# 124Iodine: A Longer-Life Positron Emitter Isotope—New Opportunities in Molecular Imaging

Authors: Giuseppe Lucio Cascini; Artor Niccoli Asabella; Antonio Notaristefano; Antonino Restuccia; Cristina Ferrari; Domenico Rubini; Corinna Altini; Giuseppe Rubini
Journal: BioMed Research International
 (2014)

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## Abstract

124Iodine (124I) with its 4.2 d half-life is particularly attractive for in vivo detection and quantification of longer-term biological and physiological processes; the long half-life of 124I is especially suited for prolonged time in vivo studies of high molecular weight compounds uptake. Numerous small molecules and larger compounds like proteins and antibodies have been successfully labeled with 124I. Advances in radionuclide production allow the effective availability of sufficient quantities of 124I on small biomedical cyclotrons for molecular imaging purposes. Radioiodination chemistry with 124I relies on well-established radioiodine labeling methods, which consists mainly in nucleophilic and electrophilic substitution reactions. The physical characteristics of 124I permit taking advantages of the higher PET image quality. The availability of new molecules that may be targeted with 124I represents one of the more interesting reasons for the attention in nuclear medicine. We aim to discuss all iodine radioisotopes application focusing on 124I, which seems to be the most promising for its half-life, radiation emissions, and stability, allowing several applications in oncological and nononcological fields.


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## Body

## 1. Introduction

The use of radiopharmaceuticals for molecular imaging of biochemical and physiological processesin vivo has evolved into an important diagnostic tool in modern nuclear medicine and medical research. Positron emission tomography (PET) is currently the most advanced molecular imaging methodology, mainly due to its unrivalled high sensitivity, which allowsin vivo studying of molecular biochemistry. The most frequently used radionuclides for PET have relatively short half-lives (e.g., 11C: 20.4 min; 18F: 109.8 min) which may limit both the synthesis procedures of different radiopharmaceuticals and the time frame of longer biometabolic processes ofin vivo studies. Radionuclides of iodine are widely used in nuclear medicine to label monoclonal antibodies, receptors, and other pharmaceuticals in diagnostic and therapeutic applications where quantitative imaging over a period of several days is necessary [1].Unfortunately, the nuclides most commonly used,131Iodine (131I), 123Iodine (123I), and 125Iodine (125I), have limitations [2].Due to its beta emission (606 keV, 90%),131I is often used for therapy. It also emits some gamma photons that can be used for SPECT imaging. Their main emission energy peak is about 364 keV and requires the use of a high energy all purpose (HEAP) collimator. However, its spectrum of emission is complex and some emission peaks, above 364 keV, cause high-energy contamination and degrade dosimetry.123I is more suitable for imaging. The energy of its main gamma emission peak is 159 keV, which is close to the 140 keV from Tc-99m for which gamma-camera design has traditionally been optimized. 123I can be imaged in a SPECT system with a low energy high resolution (LEHR) collimator, optimized for the Tc-99m (140 keV), or with a medium energy (ME) collimator, optimized for energies up to 300 keV. However, this radionuclide also emits peaks of a higher energy, which again can be responsible for high-energy contamination [3]. Another limit of 123I-labeled radiopharmaceuticals is their short physical half-lives, which permits only the rapid compounds synthesis and the study of short metabolic processes.125I has mainly X-ray energy emission at 27 keV, with low gamma emission at 35.5 keV. It has a photon energy too low for optimal imaging, especially quantitative imaging, and its half-life is undesirably long (42 days). For these reasons, it has not found clinical applications to date [4].124Iodine (124I) is an alternative long-lived PET radionuclide attracting increasing interest for long-term clinical and PET studies. 124I, a positron-emitting nuclide with a half-life of 4.2 days, could permit PET quantitative imaging over several days. Only about 23% of disintegrations result in positron emission, and these are of relatively high energy [5]. In fact, 124I has appropriate physical characteristics, in certain PET radiopharmaceuticals, which can be utilized at low dose; also the spatial resolution of images for this radionuclide is comparable to that obtained with the more conventional PET ones, and the half-life of 4.2 days is appropriate for slow physiological processes also thanks to the clearance of nonspecific radioactivity [6].PET systems are using an electronic collimation based on interaction time. The emission spectrum of124I is also very complex. In 50% of the cases, the emission of a positron is followed by the emission of a gamma of 602 keV, which is likely to create a false coincidence (similar to scattered coincidences). However, it offers the best image quality due to its electronic collimation, which gives a better efficiency of detection and resolution. The short coincidence window also limits the influence of high-energy peaks. For these reasons, 124I seems to be the most promising iodine isotope for an individual pretherapeutic dosimetry [7].


## 2. Physical Data of124I

124I has dual energy emission: beta radiation emissions of 1532 keV (11%) and 2135 keV (11%) and gamma emissions of 511 keV (46%), 603 keV (61%), and 1691 keV (11%). The gamma constant is 2.05E-4 mSv/hr per MBq @ 1.0 meter. The physical half-time (T1/2) is of 4.18 days, the biological half-time is of 120–138 days, and the effective half-time is of 4 days. The critical organ is the thyroid gland. The radiotoxicity differs if the 124I is ingested (2.82E-7 Sv/Bq) or inhaled (1.69E-7 Sv/Bq). The intake routes for 124Iodine may be ingestion, inhalation, puncture, wound, and skin contamination [8].According to these physical properties,124I is the only long-life positron emitter isotope of iodine that may be used for both imaging and therapy as well as for 131I dosimetry. The therapeutic effect of 124I relies on the Auger electron emission responsible for the local action within a few nanometers; the full cell killing becomes evident when a 124I molecule decays within the DNA molecule mainly if placed between the strands.However, if the124Iodine physical properties are favorable for clinical practice, two major features of the radioactive decay should to be accounted for. One is the image resolution due to the high energy of emitted positron with long range; the other is a high fraction of nonpositron decays accompanied by single photon emission in the same energy window. These factors negatively affect resolution and the signal to noise ratio, as well as the fact that they may limit the quantitative evaluation of PET images. More recently, new algorithms of reconstruction and more specific set-up for isotopes different from 18F have been implemented to reduce the image degradation.


## 3. Isotope Production

One of the first schemes for124I production has been based on 124Te(d,2n)124I nuclear reaction. More recently, however, with the increase in the number of low-energy proton cyclotrons, the 124Te(p,n)124I reaction has been reaching popularity because it offers the chance of obtaining the highest levels of purity [9]. The two main contaminants affecting iodine preparation are related to the presence of Tellurium isotopes (due to the target physical properties) and to a mixture of 125I and 123I isotopes for competitive reaction [10]. According to their different half-life, the 125I reduces 124I purity during time. Because the development of competing reactions may increase with the irradiation energy, it is common to select an optimal irradiation energy window to maximize the production minimizing impurities. Other alternative schemes for 124I production include the use of enriched Tellurium (125Te (p,2n) 124I and 126Te(p,3n)124I), characterized by higher 124I production with a high level of long-life radioactive contaminants, or reactions with nontellurium-based target typically with antimony [11–13]. Factors influencing the 124I production are the thermal performance of target, the target composition, and the iodine separation. The target temperature is a critical aspect during production because high yields require high currents of irradiation limited by thermal performance of the target material that may be compensated by an efficient cooling system. Therefore, the irradiation is effective if target temperature is appropriate for thermal performance of target material avoiding volatile isotopes production. Moreover, the thermal stability and performance are related to the chemical form of the target. Commonly, targets for iodine production are composed of Tellurium as TeO2 that warrants a good thermal efficiency. More recently, Al2O has been added to TeO2 providing a better target uniformity, and new promising targets with Al2Te3 have been proposed [14]. Finally, 124I is separated with the dry distillation method by using a vapor pressure in a quartz tube that removes radioactive iodine from the target [15].


## 4. Radiochemistry of124I

The iodination procedure with124I has to consider the physical half-life of the radionuclide and the small-scale concentrations and may be performed by using two main types of chemical reactions: electrophilic and nucleophilic substitution (Table 1).Table 1
Principal radiopharmaceutical labeled with124I.
Radiopharmaceutical
Chemical reaction
Function
124I-MIBG
Nucleophilic exchange
Adrenergic activity
124I-IAZA
Nucleophilic exchange
Hypoxia agent
124I-IAZG
Nucleophilic exchange
Hypoxia agent
124I-dRFIB
Nucleophilic exchange
Cell proliferation
124I-IUdR
Direct electrophilic substitution on activated aromatic systems
Cell proliferation
124I-labeled-hypericin
Direct electrophilic substitution on activated aromatic systems
Protein-kinase C
124I-FIAU
Direct electrophilic substitution on activated aromatic systems
Herpes virus thymidine kinase
m-124I-IPPM
Electrophilic radioiododestannylation reactions
Opioid receptors
124I-IPQA
Electrophilic radioiododestannylation reactions
EGFR kinase activity
124I-labeled-6-anilino-quinazoline deriv.
Electrophilic radioiododestannylation reactions
EGFR inhibitors
124I-label.purpurinimide derivatives
Electrophilic radioiododestannylation reactions
Tumor imaging
124I-label.CDK4/6 inhibitors
Electrophilic radioiododestannylation reactions
Cell proliferationThe direct electrophilic substitution approaches were initially developed for protein labeling and later broadened to aromatic compounds. As observed in thyroid hormones synthesis, the tyrosine present in peptides or proteins is the preferred site for radiolabeling. In this chemical reaction, iodide in oxidation state −1 is oxidized by common reagents such as chloramine-T or Iodogen to form a reactive, electrophilic species in the oxidation state +1, which simply substitutes an activated proton from the aromatic ring of tyrosine in the ortho position to the phenol group. The method is simple and usually high yields are obtained. The main disadvantage is the low selectivity if more than one tyrosine moiety is present with also low stabilityin vivo. Moreover, electrophilic radioiodinations can be also performed by means of various demetallation techniques. Demetallation reactions require organometallic compounds as precursors. An important difference from the direct electrophilic substitution is that nonactivated aromatic compounds can be labeled with good or excellent yields. In addition, due to the lack of activating groups, the labeled compounds usually show a much higher metabolic stability when compared to the activated aromatic compounds.The nucleophilic substitution represents the more simple method for direct iodination using iodide as nucleophile in the oxidation state −1. This exchange reaction can occur in aliphatic and aromatic compounds but proceeds slowly on aromatic ones. An example of this is the exchange of stable iodine, bound to the precursor by radioactive iodine (isotope exchange). This can be achieved by simply heating the components in a suitable solvent such as acetone or water. The consequence, however, is a product of low specific activity and therefore used only in very selected cases, for example, with124I-MIBG.Finally, other methods have been developed, including the direct labeling of proteins through radioiodination of tyrosine residues with electropositive radioiodine. Chloramine T and various oxidative enzymes are useful oxidizing agents for the in situ oxidation of radioiodine for direct protein labeling. Another approach for iodination employs the prosthetic groups. Prosthetic groups are bifunctional reagents; one allows for high yield radiohalogenation, and the other allows for conjugation to the biomolecule. This strategy became known as the “Bolton-Hunter method” [16].


## 5. Clinical Applications of124I

The increased use of imaging with PET is accompanied by a demand for versatile radiopharmaceuticals and positron emitters with relatively long half-life, suitable for the PET procedure and able to highlight the tumor cell characteristics (alteration of enzymology, increased rate of glycolysis, protein and lipid synthesis rate, and DNA syntheses).Among the radioactive iodine radionuclides using nucleophilic substitution reaction, there are124I-MIBG, 124I-IAZA, 124I-IAZG, and 124I-dRFIB. M-Iodobenzylguanidine (MIBG) is used in diagnosis (labeled with 123I or 124I) and therapy (labeled with 131I) of neuroblastoma and pheochromocytoma [17, 18]. The mean effective dose from 124I-MIBG to the adult male human, extrapolated from animal data, was estimated to be 0.25 mSv/MBq. The highest mean equivalent dose was in the thyroid, at 2.343 mSv/MBq [19]. Clinical trials regarding measurements of organ and tumor dosimetry, using 124I-MIBG PET/CT in patients with refractory or relapsed neuroblastoma, and the assessment of the accuracy of tumor imaging using 124I-MIBG PET/CT versus 123I-MIBG scan with 3-dimensional imaging by SPECT or SPECT/CT by number, intensity of uptake, and localization of sites of tumor are in progress [20]. Moroz et al. described the use of 124I-labeled MIBG for imaging norepinephrine transporter (NET) function [21].124I-IAZA and 124I-IAZG are hypoxia imaging agents and were used in a comparative study with two other 2-nitroimidazole derivatives (18F-FMISO and 18F-FAZA) for the visualization of tumor hypoxia in A431 bearing mice by means of PET [22].While124I-dRFIB was synthetized by Stahlschmidt et al. [23] to image cell proliferation, no data on the radiopharmacological evaluation of [124I]dRFIB are reported in practice to date.All the other radioactive iodine radionuclides use electrophilic substitution reaction. One of the first molecules introduced for radiotherapy and labeled with124I was the iododeoxyuridine (IUdR). Radiosynthesis of 5-[124I]iodo-2′-deoxyuridine ([124I]IUdR) for functional imaging of cell proliferation by means of PET was investigated by Guenther et al. A rapid radioiodinationin vivo, resulting in high accumulation of activity in the thyroid, was demonstrated. Twenty-four hours after [124I]IUdR injection, brain tumor imaging was feasible [24].Sang et al. described the synthesis of123I- and 124I-labeled hypericin derivatives. Hypericin, a natural polycyclic aromatic anthraquinone, was used in the treatment of depression and showed antiretroviral activity against several viruses including human immunodeficiency virus (HIV). Additionally, an elevated activity against protein kinase C (PK-C) was found in malignant gliomas. Sang et al. investigated the possibility of using iodine labeled hypericin derivatives for imaging malignant gliomas with PET and SPECT [25].Also,124I would be of important value for detecting the expression of successful gene transduction in target tissue or specific organs. Radioiodinated 2′-fluoro-2′-deoxy-1-β- D-arabinofuranosyl-5-iodouracil (FIAU) has been used to obtain quantitativein vivo PET images of herpes virus thymidine kinase (HSVl-tk) gene expression with superior sensitivity and resolution over that of SPECT 131I-radiolabeled FIAU images and also to monitor clinical gene therapy [26, 27].Studies on central cannabinoid CB1 receptors in schizophrenic patients led to the development of124I-labeled imaging probe n-(morpholin-4-yl)-1-(2,4-dichlorophenyl)-5-(4-[124I]iodophenyl)-4-methyl-1hpyrazole-3-carboxamide ([124I]AM281). Two 124I-labeled cyclin-dependent kinase 4/6 inhibitors were developed to study the role of Cdk 4/6 during cell proliferation in tumor cells. 80% of human tumors show a deregulation of the cell cycle relevant Cdk4-cyclin D1/retinoblastoma (pRb)/E2F signal cascade resulting in uncontrolled tumor growth. Radiolabeled Cdk4 inhibitors have been suggested as promising molecular probes for imaging tumor cell proliferation [28].124I is also important in the study of the expression of multidrug resistance. Colchicine, a potent inhibitor of cellular mitosis, is a member of the multidrug resistance family of drugs. As a potential indicator of resistance, the C-10 methoxy group of n-colchicine has been labeled using 11C- and 13C-iodomethane [29]. However, the restrictions imposed by the short half-life of the carbon-11 compound prompted the investigation into the syntheses of colchicine analogues labeled with radiohalogens.Iozzo et al. [30] prepared 124I-labeled human insulin by direct electrophilic iodination at the A14-tyrosine residue. It retains receptor binding properties and biological activity as the native hormone.The long physical half-life of124I is particularly well suited for labeling large molecules like antibodies. The relatively long half-life allows PET imaging at late time points (>
24 h) ensuring sufficient accumulation of the radiolabeled antibody in the target tissue (e.g., tumor). Various 124I-labeled antibodies have been used for molecular imaging and therapy of differentiated thyroid cancer, breast cancer, colorectal cancer, clear-cell renal cell carcinoma, ovarian cancer, and neuroblastoma.


### 5.1. The124I Experience in Thyroid Cancer

124I appears as one of the more interesting imaging probes between new agents introduced for PET/CT. It emerged in clinical scenario of thyroid cancer patients because of the high spatial resolution of PET images and better sensitivity than 131I [31]. In this setting, 124I combines the well-known diagnostic efficacy of iodide family together with the individual dosimetry before 131I treatment avoiding cellular stunning.124I in PET is being used mainly in the staging of recurrent or residual thyroid malignancy and for pretherapy individualized dosimetry. Moreover a combined use of 124I and 18F-FDG PET/CT improves restaging in recurrent differentiated thyroid cancer (DTC). This combination of PET agents is claimed to better predict the outcome of high-dose 131I therapy and can be used clinically to decide further management [32]. Moreover in the presence of biochemical recurrence with an increased thyroglobulin (Tg), a negative 124I PET can avoid high-dose 131I therapy, which implies the need for further additional imaging to estimate iodine nonavid metastatic disease. Phan et al. [33] showed in their study that 124I PET detected more abnormalities in comparison to the diagnostic whole body 131I scan but showed comparable findings with the posttreatment scan. They reported that only 3 of 11 patients with positive 124I PET scanning, had visible abnormalities in pretherapy scans. Furthermore, PET also proved incremental value by showing lesions in 2 of the 5 patients with undetectable Tg, whereas the whole body iodine scans were negative in all 5 patients. In a comparison between 124I and 18F-FDG PET in 21 DTC patients at staging or with arising Tg level or with Tg antibodies, without cervical lymph nodes at standard imaging, the reported sensitivities were 80% and 70% for 124I and 18F-FDG, respectively [32]. The authors reported an incremental diagnostic value for 124I when coregistered with diagnostic CT scan. However, near 30% of the lesions were concordantly positive on both PET scans, while positive with only one of these modalities in the others. Authors concluded that the combination of 124I and 18F-FDG PET/CT improves restaging in recurrent DTC. Although diagnostic role for 124I PET should be proven in a large comparable series of patients affected by DTC, the lesion-based dosimetry appears as well defined issue that may be addressed with wide consensus. In fact, by using the lesion-based dosimetry, tumor stunning may be avoided, and replacing the fixed iodine dose, the therapeutic effects are now maximized reducing collateral ones. Maxon using 124I has shown that when the radiation dose was greater than 80 Gy, 98% of metastases responded to treatment; on the contrary, only 20% of metastases responded to treatment when the prescribed dose was lower [34]. Furthermore, none of the lesions receiving less than 35 Gy responded to treatment. In a study reported by Dorn et al. in DTC patients over 15 years, they reported in 187 pretherapy 124I PET dosimetric evaluations that the delivered 131I dose was safe for critical organs (red marrow or lungs) and complete response in metastases was achieved with absorbed doses of >
100 Gy [35].


### 5.2.124I-Beta-Cit

Radiolabeled beta-CIT is often used for brain imaging labeled with123I, but FP-CIT is more suitable for imaging of dopamine transporter because of its higher selectivity and faster kinetics. In particular, beta-CIT is affected in clinical practice by higher affinity for serotoninergic receptors than 123I-FP-CIT producing functional images of both neurotransmissions. Moreover, the dopamine binding potential with beta-CIT is obtained after 18 hours from injection, far from an optimal acquisition time for 123I-agents. However, beta-CIT has been demonstrated to visualize the dopamine reuptake in different cortical areas, as well as in mesolimbic or mesocortical regions that are not detected by FP-CIT. For these kinetic differences, beta-CIT is considered a very interesting dopamine tracer, affected in practice by physical properties of 123I. More recently, we have tested in patients affected by Parkinson's disease (PD) a novel radiopharmaceutical, in which beta-CIT is labeled with 124I [36]. It has been obtained by the addition in the following order: 5 mCi of Na124I in a solution of 500 μL of NaOH 0.05 N; 50 μg of trialkylstanyl precursor ([2b-carbomethoxy-3b-(4-tributylstannylphenyl)tropane] dissolved, sonicating for 3 minutes, in 150 μL of ethanol; 50 μL of H3PO4 0.5 N; 50 μL of CH3CO3H 0.02 M prepared at the moment of use by 100 μL 32% of peracetic acid dissolved in 2.4 mL of water. After 30 minutes at ambient temperature in an inert atmosphere, we add 100 μL NaHSO3 in a solution prepared by dissolving 10 mg in 1 mL. In our unpublished study, we have administered 37 MBq of 124I-beta-CIT intravenously in patients affected by PD and essential tremors; then PET images were obtained at 4, 24, and 48 hours from injection. In all patients, we have reported a precise and reproducible evaluation of striatum morphology also in patients with severe PD (Figure 1), as well as of mesolimbic and mesocortical structures probably due to serotoninergic uptake.Figure 1
124I-beta-CIT in severe PD. Note a bilateral symmetric involvement of putamen and extrastriatal uptake at 48 h images.


## 5.1. The124I Experience in Thyroid Cancer

124I appears as one of the more interesting imaging probes between new agents introduced for PET/CT. It emerged in clinical scenario of thyroid cancer patients because of the high spatial resolution of PET images and better sensitivity than 131I [31]. In this setting, 124I combines the well-known diagnostic efficacy of iodide family together with the individual dosimetry before 131I treatment avoiding cellular stunning.124I in PET is being used mainly in the staging of recurrent or residual thyroid malignancy and for pretherapy individualized dosimetry. Moreover a combined use of 124I and 18F-FDG PET/CT improves restaging in recurrent differentiated thyroid cancer (DTC). This combination of PET agents is claimed to better predict the outcome of high-dose 131I therapy and can be used clinically to decide further management [32]. Moreover in the presence of biochemical recurrence with an increased thyroglobulin (Tg), a negative 124I PET can avoid high-dose 131I therapy, which implies the need for further additional imaging to estimate iodine nonavid metastatic disease. Phan et al. [33] showed in their study that 124I PET detected more abnormalities in comparison to the diagnostic whole body 131I scan but showed comparable findings with the posttreatment scan. They reported that only 3 of 11 patients with positive 124I PET scanning, had visible abnormalities in pretherapy scans. Furthermore, PET also proved incremental value by showing lesions in 2 of the 5 patients with undetectable Tg, whereas the whole body iodine scans were negative in all 5 patients. In a comparison between 124I and 18F-FDG PET in 21 DTC patients at staging or with arising Tg level or with Tg antibodies, without cervical lymph nodes at standard imaging, the reported sensitivities were 80% and 70% for 124I and 18F-FDG, respectively [32]. The authors reported an incremental diagnostic value for 124I when coregistered with diagnostic CT scan. However, near 30% of the lesions were concordantly positive on both PET scans, while positive with only one of these modalities in the others. Authors concluded that the combination of 124I and 18F-FDG PET/CT improves restaging in recurrent DTC. Although diagnostic role for 124I PET should be proven in a large comparable series of patients affected by DTC, the lesion-based dosimetry appears as well defined issue that may be addressed with wide consensus. In fact, by using the lesion-based dosimetry, tumor stunning may be avoided, and replacing the fixed iodine dose, the therapeutic effects are now maximized reducing collateral ones. Maxon using 124I has shown that when the radiation dose was greater than 80 Gy, 98% of metastases responded to treatment; on the contrary, only 20% of metastases responded to treatment when the prescribed dose was lower [34]. Furthermore, none of the lesions receiving less than 35 Gy responded to treatment. In a study reported by Dorn et al. in DTC patients over 15 years, they reported in 187 pretherapy 124I PET dosimetric evaluations that the delivered 131I dose was safe for critical organs (red marrow or lungs) and complete response in metastases was achieved with absorbed doses of >
100 Gy [35].


## 5.2.124I-Beta-Cit

Radiolabeled beta-CIT is often used for brain imaging labeled with123I, but FP-CIT is more suitable for imaging of dopamine transporter because of its higher selectivity and faster kinetics. In particular, beta-CIT is affected in clinical practice by higher affinity for serotoninergic receptors than 123I-FP-CIT producing functional images of both neurotransmissions. Moreover, the dopamine binding potential with beta-CIT is obtained after 18 hours from injection, far from an optimal acquisition time for 123I-agents. However, beta-CIT has been demonstrated to visualize the dopamine reuptake in different cortical areas, as well as in mesolimbic or mesocortical regions that are not detected by FP-CIT. For these kinetic differences, beta-CIT is considered a very interesting dopamine tracer, affected in practice by physical properties of 123I. More recently, we have tested in patients affected by Parkinson's disease (PD) a novel radiopharmaceutical, in which beta-CIT is labeled with 124I [36]. It has been obtained by the addition in the following order: 5 mCi of Na124I in a solution of 500 μL of NaOH 0.05 N; 50 μg of trialkylstanyl precursor ([2b-carbomethoxy-3b-(4-tributylstannylphenyl)tropane] dissolved, sonicating for 3 minutes, in 150 μL of ethanol; 50 μL of H3PO4 0.5 N; 50 μL of CH3CO3H 0.02 M prepared at the moment of use by 100 μL 32% of peracetic acid dissolved in 2.4 mL of water. After 30 minutes at ambient temperature in an inert atmosphere, we add 100 μL NaHSO3 in a solution prepared by dissolving 10 mg in 1 mL. In our unpublished study, we have administered 37 MBq of 124I-beta-CIT intravenously in patients affected by PD and essential tremors; then PET images were obtained at 4, 24, and 48 hours from injection. In all patients, we have reported a precise and reproducible evaluation of striatum morphology also in patients with severe PD (Figure 1), as well as of mesolimbic and mesocortical structures probably due to serotoninergic uptake.Figure 1
124I-beta-CIT in severe PD. Note a bilateral symmetric involvement of putamen and extrastriatal uptake at 48 h images.


## 6. Conclusions

The iodine isotopes with particular regard to131I and 123I have represented the cornerstone of nuclear medicine, in thyroid diseases especially. Nowadays, this family has been enriched by 124I, the longer positron emitter of iodine, which for the physical properties has gained a role in the clinical practice of molecular imaging.In fact,124I joins a suitable radioactive emission to a favorable, simple, and well-documented radiochemistry as well as standardized production and target processing. All these aspects together with the high quality of PET technology warrant an actual role in thyroid cancer imaging, as well as promising applications in neurology and oncology.The future advances in probe development will lead to the production of novel innovative radiopharmaceuticals for specific molecular targeting for both imaging and therapy where the emission of Auger electrons is associated with high resolution quantitative images.



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