# Bioinspired Synthesis ofAcacia senegal Leaf Extract Functionalized Silver Nanoparticles and Its Antimicrobial Evaluation

Authors: Edwina Olohirere Uzunuigbe; Abidemi Paul Kappo; Sixberth Mlowe; Neerish Revaprasadu
Journal: Journal of Nanomaterials
 (2020)

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## Abstract

Synthesizing nanoparticles with the less environmentally malignant approach using plant extract is of great interest; this is because most of the chemical approaches can be very costly, toxic, and time-consuming. Herein, we report the use ofAcacia senegal leaf extracts to synthesize silver nanoparticles (AgNPs) using an environmentally greener approach. Silver ions were reduced using the bioactive components of the plant extracts with observable colour change from faint colourless to a brownish solution as indication of AgNP formation. The structural properties of the as-synthesized AgNPs were characterized using powder X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and UV-Vis absorption spectrum. Antimicrobial assessment of the as-synthesized AgNPs was explored on some strains of gram-positive and gram-negative bacteria. The obtained results indicate that the as-synthesized AgNPs are pure crystallite of cubic phase of AgNPs, fairly dispersed with a size range of 10–19 nm. The AgNPs were found to be small in size and exhibit significant antibacterial activities, suggesting that the as-synthesized AgNPs could be used in the pharmaceutical and food industries as bactericidal agents.


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## Body

## 1. Introduction

The biosynthetic approach in the use of naturally occurring reducing agents such as plant extracts, biomass, and biological molecules has emerged as a simple alternative method to complex chemical method of nanoparticle synthesis. The use of silver particles in the medicinal field can be traced back to more than ten decades ago when silver was first used in medicine before the discovery of antibiotics [1, 2]. Silver nanoparticles are known to have unique properties that make them ideal for various biological and biomedical applications such as in the treatment and prevention of certain diseases, for therapeutic and diagnostic use including biomolecular detection [3–7], and in industry [8]. This is mainly a result of the antimicrobial, antibacterial, antifungal, antiviral, and anti-inflammatory capabilities of the AgNPs [4, 6, 9, 10]. Silver is additionally known to possess high thermal and electrical conductivity thus resulting in its good optical reflectivity as well as various biological and catalytic abilities [7, 11, 12], which has, in turn, resulted in its high demand [13, 14].Although the processes involved in nanoparticle synthesis result in particles possessing different anticipated characteristics, the chemical and physical methods which include UV irradiation, lithography, ultrasonic fields, and photochemical reduction processes for the production of nanoparticles have their own pitfalls in that they are costly, labour-intensive, and toxic to both organisms and the environment [5, 15–18]. Hence, “green” or biogenic synthesis of nanoparticles is now preferred over physicochemical methods because it not only results in more eco-friendly, cost-effective, contamination-free, and nontoxic sustainable nanoparticles but also allows for higher yield of products with better defined characteristics [12, 19–23]. It has been well documented that living plants and bioactive compounds from their extracts such as polyphenols, phenolic acids or proteins, sugars, terpenoids, and alkaloids have the ability to reduce metal ions by acting as electron shuttles and can therefore be used in the bioreduction of harmful metal ions in the synthesis of nanoparticles [17, 20, 22, 24, 25]. Numerous plant parts have been shown to be effective in the reduction of Au and Ag ions for the formation of gold and silver nanoparticles; these include and not limited to lemon grass leaf extracts (Cymbopogon flexuosus), neem (Azadirachta indica), and tamarind (Tamarindus indica) and fruit extract of amla (Emblica officinalis), as well as the biomass of wheat (Triticum aestivum) and oats (Avena sativa) [26]. Through green synthesis, nanoparticles are synthesized within a short time frame in a single-step bottom-up approach where the use of toxic chemicals, high pressure, energy, or temperatures is taken down to a minimum and plant extracts are used as bioreducing agents and precursors [27, 28]. Plants additionally make the “green” process more favourable and exploitable due to their wide distribution, availability, and cost-effectiveness [14, 29]. Moreover, the bioactive compounds in the plant may act as both a reducing and capping agent in nanoparticle synthesis. Synthesis of nanoparticles using extracts from plants can be determined by the phytochemical components of the plant made up of different functional groups [30]. The nature of these phytochemicals can also affect the morphology, size, and shape of nanoparticle synthesized [31].In this study, silver nanoparticles were synthesized from aqueous leaf extracts ofAcacia senegal (Gum Arabic), a plant that grows in a number of sub-Saharan African countries like Sudan, Nigeria, Mauritania, Senegal, Mali, Burkina Faso, Niger, Chad, Cameroon, Somalia, Ethiopia, and Kenya [32–34]. This plant is mainly known for the rich gummy exudate produced by its stem and branches, which is a nonviscous liquid rich in soluble fibres [32, 35, 36]. The gum is used in the food and drink industries as a stabilizer, an emulsifier in the production of soft drinks and beer, and gummy candies, as well as in cosmetic and pharmaceutical industries. The bioactive components of the Acacia senegal leaf extracts have been previously analyzed and found to contain compounds like phenols, flavonoid, alkaloid, saponins, tannins, and terpenoids [37]. These compounds are known to act as reducing agents in the synthesis of metal nanoparticles [38, 39]. Therefore, we used the leaf extracts of the Acacia senegal plant both as reducing and capping agent during the synthesis of silver nanoparticles (AgNPs) in distilled water and the resulting nanoparticles were tested for their antimicrobial activity on selected gram-negative and gram-positive bacteria.


## 2. Experimental

### 2.1. Materials

Silver nitrate (99.2%) and methanol were purchased from Sigma-Aldrich, USA, and used as received.Acacia senegal leaves were collected from the plantation of the Rubber Research Institute of Nigeria (RRIN), Benin City, Nigeria, with assigned voucher number UBHA379 deposited the plant voucher specimen at RRIN Herbarium.


### 2.2. Preparation of Leaf Extract

Fresh and healthy leaves ofAcacia senegal were washed thoroughly in water and air-dried for 10 days and then grounded into a fine powder with a kitchen blender. About 10 g of the fine powder was boiled in 100 mL distilled water for 10 min and subsequently filtered after cooling. Analysis of the phytochemical constituents was carried out using the method described by [40, 41] using plant water extract to ascertain the presence of bioactive compounds. The phytochemicals shown in Table 1 were present in the aqueous extract of Acacia senegal.Table 1
Phytochemical screening ofAcacia senegal aqueous extract.
Phytochemicals
Present (+); absent (−)
Alkaloids
+
Anthraquinones
−
Carbohydrates
+
Cardiac glycosides
+
Flavonoids
+
Phenols
+
Saponins
+
Steroids
+
Tannins
+
Terpenoids
+


### 2.3. Synthesis of Silver Nanoparticles

10 mL of the obtained leaf extract was added to 90 mL of 1.0 mM AgNO3, and the mixture was autoclaved for 10 minutes at 121°C 15 psi; colour change of the mixture was observed from an initial faint yellow to a brownish-yellow colloidal mixture synthesis. The mixture was washed with methanol twice and finally washed with distilled water several times in a centrifuge at 4400 rpm for 5 min in each cycle. The as-synthesized AgNPs were dried in ambient temperature.


### 2.4. Characterization of Silver Nanoparticles

UV-Vis absorption spectra were recorded on a Varian Cary 50 UV/Vis spectrophotometer. Photoluminescence of the particles was analyzed using a PerkinElmer LS55 Luminescence spectrophotometer. FTIR analysis of the samples was carried out using a PerkinElmer FTIR spectrometer in the range of 4000-450 cm-1. TEM analyses were performed using a JEOL 1400 TEM. Samples were prepared by placing a drop of a dilute solution of nanoparticles on Formvar-coated grids (150-mesh) and allowed to dry completely at room temperature and viewed at an accelerating voltage of 100 kV. X-ray diffraction (XRD) studies were done with a Bruker AXS D8-Advance diffractometer equipped with nickel-filtered Cu Kα radiation (λ=1.5406Å) and the 2θ ranged from 20-80° at 40 kV, 40 mA, and room temperature. A Zeiss Ultra Plus FEG SEM equipped with an Oxford detector EDX at 20 kV which uses Aztec software was used for elemental analysis. The samples were carbon-coated using Quorum coater (Model Q150TE).


### 2.5. Antibacterial Studies of Silver Nanoparticles

#### 2.5.1. Zone of Inhibition

The antimicrobial activity of the AgNPs was evaluated using the disc agar diffusion method [42]. Different bacterial strains were grown at 37°C for 24 hours in a 20 mL nutrient broth; microbial cultures were then diluted to 0.5 McFarland standard. Thereafter, standard petri dishes containing Mueller-Hinton agar were inoculated with bacterial culture. Sterile paper disc (6 mm) was impregnated with 10 μL (10 mg/mL in 5% DMSO) of the test extract and placed on the inoculated plates, which were then incubated at 37°C for 24 hours. The next morning, inhibition zones formed around the disc were measured with a transparent ruler in millimetres. This experiment was carried out in triplicates.


#### 2.5.2. Minimum Inhibitory Concentration (MIC) Assay

The microplate broth dilution assay was used to assess the minimal inhibitory concentration of theA. senegal plant extract with slight modifications [43]. A 12-hour-old culture was diluted 1 : 100 with freshly prepared Muller-Hinton broth. Thereafter, about 100 μL of extract (10 mg/mL in 5% DMSO) was added to a multiwell plate containing 100 μL of freshly prepared broth and serially diluted. The plates were then incubated overnight at 37°C. Approximately 20 μL of 2 mg/mL freshly prepared iodonitrotetrazolium chloride was added to each well and incubated for 1 hour at the same temperature. The MIC was defined as the lowest concentration of the extract to inhibit bacterial growth.


#### 2.5.3. Minimum Bactericidal Concentration (MBC) Assay

A loop full of the microorganism in the wells showing little or no growth in the MIC assay was selected and subcultured on petri plates containing an agar for different microbes. The MBC was defined as the lowest concentration that showed no bacterial growth in the subcultures [44].


#### 2.5.4. Lactate Dehydrogenase (LDH) Release Assay

Bacteria-induced cell damage was quantified using the method described by [45]. Wells containing cultured cells were inoculated with 40 μL of Minimum Essential Media (MEM) or tear fluid containing 106 CFU of cytotoxic bacteria/mL. After 3 hours of incubation at 37°C, the supernatant from each well was collected and diluted 1 : 20 with fresh MEM. The quantity of LDH present in the samples was detected by using a cytotoxicity detection kit (Sigma-Aldrich) according to the manufacturer’s instructions and expressed as absorbance at 490 nm. An additional two sets of wells were treated with MEM but without bacteria. One set of cells was used to determine background LDH release, while cells in the other group were lysed with MEM containing Triton X-100 (0.25% v/v) at the end of the assay to determine the amount of LDH released when 100% of the cells were killed.


## 2.1. Materials

Silver nitrate (99.2%) and methanol were purchased from Sigma-Aldrich, USA, and used as received.Acacia senegal leaves were collected from the plantation of the Rubber Research Institute of Nigeria (RRIN), Benin City, Nigeria, with assigned voucher number UBHA379 deposited the plant voucher specimen at RRIN Herbarium.


## 2.2. Preparation of Leaf Extract

Fresh and healthy leaves ofAcacia senegal were washed thoroughly in water and air-dried for 10 days and then grounded into a fine powder with a kitchen blender. About 10 g of the fine powder was boiled in 100 mL distilled water for 10 min and subsequently filtered after cooling. Analysis of the phytochemical constituents was carried out using the method described by [40, 41] using plant water extract to ascertain the presence of bioactive compounds. The phytochemicals shown in Table 1 were present in the aqueous extract of Acacia senegal.Table 1
Phytochemical screening ofAcacia senegal aqueous extract.
Phytochemicals
Present (+); absent (−)
Alkaloids
+
Anthraquinones
−
Carbohydrates
+
Cardiac glycosides
+
Flavonoids
+
Phenols
+
Saponins
+
Steroids
+
Tannins
+
Terpenoids
+


## 2.3. Synthesis of Silver Nanoparticles

10 mL of the obtained leaf extract was added to 90 mL of 1.0 mM AgNO3, and the mixture was autoclaved for 10 minutes at 121°C 15 psi; colour change of the mixture was observed from an initial faint yellow to a brownish-yellow colloidal mixture synthesis. The mixture was washed with methanol twice and finally washed with distilled water several times in a centrifuge at 4400 rpm for 5 min in each cycle. The as-synthesized AgNPs were dried in ambient temperature.


## 2.4. Characterization of Silver Nanoparticles

UV-Vis absorption spectra were recorded on a Varian Cary 50 UV/Vis spectrophotometer. Photoluminescence of the particles was analyzed using a PerkinElmer LS55 Luminescence spectrophotometer. FTIR analysis of the samples was carried out using a PerkinElmer FTIR spectrometer in the range of 4000-450 cm-1. TEM analyses were performed using a JEOL 1400 TEM. Samples were prepared by placing a drop of a dilute solution of nanoparticles on Formvar-coated grids (150-mesh) and allowed to dry completely at room temperature and viewed at an accelerating voltage of 100 kV. X-ray diffraction (XRD) studies were done with a Bruker AXS D8-Advance diffractometer equipped with nickel-filtered Cu Kα radiation (λ=1.5406Å) and the 2θ ranged from 20-80° at 40 kV, 40 mA, and room temperature. A Zeiss Ultra Plus FEG SEM equipped with an Oxford detector EDX at 20 kV which uses Aztec software was used for elemental analysis. The samples were carbon-coated using Quorum coater (Model Q150TE).


## 2.5. Antibacterial Studies of Silver Nanoparticles

### 2.5.1. Zone of Inhibition

The antimicrobial activity of the AgNPs was evaluated using the disc agar diffusion method [42]. Different bacterial strains were grown at 37°C for 24 hours in a 20 mL nutrient broth; microbial cultures were then diluted to 0.5 McFarland standard. Thereafter, standard petri dishes containing Mueller-Hinton agar were inoculated with bacterial culture. Sterile paper disc (6 mm) was impregnated with 10 μL (10 mg/mL in 5% DMSO) of the test extract and placed on the inoculated plates, which were then incubated at 37°C for 24 hours. The next morning, inhibition zones formed around the disc were measured with a transparent ruler in millimetres. This experiment was carried out in triplicates.


### 2.5.2. Minimum Inhibitory Concentration (MIC) Assay

The microplate broth dilution assay was used to assess the minimal inhibitory concentration of theA. senegal plant extract with slight modifications [43]. A 12-hour-old culture was diluted 1 : 100 with freshly prepared Muller-Hinton broth. Thereafter, about 100 μL of extract (10 mg/mL in 5% DMSO) was added to a multiwell plate containing 100 μL of freshly prepared broth and serially diluted. The plates were then incubated overnight at 37°C. Approximately 20 μL of 2 mg/mL freshly prepared iodonitrotetrazolium chloride was added to each well and incubated for 1 hour at the same temperature. The MIC was defined as the lowest concentration of the extract to inhibit bacterial growth.


### 2.5.3. Minimum Bactericidal Concentration (MBC) Assay

A loop full of the microorganism in the wells showing little or no growth in the MIC assay was selected and subcultured on petri plates containing an agar for different microbes. The MBC was defined as the lowest concentration that showed no bacterial growth in the subcultures [44].


### 2.5.4. Lactate Dehydrogenase (LDH) Release Assay

Bacteria-induced cell damage was quantified using the method described by [45]. Wells containing cultured cells were inoculated with 40 μL of Minimum Essential Media (MEM) or tear fluid containing 106 CFU of cytotoxic bacteria/mL. After 3 hours of incubation at 37°C, the supernatant from each well was collected and diluted 1 : 20 with fresh MEM. The quantity of LDH present in the samples was detected by using a cytotoxicity detection kit (Sigma-Aldrich) according to the manufacturer’s instructions and expressed as absorbance at 490 nm. An additional two sets of wells were treated with MEM but without bacteria. One set of cells was used to determine background LDH release, while cells in the other group were lysed with MEM containing Triton X-100 (0.25% v/v) at the end of the assay to determine the amount of LDH released when 100% of the cells were killed.


## 2.5.1. Zone of Inhibition

The antimicrobial activity of the AgNPs was evaluated using the disc agar diffusion method [42]. Different bacterial strains were grown at 37°C for 24 hours in a 20 mL nutrient broth; microbial cultures were then diluted to 0.5 McFarland standard. Thereafter, standard petri dishes containing Mueller-Hinton agar were inoculated with bacterial culture. Sterile paper disc (6 mm) was impregnated with 10 μL (10 mg/mL in 5% DMSO) of the test extract and placed on the inoculated plates, which were then incubated at 37°C for 24 hours. The next morning, inhibition zones formed around the disc were measured with a transparent ruler in millimetres. This experiment was carried out in triplicates.


## 2.5.2. Minimum Inhibitory Concentration (MIC) Assay

The microplate broth dilution assay was used to assess the minimal inhibitory concentration of theA. senegal plant extract with slight modifications [43]. A 12-hour-old culture was diluted 1 : 100 with freshly prepared Muller-Hinton broth. Thereafter, about 100 μL of extract (10 mg/mL in 5% DMSO) was added to a multiwell plate containing 100 μL of freshly prepared broth and serially diluted. The plates were then incubated overnight at 37°C. Approximately 20 μL of 2 mg/mL freshly prepared iodonitrotetrazolium chloride was added to each well and incubated for 1 hour at the same temperature. The MIC was defined as the lowest concentration of the extract to inhibit bacterial growth.


## 2.5.3. Minimum Bactericidal Concentration (MBC) Assay

A loop full of the microorganism in the wells showing little or no growth in the MIC assay was selected and subcultured on petri plates containing an agar for different microbes. The MBC was defined as the lowest concentration that showed no bacterial growth in the subcultures [44].


## 2.5.4. Lactate Dehydrogenase (LDH) Release Assay

Bacteria-induced cell damage was quantified using the method described by [45]. Wells containing cultured cells were inoculated with 40 μL of Minimum Essential Media (MEM) or tear fluid containing 106 CFU of cytotoxic bacteria/mL. After 3 hours of incubation at 37°C, the supernatant from each well was collected and diluted 1 : 20 with fresh MEM. The quantity of LDH present in the samples was detected by using a cytotoxicity detection kit (Sigma-Aldrich) according to the manufacturer’s instructions and expressed as absorbance at 490 nm. An additional two sets of wells were treated with MEM but without bacteria. One set of cells was used to determine background LDH release, while cells in the other group were lysed with MEM containing Triton X-100 (0.25% v/v) at the end of the assay to determine the amount of LDH released when 100% of the cells were killed.


## 3. Results and Discussion

### 3.1. Optical and Structural Properties of As-Synthesized Silver Nanoparticles UsingAcacia senegal Leaf Extract

The biosynthetic approach in the use of naturally occurring reducing agents such as plant extracts, biomass, and biological molecules has emerged as a simple alternative method to complex chemical method of nanoparticle synthesis. Silver nanoparticles have been extensively explored because of their biological and biomedical applications among other functions. Plant extracts are known to contain biomolecules such as phenols, tannins, polysaccharides, saponins, terpenoids, flavonoid, and alkaloids which are well reported to have active reductive potential. Similarly,Acacia senegal leaf extracts are reported to contain compounds like phenols, flavonoid, alkaloid, saponins, tannins, and terpenoids [37]. In this study, the leaves of A. senegal were first boiled, filtered, and added to 1.0 mM solution of AgNO3. Silver ions were reduced to silver nanoparticles by the biomolecules present in the Acacia senegal plant extracts [38, 39, 46]. In this study, the colour change was indicative of the bioreduction of silver ion to AgNPs; thus, the plant extracts act as a reducing agent (Figure 1) [47–49]. Usually, the colour changes can vary from plant to plant and method of synthesis [21, 50]. The biomolecules also act as a stabilizing agent by attaching to the nanoparticles to prevent agglomeration [39].Figure 1
Schematic representation of the biosynthesis of silver nanoparticles fromAcacia senegal leaf extract.The UV-Vis spectrum of the as-synthesized AgNPs is presented in Figure2. The broad peak of the obtained UV-Vis spectrum indicates the presence of well-dispersed particles. The absorption spectrum of Ag nanoparticles revealed a single broad peak at 467 nm of the sample obtained within 24 hours of synthesis, and this corresponds to the Surface Plasmon Resonance (SPR) of Ag nanoparticles as observed in other studies [51, 52]. Additionally, photoluminescence spectrum depicted in Figure 2 showed an emission peak at 437 nm, which is blue shifted when compared to the corresponding absorption spectrum and this corroborates with previous literature [53].Figure 2
UV-Vis absorption and photoluminescence spectra of silver nanoparticles synthesized fromAcacia senegal leaf extract.Figure3 shows representative transmission electron microscopy (TEM) images of silver nanoparticles obtained using A. senegal leaf extract. The images revealed that the as-synthesized silver nanoparticles are loosely aggregated and close to spherical in shape with sizes ranging from 10 nm to 19 nm. The elemental composition of the obtained silver nanoparticles was confirmed by EDX.Figure 3
TEM micrographs of silver nanoparticles synthesized fromA. senegal leaf extract at different magnifications.
(a)
(b)
(c)Powder X-ray diffraction (p-XRD) analysis was used to describe the crystallinity of the as-obtained silver nanoparticles synthesized usingA. senegal leaf extract. The cubic phase (JCPDS card number: 01-087-0719) of silver nanoparticles was confirmed with diffraction peaks at 2θ values of 38.36°, 44.55°, 64.69°, and 77.76° corresponding to (111), (200), (220), and (311) mirror planes (Figure 4). No secondary phase of silver oxide was detected. The sharpness of the XRD patterns or peaks shows that the AgNPs are free of impurities. The crystallite size of 12.82 nm was also calculated using the Scherrer equation; particlesizeS=Bʎ/βcosθ, where S is the size of particles (nm), λ=1.5406Å is a wavelength of the X-ray radiation, B=0.91, β is full width at half maximum (FWHM) of the XRD pattern, and θ is Bragg’s angle in degree [54] and corroborates well with sizes estimated from TEM images (Figure 3).Figure 4
XRD pattern of silver nanoparticles synthesized fromA. senegal leaf extract.FTIR spectra (Figure5) showed the major peaks representing the biomolecules and functional groups present in both the as-synthesized AgNPs and the leaf extract of A. senegal. The FTIR spectrum of the as-synthesized AgNPs (Figure 5 black) shows absorption band peak at 3319 cm-1 which is typical of phenolic compounds known for their role in preventing free radical accumulation in the body [55, 56]. A characteristic band peak is at 1624 -1631 cm-1 typical of N–H, C=O, and C-O functional groups, corresponding to the amide group thus indicating the presence of tannins [55, 56]. The 1404-1414 cm-1 band is indicative of C-C stretch of the aromatic functional group. The peak at 1083 cm-1 indicates the presence of an aliphatic amine of the C-N functional group. The results of the FTIR show that carboxyl (-C=O), hydroxyl (-OH), (C-C) aromatic, and amine (-NH) groups of the leaf extract are probably involved in the reduction and capping of the synthesized AgNPs [57]. Furthermore, the large absorbance peak observed at 3319 cm-1 and the narrow peak at 1404 cm-1 are indicative of the binding of silver ion with the hydroxyl and carboxylate groups of the Acacia senegal extract [58, 59].Figure 5
FTIR spectra ofA. senegal leaf extract (red) and synthesized silver nanoparticles (black) using leaf extract from A. senegal.


### 3.2. Antimicrobial Activity Results of AgNPs on Some Selected Gram-Positive and Gram-Negative Bacteria

The AgNPs obtained from theAcacia senegal aqueous extract had very strong inhibitory action against some selected gram-positive and gram-negative bacteria, and their zone of inhibition (mm) and MIC and MBC in μg/L are presented in Tables 2 and 3. Among the gram-positive bacteria (Table 2), B. cereus had the highest inhibitory activity followed by S. agalactiae, S. aureus, E. faecalis, and E. gallinarum, respectively. MBC values were the lowest in B. cereus while E. faecalis and E. gallinarum were greater than 10; this agrees with the work by Jain and colleagues [3] (Table 2). More so, the % LDH release was the highest in B. cereus, S. aureus, S. agalactiae, and E. hirae, respectively, but was not determined in E. faecalis and E. gallinarum. B. cereus also had the highest MIC and MBC concentration and the highest LDH percentage. The zone of bacteria inhibition for the gram-negative bacteria (Table 3) showed the highest inhibitory activity in E. coli, P. mirabilis, and P. aeruginosa, respectively, and moderate inhibitory activity in K. pneumoniae, A. calcaoceuticals anitratus, and P. vulgaris. S. typhi showed the lowest bacterial inhibition by AgNPs. This result is in agreement with that of Kumar and colleagues [60] who reported that silver nanoparticles were fairly toxic to Pseudomonas aeruginosa while they showed moderate toxicity against P. vulgaris and E. coli but demonstrated low toxicity against S. typhi. Percentage LDH release was the highest in K. pneumoniae and was not determined in all the other organisms except E. coli. AgNPs have different antibacterial effects on the entire tested organism probably due to the difference in the constituent and the degree of thickness of the cell membrane of each bacterium [58]. This determines how the bacteria organism takes up the AgNPs which is a measure of its inhibition zone hence its antibacterial activity. The mechanism of action of silver nanoparticles as an antibacterial agent is not fully understood, but most times it can be determined by various factors including the type of species of the bacteria used, physical surface, chemical properties, and dimension of the AgNPs [39, 58, 61].Table 2
Antimicrobial activity test results of AgNPs on selected gram-positive bacteria.
Bacteria (gram positive)
Zone of inhibition (mm)
MIC
MBC
% LDH release
S. aureus
16.45
±
0.34
1.25
5
33
S. agalactiae
17.22
±
0.28
0.63
2.5
17
B. cereus
18.00
±
1.00
0.31
2.5
58
E. hirae
14.12
±
0.34
1.25
5
7
E. faecalis
16.00
±
1.00
5
>10
ND
E. gallinarum
15.32
±
0.34
2.5
>10
ND
ND: not determined.Table 3
Antimicrobial activity test results of AgNPs on selected gram-negative bacteria.
Bacteria (gram negative)
Zone of inhibition (mm)
MIC
MBC
% LDH
E. coli
17.76
±
1.06
0.63
5
22
P. aeruginosa
15.00
±
1.00
2.5
>10
ND
P. mirabilis
16.33
±
0.57
5
>10
ND
P. vulgaris
11.44
±
0.77
5
>10
ND
K. pneumoniae
13.24
±
0.34
2.5
5
38
A. calcaoceuticals anitratus
11.74
±
0.67
5
>10
ND
S. typhi
10.33
±
0.80
5
>10
ND
ND: not determined.


## 3.1. Optical and Structural Properties of As-Synthesized Silver Nanoparticles UsingAcacia senegal Leaf Extract

The biosynthetic approach in the use of naturally occurring reducing agents such as plant extracts, biomass, and biological molecules has emerged as a simple alternative method to complex chemical method of nanoparticle synthesis. Silver nanoparticles have been extensively explored because of their biological and biomedical applications among other functions. Plant extracts are known to contain biomolecules such as phenols, tannins, polysaccharides, saponins, terpenoids, flavonoid, and alkaloids which are well reported to have active reductive potential. Similarly,Acacia senegal leaf extracts are reported to contain compounds like phenols, flavonoid, alkaloid, saponins, tannins, and terpenoids [37]. In this study, the leaves of A. senegal were first boiled, filtered, and added to 1.0 mM solution of AgNO3. Silver ions were reduced to silver nanoparticles by the biomolecules present in the Acacia senegal plant extracts [38, 39, 46]. In this study, the colour change was indicative of the bioreduction of silver ion to AgNPs; thus, the plant extracts act as a reducing agent (Figure 1) [47–49]. Usually, the colour changes can vary from plant to plant and method of synthesis [21, 50]. The biomolecules also act as a stabilizing agent by attaching to the nanoparticles to prevent agglomeration [39].Figure 1
Schematic representation of the biosynthesis of silver nanoparticles fromAcacia senegal leaf extract.The UV-Vis spectrum of the as-synthesized AgNPs is presented in Figure2. The broad peak of the obtained UV-Vis spectrum indicates the presence of well-dispersed particles. The absorption spectrum of Ag nanoparticles revealed a single broad peak at 467 nm of the sample obtained within 24 hours of synthesis, and this corresponds to the Surface Plasmon Resonance (SPR) of Ag nanoparticles as observed in other studies [51, 52]. Additionally, photoluminescence spectrum depicted in Figure 2 showed an emission peak at 437 nm, which is blue shifted when compared to the corresponding absorption spectrum and this corroborates with previous literature [53].Figure 2
UV-Vis absorption and photoluminescence spectra of silver nanoparticles synthesized fromAcacia senegal leaf extract.Figure3 shows representative transmission electron microscopy (TEM) images of silver nanoparticles obtained using A. senegal leaf extract. The images revealed that the as-synthesized silver nanoparticles are loosely aggregated and close to spherical in shape with sizes ranging from 10 nm to 19 nm. The elemental composition of the obtained silver nanoparticles was confirmed by EDX.Figure 3
TEM micrographs of silver nanoparticles synthesized fromA. senegal leaf extract at different magnifications.
(a)
(b)
(c)Powder X-ray diffraction (p-XRD) analysis was used to describe the crystallinity of the as-obtained silver nanoparticles synthesized usingA. senegal leaf extract. The cubic phase (JCPDS card number: 01-087-0719) of silver nanoparticles was confirmed with diffraction peaks at 2θ values of 38.36°, 44.55°, 64.69°, and 77.76° corresponding to (111), (200), (220), and (311) mirror planes (Figure 4). No secondary phase of silver oxide was detected. The sharpness of the XRD patterns or peaks shows that the AgNPs are free of impurities. The crystallite size of 12.82 nm was also calculated using the Scherrer equation; particlesizeS=Bʎ/βcosθ, where S is the size of particles (nm), λ=1.5406Å is a wavelength of the X-ray radiation, B=0.91, β is full width at half maximum (FWHM) of the XRD pattern, and θ is Bragg’s angle in degree [54] and corroborates well with sizes estimated from TEM images (Figure 3).Figure 4
XRD pattern of silver nanoparticles synthesized fromA. senegal leaf extract.FTIR spectra (Figure5) showed the major peaks representing the biomolecules and functional groups present in both the as-synthesized AgNPs and the leaf extract of A. senegal. The FTIR spectrum of the as-synthesized AgNPs (Figure 5 black) shows absorption band peak at 3319 cm-1 which is typical of phenolic compounds known for their role in preventing free radical accumulation in the body [55, 56]. A characteristic band peak is at 1624 -1631 cm-1 typical of N–H, C=O, and C-O functional groups, corresponding to the amide group thus indicating the presence of tannins [55, 56]. The 1404-1414 cm-1 band is indicative of C-C stretch of the aromatic functional group. The peak at 1083 cm-1 indicates the presence of an aliphatic amine of the C-N functional group. The results of the FTIR show that carboxyl (-C=O), hydroxyl (-OH), (C-C) aromatic, and amine (-NH) groups of the leaf extract are probably involved in the reduction and capping of the synthesized AgNPs [57]. Furthermore, the large absorbance peak observed at 3319 cm-1 and the narrow peak at 1404 cm-1 are indicative of the binding of silver ion with the hydroxyl and carboxylate groups of the Acacia senegal extract [58, 59].Figure 5
FTIR spectra ofA. senegal leaf extract (red) and synthesized silver nanoparticles (black) using leaf extract from A. senegal.


## 3.2. Antimicrobial Activity Results of AgNPs on Some Selected Gram-Positive and Gram-Negative Bacteria

The AgNPs obtained from theAcacia senegal aqueous extract had very strong inhibitory action against some selected gram-positive and gram-negative bacteria, and their zone of inhibition (mm) and MIC and MBC in μg/L are presented in Tables 2 and 3. Among the gram-positive bacteria (Table 2), B. cereus had the highest inhibitory activity followed by S. agalactiae, S. aureus, E. faecalis, and E. gallinarum, respectively. MBC values were the lowest in B. cereus while E. faecalis and E. gallinarum were greater than 10; this agrees with the work by Jain and colleagues [3] (Table 2). More so, the % LDH release was the highest in B. cereus, S. aureus, S. agalactiae, and E. hirae, respectively, but was not determined in E. faecalis and E. gallinarum. B. cereus also had the highest MIC and MBC concentration and the highest LDH percentage. The zone of bacteria inhibition for the gram-negative bacteria (Table 3) showed the highest inhibitory activity in E. coli, P. mirabilis, and P. aeruginosa, respectively, and moderate inhibitory activity in K. pneumoniae, A. calcaoceuticals anitratus, and P. vulgaris. S. typhi showed the lowest bacterial inhibition by AgNPs. This result is in agreement with that of Kumar and colleagues [60] who reported that silver nanoparticles were fairly toxic to Pseudomonas aeruginosa while they showed moderate toxicity against P. vulgaris and E. coli but demonstrated low toxicity against S. typhi. Percentage LDH release was the highest in K. pneumoniae and was not determined in all the other organisms except E. coli. AgNPs have different antibacterial effects on the entire tested organism probably due to the difference in the constituent and the degree of thickness of the cell membrane of each bacterium [58]. This determines how the bacteria organism takes up the AgNPs which is a measure of its inhibition zone hence its antibacterial activity. The mechanism of action of silver nanoparticles as an antibacterial agent is not fully understood, but most times it can be determined by various factors including the type of species of the bacteria used, physical surface, chemical properties, and dimension of the AgNPs [39, 58, 61].Table 2
Antimicrobial activity test results of AgNPs on selected gram-positive bacteria.
Bacteria (gram positive)
Zone of inhibition (mm)
MIC
MBC
% LDH release
S. aureus
16.45
±
0.34
1.25
5
33
S. agalactiae
17.22
±
0.28
0.63
2.5
17
B. cereus
18.00
±
1.00
0.31
2.5
58
E. hirae
14.12
±
0.34
1.25
5
7
E. faecalis
16.00
±
1.00
5
>10
ND
E. gallinarum
15.32
±
0.34
2.5
>10
ND
ND: not determined.Table 3
Antimicrobial activity test results of AgNPs on selected gram-negative bacteria.
Bacteria (gram negative)
Zone of inhibition (mm)
MIC
MBC
% LDH
E. coli
17.76
±
1.06
0.63
5
22
P. aeruginosa
15.00
±
1.00
2.5
>10
ND
P. mirabilis
16.33
±
0.57
5
>10
ND
P. vulgaris
11.44
±
0.77
5
>10
ND
K. pneumoniae
13.24
±
0.34
2.5
5
38
A. calcaoceuticals anitratus
11.74
±
0.67
5
>10
ND
S. typhi
10.33
±
0.80
5
>10
ND
ND: not determined.


## 4. Conclusion

In this study, silver nanoparticles were successfully synthesized usingA. senegal aqueous leaf extract from the bioreduction of silver nitrate solution. The synthesized silver nanoparticles were confirmed and characterized using UV-Vis and TEM analysis while X-ray diffraction pattern confirmed the formation of the cubic phase of AgNPs. The obtained AgNPs displayed dispersion of the particles with sizes in the range of 10-19 nm, relatively small-sized particles enough for antibacterial testing. FTIR spectrum analysis of both AgNPs and leaf extract showed prominent broad band peaks that are probably responsible for effective capping and stabilizing of the silver nanoparticles. Antimicrobial studies using AgNPs showed that the nanoparticles have antibacterial activities, especially on the gram-positive bacteria. This present study established an easy, quick, and economical method to synthesize silver nanoparticles from A. senegal leaf extract, and this method could easily allow for industrial scale-up production of similar or other metallic nanoparticles that can be employed as a bactericidal agent in various biological applications.



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*Source: 6474913-2020-11-29.xml*