# Roles of Titin in the Structure and Elasticity of the Sarcomere

Authors: Larissa Tskhovrebova; John Trinick
Journal: Journal of Biomedicine and Biotechnology
 (2010)

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## Abstract

The giant protein titin is thought to play major roles in the assembly and function of muscle sarcomeres. Structural details, such as widths of Z- and M-lines and periodicities in the thick filaments, correlate with the substructure in the respective regions of the titin molecule. Sarcomere rest length, its operating range of lengths, and passive elastic properties are also directly controlled by the properties of titin. Here we review some recent titin data and discuss its implications for sarcomere architecture and elasticity.


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## Body

## 1. Introduction

The complex but extremely ordered structure of the sarcomere is the elemental force-producing machinery of striated muscles. Recent studies of sarcomere assembly [1, 2], protein turnover [1, 3], and signalling cascades [4, 5] provide new insights into the spectrum of intermolecular interactions that support sarcomere structure and function. There is increasing evidence that many of sarcomere properties involve the giant protein titin [2, 6–10].The titin molecule is more than one micrometer long andin situ spans half the sarcomere, with the N-terminus in the Z-line and the C-terminus in the M-line (Figure 1(a)) [11–13]. Different isoforms (MW ~ 3.0–3.7 MDa [14]) vary in the size and structure of the elastic I-band part of the molecule, which connects the end of the thick filament to the Z-line, as well as in the Z- and M-line regions. The size and structure of the thick filament part of titin is conserved, which is consistent with the conserved structure of thick filaments in vertebrates. Sequence shows that titin consists mainly of about 300 domains similar to immunoglobulins (Ig, I-set) and fibronectins (Fn, type-3). The elastic I-band part consists mainly of Ig domains arranged in tandem. Near the N2-line in the I-band, this arrangement is interrupted by unique sequences that bridge the “proximal” and “distal” (to the Z-line) tandem-Ig segments. In contrast, the thick filament part of titin is formed by both Ig and Fn3 domains.Schematic representation of titin layout in the sarcomere (a) and of the domain periodicity in the thick filament bound part (b). Striated zones in the sarcomere and the titin molecule (a) show the location of the large superrepeat.
(a)(b)Purified titin molecules visualized by metal shadowing appear in electron micrographs as strings about one micrometer in length and four nanometers in diameter [15–18]. In negatively stained samples, a distinct “beads-on-the-string” appearance can be seen [15], showing the chain of Ig and Fn3-like domains [19, 20].


## 2. Titin Structure—Conformational Periodicity in the Thick Filament Region

Sequence shows that the titin Ig and Fn3 domains are arranged in long-range patterns or super-repeats. Two types of super-repeats are found in the constitutively expressed thick filament region [19–21]: seven consecutive copies of the seven-domain or small superrepeat occupy the N-terminal part of this region; these are followed by eleven copies of the eleven-domain large superrepeat. In the I-band region, only the differentially expressed Ig-segments have super-repeats: in human soleus isoform, the N-terminal three copies of a six-domain superrepeat are followed by three copies of a ten-domain superrepeat [22, 23].  In both A- and I-band regions, the super-repeats show increased sequence conservation between domains at comparable positions in the super-repeats.The two super-repeats of Ig and Fn3 domains in A-band titin (Figure1(b)) closely reflect the underlying periodic structure of the thick filament. The size of the large superrepeat (~45 nm) is same as the myosin and C-protein periodicities of the filament, while the number of the large super-repeats (eleven) is the same as the number of binding sites for C-protein and related molecules. A single Ig domain in each large superrepeat binds C-protein and this defines the ~43 nm interval of C-protein localization in the C-zone [24]. Interactions between titin and myosin are probably grouped in three clusters of Fn3 domains, defining a period of ~15 nm in titin-myosin interactions [19].Atomic structures of recombinant titin fragments, supported by homology modelling [25, 26], indicate that the Ig and Fn3 domains are unlikely to be similarly oriented along a titin molecule but bend and twist relative to each other. The likelihood of periodic interactions with other thick filament proteins requires titin domains spaced by 43 nm to be in the same orientation. This indicates that in the A-band the orientation of Ig and Fn3 domains in the super-repeats is periodically repeated.There are only two main possibilities for such periodic architecture: either planar and zigzag-like or three-dimensional and helical. Homology modelling [26, 27] and crystallography studies [28, 29] show that small, two- or three-domain recombinant fragments have rather flat conformations, in which the long axes of the molecules bend in a single plane. However, longer segments may well have a tendency to bend three-dimensionally, as suggested by modelling of the periodic differentially expressed I-band Ig-segment [30]. The latter shape is supported by electron microscopy of purified titin also suggesting helicity [31]. A tendency of the relaxed titin molecule to adopt a helical conformation is likely to be controlled by a preferred inter-domain orientation [25, 30, 32] and may reflect a long-distance directional regularity in the bending and twisting angles. The existing atomic structures of titin fragments appear to be consistent with this possibility [30], although the number of these is still too small to discern a long-distance pattern.Another important question concerning titin interactions with other thick filament proteins is the significance of the fact that super-repeats in the A-band titin, although very similar, are not identical. Average sequence identity, even for domains at comparable positions, is below 40% [27], and only about 60% of their surface is conserved [33]. It is unclear at present how these small differences affect periodic interactions of titin with C-protein and myosin, both of which provide identical sites for interaction with super-repeats. It may be noted, however, that if there are dissimilar titin-myosin and titin-C-protein interactions in different super-repeats, this would agree with predictions of nonequivalency of the ~43 nm repeats in thick filament structure suggested by X-ray diffraction studies of muscle [34].


## 3. Titin Flexibility—Persistence Length of  the Elastic I-Band Region

Providing elasticity to sarcomeres is one of the major functions of titin [35–39]. This role derives from the ability of titin to increase the length under applied force and then to shorten to the original length when the force is removed. The mechanism of extensibility is known to be multiphase: overall shape changes that occur under small applied forces are followed at higher forces by hierarchical unfolding of the polypeptide [40–43]. While the physiological relevance of relatively large sarcomere lengths that lead to titin unfolding can be disputed, there is no doubt that conformational changes must occur in the molecule during both passive extension and active contraction of muscle. However, the exact pattern of these changes remains unknown. It is generally thought that they are entropic in nature and occur according to rod-coil transitions seen in individual titin molecules in vitro (e.g., [31]). However, the likely bundled state of titin molecules in the sarcomere is not usually taken into account [44–48]. In the following, we present some estimates related to titin flexibility in vitro and in situ.Electron and atomic force microscopy (AFM) of purified titin molecules illustrate a tendency to coil up in the absence of applied extensional force (Figure2(a)) [15, 16, 31, 49] or to straighten when a small pulling force is applied [17, 18, 31, 38, 50]. The persistence length (Lp) of monomeric titin is estimated to be 9–19 nm [31, 49, 51, 52]. A somewhat smaller range of values, 2–10 nm, was suggested by AFM and optical tweezer mechanical experiments on single molecules [43, 53–55], and a tendency of the protein to unfolding was discussed [53, 55].Electron micrographs of negatively stained titin, illustrating domain substructure and flexibility (a), and the effect of bundling on the apparent stiffness of the molecule (b). (a) Note that each of the flexibility “waves” seen in the titin contour contains about 4 domains. This number is comparable with the number of domains (3-4) expected for segments of about the persistence length of the protein. (b) This micrograph shows partially dissolved titin “end-filament”. The “wavy” contours of the molecules in the unbundled region contrast with their relatively straight shapes in the bundled part. Magnification: bar (a) 20 nm; (b) 50 nm.
(a)(b)The estimates ofLp are likely to mainly reflect average flexibility of the Ig/Fn3 parts of the molecule, since the unique sequences and other structures are a small proportion of the molecule. In a multidomain protein, overall bending flexibility mainly derives from the mobility at the inter-domain interfaces. Taking average interdomain distance s to be about 4.0 nm [27] and the average persistence length Lp to be about 13.5 nm, the average inter-domain angle can be approximately estimated from the relationship 〈θ2(s)〉=2s/Lp [56], from which a 〈θ〉 value of ~44° can be obtained. This value (i.e., 180° − 44° = 136°) is close to the average inter-domain angle in the NMR and crystal structures of titin fragments, ~140° [25, 26, 28–30, 32, 57, 58]. This suggests that the inter-domain angles and shapes of titin constructs reflect the equilibrium-relaxed conformation of the native titin molecule. In this conformation, the inter-domain angles and twists are likely to be different from those in situ, at least in the case of A-band titin, which is extended and stressed by the interactions with myosin and C-protein in the thick filament backbone.Only slightly higherLp values (15–40 nm) were estimated for individual titin molecules from mechanical experiments on muscle fibres [41] and myofibrils [59]. The difference between the in vitro and in situ results is smaller than might be expected since, in the sarcomere, the extensible I-band parts of titin are known to be bundled [44–48]. Inspection of the bundled and unbundled parts of I-band titin seen in electron microscope images (Figure 2(b)) clearly illustrates different bending properties. As was estimated earlier for an analogous case [60], the persistence length of a bundle scales with the square of the number of subfilaments in the bundle, that is, Lp ~ N2. In the case of titin, this means that a bundle of six molecules will make Lp at least 500 nm, that is, 36 times larger than Lp for a single molecule, taken as 13.5 nm. Even for smaller bundles, composed of only two-three molecules, the expected Lp value is in the region of ~55–120 nm.The twoLp values, 55–120 nm and 500 nm, reflect the expected stiffness of the two Ig-tandem segments of I-band titin that are separated by the unique N2-PEVK region: the proximal N-terminal segment, attached to the Z-line, and the bundled distal C-terminal segment, attached to the tip of the thick filament, known as the end-filament [44–48]. The fact that the Lp values from mechanical experiments on muscle fibres and myofibrils closely correspond to the in vitroLp value of the monomeric molecule is unlikely to correctly reflect titin’s state and flexibilityin situ. Alternatively,this may indicate dissociation of end-filaments during experiments, possibly due to their fragility, or may reflect the specificity of conformational changes in situ, related, for instance, to helicity (see above) and/or the confined environment in the sarcomere.


## 4. Adaptation of Titin Organization in  the Sarcomere to the Symmetry Mismatch between Z- and M-Lines

How the arrangement of titin in the sarcomere accommodates the different symmetries of thick and thin filament lattices, and of the M- and Z-line regions, is a major unresolved problem in sarcomere structure. The number of six molecules bound to each half of thick filament [39, 47] correlates well with the threefold rotational symmetry of the filament [61], its three-stranded substructure [44, 62, 63], and with the hexagonal lattice of the M-line region [61, 64]. However, titin extension through the I-band, and especially its interaction with thin filaments near and within Z-line region, suggests a rearrangement to fit to the twofold rotational symmetry of thin filaments and the tetragonal lattice of the Z-line. The question is thus how titin molecules rearrange in the I-band.One of the factors that may affect this rearrangement is self-association (see also above). Titin self-association in the I-band is suggested by electron microscope studies of muscle and separate thick filaments, which show the ~100 nm stalk-like structures called end-filaments, referred to above, projecting from the ends of thick filaments [44–46]. It is also supported by in vitro observations of self-association of the titin segment from this region [48]. This includes the entire distal (with respect to the Z-line) tandem-Ig segment of I-band titin, from the thick filament tip up to the PEVK-N2 region. At this point the bundle is likely to branch, although not necessarily into single molecules.The relatively high negative charge on the PEVK (N2B) region(s) of titin would favour branching of the end-filaments, unless the charge is neutralised by interactions either with cations (e.g., Ca++ ions [65, 66]) or  with other cytoplasmic components. It should also be noted that the N2-PEVK-region is the site where thin filaments appear to rearrange from hexagonal to tetragonal packing, which then becomes especially ordered near and within the Z-line [67]. However, structural studies have so far failed to provide an unambiguous answer for the number of the branched subfilaments. Three alternatives end-filament branching schemes are possible: (1) into two subfilaments, each containing three titin molecules; (2) into three subfilaments with two titin molecules in each; and (3) into six subfilaments, each a single titin molecule. In the first case, such a division would give 1 : 1 ratio of titin to thin filaments, which would imply interaction of each thin filament with a single bundle of three titin molecules near and within the Z-line region. This would satisfy tetragonal Z-line symmetry; however, there would be no agreement with the twofold symmetry of thin filament. Splitting the end-filament into a larger number of subfilaments would not satisfy either the Z-line or thin filament symmetries.Thus, some asymmetry has to be assumed to exist in the titin arrangement and interactions in either the I-band or within the Z-line. A possible arrangement of titin in the Z-line has been discussed [47]. This scheme suggests splitting of end-filament into six individual titin molecules with only four of these molecules interacting side-by-side with two thin filaments of the same sarcomere and extending throughout the Z-line. The remaining two titins would attach to the tips of two incoming thin filaments of the adjacent sarcomere. This arrangement gives a 2 : 1 ratio of side-by-side interactions between titin molecules and thin filaments and is in agreement with both thin filament and Z-line symmetries. It would also provide the required mechanical balance in Z-line region and appears to be in a good agreement with structural data [68].Another possibility relates to involvement of small titin isoforms, for example, Novex-3, which are present in skeletal and cardiac muscles in varying amounts in parallel with the main full-length isoforms, but which span only half the I-band, between the Z-line and N2-line [14, 69]. This location potentially helps to resolve the problem of correlation of the number of titin molecules with the thin filaments and Z-line symmetries. However, the low amount of Novex-3 expressed by muscles [14] is apparently not compatible with this role of the protein. Also, involvement of additional isoforms would not eliminate the asymmetry in titin interactions in the I-band but will only shift the site of asymmetry from the Z-line to the N2-line region of sarcomere where the different titin isoforms meet.


## 5. Concluding Remarks

Although an enormous amount has been learnt about the properties of the titin molecule, bothin vitro and in situ, integrating this information to give a comprehensive picture is not straightforward. This is mainly because in no part of the sarcomere—A- or I-band, Z- or M-line—is the disposition of the components known in molecular detail. Much therefore remains to be learnt about sarcomere structure and titin layout and function, and how these are compromised in disease.



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*Source: 612482-2010-06-21.xml*