# Value of Time to Positivity of Blood Culture in Children with Bloodstream Infections

Authors: Fen Pan; Wantong Zhao; Hong Zhang
Journal: Canadian Journal of Infectious Diseases and Medical Microbiology
 (2019)

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## Abstract

Objective. This study was to investigate the microbiological characteristics and the relationship between the time to positivity (TTP) of blood cultures and different bacterial species and to assess the clinical value of TTP in children with bloodstream infections (BSIs). Methods. The TTP of all the blood cultures from children with suspected BSIs was retrospectively collected in 2016. The microbiological characteristics and the relationship between the TTP of blood cultures and different bacterial species were also analyzed. Results. A total of 808 strains were isolated from 15835 blood cultures collected, and 145 (17.9%) were Gram-negative, 636 (78.7%) were Gram-positive, and 27 (3.3%) were fungi. The bacteria were divided into definite pathogens (174), possible pathogens (592), fungi (27), and contaminants (15). The average TTP of all positive blood cultures was 30.97 and ranged from 3.23 h to 92.73 h. The TTP of Gram-negative strains was significantly shorter than that of Gram-positive strains (P<0.001) and fungi (P = 0.032). The mean TTP for E. coli (15.60 h) was shortest within the group of Gram-negative isolates, and the mean TTP for Streptococcus (17.34 h) within the group of Gram-positive isolates. Significant difference of the TTP was detected in methicillin-resistant vs methicillin-susceptible S. aureus, extended-spectrum beta-lactamases (ESBLs) positive vs negative Enterobacteriaceae, and extensive drug-resistant and non-XDR A. baumannii. The median TTP in patients with BSI was significantly shorter than in those without it (P<0.001). ROC curve analysis indicated that the TTP cutoff value of CoNS, S. aureus, E. coli, and K. pneumoniae was 22.72 h, 19.6 h, 18.58 h, and 16.43 h, respectively, with most sensitive and specific predictor of BSIs. Conclusions. Our data acknowledged that TTP is a valuable index for the early prognosis of BSIs. TTP not only provides additional utility as a general predictor of bacteria with smear result but also provides the implication of drug-resistant organisms.


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## Body

## 1. Introduction

Bloodstream infections (BSIs) represent a major threat to the public health. On the basis of data reported, the annual incidence of BSIs was 189 per 100 000 person-years and the age-adjusted death rate has risen over the past decade [1, 2]. Blood culture, as the golden standard, is necessary for the diagnosis of BSIs, which can provide the information of pathogens including bacteria and fungi [3, 4]. As a parameter of blood culture, time to positivity (TTP), which is defined as the length of time from the beginning of culture incubation to the detection of bacterial growth by an automated system [5], has been proposed as a diagnostic and prognostic tool for BSIs.The TTP is influenced by several factors including different bacterial species and initial bacterial concentration, which depends on the processing parameter. Under the similar inoculum, it is also well known that the speed of growth in culture media differs by the bacterial species [6]. Previous studies characterized the clinical value of TTP in Enterobacteriaceae, Streptococcus pneumoniae, Staphylococcus spp., and Candida spp. [7–11] and provided the early primary positive result to the clinicians. In addition, the early detection of organisms contributes to optimizing antimicrobial therapy for children [12, 13].Therefore, the purpose of this study was to investigate the microbiological characteristics and the relationship between the TTP of blood cultures and different bacterial species, and to assess the clinical value of TTP in children with BSIs.


## 2. Materials and Methods

### 2.1. Study Design and Patients

A retrospective study was conducted at the clinical microbiology department in Shanghai Children’s Hospital, one of the largest pediatric hospitals in Shanghai, China. Patients aged 1 to 14 years with suspected BSIs were enrolled between January 1st and December 31st in 2016. The clinical data including age, gender, department, clinical indexes, the species, and TTP of positive blood cultures were also recorded.BSIs were defined according to the 2012 Surviving Sepsis Campaign guidelines [14, 15]. Patients were excluded for following reasons: incomplete information, patients less than 1 year old, samples less than 2 ml or more than 6 ml in volume, more than two species isolated from one-side bottle.


### 2.2. Culture Procedures

Blood collection was performed with two bottles from two sides, respectively, and conducted based on the Clinical and Laboratory Standard Institute (CLSI) H03-A6 document (presently replaced by the GP41-A6 document) [16]. A blood volume of 2–6 ml was collected by venepuncture, after the skin had been cleaned with 70% alcohol and inoculated into a BacT/Alert aerobic blood culture bottle. The bottle was then incubated immediately in a BACTEC FX200-automated blood culture system (BD Biosciences, America). The blood culture status either positive or negative on day 7 of incubation was considered as the final result.The species of positive blood cultures were identified using the VITEK 2 Compact system (BioMérieux Inc., France). Definite pathogens, possible pathogens, and contaminants were defined as described previously [17]. Briefly, definite pathogens are organisms known to cause disease, such as Streptococcus agalactiae, Staphylococcus aureus, and Gram-negative organisms. Possible pathogens are organisms that can cause disease under special circumstances, such as coagulase-negative Staphylococcus (CoNS), α-haemolytic Streptococcus, and γ-haemolytic Streptococcus. Contaminants are organisms that rarely cause disease, such as Corynebacterium species and Propionibacterium species. Coagulase-negative Staphylococcus (CoNS) was divided into groups: one-side positivity (CoNS++) and two-side positivity (CoNS+). Antimicrobial susceptibility testing was performed by Kirby–Bauer disc diffusion, and the breakpoints used for interpretation were recommended by the Clinical and Laboratory Standards Institute (CLSI) 2017 [18].


### 2.3. Statistical Analysis

Statistical analysis was performed by using SPSS 19.0 for Windows (version 19.0; SPSS Inc., Chicago, IL, USA). The data were expressed as means ± standard deviations (SD) and were analyzed with Student’st-test. Categorical variables, expressed as numbers and percentages, were compared by the Chi-squared or Fisher’s exact test. A value of P≤0.05 was considered statistically significant. To test the prognostic value of TTPs to predict BSIs, the receiver operating characteristics (ROCs) were plotted using MedCalc 12.4.0 software.


## 2.1. Study Design and Patients

A retrospective study was conducted at the clinical microbiology department in Shanghai Children’s Hospital, one of the largest pediatric hospitals in Shanghai, China. Patients aged 1 to 14 years with suspected BSIs were enrolled between January 1st and December 31st in 2016. The clinical data including age, gender, department, clinical indexes, the species, and TTP of positive blood cultures were also recorded.BSIs were defined according to the 2012 Surviving Sepsis Campaign guidelines [14, 15]. Patients were excluded for following reasons: incomplete information, patients less than 1 year old, samples less than 2 ml or more than 6 ml in volume, more than two species isolated from one-side bottle.


## 2.2. Culture Procedures

Blood collection was performed with two bottles from two sides, respectively, and conducted based on the Clinical and Laboratory Standard Institute (CLSI) H03-A6 document (presently replaced by the GP41-A6 document) [16]. A blood volume of 2–6 ml was collected by venepuncture, after the skin had been cleaned with 70% alcohol and inoculated into a BacT/Alert aerobic blood culture bottle. The bottle was then incubated immediately in a BACTEC FX200-automated blood culture system (BD Biosciences, America). The blood culture status either positive or negative on day 7 of incubation was considered as the final result.The species of positive blood cultures were identified using the VITEK 2 Compact system (BioMérieux Inc., France). Definite pathogens, possible pathogens, and contaminants were defined as described previously [17]. Briefly, definite pathogens are organisms known to cause disease, such as Streptococcus agalactiae, Staphylococcus aureus, and Gram-negative organisms. Possible pathogens are organisms that can cause disease under special circumstances, such as coagulase-negative Staphylococcus (CoNS), α-haemolytic Streptococcus, and γ-haemolytic Streptococcus. Contaminants are organisms that rarely cause disease, such as Corynebacterium species and Propionibacterium species. Coagulase-negative Staphylococcus (CoNS) was divided into groups: one-side positivity (CoNS++) and two-side positivity (CoNS+). Antimicrobial susceptibility testing was performed by Kirby–Bauer disc diffusion, and the breakpoints used for interpretation were recommended by the Clinical and Laboratory Standards Institute (CLSI) 2017 [18].


## 2.3. Statistical Analysis

Statistical analysis was performed by using SPSS 19.0 for Windows (version 19.0; SPSS Inc., Chicago, IL, USA). The data were expressed as means ± standard deviations (SD) and were analyzed with Student’st-test. Categorical variables, expressed as numbers and percentages, were compared by the Chi-squared or Fisher’s exact test. A value of P≤0.05 was considered statistically significant. To test the prognostic value of TTPs to predict BSIs, the receiver operating characteristics (ROCs) were plotted using MedCalc 12.4.0 software.


## 3. Results

### 3.1. Characteristics of Microbiology

A total of 808 strains were isolated from 15835 blood cultures collected throughout the entire study period (Table1). Of the 808 positive strains, 145 (17.9%) were Gram negative, 636 (78.7%) were Gram positive, and 27 (3.3%) were fungi. CoNS was mainly isolated, accounting for 61.9% (500/808). K. pneumoniae and E. coli were common in Gram-negative organisms. The mean age of these patients was 3.91 ± 3.40 years with most patients being male (474, 58.7%).Table 1
TTP of 808 blood culture positive strains.
Microorganisms
Number of strains
Proportion (%)
Average of TTP
Minimum TTP
Maximum TTP
Gram negative
145
17.9
17.61 ± 9.69
3.23
68.63
K. pneumoniae
68
16.29 ± 9.02
3.23
60.23
E. coli
26
15.60 ± 7.36
9.05
38.67
P. aeruginosa
12
20.40 ± 5.59
5.77
27.05
A. baumannii
11
19.45 ± 11.92
9.48
44.67
Other negative
28
20.93 ± 12.74
7.80
68.63
Gram positive
636
78.7
33.84 ± 14.19
4.28
92.73
CoNS++
87
33.91 ± 10.42
9.91
70.22
CoNS+
413
37.51 ± 14.04
13.68
92.73
S. aureus
29
23.44 ± 8.79
12.30
46.57
Enterococcus
53
23.54 ± 9.43
8.40
44.93
Streptococcus
39
17.34 ± 8.18
4.28
45.07
Other positive
15
30.63 ± 15.19
12.77
63.65
Fungi
Candida spp.
27
3.3
35.68 ± 14.93
14.10
82.25
Total
808
30.97 ± 14.89
3.23
92.73
TTP: time to positivity; CoNS: coagulase-negativeStaphylococcus.The bacteria were divided into definite pathogens (174), possible pathogens (592), fungi (27), and contaminants (15).K. pneumoniae (68), S. aureus (29), and E. coli (26) were predominant organisms among definite pathogens. The CoNS was the predominant isolate in the possible pathogen group. After clinical assessment by clinicians, a majority of CoNS were considered as contaminants.


### 3.2. Relationship between TTP and Strains

The average TTP of all isolates was 30.97 h, with a range from 3.23 h to 92.73 h. The TTP of Gram-negative strains was significantly shorter than the TTP of Gram-positive strains (P<0.001) and fungi (P=0.032). Among the group of Gram-negative isolates, the mean TTP for E. coli (15.60 h) was shortest, while the average TTP was observed in Streptococcus spp. (17.34 h) within the group of Gram-positive isolates. Meanwhile, significant difference for the mean TTP was detected between CoNS++ and CoNS+ groups (P=0.002).The rate and distribution of each species throughout the 1, 2, and 3 days of culture are illustrated in Table2. All microorganisms except for CoNS and fungi grew within the 2 days. The blood cultures with detected definite pathogens always became positive with the first 1 day, while the blood culture with detected possible pathogen was positive within 24 h–48 h.Table 2
The rate and distribution of 808 positive strains in four time periods.
Microorganisms
Total
0–24 h
24–48 h
48–72 h
>72 h
No.
Proportion (%)
No.
Proportion (%)
No.
Proportion (%)
No.
Proportion (%)
CoNS+
413
61
14.8
263
63.7
78
18.9
11
2.7
CoNS++
87
13
14.9
65
74.7
9
10.3
0
0
S. aureus
29
18
62.1
11
37.9
0
0
0
0
Enterococcus
53
29
54.7
24
45.3
0
0
0
0
Streptococcus
39
33
84.6
6
15.4
0
0
0
0
Other positive
15
6
40
7
46.7
2
13.3
0
0
K. pneumoniae
68
61
89.7
6
8.8
1
1.5
0
0
E. coli
26
24
92.3
2
7.7
0
0
0
0
P. aeruginosa
12
11
91.7
1
8.3
0
0
0
0
A. baumannii
11
9
81.8
2
18.2
0
0
0
0
Other negative
28
20
71.4
7
25.0
1
3.6
0
0
Fungi
27
2
7.4
21
77.8
3
11.1
1
3.7
Total
808
287
35.5
415
51.4
94
11.6
12
1.5
CoNS: coagulase-negativeStaphylococcus.


### 3.3. Relationship between TTP and Common Resistant Phenotype

As shown in Table3, the mean TTP for methicillin-resistant Staphylococcus aureus (MRSA) was 17.37 h versus 26.63 h for methicillin-susceptible Staphylococcus aureus (MSSA) (P=0.010). The average TTP of ESBL-positive Enterobacteriaceae was shorter than that of negative isolates (13.77 h vs. 19.98 h, P=0.004), which was also found between extensive drug-resistant (XDR) and non-XDR A. baumanniii. No significant difference was detected in MRCNS vs. MSCNS and PNSP vs. PSSP.Table 3
Relationship between TTP and resistant phenotype.
Microorganisms
No.
Average of TTP
Minimum TTP
Maximum TTP
t
Value ofP
CoNS
MRCNS∗
402
36.55 ± 13.58
9.17
92.73
0.077
0.782
MSCNS∗
98
38.23 ± 13.31
14.07
86.35
S. aureus
MRSA∗
10
17.37 ± 4.25
12.3
35.1
7.671
0.010
MSSA∗
19
26.63 ± 8.94
14.1
46.57
S. pneumoniae
PNSP∗
3
17.91 ± 3.22
14.38
20.7
2.174
0.166
PSSP∗
11
19.35 ± 8.03
10.43
36.85
Enterobacteriaceae
ESBLs∗
59
13.77 ± 5.65
3.23
33.85
8.904
0.004
Non-ESBLs
36
19.98 ± 10.82
9.05
60.23
A. baumannii
XDR∗
5
13.09 ± 4.23
9.48
19.58
11.113
0.009
Non-XDR
6
24.74 ± 13.99
11.42
44.67
TTP: time to positivity; CoNS: coagulase-negativeStaphylococcus; MRSA: methicillin-resistant Staphylococcus aureus; MSSA: methicillin-susceptible Staphylococcus aureus; PNSP: penicillin-nonsusceptible S. pneumoniae; PSSP: penicillin-susceptible S. pneumoniae; ESBLs: extended spectrum beta-lactamases; XDR: extensive drug resistance; MRCNS: methicillin-resistant coagulase-negative Staphylococcus; MSCNS: methicillin-susceptible coagulase-negative Staphylococcus.∗Common resistant phenotypes in some bacteria in clinical process; the TTP of these bacteria could provide earlier detection of drug-resistant isolates, and it would also help clinicians to adjust the antibiotics based on characteristics of smear and TTP after the positivity alarm.


### 3.4. Clinical Value of TTP

The median TTP in patients with BSI was significantly shorter than the median in those without it (P<0.001). ROC curve analysis indicated that the TTP cutoff value of all CoNS+, S. aureus, K. pneumoniae, and E. coli was 22.72 h, 19.6 h, 18.58 h, and 16.43 h, respectively, with most sensitive and specific predictor of BSIs. The area under the ROC curve (AUC), sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of TTP to identify BSIs were also calculated (Table 4).Table 4
Predictive value of TTP of microorganisms from bloodstream infections.
Microorganisms
AUC
Cutoff (h)
Sensitivity (%)
Specificity (%)
PPV (%)
NPV (%)
CoNS+
0.928
22.72
88.57
93.33
93
89.09
S. aureus
0.874
19.6
66.67
100
100
75
K. pneumoniae
0.818
18.58
76.67
75
75.41
71.19
E. coli
0.792
16.43
80
83.33
92.30
80.64
TTP: time to positivity; CoNS: coagulase-negativeStaphylococcus; AUC: area under the ROC curve; PPV: positive predictive value; NPV: negative predictive value.


## 3.1. Characteristics of Microbiology

A total of 808 strains were isolated from 15835 blood cultures collected throughout the entire study period (Table1). Of the 808 positive strains, 145 (17.9%) were Gram negative, 636 (78.7%) were Gram positive, and 27 (3.3%) were fungi. CoNS was mainly isolated, accounting for 61.9% (500/808). K. pneumoniae and E. coli were common in Gram-negative organisms. The mean age of these patients was 3.91 ± 3.40 years with most patients being male (474, 58.7%).Table 1
TTP of 808 blood culture positive strains.
Microorganisms
Number of strains
Proportion (%)
Average of TTP
Minimum TTP
Maximum TTP
Gram negative
145
17.9
17.61 ± 9.69
3.23
68.63
K. pneumoniae
68
16.29 ± 9.02
3.23
60.23
E. coli
26
15.60 ± 7.36
9.05
38.67
P. aeruginosa
12
20.40 ± 5.59
5.77
27.05
A. baumannii
11
19.45 ± 11.92
9.48
44.67
Other negative
28
20.93 ± 12.74
7.80
68.63
Gram positive
636
78.7
33.84 ± 14.19
4.28
92.73
CoNS++
87
33.91 ± 10.42
9.91
70.22
CoNS+
413
37.51 ± 14.04
13.68
92.73
S. aureus
29
23.44 ± 8.79
12.30
46.57
Enterococcus
53
23.54 ± 9.43
8.40
44.93
Streptococcus
39
17.34 ± 8.18
4.28
45.07
Other positive
15
30.63 ± 15.19
12.77
63.65
Fungi
Candida spp.
27
3.3
35.68 ± 14.93
14.10
82.25
Total
808
30.97 ± 14.89
3.23
92.73
TTP: time to positivity; CoNS: coagulase-negativeStaphylococcus.The bacteria were divided into definite pathogens (174), possible pathogens (592), fungi (27), and contaminants (15).K. pneumoniae (68), S. aureus (29), and E. coli (26) were predominant organisms among definite pathogens. The CoNS was the predominant isolate in the possible pathogen group. After clinical assessment by clinicians, a majority of CoNS were considered as contaminants.


## 3.2. Relationship between TTP and Strains

The average TTP of all isolates was 30.97 h, with a range from 3.23 h to 92.73 h. The TTP of Gram-negative strains was significantly shorter than the TTP of Gram-positive strains (P<0.001) and fungi (P=0.032). Among the group of Gram-negative isolates, the mean TTP for E. coli (15.60 h) was shortest, while the average TTP was observed in Streptococcus spp. (17.34 h) within the group of Gram-positive isolates. Meanwhile, significant difference for the mean TTP was detected between CoNS++ and CoNS+ groups (P=0.002).The rate and distribution of each species throughout the 1, 2, and 3 days of culture are illustrated in Table2. All microorganisms except for CoNS and fungi grew within the 2 days. The blood cultures with detected definite pathogens always became positive with the first 1 day, while the blood culture with detected possible pathogen was positive within 24 h–48 h.Table 2
The rate and distribution of 808 positive strains in four time periods.
Microorganisms
Total
0–24 h
24–48 h
48–72 h
>72 h
No.
Proportion (%)
No.
Proportion (%)
No.
Proportion (%)
No.
Proportion (%)
CoNS+
413
61
14.8
263
63.7
78
18.9
11
2.7
CoNS++
87
13
14.9
65
74.7
9
10.3
0
0
S. aureus
29
18
62.1
11
37.9
0
0
0
0
Enterococcus
53
29
54.7
24
45.3
0
0
0
0
Streptococcus
39
33
84.6
6
15.4
0
0
0
0
Other positive
15
6
40
7
46.7
2
13.3
0
0
K. pneumoniae
68
61
89.7
6
8.8
1
1.5
0
0
E. coli
26
24
92.3
2
7.7
0
0
0
0
P. aeruginosa
12
11
91.7
1
8.3
0
0
0
0
A. baumannii
11
9
81.8
2
18.2
0
0
0
0
Other negative
28
20
71.4
7
25.0
1
3.6
0
0
Fungi
27
2
7.4
21
77.8
3
11.1
1
3.7
Total
808
287
35.5
415
51.4
94
11.6
12
1.5
CoNS: coagulase-negativeStaphylococcus.


## 3.3. Relationship between TTP and Common Resistant Phenotype

As shown in Table3, the mean TTP for methicillin-resistant Staphylococcus aureus (MRSA) was 17.37 h versus 26.63 h for methicillin-susceptible Staphylococcus aureus (MSSA) (P=0.010). The average TTP of ESBL-positive Enterobacteriaceae was shorter than that of negative isolates (13.77 h vs. 19.98 h, P=0.004), which was also found between extensive drug-resistant (XDR) and non-XDR A. baumanniii. No significant difference was detected in MRCNS vs. MSCNS and PNSP vs. PSSP.Table 3
Relationship between TTP and resistant phenotype.
Microorganisms
No.
Average of TTP
Minimum TTP
Maximum TTP
t
Value ofP
CoNS
MRCNS∗
402
36.55 ± 13.58
9.17
92.73
0.077
0.782
MSCNS∗
98
38.23 ± 13.31
14.07
86.35
S. aureus
MRSA∗
10
17.37 ± 4.25
12.3
35.1
7.671
0.010
MSSA∗
19
26.63 ± 8.94
14.1
46.57
S. pneumoniae
PNSP∗
3
17.91 ± 3.22
14.38
20.7
2.174
0.166
PSSP∗
11
19.35 ± 8.03
10.43
36.85
Enterobacteriaceae
ESBLs∗
59
13.77 ± 5.65
3.23
33.85
8.904
0.004
Non-ESBLs
36
19.98 ± 10.82
9.05
60.23
A. baumannii
XDR∗
5
13.09 ± 4.23
9.48
19.58
11.113
0.009
Non-XDR
6
24.74 ± 13.99
11.42
44.67
TTP: time to positivity; CoNS: coagulase-negativeStaphylococcus; MRSA: methicillin-resistant Staphylococcus aureus; MSSA: methicillin-susceptible Staphylococcus aureus; PNSP: penicillin-nonsusceptible S. pneumoniae; PSSP: penicillin-susceptible S. pneumoniae; ESBLs: extended spectrum beta-lactamases; XDR: extensive drug resistance; MRCNS: methicillin-resistant coagulase-negative Staphylococcus; MSCNS: methicillin-susceptible coagulase-negative Staphylococcus.∗Common resistant phenotypes in some bacteria in clinical process; the TTP of these bacteria could provide earlier detection of drug-resistant isolates, and it would also help clinicians to adjust the antibiotics based on characteristics of smear and TTP after the positivity alarm.


## 3.4. Clinical Value of TTP

The median TTP in patients with BSI was significantly shorter than the median in those without it (P<0.001). ROC curve analysis indicated that the TTP cutoff value of all CoNS+, S. aureus, K. pneumoniae, and E. coli was 22.72 h, 19.6 h, 18.58 h, and 16.43 h, respectively, with most sensitive and specific predictor of BSIs. The area under the ROC curve (AUC), sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of TTP to identify BSIs were also calculated (Table 4).Table 4
Predictive value of TTP of microorganisms from bloodstream infections.
Microorganisms
AUC
Cutoff (h)
Sensitivity (%)
Specificity (%)
PPV (%)
NPV (%)
CoNS+
0.928
22.72
88.57
93.33
93
89.09
S. aureus
0.874
19.6
66.67
100
100
75
K. pneumoniae
0.818
18.58
76.67
75
75.41
71.19
E. coli
0.792
16.43
80
83.33
92.30
80.64
TTP: time to positivity; CoNS: coagulase-negativeStaphylococcus; AUC: area under the ROC curve; PPV: positive predictive value; NPV: negative predictive value.


## 4. Discussion

BSIs currently pose a significant cause of nosocomial infections and contribute substantially to morbidity and mortality worldwide [19, 20]. However, it is not easy to establish the diagnosis of BSIs since early clinical signs are mostly non-specific and inconclusive. Blood culture is considered as an important tool to the diagnosis and illness monitoring of BSIs, which can provide intuitionistic and accurate etiological index [21]. Although the identified pathogen is still the gold standard to diagnose BSIs, TTP may differentiate pathogens and contaminants in early.In this retrospective study, we determined the TTP of 808 positive blood cultures from children. Mostly CoNS pathogens were isolated within 48 h. The shortest TTP was detected inK. pneumoniae (3.23 h) and longest TTP was in CoNS (92.73 h). As a previous study reported that few true BSIs were tested after 48 h of culture [22], our data found that mostly definite pathogens including S. aureus, S. pneumoniae, K. pneumoniae, and E. coli were detected within 48 h and thus recommended as definite pathogen causing severe sepsis. However, about 60% of positive blood cultures yielded CNS, which is comparable with most other recent studies [23, 24]. The TTP of single and double positive for CoNS differed significantly (P<0.05). Double positive for CoNS maybe the true pathogens that caused BSI, while mostly single CoNS was contaminant for indifference of blood culture by clinicians and inaccuracy blood sampling [25]. Despite other factors, the short TTP for blood culture can reminder us that it may be a true pathogen, and clinicians can make decision about the management of this patient.Furthermore, we found another phenomenon that the TTPs were significantly different between patients with BSIs caused by drug-resistance pathogens and patients caused by drug-susceptible pathogens, especially in MRSA, ESBLs-positiveEnterobacteriaceae, and XDR A. baumannii isolates. These results were similar to the findings of previous recent studies that used automated blood culture systems [7,26–28]. TTP could provide earlier detection of drug-resistant isolates, and it would also help clinicians to adjust the antibiotics based on characteristics of smear and TTP after the positivity alarm. Pardo et al. previously reported that few true BSIs were detected after 48 h of culture and thus recommended antibiotic de-escalation at 48 h [22].In addition, the clinical value of TTP varied in different species. The TTP concerning to definite pathogens (S. aureus, E. coli, and K. pneumoniae) were ≤19.6 h, ≤18.58 h, and ≤16.43 h, respectively, which were considered to be associated with best clinical prognosis. In general, one-side positive blood culture isolated with these strains is recommended as true pathogens that caused BSIs. However, on account of CoNS isolates, our study showed that the best cutoff of TTP was 22.72 h, which can distinguish the infections and containments. It was also similar to a report with TTP ≤23.6 h conducted by Lai et al. [29]. When isolated with CoNS with long TTPs, clinicians should combine other assistant examination including pro-calcitonin and clinical features.


## 5. Conclusions

Although the limitation of TTP always exists and the inoculum amount of blood from children can influence the TTP, our data acknowledged that TTP is a valuable index for early prognosis of BSIs. TTP not only provides additional utility as a general predictor of bacteria with smear result but also provides the implication of drug-resistant organisms, which is helpful to the adjustment of antibiotic therapy.



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*Source: 5975837-2019-01-10.xml*