in order to achieve a final concentration of 50µg/mL. Cells in presence of Ni-doped nanostructures were irradiated using a 302 nm-UV lamp (8 watts, power density of 10 mW cm−2) up to 60 minutes. The cell viability was assessed after 4 hours of post-incubation at 37°C in humidified air containing 5% CO2.Nondoped nanostructures (ZnSeS QDs) and control samples (only cells) were evaluated in the same conditions to Ni-doped nanostructures as comparison purposes.


## 3. Results and Discussion

### 3.1. Structural and Morphological Characterization

X-ray Diffraction (XRD) patterns of synthesized nondoped and Ni-doped nanostructures suggest the formation of zinc blende structures (Figure1). The presence of the diffraction peaks between those 2θ angles corresponding to bulk ZnSe (JCPDS card 80–0021) and ZnS (JCPDS card 77–2100) indicates the development of a solid solution of the type Zn(Se,S) for nondoped and Ni-doped nanostructures. The presence of nickel in trace concentration (0.001 M) into the lattice does not change the original crystalline structure type blenda (Figure 1(b)). The particle sizes for nondoped and Ni-doped nanostructures were of 2.1 ± 0.2 nm and 2.8 ± 0.3 nm, respectively, which were estimated from X-ray line broadening using the Debye-Scherrer’s relationship. The slight increase of crystal size observed in Ni-doped nanostructures is according to observations found by Cong et al., where Zn-based nanoparticles increase its size in presence of nickel as dopant agent [25]. The ionic radius of Ni2+ (0.55 Å) is slightly smaller than that of the Zn2+ (0.60 Å) ion in tetrahedral coordination, which suggests the factible substitution of the Zn2+ ion by the Ni2+ and it is incorporation into the lattice structure [25]. The lattice parameter was estimated to be 0.539 and 0.556, for Ni-doped nanostructures and nondoped nanostructures respectively, which falls in-between the values for bulk ZnSe (0.567 nm) and ZnS (0.538 nm). The estimated values of the lattice parameters confirm the generation of ternary solid solutions of the Zn(S,Se) type where the incorporation of Ni species did not affect the structural array.Figure 1
X-ray diffraction spectrum for nondoped Zn(Se,S) QDs (a) and 0.001 M Ni-doped Zn(Se,S) QDs (b) synthesized in presence of MPA at 140°C. Lines correspond to the position of ZnSe (top) and ZnS (bottom) bulk peaks.The presence of nickel into the synthesized samples was corroborated by ICP-MS. The Zn/Ni molar ratios in Ni-doped nanostructures were 44 ± 3. Also, Molar ratios of Zn/Se for Ni-doped nanostructures were 3.3 ± 0.2.The small size under 4 nm of the synthesized nanostructures was corroborated with the HRTEM images (Figures2 and 3). The images evidence that nondoped and Ni-doped nanostructures were crystallines. The electron diffractions patterns for Figure 2 (bottom) and Figure 3 (rigth) also, show the (111), (220), (311) planes that affirm the cubic Zn (Se,S) structure array in these synthesized nanostructures.HRTEM of Ni-doped Zn(Se,S) quantum dots synthesized in presence of MPA (top) and ED pattern (bottom).
(a)
(b)HRTEM of nondoped Zn(Se,S) quantum dots synthesized in presence of MPA (left) and ED pattern (right).
(a)
(b)The generation of the nanostructures can be explained by initial fast reaction between Zn and selenide species, which forms the ZnSe nanostructures. In this way, some zinc ions should be replaced by nickel ions. Then, the sulfide species formed by the thermal decomposition of MPA, enter into the ZnSe lattice forming a solid solution of Zn (Se,S). Subsequently, the adsorption of nonreacted thiol species by the surface of the nanostructures, would be establishing a net negative surface charge that prevents the nanocrystals from aggregation [19, 24]. The presence of MPA forming a bridging bidentate complex with zinc ions on the Ni-doped QDs surface was confirmed by FT-IR (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/702391).


### 3.2. Optical Properties

Figure4 corresponds to the UV-Vis absorption spectra for Ni-doped Zn(Se,S) QDs synthesized in presence of MPA at nickel molar concentrations ranged from 0.0005 M to 0.010 M. There was not detected a remarkable effect of the nickel concentrations on the position of the absorption peak (~373 nm); therefore, a minimum variation in the nanocrystals size can be suggested. The estimated band gap energy values were 3.1 eV for all samples synthesized in presence of Ni molar concentrations from 0.0005 M to 0.010 M. The estimated band gap values higher than 2.7 eV (bulk) suggest a quantum confinement effect in the synthesized samples.Figure 4
UV-Vis absorption spectra of Ni-doped Zn(Se,S) QDs at various molar concentrations of nickel.Figure5 corresponds to the photoluminescence spectra of Ni-doped Zn(Se,S) nanostructures synthesized in presence of MPA at nickel molar concentrations ranged from 0.0005 M to 0.010 M. A main PL peak centered at ~400 nm and a broad peak at 470 nm for all doped samples are evident in these results. Samples synthesized at 0.001 M nickel showed the highest PL intensity at ~400 nm and a quenching in the photoluminescence was observed when nickel concentrations higher than 0.001 M were used. This drop in the luminescence intensity can be a consequence of the preferential formation of NiS. Previous reports have evidenced that NiS should act as nonradiative recombination centers and reduce the number of Ni+2 ions available that are optically active luminescence centers in doped nanocrystals [26].Figure 5
PL emission spectra of Ni-doped Zn(Se,S) QDs at various molar concentrations of nickel. The excitation wavelength was 311 nm.Figure6 shows the UV-Vis (left) and PL (right) spectra for nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs synthesized at 140°C using MPA. The slightly red shift (~373 nm) observed in the UV-Vis absorption spectra for Ni-doped Zn(Se,S) QDs in comparison to the nondoped Zn(Se,S) QDs (~348 nm) suggests that Ni-doped Zn(Se,S) QDs have a size slightly higher than nondoped Zn(Se,S) QDs. The band gaps for each sample estimated according to the Tauc’s relation were 3.3 and 3.1 eV for nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs, respectively [27]. These results are in according to Cong et al., where they observed the decrease in the band gap of nanostructures doped with nickel [25].Figure 6
UV-Vis absorption (left) and PL emission (right) spectra Zn(Se,S) QDs (a) and Ni-doped Zn(Se,S) QDs (b). The excitation wavelength was 311 nm.The position of the emission bands (Figure6-right) is very similar (~400) for pure and doped QDs (Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs synthesized in presence of MPA). However, the broad band at 470 nm attributed to emission trap was significantly reduced in presence of nickel. In this way, the variation of the main/trap emission intensities ratios for pure QDs and 0.001 M Ni-doped QDs were 1.5 and 3.5, respectively. These values suggested that the nickel in adequate concentrations enhances the intensity of the band gap emission peak (~400 nm) and decreases the intensity of the emission traps (~470 nm).


### 3.3. Photostability Assays

An essential consideration to extend the use of QDs to biological applications is that they can maintain a stable luminescence under light excitation. The variation of the intensity of the main emission peak was evaluated when nondoped and Ni-doped Zn(Se,S) QDs were subjected to UV (302 nm)-irradiation at different time points. The observed luminescence fluctuation should be an indicative of the photostability of produced nanocrystals.Figure7 shows the variation of the normalized emission intensity [(intensity after UV exposure/initial intensity in water) × 100] of the QDs as a function of the UV-irradiation time. QDs were suspended in water or PBS and UV-irradiated for: 0 min (control test), 15 min, 30 min or 60 min using a lamp with a power density of 10 mW cm−2. The normalized main emission peak intensity for the control test of QDs suspended in deionized water was 98% for nondoped Zn(Se,S) QDs and 99% for Ni-doped Zn(Se,S) QDs. QDs re-suspended in PBS achieved normalized emission intensities of 93 ± 3% and 85 ± 2%, for nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs respectively, at 0 minutes of UV (302 nm)-irradiation. These results demonstrated excellent stabilities of nondoped and Ni-doped QDs in either deionized water or PBS.Figure 7
Normalized photoluminescence of nondoped Zn(Se,S) and Ni-doped Zn(Se,S) QDs in water or PBS, after UV (302 nm)-irradiation.A notable increase in the normalized emission intensity after 60 min of UV irradiation was observed on nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs suspended in both, water or PBS, compared to the control test. The intensity increased 88% on nondoped Zn(Se,S) QDs and 23% on Ni-doped Zn(Se,S) QDs suspended in water after 60 minutes of UV-irradiation. Also, the PL intensities for the same QDs resuspended in PBS, were enhanced in 153% and 29%, respectively. It is believed that this photoinduced enhancement in the PL should be associated to the “oxygen passivation” of the nanocrystals surface [21]. The presence of these species on the nanostructure’s surface should passivate the surface states that are not coated by MPA, promoting the increase on the photoluminescence of the QD [21]. The latter mentioned results demonstrated a great photostability of nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs under UV irradiation, an important consideration for biological applications.


### 3.4. Intrinsic Cytotoxicity of Ni-Doped Nanostructures

The data shown in Figure8 revealed that Ni-doped Zn(Se,S) QDs were completely safe to PANC-1 cells. The viability of the PANC-1 exposed to Ni-doped Zn(Se,S) QDs was nearly 100% at 500 μg/mL of concentration. Contrarily, nondoped Zn(Se,S) QDs started to be toxic, affecting significantly the viability of the cells, at 200 μg/mL and higher concentrations (p
<
0.01). The cell viability of nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs at 200 μg/mL were 80 ± 7% and 98 ± 4%, respectively.Figure 8
Viability percent of cells exposed to QDs at 0 to 500µg/mL after 48 hours of exposure.The observed toxicity for nondoped Zn(Se,S) QDs could suggest the production of reactive oxygen species in biological media. Li et al. [28] have suggested the promoted generation of cytotoxic reactive oxygen species or free radicals (ROS) depending on the surface features of the nanocrystals [28]. It has been previously demonstrated that certain types of QDs could produce ROS even without light excitation [29]. Also, it had been reported that the production free radicals is promoted by the generation of the pair electron-hole (exciton) and migration of this exciton to the quantum dots surface in order to reduce the oxygen and generate the radical peroxide or oxidize the water and produce the OH radical. If the exciton is recombined before its migration to the quantum dots surface, the generation of free radicals does not happen. In this way, the presence of nickel in the quantum dots structure should promote the recombination of the pair electron-hole before their interaction with surface species like oxygen or water. As a consequence the presence of nickel avoided the production of ROS.


### 3.5. Cytotoxicity of Ni-Doped Nanostructures Activated by Light

The cytotoxicity of nondoped Zn(Se,S) QDs and Ni-doped ZnSeS QDs at concentrations of 50µg/mL was evaluated on PANC-1 at different intervals of UV-exposure (Figure 9). The PANC-1 viability was not affected by the presence of UV-irradiated Ni-doped Zn(Se,S) QDs after 60 minutes of UV irradiation. The viability of the PANC-1 exposed to Ni-doped Zn(Se,S) QDs was 92 ± 3% after a period of 60 minutes of UV irradiation. The cell viability for the control test (only cells irradiated with UV during 60 minutes) was 90 ± 3%. In this way, the cell viability for nondoped Zn(Se,S) QDS decreases as much as 36 ± 4% after 60 min under UV-irradiation. The observed increase in cytotoxicity for nondoped Zn(Se,S) QDs could be explained by the production of toxic species like singlet oxygen and ROS induced by the UV excitation. UV-irradiated Ni-doped Nanostructures at high concentration (500 μg/mL) were also evaluated, evidenced absence of toxicity to PANC-1.Figure 9
Effect of UV irradiation time on PANC-1 viability. The final concentration of the nondoped and Ni-doped Zn(Se,S) QDs was 50μg/mL. Blanks were cells incubated with no QDs. Data showed the mean value ± SD of three independent experiments (n
=
3).


## 3.1. Structural and Morphological Characterization

X-ray Diffraction (XRD) patterns of synthesized nondoped and Ni-doped nanostructures suggest the formation of zinc blende structures (Figure1). The presence of the diffraction peaks between those 2θ angles corresponding to bulk ZnSe (JCPDS card 80–0021) and ZnS (JCPDS card 77–2100) indicates the development of a solid solution of the type Zn(Se,S) for nondoped and Ni-doped nanostructures. The presence of nickel in trace concentration (0.001 M) into the lattice does not change the original crystalline structure type blenda (Figure 1(b)). The particle sizes for nondoped and Ni-doped nanostructures were of 2.1 ± 0.2 nm and 2.8 ± 0.3 nm, respectively, which were estimated from X-ray line broadening using the Debye-Scherrer’s relationship. The slight increase of crystal size observed in Ni-doped nanostructures is according to observations found by Cong et al., where Zn-based nanoparticles increase its size in presence of nickel as dopant agent [25]. The ionic radius of Ni2+ (0.55 Å) is slightly smaller than that of the Zn2+ (0.60 Å) ion in tetrahedral coordination, which suggests the factible substitution of the Zn2+ ion by the Ni2+ and it is incorporation into the lattice structure [25]. The lattice parameter was estimated to be 0.539 and 0.556, for Ni-doped nanostructures and nondoped nanostructures respectively, which falls in-between the values for bulk ZnSe (0.567 nm) and ZnS (0.538 nm). The estimated values of the lattice parameters confirm the generation of ternary solid solutions of the Zn(S,Se) type where the incorporation of Ni species did not affect the structural array.Figure 1
X-ray diffraction spectrum for nondoped Zn(Se,S) QDs (a) and 0.001 M Ni-doped Zn(Se,S) QDs (b) synthesized in presence of MPA at 140°C. Lines correspond to the position of ZnSe (top) and ZnS (bottom) bulk peaks.The presence of nickel into the synthesized samples was corroborated by ICP-MS. The Zn/Ni molar ratios in Ni-doped nanostructures were 44 ± 3. Also, Molar ratios of Zn/Se for Ni-doped nanostructures were 3.3 ± 0.2.The small size under 4 nm of the synthesized nanostructures was corroborated with the HRTEM images (Figures2 and 3). The images evidence that nondoped and Ni-doped nanostructures were crystallines. The electron diffractions patterns for Figure 2 (bottom) and Figure 3 (rigth) also, show the (111), (220), (311) planes that affirm the cubic Zn (Se,S) structure array in these synthesized nanostructures.HRTEM of Ni-doped Zn(Se,S) quantum dots synthesized in presence of MPA (top) and ED pattern (bottom).
(a)
(b)HRTEM of nondoped Zn(Se,S) quantum dots synthesized in presence of MPA (left) and ED pattern (right).
(a)
(b)The generation of the nanostructures can be explained by initial fast reaction between Zn and selenide species, which forms the ZnSe nanostructures. In this way, some zinc ions should be replaced by nickel ions. Then, the sulfide species formed by the thermal decomposition of MPA, enter into the ZnSe lattice forming a solid solution of Zn (Se,S). Subsequently, the adsorption of nonreacted thiol species by the surface of the nanostructures, would be establishing a net negative surface charge that prevents the nanocrystals from aggregation [19, 24]. The presence of MPA forming a bridging bidentate complex with zinc ions on the Ni-doped QDs surface was confirmed by FT-IR (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/702391).


## 3.2. Optical Properties

Figure4 corresponds to the UV-Vis absorption spectra for Ni-doped Zn(Se,S) QDs synthesized in presence of MPA at nickel molar concentrations ranged from 0.0005 M to 0.010 M. There was not detected a remarkable effect of the nickel concentrations on the position of the absorption peak (~373 nm); therefore, a minimum variation in the nanocrystals size can be suggested. The estimated band gap energy values were 3.1 eV for all samples synthesized in presence of Ni molar concentrations from 0.0005 M to 0.010 M. The estimated band gap values higher than 2.7 eV (bulk) suggest a quantum confinement effect in the synthesized samples.Figure 4
UV-Vis absorption spectra of Ni-doped Zn(Se,S) QDs at various molar concentrations of nickel.Figure5 corresponds to the photoluminescence spectra of Ni-doped Zn(Se,S) nanostructures synthesized in presence of MPA at nickel molar concentrations ranged from 0.0005 M to 0.010 M. A main PL peak centered at ~400 nm and a broad peak at 470 nm for all doped samples are evident in these results. Samples synthesized at 0.001 M nickel showed the highest PL intensity at ~400 nm and a quenching in the photoluminescence was observed when nickel concentrations higher than 0.001 M were used. This drop in the luminescence intensity can be a consequence of the preferential formation of NiS. Previous reports have evidenced that NiS should act as nonradiative recombination centers and reduce the number of Ni+2 ions available that are optically active luminescence centers in doped nanocrystals [26].Figure 5
PL emission spectra of Ni-doped Zn(Se,S) QDs at various molar concentrations of nickel. The excitation wavelength was 311 nm.Figure6 shows the UV-Vis (left) and PL (right) spectra for nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs synthesized at 140°C using MPA. The slightly red shift (~373 nm) observed in the UV-Vis absorption spectra for Ni-doped Zn(Se,S) QDs in comparison to the nondoped Zn(Se,S) QDs (~348 nm) suggests that Ni-doped Zn(Se,S) QDs have a size slightly higher than nondoped Zn(Se,S) QDs. The band gaps for each sample estimated according to the Tauc’s relation were 3.3 and 3.1 eV for nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs, respectively [27]. These results are in according to Cong et al., where they observed the decrease in the band gap of nanostructures doped with nickel [25].Figure 6
UV-Vis absorption (left) and PL emission (right) spectra Zn(Se,S) QDs (a) and Ni-doped Zn(Se,S) QDs (b). The excitation wavelength was 311 nm.The position of the emission bands (Figure6-right) is very similar (~400) for pure and doped QDs (Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs synthesized in presence of MPA). However, the broad band at 470 nm attributed to emission trap was significantly reduced in presence of nickel. In this way, the variation of the main/trap emission intensities ratios for pure QDs and 0.001 M Ni-doped QDs were 1.5 and 3.5, respectively. These values suggested that the nickel in adequate concentrations enhances the intensity of the band gap emission peak (~400 nm) and decreases the intensity of the emission traps (~470 nm).


## 3.3. Photostability Assays

An essential consideration to extend the use of QDs to biological applications is that they can maintain a stable luminescence under light excitation. The variation of the intensity of the main emission peak was evaluated when nondoped and Ni-doped Zn(Se,S) QDs were subjected to UV (302 nm)-irradiation at different time points. The observed luminescence fluctuation should be an indicative of the photostability of produced nanocrystals.Figure7 shows the variation of the normalized emission intensity [(intensity after UV exposure/initial intensity in water) × 100] of the QDs as a function of the UV-irradiation time. QDs were suspended in water or PBS and UV-irradiated for: 0 min (control test), 15 min, 30 min or 60 min using a lamp with a power density of 10 mW cm−2. The normalized main emission peak intensity for the control test of QDs suspended in deionized water was 98% for nondoped Zn(Se,S) QDs and 99% for Ni-doped Zn(Se,S) QDs. QDs re-suspended in PBS achieved normalized emission intensities of 93 ± 3% and 85 ± 2%, for nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs respectively, at 0 minutes of UV (302 nm)-irradiation. These results demonstrated excellent stabilities of nondoped and Ni-doped QDs in either deionized water or PBS.Figure 7
Normalized photoluminescence of nondoped Zn(Se,S) and Ni-doped Zn(Se,S) QDs in water or PBS, after UV (302 nm)-irradiation.A notable increase in the normalized emission intensity after 60 min of UV irradiation was observed on nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs suspended in both, water or PBS, compared to the control test. The intensity increased 88% on nondoped Zn(Se,S) QDs and 23% on Ni-doped Zn(Se,S) QDs suspended in water after 60 minutes of UV-irradiation. Also, the PL intensities for the same QDs resuspended in PBS, were enhanced in 153% and 29%, respectively. It is believed that this photoinduced enhancement in the PL should be associated to the “oxygen passivation” of the nanocrystals surface [21]. The presence of these species on the nanostructure’s surface should passivate the surface states that are not coated by MPA, promoting the increase on the photoluminescence of the QD [21]. The latter mentioned results demonstrated a great photostability of nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs under UV irradiation, an important consideration for biological applications.


## 3.4. Intrinsic Cytotoxicity of Ni-Doped Nanostructures

The data shown in Figure8 revealed that Ni-doped Zn(Se,S) QDs were completely safe to PANC-1 cells. The viability of the PANC-1 exposed to Ni-doped Zn(Se,S) QDs was nearly 100% at 500 μg/mL of concentration. Contrarily, nondoped Zn(Se,S) QDs started to be toxic, affecting significantly the viability of the cells, at 200 μg/mL and higher concentrations (p
<
0.01). The cell viability of nondoped Zn(Se,S) QDs and Ni-doped Zn(Se,S) QDs at 200 μg/mL were 80 ± 7% and 98 ± 4%, respectively.Figure 8
Viability percent of cells exposed to QDs at 0 to 500µg/mL after 48 hours of exposure.The observed toxicity for nondoped Zn(Se,S) QDs could suggest the production of reactive oxygen species in biological media. Li et al. [28] have suggested the promoted generation of cytotoxic reactive oxygen species or free radicals (ROS) depending on the surface features of the nanocrystals [28]. It has been previously demonstrated that certain types of QDs could produce ROS even without light excitation [29]. Also, it had been reported that the production free radicals is promoted by the generation of the pair electron-hole (exciton) and migration of this exciton to the quantum dots surface in order to reduce the oxygen and generate the radical peroxide or oxidize the water and produce the OH radical. If the exciton is recombined before its migration to the quantum dots surface, the generation of free radicals does not happen. In this way, the presence of nickel in the quantum dots structure should promote the recombination of the pair electron-hole before their interaction with surface species like oxygen or water. As a consequence the presence of nickel avoided the production of ROS.


## 3.5. Cytotoxicity of Ni-Doped Nanostructures Activated by Light

The cytotoxicity of nondoped Zn(Se,S) QDs and Ni-doped ZnSeS QDs at concentrations of 50µg/mL was evaluated on PANC-1 at different intervals of UV-exposure (Figure 9). The PANC-1 viability was not affected by the presence of UV-irradiated Ni-doped Zn(Se,S) QDs after 60 minutes of UV irradiation. The viability of the PANC-1 exposed to Ni-doped Zn(Se,S) QDs was 92 ± 3% after a period of 60 minutes of UV irradiation. The cell viability for the control test (only cells irradiated with UV during 60 minutes) was 90 ± 3%. In this way, the cell viability for nondoped Zn(Se,S) QDS decreases as much as 36 ± 4% after 60 min under UV-irradiation. The observed increase in cytotoxicity for nondoped Zn(Se,S) QDs could be explained by the production of toxic species like singlet oxygen and ROS induced by the UV excitation. UV-irradiated Ni-doped Nanostructures at high concentration (500 μg/mL) were also evaluated, evidenced absence of toxicity to PANC-1.Figure 9
Effect of UV irradiation time on PANC-1 viability. The final concentration of the nondoped and Ni-doped Zn(Se,S) QDs was 50μg/mL. Blanks were cells incubated with no QDs. Data showed the mean value ± SD of three independent experiments (n
=
3).


## 4. Conclusions

Water-stable Ni-doped Zn(Se,S) QDs and nondoped Zn(Se,S) QDs were synthesized under microwave irradiation at 140°C.It was found that Ni-doped Zn(Se,S) QDs are less toxic than nondoped Zn(Se,S) QDs to PANC-1. The viability of PANC-1 exposed to Ni-doped QDs was nearly 100% at 500μg/mL of concentration. However, the viability of PANC-1 exposed to nondoped QDs was decreased when nondoped QDs at concentrations of 200 μg/mL and higher were used. Furthermore, UV-irradiated Ni-doped nanostructures (50 µg/mL) did not evidence toxicity to cancer cells. The latter mentioned results suggest the potential use of Ni-doped Zn(Se,S) QDs in biological applications.



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*Source: 702391-2015-01-29.xml*