# Silica Sol-Gel Entrapment of the Enzyme Chloroperoxidase

Authors: Tuan Le; Selina Chan; Bassem Ebaid; Monika Sommerhalter
Journal: Journal of Nanotechnology
 (2015)

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## Abstract

The enzyme chloroperoxidase (CPO) was immobilized in silica sol-gel beads prepared from tetramethoxysilane. The average pore diameter of the silica host structure (~3 nm) was smaller than the globular CPO diameter (~6 nm) and the enzyme remained entrapped after sol-gel maturation. The catalytic performance of the entrapped enzyme was assessed via the pyrogallol peroxidation reaction. Sol-gel beads loaded with 4μg CPO per mL sol solution reached 9–12% relative activity compared to free CPO in solution. Enzyme kinetic analysis revealed a decrease in kcat but no changes in KM or KI. Product release or enzyme damage might thus limit catalytic performance. Yet circular dichroism and visible absorption spectra of transparent CPO sol-gel sheets did not indicate enzyme damage. Activity decline due to methanol exposure was shown to be reversible in solution. To improve catalytic performance the sol-gel protocol was modified. The incorporation of 5, 20, or 40% methyltrimethoxysilane resulted in more brittle sol-gel beads but the catalytic performance increased to 14% relative to free CPO in solution. The use of more acidic casting buffers (pH 4.5 or 5.5 instead of 6.5) resulted in a more porous silica host reaching up to 18% relative activity.


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## Body

## 1. Introduction

Silica nanostructures can be fabricated using a room temperature sol-gel process that is compatible with biomolecules [1, 2]. The entrapment of enzymes inside these silica nanostructures has facilitated diverse applications in biocatalysis and biosensing [3]. Here, we demonstrate the use of sol-gel technology to make a biocatalyst based on entrapment of the enzyme chloroperoxidase (CPO) inside a silica nanostructure.CPO (EC 1.11.1.10) is one of the most versatile heme enzymes known to date. It can be obtained as a secreted, glycosylated protein from the marine fungusCaldariomyces fumago [4, 5]. CPO catalyzes halogenation, sulfoxidation, hydroxylation, and epoxidation reactions with high substrate promiscuity under environmentally benign conditions [6]. CPO catalyzed oxidative transformations are based on hydrogen peroxide or an alkyl peroxide as oxidant, whereas the equivalent traditional chemical reactions require stoichiometric amounts of heavy metal salts [7]. Another advantageous feature of CPO is its ability to perform these reactions in a highly enantioselective manner [8]. Transforming CPO into a practical biocatalyst for oxidative transformations in biosynthetic chemistry is therefore highly desirable. Further possible biotechnological applications of CPO include wastewater treatment or upgrading petroleum products [9–11]. The use of CPO as biocatalyst, however, is hampered by its loss of activity in the presence of organic solvents, deactivation at high concentrations of the oxidant H2O2, and instability at elevated temperatures [8, 12].Various approaches have been attempted to improve the stability and productivity of CPO in solution [13–16] as well as in immobilized form [12, 17–21]. In our study, CPO was entrapped in a hydrogel prepared from the precursor tetramethoxysilane (TMOS) with or without the addition of methyltrimethoxysilane (MTMS). Enzyme immobilization offers key advantages, most notably ease of enzyme recovery and reusability [22]. Silica-based sol-gels furnish chemically inert and optically transparent immobilization matrices [2, 23]. However, sol-gel immobilization is often accompanied by a loss of enzymatic activity. This loss can be due to enzyme damage, leakage of enzyme from the immobilization host, or limited material transport of substrates and products within the sol-gel nanostructure. We examined each of these possible reasons using absorption and circular dichroism spectroscopy, porosimetry, and an enzyme activity assay for CPO, that is, the peroxidation of pyrogallol. The effect of methanol on CPO was also investigated since methanol is released from the precursor TMOS during the hydrolysis and condensation reactions of the sol-gel process. In our attempts to optimize the catalytic performance of sol-gel immobilized CPO, we exploited the stability of CPO in acidic solutions [24] and deviated from the pH range of 6.0–7.0 which is typically employed in protein sol-gel immobilization procedures. The use of more acidic solutions in the sol-gel process might also be beneficial for various sol-gel applications based on other proteins that either withstand or thrive in acidic conditions.


## 2. Materials and Methods

### 2.1. Materials

CPO fromC. fumago was purchased from Sigma-Aldrich. The turbid suspensions were centrifuged at 15,000 rpm for 5 minutes in an Eppendorf microcentrifuge. The concentrations of the CPO supernatants were determined via the intensity of the Soret band [25] and confirmed with protein assays [26]. The CPO samples used in this study had concentrations in the range of 9 to 15 mg/mL. All other reagents were purchased from Sigma-Aldrich or Fisher Scientific. Aqueous solutions were prepared with deionized water obtained from a Millipore Milli-Q device.


### 2.2. Sol-Gel Entrapment

A sol solution was prepared by mixing 50μL deionized water, 4.7 μL 0.010 M hydrochloric acid, and 235 μL TMOS in a microcentrifuge tube. If MTMS was included, TMOS and MTMS were premixed. The molar ratio of MTMS was either 5, 20, or 40% of the total MTMS and TMOS precursor mixture. The sol solution was sonicated in a Branson 1510 sonicator bath filled with ice water for 30 minutes and either used immediately thereafter or stored for up to a week at 4°C. In a microcentrifuge tube, 140 μL sol, 480 μL casting buffer, and 500 μL diluted CPO solution were mixed. The casting buffers were prepared by mixing 0.1 M citric acid and 0.2 M dibasic sodium phosphate to reach pH values of 4.5, 5.5, 6.0, or 6.5. CPO was diluted in 20 mM potassium phosphate-potassium hydroxide buffer, pH 6.0. Small beads of 50 μL volume were prepared by pipetting the mixture onto parafilm sheets. As the pH value of the casting buffer decreased the gelation time increased from approximately 15 minutes (pH 6.5) to 240 min (pH 4.5). The CPO sol-gel beads were transferred into glass tubes in sets of three to eight beads and covered with a storage buffer composed of 2.1 mL deionized water and 0.32 mL 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2. This storage buffer was typically exchanged with a new portion of storage buffer after one hour, and thereafter each day for seven or ten days. The storage temperature was 4°C.


### 2.3. Activity Assays

Activity assays were typically conducted in 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2 with 2.7 or 8.6 mM H2O2, 35 mM pyrogallol, and 0.2 μg/mL CPO. CPO was added last to start the reaction. The assay volume was 3 mL. Control reactions without the enzyme were performed in parallel. The hydrogen peroxide and pyrogallol solutions were prepared fresh on the day of the measurements and pyrogallol was kept in the dark. The absorbance increase at 420 nm was monitored every 3 seconds for 2 minutes using a NanoDrop 2000c (Thermo Fisher Scientific) with cuvette option. A molar absorptivity value of 2640 M−1cm−1 for the product purpurogallin was used to calculate the enzyme activity [24]. One International Unit (IU) corresponds to the formation of one micromole purpurogallin in one minute at room temperature and pH 4.2.For enzyme kinetic studies, the concentrations of the substrates pyrogallol and H2O2 were varied. The substrate inhibition model described by (1) was used to fit the dependence of the initial reaction velocity, v0, on the substrate concentration, [S]. The fit parameters are the Michaelis-Menten constant, KM, the substrate inhibition constant, KI, and the maximum initial reaction velocity, vmax. The parameter vmax depends on the turnover number, kcat, and the enzyme concentration, [E]. The fit was carried out with the program EnzFitter from Biosoft:(1)v0=vmax·SKM+S+S2/KIwithvmax=kcat·E.To detect how much CPO leaked from sol-gel beads, activity assays were conducted with the storage buffers that were removed and replaced during the maturation phase of the sol-gel beads. These assays were conducted in a similar manner as described above with the following exception: the assay was started with the addition of H2O2 as no extra CPO was added.Activity assays with CPO sol-gel beads were performed with longer time intervals of 20 or 30 seconds for a total duration of 5 minutes. A Milton Roy Spectronic 20D instrument was used to monitor the absorbance at 420 nm. Agitation of the reaction solution and the CPO sol-gel beads via inversion or pipetting was crucial to ensure good mixing in between absorbance recordings. Control reactions with sol-gel beads that did not contain CPO confirmed that the sol-gel beads themselves do not catalyze the peroxidation of pyrogallol.


### 2.4. Spectroscopic Studies (CD and UV/VIS)

CD spectra were recorded with an AVIV Model 215 circular dichroism spectrometer at 25°C. The temperature was controlled with a thermostat. We employed the sample preparation method developed by Eggers and Valentine [27]. A buffer of low concentration (2 mM sodium phosphate, pH 7.0) was chosen to minimize total sample absorbance in the UV region. To achieve efficient gelation despite the low concentration of the buffer, a pH value of 7.0 was selected. A sol solution was prepared by sonicating 7.37 mL TMOS, 0.92 mL dH2O, and 0.88 mL 0.01 M hydrochloric acid for 20 minutes in an ice-water bath. A portion of 1.75 mL of this sol-gel solution was gently mixed with 2.625 mL CPO solution containing 12 μM CPO in 2 mM sodium phosphate, pH 7.0, and cast into a plastic cassette of 1 mm thickness. The cassettes were purchased from Life Technologies. For the first four days, the storage buffer on top of the sol-gel sheet was exchanged daily. For complete maturation inside the more enclosed cassettes, the CPO sol-gel sheets were stored for one month at 4°C. Prior to the actual CD measurements, the cassette housing the CPO sol-gel was opened. A small piece was cut out with a razor blade and transferred into a CD quartz cuvette of 2 mm thickness. The remaining space was filled with 2 mM sodium phosphate buffer, pH 7.0.UV/VIS spectra were recorded using a UV mini 1240 Shimadzu single beam spectrophotometer. The CPO sol-gel material was prepared as described in Section2.2. However, instead of casting beads, the solution was poured into 1 mm thick plastic cassettes and a higher CPO concentration yielding 1.6 mg/mL CPO in the final sol-gel sheet was used. The CPO sol-gel sheet was stored in the cassette for one month at 4°C. A rectangular piece was cut out and placed onto one wall of a plastic cuvette of 10 mm thickness. The CPO sol-gel sheet remained attached to the plastic wall after the cuvette was filled with 50 mM potassium phosphate buffer, pH 6.0. To test the effect of changing the pH value of buffers surrounding the CPO sol-gel sheets, the molarity of the potassium phosphate buffers at various pH values was increased to 0.5 M.


### 2.5. Influence of Methanol on CPO’s Catalytic Activity and Spectroscopic Properties

CPO dissolved at concentrations of up to 9μM in 0.1 M citrate-0.2 M phosphate buffer, pH 4.2 was incubated with 11% v/v methanol. After specific time intervals, sample aliquots were removed to record UV/VIS or CD spectra or to measure the enzymatic activity via the pyrogallol peroxidation assay. Reference samples without methanol were prepared in parallel. To test whether any effects caused by methanol exposure might be reversible methanol treated samples were dialyzed for three hours in a Pierce Slide-A-Lyzer (10,000 MWCO) against 1 L of 0.01 M citrate-0.02 M phosphate buffer, pH 4.2 at room temperature.


### 2.6. Porosimetry Measurements

All porosimetry measurements were conducted with an ASAP 2020 physisorption analyzer from Micromeritics using nitrogen gas. To prepare the samples, the solvent exchange procedure described by Harreld and coworkers was used [28]. The sol-gel beads (typically 8) were placed in ~4 mL acetone overnight. The acetone was exchanged each day for three days. For the final two days, n-pentane was used instead of acetone. N-pentane and acetone are miscible with each other, but n-pentane evaporates more readily than acetone. This technique yields dry beads while minimizing the risk of pore collapse. The beads were placed in the sample degas station of the physisorption analyzer for approximately 8 hours at 37°C until constant pressure was reached. Nitrogen adsorption and desorption curves were recorded and analyzed with the Brunauer-Emmett-Teller (BET) algorithm. Proper performance of the instrument was confirmed with a silica-alumina standard provided by Micromeritics.


## 2.1. Materials

CPO fromC. fumago was purchased from Sigma-Aldrich. The turbid suspensions were centrifuged at 15,000 rpm for 5 minutes in an Eppendorf microcentrifuge. The concentrations of the CPO supernatants were determined via the intensity of the Soret band [25] and confirmed with protein assays [26]. The CPO samples used in this study had concentrations in the range of 9 to 15 mg/mL. All other reagents were purchased from Sigma-Aldrich or Fisher Scientific. Aqueous solutions were prepared with deionized water obtained from a Millipore Milli-Q device.


## 2.2. Sol-Gel Entrapment

A sol solution was prepared by mixing 50μL deionized water, 4.7 μL 0.010 M hydrochloric acid, and 235 μL TMOS in a microcentrifuge tube. If MTMS was included, TMOS and MTMS were premixed. The molar ratio of MTMS was either 5, 20, or 40% of the total MTMS and TMOS precursor mixture. The sol solution was sonicated in a Branson 1510 sonicator bath filled with ice water for 30 minutes and either used immediately thereafter or stored for up to a week at 4°C. In a microcentrifuge tube, 140 μL sol, 480 μL casting buffer, and 500 μL diluted CPO solution were mixed. The casting buffers were prepared by mixing 0.1 M citric acid and 0.2 M dibasic sodium phosphate to reach pH values of 4.5, 5.5, 6.0, or 6.5. CPO was diluted in 20 mM potassium phosphate-potassium hydroxide buffer, pH 6.0. Small beads of 50 μL volume were prepared by pipetting the mixture onto parafilm sheets. As the pH value of the casting buffer decreased the gelation time increased from approximately 15 minutes (pH 6.5) to 240 min (pH 4.5). The CPO sol-gel beads were transferred into glass tubes in sets of three to eight beads and covered with a storage buffer composed of 2.1 mL deionized water and 0.32 mL 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2. This storage buffer was typically exchanged with a new portion of storage buffer after one hour, and thereafter each day for seven or ten days. The storage temperature was 4°C.


## 2.3. Activity Assays

Activity assays were typically conducted in 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2 with 2.7 or 8.6 mM H2O2, 35 mM pyrogallol, and 0.2 μg/mL CPO. CPO was added last to start the reaction. The assay volume was 3 mL. Control reactions without the enzyme were performed in parallel. The hydrogen peroxide and pyrogallol solutions were prepared fresh on the day of the measurements and pyrogallol was kept in the dark. The absorbance increase at 420 nm was monitored every 3 seconds for 2 minutes using a NanoDrop 2000c (Thermo Fisher Scientific) with cuvette option. A molar absorptivity value of 2640 M−1cm−1 for the product purpurogallin was used to calculate the enzyme activity [24]. One International Unit (IU) corresponds to the formation of one micromole purpurogallin in one minute at room temperature and pH 4.2.For enzyme kinetic studies, the concentrations of the substrates pyrogallol and H2O2 were varied. The substrate inhibition model described by (1) was used to fit the dependence of the initial reaction velocity, v0, on the substrate concentration, [S]. The fit parameters are the Michaelis-Menten constant, KM, the substrate inhibition constant, KI, and the maximum initial reaction velocity, vmax. The parameter vmax depends on the turnover number, kcat, and the enzyme concentration, [E]. The fit was carried out with the program EnzFitter from Biosoft:(1)v0=vmax·SKM+S+S2/KIwithvmax=kcat·E.To detect how much CPO leaked from sol-gel beads, activity assays were conducted with the storage buffers that were removed and replaced during the maturation phase of the sol-gel beads. These assays were conducted in a similar manner as described above with the following exception: the assay was started with the addition of H2O2 as no extra CPO was added.Activity assays with CPO sol-gel beads were performed with longer time intervals of 20 or 30 seconds for a total duration of 5 minutes. A Milton Roy Spectronic 20D instrument was used to monitor the absorbance at 420 nm. Agitation of the reaction solution and the CPO sol-gel beads via inversion or pipetting was crucial to ensure good mixing in between absorbance recordings. Control reactions with sol-gel beads that did not contain CPO confirmed that the sol-gel beads themselves do not catalyze the peroxidation of pyrogallol.


## 2.4. Spectroscopic Studies (CD and UV/VIS)

CD spectra were recorded with an AVIV Model 215 circular dichroism spectrometer at 25°C. The temperature was controlled with a thermostat. We employed the sample preparation method developed by Eggers and Valentine [27]. A buffer of low concentration (2 mM sodium phosphate, pH 7.0) was chosen to minimize total sample absorbance in the UV region. To achieve efficient gelation despite the low concentration of the buffer, a pH value of 7.0 was selected. A sol solution was prepared by sonicating 7.37 mL TMOS, 0.92 mL dH2O, and 0.88 mL 0.01 M hydrochloric acid for 20 minutes in an ice-water bath. A portion of 1.75 mL of this sol-gel solution was gently mixed with 2.625 mL CPO solution containing 12 μM CPO in 2 mM sodium phosphate, pH 7.0, and cast into a plastic cassette of 1 mm thickness. The cassettes were purchased from Life Technologies. For the first four days, the storage buffer on top of the sol-gel sheet was exchanged daily. For complete maturation inside the more enclosed cassettes, the CPO sol-gel sheets were stored for one month at 4°C. Prior to the actual CD measurements, the cassette housing the CPO sol-gel was opened. A small piece was cut out with a razor blade and transferred into a CD quartz cuvette of 2 mm thickness. The remaining space was filled with 2 mM sodium phosphate buffer, pH 7.0.UV/VIS spectra were recorded using a UV mini 1240 Shimadzu single beam spectrophotometer. The CPO sol-gel material was prepared as described in Section2.2. However, instead of casting beads, the solution was poured into 1 mm thick plastic cassettes and a higher CPO concentration yielding 1.6 mg/mL CPO in the final sol-gel sheet was used. The CPO sol-gel sheet was stored in the cassette for one month at 4°C. A rectangular piece was cut out and placed onto one wall of a plastic cuvette of 10 mm thickness. The CPO sol-gel sheet remained attached to the plastic wall after the cuvette was filled with 50 mM potassium phosphate buffer, pH 6.0. To test the effect of changing the pH value of buffers surrounding the CPO sol-gel sheets, the molarity of the potassium phosphate buffers at various pH values was increased to 0.5 M.


## 2.5. Influence of Methanol on CPO’s Catalytic Activity and Spectroscopic Properties

CPO dissolved at concentrations of up to 9μM in 0.1 M citrate-0.2 M phosphate buffer, pH 4.2 was incubated with 11% v/v methanol. After specific time intervals, sample aliquots were removed to record UV/VIS or CD spectra or to measure the enzymatic activity via the pyrogallol peroxidation assay. Reference samples without methanol were prepared in parallel. To test whether any effects caused by methanol exposure might be reversible methanol treated samples were dialyzed for three hours in a Pierce Slide-A-Lyzer (10,000 MWCO) against 1 L of 0.01 M citrate-0.02 M phosphate buffer, pH 4.2 at room temperature.


## 2.6. Porosimetry Measurements

All porosimetry measurements were conducted with an ASAP 2020 physisorption analyzer from Micromeritics using nitrogen gas. To prepare the samples, the solvent exchange procedure described by Harreld and coworkers was used [28]. The sol-gel beads (typically 8) were placed in ~4 mL acetone overnight. The acetone was exchanged each day for three days. For the final two days, n-pentane was used instead of acetone. N-pentane and acetone are miscible with each other, but n-pentane evaporates more readily than acetone. This technique yields dry beads while minimizing the risk of pore collapse. The beads were placed in the sample degas station of the physisorption analyzer for approximately 8 hours at 37°C until constant pressure was reached. Nitrogen adsorption and desorption curves were recorded and analyzed with the Brunauer-Emmett-Teller (BET) algorithm. Proper performance of the instrument was confirmed with a silica-alumina standard provided by Micromeritics.


## 3. Results and Discussion

### 3.1. Peroxidation of Pyrogallol Catalyzed by Free and Sol-Gel Entrapped CPO

The catalytic activity of CPO was assessed by monitoring the peroxidation reaction of pyrogallol. To determine optimal reaction conditions, we varied the pH value of the assay buffer and the concentrations of pyrogallol and hydrogen peroxide. Our observations for free CPO agree well with previous studies [24, 29]. Acidic conditions, with pH values in the range of 3.5 to 4.5, and pyrogallol concentrations at approximately 35 mM resulted in optimum catalytic performance. Very high pyrogallol concentrations gave rise to substrate inactivation, but the enzyme was even more sensitive towards inactivation by the cosubstrate H2O2. The H2O2 concentration should not exceed 10 mM. The enzyme kinetic data obtained for sol-gel entrapped CPO displayed similar features albeit at much lower specific activity. Notably, sol-gel entrapment did not abolish the detrimental effect of H2O2. All data displayed in Figure 1 was thus fitted with a substrate inhibition model (see (1)).Figure 1
Peroxidation of pyrogallol catalyzed by free CPO (open squares; 0.575μg CPO in 3 mL total assay volume) and sol-gel entrapped CPO (grey circles; 0.575 μg CPO entrapped in three sol-gel beads placed in 3 mL total assay volume). The CPO sol-gel beads were prepared from TMOS with a pH 6.0 casting buffer and matured for one week with a buffer exchange on every other day. The activity assays were performed in 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2 with constant concentrations of either 2.7 mM H2O2 (a) or 35 mM pyrogallol (b). All data were measured in triplicate and are displayed as mean values ± one standard deviation. The parameters of the curve fits are summarized in Table 1.
(a)
(b)Table 1
Enzyme kinetic parameters for the CPO catalyzed peroxidation of pyrogallol∗.
K
M (mM)
K
I (mM)
v
max
⁡ (IU/mg)
k
cat (1/sec)
Pyrogallol
Free CPO (R2 = 0.983)
11 ± 1.0
160  ± 20
2000 ± 80
1400 ± 60
Sol-gel entrapped CPO (R2 = 0.847)
9.3 ± 2.6
190 ± 74
100 ± 12
70 ± 8.4
H2O2
Free CPO (R2 = 0.921)
17 ± 6.2
6.4 ± 2.2
9100 ± 2500
6370 ± 1750
Sol-gel entrapped CPO (R2 = 0.657)
27 ± 39
6.1 ± 8.6
680 ± 830
480 ± 580
∗Enzyme kinetic parameters were obtained by fitting the data shown in Figure 1(a) (pyrogallol) and Figure 1(b) (H2O2) to a substrate inhibition model (see (1)). All parameters are presented as value of the fit ± one standard error of the fit.The enzyme kinetic parameters for the peroxidation of pyrogallol catalyzed by free and sol-gel entrapped CPO are summarized in Table1. Significantly smaller vmax and thus kcat values were obtained for sol-gel entrapped CPO compared to free CPO. The parameters Km and KI did not change significantly in response to sol-gel entrapment for either substrate. The poor quality of the fit shown in Figure 1(b) for the data on sol-gel entrapped CPO might be caused by large data point variations and the limitations of the substrate inhibition model. Manoj et al. [29] demonstrated that the substrate inhibition model used here (see (1)) can yield acceptable fits for individual substrate variations, but global fit parameters were shown to be unattainable [29]. For example, the KM and KI values of one substrate depended on the concentration of the other cosubstrate. All kinetic parameters determined here are thus only apparent values and the parameters for sol-gel entrapped CPO are further influenced by additional factors. For example, kcat was calculated based on the assumption that all entrapped CPO molecules are able to participate in the catalytic process. This will not be the case for CPO molecules that are entrapped in closed silica pores or are otherwise permanently shielded and for CPO molecules with compromised functionality due to enzyme damage. The decrease in kcat (and also vmax) might thus signify an apparent decrease in accessible and/or active CPO molecules upon sol-gel entrapment. Notably, hindered product release can also cause a decrease in kcat. The kcat value is either determined by the rate of catalytic turnover or the rate of product release, depending on which of the two steps is slower and thus rate-limiting. The silica nanostructure does not seem to impose any significant diffusional constraints on the molecules pyrogallol and possibly H2O2. If these substrate molecules were encountering substantial diffusional barriers, an increase in their apparent KM and KI values would be expected.


### 3.2. Entrapment and Catalytic Performance of CPO in Sol-Gel Beads

To further assess the entrapment and catalytic performance of the immobilized enzyme, different amounts of CPO were immobilized inside sol-gel beads corresponding to final concentrations of 40μg/mL, 8 μg/mL, and 4 μg/mL per total sol-gel solution. The storage buffer was exchanged to remove methanol originating from the hydrolysis and condensation steps of the sol-gel formation. CPO activity was measured in these exchanged buffer samples and compared to a reference sample with free CPO in solution to quantify enzyme leakage (see Figure 2). Higher enzyme loading resulted in more enzyme leakage. As the sol-gel material matured and more connections formed within the silica mesh, enzyme leakage declined.Figure 2
Three sets of CPO sol-gel beads with CPO concentrations of 40μg/mL (dark grey bars), 8 μg/mL (grey bars), and 4 μg/mL (light grey bars) were aged for one week. The storage buffer was replaced in the time intervals indicated on the graph and tested for CPO leakage. Each set was replicated three times and contained three beads per sample tube. The specific activity of the reference sample with free CPO in solution was 1436±39 IU/mg. The column heights represent the mean value and the error bars represent ± one standard deviation.Table2 contains the results of the activity measurements with the CPO sol-gel beads and summarizes the cumulative leakage over the first six days. The activity measurements were performed with CPO sol-gel beads that were seven days old. Each assay comprised 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2, 2.7 mM H2O2, and 35 mM pyrogallol. The CPO sol-gel beads with the highest CPO loading of 40 μg CPO per mL sol-gel yielded the highest absolute activity values of 249±33 mIU. A comparison among specific activity values reported per mg of initially loaded CPO, however, clearly showed that the CPO sol-gel beads with a lower enzyme content of 8 or 4 μg/mL performed better. At a higher loading of CPO, more CPO molecules might be obstructed by either other enzyme molecules or the silica nanostructure. Also, some of these CPO molecules might be entrapped in closed pores.Table 2
Catalytic performance of sol-gel beads loaded with different CPO amounts.
CPO loading of sol-gel beads (µg/mL)
40
8
4
Activity (mIU)∗
249 ± 33
138 ± 18
76 ± 4
Specific activity (IU/mg)∗
41 ± 6
115 ± 15
127 ± 6
Relative activity compared to free CPO (%)#
2.9
8.0
8.80
Cumulative leakage for six days (%)†
15
12
7
∗Absolute activity values in mIU (×10−3 International Units) and specific activity values per mg initially loaded CPO are presented as mean values ± one standard deviation. All sample sets were prepared in triplicate. #The relative activity was based on a reference assay with free CPO in solution. The reference value was 1436 ± 39 IU/mg. †The cumulative leakage over six days of maturation corresponds to the summation of the leakage data shown in Figure 2.Another set of three tubes each containing three beads loaded with 4μg/mL CPO was aged for one week and tested for reusability (see Figure 3). The CPO sol-gel beads can be reused up to three times in a convenient manner by replacing the liquid phase composed of buffer and product molecules with new buffer and substrate. However, a further decline in catalytic performance was apparent. We also noticed that all CPO sol-gel beads adopted the yellow-orange color of the product purpurogallin after the first use. Gentle washing with the storage buffer diminished the coloration only slightly. Thus, clogging of the sol-gel nanostructure with product molecules was one factor that hampered the reusability of the CPO sol-gel beads. Furthermore, the peroxidation reactions catalyzed by CPO are prone to substrate inhibition (see Figure 1). Trapped pyrogallol and H2O2 molecules could therefore interfere with effective reuse. Jung and Hartmann demonstrated thatin situ generation of hydrogen peroxide via coimmobilization of glucose oxidase can improve the reusability of cross-linked CPO molecules entrapped in mesoporous molecular sieves [30].Figure 3
Reusability test for CPO sol-gel beads loaded with 4μg/mL CPO. Three sets, each with three beads per tube, were used in this test. The column heights represent the mean specific activity value and the error bars represent ± one standard deviation. The first measurement was taken after the beads were aged for one week with daily buffer exchanges. The number of days that passed before the next reuse is listed on the x-axis.The specific activity of three independently prepared sets of sol-gel beads all containing ~4μg/mL CPO was different: 70±15 IU/mg (Figure 1), 127±6 IU/mg (Table 2), and 171±14 IU/mg (Figure 3). The CPO sol-gel materials used for generating the data presented in Table 2 and Figure 3 were prepared from the same CPO vial with a free CPO reference value of 1436±39 IU/mg. The reference value for the first CPO vial was only 1280±80 IU/mg. However, many other experimental parameters, such as the exact sol-gel composition, the quality of all starting materials, humidity, temperature, and duration of sol-gel drying phase, can also influence the properties of the final sol-gel material. Therefore, all CPO sol-gel beads that are compared within one table or graph were prepared on the same day with the same reagent batches in parallel.


### 3.3. Modification of Sol-Gel Procedure with respect to Methanol Release

Next, we tested whether minor modifications in the sol-gel procedure that influence the retention of methanol released from the sol-gel precursor TMOS would result in any significant changes. On the same day, three different sets of CPO sol-gel beads all containing 4μg/mL CPO were prepared in triplicate. The first set was prepared with a procedure that facilitates methanol release by using an open vessel while sonicating the sol solution and performing daily buffer exchanges during the first week of sol-gel maturation. The second and third sets were prepared using a closed sonication vessel and no buffer exchange was performed for the third set. Despite these modifications, all three sets prepared on the same day yielded virtually identical specific activity values of 167±18 IU/mg, 164±10 IU/mg, and 178±21 IU/mg, respectively. A more vigorous procedure presented by Ferrer et al. [31] involves rotavaporization of the sol solution prior to addition of the buffered enzyme solution. In our laboratory, this method was not successful as sol solidification started to set in too rapidly to achieve consistent gelation.Overall, the catalytic performance of the best set of sol-gel beads was only12.5±1.5% in comparison to the reference assay with free CPO in solution. Possible reasons for the decline in catalytic performance upon entrapment include (1) loss of enzyme due to leakage from the sol-gel matrix, (2) damage to the enzyme caused by the entrapment procedure, or (3) hindered substrate or product diffusion within the sol-gel nanostructure. As mentioned above, enzyme leakage did occur, but it can only account for a small loss of approximately 10%. In the experiments described below, we tested for the two remaining possible reasons for the decline in catalytic performance of the sol-gel entrapped CPO and also examined the effect of methanol on CPO.


### 3.4. CD and UV/VIS Spectroscopy with Sol-Gel Entrapped CPO

To monitor possible enzyme damage, we exploited the fact that sol-gels prepared from TMOS are transparent. Circular dichroism and visible absorbance spectra of free CPO in solution and CPO in sol-gel entrapped form are shown in Figures4 and 5, respectively. The spectra of free CPO and sol-gel entrapped CPO are virtually identical to each other. Minor changes in intensity of the spectroscopic signals are most likely due to scattering effects from the sol-gel surface or the shrinkage of the sol-gel material during the maturation process. Shrinkage slightly raises the concentration of the sample but also decreases the spectroscopic path length. The CD spectra are typical for a protein with high alpha helical content. This finding agrees with the X-ray protein structure of CPO [32] and previously determined CD data [15, 33].Figure 4
CD spectra of sol-gel entrapped CPO (black squares) and free CPO (white squares). The CPO concentration was 7μM in the silica sol-gel sheet of ~1 mm thickness. The solution sample containing 7 μM CPO in 2 mM sodium phosphate buffer, pH 7.0, was measured in a cuvette of 1 mm path length.Figure 5
Absorbance spectra of sol-gel entrapped CPO (solid line) and free CPO (dashed line). The silica sol-gel sheet was ~1 mm thick and contained 1.6 mg/mL CPO. The sheet was placed on the wall of a 10 mm wide plastic cuvette and immersed in 50 mM potassium phosphate buffer, pH 6.0. The solution reference sample was prepared from the same CPO stock via dilution to 0.16 mg/mL (4μM) with 50 mM potassium phosphate buffer, pH 6.0 in a 10 mm thick plastic cuvette.The visible absorbance spectra shown in Figure5 are typical for the active form of CPO with a five-coordinate iron center in the heme chromophore [34]. As the pH value increases above pH 7.0, CPO is inactivated, the iron center in the heme group becomes six-coordinate, and the Soret band shifts to a longer wavelength [34]. This spectroscopic transition can also be observed in CPO sol-gel sheets despite the entrapment of the enzyme inside the silica matrix. Figure 6 displays the absorption spectra of CPO sol-gel sheets immersed in 0.5 M phosphate buffers at pH values of 6.0, 8.0, and 10.0. In comparison to solution spectra, more alkaline conditions are necessary to achieve the Soret peak characteristic for sixfold coordinated heme iron centers. It is conceivable that the silanol groups in the sol-gel impose an additional buffering effect. According to Dunn and Zink [35], the pH can be up to one pH unit lower inside a sol-gel pore than in the surrounding aqueous buffer.Figure 6
Absorbance spectra of sol-gel entrapped CPO immersed in 0.5 M potassium phosphate buffer with pH values of 6.0 (solid line), 8.0 (dashed line), and 10.0 (dotted line). The silica sol-gel sheets of 1 mm thickness loaded with 1.6 mg/mL CPO were placed on the wall of plastic cuvette with a path length of 10 mm.The low apparentkcat values for sol-gel entrapped CPO (see Table 1) indicate that a significant number of entrapped CPO molecules were unable to catalyze the peroxidation of pyrogallol in an effective manner. CD and visible absorption spectra, however, did not indicate any enzyme damage. It should be noted that both spectroscopic methods can only address specific features of the enzyme. These features are the overall secondary structure of the protein, the electronic configuration of the active site chromophore, and the ability to rearrange the coordination sphere of the heme iron center in response to an external change in pH value.


### 3.5. Influence of Methanol on the Spectroscopic Features and Activity of Free CPO

Methanol is released from the sol-gel precursor TMOS. If we assume complete hydrolysis of TMOS and no evaporation of methanol, the protein is exposed to a methanol concentration of 11% v/v as the buffered enzyme solution and the sol are mixed and pipetted onto the parafilm for gelation and drying. The transfer into storage buffer and the subsequent exchange of buffer drastically lower the methanol content during the maturation phase of the sol-gel. After the first buffer exchange, the methanol concentration is only 0.03% v/v.To study the influence of methanol on the spectroscopic features of CPO, the enzyme was incubated with 11% v/v methanol and UV/VIS and CD spectra were recorded. We did not detect a change in the spectroscopic features of the CD spectra after several hours of incubation (data not shown), but methanol exposure did have a small and immediate effect on UV/VIS absorption spectra (see Figure7). The absorption maximum of the Soret band shifted from 396 nm to 400 nm. Notably, this shift was reversible via dialysis. The methanol removal caused a 1.3-fold increase in sample volume. We adjusted the corresponding spectroscopic trace in Figure 7 for this dilution effect.Figure 7
Absorption spectra of 9μM CPO in 0.1 M citrate-0.2 M phosphate buffer, pH 4.2 (solid line), in 11% v/v methanol and 0.1 M citrate-0.2 M phosphate buffer, pH 4.2 (dashed line), dialyzed sample (grey solid line), and dialyzed sample adjusted for 1.3-fold volume increase (grey dotted line).In agreement with previous studies [36], we observed a drastic decline in CPO’s catalytic performance after incubation of CPO with organic solvents. Compared to an aqueous reference sample without methanol, only 57% residual activity was detected after incubation with 11% v/v methanol for 2 hours. After one day, the residual activity still remained at 57%. We further discovered that the detrimental effect of methanol was reversible. Up to 96% of the sample’s initial activity was recovered after dialysis. Sample dilution also resulted in the enzyme’s recovery. Decreasing the methanol content to 5% or 1% v/v via sample dilution resulted in 90% and 100% relative activity in comparison to identically diluted samples from the same CPO batch that were not exposed to methanol. Our finding that damage caused by methanol was reversible in solution has implications for other studies on the use of CPO in organic solvents or in biphasic solvent systems [36, 37]. Several CPO substrates which can be converted into products of industrial interest have high solubility in nonpolar organic solvents [8].Also, if any initial damage caused by methanol exposure was also reversible for sol-gel entrapped CPO, immediate reduction in methanol content via evaporation of methanol from the sol solution or daily buffer exchanges during the gel maturation phase would not critically alter the final performance of the CPO sol-gel beads. This might explain why the three different, but parallel, preparations outlined in Section3.3 resulted in virtually identical performance for the three different CPO sol-gel bead sets. On the other hand, manifestation of unrecoverable enzyme damage would explain the low apparent kcat values determined for sol-gel entrapped CPO (see Table 1). It is conceivable that recovery from damage caused by methanol exposure is less effective for CPO molecules entrapped within the silica sol-gel host compared to free CPO in solution.


### 3.6. Hindered Material Transport in CPO Sol-Gel Beads

After their first use, all CPO sol-gel beads adopted a persistent yellow coloration indicating the entrapment of the product purpurogallin. Attractive intermolecular forces and physical constraints both can delay the release of product molecules from the silica nanostructure. If product release becomes rate-limiting, the apparentkcat value decreases as observed in the enzyme kinetic analysis. Hindered substrate diffusion, however, was not supported by enzyme kinetic experiments as the KM and KI values for the main substrate pyrogallol were virtually identical for sol-gel entrapped and free CPO (see Table 1). We cannot explain why pyrogallol and purpurogallin would show different material transport properties inside the silica nanostructure. Both molecules have similar functional groups and purpurogallin (MW 220 g/mol) is only somewhat larger than pyrogallol (MW 116 g/mol). An alternative explanation would involve side-reactions forming alternate charged products or the trapping of reactive, colored intermediates.The alternative peroxidation substrate 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is particularly large (MW 515 g/mol) and the product of the peroxidation reaction carries a positive charge [24]. Deprotonated silanol groups on the sol-gel surface can provide negative countercharges. The catalytic performance of CPO sol-gel beads dropped from 12.6±3% with the substrate pyrogallol to 9 ± 0.4% with the substrate ABTS relative to the corresponding assay with free CPO in solution. The CPO sol-gel beads adopted the green color of the ABTS peroxidation product. Kadnikova and coworkers observed the formation of several side products for the peroxidation reaction of ABTS by horseradish peroxidase in a sol-gel matrix [38]. The peroxidation reaction with ABTS and possibly other substrates, including pyrogallol, might therefore be more complex than in solution.We further observed that preequilibration of CPO sol-gel beads with pyrogallol considerably improved catalytic performance. For example, preequilibration with 72 mM pyrogallol for 3 hours in comparison to using 35 mM pyrogallol without preequilibration increased the relative activity from 12.3 to 19.6%. The strategy to preequilibrate enzyme sol-gel materials with an excess of substrate before adding a cosubstrate was already successfully applied by Smith and coworkers [23] in their study on sol-gel encapsulated horseradish peroxidase. Based on the data presented in Figure 1(a), however, we would not expect an increase in catalytic performance for sol-gel entrapped CPO as the pyrogallol concentration is raised from 35 to 72 mM. In fact, substrate inhibition should slightly lower the activity of CPO. Adsorption of pyrogallol molecules to the sol-gel surface or physical entrapment could further enhance the local effective concentration of pyrogallol around the enzyme. One key difference between the experiment leading to Figure 1(a) and the preequilibration experiment is the timing of adding the cosubstrate H2O2. Manoj et al. [29] argue that substrate inhibition of CPO is not simply caused by blocking the active site of the enzyme with excess substrate molecules. Instead they propose more complex substrate inhibition mechanisms that involve secondary conversion of an already formed product or competition by transient intermediates leading to alternate products. Both substrate inhibition scenarios require the immediate presence of the cosubstrate H2O2.


### 3.7. Modification of Sol-Gel Procedure Using MTMS

To modify the surface of the sol-gel material, we incorporated MTMS at molar ratios of 5, 20, and 40% in the sol solution. The addition of MTMS will introduce nonpolar methyl groups rendering the surface of the silica nanostructure more hydrophobic [39]. The casting buffer had a pH value of 6.0 and the total CPO loading was 4 μg/mL. The addition of MTMS resulted in longer gelation times, for example, up to 240 minutes for a molar ratio of 40% MTMS. Unfortunately, the beads prepared with MTMS were more brittle and fragile than any of the other CPO sol-gel beads prepared in this study. The brittleness of the beads rendered their handling more challenging. Regardless of the amount of incorporated MTMS, the activity was approximately 14±1% compared to a solution reference assay. The cumulative leakage often exceeded 20%. We cannot rule out that the physical instability of the beads during and after a buffer exchange might have contributed to higher apparent leakage and higher apparent activity values. In contrast to other enzymes, notably lipase, which showed interfacial activation and performed better inside more hydrophobic nanostructures [40], the incorporation of MTMS into the CPO sol-gel material did not improve catalytic performance in a systematic manner.


### 3.8. Modification of Sol-Gel Procedure Using More Acidic Casting Buffers

The enzyme CPO is stable under acidic conditions [24]. We exploited this CPO specific property and prepared CPO sol-gel beads using casting buffers with pH values of 4.5, 5.5, and 6.5. All sample preparations were conducted in parallel with the same batches of CPO, TMOS, and buffer reagents. The gelation time increased with more acidic casting buffers, but the sol-gel beads remained easy to handle and transparent. The CPO loading was 4 μg/mL. All CPO sol-gel preparations were divided into two portions. One portion was used for porosimetry studies and the other portion was used for leakage and activity measurements (see Table 3). The properties of CPO sol-gel beads cast at pH 4.5 and 5.5 are virtually identical, but the CPO sol-gel beads cast at pH 6.5 show significantly lower values in all categories. This indicates a change in the matrix formation of the sol-gel as the casting pH drops to or below pH 5.5. Overall, the porosimetry data is positively correlated with catalytic performance and, unfortunately, leakage. All three porosimetric properties, including larger average pore size, BET surface area, and pore volume, indicate reduced steric hindrance for material transport inside the sol-gel nanostructure. As a consequence, catalytic performance increased. Smaller average pore sizes, on the other hand, can aid in the retention of CPO.Table 3
Properties of CPO sol-gel beads prepared with different casting buffers.
pH of casting buffer
4.5
5.5
6.5
BET surface area (m2/g)
710 ± 40∗
740 ± 60
470 ± 40
Total pore volume (cm3/g)
0.58 ± 0.02
0.60 ± 0.06
0.32 ± 0.04
Average pore diameter (nm)
3.3 ± 0.3
3.2 ± 0.1
2.7 ± 0.1
Activity (mIU)
251 ± 2
242  ±  29
161 ± 13
Specific activity (IU/mg)
157 ± 1
151 ± 18
100 ± 8
Activity compared to free CPO (%)#
18
17
11
Cumulative leakage (%)†
13
14
9
∗All data are presented as mean values ± one standard deviation of triplicate data sets. #The relative activity was based on a reference assay with free CPO in solution yielding 887 ± 31 IU/mg. †The cumulative leakage over ten days of maturation corresponds to the summation of the leakage data shown in Figure 8.Figure 8
The storage buffer of CPO sol-gel beads prepared with casting buffers at pH 4.5 (dark grey bars), 5.5 (grey bars), and 6.5 (light grey bars) was exchanged on a daily basis and monitored for CPO activity. All samples were prepared in triplicate with eight CPO sol-gel beads per sample tube. The bar height represents the mean value and the error bar ± one standard deviation.The dimensions of the protein CPO are 5.3 nm × 4.6 nm × 6.0 nm [19]. The average pore diameters of approximately 3 nm are only slightly smaller than the size of CPO. Nevertheless, CPO remained well entrapped after completion of the sol-gel maturation phase. Attractive electrostatic forces did not most likely aid in the retention of CPO as the storage buffer had a pH value of 4.2 which is close to the isoelectric point of CPO. The isoelectric point of CPO fromC. fumago was calculated to be approximately 4.0 [18, 21]. Isoelectric focusing experiments on CPO fromPseudomonas pyrrocinia yielded an isoelectric point of 4.1 [41]. For all buffer conditions employed in our study, the net charge on the surface of CPO is therefore either close to zero or negative.Our observation that more acidic casting buffers result in greater porosimetry of sol-gels agrees well with several previous studies [42, 43]. However, not all enzymes will respond well to the use of more acidic casting conditions. Notably, enzymes have different pH profiles and some enzymes are inactive under acidic conditions. Sol-gel entrapped cholinesterase, for example, showed better performance in silica nanostructures prepared at pH values of 7.0 and 8.0 and then 6.0 [44].


## 3.1. Peroxidation of Pyrogallol Catalyzed by Free and Sol-Gel Entrapped CPO

The catalytic activity of CPO was assessed by monitoring the peroxidation reaction of pyrogallol. To determine optimal reaction conditions, we varied the pH value of the assay buffer and the concentrations of pyrogallol and hydrogen peroxide. Our observations for free CPO agree well with previous studies [24, 29]. Acidic conditions, with pH values in the range of 3.5 to 4.5, and pyrogallol concentrations at approximately 35 mM resulted in optimum catalytic performance. Very high pyrogallol concentrations gave rise to substrate inactivation, but the enzyme was even more sensitive towards inactivation by the cosubstrate H2O2. The H2O2 concentration should not exceed 10 mM. The enzyme kinetic data obtained for sol-gel entrapped CPO displayed similar features albeit at much lower specific activity. Notably, sol-gel entrapment did not abolish the detrimental effect of H2O2. All data displayed in Figure 1 was thus fitted with a substrate inhibition model (see (1)).Figure 1
Peroxidation of pyrogallol catalyzed by free CPO (open squares; 0.575μg CPO in 3 mL total assay volume) and sol-gel entrapped CPO (grey circles; 0.575 μg CPO entrapped in three sol-gel beads placed in 3 mL total assay volume). The CPO sol-gel beads were prepared from TMOS with a pH 6.0 casting buffer and matured for one week with a buffer exchange on every other day. The activity assays were performed in 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2 with constant concentrations of either 2.7 mM H2O2 (a) or 35 mM pyrogallol (b). All data were measured in triplicate and are displayed as mean values ± one standard deviation. The parameters of the curve fits are summarized in Table 1.
(a)
(b)Table 1
Enzyme kinetic parameters for the CPO catalyzed peroxidation of pyrogallol∗.
K
M (mM)
K
I (mM)
v
max
⁡ (IU/mg)
k
cat (1/sec)
Pyrogallol
Free CPO (R2 = 0.983)
11 ± 1.0
160  ± 20
2000 ± 80
1400 ± 60
Sol-gel entrapped CPO (R2 = 0.847)
9.3 ± 2.6
190 ± 74
100 ± 12
70 ± 8.4
H2O2
Free CPO (R2 = 0.921)
17 ± 6.2
6.4 ± 2.2
9100 ± 2500
6370 ± 1750
Sol-gel entrapped CPO (R2 = 0.657)
27 ± 39
6.1 ± 8.6
680 ± 830
480 ± 580
∗Enzyme kinetic parameters were obtained by fitting the data shown in Figure 1(a) (pyrogallol) and Figure 1(b) (H2O2) to a substrate inhibition model (see (1)). All parameters are presented as value of the fit ± one standard error of the fit.The enzyme kinetic parameters for the peroxidation of pyrogallol catalyzed by free and sol-gel entrapped CPO are summarized in Table1. Significantly smaller vmax and thus kcat values were obtained for sol-gel entrapped CPO compared to free CPO. The parameters Km and KI did not change significantly in response to sol-gel entrapment for either substrate. The poor quality of the fit shown in Figure 1(b) for the data on sol-gel entrapped CPO might be caused by large data point variations and the limitations of the substrate inhibition model. Manoj et al. [29] demonstrated that the substrate inhibition model used here (see (1)) can yield acceptable fits for individual substrate variations, but global fit parameters were shown to be unattainable [29]. For example, the KM and KI values of one substrate depended on the concentration of the other cosubstrate. All kinetic parameters determined here are thus only apparent values and the parameters for sol-gel entrapped CPO are further influenced by additional factors. For example, kcat was calculated based on the assumption that all entrapped CPO molecules are able to participate in the catalytic process. This will not be the case for CPO molecules that are entrapped in closed silica pores or are otherwise permanently shielded and for CPO molecules with compromised functionality due to enzyme damage. The decrease in kcat (and also vmax) might thus signify an apparent decrease in accessible and/or active CPO molecules upon sol-gel entrapment. Notably, hindered product release can also cause a decrease in kcat. The kcat value is either determined by the rate of catalytic turnover or the rate of product release, depending on which of the two steps is slower and thus rate-limiting. The silica nanostructure does not seem to impose any significant diffusional constraints on the molecules pyrogallol and possibly H2O2. If these substrate molecules were encountering substantial diffusional barriers, an increase in their apparent KM and KI values would be expected.


## 3.2. Entrapment and Catalytic Performance of CPO in Sol-Gel Beads

To further assess the entrapment and catalytic performance of the immobilized enzyme, different amounts of CPO were immobilized inside sol-gel beads corresponding to final concentrations of 40μg/mL, 8 μg/mL, and 4 μg/mL per total sol-gel solution. The storage buffer was exchanged to remove methanol originating from the hydrolysis and condensation steps of the sol-gel formation. CPO activity was measured in these exchanged buffer samples and compared to a reference sample with free CPO in solution to quantify enzyme leakage (see Figure 2). Higher enzyme loading resulted in more enzyme leakage. As the sol-gel material matured and more connections formed within the silica mesh, enzyme leakage declined.Figure 2
Three sets of CPO sol-gel beads with CPO concentrations of 40μg/mL (dark grey bars), 8 μg/mL (grey bars), and 4 μg/mL (light grey bars) were aged for one week. The storage buffer was replaced in the time intervals indicated on the graph and tested for CPO leakage. Each set was replicated three times and contained three beads per sample tube. The specific activity of the reference sample with free CPO in solution was 1436±39 IU/mg. The column heights represent the mean value and the error bars represent ± one standard deviation.Table2 contains the results of the activity measurements with the CPO sol-gel beads and summarizes the cumulative leakage over the first six days. The activity measurements were performed with CPO sol-gel beads that were seven days old. Each assay comprised 0.1 M citric acid-0.2 M dibasic sodium phosphate buffer, pH 4.2, 2.7 mM H2O2, and 35 mM pyrogallol. The CPO sol-gel beads with the highest CPO loading of 40 μg CPO per mL sol-gel yielded the highest absolute activity values of 249±33 mIU. A comparison among specific activity values reported per mg of initially loaded CPO, however, clearly showed that the CPO sol-gel beads with a lower enzyme content of 8 or 4 μg/mL performed better. At a higher loading of CPO, more CPO molecules might be obstructed by either other enzyme molecules or the silica nanostructure. Also, some of these CPO molecules might be entrapped in closed pores.Table 2
Catalytic performance of sol-gel beads loaded with different CPO amounts.
CPO loading of sol-gel beads (µg/mL)
40
8
4
Activity (mIU)∗
249 ± 33
138 ± 18
76 ± 4
Specific activity (IU/mg)∗
41 ± 6
115 ± 15
127 ± 6
Relative activity compared to free CPO (%)#
2.9
8.0
8.80
Cumulative leakage for six days (%)†
15
12
7
∗Absolute activity values in mIU (×10−3 International Units) and specific activity values per mg initially loaded CPO are presented as mean values ± one standard deviation. All sample sets were prepared in triplicate. #The relative activity was based on a reference assay with free CPO in solution. The reference value was 1436 ± 39 IU/mg. †The cumulative leakage over six days of maturation corresponds to the summation of the leakage data shown in Figure 2.Another set of three tubes each containing three beads loaded with 4μg/mL CPO was aged for one week and tested for reusability (see Figure 3). The CPO sol-gel beads can be reused up to three times in a convenient manner by replacing the liquid phase composed of buffer and product molecules with new buffer and substrate. However, a further decline in catalytic performance was apparent. We also noticed that all CPO sol-gel beads adopted the yellow-orange color of the product purpurogallin after the first use. Gentle washing with the storage buffer diminished the coloration only slightly. Thus, clogging of the sol-gel nanostructure with product molecules was one factor that hampered the reusability of the CPO sol-gel beads. Furthermore, the peroxidation reactions catalyzed by CPO are prone to substrate inhibition (see Figure 1). Trapped pyrogallol and H2O2 molecules could therefore interfere with effective reuse. Jung and Hartmann demonstrated thatin situ generation of hydrogen peroxide via coimmobilization of glucose oxidase can improve the reusability of cross-linked CPO molecules entrapped in mesoporous molecular sieves [30].Figure 3
Reusability test for CPO sol-gel beads loaded with 4μg/mL CPO. Three sets, each with three beads per tube, were used in this test. The column heights represent the mean specific activity value and the error bars represent ± one standard deviation. The first measurement was taken after the beads were aged for one week with daily buffer exchanges. The number of days that passed before the next reuse is listed on the x-axis.The specific activity of three independently prepared sets of sol-gel beads all containing ~4μg/mL CPO was different: 70±15 IU/mg (Figure 1), 127±6 IU/mg (Table 2), and 171±14 IU/mg (Figure 3). The CPO sol-gel materials used for generating the data presented in Table 2 and Figure 3 were prepared from the same CPO vial with a free CPO reference value of 1436±39 IU/mg. The reference value for the first CPO vial was only 1280±80 IU/mg. However, many other experimental parameters, such as the exact sol-gel composition, the quality of all starting materials, humidity, temperature, and duration of sol-gel drying phase, can also influence the properties of the final sol-gel material. Therefore, all CPO sol-gel beads that are compared within one table or graph were prepared on the same day with the same reagent batches in parallel.


## 3.3. Modification of Sol-Gel Procedure with respect to Methanol Release

Next, we tested whether minor modifications in the sol-gel procedure that influence the retention of methanol released from the sol-gel precursor TMOS would result in any significant changes. On the same day, three different sets of CPO sol-gel beads all containing 4μg/mL CPO were prepared in triplicate. The first set was prepared with a procedure that facilitates methanol release by using an open vessel while sonicating the sol solution and performing daily buffer exchanges during the first week of sol-gel maturation. The second and third sets were prepared using a closed sonication vessel and no buffer exchange was performed for the third set. Despite these modifications, all three sets prepared on the same day yielded virtually identical specific activity values of 167±18 IU/mg, 164±10 IU/mg, and 178±21 IU/mg, respectively. A more vigorous procedure presented by Ferrer et al. [31] involves rotavaporization of the sol solution prior to addition of the buffered enzyme solution. In our laboratory, this method was not successful as sol solidification started to set in too rapidly to achieve consistent gelation.Overall, the catalytic performance of the best set of sol-gel beads was only12.5±1.5% in comparison to the reference assay with free CPO in solution. Possible reasons for the decline in catalytic performance upon entrapment include (1) loss of enzyme due to leakage from the sol-gel matrix, (2) damage to the enzyme caused by the entrapment procedure, or (3) hindered substrate or product diffusion within the sol-gel nanostructure. As mentioned above, enzyme leakage did occur, but it can only account for a small loss of approximately 10%. In the experiments described below, we tested for the two remaining possible reasons for the decline in catalytic performance of the sol-gel entrapped CPO and also examined the effect of methanol on CPO.


## 3.4. CD and UV/VIS Spectroscopy with Sol-Gel Entrapped CPO

To monitor possible enzyme damage, we exploited the fact that sol-gels prepared from TMOS are transparent. Circular dichroism and visible absorbance spectra of free CPO in solution and CPO in sol-gel entrapped form are shown in Figures4 and 5, respectively. The spectra of free CPO and sol-gel entrapped CPO are virtually identical to each other. Minor changes in intensity of the spectroscopic signals are most likely due to scattering effects from the sol-gel surface or the shrinkage of the sol-gel material during the maturation process. Shrinkage slightly raises the concentration of the sample but also decreases the spectroscopic path length. The CD spectra are typical for a protein with high alpha helical content. This finding agrees with the X-ray protein structure of CPO [32] and previously determined CD data [15, 33].Figure 4
CD spectra of sol-gel entrapped CPO (black squares) and free CPO (white squares). The CPO concentration was 7μM in the silica sol-gel sheet of ~1 mm thickness. The solution sample containing 7 μM CPO in 2 mM sodium phosphate buffer, pH 7.0, was measured in a cuvette of 1 mm path length.Figure 5
Absorbance spectra of sol-gel entrapped CPO (solid line) and free CPO (dashed line). The silica sol-gel sheet was ~1 mm thick and contained 1.6 mg/mL CPO. The sheet was placed on the wall of a 10 mm wide plastic cuvette and immersed in 50 mM potassium phosphate buffer, pH 6.0. The solution reference sample was prepared from the same CPO stock via dilution to 0.16 mg/mL (4μM) with 50 mM potassium phosphate buffer, pH 6.0 in a 10 mm thick plastic cuvette.The visible absorbance spectra shown in Figure5 are typical for the active form of CPO with a five-coordinate iron center in the heme chromophore [34]. As the pH value increases above pH 7.0, CPO is inactivated, the iron center in the heme group becomes six-coordinate, and the Soret band shifts to a longer wavelength [34]. This spectroscopic transition can also be observed in CPO sol-gel sheets despite the entrapment of the enzyme inside the silica matrix. Figure 6 displays the absorption spectra of CPO sol-gel sheets immersed in 0.5 M phosphate buffers at pH values of 6.0, 8.0, and 10.0. In comparison to solution spectra, more alkaline conditions are necessary to achieve the Soret peak characteristic for sixfold coordinated heme iron centers. It is conceivable that the silanol groups in the sol-gel impose an additional buffering effect. According to Dunn and Zink [35], the pH can be up to one pH unit lower inside a sol-gel pore than in the surrounding aqueous buffer.Figure 6
Absorbance spectra of sol-gel entrapped CPO immersed in 0.5 M potassium phosphate buffer with pH values of 6.0 (solid line), 8.0 (dashed line), and 10.0 (dotted line). The silica sol-gel sheets of 1 mm thickness loaded with 1.6 mg/mL CPO were placed on the wall of plastic cuvette with a path length of 10 mm.The low apparentkcat values for sol-gel entrapped CPO (see Table 1) indicate that a significant number of entrapped CPO molecules were unable to catalyze the peroxidation of pyrogallol in an effective manner. CD and visible absorption spectra, however, did not indicate any enzyme damage. It should be noted that both spectroscopic methods can only address specific features of the enzyme. These features are the overall secondary structure of the protein, the electronic configuration of the active site chromophore, and the ability to rearrange the coordination sphere of the heme iron center in response to an external change in pH value.


## 3.5. Influence of Methanol on the Spectroscopic Features and Activity of Free CPO

Methanol is released from the sol-gel precursor TMOS. If we assume complete hydrolysis of TMOS and no evaporation of methanol, the protein is exposed to a methanol concentration of 11% v/v as the buffered enzyme solution and the sol are mixed and pipetted onto the parafilm for gelation and drying. The transfer into storage buffer and the subsequent exchange of buffer drastically lower the methanol content during the maturation phase of the sol-gel. After the first buffer exchange, the methanol concentration is only 0.03% v/v.To study the influence of methanol on the spectroscopic features of CPO, the enzyme was incubated with 11% v/v methanol and UV/VIS and CD spectra were recorded. We did not detect a change in the spectroscopic features of the CD spectra after several hours of incubation (data not shown), but methanol exposure did have a small and immediate effect on UV/VIS absorption spectra (see Figure7). The absorption maximum of the Soret band shifted from 396 nm to 400 nm. Notably, this shift was reversible via dialysis. The methanol removal caused a 1.3-fold increase in sample volume. We adjusted the corresponding spectroscopic trace in Figure 7 for this dilution effect.Figure 7
Absorption spectra of 9μM CPO in 0.1 M citrate-0.2 M phosphate buffer, pH 4.2 (solid line), in 11% v/v methanol and 0.1 M citrate-0.2 M phosphate buffer, pH 4.2 (dashed line), dialyzed sample (grey solid line), and dialyzed sample adjusted for 1.3-fold volume increase (grey dotted line).In agreement with previous studies [36], we observed a drastic decline in CPO’s catalytic performance after incubation of CPO with organic solvents. Compared to an aqueous reference sample without methanol, only 57% residual activity was detected after incubation with 11% v/v methanol for 2 hours. After one day, the residual activity still remained at 57%. We further discovered that the detrimental effect of methanol was reversible. Up to 96% of the sample’s initial activity was recovered after dialysis. Sample dilution also resulted in the enzyme’s recovery. Decreasing the methanol content to 5% or 1% v/v via sample dilution resulted in 90% and 100% relative activity in comparison to identically diluted samples from the same CPO batch that were not exposed to methanol. Our finding that damage caused by methanol was reversible in solution has implications for other studies on the use of CPO in organic solvents or in biphasic solvent systems [36, 37]. Several CPO substrates which can be converted into products of industrial interest have high solubility in nonpolar organic solvents [8].Also, if any initial damage caused by methanol exposure was also reversible for sol-gel entrapped CPO, immediate reduction in methanol content via evaporation of methanol from the sol solution or daily buffer exchanges during the gel maturation phase would not critically alter the final performance of the CPO sol-gel beads. This might explain why the three different, but parallel, preparations outlined in Section3.3 resulted in virtually identical performance for the three different CPO sol-gel bead sets. On the other hand, manifestation of unrecoverable enzyme damage would explain the low apparent kcat values determined for sol-gel entrapped CPO (see Table 1). It is conceivable that recovery from damage caused by methanol exposure is less effective for CPO molecules entrapped within the silica sol-gel host compared to free CPO in solution.


## 3.6. Hindered Material Transport in CPO Sol-Gel Beads

After their first use, all CPO sol-gel beads adopted a persistent yellow coloration indicating the entrapment of the product purpurogallin. Attractive intermolecular forces and physical constraints both can delay the release of product molecules from the silica nanostructure. If product release becomes rate-limiting, the apparentkcat value decreases as observed in the enzyme kinetic analysis. Hindered substrate diffusion, however, was not supported by enzyme kinetic experiments as the KM and KI values for the main substrate pyrogallol were virtually identical for sol-gel entrapped and free CPO (see Table 1). We cannot explain why pyrogallol and purpurogallin would show different material transport properties inside the silica nanostructure. Both molecules have similar functional groups and purpurogallin (MW 220 g/mol) is only somewhat larger than pyrogallol (MW 116 g/mol). An alternative explanation would involve side-reactions forming alternate charged products or the trapping of reactive, colored intermediates.The alternative peroxidation substrate 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is particularly large (MW 515 g/mol) and the product of the peroxidation reaction carries a positive charge [24]. Deprotonated silanol groups on the sol-gel surface can provide negative countercharges. The catalytic performance of CPO sol-gel beads dropped from 12.6±3% with the substrate pyrogallol to 9 ± 0.4% with the substrate ABTS relative to the corresponding assay with free CPO in solution. The CPO sol-gel beads adopted the green color of the ABTS peroxidation product. Kadnikova and coworkers observed the formation of several side products for the peroxidation reaction of ABTS by horseradish peroxidase in a sol-gel matrix [38]. The peroxidation reaction with ABTS and possibly other substrates, including pyrogallol, might therefore be more complex than in solution.We further observed that preequilibration of CPO sol-gel beads with pyrogallol considerably improved catalytic performance. For example, preequilibration with 72 mM pyrogallol for 3 hours in comparison to using 35 mM pyrogallol without preequilibration increased the relative activity from 12.3 to 19.6%. The strategy to preequilibrate enzyme sol-gel materials with an excess of substrate before adding a cosubstrate was already successfully applied by Smith and coworkers [23] in their study on sol-gel encapsulated horseradish peroxidase. Based on the data presented in Figure 1(a), however, we would not expect an increase in catalytic performance for sol-gel entrapped CPO as the pyrogallol concentration is raised from 35 to 72 mM. In fact, substrate inhibition should slightly lower the activity of CPO. Adsorption of pyrogallol molecules to the sol-gel surface or physical entrapment could further enhance the local effective concentration of pyrogallol around the enzyme. One key difference between the experiment leading to Figure 1(a) and the preequilibration experiment is the timing of adding the cosubstrate H2O2. Manoj et al. [29] argue that substrate inhibition of CPO is not simply caused by blocking the active site of the enzyme with excess substrate molecules. Instead they propose more complex substrate inhibition mechanisms that involve secondary conversion of an already formed product or competition by transient intermediates leading to alternate products. Both substrate inhibition scenarios require the immediate presence of the cosubstrate H2O2.


## 3.7. Modification of Sol-Gel Procedure Using MTMS

To modify the surface of the sol-gel material, we incorporated MTMS at molar ratios of 5, 20, and 40% in the sol solution. The addition of MTMS will introduce nonpolar methyl groups rendering the surface of the silica nanostructure more hydrophobic [39]. The casting buffer had a pH value of 6.0 and the total CPO loading was 4 μg/mL. The addition of MTMS resulted in longer gelation times, for example, up to 240 minutes for a molar ratio of 40% MTMS. Unfortunately, the beads prepared with MTMS were more brittle and fragile than any of the other CPO sol-gel beads prepared in this study. The brittleness of the beads rendered their handling more challenging. Regardless of the amount of incorporated MTMS, the activity was approximately 14±1% compared to a solution reference assay. The cumulative leakage often exceeded 20%. We cannot rule out that the physical instability of the beads during and after a buffer exchange might have contributed to higher apparent leakage and higher apparent activity values. In contrast to other enzymes, notably lipase, which showed interfacial activation and performed better inside more hydrophobic nanostructures [40], the incorporation of MTMS into the CPO sol-gel material did not improve catalytic performance in a systematic manner.


## 3.8. Modification of Sol-Gel Procedure Using More Acidic Casting Buffers

The enzyme CPO is stable under acidic conditions [24]. We exploited this CPO specific property and prepared CPO sol-gel beads using casting buffers with pH values of 4.5, 5.5, and 6.5. All sample preparations were conducted in parallel with the same batches of CPO, TMOS, and buffer reagents. The gelation time increased with more acidic casting buffers, but the sol-gel beads remained easy to handle and transparent. The CPO loading was 4 μg/mL. All CPO sol-gel preparations were divided into two portions. One portion was used for porosimetry studies and the other portion was used for leakage and activity measurements (see Table 3). The properties of CPO sol-gel beads cast at pH 4.5 and 5.5 are virtually identical, but the CPO sol-gel beads cast at pH 6.5 show significantly lower values in all categories. This indicates a change in the matrix formation of the sol-gel as the casting pH drops to or below pH 5.5. Overall, the porosimetry data is positively correlated with catalytic performance and, unfortunately, leakage. All three porosimetric properties, including larger average pore size, BET surface area, and pore volume, indicate reduced steric hindrance for material transport inside the sol-gel nanostructure. As a consequence, catalytic performance increased. Smaller average pore sizes, on the other hand, can aid in the retention of CPO.Table 3
Properties of CPO sol-gel beads prepared with different casting buffers.
pH of casting buffer
4.5
5.5
6.5
BET surface area (m2/g)
710 ± 40∗
740 ± 60
470 ± 40
Total pore volume (cm3/g)
0.58 ± 0.02
0.60 ± 0.06
0.32 ± 0.04
Average pore diameter (nm)
3.3 ± 0.3
3.2 ± 0.1
2.7 ± 0.1
Activity (mIU)
251 ± 2
242  ±  29
161 ± 13
Specific activity (IU/mg)
157 ± 1
151 ± 18
100 ± 8
Activity compared to free CPO (%)#
18
17
11
Cumulative leakage (%)†
13
14
9
∗All data are presented as mean values ± one standard deviation of triplicate data sets. #The relative activity was based on a reference assay with free CPO in solution yielding 887 ± 31 IU/mg. †The cumulative leakage over ten days of maturation corresponds to the summation of the leakage data shown in Figure 8.Figure 8
The storage buffer of CPO sol-gel beads prepared with casting buffers at pH 4.5 (dark grey bars), 5.5 (grey bars), and 6.5 (light grey bars) was exchanged on a daily basis and monitored for CPO activity. All samples were prepared in triplicate with eight CPO sol-gel beads per sample tube. The bar height represents the mean value and the error bar ± one standard deviation.The dimensions of the protein CPO are 5.3 nm × 4.6 nm × 6.0 nm [19]. The average pore diameters of approximately 3 nm are only slightly smaller than the size of CPO. Nevertheless, CPO remained well entrapped after completion of the sol-gel maturation phase. Attractive electrostatic forces did not most likely aid in the retention of CPO as the storage buffer had a pH value of 4.2 which is close to the isoelectric point of CPO. The isoelectric point of CPO fromC. fumago was calculated to be approximately 4.0 [18, 21]. Isoelectric focusing experiments on CPO fromPseudomonas pyrrocinia yielded an isoelectric point of 4.1 [41]. For all buffer conditions employed in our study, the net charge on the surface of CPO is therefore either close to zero or negative.Our observation that more acidic casting buffers result in greater porosimetry of sol-gels agrees well with several previous studies [42, 43]. However, not all enzymes will respond well to the use of more acidic casting conditions. Notably, enzymes have different pH profiles and some enzymes are inactive under acidic conditions. Sol-gel entrapped cholinesterase, for example, showed better performance in silica nanostructures prepared at pH values of 7.0 and 8.0 and then 6.0 [44].


## 4. Conclusion

The enzyme CPO was successfully entrapped inside a silica nanostructure prepared from the precursor TMOS with or without addition of the hydrophobic modifier MTMS. Since CPO is stabile in acidic buffers, we further modified the sol-gel procedure by using casting buffers with pH values of 4.5, 5.5, 6.0, and 6.5. The catalytic performance of optimized CPO sol-gel beads approached 18% relative to free CPO in solution as assessed via the pyrogallol peroxidation assay. A combination of factors, such as enzyme leakage from the sol-gel host, insufficient recovery from inactivation caused by initial methanol exposure, hindered product release, or alternate reaction pathways, are most likely responsible for the decline in catalytic performance of CPO after sol-gel entrapment. The use of more acidic casting buffers in the sol-gel procedure provided the most leverage for optimization by yielding more porous silica nanostructures. Overall, our findings are of importance for the optimization of other sol-gel materials devised for applications in biosensing or biocatalysis or designed for the controlled release of bioactive compounds.



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*Source: 632076-2015-10-01.xml*