# Evaluation of Analgesic and Anti-Inflammatory Activities of Water Extract ofGalla Chinensis In Vivo Models

Authors: Kai Sun; Xu Song; RenYong Jia; Zhongqiong Yin; Yuanfeng Zou; Lixia Li; Lizi Yin; Changliang He; Xiaoxia Liang; Guizhou Yue; Qiankun Cui; Yi Yang
Journal: Evidence-Based Complementary and Alternative Medicine
 (2018)

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## Abstract

Aim. Pain and inflammation are associated with many diseases in humans and animals.Galla Chinensis, a traditional Chinese medicine, has a variety of pharmacological properties. The purpose of this study was to evaluate analgesic and anti-inflammatory activities ofGalla Chinensisthrough different animal models.Method. The analgesic activities were evaluated by hot-plate and writhing tests. The anti-inflammatory effects were assessed by ear edema, capillary permeability, and paw edema tests. The contents of cytokines (NO, iNOS, PGE2, and IL-10) in serum of rats in paw edema test were inspected by ELISA assays.Results. In the hot-plate test,Galla Chinensis could significantly extend pain threshold when compared to control group. The inhibitory rates of writhes ranged from 36.62% to 68.57% inGalla Chinensis-treated mice. Treatment withGalla Chinensis (1 and 0.5 g/kg) could significantly inhibit ear edema (47.45 and 36.91%, resp.; P < 0.01).Galla Chinensis (1 g/kg) had significant (P < 0.05) anti-inflammatory activity in capillary permeability test (29.04%). In carrageenan-induced edema test, the inhibitory rates were 43.71% and 44.07% (P < 0.01) at 1 h and 2 h after administration ofGalla Chinensis(1 g/kg), respectively, and the levels of proinflammatory cytokines were significantly reduced.Conclusion. These results suggest thatGalla Chinensis has analgesic and anti-inflammatory effects, which may be a candidate drug for the treatment of inflammation and pain.


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## Body

## 1. Introduction

Recent studies revealed that inflammation was associated not only with ailments, wounds, trauma, and swelling [1], but also with cancer, atherosclerosis, cardiovascular disease, arthritis, neurodegenerative disease, diabetes mellitus, and obesity [2]. Some physical, chemical, and biological stimuli can lead to occurrence of inflammation. It is usually accompanied with pain, redness, swelling, heat, and dysfunction [3]. The primary treatment of inflammation and pain is to use nonsteroidal anti-inflammatory drugs, but long-term use could lead to a lot of side effects, such as cardiovascular and gastrointestinal complications [4, 5]. Therefore, it is necessary to develop new drugs for treatment of inflammation and pain.Galla Chinensis, a traditional Chinese herbal medicine, is widely distributed in Guizhou, Sichuan, Hubei, Hunan, Shanxi, and Yunnan. It originates from the plants of familyAnacardiaceae (mainlyRhus chinensisMill,Rhus potaniniiMaxim, andRhus punjabensis) parasitized by the Chinese sumac aphid Baker [6, 7]. The main ingredient is tannin acid.In vitro andin vivo studies have demonstrated its pharmacological effects, including spermicidal, antibacterial, and antioxidant activities [8, 9]. However, there are no reports about the analgesic and anti-inflammatory activities. Therefore, in this study, the analgesic effects ofGalla Chinensis were determined through hot-plate test and acetic acid-induced writhing, and the anti-inflammatory activities were assessed through xylene-induced ear edema test, acetic acid-induced capillary permeability, and carrageenin-induced paw edema test for developing a new drug to treat inflammation and pain.


## 2. Materials and Methods

### 2.1. Animals

Male Sprague-Dawley rats (200 ± 20 g) and male or female Kunming mice (20 ± 2 g) were purchased from Chengdu Dossy Experimental Animals Co. Ltd. (License number SCXK (Sichuan) 201509). The experimental protocol was approved by the National Institute of Ethics Committee at Sichuan Agricultural University [approval number SYXK (Sichuan) 2014-187]. All animals were maintained in animal room with free access to water and standard diet and 12 h light-dark cycle. They were acclimatized at least 1 week before experiments started.


### 2.2. Drug and Chemicals

Galla Chinensis (number 160512) was bought from Baoji F. S. Biological Development Co. Ltd. (Shanxi, China) and identified by Dr. Lixia Li (Sichuan Agricultural University, China). Nitrogen monoxide (NO), nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and interleukin-10 (IL-10) were all bought from Nanjing Jiancheng Biotechnology Co., Ltd. (Nanjing, China).


#### 2.2.1. Extraction

Galla Chinensis powder (100 g) was decocted in water (1500 mL) for 2 h, and then the decoction was concentrated under reduced pressure. Finally, theGalla Chinensis extract (gallotannins) was obtained by lyophilization [10–12]. Referring to the method in the Chinese Pharmacopoeia, tannin of crude extract was determined by a tungsten molybdophosphate-casein colorimetric method. The results show that the content of tannin in crude extract is 92.5% [13].Galla Chinensis extract (gallotannins) was dissolved in distilled water and administered orally. Indomethacin (positive control) was purchased from Shanxi Taiyuan Pharmaceutical Co. Ltd. (Shanxi, China) and dissolved in 0.5% (w/v) sodium carboxymethyl cellulose.


### 2.3. Hot-Plate Test

The hot-plate test was conducted as the methods previously reported [14, 15]. The temperature of electrical hot-plate was set at 55 ± 0.5°C. The female mice were put on electrical hot-plate, and the time was recorded when the mice showed any nociceptive indicators. The pain indicators consisted of lifting, licking paw, and jumping. A pain threshold of the female mice (the time when they first exhibit one of the pain indicators) within 5~30 s was used for the test. The mice were treated once a day with physiological saline,Galla Chinensis(1, 0.5, and 0.25 g/kg) and indomethacin (2 mg/kg) for 4 days, respectively. The pain threshold was measured at 30, 60, 90, and 120 min after the last administration, respectively. If the times were more than 60 s, the mice were taken out immediately and the response time was recorded as 60 s.


### 2.4. Acetic Acid-Induced Writhing in Mice

The acetic acid-induced writhing test was performed as previously described [16]. Mice were divided into five groups, and each group contained five male and five female mice. The mice received physiological saline, indomethacin (2 mg/kg), andGalla Chinensis at doses of 1, 0.5, and 0.25 g/kg for four consecutive days, respectively. The mice were intraperitoneally infected with 0.7% (v/v) acetic acid (0.01 mL/g) at 1 h after the last administration. The mice were then placed in an observation box, and the total number of writhes was recorded for 20 min after injection. The percentage of analgesia was calculated by the following equation:(1)%Inhibition=Nm-NcNm×100,where Nm is the number of writhes in control group; Nc is the number of writhes in treated groups.


### 2.5. Xylene-Induced Ear Edema

Mice were divided into five groups, and each group contained five male and five female mice. In control group, the mice received physiological saline (0.1 mL/10 g); indomethacin (2 mg/kg) was served as positive control;Galla Chinensis was administrated at doses of 1 g/kg, 0.5 g/kg, and 0.25 g/kg, respectively. The doses were administered orally to the mice each day for 4 consecutive days, respectively. At 1 h after the last administration, xylene (50 uL) was smeared on the surface of right ear [17]. The left ear was used as control. All animals were sacrificed at 1 h after smear, and then two ears along the auricle were cut off. The auricle with diameter of 8 mm was taken and weighed. The inhibition rate was calculated according to the following formula:(2)%Edema  degree=Mright-Mleft,%Inhibition=Edemadegreecontrol-EdemadegreetreatedEdemadegreecontrol×100,where Mright is the weight of right piece and Mleft is the weight of left piece.


### 2.6. Acetic Acid-Indeed Capillary Permeability

Mice were divided into five groups, and each had ten mice (5 female and 5 male). The mice were given physiological saline, indomethacin (positive control; 2 mg/kg), andGalla Chinensis (1, 0.5, and 0.25 g/kg) once a day for 4 days, respectively. Evans blue (0.5%) was injected into tail vein at 1 h after last administration. The mice were intraperitoneally injected with 0.2 mL 0.7% (v/v) acetic acid. After 20 min, the pleural cavity was repeatedly washed with 6 ml saline, and then the liquid was collected and centrifuged at 3000 rpm for 10 min. The OD values at 590 nm of the supernatant were measured by a spectrophotometer [18].


### 2.7. Carrageenan-Induced Rat Paw Edema

The carrageenan-induced paw edema test was conducted according to the method previously reported [19]. Male rats were randomly divided into five groups (n = 8). The mice received physiological saline,Galla Chinensis(1, 0.5, and 0.25 g/kg), and indomethacin (positive control; 2 mg/kg), respectively, for 4 successive days. At 60 min after the last treatment, mice were infected with 0.1 mL of carrageenan suspension (0.1 g/mL) in the right hind paw. The swelling degree of paw was reflected by examining the average volume of paw swelling at 1, 2, 3, 4, 5, and 6 h, respectively. The edema degree was calculated according to the following formula:(3)%Inhibition=V1-V2V2×100,%Inhibition=Edema  degreecontrol-Edema  degreetreatedEdema  degreecontrol×100,where V1 is the average volume of paw swelling at different time per group. V2 is the average volume of paw before injecting carrageenan per group.After the last measurement, all rats were anesthetized with ether, and the blood was gathered in vacuum tubes with no heparin sodium from abdominal aorta. After coagulation, the blood was centrifuged at 3000 rpm for 10 min and serum was collected. The contents of cytokines in serum were inspected by the ELISA assays, including NO, iNOS, PGE2, and IL-10.


### 2.8. Statistical Analysis

All statistical analysis was determined using SPSS 19.0 statistical software and one-way analysis of variance (ANOVA) in Duncan’s test.P < 0.05 was regarded as statistically significant and P < 0.01 was considered very significant. The data were expressed as mean value ± standard deviation (M ± SD).


## 2.1. Animals

Male Sprague-Dawley rats (200 ± 20 g) and male or female Kunming mice (20 ± 2 g) were purchased from Chengdu Dossy Experimental Animals Co. Ltd. (License number SCXK (Sichuan) 201509). The experimental protocol was approved by the National Institute of Ethics Committee at Sichuan Agricultural University [approval number SYXK (Sichuan) 2014-187]. All animals were maintained in animal room with free access to water and standard diet and 12 h light-dark cycle. They were acclimatized at least 1 week before experiments started.


## 2.2. Drug and Chemicals

Galla Chinensis (number 160512) was bought from Baoji F. S. Biological Development Co. Ltd. (Shanxi, China) and identified by Dr. Lixia Li (Sichuan Agricultural University, China). Nitrogen monoxide (NO), nitric oxide synthase (iNOS), prostaglandin E2 (PGE2), and interleukin-10 (IL-10) were all bought from Nanjing Jiancheng Biotechnology Co., Ltd. (Nanjing, China).


### 2.2.1. Extraction

Galla Chinensis powder (100 g) was decocted in water (1500 mL) for 2 h, and then the decoction was concentrated under reduced pressure. Finally, theGalla Chinensis extract (gallotannins) was obtained by lyophilization [10–12]. Referring to the method in the Chinese Pharmacopoeia, tannin of crude extract was determined by a tungsten molybdophosphate-casein colorimetric method. The results show that the content of tannin in crude extract is 92.5% [13].Galla Chinensis extract (gallotannins) was dissolved in distilled water and administered orally. Indomethacin (positive control) was purchased from Shanxi Taiyuan Pharmaceutical Co. Ltd. (Shanxi, China) and dissolved in 0.5% (w/v) sodium carboxymethyl cellulose.


## 2.2.1. Extraction

Galla Chinensis powder (100 g) was decocted in water (1500 mL) for 2 h, and then the decoction was concentrated under reduced pressure. Finally, theGalla Chinensis extract (gallotannins) was obtained by lyophilization [10–12]. Referring to the method in the Chinese Pharmacopoeia, tannin of crude extract was determined by a tungsten molybdophosphate-casein colorimetric method. The results show that the content of tannin in crude extract is 92.5% [13].Galla Chinensis extract (gallotannins) was dissolved in distilled water and administered orally. Indomethacin (positive control) was purchased from Shanxi Taiyuan Pharmaceutical Co. Ltd. (Shanxi, China) and dissolved in 0.5% (w/v) sodium carboxymethyl cellulose.


## 2.3. Hot-Plate Test

The hot-plate test was conducted as the methods previously reported [14, 15]. The temperature of electrical hot-plate was set at 55 ± 0.5°C. The female mice were put on electrical hot-plate, and the time was recorded when the mice showed any nociceptive indicators. The pain indicators consisted of lifting, licking paw, and jumping. A pain threshold of the female mice (the time when they first exhibit one of the pain indicators) within 5~30 s was used for the test. The mice were treated once a day with physiological saline,Galla Chinensis(1, 0.5, and 0.25 g/kg) and indomethacin (2 mg/kg) for 4 days, respectively. The pain threshold was measured at 30, 60, 90, and 120 min after the last administration, respectively. If the times were more than 60 s, the mice were taken out immediately and the response time was recorded as 60 s.


## 2.4. Acetic Acid-Induced Writhing in Mice

The acetic acid-induced writhing test was performed as previously described [16]. Mice were divided into five groups, and each group contained five male and five female mice. The mice received physiological saline, indomethacin (2 mg/kg), andGalla Chinensis at doses of 1, 0.5, and 0.25 g/kg for four consecutive days, respectively. The mice were intraperitoneally infected with 0.7% (v/v) acetic acid (0.01 mL/g) at 1 h after the last administration. The mice were then placed in an observation box, and the total number of writhes was recorded for 20 min after injection. The percentage of analgesia was calculated by the following equation:(1)%Inhibition=Nm-NcNm×100,where Nm is the number of writhes in control group; Nc is the number of writhes in treated groups.


## 2.5. Xylene-Induced Ear Edema

Mice were divided into five groups, and each group contained five male and five female mice. In control group, the mice received physiological saline (0.1 mL/10 g); indomethacin (2 mg/kg) was served as positive control;Galla Chinensis was administrated at doses of 1 g/kg, 0.5 g/kg, and 0.25 g/kg, respectively. The doses were administered orally to the mice each day for 4 consecutive days, respectively. At 1 h after the last administration, xylene (50 uL) was smeared on the surface of right ear [17]. The left ear was used as control. All animals were sacrificed at 1 h after smear, and then two ears along the auricle were cut off. The auricle with diameter of 8 mm was taken and weighed. The inhibition rate was calculated according to the following formula:(2)%Edema  degree=Mright-Mleft,%Inhibition=Edemadegreecontrol-EdemadegreetreatedEdemadegreecontrol×100,where Mright is the weight of right piece and Mleft is the weight of left piece.


## 2.6. Acetic Acid-Indeed Capillary Permeability

Mice were divided into five groups, and each had ten mice (5 female and 5 male). The mice were given physiological saline, indomethacin (positive control; 2 mg/kg), andGalla Chinensis (1, 0.5, and 0.25 g/kg) once a day for 4 days, respectively. Evans blue (0.5%) was injected into tail vein at 1 h after last administration. The mice were intraperitoneally injected with 0.2 mL 0.7% (v/v) acetic acid. After 20 min, the pleural cavity was repeatedly washed with 6 ml saline, and then the liquid was collected and centrifuged at 3000 rpm for 10 min. The OD values at 590 nm of the supernatant were measured by a spectrophotometer [18].


## 2.7. Carrageenan-Induced Rat Paw Edema

The carrageenan-induced paw edema test was conducted according to the method previously reported [19]. Male rats were randomly divided into five groups (n = 8). The mice received physiological saline,Galla Chinensis(1, 0.5, and 0.25 g/kg), and indomethacin (positive control; 2 mg/kg), respectively, for 4 successive days. At 60 min after the last treatment, mice were infected with 0.1 mL of carrageenan suspension (0.1 g/mL) in the right hind paw. The swelling degree of paw was reflected by examining the average volume of paw swelling at 1, 2, 3, 4, 5, and 6 h, respectively. The edema degree was calculated according to the following formula:(3)%Inhibition=V1-V2V2×100,%Inhibition=Edema  degreecontrol-Edema  degreetreatedEdema  degreecontrol×100,where V1 is the average volume of paw swelling at different time per group. V2 is the average volume of paw before injecting carrageenan per group.After the last measurement, all rats were anesthetized with ether, and the blood was gathered in vacuum tubes with no heparin sodium from abdominal aorta. After coagulation, the blood was centrifuged at 3000 rpm for 10 min and serum was collected. The contents of cytokines in serum were inspected by the ELISA assays, including NO, iNOS, PGE2, and IL-10.


## 2.8. Statistical Analysis

All statistical analysis was determined using SPSS 19.0 statistical software and one-way analysis of variance (ANOVA) in Duncan’s test.P < 0.05 was regarded as statistically significant and P < 0.01 was considered very significant. The data were expressed as mean value ± standard deviation (M ± SD).


## 3. Result

### 3.1. Electrical Hot-Plate Study in Mice

As shown in Figure1,Galla Chinensis (1 and 0.5 g/kg) significantly (P < 0.01) extended the threshold when compared to the control group at 60 min and 90 min after administration. Particularly, at 60 min after the administration, the time thresholds ofGalla Chinensis groups were 24.50 ± 4.70 s, 18.05 ± 1.62 s, and 18.55 ± 2.66 s, respectively, which were significantly higher than that in control group (11.24  ±  2.62 s). The pain threshold in positive control group was also significantly (P < 0.05) increased at 30 min and 60 min after the administration.Figure 1
The electrical hot-plate study in mice. The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05; P∗∗ < 0.01.


### 3.2. Acetic Acid-Induced Writhing in Mice

The peripheral analgesic activities ofGalla Chinensis on acetic acid-induced abdominal writhing in mice were shown in Table 1. Compared with the control group, the number of writhes in treated groups (Galla Chinensis and indomethacin) was significantly reduced (P < 0.01). The inhibition rate in the high, medium, and low doses ofGalla Chinensis-treated groups reached 63.38%, 40.85%, and 38.03%, respectively. Indomethacin-treatment (2 mg/kg) also inhibited the writing (52.11%).Table 1
Effects ofGalla Chinensis on the acetic acid-induced writhing in mice.
Group Dose Writhing times Inhibition (%) Control — 35.50 ± 1.91 — Indomethacin 2 mg/kg 17.00 ± 3.83∗∗ 52.11 Galla Chinensis high dose 1 g/kg 13.00 ± 3.37∗∗ 63.38 Galla Chinensis medium dose 0.5 g/kg 21.00 ± 2.94∗∗ 40.85 Galla Chinensis low dose 0.25 g/kg 22.00 ± 4.97∗∗ 38.03 The values are presented as mean ± standard deviation(n=10). The asterisk denotes the significance levels in comparison with the negative control: P∗∗ < 0.01.


### 3.3. Xylene-Induced Ear Edema Test

The effects ofGalla Chinensis on acute inflammation induced by xylene in mice were shown in Table 2. Compared with the control, the inhibition rates in the high (47.45%,P < 0.01) and medium (36.91%,P < 0.05) doses ofGalla Chinensis-treated groups were significantly reduced. Meanwhile, indomethacin-treatment (2 mg/kg) inhibited ear edema with an inhibition rate of 50.12% (P< 0.01).Table 2
Effects of Galla Chinensis on xylene-induced ear edema test.
Group Dose Edema degree (mg) Inhibition/% Control — 13.37 ± 3.37 — Indomethacin 2 mg/kg 6.67 ± 2.50∗∗ 50.12% Galla Chinensis 1 g/kg 7.03 ± 1.23∗∗ 47.45% Galla Chinensis 0.5 g/kg 8.43 ± 2.72∗ 36.91% Galla Chinensis 0.25 g/kg 10.93 ± 2.29 18.21% The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05; P∗∗ < 0.01.


### 3.4. Acetic Acid-Indeed Capillary Permeability

As shown in Figure2, compared with the control group, administration ofGalla Chinensis (1, 0.5, and 0.25 g/kg) dose-dependently inhibited acetic acid-induced capillary permeability, and the high dose ofGalla Chinensis had significant inhibitory effect (P < 0.05). Indomethacin markedly inhibited (P < 0.01) peritoneal capillary permeability induced by acetic acid.Figure 2
Effect of varying doses ofGalla Chinensison acetic acid-indeed capillary permeability. The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05; P∗∗ < 0.01.


### 3.5. Carrageenin-Induced Rat Paw Edema

The results were shown in Table3. Compared with the control, the high dose ofGalla Chinensis (1 g/kg) significantly reduced carrageenin-induced rat paw edema by 20.17% at 1 h and 27.19% at 2 h after administration (P < 0.05). The medium dose ofGalla Chinensis (0.5 g/kg) could significantly inhibit edema by 29.04% (P < 0.05) at 2 h after administration. Meanwhile, the indomethacin (2 mg/kg) significantly decreased carrageenan-induced rat hind paw edema after administration.Table 3
Effects ofGalla Chinensis on carrageenan-induced rat paw edema.
Groups Dose Carrageenan-induced rat paw edema M ± SD (% inhibition of paw volume) 1 h 2 h 3 h 4 h 5 h 6 h Control — 35.83 ± 13.41 48.61 ± 10.29 46.39 ± 13.34 39.59 ± 7.45 39.56 ± 1078 35.82 ± 7.26 Indomethacin 2 mg/kg 22.88 ± 1.88∗ (36.14) 22.55 ± 7.55∗ (53.61) 24.45 ± 11.08∗ (47.29) 26.76 ± 10.37∗ (32.41) 22.88 ± 1.88∗ (42.16) 21.17 ± 10.45∗ (40.90) Galla Chinensis 1 g/kg 20.17 ± 9.48∗ (43.71) 27.19 ± 5.69∗ (44.07) 29.41 ± 3.99(36.60) 31.25 ± 8.20(21.07) 30.19 ± 8.04(23.69) 29.04 ± 6.48(18.93) Galla Chinensis 0.5 g/kg 25.96 ± 13.12(27.55) 29.04 ± 6.48∗ (40.26) 30.13 ± 2.23(35.05) 30.63 ± 7.14(22.63) 31.17 ± 11.72(21.21) 29.94 ± 10.32(16.42) Galla Chinensis 0.25 g/kg 33.38 ± 1.74(6.84) 42.90 ± 13.49(11.75) 39.88 ± 14.66(14.03) 34.61 ± 8.24(12.58) 34.51 ± 5.34(12.77) 35.64 ± 7.61(0.5) The values are presented as mean ± standard deviation(n=8). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05.In order to estimate the analgesic and anti-inflammatory mechanisms ofGalla Chinensis, several chemical mediators involving iNOS, NO, PGE2, and IL-10 were examined by ELISA. As shown in Figure 3, carrageenan-injection induced a remarkable increase in the concentrations of chemical mediators (iNOS, NO, PGE2, and IL-10) in serum. After treatment withGalla Chinensis (1 g/kg), the NO and PGE2 levels in serum were significantly reduced (P < 0.05) when compared with the control group.Galla Chinensis (0.5 g/kg) markedly decreased (P < 0.05) the PGE2 level. Indomethacin could significantly reduce the NO, iNOS, and PGE2 levels. No significant differences in IL-10 were observed between the treated groups and control group.Figure 3
The changes of inflammatory cytokines in each group. The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the control: P∗ < 0.05; P∗∗ < 0.01.
(a) (b) (c) (d)


## 3.1. Electrical Hot-Plate Study in Mice

As shown in Figure1,Galla Chinensis (1 and 0.5 g/kg) significantly (P < 0.01) extended the threshold when compared to the control group at 60 min and 90 min after administration. Particularly, at 60 min after the administration, the time thresholds ofGalla Chinensis groups were 24.50 ± 4.70 s, 18.05 ± 1.62 s, and 18.55 ± 2.66 s, respectively, which were significantly higher than that in control group (11.24  ±  2.62 s). The pain threshold in positive control group was also significantly (P < 0.05) increased at 30 min and 60 min after the administration.Figure 1
The electrical hot-plate study in mice. The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05; P∗∗ < 0.01.


## 3.2. Acetic Acid-Induced Writhing in Mice

The peripheral analgesic activities ofGalla Chinensis on acetic acid-induced abdominal writhing in mice were shown in Table 1. Compared with the control group, the number of writhes in treated groups (Galla Chinensis and indomethacin) was significantly reduced (P < 0.01). The inhibition rate in the high, medium, and low doses ofGalla Chinensis-treated groups reached 63.38%, 40.85%, and 38.03%, respectively. Indomethacin-treatment (2 mg/kg) also inhibited the writing (52.11%).Table 1
Effects ofGalla Chinensis on the acetic acid-induced writhing in mice.
Group Dose Writhing times Inhibition (%) Control — 35.50 ± 1.91 — Indomethacin 2 mg/kg 17.00 ± 3.83∗∗ 52.11 Galla Chinensis high dose 1 g/kg 13.00 ± 3.37∗∗ 63.38 Galla Chinensis medium dose 0.5 g/kg 21.00 ± 2.94∗∗ 40.85 Galla Chinensis low dose 0.25 g/kg 22.00 ± 4.97∗∗ 38.03 The values are presented as mean ± standard deviation(n=10). The asterisk denotes the significance levels in comparison with the negative control: P∗∗ < 0.01.


## 3.3. Xylene-Induced Ear Edema Test

The effects ofGalla Chinensis on acute inflammation induced by xylene in mice were shown in Table 2. Compared with the control, the inhibition rates in the high (47.45%,P < 0.01) and medium (36.91%,P < 0.05) doses ofGalla Chinensis-treated groups were significantly reduced. Meanwhile, indomethacin-treatment (2 mg/kg) inhibited ear edema with an inhibition rate of 50.12% (P< 0.01).Table 2
Effects of Galla Chinensis on xylene-induced ear edema test.
Group Dose Edema degree (mg) Inhibition/% Control — 13.37 ± 3.37 — Indomethacin 2 mg/kg 6.67 ± 2.50∗∗ 50.12% Galla Chinensis 1 g/kg 7.03 ± 1.23∗∗ 47.45% Galla Chinensis 0.5 g/kg 8.43 ± 2.72∗ 36.91% Galla Chinensis 0.25 g/kg 10.93 ± 2.29 18.21% The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05; P∗∗ < 0.01.


## 3.4. Acetic Acid-Indeed Capillary Permeability

As shown in Figure2, compared with the control group, administration ofGalla Chinensis (1, 0.5, and 0.25 g/kg) dose-dependently inhibited acetic acid-induced capillary permeability, and the high dose ofGalla Chinensis had significant inhibitory effect (P < 0.05). Indomethacin markedly inhibited (P < 0.01) peritoneal capillary permeability induced by acetic acid.Figure 2
Effect of varying doses ofGalla Chinensison acetic acid-indeed capillary permeability. The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05; P∗∗ < 0.01.


## 3.5. Carrageenin-Induced Rat Paw Edema

The results were shown in Table3. Compared with the control, the high dose ofGalla Chinensis (1 g/kg) significantly reduced carrageenin-induced rat paw edema by 20.17% at 1 h and 27.19% at 2 h after administration (P < 0.05). The medium dose ofGalla Chinensis (0.5 g/kg) could significantly inhibit edema by 29.04% (P < 0.05) at 2 h after administration. Meanwhile, the indomethacin (2 mg/kg) significantly decreased carrageenan-induced rat hind paw edema after administration.Table 3
Effects ofGalla Chinensis on carrageenan-induced rat paw edema.
Groups Dose Carrageenan-induced rat paw edema M ± SD (% inhibition of paw volume) 1 h 2 h 3 h 4 h 5 h 6 h Control — 35.83 ± 13.41 48.61 ± 10.29 46.39 ± 13.34 39.59 ± 7.45 39.56 ± 1078 35.82 ± 7.26 Indomethacin 2 mg/kg 22.88 ± 1.88∗ (36.14) 22.55 ± 7.55∗ (53.61) 24.45 ± 11.08∗ (47.29) 26.76 ± 10.37∗ (32.41) 22.88 ± 1.88∗ (42.16) 21.17 ± 10.45∗ (40.90) Galla Chinensis 1 g/kg 20.17 ± 9.48∗ (43.71) 27.19 ± 5.69∗ (44.07) 29.41 ± 3.99(36.60) 31.25 ± 8.20(21.07) 30.19 ± 8.04(23.69) 29.04 ± 6.48(18.93) Galla Chinensis 0.5 g/kg 25.96 ± 13.12(27.55) 29.04 ± 6.48∗ (40.26) 30.13 ± 2.23(35.05) 30.63 ± 7.14(22.63) 31.17 ± 11.72(21.21) 29.94 ± 10.32(16.42) Galla Chinensis 0.25 g/kg 33.38 ± 1.74(6.84) 42.90 ± 13.49(11.75) 39.88 ± 14.66(14.03) 34.61 ± 8.24(12.58) 34.51 ± 5.34(12.77) 35.64 ± 7.61(0.5) The values are presented as mean ± standard deviation(n=8). The asterisk denotes the significance levels in comparison with the negative control: P∗ < 0.05.In order to estimate the analgesic and anti-inflammatory mechanisms ofGalla Chinensis, several chemical mediators involving iNOS, NO, PGE2, and IL-10 were examined by ELISA. As shown in Figure 3, carrageenan-injection induced a remarkable increase in the concentrations of chemical mediators (iNOS, NO, PGE2, and IL-10) in serum. After treatment withGalla Chinensis (1 g/kg), the NO and PGE2 levels in serum were significantly reduced (P < 0.05) when compared with the control group.Galla Chinensis (0.5 g/kg) markedly decreased (P < 0.05) the PGE2 level. Indomethacin could significantly reduce the NO, iNOS, and PGE2 levels. No significant differences in IL-10 were observed between the treated groups and control group.Figure 3
The changes of inflammatory cytokines in each group. The values are presented as mean ± standard deviation (n = 10). The asterisk denotes the significance levels in comparison with the control: P∗ < 0.05; P∗∗ < 0.01.
(a) (b) (c) (d)


## 4. Discussion

It is well known that inflammation and pain are the most common diseases in human and animals, and the current treatment is to use steroidal and nonsteroidal anti-inflammatory drugs which have several side effects [20, 21].Galla Chinensis, a well-known traditional Chinese medicine, has a long history of being used for treating a lot of diseases. However, its analgesic and anti-inflammatory features have not been reported. In this study, we showed the potent analgesic and anti-inflammatory activities ofGalla Chinensis in different animal models. The potency is almost equal to the positive drug (indomethacin).The hot-plate test was used to evaluate central analgesic activity, and the peripheral analgesic activity was usually determined through acetic acid-induced writhing test [22]. In this study,Galla Chinensis and indomethacin could obviously increase the pain threshold of mice (Figure 1), indicating thatGalla Chinensis had a potent central analgesic effect. Moreover, the number of writhes induced by acetic acid inGalla Chinensis-treated mice was significantly inhibited (Table 1), implying thatGalla Chinensis had a commendable peripheral analgesic effect. In addition, the analgesic effect ofGalla Chinensis showed a dose-dependent manner in the hot-plate test and acetic acid-induced writhing test.The xylene-induced ear edema, acetic acid-indeed capillary permeability, and carrageenin-induced rat paw edema were the classic models for acute inflammation tests [23, 24]. In xylene-induced ear edema study,Galla Chinensis could significantly inhibit the ear edema and the inhibition rate in the high doseGalla Chinensis-treated group was equal to that in the indomethacin-treated group (Table 2), suggesting thatGalla Chinensis possessed similar anti-inflammatory activity to indomethacin. Acetic acid has been widely used as a noxious agent to induce experimental inflammation [25]. In this study,Galla Chinensis could significantly inhibit peritoneal capillary permeability induced by acetic acid (Figure 2). In the carrageenin-induced rat paw edema study,Galla Chinensis(1 g/kg) significantly inhibited paw edema of rats in the early phase (within 120 min), especially the high dose ofGalla Chinensis. These results indicated thatGalla Chinensis possessed potent inhibitory effects against acute inflammation.Inflammatory mediators are a kind of chemical medium with strongly biological activity, which can not only accommodate the release of it-self, but also activate other media systems that produce a series of cascade amplification reactions for further development of inflammation [26, 27]. It is reported that activated macrophages were irritated by carrageenan, which led to production of inflammatory mediators [28]. As shown in Figure 3, the contents of NO, iNOS, PGE2, and IL-10 were increased in carrageenan-treated groups, suggesting that carrageenan successfully caused inflammation and the inflammatory mediators (NO, iNOS, PGE2, and IL-10) were associated with the inflammatory process. NO is a short-lived small molecule which plays an important role in inflammatory condition. Harmful responses mediated excessive NO production, such as injury, septic shock, and apoptosis [29]. In carrageenan-evoked inflammation study, the contents of NO in the treated (Galla Chinensis and indomethacin) groups were significantly decreased. iNOS can produce excess NO against infections [30]. This study also showed that the levels of iNOS in the treated (Galla Chinensis and indomethacin) groups were decreased. These results suggested that the anti-inflammatory effects ofGalla Chinensis and indomethacin may be attributed to reducing the content of iNOS which led to the reduction of NO.Prostaglandin (especially PGE2) is important for inflammation diagnosis which is the main metabolite of arachidonic acid and is related to many pathophysiological processes, such as inflammation, tissue destruction, and tumor [31, 32]. This study showed that the contents of PGE2 in the treated (Galla Chinensis and indomethacin) groups were all obviously decreased (Figure 3(c)). These results suggested that theGalla Chinensis and indomethacin could slow down the inflammatory symptoms and had a remarkable inhibitory effect on the release of PGE2. Early results showed that IL-10 effectively inhibited the expression of cytokines and had an important role in stimulating immune and inflammatory responses [33]. Therefore, the level of IL-10 is also important for inflammation diagnosis. As shown in Figure 3(d), the contents of IL-10 in the treated (Galla Chinensis and indomethacin) groups were decreased, but no statistical differences were observed. These results suggested thatGalla Chinensis had little influence on the production of IL-10.


## 5. Conclusions

Galla Chinensis has analgesic and anti-inflammatory activities. The possible anti-inflammatory mechanism is attributed to inhibition of the release of inflammatory cytokines and increasing the expression of anti-inflammatory factor. Hence,Galla Chinensis provides a good potential to be used to treat pain and inflammation.



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