# Investigating Methylene Blue Adsorption and Photocatalytic Activity of ZnO/CNC Nanohybrids

Authors: Vu Nang An; Tran T. T. Van; Ha T. C. Nhan; Le Van Hieu
Journal: Journal of Nanomaterials
 (2020)

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## Abstract

Nanohybrids of zinc oxide/cellulose nanocrystals (ZnO/CNCs) were successfully prepared by using a low cost and green method for adsorption and photocatalytic degradation of methylene blue (MB). CNCs have been derived through the hydrolysis reaction by citric/hydrochloric acid from the pure cellulose isolated from VietnameseNypa fruticans trunk. The influence of the Zn2+ ion concentration on the morphology, microstructure, and thermal properties as well as the photocatalytic activity of the ZnO/CNC nanohybrids was investigated in detail. Analyses of FTIR spectra, XRD, and SEM indicated that the ZnO nanocrystals with the size of 50 nm formed and loaded on the surface of CNC. Based on the DRS spectra and the nitrogen adsorption–desorption isotherms (BET) analysis, the absorption of ultraviolet light with a strong absorption band around 400 nm was found for all the ZnO/CNC nanohybrids, and the values of specific surface areas (SBET) of materials can be controlled by changing the concentration ratio of Zn2+ ion and CNC. The TGA analysis demonstrated that the ZnO loading samples (ZnO/CNC) had the thermal degradation onset temperature higher than that of neat CNC. The effect of MB removal showed the results which were contributed not only by the adsorption ability of CNC but also by the photocatalytic activity of ZnO. The photocatalytic efficiency significantly depended on the content of ZnO loading. The maximum degradation of MB was about 95% in 150 min for the ZnO/CNC-1.0 sample in which the concentration ratio of zinc-precursor Zn(NO3)2·6H2O and CNC was 1.0.


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## Body

## 1. Introduction

Cellulose as one of the most abundant natural biopolymers has been widely used as a reinforcing material for fiber–thermoplastic composite materials [1–5]. Cellulose nanocrystals (CNCs) prepared by acid hydrolysis of natural cellulose are typically formed of a rigid rod-shaped monocrystalline domain at different nanosizes of 1–100 nm (in diameter), depending on the biomass source [6]. CNCs have been using as nanofillers in polymer composites, in food and cosmetics, etc. [7, 8]. CNCs have many advantage aspects such as excellent mechanical properties, high aspect ratio, nanoscale dimension, low cost, availability, and renewability [9]. However, monofunctional CNC can be only applied in various biomedical and photocatalytic fields since many researchers are trying to make its more functionalization such as chemical modification and doping with other nanomaterials.ZnO nanoparticles (ZnO NPs) were reported to have the notable optoelectronic properties, high catalytic activity for chemical and biological species, and strong antimicrobial properties for wide range of pathogens [10]. Having the band gap of ZnO of 3.3 eV similar to that of TiO2 (3.2 eV for anatase), the ZnO shows the also remarkable optical and photocatalytical properties. Therefore, ZnO was also found in many applications in functional optical devices, ultraviolet photodetectors, gas sensors, solar cells, ultraviolet laser diodes, and ion insertion batteries [11–14] and also in medical and pharmaceutical industries for drug delivery and cosmetics manufacturing. In other hand, one of attracting recent development of ZnO application in waste water treatment is for the degradation of pollutants including organic dyes, effluents, and also for the removal of heavy metals during waste water purification [15–17]. However, ZnO nanoparticles tend to aggregate due to their large surface area and high surface energy. To overtake this problem and improve nanoscale dispersion, the fabrication of ZnO nanoparticles in using the nanofibrillar materials (such as cellulose and its derivatives) as a substrate has attracted attention for many potential applications in different areas. Although the research on cellulose-based nanocomposites is continuously growing, there are few studies published on the synthesis of ZnO NPs on the cellulose substrate. These studies reported the complicated preparation methods and take a longtime process of experiment, which are not efficient for industrial application. For example, Ul-Islam et al. [18] have used three time-experimental steps to prepare ZnO/CNC nanohybrids by using regenerated bacterial cellulose (RBC) templates. The obtained nanohybrids showed efficient antibacterial properties, but the degradation temperatures were increased slightly by 5–10°C compared with neat RBC. More recently, a two-step preparation method for ZnO/CNC nanohybrids was reported. The first step is the preparation of CNC using hydrochloric acid hydrolysis and then the next is the formation of ZnO nanoparticles on the surface of the CNC using a precipitation method. This result was shown the relatively weak interactions between the CNC and ZnO nanoparticles due to the weak electrostatic interactions between the hydroxyl groups on the surface of the CNC and the Zn2+ [19, 20]. The achieved ZnO/CNC nanohybrids exhibited stronger antibacterial activity than the CNC ions. In this work, a simple, green one-step synthesis technique was applied to fabricate ZnO/CNC hybrids at mild temperature of 80°C in using solely water solvent and especially Vietnamese biosource Nypa fruticans cellulose nanocrystals which were used as the biotemplate in order to improve the adsorption and photocatalytic efficiency of ZnO NPs. Moreover, the morphology, structure, and properties of the ZnO/CNC nanohybrids were investigated with different ratios. This method could be used as one of the approaches for the development of cheaper and more effective technologies for industrial wastewater purification.


## 2. Materials and Methods

Nypa Fruticans trunk (NFT) was collected from muddy areas along the riverside in Ben Tre, Southwest of Vietnam. Zinc nitrate (Zn(NO3)2.6H2O, 98%) and sodium hydroxide (NaOH, 96%) were purchased from Guangdong Guanghua Sci-Tech Co., Ltd., China. Formic acid (HCOOH, 90%), hydrogen peroxide (H2O2, 30%), and sulfuric acid (H2SO4, 98%) were procured from Xilong Scientific Co., Ltd., China.


### 2.1. Extraction of Cellulose from NFT

Cellulose was isolated from NFT as raw material by chemical treatment method [21]. First, the raw cellulose bulk of NFT was peeled off and cut into chunks around 30 cm in length and 1 cm2 of edge surface area. These chunks of NFT had been then laminated by a rolling machine before dried and splitted into fibers. Then, they were grinded into fine powder and stirred well in 1000 mL of distilled water at 100°C for 2 h to remove impurities and aqueous soluble organic substances. They were then cooled to room temperature and filtered. This process was conducted twice, and the final bulk celluloses were then dried in an air oven at 70°C for 8 h. Twenty grams of the pretreated biomass powder were dispersed into HCOOH 90% (1 : 10 w/v) solution at 100°C for 2 h by magnetic agitator in circulation system. This suspension was filtered, and the obtained residue was washed successively with pure HCOOH acid and warm distilled water to break the β-O-4 linkages of hemicellulose as well as dissolve the impurities remnant. To remove hemicellulose, after pretreatment with acid, samples were dissolved into PFA (a mixed solvent of formic acid, hydrogen peroxide, and distilled water with the ratio of 90 : 4 : 6% w/w, respectively) and stirred well at 80°C for 2 h. The suspension was then filtered and washed with formic acid (80%) and distilled water, respectively. Finally, the fiber was bleached with a combined solution of NaOH and H2O2 (1 : 1 w/w) in using a magnetic stirring circulation system at 80°C for 1 h, finally filtered and rinsed several times to get rid of lignin and hemicelluloses. This bleaching process was repeated two times until the fibers became white. It has been reported that apart from improving the physical appearance of fibers, bleaching procedure also helps in improvement of mechanical properties due to the better interfacial adhesion between fibers and matrix [22]. The cellulose was then obtained after filtration and three times washing with deionized water and allowed to air dry for 48 h.


### 2.2. Preparation of Zinc Oxide/Cellulose Nanocrystal (ZnO/CNC) Nanohybrids

ZnO/CNC nanohybrids were prepared by a one-pot synthesis route as reported in other paper [23] in which CNC with carboxyl groups was obtained to fabricate Zn(OH)2/CNC by simple precipitation through electrostatic interaction, and finally, ZnO/CNC nanohybrids were achieved by thermal treatment. In our experiment, the starting cellulose (8 g) was added to 400 mL mixed acid solution of 90% C6H8O7/10% HCl (400 mL, 3 M C6H8O7, and 6 M HCl). The mixture was heated at 80°C for 6 h under continuously mechanical stirring, and the suspension was rapidly cooled to room temperature. The functionalized CNC and recycled acid mixtures were then separated by filtration processes, and the CNC samples were washed 3 times with deionized water by centrifugation. The centrifuged samples were left overnight by drying in an oven at 80°C for several hours. This CNC powders were used directly to prepare ZnO/CNC nanohybrids by adding it to Zn(NO3)2.6H2O solutions (0.1 mol/L) with different weight ratios (Zn(NO3)2.6H2O/CNC powder) of 0.5, 1.0, and 2.0. The pH values of these three samples were adjusted to 7 by using NaOH solution (0.5 mol/L). Then, the mixture was heated to 80°C, and 0.1 M NaOH solution was added dropwise to precipitate Zn2+ completely with vigorous stirring. Zn(OH)2 nanoparticles were obtained by simple precipitation through electrostatic adsorption between carboxylates (COO-) of CNC and Zn2+. The mixture was then washed repeatedly by centrifugation (5,000 rpm for 20 min) with deionized water to remove remaining zinc species and by products. Finally, the samples were dried at 120°C for 1 h to transform Zn(OH)2 to ZnO. In the following manuscript, the three samples were labelled according to the weight ratio of Zn(NO3)2.6H2O to CNC powder as ZnO/CNC-0.5, ZnO/CNC-1.0, and ZnO/CNC-2.0. For reference, the as-produced CNC suspensions were also purified by deionized water to remove the remaining cellulose hydrolysis products.


### 2.3. Characterization

The structure morphologies of the CNC and ZnO/CNC nanohybrids were observed at room temperature by using the field-emission scanning electron microscopy (FE-SEM, S-4800; Hitachi, Japan) at acceleration voltage of 10.0 kV. Fourier-transform infrared (FT-IR) spectra of the samples were obtained by using an FT-IR spectrometer (Nicolet 6700; Thermo Fisher SCIENTIFIC, USA) using the KBr disc method. Disks containing 2 mg of sample were scanned in the wavenumber range of 400-4,000 cm-1. The crystalline phase and its crystallite size of synthesized CNC powders and ZnO/CNC nanohybrids were characterized by an X-ray powder diffractometer (XRD, D2 PHARSER; Bruker) with monochromatic Cu Kɑ radiation at λ=1.54056Å in the 2θ range of 10–80° at scan rate of 0.02°/min. The X-ray generator tension and current were 40 kV and 30 mA, respectively. The TGA experiment was conducted using a thermogravimetric analyzer (TA Instruments TGA Q500). The samples (15−20 mg) were heated at a rate of 10°C/min from room temperature to 700°C under a nitrogen flow rate of 30 mL·min−1. The specific surface area of our materials was measured via the nitrogen adsorption–desorption experiments on a NOVA 1000e analyzer (Quantachrome Instruments). The band gap energy (Eg) values of nanohybrids were determined from room temperature UV-visible diffuse reflectance spectra (DRS) using a Perkin-Elmer Lambda 850 Spectrophotometer equipped with a 15 cm diameter integrating sphere bearing the holder in the bottom horizontal position.


### 2.4. Adsorption and Photocatalytic Evaluation

The adsorption/photocatalytic efficiency of the samples was investigated by monitoring the removal of MB dye as a model compound. 40 mg of ZnO/CNC powders were dispersed into 50 mL of MB aqueous solution (10.0×10−5M). In order to determine adsorption equilibrium, the suspensions were stirred in dark for 2.5 h at 200 rpm. The saturation adsorption was obtained after 15 min. Continuously, the suspensions were irradiated by UVC lamp (15 W Osram Germicidal, λ=234nm) to activate the photocatalytic process. A certain amount of solution (~3 mL) was taken out at given time intervals (30 min), and a UV–vis spectrophotometer (model V-670, Jasco, Japan) was used to measure the absorbance of samples. The removal percentages (R) of MB, indicating adsorption ability, were calculated by using
(1)R=C0−CtC0×100%,where Co and Ct are the initial concentration and concentration of MB dye after the adsorption equilibrium without UVC irradiation, respectively. The degradation rate was calculated as follows:
(2)Degradationrate%=Co,−CtCt×100,where Co, represents the initial concentration of MB dye (after the absorption equilibrium) and Ct is the final concentration after illumination by UVC for t time.


## 2.1. Extraction of Cellulose from NFT

Cellulose was isolated from NFT as raw material by chemical treatment method [21]. First, the raw cellulose bulk of NFT was peeled off and cut into chunks around 30 cm in length and 1 cm2 of edge surface area. These chunks of NFT had been then laminated by a rolling machine before dried and splitted into fibers. Then, they were grinded into fine powder and stirred well in 1000 mL of distilled water at 100°C for 2 h to remove impurities and aqueous soluble organic substances. They were then cooled to room temperature and filtered. This process was conducted twice, and the final bulk celluloses were then dried in an air oven at 70°C for 8 h. Twenty grams of the pretreated biomass powder were dispersed into HCOOH 90% (1 : 10 w/v) solution at 100°C for 2 h by magnetic agitator in circulation system. This suspension was filtered, and the obtained residue was washed successively with pure HCOOH acid and warm distilled water to break the β-O-4 linkages of hemicellulose as well as dissolve the impurities remnant. To remove hemicellulose, after pretreatment with acid, samples were dissolved into PFA (a mixed solvent of formic acid, hydrogen peroxide, and distilled water with the ratio of 90 : 4 : 6% w/w, respectively) and stirred well at 80°C for 2 h. The suspension was then filtered and washed with formic acid (80%) and distilled water, respectively. Finally, the fiber was bleached with a combined solution of NaOH and H2O2 (1 : 1 w/w) in using a magnetic stirring circulation system at 80°C for 1 h, finally filtered and rinsed several times to get rid of lignin and hemicelluloses. This bleaching process was repeated two times until the fibers became white. It has been reported that apart from improving the physical appearance of fibers, bleaching procedure also helps in improvement of mechanical properties due to the better interfacial adhesion between fibers and matrix [22]. The cellulose was then obtained after filtration and three times washing with deionized water and allowed to air dry for 48 h.


## 2.2. Preparation of Zinc Oxide/Cellulose Nanocrystal (ZnO/CNC) Nanohybrids

ZnO/CNC nanohybrids were prepared by a one-pot synthesis route as reported in other paper [23] in which CNC with carboxyl groups was obtained to fabricate Zn(OH)2/CNC by simple precipitation through electrostatic interaction, and finally, ZnO/CNC nanohybrids were achieved by thermal treatment. In our experiment, the starting cellulose (8 g) was added to 400 mL mixed acid solution of 90% C6H8O7/10% HCl (400 mL, 3 M C6H8O7, and 6 M HCl). The mixture was heated at 80°C for 6 h under continuously mechanical stirring, and the suspension was rapidly cooled to room temperature. The functionalized CNC and recycled acid mixtures were then separated by filtration processes, and the CNC samples were washed 3 times with deionized water by centrifugation. The centrifuged samples were left overnight by drying in an oven at 80°C for several hours. This CNC powders were used directly to prepare ZnO/CNC nanohybrids by adding it to Zn(NO3)2.6H2O solutions (0.1 mol/L) with different weight ratios (Zn(NO3)2.6H2O/CNC powder) of 0.5, 1.0, and 2.0. The pH values of these three samples were adjusted to 7 by using NaOH solution (0.5 mol/L). Then, the mixture was heated to 80°C, and 0.1 M NaOH solution was added dropwise to precipitate Zn2+ completely with vigorous stirring. Zn(OH)2 nanoparticles were obtained by simple precipitation through electrostatic adsorption between carboxylates (COO-) of CNC and Zn2+. The mixture was then washed repeatedly by centrifugation (5,000 rpm for 20 min) with deionized water to remove remaining zinc species and by products. Finally, the samples were dried at 120°C for 1 h to transform Zn(OH)2 to ZnO. In the following manuscript, the three samples were labelled according to the weight ratio of Zn(NO3)2.6H2O to CNC powder as ZnO/CNC-0.5, ZnO/CNC-1.0, and ZnO/CNC-2.0. For reference, the as-produced CNC suspensions were also purified by deionized water to remove the remaining cellulose hydrolysis products.


## 2.3. Characterization

The structure morphologies of the CNC and ZnO/CNC nanohybrids were observed at room temperature by using the field-emission scanning electron microscopy (FE-SEM, S-4800; Hitachi, Japan) at acceleration voltage of 10.0 kV. Fourier-transform infrared (FT-IR) spectra of the samples were obtained by using an FT-IR spectrometer (Nicolet 6700; Thermo Fisher SCIENTIFIC, USA) using the KBr disc method. Disks containing 2 mg of sample were scanned in the wavenumber range of 400-4,000 cm-1. The crystalline phase and its crystallite size of synthesized CNC powders and ZnO/CNC nanohybrids were characterized by an X-ray powder diffractometer (XRD, D2 PHARSER; Bruker) with monochromatic Cu Kɑ radiation at λ=1.54056Å in the 2θ range of 10–80° at scan rate of 0.02°/min. The X-ray generator tension and current were 40 kV and 30 mA, respectively. The TGA experiment was conducted using a thermogravimetric analyzer (TA Instruments TGA Q500). The samples (15−20 mg) were heated at a rate of 10°C/min from room temperature to 700°C under a nitrogen flow rate of 30 mL·min−1. The specific surface area of our materials was measured via the nitrogen adsorption–desorption experiments on a NOVA 1000e analyzer (Quantachrome Instruments). The band gap energy (Eg) values of nanohybrids were determined from room temperature UV-visible diffuse reflectance spectra (DRS) using a Perkin-Elmer Lambda 850 Spectrophotometer equipped with a 15 cm diameter integrating sphere bearing the holder in the bottom horizontal position.


## 2.4. Adsorption and Photocatalytic Evaluation

The adsorption/photocatalytic efficiency of the samples was investigated by monitoring the removal of MB dye as a model compound. 40 mg of ZnO/CNC powders were dispersed into 50 mL of MB aqueous solution (10.0×10−5M). In order to determine adsorption equilibrium, the suspensions were stirred in dark for 2.5 h at 200 rpm. The saturation adsorption was obtained after 15 min. Continuously, the suspensions were irradiated by UVC lamp (15 W Osram Germicidal, λ=234nm) to activate the photocatalytic process. A certain amount of solution (~3 mL) was taken out at given time intervals (30 min), and a UV–vis spectrophotometer (model V-670, Jasco, Japan) was used to measure the absorbance of samples. The removal percentages (R) of MB, indicating adsorption ability, were calculated by using
(1)R=C0−CtC0×100%,where Co and Ct are the initial concentration and concentration of MB dye after the adsorption equilibrium without UVC irradiation, respectively. The degradation rate was calculated as follows:
(2)Degradationrate%=Co,−CtCt×100,where Co, represents the initial concentration of MB dye (after the absorption equilibrium) and Ct is the final concentration after illumination by UVC for t time.


## 3. Results and Discussion

### 3.1. Structure, Morphology, and Surface Area of CNC and ZnO/CNC Nanohybrids

#### 3.1.1. Structure

The XRD diffractograms of every NFT treatment stage and the CNC, ZnO, and ZnO/CNC-0.5 nanohybrids are shown in Figure1. The results present their CNC monoclinic structure. The (101), (002), and (040) lattice planes were observed at 2θ=16.15, 22.65, and 34.88°, respectively. These peaks correspond to polymorph of cellulose I (indexed to the database of ID number COD 4114994) [24]. XRD profiles of the cellulosic products obtained at each stage were analyzed to monitor the evolution of the percentage crystalline index (CrI) of nanocellulose. The physical and mechanical properties of cellulose are influenced by its crystalline structure. CrI is one important parameter of the crystal structure. The pretreatment of cellulose fibers increases their CrI meaning to the increasing of ratio of crystalline and amorphous region and surface roughness as well. This made it easy for the adhesion of ZnO onto the cellulose matrix. From XRD analysis, the CrI of cellulose and pretreated fibers was calculated in using the amorphous subtraction method described by Equation (3) [25]:
(3)CrI%=I002−IamI002×100,where I002 is the maximum diffraction intensity of the characteristic peak (002) at around 22–23° and Iam is the diffraction intensity attributed to amorphous cellulose, which is the valley between peaks (101) and (002). Treatment of the fibers increased their % CrI. The calculated % CrI were 57.1, 62.3, 78.9, and 84.5% for biomass obtained after acid, PFA, bleaching treatments, and the final acid hydrolysis, respectively. From these results, it can be deduced that each treatment step affected the crystallinity of the materials. The CrI was found to have increased by 26.9% after the removal of lignin and hemicellulose. The increasing % CrI indicates an improvement of the cellulose crystallinity.Figure 1
XRD patterns of different treatment stages by acid, PFA, bleaching, and the final acid hydrolysis for NFT (a) and CNC, ZnO, and ZnO/CNC-0.5 nanohybrids (b).
(a)
(b)The XRD patterns of ZnO with high intensity of the peaks revealed the single-phase crystalline nature of the synthesized ZnO nanostructures. The characteristic peaks correspond to the (100), (002), (101), (102), (110), (103), (200), (201), and (112) planes of ZnO were observed at2θ=31.89, 34.60, 36.39, 47.62, 56.67, 62.97, 66.47, 68.11, and 69.17°, respectively. This result is similar to those reported previously [16, 17] and are consistent with the database of ID number COD 1011258 with a wurtzite hexagonal crystallographic lattice of ZnO. The ZnO/CNC-0.5 XRD profile displays two-phase structures corresponding to cellulose (∗) and ZnO (#) as indicated in Figure 1(b). In addition, the XRD diagrams of ZnO/CNC-1.0 and ZnO/CNC-2.0 nanohybrids were similar to that of ZnO/CNC-0.5 in Figure 2, indicating the successful synthesis of this material by the facile one-step route. It can be deduced from these profiles that the presence of well crystalline ZnO has not altered the crystal structure of the cellulose matrix.Figure 2
XRD patterns of ZnO and ZnO/CNC nanohybrids.The average crystallite size (D) of cellulose, pure ZnO, and ZnO in the nanohybrids was calculated from XRD analysis using Equation (4): the Debye-Scherrer formula:
(4)D=0.9λβcosθ,where D is the crystallite size corresponding to the (hkl) plane, λ is the wavelength of copper Kα X-ray radiation (1.5406 Å), β is the observed angular width at half maximum intensity (FWHM), and θ is the Bragg diffraction angle measured in radians. In this work, the most intense diffraction lines ((100), (002), and (101)) were selected to calculate the average size of ZnO crystals. The lattice parameters a, b, and c, which are related to the interplanar distance (d) and Miller indices h, k, and l, were calculated for pure ZnO and ZnO in the nanohybrids using Equation (5) to determine the influence of cellulose nanocrystals
(5)1d2=43h2+hk+l2a2+l2c2.The calculated lattice parameters are reported in Table1. As observed in literature [22], compared to pure ZnO, the crystallite size of ZnO in the nanohybrids is much smaller, and there is a slight increase in the value of lattice parameters. According to Sharma et al. [26], the increase in the value of lattice parameters indicates the stretching of unit cells due to adsorption of polymer molecular chains on the surface of ZnO nanostructures.Table 1
Structural parameters of ZnO nanocrystals.
Sample
Crystallite size (nm)
Interplanar distance (d) (nm)
Lattice parameters
Lattice constantc/a
a=b (nm)
c (nm)
ZnO (pure)
32.8
0.2461
0.5323
0.2833
0.5323
ZnO/CNC-0.5
6.8
0.2489
0.5359
0.2872
0.5359
ZnO/CNC-1.0
9.1
0.2498
0.5369
0.2883
0.5369
ZnO/CNC-2.0
10.3
0.2489
0.5359
0.2872
0.5359The chemical structure of CNC and ZnO/CNC nanohybrids was analyzed using FTIR spectroscopy as shown in Figure3. Similar characteristic bands corresponding to CNC were observed for the ZnO/CNC nanohybrids at 3,375 and 1,380 cm-1, which can be related to stretching and bending vibrations of hydroxyl groups, respectively. The peaks at around 1,630 and 1,060 cm-1 can be attributed to O–H bending of absorbed water and C–O–C stretching of pyranose and glucose ring skeletal vibration, respectively.Figure 3
FT-IR spectra of CNC and ZnO/CNC nanohybrids.Compared to CNC, the nanocrystalline character of the obtained ZnO nanoparticles in the nanohybrids was confirmed by a new absorption band at 440 cm-1, assigned to Zn–O stretching vibration. With increasing concentration of Zn2+ ions, a slight shift of the Zn–O stretching bands to higher wavenumber was observed due to the lattice parameter of the ZnO nanoparticles. Besides, in comparison to CNC, the band intensity of C=O stretching vibration (1,721 cm-1) decreased, which was ascribed to strong interactions between oxygen atoms of carboxyl groups on the surface of the CNC and ZnO nanoparticles.The DRS curves of the ZnO and ZnO/CNC nanohybrids are presented in Figure4(a). The figure shows the strong absorption edge around 400 nm for all the ZnO/CNC nanohybrids. Based on the Tauc plot in Figure 4(b), the band gap values of them range about 3.1-3.2 eV. This result implies that these materials could absorb the irradiation light at wavelength of 254 nm in photocatalysis process.Figure 4
UV-vis diffuse reflectance spectra (a) and band gap determination from Tauc plot (b) of ZnO and nanohybrids.
(a)
(b)


#### 3.1.2. Morphology and Surface Area

FE-SEM images of the CNC and ZnO/CNC nanohybrids are shown in Figure5. It can be observed that the CNC appeared as rod-like monocrystals of 20 nm in diameter and 240–300 nm in length. After the precipitation process between CNC and Zn2+ ions, well-dispersed ZnO nanoparticles with average diameter of 50.2 nm and narrow size distribution (15–85 nm) were anchored on the surface of the CNC (ZnO/CNC-1.0). With increasing concentration of Zn2+ ions, the average diameter of the ZnO nanoparticles increased to 126.6 nm in ZnO/CNC-2.0.Figure 5
FE-SEM images of CNC and ZnO/CNC nanohybrids.The increase of Zn2+ ion concentration on the surface of the CNC enhances more Zn2+ ions anchored on CNC. This leads to an increase of ZnO particles not only in number but also in size on the surface of the CNC.The formation of ZnO on CNC can be explained as follows. During the CNC hydrolysis, the hydronium ions (H3O+) from HCl catalysts were used to catalyze the esterification of hydroxyl groups on the exposed cellulose chains with carboxyl groups of citric acid (C6H8O7). This reaction leads to the existence of carboxyl groups (−COOH) on the surface of citrate CNC. Then, Zn(NO3)2.6H2O solutions were added, and Zn2+ ions would be adsorbed on COO- negative site CNC surface due to the strong electrostatic interactions of these ions. When the Zn2+ concentration was low, the concentration of the carboxylate anion (COO-) from carboxyl groups on the CNC template was high. All Zn2+ ions were significantly located at these negative site, bonded to the anionic OH- to form the monomer zinc hydroxide Zn(OH)2 and two adjacent Zn(OH)2 dehydrated to form zinc oxide (ZnO) particles. As the result, the growth of ZnO crystals on CNC surface deduces an increase of the surface area. Figure 6 shows the nitrogen adsorption–desorption isotherms and the values of specific surface areas (SBET) of samples with different concentrations of ZnO.Figure 6
Nitrogen adsorption–desorption isotherm curves for CNC, ZnO/CNC-0.5, ZnO/CNC-1.0, and ZnO/CNC-2.0 nanohybrids.At light zinc-precursor Zn(NO3)2.6H2O, the synthesized nanohybrids showed an increased surface area compared with CNC. The SBET values of the CNC, ZnO/CNC-0.5, and ZnO/CNC-1.0 were 69.1, 73.1, and 74.8 m2·g-1, respectively.As the Zn2+ concentration increased, there are a large amount of Zn(OH)2 monomers formed in bulk solution, not anchored on the surface of the CNC. After that, the new bonds are produced after two Zn(OH)2 monomer dehydration to assemble along a certain orientation direction. The ZnO crystals grew continuously and eventually formed a sheet-like structure with the average diameter of the ZnO nanoparticles increased to 126.6 nm diameter, as show in FESEM image of ZnO/CNC-2.0 nanohybrid. The assembly of ZnO nanoparticles into two-dimensional sheet-like structure of CNC template led to a decrease of surface area as indicated in BET results of ZnO/CNC-2.0 nanohybrid (Figure 6).


### 3.2. Thermal Properties of CNC and ZnO/CNC Nanohybrids

Thermogravimetric analysis (TGA) has been used to characterize the thermal behavior of the CNC and ZnO/CNC nanohybrids. The thermal degradation onset temperature (To), maximum degradation temperature (Tmax), and apparent activation energy (Ea) are listed in Table 2.Table 2
To, Tmax, and Ea parameters for CNC and ZnO/CNC nanohybrids.
Sample
To (°C)a
Tmax (°C)a
Ea (kJ·Mol-1)b
CNC
293.3
376.7
128.7
ZnO/CNC-0.5
327.7
367.7
117.7
ZnO/CNC-1.0
329.5
373.6
137.7
ZnO/CNC-2.0
321.6
357.4
176.0
aTo and Tmax calculated from TGA curves. bEa calculated by the Horowitz and Metzger method.Figure7 shows the thermal degradation of CNC and ZnO/CNC nanohybrids elaborated by the one main step of maximum degradation temperature. It shows that the CNC has the lowest thermal stability (Figure 7(a)). The To and Tmax values of the CNC were about 293.3 and 376.7°C, respectively. Thermal degradation of cellulose involves depolymerization, dehydration, and decomposition of glycosyl units followed by formation of a charred residue. The result indicates that inorganic nanomaterials can significantly improve the thermal stability of polymer matrix. In comparison to CNC, the thermal degradation curves of the ZnO/CNC nanohybrids with a single degradation peak have shifted to higher temperature (Figure 7(b)). Furthermore, the To and Tmax values increased to 327.7 and 367.7°C for ZnO/CNC-0.5, 329.5 and 373.6°C for ZnO/CNC-1.0, and 321.6 and 357.4°C for ZnO/CNC-2.0. In particular, ZnO/CNC-1.0 showed the best thermal stability, with the increase of To (36.2°C) as compared to neat CNC. This was ascribed to the stronger interactions between oxygen atoms of the CNC and ZnO nanoparticles, thus providing a thermal barrier for the cellulose skeleton. Besides, there was no further decomposition of ZnO nanoparticles after cellulose degradation, whereas an increase in the char fraction was observed for the ZnO/CNC nanohybrids (Figure 7(a)). This indicates that thermally stable inorganic ZnO nanoparticles can act as a barrier flame retardant for the cellulose skeleton based on heat absorbance, resulting the higher degradation temperature and increased weight residue.Figure 7
TGA (a) and differential thermogravimetry (DTG) (b) curves for CNC and ZnO/CNC nanohybrids.
(a)
(b)The apparent activation energy (Ea), corresponding to interaction between CNC and ZnO nanoparticles, was calculated from the TGA data by using the Horowitz and Metzger method [27] as follows:
(6)lnlnWoWT=EaθRTS2,where Wo is the initial sample weight, WT is the residual sample weight at temperature T, TS is the temperature determined at 36.79% weight loss, θ is T−TS, and R is the gas constant.Figure8 shows the plots of lnlnWo/WT versus θ for the main stage of the thermal degradation of the CNC and ZnO/CNC, where Ea can be calculated from the slopes of the fit lines, and the results are listed in Table 2. In general, with the higher Ea value, the faster degradation rate occurs [27]. Table 2 illustrates that ZnO/CNC-2.0 showed the highest Ea value comparing to that of CNC and the other nanohybrids. Based on above results, this can be seen that the addition of ZnO nanoparticles to CNC matrix may lead to the degradation behavior of CNC in two opposite ways. On the one hand, during degradation of CNC, ZnO nanoparticles enhance the thermal stability of materials, so that the onset degradation temperature of the CNC gets higher, which is the factor resulting in increasing degradation rate of CNC. On the other hand, having strong interaction between ZnO nanoparticles with carboxyl groups of CNC, the thermally stable ZnO nanoparticles, as a flame retardant, will cover the surface of CNC and increase the CNC thermal degradation temperature.Figure 8
Plot oflnlnWo/WT versus θ for the main stage of thermal degradation of CNC and ZnO/CNC nanohybrids.


### 3.3. Adsorption and Photocatalytic Properties

#### 3.3.1. Adsorption

Because CNC surface has negative charges originating from its abundant carboxyl groups, the cationic MB molecules are adsorbed on the anionic CNC in the mixture through electrostatic interactions. This is the reason why the CNC has high adsorption ability in the dark. The MB adsorption results in Figure9 show a comparison of dye removal ability of CNC and ZnO/CNC nanohybrids. The dye removal ability of 40.22% and 28.15% for ZnO/CNC-1.0 and ZnO/CNC-2.0, respectively, was lower than that of CNC. Meanwhile, ZnO/CNC-0.5 gave the best adsorption of dye removal (73.62%), which is higher than that of CNC. At the same time, the dye removal ability of the nanohybrids decreased with the increase of ZnO, indicating the more ZnO contents on CNC and the weaker dye absorption ability. It can be explained that since the low content of ZnO in ZnO/CNC-0.5 will make the CNC surface more roughness, creating the high specific surface area of the material leads to the enhancement of MB adsorption ability (73.62%). With the light increase of ZnO content in ZnO/CNC-1.0 and ZnO/CNC-2.0 samples, the nanoparticles were found to be well dispersed and cover the CNC surface and effect negatively the adsorption ability (40.22% and 28.15%) by hindering the interaction between MB and CNC carboxyl groups.Figure 9
Dye removal of CNC and ZnO/CNC hybrids.


#### 3.3.2. Photocatalyst

The results for the photocatalytic activity are shown in Figure10. It can be seen that the ZnO/CNC nanohybrids possessed photocatalytic activity to decompose the dye under UV irradiation. If there was no presence of ZnO, no remarkable change in UV absorption band of MB during 1.0 h of UV irradiation was observed and then after 2.5 h UV exposure, only 2% degradation of MB has occurred. As shown in Figure 10, under UV irradiation, about 95% of the MB dye was decomposed fast after irradiation during 150 min in ZnO/CNC-1.0 nanohybrid. This observation indicates that the ZnO/CNC-1.0 nanohybrid, having well dispersion at small size of ZnO nanoparticles on the CNC surface, possessed high photocatalytic activity due to the strong electronic interaction between the CNC and ZnO nanoparticles. Photocatalysis occurs as ZnO photocatalyst is irradiated by light with energy larger than its band gap energy. After absorbing excited energy, photogenerated electron–hole pairs would be produced and migrated to the surface of ZnO nanoparticles (Equation (7)). Then, these e- and h+ can react with O2 and H2O absorbed on its surface to generate active radical OH⋅ (Equation (8)) and O2- (Equation (9)), which could participate in dye degradation by direct oxidation [11, 22, 28].
(7)ZnO+hυ⟶ZnOesur−+hsur+(8)h++H2OOH−⟶OH⋅(9)e−+O2⟶O2−Figure 10
Photocatalytic and adsorption activity of ZnO and ZnO/CNC nanohybrids.The other authors also achieved the similar results in the nanohybrids of ZnO/CNC and ZnO/reduced graphene oxide (RGO) [29] (Table 3). It is noted that the photocatalytic activity of the ZnO/CNC-1.0 nanohybrid was higher than that for ZnO nanoparticles coated on nanocellulose from oil palm empty fruit bunches [22].Table 3
The comparison of MB photocatalytic activity of the various ZnO/CNC nanohybrids.
Materials
The apparent first-order rate constant (min-1)
Ref.
ZnO/CNC-1.0
0.0157
This study
ZnO nanoparticles coated on nanocellulose from oil palm empty fruit bunches
0.0019
[22]
Sheet like(ZnO–CNC) nanohybrid synthesized by using hydrothermal method
0.016
[30]
Reduced graphene oxide–ZnO nanorod composites
0.039
[29]
ZnO/CNC synthesized by using hydrothermal method
0.028
[31]
ZnO–cellulose nanocomposite films from a cellulose–NaOH/urea/zincate solution
0.046
[32]In resuming results of both photocalytic and adsorption activities, the higher photocatalytic ability of ZnO/CNC nanohybrids was obtained, the lower adsorption activity was observed.


## 3.1. Structure, Morphology, and Surface Area of CNC and ZnO/CNC Nanohybrids

### 3.1.1. Structure

The XRD diffractograms of every NFT treatment stage and the CNC, ZnO, and ZnO/CNC-0.5 nanohybrids are shown in Figure1. The results present their CNC monoclinic structure. The (101), (002), and (040) lattice planes were observed at 2θ=16.15, 22.65, and 34.88°, respectively. These peaks correspond to polymorph of cellulose I (indexed to the database of ID number COD 4114994) [24]. XRD profiles of the cellulosic products obtained at each stage were analyzed to monitor the evolution of the percentage crystalline index (CrI) of nanocellulose. The physical and mechanical properties of cellulose are influenced by its crystalline structure. CrI is one important parameter of the crystal structure. The pretreatment of cellulose fibers increases their CrI meaning to the increasing of ratio of crystalline and amorphous region and surface roughness as well. This made it easy for the adhesion of ZnO onto the cellulose matrix. From XRD analysis, the CrI of cellulose and pretreated fibers was calculated in using the amorphous subtraction method described by Equation (3) [25]:
(3)CrI%=I002−IamI002×100,where I002 is the maximum diffraction intensity of the characteristic peak (002) at around 22–23° and Iam is the diffraction intensity attributed to amorphous cellulose, which is the valley between peaks (101) and (002). Treatment of the fibers increased their % CrI. The calculated % CrI were 57.1, 62.3, 78.9, and 84.5% for biomass obtained after acid, PFA, bleaching treatments, and the final acid hydrolysis, respectively. From these results, it can be deduced that each treatment step affected the crystallinity of the materials. The CrI was found to have increased by 26.9% after the removal of lignin and hemicellulose. The increasing % CrI indicates an improvement of the cellulose crystallinity.Figure 1
XRD patterns of different treatment stages by acid, PFA, bleaching, and the final acid hydrolysis for NFT (a) and CNC, ZnO, and ZnO/CNC-0.5 nanohybrids (b).
(a)
(b)The XRD patterns of ZnO with high intensity of the peaks revealed the single-phase crystalline nature of the synthesized ZnO nanostructures. The characteristic peaks correspond to the (100), (002), (101), (102), (110), (103), (200), (201), and (112) planes of ZnO were observed at2θ=31.89, 34.60, 36.39, 47.62, 56.67, 62.97, 66.47, 68.11, and 69.17°, respectively. This result is similar to those reported previously [16, 17] and are consistent with the database of ID number COD 1011258 with a wurtzite hexagonal crystallographic lattice of ZnO. The ZnO/CNC-0.5 XRD profile displays two-phase structures corresponding to cellulose (∗) and ZnO (#) as indicated in Figure 1(b). In addition, the XRD diagrams of ZnO/CNC-1.0 and ZnO/CNC-2.0 nanohybrids were similar to that of ZnO/CNC-0.5 in Figure 2, indicating the successful synthesis of this material by the facile one-step route. It can be deduced from these profiles that the presence of well crystalline ZnO has not altered the crystal structure of the cellulose matrix.Figure 2
XRD patterns of ZnO and ZnO/CNC nanohybrids.The average crystallite size (D) of cellulose, pure ZnO, and ZnO in the nanohybrids was calculated from XRD analysis using Equation (4): the Debye-Scherrer formula:
(4)D=0.9λβcosθ,where D is the crystallite size corresponding to the (hkl) plane, λ is the wavelength of copper Kα X-ray radiation (1.5406 Å), β is the observed angular width at half maximum intensity (FWHM), and θ is the Bragg diffraction angle measured in radians. In this work, the most intense diffraction lines ((100), (002), and (101)) were selected to calculate the average size of ZnO crystals. The lattice parameters a, b, and c, which are related to the interplanar distance (d) and Miller indices h, k, and l, were calculated for pure ZnO and ZnO in the nanohybrids using Equation (5) to determine the influence of cellulose nanocrystals
(5)1d2=43h2+hk+l2a2+l2c2.The calculated lattice parameters are reported in Table1. As observed in literature [22], compared to pure ZnO, the crystallite size of ZnO in the nanohybrids is much smaller, and there is a slight increase in the value of lattice parameters. According to Sharma et al. [26], the increase in the value of lattice parameters indicates the stretching of unit cells due to adsorption of polymer molecular chains on the surface of ZnO nanostructures.Table 1
Structural parameters of ZnO nanocrystals.
Sample
Crystallite size (nm)
Interplanar distance (d) (nm)
Lattice parameters
Lattice constantc/a
a=b (nm)
c (nm)
ZnO (pure)
32.8
0.2461
0.5323
0.2833
0.5323
ZnO/CNC-0.5
6.8
0.2489
0.5359
0.2872
0.5359
ZnO/CNC-1.0
9.1
0.2498
0.5369
0.2883
0.5369
ZnO/CNC-2.0
10.3
0.2489
0.5359
0.2872
0.5359The chemical structure of CNC and ZnO/CNC nanohybrids was analyzed using FTIR spectroscopy as shown in Figure3. Similar characteristic bands corresponding to CNC were observed for the ZnO/CNC nanohybrids at 3,375 and 1,380 cm-1, which can be related to stretching and bending vibrations of hydroxyl groups, respectively. The peaks at around 1,630 and 1,060 cm-1 can be attributed to O–H bending of absorbed water and C–O–C stretching of pyranose and glucose ring skeletal vibration, respectively.Figure 3
FT-IR spectra of CNC and ZnO/CNC nanohybrids.Compared to CNC, the nanocrystalline character of the obtained ZnO nanoparticles in the nanohybrids was confirmed by a new absorption band at 440 cm-1, assigned to Zn–O stretching vibration. With increasing concentration of Zn2+ ions, a slight shift of the Zn–O stretching bands to higher wavenumber was observed due to the lattice parameter of the ZnO nanoparticles. Besides, in comparison to CNC, the band intensity of C=O stretching vibration (1,721 cm-1) decreased, which was ascribed to strong interactions between oxygen atoms of carboxyl groups on the surface of the CNC and ZnO nanoparticles.The DRS curves of the ZnO and ZnO/CNC nanohybrids are presented in Figure4(a). The figure shows the strong absorption edge around 400 nm for all the ZnO/CNC nanohybrids. Based on the Tauc plot in Figure 4(b), the band gap values of them range about 3.1-3.2 eV. This result implies that these materials could absorb the irradiation light at wavelength of 254 nm in photocatalysis process.Figure 4
UV-vis diffuse reflectance spectra (a) and band gap determination from Tauc plot (b) of ZnO and nanohybrids.
(a)
(b)


### 3.1.2. Morphology and Surface Area

FE-SEM images of the CNC and ZnO/CNC nanohybrids are shown in Figure5. It can be observed that the CNC appeared as rod-like monocrystals of 20 nm in diameter and 240–300 nm in length. After the precipitation process between CNC and Zn2+ ions, well-dispersed ZnO nanoparticles with average diameter of 50.2 nm and narrow size distribution (15–85 nm) were anchored on the surface of the CNC (ZnO/CNC-1.0). With increasing concentration of Zn2+ ions, the average diameter of the ZnO nanoparticles increased to 126.6 nm in ZnO/CNC-2.0.Figure 5
FE-SEM images of CNC and ZnO/CNC nanohybrids.The increase of Zn2+ ion concentration on the surface of the CNC enhances more Zn2+ ions anchored on CNC. This leads to an increase of ZnO particles not only in number but also in size on the surface of the CNC.The formation of ZnO on CNC can be explained as follows. During the CNC hydrolysis, the hydronium ions (H3O+) from HCl catalysts were used to catalyze the esterification of hydroxyl groups on the exposed cellulose chains with carboxyl groups of citric acid (C6H8O7). This reaction leads to the existence of carboxyl groups (−COOH) on the surface of citrate CNC. Then, Zn(NO3)2.6H2O solutions were added, and Zn2+ ions would be adsorbed on COO- negative site CNC surface due to the strong electrostatic interactions of these ions. When the Zn2+ concentration was low, the concentration of the carboxylate anion (COO-) from carboxyl groups on the CNC template was high. All Zn2+ ions were significantly located at these negative site, bonded to the anionic OH- to form the monomer zinc hydroxide Zn(OH)2 and two adjacent Zn(OH)2 dehydrated to form zinc oxide (ZnO) particles. As the result, the growth of ZnO crystals on CNC surface deduces an increase of the surface area. Figure 6 shows the nitrogen adsorption–desorption isotherms and the values of specific surface areas (SBET) of samples with different concentrations of ZnO.Figure 6
Nitrogen adsorption–desorption isotherm curves for CNC, ZnO/CNC-0.5, ZnO/CNC-1.0, and ZnO/CNC-2.0 nanohybrids.At light zinc-precursor Zn(NO3)2.6H2O, the synthesized nanohybrids showed an increased surface area compared with CNC. The SBET values of the CNC, ZnO/CNC-0.5, and ZnO/CNC-1.0 were 69.1, 73.1, and 74.8 m2·g-1, respectively.As the Zn2+ concentration increased, there are a large amount of Zn(OH)2 monomers formed in bulk solution, not anchored on the surface of the CNC. After that, the new bonds are produced after two Zn(OH)2 monomer dehydration to assemble along a certain orientation direction. The ZnO crystals grew continuously and eventually formed a sheet-like structure with the average diameter of the ZnO nanoparticles increased to 126.6 nm diameter, as show in FESEM image of ZnO/CNC-2.0 nanohybrid. The assembly of ZnO nanoparticles into two-dimensional sheet-like structure of CNC template led to a decrease of surface area as indicated in BET results of ZnO/CNC-2.0 nanohybrid (Figure 6).


## 3.1.1. Structure

The XRD diffractograms of every NFT treatment stage and the CNC, ZnO, and ZnO/CNC-0.5 nanohybrids are shown in Figure1. The results present their CNC monoclinic structure. The (101), (002), and (040) lattice planes were observed at 2θ=16.15, 22.65, and 34.88°, respectively. These peaks correspond to polymorph of cellulose I (indexed to the database of ID number COD 4114994) [24]. XRD profiles of the cellulosic products obtained at each stage were analyzed to monitor the evolution of the percentage crystalline index (CrI) of nanocellulose. The physical and mechanical properties of cellulose are influenced by its crystalline structure. CrI is one important parameter of the crystal structure. The pretreatment of cellulose fibers increases their CrI meaning to the increasing of ratio of crystalline and amorphous region and surface roughness as well. This made it easy for the adhesion of ZnO onto the cellulose matrix. From XRD analysis, the CrI of cellulose and pretreated fibers was calculated in using the amorphous subtraction method described by Equation (3) [25]:
(3)CrI%=I002−IamI002×100,where I002 is the maximum diffraction intensity of the characteristic peak (002) at around 22–23° and Iam is the diffraction intensity attributed to amorphous cellulose, which is the valley between peaks (101) and (002). Treatment of the fibers increased their % CrI. The calculated % CrI were 57.1, 62.3, 78.9, and 84.5% for biomass obtained after acid, PFA, bleaching treatments, and the final acid hydrolysis, respectively. From these results, it can be deduced that each treatment step affected the crystallinity of the materials. The CrI was found to have increased by 26.9% after the removal of lignin and hemicellulose. The increasing % CrI indicates an improvement of the cellulose crystallinity.Figure 1
XRD patterns of different treatment stages by acid, PFA, bleaching, and the final acid hydrolysis for NFT (a) and CNC, ZnO, and ZnO/CNC-0.5 nanohybrids (b).
(a)
(b)The XRD patterns of ZnO with high intensity of the peaks revealed the single-phase crystalline nature of the synthesized ZnO nanostructures. The characteristic peaks correspond to the (100), (002), (101), (102), (110), (103), (200), (201), and (112) planes of ZnO were observed at2θ=31.89, 34.60, 36.39, 47.62, 56.67, 62.97, 66.47, 68.11, and 69.17°, respectively. This result is similar to those reported previously [16, 17] and are consistent with the database of ID number COD 1011258 with a wurtzite hexagonal crystallographic lattice of ZnO. The ZnO/CNC-0.5 XRD profile displays two-phase structures corresponding to cellulose (∗) and ZnO (#) as indicated in Figure 1(b). In addition, the XRD diagrams of ZnO/CNC-1.0 and ZnO/CNC-2.0 nanohybrids were similar to that of ZnO/CNC-0.5 in Figure 2, indicating the successful synthesis of this material by the facile one-step route. It can be deduced from these profiles that the presence of well crystalline ZnO has not altered the crystal structure of the cellulose matrix.Figure 2
XRD patterns of ZnO and ZnO/CNC nanohybrids.The average crystallite size (D) of cellulose, pure ZnO, and ZnO in the nanohybrids was calculated from XRD analysis using Equation (4): the Debye-Scherrer formula:
(4)D=0.9λβcosθ,where D is the crystallite size corresponding to the (hkl) plane, λ is the wavelength of copper Kα X-ray radiation (1.5406 Å), β is the observed angular width at half maximum intensity (FWHM), and θ is the Bragg diffraction angle measured in radians. In this work, the most intense diffraction lines ((100), (002), and (101)) were selected to calculate the average size of ZnO crystals. The lattice parameters a, b, and c, which are related to the interplanar distance (d) and Miller indices h, k, and l, were calculated for pure ZnO and ZnO in the nanohybrids using Equation (5) to determine the influence of cellulose nanocrystals
(5)1d2=43h2+hk+l2a2+l2c2.The calculated lattice parameters are reported in Table1. As observed in literature [22], compared to pure ZnO, the crystallite size of ZnO in the nanohybrids is much smaller, and there is a slight increase in the value of lattice parameters. According to Sharma et al. [26], the increase in the value of lattice parameters indicates the stretching of unit cells due to adsorption of polymer molecular chains on the surface of ZnO nanostructures.Table 1
Structural parameters of ZnO nanocrystals.
Sample
Crystallite size (nm)
Interplanar distance (d) (nm)
Lattice parameters
Lattice constantc/a
a=b (nm)
c (nm)
ZnO (pure)
32.8
0.2461
0.5323
0.2833
0.5323
ZnO/CNC-0.5
6.8
0.2489
0.5359
0.2872
0.5359
ZnO/CNC-1.0
9.1
0.2498
0.5369
0.2883
0.5369
ZnO/CNC-2.0
10.3
0.2489
0.5359
0.2872
0.5359The chemical structure of CNC and ZnO/CNC nanohybrids was analyzed using FTIR spectroscopy as shown in Figure3. Similar characteristic bands corresponding to CNC were observed for the ZnO/CNC nanohybrids at 3,375 and 1,380 cm-1, which can be related to stretching and bending vibrations of hydroxyl groups, respectively. The peaks at around 1,630 and 1,060 cm-1 can be attributed to O–H bending of absorbed water and C–O–C stretching of pyranose and glucose ring skeletal vibration, respectively.Figure 3
FT-IR spectra of CNC and ZnO/CNC nanohybrids.Compared to CNC, the nanocrystalline character of the obtained ZnO nanoparticles in the nanohybrids was confirmed by a new absorption band at 440 cm-1, assigned to Zn–O stretching vibration. With increasing concentration of Zn2+ ions, a slight shift of the Zn–O stretching bands to higher wavenumber was observed due to the lattice parameter of the ZnO nanoparticles. Besides, in comparison to CNC, the band intensity of C=O stretching vibration (1,721 cm-1) decreased, which was ascribed to strong interactions between oxygen atoms of carboxyl groups on the surface of the CNC and ZnO nanoparticles.The DRS curves of the ZnO and ZnO/CNC nanohybrids are presented in Figure4(a). The figure shows the strong absorption edge around 400 nm for all the ZnO/CNC nanohybrids. Based on the Tauc plot in Figure 4(b), the band gap values of them range about 3.1-3.2 eV. This result implies that these materials could absorb the irradiation light at wavelength of 254 nm in photocatalysis process.Figure 4
UV-vis diffuse reflectance spectra (a) and band gap determination from Tauc plot (b) of ZnO and nanohybrids.
(a)
(b)


## 3.1.2. Morphology and Surface Area

FE-SEM images of the CNC and ZnO/CNC nanohybrids are shown in Figure5. It can be observed that the CNC appeared as rod-like monocrystals of 20 nm in diameter and 240–300 nm in length. After the precipitation process between CNC and Zn2+ ions, well-dispersed ZnO nanoparticles with average diameter of 50.2 nm and narrow size distribution (15–85 nm) were anchored on the surface of the CNC (ZnO/CNC-1.0). With increasing concentration of Zn2+ ions, the average diameter of the ZnO nanoparticles increased to 126.6 nm in ZnO/CNC-2.0.Figure 5
FE-SEM images of CNC and ZnO/CNC nanohybrids.The increase of Zn2+ ion concentration on the surface of the CNC enhances more Zn2+ ions anchored on CNC. This leads to an increase of ZnO particles not only in number but also in size on the surface of the CNC.The formation of ZnO on CNC can be explained as follows. During the CNC hydrolysis, the hydronium ions (H3O+) from HCl catalysts were used to catalyze the esterification of hydroxyl groups on the exposed cellulose chains with carboxyl groups of citric acid (C6H8O7). This reaction leads to the existence of carboxyl groups (−COOH) on the surface of citrate CNC. Then, Zn(NO3)2.6H2O solutions were added, and Zn2+ ions would be adsorbed on COO- negative site CNC surface due to the strong electrostatic interactions of these ions. When the Zn2+ concentration was low, the concentration of the carboxylate anion (COO-) from carboxyl groups on the CNC template was high. All Zn2+ ions were significantly located at these negative site, bonded to the anionic OH- to form the monomer zinc hydroxide Zn(OH)2 and two adjacent Zn(OH)2 dehydrated to form zinc oxide (ZnO) particles. As the result, the growth of ZnO crystals on CNC surface deduces an increase of the surface area. Figure 6 shows the nitrogen adsorption–desorption isotherms and the values of specific surface areas (SBET) of samples with different concentrations of ZnO.Figure 6
Nitrogen adsorption–desorption isotherm curves for CNC, ZnO/CNC-0.5, ZnO/CNC-1.0, and ZnO/CNC-2.0 nanohybrids.At light zinc-precursor Zn(NO3)2.6H2O, the synthesized nanohybrids showed an increased surface area compared with CNC. The SBET values of the CNC, ZnO/CNC-0.5, and ZnO/CNC-1.0 were 69.1, 73.1, and 74.8 m2·g-1, respectively.As the Zn2+ concentration increased, there are a large amount of Zn(OH)2 monomers formed in bulk solution, not anchored on the surface of the CNC. After that, the new bonds are produced after two Zn(OH)2 monomer dehydration to assemble along a certain orientation direction. The ZnO crystals grew continuously and eventually formed a sheet-like structure with the average diameter of the ZnO nanoparticles increased to 126.6 nm diameter, as show in FESEM image of ZnO/CNC-2.0 nanohybrid. The assembly of ZnO nanoparticles into two-dimensional sheet-like structure of CNC template led to a decrease of surface area as indicated in BET results of ZnO/CNC-2.0 nanohybrid (Figure 6).


## 3.2. Thermal Properties of CNC and ZnO/CNC Nanohybrids

Thermogravimetric analysis (TGA) has been used to characterize the thermal behavior of the CNC and ZnO/CNC nanohybrids. The thermal degradation onset temperature (To), maximum degradation temperature (Tmax), and apparent activation energy (Ea) are listed in Table 2.Table 2
To, Tmax, and Ea parameters for CNC and ZnO/CNC nanohybrids.
Sample
To (°C)a
Tmax (°C)a
Ea (kJ·Mol-1)b
CNC
293.3
376.7
128.7
ZnO/CNC-0.5
327.7
367.7
117.7
ZnO/CNC-1.0
329.5
373.6
137.7
ZnO/CNC-2.0
321.6
357.4
176.0
aTo and Tmax calculated from TGA curves. bEa calculated by the Horowitz and Metzger method.Figure7 shows the thermal degradation of CNC and ZnO/CNC nanohybrids elaborated by the one main step of maximum degradation temperature. It shows that the CNC has the lowest thermal stability (Figure 7(a)). The To and Tmax values of the CNC were about 293.3 and 376.7°C, respectively. Thermal degradation of cellulose involves depolymerization, dehydration, and decomposition of glycosyl units followed by formation of a charred residue. The result indicates that inorganic nanomaterials can significantly improve the thermal stability of polymer matrix. In comparison to CNC, the thermal degradation curves of the ZnO/CNC nanohybrids with a single degradation peak have shifted to higher temperature (Figure 7(b)). Furthermore, the To and Tmax values increased to 327.7 and 367.7°C for ZnO/CNC-0.5, 329.5 and 373.6°C for ZnO/CNC-1.0, and 321.6 and 357.4°C for ZnO/CNC-2.0. In particular, ZnO/CNC-1.0 showed the best thermal stability, with the increase of To (36.2°C) as compared to neat CNC. This was ascribed to the stronger interactions between oxygen atoms of the CNC and ZnO nanoparticles, thus providing a thermal barrier for the cellulose skeleton. Besides, there was no further decomposition of ZnO nanoparticles after cellulose degradation, whereas an increase in the char fraction was observed for the ZnO/CNC nanohybrids (Figure 7(a)). This indicates that thermally stable inorganic ZnO nanoparticles can act as a barrier flame retardant for the cellulose skeleton based on heat absorbance, resulting the higher degradation temperature and increased weight residue.Figure 7
TGA (a) and differential thermogravimetry (DTG) (b) curves for CNC and ZnO/CNC nanohybrids.
(a)
(b)The apparent activation energy (Ea), corresponding to interaction between CNC and ZnO nanoparticles, was calculated from the TGA data by using the Horowitz and Metzger method [27] as follows:
(6)lnlnWoWT=EaθRTS2,where Wo is the initial sample weight, WT is the residual sample weight at temperature T, TS is the temperature determined at 36.79% weight loss, θ is T−TS, and R is the gas constant.Figure8 shows the plots of lnlnWo/WT versus θ for the main stage of the thermal degradation of the CNC and ZnO/CNC, where Ea can be calculated from the slopes of the fit lines, and the results are listed in Table 2. In general, with the higher Ea value, the faster degradation rate occurs [27]. Table 2 illustrates that ZnO/CNC-2.0 showed the highest Ea value comparing to that of CNC and the other nanohybrids. Based on above results, this can be seen that the addition of ZnO nanoparticles to CNC matrix may lead to the degradation behavior of CNC in two opposite ways. On the one hand, during degradation of CNC, ZnO nanoparticles enhance the thermal stability of materials, so that the onset degradation temperature of the CNC gets higher, which is the factor resulting in increasing degradation rate of CNC. On the other hand, having strong interaction between ZnO nanoparticles with carboxyl groups of CNC, the thermally stable ZnO nanoparticles, as a flame retardant, will cover the surface of CNC and increase the CNC thermal degradation temperature.Figure 8
Plot oflnlnWo/WT versus θ for the main stage of thermal degradation of CNC and ZnO/CNC nanohybrids.


## 3.3. Adsorption and Photocatalytic Properties

### 3.3.1. Adsorption

Because CNC surface has negative charges originating from its abundant carboxyl groups, the cationic MB molecules are adsorbed on the anionic CNC in the mixture through electrostatic interactions. This is the reason why the CNC has high adsorption ability in the dark. The MB adsorption results in Figure9 show a comparison of dye removal ability of CNC and ZnO/CNC nanohybrids. The dye removal ability of 40.22% and 28.15% for ZnO/CNC-1.0 and ZnO/CNC-2.0, respectively, was lower than that of CNC. Meanwhile, ZnO/CNC-0.5 gave the best adsorption of dye removal (73.62%), which is higher than that of CNC. At the same time, the dye removal ability of the nanohybrids decreased with the increase of ZnO, indicating the more ZnO contents on CNC and the weaker dye absorption ability. It can be explained that since the low content of ZnO in ZnO/CNC-0.5 will make the CNC surface more roughness, creating the high specific surface area of the material leads to the enhancement of MB adsorption ability (73.62%). With the light increase of ZnO content in ZnO/CNC-1.0 and ZnO/CNC-2.0 samples, the nanoparticles were found to be well dispersed and cover the CNC surface and effect negatively the adsorption ability (40.22% and 28.15%) by hindering the interaction between MB and CNC carboxyl groups.Figure 9
Dye removal of CNC and ZnO/CNC hybrids.


### 3.3.2. Photocatalyst

The results for the photocatalytic activity are shown in Figure10. It can be seen that the ZnO/CNC nanohybrids possessed photocatalytic activity to decompose the dye under UV irradiation. If there was no presence of ZnO, no remarkable change in UV absorption band of MB during 1.0 h of UV irradiation was observed and then after 2.5 h UV exposure, only 2% degradation of MB has occurred. As shown in Figure 10, under UV irradiation, about 95% of the MB dye was decomposed fast after irradiation during 150 min in ZnO/CNC-1.0 nanohybrid. This observation indicates that the ZnO/CNC-1.0 nanohybrid, having well dispersion at small size of ZnO nanoparticles on the CNC surface, possessed high photocatalytic activity due to the strong electronic interaction between the CNC and ZnO nanoparticles. Photocatalysis occurs as ZnO photocatalyst is irradiated by light with energy larger than its band gap energy. After absorbing excited energy, photogenerated electron–hole pairs would be produced and migrated to the surface of ZnO nanoparticles (Equation (7)). Then, these e- and h+ can react with O2 and H2O absorbed on its surface to generate active radical OH⋅ (Equation (8)) and O2- (Equation (9)), which could participate in dye degradation by direct oxidation [11, 22, 28].
(7)ZnO+hυ⟶ZnOesur−+hsur+(8)h++H2OOH−⟶OH⋅(9)e−+O2⟶O2−Figure 10
Photocatalytic and adsorption activity of ZnO and ZnO/CNC nanohybrids.The other authors also achieved the similar results in the nanohybrids of ZnO/CNC and ZnO/reduced graphene oxide (RGO) [29] (Table 3). It is noted that the photocatalytic activity of the ZnO/CNC-1.0 nanohybrid was higher than that for ZnO nanoparticles coated on nanocellulose from oil palm empty fruit bunches [22].Table 3
The comparison of MB photocatalytic activity of the various ZnO/CNC nanohybrids.
Materials
The apparent first-order rate constant (min-1)
Ref.
ZnO/CNC-1.0
0.0157
This study
ZnO nanoparticles coated on nanocellulose from oil palm empty fruit bunches
0.0019
[22]
Sheet like(ZnO–CNC) nanohybrid synthesized by using hydrothermal method
0.016
[30]
Reduced graphene oxide–ZnO nanorod composites
0.039
[29]
ZnO/CNC synthesized by using hydrothermal method
0.028
[31]
ZnO–cellulose nanocomposite films from a cellulose–NaOH/urea/zincate solution
0.046
[32]In resuming results of both photocalytic and adsorption activities, the higher photocatalytic ability of ZnO/CNC nanohybrids was obtained, the lower adsorption activity was observed.


## 3.3.1. Adsorption

Because CNC surface has negative charges originating from its abundant carboxyl groups, the cationic MB molecules are adsorbed on the anionic CNC in the mixture through electrostatic interactions. This is the reason why the CNC has high adsorption ability in the dark. The MB adsorption results in Figure9 show a comparison of dye removal ability of CNC and ZnO/CNC nanohybrids. The dye removal ability of 40.22% and 28.15% for ZnO/CNC-1.0 and ZnO/CNC-2.0, respectively, was lower than that of CNC. Meanwhile, ZnO/CNC-0.5 gave the best adsorption of dye removal (73.62%), which is higher than that of CNC. At the same time, the dye removal ability of the nanohybrids decreased with the increase of ZnO, indicating the more ZnO contents on CNC and the weaker dye absorption ability. It can be explained that since the low content of ZnO in ZnO/CNC-0.5 will make the CNC surface more roughness, creating the high specific surface area of the material leads to the enhancement of MB adsorption ability (73.62%). With the light increase of ZnO content in ZnO/CNC-1.0 and ZnO/CNC-2.0 samples, the nanoparticles were found to be well dispersed and cover the CNC surface and effect negatively the adsorption ability (40.22% and 28.15%) by hindering the interaction between MB and CNC carboxyl groups.Figure 9
Dye removal of CNC and ZnO/CNC hybrids.


## 3.3.2. Photocatalyst

The results for the photocatalytic activity are shown in Figure10. It can be seen that the ZnO/CNC nanohybrids possessed photocatalytic activity to decompose the dye under UV irradiation. If there was no presence of ZnO, no remarkable change in UV absorption band of MB during 1.0 h of UV irradiation was observed and then after 2.5 h UV exposure, only 2% degradation of MB has occurred. As shown in Figure 10, under UV irradiation, about 95% of the MB dye was decomposed fast after irradiation during 150 min in ZnO/CNC-1.0 nanohybrid. This observation indicates that the ZnO/CNC-1.0 nanohybrid, having well dispersion at small size of ZnO nanoparticles on the CNC surface, possessed high photocatalytic activity due to the strong electronic interaction between the CNC and ZnO nanoparticles. Photocatalysis occurs as ZnO photocatalyst is irradiated by light with energy larger than its band gap energy. After absorbing excited energy, photogenerated electron–hole pairs would be produced and migrated to the surface of ZnO nanoparticles (Equation (7)). Then, these e- and h+ can react with O2 and H2O absorbed on its surface to generate active radical OH⋅ (Equation (8)) and O2- (Equation (9)), which could participate in dye degradation by direct oxidation [11, 22, 28].
(7)ZnO+hυ⟶ZnOesur−+hsur+(8)h++H2OOH−⟶OH⋅(9)e−+O2⟶O2−Figure 10
Photocatalytic and adsorption activity of ZnO and ZnO/CNC nanohybrids.The other authors also achieved the similar results in the nanohybrids of ZnO/CNC and ZnO/reduced graphene oxide (RGO) [29] (Table 3). It is noted that the photocatalytic activity of the ZnO/CNC-1.0 nanohybrid was higher than that for ZnO nanoparticles coated on nanocellulose from oil palm empty fruit bunches [22].Table 3
The comparison of MB photocatalytic activity of the various ZnO/CNC nanohybrids.
Materials
The apparent first-order rate constant (min-1)
Ref.
ZnO/CNC-1.0
0.0157
This study
ZnO nanoparticles coated on nanocellulose from oil palm empty fruit bunches
0.0019
[22]
Sheet like(ZnO–CNC) nanohybrid synthesized by using hydrothermal method
0.016
[30]
Reduced graphene oxide–ZnO nanorod composites
0.039
[29]
ZnO/CNC synthesized by using hydrothermal method
0.028
[31]
ZnO–cellulose nanocomposite films from a cellulose–NaOH/urea/zincate solution
0.046
[32]In resuming results of both photocalytic and adsorption activities, the higher photocatalytic ability of ZnO/CNC nanohybrids was obtained, the lower adsorption activity was observed.


## 4. Conclusions

Using a facile, one-step synthesis route, ZnO/CNC nanohybrids possessing high thermal stability were obtained. The ZnO nanocrystals of 50 nm dispersed quite homogeneously around the CNC due to the strong electrostatic interactions between Zn2+ ions and carboxyl groups of CNC. Moreover, the results also gave the evidence that the degradation of MB was contributed not only by the adsorption ability of CNC but also by the photocatalytic activity of ZnO. The competition between these two processes depends on the concentration ratio of zinc-precursor Zn(NO3)2.6H2O and CNC. The MB photodegradation of ZnO/CNC-1.0 was obtained as high as 95% during 150 min. Depending on the purpose of organic dye treatment, it was observed that the yield of adsorption and photocatalytic process could be controlled by changing the concentration ratio of zinc-precursor Zn(NO3)2.6H2O and CNC from 0.5 to 1.0. Such nanohybrids with improved properties show great potential for waste water treatment.



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*Source: 6185976-2020-03-17.xml*